LOCUS       DBMDSJ010000001        24664 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_868_length_24664_cov_2.349428, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000001 DBMDSJ010000000
VERSION     DBMDSJ010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24664)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_868_length_24664_cov_2.349428"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="ACZPAQ_00005"
     CDS             <1..444
                     /locus_tag="ACZPAQ_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR0902384.1"
                     /translation="PADGGSIPTGELAPVEGTPMDLRAGVRIGAWIDEDFPQLTMAGG
                     YDHNWVVDGPAGTLRPAARAWSEETGIVLAVSTTMPGVQFYTGNYLDGCPAGKGNAPY
                     GKRWGFCLETQFFPDAVHHPEFPSPVLRAGAEYRQETRFCFSVDK"
     gene            complement(499..933)
                     /locus_tag="ACZPAQ_00010"
     CDS             complement(499..933)
                     /locus_tag="ACZPAQ_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902385.1"
                     /translation="MDPSGALLSFIHKNAAMGVGAIPQAMSLPQSRAMVPALDRQLRE
                     YRAIAAQSQAYARAHGRRELRGPGTAALAMSAAMLRAQSMLDPSTSRLAERMIQGSTM
                     GAVQMTRRLHQLTGRADPALVELGQRLLRTEEQNIQEMKRFL"
     gene            complement(1227..3335)
                     /locus_tag="ACZPAQ_00015"
     CDS             complement(1227..3335)
                     /locus_tag="ACZPAQ_00015"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR0902386.1"
                     /translation="MSVNITEIFGSNVFSTDVMRERLPKGVFREVMDVMENGGNISIE
                     TADIVANAMKDWAVEKGATHYTHWFQPLTGITAEKHDSFLTRPENSRTLLQLTGKQLI
                     MGESDASSFPSGGLRSTHYARGYTAWDMTSPAFVKEMTAGTILCIPTIFVSYHGEALD
                     KKTPLLRSMQAISTQALRILRLFGNTAAKKVIPQVGSEQEYFLVDREKYLKRRDLIYT
                     GRTLFGAPSPKGQELEDQYFGVIRDRVGSFMADLNQELWKLGIPATTQHNEVAPAQHE
                     MAPIFTMCNLAVDQNQLTMETMKRVATRHGLVCLLHEKPYAGVNGSGKHNNWSIGTDT
                     GENLLDPGDTPNENLQFLLVLSCIMKAVDVHADLLRQSASCVGNEHRLGGQEAPPAII
                     SICLGDQLESVVDQIVSNADSIQLLASGKLDSGVSTVPVLSRDATDRNRTTPFAFTGN
                     KFEFRMVGSSDSIADANVTLNAIVAEALCDAADVLEQAPDFSSACSQLIHENMARHQR
                     IIFNGDGYSDAWVAEAARRGLPNLASLVDAVPALTTEKAVRLFTKFGIYTQSELEARA
                     EIMYETYIKVTKIEANTMIHMASKHYIPTVISYTTRLAQSITSVSAACPEADLSVQRK
                     LLTQISDYLSQASCALEQLKPLMERVDSIPGVKEMALAYRTAVVPAMDALRYPIDALE
                     LLVDKSAWPVPTYGDLMFEV"
     gene            3658..4449
                     /gene="dapF"
                     /locus_tag="ACZPAQ_00020"
     CDS             3658..4449
                     /gene="dapF"
                     /locus_tag="ACZPAQ_00020"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR0902387.1"
                     /translation="MHITKMHGLGNDYIYLNGLEGAPEDLPGLAVRLSDRHFGVGGDG
                     VICIRPGRGGDFTMEMYNADGSRGAMCGNGIRCVGKYVYDKGLTRKTCLTIDTDAGPR
                     VLELQVRGGRVEQVTVDMGIPALSPAVSVEAEGETYTVWPVDVGNPHGVVFCPAPEAV
                     PLERVGPILEGSGAFPQRINVEFAAVRTRERLEARVWERGSGITLACGTGACAVLAAA
                     VRTGRCGRRAAVRLPGGVLELDWDRDSGHIWMTGPAVTVFEGEVD"
     gene            4580..5893
                     /locus_tag="ACZPAQ_00025"
     CDS             4580..5893
                     /locus_tag="ACZPAQ_00025"
                     /inference="COORDINATES: protein motif:HMM:NF015515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR0902388.1"
                     /translation="MELLAAFAVFLVGTFACMALGWSLSWGLALGLVCFTLVGLRRGL
                     SLRALVRMAGEGARTSLVVLRILVLIGFLTALWRASGTIAFFVHTGLEWMHPSAFYLA
                     AFLLASALSMAFGSSFGVAGTAGVILAAIARSGGASLAVTGGAVLSGAYLGERLSPAS
                     SSAALTAALAGVEQHDFQLRMWRTTPLPLALSLVFYGGISLLHPIQQVDQTILRALEE
                     GFDLSWTVVLPAVILLVLPFFKVSAARSILVSCGAAAVLAVWEQGRPLAEVAWSCVAG
                     YVVEQPELTGILSGGGLLSMVNGMCIVLLSCASSGILNGAELLEPVKARLERMARRTG
                     LYVTTAAVSVASAALLCNQSIALVLTGQMMGESFRRSGKDGLLLSQALGNTAIVLAAV
                     VPWSIAGSVPLEAMGASALAFPFSFFLFAAPVCDWLCHRKQFQME"
     gene            6067..7212
                     /locus_tag="ACZPAQ_00030"
     CDS             6067..7212
                     /locus_tag="ACZPAQ_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR0902389.1"
                     /translation="MARKAVERNISYDDVRQLYYVSMELGRDENGKRIKQYRTFPTLT
                     AARNGLRDFHTQRERQAREPQDEVREEMDLAHWLEYWMESIVRPNRAETTVYAYQKII
                     DNHILPALGGIPMSRLSAKRIQRYYTDTQQAAGLSSNTMRRHHDLLSSALRSAVRQDM
                     LLASPMDRVEPPRSRVKEAFFYDNEELKQLYQLIEGHILELAVKMAGSLGMRREEICG
                     LKWENVDFRRRLVLVREARTAYGATIVQKETKNRASVRTLYLPEEVLLLLGREQERQE
                     EERARQGAVFNRTDHVILDHKGAPYSPNALSLAFTRFVRKNGLPRLTFHGLRHTFATI
                     ASCQGASLFDIGKALGHSTPATTGRIYTHLVDRTHEDLVLRVSDALK"
     gene            7300..7719
                     /gene="ruvX"
                     /locus_tag="ACZPAQ_00035"
     CDS             7300..7719
                     /gene="ruvX"
                     /locus_tag="ACZPAQ_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR0902390.1"
                     /translation="MRIMAIDYGDAHTGVAVSDPTGFLTGDVTTIHSRKAEVVLEELV
                     KLVQTYQVDELVMGFPRNMDGTEGPRAELYRTFAGRVEEATGLKPVLWDERRTTVDAH
                     RILFDAGKNGKKRKKTVDAVAASLILEGYLEFRRMRG"
     gene            7758..8045
                     /locus_tag="ACZPAQ_00040"
     CDS             7758..8045
                     /locus_tag="ACZPAQ_00040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR0902391.1"
                     /translation="MMLFQVTYTMLPGQRDVFLQMMRESGILDQIRREEGCLDYRYYL
                     PEEEDGTLLLVERWTGPEAQKAHMATSHMARLGQVKAQYVAETSVVTWALD"
     gene            8159..8992
                     /locus_tag="ACZPAQ_00045"
     CDS             8159..8992
                     /locus_tag="ACZPAQ_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR0902392.1"
                     /translation="MNIKTVHAVYFSGTGTTEKVVTTLAKDLAERLGAGYEACCFNLP
                     EARKQALSFGPEDLAVVGTPVYAGRVPNLLLPYIRDMIHGEGTLAVPVVLYGNRNYDD
                     GLMELRNVLRDNGFCPVSAGAFVGEHSFSRILGAGRPDGEDMALVGELGEKTADKVRG
                     LTELPGEAVHVNGCDPIRPYYTPRDRHGEPIKDFLKAKPVTDPDKCVKCGLCARLCPM
                     GSIDPADVSNVAGKCIKCCACVKKCPTGAKYFDHEGYLYHQHELEDQYAGRRAPSEIF
                     V"
     gene            8955..9674
                     /locus_tag="ACZPAQ_00050"
     CDS             8955..9674
                     /locus_tag="ACZPAQ_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR0902393.1"
                     /translation="MPDGGPPARSLSEAERAAEAAAREELCQVCRLLYQRGYTVGHDG
                     NVSLRLGEDQILVTPSGVGKGRLRPGMLVRCALSGEVLEGERHPSSEIPMHLLVYRER
                     PDVRAVVHAHPPMATAFAVCRRPLRERYLTETISGLGEVPVAPFALPSTQEVPDSVRP
                     FVRDHSAVLLANHGALTWGPDLCSAFDRMETVEQTARIYANVQLLGGGVELTEAQETA
                     LLGLAGRYAVLAGPRTEEESV"
     gene            9671..10747
                     /gene="mtnA"
                     /locus_tag="ACZPAQ_00055"
     CDS             9671..10747
                     /gene="mtnA"
                     /locus_tag="ACZPAQ_00055"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR0902394.1"
                     /translation="MTAQSQPENVRLGEDGESVVLLDQTRLPGRVEYRTVRDLKEMVE
                     AIRTLRVRGAPAIGVFAGFCLSALARQMERAGLDTPGLLRELDRKGKILVSSRPTAVN
                     LSWAVERMMDRASGLAGGAPEELAAALEAESRAIRREDEEMCRAIAEHGLSLLKAGDG
                     VLTHCNAGPLATSRYGTALGPILLAGERGIPLRVFADETRPLLQGARLTAWELHQAGV
                     DVTLICDNMASMVMKNGWVQACFVGCDRVAANGDAANKIGTSGVAILAKHYGIPFYVL
                     GPTSTIDLNCPDGDHIPIEQRDPEEIRSLWYREPMAPAGVKCFNPAFDVTHHSLISGI
                     ITEKGICRPPFPQSLAALFSNGAR"
     gene            complement(10814..12127)
                     /locus_tag="ACZPAQ_00060"
     CDS             complement(10814..12127)
                     /locus_tag="ACZPAQ_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR0902395.1"
                     /translation="MWFDQAILYQIYPLGLCGAPADNDGHTEHRILRLLDWVEHIKQL
                     GADTVLLNPVFDSDRHGYDTRDYNRLDPRLGTNEDLAQVCRAFHDAGLRVLLDGVFHH
                     VGRGFWAFRDVQEKKWNSPYRDWFYLNFDGDTDRGDGFWYENWEGHGELVKLNLHNPD
                     VVEYQFQAIRSWVEQFGIDGLRLDVAYCLPASYLKRLRAFTQTLKPGFVLVGEVLGGD
                     YNLWMGPELCHSVTNYECYKGLWSSLNDRNLFEIGHSLARQYGPEPWTLYKGAHLLSF
                     VDNHDVTRIASKLRDPAHLPLIYALLFGMPGTPALYYGSEWGISGEKAPCSDAGLRPA
                     LEKPEWNALTSWIARLAAARRGSPALREGSYRNVLLTNLQLIFERRTETERVLVAVNA
                     DSQPYTAHFDAGCGLAWDLISGQQHDFGGGSQLPPYSAFFWKMER"
     gene            complement(12336..12458)
                     /locus_tag="ACZPAQ_00065"
     CDS             complement(12336..12458)
                     /locus_tag="ACZPAQ_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902396.1"
                     /translation="MLSCIWIKVPERRPPMDRFNRILLLVIALAALVLPYLTRQ"
     gene            complement(12529..13122)
                     /locus_tag="ACZPAQ_00070"
     CDS             complement(12529..13122)
                     /locus_tag="ACZPAQ_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0902397.1"
                     /translation="MISVSHLTLSYGEKRVLEDFSLTLPERGVTVLSGPSGCGKTTLL
                     RCLAGLERPRSGRIQAPPPRETSLLFQEDRLFPWRTVEQHLMDVLPQSRWAEVPRWLA
                     LAELTGEEGSFPASLSGGMRRRLALVRALALGGQLYLLDEPFTGVDPARVRRLMDALR
                     ALDAPVLLSSHETLACSLADQVICLDGPPLRLAKGQV"
     gene            complement(13119..13901)
                     /locus_tag="ACZPAQ_00075"
     CDS             complement(13119..13901)
                     /locus_tag="ACZPAQ_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0902398.1"
                     /translation="MACPDRLDRALSLLLPPAFWLGVWQLGAWLVELHVEGRGNELLL
                     PYPATVLLALFRLARSPEFWWTALASLARIAAGMAGGAVLGGLLAALTCTSPWCRRLF
                     APVIRIVRATPVVSFILLVLLWTGRDQVPAVIAGLMVLPVVWENLTQGIRATDGQLLE
                     LARAYRFSRWKTVRLVYLPSLRPHLRSAAATAMGLAWKSGVAAEVLCLPRLGIGAQIS
                     RAKQTFETADLFAWTAVVILLSLLMERALTRLIRRPEGGKPL"
     gene            complement(13888..14967)
                     /locus_tag="ACZPAQ_00080"
     CDS             complement(13888..14967)
                     /locus_tag="ACZPAQ_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0902399.1"
                     /translation="MKLNKQLSLLLCGALMVSALAGCGGGKDSSSASGSASDSQSGSV
                     SSGASSSDSYVLPNVMVLSGPTGVGAAKLMADNEAVTPEAVANGEASPVINTATVEAD
                     NQAVSSALINGDVDIAAVATNVAASLSAKSEGAVQVLAVNTLGVLYILEKGNSVQSMA
                     DLEGRTVYATGQGANPEFVLNYLLTENGVDPANVDIQWMTAQEVTAKMASSEDGVCLL
                     PVPAATALMLQDAGVRQALSLTEEWSKVSSGQLAMGCIVARTDYIEENPQGVENFLNA
                     YQDSIAYMSDPANLEDAAQLVAQYGITPNAAIARSAIPQCNLVCLTGVEMKAVLEDYY
                     QVLYDADPASIGGVLPYDSFYYGVS"
     gene            complement(15013..15672)
                     /locus_tag="ACZPAQ_00085"
     CDS             complement(15013..15672)
                     /locus_tag="ACZPAQ_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR0902400.1"
                     /translation="MAPDLSDQERALLSRCPLFLGVEDALLRSLPALPGVTVEPFAAG
                     EVLYGPGQFRQSLGVILSGQVQVTNGSLSVSLLRAGELFGAATLYNDAPEFAAVLTAQ
                     SPCRVLFLPQETLDRLLGEEPLLRRNYLQYLTGRIRFLSGRLRSVAQTGAEGKLARYL
                     LTVPSGEPLRLSATDLAKRLGISRASLYRAFQALEDARLIRREGKSMYVLDPAGLEGV
                     L"
     gene            complement(15856..16512)
                     /locus_tag="ACZPAQ_00090"
     CDS             complement(15856..16512)
                     /locus_tag="ACZPAQ_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0902401.1"
                     /translation="MSRYLLFDLDGTLTESGEGIIRSVQYALEKMGKPETDVQKLRSF
                     IGPPLIPQFMSFSGMTREEAGQAVQFYRERYSTTGVFENRPYPGIPEMLQTLTDRGYV
                     LSVASSKPEHYVRQILEHFGLTPFFHEIVGATMDEKRTEKADVIEEALKRLGLSDQRD
                     RAIMVGDKEHDVLGAQKAGIPCVAVTYGYGSEKELGQVSPWRLVSSVAELGEYLLSLN
                     "
     gene            16658..16930
                     /locus_tag="ACZPAQ_00095"
     CDS             16658..16930
                     /locus_tag="ACZPAQ_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902402.1"
                     /translation="MTDHCGPGRRKTQAGRRSDPEEMLAKVQAAAEEMTMRRYVHMPP
                     EGRPCCPEGCLAALLGELNQIQDHQNQILAELLRALERMADALERR"
     gene            complement(17035..17667)
                     /locus_tag="ACZPAQ_00100"
     CDS             complement(17035..17667)
                     /locus_tag="ACZPAQ_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR0902403.1"
                     /translation="MFTCASCTIEACRSGQLDKMPKNCPMRDRAFFDSILEEYFKPEN
                     HDFFIASAATEAIGYCRWPRLREVVEFSKRMGYTRLGLAFCAGLHREAEVVDRVLRQN
                     GFQVASVICKTGSIPKDRAGVPPEYRVRPGTYEVMCNPIAQAELLNRVETQFNICLGL
                     CVGHDSLFYRYSQAPVTTLVAKDRATGNNPVAAIYCADSYFKTRLDADQP"
     gene            complement(17712..18266)
                     /locus_tag="ACZPAQ_00105"
     CDS             complement(17712..18266)
                     /locus_tag="ACZPAQ_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HIR0902404.1"
                     /translation="MQYKLTTPVAKEDLAPLRAGDTVLLSGTVYTARDAAHKRMMELL
                     DAGEALPFPVEGSAVYYVGPTPERPGQIIGSAGPTTSGRMDAYSPRLLDLGQAIMIGK
                     GARNQAVKEAVVRSGAVYLAALGGAGALMAASVEELEVICWEDLGCEAVRRLTVRDLP
                     LTVILDAHGGDLYESGPAAYLSSL"
     gene            complement(18305..19132)
                     /locus_tag="ACZPAQ_00110"
     CDS             complement(18305..19132)
                     /locus_tag="ACZPAQ_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0902405.1"
                     /translation="MEEKMIAVDGRKLHVSDTGSGPVTLLILSGSNILFPSLEYAPLA
                     DALSPWYSVLIPSKFGYGHSDLTEAPRDVDTVVEEYRRVLSACGIPVPVVLAAHSMGF
                     LEALHWAFKYPEEVSALVGLDPAVPEVYQHMDLEAILSRLEALNRSGWKRTLLFWLLA
                     PALLGHCPAAERRRLMVDARRNFASAVWVNEARDLPRSVRQVEQEGPPLSHPTLFLLS
                     NGKGTPLAGDSWRACGQHYLSALPDSSLRTFDLPHDLYRRRPKELADTIHRFLLEHL"
     gene            complement(19155..19994)
                     /locus_tag="ACZPAQ_00115"
     CDS             complement(19155..19994)
                     /locus_tag="ACZPAQ_00115"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR0902406.1"
                     /translation="MRTIQAAAITETVAKLCIQANTHLPGDIVAALDAARQSEPWPLA
                     RETLGLLWNNLELAEEKNLPVCQDTGMACVFVELGQEIHIEGEFEAAIHEGVRQGYGE
                     GYLRKSIVGDPLRRVNTGDNTPAAITLRLVPGDACKITVAPKGFGSENMSRLGMLKPA
                     DGVEGVKRFVVETVRLAGSNPCPPTVLGVGIGGSFDKAAYLAKHALLRPIDQRNPDPF
                     YAALETELLDEINALGVGPQGFGGKTTCLGLSIETAPTHVAGLPVAVNVSCHVTRRAA
                     AEL"
     gene            complement(20118..21689)
                     /locus_tag="ACZPAQ_00120"
     CDS             complement(20118..21689)
                     /locus_tag="ACZPAQ_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902407.1"
                     /translation="MKQDEFDLRLTQEMADLPPDPGEVQDYNPWQAAMSRILWGMALT
                     TFRLEFFYLQYLLPLLGAALMYLGYRSLRRENPWFRLCWGLSGLLLAIHIALDILAAT
                     PVMGWIAGNPAVNWGLTWPIRGMDLLLLFALWRGSRAAFRTVGEAQPPRDWLREGFLC
                     YLLALATALWSELVPATEPSLLGVTITNHWLYYLRPIAFLILEIRLLVCIAHQSDALA
                     GLGYDIAPAPVRVSARPFLLGVFALTLAAIPPALWLGGRIPTGPAEPAAQLTAEQEDI
                     RARLVSLGLPEDVAAALDGAELERCAQAEAVRAGRTYDTDHTDNETPRTDGPAVLHEL
                     GGGEAELSSWLLFLPEDQVRQIHFFRYRAMPDLHLQEQFSVDPSGNFHAEDYAGRLLW
                     EKNGQTLAVSPEIRLAGGQTAEELTEDQQLWYELELHRLGHLHYSPSFSFSIPRGAEG
                     MRGWIAYTTAAVPSPLVEDPSDWSYGDFWYVFLRHQERWLHYPFVSIRDLGGSRSAGA
                     YGPIRSVYAPFNFYP"
     gene            complement(21686..>22242)
                     /locus_tag="ACZPAQ_00125"
     CDS             complement(21686..>22242)
                     /locus_tag="ACZPAQ_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0902408.1"
                     /translation="QKEVTGMDPSAWMERLQQGDREALEPLLAHYEGMLRYIVSGVLS
                     DPHRQEDCLAEVRLKLWEKREQYDPERSSPATWLTVLCRNAAVDHLRSAQRHSAEALD
                     ETRPDPAPGPEEQLLRKERAERLERALASLSSRDRALFYRKYYYLQSTARIAAELGLT
                     PRAVEGRLYRIRKGLQNKLGGDLL"
     assembly_gap    22243..22252
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            22748..>24664
                     /locus_tag="ACZPAQ_00130"
     CDS             22748..>24664
                     /locus_tag="ACZPAQ_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR0902409.1"
                     /translation="MTDLFEKSIATLELPRVLEQLAACASTQEGKERCLALRPMTDPD
                     DVQRAQEETSAAVEMLIKRGSPGFSGVKPVGASLHRADMGGSLNTRELLEVAAVLRCA
                     RTVQDYGAGEEKTPISHLFRALTPNRFLEETITNSIIGEDELADSASSELASIRRHIR
                     ATEAKVRDILQRILASNQSKYLQESIITIRSDRYVVPVKSEFKNAIPGLVHDVSSSGS
                     TFFIEPMGVVKANNELRELMAQEKKEIERILAELSAQCAAHREDIAEDYDLLVWLDAI
                     FARGRLSLNMEAAQPRLSDRYLRLRKARHPLLDRKKAVANDLELGDRFDTLMITGPNT
                     GGKTVTLKTIGLLTLMAQCGLHIPTGSDSTVRIFDRVLADIGDEQSIAQSLSTFSSHM
                     TNIVGILQEADDRTLILFDELGAGTDPVEGAALAAAIIESARGIGALVAATTHYAELK
                     VYAMTTAGVENASCEFNVETLAPTYRLILGIPGKSNAFAISRRLGLPEYIIEKAAARL
                     DAENVRFEDVLTKLDQQRQEMEKERTEARRLKLEMEQSAAKAREYRERLEAERAKVVE
                     KAQAEARSIIQEARAASDLAISELKELKKRKDLDWQQVNDGRAEARRLLNEAERGIGG
                     PVQEPEAPPPTREAR"
BASE COUNT         4343 a         7565 c         8684 g         4062 t
ORIGIN      
        1 cccgcggacg ggggctccat ccccacgggg gagctggccc cggtggaggg cacccccatg
       61 gacctgcggg cgggggtgcg gatcggcgcg tggatcgacg aggacttccc ccagctgacc
      121 atggcggggg gctatgacca caactgggtg gtggacggcc cggcggggac gctgcgcccg
      181 gcggcccggg cctggtcgga ggagacgggg atcgttctgg cggtgagcac caccatgccc
      241 ggcgtacaat tctacacagg caactatctg gacggctgtc ccgcggggaa ggggaacgcc
      301 ccctacggca agcggtgggg cttctgcctg gagacccagt tcttcccgga cgccgtccac
      361 catccggagt tccccagccc ggtgctgcgg gccggggcgg agtaccggca ggagacccgg
      421 ttctgcttct ccgtggacaa atgagcggct gcgggccgga ggcgtattgc ctccggcccg
      481 ctttcctctt cgtacggttc agaggaagcg cttcatctcc tggatgttct gttcctcggt
      541 gcgcagcagc cgctgaccca gttccaccag ggccgggtcg gcccggccgg tgagctggtg
      601 aaggcggcgg gtcatctgga cggcgcccat ggtgctgccc tggatcatcc gctccgccag
      661 cctggatgtg gacgggtcca gcatggactg ggcccgcagc atggcggcgg acatggccag
      721 ggctgcggtg ccggggcccc gcagctcccg ccggccgtgg gcccgggcat aggcctggga
      781 ctgggcggcg atggcccggt actcccggag ctgccggtcc agggcgggga ccatggcccg
      841 gctctgaggc agcgacatgg cctgggggat ggcccccact cccatggcgg catttttgtg
      901 gatgaaggat aacagtgctc cgctgggatc caaacttctc acctccaggg cttagcctgc
      961 gccggaggcg gaaaaatatc cagaccagcc cgtcattttc tcactgatgg agccggacct
     1021 ggaggaggag gccataatcg agagaatgta tcctcccgaa tttgaggacg agatcgacgc
     1081 gctgcgggag gccaaggaga ggaaggccaa agacgggaag aagtaaattt acaacacagg
     1141 atagtttttg acaagcagag acgccccgga gcactgctcc ggggcgtctc tgcttgaaat
     1201 atcaacagat ggaaagggaa aacagcttac acctcgaaca tcaggtcgcc gtaggtgggc
     1261 actggccagg cggacttgtc caccagcagc tccagggcgt cgatgggata gcggagggcg
     1321 tccatggcag ggactacggc ggtacggtag gccagggcca tctccttcac gccggggatg
     1381 gagtccaccc gctccatcag gggcttgagc tgctctaggg cgcaggaggc ctgggagagg
     1441 tagtcgctga tctgggtcag cagcttgcgc tggacggaca ggtcggcctc aggacaggcg
     1501 gcggacaccg aggtgatgga ctgggccaga cgggtggtgt agctgatgac ggtggggatg
     1561 tagtgcttgg aggccatgtg gatcatggtg ttggcctcga tcttggtgac cttgatatag
     1621 gtctcataca tgatctccgc ccgggcctcc agctcagact gggtgtagat cccgaacttg
     1681 gtgaacagac ggaccgcctt ctccgtggtg agggcgggga ccgcatccac cagagaggcc
     1741 aggttgggca ggccgcggcg ggcggcctcg gccacccagg cgtcggagta gccgtcgccg
     1801 ttgaagatga tacgctgatg ccgggccatg ttttcgtgga tcagctggga gcaggcggag
     1861 gagaagtcgg gggcctgctc caggacgtcg gcggcgtcgc acagggcctc ggccacgatg
     1921 gcgttgaggg tgacgttggc gtcggcgatg gagtcggagg agcccaccat gcggaactcg
     1981 aacttgttgc cggtgaaggc gaagggggtg gtgcggttgc ggtcggtggc gtcccggctc
     2041 aggacgggga cggtggacac gccggagtcc agcttgccgg aggccagcag ctggatggag
     2101 tcggcgttgg agacgatctg gtccaccacg ctctccagct ggtcgcccag gcagatggag
     2161 atgatggcgg gaggggcctc ctggccgccc aggcggtgct cgttgcccac gcaggaggcg
     2221 gactgccgca gcaggtcggc gtggacgtcc actgccttca tgatgcagga gaggaccagc
     2281 aggaactgca ggttctcgtt gggggtgtcg ccggggtcca gcaggttctc gccggtgtcg
     2341 gtgccgatgg accagttgtt gtgcttgccg gagccgttga cgccggcata gggcttttcg
     2401 tggaggaggc agaccaggcc gtgacgggtg gccacccgct tcatggtctc catggtcagc
     2461 tggttctggt ccacggccag gttgcacatg gtgaagatgg gggccatctc atgctgggcg
     2521 ggggccacct cgttgtgctg ggtggtggcg gggatgccca gcttccacag ctcctgattc
     2581 aggtcggcca tgaaggagcc cacccggtcc cggatgacgc cgaagtactg gtcctccagc
     2641 tcctgcccct tgggggaggg tgcgccgaag agggtgcgcc cggtgtagat gaggtcccgg
     2701 cgcttcagat acttctcccg gtccaccagg aagtactcct gctcggaccc cacctggggg
     2761 atgacctttt tggccgcggt gttcccaaac agccggagga tgcgcagcgc ctgggtgctg
     2821 atggcctgca tggagcgcag caggggggtt ttcttgtcca gcgcctcgcc gtggtaggag
     2881 acgaagatgg tggggatgca caggatggtg ccggcggtca tctccttgac gaaggcgggg
     2941 gaggtcatgt cccaggcggt gtagccccgg gcgtagtggg tggagcgcag cccgccggag
     3001 gggaaggagg aggcgtcgga ctcccccatg atcagctgct tgccggtgag ctgaagcagg
     3061 gtgcggctgt tttccgggcg ggtgaggaag gagtcgtgct tctccgcggt gatgccggtg
     3121 agaggctgga accagtgggt atagtgggtg gcgccctttt ccaccgccca gtccttcatg
     3181 gcgttggcca cgatgtcggc ggtctcgatg ctgatgttgc cgccgttttc catcacgtcc
     3241 atgacctcgc ggaacacgcc cttggggagt cgctcccgca tcacatcggt gctgaataca
     3301 ttggaaccga agatctctgt gatattgacg ctcataggtt caatcctttc cattctgtat
     3361 ggtctgaccg cccggccaga ggggaagagg agcgggcggc ggctgccggg aggcgactgg
     3421 tccgccgccg cgctgcgggg gtgcatattg agatagatat cacatttaaa ttactccctg
     3481 ggcggggaaa atgcaagcct ttaatgtgac aaagttcggg ggcggcaggc ccaggggatg
     3541 tgggtgtttc cacaaaaagg gacggcggga aacagggcgg ggaaggctgc cgggccttct
     3601 gtcatacccg ggccgccggg cgcatagcct ttcccgtatg gaaaggagcg gtggacgatg
     3661 cacattacca aaatgcacgg gcttgggaac gactacatct atctgaacgg tctggagggc
     3721 gccccggagg atctgccggg gctggcggtg cgcctgtccg accggcattt cggggtgggg
     3781 ggcgacgggg tgatctgcat ccgtcccgga cggggcgggg acttcaccat ggagatgtac
     3841 aacgcggacg gctcccgggg ggccatgtgc ggcaacggga tccgctgtgt gggcaagtat
     3901 gtctatgaca aggggctgac ccgcaagacc tgcctgacca tcgacaccga cgccggcccc
     3961 cgcgttctgg agctgcaggt ccggggcgga cgggtggagc aggtcacggt ggatatgggg
     4021 atccccgcgc tgtcccccgc ggtgtccgtg gaggcggagg gggagaccta tacggtctgg
     4081 ccggtggatg tgggcaaccc acacggggtg gtgttctgcc ccgcgccgga ggcggtccct
     4141 ctggagcggg tggggcccat tctggagggg agcggggcgt tcccccagcg gatcaacgtg
     4201 gagttcgcgg cggtccggac ccgggagcgg ctggaggccc gggtgtggga gcgggggagc
     4261 gggatcaccc tggcctgcgg gacgggggcc tgcgcggtgc tggcggcggc ggtcaggacc
     4321 ggccggtgcg gacggcgggc cgcggtgcgg ctgccgggtg gcgtgctgga gctggactgg
     4381 gaccgggaca gcgggcacat ttggatgacc ggcccggcgg tgaccgtgtt cgagggggag
     4441 gtggactgaa ggctgcgctc aggctggaga agtacaaaaa gaatcattcg acaaacggga
     4501 tattttcctc tatactaaaa gacgacatca acaagcggcc ctctggggtg ccggagggcc
     4561 caaagaaggg gataggaccg tggagcttct ggccgcattt gctgtatttc tggttgggac
     4621 cttcgcctgt atggcgctgg ggtggtccct gtcctggggg ctggccctgg ggctggtgtg
     4681 cttcaccctg gtggggctgc gccgggggct gagcctccgg gcgctggtcc gtatggcggg
     4741 ggagggggcc cgcacctcgc tggtggtgct gcgcatcctg gtgctcatcg gctttctcac
     4801 cgccctgtgg cgggccagcg ggaccatcgc ctttttcgtc cacacgggcc tggagtggat
     4861 gcaccccagc gccttctatc tggcggcctt cctgctggcc tccgccctgt ccatggcctt
     4921 cggcagctcc ttcggcgtgg cggggacggc gggggtgatc ctggccgcca tcgcccgcag
     4981 cggcggggcc agcctggccg tcacaggggg cgcggtgctg tccggggcct acctggggga
     5041 gcggctgtcc ccggcctcct cctctgcggc cctgaccgcc gccctggccg gggtggagca
     5101 gcacgacttt cagctgcgga tgtggcggac cacgcctctg cccctggccc tctccctggt
     5161 attttacgga gggatctctc tgctgcaccc catccagcag gtggatcaga ccatcctgag
     5221 ggccctggag gagggctttg acctgtcctg gaccgtggtg ctgccggcgg tcatcctgct
     5281 ggtgctgccc tttttcaagg tcagcgcggc ccggtccatc ctggtcagct gcggcgcggc
     5341 ggcggtgctg gccgtgtggg agcagggcag gccgctggca gaggtggcct ggagctgcgt
     5401 ggccggatat gtggtggagc agccggagct gacgggcatt ttgtccggcg gcggcctgtt
     5461 gtccatggtg aatggaatgt gcatcgtgct gctctcctgc gcctcctccg ggatcctgaa
     5521 cggggcggag ctactggagc cggtgaaggc gcggctggag cgcatggccc ggaggaccgg
     5581 cctgtatgtg actacggcgg cggtgtctgt ggcctccgcg gccctgctgt gcaaccagtc
     5641 tatcgccctg gtgctcaccg gacagatgat gggagagagc ttccgccgca gcgggaagga
     5701 cggccttctg ctgtcccagg ccctggggaa tacggccatc gtgctggcgg cggtggtgcc
     5761 ctggtccatc gccggcagcg tgcccctgga ggccatgggg gcctccgccc tggccttccc
     5821 cttctccttc ttcctgttcg ccgctccggt gtgcgactgg ctctgccacc ggaagcagtt
     5881 ccaaatggaa taaagttcga tccgttttga aaaagcggct gcggcctgtt cggccgtctt
     5941 gcctccggcc ccgcggggcc ggaggctttt ctgctctttt gcctgaactt tctctctgtt
     6001 atgctagcgt tgcgcgggcc ttctgggcct tccatcccaa agagagaaat caaggaggag
     6061 accaccatgg caagaaaagc agtagaacgg aatatatcct atgacgatgt gcggcagctt
     6121 tactatgtca gcatggagct gggccgggac gagaacggga agcggatcaa gcagtaccgg
     6181 acctttccca cgctgacggc cgcccggaac gggctgaggg actttcacac ccagcgggag
     6241 cggcaggcgc gggagccaca ggatgaggtc cgggaggaga tggacctggc ccactggctg
     6301 gagtactgga tggagtccat cgtccggccc aaccgggcgg agaccaccgt gtacgcctat
     6361 cagaagatca tcgacaacca catcctgcct gccctgggtg ggattcccat gagccgcctg
     6421 tcagccaagc ggatccagcg gtactacacg gacacccagc aggcggccgg gctgtcctcc
     6481 aacaccatgc ggcggcacca cgaccttctc tccagcgccc tgcgctccgc ggtgcggcag
     6541 gatatgcttc tggcctcgcc catggaccgg gtggagccgc cgcggtcccg ggtcaaggag
     6601 gccttctttt atgacaatga ggaactgaaa cagctctatc agcttatcga ggggcacatt
     6661 ctggagctgg cggtgaagat ggcgggcagc ctggggatgc gccgggagga gatctgcggc
     6721 ctgaagtggg agaacgtgga cttccgccgc cgcctggtcc tggtgcggga ggcccgcacc
     6781 gcctatgggg ccaccatcgt ccagaaggag accaagaacc gggcctcggt gcgcaccctc
     6841 tacctcccgg aggaggtcct cctcctgctg gggcgggagc aggagcggca ggaagaggaa
     6901 cgggcccggc agggcgctgt cttcaaccgg acggaccatg tgatcctgga tcacaagggg
     6961 gcgccctact ccccaaacgc cctctccctg gccttcaccc ggtttgtgcg gaaaaacggc
     7021 ctgccccgcc tcaccttcca cggcctgcgg cacacctttg ccaccatcgc ctcctgtcag
     7081 ggggcctccc tgttcgacat cgggaaggcc ctgggccact ccacccccgc caccacgggg
     7141 cggatctata cccatctggt ggaccgcacc cacgaggacc tggtgctccg ggtgtccgac
     7201 gccctgaaat gagggggact cttttccccg gaggcgcttg taatttgccg ccgtttcttg
     7261 tatagtgata gcagaaagaa gcagagagga ggcggatgtg tgcggatcat ggccattgac
     7321 tacggcgacg cccacaccgg ggtggcggtc tccgacccca ccggattttt gacgggggat
     7381 gtgaccacca tccacagccg gaaggcggag gtggtgctgg aggagctggt caagctggtc
     7441 cagacctatc aggtggacga gctggtgatg ggctttcccc ggaatatgga cgggacagag
     7501 ggcccccggg cggagctgta ccggaccttt gccggccggg tggaggaggc caccggcctg
     7561 aagccggtgc tgtgggatga gcgccggacc acggtggacg cccaccggat cctgtttgac
     7621 gcggggaaga acggaaagaa gcggaaaaag acagtggatg cggtggcggc ctccctgatc
     7681 ctggagggct atctggagtt ccggcggatg cggggctgag ccgccgcggc ggcggaagga
     7741 aaaggagtgt agaagcaatg atgctgtttc aagtgaccta taccatgctt cccggacaga
     7801 gggatgtgtt tttgcagatg atgcgggagt ccgggatcct ggaccagatc cgccgggagg
     7861 aggggtgcct ggactaccgc tactacctcc cggaggagga ggacggcacc ctgctcctgg
     7921 tggagcggtg gaccgggccg gaggcccaga aggcccacat ggccaccagc cacatggccc
     7981 ggctgggcca ggtcaaggcc cagtatgtgg cggagaccag cgtggtcacc tgggcgctgg
     8041 actgagcgcg gaccgggaga atatgcagta aagtgaaaaa ccttttcaga agttctgccc
     8101 cggatggggt ggaacttcca cagcacagag aacggaacga cacagaggag gaacggacat
     8161 gaacatcaaa acggtacacg ccgtctattt cagcggcacc gggaccacgg agaaggtggt
     8221 caccaccctg gcgaaggatc tggcggagcg gctgggggcc gggtatgagg cctgctgctt
     8281 caacctgccc gaggcccgga agcaggccct gtccttcggc ccggaggatc tggcggtggt
     8341 gggcaccccg gtctatgccg gccgggtgcc caatctgctg ctgccctaca ttcgggatat
     8401 gatccatggg gaggggaccc tggccgtccc ggtggtgctg tacggcaacc gcaactatga
     8461 cgacggactg atggagctgc gcaacgtcct gcgggacaac ggcttctgcc ccgtgtccgc
     8521 cggggccttt gtgggcgagc attccttctc ccgtattctg ggcgcgggcc gtccggacgg
     8581 ggaggatatg gccctggtgg gtgagcttgg cgagaaaaca gctgacaagg tgagaggctt
     8641 gacagagctt cccggagagg cggtccatgt gaacggctgc gaccccatcc ggccctacta
     8701 caccccccgg gaccgccacg gcgagcccat caaggacttc ctgaaggcta agccggtcac
     8761 cgacccggac aagtgcgtga agtgcggcct gtgtgcccgc ctgtgcccca tgggctccat
     8821 cgatcccgcc gatgtgtcca acgtggccgg caagtgcatc aagtgctgcg cctgcgtgaa
     8881 gaagtgtccc accggggcca agtactttga ccacgagggg tatctctacc accagcatga
     8941 gctggaggac cagtatgccg gacggcgggc ccccagcgag atctttgtct gaggcggagc
     9001 gggcggctga ggccgccgcc cgggaggagc tttgccaggt gtgccgtctg ctgtaccagc
     9061 gggggtacac tgtgggccac gacgggaacg tgtccctccg gctgggggag gaccagatcc
     9121 tggtcacccc ctccggggtg ggcaaggggc ggctgcgtcc cgggatgctg gtgaggtgcg
     9181 ccctgtccgg ggaggtgctg gagggggaaa ggcacccctc ctccgagata cctatgcacc
     9241 tgctggtcta ccgggagcgc cccgatgtcc gcgccgtggt ccacgcccat ccccccatgg
     9301 ccaccgcctt cgccgtctgc cgccgcccgc tgagggagcg gtacctcacc gagaccatca
     9361 gcggcctggg agaggttccg gtggccccct ttgccctccc atccacccag gaggtgccgg
     9421 acagcgtccg cccctttgtg cgggaccaca gcgcggtgct gctggccaac cacggggccc
     9481 tgacctgggg gccggatctc tgttccgcct ttgaccggat ggagacggtg gagcagacag
     9541 ccaggatcta tgccaacgtc cagctgctgg ggggcggtgt ggagctgacg gaggcccagg
     9601 agacggccct gctgggtctg gcgggccggt atgcggtcct ggcggggccc agaacagagg
     9661 aggagagcgt atgacggcac agagccagcc ggagaatgtc cgcctgggag aggacgggga
     9721 gagcgtggtc ctgctggacc agacgcggtt gcccggccgg gtggagtacc gcacggtgcg
     9781 ggacctgaag gagatggtgg aggccattcg gaccctccgg gtgcggggcg cgcccgccat
     9841 cggcgtcttt gctgggttct gcctgtccgc cctggcccgt caaatggagc gggcggggct
     9901 ggataccccc ggacttttga gagaattaga ccgaaaaggg aaaatacttg tcagctcacg
     9961 ccctacggcg gtcaatctgt cctgggcagt ggagcgcatg atggaccgcg cctccggact
    10021 ggccggaggc gccccggagg agctggccgc cgctttggag gcggagagcc gggccatccg
    10081 ccgggaggat gaggagatgt gccgcgccat tgcggagcac gggctctccc tgctgaaggc
    10141 gggggacggg gtgctgaccc actgcaacgc ggggcctctg gccacctccc gatacggcac
    10201 cgccctgggt cccatcctgc tggccgggga acgggggatc cccctgcggg tgttcgccga
    10261 cgagacccgg cccctgctcc agggggcgcg gctcaccgcc tgggagctgc atcaggcggg
    10321 ggtggacgtc accctgatct gtgacaacat ggcctctatg gtgatgaaga acggatgggt
    10381 ccaggcctgc tttgtaggct gcgaccgggt ggcggccaac ggcgacgccg ccaacaagat
    10441 cggcaccagc ggagtggcca tcctggccaa acactacggg atccccttct atgtgctggg
    10501 gcccacctcc accatcgacc tgaactgtcc cgacggggac cacatcccca tcgagcagcg
    10561 ggatccggag gagatccgct ccctgtggta ccgggagccc atggcccccg ccggggtgaa
    10621 gtgcttcaat ccggcctttg acgtcaccca ccacagcctg atctccggca tcatcacgga
    10681 aaaggggatc tgccgcccgc cgtttcccca gtccctggcc gcgctgtttt ccaatggggc
    10741 gcgctgagtt cccaaacacg caaaaacaca caaaaaatgt ccctctgcca tggcaggggg
    10801 acattttttg atcttaccgc tccatcttcc agaaaaacgc gctgtacggg ggcagctggc
    10861 tcccgccgcc gaagtcgtgc tgctggccgg agatcaggtc ccaggccagg ccgcagccgg
    10921 cgtcgaagtg ggcagtatag ggctggctgt ccgcgttgac cgccaccagc acccgctccg
    10981 tctctgttct gcgctcgaaa atgagctgta ggttggtgag gagcacattc cgatagctgc
    11041 cctcccgcag ggccgggctg ccccttcggg cggcggccag ccgggcgatc cagcttgtca
    11101 gggcgttcca ctccggcttt tccagggcgg ggcggagacc ggcgtcactg cacggggctt
    11161 tttcgccgga gatgccccac tcgctgccgt agtacagggc gggggtcccg ggcatcccga
    11221 acagaagggc gtagatcagg gggaggtggg cggggtcccg gagcttggag gcgatgcggg
    11281 tgacatcgtg gttgtccacg aaggacagca ggtgggcccc cttgtagagg gtccagggct
    11341 ccggcccgta ctggcgggcc agactgtggc cgatctcgaa caggttgcgg tcgttgaggc
    11401 tggaccacag gcccttatag cactcgtagt tggtgaccga gtggcacagc tccggcccca
    11461 tccagaggtt gtagtccccg cccaggacct cgcccaccag aacaaagccg ggcttcagcg
    11521 tctgggtgaa ggcccgcagg cgcttcagat agctggcggg gaggcagtag gccacatcca
    11581 gccgcagccc gtcgatcccg aactgctcca cccagctccg gatggcctgg aactggtact
    11641 ccaccacatc cgggttgtga aggttcagct tgaccagctc gccgtggccc tcccagttct
    11701 cataccagaa gccgtcgccc cggtcggtgt cgccgtcgaa gttcagatag aaccagtccc
    11761 ggtaggggct gttccacttc ttctcctgta cgtcccgaaa ggcccagaag ccccggccca
    11821 catggtggaa cactccgtcc agcaggaccc ggagcccggc gtcgtgaaag gcgcggcaca
    11881 cctgggccag gtcttcgttg gtccccaggc gggggtccag ccggttgtag tcccgggtgt
    11941 cgtagccgtg gcggtcgctg tcaaacaccg gattgagcag gacggtgtcc gcccccagct
    12001 gtttgatgtg ctccacccag tccagcagcc gcaggatgcg gtgctcggta tgcccgtcgt
    12061 tgtccgccgg cgcgccgcac aggcccaggg gatagatctg atagaggata gcttggtcaa
    12121 accacatatt ggacactcct ttcgtctccc ggccgggtcg ctccggcgga gaggcgcgtt
    12181 gatcgcaggg cgagggaaaa agacagggcg ggccccgccg ggtccaccct gtgaaacggc
    12241 ggcagagacg ccgccgccgg gtatgccgtg tgacataccg acattataca gtttttccct
    12301 gaaaaatcaa gaaaaaggtg gtgggatcgg aggggtcact gcctggtcag ataggggagg
    12361 accagggcag ccagcgcgat caccagcagc aggatgcggt tgaagcggtc cataggcggt
    12421 ctcctttctg gaactttgat ccagatacag gatagcacga aacaatacga ctgtcaaatt
    12481 gaataaagag aagcgagaaa gaccggacaa aatagtgaaa aagacggatc agacctgacc
    12541 ctttgcaagg cggagcgggg gcccgtccaa acaaatcacc tgatcggcca gggagcaggc
    12601 cagggtctcg tggctggaca ggaggaccgg ggcgtccagg gcacggaggg cgtccatcag
    12661 gcggcggacc cgggcggggt ccaccccggt gaagggctcg tccagcagat agagctgtcc
    12721 gcccagggcc agggcccgga ccagggccag gcggcggcgc attccgccgg acagggaggc
    12781 gggaaagctg ccctcctcgc cggtgagctc ggccagggcc agccaccggg ggacctccgc
    12841 ccagcgggac tgggggagca catccatcag gtgctgctcc accgtgcgcc aggggaacag
    12901 gcggtcctcc tgaaagagaa gggaagtctc acggggaggc ggggcctgga tccggccgct
    12961 ccgggggcgc tccagtccgg ccagacagcg cagcagggtg gtcttgccgc agccggaggg
    13021 tcctgacagg acggtgaccc cccgctccgg cagggtgagg gagaagtcct ccagcacccg
    13081 cttctccccg taggagaggg tgaggtgaga gacggagatc acagcggctt ccccccctct
    13141 ggacggcgga tgagccgggt cagcgcccgc tccatgagca ggctgagcag gatgaccacc
    13201 gccgtccagg cgaagaggtc cgcagtttcg aaggtctgct tggcccggct gatctgggcg
    13261 ccgatgccca gccgggggag gcacaggacc tcggcggcca cgccggattt ccaggccagc
    13321 cccatggcgg tggcagcggc ggagcgcagg tggggccgca gggaggggag gtacaccagg
    13381 cggaccgttt tccagcggga gaagcggtag gcccgggcca gctccagcag ctgtccgtcg
    13441 gtggcgcgga tcccctgggt caggttctcc cacaccaccg ggagcaccat cagtccggca
    13501 atcaccgcag gcacctggtc ccggccggtc cacagcagca ccagcaggat gaaggagacc
    13561 accggcgtgg cccggacgat ccgaatgact ggggcgaaca ggcggcggca ccaggggctg
    13621 gtacaggtga gggcggccag cagaccgccc aggaccgcgc cgcccgccat ccccgctgcg
    13681 atgcgggcca gagaggcgag agcggtccac cagaattccg gggaccgggc caggcggaac
    13741 agggccagca gcaccgtggc gggatagggg aggagcagct cgttgccccg gccctccaca
    13801 tggagctcca ccagccaggc tccaagctgc cagaccccca gccagaaggc cgggggcagc
    13861 agcagggaca gggcgcggtc caggcggtca ggacacgcca tagtagaagg agtcataggg
    13921 gagcacgccg ccgatggagg cggggtcggc gtcatagagg acctggtaat agtcctccag
    13981 caccgccttc atctccaccc cggtgaggca caccagattg cactggggga tggcggatct
    14041 ggcgatggcg gcgttggggg tgatgccgta ctgggccacc agctgggcgg cgtcttccag
    14101 gttggcgggg tcggacatat aggcgatgga gtcctgatag gcgttcagga agttctccac
    14161 cccctgggga ttctcctcaa tatagtcggt gcgggccaca atgcagccca tggccagctg
    14221 gccgctgctc accttggacc actcctcggt gagggacagg gcctgccgca ccccggcatc
    14281 ctggagcatc agggcggtag cggcggggac ggggagcaga cagacgccgt cctcagagga
    14341 ggccatcttg gcggtgacct cctgggcggt catccactgg atgtccacat tggcggggtc
    14401 caccccgttc tcggtgagca ggtagttgag gacaaactcc gggttggccc cctggccggt
    14461 ggcatagacg gtcctgccct ccaggtcggc catggactgg acggagttcc ccttctccag
    14521 gatgtagagc acccccaggg tgttcaccgc cagcacctgg acggccccct cggacttggc
    14581 ggacaggctg gcggccacgt tggtggccac ggcggcgatg tccacgtccc cgttgatgag
    14641 ggcgctggag accgcctggt tgtccgcctc cacggtggcg gtgttgatca ctggagacgc
    14701 ctcgccgttg gcgacggcct cgggggtgac agcttcgttg tccgccatca gcttggcagc
    14761 tcccacgccg gtggggccgg acaggaccat gacattgggc agcacatagc tgtcagagga
    14821 ggaggcccca gagctaacag agccggactg ggagtcggag gcgcttccgg aggcggagga
    14881 ggagtccttc ccaccgccgc atccggccag ggcggacacc atcagggcgc cgcacaggag
    14941 cagagacagt tgcttgttca gtttcatttg aatcttcctt tccaatgttg tgtttccggg
    15001 ctgtccgggg tcttacagca cgccctccag ccccgccggg tccagaacgt acatggactt
    15061 gccctcccgg cggatgagcc gcgcgtcctc cagggcctgg aaggcccggt acagggaggc
    15121 gcggctgatg cccaggcgct tggccagatc ggtggcggac aggcgcagcg gttcgccgga
    15181 gggaacggtc agcaggtatc gggccagctt cccctccgcg ccggtctggg ccaccgagcg
    15241 cagccgtccg gacaggaacc ggatgcgccc ggtgagatac tggaggtagt tgcggcggag
    15301 gaggggctcc tcccccagca ggcggtccag ggtctcctgg gggagaaaca ggacccggca
    15361 gggggactgg gccgtgagga cggcggcgaa ctccggcgcg tcgttgtaga gggtggccgc
    15421 gccgaagagt tccccggccc gcagcaggct gacggacagg gagccgttgg tcacctggac
    15481 ctgcccggag aggatcacgc ccaggctctg ccggaactgg ccggggccgt agagcacctc
    15541 cccggcggcg aagggctcca ccgtgacgcc gggcagggcg ggcagggagc ggagcagagc
    15601 gtcctccacc cccaggaaca ggggacagcg gctcagaagg gcgcgctcct ggtcagagag
    15661 atccggggcc atgggcgggc cctcctttct gaaaaagtgt cttatttgag acagattgaa
    15721 tataccacgg acaggcgcaa aggtcaaccc cggacatagg accataggat atctgcccat
    15781 cttccagcac gcgtctgaca aaaacagaaa agaagaactg ccaaagcaat ggcagttctt
    15841 ctttgaaagg aagggttagt tcagcgacag cagatattcg cccagctcgg ccacggagga
    15901 gaccagcctc catggcgata cctgacccag ctctttctca gaaccatagc cataggtgac
    15961 cgcaacacat ggaattccag ccttctgggc accaaggaca tcgtgctcct tgtcacccac
    16021 cattatggct cgatcccgct ggtcggacag gcccagccgt ttcagtgctt cctcaataac
    16081 atctgccttt tcagtccgct tttcatccat ggttgctccc acaatctcgt gaaaaaaagg
    16141 cgtcagccca aaatgttcca ggatctgtcg gacgtagtgt tccggtttag aggaagccac
    16201 tgacaaaaca tatccccggt cggtcagggt ttggagcatc tctgggatgc cggggtaggg
    16261 tctgttttca aagacccctg tggtggaata gcgctcccgg tagaattgaa ctgcctggcc
    16321 ggcttcctcc cgggtcatgc cggaaaagga catgaactgg gggatcaggg gcggcccgat
    16381 gaaggatcga agcttttgaa catccgtctc tggctttccc attttctcca gagcatactg
    16441 gacagagcgg atgatcccct caccggactc cgtgagcgtc ccatccaaat caaataaaag
    16501 ataccggctc atgctgttcc tcctatgtca taacatcaag cgtgatcatt atatcatcgg
    16561 atgggggtgg gttcaatcaa aaaatccggc aggtctggga cacggcgctc tggggagcgg
    16621 cataggatgg ggggagatca tacagggagg gatggagatg accgatcact gtggaccggg
    16681 gaggcggaag acccaggccg gccggcgttc agacccggag gagatgctgg ccaaggtgca
    16741 ggccgccgcg gaggagatga ccatgcggcg ctatgtccat atgccgccgg aggggcggcc
    16801 ctgctgtccc gagggctgcc tggcggcgct cctgggggag ctgaaccaga tccaggacca
    16861 ccagaaccag atcctggcgg agctgctgcg cgccctggag cggatggccg acgcactgga
    16921 gaggcggtaa ccgaagtcca ggcgcagaaa gatgagacgg tcgaaaaggc tttttcgaca
    16981 agctgaaatc ccgtctgcca cggtatggca ggcgggattt tttgagggat ggagttacgg
    17041 ctgatccgcg tccagacggg tcttgaagta gctgtcggcg cagtagatgg cggccacggg
    17101 gttgttgccg gtggcccggt ccttggccac cagggtggtg accggggcct gggaatagcg
    17161 gtagaacagg gagtcgtggc ccacgcacag ccccaggcag atgttgaact gggtctccac
    17221 ccggttgagg agctccgcct gggcgatggg gttgcacatc acctcatagg tcccggggcg
    17281 gacccggtac tccgggggca cccccgcccg gtccttgggg atggagcctg ttttacagat
    17341 gacggaggcc acctggaagc cgttttggcg caggacccgg tccaccacct cggcctcccg
    17401 gtgcaggccg gcgcagaagg ccagtcccag ccgggtatag cccatgcgct tggaaaactc
    17461 cactacctcc cgcaggcggg gccagcggca gtagccgatg gcctcggtgg cggcgctggc
    17521 gatgaaaaaa tcgtggttct ccggcttgaa atactcctct agaatggagt caaagaaggc
    17581 acggtcccgc atgggacagt ttttcggcat cttgtccagc tgtccggagc ggcaggcctc
    17641 aatggtgcag gacgcgcagg taaacatggg gtcttcctcc tttacagatg taaacagatc
    17701 gggggagggc gtcacaggct ggacagatag gccgccggac cgctctcgta caggtcgccg
    17761 ccgtgggcgt ccaggatcac cgtcaggggc aggtcccgca ccgtcaggcg gcgcaccgcc
    17821 tcacagccca ggtcctccca gcagatgacc tccagctcct ccacgctggc ggccatcagg
    17881 gccccggcgc ctcccagggc ggccagatag actgcgccgc tgcggaccac cgcctccttc
    17941 accgcctggt tccgggcccc cttgccgatc ataatggcct gccccaggtc cagcaggcgg
    18001 ggggagtagg cgtccatccg gccgctggtg gtgggcccgg cggagccgat gatctgtccg
    18061 gggcgctccg gcgtggggcc cacatagtaa acggcggagc cctccacggg gaaggggaga
    18121 gcctctcccg catccagaag ctccatcatc cgcttgtgtg cggcgtcacg ggcggtgtac
    18181 accgtgccgg agaggagcac ggtgtccccg gcccgcaggg gggccagatc ctccttggct
    18241 acgggggtgg tcagtttgta ttgcatggaa acagcctcct aatcgtttgg atggatatac
    18301 agggtcataa gtgctccaac agaaagcggt ggatcgtgtc cgcaagctcc ttggggcggc
    18361 ggcggtacag atcgtgaggg agatcaaagg tccgcaggga tgagtccgga agggcggaga
    18421 ggtaatgctg tccgcacgca cgccaggaat ctccggccag aggagtgccc tttccgttgg
    18481 agaggagaaa cagggtggga tgggagaggg gaggcccttc ctgctccacc tgccggacag
    18541 agcggggcag atccctggcc tcattgaccc acacggcact agcaaagttc cggcgggcgt
    18601 caaccatcag ccgcctacgc tctgcggcgg gacagtggcc caggagggcg ggagcgagca
    18661 gccagaagag tagggtacgc ttccagcctg agcggttgag ggcctccagt cggctcagaa
    18721 tggcctccag atccatgtgc tgatagacct ccggtacagc ggggtccagt cccactaggg
    18781 cggagacctc ctccggatat ttgaacgccc agtgaagggc ctccaggaag cccatgctat
    18841 gggcggccag cacgacggga acaggaatac cgcaggcgga cagcaccctg cggtattcct
    18901 ccaccactgt gtccacatct ctgggagcct ctgtcaggtc gctatgtcca tagccaaatt
    18961 tagaggggat gaggaccgag taccacgggc tcagggcgtc cgccagcgga gcgtactcca
    19021 ggctgggaaa caggatgttg gagccggaca ggatgagcag ggtgaccgga ccggagcctg
    19081 tatccgatac atgaagcttc cggccgtcca cggcgatcat tttttcttcc acggcagtac
    19141 ctcccgggga gtgctcacag ctccgccgcg gcccggcggg tgacgtggca gctgacgttc
    19201 accgccacgg gcagtccggc cacatgggtg ggggcggtct cgatggacag gcccaggcag
    19261 gtggttttcc cgccgaagcc ctggggcccg acgcccaggg cgttgatctc gtccagcagc
    19321 tccgtctcca aagcggcgta gaaggggtcg ggatttcttt gatcgatggg ccggagcagg
    19381 gcgtgttttg ccagataggc tgccttgtcg aaggagccgc cgatcccgac gcccagcact
    19441 gtagggggac aggggttgga gcccgccagg cgcaccgtct ccaccacgaa gcgctttacg
    19501 ccctccaccc cgtcggcggg cttgagcatc cccaggcggc tcatgttctc actgccgaag
    19561 cccttggggg ccacggtgat tttgcaggcg tccccgggga ccagccggag ggtgatggcg
    19621 gcgggggtgt tgtccccggt gtttacccgg cggagggggt cgcccacgat ggatttgcgc
    19681 agatagccct cgccgtagcc ctggcggacc ccctcatgga tggccgcctc gaactcgccc
    19741 tcgatgtgga tctcctggcc cagctccacg aacacacagg ccatgcccgt gtcctggcag
    19801 acgggcaggt ttttttcctc cgccagttcc aggttgttcc acagcaggcc cagggtctcc
    19861 ctggccaggg gccagggctc gctctgccgg gcggcgtcca gggcggccac gatgtccccg
    19921 ggcagatggg tgttggcctg gatgcacagc ttggccaccg tctcggtgat ggcggcggcc
    19981 tggatggtac gcatagacac tccctctttc tctttgggtt tgttccattg tatcaaaaac
    20041 ccggcggatt ttccagagcg gcgcagaaaa atcccggcgg gagggggacc tcccgccggg
    20101 gatgcgcttc cggctgctca gggatagaag ttgaaggggg cgtagacaga ccggatgggg
    20161 ccgtatgctc cggcggagcg gctccctccc aggtcccgga tggagacgaa ggggtagtgg
    20221 agccagcgct cttgatggcg gagaaagacg taccagaagt cgccgtagga ccagtcggat
    20281 ggatcctcca ccagggggga cgggaccgcg gcggtggtgt aggcgatcca gccccgcatc
    20341 ccctccgcac cccgggggat ggagaaggag aaggaggggg agtagtgaag atggcctagg
    20401 cggtgcaact ccagctcata ccacaactgc tggtcctcgg tgagttcctc tgcggtctgg
    20461 ccgcccgcca gccggatctc cggggagaca gccagggtct ggccgttctt ttcccacagc
    20521 agccgcccgg cgtagtcctc cgcgtggaaa tttccggacg gatccacgga gaactgctcc
    20581 tgcaaatgga gatcgggcat ggcccggtag cggaagaaat ggatctgccg cacctggtcc
    20641 tccggcagga agaggagcca ggaggacagc tccgcctccc cgccgcccaa ttcatggagg
    20701 acggcggggc cgtcggtgcg aggcgtctcg ttgtccgtgt ggtcagtgtc ataggtccgg
    20761 ccggccctga ctgcctctgc ttgggcgcac cgctccagtt ccgccccgtc cagggcggcg
    20821 gcgacgtcct ccggaaggcc cagggagacc aagcgggccc ggatgtcctc ctgctccgcc
    20881 gtcagctggg ccgccggctc cgccgggccg gtggggatgc ggccgcccag ccacagggcg
    20941 ggggggatgg cggccagggt gagggcgaac acccccagca ggaagggccg ggcggacacc
    21001 cgcacaggtg cgggggcgat gtcatagccc aggcccgcca gggcgtcgct ctggtgggcg
    21061 atgcacacca gcaggcggat ctccaggatc aggaaagcga tgggccggag gtaatagagc
    21121 cagtggttgg tgatggtgac tcccagcaag ctgggctcag tggcggggac cagctcggac
    21181 cacagggcgg tggccagggc cagcagatag cagagaaagc cctccctcag ccagtccctg
    21241 gggggctgag cctctcccac ggtgcggaag gcggcccggg agccccgcca cagggcgaac
    21301 aggaggagca ggtccatccc ccggatgggc caggtgagcc cccagttgac ggcgggattt
    21361 cccgcgatcc accccatcac cggggtggcg gccagaatgt ccagggcgat atggatggcc
    21421 agcagcagac cggacagccc ccagcacagc cggaaccagg gattttcccg ccgcagggag
    21481 cggtacccca gatacatcag agcggccccc aacaggggaa gcaggtactg gagatagaaa
    21541 aattccagcc ggaaggtggt cagggccatc ccccacagga tcctggacat ggcggcctgc
    21601 caggggttgt agtcctggac ctcgccgggg tcagggggga gatcggccat ctcctgggtg
    21661 agacggagat caaattcgtc ctgcttcaca gaagatcacc tcccagcttg ttctggagtc
    21721 cctttcggat gcggtacagg cggccctcca ccgcccgcgg ggtgagcccc agctccgccg
    21781 caatgcgggc ggtggactgg agatagtaat atttgcggta gaacagggcg cggtcccggc
    21841 tggacaggga ggccagggca cgctccagac gctccgcccg ctccttccgc agcagctgct
    21901 cctccggacc cggggcgggg tccggacggg tctcatccag cgcctcggcg ctgtggcgct
    21961 gtgcgctcct caggtggtcc acggcggcgt tccggcacag cacggtgagc caggtagcgg
    22021 ggctggagcg ctccgggtcg tactgctccc gcttttccca cagcttcagg cgcacctcgg
    22081 ccaggcagtc ctcctgccgg tgggggtcgg acagcacccc agacacgatg taccgcagca
    22141 tcccctcata gtgggccagc aggggctcca gggcctcccg gtccccctgc tggagtcgtt
    22201 ccatccacgc ggacgggtcc atgccggtca cctccttctg tcnnnnnnnn nngggggaga
    22261 acccacggaa aaatctgccc ccggtctgtt tgttcccagt atagcagggc gggggcggcc
    22321 tggaaaggaa aatataaagg tgggctttgt gaccctgctg gccaaggata cttggggggt
    22381 ctggcacaag cagatctacc tgtatcggat ccggtaggac ggaggctttg agggcagcga
    22441 cggctgtctc accctgtccc ggcccccaga cctcaccggc tttcagcagg ggttctggtg
    22501 acgggacgca gggcgttttg cgcaggggcg gacgaaaaac gcggatctca gcctgcggga
    22561 gaaacagaaa aacagtgacc gggagccgtg tggctcccgg tcactgcttc gctgcggtac
    22621 aaacggcagg tgcggcgtat tgggggcttt gccccgcgct ccggcccgtt tggctccgcc
    22681 tcttgtaaaa aagccggaaa tgtgagacaa tagggccagc ctaacaaaga caggagacac
    22741 aagtatgatg accgacctgt ttgaaaagtc catcgccacc ctggaacttc cccgggtgct
    22801 ggagcagctg gccgcctgcg cctccaccca ggaggggaag gagcgctgcc tggccctgcg
    22861 gcccatgacc gacccggacg atgtgcagcg ggcccaggag gagacctccg ccgcggtgga
    22921 gatgctcatt aagcggggga gccccggctt ctccggcgtc aagcccgtgg gggccagcct
    22981 ccaccgggcg gatatggggg gcagcctgaa cacccgggag ctgctggagg tggccgccgt
    23041 gctgcggtgc gctcgcaccg tccaggacta cggggccggg gaggaaaaga cccccatctc
    23101 ccacctgttc cgcgccctca cccccaaccg cttcctggag gagaccatca ccaactccat
    23161 catcggggag gacgagctgg ccgactccgc cagcagcgag ctggcctcca tccgccgcca
    23221 catccgggcc acggaggcca aggtccggga tattttgcag aggatcctcg cctccaacca
    23281 gtccaagtac ctccaggagt ccatcatcac catccgctcc gaccgctatg tggtgccggt
    23341 gaagtcggag ttcaaaaacg ccatccccgg cctggtccac gacgtgtcct cctccggctc
    23401 caccttcttc atcgagccta tgggcgtggt gaaggccaac aacgagctgc gggagctgat
    23461 ggcccaggag aaaaaggaga tcgagcgcat cctggcggag ctgtccgccc agtgcgccgc
    23521 ccaccgggag gacatcgcgg aggactatga cctgctggtg tggctggacg ccatctttgc
    23581 ccgggggcgg ctgtccctga acatggaggc cgcccagccc cggctgtccg accggtattt
    23641 acgcctgcgg aaggcgcggc accccctgct ggaccggaag aaggcggtgg ccaacgactt
    23701 ggagctgggg gaccgcttcg acaccctgat gatcaccgga cccaacaccg gcggcaagac
    23761 ggtgacctta aagaccatcg gcctgctgac cctcatggcc cagtgcggcc tgcacatccc
    23821 caccgggtcg gactccacgg tgcggatctt tgaccgggtg ctggccgaca ttggggatga
    23881 gcagtccatc gcccagtccc tgtctacctt ctcctcccac atgaccaaca tcgtgggcat
    23941 tttgcaggag gcggacgaca ggaccctgat cctgttcgac gagctggggg cgggcaccga
    24001 cccggtggag ggtgcggccc tggccgccgc catcatcgag tccgcccggg ggatcggcgc
    24061 tctggtggcc gccaccaccc actacgcgga attaaaggta tacgccatga ccaccgccgg
    24121 ggtggaaaac gcctcctgcg agttcaacgt ggagaccctg gcccccacct accggctgat
    24181 cctgggcatc ccgggaaaat ccaacgcctt tgccatctcc cgccggctgg gcctgcccga
    24241 gtatatcatc gaaaaggccg ccgcccggct ggatgcggag aacgtgcgct ttgaggatgt
    24301 gctcaccaag ctggaccagc agcgccagga gatggagaag gagcgcaccg aggcccgccg
    24361 cctgaagctg gagatggagc agtctgccgc caaggcccgg gagtaccggg agcgcctgga
    24421 ggccgagcgg gccaaggtgg tggagaaggc ccaggcggag gcccgttcca tcatccagga
    24481 ggcccgggcg gccagcgacc tggccatctc cgagctgaag gagctgaaaa agcggaagga
    24541 cctggactgg cagcaggtga acgacggccg ggcggaggcc cgccgcctgc tcaacgaggc
    24601 cgagcggggc atcggcggcc ccgtgcagga gccggaggcc ccccctccca cccgggaggc
    24661 ccgg
//
LOCUS       DBMDSJ010000002        16671 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_1312_length_16671_cov_2.446409, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000002 DBMDSJ010000000
VERSION     DBMDSJ010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 16671)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16671)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16671
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1312_length_16671_cov_2.446409"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(132..1421)
                     /gene="guaA"
                     /locus_tag="ACZPAQ_00135"
     CDS             complement(132..1421)
                     /gene="guaA"
                     /locus_tag="ACZPAQ_00135"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR0902410.1"
                     /translation="MKQDMILILDLGSEENPRLAREIRALGVYSEIHPHDITAEELAA
                     LPNVKGIILNGGPNRVVDGVEIDAAKEIYNAPVPVLLADHKGDQPWPEDAAARKEALS
                     NFVFQVCGAQANWNMENFIADQVELIRQQVGDKKVLLALSGGVDSSVVAALLIKAIGK
                     QLTCVHVNHGLLRKGEPEQVIKVFRDEMDANLIYVDAVDRFLDKLAGVSDPERKRKII
                     GAEFIRVFEEEARKLEGIKFLGQGTIYPDIIESGTKTVKAVKSHHNVGGLPEDLDFEL
                     VEPLKMLFKDEVRACGKALGLPDSMVYRQPFPGPGLGVRCLGAITRDRLEAVRESDAI
                     LREEFAKNGLEGKVWQYFTAIPDIKSVGVRDNKRADEWCVIIRAVNTVDAMTATVENV
                     PFDLLQHITARITAEVKGVNRVLFDLTPKPTGTIEWE"
     gene            complement(1938..5147)
                     /gene="carB"
                     /locus_tag="ACZPAQ_00140"
     CDS             complement(1938..5147)
                     /gene="carB"
                     /locus_tag="ACZPAQ_00140"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR0902411.1"
                     /translation="MPKYTDIKKVLVLGSGPIVIGQAAEFDYAGTQACRALKEEGLEV
                     VLINSNPATIMTDKDIADHVYIEPMNLPSITQVIEKERPDALLPNMGGQTGLNLAMAL
                     YENGTLEKYGVRLLGTSPASIHKAEDRQGFKDCMESIGQPCVTSKVVNTVEDAVSFTN
                     EIGYPVIVRPAYTLGGTGGGIAYDEVSLREISDRGLNLSRVHEVLIERCIAGWKEIEF
                     EVMRDSAGNVITICSMENIDPVGVHTGDSIVVAPTQTLANREFQMLRSAALDIISALE
                     IEGGCNVQFALNPDSYEYAVIEVNPRVSRSSALASKATGYPIAKVAAKVALGYTLDEI
                     PNAVTGKTTACFEPTIDYCVLKIPRLPFDKFTTASRTLGTQMKATGEVMAIANSFEGA
                     LMKAIRSLELNVRALKLDKLEGLYTDEIEDLLVQVTDERLFVVAEALRRGFSPQKINH
                     ITKMDIWFLDGFQRIIAMENELKSCKGEPDMDTLKRAKEMCFADSYIGALCGMTQAQV
                     KALRERYGIIPSFKMVDTCAAEFDAETPYYYSTYDGENEAVDNGSGKKKVLVLGSGPI
                     RIGQGIEFDYCSVHSVWAFRRMGYETIIINNNPETVSTDFDVADKLYFEPLTAEDVQN
                     IVELEKPWGAVVQFGGQTAIKLAKALTEMGVQILGTSADGVDAAEDRERFDEILEKCA
                     IPRAKGRTVYTTQEALQAANELGYPVLVRPSYVLGGQGMEIAYSDRNIEEFMRIINLT
                     VQEHPILIDKYLMGRELEVDGVFDGEDILIPGIMEHVERAGVHSGDSIAVYPPINLEE
                     KHRELILKHTKNMAKHLNVIGLINAQYILYNDDIYVIEVNPRSSRTIPYISKVTGVPI
                     IDLATKVMLGEKLKDLGYGTGLYREASYFAVKAPVFSFEKLTDVDTGLGPEMKSTGEV
                     LGLAETYPQALLKAFKGAGLKVPKRGSRIIVTVKDEDKAEMVGIARGFEEMGVEIYAT
                     SGTCDALTEAGIQCKRVNRVSQAHPNILDMIQSGTVDLIINTPTKGRKQDSDGFKIRR
                     SAVEHSVACVTAIDTARAVLTVREQSRSEDLTPIDITKI"
     gene            complement(5147..6229)
                     /locus_tag="ACZPAQ_00145"
     CDS             complement(5147..6229)
                     /locus_tag="ACZPAQ_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR0902412.1"
                     /translation="MKATLILANGSVFSGTSIGGTSDRICEMVFNTSMTGYQEILTDP
                     SYAGQGVVMSYPLIGNYGVNHEDNESSRPWVEAFVVRHLSPRGSNFRCEGDLNTYLKE
                     HDITGIQGVDTRALTKILRSQGSMNGMLTCAEHFNVAEVMERLRSYEVKDTVRKVTRT
                     QPEVFEAYGEEKHLVAVMDYGVKQNIIECLRRRGCKVVALPAYTPVGEVLSGGFDGVM
                     LSNGPGDPEENTEIIAELRKLYDSTIPIFGVCMGHQLLALATGAKTGRLAYGHRGGNH
                     PVRDLEKNRVFITSQNHGYMVLSDTVDPAVAEVSHINVNDGTCEGLRYKRPNCFTTQF
                     HPEANAGPKDTEYLFNRFIDSMGGAK"
     gene            6509..7201
                     /locus_tag="ACZPAQ_00150"
     CDS             6509..7201
                     /locus_tag="ACZPAQ_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIR0902413.1"
                     /translation="MFTGLIRTIILYILIIAGIRLMGKRQVGELEPSELVLSLIIADL
                     AAVPMQDFGIPLLTGVVPILALLAMTMILSVLTMKSIRFRALLCGRPSVVIRRGEIDQ
                     GEMRRNRLTVDELLEELRSQGYTDLQKVEYAILETNGQLSVLPRAAEKPPTLGQLGLE
                     AQESGLPLVLISDGRLLERNLRVRGYDGHWLDKQLEQRGLKRWEEVFLMTVDEQGQIY
                     LAPREREGRRGA"
     gene            7198..7578
                     /locus_tag="ACZPAQ_00155"
     CDS             7198..7578
                     /locus_tag="ACZPAQ_00155"
                     /inference="COORDINATES: protein motif:HMM:NF025634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HIR0902414.1"
                     /translation="MKRLWSAAALLLVLLGGALWNAWYADRLTGRMAGQLEQAQELAD
                     RGDWEGAVRLSRESFHLWQAHDAYLHVMMRHSDTDQILRGFRALDQYLALEESDQYTA
                     ANAELICQLELLSEMEQPSLVNIF"
     gene            complement(7640..9175)
                     /locus_tag="ACZPAQ_00160"
     CDS             complement(7640..9175)
                     /locus_tag="ACZPAQ_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR0902415.1"
                     /translation="MTLSMILTHLALVVAALVFAILAGVPLGILCYFYPSARRVVLRV
                     VDLIQTTPALALLGIIMVFMGAGKPTVIVGLALYSLLPIVRNTHLGLSQVPEYLIEAG
                     RGMGMTRMYRLLHVELPLAAPIIFTGIRIATVNAIGTAVFASFVGGGGLGSFITTGIR
                     QDDMEAILLGTGVLMAMALVLDLLMGLAERYLNSRTGQRSRTSRLIARAASAAACVAA
                     AALCVVAFFPQRTDGLVLYQGEFSEVQLVNSMIQQLVEERHGIPVTIKDQMTAKNNFA
                     ELSGAGHSCDLMYTWDGTLLTTIMGLDTSDVPDGQTLYEFVDQRMRGEYGLELMGKIG
                     VNNTYSIGVTQQVMDTYHPETISDLIPIAGQLRFGAEQDFFTDAGSMKFGPFSKFYGL
                     NFQDVLHVDIMMKYTMVEQGAYDVMVVYATDGLNKRANLTILKDDLHFFPDYYGTILV
                     RSDLFERFAEEAPGLKETLQLLNGQFTDEKMSELTYRVDVEGEAVDDVARDFLISAGL
                     LPA"
     gene            complement(9172..9798)
                     /locus_tag="ACZPAQ_00165"
     CDS             complement(9172..9798)
                     /locus_tag="ACZPAQ_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0902416.1"
                     /translation="MSIHDLPVKLAEHLFFTLASVAIGFALGVVLGVLLSRRPRLSGV
                     VLPILSIFNTIPGIVFIGLLFLAWGTHAATVLAALGIYATFPVLKNTYAGLVSVDPQY
                     LEAARGCGMSPWQSLLRVELPLALPTIIGGLRMSTVYTVSWAVLASMIGLGGLGDFIY
                     AGLSSNDNALILLGAIPAALLAFALGTLVDLLQRRTVPKGLRKGGSAT"
     gene            complement(9805..10917)
                     /locus_tag="ACZPAQ_00170"
     CDS             complement(9805..10917)
                     /locus_tag="ACZPAQ_00170"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR0902417.1"
                     /translation="MLRFEHVSLSYGSQQILDDLSFEIQEGQMAVLIGPSGCGKTTTL
                     KMINRLIEPTAGRIYLDGQDITTKDKVELRRHIGYVIQQIGLFPNMTVAQNISVVPTL
                     LKYSREECDRIVRELLDMVNLPYEQYAHKYPSEMSGGQQQRIGILRALAASPPIVLMD
                     EPFSALDPMTRRTLQQEVRTLQQKLNKTFVFVTHDMAEALDLADVIIFMDHGRIVQMA
                     PPEEMLSHPASGQIQDFLGKFIDLQAHKELTAADFMRTGVYTVSTRKGINECISKMQR
                     HNVDTLIVVDDQARYQGTVSIADIRLTGHVVQTIEPLIRCNTPTVHAEDNAKDCFDQL
                     ISSGAPYLVVLDGQGQVAGIITKTSMASAMAEQLWG"
     gene            11196..12440
                     /locus_tag="ACZPAQ_00175"
     CDS             11196..12440
                     /locus_tag="ACZPAQ_00175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0902418.1"
                     /translation="MSFLTAAAALLGAGITLWGSYYLLVGLMSWRRPMEYGRHPASTR
                     FAVLVPARNEELVVGSLINTLLMQDYPPELYDIWVVPNQCTDHTALAARRFGAHVLEC
                     TVPVKSKGEVLRFAWERLAHRGYDAVCVFDADNLADPQFLREMNNAYRAGARVAQGFR
                     DSKNPYDSVVSGCYSIYYWMMDRLHNGGRAGLGLPAMINGTGFMVAASVLERMGGWRT
                     ETISEDLELSVQCALEEVQVFWVPKAVTYDEQPLTFRESVKQRRRWTSGTLQVAGSYL
                     PAVGRALAARPSGATWDVGATLLIPAYQMAALVNLAVSALAAGQMGPDGFSPLLCAAG
                     AAGSLAIAALGATLLAALVLTVEGKWDRRLGAALACYWLFLLSWIPITIHCMFQKTTV
                     WEEIRHTRNLTAVPGCGKAPAL"
     gene            complement(12598..12756)
                     /locus_tag="ACZPAQ_00180"
     CDS             complement(12598..12756)
                     /locus_tag="ACZPAQ_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR0902419.1"
                     /translation="MKKFVCDLCGYIYDEAAGAPDEGIAPGTLWADVPDDFVCPLCGA
                     DKSQFSEA"
     gene            complement(12815..14170)
                     /locus_tag="ACZPAQ_00185"
     CDS             complement(12815..14170)
                     /locus_tag="ACZPAQ_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-binding di-iron domain-containing
                     protein"
                     /protein_id="HIR0902420.1"
                     /translation="MYCIRKVTDDLLWIGGNDRQSPLFESAHPVPRGMSYNSYLLLDE
                     KTVLFDTVDRAVQTQFFENLEHALGGRRLDYVFVHHMEPDHAATLGDLMIRHPEATIV
                     CNGKSLNMIRQFHCTDPKGALLVNEGDAISTGRHSFTFYNAPMVHWPEVMVSYDAADG
                     ILFSADAFGTFGALNGILFADEVDFDRDWLDEARRYYTNIVGKYGPQVLALLKKAAGL
                     DIRMICPLHGPVWRKDLGYILDKYAKWAAYEPEVQGVLIAFASVYGGTETAANILACR
                     LAELGIPVDLYDVSVTHYSYVLSEAFKYSHLVFASTTYNNGIFVSMDNFLRDLAHHAL
                     RGRKVALIQNGSWAPASGKLMSEILCGMKDMELLEAPVTLKSTLAPGQDQELEALART
                     LAASVRGEEPALEAEPETADKPRGFVCKICGFVYESDTLPEDFTCPICRRPASDFEPL
                     A"
     gene            complement(14383..15330)
                     /locus_tag="ACZPAQ_00190"
     CDS             complement(14383..15330)
                     /locus_tag="ACZPAQ_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014542.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR0902421.1"
                     /translation="MSDSRIPLYLITGFLDSGKTTLLTDTLAMDYFNDGQRTVLLRCE
                     DGEEEYDQAELSKRNCRVVPVEDQSQICTAFLEEVDRRYQPERVLLEYNGMWPIQLLR
                     DLKLPRTWGLYQAIDVIDGTTFEMYRTNMQSMIVDMVTEADMVLFNRCTPEMPLSGWK
                     RSIRAVNRMCEIVFEDEMGEELEVEDILPYSLEEPHIDLEDDDYGIWYLDIQDHPERY
                     VDKTITFKAQAMTSMKLPRGAFIPGRNAMTCCEEDIRFFGFLCKYDRARTLKKGQWVK
                     VHAVIRWEQADVYEGEGVVLYADRVEQTDAPEDPLVYFR"
     gene            complement(15344..16516)
                     /locus_tag="ACZPAQ_00195"
     CDS             complement(15344..16516)
                     /locus_tag="ACZPAQ_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR0902422.1"
                     /translation="MTQIDIISGFLGAGKTTLIKKLLDEAFHGQKIVLIENEFGEIGI
                     DGGFLKDAGVEITEMNSGCICCSLVGDFGAALKQVLADYAPDRIVIEPSGVGKLSDVI
                     AAVERVQKEAPALQLHSFVTVVDATKAKIYMKNFGEFFNDQVEHASAILLSRTQNMDQ
                     AKLNAAVSLLRNKNPKAAILTTPWEQLTGNQIVSAMEGGRSLAEELLEEIGHSHGGDE
                     AHHHHHHEHHAGEGCSCGHDHHDHEHHDEHDHHGHDHCHDEAACDVCLGHEHGHEHHH
                     DHDEAACDACCGHHHHHGHDADEVFTSWGRETPRKYSREDLQDALSALAMSEDYGYIL
                     RAKGMVPCDDGQTWLHFDLVPEEFQIREGAADYTGRLCVIGSGLDEAGLEKLFQLV"
BASE COUNT         3058 a         4831 c         5624 g         3158 t
ORIGIN      
        1 cggcatcatg ccgtgagcct ctggctacaa aaatcgctgc aaatgtagcc aatttgtagc
       61 cactgaaaaa gaaaaatcct gaaaagcgct gtattaccaa cgcttttcag gattcagaaa
      121 acgctaaatg tttattccca ctcgatcgtt ccagtgggct tgggagtcag gtcaaagagg
      181 acacggttga cgcccttgac ctcggcggtg atacgggcgg tgatatgctg gagcaggtcg
      241 aagggcacgt tctccacggt ggcggtcatg gcgtccacgg tattgaccgc acggatgatc
      301 acacaccact cgtccgcgcg cttattgtcg cggacgccga cagatttgat gtcagggatc
      361 gcagtaaaat actgccacac cttgccctcc aggccgttct tggcaaactc ctcacggagg
      421 atggcgtcgg actcccggac ggcctccagg cggtcacggg tgatggcgcc caggcagcgc
      481 acgcccaggc cggggccggg gaagggctgg cggtacacca tggaatcggg gaggcccaga
      541 gccttaccgc aggcgcgcac ctcgtccttg aacagcatct tcaggggttc caccagctcg
      601 aagtccaggt cctccggcag gccgcccacg ttgtggtggc tcttgaccgc cttcacagtc
      661 ttggtgccgg actcgatgat gtcgggataa atggtgccct ggcccaggaa cttgatcccc
      721 tccagcttgc gggcctcctc ctcgaacaca cggatgaact ccgcgccgat gatcttgcgc
      781 ttccgctccg gatcggacac gccggccagc ttgtccagga agcggtccac cgcgtccaca
      841 tagatcaggt tggcgtccat ctcgtcccgg aacaccttga tgacctgctc aggctcaccc
      901 ttgcgcagca ggccgtggtt cacatggaca caggtcagct gcttgccaat ggctttgatg
      961 agcagagcgg ccacgacaga ggagtccacg ccgccggaca gagccagaag gaccttcttg
     1021 tcacccacct gctggcggat cagctccacc tggtcggcga taaagttctc catattccag
     1081 ttggcctgtg cgccgcagac ctggaacaca aagttgctca gtgcctcttt gcgggccgcg
     1141 gcatcctcag gccagggctg atcgcctttg tgatcggcca gcagcacggg cacaggagcg
     1201 ttgtagatct ccttggcggc gtcgatctcc acaccgtcta ccacacggtt gggacccccg
     1261 ttcaggatga tgcccttcac attcggcaga gcggcaagct cttcggcggt gatgtcgtgg
     1321 ggatggatct cgctgtaaac gcccagtgca cggatctcac gggccagacg gggattttcc
     1381 tcactgccca gatcaagtat cagaatcatg tcctgcttca ttgcagcgcc tcctattttt
     1441 tgtatgactc atagtaccac aattcgggtg gtttcgcaag atattgtgcc ttcaagcatg
     1501 gataaaatgc gtagatctta gagtagggtt cctagtatag tttgacaaaa tttgagcagg
     1561 ggagggggag tgatcccagc agcggatggc tctctccgcc agattttaca aagcttttac
     1621 ggtaagctgc ttgctgggag cgaagaaagg ggctatactc acagacagaa acggaaggag
     1681 gtgagcgcga tgggatttgc attgaatgca gccgaagatc tggaggacct gatctacgag
     1741 tgcaaggagg tctctgccag cgagcggtaa ggagatatag atccgaattt caggaccaca
     1801 ccataaggtg tggttttttg tgtttatggg atgacagcag gggggtaagc cgctccaaca
     1861 gcagaaaggt tccggaggag aggggagggg cggataaaag ttctccccgg gcccgccggg
     1921 gcccggggag aaaaggatca gatcttggtg atgtcgatgg gggtcaggtc ctcgctgcgg
     1981 ctctgctccc ggacggtgag aacggcccgg gcggtatcga tggcggtgac gcaggccacg
     2041 gagtgctcca cggcggagcg gcggatcttg aagccgtcgc tgtcctgctt gcgccccttg
     2101 gtgggggtgt tgatgatcag gtcaacggtg ccgctctgga tcatgtccag aatgttgggg
     2161 tgggcctggg agacacggtt gacccgcttg cactggatgc cagcctccgt gagggcgtcg
     2221 caggtgccgg aggtggcata gatctccacg cccatctcct cgaagccccg ggcgatgcct
     2281 accatctcgg ccttgtcctc gtccttcacg gtgacaatga tccggctgcc gcgcttgggc
     2341 accttcagac cggcgccctt gaaggccttc agcagggcct ggggataggt ctcggccaga
     2401 cccagcacct cgccggtgga cttcatctcg gggcccaggc cggtgtccac atcggtgagc
     2461 ttctcaaagg agaacacggg ggccttgacg gcgaaatagc tggcctcgcg gtacaggccg
     2521 gtgccatagc ccaggtcctt cagcttctcg cccagcatga ccttggtggc caggtcgatg
     2581 atgggcacgc cggtcacctt ggagatatag gggatggtgc gggaggagcg ggggttgacc
     2641 tcgatgacat agatgtcgtc gttgtagagg atatactggg cgttgatcag gccgatgacg
     2701 ttcaggtgct tggccatgtt cttggtgtgc ttcaggatca gctcccggtg cttctcctcc
     2761 aggttgatgg ggggatagac cgcgatggag tctccggagt ggacgccggc ccgctccacg
     2821 tgctccatga tgccggggat gaggatgtct tccccgtcaa acacgccgtc cacctccagc
     2881 tcacggccca tgaggtactt gtcgatgagg atggggtgct cctgaacggt caggttgatg
     2941 atccgcatga actcctcaat gttccggtcg gagtaggcga tctccatgcc ctggccgccc
     3001 agaacgtagg agggacggac cagaacgggg tagcccagct cgttggccgc ctgaagggcc
     3061 tcctgggtgg tatagacagt gcggcccttg gcgcggggga tggcgcactt ctccaaaatc
     3121 tcgtcaaagc gctcgcggtc ctcggcggcg tccacgccgt cggcggaggt gcccaggatc
     3181 tgcacgccca tctcggtcag cgccttggcc agcttgatgg cggtctgtcc accgaactgg
     3241 accacagcgc cccagggctt ctccagctcc acgatgttct gcacgtcctc agcggtcagc
     3301 ggctcgaagt acagcttgtc cgccacgtcg aagtcggtgg agacggtctc ggggttgttg
     3361 ttgatgatga tggtctcata gcccatgcgg cggaaggccc acacggagtg gacggagcag
     3421 tagtcaaact caatgccctg gccgatgcgg atggggccgg agcccagcac cagcaccttc
     3481 ttcttgccgg agccgttgtc caccgcctcg ttctctccgt cgtaggtgga gtagtagtag
     3541 ggggtctcgg cgtcgaactc ggcggcgcag gtgtccacca tcttgaagga ggggatgatg
     3601 ccataccgct cccgcagggc cttcacctgg gcctgggtca tgccgcacag ggcgccgatg
     3661 tagctgtcgg cgaagcacat ctccttggcg cgcttcaggg tgtccatgtc gggttcgccc
     3721 ttgcaggact tcagctcgtt ctccatggcg atgatccgct ggaagccgtc caggaaccag
     3781 atgtccatct tggtgatgtg gttgatcttt tggggggaga agccgcggcg cagggcctcg
     3841 gccaccacga agagccgctc gtcggtgacc tggaccagca ggtcctcgat ctcgtcggtg
     3901 tacaggccct ccagcttgtc cagcttcagc gcccggacgt tcagctccag ggagcggatg
     3961 gccttcatca gcgcgccctc gaaggagttg gcgatagcca tgacctcgcc ggtggccttc
     4021 atctgggtgc ccagggtgcg gctggcggtg gtgaacttgt caaagggcag gcgggggatc
     4081 ttcagcacgc agtagtcgat ggtgggctcg aagcaggcgg tggtcttacc ggtcaccgcg
     4141 ttggggatct cgtccagggt atagcccagg gctaccttgg cggccacctt ggcgatggga
     4201 tagccggtag ccttggaggc cagggcggag gagcgggaca cgcgggggtt gacctcgatg
     4261 accgcgtact catagctgtc gggattcagg gcaaactgca cgttgcagcc gccttcgatc
     4321 tccagggcgg agatgatgtc cagggcggcg gagcggagca tctggaactc ccggttggcc
     4381 agggtctggg tgggagccac cacgatggag tcgccggtgt ggacgcccac ggggtcgatg
     4441 ttctccatgg agcagatggt gatgacgttg ccggcggagt cccgcatgac ctcgaactcg
     4501 atctccttcc agccggcgat gcaccgctcg atcaggacct cgtggacacg ggacaggttc
     4561 aggccgcggt cggagatctc acgcagggag acctcgtcgt aggcgatgcc gccgccggtg
     4621 ccgcccaggg tgtaggcggg gcggacgatc acggggtagc cgatctcgtt ggtgaaggag
     4681 acggcgtcct ccacggtgtt gaccaccttg gaggtgacgc agggctgacc gatggactcc
     4741 atgcagtcct tgaagccctg gcggtcctcc gccttgtgga tggaggcggg gctggtgccc
     4801 agcaggcgca cgccgtactt ctccagggtg ccgttctcat acagcgccat ggccaggttc
     4861 aggccggtct ggccgcccat gttgggcagc agggcgtcag ggcgctcctt ttcgatgacc
     4921 tgggtgatgg agggcaggtt catgggctcg atatagacgt ggtcggcgat gtccttgtcc
     4981 gtcataatgg tggcggggtt ggagttgatc aggacgacct ccaggccctc ctccttcagc
     5041 gcgcggcagg cctgggtgcc ggcatagtca aattcggcgg cctggccgat gacgatgggg
     5101 ccggagccca gcaccaggac cttcttaata tcggtatact tcggcattac ttggcacctc
     5161 ccatggaatc aataaagcgg ttgaagaggt attccgtatc cttggggccg gcgttggcct
     5221 cggggtggaa ctgggtggta aagcagttgg ggcgcttgta cctcagtccc tcgcaggtgc
     5281 cgtcgttgac gttgatgtgg gatacctcgg ccacggcggg atccaccgtg tcggagagga
     5341 ccatatagcc gtggttctgg ctggtgatga acacgcggtt cttctccagg tcccggacgg
     5401 ggtggttgcc gccccggtgt ccgtaggcca ggcggccggt cttggcgccg gtggccaggg
     5461 ccagcagctg gtggcccatg cacacgccga agatggggat ggtgctgtca tagagcttgc
     5521 gcagctcggc gatgatctca gtgttttcct ccgggtcgcc ggggccgttg gagagcatca
     5581 cgccgtcaaa gccgccggag agcacctcgc cgacgggggt ataggcgggc agggccacca
     5641 ccttacagcc gcggcggcgc aggcactcga tgatgttctg cttcacgccg tagtccatca
     5701 cggcgaccag atgcttctcc tcgccatagg cctcgaacac ctcaggctgg gtccgggtca
     5761 ccttgcggac ggtgtccttc acctcgtagg agcgcagccg ctccatgacc tcggcgacat
     5821 taaaatgctc cgcacaggtg agcataccgt tcatgctccc ctgactgcgg agtattttcg
     5881 tcagtgcccg ggtgtccacg ccctggatgc cggtaatatc gtgttccttc agataggtgt
     5941 tcagatcgcc ctcacagcgg aaattgctgc cccgggggga cagatgacgg accacgaagg
     6001 cctccaccca gggacggctg gactcgttat cctcgtggtt gacgccgtaa ttgccgatga
     6061 ggggatagga catcaccacg ccctgtccgg cataggaggg gtcggtcagg atctcctgat
     6121 agccggtcat agaggtgttg aacaccatct cgcagatccg gtcggaggtg ccgccgatgc
     6181 tggtcccgga aaaaacgctt ccgttggcca ggatcaaagt cgctttcatc aattcactca
     6241 cacgcccttc tgttccagtt cacttagcca tatccatgtt caaagtgttt caagatattg
     6301 tatcgaaaat tcggggttat ttcaagaggg ggaactccct ttttccaaaa agatttccct
     6361 gcacttttgg gcatattgca ggaaagtccc aggagagact ttcccggcgg gctgggatag
     6421 tacgaaggaa aaccgggaaa actgtgtggg agcggccgcc gtccacggcg tcagggccgc
     6481 agaacatcga cggaaggagc gaaaaactgt gttcaccgga ctgatccgca ccatcatcct
     6541 atacatcctc atcatcgccg ggatccggct gatgggcaag cgccaggtgg gggagctgga
     6601 gccctcggaa ctggtgctct ccctgatcat cgcggacctg gcggcggtgc ccatgcagga
     6661 tttcggcatc ccgctgctca ccggggtggt ccccatcctg gccctgctgg ccatgaccat
     6721 gatcctgtcg gtgctgacca tgaaaagcat ccggttccgg gcgctgctgt gcgggcggcc
     6781 cagcgtggtc atccgccggg gggagatcga ccagggggag atgcggcgca accggctgac
     6841 ggtggacgag ctgctggagg agctgcgcag ccagggctat acagacctgc aaaaggtgga
     6901 gtacgccatt ctggagacca acgggcagct ctctgtcctg ccgcgggcgg cggagaagcc
     6961 gcccaccctg ggacagctgg gactggaggc ccaggagtcc gggctgccgc tggtgctcat
     7021 cagtgacggg cggctgctgg agcggaacct ccgggtccgg ggctatgacg ggcactggct
     7081 ggacaagcag ctggagcagc ggggcttgaa gcgctgggag gaggtctttt tgatgacggt
     7141 ggacgagcag ggacagatct atctggctcc ccgggagcgg gaggggaggc ggggcgcgtg
     7201 aagcggctgt ggagcgcggc ggcgctgctg ctggtcctgc tggggggagc gctgtggaac
     7261 gcgtggtacg cggaccggct caccgggcgg atggccgggc agctggaaca ggcccaggag
     7321 ctggccgacc ggggagactg ggagggggcg gtccggctca gccgggagag ctttcacctc
     7381 tggcaggccc atgacgccta tcttcatgtg atgatgcgcc actcggacac cgaccagatc
     7441 ctccggggct tccgggccct ggatcagtac ctggccctgg aggagtccga ccagtacacc
     7501 gccgccaacg cggagctgat ctgtcagctg gagctgctgt cggagatgga gcagccctcc
     7561 ctggtaaata ttttttaaga agagacagcc cccggatctg acgcagtcag gtccgggggc
     7621 tgtcttgaaa cggagcggtt caggccggga gcagcccggc ggagatcagg aaatcccggg
     7681 ccacatcgtc caccgcctcg ccttccacat ccacacggta ggtcagctcg ctcattttct
     7741 catcggtgaa ctggccgttg agcagctgga gggtctcctt gaggccgggg gcctcctcag
     7801 cgaagcgctc aaacaggtcg ctgcgtacca ggatggtgcc gtagtagtcg gggaagaagt
     7861 ggagatcgtc cttcagaatg gtcaggttgg cccgcttatt cagaccgtcg gtggcataga
     7921 ccaccatcac gtcgtacgcg ccctgctcca ccatagtgta cttcatcata atgtccacat
     7981 ggagcacgtc ctggaagttc aggccgtaga acttggaaaa cgggccgaac ttcatgctgc
     8041 cggcgtcggt gaagaagtcc tgctccgcgc cgaagcgcag ctggccggcg atggggatca
     8101 ggtcgctgat ggtctcgggg tgataggtgt ccatcacttg ctgagtgacg ccgatggagt
     8161 aggtgttgtt cacaccgatc tttcccatca gctccagccc gtactccccg cgcatccgct
     8221 ggtccacaaa ttcatagagg gtctgaccgt ctgggacatc ggaggtgtcc aggcccatga
     8281 tggtggtcag cagggtcccg tcccaggtat acatcaggtc gcaggagtgc cccgccccgg
     8341 acagctccgc aaagttgttc ttggcggtca tctggtcctt gatggtgaca gggatgccgt
     8401 ggcgctcctc caccagctgc tggatcatgc tgttgaccag ctgcacctcg gaaaattcgc
     8461 cctggtagag gaccagaccg tcggtgcgct gggggaagaa ggccaccacg cacagggcag
     8521 ccgcggcaac gcaggcagcg gcggaggcgg cccgggcaat gaggcggctg gttcgggagc
     8581 gctggccggt gcggctgttc agatagcgct ccgccagtcc catcagcaga tccaggacca
     8641 gggccatggc catcagcacg ccggtgccca gcaggatggc ctccatatca tcctgacgga
     8701 tgccggtggt gatgaagctg cccagtccac cgccccccac gaaggaggca aagaccgcgg
     8761 tgccgatggc gttgaccgtg gcgatgcgga tgccggtgaa gatgatgggg gcggccaggg
     8821 ggagctccac atggagcagg cggtacatcc gggtcatgcc catgccccgg ccggcctcga
     8881 tgaggtactc cggcacctgg ctcaggccca ggtgggtgtt ccgtacgatg ggcagcaggg
     8941 agtagagggc cagaccgacg atgacggtgg gcttgcccgc ccccatgaag accatgatga
     9001 tgcccagaag ggccagggcg ggggtggtct ggatcaggtc caccacccgg agcaccaccc
     9061 gccgggcgga ggggtagaag tagcacagga tgcccagtgg gacgccggcc agaatggcaa
     9121 agaccagggc ggccaccaca agggccagat gagtcaggat catggagagg gtcatgtggc
     9181 agaacctccc ttccgcagcc ccttgggcac cgtccggcgc tggagcagat ccaccagggt
     9241 gcccagggcg aaggccagca gggccgcggg gatggccccc agcaggatca gggcgttgtc
     9301 attggagctc aggccggcgt agatgaagtc gcccaggccg cccaggccga tcatggaggc
     9361 cagcaccgcc cagctgacgg tgtagacggt ggacatccga agcccgccga tgatagtggg
     9421 cagggccagg ggcagctcca cccggagcag gctctgccac gggctcatgc cgcagccccg
     9481 ggcggcctcc agatactgcg ggtccacgga caccaggccg gcgtaggtgt ttttcagcac
     9541 cgggaaggtg gcgtagatcc ccagggcggc cagcacggtg gccgcgtggg tgccccaggc
     9601 caggaacagc aggccgatga acacgatgcc ggggatggtg ttgaagatgg acaggatggg
     9661 cagcaccacg ccggacagcc tgggccgccg ggacagcagg acgcccagaa ccacgcccag
     9721 cgcgaagccg atggccaccg aggccagggt gaagaacagg tgctccgcca gcttgacggg
     9781 gaggtcatgg atgctcatgc gtcctcaccc ccaaagctgt tccgccatgg cggaggccat
     9841 gctggtcttg gtgatgatgc ccgccacctg cccctggccg tccagcacca ccagataggg
     9901 cgcgccgctg gagatcagct ggtcgaagca gtcctttgcg ttgtcctccg catggacggt
     9961 gggtgtgttg cagcggatca ggggctcgat ggtctggacc acatggccgg tgagacggat
    10021 gtccgcgatg gagacggtac cctgataccg ggcttggtcg tccaccacga tcagggtgtc
    10081 cacgttgtgg cgctgcatct tgctgatgca ctcgttgatc cccttccggg tggagacggt
    10141 gtagacgccg gtgcgcatga agtcggcggc ggtgagttcc ttgtgggcct gaaggtcgat
    10201 gaatttgccc aaaaagtcct ggatctggcc gctggcggga tgggagagca tctcctccgg
    10261 gggagccatc tgaacgatcc tgccatgatc catgaagatg ataacgtctg ccaggtccag
    10321 ggcctccgcc atgtcgtggg tgacaaagac gaaggtcttg ttcagcttct gctggagggt
    10381 gcgcacctcc tgctggaggg tacggcgggt catggggtcc agggcggaga agggctcatc
    10441 catcagaacg atgggagggg aggcggccag ggcccgcagg atgccgatgc gctgctgctg
    10501 tccaccggac atctccgagg ggtacttgtg ggcgtactgt tcatagggca ggttgaccat
    10561 gtccagcagc tcacgaacga tgcggtcgca ctcctcccgg ctgtacttga gcagggtggg
    10621 gaccacgctg atgttctggg ccaccgtcat attggggaac aggccgatct gctggatcac
    10681 atagccgatg tggcggcgca actccacctt gtccttggta gtgatgtcct gtccatccag
    10741 atagattctg cccgcggttg gctcaatgag gcggttgatc atcttcagcg tggtggtctt
    10801 tccacagccg gacgggccga tgagcaccgc catctgtccc tcctggatct caaaggagag
    10861 gtcatccagg atctgctggc tgccgtagga gagggaaacg tgttcaaaac gaagcaaagg
    10921 ggcacctcct tactgcggcg tgcccctgag aaacaggaaa atgttgagaa ttggaagggg
    10981 gcaggccggg gaatcttgaa aaatcaggta aaatatataa attattatag taaaataaag
    11041 aaaaatagtc aaataaacaa attgtaaatt ttgcggccgg tcaagcctga ggcgggattt
    11101 cacacacttt tagtcaaaac ttaagaaagt ccccggggat ttcttaagat tcctctggta
    11161 gaatccggtc aaaaccggac agaggaggag tctgtatgag ctttctgacg gcggctgcgg
    11221 cgctgctggg cgccgggatc accctttggg ggagctatta tctgctggta ggcctgatgt
    11281 cctggcggcg gcccatggag tacggcagac atccggcctc cacgcgcttt gccgtgctgg
    11341 tcccggcgcg gaatgaggag ctggtggtgg gcagcctgat caataccctt ctgatgcagg
    11401 actatccccc ggagctctac gatatatggg tggtccccaa tcaatgcacg gatcataccg
    11461 cgctggcagc ccggcgcttt ggggcccatg tgctggagtg taccgttccg gtgaagagca
    11521 agggcgaggt gctgcgcttt gcgtgggagc ggctggcgca ccggggctat gacgcggtct
    11581 gcgtctttga cgcggacaac ctggcggacc cccagttcct gcgggagatg aacaacgcct
    11641 accgggcggg ggcacgggta gcccagggct ttcgggacag caagaacccc tatgacagcg
    11701 tggtgtccgg ctgctactcc atctactatt ggatgatgga ccgcctccac aacgggggcc
    11761 gggccgggct gggcctgccc gccatgatca acggcaccgg ctttatggtg gccgcttccg
    11821 tcctggagcg aatgggcggc tggcggacgg agaccatcag cgaggatctg gagctgtcag
    11881 tccagtgcgc cctggaggag gtccaggtct tttgggtccc caaggcggtc acctatgacg
    11941 agcagcccct gaccttccgg gagtcggtga agcagcgcag gcgctggacc agcgggaccc
    12001 tccaggtagc cgggagctat ctgcccgcag tggggcgggc gctggcggca cggccctccg
    12061 gcgcgacctg ggatgtaggc gccaccctgc tgatcccggc ttaccagatg gcggcgctgg
    12121 tcaatctggc ggtctctgcc ctggcggcgg gccaaatggg gccggacggt ttttcgcccc
    12181 tgctgtgcgc ggcgggcgcg gcgggcagcc tggccattgc cgccctgggg gccacgctcc
    12241 tggcggccct tgtgctgacg gtggagggga agtgggaccg gcggctggga gcggccctgg
    12301 cctgctactg gctgtttctg ctcagctgga tccccattac catccactgc atgttccaaa
    12361 agaccaccgt ttgggaggag atccggcata cccggaacct gacagccgtt ccgggctgcg
    12421 gaaaggcccc ggcgctgtaa atcccggagg acacgggaac agccctgcgg cgtgcagcag
    12481 atggactttg tccggcggtg cggccgtggg aaagaagaaa caatgcccca ggcgctttgg
    12541 agcgcctggg gcattgcagt ctgttggaaa agactgagct gcagatgggg gagaggctca
    12601 ggcctcagag aactggctct tgtccgcgcc gcacagggga cagacaaagt cgtcgggcac
    12661 atcggcccac agggtgccgg gggcgatccc ctcatcgggg gcgccggcgg cctcgtcata
    12721 gatatagccg cagagatcac agacaaactt tttcataaag acaacctgct ttccattcag
    12781 atttgtgccg gagcgctccg gctgggggct ggggtcaggc cagcggctcg aagtcgctgg
    12841 cgggacggcg gcagatggga caggtgaagt cctccgggag ggtgtccgac tcatagacaa
    12901 agccgcagat cttgcagacg aagccccggg gcttgtccgc tgtctcgggt tccgcctcca
    12961 gggcgggctc ctcgccgcgg acggaggcgg ccagggtccg ggccagggcc tccagctcct
    13021 ggtcctgtcc gggggccagg gtggacttca gggtgaccgg agcctccagc agttccatgt
    13081 ccttcatgcc gcacaggatc tccgacatga gcttgccgct ggcaggggcc caggagccgt
    13141 tctggatgag agccaccttg cggccgcgga gggcgtggtg ggccagatcc cggaggaaat
    13201 tgtccatgga gacgaagatg ccgttgttgt aggtggtgga ggcaaagacg aggtggctgt
    13261 acttgaaggc ctcagacagc acataggagt agtgggtgac cgatacgtcg tacaggtcta
    13321 cagggatgcc cagctccgcc agacggcagg ccaggatatt ggcggcggtc tccgtcccgc
    13381 catagaccga ggcgaaggcg atgagcacgc cctgaacctc gggctcatag gcagcccact
    13441 tggcgtactt gtccaaaata tagcccagat ccttccgcca caccgggccg tggaggggac
    13501 agatcatccg gatgtccagg ccggcggcct tcttcagaag ggccaggacc tgggggccgt
    13561 acttgcccac gatgttggtg taatagcgcc gggcctcgtc cagccagtcc cggtcaaagt
    13621 ccacctcgtc ggcgaacagg atgccgttga gggcgccgaa ggtgccgaag gcgtcggcag
    13681 agaacagaat cccgtctgcg gcgtcatagc tcaccatcac ctccggccag tggaccatgg
    13741 gggcgttgta gaaggtgaag ctgtggcggc cggtggagat ggcgtccccc tcgttgacca
    13801 gcagggcccc cttgggatcg gtacagtgga actggcggat catattcagg gacttgccgt
    13861 tgcagacgat ggtggcctcc gggtggcgga tcatcagatc ccccagggtg gcggcgtggt
    13921 cgggctccat gtggtggacg aacacatagt ccaggcggcg gccgcccagg gcgtgctcca
    13981 gattttcaaa gaactgggtc tggaccgcgc ggtctaccgt gtcgaacagg acggtctttt
    14041 catccagcag gaggtaggag ttgtaggaca tcccgcgggg gacggggtga gcgctctcga
    14101 agagagggct ctggcggtcg ttgccgccga tccagagaag gtcatcggtg acctttcgga
    14161 tgcagtacat atcaaaacct cttttctata agtaggcttg ggcagcgtct tacaggaaca
    14221 tcatagcata gtctgcgccg gaatggtagt aaaaaaacac atttcgggaa aaatcacaag
    14281 acagatcaaa ataataggaa aaataaaaga caggatgcag aaaacgcggg ccgctgcttg
    14341 atgcagcggc ccgcgccggc ggggttcatc cgtgctttgc gctcagcgga aatacaccag
    14401 gggatcctcc ggggcgtcgg tctgctccac ccggtcggca tagaggacca cgccctcccc
    14461 ctcatagacg tcggcctgct cccagcggat gacggcgtgg accttgaccc actggccctt
    14521 tttcagggtg cgggcccggt cgtacttgca caggaagccg aagaagcgga tgtcctcctc
    14581 gcagcaggtc atggcgttgc gcccggggat gaaggcgccc cggggcagct tcatactggt
    14641 catggcctgg gctttgaagg tgatggtctt gtccacatac cgctccgggt ggtcctggat
    14701 gtccaggtac cagatgccgt agtcgtcgtc ctccagatcg atgtggggct cttccaggga
    14761 gtaggggagg atgtcctcca cctccagctc ctcgcccatc tcgtcctcaa agacgatctc
    14821 acacatccgg ttgaccgccc ggatggaccg cttccagccg gagaggggca tctccggggt
    14881 gcagcggttg aacagcacca tgtccgcctc ggtcaccatg tccacgatca tggactgcat
    14941 attggtgcgg tacatctcga aggtggtgcc gtcgatgacg tcgatggcct gatagaggcc
    15001 ccaggtccgg ggcagcttca ggtcccgcaa cagctggatg ggccacatcc cgttgtactc
    15061 cagcaggacg cgctcgggct gatagcggcg gtccacctcc tccaggaagg cggtgcagat
    15121 ctgggactgg tcctccaccg ggaccacccg gcagttgcgc ttggacagct ccgcctggtc
    15181 gtactcctcc tcgccgtcct cgcaccggag gagcactgtg cgctgtccgt cattaaagta
    15241 gtccatagcc agggtgtcgg tgagcagggt ggtcttgccg ctgtccagaa agccggtgat
    15301 gaggtacagg gggatccggg aatcactcat gtcggcgacc ccctcacacc agctggaaca
    15361 gcttttccag cccggcctcg tccagcccgg agccgatgac gcacaggcgg ccggtgtagt
    15421 cggcggcgcc ctcccggatc tggaactcct cagggaccag gtcgaagtgg agccaggtct
    15481 gcccgtcgtc gcaggggacc atgcccttgg cccggaggat atagccgtag tcctcgctca
    15541 tggccagggc ggagagggcg tcctgaaggt cctcccggct gtacttgcgg ggggtctcgc
    15601 ggccccagga ggtgaacacc tcgtcggcgt cgtgtccgtg gtgatggtgg tggccgcagc
    15661 aggcgtcgca ggcagcctcg tcgtggtcgt ggtggtgttc gtggccgtgc tcgtgaccca
    15721 ggcagacgtc gcaggccgcc tcgtcgtggc agtggtcatg cccgtggtgg tcatgctcat
    15781 catggtgctc atggtcgtgg tggtcatggc cgcaggagca gccctcgccg gcatggtgct
    15841 cgtggtggtg atggtggtgc gcctcatccc cgccgtggct gtggccgatc tcctccagca
    15901 gctcctccgc cagggagcgg ccgccctcca tggcggacac gatctggttc ccggtgagct
    15961 gctcccaggg ggtggtgagg atggcggcct tggggttctt gttccgcagc aggctgacag
    16021 ccgcgttcag cttggcctgg tccatgttct gggtgcggga gaggaggatg gcggaggcgt
    16081 gttccacctg gtcgttgaag aactcgccga agttcttcat atagatcttg gccttggtgg
    16141 cgtcgaccac ggtgacaaag ctgtggagct ggagagcggg ggcctccttc tgcacccgct
    16201 ccacggcggc gatgacgtcg gacagcttgc ccacgccgga gggctcgatg acgatgcggt
    16261 cgggggcgta gtcggccagt acctgcttca gggcggcgcc gaagtcgccc accagggagc
    16321 agcagatgca gccggagttc atctcggtga tctccacgcc ggcgtccttg agaaagccgc
    16381 cgtcgatgcc gatctcgccg aactcatttt caatgaggac gatcttctga ccgtggaagg
    16441 cctcgtccag cagctttttg atgagagtgg tcttgccggc ccctaaaaag ccggagataa
    16501 tgtcgatctg tgtcatagta ttgccatatc ctttctgata aatcaagagt aatcaaggaa
    16561 aaacgccctc cctctgggag agggcagttc atacagggtc agtcccagag caggccggcg
    16621 gactccagca gggcgtggag caggccggcg gcggtctccc gggtgccggg c
//
LOCUS       DBMDSJ010000003        15750 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_1386_length_15750_cov_2.356828, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000003 DBMDSJ010000000
VERSION     DBMDSJ010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15750)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1386_length_15750_cov_2.356828"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(77..1324)
                     /locus_tag="ACZPAQ_00200"
     CDS             complement(77..1324)
                     /locus_tag="ACZPAQ_00200"
                     /EC_number="4.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000096 - sulfur amino acid metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR0902423.1"
                     /translation="MSLQISERVRALGRQAQQDLREQFDRIDAIAEENSIRVLEAFQD
                     HRVAEGYFAGTTGYGYDDLGRDKLDEIYAQLFGTEAALVRIQFVNGTHAIACALYGAL
                     KTGDVLVSAVGAPYDTLLGVIGETGKGHGSLKDYGVEYRQVELKDNKPDLEGMARAAA
                     DPRVKAVLIQRSKGYSTRASLSVAEIGEMCRVIREANPNAAILVDNCYGEFVETMEPT
                     HVGADLVVGSLIKNPGGGLAPTGGYIAGRRDLVEGAAQRLTVPGIGGECGSTLGNNRL
                     LYQGLFLAPHTVAQAVKTAVFAARVMELLGYETEPHSSAVRHDIIQMIHTREPEALKK
                     FCKGIQFGAPVDSYVTPEPWDMPGYDCPVIMAAGAFIQGASIELSADAPMRPPYTVYL
                     QGGLTFESGKLGVLLAVEELLRA"
     gene            complement(1378..2379)
                     /locus_tag="ACZPAQ_00205"
     CDS             complement(1378..2379)
                     /locus_tag="ACZPAQ_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR0902424.1"
                     /translation="MELFELIQTLNTAHGPSGDEGAVREVIAALARPHADEVTADTLG
                     NLIVRKRGSGPKVMLCAHMDSTGLIVTHIEKEGFLRVGTLGGIAARELLYTPVRFKNG
                     VRGVIVPEEKAEFGKLKLDACYVDIGAKDEEQARRMVQVGDTAVYDTAIFLSGDKVVS
                     PYLDNRISCAVLLRVLEELDACPNDLYLVFSAQEEVGLRGARTAAWSIDPDYALAVDV
                     TDVDDTPGSEKLGTVRLGQGAAVKVMDRSVICHPQLVETLERLAGEREVPVQRDIMRG
                     GGTDAGAIHTTRMGVLTGGVSVPCRYIHTPTEMADLGDAAACVKLLAAFAQEELTRL"
     gene            complement(2398..3429)
                     /locus_tag="ACZPAQ_00210"
     CDS             complement(2398..3429)
                     /locus_tag="ACZPAQ_00210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR0902425.1"
                     /translation="MIQCLETLCALNGVSGDEGQVRSYIRGWAEEHADSVRTDALGNL
                     IVFKRGRKAAENRLMLCAHMDEVGVIVTRATDEGFLKFDFVGGVDRRVALGKPVELGP
                     GRVPGVIGMKAIHLLSREDMKKVPKTDALYLDIGASSREEALSMVPLGTCGAFVGPPE
                     RFGEGLFKAKAIDDRVGCAVMLELLREKLPMDVTFAFTAQEEVGTRGAFGAAFSVTPE
                     VALVLETTTAADLPMVEAHRKVCAPGKGPVISYMDGGTIYDRGLFEDLRRLAEEHRIP
                     WQTKEYIAGGNDARTIQRTKSGVRVAALSAAVRYLHAPASVGSIFDFEAMLKLTRLFL
                     ADQAAQLTL"
     gene            complement(3426..4463)
                     /locus_tag="ACZPAQ_00215"
     CDS             complement(3426..4463)
                     /locus_tag="ACZPAQ_00215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR0902426.1"
                     /translation="MDYEKTLAGLCALPGPSGFEGQAARAALELLRPLVDEAFLDRMG
                     SVVGVRRCGREGAKKVLLDAHLDEIGFLITGHEEGFLRFAPLGGVDPRMLPDRELTIL
                     TQPPVVGVVSCLPPHIQSREDMDQSVAIQDLYLDAGLDQETAQARIPVGTPAVYRAGC
                     APLGDKLLCGKALDDRACFAILLDALERLRDRQLDVDLYVLGSTQEETHSTGAIAAAY
                     GIAPDCCVAVDVTHGDSPDASKDKTFPLGGGPVIGLGPNCTRWMARRLERKAEELGLD
                     VQLEVMAGSSGTNGWPMQISREGIATAVLSLPLRYMHTPIETVHRDDLDYTAQLLAAF
                     LEGLGEEAEAL"
     gene            complement(4486..5868)
                     /gene="murD"
                     /locus_tag="ACZPAQ_00220"
     CDS             complement(4486..5868)
                     /gene="murD"
                     /locus_tag="ACZPAQ_00220"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR0902427.1"
                     /translation="MSTIKEYLEEIQGRPVAVIGMGVSNTPLIKMLLRAGAEVTVRDR
                     VPRERAAEQVQELESLGARMILGEEYLQDLHEDVIFRTPGLSPNTPELVNAVQRGIEL
                     SSEMELFFQTCPSRLIGVTGSDGKTTTTTIIAEFLKEAGRNVYVGGNIGKPLLPDVAD
                     MVEEDFAVLELSSFQLMTMEQSPHIAVVTNLSPNHLDYHHTIEEYVRAKKNIFLHQGP
                     EDRLILNYDNAPTRALAREAVCPVTFFSRQERLEEGVYLRDGAIWLTNDQGSREVLPL
                     ELIQLPGDHNVENYMAAIAAVDGLVPDRCVRAVARRFQGVEHRIEFVRELDGVRWYND
                     SIGTSPSRTTACLESFDRKVVLIAGGYDKGIPFTQLGMEIVDRVKALILTGDTASAIR
                     SAAEQAPSFEASGLVIREEEDLAAAVRAARETAREGDVVVLSPACAAFDQFKNFMERG
                     QAFKRLVQAL"
     gene            6041..6391
                     /gene="rnpB"
                     /locus_tag="ACZPAQ_00225"
     ncRNA           6041..6391
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPAQ_00225"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6446..6865)
                     /locus_tag="ACZPAQ_00230"
     CDS             complement(6446..6865)
                     /locus_tag="ACZPAQ_00230"
                     /inference="COORDINATES: protein motif:HMM:NF039403.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6291 domain-containing protein"
                     /protein_id="HIR0902428.1"
                     /translation="MNNPKRGFVVYFDNYPMLLTMPPEQRGLLFTALMQYADGRWRGE
                     VTDPEEVLVRWPDMGAQAQMGFRFMASAVDRDTQRWLLRRQAGERRRQQTREGERGAP
                     APSSPAPRARTEPPDARYSADLEQTRRLVERIRSGGA"
     gene            7141..7860
                     /locus_tag="ACZPAQ_00235"
     CDS             7141..7860
                     /locus_tag="ACZPAQ_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015378.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR0902429.1"
                     /translation="MKKSYIAAALALTSCMVVGTAAASQSNSVTATFRPDMTLKVNGT
                     AYTVRDTTGVKVSPLIYNGTTYLPLSSLGQLLGAEVSWDNDSQTVIIHDDDADYIGEA
                     KAKELALKHAGLSSKEVSFLQLKLDWDDGRAVYEVEFYAGSREYDYEINAVTGAVVDF
                     DSDIEDYTIPSDSEDYISREKAQKLAQAKAPKATLIKLELDVDDGRAVYEGELREGST
                     EYEFEIDAVTGSFLKWEQERD"
     gene            complement(7946..9757)
                     /locus_tag="ACZPAQ_00240"
     CDS             complement(7946..9757)
                     /locus_tag="ACZPAQ_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HIR0902430.1"
                     /translation="MLFHCTKGDCRFWGEGAAMPDACPRCGGALTAGERAKMSGDDWC
                     ALGVFWMEQDGAEEKAVSCFRRAARLGSGWGACNLGLCLEQGTGIEADPRQAVWLYQQ
                     AAEMGSLPALCNLAVCYEEGIGIPPDPRQAVELYRRAAEYGSTRAQRLLARCCDRGIG
                     MDRRDPAQAVEWLRMAALQGDTVCQTLLAKHYEFGDGVEQDPELSVRWYRRAAEGGEP
                     EAKCCLGWLMEHGKWADRDPEGAVELYRQAAEAGSERGLLLLAGCLLSGVGTQAAPEQ
                     AVRCYEKGAEAGSAQAMCNLGWCYETGNGVEQSWEQAVLWYRRSAEEGDARAMCNLGW
                     CYESGNGVEQSWEEAVHWYRRGAEGGDSQAMCNLGWCYESGNGVEQSWEEAFCWYRKG
                     VEKGSTRAMFQLAWCYEKGLGVERDPAQAADWYRRGAEAGSTACMNNLAELLAKGDGV
                     PQDLEQAMEWYRRAAELDDECAWYNLGWYHEQAGELDQALECLLRADELGDDSACWGL
                     GRFYETGTAVEQDLEQAFAYYRKGAEREDLNSLCRLARCYALGIGTRKDREKASALAQ
                     EILSRDWEESGELEDYGARAELEALRALWKELEPEKP"
     gene            complement(9799..11262)
                     /locus_tag="ACZPAQ_00245"
     CDS             complement(9799..11262)
                     /locus_tag="ACZPAQ_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR0902431.1"
                     /translation="MAAKAIMIQGTMSNAGKSVLTAGLCRIFRQDGYSVAPFKSQNMA
                     LNSFVTREGLEMGRAQVMQAEAAGVEPSVRMNPILLKPSSDMGSQVIVNGEVLGDMRA
                     SDYFRHKKELVPQILASYRSLAEEHDVIVLEGAGSPAEINLKADDIVNMGMARMARAP
                     VLLAGDIDRGGVFASLYGTVALLEAGERAMVKGTVINKFRGDAALLRPGLTMLEELTQ
                     IPVVGVVPWLELDLDDEDSLSPRLTSGRREALLDVAVVRLPHISNFTDFNALGRHPAV
                     GLRYTDRPEDLGRPDLVILPGTKSTLADLDWLRRRGLDRAVLALAAAGTPVAGICGGY
                     QMLGQRVDDPEGAEGGGSQEGLGLLPVATRFSREKTRTRRRAAFPACTGLFAPLEGCP
                     LEGYEIHMGDSGPEGLFSVRENVLGTYLHGFFDTPELRDRLVSLLLARRGLTWTGETG
                     DEDGQAYREEQYDKLAAALRASLDLEAVYRILEQGLD"
     gene            complement(11264..12313)
                     /locus_tag="ACZPAQ_00250"
     CDS             complement(11264..12313)
                     /locus_tag="ACZPAQ_00250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0902432.1"
                     /translation="MARFEHGGDTYGCPPDLLDCSINLNPMGAPGPVLEGACRAVLDC
                     ARYPDPACRGLRRAIARRDGVEEDQILCGNGASDLIYRMALACKLRQALLLAPTFSEY
                     RRALEGVGCQIREHRLEERTGFLPGEDLLEAIVPGVDLVFLCDPNNPTGRLMDGGLLG
                     RVLSRCREVGAFLAVDQCFLELTGGDPRRLTEELSGGGLLLLRALTKSYALAGLRLGY
                     ALCGEEALLDRMAAAGPPWAVSVPAQAAGEIALQQFSRWPKDSLGAIRRGRERLAAGL
                     TGLGLWVCPGDGNFLLFRGPEDLGRRLLEREHILVRDCSDYSGLGPGWFRIGVRTEEE
                     NERLLTAAARQLRRD"
     gene            complement(12579..13553)
                     /gene="cbiB"
                     /locus_tag="ACZPAQ_00255"
     CDS             complement(12579..13553)
                     /gene="cbiB"
                     /locus_tag="ACZPAQ_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR0902433.1"
                     /translation="MTLTAHLITVLLPGVVLDWLLGDPHWMPHPVRWMGRAITWLEGV
                     LRRVFPGTPAGERLAGALLALLIPAAFGGGSWLLLEGLRGLSPCLAWPVEVWFTYQLL
                     AARALQKESMAVCPPLAAGDLAGAREAVSRIVGRDTQRLDEAGVVKAAVETVAENTSD
                     GVTAPLLFLALGGLPLGMVYKAVNTMDSMVGYRNERYRWFGTAAARLDDVLNWIPARL
                     SGLMMCLAAALLPGCSGRRALRVFFRDRRKHSSPNSAHTEAACAGALGVQLAGDAWYF
                     GKKVSKPTIGDGLRPVERADVARACRLMYVTELLTLAAVLGWGTLLAA"
     gene            complement(13550..14128)
                     /locus_tag="ACZPAQ_00260"
     CDS             complement(13550..14128)
                     /locus_tag="ACZPAQ_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012521.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR0902434.1"
                     /translation="MELLLLRHSITPGNLKRQYIGSTDQPLAPEGEELARKRRGEMPE
                     IGGLWVSPMLRCRQTAALLFPGVEQRQVPQLRECAFGRFEEKTWEELKDDPLYRAWMG
                     GDLTITFPGGENMQGFLERSRRGIEAVVREAEAAGVDRGAVVAHGGTWLAVMSAFGRP
                     EWELYRWQPENCRGWRVEVRREPLELRVLEAL"
     gene            complement(14223..14537)
                     /locus_tag="ACZPAQ_00265"
     CDS             complement(14223..14537)
                     /locus_tag="ACZPAQ_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR0902435.1"
                     /translation="MILVIGGRGAGKRSFVTDVLGFSPEQFAENGPVLYGLEELDPLP
                     PLEALLGRAVVVCREVGCGVVPMDRGDRDRREAVGRLCCDLAREAEAVYRLQCGLAMR
                     LK"
     gene            complement(14534..15304)
                     /locus_tag="ACZPAQ_00270"
     CDS             complement(14534..15304)
                     /locus_tag="ACZPAQ_00270"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR0902436.1"
                     /translation="MTVLRAVGMAFALYSRIPVPPLDWESGARRYTLYAFPLVGLAVG
                     GGLLLWTWLAGALGLGQLLWAAGMTAIPLLITGGIHLDGFCDVCDALASHQSRERKLE
                     IMADPHVGAFAVQGCGLLLLLTFGLWCQAERTDGGLWAALLLVPVLSRGLSAFAALTL
                     PNARGGGLLAAVTGERRRGRWLPLCGALLCAAGLAVLKPGYLWIGGAALLALWYYEKT
                     AKREFGGTTGDLAGWFLQLCELACLVGLVLAQSLEGVQ"
     gene            complement(15301..>15750)
                     /locus_tag="ACZPAQ_00275"
     CDS             complement(15301..>15750)
                     /locus_tag="ACZPAQ_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014351.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR0902437.1"
                     /translation="PLIYLATMEPWGGEAAARIGRHKALRAGKGFSCRVERPRDLAGW
                     APPAGSAVLLEDLGNLAANELFSGGAFDPEGAYRRIRRGLDRLRGGAGHLVVVSGDLF
                     RDGVDYPPETGAYLDLLARLHRDLAARADRVTELVCGLPVHWKGGEV"
BASE COUNT         2647 a         5849 c         4774 g         2480 t
ORIGIN      
        1 gtccagcccc tccatggcgc tccctccctc tccgctccgg cctgatatgg ggagggtatg
       61 cgccgtccgg cttggactat gcccgcagca gctcctccac cgccagcagc acccccagct
      121 tgcccgactc gaaggtcagg ccgccctgga ggtacaccgt gtaagggggg cgcatggggg
      181 cgtcggcgga cagctcgatg gaggccccct ggatgaaggc ccctgccgcc atgatgaccg
      241 ggcagtcata gcccggcata tcccagggct ccggggtgac ataggagtcc accggagcgc
      301 cgaactggat gcccttgcag aactttttca gagcctccgg ctcccgggta tggatcatct
      361 gaatgatgtc gtggcgcacc gcgctggagt gtggctcggt ctcatagccc agcagctcca
      421 tcacccgggc ggcaaaaacg gcagtcttga ccgcctgtgc caccgtgtgg ggggccagga
      481 acagcccctg gtacagcagg cggttgtttc ccagggtgga gccgcactcc ccaccgatgc
      541 ccggcacggt gagccgctgg gcggccccct ccaccagatc ccggcggccg gcgatatagc
      601 cgccggtggg ggccaggccg cccccggggt tcttgatgag ggagcccacc accaggtcgg
      661 cccccacatg ggtgggttcc atggtctcca caaactcccc atagcagttg tccaccagga
      721 tggcggcgtt ggggttggcc tcccggatca cccggcacat ctccccgatc tccgccacgg
      781 acagagaggc gcgggtggag taacccttgg accgctggat cagcaccgcc ttcacccggg
      841 ggtctgcggc cgcccgggcc atcccctcca ggtcgggctt gttgtccttc aactccacct
      901 ggcggtactc cacaccgtag tccttcaggg agccgtggcc cttcccagtc tctccaatga
      961 cgcccagcag ggtgtcgtac ggagcgccca ccgcggacac cagcacgtcc ccggttttca
     1021 gggccccata cagggcacag gcgatggcgt gggtgccgtt gacaaactgg atgcgcacca
     1081 gggcggcctc tgtgccgaat agctgggcat agatctcgtc cagcttgtcc cggcccaggt
     1141 cgtcatagcc gtagccggtg gtgccggcaa aatacccctc cgccacccgg tggtcctgga
     1201 aggcctccag cacccggatg gaattttcct ccgcaatggc gtcaatgcgg tcaaactgct
     1261 ctctcaggtc ctgctgcgcc tgacggccca gggcgcggac ccgctcactg atctgtaaac
     1321 tcatattcct ctacgcttcc tgtttctatt gtaatattga gggagcttcg cccggcatta
     1381 cagccgggtc agctcctcct gggcaaaggc ggccagcagc ttcacgcagg ccgccgcatc
     1441 ccccaggtca gccatctccg tgggggtgtg gatataccgg caggggacgg aaacgcctcc
     1501 tgtgagcact cccatgcggg tggtatggat ggcccccgcg tcggtgccgc cgccccgcat
     1561 gatgtcccgc tgaacgggga cctctctctc tccggccaga cgctccaggg tctccaccag
     1621 ctgagggtgg cagatcaccg agcggtccat caccttgacc gccgctccct ggcccagacg
     1681 gacggtgccc agcttctccg agccgggggt gtcgtccacg tcagtgacgt ccaccgccag
     1741 ggcatagtcc gggtcgatcg accaggccgc cgtcctcgcc ccccggagcc caacctcttc
     1801 ctgggcggag aacaccagat acaggtcgtt gggacaggcg tccaactcct ccagtacccg
     1861 caggagcaca gcgcaggaga tacggttatc caagtaaggg gaaaccactt tatccccact
     1921 caaaaatatg gcggtatcat agaccgcggt gtctccaacc tggaccatcc tgcgggcctg
     1981 ctcctcgtcc ttcgccccga tgtccacata gcaggcgtcc agcttcagct tgccgaattc
     2041 cgccttctcc tccgggacga tgaccccccg cactccgttt ttgaagcgca ccggggtgta
     2101 cagcagctcc cgggcggcga tcccgcccag ggtccccacc cggaggaacc cctccttctc
     2161 gatgtgggtg acgatcaggc cggtggagtc catgtgggcg cacagcatca ccttgggacc
     2221 gctcccccgc ttccggacga tcaggttccc cagggtatcc gccgtcacct cgtcggcgtg
     2281 gggccgggcc agggcggcaa tgacctcccg caccgcgccc tcgtccccgg aggggccatg
     2341 ggcagtgttg agggtctgga tcaattcaaa cagttccatg ttacaactcc ctaatcgtta
     2401 cagcgtcagc tgggccgcct gatcggccag gaacagccgg gtcagcttca gcatggcttc
     2461 aaaatcgaaa atgctgccca cgctggccgg ggcatggaga tagcgcacgg cggcggacag
     2521 ggcggccacc cgcacgccgg acttggtgcg ctggatggtc cgggcgtcgt tgcccccggc
     2581 aatatattcc ttggtctgcc aggggatccg gtgctcctcc gccagacggc gcaggtcctc
     2641 gaacagcccc cggtcataga tggtcccccc gtccatatag gagatcaccg ggccttttcc
     2701 cggggcgcac accttccggt gagcctccac catgggcagg tcggcggcgg tggtggtctc
     2761 cagcaccaga gccacctccg gcgtgacgga gaaggccgca ccgaaggcac cccgggtgcc
     2821 cacctcctcc tgtgcggtga aggcgaaggt cacgtccatg gggagctttt cccgcagcag
     2881 ctccagcatc acggcgcagc ccacccggtc gtcgatggcc ttggccttga acaggccctc
     2941 cccaaagcgc tccggcggtc ccacaaaggc cccgcaggtc ccaagtggga ccatggagag
     3001 ggcctcctcc cggctggacg cgccgatgtc caggtacagg gcgtcggtct tggggacctt
     3061 cttcatgtcc tcccggctga gcaggtggat ggccttcatc ccgatgaccc cggggacccg
     3121 gccgggcccc agctccaccg gcttgcccag ggctacccgc cggtccacgc cgcccacaaa
     3181 atcaaacttc aaaaagccct cgtcggtggc ccgggtgacg atgaccccca cctcgtccat
     3241 gtgggcgcac agcatcagcc ggttttccgc cgccttccgg ccccgcttga acacgatgag
     3301 gttgcccagg gcgtcggtgc ggacgctgtc cgcgtgctcc tccgcccagc cgcggatata
     3361 gctccgcacc tgtccctcgt cccctgagac gccgttcagg gcgcacaggg tctccaaaca
     3421 ctggatcaaa gcgcctccgc ctcctctcca aggccctcga gaaaggcggc cagcagctgg
     3481 gccgtgtagt ccaggtcgtc ccggtggacc gtctcgatgg gggtgtgcat ataccgcagg
     3541 ggcagggaga gcaccgccgt ggcgatcccc tcccggctga tctgcatggg ccagccgttg
     3601 gttccggagg agccggccat gacctccagc tggacgtcca gccccagctc ctccgccttc
     3661 cgctccagcc gccgggccat ccagcgggtg cagttgggcc ccagcccgat gaccgggccg
     3721 ccgcccagag ggaaggtctt gtccttggat gcgtccggtg agtccccgtg ggtcacatcc
     3781 acggccacgc agcagtccgg ggcgatcccg tatgcggcgg cgatggctcc ggtgctgtgg
     3841 gtctcctcct gggtggagcc caggacatac aggtccacat ccagctgcct gtcccgcagc
     3901 cgctccaggg cgtccagcag gatggcaaag caggcccggt cgtccagcgc ctttccgcac
     3961 agcagcttgt cccccagagg agcacagccc gcccggtaga ccgccggtgt gcccacaggg
     4021 atgcgggcct gggccgtctc ctgatccagg cccgcatcca gatacaggtc ctggatggcc
     4081 acagattggt ccatatcctc ccggctctgg atgtgggggg gcagacagga caccaccccc
     4141 accacagggg gctgggtgag gatggtcagt tcccggtcag ggagcatcct ggggtccacc
     4201 ccgcccaggg gggcgaagcg gaggaagccc tcctcatgtc cggtgatgag aaagccgatc
     4261 tcgtccagat gggcgtccag caacaccttt ttggccccct cccggccaca gcgccgcacc
     4321 cccaccacac tgcccatgcg gtccagaaac gcctcgtcca ccagggggcg gagcagctcc
     4381 agagccgccc gggccgcctg gccctcaaac ccgctggggc cgggcagggc gcacagcccg
     4441 gccagcgtct tttcatagtc caaacaaatc ctcccgtctc tctgctcaga gggcctggac
     4501 caggcgctta aaggcctggc cccgctccat aaagtttttg aactggtcaa aggcggcgca
     4561 ggcgggggac agcaccacca catccccctc ccgggcggtc tcccgggcgg cgcgcacggc
     4621 ggcggccagg tcctcctcct cccggatcac caggccgctg gcctcaaagc tcggggcctg
     4681 ctccgccgca ctgcggatgg cggaggcggt gtccccggtg aggatcaggg cctttacccg
     4741 gtccacgatc tccatgccaa gctgggtaaa gggaattccc ttgtcgtagc cgcctgcgat
     4801 cagcaccacc ttccggtcga aggactccag gcaggcggtg gtgcggctgg ggctggtgcc
     4861 gatggagtcg ttgtaccagc gcactccgtc cagctcccgg acgaactcga tgcggtgctc
     4921 cactccctgg aagcgccggg ccaccgcccg gacgcagcgg tcagggacca ggccgtccac
     4981 cgcggcgatg gcggccatat agttttccac gttgtggtcg ccggggagct ggatcagctc
     5041 cagaggcagg acctcccggc tcccctggtc gttggtcagc cagatggccc catcccggag
     5101 gtataccccc tcctccagcc gctcctgccg gctgaagaag gtcacggggc acaccgcctc
     5161 ccgggccagc gcccgggtgg gggcgttgtc atagttgagg atcagccggt cctccggccc
     5221 ctggtggagg aagatgttct tcttggcccg gacgtactcc tctatggtgt ggtggtagtc
     5281 caggtggttg gggctgaggt tggtgaccac cgcgatatgg gggctctgct ccatggtcat
     5341 cagctggaag gaagacagct ccagcacggc gaagtcctcc tccaccatgt cggccacgtc
     5401 cggcagaagg ggctttccga tgttgccccc cacatagacg ttccgccccg cctccttcaa
     5461 aaactcggcg atgatggtgg tggtggtggt ctttccatcg ctcccggtga cgccgatgag
     5521 gcggctgggg caggtctgga aaaacagctc catctcagag ctgagctcga tgcccctctg
     5581 gacagcgttg accagctccg gcgtgttggg ggacaggccc ggggtgcgga agatcacatc
     5641 ctcgtggagg tcctggaggt actcctcccc caggatcatc ctggccccca ggctctccag
     5701 ctcctggacc tgctccgccg cccgctcccg ggggacccgg tcccggacgg tgacctctgc
     5761 cccggcccgc agcagcatct tgatcagggg cgtgttgctc acccccatgc caatcaccgc
     5821 caccgggcgg ccctgtattt cctccagata ttccttgatg gtggacatag cgcgtcctcc
     5881 ttccatcgtg ttccgggccc cgccgggacc cgtgcgcttt taaatccggc cccgccggcg
     5941 gaccggcggc agtctatata actaaaatat attaccatag ccggaaaaaa aattcaacga
     6001 acatttgaca ccggaggcgt tttcctgtat gatagacggg caagcaggct ggacagccgc
     6061 gcgggcgggg cgaaaggccc gtctgtgagg aaagtccggg ctccataggg cagggataac
     6121 ggataacatc cgccgggggc gaccctagga aaagtgcaac agaaatagtc tgccggcctt
     6181 cgggccggtc aaggtggaaa ggtgaggtaa gagctcaccc gacggcgtgg aagcgcccgt
     6241 cgctgtaaac tctatccgga gcaacgccgt ggagggacac acaggtcggc ccgaccgtcc
     6301 cggggaggcg gctggagcgc gccggcaacg gcgcgtcgag atagatggct gtcttaaaag
     6361 acagaacccg gcttacaggc ctacttgcac caaaaggacc ggtatgagaa ctcataccgg
     6421 tccttttctc atcgctgcat ggggtttatg cacccccgct gcggatgcgc tccaccaggc
     6481 ggcgggtctg ctccagatcc gcgctgtacc gggcgtcggg cggctctgtc cgggcacggg
     6541 gcgcgggact gctgggggcc ggagcgcccc gctccccctc ccgggtctgc tggcggcggc
     6601 gctctccggc ctgacggcgg aggagccacc gctgggtgtc ccggtccacg gcggaggcca
     6661 tgaagcggaa gcccatctgg gcctgggccc ccatgtccgg ccagcggacc agcacttcct
     6721 ccgggtccgt cacctcccca cgccaccgcc cgtcggcata ctgcatcagg gcggtaaaca
     6781 acaggccccg ctgctccggc ggcatcgtca gaagcattgg gtagttgtca aaatagacca
     6841 caaagccccg ttttggattg ttcatgtcca ttcctccttt tacccctggc gggaaccgcc
     6901 ggtttggtgt acatttgcgt gccactatcc cctccgtaaa gggtaatagt aaaagtcata
     6961 gaaaaaagta atggtcactg tgatagtcag gtcaagcctg acggcggcgg cccgggcccg
     7021 ggccggagaa aaaaataaaa aattttttct tccccgcaat aaaaccccgg tcccgtgcat
     7081 ttcatagtca gaagcacaaa ccagagcagc ctgaacggct gagaaagaga ggaatccaac
     7141 atgaaaaagt cttacattgc cgccgccctc gccctcacca gctgcatggt ggtggggacc
     7201 gccgccgcca gccagagcaa cagcgtgacc gccaccttcc gtcccgacat gacgctgaag
     7261 gtcaacggca ctgcctacac cgtccgggac accaccgggg tgaaggtctc ccccctgatc
     7321 tacaacggca ccacctacct gcccctgtcc tccctgggtc agctgctggg ggcggaggtc
     7381 tcctgggaca acgacagcca gaccgtgatc atccacgatg atgacgccga ctacatcggc
     7441 gaggccaaag ccaaggaact ggccctgaag cacgccggac tctccagcaa ggaggtctcc
     7501 ttcctccagc tgaagctgga ctgggacgac ggccgcgccg tctatgaggt ggagttttat
     7561 gccggcagcc gggagtacga ctatgagatc aacgctgtga ccggcgcggt ggtggacttc
     7621 gactccgaca tcgaggacta caccatcccc tctgacagcg aggactatat ctcccgggag
     7681 aaggcccaaa agctggccca ggccaaggcc ccgaaggcca cgctgatcaa gctggagctg
     7741 gatgtggacg acggccgcgc cgtctatgag ggcgagctgc gggaaggcag cacggaatat
     7801 gagtttgaga tcgacgccgt caccggcagc ttcctgaagt gggagcagga gcgggactga
     7861 agcccgcccc catatcaaaa tagacataca aaagggaccc gccgccggcg ggtcccttgt
     7921 tcgcttcctt ccggcaggcc gggcgtcacg gcttttcggg ctccagttcc ttccacagcg
     7981 cccgcagggc ctccagctcc gcccgggcgc cgtagtcctc cagctcgccg ctctcctccc
     8041 agtcccggga gaggatctct tgggccaacg cggaagcctt ctccctatcc ttccgggtcc
     8101 caatccccag ggcatagcac cgcgccaggc ggcacaggct gttcaggtcc tcccgttccg
     8161 cgccctttcg gtaataggca aatgcctgtt ccagatcctg ctccacggcg gtcccggtct
     8221 cgtaaaaccg gcccagcccc cagcaggcgg agtcatctcc cagctcatcc gcccggagca
     8281 ggcactccag agcctggtcc agctcccccg cctgctcgtg gtaccagccc aggttgtacc
     8341 aggcgcactc atcatccagc tctgccgccc ggcggtacca ctccatagcc tgctccaggt
     8401 cctggggaac gccatccccc ttggccagca gctccgccag gttgttcatg caggccgtgc
     8461 tgcccgcctc cgcgccccgg cgataccaat ccgccgcctg agccggatcc cgctccacac
     8521 cgagcccctt ctcatagcac caagccagtt ggaacatagc ccgggtgctg cctttttcca
     8581 cgcccttgcg gtaccagcag aaggcctcct cccagctctg ctccacgccg ttcccgctct
     8641 catagcacca gcccaggttg cacatcgcct gggagtcgcc cccttccgcg ccccggcggt
     8701 accagtggac ggcctcctcc cagctctgct ctacaccgtt cccgctctca tagcaccagc
     8761 ccaggttgca catcgcccgg gcgtcgccct cttccgcgct ccggcggtac cagaggacgg
     8821 cctgctccca gctctgttcc acgccgtttc ctgtctcata gcaccagccc aggttacaca
     8881 tggcctgggc gctgcccgcc tccgcgccct tctcatagca acggaccgcc tgctccgggg
     8941 cggcctgggt cccgacgccg ctgagcagac agcctgccag gagcagcagg ccgcgttctg
     9001 aaccagcttc ggccgcctgg cggtagagct ccaccgcccc ctccgggtcc cggtccgccc
     9061 acttgccgtg ctccatcagc cagcccagac agcattttgc ctccggctcg ccgccctccg
     9121 ccgcccggcg gtaccagcgc accgacagct ccgggtcctg ttccaccccg tcgccgaact
     9181 catagtgctt ggccagcagg gtctggcaga ccgtgtcccc ctgaagggcc gccatgcgca
     9241 gccactccac cgcctgggcc gggtcccgcc ggtccatccc gatcccccgg tcacagcagc
     9301 gggccagcag gcgctgggcc cgggtggagc cgtactccgc cgcccggcgg tacagctcca
     9361 ctgcctgacg ggggtccgga gggatcccga tgccctcctc atagcacacc gccaggttgc
     9421 acagggcggg caggctcccc atctccgccg cctgctggta gagccagact gcctgacggg
     9481 ggtcggcctc tatcccagtg ccctgctcca ggcacaggcc caggttgcac gctccccagc
     9541 cgctgcccag ccgggcggcc ctgcggaagc aggacacggc cttctcctcc gccccgtcct
     9601 gttccatcca gaacaccccc agggcgcacc agtcgtcccc ggacatcttc gcccgctccc
     9661 ccgcggtcag cgccccgccg caccgggggc aggcgtccgg catggccgcc ccctcgcccc
     9721 agaagcggca gtctcccttg gtacagtgaa agagcatacg cccctctctc ctctctgatc
     9781 tgtctgatgc tgcggggctc agtccagccc ctgctccaaa atgcggtaca ccgcctccag
     9841 gtccaggctg gcccgcagag cggcggccag cttgtcatac tgctcctccc gataggcctg
     9901 gccgtcctca tccccggtct cccccgtcca ggtcagcccc ctccgggcca gcagcaggga
     9961 gaccagccgg tcccggagct ccggtgtgtc aaaaaagccg tggagatagg tgcccagcac
    10021 gttctcccgg acggaaaaca ggccctccgg tccgctgtcc cccatgtgga tctcatagcc
    10081 ctccaggggg cagccctcca ggggggcgaa caggccggtg caggccggga aggccgccct
    10141 gcggcgggtg cgggtctttt cccgggaaaa gcgggtggcg acgggcagca ggcccagccc
    10201 ctcctgggag ccgcctccct ccgccccctc cgggtcgtcc acccgctgtc ccagcatctg
    10261 atagccgccg cagatcccag ccaccggggt ccccgccgca gccagcgcca gcaccgcccg
    10321 gtccagcccc cgccgccgca gccaatccag gtcggccagg gtgctcttgg tccccggcag
    10381 gatcaccagg tcgggccgcc ccaggtcctc cggccggtcc gtatagcgca ggcccaccgc
    10441 cggatgccgc cccagggcgt tgaagtcggt gaaattggag atgtgcggca ggcggaccac
    10501 cgccacgtcc agaagggcct cccgccgccc ggaggtgagc cggggagaga gactgtcctc
    10561 atcgtccaga tccagctcca gccaggggac cacccccacc accgggatct gggtcagctc
    10621 ctccagcatg gtcaggccgg gccgcagcag ggccgcgtcc ccccggaact tgttgatgac
    10681 cgtccccttc accatggccc gctcgccggc ctccagaagg gccaccgtcc cgtacaggga
    10741 ggcgaacacc ccgccccggt cgatgtcccc cgccagcagc accggggccc gggccatccg
    10801 ggccatcccc atgttcacga tgtcgtccgc cttcaggttg atctccgccg ggctgcccgc
    10861 cccctccagc acgataacgt cgtgctcctc cgccagggag cggtaggacg ccaggatctg
    10921 ggggaccagc tcctttttat ggcggaagta atcggacgcc cgcatatccc ccagcacctc
    10981 gccgttgacg atgacctggc tgcccatgtc gctgctgggc ttgagcagga tggggttcat
    11041 tcggacgctg ggctccaccc cggcggcctc cgcctgcatc acctgggccc ggcccatctc
    11101 caggccctcc cgggtgacga agctgttcag cgccatattc tggcttttga atggggccac
    11161 cgaatagccg tcctggcgga agatgcggca cagccctgct gtcagcacgc tctttcccgc
    11221 gttggacatg gtcccctgga tcataatggc ctttgccgcc atgtcagtcc ctcctcagct
    11281 gccgcgccgc ggcggtcagc agtctctcgt tctcctcctc cgtccggaca ccgatccgga
    11341 accagccggg ccccagcccg gagtagtcgg agcagtcccg caccaggatg tgctccctct
    11401 ccagcagccg cctgcccagg tcctcaggcc cccgaaacag gagaaaattc ccgtcccccg
    11461 gacacaccca gaggcccagg ccggtcagcc ccgccgccag ccgctcccgg ccccggcgga
    11521 tcgcccccaa actgtctttc ggccagcggg agaactgctg taaagcgatc tcacctgccg
    11581 cttgtgccgg gacagacacc gcccagggcg gccccgccgc cgccatccgg tccagcagcg
    11641 cctcctctcc gcacagggca tagcccaggc gcaggcccgc cagggcatag ctcttggtca
    11701 gcgcccggag aagcagcagc ccgcccccgg acagctcctc cgtcaggcgg cgtggatcgc
    11761 cgccggtgag ctccagaaag cactggtcca ccgccagaaa ggcccccacc tcccggcagc
    11821 gggacagcac ccggcccagc agccccccgt ccatcagccg cccggtgggg ttgttggggt
    11881 cgcacaggaa caccagatcc accccgggga cgatggcctc cagcaggtcc tcccccggca
    11941 gaaagcctgt ccgctcctca agccggtgct cccggatctg gcagcccacc ccctccagag
    12001 cccggcggta ctccgaaaaa gtgggggcaa gtaaaagtgc ttgacgcagc ttacaagcca
    12061 gggccatgcg gtagatcaga tcggaggccc cgttgccgca caggatctgg tcctcctcca
    12121 cgccgtcccg gcgggcgatg gcccggcgca ggccgcggca ggccgggtcc gggtagcggg
    12181 cgcagtccag caccgcccgg caggccccct ccagcacagg accgggagcc cccatggggt
    12241 tcaggttgat ggagcagtcc aacagatccg ggggacagcc gtaggtgtcc cctccgtgtt
    12301 caaagcgggc catgtccgca ccttccttct ctgtgatgtt aatggagtgc ttttttatgt
    12361 agggcgcaac gactcggtgc gccgcctcaa atggcgaaac cccagcgttg aatcaagaaa
    12421 ttccgaaaac cggcgggagg ggcaagctcc tcccctacgg gatagcgggg gaaatattct
    12481 gtggcggaga accggcgggc gggccggtgt cccgccctac ggggcaattt cggttatgcg
    12541 tgatatggcg gccgccgatt ttcagaacgg tttccatatc acgccgccag cagggtcccc
    12601 caacccagca ccgccgccag ggtcagcagc tccgtgacat acatcagacg gcaggcccgg
    12661 gcgacatccg cccgctccac cggccggagg ccgtccccaa tggtgggctt ggacaccttt
    12721 ttcccaaaat accaggcgtc ccccgccagc tgcactccca gggccccggc gcaggccgcc
    12781 tccgtgtggg cggagttggg ggagctgtgc tttctccggt cccggaaaaa gaccctcagc
    12841 gcccgccggc cggagcagcc gggcagcagg gcggcggcca gacacatcat caggccggac
    12901 agccgcgcgg ggatccagtt gagcacatcg tccagccggg cggccgccgt gccgaaccag
    12961 cggtaccgct cgttccggta acccaccatg gagtccatgg tgttcaccgc cttatagacc
    13021 atccccaggg gcagcccccc cagagccaga aagagcagcg gagcagtcac gccgtcggag
    13081 gtgttctccg ccaccgtctc caccgccgcc ttcaccaccc ccgcttcatc cagccgctgg
    13141 gtgtcccggc ccacgatgcg ggagaccgcc tcccgggccc ccgccaagtc ccccgccgcc
    13201 aggggagggc ataccgccat gctctctttc tggagggccc gggcggccag cagctgatag
    13261 gtgaaccaga cctccaccgg ccaggccaga cagggggaca gccccctcag cccctccagg
    13321 agcagccagc tgcccccgcc gaaggccgcc gggatcagca gggccagaag ggcccccgcc
    13381 agccgctccc ccgccggggt cccagggaac acacggcgca ggacgccctc cagccaggta
    13441 atggcccggc ccatccaccg caccgggtgg ggcatccagt gggggtcccc cagtagccag
    13501 tccaggacca ccccgggcag caagacggtg attagatgtg ctgtcaacgt catagcgcct
    13561 ccagcacccg cagctccagg ggttcccgcc ggacctccac ccgccagccc cggcagttct
    13621 ccggctgcca gcggtagagc tcccactccg ggcggccaaa ggcggacatc accgccagcc
    13681 aggtgccccc gtgggccacc acggcccccc ggtccactcc ggcggcctcc gcctcccgga
    13741 ccacggcctc tatcccccgg cggcttcgct ccagaaagcc ctgcatgttc tctccgccgg
    13801 ggaaggtgat ggtcaggtcg ccccccatcc aggcccggta caggggatcg tccttcagct
    13861 cctcccaggt cttttcttca aaccgtccga aggcgcactc ccgcagctgc gggacctgcc
    13921 gctgctccac cccggggaac agcagcgccg ccgtctgacg gcaccgtagc atgggggaca
    13981 cccacagccc gccgatctcc ggcatctccc cccggcgctt ccgggccagt tcctccccct
    14041 ccggggcaag gggctggtcg gtggagccga tatactgtcg tttcaaattt cccggcgtga
    14101 tgctgtgccg gagcaaaagc aattccatgg cgttcccgct cctgtctttc tctactgatc
    14161 acgaggcccc cagcttcttt acaccggaaa cccccgcaag tgttttcact ttatgaaatt
    14221 tgtcatttca gccgcatggc aaggccgcac tggaggcggt agacggcctc cgcctcccgg
    14281 gccaggtcac agcacaggcg gcccaccgcc tcccgccggt cccggtcccc ccggtccatc
    14341 gggaccacgc cgcagcccac ctcccggcag accaccaccg cccggcccag cagggcctcc
    14401 agaggcggga ggggatccag ttcctccagg ccgtacagca ccgggccgtt ctccgcgaac
    14461 tgctccggag aaaatcccag cacgtccgtc acaaagctcc tctttccggc cccgcggccg
    14521 ccgatcacaa gaatcactgt actccctcca aactctgggc cagcaccaga ccgaccaggc
    14581 aggccagctc acacagctgt aaaaaccagc ccgccagatc gccggtggtc ccgccaaatt
    14641 cccgctttgc cgttttttca tagtaccaca gggccagcag ggccgccccg ccgatccaca
    14701 gataccccgg cttcaaaacc gccagtccgg cggcgcacag cagggcgccg cacagtggga
    14761 gccagcggcc ccggcggcgc tccccggtca ccgccgccag caggcccccg ccccgggcgt
    14821 tgggcagggt cagggcggca aaggcggaca gcccccggct gagcaccggg accaggagca
    14881 gggcggccca caggcccccg tccgtccgct ccgcctgaca ccacagcccg aaggtaagca
    14941 gcagcagcag cccgcagccc tgcaccgcaa aggcccccac atgggggtcc gccatgatct
    15001 ccagcttccg ctcccggctc tggtgggagg ccagggcgtc gcacacatcg cagaagccgt
    15061 ccagatggat ccccccagtg atcagcaggg ggatggcggt catccccgcc gcccacagca
    15121 gctgccccag cccaagggct cctgccagcc aggtccacag cagcagtccg ccgcccaccg
    15181 ccagccccac cagagggaag gcgtacaggg tgtatcgccg ggccccgctc tcccagtcca
    15241 gagggggcac agggatgcgg gagtacaggg cgaaggccat gcccacggcc cgcagaaccg
    15301 tcatacctct ccccccttcc agtggacggg cagtccgcag accagctcgg tcacccggtc
    15361 cgcccgggcg gccaggtccc ggtggaggcg ggccagcagg tccagatagg ccccggtctc
    15421 cggcgggtag tccaccccgt cccggaacag gtcgccgctg accaccacca gatgccccgc
    15481 tccccctctc aaacgatcca gccccctccg gatgcggcgg tacgccccct ccgggtcgaa
    15541 ggctcccccg gaaaacagct cgttggccgc caggttgccc agatcctcca gcaaaacggc
    15601 gctccccgcc ggcggggccc atcccgccag atcccgggga cgctccaccc tacaggaaaa
    15661 acccttcccg gcccggaggg ccttgtgacg accgatgcgc gccgccgcct cgccgcccca
    15721 gggctccatg gtggccagat agatcagggg
//
LOCUS       DBMDSJ010000004        15475 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_1418_length_15475_cov_2.425263, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000004 DBMDSJ010000000
VERSION     DBMDSJ010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 15475)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15475)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15475
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1418_length_15475_cov_2.425263"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /gene="ligA"
                     /locus_tag="ACZPAQ_00280"
     CDS             <1..1583
                     /gene="ligA"
                     /locus_tag="ACZPAQ_00280"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR0902438.1"
                     /translation="RGDGQVGEDVTENLRTVRSIPLRIPDAPARLIVRGEVYMPKKVF
                     HALNEERERRGEALFANPRNAAAGSLRQLDPRVAASRRLDIAVFNVQWAEGETFRTHV
                     ETLEYLKQKGFKVIPHYSCRGVEDAIGQVAAIGEGREDFPFDIDGAVIKVNDLAQREL
                     LGSTAKFPRWAAAYKYPPEVKSSRVLDIVIQVGRTGVLTPRAVLEPVRLAGTTVTSAT
                     LHNQDFIAEKDVRIGDTVLVRKAGEIIPEVLSVELEKRPEGAEPYRFPQTCPVCGAPV
                     ERDEDGAHIRCTGAECPAQLLRNLAHFASRDAMDIDGLGIAVVENLVNAGLVKTPGDL
                     YFLEAGKVAELDRMGKRSAQKLLEAIERSKTRDLSRLLFAFGIRQVGQKAGKVLAARF
                     RTLDALQGATLEELTGVDDIGAITAQSLLDWFASPQSQHLIGRLREAGVNMEAEEQGS
                     DQRFAGMTFVLTGSLERFTRDEASKMIEDRGGKVSSSVSKKTSYVVAGAAAGSKLRKA
                     QDLGVPVLTEEEFLALMD"
     gene            1671..2615
                     /locus_tag="ACZPAQ_00285"
     CDS             1671..2615
                     /locus_tag="ACZPAQ_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR0902439.1"
                     /translation="MKKGYLYIIVTIVMFTSYEVVLRYIAGQINSMQLTLCRFSVGFL
                     ILLPLALRTLKRRGKRLDGRSMARFALLGLLGIALSMPILHLSVRYAGSSVAAVLFSC
                     NPVFVTFLAFFLLKEPIKPRHISALALEIAGTVAIIDPLGHALNPLGVGLALLSTLLF
                     SLYGVLGKRRVAEYGGAVVTCFSFLFGSLIVLGLILLSHVPAVAQGMTAAGLEEFARI
                     PILAGYTLENLPYVLYVSVGVAGIGFCCYFLAMEQLPASVVSLVYFFKPALAPVLAWQ
                     LHGEAIPANMMAGILLIVAGSLCTIIPGLLDARRKQRA"
     gene            2678..3808
                     /locus_tag="ACZPAQ_00290"
     CDS             2678..3808
                     /locus_tag="ACZPAQ_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR0902440.1"
                     /translation="MSVSKVYFTDFHTKAFGDGLPTKLKKLIRAAGIQKLDLDGKFVA
                     IKMHFGELGNVSFLRPNYARAVVDVVKELGGRPFLTDCNTMYPGSRKNALEHLECAWQ
                     NGFTPLTVGCPILIGDGLKGTDDVEVHVAGGEYVEKAKIGRAVMDADVFISLTHFKGH
                     EMTGFGGTIKNIGMGCGSRAGKKEQHASGKPKIDPEACRGCRRCQKECANNGLVFDTE
                     TGKMTVDQDSCVGCGRCLGACNFDAIYFTNDNAQAALNCRMAEYTKAVLDGRPNFHIS
                     LVVDVSPNCDCHGENDVPILPNLGMFASFDPLALDQACVDACLAAEPMPGSQLARHLA
                     EPGFHDHHDHFTNSTPESEWRSCLEHAAKIGLGSREYELIRL"
     gene            3874..5310
                     /gene="purB"
                     /locus_tag="ACZPAQ_00295"
     CDS             3874..5310
                     /gene="purB"
                     /locus_tag="ACZPAQ_00295"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR0902441.1"
                     /translation="MPTNTYESPLSSRYASDEMLYLFSADKKFSTWRRLWVALARAEM
                     ELGLPVTQEQVDELEAHITDIDYEKAAQWEKKLRHDVMAHVHTYGELCPKAMPIIHLG
                     ATSCYVGDNTDVIIMREGLELVRNKLVQVLSRLADFAREYKALPTLGFTHFQAAQLVT
                     VGKRATLWMNELLMDLEEVEHRISTLKLLGSKGTTGTQASFLELFEGDHEKVKELERR
                     IAEELGFDAVVPVSGQTYSRKMDYNVLSTLAGIAQSASKFATDLRLLCHLKEVEEPFE
                     KNQIGSSAMPYKRNPMRCERICSLARYVIVDAGNPAITTATQWFERTLDDSANKRISI
                     PEAFLAVDAILNIYMNVASGLVVHPKVIRRHVMEELPFMASENIMMDAVKRGGDRQEL
                     HERIRVLSQEAGRNVKDLGLSNNLIDLMAEDPAFGMSREELTAHLEPERYIGRCPEQV
                     EEFLSQEIAPVLEKYAAALGGKETELKV"
     gene            5330..6631
                     /locus_tag="ACZPAQ_00300"
     CDS             5330..6631
                     /locus_tag="ACZPAQ_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR0902442.1"
                     /translation="MKHQKYGAYLAAGVTAFAVIAAAILLFFVIYHFSAVRALLELLA
                     YILRPIFYGLVLAFLLLPVHRQIYSFLAALCEGVAERGGRKRAALNFLAIVLSLIFAV
                     LVVYVLLAMVLPQLYLSIVGLVNAVPEYIRIMQQWLLDFLADNPEIQTAVLPYYNSLA
                     QSVEQWLSSDMLPNLESVNSTLDWLKAEILPQLTGVVSSVSSGVVALALLLKDILIAI
                     IVSVYLLARKDVFAAQSKKIVYSVFRTDVADLLVEETRSAYRILSGFINGKLVDSLII
                     GIIALVCCNLFRFPYPMLLAVIIGVTNIIPFFGPFIGAIPCALLILLVSPLKCLYFVI
                     FIFVLQQFDGNILGPKILGDSTGLASFWVLFSILLFGGLFGFVGMVLGVPVFAMFYSV
                     VSRLVRCGLKKRGLPLETEQYLGRTGPLAGTGPGVEDSGTR"
     gene            complement(6758..8002)
                     /locus_tag="ACZPAQ_00305"
     CDS             complement(6758..8002)
                     /locus_tag="ACZPAQ_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR0902443.1"
                     /translation="MKQLTGACIIGQSGGPTAVINASAQGVIQTALKADCITHVLGAA
                     HGIKGVLEDKLYDMGQEDSGELDLLKFTPSSALGSCRYKLADPDVDDADYRRILEIFK
                     KYDVRYFFYNGGNDSMDTCNKISKYMQKVGYECRIMGVPKTIDNDLNGTDHCPGFASA
                     AKYIATSCAEVWQDAHVYDTGMVTIIEIMGRHAGWLAGSAALASLTGCGPDLVYLPET
                     DFDMERFLADVKAVYDQSGNCLVAVSEGIHYADGTFVSEAKTSATDGFGHAQLGGLAS
                     HLASAVKEAFGVKVRGIELSLLQRCGAHLDSETDAEEALLAGSAAVQAAVEGFTDKMV
                     AFRCTRGEDGYRCETILEPLDIVANFEKKVPREWINEAGNGVNQQFIDYVLPLIQGDA
                     KAPRVNGLPRFARLKKVLTTQL"
     gene            8148..9116
                     /gene="fba"
                     /locus_tag="ACZPAQ_00310"
     CDS             8148..9116
                     /gene="fba"
                     /locus_tag="ACZPAQ_00310"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HIR0902444.1"
                     /translation="MPLVTTTEMFKKAYDGGYAIGAFNVNNMEIVQGITEAAQEEKAP
                     LILQVSKGARAYANHTYLVKLVEAAVIECPDIPIALHLDHGPDFETCKACIDGGFTSV
                     MIDASSKPFEENIAITKKVVEYAHDHGVVVEAELGTLAGVEDDVKVSAEDSSYTRPEE
                     VEEFVTRTGCDSLAIAIGTSHGAYKFTPAQCTRNEKGILVPPPLRFDVLEEVSKRLPG
                     FPIVLHGSSSVPQEYVKMINEHGGKMPDAIGIPEEELRHAAELSVCKINIDSDLRLTM
                     TGTIRKFFEEHPDKFDPREYLKPARANIKDLVRHKLIHVLGCAGKA"
     gene            9248..9919
                     /locus_tag="ACZPAQ_00315"
     CDS             9248..9919
                     /locus_tag="ACZPAQ_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902445.1"
                     /translation="MDRHFFFLAYLTANKQQRRWLVIIWAVVPLLFALAMFLTLSPYR
                     GISYQYQESYPIVGTENRQLWTLKGSELVTLFNENLPDTAPELSYLHEPTPSDRQIML
                     TDNGKTWSIVFRQVPEDAASIYFWSKQPEIDAWLGNVEDVKLSLYHTSAQDILLNEQY
                     ARCLINIFTPGAEDYVVRRLHLSRPLTSGYKRVKTGDVLYTHKGGTSPVLIIEPDCRN
                     WPPDR"
     gene            10115..11164
                     /locus_tag="ACZPAQ_00320"
     CDS             10115..11164
                     /locus_tag="ACZPAQ_00320"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR0902446.1"
                     /translation="MKIVVLAGGLSPERNVSLSSGAMVCEALRSKGHQVALVDLFFGM
                     EGTDQTVDPFQAPIPQAFKKVSPQAPDLEQVRASRSDRSPSLIGPGVLELCAGADVVY
                     LALHGACGEDGRIQAALDLLGVPYTGSNCLSSAIAMDKDMTKRLVADQVRTPRWQTVT
                     VTADNLDVLTEETALPAVVKPIASGSSIGVTIAHTREELRRGLEESVRLGGRTVLEQY
                     IKGREIQVAVLNGRALPSIEIIPQADFYDYANKYQPGAAREVCPAEIPPEWEKAVGEA
                     ALAVFRTIGLSVYARADFIVTEDGTPYFLEINTLPGMTPTSLVPQEAAAVGIGYADLC
                     QTIVEASLEARREGV"
     gene            11171..12565
                     /locus_tag="ACZPAQ_00325"
     CDS             11171..12565
                     /locus_tag="ACZPAQ_00325"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR0902447.1"
                     /translation="MEAITVRDLLTAVGGTLIGPETSLDRTVSSVDTDSRDIHPGSLF
                     LPLVGERFDGHTYIGSALEAGAAGCLTAREPEHYQEGRFYIKVEDTQRALRDLAFWYK
                     EQFRIPFVGVTGSVGKTTAKDMIASVLSVKYKVLKTEGNFNNNVGLPLTLLRLDRSVQ
                     IGVVEMGMDRFGEIDYLGDIVRPEVGVITNIGDAHIERLGSRENIFRAKCELLPHIRK
                     ENGLLILNGDDALLSTLRGRTPVPAVFCGSGEGLEYRAQSASSEGLDSLRSRLTTPAM
                     DREVCIPALGSHMIYPTLIAAAVGEHFGLTADEIEQGIARFVPTRMRMNLVQRGNGIT
                     ILDDTYNANPQSMRAAISVLADSRSAWKLAVLGDMFELGPYSPALHTGVGEYLGKRKI
                     DCLVAVGDQARYLAEGAQSAGMTAVYPCADKKAAREILPGLIRPDSTILVKASRGMRM
                     EELVDQLLELTPEQ"
     gene            12626..14545
                     /locus_tag="ACZPAQ_00330"
     CDS             12626..14545
                     /locus_tag="ACZPAQ_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR0902448.1"
                     /translation="MIDISVSNLVKEFEVGTKILDGLTFQVDTGERVGLLGPNGCGKT
                     TLLRILTGAMDYDEGEVMIAPGKRMGLISQIPVYPEGYTVEDVLATAFEPLRKMEDEL
                     ARLTREMASGTDPALMSRYDKLTTAYEAAGGYETDTRINKVCNGLSIPQAMREQLFES
                     LSGGEKTRVNLARLILEDTDILLLDEPTNHLDLRATEWLEEYLGKFKGTVLTVSHDRY
                     FLDQVVDRIIEIQEGRAEFYQGSYSFYAVEKERRYEEKLKQYEKEQAKIEQLEKAAEQ
                     MRVWAYSGMDKTFKRVKSMEKRIERMRVTDRPKKERKMEVRFGEREFRGDEVLTIKGL
                     TKGFGGRTLFSGLGLEVAGGERIALIGDNGSGKTTLLKMILGEEAPDGGKVRMGPTVK
                     VGYLPQHVHFDHPERSLVDTLIYAQDCTAQSARNRLAAFKFRGEDVFKPVSALSGGEQ
                     SRLRLCMLMDEKINLLVLDEPTNHLDIQSREWIEEAVEEYEGNLLFVSHDRYFIDRFA
                     SRIWLLENGQITDFCGTYQEFQAARARGAAGQAASDTVPAPAKAAEPERKKEKPKRPG
                     GTKNLEKEVTAAERAVAKAEEAMDALSLQIEEASADYLKLQELYEQREALEAELQGLY
                     DRWETLSAQLEEARG"
     gene            14552..15175
                     /locus_tag="ACZPAQ_00335"
     CDS             14552..15175
                     /locus_tag="ACZPAQ_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR0902449.1"
                     /translation="MKQRKDKGKYLAGWIRALLALILVGVLSFCGLFACVMYGAYDHI
                     QGEPEVMVILGCQVRQDGPSVLLRDRLDEALDYLEDHPDMTVVVSGGQGPDEPTSEAQ
                     AMADYLTAHGVEEEKVLLEPDSHNTAQNLDYSRRLLDREGIDTSDGVLLVSNGFHLTR
                     ARMLAERAGFEQVSTLAAPASHLPSRLYMYVREPLALVKSFLFDRAG"
     gene            15255..>15475
                     /locus_tag="ACZPAQ_00340"
     CDS             15255..>15475
                     /locus_tag="ACZPAQ_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR0902450.1"
                     /translation="MLEKLAAIEAKYSQIEARLAAPETYDDPAAVARLNKEQNELSPV
                     VETYRSYLAARERLAQAEELLSDPEMRELA"
BASE COUNT         2927 a         4669 c         5023 g         2856 t
ORIGIN      
        1 cccgggggga cggccaggtg ggggaggatg tgacggagaa cctgcggacc gtccggtcca
       61 tccctctgcg catcccggac gcccccgccc ggctcatcgt ccggggggag gtctatatgc
      121 ccaaaaaggt gttccacgcc ctgaacgagg agcgggagcg ccggggggag gccctgttcg
      181 ccaacccccg gaacgccgcc gccgggtccc tgcgccagct ggatccccgg gtggccgcct
      241 cccgccgcct ggacatcgcg gtgttcaacg tccagtgggc ggagggggag accttccgaa
      301 cccatgtgga gacgctggaa tatttgaagc agaagggctt taaagtgatc ccgcattaca
      361 gctgccgcgg cgtggaggat gccatcggac aggtcgccgc tatcggagag ggccgggagg
      421 acttcccctt tgacatcgac ggtgcggtca tcaaggtcaa cgacctggcc cagcgggagc
      481 tgctgggcag caccgcgaaa tttccccgct gggcggcggc ctataaatat cccccggagg
      541 taaagtcctc ccgggtgctg gacatcgtga tccaggtggg ccgcaccggg gtgctcaccc
      601 cccgggcggt gctggagccg gttcgcctgg ctgggactac cgttaccagc gccaccctcc
      661 acaaccagga ttttattgcg gagaaggacg tgcgcatcgg ggacaccgtc ctggtgcgca
      721 aggcggggga gatcatcccc gaggtgctct ccgtggagct tgagaagcgc ccggagggag
      781 ccgagcccta ccgcttcccc cagacctgcc cagtgtgcgg cgcgccggtg gagcgggacg
      841 aggacggggc ccacatccgc tgtaccgggg ccgagtgccc cgcccagctg ctgcgcaacc
      901 tggcccactt tgccagccgg gacgccatgg acatcgacgg gctgggcatc gcggtggtgg
      961 agaacctggt gaacgcgggt ctggtgaaaa cgccggggga cctgtacttc ctggaagccg
     1021 gcaaggtggc ggaactggac cgcatgggaa agcggtcggc ccagaagctg ctggaggcca
     1081 tcgagcgctc caagacccgg gacctgtccc ggctcctgtt tgcctttggc atccggcagg
     1141 tggggcagaa ggcgggcaag gtgctggctg cccggttccg caccctggat gcccttcagg
     1201 gcgccacgct ggaggagctg accggggtgg acgacatcgg ggccattacc gcccagagcc
     1261 ttctggactg gtttgccagc ccccagagcc agcacctcat tggccgtctg cgggaggccg
     1321 gtgtgaacat ggaggcggag gagcagggga gcgaccagcg ctttgccggc atgaccttcg
     1381 tcctcaccgg gtccctggag cggttcaccc gggacgaggc ctccaaaatg atcgaggacc
     1441 ggggggggaa ggtatcctcc tctgtgtcca aaaagacatc ctatgtggtg gcaggggcgg
     1501 ctgcgggctc caagctgcgc aaggcccagg atctgggggt gcctgtgctc accgaggagg
     1561 aatttctggc cctgatggac taactgatat ttgtcctggc aagaaagttg tgctatactg
     1621 tacccggaag cgcccgatgt gcggcggggg agagaggaga gcgggatctc atgaaaaagg
     1681 gctatctcta tatcattgtg accattgtga tgttcacctc ctatgaggta gtcctgcggt
     1741 atatcgcggg gcagatcaac tccatgcagc tgaccctgtg ccgcttctcc gtgggctttc
     1801 tgatcctgct gcccctggct ctgcggaccc tgaagcgccg gggaaagcgc ctggacggcc
     1861 ggagcatggc ccgtttcgcc ctgctgggcc tgctgggcat cgctctgagt atgcccatcc
     1921 tccacctgtc ggttcggtac gccgggtcct ctgtggccgc tgtgctgttc agctgcaacc
     1981 cggtatttgt cacctttttg gccttctttc tgctgaagga gcccatcaag ccccggcata
     2041 tctccgccct ggccctggag atcgcgggca ccgtggctat catcgaccca ttaggtcatg
     2101 cgctgaaccc cctcggggtg gggctggccc tgctgtccac cctgctgttc tccctgtacg
     2161 gcgtgctggg caagcggcgg gtggcagagt acggcggcgc ggtggtcacc tgcttcagct
     2221 tcctgttcgg cagcctgatc gttctggggc tgatcctgct ttcccatgtc ccggcggtgg
     2281 cccaggggat gacggccgcc ggtctggagg agttcgcccg catccccatc ctggcggggt
     2341 acaccctgga aaacctgccc tacgttctgt atgtgtccgt gggggtggcg gggatcggct
     2401 tctgctgcta cttcctcgcc atggagcagc tccccgccag cgtggtctcc ctggtctact
     2461 ttttcaagcc tgcgctggcc ccggtgctgg cctggcagct ccacggcgag gcgatccccg
     2521 ccaacatgat ggccgggatc ctgctcatcg tggcgggctc cctgtgcacc atcattcccg
     2581 ggcttctgga cgcgcgccgg aagcagcggg cgtaagggcg ggagaccgtt cccggaacgc
     2641 ggcccacatc cataccaaag aatgaggagt gaaacttatg tcagtttcca aggtatattt
     2701 tactgatttt cacacaaagg cgttcggtga tggactgcct accaagctga aaaagctgat
     2761 ccgggcggcc gggatccaga agctggatct ggacggcaag tttgtggcca tcaagatgca
     2821 cttcggcgag ctgggcaatg tgagctttct ccgccccaac tatgcccggg cggtggtgga
     2881 tgtggtgaag gagctgggcg gccgcccctt cctcaccgac tgcaacacca tgtaccccgg
     2941 cagccggaag aacgccctgg agcacctgga gtgcgcctgg cagaacggct ttactccgct
     3001 gacggtgggc tgccccatcc tcatcggcga cggcctgaag ggcaccgacg atgtggaggt
     3061 ccacgtggcg gggggcgaat atgtggagaa ggccaagatc ggccgggcgg tgatggacgc
     3121 ggatgtgttc atctccctga cccacttcaa gggccatgag atgaccggct ttggcgggac
     3181 catcaagaac atcggcatgg gctgcggctc ccgggcgggg aagaaagagc agcacgccag
     3241 cggaaagccc aagatcgacc cggaggcatg ccgcggctgc cggcgctgcc agaaggagtg
     3301 cgccaacaac ggcctggtct ttgacaccga gaccggaaag atgaccgtgg accaggacag
     3361 ctgtgtgggc tgcggccgct gcctgggggc gtgcaacttt gacgccatct acttcaccaa
     3421 cgacaacgcc caggcggccc tgaactgccg catggcggag tataccaagg cggtgctgga
     3481 cgggcggccc aacttccata tctccctggt ggtggacgtg tcccccaact gcgactgcca
     3541 cggcgagaac gacgtgccca tcctgcccaa tctggggatg ttcgcctcct tcgaccctct
     3601 ggccctggac caggcctgtg tggatgcctg cctggcggcg gagcccatgc cgggcagcca
     3661 gctggcccgc cacctggcgg agcccggctt ccacgaccac cacgaccact tcaccaactc
     3721 cacccccgag tcggagtggc gcagctgcct ggagcacgcg gccaagatcg gcctgggttc
     3781 ccgggagtat gagctgatcc gcctctgacc cggtcccgac aggggagggg cgcgccctcc
     3841 cggaaaaatc agacaagaaa aggacgatgc actatgccca ccaacacata cgaaagcccg
     3901 ctctcctccc gctatgccag cgatgagatg ctctacctct tttccgccga caagaagttc
     3961 tccacctggc ggcggctgtg ggtcgccctg gcccgggcgg agatggagct gggcctgccg
     4021 gtcacccagg agcaggtgga cgaactggag gcccacatca ccgacatcga ctatgagaag
     4081 gccgcccagt gggagaaaaa gctgcgccac gatgtgatgg cccatgtcca cacatacggg
     4141 gagctgtgcc ccaaggccat gcccatcatc cacctggggg ccaccagctg ctatgtgggg
     4201 gacaacaccg atgtgatcat catgcgggag gggctggagc tggtccggaa caagctggtg
     4261 caggtcctgt ccagattggc ggacttcgcc cgggaataca aggccctgcc caccctgggc
     4321 ttcacccact tccaggcggc ccagctggtc accgtgggca agcgggccac cctttggatg
     4381 aatgagctgc tgatggacct ggaggaggtg gagcaccgca tctccaccct gaagctgctg
     4441 ggttccaagg ggaccacggg gacccaggcc tccttcctgg agctgttcga gggcgaccat
     4501 gagaaggtga aggagctgga gcgccggatc gcggaggagc tgggcttcga cgccgtggtc
     4561 ccggtcagcg ggcagaccta ctccaggaaa atggactaca acgtgctgtc caccctggcg
     4621 gggatcgccc agagcgccag caagttcgcc accgacctgc ggcttctgtg ccacctgaag
     4681 gaggtggagg aaccctttga gaagaaccag atcggctcct ccgccatgcc ctataagcgc
     4741 aaccccatgc ggtgcgagcg gatctgctcc ctggcccgct atgtgatcgt ggacgccggg
     4801 aaccccgcta tcaccactgc cacccagtgg ttcgagcgca ccctggacga ctcggccaac
     4861 aagcgcatct ccatcccgga ggccttcctg gccgtggacg ccatcctgaa catctatatg
     4921 aatgtggcct ccggcctggt ggtccacccc aaggtcatcc gccgccatgt gatggaggag
     4981 ctgcccttca tggccagtga gaacatcatg atggacgcgg tgaagcgggg cggcgaccgc
     5041 caggagctcc acgagcgcat ccgggtcctg tcccaggagg cggggcggaa tgtgaaggac
     5101 ctgggcctgt ccaacaacct gatcgacctg atggcggagg acccggcctt cggcatgagc
     5161 cgggaggagc tcaccgccca tctggagccg gagcgctaca tcggccgctg cccggagcag
     5221 gtggaggagt tcctgtccca ggagatcgcc ccggtgctgg agaaatatgc tgcggctctg
     5281 ggtgggaagg aaacggagct gaaggtttga tggagaaagg cgggctgctt tgaagcatca
     5341 aaagtacggc gcctatctgg ccgccggggt caccgccttc gcggtgatcg ccgcagcgat
     5401 cctgctgttc tttgtgatct accacttttc ggcggtgcgg gccctgttgg agctgctggc
     5461 ctatatcctg cggcccatct tttatgggct ggtgctggcc tttctgctgc tgcccgtcca
     5521 ccggcagatc tactcctttc ttgccgcgct gtgtgaggga gtggcggagc gcgggggacg
     5581 gaagcgcgcc gcgctgaatt ttctggccat tgtgctcagt cttatttttg cggtactggt
     5641 ggtctatgtg cttctggcca tggtgctgcc ccagttgtac ctcagcatcg tggggctggt
     5701 gaacgcggtc ccggagtaca ttcggatcat gcagcagtgg ctgctggact ttttggcgga
     5761 caatccggag atccagacgg cggtgctgcc ctattacaac tccctggccc agtcggtaga
     5821 gcagtggctc agctccgaca tgctccccaa cctggagtcg gtgaacagca cactggactg
     5881 gctgaaggcc gagatcctgc cccagctcac cggcgtggtg agcagcgtct cctccggcgt
     5941 ggtggccctg gcgctgctgt tgaaggacat cctgattgcg attatcgtct cagtctatct
     6001 gctggcaagg aaggatgtct ttgccgctca atccaagaag atcgtgtaca gcgtgttccg
     6061 cactgatgtg gcggatctgc tggtggagga gacccggagc gcctaccgca tcctcagcgg
     6121 atttatcaac ggcaagctgg tggactccct aatcatcggc attatcgccc tggtgtgctg
     6181 caatctgttc cggttcccgt accctatgct gttggcagtg atcatcgggg tgaccaacat
     6241 catcccgttc ttcggcccct tcatcggggc catcccctgc gcgctgctga tcctgctggt
     6301 cagtcccctg aagtgcctgt attttgtcat cttcatcttt gtgctccagc agtttgacgg
     6361 caacatcctg ggccccaaga tcctggggga ctccacaggg ctggcctcct tctgggtgct
     6421 gttctccatc ctgctgttcg gcggcctctt cggctttgtg ggcatggtgc tgggtgttcc
     6481 ggtattcgcc atgttttaca gcgtggtcag ccgactggtg cgctgcggcc tgaaaaaacg
     6541 cgggctgccc ctggagaccg agcagtacct ggggcggacc ggaccgctgg ccggaacagg
     6601 tccaggggtg gaggactccg ggacccggtg agccggtcca tatcccttta tccgcccgga
     6661 gaggcggaca agttaaaaaa caaagctgcg gcccgtacgt tcaaacgcac gggccgcagt
     6721 tttttcattg cggttatgaa gtatcaagaa gagtgtctta caactgggtg gtcaggacct
     6781 tcttcagacg ggcgaagcgg ggcagaccgt tgacccgggg agccttggcg tcgccctgga
     6841 tcagggggag gacatagtcg atgaactgct ggttgacgcc gttgcccgcc tcattgatcc
     6901 actcgcgggg gaccttcttc tcgaagttgg ccacgatgtc caggggctcc agaatggtct
     6961 cacagcggta gccgtcctcg ccgcgggtgc agcggaaggc caccatctta tcggtaaagc
     7021 cctccacggc ggcctggacc gcggcggagc cggccagcag agcctcctcc gcgtcggtct
     7081 cagagtccag atgggcaccg cagcgctgga ggagggagag ctcgatgcca cggaccttca
     7141 caccgaaggc ctccttcacc gcggaagcca ggtgggaggc cagaccgccc agctgggcgt
     7201 ggccgaaacc atcggtggca gaggtcttgg cctcggagac aaaggtgccg tcggcgtagt
     7261 ggatgccctc ggacacggcc accaggcagt ttccgctttg gtcgtagacg gccttgacgt
     7321 cagcgaggaa gcgctccatg tcgaagtcgg tctcgggcag atagaccagg tcggggccgc
     7381 agccggtgag ggaggccagg gcggcggagc cggccagcca gccggcgtgg cggcccatga
     7441 tctcaatgat ggtgaccatg ccggtgtcat agacatgggc gtcctgccag acctcagcgc
     7501 aggatgtggc gatgtacttg gctgcagagg cgaagccagg gcagtggtcg gtgccgttca
     7561 ggtcattgtc gatggtcttg ggtacgccca tgatgcggca ctcatagccc accttctgca
     7621 tatacttgga gatcttgttg caggtgtcca tggagtcgtt gccgccgttg tagaagaagt
     7681 agcggacgtc gtactttttg aagatctcca gaatacgccg gtaatcggcg tcgtccacat
     7741 cggggtcggc cagcttatag cggcaggagc ccagggcaga ggagggagtg aacttcagca
     7801 gatccagttc cccggagtcc tcctgtccca tatcgtacag cttgtcctcc aacacgccct
     7861 tgattccgtg ggcggccccc agaacgtggg tgatgcagtc agccttcaaa gcggtctgaa
     7921 tcacgccctg agcactggcg ttaataactg cggtaggacc tccggactgg ccaatgatgc
     7981 aggcgccggt cagttgcttc atgtttgctt cctcctaaag tgtcaaattt ggatccaaaa
     8041 tcagcttagc ccaaaattct agcttaaatt gtagcacaca cccttgtatt tttcaatcgg
     8101 taatggtaca atatgaattg taaaaatatt tcaaggagat ggtcaatatg ccattggtta
     8161 ccacgactga aatgttcaag aaggcctatg acggcggtta cgccatcggc gccttcaatg
     8221 tcaacaacat ggagatcgtt cagggtatca ccgaggctgc tcaggaggag aaggctcccc
     8281 tgatcctgca ggtgtccaag ggcgcccgcg cctatgccaa ccacacctat ctggtgaagc
     8341 tggtggaggc cgctgtgatc gagtgccccg atatccccat cgcgctgcat ctggaccacg
     8401 gccctgattt tgagacctgt aaggcctgca tcgacggcgg cttcacctcc gtgatgatcg
     8461 acgccagttc caagcccttt gaggagaata ttgccatcac caagaaggtc gtcgagtacg
     8521 cccacgacca cggcgtggtg gtcgaggcgg agctgggcac cctggccggc gtggaggacg
     8581 atgtgaaggt ctccgccgag gattcctcct acacccgccc cgaggaggtg gaggagttcg
     8641 tgaccagaac cggctgcgac tccctggcca tcgccatcgg caccagccac ggcgcctata
     8701 agttcacccc tgcccagtgc acccgcaacg agaagggcat cctggtcccg cctcccctgc
     8761 gcttcgatgt gctggaggag gtctccaagc gcctgcccgg cttccccatc gtgctccacg
     8821 gctcctccag cgtgccacag gagtatgtga agatgatcaa tgagcacggc ggaaagatgc
     8881 ccgacgccat cggcatcccg gaggaggagc tgcgccacgc cgccgagctg tccgtgtgta
     8941 agatcaacat cgactccgat ctgcgcctga ccatgaccgg caccatccgc aagttctttg
     9001 aggagcaccc cgacaagttc gacccccgcg agtacctgaa gcccgcccgc gccaacatca
     9061 aggatctggt ccgacacaag ctgatccacg tcctgggctg cgccggcaag gcctgatccg
     9121 gtcccgcgcc gcagcatctg atataccatg aaaaaggagc gcttcggcgc tccttttcta
     9181 tggcattttt ttggaatctc tgatagaata ggttcataaa agaaagcatc agggagtgag
     9241 gcgcaaaatg gatcgtcatt tcttttttct ggcttacctc accgccaaca aacagcagag
     9301 gcgatggctg gtcataatat gggccgtcgt tccccttctt tttgctttgg caatgttctt
     9361 gaccctcagc ccatatcgcg gaatatcata tcaatatcag gagtcttatc caatcgttgg
     9421 gaccgaaaac cggcagctgt ggaccttgaa gggttctgag ttggttaccc tgtttaatga
     9481 aaatctccct gacacagcgc ctgaactctc ctatttacac gaaccaacgc cgtccgaccg
     9541 tcagatcatg ctgacggata atggtaagac gtggtctatt gtatttagac aggttccgga
     9601 agacgcagcc agcatctatt tctggtccaa acagccggaa atagatgcgt ggcttggcaa
     9661 tgtagaggat gtgaagctct cgctgtatca cacctccgcc caggacattc tcttaaatga
     9721 gcagtatgct cgatgtctca tcaacatctt cacccctggt gcggaggact atgttgtccg
     9781 ccgccttcac ctttcccgtc cccttacgtc cggctacaaa cgagttaaga ccggcgatgt
     9841 tctttataca cataaaggtg gaacctctcc tgtacttatc attgagcctg attgcagaaa
     9901 ttggcctcca gaccgttgat cttcgccccc agccgggggc ggcttttttg tcctgtttcg
     9961 ttccgcctct tgaagccctc cggcggccgg tgcggaagaa ttccgcaaaa ttggacgctc
    10021 cggcttgtct attgccgggg attgtgataa aatacatggt cagcgtgtaa actgtaaaca
    10081 tgatccagat ccttgataaa ggtggtttga taacatgaag atcgtagtat tggccggtgg
    10141 actcagcccg gagcggaacg tctccctgtc ctccggcgcc atggtgtgcg aggcgctgcg
    10201 ttccaagggg caccaggtgg ccctggtgga cctgttcttc ggcatggagg ggaccgatca
    10261 gacggtggac cccttccagg cccccatccc ccaggcgttc aaaaaggtga gtcctcaggc
    10321 ccccgacctg gagcaggtgc gggccagccg cagcgaccgg agcccttctc tcatcggtcc
    10381 cggggtgctg gaactgtgcg ccggggcgga tgtggtctat ctggctctcc acggggcctg
    10441 cggagaggac ggacgcatcc aggccgctct ggacctgctg ggggtgccct acaccggctc
    10501 caactgcctc agctccgcca tcgctatgga caaggatatg actaagcgcc tggtggccga
    10561 tcaggtgcgc accccccgct ggcagacggt gaccgtcaca gcggacaatc tggacgtcct
    10621 gacggaggag accgcccttc ccgcggtggt aaagcccatt gccagcggct cctccatcgg
    10681 cgtgaccatc gcccacaccc gggaggagct gcgccggggc ctggaggaga gcgtccgttt
    10741 gggcggacgc acggtcctgg agcagtacat caaggggcgt gagatccagg tggcagtgct
    10801 caacggccgg gcgctgccct ccattgagat catcccccag gcggacttct atgactacgc
    10861 caacaagtac cagcccggcg ccgcccggga ggtgtgtccc gcagagattc caccggagtg
    10921 ggagaaggca gtgggagagg cggccctggc cgtgttccgc accatcggcc tgtccgtcta
    10981 tgcccgggcc gacttcatcg tgaccgagga cgggaccccc tatttccttg aaatcaacac
    11041 gctgcccggc atgactccta ccagcctggt gccccaggag gcggcggcgg ttggtatcgg
    11101 ctatgccgac ctgtgccaga ccatcgtgga ggcctctctg gaggcccggc gggaaggagt
    11161 ttaagggcag atggaagcca tcacagtacg ggacctgctc accgccgtgg gcggtacgct
    11221 gatcgggccg gaaaccagtc tggaccggac ggtcagcagc gtggatacag acagccggga
    11281 catccacccc ggttccctgt tcctccccct ggtgggggag cgcttcgacg gccacaccta
    11341 tatcggctcc gccctggagg cgggagccgc cggatgcctc actgcccggg agccggagca
    11401 ctatcaggag ggacgcttct acatcaaggt agaggacacc cagcgcgccc tgcgggacct
    11461 ggccttctgg tacaaggagc aattccggat ccccttcgtg ggggtgacgg gcagtgtggg
    11521 caagaccaca gccaaggaca tgatcgcctc ggtcctgtcc gtgaagtaca aggtcctcaa
    11581 gacggaaggg aacttcaaca acaacgtggg cctgcccctg accctgctcc ggctggaccg
    11641 cagcgttcag atcggcgtgg tggagatggg gatggaccgg tttggggaga tcgattacct
    11701 gggtgacatc gtccgcccgg aggtgggggt catcaccaac atcggcgacg cccacatcga
    11761 gcggctgggc agccgggaga acatcttccg ggccaagtgt gagctgctgc cccacatccg
    11821 caaggaaaac ggcctgctga tcctcaacgg ggacgacgcc ctcctgtcca cccttcgggg
    11881 ccggaccccg gtgcccgcgg tgttctgcgg cagcggggag ggcctggagt accgggccca
    11941 gtcggccagc agcgaggggc tggactccct gcgcagccgt ctcaccacgc cggccatgga
    12001 ccgggaggtg tgcatccccg ccctgggcag ccacatgatc tatcccaccc tcatcgccgc
    12061 ggcggtgggg gagcacttcg gcctgacagc cgatgagatc gagcagggga tcgcccgctt
    12121 cgtccccacc cggatgcgga tgaatctggt gcagcggggg aacgggatca ccattctgga
    12181 cgacacctac aacgccaatc cccagtccat gcgggcggcc atcagtgtgc tggcggacag
    12241 ccgcagcgcc tggaagctgg cggtcttggg ggatatgttc gagctggggc cttactcgcc
    12301 cgccctccac accggagtgg gggagtacct gggcaagcgg aagatcgact gcctggttgc
    12361 ggtgggcgat caggcccgct acctggcgga gggggcacag tctgccggca tgaccgccgt
    12421 ctatccctgc gcggacaaga aggccgcccg ggagatcctc ccagggctga tccggccgga
    12481 cagcaccatc ctggtcaagg catcccgggg gatgcggatg gaggagctgg tggaccagct
    12541 tctggagctg acgccggagc aataagaaaa aactgccgcc ccttcagtca gggaggggca
    12601 catcaatcaa accaaagagg acgctatgat cgacatatct gtctccaacc tggtgaaaga
    12661 gtttgaggtt ggcacgaaaa ttttagatgg gctcaccttc caggtggaca ctggggagcg
    12721 ggtgggcctg ctgggtccca acggctgtgg caagaccacg ctgctgcgca tcctcaccgg
    12781 cgcgatggac tatgacgagg gcgaggtgat gatcgccccg ggaaagcgga tgggcttgat
    12841 ctcccagatc ccggtctacc cggagggcta taccgtggag gatgtgctgg ccaccgcctt
    12901 tgagcccctg cgcaaaatgg aggacgagct ggcccggctc acccgggaga tggcctctgg
    12961 cacggacccg gccctgatgt cccggtatga caagctcacg acggcctatg aggcggccgg
    13021 aggctatgag acggacacca ggattaacaa agtgtgcaac ggactgtcca tcccacaggc
    13081 catgcgggag cagctgtttg aaagcctgtc cggcggggaa aagacccggg tgaacctggc
    13141 ccgtctgatc ctggaggaca ccgacatcct gctgctggat gagccgacga accacctgga
    13201 cctgcgggcc accgagtggc tggaggagta tctgggcaag ttcaagggga cggtgctcac
    13261 cgtctcccac gaccgttatt ttctggacca ggtggtggac cgcatcatcg agatccagga
    13321 gggccgggcg gaattttacc agggcagtta cagcttctac gccgtggaga aggagcgccg
    13381 ctatgaggag aagctgaagc agtacgagaa ggagcaggcc aagatcgagc agctggagaa
    13441 ggccgcagag cagatgcggg tgtgggccta ttccggaatg gacaagacct ttaagcgggt
    13501 caaatccatg gagaagcgca tcgagcggat gcgggtcacc gaccgcccca aaaaggagcg
    13561 gaagatggag gtccgcttcg gagagcggga gttccgcggg gacgaggtgc tgaccatcaa
    13621 gggcctcacc aagggcttcg gcgggcgcac cctcttttcc ggcctggggc tggaagtggc
    13681 cggcggcgag cgcatcgccc tgatcggaga caacggcagc ggcaagacca cgctgctgaa
    13741 aatgatcctg ggcgaggagg cgccagacgg cggtaaggtc cggatggggc cgactgtaaa
    13801 ggttggatac ctgccgcagc acgtccactt tgaccacccg gagcgcagcc tggtggacac
    13861 cctgatctac gcccaggact gcactgccca gtcggcgcgg aaccggctgg cggcgttcaa
    13921 attccggggc gaggatgtgt tcaagccggt atccgccctg tccggcggcg agcagagccg
    13981 tctgcgcctg tgtatgctga tggacgaaaa gatcaatctg ctggtgctgg acgagcccac
    14041 caaccacctg gacattcaga gccgggagtg gatcgaggag gcggtggagg aatacgaggg
    14101 gaatctgctc ttcgtctccc atgaccggta ctttattgac cgctttgcca gccggatctg
    14161 gctgctggaa aatggacaga tcacggattt ctgcgggacc tatcaggagt tccaggccgc
    14221 ccgggcccgg ggcgcggcgg ggcaggcggc ctcagacacc gtccccgcgc cagcaaaggc
    14281 ggcggagccg gagcggaaaa aagagaagcc caagcggcct ggcgggacca agaacctgga
    14341 aaaagaggtg accgccgccg agcgggcggt ggccaaggcg gaggaggcca tggacgccct
    14401 ctccctccag atcgaggagg cctccgcgga ctatctgaag cttcaggagc tgtacgagca
    14461 gcgggaggct ctggaggcgg agctccaggg gctctatgac cgctgggaga ccttgtccgc
    14521 ccagctggaa gaggcgaggg ggtaaaagca attgaagcag agaaaggata aaggaaaata
    14581 ccttgccggg tggatccggg ctctgctggc tttgatcctg gtgggagttc tgtccttctg
    14641 cggcctgttc gcctgtgtga tgtacggcgc atacgaccac atccaggggg agccggaggt
    14701 catggtgatc ctgggctgtc aggtgcgtca ggacgggccc tcagtgctgc tgcgggaccg
    14761 gctggatgag gccctggact atctggagga ccacccggat atgacggtgg tggtctccgg
    14821 cggccagggg ccggacgagc ccaccagcga ggcccaggcc atggccgact atctcaccgc
    14881 ccacggagtg gaggaggaga aggtgctcct ggagccggac tcccacaaca ccgcccaaaa
    14941 tttagactat tccagacggc tgctggaccg ggaggggatc gacacctcgg acggagtgct
    15001 gttggtgtcc aacggctttc acctgacccg ggccaggatg ttggcggagc gggcgggctt
    15061 tgagcaggtc tccacgttgg cggcccccgc cagccatttg ccctcccggc tgtatatgta
    15121 tgtccgggag ccgctggccc tggtgaagtc cttcctgttc gaccgggcag gttaggcgct
    15181 aagagttagg aattaggaat gaggaatttg gcggcctgcg gccgcccgtt gaagatccaa
    15241 tgctggaggt tcctatgctg gagaaacttg ccgccattga ggcaaaatac agccagatcg
    15301 aggcacgact ggccgccccg gagacctatg acgaccccgc cgcggtggcc cgtctgaata
    15361 aggagcagaa tgaactgagc ccggtggtgg aaacctatcg aagctatctg gccgccaggg
    15421 agcgcctggc tcaggcagag gagctgctgt ccgacccgga gatgagagag ctggc
//
LOCUS       DBMDSJ010000005        13083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_1682_length_13083_cov_2.231628, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000005 DBMDSJ010000000
VERSION     DBMDSJ010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 13083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13083
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1682_length_13083_cov_2.231628"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="ACZPAQ_00345"
     CDS             <1..178
                     /locus_tag="ACZPAQ_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR0902451.1"
                     /translation="GDILNIAPQTAPLFMGIGMHCLGCPASRGETVEQACMVHGVDVD
                     DLLQQVNAMIAAEN"
     gene            251..847
                     /locus_tag="ACZPAQ_00350"
     CDS             251..847
                     /locus_tag="ACZPAQ_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR0902452.1"
                     /translation="MKYTVLMGSPRKNGNTAALLRAFMEENRDMGIEQDMIWLYDRTI
                     LPCLSCKQCQSTPWGTFGCPQEDDMQAIFDSVQTSEVLILASPIYSWYCTPPMKAVMD
                     RLIYAPNKYYGPEGRQPALWKLKQVAAIASCGYHPDRGADLWDEGLKRWCKHGEMDYL
                     GMLCRRDFGPQEPFMNEERDEAARDFAHALYLTVSLSL"
     gene            850..1566
                     /locus_tag="ACZPAQ_00355"
     CDS             850..1566
                     /locus_tag="ACZPAQ_00355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0902453.1"
                     /translation="MEKRAELSPRLQGVAEMIPEGARLADVGTDHAYLPAWLLQNGRI
                     PSAIASDVREGPLSRARATARACGCFERMSFRLCDGLSGIAPEEVDALVIAGMGGETI
                     ANILEAAPWVKEKDFPIILQPMSTQAELRGWLWRNGFLCLREKTVFEGETLYSIILAR
                     QGGAVPMTPAEEWAGRQYPGMEDPLRGLLLDHLLGRVRRALDGMARSRAPAEARRREL
                     EQVLEGLMEMRKEWDAWQQR"
     gene            1551..2351
                     /locus_tag="ACZPAQ_00360"
     CDS             1551..2351
                     /locus_tag="ACZPAQ_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR0902454.1"
                     /translation="MAAAIIDVYRALDQWAPFDTQMDFDNAGFLVGRGGREVGKILVA
                     LDITEEVAEEAERWGTQLIVAHHPVIFHPARQITDGTPTGRILLRLIEGGVAAICAHT
                     NLDAAQDGVNECLARALGLTELEQLHQDGVDRNGRPYGVGRVGRCPQAGCSGTEYARL
                     VKEALGAAGVRLTDGGRPVSRVAVGGGSCGSMLGDAAALGCDTFVTADVKYDQFLEAK
                     ALGITLMDAGHYATEQVVCPKIVEYLARCFPALEVRMSRTHREVYGWV"
     gene            2543..2884
                     /gene="rplS"
                     /locus_tag="ACZPAQ_00365"
     CDS             2543..2884
                     /gene="rplS"
                     /locus_tag="ACZPAQ_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR0902455.1"
                     /translation="MDLMQSFTQKHLKAEPPVVTVGDTVRVHLKVKEGNRERIQIFEG
                     TVIAKKHGVIEETFTVRRISYGIGVEKVFPLHAPSIEKVEVVRHGKVRRAKLYYLRDR
                     VGKAAKVKEAL"
     gene            2968..3846
                     /gene="ylqF"
                     /locus_tag="ACZPAQ_00370"
     CDS             2968..3846
                     /gene="ylqF"
                     /locus_tag="ACZPAQ_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR0902456.1"
                     /translation="MNIQWYPGHMTKTRRQIEADLKHVDIVVEIIDARIPVSSRNPDI
                     DAITAGKPRLVVLNRADQADPAGNRAWGAWFRKKGWSVLETDAKTGAGVSQFSRVVQE
                     VLKDQIARWQEKGLVGRPVRAMVVGVPNVGKSTFINKVARKKSAKASDRPGVTRGKQW
                     VTVDRGLELLDTPGILWPKFEDVETGMRLAFTGAVKDDIMDVETLGCHLMAFLGTQYP
                     NVLAESYRLKEVLAREAEENEVAYGYRLLEACAQKRGMRISGGEFDTERMARVLLDEY
                     RGGKLGRFTLELPEEV"
     gene            3880..4488
                     /locus_tag="ACZPAQ_00375"
     CDS             3880..4488
                     /locus_tag="ACZPAQ_00375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR0902457.1"
                     /translation="MDELWQYEREALAVGHTAVCGCDEAGAGPLAGPVYAAAVILAPG
                     TELPGLNDSKKLTEKKREALFPLIQEKALSWAVAWVDAEEIDRTDILSARMKAMQLAI
                     DRLNPTADFALIDGNRDRGRSAAITTEHRTIVKGDSLSASIAAASILAKVSRDHWMVR
                     AAEEYPQYQFDRHKGYGTKLHYELLRQYGPSPIHRRSFLKNL"
     gene            4485..4853
                     /locus_tag="ACZPAQ_00380"
     CDS             4485..4853
                     /locus_tag="ACZPAQ_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR0902458.1"
                     /translation="MSGEDSRLRGRWGEALAAEFLRRKGYRIIASGWRCRFGELDLVA
                     EGGEFLCFIEVKLRGSERFGTGAEQVDRRKQERLRTTAELYLQQHPSPLQPRFDVVEI
                     LAPQGMATKRPGIRQIENAF"
     gene            5003..6487
                     /gene="thrC"
                     /locus_tag="ACZPAQ_00385"
     CDS             5003..6487
                     /gene="thrC"
                     /locus_tag="ACZPAQ_00385"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR0902459.1"
                     /translation="MQYISTRDREQQFSASQAIAKGLAEDGGLLTPVYLPKLPRRALE
                     ELKNMSYQQRAVYVMKLFLDEFSVKELTDFANAAYGPEKFDTPAVAPVRALDGNTYCL
                     ELWHGPTCAFKDMALQMLPHLLTASLRKQEEEKTVCILVATSGDTGKGALEGFRDVDH
                     TKILVFYPKDGVSAIQELQMVTQEGANVGVCSVVGNFDDAQTGVKRLFSDEKLREQLA
                     RRGYFLSSANSINWGRVLPQIVYYISAYCDLLRDGRLELGDPVNVCVPTGNFGNILAA
                     YYARDMGVPIQKLICASNSNNVLTDFLSTGTYDRNRTFFNTISPSMDILISSNLERLL
                     LAITQEDSEVRGYMEQLNATGRYQVSDRVREKIQKLFKGYCCGDEETKRVIAAIHEKF
                     GYLIDPHTAVGFSALSQYRAETGDETAAIVASTASPFKFCTDVLDALGVSDRAQGLDV
                     LDQLSQVTGEPVPAPLAALRSKESRFRQTVEKEHMVDAVLGMLE"
     gene            6497..7183
                     /locus_tag="ACZPAQ_00390"
     CDS             6497..7183
                     /locus_tag="ACZPAQ_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR0902460.1"
                     /translation="MALYAIGDTHLSLGADKPMDVFGGGWSGYVDKLRAGFAEIGPED
                     TVVLCGDLSWGMSLEQAREDFAFLDALPGRKILLKGNHDYWWTTAAKMERFFAENGFS
                     TLEILHNNCRLYGGVALCGTRGWFYEEDREGHGAKIFNRELIRLEASLKAAGEREKYC
                     FLHYPPLYQGYRCQEIIDLLERYQVSRCFYGHLHGGSHRLAISGRQGGVEYDLVSADY
                     LGFQPKKILD"
     gene            7334..8767
                     /gene="tig"
                     /locus_tag="ACZPAQ_00395"
     CDS             7334..8767
                     /gene="tig"
                     /locus_tag="ACZPAQ_00395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR0902461.1"
                     /translation="MKVTNVEKKENSTVELTIHVEADEFEAAVQKAYLKSRSRIAVPG
                     FRKGKAPRKIIEGMYGSGVFYEDAINDVYPGAYEAAVKEQGLEDMGHPVMDIVEVNKE
                     KGLIFTALVSVRPEAKVKAYKGLTAPKAEVSVTDEDVENELQPYVRRATRLISVDREV
                     KLGDTAVIDFEGFEDGKPFEGGKGENYSLEIGSGSFVPGFEDQIIGLKAGDEKDLDIT
                     FPEDYVADLAGKAVVFKVKVKEVKESQLPTVDDEFAKDVSEFETLADFKKDLGEKLTQ
                     RRTAEAQNDFEQAILEQLVDNLEADIPNGMVETQLDKIMDEYAMRMSSQGMSMDDYLK
                     MMGMTPEMMRASSKPAALNQVKTELALEAVAAAENLEITDEECEAEVNKLAEQYKLTA
                     EQVKAAVSLDALKHDLRLQKASELVVAEAKVGEAPKKEEEAAEKPKKATRKKKTEEAA
                     EESESAEKPKRTRKKKAEESAEEPKAE"
     gene            8917..9498
                     /gene="clpP"
                     /locus_tag="ACZPAQ_00400"
     CDS             8917..9498
                     /gene="clpP"
                     /locus_tag="ACZPAQ_00400"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR0902462.1"
                     /translation="MSLVPYVVEQTTRGERSYDIFSRLLNDRIIFLSEEVNDTTASLV
                     VAQLLYLESQDPDKEIQFYINSPGGSVTAGMAIYDTMQYVKCDVSTICIGLAASMGAF
                     LLSAGTKGKRLALPNAEIMIHQPSAGTQGQITDMALHLKRLEVVKRRMNRILAANTGR
                     SVEEVTADCERDNFMTAEEALQYGLIDKVIEKR"
     gene            9568..10884
                     /gene="clpX"
                     /locus_tag="ACZPAQ_00405"
     CDS             9568..10884
                     /gene="clpX"
                     /locus_tag="ACZPAQ_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR0902463.1"
                     /translation="MAKNDDKQRAVRCSFCGKHQDQVRRLIAGPGAYICNECVQLCMS
                     ILEDERDTAQTAAAEVPDAIPTPREIHKVLDQYIIGQEQAKVALSVAVYNHYKRIYFG
                     GGDDVELQKSNILLMGPTGCGKTLFAQTLARVLKVPFAIADATTLTEAGYVGDDVENI
                     LLRLVQAADYDVELAERGIIYIDEMDKIARKSENTSITRDVSGEGVQQALLKILEGTV
                     ANVPPQGGRKHPHQEFIQIDTKNILFICGGAFEGIDKIIESRLDKRSMGFGAEIRSKK
                     ERDVGQLLKEIQPQDLLKFGIIPELVGRLPVITTLRSLGREQMVQILTEPKNSLVKQY
                     RKLMEYDDVDLEFTPEALEAVAGRAVERGIGARGLRAILEEVMTRIMYDVPSDPEVVK
                     VTITEDCIRSGAAPEILRDPEHSQRPALGQADLRAERGGLPGTNAG"
     gene            11051..>13083
                     /gene="lon"
                     /locus_tag="ACZPAQ_00410"
     CDS             11051..>13083
                     /gene="lon"
                     /locus_tag="ACZPAQ_00410"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR0902464.1"
                     /translation="MKKEWTMESASVMPVIALRGLTVFPNVLIHFDVAREISIKALEE
                     AMSSGSPVFLVGQKDISEERPEEKDLYTVGTISNVRQILRTPGDNVRVMVEGVSRGSL
                     ERLTRTGPYLEAEVQPIPSEQHERNSAKAEALIRSTYELFQQYTELAPKTAPDLLINV
                     LASEDPGYIADFIAQNIAMRNSDKQAVLEELRPVRRLERLHRLLGREVEILGLDMEIQ
                     NRAREQMADNQRDYYLREQMKAIQNELGEGEGGDSEIGDYRRKIAEAKLPDEVREKLE
                     KEVGRLAKQPFGSSEATVLRSYLDVCLELPWGKKTRERVSVEAARRTLDKDHFGLEKV
                     KERILEFLAVKQLAPDLKGQVICLVGPPGVGKTSLAMSVARALNRKLARLSLGGVSDE
                     AEIRGHRKTYVGAMPGRIISAINQAGSCNPLLLLDEIDKLGHDHRGDPASALLEVLDA
                     EQNSTFRDNFLELPFDLSDVMFITTANTTDTIPRPLLDRMEVIELPSYTDEEKLQIAK
                     RYLLPKEMKRHGLEKAQLKVSDGAIREMVASYTRESGVRVLERKLAGICRKAAMHLVS
                     EDVKQIRITEKELKDYLGAPRYYPERQALEERVGVVNGLAWTSVGGELLEVEVNVVPG
                     TGKVELTGNLGDVMKESAHAALSFIRSQADRLGIQGDFYQTKDIHVHFPEGAVP"
BASE COUNT         2596 a         3794 c         4467 g         2226 t
ORIGIN      
        1 cggtgatatc ctcaacattg cgcctcagac cgcccccctc tttatgggga tcggcatgca
       61 ctgcctgggc tgccctgcct cccgcgggga gaccgtggag caggcctgca tggtccacgg
      121 ggtggacgtg gatgatctgc tccagcaggt caacgccatg atcgccgccg aaaactgagc
      181 tgctgaaaaa gcggggcgga gcaatccgcc ccgctttttt attcattaaa caacagggga
      241 ggaaacagcg ttgaaatata cggttttgat ggggagcccc cggaagaacg gcaataccgc
      301 cgctctgctg cgggcattta tggaggagaa ccgggacatg gggatcgagc aggatatgat
      361 ctggctctat gaccggacca ttttgccctg cctgtcctgc aagcagtgcc agagcacgcc
      421 ctgggggacc ttcggctgtc cccaggagga cgacatgcag gccatctttg attccgtcca
      481 gaccagtgaa gtacttatcc ttgcaagccc tatctattcc tggtactgca ctccgcctat
      541 gaaggcggtg atggaccggc tgatctatgc acccaacaag tattatgggc cagagggccg
      601 tcagcccgcc ctttggaagc tgaagcaggt ggctgctatc gccagctgcg gctatcaccc
      661 cgaccggggc gcggacctgt gggacgaggg gctgaagcgc tggtgcaagc acggggagat
      721 ggactatctc ggcatgctgt gccgccggga cttcgggccc caggagccct ttatgaatga
      781 agagcgggat gaggcagccc gggactttgc ccacgccctt tacctgacgg tcagcctgtc
      841 cctgtgacta tggagaagcg ggcggagctg agcccccggc tccagggggt ggcggagatg
      901 atcccggagg gggcccggct ggctgatgtg gggacggatc acgcctatct tccggcatgg
      961 ctgctccaga acggacgcat ccccagcgcc atcgcctcag atgtgcggga ggggcccctc
     1021 tccagggccc gggctaccgc ccgggcctgc ggctgctttg agcggatgtc cttccgcctg
     1081 tgcgacggcc tctctgggat cgctccggag gaggtggacg ctctggtcat cgccgggatg
     1141 ggtggggaga ctatcgctaa tatcttggaa gcggcgccat gggtaaagga aaaagacttt
     1201 ccaattattc tccagcccat gagcactcag gcggagctgc ggggctggct gtggcgcaat
     1261 ggatttctat gcctacggga gaaaacagtt ttcgaagggg aaacccttta cagcataatt
     1321 ttggcgagac agggaggcgc ggtccccatg acgcccgccg aggagtgggc ggggcgccag
     1381 tacccgggga tggaggaccc gctccggggc ctgctgctgg accacttgct ggggcgggtg
     1441 aggcgcgccc tggatgggat ggcccgctcc cgggcgccgg cggaggcgcg ccgcagggag
     1501 ctggagcagg tcctggaggg cctgatggag atgcggaagg agtgggatgc atggcagcag
     1561 cgataataga cgtctaccgg gctctggacc agtgggcccc cttcgacacc cagatggact
     1621 ttgacaatgc ggggttcctg gtgggccggg gcggccggga ggtcggaaaa atcttggtgg
     1681 cgctggacat cacagaggag gtggcggagg aggcggagcg ctggggcact cagctgatcg
     1741 tcgcccacca cccggtgatc ttccacccgg ctcgccagat caccgacggg acccccaccg
     1801 ggcggatcct gctgcggctg atcgagggcg gcgtggcggc gatctgcgcc cacaccaatc
     1861 tggacgcggc gcaggacggc gtcaacgagt gtctggcccg ggccctgggg ctgacagagc
     1921 tggagcagct ccaccaggac ggggtggacc ggaacgggcg gccctacgga gtgggccgtg
     1981 tgggccgctg tccccaggca gggtgctccg ggaccgagta tgcccggctg gtgaaggagg
     2041 ccctgggcgc ggcgggggtg cgcttgactg acggaggccg tccggtgtcc cgggtagcgg
     2101 tgggaggcgg ctcctgcggc tctatgctgg gggacgccgc cgccctgggg tgcgatacct
     2161 ttgtcaccgc cgacgtgaaa tatgaccagt tcctggaggc gaaggccctg gggatcaccc
     2221 tgatggacgc gggacactac gccactgagc aggtggtgtg cccgaaaatc gtggagtatc
     2281 tggccaggtg tttcccggca ctggaggtcc gtatgtcccg gacgcaccgg gaggtctatg
     2341 gctgggtctg aggctgcctg cgtggtttct ggggatggac gcggtatcgg agagaaaatg
     2401 agagaaaaat gcttgcattt tgatccggaa tgtgataaaa tcccttagtc tgaaataacg
     2461 ggcggtcctc tgcggccgcc gcgcacaggg gtgcgcggca cgatggcgcg caagaacgcc
     2521 tctacaacag gaggaaaatg ttatggattt gatgcagtct tttactcaga agcacctgaa
     2581 ggccgagccc cccgtggtca ccgtgggtga caccgtccgg gtccacctga aggtcaagga
     2641 aggcaaccgc gagcgcatcc agatcttcga gggcaccgtc atcgccaaga agcacggcgt
     2701 catcgaggag accttcaccg tccgccgtat ctcctacggc atcggtgtgg agaaggtgtt
     2761 ccccctccac gctccctcca tcgagaaggt cgaagtggtg cgtcacggca aggtccgccg
     2821 cgccaagctg tactacctgc gcgaccgcgt gggcaaggcg gccaaggtca aggaggccct
     2881 gtaatccaac acgagaaagg agcggttttg ccgctccttt tttcgtacag tccccgcgaa
     2941 gcgggggaga ggaatggagg agaacccatg aatatccagt ggtatccggg gcacatgacc
     3001 aagacccggc ggcagatcga ggcggatctg aaacatgtag acatcgtggt ggagatcatt
     3061 gacgcccgga tcccggtgtc cagccggaac ccggacattg acgccatcac ggcgggcaag
     3121 ccccggctgg tggtgctcaa ccgggcggac caggctgacc cggccggaaa ccgggcctgg
     3181 ggggcgtggt tccggaagaa gggctggtct gtcctggaga ccgacgcgaa aacaggcgcc
     3241 ggggtgagtc agttctcccg cgtggtgcag gaggtgctga aggatcagat cgcccgctgg
     3301 caggagaagg gcctggtggg ccgtccggtg cgggccatgg tggtgggggt gcccaatgtg
     3361 ggcaagtcca cctttatcaa caaggtggcc cggaagaagt cggccaaggc cagcgaccgc
     3421 cccggcgtca cccggggaaa gcagtgggtg acggtagacc gggggctgga actgctggac
     3481 acgccgggga tcctgtggcc caagtttgag gacgtggaga ccgggatgcg cctggccttc
     3541 accggcgcag tgaaggacga catcatggat gtggagaccc tggggtgcca cctgatggca
     3601 tttttgggga cgcagtatcc caatgtgctg gcggagagct accgcctgaa ggaggtcctt
     3661 gcccgggagg cggaggagaa cgaggtggct tacggctacc gcctgctgga ggcctgcgcc
     3721 cagaagcggg ggatgcggat ctccggggga gagtttgaca ctgagcggat ggcccgggtg
     3781 ctgctggatg agtaccgggg tggcaagctg ggacgcttta ccctggaact cccggaagag
     3841 gtttaaggaa atagtatgag acaggagggc gggaaggcga tggacgagct ttggcaatat
     3901 gagcgggagg ccctggcggt cggtcacacc gcggtctgcg gctgtgacga ggcgggggcg
     3961 gggcccctgg ctggcccggt gtacgcggcg gcggtgatcc tcgccccggg gaccgagctg
     4021 ccggggctga acgactccaa gaagctgacg gaaaaaaagc gggaggcgct gttcccactg
     4081 atccaggaga aggccctctc ctgggcggtg gcctgggtgg acgcggagga gatcgaccgc
     4141 accgacatcc tcagcgcccg gatgaaggcc atgcagctgg ccatcgaccg cctgaaccct
     4201 acagcggatt tcgccctcat cgacggcaac cgggaccggg ggcgcagtgc cgccattacc
     4261 actgagcaca gaactattgt aaagggagat agcctgtcgg catctattgc ggcagcctcc
     4321 atcttggcca aggtaagccg ggatcactgg atggtccggg cggcggagga gtatccccag
     4381 taccaatttg accggcacaa gggctatggc accaagctcc actatgagct gttgcggcag
     4441 tacgggccca gccccatcca ccgccggtcc ttcctgaaaa acctgtgagc ggggaggaca
     4501 gccgcctccg gggccgctgg ggggaggcgc tggcggcgga atttctgcgg cggaagggct
     4561 atcggatcat agcctccggc tggcggtgcc gcttcggaga gctggacctg gttgcggagg
     4621 ggggggagtt cctctgcttt attgaggtga agctgcgggg gagcgagcgc ttcggcaccg
     4681 gggcggagca agtggaccgc cgcaagcagg agcggctgcg gaccacggcg gagctctacc
     4741 tccagcagca cccttcgccg ctccagcccc gcttcgacgt agtggagatt ctggcccccc
     4801 agggaatggc gacgaaaagg cccgggatcc gacagatcga gaacgccttt taaggaacgg
     4861 cgcgaaagat ctgtgcggat ttgcggcgtc ggggttgacc tcaaaatcaa aagatggcat
     4921 aatagtccat cagagggtcc gcgcgtgagc cggaccgtac caacccgtca acaggggaaa
     4981 ctaaagattg gaagagatcg agatgcaata catcagtaca agggacaggg agcagcagtt
     5041 ctccgcctcc caggccatcg ccaagggcct ggcggaggat ggcggcctgc tgaccccggt
     5101 atatctgccc aagctgcccc gccgcgccct ggaggagctg aagaatatgt cctatcagca
     5161 gcgggcggtc tatgtgatga agctgtttct ggatgaattt tccgtgaagg agctgacaga
     5221 cttcgccaac gcggcctacg gcccggagaa gtttgacacg ccggcggtgg ccccggtgcg
     5281 ggccctggac ggcaatacct actgtctgga gctgtggcac ggccccacct gtgccttcaa
     5341 ggacatggcc cttcagatgc tgccccatct gctcaccgcc tccctgcgca agcaggagga
     5401 ggaaaagacg gtgtgcatcc tggtggccac ctccggcgac accggaaaag gcgccctgga
     5461 gggcttccgg gatgtggacc acaccaagat cctggtgttc tatcccaagg acggcgtctc
     5521 cgccattcag gagctccaga tggtcaccca ggagggggcc aatgtggggg tctgctctgt
     5581 ggtgggcaac tttgacgacg cccagaccgg cgtgaagcgc ctcttctccg atgagaagct
     5641 gcgggagcag ctggcccggc gggggtactt cctgtcctcg gccaactcca tcaactgggg
     5701 ccgggtgttg ccccagatcg tctactatat ctccgcctac tgcgacctgc tgcgggacgg
     5761 caggctggag ctgggcgacc cggtgaacgt gtgtgtgccc acgggcaatt tcggcaacat
     5821 cctggcggcc tactacgccc gggacatggg ggtgcccatc caaaagctga tctgcgcctc
     5881 caacagcaac aatgtgctca ccgatttcct gtccaccggg acctatgacc gcaaccgcac
     5941 cttcttcaat accatctccc cctccatgga tatcctgatc tccagcaacc tggagcgcct
     6001 gctgctggcc atcactcagg aggacagcga ggtgcggggc tatatggaac agctgaatgc
     6061 caccggccgc tatcaggtga gcgacagggt gcgggagaag atccagaagc tgttcaaggg
     6121 ctactgctgc ggcgatgagg agaccaagcg ggtgatcgcc gccatccatg agaagttcgg
     6181 ctacctcatc gatccccaca ccgccgtggg cttctccgcg ctgagccagt accgggcgga
     6241 gacaggggat gagacggcgg ctattgtggc ctccaccgcc agccccttca agttctgcac
     6301 cgacgtgctg gacgccctgg gggtgtctga ccgggcccag ggcctggacg tgctggacca
     6361 gctcagccag gtcaccggcg agccggtccc cgcccctctg gccgcgctgc ggagcaagga
     6421 gtcccgcttc cgccagacgg tggagaagga gcacatggtg gacgccgtgc tggggatgct
     6481 ggagtaagga ggcgctatgg ccctgtacgc cattggggac acccatctct cgctgggtgc
     6541 ggacaagccc atggatgtgt tcggcggcgg ctggagcggc tatgtggaca agctccgggc
     6601 aggctttgca gagatcggtc cggaggatac ggtagtcctg tgcggcgacc tgtcctgggg
     6661 catgagcctg gagcaggccc gggaggactt tgccttcctg gacgcattgc ccggacggaa
     6721 gattctgctg aaggggaacc acgactactg gtggaccacc gccgccaaaa tggaacgctt
     6781 ttttgcagaa aacggtttct ccaccctgga gattctccac aacaactgcc gcctgtatgg
     6841 cggtgtggct ctgtgcggga cccggggctg gttctatgag gaggaccggg aggggcatgg
     6901 agcaaagatc ttcaaccggg agctgatccg tctggaggcg tcgctgaagg cggcggggga
     6961 gcgggagaaa tactgctttc tccactatcc gcctctctac cagggctacc gctgtcagga
     7021 gatcatcgat ctgctggagc ggtatcaggt cagccggtgt ttttacggtc atctccacgg
     7081 tggaagccac cgtctggcca tctccggacg gcagggcggg gtggaatatg acctggtttc
     7141 ggcggactat ttgggcttcc agccaaaaaa aattctggat tagcaaaaaa ttcacgaaga
     7201 attccgaaaa atggtctggt caaacagaca tatatctggt aaaatatcgt ggcgaaagta
     7261 tacggtgcgg cagagggcgg cccggaacgg tattccgggc cccgccttcc gcatccacag
     7321 gaggaaaagt tacatgaagg tcaccaacgt cgaaaagaaa gaaaacagca cggtcgagct
     7381 gacgatccat gtggaagccg acgagtttga ggctgcggtc cagaaggcct atctgaaaag
     7441 ccgctcccgc atcgccgtcc ccggcttccg gaagggcaag gccccccgca agatcattga
     7501 ggggatgtac ggatccggcg tgttttatga agatgccatc aacgatgtct atcccggcgc
     7561 ctatgaggcc gctgtgaagg agcagggcct ggaggatatg ggccaccccg tcatggatat
     7621 cgtggaggtc aacaaggaga agggcctgat ctttactgcc ctggtctctg tccgccccga
     7681 ggccaaggtg aaggcgtaca agggcctcac cgcccccaag gccgaggtca gcgtcaccga
     7741 tgaggatgtg gagaatgagc tgcagcccta tgtccgccgc gccacccgtc tgatctccgt
     7801 ggaccgcgag gtcaagctgg gtgacaccgc cgtcatcgac ttcgagggct tcgaggacgg
     7861 caagcccttc gagggcggca agggcgagaa ttacagcctg gagatcggct ccggctcctt
     7921 cgtccccggc ttcgaggatc agatcatcgg tctgaaggcc ggcgatgaga aggatctgga
     7981 tatcaccttc cccgaggatt atgtggccga tctggctggc aaggctgtgg tgttcaaggt
     8041 caaggtcaag gaggtcaagg agtcccagct ccccacggtg gacgatgagt tcgctaagga
     8101 tgtgtctgag tttgagactc tggctgactt taagaaggac ctgggcgaga agctgaccca
     8161 gcgccgcacc gccgaggccc agaacgactt cgagcaggcc attctggagc agctggtgga
     8221 caacctggag gctgatatcc ccaacggcat ggtggagacc cagcttgaca agatcatgga
     8281 cgagtacgcc atgcggatgt ccagccaggg gatgtccatg gacgactatc tgaagatgat
     8341 gggcatgacc cccgagatga tgcgcgcctc ctccaagccc gccgccctga accaggtcaa
     8401 gaccgagctg gctctggagg ccgtggccgc tgcggagaat ctggagatca ccgatgagga
     8461 gtgcgaggcc gaggtgaaca agctggccga gcagtacaag ctcaccgccg agcaggtgaa
     8521 ggccgccgtc tctctggacg ccctgaagca cgacctgcgg ctgcagaagg cctctgagct
     8581 ggtggtggcc gaggccaagg tgggcgaggc ccccaagaag gaagaggagg ccgccgagaa
     8641 gcccaagaag gcgacccgca agaagaagac cgaggaggcc gctgaggaga gcgagtctgc
     8701 ggagaagccc aagcgcaccc gcaagaagaa ggcggaggag tccgccgagg agcccaaggc
     8761 ggagtgatct gatccggtcc cggggcggcc ttgggccgcc ccgggcgtat ccgtctgtgc
     8821 cctctgcctg ttccaggcag acaggcagaa aagtggaatc attttccggc ccggcgggca
     8881 tactttggta tcaccgtctg aaaaggagta tatcgtatga gtttagttcc ctatgtagtg
     8941 gagcagacca cccgtggcga gcggtcctat gacatttttt cccgtctgct caacgaccgc
     9001 atcattttcc tgtctgagga ggtcaacgac accaccgcct ccctggtggt ggcccagttg
     9061 ctgtatctgg agagccagga tccggacaag gagatccagt tctacatcaa cagccccggc
     9121 ggctctgtga ccgcgggcat ggccatctat gacaccatgc agtatgtcaa gtgtgacgtg
     9181 tccaccatct gcattgggct ggcggccagc atgggcgctt tcctgctgtc tgcggggacg
     9241 aagggcaagc gtctggccct gcccaacgcg gagatcatga tccatcagcc ctctgccggc
     9301 acccagggcc agatcaccga catggcgctg cacctgaagc gcctggaggt agtgaagcgg
     9361 cggatgaacc gcatcctggc cgccaacacc ggccggagcg tggaggaggt caccgctgac
     9421 tgtgagcggg acaactttat gactgcggaa gaggcgcttc agtacggact gatcgacaag
     9481 gtcatcgaga agcggtgatc tgcaccgcgg agtgggggcc tgcgtcccca ctctgtttgt
     9541 attgttacga ggatgaggta gaaattcatg gcaaaaaatg acgataagca gcgcgcagtg
     9601 cgctgttcct tctgcggcaa gcaccaggat caggtccgcc ggctcatcgc tgggccgggc
     9661 gcctatatct gcaacgagtg cgttcagctg tgtatgagca tcctggagga tgagcgcgac
     9721 acggcccaga ctgccgccgc tgaggtgccc gacgccatcc ccaccccccg ggagatccat
     9781 aaggtgctgg accagtatat cattgggcag gagcaggcca aggtggccct gtccgtggcg
     9841 gtgtacaacc actataagcg gatctacttc ggcggcgggg acgatgtgga gcttcaaaag
     9901 agcaatatcc tgcttatggg gcccactggc tgcggcaaga ccctctttgc ccagactctg
     9961 gcccgggtgc tgaaggtccc cttcgccatc gccgatgcca ccaccctcac cgaggcgggc
    10021 tatgtgggtg atgacgtaga gaatatcctg ctgcgtctgg tccaggccgc cgactatgat
    10081 gtggagctgg ccgagcgggg catcatctat atcgacgaga tggataagat cgcccggaag
    10141 agcgagaaca cctccatcac ccgggacgtg tccggcgagg gcgtgcagca ggctctgctg
    10201 aagatcctgg agggcactgt ggccaacgtt cccccccagg gcgggcggaa gcacccccac
    10261 caggaattca tccagatcga cacgaagaac atcctgttca tctgcggcgg tgccttcgag
    10321 ggcatcgaca agatcatcga gagccgcctg gataagcgct ccatgggctt cggcgcagag
    10381 atccggagca agaaggagcg ggacgtgggc cagctgctta aggagatcca gccccaggat
    10441 ctgctgaaat tcggcatcat tcccgagctg gtgggccgcc tgccggtgat caccacgctc
    10501 cggtccctgg gacgggagca gatggtgcag atcctaaccg aacccaaaaa ctccctggtg
    10561 aagcagtacc ggaagttgat ggagtacgat gacgtggacc tggagttcac acccgaggcc
    10621 ctggaggctg tggccggccg ggcagtggag cggggcatcg gcgcccgcgg cctgcgggcc
    10681 atcctggagg aggtcatgac ccggattatg tacgatgtgc cctccgaccc cgaggtggtc
    10741 aaggtgacca tcactgagga ctgcatccgc agcggggcag cgccggagat ccttcgggac
    10801 ccggagcaca gccagcgccc ggctctgggc caggcggacc tgcgggctga acgcggcggt
    10861 ctgcccggta ccaacgcggg ctgagcggat cgctccggcc ggcgcagtaa gcattgatcc
    10921 agccccgcgc ccccccaagc ggcgggcccg gggctgttgt tttatcagcg ttccgccttt
    10981 cccacatcct ccgcacccag cgctggggag gagaaggcgg tatgaaatcc cctgagagga
    11041 agtgagtacc atgaaaaaag agtggacgat ggagagtgcc tctgtgatgc ctgtgatcgc
    11101 cctgcgggga ctgactgtgt tccccaatgt gctgatccac ttcgatgtag cccgggagat
    11161 ctccatcaag gccctggagg aggccatgtc ctccggcagc ccggtgttcc tggtgggaca
    11221 gaaggacatc tccgaggagc gcccggagga gaaggacctg tacactgttg gaacgatttc
    11281 caatgtgcgg cagatcctgc gcacccccgg cgacaacgtg cgggtgatgg tggagggcgt
    11341 gagccggggc agcctggagc ggctgacccg caccggcccc tatctggagg cggaggtcca
    11401 gcccatcccc agcgagcagc atgagcggaa ttccgccaag gcggaggcct tgatccgttc
    11461 cacctatgag ttgttccagc agtacaccga gctggccccc aagacggccc ccgacctgct
    11521 catcaatgtg ctggccagcg aggatccggg ctatattgcc gactttatcg cccaaaacat
    11581 cgccatgcgc aacagcgata agcaggcggt cctggaggag ctgcgcccgg tgcgccgcct
    11641 ggagcggctg caccgcctgc tgggccggga ggtggagatc ctggggctgg atatggagat
    11701 ccagaaccgg gcccgggagc agatggcgga caaccagcgg gactactatc tgcgggagca
    11761 gatgaaagcc atccagaacg aactgggcga gggtgagggc ggagactcgg agattgggga
    11821 ctaccgccgg aagatcgctg aggccaagct gcccgacgag gtgcgggaga agctggagaa
    11881 agaggtgggc cgtctggcca agcagccctt cggctcctcg gaggccaccg tgctgcgcag
    11941 ctatctggac gtctgcctgg agctcccctg ggggaaaaag acccgggagc gggtgagcgt
    12001 ggaggcggcc cgccggacct tggacaagga ccacttcggc ctggaaaagg tgaaggagcg
    12061 cattttggag ttcctggcgg tgaagcagct ggcccccgac ctgaaggggc aggtgatctg
    12121 cctggtgggc ccgcccggcg tgggtaagac gtcgctggcc atgtcggtgg cccgggcgct
    12181 gaaccggaag ctggcccggc tctccctggg cggcgtcagt gatgaggcgg agatccgggg
    12241 tcaccgcaag acctatgtgg gcgccatgcc cggccgcatc atctccgcca tcaatcaggc
    12301 gggaagctgc aatcccctgt tgctgctgga cgagatcgac aagctgggcc acgaccaccg
    12361 gggggatccg gcgtccgccc ttctggaggt gctggatgcg gagcagaaca gcaccttccg
    12421 ggacaatttt ttagaactgc ccttcgattt gtctgatgta atgttcatca ccacagccaa
    12481 taccaccgac accatccccc ggcccctgct ggaccggatg gaggtcattg aactgcccag
    12541 ctacaccgac gaggagaagc tccagatcgc caagcgctac ctcctgccca aggagatgaa
    12601 gcgccacggc ctggagaaag cccagctgaa ggtgtccgac ggggccatcc gggagatggt
    12661 cgcctcctac acccgggagt ccggcgtccg cgttctggaa cgcaagctgg ccgggatctg
    12721 ccggaaggcg gctatgcacc tggtgtcaga ggatgtaaag cagatccgca tcacggaaaa
    12781 agagctgaag gactatctgg gtgcgccccg gtactaccca gagcgccaag ctttggagga
    12841 gcgcgtgggc gtggtgaacg gcctggcttg gacctctgtg ggcggcgagc tgctggaggt
    12901 ggaggtcaat gtggtccccg gcaccggcaa ggtggagctg accggtaacc tgggggatgt
    12961 gatgaaggag agcgcccacg cggccctcag cttcatccgc agccaggcgg accggctggg
    13021 catccagggg gacttctacc agacgaagga catccatgtc cacttcccgg agggggctgt
    13081 gcc
//
LOCUS       DBMDSJ010000006        11284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_1946_length_11284_cov_1.694447, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000006 DBMDSJ010000000
VERSION     DBMDSJ010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 11284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11284
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_11284_cov_1.694447"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="ACZPAQ_00415"
     CDS             <1..1667
                     /locus_tag="ACZPAQ_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014647.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR0902465.1"
                     /translation="FSRGFYPILPADTEFAAKWSALCRIHLEEGIRDPVKVVEYLNHF
                     YVMEGHKRVSVLKYFGAITIPATVTRLLPKPSDDPEVAAYYEFLDFYKMTGLNFIVFR
                     QPGEYPELLEALGRSDSDAWSAEDIYSFRAFFYMFRDAYLRGDWDYTGISLAFLTYIK
                     IFGYQESCGKMPSQICEELSRIRPEIQNRLENAGTALLLEDDVKKPRFSFYTPGRVCA
                     AFIHDSSAAASKWVYSHEYGRHILENFTDGQVRTLSYENVRTDGEVDQAVEDAVRRGA
                     NVIFTTTPKLLLSSVRQAALHPDVKILNCSLNTSYPSVRTYYPRLYGAKFIKGAIAGT
                     LTRDGCIGYVADYPTYGSIASINAFAQGARMVNANARIYLDWAHLRDGGAVGRLQAQG
                     IVYIDYLDRLAASTGTQMGNRHNLALIQTHWGRLYQSLVRRVMEGSWKQEDQGSSAVN
                     YWWGMEQGVVSVLCSRRLPTGPRRLAGVLREALKADRLDPFYGVLMDQSGRVVYGDDA
                     PMPADRILSMNWLLTSVEGRIPQFEEFTEKAQELIKLQGVERQSEP"
     gene            1664..2254
                     /locus_tag="ACZPAQ_00420"
     CDS             1664..2254
                     /locus_tag="ACZPAQ_00420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0902466.1"
                     /translation="MKILAISDCESRALWDFYTPGKLEGLDLILSCGDLDPRYLSFLA
                     TFASCPILYVHGNHDGKYRETPPAGCLCVEDRLYEYQGVRILGLGGSMRYSRGPHQYT
                     EREMCMRALKLAPKLLWNRGFDILLTHSPGFGMVDCRDLAHTGFQTFNRLLDLFHPAL
                     FLHGHTHLSYNRGIRREEQYGSTRVVNCYERYLVEL"
     gene            complement(2336..4006)
                     /locus_tag="ACZPAQ_00425"
     CDS             complement(2336..4006)
                     /locus_tag="ACZPAQ_00425"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HIR0902467.1"
                     /translation="MDNQELNTHFIKVIGHRNPDTDSVCSAIAYSRLKNTLDPEHPCK
                     PCRAGLLNRETEFVLNYFQVPDPQLYTDVSPQIRDVDIRRMEGVDGEMSLRRAWITMR
                     DQQIDTLCVVDKERNLKGLITVKDVATANMDVLDTYILAESETSYQNIVDILDAKVLV
                     GDITGKKVEGRIVIGSGSAEQMERSISKGDIVIVANRSESQLTAIEMGAGCVVVCAGC
                     KVSRTICMLAEEQGCMIISTPNSTYVSGQMISQAAPIRHYMVRDDLLTFNLNSSVESA
                     TKVMASVRFRYFPVLDDDGHYIGVVSRRNMMNLHKKQLILVDHNEKGQAVEGIDQAEI
                     LEIIDHHRIGSMETDGPVYFRNVPVGCTCTIIYQMYRENGVELDRSTAGLLLSAILSD
                     TLMFRSPTCTPLDEQAGRELAKLTGVDLEQYANAMFEAGGDVTGRTAEEVFNTDYKIF
                     NSGEVRFGVGQGSYLTEKNRKASEALIGPYLQTALEKQGLDYIFYMFTDVRNSSTELL
                     MAGKGAESLVEKAFDTTVTDGMAVLPGVISRKKQMVPVLINAIKQERG"
     gene            complement(4223..6001)
                     /locus_tag="ACZPAQ_00430"
     CDS             complement(4223..6001)
                     /locus_tag="ACZPAQ_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HIR0902468.1"
                     /translation="MEHVSVNTVDVVNLKINGIACRAPAGATVLEAAHGAGIAIPTLC
                     YLKELNAIGACRICVVEVKGAKNLVPACVYPIADGMEVYTNTERVQAARRTNLKLILS
                     IHNQTCLTCSRSGLCELQRLCREYGVDNQMAFEGEKICYEPDTSAVHMVRDNSKCIMC
                     RRCEAVCSLAQGVACIGTSGRGFATHIGPSFDSPLSETACIHCGQCIIACPTGALYEK
                     DNTGLVWNALGDPQKHVVVQTAPSVRAGLGEMFSLPIGTNVEGKLAAALRRLGFNGVF
                     DTDFAADLTIMEEATEFLRRVQSGGTLPLITSCCPGWVKYCETFHPDFIPNLSTCKSP
                     QQMFGAMAKSYYAEKMGLRPEDIFVVSVMPCTAKKFEITREGECGAGAGIPDVDVALT
                     TRELGQMIQRAGLLFAELPEEEFDPALGIATGAGHIFGASGGVMEAALRTASELLTGK
                     ELEKVEFQEVRGAHGIKEAAYEIAGRTVRVCVLSGTANAGQVLEDIRSGKRAYDFIEI
                     MACPGGCVNGGGQPLQPSVIRNFRSVSAMRAGALYQEDQEMPLRKSHKSPLIQEVYEH
                     YLDQPGSQRAHQLLHTCYTDRSGQHR"
     gene            complement(6016..7812)
                     /gene="nuoF"
                     /locus_tag="ACZPAQ_00435"
     CDS             complement(6016..7812)
                     /gene="nuoF"
                     /locus_tag="ACZPAQ_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR0902469.1"
                     /translation="MYRSYVMICGGPNCTLLAGRQEKLAEEFEVQLKLAGLRKEVQVM
                     RGGCLGLCSSGPNVAVFPEGTIYSHVHVEDVAEIVSEHLLKGGRVDRLLYNENEATST
                     VRSLEETEFYKKQLRIALRNCGVIDPQNIDEYIARDGYAALEKCLTELTPDEVIQIVL
                     DSGLRGRGGAGFPTGLKWRFASGERGQVQKYVCCNADEGDPGAFMDRSVLEGDPHSII
                     EAMAIAGYAIGASQGYIYVRAEYPIAVKHLQIALEQARSYGLLGEDIFGTGFSFDLEI
                     KLGAGAFVCGEETALMTSIEGKRGEPRPRPPFPAVKGLFQRPTILNNVETYANICPII
                     LRGAEWFSSIGSEKSKGTKVFALGGRIQNTGLVEVPMGTTLRTIVEEIGGGIPHGKKF
                     KAAQTGGPSGGCIPAEHIDTPIDYDNLAAIGTMMGSGGLIVMDEDTCMVDIAKFFLEF
                     TVDESCGKCTPCRVGTKRLLELLTKITEGRGTMEDLDRIEELAAFIKSNSLCGLGQTA
                     PNPVLSTLRYFREEYREHILEKRCPAGVCKDLLTYSIDPVKCRGCTICAQNCPADAIK
                     GSVKNPHKIDTSKCIKCGSCYEKCRFGAVIRG"
     gene            complement(7818..8213)
                     /locus_tag="ACZPAQ_00440"
     CDS             complement(7818..8213)
                     /locus_tag="ACZPAQ_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR0902470.1"
                     /translation="MGQTEHKNIKIRSRKDLEAIRERTRPLLEVERGARIVVGMGTCG
                     LAAGARQVYEAMAEEIRRLGLEDVILEPVGCVGICQFEPVVEVYQPGGVLTTYVHMTP
                     EQGVRVVQQHIRGGNPLAEYTIGAVRSGR"
     gene            complement(8227..8718)
                     /locus_tag="ACZPAQ_00445"
     CDS             complement(8227..8718)
                     /locus_tag="ACZPAQ_00445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR0902471.1"
                     /translation="MKETKQGALPGTAAQARQLKAWLKENGEKPGAALPALQKAQEIY
                     GYVPREVQQLIADALHKPLTEIYGVSTFYSQFNLEPKGRNRISVCLGTACYIKGGGRI
                     YQLLQEKLGIGDGGCTEDGRFSLDACRCLGCCGMAPVMMVNEDVYGSLTGDELDAILA
                     KYE"
     gene            complement(9301..9486)
                     /locus_tag="ACZPAQ_00450"
     CDS             complement(9301..9486)
                     /locus_tag="ACZPAQ_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902472.1"
                     /translation="MEALVLVGGTLLVWTVGFWAVGQLDRFFKEKNAPCRREREGQTR
                     RKRKVLCRNAGKAEGQR"
     gene            9839..9916
                     /gene="kdpF"
                     /locus_tag="ACZPAQ_00455"
     CDS             9839..9916
                     /gene="kdpF"
                     /locus_tag="ACZPAQ_00455"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HIR0902473.1"
                     /translation="MILLGAIIVLMGGYLVYALVHPERF"
     gene            9968..>11284
                     /locus_tag="ACZPAQ_00460"
     CDS             9968..>11284
                     /locus_tag="ACZPAQ_00460"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIR0902474.1"
                     /translation="MLQLVLTVIIFMIIVIPIGNYLYHIASGQRTFADPVFDRVDGGI
                     FKVCGIHPDKGMNWKQYALALLLTNAVMVFVGYLVLRIQFLPIFNPNGIGAMEETLSF
                     NTIISFMTNTNLQHYSGESGLSYLSQMLVITYMMFVSAATGYAACIAFVRGLAGKSKD
                     NVGNFYADLVKVTTRVLIPLSIIGGMLLIWQGVPQNFDPNVTVTTIEGTQQDIAMGPV
                     AALEIIKHIGTNGGGFLGANSSTPIENPTIISDLVELYSMMILPGACVIMFGKMVKDR
                     RRKTASSSEHSATTQDLVKTSELVSKPSFTAKLYGSEGRTIFFAMGIIFLIGLSVCYW
                     SESQGNPALAKLGLDQSMGSMEGKEVRFGIAQSAMFTTTTTSFTTGTVNNMHDTLTPL
                     GGMIPLLHMMLNVVFGGKGVGLMNMIMYAILGVFIFGLMIGRTPEYL"
BASE COUNT         1976 a         3722 c         3169 g         2417 t
ORIGIN      
        1 ctttctcccg agggttctac cctatcctcc cggcggacac cgagttcgcc gccaagtgga
       61 gcgccctgtg ccgcatccac ctggaggagg gcatccggga ccctgtcaag gtggtggagt
      121 acctcaacca cttctatgtg atggagggcc acaagcgggt cagcgtgctg aagtatttcg
      181 gcgctattac tatcccggcc actgtcaccc gcctgctgcc caagccgtct gacgacccgg
      241 aggtggccgc ctattacgag tttctggact tttacaagat gacgggtttg aacttcatcg
      301 tcttccgcca gcccggcgag tacccggaac tgctggaagc actgggccgc tccgacagcg
      361 atgcctggag tgcggaagac atctactcct tccgggcctt cttttatatg ttccgggacg
      421 cctacctccg gggggattgg gactacacag gcatctccct ggcctttttg acctacatca
      481 agatcttcgg ctatcaggag tcctgcggca agatgccctc ccagatctgt gaggagctgt
      541 cccgcatccg gccggagatc cagaaccggc tggaaaatgc ggggaccgcc ctgctgctgg
      601 aagatgacgt gaaaaagccc cgcttctcct tctatacccc gggccgggtc tgcgccgcct
      661 tcatccacga cagcagcgcc gccgcctcca aatgggtgta cagccacgag tatggccggc
      721 acattctgga gaacttcacc gacggtcagg tgcgcaccct ctcctacgag aacgtccgca
      781 ccgatgggga ggtagaccaa gccgtggagg atgcagtgcg caggggcgcc aacgtcatct
      841 tcaccaccac cccaaagctg ctgctctcca gcgtccggca ggccgctctc cacccggatg
      901 tgaagatcct gaactgctcc ctgaacacct cctacccctc ggtgcgcacc tattaccccc
      961 ggctgtacgg ggccaagttt atcaaggggg ccattgccgg cactctgacc cgggacggct
     1021 gcatcggcta tgtagccgac taccctacct acggcagcat cgccagcatc aatgccttcg
     1081 cccagggagc ccggatggtg aacgccaacg cccgcatcta tctggactgg gcccatctcc
     1141 gggacggcgg cgcggtggga cgtctccagg cccaggggat cgtctatatc gactacctgg
     1201 accggctggc cgccagcacg ggcacccaga tgggcaaccg gcataacctg gccctgatcc
     1261 agacccactg gggccggctg taccagagcc tggtccgccg ggtgatggaa ggcagctgga
     1321 agcaggagga ccagggcagc agtgcggtca actactggtg ggggatggag cagggcgtag
     1381 tcagcgtgct gtgctcccga cggctgccca ccgggccccg gcggctggcc ggtgtactgc
     1441 gggaggccct gaaggctgac cggctggacc ccttctacgg cgtactgatg gatcagagcg
     1501 gccgggtggt ctatggcgac gacgccccca tgcccgccga ccgcattctg tccatgaact
     1561 ggctgctcac ctcggtggag ggccgcatcc cccaatttga agaatttaca gaaaaagctc
     1621 aggagctcat caagctccag ggtgtggagc ggcagagcga gccatgaaga tcttagctat
     1681 ttctgactgt gaatcccggg cactctggga cttctatacc cccggcaagc tggagggact
     1741 ggacctgatc ctctcctgcg gcgacctgga cccccgctat ctctccttcc tggccacctt
     1801 cgccagctgt cccatcctgt atgtccacgg gaatcacgac ggcaagtatc gggaaactcc
     1861 acccgccggc tgcttgtgtg tggaggaccg cctgtacgag tatcaggggg tacgcatcct
     1921 ggggctgggc ggctccatgc ggtacagcag ggggccccac caatatactg agcgtgagat
     1981 gtgtatgcgg gctctgaagc tggccccgaa gctgctctgg aaccgggggt tcgacatcct
     2041 gctgacccac tccccaggct ttggcatggt ggactgccgg gatctggccc ataccggctt
     2101 tcagaccttc aaccgcctgc tggacctctt ccacccagcc ctgttccttc atggacacac
     2161 ccacctctcc tacaacagag gcatccggcg ggaggagcag tatgggagta cccgggtggt
     2221 caattgctat gagcgttacc ttgtggagct gtgagacgga caagcctgcg ggcatagata
     2281 aaaacaggag cgcctagggt cccacagccc caggcgctcc tgttttttcg tgtattcagc
     2341 cccgctcctg cttgatggcg ttgatcagca cagggaccat ctgctttttc cggctgatca
     2401 cccccggcag gaccgccatc ccgtccgtca cggtggtgtc aaaggccttc tccaccaggg
     2461 attcagcgcc cttgcccgcc atcagcagct cagtactgga gttgcgcaca tcggtgaaca
     2521 tgtagaaaat gtagtccagc ccctgctttt ccagtgcggt ctggaggtag ggaccgatca
     2581 gggcctcgct ggccttccgg ttcttctcgg tcaagtagct gccctggccc acgccgaagc
     2641 ggacctcccc gctgttgaag atcttgtagt cggtgttgaa tacctcctct gcggtccggc
     2701 cggtgacgtc gcctcccgcc tcaaacatag cattggcgta ctgttccagg tccaccccgg
     2761 tcagtttggc cagctcccgg cccgcctgct catccagggg ggtgcaggtg ggactgcgga
     2821 acatgagggt gtccgaaagg atggcggaga gcagcagtcc ggcggtggac cggtccagct
     2881 ccaccccgtt ctcccggtac atctgataga tgatggtgca ggtgcaccca accggcacat
     2941 tgcggaaata gaccgggccg tctgtctcca tggagccgat gcggtggtgg tcgatgatct
     3001 ccaggatctc cgcctggtcg atcccctcca ccgcctggcc cttctcgttg tggtccacca
     3061 ggatcagctg ctttttatgc aggttcatca tgtttctgcg ggagactacg ccgatgtagt
     3121 gcccgtcgtc atccagcacc gggaagtagc ggaatcggac ggaggccatc accttggtgg
     3181 cggactccac ggagctgttc agattgaagg tgagcagatc gtcccgcacc atgtagtggc
     3241 ggatgggggc ggcctggctg atcatctgtc cggagacata ggtgctgtta ggggtgctga
     3301 tgatcataca cccctgctcc tcagccagca tgcagatggt ccgggacacc ttgcagcctg
     3361 cgcacaccac cacgcagccc gcccccatct cgatggcggt gagctggctc tcactgcggt
     3421 tggccacgat gacgatgtcc cctttggaga tggaccgctc catctgttcc gcgcttcccg
     3481 agccgatgac gatgcgcccc tccaccttct tgccggtgat gtctcccacc agcaccttgg
     3541 cgtccaggat gtccacgatg ttctgatagc tggtctctga ctccgccagg atgtaggtgt
     3601 ccaacacgtc catgttcgcc gtggccacgt ccttcacggt gatcaggccc ttcaggttcc
     3661 gctccttgtc caccacgcac agggtgtcga tctgctggtc ccgcatggtg atccaagccc
     3721 ggcgcaggct catctcccca tccacgccct ccatccgccg gatgtccaca tcccgaatct
     3781 gagggctcac gtccgtgtag agctgggggt ctgggacctg aaaatagttc agcacaaact
     3841 ccgtctcccg gttcagcagt cccgcccggc agggcttgca ggggtgctct gggtccaggg
     3901 tgttcttcag gcggctgtat gcaatggcgg aacagaccga gtccgtgtcc gggttccggt
     3961 gtccgatgac cttgataaaa tgggtattca gctcttggtt atccacaact tacctcactt
     4021 tcctccgctc cgctcctcac ggatgtgggc ggctcttgtc gtctctggtc cccattatac
     4081 catatcctgt ctccgggtga caggggaaat tgtgattttt tctgccgctc tcccaccggc
     4141 atctcctgaa tcttctaaca gcaagggccg cggaacctca gttccgcggc cctgcgcctt
     4201 tgttataggg tgcctcctcc cctcagcggt gctgtccgct tctgtctgtg tagcaggtat
     4261 ggagcagctg gtgggcccgc tgactgccgg gctggtccag atagtgctca tagacctcct
     4321 ggatcagcgg gctcttgtgg ctcttccgca ggggcatctc ctggtcctcc tggtacaggg
     4381 cgccggcccg catagcggac acactgcgga aattgcggat cacagagggc tggaggggct
     4441 ggccgccgcc gttcacgcac ccgcctgggc aggccatgat ctcgatgaag tcataggccc
     4501 gctttccgct tcggatgtcc tccagcacct ggcccgcgtt ggccgttccg ctgaggacgc
     4561 acacccggac agtcctgcct gcgatctcat aggccgcctc cttgatgccg tgggcgcccc
     4621 gcacctcctg gaactccacc ttctccagct ccttgccggt gagcagctcg ctggccgtgc
     4681 gcagtgccgc ttccatcacg ccgccggagg cgccgaagat atgccccgct ccggttgcga
     4741 tgcccagggc cgggtcgaac tcctcctccg gcagctccgc gaacagcagt ccggcccgct
     4801 ggatcatctg tcccagctcc cgggtggtca gggcaacatc cacgtccggg atgcccgcgc
     4861 cggcgccgca ctccccctcc cgggtgatct cgaacttttt cgctgtgcag ggcatcaccg
     4921 acaccacaaa gatatcctcc ggccgcagtc ccatcttttc ggcgtaatag ctcttggcca
     4981 tagcgccgaa catctgctga gggcttttgc aggtggacag gttggggatg aagtccgggt
     5041 ggaaggtctc acagtacttt acccagccgg ggcagcagct ggtgatcagg ggcagcgtgc
     5101 ccccggactg gaccctgcgc aggaattcgg tggcctcctc catgatggtc aggtcggccg
     5161 cgaaatcagt gtcaaacacg ccgttgaaac ccagccgccg gagggccgcc gccagtttcc
     5221 cctccacatt ggtcccgatg ggcaggctga acatctctcc cagccccgcc cggacactgg
     5281 gggccgtctg taccaccaca tgcttctggg ggtcacccag ggcgttccac accaggcccg
     5341 tgttgtcctt ctcatagagg gcaccggtgg ggcaggcgat gatgcactgt ccgcagtgga
     5401 tgcacgcggt ctcgcttagc gggctgtcaa agctggggcc gatgtgggtg gcgaagcccc
     5461 ggcccgatgt gccgatgcag gccaccccct gagccaggct gcacacagcc tcgcaccggc
     5521 ggcacatgat gcatttggag ttgtctcgga ccatatgcac cgcgctcgtg tccggctcgt
     5581 agcagatctt ctccccctcg aaggccatct ggttgtccac cccgtattcc cggcacaggc
     5641 gctggagctc acagagtccg cttcggctgc acgtcaggca ggtctgattg tggatggaca
     5701 ggatcagctt caggttggtc cgccgtgccg cctggacccg ctcggtattg gtgtacacct
     5761 ccatcccatc ggcgatggga tagacgcagg caggcaccag gttcttggcc cccttcacct
     5821 ccaccacgca gatccggcag gcgccgatgg cgttcagctc cttcagataa cacagcgtag
     5881 ggatggcgat ccccgctccg tgggccgcct ccaggacggt ggcgccggcg ggggcgcggc
     5941 aggcgatccc gttgatcttg aggttcacca catccactgt attcacactc acatgttcca
     6001 ttttcacact ccccctcatc cccggatcac cgcgccgaac cggcattttt catagcagct
     6061 gccgcacttg atgcacttgc tggtgtcgat cttgtgggga ttcttcacgc tccccttgat
     6121 ggcgtcggcc ggacagttct gagcacagat ggtacagccc cggcacttga caggatcaat
     6181 ggagtaggtc agcaggtcct tgcacacccc ggcggggcag cgcttctcca ggatatgctc
     6241 ccggtactcc tcccggaagt agcgcagggt ggacagcact gggttgggcg ccgtctgccc
     6301 caggccgcac aggctgttgc tcttaatgaa ggccgccagc tcctcgatcc ggtccagatc
     6361 ctccatggtg ccccgcccct cagtgatctt agtcagcagc tccaacaggc gcttggttcc
     6421 cacccggcag ggggtgcact ttccgcagct ctcgtccacc gtgaattcca ggaagaattt
     6481 cgcgatgtcc accatgcagg tgtcctcatc catcacgatc aggcccccgg agcccatcat
     6541 ggtcccgatg gcggccagat tgtcgtagtc gataggggtg tcgatgtgct ccgcggggat
     6601 gcatccgccg gaggggccgc cggtctgggc ggccttgaat ttcttcccgt gggggatgcc
     6661 gcccccgatc tcctccacga tagtgcgcag ggtggtgccc atgggcacct ccaccaggcc
     6721 ggtgttctgg atacgcccgc ccagggcaaa taccttggtc cccttgctct tctcgctgcc
     6781 gatggaggag aaccactccg ccccccgcag aatgatgggg cagatgttgg cgtaggtctc
     6841 cacgttgttt aggatggtgg gccgctggaa caagcccttc accgccggga agggcggccg
     6901 gggccggggc tctccccgtt tcccttcgat ggaggtcatc agggcggtct cttccccgca
     6961 tacgaaagcc cccgccccca gcttgatctc caggtcgaag gagaagcccg tcccgaagat
     7021 atcctccccc agcagtccgt agctcctggc ctgctccaga gcgatctgga ggtgcttcac
     7081 cgcgatgggg tactccgccc ggacgtagat atagccctgg ctggccccga tggcatagcc
     7141 cgcgatggcc atggcctcga tgatactgtg ggggtcgccc tccagcacgc tccggtccat
     7201 gaaggccccg gggtcgccct cgtccgcgtt gcagcagaca tatttctgta cctggccccg
     7261 ttccccgctg gcaaagcgcc acttcagtcc ggtggggaag cctgctccgc cccggccccg
     7321 caggccggag tccagcacga tctggatcac ctcgtccggc gtcagttcgg tcaggcactt
     7381 ctccagtgcc gcatagccgt cccgggcaat gtactcgtca atgttctggg ggtcgatgac
     7441 gccgcagttg cgcagggcaa tgcgcagctg cttcttgtag aactctgtct cctccaggct
     7501 gcggactgtg ctggtggcct cgttctcgtt gtagagcagg cggtccaccc ggccgccctt
     7561 gagcagatgc tcactgacga tctccgccac gtcctccaca tggacatggc tgtagatcgt
     7621 gccctccggg aacactgcca cgttgggccc gctggagcac agccccagac agccgccccg
     7681 catcacctgg acctctttac gcagaccggc cagcttcagc tggacctcga attcctccgc
     7741 cagcttctcc tgcctgcccg ccagcagggt gcagttgggc cccccgcata tcatcacata
     7801 gctgcgatac attcccttca cctcccgctt ctcacggccc cgatggtgta ctccgccagc
     7861 gggttgccgc cccggatgtg ctgttgcacc acccgcacgc cctgctccgg cgtcatgtgc
     7921 acataggtgg tgagcacgcc tccgggctgg tacacctcca ccacaggctc aaactgacag
     7981 atccctacac agcctaccgg ctccaggatc acgtcctcca gccccagtcg gcggatctcc
     8041 tccgccatgg cttcatagac ctgcctggcc cccgcggcca ggccgcaggt gcccatgccc
     8101 accacgatcc gggctccccg ctccacctcc aggaggggcc tggtccgttc ccggatggcc
     8161 tccaggtcct tccggctgcg gatcttaatg tttttgtgtt ccgtctgccc cacgctcagt
     8221 ccctccttac tcatatttcg ccagaatggc gtccagctcg tcgccggtca ggctgccgta
     8281 cacgtcctcg ttcaccatca tcaccggcgc catcccgcag cagcccaggc agcggcaggc
     8341 atccaagctg aagcggccgt cctcggtgca gcccccgtct ccaatgccca gtttctcctg
     8401 gagcagctga tagatccgcc cgccgccttt gatgtagcac gcggtcccca gacacacgct
     8461 gatccggttg cgccccttgg gttccagatt gaactgggag taaaaggtgg acaccccata
     8521 gatctccgtc agcggcttgt gcagggcgtc cgcgatcagc tgctggacct cccgggggac
     8581 atagccgtat atctcctgcg ccttctggag ggcaggcagc gccgcccccg gcttctcccc
     8641 gttctccttc agccatgcct tcagctgcct ggcctgcgcc gccgtcccgg gcagcgcccc
     8701 ctgttttgtc tccttcatcc gtttgctcct cctgtcttgt cccgcggcag tccgcgtttt
     8761 tgcaagtttc cctgcttttt tgaagtgacg catttcctgc atcctctgtt aaaaaatcta
     8821 cacttcccca aataacacag aaagcggtcc tggtcccttt tctgaaccag cccgctttct
     8881 gtattttcac caatccaggc gaaatacgac acagtttata tgttgatatt accatattcc
     8941 attgtcgatt gcaatctctt ttttgtccac tcccggcccc cccttttcct ccaggaaaaa
     9001 cagcatcaga ctcaggcaga gcagatccgc gcacccgccc ggcttccgcg tggcggacat
     9061 ctcatagagc aggtcctgca cccccagggc accgcaaagt gggcgtttaa gctgcccaca
     9121 ccgctgccgg tcagcaacgg gggcagtgcg aattgaagcc gctggagcgg cccggcccat
     9181 cttccgcgga aaatcgatac agcaaggcca gacttttttg tttctgcggt cgtagacctg
     9241 tttcgaatac aggaggggcc cagactatgt aaaaacacag tctgggcccc tccccttgtc
     9301 ttacctctgt ccctccgctt ttcccgcgtt ccggcacaga acttttcttt ttctgcgggt
     9361 ctgcccctcc cgctccctcc ggcagggagc gtttttctcc ttgaaaaagc ggtccagctg
     9421 gcccaccgcc cagaatccga cggtccagac aagcagcgtc ccgcccacca gtaccagagc
     9481 ttccacaaga tcacttcctt aaaaatgatt ctactacaag ccctgtttgt gtgagatttg
     9541 caaatcatcc gccgtattaa aattgtatta ggagccaatt gtccttcttt ctttttatct
     9601 tttttatttc tttttcttaa tgcaagttta attctcctgt gaatacgtta accgcctgct
     9661 aatcccctgc gtgctataat acgtccgttc cggaaacaga actttggaaa ggatggtttc
     9721 tatgaaagac gtcatcatga tcgcggtctt attggtctgc ttcggatcag taaagctgct
     9781 ggctgactgg tgtgagcatc agttccacga ggaatgagag aaggtattga gtatgggtat
     9841 gattttatta ggtgcgatca tcgtgctgat gggcggctat ctggtttacg ccctggtaca
     9901 ccctgaacgt ttctgacccc aaatgtgtca tttgtaaagc gagtttcgca caggaggaac
     9961 aacacatatg ttacagcttg tcctaacagt gatcatcttc atgatcattg tcatcccgat
    10021 cgggaactat ctgtatcata ttgcttccgg ccagcgcacc ttcgccgatc cggtgttcga
    10081 ccgggtggac ggcgggattt tcaaggtgtg cggcattcat cccgacaagg gcatgaactg
    10141 gaagcagtat gcgctggctc ttctgctgac caacgccgtg atggtgtttg tcggctatct
    10201 ggtcctgaga attcagtttc ttcccatctt caaccccaac ggcatcggcg cgatggagga
    10261 gaccctgtcc ttcaacacaa tcatctcctt catgaccaac accaacttac agcactactc
    10321 cggtgagtcc ggcctgtctt atctcagcca gatgctggtc atcacttaca tgatgttcgt
    10381 gtccgccgcc actggctacg ccgcctgcat cgcctttgtc cgcggactgg ctggcaagag
    10441 caaggacaac gtgggcaact tctatgctga tttggtcaag gtgaccaccc gcgttctgat
    10501 tcctctttcc atcatcggcg gcatgctcct gatctggcag ggcgtccccc agaatttcga
    10561 tcccaacgtg acggtgacca ccattgaggg gactcagcag gatatcgcca tgggccccgt
    10621 ggccgcgctg gagatcatca agcacatcgg caccaacggc ggcggcttcc tgggtgccaa
    10681 ctcctctact cccattgaga accccaccat catcagcgat ctggtggagc tctactccat
    10741 gatgatcctg cccggcgcct gcgtgatcat gttcggcaag atggtcaaag accgccgccg
    10801 taagaccgcc agctccagtg agcacagcgc caccacccag gatctggtca agaccagcga
    10861 actggtgagc aagcccagct tcaccgcgaa gctttacggc tccgagggcc gcaccatctt
    10921 cttcgccatg ggcatcatct tcctcatcgg cctgagtgtc tgctactggt ctgagagcca
    10981 gggcaacccg gccttggcca agctggggct ggatcagtcc atgggtagca tggagggcaa
    11041 ggaagtccgc ttcggcattg cccagtccgc catgttcacc accactacca cctcttttac
    11101 tactggcacg gtcaacaaca tgcatgatac tctgactccc ctgggcggca tgattcccct
    11161 gctgcacatg atgctcaatg tggtgttcgg cggcaagggc gtgggcctga tgaacatgat
    11221 catgtatgct attctcggcg tgttcatctt tggcctgatg atcggccgta ccccggagta
    11281 cctg
//
LOCUS       DBMDSJ010000007        10717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2051_length_10717_cov_1.484081, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000007 DBMDSJ010000000
VERSION     DBMDSJ010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2051_length_10717_cov_1.484081"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="clpX"
                     /locus_tag="ACZPAQ_00465"
     CDS             <1..979
                     /gene="clpX"
                     /locus_tag="ACZPAQ_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR0902475.1"
                     /translation="ILLMGPTGCGKTLFAQTLARILKVPFAIADATTLTEAGYVGDDV
                     ENILLRLVQAADYDVELAERGIIYIDEMDKIARKSENTSITRDVSGEGVQQALLKILE
                     GTVANVPPQGGRKHPHQEFIQVDTKNILFICGGAFDGIDKIIENRLDKRSMGFGAQLQ
                     SKKERNVGEFLKEIQPQDLLKFGIIPELVGRLPVITTLQSLDREQMIQILTEPKNALV
                     KQYQKLLDYDEVVLEFSREALEAVADRAVARGIGARGLRAVLEEVMTKIMYSVPSDPT
                     IVKVTITASCVTDGADPEIVRDPERRQRPRLGKASLRAERGGAVGTNAG"
     gene            1079..3493
                     /gene="lon"
                     /locus_tag="ACZPAQ_00470"
     CDS             1079..3493
                     /gene="lon"
                     /locus_tag="ACZPAQ_00470"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR0902476.1"
                     /translation="MDHDPVASIQTLPAIALRGLTVFPNVLIHFDVGRAASIKAMEAA
                     MAAGEPVFLVGQKDMTVERPERDDLYTVGTLSNVRQILRMPGDNVRVMVEGISRARLR
                     DVTQREPYLMAEIEAIPEEKAGRASAKTEALMRSTYEMFQNYTELAPKISPDLLINVL
                     ASDDPGYIADYIAQNIAMRNSDKQAILEELRPVRRLERMYRLLCREVEVLSLDMEIQS
                     RAREQMSENQRDYYLREQMKAIQAELGESENGDSETADYRKKIAEAQLPEPVREKLEK
                     ELNRLSKQPFGSSEATVIRNYLDVCLELPWGKKTRERVSVEAARKVLDKDHYGLEKVK
                     ERILEFLAVKQLAPELKGQVICLVGPPGVGKTSIAMSMARAMNRKLARISLGGISDEA
                     EIRGHRKTYVGAMPGRIINAISQSGSSNPLLLLDEIDKLGYDHRGDPASALLEVLDGE
                     QNASFRDHFLEVPFDLSDVLFVTTANTTETIPRPLLDRMEVIELNSYTDEEKLQIAKR
                     HLLPKELKRHGLARTQLKVSDGAIREMVASYTRESGVRVLERKMATLCRKAAMKLVEG
                     TCKSVSITERDLEEYLGAPRFHPEREALAQRVGVVNGLAWTSVGGELLEVEVNVVPGS
                     GKVELTGNLGDVMKESAHAALSFIRSRASQLGIDGDFYKNRDIHVHFPEGAVPKDGPS
                     AGIAITTAMVSALTDTPVKPGLAMTGEVTLRGRVLAIGGLREKTMAALRNGIKTVIVP
                     AENEKDLEEIDQTVRAALHFILVEQVDQVLAAALSLSSTACERDTEQGGPFPAGKGRT
                     SIGLKQ"
     gene            3508..4095
                     /gene="yihA"
                     /locus_tag="ACZPAQ_00475"
     CDS             3508..4095
                     /gene="yihA"
                     /locus_tag="ACZPAQ_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR0902477.1"
                     /translation="MAINFQQAEFIRSATAPKDFPRDGLPQVAFAGRSNVGKSSVINR
                     LLGRKNFARVGAAPGKTVHINYFLIDKTFYLVDLPGYGYAKVSKAERDRWGRLMEDYF
                     ADRELMTFGAMIVDARHKPTADDCTMAQWYRDAGCPFVVVANKLDKLKKREIESNLLT
                     IRETLELDDSVKLIPFSAEKGEGKQELLDLILSKL"
     gene            4185..4997
                     /locus_tag="ACZPAQ_00480"
     CDS             4185..4997
                     /locus_tag="ACZPAQ_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0902478.1"
                     /translation="MLERSYQTRQGTIRYWVSAPGPRGTALVFLPGLTADHRLFQMQF
                     SFFASRRPLLVWDAPGHGASRPFALDFSLMEQAEWLHGILVRERMGGPVLIGQSMGGY
                     LAQCFMERFPGQARGFISIDSAPLQRRYVTAAELWALRRCELLYRCYPWSILRKAGAR
                     GCAETAQGQTLMKEMMDCYSKREFSALAGHGFRALAEAMAADLPYKVDCPALLLCGER
                     DRAGLTRRYNRRWSEETGIPLVWLQSAGHNSNTDVPDQVNSIIQGFLREHGL"
     gene            5245..5931
                     /locus_tag="ACZPAQ_00485"
     CDS             5245..5931
                     /locus_tag="ACZPAQ_00485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR0902479.1"
                     /translation="MGSLNGFLEHMDWFQLFLLLIGAVACLFCITFHELAHGFAAYQL
                     GDPTAKVNGRLTLNPISHIDWIGLMLLLTVRVGWAKPVPVDMRNFRHPKRDMAITALA
                     GPAANFLLATAALGVASLIYHFAPQGTVAAYAIYFFLYMAVLSVGLGIFNLVPIPPLD
                     GSKILFALLPDRIYYQYLRYERFFILAVVLLAWMGVFSVPLSFCMEGIVRGLCAVTAF
                     PFQLAQYYFF"
     gene            5952..6758
                     /locus_tag="ACZPAQ_00490"
     CDS             5952..6758
                     /locus_tag="ACZPAQ_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR0902480.1"
                     /translation="MDSPIYHLEGVVKAKTEDMEDFVGPLDLILHLLSKNKMEIKDIQ
                     ISLILDQYLAWMAARKEMDLEVASEFVTMASQLVYIKTRMLLSIHDEEALSEMEQLIA
                     SLEEHQRNENYLKIKEITPLLDRRYSYGRDFITKVPETIQPDRTYRYVHDREDLKKAI
                     LSVLSRADHQLPPPVAAFQGIVGREPYPVSEKAGEIIRRLLRTGVTRFRALFQGNRSR
                     SEVVATFLAVLELCKAHRLRLAGTEEDCTITSTQGEQGDHLEVTADSFEF"
     gene            6777..7412
                     /gene="scpB"
                     /locus_tag="ACZPAQ_00495"
     CDS             6777..7412
                     /gene="scpB"
                     /locus_tag="ACZPAQ_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR0902481.1"
                     /translation="MELKELEAALEGVLFAAGEPVGVERLCLGLEVDRATMDAVAQRL
                     MDRYSYERRGIRLVRLDTSYQLCSAPEFAPYIRKTLENRKPARLSQPALEVLAVIAYY
                     QPVTRAYVDQIRGVDSAYTVGLLLERELIEECGRLAVPGRPILYRTTKNFLRSFGLSS
                     LEELPELPDASQESGQLTLELEAAVARLRAEESGQTVQEDGAAHPAQEETE"
     gene            7409..8017
                     /locus_tag="ACZPAQ_00500"
     CDS             7409..8017
                     /locus_tag="ACZPAQ_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HIR0902482.1"
                     /translation="MRGWIVLAAVLLLLFLLGQLRLGAVGEYGPGGAGAWFRAGPVRI
                     RLYPRRRSGPSKKEKAPKPGKEHPAGEARGGGLLELMKELLPLALEAAGCFRRRLRVD
                     QLELKLTIGAADPGDAAIRYGQANALLGSIWQPLTEAFHVEEGRARVEVDFTAAAPVL
                     TGQVSLSLKLYQLLWLGLHFGLRALSTYVNHRNRHKQQREAV"
     gene            8020..8445
                     /gene="ytfJ"
                     /locus_tag="ACZPAQ_00505"
     CDS             8020..8445
                     /gene="ytfJ"
                     /locus_tag="ACZPAQ_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR0902483.1"
                     /translation="MEKQHPINEMMSTTMEKIRSMMDANTIIGDPIQTGEVTLIPVSR
                     LSFGFASGGSDFVTKNQKPGAENTFGGGSGASAKMEPVAFLIIKGDSVKLLPVDPPPA
                     TTVDRVIEVVPEVVEKVTDFLEKQKKADEPPEGTDHFVV"
     gene            8544..9644
                     /locus_tag="ACZPAQ_00510"
     CDS             8544..9644
                     /locus_tag="ACZPAQ_00510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0902484.1"
                     /translation="MKRLLAAALAAGSMLCQVPTAQAVGTSASSAILMEAESGRVLYE
                     QNAHEERLIASITKLMTALVALESGHDLEETVTVGEACTRAEGSSLYLRPGEEISLKG
                     LLYGVMLRSGNDAALAVAEHCGGSVEEFVGRMNEKAAQLGMVNSHFANPNGLNAEGHY
                     SSAYDMALLARACLENEALAEIAATKSATVDGRVLTNHNKLLWQYEGCIGLKTGYTEK
                     AGRTLVSAARRGGMTLIAVTLNDPDDWRDHTALLDYGFSTYAPVTLTEEGKAVARRPV
                     SGSLVPFVEIQAAETVRIPVCEEDKIDWELQWDQEALEAPLRAGGSAGRMVCRVNGEE
                     AACVPLVFAQDVDRALQPPGGIWTRLMELWNR"
     gene            9667..10380
                     /locus_tag="ACZPAQ_00515"
     CDS             9667..10380
                     /locus_tag="ACZPAQ_00515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR0902485.1"
                     /translation="MEERLQKLLSACGLASRRTVEQWIEAGRVTVNGTKARLGDKADL
                     DRDRIEVDGRPVRPGGDRLYLMLNKPRGYVTTLSDEKGRKTVAELVSGCGRRVWPVGR
                     LDLDSEGLLLLTDDGELTQKLLHPSHQVEKEYLVWVTGDVSRGLPVLSGPMDLDGVPL
                     SPAKVRRGRTSGGVTQLSITICQGKNRQVRRMCGQAGLKVLRLKRVREGGLCLDRTLG
                     PGKWRRLEQSEVASLLQKP"
     gene            10530..>10717
                     /locus_tag="ACZPAQ_00520"
     CDS             10530..>10717
                     /locus_tag="ACZPAQ_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR0902486.1"
                     /translation="MNRDILTVIQENMSTFSKGQKRIANYILESYDKAAFMTASRLGK
                     TVSVSESTVVRFAAELGY"
BASE COUNT         2100 a         3073 c         3644 g         1900 t
ORIGIN      
        1 catcctgctc atgggcccca ccggctgcgg caagaccctg tttgcccaga ccctggcccg
       61 aatcctgaag gtgcccttcg ccattgccga cgccaccact ctgaccgagg cgggctatgt
      121 gggcgacgac gtggagaaca tcctgcttcg tctggtgcag gccgccgact acgacgtgga
      181 gctggcggag cggggcatca tctatatcga cgagatggat aagatcgccc ggaagagcga
      241 gaacacctcc attacccggg acgtttccgg tgagggcgtg cagcaggccc tgctgaagat
      301 cctggagggc acggtggcca atgttccgcc ccagggcgga cggaagcacc cccaccagga
      361 gttcatccag gtggacacca agaacatcct gttcatctgc ggcggcgcct tcgacggcat
      421 cgacaagatc atcgagaacc gcctggataa gcgcagcatg ggcttcggcg cccagcttca
      481 gagcaagaag gagcgcaatg tgggcgaatt cctgaaggag atccagcccc aggacctgct
      541 gaaattcggc atcatcccgg agctggtggg ccgcctgccc gtcatcacta ccctccagtc
      601 actggaccgg gagcagatga tccagatcct cactgagccc aaaaacgcct tggtgaagca
      661 gtatcagaag ctgctggact atgacgaggt ggtgctggag ttcagccggg aggccctgga
      721 ggcggtggcc gaccgggcgg tggcccgggg catcggagcc cggggcctgc gggccgtgct
      781 ggaggaggtc atgaccaaga tcatgtacag cgtcccctct gaccccacca tcgtcaaggt
      841 gacaatcacg gcttcctgcg tgacggacgg ggccgacccg gagatcgtcc gggacccgga
      901 gcgccgccag cgcccccgtc tgggcaaggc gtccctgcgg gccgagcgcg gcggtgcggt
      961 gggtaccaac gccggctgaa tcccatttga atgaccattg ctgcaatgtc ccgcggaagc
     1021 ctatcctgtg cgggcctccc gggacttgct ttttattccc agagaggaag tgacgaacat
     1081 ggaccatgat cctgttgcca gcatccagac tctcccggct atcgcgctgc gggggctgac
     1141 tgtgtttccc aatgtactga tccacttcga cgtgggccgc gcggcctcca tcaaggcgat
     1201 ggaggccgcc atggctgccg gagagccggt gttcctggtg ggccagaagg acatgacggt
     1261 ggagcgcccc gaacgggatg acctgtacac agtgggcacc ctgtccaacg tgcggcagat
     1321 cctccgcatg cccggggaca acgtgcgtgt gatggtggag ggcatctccc gggcccgcct
     1381 gagggacgtg acccagaggg agccctattt gatggcggag atagaagcca tcccagagga
     1441 gaaggcgggc agggcctcgg ccaagaccga ggccctgatg cgcagcacct atgagatgtt
     1501 ccaaaattac acggagctcg cccccaagat ctcaccggac ctgctcatca atgtgctggc
     1561 cagcgacgac ccaggctata tcgcggacta tatcgcccag aacatcgcca tgcgcaacag
     1621 tgacaagcag gccatcctgg aggagctccg gccggtgcgc cgcctggagc gtatgtaccg
     1681 cctgctgtgc cgggaggtgg aggtgctctc ccttgacatg gagatccaga gccgggcccg
     1741 ggagcagatg tcggagaacc agcgggacta ctatctccgg gagcagatga aggccatcca
     1801 ggccgagctg ggtgagagcg agaacgggga cagcgagacg gcggactatc ggaaaaagat
     1861 tgctgaggcc cagctgccgg agccggtgcg ggagaagctg gagaaggaac tcaaccgcct
     1921 gtccaagcag cccttcggct cctcagaggc caccgtgatc cgcaactatc tggatgtatg
     1981 cctggagctg ccctggggca aaaagacccg ggaacgggtc agcgtggagg ctgcccgcaa
     2041 ggtgctggac aaggaccact atgggctgga gaaggtgaag gagcgcatcc tggagttcct
     2101 ggccgtcaag cagctggccc ctgagctgaa ggggcaggtg atctgcctgg tgggcccgcc
     2161 tggtgtggga aagacctcca ttgccatgtc catggcccgg gccatgaatc gtaagctggc
     2221 ccgcatctct ctgggcggca tcagcgacga ggcggagatc cggggccacc gcaagaccta
     2281 tgtgggggcc atgccgggcc gtatcatcaa cgccatcagc cagtctggga gcagcaaccc
     2341 cctgctcctg ctggacgaga tcgacaagct gggctatgac caccggggtg acccggcctc
     2401 ggccctgctg gaggtgctgg acggagagca gaacgcctcc ttccgggacc acttcctgga
     2461 ggtgcccttc gacctgtccg atgtgctgtt cgtgaccact gccaatacca cagagaccat
     2521 cccacggccc ctgctggacc ggatggaggt catcgagctg aacagctata ccgacgagga
     2581 gaaacttcag atcgctaagc gccacctact gcccaaggag ctcaagcgcc acgggctggc
     2641 ccggacccag ctgaaggtgt ctgatggcgc catccgggag atggtggcct cttacacccg
     2701 ggagtcggga gtgcgggtgc tggagcgcaa gatggccacc ctgtgccgga aggccgccat
     2761 gaagctggtg gagggaacat gcaagtctgt ttccattaca gagcgggacc tggaggaata
     2821 tttgggagcg ccccgcttcc acccggagcg ggaggcactc gcccagcggg tgggcgtagt
     2881 taatggcctg gcctggacct ctgtgggcgg tgagctgctg gaggtggagg tcaacgtggt
     2941 ccccggcagc ggcaaggtgg agctcaccgg caacctgggc gacgtgatga aggagtcggc
     3001 ccacgcagcc ctcagcttta tccgcagccg ggcctctcag ctgggcattg acggagattt
     3061 ctacaagaac agggacatcc atgtccactt ccccgaaggg gcggtcccaa aggacggccc
     3121 ttccgcgggc atcgccatca ccacggctat ggtgtccgct ttgacagata caccggtaaa
     3181 gccgggactg gccatgaccg gcgaagtaac gctgagaggc cgggtgctgg ccatcggcgg
     3241 actgcgggaa aaaactatgg cggccctgcg caacggcatc aagaccgtga tcgttccggc
     3301 ggagaatgag aaggacctgg aggagatcga ccagacggtc cgcgccgccc tccattttat
     3361 cctggtggag caggtggacc aggtgctggc cgcagcgctg tctctgtcca gcaccgcctg
     3421 tgagcgggac accgagcagg ggggaccctt ccctgcagga aagggcagga cgtccatcgg
     3481 cctgaagcag taagaggggg agagaccatg gccatcaact tccaacaggc ggagttcatc
     3541 cgctccgcca ccgcccccaa ggattttccc agagacgggc tcccccaggt ggcttttgcc
     3601 ggacgctcca atgtggggaa gtcctcggtt attaaccggt tgctggggcg caaaaacttt
     3661 gcccgggtgg gggcggcccc gggcaagacc gtccatatca actactttct gatcgataag
     3721 accttttatc tggtggatct gcccggctat ggctacgcca aggtctccaa ggcagagcgg
     3781 gaccggtggg gccgcctgat ggaggactac ttcgcggacc gggagctgat gaccttcggc
     3841 gccatgatcg tggacgcccg tcacaagccc accgccgatg actgcaccat ggcccagtgg
     3901 taccgggacg ccggctgccc ttttgtggtt gtggccaaca agctggataa gctgaaaaaa
     3961 agagagatcg agtccaatct tctgacaatt cgagagacac tggagctgga tgattctgta
     4021 aagttgattc ccttttctgc tgagaagggc gaaggaaaac aggagcttct ggatttgatc
     4081 ctatcgaaat tgtgaggaag cacggcccct ctgcgcccat gcgggcacgg aggggccgtg
     4141 tgcgtgttcc cgctgcggcg gggagaaaag ggagggaaac ggagatgttg gagcggagct
     4201 atcagacgag acaggggacc atccgatact gggtgagcgc gcccggccct cgcgggaccg
     4261 cgctggtctt tctgcctggc ctgacggcgg atcaccggct gttccagatg cagttctcct
     4321 tttttgcatc ccgccggccc ctgctggtgt gggacgcacc gggccacggg gcctcccgcc
     4381 catttgccct ggatttctcc ctgatggaac aggcagagtg gctccacggg atcttggttc
     4441 gggagaggat gggagggcct gtattgatcg gtcagtccat gggggggtat ctggcgcagt
     4501 gctttatgga gcgattcccc ggacaggccc ggggatttat ctccattgat tccgccccgc
     4561 ttcaacggag gtatgtcacg gcggcagagc tctgggcact gcggcgctgc gagctgctct
     4621 accgctgtta cccctggagc atactgcgga aagccggagc cagagggtgc gcagagacag
     4681 cccagggtca gacgctgatg aaggagatga tggactgtta cagcaaaagg gaattcagcg
     4741 ccctggcggg gcacgggttt cgggctctgg cggaggcgat ggcggctgat ctgccgtaca
     4801 aagtggactg tcccgccctt ctgctgtgcg gggaacggga ccgggcgggc ctgacgagac
     4861 ggtataaccg ccgctggtcg gaagagaccg gcatccccct ggtgtggctc cagagcgcgg
     4921 ggcacaactc caacacggac gtgccggatc aggtcaactc catcatccag ggcttcctca
     4981 gggaacatgg actttgaaac gtgtcggcgg ggacctggcc cggtggggag gcagaggccg
     5041 cccttctacg gcagatccca gtcatcctca aaaggaaaaa tcttaccctc cggctttctg
     5101 taaatttccg aagttctctc tacgcagcat tttttgaaaa agagctgcct gattcgccgt
     5161 cttgtatttt ctttgagaat ctggtatgat aaacaagtat ttatttgtcc atgggtgccc
     5221 catggacagg gaagggaggc cgccatgggg tcgctgaatg gttttctgga gcatatggac
     5281 tggtttcaac tgtttctgct gctgatcggt gcggtggcct gcctgttctg catcaccttc
     5341 cacgagctgg cccacgggtt cgcggcctac cagctggggg accccaccgc caaggtgaac
     5401 ggccgtctga cgctgaaccc gatctctcat atcgactgga tcggcctgat gctgctgctc
     5461 actgtgcggg tgggctgggc caagccggtg ccagtggata tgcggaactt ccgccaccca
     5521 aagcgggaca tggccatcac cgccctggcc ggccctgcgg ccaacttcct tctggccacg
     5581 gctgccctgg gggtggcctc cctgatctat cacttcgcgc ctcaggggac ggtggcggcc
     5641 tatgccatct atttcttcct gtatatggcg gtgctcagtg tagggctggg gatctttaac
     5701 ctggtcccta tcccaccgct ggacggttcc aagatcctgt tcgccctgct gcccgaccgg
     5761 atctactacc aatacctgcg ctatgagcgg ttttttatcc tggctgtggt gctgctggcc
     5821 tggatggggg tgttcagcgt gcccctgagc ttctgcatgg agggcatcgt ccgcggcctg
     5881 tgcgcggtga ccgccttccc gttccagctg gcgcagtact atttcttctg agaggcattg
     5941 gggggaacgc tttggacagt ccgatctacc acctggaagg ggtggtcaag gccaaaacag
     6001 aggacatgga ggactttgtc ggccccctgg acctgatcct tcacctgctg agcaaaaaca
     6061 agatggagat caaggacatt cagatctctc tgatcctgga ccagtatttg gcctggatgg
     6121 ccgcccgcaa ggagatggac ctggaggtag ccagcgagtt tgtcaccatg gcctcccagc
     6181 tggtctatat caagacgagg atgctcctct ccatccacga cgaggaggcc ctgagcgaga
     6241 tggagcagct cattgccagc ctggaggagc accagcgcaa tgagaactat ctgaaaatca
     6301 aggagatcac ccccctgctg gaccggcggt acagctatgg gagagacttc atcaccaaag
     6361 tgccggagac cattcagccg gacaggacct accgctatgt ccacgacagg gaggacctga
     6421 aaaaggctat tctgtctgtc ttgtccaggg cggaccacca gctgccgccc ccagtggcgg
     6481 cttttcaagg catcgtgggg cgggagccat accctgtgtc ggaaaaggca ggggagatca
     6541 tccgccggct gctccggacc ggggtcaccc ggttccgcgc cctgttccag ggtaaccgca
     6601 gccgctccga ggtggtggcc accttcctgg cggtgctgga gctgtgcaag gcccaccgcc
     6661 tgcggctggc cgggacggag gaggactgta ccatcacctc cacccagggg gagcaggggg
     6721 accatttaga ggtcacagcg gattcctttg aattttaaca ggatgggaga agaggcatgg
     6781 aactgaagga gctggaggcc gcgctggagg gggtcctgtt cgccgccggg gagccggtgg
     6841 gggtggagcg cctgtgtctg gggctggagg tggatcgggc caccatggac gcagtggccc
     6901 agcggctgat ggaccggtac agctatgagc ggcggggtat ccgcttggtg cggctggata
     6961 ccagctacca gctgtgctct gctccggaat tcgctcccta tatccgcaag actttggaga
     7021 atcggaagcc ggcgcggctg tcccagcctg cgctggaggt gctggcggtg atcgcctact
     7081 accagccggt gacccgggcc tatgtggacc agatccgggg ggtggacagc gcctatacgg
     7141 tgggactgct gctggagcgg gagctcattg aggagtgcgg tcggctggcc gttcccgggc
     7201 ggcccatcct gtaccgcaca acgaaaaatt tcctgcgctc ctttgggctg tccagcctgg
     7261 aagagctgcc tgagctgccg gatgcctccc aggagagcgg acagctgacc ctggagctgg
     7321 aggcggcggt agcccgtttg agggcggagg agagtgggca gactgtccaa gaggatgggg
     7381 cagctcaccc ggcccaggag gagacggaat gagggggtgg atcgttctgg cggcggtcct
     7441 gctgctgttg ttcctgctgg ggcagctgcg gctgggcgcg gtgggggagt atggtcccgg
     7501 aggcgccgga gcctggttcc gggcggggcc tgtccgcatc cgcctctacc caaggaggcg
     7561 ctcagggcct tcgaaaaagg agaaggcgcc caagcccggg aaggagcacc ctgcggggga
     7621 ggcacgcggc ggcggacttt tggagctgat gaaggagctg ctgcccctgg ccttggaggc
     7681 ggcgggctgt ttccgccgca ggctgcgggt ggatcagctg gagctgaagc tcaccatcgg
     7741 cgcggcggac ccaggagacg cagctatccg atatggacag gccaacgccc tgctgggaag
     7801 catctggcag cccctgacag aggcgttcca tgtggaggag ggccgggctc gggtggaggt
     7861 ggacttcacc gcggcggccc cggtgctgac ggggcaggtg tccctgtccc tgaagctgta
     7921 tcagctccta tggctggggc tgcacttcgg cctgcgggcc ctgagtacct atgtgaacca
     7981 tcgaaatcgg cataaacagc agagagaggc ggtttgaata tggaaaaaca gcatcccatc
     8041 aatgaaatga tgagcactac aatggagaag atccgcagca tgatggacgc caacaccatc
     8101 attggtgacc ccatccagac cggggaggtc accctgatcc cggtctccag gctgtccttt
     8161 ggatttgcca gcggcgggag cgactttgtg accaaaaatc agaaacccgg ggctgagaac
     8221 acctttggcg ggggcagcgg cgccagcgcg aagatggagc cggtggcctt cctcatcatc
     8281 aagggggaca gcgtcaagct gctcccggtc gatccacctc ccgccaccac ggtggaccgg
     8341 gtcatcgagg tggtgcctga ggtggtggaa aaggtcaccg atttcctgga aaagcagaaa
     8401 aaagcagacg agcccccgga gggaacggac cactttgtgg tgtgaccgca ctgatctgac
     8461 agaatttgcc ggatccggca gggagagaaa accggcggcc cggaaaatac taaggtcact
     8521 ttgtttgatg gaaggtgacc atattgaaac gactgctggc ggcggcgctg gccgctggat
     8581 ctatgctctg ccaggtgccc actgcccagg cggtgggcac ctccgcgtcc tctgccatcc
     8641 tgatggaggc agagagcggc cgggtgctct atgaacaaaa cgcccacgag gaacggctca
     8701 tcgccagtat caccaagctg atgaccgccc tggtggcgct ggagtccggc cacgacctgg
     8761 aggagaccgt cacagtgggg gaggcctgca cccgggcaga gggttcctcc ctctatctgc
     8821 gcccagggga ggagatctcg ctgaaggggc tgctctacgg ggtgatgctg cgctccggga
     8881 acgacgcagc gctggcagtg gcggagcact gcgggggcag cgtggaggag ttcgttgggc
     8941 ggatgaatga aaaggcggcc cagttgggga tggtgaacag ccatttcgcc aaccccaacg
     9001 ggttgaacgc ggaggggcat tactcctctg cctacgacat ggccctgctg gcccgggcct
     9061 gtctggagaa cgaggcactg gcggagatcg ccgccaccaa gagcgccact gtggacggcc
     9121 gggtgctgac taaccacaac aagctgctgt ggcagtacga gggctgcatc ggcctgaaaa
     9181 cgggctatac agaaaaggcg gggcggaccc tggtgtccgc ggcccggcgg ggtgggatga
     9241 cgctgatcgc ggtgaccctc aacgacccgg atgactggcg ggaccatacc gccctgctgg
     9301 actacggatt ttccacctat gccccggtga cgctgacaga ggagggaaag gccgtggcac
     9361 ggcggcccgt gtccggcagc ctggtcccct ttgtggagat ccaggcggca gagacggtgc
     9421 ggatcccggt gtgtgaagag gacaaaatcg actgggaact ccagtgggac caggaggcgc
     9481 tggaggctcc tctccgggcc ggcggcagtg cgggccggat ggtctgccgg gtgaacggcg
     9541 aggaggcggc ctgcgtgcct ctggtgttcg cccaggatgt ggaccgtgcg ctccagcctc
     9601 ctggtgggat ctggactcgg cttatggagc tttggaacag ataaagaaag agagggggga
     9661 gtcagcatgg aggagcgcct gcaaaagctg ctgtcagcct gcgggctggc ctcccgccga
     9721 acggtggagc agtggatcga ggcggggcgg gtcaccgtca atgggacgaa ggcacggctg
     9781 ggggacaagg cggacctgga ccgggaccgc atcgaggtgg atggccggcc ggtccggccc
     9841 ggcggagacc ggctgtatct gatgctgaac aagcctcgag ggtatgtgac caccctgtct
     9901 gacgagaagg ggagaaagac ggtggcggag ctggtctccg gatgcggacg gagagtgtgg
     9961 ccggtggggc ggctggactt ggactccgag gggctgctcc tgctgaccga cgacggggaa
    10021 ctgacccaga agctgctcca ccccagtcat caggtggaaa aagaatatct ggtgtgggtc
    10081 actggggacg tgagccgggg gctgccggtg ctgtccggcc ccatggatct ggacggcgtg
    10141 ccgctgtccc cggccaaggt gcggcggggg aggaccagcg gaggagtgac ccagctatcc
    10201 atcaccatct gtcaggggaa aaaccggcag gtccggcgca tgtgcggtca ggcggggctg
    10261 aaggtgctgc gcctgaaacg ggtacgggag ggagggctct gcctggaccg gaccctgggg
    10321 cccggaaagt ggcggagact ggagcagagc gaggtggcct ccctgctgca aaagccctga
    10381 ttttttgccc cgtaccggat agaacataaa ttttttgcaa gataaagttt ttatcctgca
    10441 aaaatggctt gcttttttcc gggaaacaag gtatcataag gagctgaaac agctggtttc
    10501 ctcaaagaga aaagcaggag ttgaccgaga tgaatcgaga cattttgact gtaatccagg
    10561 aaaatatgag caccttctcc aaagggcaga agcggatcgc caactatatc ctggaatctt
    10621 atgacaaggc ggcctttatg accgccagcc gcctgggcaa gaccgtcagc gtcagcgagt
    10681 ccaccgtggt ccgttttgcg gcggagctgg gctatga
//
LOCUS       DBMDSJ010000008        10577 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2088_length_10577_cov_1.687476, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000008 DBMDSJ010000000
VERSION     DBMDSJ010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10577
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2088_length_10577_cov_1.687476"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="ACZPAQ_00525"
     CDS             complement(<1..360)
                     /locus_tag="ACZPAQ_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR0902487.1"
                     /translation="MEREQLSWQERGQLWLRLIIRLVLAAAAVVLVAKLGPPLLSLFA
                     PFVAALIVAALLNPAVRWLQRRLGWSRKLLSLLLVVVIFGVIGGCLGILTYAAANELV
                     SLAQNWSGMLTQVEGFFF"
     gene            complement(556..1422)
                     /locus_tag="ACZPAQ_00530"
     CDS             complement(556..1422)
                     /locus_tag="ACZPAQ_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR0902488.1"
                     /translation="MAKILFLTDSAADIPKSLREELGIRVMPFPIAMGERELRDGEDF
                     TPQEFYQALAEEPKIPTHAQLTAYQFQQCYEETWKAGYTDLIYTSINSEGSATYQNAV
                     QARGEFFEDHPEARDAFSIRVIDSHTYTMCYGYPVAEGARMVAAGASVEEIAAYIQDW
                     VDHVRVLFVPFDLKFAKKSGRVSAAAAFVGEALGLKPIMTFENGASKVVTKVRGEKNV
                     IPTILELAQKEREPGTPYLIIRAARPDQAELLTESCRQAFGQEPAMDCFIGGVIAINA
                     GPNLVGLIYRKK"
     gene            complement(1727..>1952)
                     /locus_tag="ACZPAQ_00535"
     CDS             complement(1727..>1952)
                     /locus_tag="ACZPAQ_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR0902489.1"
                     /translation="DNIDGVLELPKGRCVFTYHTGDYLSIGRSYERILEYCRTHHFNI
                     VSDSYEFAINDYLSTADESEYITKILFYIA"
     assembly_gap    1953..1962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1963..2534)
                     /locus_tag="ACZPAQ_00540"
     CDS             complement(<1963..2534)
                     /locus_tag="ACZPAQ_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024803.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR0902490.1"
                     /translation="MKNLFSIGELSKLQNISRQTLIFYDKIGLFRPAYTDPDNGYRYY
                     SSSQLDYLDTICIMKRIGFSLNEIKAHMKSFRIENSIAALRKQLSTINHQIEELQMIK
                     NRVEHRCLQLEHSISIRDCSDIVSIEEVSPQYILLQKVTEPYTLEMLSIATKECFVRS
                     SKEQLPIFFQSGAIVPYERILRGRYTEASFA"
     gene            2616..3254
                     /locus_tag="ACZPAQ_00545"
     CDS             2616..3254
                     /locus_tag="ACZPAQ_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0902491.1"
                     /translation="MIPRMTAICKKLADGLTFKKILLILLGAMICTFGIHNIHQRTNI
                     TEGGVIGLMLLVEHWLKISPAYITPMLDAACYLLAFKFLGGNFIKISIISTLFVSVFY
                     KIWELLPPMLPDLSAHPLIAAVLGGIFVGVGVGLIVRQGGSSGGDDALALTISHVSHW
                     KLSRSYLFTDTVVLLLSLTYIPIMRIVFSLITVTVSSNLIDWIKEYKFKTIR"
     gene            3679..4476
                     /locus_tag="ACZPAQ_00550"
     CDS             3679..4476
                     /locus_tag="ACZPAQ_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH family RNA-binding protein"
                     /protein_id="HIR0902492.1"
                     /translation="MNKTELLNKCARSGEERVLLARALDKLELAQNRSVPACTPFLSP
                     GEQASVTDLLNAWGRPRHLFFGGYEGAERAVCAFLPDWQEESDFLADPEGPVAALEAA
                     FPADAELSHRDILGALMGLGITREKLGDILLDHGRCQVLVLRDALPILLSQWESAGRW
                     KMKLREIPLEQLEAKPPQVKTIRDTVAALRLDAVLAAGFSTSRSKAADLVSAGRVSVN
                     HRECAKADRTVGEGDVLSCRGLGKCVVKEVLGQSKKGRFMLVLERYI"
     gene            4494..4700
                     /locus_tag="ACZPAQ_00555"
     CDS             4494..4700
                     /locus_tag="ACZPAQ_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR0902493.1"
                     /translation="MTQEKIDRINALARKAKTPEGLTAEETAERAALRQEYVAAVRAN
                     LEAQLDNTLIQEPDGTRHRLPKKP"
     gene            4697..5914
                     /locus_tag="ACZPAQ_00560"
     CDS             4697..5914
                     /locus_tag="ACZPAQ_00560"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR0902494.1"
                     /translation="MRRGVLWALLLSFLLAGCAPAGAPAGEGSGQEPSQSAMAPAPEA
                     GTSAQPQPAPEPEPPTQEELLQAMVDTMTPEEKAGQLFWVRFPETGWDELAREWHPGG
                     FLLFGRDFKNRTPEELRTLLASLQETSPIPLLLGVDEEGGTVVRASYYPAFRAEKFRS
                     PQALFAAGGLEAVRADALDKSAFLLDLGLNVNLAPVCDVSTDPADFIYARAFGQDAQA
                     TAEYADTVVSAMGEAGIGSVLKHFPGYGSNRDTHTGIAVDQRPLEQFRQSDFLPFQAG
                     IDAGAGGVLVSHNIVTCLDPELPASLSPASYQVLREELGFEGVAMTDDLDMDAVQKYV
                     EEGTAPVMALQAGADLVLTSDPQGGIPSVLEAVETGALTWDRLDQSVLRVLRWKLQLG
                     LLSLPGAEDVQTS"
     gene            6056..7315
                     /locus_tag="ACZPAQ_00565"
     CDS             6056..7315
                     /locus_tag="ACZPAQ_00565"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR0902495.1"
                     /translation="MKEIYVVNCCRTAVGSFGGSLKDTPAPELGAVVVKEALSRAGVE
                     PEQVDELMFGCILTAAQGQNPARQVGVKAGLPYSVPAYTVGMVCGSGMKSVIEGARAI
                     LAGDADIIVAGGTENMSAAPYALPDERWGARMGDKKVVDTMIRDGLWDAYNNYHMGTT
                     AENICDVWGITRQELDEFAAASQQKAEAAQKTGRFEDEIVPVTVKKKKELVEFKVDEF
                     PRPGVTAEGISKLKGAFPVGPEGVEDEIVHTFELTQIHEADTRKHVQRVTAANASGIN
                     DGAAAIVLASGEAVEKYGLKPMAKLVSWGQGGVDPKIMGVGPVPASRQAMSKAGLKIE
                     DIDLVEANEAFAAQSVAVARELGFDMSKVNVNGGAISIGHPVGASGARIIVTLLHEMQ
                     KRPEAKRGLATLCIGGGMGVATIFEKC"
     gene            7446..8510
                     /locus_tag="ACZPAQ_00570"
     CDS             7446..8510
                     /locus_tag="ACZPAQ_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR0902496.1"
                     /translation="MKRWSVLVTAVLSFSILAGCAGGGEGTYLFDRLAPASGGMPGEP
                     EPRSLILTHSSPAGSLTDQAAQLLKEKVEEASEGQLQIQIYPEDSLGNLDDGRWYFEN
                     GAVDMRIGAGPSDVSSIATWLPTLTGCTPDEVSQALRPGQPLWDLICQQAGDTLVLGV
                     LPSSSRVLTSSRPVEDLSQLDGLMVRVIPSSSLDRRFWEALGAQTVEIPIQDLYLALQ
                     QGLADAQENPIYLVRSYNFHQQQKYLIPINFKVYLETIYLNLEVCRSLTEEQQALLRQ
                     AAEDTCREMVEVTARYLEECQDIFQREGMEVRPVSEEERARAREKVCPLVEEYLLSLY
                     GADVYQQVLDALSAAPEQGS"
     gene            complement(8539..10455)
                     /locus_tag="ACZPAQ_00575"
     CDS             complement(8539..10455)
                     /locus_tag="ACZPAQ_00575"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021982.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1 family esterase"
                     /protein_id="HIR0902497.1"
                     /translation="MRSKSIRLPLAAAALSLAMLTSCGAPGGAGSSVSSASSSGSGAQ
                     SAPAAVQVEPLTVQDFPCSTTEEFTALFQKMVQETPDQIYYHPYTGLFQEAVEADGLS
                     QGRKLYTYIPEETGHCASSVFVALPSGEKAEEFLVSSGWTAVADQYKFLLHALTPADS
                     QWGGEEELDYLSAAFALGSKTIHYSPYTGNYYFVGYADGGRLLEQWVMANPDNCSGLA
                     VLDGGAIDEAYLIQMGQTPAVDPEKTVNEVNVPVWLIEKEITDGVQAVADYWKGANDC
                     TETAYINQDVPLETTVYQQNMLSHDTFINAYPLGKVQLTQAEVSYTDPELSRTLWETF
                     LCKAQRYRSLAGNDLRPAIDFEALGFTKEERTIDGYSRYWLEYVPQSVKDDPSQAVPL
                     VMALHGAGQCAEAYAPYSEWFKVAEEAGFIVVFPTAYPYAENNGMARPIHNDCWDPTR
                     PDDISFWRQLIQDVSERYSIDESRIYATGHSNGGNSSAMIAGEMSDVVAAVAISAGRY
                     RNVDQQVTEDVATLHPMASTNRVPVIQLVGTKDAGAYQSPSLTSTMMYWLERNGCEDL
                     NAPLMYQTSGYHNQIWCDGDGVPMVRFAVIEDKPHTTTPSESRLFWYDFLCHYSRGED
                     GSVQYMQDDTIIRN"
BASE COUNT         2022 a         3240 c         3197 g         2108 t
ORIGIN      
        1 aaagaaaaag ccctccacct gggtcagcat cccgctccaa ttctgcgcca gggagaccag
       61 ttcgttggcc gcggcatagg tcaggatgcc cagacagccg ccgatcactc cgaagatgac
      121 caccaccagc agcagggaca gcagcttccg gctccagccc agcctgcgct ggagccagcg
      181 caccgccggg ttgagcaggg ccgccacgat cagcgccgcc acgaaggggg cgaacaggga
      241 cagcagggga ggccccagct tggccaccag caccaccgcc gctgctgcca gcaccaggcg
      301 gatgatcagc cgcagccaca actgcccccg ctcctgccag gacagctgtt ctctctccac
      361 aacgaccact ccttcacagt atcacatcga taccttcagt tttccatagc atagtccagc
      421 ctatcacaaa aaggatgaat aatatgtaaa tcccgggaat ctcttccccg ctttctttcc
      481 tcctcccgcc ccgcccaagc cgggaggaca aaataaggcg cgccgcagga cccgggtccc
      541 gcggcgcggc cagttttatt tcttccggta gataagtccc accagattgg gtccggcgtt
      601 gatggcgata acgccgccaa tgaagcagtc catggccggc tcctgcccaa aggcctgacg
      661 gcaggactcg gtcagcagct ccgcctggtc gggccgggcg gcccggatga tcagataggg
      721 cgtccccggc tcccgctcct tctgcgccag ctccagaatg gtggggatca cgttcttctc
      781 cccccgcacc ttggtcacca ctttggaggc tccattctca aaggtcatga tgggcttgag
      841 ccccagggcc tctcccacaa aagccgcggc ggcggagacc ctcccagact ttttggcaaa
      901 cttcagatca aaggggacaa agagcacccg cacatgatcc acccagtcct ggatataggc
      961 ggcgatctcc tccacagagg cgcctgcggc aaccatccgc gccccctctg ccactgggta
     1021 gccgtagcac atggtatagg tgtggctgtc gatgacccgg atggagaagg catcccgggc
     1081 ctccgggtgg tcctcaaaga actccccccg ggcctggacg gcgttctggt aggtggcgct
     1141 gccctcggaa ttgatggatg tgtagatcag gtctgtgtac cctgctttcc aggtctcctc
     1201 atagcactgc tggaactggt acgccgtgag ctgggcatgt gtcgggatct tgggctcctc
     1261 cgccagcgcc tgatagaact cctgcggggt gaaatcctcc ccatcccgca gttcccgctc
     1321 ccccatggcg atggggaagg gcatgaccct gatccccagc tcttcccgca gggacttggg
     1381 gatatcggcg gcggaatcgg tcaaaaacag gatcttcgcc atagcggtct ccttccatga
     1441 tacgcgccgt gggcgcggac ctccaatttt acaccaatat tattgcagaa agccccccgc
     1501 ttgtcaactc cggcctcgtc cgccctccag ctcgctgacc tgggagttgc tgtataaagg
     1561 atggtttgca ccacctgcta cattggcaga acgaaacgac cgttggaaag gagtccgctg
     1621 gaaaacaagc agggacatga cacgccgatg ggcggtcaag tcccctcttt cagctctatg
     1681 aattaagatc tattctttat tgactgctgt ttgaaaaagt atgctgttac gcaatataga
     1741 acaaaatttt cgttatatat tcgctctcgt cggccgtgga aaggtagtca ttgatggcga
     1801 actcatatga atcagaaaca atattgaagt gatgggttcg gcagtattct aaaatgcgtt
     1861 cgtaggatct tccgatggaa agatagtccc ctgtgtgata ggtaaatacg caccgccctt
     1921 tgggaagttc cagaacgccg tcgatattat cannnnnnnn nngcaaacga tgcttcggta
     1981 tatcttcccc gcagaatacg ttcatacggc acgatcgcac cgctctgaaa gaaaattgga
     2041 agttgctcct tggatgaccg aacaaaacat tcctttgtag caatgctgag catttccaac
     2101 gtataaggtt ccgtgacctt ttgaagcaaa atgtattggg ggcttacttc ctctatggaa
     2161 acgatatcgc tacaatcacg aatggaaata gaatgctcta actgcagaca ccgatgttca
     2221 acgcggtttt ttatcatttg cagctcctca atctgatgat tgatggtaga gagctgtttg
     2281 cgcagcgcag cgatagaatt ttcaatccta aaacttttca tgtgtgcttt aatctcattg
     2341 agggaaaagc cgattctttt catgatgcaa atcgtatcca aatagtcaag ctggctggaa
     2401 ctgtaatagc ggtaaccgtt atcaggatca gtatatgcag gacggaaaag tccgatttta
     2461 tcgtagaaaa tcaaagtctg gcgagaaatg ttttgcagtt tggaaagttc accgatggaa
     2521 aataaatttt tcatataagc cccttgacct tatagttact atatagttta ttattttagc
     2581 atgaaaatat gaacttatca aggaggctgc tcaaattgat accccgtatg actgccattt
     2641 gtaaaaagct tgcggatggc ctgactttca agaaaatact gctgatatta ctcggagcca
     2701 tgatctgcac ctttgggatc cacaacattc accagcggac gaacatcaca gaaggcggtg
     2761 tgattggctt aatgctttta gtcgagcatt ggctgaaaat ttctccggct tatattaccc
     2821 ctatgctgga tgctgcttgt tatttgctcg cattcaaatt cctgggtggg aactttatca
     2881 aaatctcaat tatttcaaca ctgtttgttt ctgtatttta taagatctgg gagctgcttc
     2941 cgccaatgct ccccgatttg tccgcacatc cgttgatcgc cgctgtgtta ggcggtattt
     3001 ttgtgggtgt aggcgtcggc ttaattgttc gtcagggagg ttccagtggt ggagatgacg
     3061 ccttggcttt aacaatttct cacgtctccc actggaagtt atcccgttct tatttgttca
     3121 cggatactgt agtgctgcta ttatccctta cctatattcc cataatgcgt attgttttct
     3181 cattgataac cgttactgtt tcttcgaact taatcgattg gataaaagaa tataaattta
     3241 aaaccatcag atgaatatcc aatcacaaaa agcgacagat actctctgtt tttccacctt
     3301 cgctttgaca gtaaaaacac cctcagcaat agaaagacca cacctcggtc tgctggcttg
     3361 tccagccgcc gggatgtggt ctttgtgatc tctgatcatt ttgcacgtct cctggttccg
     3421 cccttcttta tataaggccg tcatcagaga tctgcctccc tccgttattc cttattttga
     3481 ctgcgggagg ttttttatat cctacatcag ctcaaagagt aaaaaggagc cgcccgctgg
     3541 ggcatactgg cgggagggga cgcggcgggc ggcctgtctg cccttctttc cgcgccctgt
     3601 caagaccgga gcagcggccc ctcgttgaca ccaccggccg gatgggatat actacaccca
     3661 ccagaaaagg aggcctccat gaacaagacc gaactgttga acaaatgtgc ccgcagcggg
     3721 gaggagcggg tgctgctggc ccgcgccctg gataagctgg agctggccca gaaccgctct
     3781 gtccccgcct gcaccccatt tctctccccg ggagagcagg cctccgtcac cgatctgctc
     3841 aacgcctggg gccgcccccg ccacctgttt ttcggcggat atgagggggc ggagcgggcg
     3901 gtgtgcgcct ttctgcccga ctggcaggag gaatccgact ttctcgccga cccggagggg
     3961 ccggtggccg cgctggaggc cgcctttccc gcggacgcgg agctgtctca ccgagacatc
     4021 ctgggtgccc tgatgggact gggcatcacc cgggaaaagc tgggggacat cctgttagac
     4081 catggccgct gtcaggtgct ggtcctgcgg gatgccctgc ccatcctcct ctcccagtgg
     4141 gagtctgccg gccgctggaa gatgaagctg cgggagatcc ccctggagca gctggaggcg
     4201 aagccgcccc aggtgaagac cattcgggat acggtggccg ccctgcggct ggacgcggtg
     4261 cttgccgccg gcttctccac ctcccggtcc aaggcggcgg acctggtctc cgccggccgg
     4321 gtgtcagtaa accaccggga gtgcgccaag gcggaccgga cggtggggga gggcgacgtg
     4381 ctctcctgcc ggggtctggg caagtgcgtg gtgaaagagg tgctgggcca gtctaaaaag
     4441 ggccgcttca tgctggttct ggaacggtat atctgagaag gagatacgct atgatcacac
     4501 aggaaaaaat cgaccgcatc aacgccctgg cccggaaggc caagactccg gagggcctga
     4561 cggcggagga gacagcagag cgcgccgccc tccgtcagga gtatgtggcc gctgtgcggg
     4621 ctaatctgga ggcccagctg gacaacaccc tcatccagga gccggacggc acccgccacc
     4681 ggctccccaa aaagccgtga ggcggggggt cctttgggcg ctgctcctgt cctttctgct
     4741 ggccggctgc gcaccggcgg gggcgccagc aggggagggg agcggccagg agcccagcca
     4801 gtccgccatg gccccggcgc cggaggccgg cacttccgcc cagcctcagc ccgcgccgga
     4861 gcccgagccc cccacccagg aggagctgct ccaagccatg gtggacacca tgacgccgga
     4921 ggagaaagcg ggccagctgt tctgggtccg tttcccggag accggctggg atgagctggc
     4981 ccgggagtgg caccccgggg gctttctcct cttcggccgg gacttcaaaa accgcacccc
     5041 ggaggagctg cgcactctgc tcgcctccct ccaggagacc tcccccatcc ccctgctcct
     5101 gggggtagat gaggaggggg gcacggtggt ccgggccagc tactaccccg ccttccgggc
     5161 ggagaagttc cgctcccccc aggccctctt cgctgccgga gggctggagg ccgtccgggc
     5221 cgacgccctg gacaagtccg cctttctgct ggatctgggc cttaacgtaa acctggctcc
     5281 ggtgtgcgac gtgtccaccg accccgctga cttcatctat gcccgcgcct tcggccaaga
     5341 cgcccaggct acggcggagt atgcggacac ggtggtctcc gccatggggg aggcgggcat
     5401 cggctcggtg ctcaagcact tcccgggcta cggcagcaac cgggacaccc acaccggcat
     5461 tgcggtggac cagcgccccc tggagcagtt ccgccagtcc gacttcctgc ccttccaggc
     5521 aggcatcgac gccggggccg gcggtgtgct ggtctcccac aacatcgtca cctgcctgga
     5581 cccggagctg cccgcctccc tgtccccagc ctcctaccaa gtgctgcggg aggagctggg
     5641 ctttgaagga gtggccatga ccgacgacct ggacatggat gcggtacaga agtatgtgga
     5701 ggagggcacc gccccggtga tggccctcca ggccggggct gacctggtcc tcaccagcga
     5761 tccccagggg ggcatcccct cggtgctgga ggcggtggag accggggcgc tgacctggga
     5821 ccggctggat cagtcggtgc tgcgggtgct gcgctggaag ctccagctgg gactgctgtc
     5881 cctccccggg gcggaggatg tacaaacaag ctaagatttg ccgaaatttc cgcctcattt
     5941 cgcaagaaac ccctttgcgt tcccggaaaa gttcgctata atggagcatg aagatcccga
     6001 ggtcccggcc gccgctccgg cgcgcctgat gaaagaatgg aggtcaacga tcccaatgaa
     6061 agagatctat gttgtcaatt gctgccgcac cgccgtgggc tccttcggcg gaagcctgaa
     6121 ggacaccccc gcccccgagc tgggtgccgt ggtggtgaag gaggccctgt cccgggccgg
     6181 cgtggagccg gagcaggtgg acgagctgat gttcggctgc atcctcactg ctgcccaggg
     6241 ccagaacccc gcccgccagg tgggcgtcaa ggccggcctg ccctactccg tccccgccta
     6301 caccgtgggc atggtgtgcg gctcgggcat gaagtcggtc atcgagggtg cccgggccat
     6361 cctggccggg gacgccgaca tcatcgtggc cggcggcacc gagaatatgt ccgccgcccc
     6421 ctacgccctg cctgacgagc gctggggcgc ccgcatgggg gacaagaagg tggtggacac
     6481 catgatccgg gacggactgt gggacgccta caacaactac cacatgggca ctactgctga
     6541 gaacatctgc gacgtgtggg gcatcacccg ccaggagctg gacgagttcg ccgccgcctc
     6601 ccagcagaag gccgaggccg ctcagaagac cggccgcttc gaggacgaga tcgtccccgt
     6661 cacggtgaag aagaagaaag agctggtgga gttcaaggtg gacgagttcc cccgccccgg
     6721 cgtcaccgcc gagggcatct ccaagctgaa gggcgctttc cctgtgggcc ccgagggagt
     6781 ggaggacgag atcgtccata cctttgagct cacccagatc cacgaggccg atacccgcaa
     6841 gcacgtccag cgggtcaccg ccgccaacgc ctccggcatc aacgacggcg ccgccgccat
     6901 cgtgctcgcc tccggcgagg cggtggaaaa gtacggcctg aagcccatgg ccaagctggt
     6961 cagctggggc cagggcggcg tggaccccaa gatcatgggc gtgggcccgg tgcccgcctc
     7021 ccgccaggcc atgagcaagg ccggcctgaa gatcgaggac atcgacctgg tggaggccaa
     7081 tgaggccttt gccgcccagt ccgtggccgt ggcccgggag ctgggcttcg acatgagcaa
     7141 ggtgaatgtc aacggcggcg ccatctccat cggccacccc gtgggggcct ccggtgcccg
     7201 catcatcgtc actctgctcc acgagatgca gaagcggccc gaggccaagc gcggcctggc
     7261 caccctgtgc atcggcggcg gcatgggtgt cgccaccatc tttgagaagt gctgagccgg
     7321 acaccggcgt ccccgggggc tgacctcccg gggcccggtc cggcaggcgg gggcggcggg
     7381 cttgtgcccg ccgccctttt cccggaattt tcgaccgaat gggccggcag gggaggggga
     7441 gaggtatgaa acgctggtcc gtcttagtga cggctgtact gagtttttcc atactggccg
     7501 gctgtgccgg cgggggagag gggacctatc tctttgaccg cctcgccccc gcatccggcg
     7561 gaatgcccgg agagccagag ccccgcagtc tgatcctgac ccactcctcg cctgcgggca
     7621 gcctcaccga tcaggctgcc cagctcctga aggagaaggt ggaggaggcc tccgagggcc
     7681 agctccagat ccagatctac cctgaagact ccttgggcaa tttggacgac gggcggtggt
     7741 actttgagaa cggggcggtg gacatgcgca tcggggccgg ccccagcgat gtctcctcca
     7801 tcgccacctg gctccccact ctcaccggct gcactcccga tgaggtgagc caggctctgc
     7861 gcccaggtca gcccctgtgg gatctgatct gccagcaggc gggggacacc ctcgtcctgg
     7921 gggtgctgcc atcctccagc cgggtgctca cctccagccg gccagtggag gacctgagcc
     7981 agctggacgg cctgatggtc cgcgtcatcc cctcttcctc cctggaccgg cggttttggg
     8041 aggctctggg ggcgcagacg gtagagatcc ccatccagga cctctacctg gccctccagc
     8101 aggggctggc agatgcccag gagaacccca tctatttggt gcgcagctac aacttccacc
     8161 agcagcagaa atacctgatc cccatcaact ttaaggtata tctggagacc atttatctca
     8221 atctggaggt gtgccgcagc ctgacggagg agcagcaggc cctgctccgg caggccgcgg
     8281 aggatacctg ccgggagatg gtggaggtaa ctgcccgcta tctggaggag tgccaggata
     8341 tctttcagcg ggagggcatg gaggtccgcc ctgtgtcaga ggaggagcgg gcccgcgccc
     8401 gggagaaggt ctgcccgctg gtggaggaat atctgctgtc cctgtatggc gctgatgtct
     8461 atcagcaggt gctggacgcc ctttccgccg ccccggagca gggaagctga gggtgtccgc
     8521 atgtacgatc tccttctttt agttgcggat aatggtgtcg tcctgcatat actgtacgct
     8581 gccgtcctcg cctctggagt agtggcagag gaagtcatac cagaagagcc gggactcgga
     8641 gggggtggtg gtgtggggct tgtcctcgat cacggcgaag cggaccatgg gcacgccgtc
     8701 cccgtcgcac cagatctggt tatggtatcc gctggtctga tacatcaggg gggcgttcag
     8761 gtcctcacag ccgttccgct ccagccagta catcatggta gaggtcaggc tggggctctg
     8821 gtaggcgccg gcatccttgg tgcccaccag ctggatcacg gggacccggt tggtgctggc
     8881 catagggtgg agggtggcca catcctcagt cacctgctgg tccacattcc gatagcggcc
     8941 ggcactgatg gccacggcgg ccaccacatc ggacatctca ccggcgatca tggcgctgga
     9001 gttgcctccg ttggaatgcc cggtggcata gatccggctc tcgtcgatgg aatagcgctc
     9061 cgacacatcc tggatcagct gccgccagaa ggagatgtcg tcaggccggg tggggtccca
     9121 gcagtcgttg tggatgggcc gggccatgcc gttgttctcc gcataggggt atgctgtggg
     9181 gaacaccacg atgaagcccg cctcctcggc caccttgaac cactcagaat agggggcgta
     9241 ggcctcggcg cactgtcctg cgccgtggag ggccatcacc agcggcaccg cctggctggg
     9301 gtcatccttg acggactggg gcacatactc cagccagtaa cgggagtaac cgtcgatggt
     9361 ccgctcctcc ttggtaaagc ccagcgcctc aaagtcaatg gcggggcgca ggtcgtttcc
     9421 ggccagggag cggtagcgct gggccttgca caggaaggtc tcccacaggg tcctgctcag
     9481 ctccgggtcg gtgtaactca cctctgcctg ggtgagctgt accttcccca ggggataggc
     9541 attgataaag gtgtcatggg aaagcatgtt ctgctgatac accgtggtct ccaggggaac
     9601 gtcctggttg atataggccg tctcagtgca gtcgttggct cccttccagt aatcggccac
     9661 ggcctggacc ccgtcggtta tctccttctc gatcagccac accggcacat tgacctcatt
     9721 cacggtcttt tccgggtcca cggcgggggt ctggcccatc tggatcagat aagcctcgtc
     9781 gatggctccg ccgtccagca ctgccagccc ggagcagttg tccggattgg ccatgaccca
     9841 ctgctccagc aggcggcctc cgtcggcgta gcccacaaag tagtagtttc cggtgtatgg
     9901 gctgtaatgg atcgtcttgc tccccagggc aaaggcggcg ctcagatagt ccagctcctc
     9961 ctcgcccccc cactggctgt cggcaggggt cagggcgtgg agcaggaatt tatactggtc
    10021 ggccaccgcg gtccagccgc tgctcaccag gaactcctcc gccttctccc cggagggcag
    10081 ggccacgaac acgctggagg cgcagtgccc cgtctcctcc gggatatagg tgtagagctt
    10141 ccggccctga gagagcccgt ccgcctccac cgcctcctgg aacaggccgg tgtaggggtg
    10201 atagtagatc tggtccggtg tctcctgcac catcttctgg aacagcgccg taaactcctc
    10261 tgtggtggaa caggggaagt cctgaacggt caggggctcc acctgaacgg ccgcaggggc
    10321 agactgtgcc ccgctcccgg agctggatgc cgaagagacg ctggagcctg cccctccggg
    10381 cgcaccgcag gatgtgagca tggccagaga cagtgccgcc gcagccagcg gcagacggat
    10441 agacttgctg cgcatgatgt gatcctcctt ttgcagattc ccgcaccggg agctgttctg
    10501 tattttgctc ccattctgtg ctgttttcag cttaacaggg ggcggaatac ctgtccatcc
    10561 tctgatttct ctgctgt
//
LOCUS       DBMDSJ010000009        10242 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2159_length_10242_cov_1.927423, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000009 DBMDSJ010000000
VERSION     DBMDSJ010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_10242_cov_1.927423"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            145..1701
                     /locus_tag="ACZPAQ_00580"
     CDS             145..1701
                     /locus_tag="ACZPAQ_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR0902498.1"
                     /translation="MGEKTKLQEMLEKKEKIRQGGGEKKVAKQHGAGKLTARERIDLL
                     FDPGTFQEYNIFMKHRCHDFGMEKVDTPAEGVVTGYGLIHGRGAFAFAHDFTVLGGAM
                     GEMQGMKVKRIQELAMDAGVPLIGLNDSGGGRIQEGPATSYGAIFFNNVMASGVIPQI
                     SAIMGPCAGGTVYSPALTDFIFSVDKTSRSFITGPQVIKNVTGEVVDAEKLGGARTHN
                     TVSGVSHFFCADDRDCIEKIKTLLSYLPPNCREMPPIMPCGDSPDRRCEMLNTFIPDN
                     LKKAYDIKDVIREIADGHEFFETQAMYATNMVTGFIRMNGRSVGVVANQPKAMAGCID
                     INASDKAARFIRTCDCFNVPLLSIVDVPGYMPGTNQEYGGIIRHGAKMIYAWAEATVP
                     KIVMAVGKVVGGARPAMCSWELRPDFIFAWPTAQMVVVGAEGAVDICRKHELKRAEES
                     GEDVAALRAHYIEEYKEEFFNPYKAAEYGKFEDVIEPAESRRVIIRTLELFQNKQVVL
                     PAKKHGNMPV"
     gene            1731..2930
                     /locus_tag="ACZPAQ_00585"
     CDS             1731..2930
                     /locus_tag="ACZPAQ_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR0902499.1"
                     /translation="MAVGYGPLSGVTVVDLTQFLSGPLATMIMADLGADVIKVERPDR
                     PKASGPFLNGERVYDLSVQRSKKSITLNLKAETDKKVLLELVKKADVLVENFKPGTME
                     RMGLGYDIIAAANPRIIYMAVSGFGYTGPYRSRGALDMIVQGMSGLMSLTGEPDGPAM
                     RCGTSASDVFTGLYAFGAISAALYDREKTGKGQFIDVAMLDATFSCLENAVINTCVFG
                     TDPQRVGNSHPTSVPFGTFPTSDGEIIITCSRDPAFYSLCRALGREDMVEDPRFSKAE
                     ARRQHKAELTEEISRFTRARTLDECERIFEAHGVPNGRINTMKMICADPQIAARNMIV
                     EVEHPVAGTYRMAGSPLKFGNYPDTTYEPAPTLGQHTREVLAEYLKMPEEEIETMLKE
                     QKELVHT"
     gene            3112..3612
                     /locus_tag="ACZPAQ_00590"
     CDS             3112..3612
                     /locus_tag="ACZPAQ_00590"
                     /inference="COORDINATES: protein motif:HMM:NF018979.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR0902500.1"
                     /translation="MKIKAKTGDLALGIFSILGGIIILILTKVQGLQLIKSGQMGPGF
                     FPTICGIAIAICGAMVLLEAARRIARAKTAQAEKEELETNILNLTELKNLLLFMLLGT
                     GVLLLSDYLGLLTCLGLSVIAYLKIWGREKWIKSIVIGVCMVAFLYGVFVLFLHVPVP
                     KGPLGF"
     gene            3628..5130
                     /locus_tag="ACZPAQ_00595"
     CDS             3628..5130
                     /locus_tag="ACZPAQ_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR0902501.1"
                     /translation="MDLLHNLALGFSVLADPMTIVYSLIGVVCGVIIGALPGLGPSVG
                     IAVLLPMCYGLDPACALCMLCGIYYGAMYGGSITSILINTPGDSAAIMTTLDGYPMAR
                     QGKAGKALGMSCLASVIGGTISVVLFMSLAPVVSNFATKFGPSEYFALMVLGLTSIAG
                     MTGKSPAKGFLAAFIGLFVAGIGIDLIFGTTKFVFGNVNLYSGIDFVPVAMGLFGIAE
                     IVCNYSDNNLKVDKGSLKVKNLLPSRHEWKLSLPHIGRGTILGFIVGMLPGAGATIAS
                     FLSYDMAKRTSKRGEEFGTGVIEGVAAPECANNAASMGAMVPMLTLGVPGSGATAIMM
                     GALMMLGISPGPSFFVNEADVAWGLIASMYLGNVILVCIGLGALSLFVRILNVKTSTL
                     NALIFAFILVGAYALENSMFTVGVTLFFGLLGYILKKVGVPAAPMVLALVLGAITETN
                     LRQALIIDNNNFGAVISHPISAVILVLAVLLAFGTPIKSLITKLWAKKKA"
     gene            5405..6238
                     /locus_tag="ACZPAQ_00600"
     CDS             5405..6238
                     /locus_tag="ACZPAQ_00600"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR0902502.1"
                     /translation="MVRKILEIVEKYDMCPVNIVPVNKEGGSGVVGYTYLTSKGAGYE
                     YALASTSASFYTQPLTGNSPFTPDESTFSFVAHMVKDPVVLVAHPSYGFTCIQDVVDY
                     AKEHPGELKWGGVGNASDDAIIMYMLNNIAGIDLTYVPYDDGGMCQAALLGGHIGLST
                     VSLAEAEEHMAAGNMVALAACADERFDAIPEVPTAAEQGFDIGHQQSRGIVMNAGVPE
                     EVLAYYSDLFQRVSETEDWIEYAESNAMTVSFMPYQEFAAYAAEMLEDYTTYLGMIPQ
                     D"
     gene            6400..6870
                     /gene="tadA"
                     /locus_tag="ACZPAQ_00605"
     CDS             6400..6870
                     /gene="tadA"
                     /locus_tag="ACZPAQ_00605"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR0902503.1"
                     /translation="MDHEAYMRRALELAAQAAEQGDVPVGCVIVRDGEIVGEGRNRRE
                     ENGDATAHAELEAIRDACRRLGSWRLHRCTMYVTLEPCPMCAGGIINARIGTVRYGAK
                     DDKAGACGSVLNLFEERFNHRPRLYRGPMEAECGRALRAFFEDLREKDGFSEEI"
     gene            7249..>8178
                     /locus_tag="ACZPAQ_00610"
     CDS             7249..>8178
                     /locus_tag="ACZPAQ_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR0902504.1"
                     /translation="MAEQKQDGPHPRREPRFPGPLTLEGVEQIFEGCSDFAERTALLH
                     NDPQRQVTLCYINGMVRGERINDYLLRPLAQDPRLSAVSEAEAFQLLGQGALYNLYVE
                     RRTTLDQAAVDLTMGWCVLFFPGRMDALSFFVGTEEKRAVGQSENEPALKGARDSFVE
                     SILTNTSLVRRRLRAPELKVRQHVVGRQSLTPAMVVYLDGIADPDTVKRVEEKLDAID
                     IDGVESAGNLEEYLTDASRTPFPLMPFTQRPDRFCAGLLEGRVGLLCDGLPFGYLLPG
                     TMDQFFRTSQDRAFNWMTASMLLLLRYFCMAATL"
     assembly_gap    8179..8188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8189..8756
                     /locus_tag="ACZPAQ_00615"
     CDS             <8189..8756
                     /locus_tag="ACZPAQ_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR0902505.1"
                     /translation="GLYIALVTFHPEAIPVKLALSIVAAKQEVPFSTVFEVLIMLVAF
                     EVLQEAGLRLPSPIGATVSILGGLVVGNAAVEAHIVSPAVLIAVAIAGVAGYTMPSQD
                     MAAALRLWRFLLAILASLGGLFGLAMGCGALICHLAGLESFGVPYLAPFTSAAGQERG
                     SPDLIRLPLPWMKWRNGAARSANRRNQR"
     gene            8753..9841
                     /locus_tag="ACZPAQ_00620"
     CDS             8753..9841
                     /locus_tag="ACZPAQ_00620"
                     /inference="COORDINATES: protein motif:HMM:NF051774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HIR0902506.1"
                     /translation="MKKVLAAALCGAVMFLLPGCGGERLPYAREMGDMALMRTMGVDT
                     GEGGAEQLHVTVSSGRRARGLQGEAEPPLVLSAERESISSACLAMQGLSDSYVFYGHV
                     DQLLLGEELSRRGILETLQYFSQDQELGMGTQVWIVRGGTAAEAISAEQERGVESRLS
                     TIQTDSEMGMAGMTRTVGETLTSLMEDGSAYLPGLVPGEDGALLEAGYGVVKDGMLAG
                     WLTGEQARGLELAAGQVGADILELETRGGGAAVRVTAVSVSCVPEFSGERLTGLELAC
                     RLSLRAEESWGEPEPAELCAAAEEREAARISAALEQLKGWNADCLGLARRAGQTESAR
                     WNALRAQWPEVFPELEIQVTVQASLLAA"
     gene            9856..>10242
                     /locus_tag="ACZPAQ_00625"
     CDS             9856..>10242
                     /locus_tag="ACZPAQ_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HIR0902507.1"
                     /translation="MKQEKVSFRQLMALLWAGLLGPAAEWLPSMVTERAGPAGWLVPL
                     LALPVLLAAGWLAGSLSRGAGGLSQGLMDSFGPGAGRAVLTIYIIWMELLLALRLRLS
                     AQRMMAAGERDGALWFFLPVLVLMALW"
BASE COUNT         1991 a         2728 c         3427 g         2086 t
ORIGIN      
        1 cagatccata atttttatct tatactaata taaaaaatcg ataattgcaa tatgcgttaa
       61 aacagattaa cctaaagaaa gaagaaaaat atttgatttt tgttggattg tgaccaaagg
      121 cgcagcagag aggaggacct tgtcgtgggc gaaaaaacaa agcttcaaga gatgcttgag
      181 aaaaaggaaa agatcagaca gggaggggga gaaaaaaagg tcgcgaagca gcacggcgcg
      241 ggtaagctga cagcccggga gcgcatcgac ctgctgtttg atcccggtac tttccaggag
      301 tacaacattt ttatgaagca ccgctgccat gactttggga tggagaaggt agacacaccg
      361 gcggaagggg tggtgacggg atacggattg attcatggga gaggggcctt tgcctttgcc
      421 cacgacttta cggtcctggg cggcgccatg ggggagatgc agggcatgaa ggtcaagcgg
      481 attcaggagc tggccatgga cgccggcgtg ccgctgatcg gcctgaatga ctcggggggc
      541 gggcggatcc aggaggggcc tgccacttcc tatggggcca tcttcttcaa caacgtgatg
      601 gcttctgggg tgatccccca gatctcagcc atcatggggc cctgcgccgg cgggaccgtg
      661 tattcccccg cgctgaccga cttcattttt tccgtggaca agaccagccg ctcctttatc
      721 accggaccgc aggtcatcaa gaacgtgacc ggggaggtgg tggatgcgga gaagctggga
      781 ggagcgcgga cccataatac ggtgtcaggc gtgtctcact ttttctgtgc ggacgaccgg
      841 gactgcattg agaagatcaa gaccctgctg agctacctgc ccccaaactg ccgggagatg
      901 ccgcccatca tgccctgcgg cgactctccg gaccgccgct gtgagatgct caacaccttc
      961 attccggata acctgaaaaa ggcctacgat atcaaggatg ttatccggga gatcgcggat
     1021 gggcacgagt tttttgagac ccaggccatg tatgccacca atatggtcac cggatttatc
     1081 cgcatgaacg gccggtcggt gggggtggtg gccaaccagc ccaaggcgat ggcaggctgt
     1141 atcgacatca acgcttcgga caaggcggcc cgttttatcc gcacctgcga ctgcttcaat
     1201 gtgcccctgc tgtccattgt tgatgtgccc ggatacatgc ccggcaccaa tcaggagtac
     1261 ggagggatca tccggcacgg ggcaaagatg atctacgcct gggcagaggc cacagtcccg
     1321 aagatcgtga tggcggtggg aaaggtcgtg ggcggcgccc ggcctgcaat gtgctcctgg
     1381 gagcttcggc cggacttcat tttcgcctgg cccacggcgc agatggtagt cgtgggggca
     1441 gagggggctg tggacatctg ccggaagcac gagctgaagc gggctgagga gagcggtgag
     1501 gatgtggctg ccctccgtgc ccactacatc gaggagtaca aggaagaatt ttttaacccg
     1561 tataaggcgg cggaatatgg gaagtttgag gacgtgatcg agcctgcgga gtcacggcgg
     1621 gtcatcatcc ggactctgga gctgtttcag aacaaacagg tggtcctgcc agccaaaaaa
     1681 cacgggaata tgccggtctg acggcggcgc ggaaaggagc ttggttcaat atggcggttg
     1741 gatacggccc cctgagcggg gtaacagtgg tggacctgac gcagtttttg tccggtcccc
     1801 tggccacgat gatcatggcg gacctggggg cggatgtgat caaggtggag cggccggacc
     1861 ggcccaaggc cagcggcccg tttctcaacg gggagcgggt ctacgacctg tcggtccagc
     1921 gcagtaagaa gagcatcacc ctgaacctga aggcggagac agataagaag gtgctgctgg
     1981 agctggtgaa aaaagcggac gttctggtgg aaaatttcaa acccggcacg atggagcgca
     2041 tgggcctggg ttatgatatc attgccgcag ccaatcccag gatcatttat atggcggtct
     2101 ccggctttgg ctatacgggt ccataccgtt ccagaggcgc gctggatatg atcgttcagg
     2161 ggatgagcgg actgatgagc ctgaccggtg agccggatgg cccggccatg cgctgcggga
     2221 cctcggccag cgacgtgttt accgggctgt atgccttcgg tgccatctcg gcggccctgt
     2281 atgaccggga aaagaccgga aagggacagt ttatcgatgt ggcgatgctg gatgccacct
     2341 tctcctgcct ggaaaacgcg gtgatcaata cctgtgtctt tggcaccgat ccccagaggg
     2401 tgggcaacag ccaccccacc agcgtcccct tcgggacgtt ccccacctcc gatggagaga
     2461 tcattatcac ctgctcccgg gatcctgcat tttacagtct gtgccgcgcc ctgggacggg
     2521 aggatatggt ggaggacccg cgcttctcca aggcggaggc cagacgccag cacaaggcgg
     2581 agctgactga ggagatctcc agatttaccc gggcccgcac actggatgaa tgcgagcgga
     2641 tctttgaggc tcacggggtg cccaatggcc gcatcaacac catgaagatg atctgtgcgg
     2701 accctcagat cgcggcccgc aatatgattg tagaggtgga gcacccggtg gctggaacgt
     2761 atcggatggc gggcagcccg ctgaaattcg gaaattaccc ggataccacc tatgagcccg
     2821 cccccaccct gggacagcat acccgggagg tcctggcgga gtacttaaaa atgccggagg
     2881 aggagatcga aaccatgctg aaggagcaga aggagctggt gcatacataa gatggcgggc
     2941 ggagcgcggg acaggggacc ggcctgtgga atggaagatt ttgttttgtg cctgacgtgc
     3001 ggaatctggt ataatccgat gcgtattttg tggataaaag caaaaggagg tatcgagctc
     3061 tgagctgtgc ggaccagcaa agatcataga taagactaga gggaggatac tatgaagata
     3121 aaagccaaaa ccggagatct cgcactgggg atcttttcga ttctgggcgg aatcatcatc
     3181 ctgatcctga caaaggtgca gggacttcag ctgataaaga gcggacagat ggggcccggt
     3241 tttttcccca caatctgcgg gatcgccatc gcgatctgcg gggccatggt ccttctggag
     3301 gcggcgcgcc ggatcgcccg cgccaaaacg gcccaggcgg agaaggaaga actggagaca
     3361 aatatcctga acctgacaga gctgaagaat ctgttgcttt ttatgctgct ggggactggg
     3421 gtactgctgc tctccgacta tctggggctg ctgacctgcc tgggactgag tgtgatcgcc
     3481 tatttgaaaa tatggggccg agaaaagtgg atcaagtcga tcgtgatcgg tgtatgtatg
     3541 gtggcgttcc tgtacggggt ctttgtcctg ttcctgcatg tgccggtccc caaaggtccg
     3601 ctgggctttt aaggaggagg tacacatatg gacttgctgc ataaccttgc gctgggcttc
     3661 tcagtcctgg ctgatcccat gaccattgtg tactccctga tcggcgtagt gtgcggtgtt
     3721 atcatcggtg cgctgcccgg tctggggcct tcggtgggca tcgcagtgct cctgcccatg
     3781 tgctacggac ttgatccggc ctgtgccctg tgcatgctgt gcgggatcta ctacggggcc
     3841 atgtacggtg gttccattac ctccatcctg atcaatacgc ccggtgactc tgccgccatt
     3901 atgacgaccc tggacggata tcccatggcg cggcagggaa aagcagggaa ggccctgggc
     3961 atgtcctgtc tggcatcggt catcggcggg acgatatctg tggtgttgtt catgtctctg
     4021 gcacctgtgg tatccaattt tgctacaaag ttcgggccgt ccgaatattt cgccctgatg
     4081 gtgctggggc ttacctctat tgcgggcatg acagggaaat ctcctgcaaa gggttttttg
     4141 gctgcgttta tcggcctgtt tgtggcgggg atcgggattg acctgatctt tggtactaca
     4201 aagttcgtat tcggcaatgt aaacctctac tccggcattg actttgtgcc ggtggccatg
     4261 ggcttgtttg ggatcgcaga gatcgtatgc aactattctg acaataatct taaagtggat
     4321 aaggggagcc tgaaggtcaa aaatctgctg cccagcagac atgagtggaa gctgtccctg
     4381 ccccacatcg gccgggggac gattttaggc tttattgtgg ggatgctgcc aggggcgggt
     4441 gctacgatcg cttcttttct ctcctacgac atggcaaagc gcacctcaaa gcggggagag
     4501 gagttcggca ccggtgtgat cgagggtgtg gcggccccgg agtgtgccaa caacgctgcc
     4561 tccatggggg ccatggttcc tatgctgacc ttgggcgtcc ccggttcggg cgcgacggcc
     4621 atcatgatgg gtgcgctgat gatgctgggc atctcacctg gtccctcctt ctttgtgaat
     4681 gaggctgacg ttgcctgggg actgatcgcc tccatgtatc tgggaaatgt cattctggtg
     4741 tgcattggtc tcggcgcgct ctccctgttc gtgcgcatcc tgaatgtaaa gacctccacc
     4801 ctgaacgcgc tgatctttgc attcattctg gtgggcgcct atgcgctgga gaactccatg
     4861 ttcactgtgg gagtgactct tttcttcggc ctgctgggtt atatcctgaa aaaggtgggg
     4921 gtccctgcgg cacccatggt gctggcgctg gtgctgggtg cgatcactga gaccaatttg
     4981 agacaggcgc tgatcattga caacaacaat tttggagcgg tcatctccca tcccatttcc
     5041 gcggtcatcc tggtgctggc ggtcctgctg gcgttcggca cccccatcaa atccctgatc
     5101 acaaaactgt gggcaaaaaa gaaagcgtga ccggcgttga ccttatgtat gatcgggcgg
     5161 tgcccggtta tataaaatga aaaaaggaga aaaagaaatg aaaaaattgc ttgctctgat
     5221 tttggcggcc gcaatgtcgc tctctcttgc ggcgtgtgga aacggtagca cctcctctgg
     5281 cggagggagc acatcggagc ccgccggaac tcccgcggca tcttcagggg aggagaccgg
     5341 atgggtcccc acagaggaca ttgaatttgt gatccccttc ggcgcgggcg gcggaaatga
     5401 cgttatggtc cgaaaaatcc tggagatcgt tgaaaaatac gatatgtgtc cggtgaatat
     5461 cgtcccggtg aacaaagaag gtggttccgg agtggtaggc tatacctatc tcacctccaa
     5521 gggcgccggg tatgagtatg cgctggcctc cacatccgcc agcttttaca cccagccgct
     5581 gacgggaaat tctcccttta cgccggacga aagcacgttt tccttcgtgg cacatatggt
     5641 aaaagaccct gtggtgctgg tggcccaccc ctcctacggt ttcacatgta tccaggatgt
     5701 ggtcgactat gccaaggagc accccggtga gctgaaatgg ggcggtgtcg gcaacgcatc
     5761 tgacgacgcg atcatcatgt atatgctgaa caatatcgcc gggatcgatc tgacctatgt
     5821 gccctatgac gatgggggaa tgtgccaggc ggcgctgctg ggcggacaca tcggcctgag
     5881 caccgtctct ctggcagagg cggaggagca catggcggca ggcaacatgg tggcgctggc
     5941 cgcctgcgcg gatgagcggt ttgacgcgat ccccgaggtc cccaccgcgg cagagcaggg
     6001 ctttgacatc ggccatcagc agagccgggg catcgtgatg aacgccggcg tgcccgaaga
     6061 ggtgctggcc tattattccg acctgttcca gagagtctct gaaacagagg actggatcga
     6121 gtatgcagag tccaacgcca tgacggtgtc ctttatgcct tatcaggagt tcgcagcata
     6181 tgccgctgag atgctggagg actataccac ctacctgggc atgatccccc aggattgatc
     6241 cagggctgat ctacctggat ctggatgcag ggccggcccc tctttttgat ggggccggcc
     6301 ctgccgtttt gcgccgcccc cttgcattgg gtgcccaaat ccgctacact tatttatcat
     6361 ccttatcatc atccgggaag ggcagagggg gcagagggca tggaccatga ggcatatatg
     6421 cggcgggcgc tggagctggc cgctcaggcg gcggagcagg gggatgtgcc ggtggggtgt
     6481 gtcatcgtcc gggacgggga gatcgtggga gagggacgca accgccggga ggagaatggg
     6541 gacgccaccg cccacgcgga actggaggcc atccgggacg cctgccgccg gctggggagc
     6601 tggaggctgc accggtgcac tatgtatgtc accctggagc cctgccccat gtgtgccggg
     6661 gggatcatca acgcccggat cggcaccgtg cgctatgggg ccaaggatga caaggcgggg
     6721 gcctgcggat cggtgctcaa cctgttcgag gagcggttca accaccgtcc caggctctat
     6781 cggggcccca tggaggcgga gtgcggccgt gccctgcggg cctttttcga ggacctgcgg
     6841 gaaaaagacg gattttcgga ggagatttaa tccttttgta acatctgcgg gtgacttatt
     6901 taataaaaac tggatattat aatactcgca aggcaagagc atcttttcat tctatcctcc
     6961 aattcttttg gaaggccgac aggggactgt cggcctttcc tctttttcca cggctgcggg
     7021 agggaaggga gaaaaaacca gtgcgattta atggttatgt aacatttgcc ccttcatttc
     7081 ttaataactc cagggtatct tgatacttgc aagagacagt ttgtttcatt ttaatcctct
     7141 ttttcagcag aaagtcccga gcgctttgga gcaggcgttc gggactttct gtttttctgc
     7201 atagatttcc agatccaagg gagaatacag ggggaaagga gggcgcatat ggctgaacag
     7261 aagcaggatg gacctcaccc ccgccgggag cccagatttc ccggccccct gaccctggag
     7321 ggagtggagc agatctttga gggctgctcc gactttgcag aacgcaccgc gctgctgcat
     7381 aacgacccac agaggcaggt gaccctctgc tatatcaatg gcatggtgcg gggggagcgg
     7441 atcaacgact atctgctgcg gccgctggcc caggacccgc gcctgtctgc ggtatctgag
     7501 gcggaggcat tccagctgct gggacagggg gcgctctata atttatatgt agagcgccgc
     7561 accacgctgg atcaggcggc agtggatctg actatgggct ggtgtgtcct gttctttccc
     7621 gggcggatgg acgcactctc tttcttcgta gggacagagg aaaagcgggc ggtggggcag
     7681 tcggagaacg agcccgccct gaagggggcc cgggactcct ttgtggagtc catcctcacc
     7741 aacacctccc tggtccgccg ccgcctgcgg gcgccggagc tgaaggtccg gcagcatgtg
     7801 gtcggacggc agtccctcac cccggccatg gtggtctatc tggacgggat cgccgacccg
     7861 gacacggtga agcgggtaga ggagaagctg gacgccatcg acatcgacgg ggtggagtcg
     7921 gcagggaacc tggaggagta cctcaccgat gcgtcccgga ccccctttcc cctgatgccg
     7981 ttcacccagc ggccggaccg gttctgcgcc gggctgctgg aggggcgggt ggggctgctg
     8041 tgtgacggcc tccccttcgg ctatctgctg ccgggcacca tggaccagtt cttccggacc
     8101 agccaggacc gggccttcaa ctggatgact gcctctatgc tgctcctgct gcgctatttc
     8161 tgcatggcgg ccaccctgnn nnnnnnnncg ggctctatat cgccctggtg accttccacc
     8221 cggaggccat cccggtgaag ctggccctgt ccatcgtggc ggccaagcag gaggtcccct
     8281 tctctactgt ctttgaggta ctgattatgc tggtagcctt cgaggtgctc caggaggcgg
     8341 ggctgaggct gccctcgcct atcggagcta ctgtgtccat cctgggcggc ctggtggtgg
     8401 gcaacgccgc ggtggaggcc cacatcgtct ctccggcggt gctcatcgcg gtggccattg
     8461 caggggtggc gggatacacc atgcccagcc aggacatggc ggccgccctg cggctgtggc
     8521 gcttcctgct ggccatcctg gccagcctgg ggggactgtt cggcctggcg atgggctgtg
     8581 gggccctgat ctgccatctg gccgggctgg agagcttcgg cgtgccctat ttggcgccgt
     8641 ttacgtccgc agcaggacag gagcggggga gccctgatct gatccggctg cccctgccct
     8701 ggatgaagtg gcggaacggt gcggcgcggt cggccaaccg gaggaatcag cgatgaaaaa
     8761 agttttagca gcggccctgt gcggcgcggt gatgttcctg ctcccaggat gcggcgggga
     8821 gcggcttccc tatgcccggg agatggggga catggccctg atgcgcacca tgggagtgga
     8881 cacgggggag ggcggagcgg agcagctcca tgttacggtg tccagcggcc gccgggccag
     8941 aggcttgcag ggggaggcgg agccgcccct ggtcctctct gcggagcggg agtccatcag
     9001 cagcgcctgc ctggccatgc aggggctgag tgacagctat gtgttttacg gacatgtgga
     9061 ccaactgctg ctgggggagg agctgtcccg ccggggcatc ctggagaccc tccagtactt
     9121 cagccaggac caggagctgg ggatggggac ccaggtctgg atcgtccggg gcggtacggc
     9181 ggcggaggcc atctccgccg agcaggagcg gggggtggag agccgcctgt ccaccatcca
     9241 aacggacagt gaaatgggga tggcggggat gaccaggacc gtgggggaga ccctgacgtc
     9301 cctgatggag gacggttccg cctatctgcc cgggctggtc cccggggagg acggcgcact
     9361 gctggaggcg ggatacggag tggtgaagga cggcatgctg gcgggctggc tcaccggaga
     9421 gcaggcccgg ggcctggagc tggcggccgg gcaggtcggg gcggacatcc tggagctgga
     9481 aaccagggga ggaggcgccg cagtccgggt gacagcggta tcggtgagct gtgtgccgga
     9541 gttttccggc gagaggctga ctgggctgga gctggcctgc cgcctctctc tgcgggcgga
     9601 agagagctgg ggggaaccgg agccggcaga gctgtgtgcg gcggcggagg agcgggaagc
     9661 ggcccggatc tcagcggcgc tggagcagct gaagggctgg aacgcggact gcctgggcct
     9721 ggccaggcgg gcgggccaga cggagagcgc ccgctggaac gccctccggg cccagtggcc
     9781 ggaggtgttt ccggaactgg agatccaggt gacagtacag gcttcgctgc tggcggcctg
     9841 aggagggaaa agggattgaa acaggagaag gtctcgttcc ggcagctgat ggcgctgctc
     9901 tgggccgggc tgctggggcc ggcggcggag tggctgcctt ccatggtcac agagcgggca
     9961 gggcccgccg ggtggctggt acccctgctg gccctgccgg tgctgctggc cgccgggtgg
    10021 ctggcgggct ccctgagcag gggagcaggg gggctgagcc agggactgat ggacagcttc
    10081 ggacccgggg cagggagagc ggtgctcacg atctatatca tatggatgga actgctgctg
    10141 gccctgcggc tgcgtctgtc tgcccagaga atgatggcgg cgggggagag ggacggcgca
    10201 ctctggtttt tcctgccggt gctggtcctg atggccctct gg
//
LOCUS       DBMDSJ010000010        10012 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2199_length_10012_cov_1.928362, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000010 DBMDSJ010000000
VERSION     DBMDSJ010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2199_length_10012_cov_1.928362"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="ACZPAQ_00630"
     CDS             <1..735
                     /locus_tag="ACZPAQ_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR0902508.1"
                     /translation="SENYILPLSHDEVVHMKGAFFGKMPGENPMKFAGVRVFYTYMLA
                     HPGKKLTFMGAELAQWNEWHYEYSLDWHLLQYEPHRQTQEFFKAANAFYLEQPALWEQ
                     DDSWQGFQWLCADDNQANTVSFLRWDKEGSPLLVVCNFSPVHRKGYRVGTPFAGMWSA
                     VLNTDDVSFGGGGLGDQDPVRTEYIPCHDQDQSMEIDLPPMAAVIYRCTRKFPKRKPK
                     ASKSEKTGPSGKRPAKPGASKRKKEA"
     gene            811..2028
                     /locus_tag="ACZPAQ_00635"
     CDS             811..2028
                     /locus_tag="ACZPAQ_00635"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR0902509.1"
                     /translation="MNEMKKEVVAMLLAGGQGSRLYALTSHVAKPAVPFGGKYRIIDF
                     PLSNCVNSGIDTVGVLTQYRPLELNSYIGSGQPWDLDRSDGGVHILPPYMREGDQGTW
                     YKGTANAIYQNIGFLDLYDPDYVVILSGDHIYKMDYADMVDLHKKTGAACTISVLEVT
                     MDEAKRFGIMNVDENDQIYEFEEKPPQPKSNLASMGIYVFSWQKLRRYLIDDEADPKS
                     SNDFGKNIIPNMLAAGEKLMAYRFHGYWKDVGTINSLWDANMDMLAPHSGIDLYDTSW
                     PIYARTPIKPPHVTGPNAVISHSLVTGGCQVDGSVANSVLFHSVTVEEGAKVEYSILM
                     PGTVVKAGASISYAIVAEDAVVEAGAVVGAPPDDSPGWGIAVVAGGVTVGEKATVTPS
                     AMVREDVKGGERV"
     gene            2025..3143
                     /gene="glgD"
                     /locus_tag="ACZPAQ_00640"
     CDS             2025..3143
                     /gene="glgD"
                     /locus_tag="ACZPAQ_00640"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HIR0902510.1"
                     /translation="MNDLHGILFAYHSDSNLGELTRPRNTCSLPFGGRYRLIDFMLSS
                     YVNAGISDVGLIVHESYQSLLDHVGSGKDWDLSRKHGGLRILPPFGVAERGGSGEYRG
                     NMEALAGIYTYLQNIRQEYVIMGWGDMVVNLPVAEVFQQHLDSGADITILCTQELKGA
                     PRNTVYITQGPDGLVSDLSINPAHAANALESLETYIISKKLLLDMVDYCAAHNIHNFS
                     RGVLLPRLSTLKVLPYLHRGYVARFQSVADYFQHSMDLLDPAVRADLFDPDRPIRTKD
                     QSNPSTYYGPDAKSIHSLVADGCFIEGEVENSILSRGVIVEAGAKVSNSVLMQGTIIR
                     AGASLSYTITDKNVQVNQDRMLMGHSTYPLAISKDSIV"
     gene            3212..4651
                     /gene="glgA"
                     /locus_tag="ACZPAQ_00645"
     CDS             3212..4651
                     /gene="glgA"
                     /locus_tag="ACZPAQ_00645"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR0902511.1"
                     /translation="MRILFASSEVAPFIKTGGLADVAGSLPKALAQEGHDVKVILPLY
                     EGVGEQWRSQMKFEQYYNVTLSWRHVYCGVFSLEQDGVTYWFVDNEYYFKRWQLYGHF
                     DDCERFAYFSRAVIETPGHFGWAPDIIHCNDWQTALVPVYMLEERYHVPELANTKTVF
                     TIHNIEYQGRYGDQVLEDVIGLDRSYLNEGMLGYYKDVNLMKGAIMASNFVTTVSPTY
                     AQELRLPFYAHGLAGVINEQSGKLQGILNGIDTQLYDPAATSGLAANFSARSLVKGKA
                     ECKLALQRAVGLEENADVPIIACISRLVGHKGFSLVTDALHEIMAMNVQMVVLGTGDW
                     QYEAAFREAQVQYPGRFAAQITYSAPLSTAIYAGADLFLMPSVSEPCGLSQMIAMRYG
                     TVPVVRETGGLKDTVTPYNKFEGTGRGFTFSDINAHDMVWVLREAVDLYFNNKKAWRG
                     IQKEDMTADFSWNSSARQYLEIYQRILGW"
     gene            4829..7264
                     /locus_tag="ACZPAQ_00650"
     CDS             4829..7264
                     /locus_tag="ACZPAQ_00650"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR0902512.1"
                     /translation="MAEYNKAQLTEMIVGKLLRNFGRTVDEATPNHMFKACAMVLRDI
                     MSGHQIETSNHVWEAQGRQVHYLSLEFLMGRSLEKNAYNLGLLDTLTQVLEDLGFSAA
                     DLFETEPDAGLGNGGLGRLAACYLDSMTTLEIPATGYSICYELGIFKQKIIDGKQVEL
                     ADNWLGLGDAWLIAKMDEAEEVRFGGRIVDHWVDGHNKPEHVGYTTVLAIPRDMEIAG
                     YKTNHTNTLRLWDAKSPVPVDMSLYSRGEYLKAVEQQAMAEVIAKVLYPDDNHYEGKS
                     LRLKQQYFFVSATAQSIVRQHRAQYGTLRNFHQKHVIQINDTHPTLVIPELMRILLDV
                     EGYSWDEAWHIVTNTVCYTNHTVLAEALERWPQNLIESLLPRIWEILKEIAARYQRQL
                     EGYFGGDMNRVSRMAIIWGGEVRMANLCVCACSAVNGVSALHSDILKRDVFHDAYLRQ
                     PDQFKNVTNGIDHRRWLSQINPKLDALIRECTGSDAYLLHPESISGLEKYKDDSAVLD
                     RLESIKQENKRRFAGYVARESGIILNTDAIFDVQVKRLHEYKRQLLNVLHIVCLYQQL
                     RDDPNMDFTPQTFLFGAKAAPGYHVAKQIIQLINSLAAQINADPVCKDKLQVVFLENY
                     RVSLAEKLMPASELSEQISTAGKEASGTGNMKFMMNGALTIGTLDGANVEMHQQLGDE
                     NIFLFGLTADQVEERRRQGYRSLDYYQQDPVLKRVLDQISAGFSDGRAYTDLTNRLLF
                     GGGGGIADEYMLLADFDSYCQAHRRSLEVYKDRRAWDQKSLINIARSGIFAADRSIRD
                     YARDIWHVPTRFD"
     gene            7459..8262
                     /locus_tag="ACZPAQ_00655"
     CDS             7459..8262
                     /locus_tag="ACZPAQ_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR0902513.1"
                     /translation="MYLADHHIHSCCSPDSEAPLSEMLRAAQAAGLSDLCATDHCDLL
                     DLDGGRVAGLDWAPILTQYQAARRDCPAGVKLRLGLELGGAPVDPDCAAAILSGADLD
                     FVIGSIHNLSPAKGGKDFYFLDYTTPRACYEALDDYFDSLAQLVHLDCWDTMGHIIYP
                     LRYMQKIPGHPVTLERYQDRLRALLTGVAQAGRAIEVNTWCGRTVTQWQPLLELYRDC
                     GGELITLGSDAHRPEDVGKGIAQAQELLRTVGFRYFTVYHRRTPQFIRL"
     gene            8305..8739
                     /gene="rpiB"
                     /locus_tag="ACZPAQ_00660"
     CDS             8305..8739
                     /gene="rpiB"
                     /locus_tag="ACZPAQ_00660"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR0902514.1"
                     /translation="MIAIASDHGGYALKEHIKAYLAAKGITCQDFGCETPDRCDYPVF
                     GKAAAQAVASGQCEKGIVICTTGIGISISANKVKGIRCALCGDTLSAEMCRRHNNANM
                     LAMGAGIVGPNLAERMVDVFLSTEFDGGRHTKRVELMMEIEG"
     misc_binding    8841..9085
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9154..>10012
                     /locus_tag="ACZPAQ_00665"
     CDS             9154..>10012
                     /locus_tag="ACZPAQ_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR0902515.1"
                     /translation="MTGAQALIESLKREQVAHIFGYAGATICPAVDALKSHPEIGYTL
                     VRTEQNAGHMASGYARVSGKVGVCMVTSGPGATNLITGIATAYMDSIPMVAITGQVPS
                     NLLGRDIFQEVDITGAVAPFSKHSYLVKDANDIPRIVKEAFHIASTGRPGPVLIDIPI
                     DVQEQSLKKFYYPEEVSIRGYKPSVKGNDLQIKRVVEAISRSKQPLICAGGGVWLADA
                     KEELLELAERCSIPVVKTMMGLGLLPTDHPLNMGMIGAHGNHCANKALAKADLLIMVG
                     TRAADRAMVD"
BASE COUNT         1997 a         3257 c         2934 g         1824 t
ORIGIN      
        1 tcggagaact acatcctgcc cctctcccac gacgaggtgg tccacatgaa gggcgccttc
       61 tttggcaaga tgcccgggga gaaccccatg aagtttgccg gcgtccgggt cttctatacc
      121 tatatgcttg cccacccggg caaaaagctc acctttatgg gggccgagtt ggcccagtgg
      181 aacgagtggc actatgaata ttccctggac tggcatttgc tccagtacga gccccaccgc
      241 cagacccagg agttctttaa ggcggccaac gccttctatc tggagcagcc cgccctctgg
      301 gagcaggatg actcctggca gggcttccag tggctgtgcg ccgatgacaa ccaagccaac
      361 actgtctcct tcctccgctg ggacaaggag ggcagccccc tgctggtagt gtgcaacttc
      421 tctccggtac accggaaggg ttatcgggtg gggaccccct tcgccgggat gtggtccgct
      481 gtgctgaaca cagatgacgt gtccttcggc gggggaggcc ttggcgacca ggaccctgta
      541 cgcaccgagt acatcccctg ccacgaccag gaccagtcca tggagatcga cctgcccccc
      601 atggccgccg tcatctaccg ctgtacccgc aagttcccca agcgcaagcc caaggcgtcc
      661 aaatcggaaa aaaccggccc ctccggcaag cgccccgcca agcccggcgc ctccaagcgg
      721 aaaaaagagg cataaccgga ccagtttctc cgtttgctcc tggtccgtta tacgatactt
      781 ccctgtgtgt gcttatcaat ccaagaggtg atgaatgaga tgaagaaaga agttgtggca
      841 atgctgctgg ccggagggca gggcagccgg ctttacgccc tgaccagcca tgtagcaaag
      901 cccgcggtcc ccttcggcgg caagtaccgc atcatcgact ttcccctgtc caactgtgtc
      961 aactccggca tcgacacagt gggcgttctg acccagtacc gtccgctgga gctgaacagc
     1021 tacatcggct ctggccagcc ctgggacttg gaccgctccg atggcggcgt acatatcctc
     1081 ccaccctaca tgcgcgaggg agaccagggc acctggtaca aggggaccgc caacgccatc
     1141 tatcagaata tcggcttcct ggacctgtat gatccggact atgtagtcat cctctccggc
     1201 gaccacatct acaagatgga ctatgccgac atggtggacc tgcacaagaa gaccggtgcc
     1261 gcctgcacca tctccgtgct ggaggtcacc atggacgagg ccaagcgctt cggcatcatg
     1321 aacgtggacg agaacgacca gatctatgag ttcgaggaga agccccccca gcccaagagc
     1381 aacctcgcct ctatgggcat ctacgtcttt tcctggcaga agctgcgccg ctaccttatc
     1441 gatgatgagg cagatcccaa gtcttccaac gatttcggaa agaacatcat ccccaacatg
     1501 ctcgccgcag gggagaagct gatggcctac cgcttccatg gctactggaa agacgtgggc
     1561 accatcaact ctctgtggga cgcaaacatg gacatgctgg ccccccacag cggcatcgat
     1621 ctttatgata ccagctggcc catttacgcc cgcaccccca tcaaacctcc ccacgtcacc
     1681 ggccccaacg ccgtgatctc ccactccctg gtcaccggcg gctgccaggt ggacggctcg
     1741 gtggccaact cggtcctctt ccattccgtc acggtggagg agggggccaa ggtggagtac
     1801 tccatcctca tgcccggcac cgtggtgaaa gccggtgcca gcatctccta cgccatcgtg
     1861 gcggaggacg ccgtggtgga ggcgggcgcc gtggtgggcg cgcctccgga cgattctccc
     1921 ggctggggca tcgccgtagt ggcaggcggc gtcaccgtgg gagagaaggc caccgtgacg
     1981 cccagcgcca tggtccggga agatgtgaaa ggaggtgagc gggtatgaac gatctccacg
     2041 gcatcctgtt tgcctaccat tctgactcca atctaggtga gctgacccgc ccccgcaata
     2101 cctgctctct gcccttcggc ggtcggtacc gcctgatcga cttcatgctc tccagctatg
     2161 tcaacgcggg catcagcgac gtgggcctga tcgtccacga gagctatcag tccctgctgg
     2221 accatgtggg ttccggcaag gactgggacc tgagccgcaa gcacggcggc ctgcgcatcc
     2281 tgcccccctt cggcgtggca gagcggggcg gcagcgggga ataccgcggc aacatggagg
     2341 cgctggcggg catctacacc tatctccaga acatccgtca ggagtatgtc atcatgggct
     2401 ggggcgacat ggtggtcaac ctgcccgtgg ccgaagtgtt ccagcagcat ctggacagcg
     2461 gtgctgacat caccatcctc tgcacccagg agctgaaggg cgccccccgc aacaccgtct
     2521 acatcaccca gggtcccgat ggactggtga gcgatctgtc catcaatccc gctcacgcgg
     2581 ccaacgccct ggagtcgctg gagacctaca tcatctccaa aaagctgctg ctggacatgg
     2641 tggactactg cgccgcccat aacatccaca acttcagcag gggcgttctg cttccccgtc
     2701 tgagcaccct gaaggtgctg ccctatctcc acagaggcta tgtggcccgc ttccagtccg
     2761 tggccgacta cttccagcac tccatggacc tgctggaccc agcggtgcgg gcggacctgt
     2821 tcgatcctga ccgccccatc cggaccaagg accagtccaa cccctccacc tactacggcc
     2881 cagacgccaa atcaatccac tccctggtgg cggacggctg cttcatcgag ggcgaggtgg
     2941 aaaactccat cctctcccgc ggcgtcattg tggaggcagg cgccaaggtc tccaacagcg
     3001 tcctgatgca ggggaccatc atccgcgcag gtgcctccct gtcctacacc atcaccgaca
     3061 agaatgtcca ggtgaatcag gaccggatgc tgatgggcca ctccacctat ccgctggcca
     3121 tctccaagga ttccatcgtc tgatcgcagg ggaggcccct gaccggacaa ggggcctccc
     3181 tccgcgctga tatgaagaaa ggaactgatc tatgagaatt ctgtttgcct cgtctgaggt
     3241 ggcgcccttt atcaagaccg gcgggctggc cgatgtggcc ggctctctgc ccaaggcgct
     3301 ggcccaggag ggccacgacg tcaaggtcat cctaccgctg tatgaggggg tcggagaaca
     3361 gtggcgcagc cagatgaagt tcgagcagta ttacaacgtc actctgtcgt ggcggcacgt
     3421 gtactgcggt gtcttctccc tggagcagga cggcgtcacc tactggttcg tggacaatga
     3481 gtattacttc aaacgctggc agctgtacgg ccactttgat gactgtgagc gctttgccta
     3541 cttctcccgg gccgtcatcg agacgccggg ccacttcggc tgggccccgg acatcatcca
     3601 ctgcaacgac tggcagaccg ccctggtccc ggtctacatg ctggaggagc ggtaccacgt
     3661 ccccgagctg gccaacacca agacggtgtt caccatccac aacatcgagt atcagggccg
     3721 ctacggcgac caggtactgg aggacgtcat tggcctggac cgcagctatc tcaacgaggg
     3781 gatgctgggc tactacaagg acgtcaacct gatgaagggc gccatcatgg cctccaactt
     3841 cgtcaccacc gtctcgccca cctacgccca ggagctgcgt ctgcccttct atgcccacgg
     3901 cttggccgga gtcatcaacg agcagagcgg aaagctccag ggcatcctca acggcatcga
     3961 tacccagctc tatgaccccg ccgccacctc cgggctggcc gccaacttca gcgcccgctc
     4021 cctggtgaag gggaaggccg agtgcaagct ggccctccag cgggccgtgg gcctggagga
     4081 gaacgccgac gtgcccatca tcgcctgcat ctcccgcctg gtgggccaca agggattctc
     4141 cctggtcact gatgccctcc acgagatcat ggccatgaat gtgcagatgg tggtcttggg
     4201 caccggagac tggcagtatg aggccgcctt ccgtgaggcg caggtccagt atcccggccg
     4261 ctttgccgcc cagatcacct attccgcgcc tctgtccacc gccatttacg ccggcgcgga
     4321 cctgttcctc atgccctcgg tcagcgagcc ctgcggcctg agccagatga tcgctatgcg
     4381 ctacggcact gtccccgtag tgcgggagac cggcggcctg aaggacaccg tcacccccta
     4441 caacaagttc gagggcaccg gccggggctt taccttctct gacatcaacg cccacgatat
     4501 ggtctgggtc ctgcgggagg cagtggacct ctatttcaac aacaaaaagg catggcgcgg
     4561 catccagaag gaggacatga ccgccgactt cagctggaac agctccgccc gccaatatct
     4621 ggagatctat cagcgtatcc tgggctggta gattcagggc ccgtctgggt atcccgccgg
     4681 aaacgggctg aaatggcggc tgttttggcc cttccgccaa ttgacgcgcc cactccggtc
     4741 tgctacaatg gagcctgtat ggaaaaattt ttcgccatac aggctccgtg atttttattt
     4801 tcattttaaa ttaacaggga ggcaaattat ggctgaatac aacaaagccc agctcactga
     4861 gatgatcgtg ggcaagctcc tgcggaattt tggccgcaca gtggatgagg ccacccctaa
     4921 tcatatgttc aaggcctgcg ccatggtcct gcgggatatc atgtccggtc atcagatcga
     4981 gaccagcaac cacgtctggg aagcccaggg ccggcaggtc cactacctct ctctggaatt
     5041 cctgatgggc cgctctttgg agaagaacgc ctataatctg gggctgctgg acaccttgac
     5101 ccaggtgctg gaggatctgg gcttttccgc cgctgacctg ttcgagaccg agccagacgc
     5161 cggcctgggc aatggcggtc tgggtcgtct ggccgcctgc tacctggact ccatgaccac
     5221 tttggagatc ccggccaccg gctattccat ctgctatgag ctgggcatct tcaagcagaa
     5281 gatcattgac ggcaagcagg tggagctggc cgacaactgg cttggcctgg gcgacgcctg
     5341 gcttatcgcc aagatggacg aggcggagga ggtccgcttt ggcggcagga tcgtggacca
     5401 ttgggtggac ggccacaata agccggagca cgtgggctac acgaccgtgc tggccattcc
     5461 acgtgacatg gagatcgccg gctacaagac caaccacacc aacaccctgc gcctgtggga
     5521 cgccaagagc ccggtccccg tagatatgtc cctctactcc cggggtgagt acctgaaggc
     5581 ggtggagcag caggccatgg ccgaggtcat cgcaaaagtg ctctatcccg atgacaacca
     5641 ctacgagggc aagtccctgc ggctgaagca gcagtatttc ttcgtctccg ccaccgccca
     5701 gtctatcgtc cgccagcacc gtgcccagta tgggacgctg cgcaacttcc accagaagca
     5761 tgtcatccag atcaacgaca cccatcccac cctggtcatc cccgagctga tgcgcatcct
     5821 gctggacgtg gaggggtaca gctgggacga ggcgtggcac atcgtcacca acaccgtctg
     5881 ctacactaac cacaccgtcc tggccgaggc cctggagcgc tggccccaga acctgatcga
     5941 gtccctgctg ccccgcatct gggagatcct gaaggagatc gccgcccgct accagcgcca
     6001 gctggaggga tacttcggcg gggacatgaa ccgggtctcc cggatggcca tcatctgggg
     6061 tggggaggtc cgcatggcca acctgtgcgt gtgcgcctgt tccgcagtca acggcgtgtc
     6121 cgccctccac tccgatatcc tcaagcggga cgtgttccat gacgcctacc tccgccagcc
     6181 tgaccagttt aaaaacgtga ccaacggcat cgaccaccgc cgctggctct ctcagatcaa
     6241 ccccaagctg gacgccctga tccgggagtg caccggcagc gacgcctacc tgctccaccc
     6301 ggaatccatc tccggcctgg agaagtacaa ggacgactcc gccgtcctgg accgcctgga
     6361 gtccatcaaa caggagaaca agcgccgttt tgccggctat gtggcccggg agtccgggat
     6421 catcctcaac accgacgcca tcttcgacgt acaggtgaag cgcctccatg agtataagcg
     6481 ccagctgctc aacgtgctgc atatcgtgtg cctgtaccag cagctgcggg acgatcccaa
     6541 tatggacttc acgccccaga ccttcctctt cggcgccaag gcggccccgg gctaccacgt
     6601 ggccaaacag atcatccagc tcatcaactc cctggccgcc cagatcaacg ccgaccccgt
     6661 ctgcaaggat aagctccagg tggttttcct ggagaactac cgggtctccc tggcagagaa
     6721 gctgatgcct gcctccgagc tctccgagca gatctccacc gccggcaagg aggccagcgg
     6781 caccggcaac atgaagttca tgatgaatgg cgccctcacc atcggcaccc tggacggagc
     6841 caatgtggag atgcaccagc agctggggga tgagaacatc ttccttttcg gcctcaccgc
     6901 cgaccaggtg gaggagcgcc gccgccaggg ctatcgctct ctggattact accagcagga
     6961 cccggtgctc aagcgggtgc tggaccagat ctccgccggc ttctccgacg gcagggccta
     7021 caccgacctg accaaccgcc tgctcttcgg cggagggggc gggatcgccg atgaatatat
     7081 gctactggcc gactttgaca gctactgtca ggcccaccgc cgctctctgg aggtctacaa
     7141 ggaccggaga gcctgggatc agaaatctct catcaacatc gcccggtccg gcatcttcgc
     7201 cgccgaccgc tccatccggg actacgcccg ggacatctgg catgtcccca cccggttcga
     7261 ctgacccttt caaagccgcc gcagacgggt ctgcggcggc tttttccgtc cggcttagcg
     7321 ctggctggaa cgccgttcct cttccggaaa tggtaggaag ggatttccaa gaaaatcact
     7381 tgcaaaatgt caacaaatgc aataaaatag agggcgatgc ggactgatcc acagaatttg
     7441 cccgccggag gtctctgtat gtatcttgct gatcatcaca tccattcctg ctgctccccg
     7501 gacagtgagg cgcccctctc ggagatgctc cgggcggccc aggccgccgg cctctccgac
     7561 ctgtgtgcca ctgaccactg cgacctgctg gatctggacg ggggacgggt cgcagggctg
     7621 gactgggccc ccatcctgac ccagtatcag gcggctcgga gggactgccc cgccggcgtg
     7681 aagctgcgcc tgggtttgga gctgggcggt gccccggtgg acccggactg cgccgccgcc
     7741 atcctctccg gcgcggacct ggactttgtt atcggctcca tccacaacct cagccccgcc
     7801 aaggggggga aggacttcta tttcctggac tacaccaccc cccgggcctg ctatgaggcc
     7861 ctggatgact acttcgacag cctggcccag ctggtccatc tagactgctg ggacacgatg
     7921 ggccatatca tctaccccct tcggtatatg cagaagatcc ctgggcatcc tgtcactctg
     7981 gagaggtacc aggaccgcct gcgcgcgctc ctcaccgggg tggcccaggc ggggcgggcc
     8041 attgaggtga acacctggtg cgggcgcact gtgacccagt ggcagcccct gctggagctc
     8101 taccgggact gcggcggcga gctgatcacc ctggggtccg acgcccacag gccagaggac
     8161 gtggggaagg ggatcgccca ggcccaggag ctgctccgga cagtcggctt ccgttatttc
     8221 accgtctacc accgccggac cccccaattc atccggctgt gacagaagga tagttgaaga
     8281 agagagaaga aggaggattt cattatgatc gcaatcgctt ccgaccacgg agggtacgcc
     8341 ctgaaggagc acatcaaggc ctatctcgcc gctaagggga tcacctgcca ggacttcggc
     8401 tgtgagacgc cggaccggtg cgattaccct gttttcggca aggctgccgc ccaggccgtg
     8461 gcctccggcc agtgtgagaa ggggatcgtc atctgcacca ctggcatcgg catctccatc
     8521 tctgccaaca aggtaaaggg catccgctgc gccctgtgcg gggataccct gtccgccgag
     8581 atgtgccgcc gccacaacaa tgccaacatg ctggctatgg gggcgggtat cgtgggcccc
     8641 aacctagcgg agcggatggt ggacgtgttt ctgtccaccg aattcgacgg cggccgccac
     8701 accaagcggg tagagctgat gatggagatc gagggctgag cccagcagaa aaatgttccg
     8761 gagaaaaaaa gggaattgac aaacagctcc ccgtgcgata aagtagtaaa taacttttac
     8821 cggggcgacc cggaagatga caaaacgcga tgaccgggcc agtaggcagt tccaaagggt
     8881 acagagaggg agcggttggt gtaagctcca cctgtggaac agggcgccga acatcacccg
     8941 cgagcagtcc cgctgaaccg tttgccgcag taggcggggc cggagaccca ccgttacagg
     9001 ggcatgagcg cgcagcgccg gcctgttctg gccgctccgc tgtgaagtta ggtggtacca
     9061 cggagcagac ggcttcgtcc tatcgagata ggacgaagcc gtttattttt atcccgccgc
     9121 ccaggcggca tgcaatttgg aggtgatctt ctcatgacag gagcacaggc cctgatcgag
     9181 agtttgaagc gggaacaggt agcgcatatc ttcggctatg ccggcgccac catctgcccg
     9241 gcggtggacg ccttgaagtc ccacccggag atcggctaca ccctggtccg cacggagcag
     9301 aacgcgggcc atatggcctc cggctatgcc cgggtcagcg ggaaggtggg ggtgtgcatg
     9361 gtgacctccg gcccgggcgc caccaacctg atcaccggca tcgccaccgc ctatatggat
     9421 tccatcccca tggtagccat caccggccag gtacccagca atctgctggg gcgggatatc
     9481 ttccaggagg tggatatcac tggcgcggtg gcccccttct ccaagcacag ctacctggtc
     9541 aaggacgcca acgacatccc ccgcatcgtc aaggaggcct ttcacatcgc ctccactggc
     9601 cggcccggcc cggtgctcat cgacatccct atcgatgtac aggagcagtc cctgaaaaag
     9661 ttctactacc ccgaggaggt cagcatccgg ggctataagc ccagcgtaaa gggcaatgac
     9721 ctgcagatca agcgggtggt ggaggccatc tcccgctcca aacagcctct gatctgcgcc
     9781 ggcggcggcg tgtggctggc cgacgccaaa gaggagctgc tggagctggc ggagcggtgc
     9841 tccatccctg tggtcaagac catgatgggg ctggggctcc tgcccaccga ccacccgctg
     9901 aacatgggga tgatcggggc ccacggcaac cactgcgcca acaaggccct ggccaaggcg
     9961 gacctgctca tcatggtggg cacccgggcc gccgaccggg ccatggtgga cc
//
LOCUS       DBMDSJ010000011        10008 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2201_length_10008_cov_1.727062, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000011 DBMDSJ010000000
VERSION     DBMDSJ010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10008)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_10008_cov_1.727062"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="ACZPAQ_00670"
     CDS             <1..1096
                     /locus_tag="ACZPAQ_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0902516.1"
                     /translation="RDAEEDFREELNHSRRAWQASQERRSVEPCHIREVLSQWTGVPV
                     TDLDDGDRRALLELEDHLRGQLLGQDAAVEQVARAIRRGRLGLKDSRRPTGCFLLLGP
                     SGVGKTQLCRALAAVLFGSQDALIRFDMSEYMEPHSVSRLIGSPPGYVGHEDGGQLTE
                     AVRRSPWSVVLLDELEKAHRDIWSILLQVMEEGVLTDSRGRKTDFRNTVLVMTSNLGS
                     QRFVRGPGLGFSPGPEGDRTALEREVLADARKSFPPEFLNRLDAALVFHPLDHTSLVS
                     ITRQLLDQAGARLAELGVSLSAEEGAVELLARSGGDREYGARPLRRAVSSLVEDPAAD
                     LVLRGALVPGDTLQVSVSQGKVTVRPVSPK"
     gene            1236..2669
                     /gene="malQ"
                     /locus_tag="ACZPAQ_00675"
     CDS             1236..2669
                     /gene="malQ"
                     /locus_tag="ACZPAQ_00675"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR0902517.1"
                     /translation="MEDHRSSGILLAVSSLPGPYGIGSLGGPARRFVDFLAEAGQHYW
                     QILPLVPPGDGSSPYMSPSAFAGNPYLIDLDELAAEGLLTPQELDGARWPDPDRVDYD
                     HLAQTRIPLLRKAWERSQTAHLQSDAPDLPWLEDYCTFAALHDRYQAPFWQWPEDAPP
                     PEPEEIDFHRFLQETFYRQWMALKKYASRRCIRIMGDIPFYLSPDSVQMWRQPELFQL
                     DGKGHLAASAGVPPDAFSDQGQLWGNPLYDWKGNKQGVFDFWKRRIQWCAAIYDAVRI
                     DHFRAFHSYWSVPTGAENAREGHWEDGPGMELLHALQKSAPQLELIAEDLGDLGPEAL
                     QFVRTCGIPGMKVMVFAFDPVGESAYLPHNCQRDSVIYTGTHDTPTFLQWLRETDPDS
                     RLFASRYLRLRQDEGLGWGVISGAWAAPSRLAIAPLQDVLGLGSDARMNTPGTMGSQN
                     WSWRVRQEAFNPYVSGKLREITRTYRR"
     gene            2785..3792
                     /locus_tag="ACZPAQ_00680"
     CDS             2785..3792
                     /locus_tag="ACZPAQ_00680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HIR0902518.1"
                     /translation="MTYSEVLQAARGCSGPYCKACPVCNGRACGSSMPGPGAKGTVAV
                     RNYEAWQSIRLDLDTICENKPVDTRYTFFGQQYDLPVFAGPVGATKLHYGEKYDDQAY
                     NSILVPACAQAGIAAFTGDGTDPAVFTAAARAIGASGGKGIPTVKPWDRNTLFSKLDE
                     AKAAGAQVIAMDIDAAGLPFLKGLQPPAGSKTVTELREIVEYAEVPFLIKGVMTVKGA
                     RKALEAGAAGIVVSNHGGRVLDGVPATAEALPAIADAVRDQMVILVDGGIRSGVDVCK
                     ALALGADAVLLARPYVTAVYGGGAEGVALLTQKLKGELEDTMAMCGVHSLKEITREIL
                     F"
     gene            complement(3907..6591)
                     /locus_tag="ACZPAQ_00685"
     CDS             complement(3907..6591)
                     /locus_tag="ACZPAQ_00685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR0902519.1"
                     /translation="MSEEQNSRLYNENNDYGERDYAPRKSGKKKKRKESGGHVGRCIL
                     QVLGTLLLVGLCTGAILCCFAAVYIKTVIIPEAGAVDLSQFAVGENSVMYYMDQDTGN
                     YRELVTLAAEENAEWVNYEDIPEDLINATVAIEDRRFWEHNGVDWKRTAAAILYMFTG
                     QDIQGGSTITQQLIKNLTQYDDVTVKRKVIEIFRALQFDKDYTKETTLEWYLNFIWLG
                     DRCRGVGAAAMNYFGKPVQELTLAECASLISITNNPTIYGPYSDAVFTNSETGEQKTA
                     RDKNKERQELVLWSMLDQGYITQEEYDEAVAQELVFDRAAGESTPSTIYSWYEEQVIS
                     DVKDDLKAQYGYSDEAVSLLLTRGGLSIYTCVDPDVQAQVEQIYSDRTNLDYTSSSGQ
                     QLQSAITVIDNETGNLVGIAGRVGEKTGNLWMNFATDSKRQPGSSIKPLSVYAPAFEM
                     GLISPISVLDDYPHEVMGGRPWPVNVDGRYRGLVTVRQALSNSYNTVSVRVLADLVTP
                     ENSFNFVEEHFHLDLEDGVEIGGQMKSDIGVAPLSMGGLTYGVNTRDMAEAFAVFPNG
                     GLYQKSRTYTKVTREVDGVETVLLENQPESEAVLKSETAYYVNSILREVVTRVNTSGN
                     FDSGMAIAGKTGTTDDKYDRWFVGYTPYYTAAVWVGFEQNERVPASGNPALKMWTLVM
                     SGVHEGLEDRKFSEPAGLTTVTYCLDSGLLATDYCRMDPRGDRTARGTVFQGDAPTEF
                     CTSHTAETTVTVCLDCPILDGNGEETGLYHLAGEFCPEESRQEVSLLGYQREDVGGAG
                     AEDAKYFLGVTQEAGPCTVHTAAQPDPDLPFDPNQPWDPSDPWNPNTNPLDPTGQGGG
                     GAADPGQEDPDAPQGGETEDPSGTDPIINPETGRPYGY"
     gene            6843..7160
                     /locus_tag="ACZPAQ_00690"
     CDS             6843..7160
                     /locus_tag="ACZPAQ_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR0902520.1"
                     /translation="MSEEFGPDFITVTDEDGNDFELEHLDTLEYNNEVYMAFFPAVSE
                     DAQEGEPADIDLDDEEYGLIIMKVVEEDGEELLSTLDSDEELDLIYNQFMERLFADEE
                     MED"
     gene            7169..7366
                     /gene="ssrS"
                     /locus_tag="ACZPAQ_00695"
     ncRNA           7169..7366
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACZPAQ_00695"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7582..9144
                     /locus_tag="ACZPAQ_00700"
     CDS             7582..9144
                     /locus_tag="ACZPAQ_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012846.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR0902521.1"
                     /translation="MSASREKKSRQDPSGQGLSEKKLKQQQEGRQTHVHHIVYTVLAV
                     LVVLFGVGLLIWNSNFFQSRAAAVTIDGQDYTAADVQYYYNGALQQEIYNSYSGASTM
                     DYTLDPEDQIYDESTGETWRDHLLDVAIQTLTQQAAIANEAQASGYTMSAQAQESLQN
                     TLDSIQAGTITSGYGSKDAYVRANYGPTMSYDKFVQIMERYYLAADYAQSQVDSYTYD
                     DSQLDAYYEEHADELDTFTLSQFVFQARVNTVDDEGNTIEMTDEEKAAALEEEKAAVK
                     EQAEALQARLEAGEDPEALAEEFSDSIYSSEVALEQMGSTVNSEYSEWAYDSARRPGD
                     ITLVEYAGGSSESYNYCVALFHDRVRDDAPTADVRHILIGAADANITPTEEQYEQAKA
                     KAEELLAQWEAGDATEDSFAQLAQENSADTSSASNGGLLQYVSPYSGYVDTFTQWSLD
                     PSRQPGDTGIIQNTGSSVKGYHIMYFAGWDDPVWAVSAKNALNNEQYSQWAEKLASAC
                     TVTRGSGLKYVE"
     gene            complement(9204..>10008)
                     /locus_tag="ACZPAQ_00705"
     CDS             complement(9204..>10008)
                     /locus_tag="ACZPAQ_00705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR0902522.1"
                     /translation="PPGEDDPQEPGERAESPTISYRDRELPVLEGVAVNSYVSDGFSV
                     NDRGWLTYEQDGKQAAIGIDVSAYQGEIDWQQVADSGVEFAMIRLGYRGYSQGVIMPD
                     KNFEQNLRGALDAGLEVGVYFFSQAVSVWEAEEEAQYVLDAIQGYDVTYPVAFDWEFI
                     AGDTARTDGMEPEAITRCAGAFCDMIREAGYHPVVYFNQDLGYLSYQLDRLTDYTFWL
                     AEYNARPSFFYHFDLWQYTHTASVPGIEGNVDMNLAFREFDEKPQHGEE"
BASE COUNT         1822 a         3279 c         3022 g         1885 t
ORIGIN      
        1 ccgggacgcg gaggaggatt ttcgggagga gctgaatcac tcccgccggg cctggcaggc
       61 cagccaggag cggcgctcgg tggagccctg ccacatccgg gaggtcctct cccaatggac
      121 cggggtgccg gtcaccgatc tggacgatgg ggaccgccgg gcccttttgg agctggagga
      181 ccatctgcgc gggcagctgc tgggccaaga tgccgcagtg gaacaggtgg cccgagccat
      241 ccgacgggga cggctgggac taaaagacag ccgccggccc accggctgct tcctcctcct
      301 gggtcccagc ggcgtgggca agacccagct gtgccgggcc ctggccgctg tcctcttcgg
      361 cagtcaggac gccctgatcc gcttcgacat gtcggagtat atggaacccc actccgtctc
      421 ccgcctcatc ggctcccccc cgggctatgt gggccatgag gacggcggac agctcaccga
      481 ggcggtgcgc cgctccccct ggtccgtggt gctgctggac gagctggaga aggcccatcg
      541 ggatatctgg tccatcctgc tccaggtgat ggaggagggg gtgcttaccg acagccgggg
      601 ccgcaaaacg gatttccgca acaccgtgct ggtcatgacc tccaacctgg gttcccaacg
      661 cttcgtgcgg ggacccggcc tgggcttctc ccccggccct gagggggacc ggaccgccct
      721 ggagcgggag gtgctggccg acgcacggaa gtcctttccc ccggagttcc tcaaccgtct
      781 ggacgccgcc cttgtcttcc acccactgga ccacacttcc ttggtctcca tcacccggca
      841 gctgctggac caggcggggg ctcgcctggc ggagctgggc gtctctctgt ctgcggagga
      901 gggtgcggtg gaactgctgg cccgcagtgg cggggaccgg gagtacggcg cccgccctct
      961 gcgccgcgcg gtctcctctc tggtggagga ccccgccgca gacctggtgc tgcggggtgc
     1021 cctggtcccc ggagacacgc tccaggtctc cgtctcccaa ggcaaggtca cagttcgccc
     1081 agtttccccg aaatgaacgg aaatattgca caattttccc tttcctttcc ttttcttttg
     1141 tggtataatg cccataaggc gacccctcag cacatgcacg gccccgctgg tcaccagcgg
     1201 ggcttgccat attcccgttt aggaggcatc gtttcatgga agatcatcgt tccagcggca
     1261 tcctgctggc cgtgtcctct ctgcccggcc cctacggcat cggctccctg ggcggacccg
     1321 cccggcggtt cgtggatttt ctcgccgagg ccgggcagca ctactggcag atcctgcccc
     1381 ttgtcccacc gggggacggc tcctccccct atatgtcccc ctccgctttt gccggcaacc
     1441 cctatctcat cgacctggat gaactggcgg ccgaggggct cctcaccccc caggagctgg
     1501 acggggcccg ctggcccgac ccggaccggg tggattacga ccatctggcc cagacccgta
     1561 tccccctgct gcggaaggcc tgggaacgct cccaaaccgc ccacctccag tctgacgccc
     1621 cagacctgcc ctggctggag gattactgca ccttcgccgc cctccacgac cggtatcaag
     1681 cccctttctg gcagtggcct gaggacgccc ctccgccgga gccggaggag attgattttc
     1741 accgtttcct ccaggagacc ttctaccgcc agtggatggc tctgaagaaa tatgccagcc
     1801 ggcgctgtat ccgcatcatg ggtgacatcc ccttctacct ctccccagac agcgttcaga
     1861 tgtggcgcca gcccgagctc tttcagctgg atgggaaggg ccatctggcc gcctcggccg
     1921 gcgttccccc cgacgcattt tctgaccagg gccagctgtg gggcaacccc ctctatgact
     1981 ggaagggaaa caagcagggg gtctttgact tctggaagcg gcgcatccag tggtgcgccg
     2041 ccatctatga cgccgtgcgt atcgaccact tccgggcttt ccacagctac tggtccgtcc
     2101 ccactggcgc ggagaatgcc cgggagggcc attgggagga tggaccgggg atggagctcc
     2161 tccatgccct gcaaaaatcc gccccccagc tggagctcat tgcggaggac ctgggggacc
     2221 tggggcccga agccctgcag ttcgtccgta cctgcggcat ccccggcatg aaggtgatgg
     2281 tgttcgcctt cgaccccgtg ggggagagcg cctacctccc ccacaactgc cagcgggact
     2341 ccgtcatcta tactggcacc catgacaccc ccaccttcct ccagtggctg agggagaccg
     2401 atcccgactc ccgcctgttc gccagccgct atctccggct ccggcaggac gaggggctgg
     2461 gctggggtgt gatctccggc gcctgggccg ctcccagccg cctggctatc gcccccctcc
     2521 aggatgtact ggggctgggc agcgacgctc ggatgaacac ccccggcact atgggcagcc
     2581 agaattggag ttggcgggtc cgtcaggagg ccttcaaccc ctacgtctcc ggtaagctgc
     2641 gtgagatcac ccgcacctac cgccgctgaa acagcagagc agcccgccgt gccccttgga
     2701 gggctcggcg ggctgtcctg ttcttcttga cgtgccgcct cccagccgat acaataacga
     2761 gagaaaacgc gaggaggaag atccatgacc tattctgaag tcctgcaggc cgccagggga
     2821 tgcagcggcc cttactgcaa agcctgcccg gtatgcaatg gccgggcctg cggcagctcc
     2881 atgcccggcc cgggagcaaa gggcaccgtc gccgtccgga attatgaggc gtggcagagc
     2941 atccggctgg atctggacac catctgcgaa aacaagcctg tggatacccg ttacaccttc
     3001 ttcgggcagc agtacgatct gcccgtcttt gccggcccgg tgggcgccac caagctgcac
     3061 tacggcgaaa aatatgacga ccaggcctac aacagcattc tggtcccggc ctgtgcccag
     3121 gccggcatcg ccgccttcac cggcgacggg accgaccccg cagtcttcac cgccgcagcc
     3181 agggccatcg gtgcctccgg cgggaagggc attcctaccg taaagccctg ggaccgcaac
     3241 acactcttct ccaagctgga cgaggccaag gcggccggcg cccaggtcat cgccatggac
     3301 atcgacgccg ccggcctccc cttcctgaag gggctccagc ccccggcggg cagcaagacg
     3361 gtgacggagc tgcgggagat cgtggagtac gccgaagtcc ccttcctgat caaaggcgtt
     3421 atgaccgtga agggggcccg caaggctctg gaggccggcg cggcgggcat cgtggtatcc
     3481 aaccatgggg gccgggtgct ggacggtgtc cccgccacgg ccgaagccct tcccgccatt
     3541 gccgacgcgg tgagggatca gatggtcatc ctggtggacg gcggtatccg cagcggcgtg
     3601 gacgtgtgta aggcactggc gctgggtgct gacgccgttc tgctggcccg cccctatgtc
     3661 acggcggtgt acggcggagg agcagagggc gtggccctgc tgacccagaa gctgaagggg
     3721 gagctggagg acaccatggc tatgtgcggg gtccacagtc tgaaagagat caccagagag
     3781 atccttttct aagtaaaata tagagataac aagcggctcc cacgtgtctt cggcatatgg
     3841 gatccgctct gtttgcagaa cgaaaaacgg gcccgcggag cgatgctccg cgggcccgtt
     3901 ttttcttcaa tatccgtagg gcctccctgt ctccgggttg atgatggggt ccgtcccgct
     3961 ggggtcctcc gtctctccgc cctgaggcgc gtccgggtcc tcctgtccag ggtcggcggc
     4021 acccccgccg ccctgccctg tgggatccag tgggttggtg ttgggattcc acggatcgct
     4081 ggggtcccat ggctggttgg gatcaaaggg cagatccggg tcgggctggg ccgcagtgtg
     4141 gaccgtacag ggcccggcct cctgggtcac acccagaaaa tacttggcgt cctctgcgcc
     4201 ggcgcctccc acatcctccc gctggtagcc cagcaggctc acttcctgcc ggctctcctc
     4261 cgggcagaat tccccggcca ggtggtacag gccggtctcc tcaccgtttc catccaggat
     4321 ggggcagtcc aaacataccg taacagtggt ctccgccgtg tggctggtgc agaattcggt
     4381 cggcgcatcg ccctggaaga cggttcctct ggctgtacgg tccccccgcg ggtccatccg
     4441 gcagtagtcg gtggccagca ggccgctgtc caggcagtag gtgacggtgg tcagcccggc
     4501 gggctcagag aacttccggt cttccagccc ctcgtggaca ccggacatga ccagagtcca
     4561 catcttcagg gccgggttcc cggaggcagg cacccgctcg ttttgctcaa agcccaccca
     4621 gaccgccgcg gtgtagtagg gggtgtagcc cacgaaccag cggtcgtact tgtcatcggt
     4681 agtaccggtc ttgccggcga tggccatgcc gctgtcaaag ttgcccgacg tattgacccg
     4741 ggtgaccact tcccgcagga tggagttgac atagtaggcg gtctccgact tgagcacggc
     4801 ctcggactca ggctggtttt ccagcagtac ggtctccacg ccgtccacct cccgggtcac
     4861 cttggtgtag gtgcgggact tctgatagag gccgccgttg gggaacaccg cgaaagcctc
     4921 cgccatgtcc cgggtgttga cgccgtaggt caggccgccc atggaaaggg gggccacccc
     4981 gatgtcgctc ttcatctggc cgcctatctc cacaccatcc tccaaatcga ggtggaagtg
     5041 ctcctccaca aagttgaagc tgttctccgg ggtgaccaag tcggccagca cccgcacgga
     5101 cacggtgtta taggagttgg acagggcctg acgcacggtg accagtcccc ggtagcggcc
     5161 gtccacgttc accggccagg gccggccgcc catcacctcg tggggatagt catccagcac
     5221 ggagatgggg ctgatgaggc ccatctcaaa ggcgggggcg tacaccgaca gaggcttgat
     5281 ggaggagccg ggctgacgct tgctgtcggt ggcgaagttc atccacaggt tgcccgtctt
     5341 ctcccccacc cggccggcga tgcccactag gttgcccgtc tcgttgtcga tgacggtgat
     5401 ggcggactgg agctgctgac cgctgctgga agtgtagtcc aggttggtgc ggtcgctgta
     5461 tatctgctcc acctgggcct ggacatccgg gtccacacag gtatagatag acaggccgcc
     5521 tctggtcagc agcagggaga ccgcctcatc gctgtatccg tactgggcct tcaggtcgtc
     5581 cttcacatcg gagatgacct gctcctcata ccaggagtaa atggtagagg gagtggactc
     5641 ccccgccgcc cggtcgaaga ccagctcctg ggccacggcc tcatcatact cctcctgagt
     5701 gatatagccc tggtccagca tgctccacag caccagttcc tgacgctcct tgttcttgtc
     5761 ccgggcagtc ttctgctccc cggtctccga gttggtgaag acagcatcgg agtaagggcc
     5821 atagatggtg gggttattgg tgatggagat caggctggcg cactccgcca gggtgagctc
     5881 ctgcacgggc ttgccaaagt agttcatggc cgccgcccct acaccccgac agcggtcccc
     5941 cagccagatg aagttgaggt accactccag agtggtctct ttggtgtagt ccttgtcgaa
     6001 ctggagggct cggaagatct cgatgacctt ccgcttgacg gtgacatcgt cgtactgggt
     6061 caggtttttg atgagctgct gggtaatggt ggagccgccc tggatatcct gaccagtgaa
     6121 catgtagagg atggccgcag cggtgcgttt ccagtccaca ccgttgtgct cccagaaccg
     6181 ccggtcctca atggccacgg tggcgttaat caggtcttcg gggatgtcct catagtttac
     6241 ccactcggcg ttctcctctg ccgccagagt gaccagctcc cggtagttcc cggtgtcctg
     6301 gtccatgtag tacatcacgc tgttttctcc caccgcgaac tggctcaggt ccacggctcc
     6361 ggcctccggg atgatgaccg tcttgatata gacggcggcg aaacagcaca ggattgcccc
     6421 cgtacacagc cccacaagca gcagggtgcc cagcacctga aggatgcacc tgcccacatg
     6481 gccgccgctc tccttccgct tcttcttttt tccggacttg cggggggcgt aatcccgctc
     6541 cccgtaatcg ttgttctcgt tatataaacg actgttctgt tcttctgaca cgttacttcc
     6601 tccgttgggg acaggccccc gggcagttga tagatcctcc gctccggcag ccgccccgga
     6661 cggggcggga caccggatat ctagcatatt atatcataat attcccgttt gtccaccttt
     6721 atgatttcca ccgggcaaaa ggtcgctttt ctcccctgtg aaaatttggg gcctccgggc
     6781 cttgcttttt tcttcaagtc ggggtacaat aggtccagaa caaaacagcg ggagggatct
     6841 ccatgagtga agaattcggc cctgacttta ttaccgtcac cgacgaggac ggcaatgact
     6901 tcgagctgga gcacctggac accctggagt acaacaacga agtctacatg gcctttttcc
     6961 ccgccgtctc agaggatgcg caggagggcg agcctgctga catcgacctg gatgacgagg
     7021 agtacggcct gatcatcatg aaggtggtgg aggaggacgg cgaggagctg ctgtccaccc
     7081 tggacagcga tgaagagctg gacctgatct acaaccagtt tatggaacgt ctctttgcgg
     7141 acgaagagat ggaggattga ccgaactgga tctcctgctc ggtgcgtgat gggctctttt
     7201 aaacccacat ttctaaaacg ggacaccccc tcacagcgtg gcttgcaggc ctcccggctg
     7261 cccctgcggc atgctggaag ttggaagcag taaagcgctg tggaggtgac ccacctgcca
     7321 gtcgtgcagg ttttaaacgg gcccacacgg ccagagcggg gtataaatga tccatttgga
     7381 actggggccg cggctttgcc gcggctcttt tttacgcctg ctccctcccc ggaaacatga
     7441 ataaaatgta aaagctgccc ccgcccagag gaaaacccgc ttctcctctt gaaagttggg
     7501 gtcatttctt atataatatt taagaaatgt gcggtcgtga accacgcgca ttgccaactg
     7561 aacgagagga ctgaacaaac tgtgagtgca tcccgagaga agaagtcccg ccaggacccg
     7621 agcggtcagg gtctcagtga aaagaagctc aagcagcagc aggaggggcg tcagacccat
     7681 gtccaccaca tcgtctacac ggtgcttgcc gttctggtgg tcctgttcgg cgtcggcctt
     7741 ctgatctgga acagcaattt cttccagagc cgcgccgccg ccgtcaccat cgatgggcag
     7801 gactacaccg ccgccgatgt gcagtactac tacaacggcg cccttcagca ggagatctac
     7861 aacagctaca gcggcgccag caccatggac tacaccctcg atcccgagga ccagatctac
     7921 gacgagtcca ccggcgagac ctggcgcgac catctgctgg acgtggccat ccagaccctg
     7981 actcagcagg cggccattgc caacgaggcc caggccagcg gatacaccat gtccgcccag
     8041 gcccaggaga gcctgcagaa caccctggac tccattcagg ccggcaccat cacctccggc
     8101 tacggcagca aggacgccta tgtccgcgcc aactacggcc ccaccatgag ctatgacaaa
     8161 ttcgtccaga tcatggagcg ctactatctg gccgctgact acgcccagag ccaggtggac
     8221 tcctacacct acgacgacag ccagctggat gcctactatg aggagcacgc cgacgagctg
     8281 gacaccttca ctctgtccca gttcgttttc caggcccggg tgaacaccgt ggacgacgag
     8341 ggcaacacca ttgaaatgac cgatgaggag aaggccgccg ccctggagga ggagaaggcc
     8401 gccgttaagg agcaggccga ggcgctccag gcccgcctgg aggccgggga ggacccggag
     8461 gccctggcgg aggagttcag tgactccatt tactcctccg aggtggcgct ggaacagatg
     8521 ggctccaccg tcaattcgga atacagcgag tgggcctatg acagcgcccg ccggcccggc
     8581 gacatcacct tggtggagta tgccggcggc agcagcgaga gctataacta ctgcgtagcc
     8641 ctcttccacg accgtgtacg ggatgacgcc cccaccgccg atgtgcgcca catcctgatc
     8701 ggcgccgccg acgccaacat cacccccacc gaagagcagt atgagcaggc aaaggccaag
     8761 gcggaggaac tgctggccca gtgggaggcc ggcgatgcca ctgaggactc ctttgcccag
     8821 ctggcccagg agaacagcgc tgacaccagc tctgcttcca acggcgggct gctccagtat
     8881 gtctctcctt actccggcta tgtggacacc tttacccagt ggtccctgga ccccagccgc
     8941 cagcccggag acaccggcat catccagaac accggcagtt ccgtcaaggg ttatcatatc
     9001 atgtactttg caggctggga cgaccccgtc tgggccgtca gcgccaaaaa cgccctgaac
     9061 aacgagcagt acagccagtg ggccgaaaaa ctggccagtg cctgcactgt cacccggggc
     9121 agcggtctga aatatgtgga gtgatcctcc caatcaatac gagcggcgcc cggtgcttca
     9181 agcaccgggc gccgtctctt tctctattct tccccatgct gaggcttttc gtcaaactcc
     9241 cggaaggcca gattcatgtc cacatttccc tcgatcccgg gcaccgaggc ggtgtgggtg
     9301 tactgccaca ggtcgaagtg gtagaagaag ctggggcggg cgttatactc cgccagccag
     9361 aaggtatagt cggtgagccg atccagctgg taggacagat aacccaggtc ctggttaaag
     9421 tagaccaccg ggtggtagcc cgcctcccgg atcatatcac agaaggcccc ggcgcagcgt
     9481 gtgatggcct ccggctccat gccgtccgtc cgggctgtgt ccccggcaat gaactcccag
     9541 tcaaaggcca cggggtaggt cacatcgtac ccctgaatgg cgtccaggac gtactgcgcc
     9601 tcctcctccg cctcccagac gctgacggcc tgggagaaaa agtagacccc cacctccagg
     9661 ccagcgtcca gcgccccccg gaggttctgc tcaaaattct tgtccggcat gatgaccccc
     9721 tgggagtatc cccggtaccc cagccggatc atggcaaatt ccactcccga atccgctacc
     9781 tgctgccagt cgatctcccc ctgataagcg gacacatcga tgccgatggc cgcctgcttt
     9841 ccatcctgct cataggtgag ccagccccga tcattgacgg agaagccgtc tgacacatag
     9901 ctgttcaccg ccaccccttc caggacgggg agctcccggt cccggtagga gatggtaggg
     9961 gactctgctc tctcccctgg ctcctgtggg tcatcctccc ccggcggc
//
LOCUS       DBMDSJ010000012         9853 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2244_length_9853_cov_1.787736, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000012 DBMDSJ010000000
VERSION     DBMDSJ010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2244_length_9853_cov_1.787736"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(67..831)
                     /locus_tag="ACZPAQ_00710"
     CDS             complement(67..831)
                     /locus_tag="ACZPAQ_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0902523.1"
                     /translation="MMELCHITAGYGGPPVLRDVSLAFEPGKVTVLAGPNGCGKSTLL
                     RVAARLMAPERGEVRIEGQDVSRLSAKQFARLAALLPQSRPLPGITAGSLVLHGRFPY
                     LGYPRRYSREDREAARQAMERTGVAGLEGVPMAHLSGGQRQKVYLAMALAQDTPVLLL
                     DEPTTFLDIAHQLELAETARTLAGEGKAVVMVLHDLNLALSCAHRVAVLEEGQLRRVG
                     APEEIYASGVLEKVFQVRVHSVETAKGERQYLFSRL"
     gene            complement(828..1838)
                     /locus_tag="ACZPAQ_00715"
     CDS             complement(828..1838)
                     /locus_tag="ACZPAQ_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR0902524.1"
                     /translation="MKSLPTSYTAARKWGIILLLALASLGAALLSMSFGSTQVPFSEI
                     LTALMGGETQAYNIVVYARLPRTLAALLCGSALAVGGVIIQGVLGNPLAGPNIIGVNA
                     GAGLGAVLCAALIPTSMTAVPVAAFLGALGACMLVYTLARRTGASRITLVLAGVAISS
                     MLSAAIDAVSILFPEAALGAGSFMVGRLSGVTVAALKVPALLSLLAGAGAFAMSYELD
                     LLTLGDEVAQSLGLSARAARNLLLTLAAVLCGAAVSFAGLVGFVGLLVPHAARFLVGG
                     EHKYLLAASLFLGAALVTVCDLAARVLFVPFELPVGILLSLMGGPFFLWLLFRQRGGR
                     RT"
     gene            complement(1801..2763)
                     /locus_tag="ACZPAQ_00720"
     CDS             complement(1801..2763)
                     /locus_tag="ACZPAQ_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0902525.1"
                     /translation="MKRSISLVTALCLLSLCACAAPGGRAVSPASADGEMGSSVSFTD
                     SLGSTITLEEAPQRVAALIGSYAETWVLAGGEDTLAAVTQDAYDERGLELGEDVTNVG
                     SNQTPELEALFAAQPDLVLLTPDLDGQLALAEPLAAAGIPAAWFKVESFSDYLSMLEI
                     CTRLTGRDDLYQANGLDIQAEIDRVLARVPEGEHPTVLLLRAYSTGVRAKNSDNITGV
                     MLKDLGCINIADSDSGLLEEIQMESILAADPDHIFVTTMGSSTEKALDSLTELFHSDP
                     AWESLAAVKEGRVHILDKALFHYKPNARWGESYEVLADILYGGA"
     gene            complement(3021..3920)
                     /locus_tag="ACZPAQ_00725"
     CDS             complement(3021..3920)
                     /locus_tag="ACZPAQ_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR0902526.1"
                     /translation="MVNVKPEQFQKIWSALITPTNEDESVNYEALERIVELQIQDGAE
                     GFYCCGSSGEGLLLTLEERKQVLEHVLKAADGRVPVISHVGTIRTRDVVDLARHAMSA
                     GALAVSMIPPYYYKFSMDEIMGYYEDVIRAIPDVPAIVYNIPQFTGVEFSKENAGRLL
                     SNENIVGIKHTSTNLYSLERMGQAFPGKALINGFDEQLLGALAMGSCATIGTTVNLFA
                     PLFHRVRDAFDRGDMAQAYRWQHAINLRVEETVKLGIFNAMKYGWTLRGVDCGFCRAP
                     FRPLDGAAREKMAQLMALPLEPV"
     gene            complement(3914..4747)
                     /locus_tag="ACZPAQ_00730"
     CDS             complement(3914..4747)
                     /locus_tag="ACZPAQ_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR0902527.1"
                     /translation="MSDQLFSVENKICIVTGGLGQIGKNFVQELWKRGARVAVWGRTV
                     NDERIAKTLGEEALKTSALRFYSVDITKKEEMNAALDDMEEVWGDAPDVLVNNAGIDT
                     QPSAPPEVSGPFENFPEEIFREVVDVNLVGTFLACQAVGARMVKAGKGGSIINVGSIY
                     GMLSPIQDIYAYKKEMTGVPFIKPVAYSAAKSGIYNLTRYLATYWGKQGIRVNTLTPA
                     GVWRDTQDEIFQANFCARMPMGRMSQENEYNGALIFLASQASSFMTGSNLVIDGGWTA
                     W"
     gene            complement(4800..6068)
                     /locus_tag="ACZPAQ_00735"
     CDS             complement(4800..6068)
                     /locus_tag="ACZPAQ_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR0902528.1"
                     /translation="MAIFLIAFVLLLVIGVPISISIGASAVLGCLSLGYPLVVIGQKM
                     VSGIDSFLLIAVPLFILAGNLMNAGKITEKIFDTAKELVGWIPGGLGHANVVASIIFA
                     GMSGSAVADAGGLGAIEMEAMKKNGYDEDFAGAVTAASSVIGPIFPPSIPLIIYGSVA
                     SVSVDQLFMGGVVPGLLMGVLLMVMVLYFAIVRRYERHPFRLRALIRQFLGSIPALIT
                     PVIILCGFVVGWFTPTEASSIAVIYSLLIALFLYRTLDWKSFKKCLKDSAISSANTLF
                     IIGTSTLFTYVMAMEGISRQFADVILGISSNPNVVLLVINVLLLVLGMVMEPGAILTL
                     MLPVLLPIANGLGLDLVHFGVMVVLNLMIGQVTPPFGVCLFVISDVNKLKLERLYRSI
                     LPFLVPLILTLILVTYIPGIVTALPNALLS"
     gene            complement(6084..6587)
                     /locus_tag="ACZPAQ_00740"
     CDS             complement(6084..6587)
                     /locus_tag="ACZPAQ_00740"
                     /inference="COORDINATES: protein motif:HMM:NF016200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR0902529.1"
                     /translation="MKLCNKISKGVAKGAEVICMLTIAALVLVIVVELLRRNFLGQSF
                     GGNVQLCGILFLWMAFIGLIPLYRDSGLMRLDFLVARTKGPVYEVLYFANKLFSLLLG
                     VVMVVAFAAQYPYVSTRTYSTFPIPVPYTVQYFPMCLAGAYMALESLRQMAEHVAEKA
                     RGSRAQV"
     gene            complement(6663..7733)
                     /locus_tag="ACZPAQ_00745"
     CDS             complement(6663..7733)
                     /locus_tag="ACZPAQ_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015445.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR0902530.1"
                     /translation="MKKLASLVLAGAMAFSLAACGNNSTPAGGSSANAGTSTPAASTP
                     ASGGIEPIELTIYSPGNENSVPTKTIIEYARLVNEASDGAITLDVHHSNELGSDAESI
                     ESTRMGTIDLIFAGTSGFTSFYEKAKVLDLPFLFDSAEQAYEVTNSEIGEQIFSGLEE
                     FGLVFLSEGDNGMRHISTTNRPVHTAADVQGLKIRVPESQLYLDVWQALGATPVALAL
                     PELALALSNGTAEAQDNATYHLVANATYDDIKYFSNINYMWMGCTMAANANTWNGLDP
                     AVQEILKEQAKAAAKYSFDTIAADNETATKTLQDAGVEFDFEPDVQSFKDALGGDAYY
                     DQYKDESWYDQELLDAILAEVR"
     gene            complement(8098..8811)
                     /locus_tag="ACZPAQ_00750"
     CDS             complement(8098..8811)
                     /locus_tag="ACZPAQ_00750"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR0902531.1"
                     /translation="MEPISRVPIVQQVEERIRELIQEEQYQPGKKLPPEMELCQRLGV
                     GRGTVREAFRLLQAKGVVEIKPGRGAFVAENPAERDIGAIDWLVENERELRDSIEIRT
                     ALEPMAARRMAERCNTEDIRRLENIHRSFLSAIQEGDAAMIGRLDEQFHSAIVESSGN
                     ALMIEINSHVCQGMQTFRSKTFQVEQNVKNAVMPHSNIMNAILARDAAQAEREMRAHL
                     DKVQEDLTQNIHAPGHETK"
     gene            9044..>9853
                     /locus_tag="ACZPAQ_00755"
     CDS             9044..>9853
                     /locus_tag="ACZPAQ_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902532.1"
                     /translation="MKPRAYYQPYQSGLLLAQAFSPSKTPLLRMEHLRQLDFPISREI
                     SYERTVDEFLLQLAVNDALRALRSRKDMVILLNEEGALIREDWHWSLLFTPNRSEKRT
                     LGDSSDLYQVLCSLAQRRQDGEVCRVAVPDRSLKTLVSGSDPFCILDEFCKSQPGGYL
                     AVAQNIVLEGTDHLFRHVPVCRYRVLSTVDLGEIENYHAIKTLLDEYIYAYDHRDAGE
                     DAPKPISIAVFGPPGSGKSFGVKQIALSRGRFRITSLNLSQYDSVPQLFHAL"
BASE COUNT         1853 a         3200 c         2981 g         1819 t
ORIGIN      
        1 cctatctcag ggggacggct ctttttatca tacaagaaaa cgggcagggt ggccccccct
       61 ccgctctcac agccggctga agaggtactg ccgctccccc ttcgccgtct ccacgctgtg
      121 gacccggacc tggaatacct tctccagcac gccggaggcg tagatctcct ccggcgcacc
      181 cacccgccgc agctgtccct cctccagcac cgccacccgg tgtgcgcagc tcagggccag
      241 gttcaggtca tggaggacca tgaccaccgc ttttccctcc cccgccaacg tccgggcggt
      301 ctccgccagc tccagctggt gggcgatatc cagaaaggtg gtgggctcgt ccagcagcag
      361 caccggggtg tcctgggcca gcgccatggc cagataaacc ttctgtctct gtccaccgga
      421 gaggtgggcc atgggcaccc cctccagccc ggccacaccg gtgcgctcca tggcttggcg
      481 ggccgcctcc cggtcctccc tggagtagcg gcggggatag cccagatagg ggaagcgccc
      541 gtggaggacc aggctccccg ccgtgatgcc gggcagcggc cggctctggg gaagcagcgc
      601 cgccaaccgg gcaaactgct tggcggagag ccgggacacg tcctgcccct ctatccttac
      661 ctctccccgt tccggtgcca tcagccgggc cgccacccgc aggagagtgg attttccaca
      721 gccattaggc ccggccagga cggtgacctt ccccggctca aaggccaggg atacgtccct
      781 cagtaccggc gggccgccgt agcccgccgt gatatggcat agctccatca tgtccgccgc
      841 cctcccctct gccggaacag cagccacaga aagaaggggc cgcccatcag ggagagcagg
      901 atgcccactg gcagctcaaa agggacgaac agcacccggg ccgccaggtc acacactgtc
      961 accagcgccg cccccaggaa cagggaggca gccagcaggt acttgtgttc cccccctacc
     1021 aggaagcggg ccgcgtgggg caccagcagg cccacgaagc ccaccagccc ggcaaagctc
     1081 accgccgcgc cgcacagcac cgccgccagg gtaagcagga ggttccgggc cgcccgggca
     1141 gacagcccca gggactgggc cacctcatcc cccagggtga gcaggtccag ctcatagctc
     1201 atggcaaagg cccccgcccc ggccaggagg gagagcaggg ccggcacctt cagggcggcc
     1261 actgtcaccc cagacagccg gcccaccata aagctgcctg cccccagggc ggcctccggg
     1321 aacaggatgg agaccgcgtc gatggcggcg gagagcatgc tgctgatggc caccccggcc
     1381 agcaccaggg tgatgcggct ggccccggtc ctccgggcca gggtgtacac cagcatacac
     1441 gcccccagcg cccccaggaa agctgccacc gggaccgccg tcatagaggt cggaatcagg
     1501 gcggcgcaga ggactgcccc caggcctgcg ccggcattca ccccgatgat attgggcccc
     1561 gccagggggt tgcccagcac tccctggatg atgaccccgc ccaccgccag ggcactgccg
     1621 cacagcaggg ccgccagggt gcggggcagg cgggcataga ccacgatatt gtatgcctgg
     1681 gtctcaccgc ccatcaaggc ggtcaggatc tctgaaaacg ggacttgggt ggagccgaag
     1741 ctcatgctca gcagagccgc ccccagactg gccagagcca gcaggaggat gatcccccac
     1801 ttacgcgccg ccgtatagga tgtcggcaag gacttcatag ctctctcccc atcgcgcatt
     1861 aggcttgtag tggaacaggg ccttgtccag gatatggacc cgcccctctt tcaccgccgc
     1921 aagggactcc caagccggat cgctgtggaa cagctccgtc aggctgtcca gcgccttctc
     1981 cgtgctggag cccatggtgg tcacgaagat atggtccggg tccgccgcca ggatggactc
     2041 catctggatc tcctccagca gtccgctgtc gctgtccgcg atgttgatac agcccagatc
     2101 cttcagcatc acgccggtga tattgtcgct gttcttggcc cggacgccgg tggagtaggc
     2161 ccgcagcagc aggaccgtgg ggtgctcccc ctccgggaca cgggcaagca cccggtcgat
     2221 ctctgcctgg atatccagcc cgttggcctg atacaggtcg tcccgccccg tcagacgggt
     2281 gcagatctcc agcatggaga ggtagtcgga gaaggactcc accttgaacc aagcggcggg
     2341 aatgccggcc gcagccaggg gctccgccag cgccagttgg ccgtccaaat cgggagtcag
     2401 gagcaccagg tcaggctggg cagcgaaaag cgcctccagc tccggggtct ggttggagcc
     2461 cacgttggtc acatcctccc ccagctccag cccccgctca tcgtaggcgt cctgggtcac
     2521 cgctgccagc gtgtcctccc ctcctgccag gacccaggtc tccgcgtagc tgccgatcag
     2581 ggctgccacc cgctggggcg cttcctccag ggtaatggta ctgcccaagc tgtccgtgaa
     2641 gctcacagag ctccccatct ccccgtccgc cgacgcgggg gacaccgccc ttccgccagg
     2701 agcggcgcag gcgcagaggg acagcagaca gagtgcggtg accagggata tggatcgttt
     2761 catcatcatc tgggatttcc tttcttttca gaagcctggg tccggagccc cagaccgaat
     2821 gaccggcccc attttaccac aagatctccc cggatgccac aaaaatcttt tctttccacg
     2881 gcctgaaagg acttttctcc cccatgctcc cgggatgcgg gtccggctgc ctcaatgctg
     2941 tgcgcccgcc gcggcctgga gccgcggcgg gcgctgtctc tctcttttcg atcaggatac
     3001 gaatctgacg ggccgggagc tcataccggc tccaggggca gggccatcag ctgggccatc
     3061 ttctccctgg ccgctccgtc caggggccgg aagggggcgc ggcagaagcc gcagtccacc
     3121 ccccgcaggg tccagccgta tttcatggcg ttgaaaatgc ccagcttcac cgtctcctcc
     3181 acccggagat tgatggcgtg ctgccagcgg taggcctggg ccatgtcccc ccggtcaaag
     3241 gcgtcccgca cccggtggaa caggggggca aacaggttga ccgtggtacc gatggtggcg
     3301 caggagccca tagccagagc tcccagcagc tgctcgtcaa agccgttgat cagggccttg
     3361 ccagggaatg cctgccccat ccgctccagg ctgtacaggt tggtggaggt gtgcttgatg
     3421 cctacgatgt tctcgttgga cagcagccgt cccgcgtttt ccttgctgaa ctccactccg
     3481 gtgaactggg gaatgttata gacgatggcg ggcacatcgg ggatggcccg gatcacgtcc
     3541 tcgtagtagc ccatgatctc gtccatggaa aacttatagt agtagggcgg gatcatggac
     3601 accgccaggg ccccggcgga catggcgtgc cgcgccaggt ccaccacatc ccgggtgcgg
     3661 atggtgccta catgggagat aacgggcacc cgcccgtccg ccgccttgag cacgtgctcc
     3721 agcacctgct tgcgctcctc cagggtgagc agcagcccct cccccgagga cccgcagcag
     3781 tagaagccct ccgcgccgtc ctggatctgg agctccacga tgcgctccag ggcctcataa
     3841 ttgacgctct cgtcctcgtt ggtgggggtg atgagggccg accagatctt ttggaactgc
     3901 tccggcttca cgtttaccat gctgtccagc ccccgtcgat caccagattg gaacccgtca
     3961 taaaggagga cgcctgggag gccaggaaga tcagggcgcc gttgtactca ttctcctgag
     4021 acatgcggcc catgggcatc cgggcgcaga agttggcctg gaatatctcg tcctgggtgt
     4081 cccgccacac gccggcgggg gtgagggtgt tgacccggat gccctgcttg ccccagtagg
     4141 tggccaggta gcgggtcagg ttatagatgc cggacttggc ggcagagtag gccaccggct
     4201 tgatgaaggg gacgccggtc atctccttct tataggcata gatgtcctgg atgggagaga
     4261 gcatgccata gatggagccc acattgatga tggagccgcc cttgcccgcc ttgaccatcc
     4321 gggcgcccac cgcctggcag gccaggaagg tgcctaccag gttcacatcc actacttccc
     4381 ggaaaatctc ctccgggaag ttctcaaagg gaccggacac ctccggaggc gcagagggct
     4441 gagtgtcgat gccggcgttg ttcaccagca cgtcaggggc gtccccccag acctcttcca
     4501 tgtcgtcaag ggcggcgttc atctcctcct tcttagtgat gtccacagag taaaaacgca
     4561 gagcggaggt cttcagggcc tcctccccca gggtcttggc gatgcgctcg tcgttgacgg
     4621 tgcggcccca cacggccacc ctggcgcccc gcttccagag ctcctggacg aaattcttgc
     4681 cgatctgccc caggccgccg gtgacgatgc agatcttatt ttctacgctg aacagttgat
     4741 cgctcatgat gtgatgtact cctctctaac aattggatga tttggaacag gggacgttct
     4801 cagctcagca gggcgtttgg cagggctgta acgatccccg gtatataggt taccagaatc
     4861 agcgttaaaa tcaaggggac caggaagggc aggatggatc gatacagccg ttccagcttc
     4921 agcttgttta cgtcggagat gacgaacagg cacacgccga agggcggggt cacctgaccg
     4981 atcatcaggt tgagcaccac catgacgccg aagtggacca ggtccagccc caggccgttg
     5041 gcaatgggca gcagcacagg gagcatcagg gtcaggatgg cgccgggctc catcaccatg
     5101 cccaggacga gcagcaggac gttgatgacc agcaggacca cgttgggatt gctgctgatg
     5161 cccaggatca catcagcgaa ctggcgggag atgccctcca ttgccatgac gtaggtgaac
     5221 agggtagagg tgccgatgat gaacagggtg ttggcagagg agatggcgga gtccttcagg
     5281 cacttcttga agctcttcca gtccagggtc cggtagagga agagggcgat cagcagggag
     5341 tagataaccg caatggaaga cgcctctgta ggggtgaacc agcccaccac gaagccacac
     5401 aggatgatga ccggggtgat gagggcgggg atggatccca ggaactggcg gatcagggcc
     5461 cgcaggcgga aggggtggcg ctcatacctg cgcacgatgg cgaagtaaag gaccatcacc
     5521 atgagcagta cgcccatcag caggccgggc accacgccgc ccatgaacag ctggtccaca
     5581 ctgacgctgg ccacacttcc gtagatgatc agggggatgg agggcgggaa gatgggtccg
     5641 atgacggagg aggctgcggt gacggcgccg gcgaagtcct cgtcatagcc gttctttttc
     5701 atggcctcca tctcaatggc gcccaagccg ccggcgtcgg ccacagcgga gccggacatg
     5761 ccggcgaaaa tgatgctggc caccacgttg gcatggccca ggccgccggg gatccagccc
     5821 accagctctt tggccgtatc aaagatcttc tccgtgatct tgcctgcatt catcaggttg
     5881 cccgccagaa tgaacagggg cactgcgatc agcaggaaag agtcgatgcc gctgaccatc
     5941 ttctggccga tgaccaccaa gggatacccc agagacaggc accccagcac ggcggaggcg
     6001 ccgatggaga tgctgatagg gacaccaatg accagaagga gcacaaacgc tatcagaaaa
     6061 attgccatat ccgtgtccct cccttatacc tgcgcccggc tgccccgggc cttttctgcc
     6121 acgtgctccg ccatctgccg cagagactcc agcgccatat aggctccagc caggcacatg
     6181 gggaaatact gcaccgtata gggcaccggg atagggaagg tggaataggt gcgggtgctg
     6241 acatagggat actgggcggc gaaggcgacg accatcacca cgcccagcaa caggctgaac
     6301 agcttgttgg cgaagtacag cacctcgtac accgggccct tggtgcgggc gacaaggaag
     6361 tccaggcgca tcaggccgct gtcccggtac agggggatca ggccgataaa ggccatccac
     6421 aggaagagga tgccgcacag ctgcacgttc ccgccaaagg actggcccag gaagttacgg
     6481 cggagcagct ccaccacgat gaccagcacc agggccgcaa tggtgagcat gcagatcact
     6541 tccgcgccct tggccacccc tttggagatc ttgttacaca atttcatacg atttctcccc
     6601 tctacaaagg acagctgcat aggcaggggc ctatgcagct gtcagcgcag attttgtttg
     6661 gatcagcgca cctccgccag gatggcgtcc agcagctcct ggtcgtacca gctctcatcc
     6721 ttatactgat catagtaggc gtcgccgccc agcgcgtcct tgaaggactg gacatcaggc
     6781 tcgaagtcga actccacgcc ggcgtcctgc agagtcttgg tggcggtctc gttgtcggcg
     6841 gcgatggtgt caaagctgta cttggcagct gccttggcct gttccttcag gatctcctgt
     6901 acggcggggt ccaggccgtt ccaggtgttg gcgttggcgg ccatggtgca gcccatccac
     6961 atgtagttga tgttggagaa gtatttgatg tcgtcataag tggcgttggc gaccaggtga
     7021 taggtggcat tatcctgggc ctcggcggtg ccattggaca gggccagggc cagctcaggc
     7081 agagccaggg ccacgggggt ggcgcccaga gcctgccaca cgtccaggta cagctggctc
     7141 tcaggcacgc ggatcttcag gccctgcacg tcggcggcgg tgtggacggg ccggttggtg
     7201 gtggagatgt ggcgcatgcc gttatcgccc tcggacagga agaccaggcc gaactcctcg
     7261 aggccggaga agatctgctc accgatctcg ctgttggtca cctcataagc ctgctccgcg
     7321 ctgtcaaaca ggaagggcag gtccagcacc ttggctttct catagaagga ggtaaaaccg
     7381 gaggtgccgg caaagatcag gtcgatggtg cccatgcggg tggactcgat agattcggcg
     7441 tcgctgccca gctcgttgga gtggtggaca tccagggtga tggcgccgtc agaggcctcg
     7501 ttcaccagac gggcgtactc aatgattgtc ttagtgggga cggagttctc gttgccgggg
     7561 gaatagatgg tcagctcaat gggctcgatg ccgccggagg cgggagtgct ggcggctgga
     7621 gtgctggtgc cggcattggc ggagctgccg ccggcaggag tgctgttgtt tccgcaggca
     7681 gccagggaaa aggccatggc gccggccagc acaagggaag caagtttttt cattacagga
     7741 tcctccttat cgttgtgtcc gaagctacat accaccgtcc tccctttcgt cagacggacg
     7801 attgtatgac atctgactat agtatattaa gactgcatta agatgtcaat ggaaaaagcc
     7861 gacaaaattc ctctctcatt tttgtgctaa tttccgctct gctctctctt tttctcaaat
     7921 ttctcaacat attgtacaaa gccggttttc ccctggaccc cgcccgtctt cctggcacgt
     7981 ccaaaacacc gctgcctgag ccggaccgtg tcctcttctc tgccgggaca cagaagaaat
     8041 cccccagccg caccggtacg gctgggggtc tcaaactgcc gggctgggca gttccgatta
     8101 ttttgtctcg tgtcccgggg catggatgtt ctgggtcaga tcctcctgga ccttgtccag
     8161 atgggcccgc atctcccgct ccgcctgggc ggcatcccgg gccaggatgg cgttcataat
     8221 attgctgtgg ggcatgaccg cgttcttcac gttctgctcc acctggaagg tcttgctgcg
     8281 gaaggtctgc attccctggc acacatggct gttgatctcg atcatcagtg cgttcccgct
     8341 gctctccacg atggcgctgt ggaactgctc gtccaggcgg ccgatcatag ccgcgtcccc
     8401 ctcctgaatg gcgcttagga agctacggtg gatgttctcc aggcggcgga tatcctcggt
     8461 gttgcatcgc tccgccatcc gccgggccgc cataggctcc agcgccgtgc ggatctcgat
     8521 ggagtcccgc agttcccgct cgttctccac cagccagtcg atggccccga tatcccgctc
     8581 tgcagggttc tccgccacaa aggccccccg tcccggcttg atctccacca cacccttggc
     8641 ctggagcagc cggaatgcct cccgcacagt gccacggccc acccccaggc gctggcacag
     8701 ctccatctcc ggcggcagct ttttcccagg ctgatactgc tcctcctgga tcagctctct
     8761 gatcctctcc tctacctgct ggacaatggg gaccctggaa atgggttcca tatgcctcac
     8821 ctccgcctgt cttttgggca tgtcattgtt tttattctac aacccgatgc cctatacgtc
     8881 aaggatttct ttcttttgta tgacaattat tgttctttgt atgacatttt tctcttttca
     8941 tctcccatcc ccagctcttg ccaaggccgg aaaaagcgtc tacaatagag aaaagacacc
     9001 tgccgccggg tcccggtccg gcccgctcag aaaggagccc gccatgaaac ccagagccta
     9061 ctatcagccc tatcagtccg gcctgctgct ggcccaggcc ttctccccct ccaagacgcc
     9121 actgctccgc atggagcacc tgcgccagct ggactttccc atcagccggg agatctccta
     9181 cgagcgcacc gtggatgaat tcctcctcca gctggcggta aatgatgccc tgcgggccct
     9241 gcgcagccgg aaggacatgg tgatcctcct caacgaggag ggcgccctca tccgggagga
     9301 ctggcactgg tccctgctct tcacccccaa ccgcagcgag aagcggaccc tgggtgactc
     9361 ctccgacctc tatcaggtcc tgtgcagcct ggctcagcgc cggcaggacg gtgaggtctg
     9421 ccgggtggcg gtgcccgacc ggagcctgaa gaccctggtc tccggttccg accccttctg
     9481 tatcctggac gagttctgca aatctcagcc cgggggctat ctggcagtgg cccagaacat
     9541 cgtgctggag gggacggacc acctgttccg ccatgtcccc gtctgccgct accgggttct
     9601 gtctacggta gacctggggg agatcgagaa ctaccacgcc atcaagaccc tgctggacga
     9661 gtacatctat gcatatgacc accgggacgc aggggaggac gcccccaagc caatctccat
     9721 cgccgtcttc ggacctcctg gctccggcaa gtccttcggc gtcaagcaga tcgccctgag
     9781 ccggggcagg ttccgcatca cctccctgaa cctgtcccag tacgactcgg tcccccagct
     9841 cttccacgcc ctc
//
LOCUS       DBMDSJ010000013         9722 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2287_length_9722_cov_1.606694, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000013 DBMDSJ010000000
VERSION     DBMDSJ010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_9722_cov_1.606694"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="ACZPAQ_00760"
     CDS             complement(<1..1540)
                     /locus_tag="ACZPAQ_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HIR0902533.1"
                     /translation="MENQAWKGFHGGEWQRSIDVRSFIQKNWTPYEGDGSFLTGPTRR
                     TRAVWERCEDLLRQELRKGVLDVETHIVSGIDSFAPGYIDRENEVIVGLQTDAPLKRI
                     VNLYGGTRMARSALEQYGYRLDPEIEDHFSQYRKTHNEGVFDAYPERTRKARHAGLLT
                     GLPDAYGRGRIIGDYRRVALYGIDRLVEEKGRDLDALDGPMDEERIRLREEVSEQIRA
                     LEEMKKMAARYGVDLSRPSATAREAVQAVYLAYLAGIKENNGAATSLGRTSTFLDIYL
                     QRDLEAGVITEEEAQELIDQFIIKLRLVRHLRTPEYNELFGGDPTWVTEAIGGMSIDG
                     RTLVTRTSFRYLHTLGNLGSAPEPNLTVLWSEHLPAPFKAFCAKMSILTDSIQYENDD
                     VMRPVYGDDYAISCCVSAMAVGKQMQFFGARCNIAKCLLYAINGGVDERTGETVVPGI
                     RPILDEVLSYEAVRANYEQVLSYAAELYADTVNIIHYMHDKYAYEASQMALHDTQVER
                     LTAFG"
     gene            complement(1543..2280)
                     /gene="pflA"
                     /locus_tag="ACZPAQ_00765"
     CDS             complement(1543..2280)
                     /gene="pflA"
                     /locus_tag="ACZPAQ_00765"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR0902534.1"
                     /translation="MTGYIHSMESMGLVDGPGVRAVVFLQGCPLRCRYCHNPDTQCRG
                     GRPVEDGTLVRRLLRFRSYFDRSGGGVTFSGGEPLAQPDFLLACLRRLKAEGVHTCLD
                     TSGAGRGIYGEILAHTDLVLYDVKHHEPAGYKAVTGGDMAATLDFVEAVRRAGTPMWV
                     RHVVVPGLTDSRAHLEGLRAYVDTLPHVERVELLPFHKLGAHKYEALERTDPLAGTPP
                     MDRDLCRRLEREFFAPYTTTAEKGRAV"
     regulatory      complement(2351..2431)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(2544..3974)
                     /locus_tag="ACZPAQ_00770"
     CDS             complement(2544..3974)
                     /locus_tag="ACZPAQ_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR0902535.1"
                     /translation="MESLEATLYPIVVNINNYLSSYILVFLLIAVGLWYSIRTRFVQV
                     RCFGEGMRKVFGNLTLRGKKHASGMSSFQALATAIAAQVGTGNIVGSAGAILTGGPGA
                     IFWMWIIAFFGMATIYAEATLAIKTRKVDQEGNIHGGPVYYITTAFQGAFGKFLAGFF
                     AVAITLALGFMGCMVQSNSIGSTFQTAFGVPSWVVGIVLVVICAIIFLGGVQRLASVT
                     EKIVPVMAAIFLLGGLVVLIARIQYLPATIAMIFQYAFQPQAIIGGGFGAALKTAISQ
                     GAKRGLFSNEAGMGSTPHAHAQANVTDPHDQGVVAMVGVFIDTFVVLTLNALVIISTL
                     YTADGPLAGGYVGGVTEVLDKTNLAQTAFGTVMGAGLGAKFVAICLFFFAFSTVLGWN
                     LFGKINVIYLFGKKSPKLCTTIYTVVALIFIFLGTTMSNDLVWELTDMFNNLMVIPNA
                     IALFALTSVVVRVARREDPDSTPLRR"
     regulatory      complement(4096..4192)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(4193..4278)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4531..7305
                     /gene="secA"
                     /locus_tag="ACZPAQ_00775"
     CDS             4531..7305
                     /gene="secA"
                     /locus_tag="ACZPAQ_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR0902536.1"
                     /translation="MSLFTKIFGTSSQREVKAITPLVDKIESLEEEYKALTDQQLQAK
                     TPEFKERLQNGETLDDILPEAFAACREAAWRVLGMRPYRVQLIGGIILHQGRIAEMKT
                     GEGKTLVATLPAYLNALAGKGVHIVTVNDYLAKRDSEWMGKVYRFMGLTVGLVIHGVM
                     GPAKKAAYQADITYGTNNEFGFDYLRDNMAIYSSELVQRGHSFAIVDEVDSILIDEAR
                     TPLIISGQGEKSTQLYTIVDQFVSRLKCQRIAKVDNKEEEDVNLDADYVVDEKARTAT
                     LTARGIAKAEQAFNIENLADPENTTLSHHINQAIKAHGVMKRDIDYVVKDGQVIIVDE
                     FTGRLMFGRRYNEGLHQAIEAKEHVEVANESKTLATITFQNYFRLYDKLSGMTGTAMT
                     EQEEFGTIYELDIVEIPTNKPLARIDRPDVVYKTEAGKLRAIVQQIEECHQKGQPVLV
                     GTVSIEKSEHLSEMLRRKGIAHSVLNAKNHEKEAEIVAQAGKFGAVTVATNMAGRGTD
                     IMLGGNAEFLAKADLRKAGMTDELIAEATGFAETDNEDILNARKMFTEAEAKYKDEIR
                     AEADKVRTVGGLFILGTERHESRRIDNQLRGRAGRQGDPGETRFFLSLEDDIMRLFGS
                     ERVMGMMEKLGVDEDTPIDAKMLSNAIENAQKQVESRNFQTRKNVLQYDDVMNTQREV
                     IYKQRRQVLDGEDLQQSIQSMLRSTIENAIRGHMGEQKHMTAEEFREATALFHTMFLL
                     PDELRLTDEELQGYTADGLVDLVMEKAQAVYQRKEQEFTPPLMRELERVIMLRVVDEY
                     WMDQIDAMNDLKQGIGLRAYAQTDPVVAYKKEGYEMFEQMIAAIQEETLRRLFLVRIQ
                     RKAPDAPAPEIKRERVAKITSESGASDGTVKKQPVKKAVKIGRNDPCPCGSGLKWKKC
                     TCKEYHSEQQ"
     gene            7420..7788
                     /locus_tag="ACZPAQ_00780"
     CDS             7420..7788
                     /locus_tag="ACZPAQ_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024388.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HIR0902537.1"
                     /translation="MEQTDLIRRARYGPNTILAAGMSRALNTALNSAESELLLLVLIL
                     DRDSGRILDAECNIILGINSDYIASLLVGRNFYQELDEIILSIQQQYFGPGQRALIVC
                     LKDAYSKMRARSPRSLPLRK"
     gene            7953..8288
                     /locus_tag="ACZPAQ_00785"
     CDS             7953..8288
                     /locus_tag="ACZPAQ_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024388.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HIR0902538.1"
                     /translation="MSDFPSGAICVVGLAKVANQNPIHSVHRALAGSFIVDPSSGLVY
                     DVQFDTVCQITSDFLASLLIGLSLHTDLEEMISRVQSRYFGDSRRAITVILRDVAARL
                     RECQGGGYN"
     gene            8556..>9722
                     /locus_tag="ACZPAQ_00790"
     CDS             8556..>9722
                     /locus_tag="ACZPAQ_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014500.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntT/GntP/DsdX family permease"
                     /protein_id="HIR0902539.1"
                     /translation="MIFNLPPICGLFPLLLYIVLAFKKGMHPLVNVSICAVVGAIMVK
                     QPLNGLGAVIYESMGSFLALVGFIIMLGSGLGMVLRKTGVAAAIVNVLMRKIGVRTQK
                     RAMIATMVCSVVLVTLLGTLAGANAVIAPIVIPLVAAVGITPSALAVIFQGAGQTGLF
                     LSPFSPPMVTLMSITGLSYPRLLLCAGLPVSIVMWVGTFLIANKVQKETQGTENFGPD
                     GEAAAENFQMDPATRRATIAFAVTMALMVIYGIATNGGASFVILVMIVAAIITGLFAR
                     MPVGTIFDSMMEGCGKMMWLFFMFLIFNPFLNFVTQSGAFDALLQLLEPLIGPTGKVG
                     FTLLTVLVGIFGINGAAVAQAMMIDSLFSSFLPTLGISMELWGMIVLIGHQITSF"
BASE COUNT         1928 a         2984 c         2973 g         1837 t
ORIGIN      
        1 tgccgaaggc ggtgagccgc tccacctgag tgtcatggag ggccatctgg ctggcttcat
       61 aggcgtattt gtcgtgcatg tagtggatga tgttcaccgt gtccgcatac agctccgcgg
      121 cataggagag cacctgctca tagttggccc ggaccgcttc ataggagagc acctcgtcca
      181 ggatgggacg gatgccgggg accaccgtct caccggtgcg ctcgtccacg ccgccgttga
      241 tggcgtacag caggcatttg gcgatgttgc accgggcgcc gaagaactgc atctgtttgc
      301 ccaccgccat ggcggacaca cagcaggaga tggcgtagtc atccccatac accgggcgca
      361 tcacgtcgtc attttcgtat tggatggagt cggtgaggat ggacattttg gcacagaagg
      421 ccttgaaggg ggcggggagg tgctccgacc acagcacggt gaggttgggc tccggggcgc
      481 tgcccaggtt gcccagggtg tggagatagc ggaaggaggt tcgggtcacc agggtgcgcc
      541 cgtcaataga catgccgccg atggcctcgg tgacccaggt ggggtccccg ccgaagagct
      601 cgttgtactc cggcgtacgc aggtggcgga ccagccgcag cttgatgatg aactggtcga
      661 tcagctcctg ggcctcctcc tcggtgatga cccctgcctc caggtcccgc tggaggtaga
      721 tgtccaggaa ggtggaggtg cggcccaggg aggtggcggc cccgttgttc tccttgatcc
      781 ctgccagata ggccaggtag accgcctgca ccgcctcccg ggcagtggcg gaggggcggg
      841 acaggtccac cccataccgt gcggccatct ttttcatctc ctccagggcc cggatctgct
      901 ccgagacctc ctcccgcagg cggatgcgct cctcgtccat ggggccgtcc agggcgtcca
      961 ggtcccgtcc tttttcctcc accaaccggt cgatgccgta cagggcaacc cggcggtagt
     1021 ccccgatgat cctcccccgg ccgtaggcgt ccggcaggcc ggtgagcagg ccggcgtggc
     1081 gggccttccg ggtgcgctcc gggtaagcgt caaagacccc ctcgttgtgg gtcttgcggt
     1141 actgggagaa gtggtcctcg atctccgggt ccaggcgata gccgtactgc tccagggcgg
     1201 accgggccat ccgggttccg ccgtacaggt tgacgatgcg cttgaggggc gcgtcggtct
     1261 gaaggcccac gatgacctcg ttctcccggt cgatatagcc gggggcaaag ctgtcgatgc
     1321 cggagacgat gtgggtctcc acatccagca cgcccttccg cagctcctgg cgcagcaggt
     1381 cctcacaccg ctcccacacc gcccgggtgc gccgtgtggg gccggtaaga aagctgccgt
     1441 ccccctcgta gggggtccag ttcttctgaa taaaggagcg cacatcgatg ctgcgctgcc
     1501 actcgccgcc atgaaagccc ttccatgcct gattttccat cttcataccg ctcttccttt
     1561 ctccgcagtt gtggtgtagg gagcaaaaaa ttcccgctcc agccgccggc acagatcccg
     1621 gtccattggg ggtgtacctg ccagagggtc ggtccgttcc agagcctcat acttgtgggc
     1681 ccccagcttg tggaagggca gcagctctac ccgctctaca tggggcaggg tgtccacata
     1741 ggcccgcagt ccctccaggt gggcccggct gtcggtgagg ccggggacca ccacgtggcg
     1801 gacccacatg ggggtgcccg cccggcgcac tgcttctaca aagtccaggg tggcggccat
     1861 gtccccgccg gtgactgcct tgtagcccgc cggctcatgg tgcttcacat catagagcac
     1921 caggtcggtg tgggccagga tctccccata aattcccctt ccggctcccg aggtgtccag
     1981 acaggtgtgg acaccctccg ccttcagccg cctgaggcac gccagcagaa agtccggctg
     2041 ggccagaggc tcgccgccgg agaaggtcac gcccccgccg gagcggtcaa agtaactgcg
     2101 gaagcgcagc agcctgcgga ccagcgtgcc gtcctccacc ggccggccgc ccctgcactg
     2161 ggtgtcgggg ttgtgacagt agcggcaccg cagggggcag ccctggagaa agaccactgc
     2221 gcgcacccct ggaccgtcca ccagccccat gctctccatg gagtggatat atcccgtcat
     2281 cctgtttcct ccttgctttt ctgtgatggc ggggcaaaaa agggcgcaaa aaagccgccc
     2341 ctccgggctc cttcagcgcc cagacggtcg gcatggtgac gatcatactc cctgtggttt
     2401 cactccactt ccgtcagtca tatgatccac atccacaata cagcagagac ccgcctcctg
     2461 tcaagaggcg ggtccccgtt tctacttttt ccacaaatca gctgggccga gggaaggagt
     2521 tcctgtatct tgcggccgcc ggctcaccgg cgcagggggg tgctgtccgg gtcctcccgc
     2581 cgggccaccc tgactaccac gctggtcagg gcaaacaggg cgatggcgtt gggaatgacc
     2641 atcaggttgt tgaacatatc ggtcagctcc cacaccaggt cgttggacat cgtggtgccc
     2701 aggaaaatga agatgagggc gaccacggta taaatagtgg tgcacagctt gggggacttc
     2761 tttccaaaca ggtagatcac gttgatctta ccaaacaggt tccaccccag cacggtggag
     2821 aaggcgaaga aaaacaggca gatggccacg aacttggcgc ccagcccggc ccccatcaca
     2881 gtgccgaagg cggtctgcgc caggttggtc ttgtccagca cctcggtcac cccgcccacg
     2941 tagccgccag ccagagggcc gtcggcggtg taaagggtgg agatgatgac cagtgcgttc
     3001 agggtgagga ccacgaaggt gtcgatgaac acgccgacca tggccaccac gccctgatcg
     3061 tgggggtcgg tcacattggc ctgggcgtgg gcgtgggggg tggagcccat gccggcctcg
     3121 ttggagaaca ggccccgctt ggcgccctgg ctgatagcgg tcttcagggc ggcgccgaag
     3181 ccgccgccga tgatggcctg gggctggaag gcgtactgga agatcatggc aatggtggca
     3241 gggaggtact ggatacgggc gataagcacc accaggccgc ccagcaggaa gatggccgcc
     3301 atgacaggga cgatcttctc cgtgacagag gccagacgct gtacgccgcc caggaagatg
     3361 atggcgcaga tgaccaccag cacgatgccc accacccagg atgggacgcc gaaagcggtc
     3421 tggaatgtgg agccgatgga gttggactgg accatgcagc ccatgaagcc cagggccagg
     3481 gtgatggcca cggcgaagaa accagccagg aacttgccga aagcgccctg gaaggcggtg
     3541 gtgatgtagt acaccggtcc gccgtgaata ttgccctcct ggtctacctt gcgggtcttg
     3601 atggccagag tggcctcagc gtagatggtg gccatgccga agaaggcgat gatccacatc
     3661 cagaagatgg cccccggtcc gccggtgagg atggctccgg cggagcccac gatgttgccg
     3721 gtgcccacct gagcggcgat ggcggtggcc agggcctgga aggagctcat gccgctggca
     3781 tgctttttgc cccgcagggt caggttgccg aataccttcc gcatgccctc gccgaagcag
     3841 cgcacctgaa cgaagcgggt ccggatggag taccacaggc ccaccgcaat gagcaggaag
     3901 accaggatgt agctggacag gtagttgttg atgtttacaa cgatgggata gagcgtggct
     3961 tctaaggatt ccaacatagt gttttctctc tcctttggga caaaaaacgg accggggagc
     4021 aggctccgcg gtccgtcgca tagggcgagg aaaaactccc tcgccgcggt ccgttcctgg
     4081 actgcggcag ggcagttcat cgtctctgtc cttttgcctg agagattggc ggaagcgtcc
     4141 ccgcttccgt cttgcacctt cggcacccga tcgtcgagct tctccagagt cacatccacg
     4201 cgcagtcctc gtcccgacag ggacacctga gagtgttact ccttcggcaa gccatagctg
     4261 ttttcctgcg cgcttcgtct gtcctatttt gttttcgagc aataccttaa cacagccgcg
     4321 gtcaaatgtc aaccactggc gtacatttat acatatccgg cggatttccc cccggcactc
     4381 ccccggatat tgggcatttt cgcagacaaa gtttacagtt tggccttttt tccgggagaa
     4441 aggtatgata tactgagatg ctacgatcca tcaggacgcc ctttttccac aatgatcgag
     4501 gatcaacatc aacatccgag gtgttttcca atgagtctat ttactaaaat cttcgggacc
     4561 tcctcccagc gcgaagtcaa ggccatcacg ccgctggtgg ataagatcga atctctggag
     4621 gaggagtaca aggccctgac agaccagcag ctgcaggcca agacccccga atttaaggag
     4681 cgtctccaga acggtgagac gttggacgat atcctgcccg aggcctttgc tgcctgccgg
     4741 gaggcggcct ggcgggtgct gggcatgcgc ccctaccggg tccagctcat cggcggcatc
     4801 atccttcacc agggccgcat cgctgagatg aagaccggtg aaggcaagac cctggtagcc
     4861 accctgcccg cttacctcaa cgccctggcc ggaaagggcg tccacatcgt caccgtcaac
     4921 gactacctgg caaagcgcga cagcgagtgg atgggcaagg tgtaccgctt catgggcctg
     4981 accgtgggcc tggtcatcca cggggtcatg ggcccggcca agaaggccgc ctatcaggcg
     5041 gatatcacct acggcaccaa caacgagttc ggcttcgact acctgcggga caacatggcc
     5101 atctactcca gcgagctggt ccagcgcggc cactcctttg ccatcgtgga cgaggtggac
     5161 tccatcctca tcgacgaggc ccgcaccccc cttatcatct ccggccaggg ggagaagtcc
     5221 acccagctct acaccatcgt ggaccagttc gtctcccgcc tgaagtgcca gcgcatcgcc
     5281 aaggtggaca acaaggagga agaggacgtc aacctggacg ctgactacgt ggtggacgag
     5341 aaggcccgca ccgccaccct taccgcccgg ggcatcgcca aggctgagca ggccttcaac
     5401 atcgagaacc tggccgaccc ggagaacacc accctctccc accacatcaa ccaggccatc
     5461 aaggcccacg gcgtgatgaa gagggacatt gactacgtgg tgaaggacgg acaggtcatc
     5521 atcgtggacg agttcaccgg gcgcctcatg ttcggccgcc ggtataacga ggggctccac
     5581 caggctatcg aggccaagga gcacgtggag gttgccaacg agtccaagac cctggctacc
     5641 atcaccttcc agaactattt ccgcctctat gacaagctct ccggtatgac gggcaccgcc
     5701 atgaccgagc aggaggaatt cggcactatc tatgagctgg acattgtaga gatccccacc
     5761 aacaaacccc tggcccggat tgaccggcct gacgtagtct acaagaccga ggcgggcaag
     5821 ctgcgggcca ttgtgcagca gatcgaggag tgccatcaga agggccagcc cgtcctggtg
     5881 ggcacggtgt ccatcgagaa gtcggagcac ctgtccgaga tgctccggcg caagggcatc
     5941 gcccacagcg tcctgaacgc caagaaccac gagaaggagg ccgagatcgt cgcccaggcg
     6001 ggcaagttcg gcgccgtcac cgtggccacc aacatggccg gccgcggcac cgacatcatg
     6061 ctgggcggca acgccgagtt cctcgctaag gctgacctgc gcaaagcggg catgaccgac
     6121 gagcttatcg cggaggccac cggattcgcg gaaacggaca acgaggatat cctcaacgcc
     6181 cggaagatgt tcaccgaggc ggaggccaag tataaagacg agatccgggc ggaggcggac
     6241 aaggtccgca ctgtaggcgg cctgttcatc ctgggcaccg agcgccacga gtcccgccgc
     6301 atcgacaacc agctgcgggg ccgcgccggc cgtcagggcg accctggtga gacccgcttc
     6361 ttcctctcac tggaggatga catcatgcgc ctgttcggct ctgagcgagt catgggcatg
     6421 atggagaagc tgggggtgga cgaggacacc cccatcgacg ccaaaatgct gtccaacgcc
     6481 attgaaaacg cacagaagca ggtggagtcc cgcaatttcc agacccggaa aaacgtgctc
     6541 cagtacgatg acgtgatgaa cacccagcgg gaggtcatct acaagcagcg ccgccaagtg
     6601 ctggacggcg aggacctcca gcagtccatc cagtccatgc tgcgcagcac cattgagaac
     6661 gccatccgcg gccacatggg agagcagaag catatgactg cggaggagtt ccgggaggct
     6721 accgctctct tccacaccat gttccttttg ccggacgaac tgcggctcac agacgaggag
     6781 ctgcaaggct acactgcgga cgggctggta gatctggtca tggagaaggc ccaggctgtc
     6841 tatcaacgca aggagcagga attcacaccg cccctgatgc gggagctgga gcgggtcatc
     6901 atgctgcggg tggtggacga gtactggatg gaccagatcg acgccatgaa tgacctcaag
     6961 cagggcatcg gactgcgggc ctacgcccag actgaccccg ttgtagccta caaaaaggaa
     7021 ggctacgaga tgttcgagca gatgatcgct gccatccagg aggagaccct gcgccgcctg
     7081 ttcctggtgc gcatccagcg caaggcgcct gatgcccccg ccccggagat caaacgggaa
     7141 cgggtggcca agatcaccag cgagtcaggc gccagcgacg gcacggtcaa aaagcagccg
     7201 gtaaaaaagg cggtcaagat cggccgcaac gacccctgcc cctgcggcag cggcctgaag
     7261 tggaaaaagt gcacctgcaa ggagtatcac agcgagcagc agtaagaacc cacacaaaag
     7321 cagtggaccc gtatctggag caaggcggca taggcgcaca gcttatgccg ccttgctgtg
     7381 taccagcctt tgacgatcag gaggaaaagg agagcacaga tggaacagac agatctgatc
     7441 cgcagggccc gctatggccc caatacaatt ttggcagccg ggatgtccag ggcgctgaac
     7501 acggcgctga attcggcgga aagtgagctg ctcctcctgg tcctgatcct ggaccgggac
     7561 agcggacgca tcctggacgc agagtgcaac atcatcctgg gcatcaacag tgactacatc
     7621 gcatccctcc tggtggggcg gaacttttac caggagctgg acgagatcat cctgtccatc
     7681 cagcagcagt atttcggccc gggtcagcgg gcgctgatcg tctgtctgaa ggatgcgtac
     7741 tccaagatga gggctcgcag cccccgttcc ctgccgctcc gaaagtaggg ccggcgctct
     7801 cttgctctgt ctgattggta aaaaacgaca gtagaacggg aacgggattt tgctttttgc
     7861 acaatttctg ttgacaagag cgatcttttt tattataatt caggcatatg atccgttccg
     7921 cttcccagcg ggccaagggg gctggagggt atatgtccga tttcccttcg ggggccatct
     7981 gtgtcgttgg tctggccaag gtggcgaacc agaacccgat ccacagcgtg caccgcgccc
     8041 tggccggctc ttttatcgtg gacccctcca gcggcctggt gtatgatgtc cagtttgaca
     8101 cagtctgcca gatcaccagc gattttctgg cgtccctgct cattggtctc agcctccaca
     8161 cggacctgga ggagatgatc tcccgggtcc agagccgcta cttcggcgac tcccgccggg
     8221 ctatcaccgt catcctacgg gatgtggctg cccgcctgag ggagtgccaa gggggcgggt
     8281 acaactgaat ctttctttat ttatggtgtt ggaggtctcc cctccaacag ccgaataaat
     8341 agggacctgt ttgattgcct gctggcagcc aagggggtcc ctttgttttt ttagaaatag
     8401 gcctgggaaa cgctccaggc agaggcaggc cccgggaggt gtatgttgaa atgttgccat
     8461 gaaaatgtga gcgatgaccc catccctgtc cccgcatatc ttgtctgcct gtgccggtaa
     8521 aaacaactgt gtgaatcaag aaaggagaag ctgccatgat cttcaacctt ccccctatct
     8581 gcggtctgtt tccgctgctg ctgtacatcg tcctggcatt taaaaaaggg atgcatcctc
     8641 tggtgaacgt gtccatctgc gctgttgtgg gcgcaatcat ggtgaaacag cccctgaacg
     8701 gcctgggcgc ggtgatctac gagtccatgg gctccttcct ggccctggtg ggattcatca
     8761 tcatgctggg ctcaggactg ggcatggtgc tgagaaagac aggcgtagcc gccgccatcg
     8821 tcaatgtgct gatgcggaag atcggcgtcc gtacccagaa gcgggctatg atcgccacca
     8881 tggtctgttc cgtggtgctg gtcaccctgc tgggcacttt ggcaggtgcc aacgccgtga
     8941 tcgcccccat cgtgatcccg ctggtagctg cggtgggcat cactccctcc gcgctggccg
     9001 tcatcttcca gggcgccggt cagaccggac tgttcctcag ccccttctcc ccgcctatgg
     9061 tgaccctgat gagcatcacc gggctctcct atccccgcct tttgctgtgt gcgggactgc
     9121 ccgtatccat cgtcatgtgg gtgggcacct tcctcatcgc caacaaagtc cagaaggaga
     9181 cccaggggac ggagaacttc ggccctgacg gggaggccgc tgcggagaac ttccagatgg
     9241 acccggccac ccgccgcgcc accatcgcct ttgcggtcac tatggctctg atggtgatct
     9301 atgggatcgc caccaatggc ggcgcatcct tcgtgatcct ggtgatgatc gtggctgcga
     9361 tcatcacagg tctgtttgcc agaatgcccg ttggcaccat ctttgacagc atgatggagg
     9421 gctgcggaaa aatgatgtgg ctgttcttca tgttcctcat ttttaacccc ttcctgaact
     9481 ttgtcaccca gtccggtgcc tttgatgccc ttctccagct gctggagcct ctgatcggcc
     9541 ccaccgggaa ggtgggcttc accctgctga ctgtgctggt gggcatcttc ggcatcaacg
     9601 gtgccgccgt ggcccaggcc atgatgatcg acagcctgtt cagctccttc ctcccgaccc
     9661 tgggcatttc catggagctg tggggcatga ttgtcctcat cggtcatcag atcacctcat
     9721 tt
//
LOCUS       DBMDSJ010000014         9646 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2307_length_9646_cov_1.898900, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000014 DBMDSJ010000000
VERSION     DBMDSJ010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9646)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2307_length_9646_cov_1.898900"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="ACZPAQ_00795"
     CDS             complement(<1..175)
                     /locus_tag="ACZPAQ_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0902540.1"
                     /translation="MAKLIQDLSIGGNLRKLREARGLTQEDVCARMAQLGRPMLQSAY
                     SQIETGRRNLFVSD"
     gene            complement(319..1263)
                     /locus_tag="ACZPAQ_00800"
     CDS             complement(319..1263)
                     /locus_tag="ACZPAQ_00800"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR0902541.1"
                     /translation="MADVKKIATRDSYGSALAELGASHPDLIVFDADLAGATKTAVFK
                     KAFPDRHFNCGIAEADMMSAAAGAAAMGLVPFASSFAMFAAGRAFEQVRNSIGYPHLN
                     VKIGATHGGISVGEDGASHQCCEDFALMRTIPGMVVMSPSDDVEARAMVKAAYEHEGP
                     VYMRFGRSAVPVFHEEDGFTFQIGKGEVLMDGSDVAIIATGLLSYEALEAGKALKEAG
                     ISARIINMATIKPLDEELVLKAAQECGKIITCEEHSVIGGLGEAVCALLSEKCPTLVR
                     RIGVNDEFGHSGPAADLLKQFGLSAEHIVEVAKDFCGK"
     gene            complement(1265..2110)
                     /locus_tag="ACZPAQ_00805"
     CDS             complement(1265..2110)
                     /locus_tag="ACZPAQ_00805"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR0902542.1"
                     /translation="MTASEKKQLMANACKVRMGVIESTYGAKSGHPGGSLSASDLFTY
                     LYFREMKVDPADPKWEERDRFVLSKGHTAPGLYAALALKGFFPWEELKTLRHIGSRLQ
                     GHPNMNETPGVDMSTGSLGQGVSAAAGMALAAKYQGKDCRIYTLLGDGEIEEGQPWEA
                     FMFAHQYKLDNLCVIIDNNNLQIDGKITDVMSPYPIPEKLRAFGFQVREIDGHDFEQM
                     ESAFDEARATKGVPFAIIMTTIKGKGVSFMEDVAGWHGKAPNDEEYKTAMDELKARLA
                     EVEGM"
     gene            complement(2404..3066)
                     /locus_tag="ACZPAQ_00810"
     CDS             complement(2404..3066)
                     /locus_tag="ACZPAQ_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902543.1"
                     /translation="MKKLVPVFCAGALLFSLAACVGGETPTGSASGSPDPGTSAQEPA
                     GSQSSAGSSSQQADGTAGGSQAQPQGTEYTLYIGMDGTFQEYPMTYEGELDEQGLVPA
                     EAMIAGMAELTGWNLDLADEITSGPGGMTVSFADTCSIFTGPPDPQKEEFHVYDAGEL
                     AATILDSIQHTLQYNFVDPEKGEPSSLDIYYCDAAGESITIPGLDVTIPMDTPYSGLA
                     MA"
     gene            3413..4552
                     /locus_tag="ACZPAQ_00815"
     CDS             3413..4552
                     /locus_tag="ACZPAQ_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR0902544.1"
                     /translation="MFHYLDNAATTQTRPEAAQAAVTAMTEEWGNPSSRYAFGQEASG
                     RLKEHRAQVAAGLGCRPEEVYFLSCGTEGDNWAIAAAVEKNRRKGKHIITTAIEHAAV
                     LEPIRELERQGYEVTWLQPDQQGIITAEQVEAALRPDTILVAMMLVNNELGTVLPVAE
                     TARAIRAARCPALLHCDAVQGFLKVPFTPEGLGVDTLAVSGHKVHAPKGIGALYIRRG
                     LRLPPLIRGGGQEKGLRSGTEPTAQTAAFAAAVEAGRASLERDLAHMRELKDYAARTL
                     REQVPGLELIGAGTAPHILPVTLPGYKSEVVLRFLSDRGIYVSSGSACHKGKPSHVYA
                     ALKLPKPQLDGILRISFSYDTAREDVDALVQGLKEAQAQLFTSLS"
     gene            4606..5454
                     /locus_tag="ACZPAQ_00820"
     CDS             4606..5454
                     /locus_tag="ACZPAQ_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0902545.1"
                     /translation="MTPELRITVLMENTAREGLEAEHGLSFFLEYRGRRFLLDAGSSG
                     RFADNAQKLGVDLDGVELAALSHGHYDHADGLRAFFALNDRAPVYIRPQAGEAYYSKS
                     GGSPRFVGIHREIWEGFRDRFVPVDGLYPLCPGAWLAPETVRGGPFASREPNLLRKVG
                     EDRFLPDDFSHEQSMVLETGRGLVVLNSCCHGGVVNIVKGVLDQFPGQRVYALLGGLH
                     MLSPGANALNCPPDYVRQVAAALLELGVEHLWTGHCTGPAAFALLKEELGDRVEPLTG
                     GMRLGI"
     gene            5661..6734
                     /gene="hrcA"
                     /locus_tag="ACZPAQ_00825"
     CDS             5661..6734
                     /gene="hrcA"
                     /locus_tag="ACZPAQ_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR0902546.1"
                     /translation="MELTDRKKRVLRAIVETYINTAEPVGSKAIAQLAGLDVSAATIR
                     NEMADLTEMGLLEQPHTSAGRVPSAAGYRLYGNELMGQHQLTLQETERINRALNLKME
                     ELDRVIDQAGKVLSQISDYPVFSIAAGPERVTVKRYDLLMVEKGAFIVVVMTDTSVVK
                     NKIIRVPGELSETQLQLLSAVLNSSFVGLELAEMLEALEKMEAHSAPGAFEMISLVVD
                     FAMEILEEQSRKTVHTAGITHLLEHPEYHSLDKAKPLMTYLSEESEASKLPVALDGGR
                     NMNILIGPENVNEALKDTSVVMASYDIGDNMRGVIGVVGPTRMDYAKVTARLSYFADS
                     LTRMFGKGELPSGEGDGDGQAQE"
     gene            6751..7311
                     /gene="grpE"
                     /locus_tag="ACZPAQ_00830"
     CDS             6751..7311
                     /gene="grpE"
                     /locus_tag="ACZPAQ_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR0902547.1"
                     /translation="MSQKEKNKTPPELDDQLEEAAAKEAAEEPAEAEAGATDPLMDEL
                     EALKDQAARQEEKYLRLAAEYDNFRKRTAKEKEAIWNDAKADAALAFLPVYDNLERAL
                     KQETADEAYKKGVEMTMNQLKTVLEKLGITEIEALGQPFDPTLHNAVMHIEDENFGEN
                     TVAEVFQAGFRLGDKVVRFAMVKVAN"
     gene            7340..7477
                     /locus_tag="ACZPAQ_00835"
     CDS             7340..7477
                     /locus_tag="ACZPAQ_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902548.1"
                     /translation="MRDPMIRKKACRQCLCLDPAVYALFLTASMCFLFSFVFYLLSFI
                     F"
     gene            7519..9375
                     /gene="dnaK"
                     /locus_tag="ACZPAQ_00840"
     CDS             7519..9375
                     /gene="dnaK"
                     /locus_tag="ACZPAQ_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR0902549.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGGEAVVIPNAEGNRTTPSVVAFSK
                     DGERMVGQVAKRQAITNPDRTIISIKREMGSDYKVSIDGKKYTPQEISAMILQKLKAD
                     AEAYLGETVSEAVITVPAYFTDAQRQATKDAGKIAGLDVKRIINEPTAAALAYGVDKE
                     ESQKVMVYDLGGGTFDVSILDIDDGVIEVLATAGNNRLGGDDFDKCVMDWMAAEFKKT
                     EGIDLTGDKVAMQRLKEAAEKAKIELSGVTSTSINLPYITADATGPKHLDLTLSRAKF
                     DQLTAHLVEATAGPVRQAMNDAGLSGSDIHKVLMVGGSSRIPAVQEMVKKLTGKEGFK
                     GINPDECVAMGAALQGGVFTGDVKDLLLLDVTPLSLGIETMGGVMTKLIERNTTIPVK
                     KSQIFTTAADNQTSVEVHVLQGEREMAQYNKTLGRFHLDGIAPARRGVPQIEVTFDID
                     ANGIVNVSAKDLGTGKEQHITITSSTNMSKDDIDKAVREAEQFAAEDKKQREAADIRN
                     QADQMVFQTEKTLEDMGDKIPASDRNSVESALNHLKETQKGSDVEAIKAATEELTKAF
                     YAVSEKLYQQNGGQAGPGPDMGGANFGGQAGGSGAGPDVVDADYEVVDDDKK"
     gene            9552..>9646
                     /locus_tag="ACZPAQ_00845"
     CDS             9552..>9646
                     /locus_tag="ACZPAQ_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR0902550.1"
                     /translation="MPEQKRDYYEVLGVSKGASDEEIKKAYRKLA"
BASE COUNT         1914 a         2926 c         3103 g         1703 t
ORIGIN      
        1 agtcgctgac gaacagattt ctccgcccgg tctcgatctg ggaataggcg gactggagca
       61 tgggtctgcc aagctgtgcc atcctggcgc acacatcctc ctgggtcagc ccccgggcct
      121 cccgcagttt ccgcaggttc ccgccgatgc tgaggtcctg tatcagcttt gccataatcg
      181 ctcctttcaa attattgcta ttgcgatttt ctcgatcaca gcaaatgcta tggcgcaatg
      241 atcactatct ggcgataatt atatttttgc ccaataaaag ccccctgtcc cgagggggaa
      301 cagggggccg ccgtccgctt atttgccgca gaagtccttg gcgacctcca cgatatgctc
      361 cgcgctcagg ccgaactgct tgagcaggtc ggcggcggga ccggagtgtc cgaactcgtc
      421 gttgacgccg atgcggcgca ccagggtggg gcacttctcg ctgagcaggg cgcacaccgc
      481 ctcgcccagg ccgccgatga cggagtgctc ctcgcaggtg atgatctttc cgcactcctg
      541 ggccgccttc agcaccagct cctcgtccag aggcttgatg gtggccatgt tgatgatccg
      601 ggcgctgatc ccggcttcct tcagggcctt gcccgcctcc agcgcctcat aggagagcag
      661 gccggtggcg atgatagcca cgtcggagcc gtccatgagc acctcgccct tgccgatctg
      721 gaaggtgaag ccatcctcct cgtggaacac cggcacggcg gagcggccga agcgcatgta
      781 cacggggccc tcatgctcat aggccgcctt caccatggcc cgggcctcca cgtcgtcgga
      841 gggggacatg accaccatgc cggggatggt gcgcatcagg gcgaagtcct cacagcactg
      901 gtgggaggcc ccgtcctcgc ccacggagat gccgccgtgg gtggcaccga tcttcacatt
      961 caggtggggg tagccgatgg agttgcgaac ctgctcaaag gcgcggccgg cggcaaacat
     1021 ggcgaagctg gaggcaaagg ggaccagtcc catggcggcg gcgccggcgg cagcagacat
     1081 catatccgcc tcggcgatgc cgcagttaaa gtggcggtcg gggaacgcct tcttgaatac
     1141 ggcggtcttg gtggcgccgg ccaggtcggc gtcgaacacg atcaggtcgg gatgggaggc
     1201 gcccagctca gccagcgcgc tgccatagct gtcccgggtc gcgatcttct tcacatctgc
     1261 catcttacat cccctccact tctgccaggc gggctttcag ctcgtccatg gcggtcttgt
     1321 actcctcgtc gttgggggcc ttaccgtgcc agcctgccac gtcctccatg aaggagaccc
     1381 ccttgccctt gatggtggtc atgatgatgg cgaaggggac gcccttggtg gctctggcct
     1441 cgtcaaaggc ggactccatc tgctcgaagt cgtggccgtc gatctcccgg acctggaagc
     1501 cgaaagcccg cagcttctcc gggatggggt agggactcat cacgtcggtg atcttcccgt
     1561 cgatctgcag gttgttattg tcgatgatga cgcacaggtt gtccagctta tactggtggg
     1621 caaacatgaa ggcctcccag ggctggccct cctcgatctc gccgtcaccc agcagggtgt
     1681 agatccggca gtccttcccc tggtacttgg cggccagggc catgccggcg gcggcggaca
     1741 cgccctggcc cagggagccg gtggacatgt ccacgccggg ggtctcgttc atgttggggt
     1801 gtccctggag gcggctgccg atgtggcgca gggtcttcag ctcctcccag gggaagaagc
     1861 ccttcagggc cagggcggcg tacaggccgg gggcggtgtg tcccttggaa aggacgaagc
     1921 ggtcccgctc ctcccacttg gggtcggcgg ggtccacctt catctcccgg aagtacagat
     1981 aggtaaacag gtccgaggcg gacaggcttc cgccgggatg gccggatttg gcgccatagg
     2041 tggattcgat gacgcccatg cgcaccttgc aggcgttggc catcagttgc tttttctcac
     2101 tggctgtcat gacgttctcc ttcttcttcg atttccggtc gcaggtcctt ctctctcttc
     2161 ccgcggccgg agcgtttcta tgcgtattat aaaacagtcc ttccaaaagt gcaagggacc
     2221 gtccgcttac tttccatgaa acgatgggat ggacccatgc ataaaaaaga aaagaggctc
     2281 cctgttccca aagggggaga cagggagcct cagtttgccg aaaaaagcct cgcagagttc
     2341 gcgcctgcgc gcggcagact gcatctttct cgaaaaagcg gctttgccgc tttttcgacc
     2401 gtctcatgcc atcgccagcc cggagtaagg ggtgtccatg gggatggtca cgtccaggcc
     2461 tgggatggtg atgctctccc cggcggcgtc acagtagtag atgtccaggg aagagggctc
     2521 ccccttctcc gggtccacaa agttgtactg gagggtatgc tggatgctgt ccaggatggt
     2581 ggccgccagc tcccccgcgt catacacgtg gaactcctcc ttctgggggt ccggcggacc
     2641 ggtaaagatg gagcaggtgt cggcaaaaga caccgtcatg ccgcccggac cgctggtgat
     2701 ctcgtccgcc agatccaggt tccagccggt gagctccgcc ataccggcga tcatggcctc
     2761 cgcgggcacc aggccctgct cgtccagctc cccttcatag gtcatagggt attcctgaaa
     2821 tgtcccatcc atgccgatgt acagggtgta ctctgtcccc tggggctggg cctggctccc
     2881 gccggccgtg ccgtccgcct gttgactgga actgccggcg gaggactggg agcctgccgg
     2941 ctcctgggcg gaggtgccgg ggtccgggct cccggacgcg ctccctgtcg gcgtctcgcc
     3001 tccgacgcag gcggccagac taaacagcag ggctccggca caaaatacag gtactagctt
     3061 tttcatcgtt tcctcctttt ccacgatggc cttgggtctc tcactccgct ctggagtgat
     3121 tggggctatc atagccacat ccgcctccgc cgtcaaggaa ttttccccaa ccttcacact
     3181 ttggtcacgg aatctttacc gcccggtcaa aaaagcccgc tctctgaccg gaggaatatt
     3241 ttatcaaatg gacgaatttg ggaaaagggg caaggcagtg ccagtccttc tcctctggag
     3301 gggaaggact ggggcgcttt tctgtcaggc cggagcaggc gggatttctt gactttccgc
     3361 agaagtgggc atattatgaa aaatacttga tcgtcagggg gaaatcagaa ccatgtttca
     3421 ctatctggac aatgccgcca ccacacagac ccggccggag gccgctcagg cggctgtgac
     3481 cgccatgacg gaggagtggg gcaacccatc ctcccggtac gccttcggcc aggaggcgtc
     3541 gggacggctg aaggagcatc gggcacaagt ggccgccggc ctgggctgcc gcccggagga
     3601 agtgtacttc ctctcctgcg gcactgaggg ggacaactgg gccattgccg ccgcagtgga
     3661 gaagaaccgg cggaaaggaa aacacatcat caccaccgcc atcgaacacg ccgcggtgct
     3721 ggagcccatc cgggagctgg aacggcaggg ctatgaggtc acctggctcc agccggacca
     3781 gcaagggatc atcacggcag agcaggtgga ggcggccctg cgcccggaca ccatcctggt
     3841 ggccatgatg ctggtgaaca acgagctggg caccgtcctc ccggtggccg agaccgcccg
     3901 cgccatccgt gcggcccgct gccccgccct gctccactgt gacgcggtcc agggctttct
     3961 gaaggtcccc ttcaccccgg aggggctggg ggtggacacc ctggcggtga gcgggcacaa
     4021 ggtccacgct cccaagggca tcggggccct gtatatccgc cgggggctga ggctgccgcc
     4081 gctgatccgg gggggcggac aggagaaggg actgcgttcc ggcacggagc ccacggccca
     4141 gaccgccgcc tttgccgccg cggtggaggc ggggcgggcc agcctggagc gggacctggc
     4201 ccacatgcgg gaactgaagg actacgccgc ccgcaccctg cgggagcagg tgccggggct
     4261 ggagctgatc ggggcgggga ccgccccaca catccttcct gtcaccctgc ccggctacaa
     4321 gagcgaggtg gtgctccgct tcctcagcga cagggggatc tatgtctcct ccggctccgc
     4381 ctgccacaag ggcaagccca gccatgtgta cgcggccctg aagctgccca aaccccagct
     4441 ggacggcata ctgcggatca gcttctccta cgacaccgcc cgggaggacg tggacgccct
     4501 ggtccagggg ctgaaggagg ctcaggccca gctgttcacc agtttgtcct gaggggagcg
     4561 tcagacacag aagaaaacaa acaaagaata aaggagtgga gcgggatgac accggaactg
     4621 cggatcacgg tcctgatgga aaatacggcc cgggaggggc tggaggcgga gcacggcctg
     4681 tccttcttcc tggagtaccg gggcaggcgc tttctgctgg atgcgggctc ctccggccgg
     4741 tttgcggaca atgcccaaaa gctgggggtg gacttggatg gggtagaact ggcggctctg
     4801 tcccacggac actacgacca tgccgacggc ctgcgggcct tttttgccct caatgacagg
     4861 gccccggtgt atatacggcc ccaggcgggg gaggcttact actccaagtc gggaggctcc
     4921 cccagatttg tgggcatcca tcgggagatc tgggagggct tccgggaccg cttcgtgccg
     4981 gtggatgggc tgtaccccct ctgccccggg gcctggctgg cgccggagac cgtccggggc
     5041 gggccctttg ccagccggga gcccaatctg ctgcgcaagg tgggggagga ccggttcctg
     5101 cccgacgact tttcccacga gcagtccatg gtgctggaga ccgggcgggg gctggtggtg
     5161 ctcaactcct gctgccatgg gggtgtggta aacatcgtga agggcgtgct ggaccagttc
     5221 ccgggacagc gggtgtatgc cctgctgggc ggcctgcata tgctcagtcc tggggccaat
     5281 gcgctcaact gtcccccgga ctatgtccgc caggtggcag ccgcgctgct ggagctgggt
     5341 gtggagcacc tgtggacggg gcactgcact ggtcctgccg cctttgcact gctgaaggag
     5401 gagctggggg accgggtgga gcccctcacc ggcggaatgc ggctgggtat ttaaggagcg
     5461 ggatatcgtc catgtttgga cggactcatt agcaatttca gaagccctct gcaaaaaaca
     5521 gatcgcagag ggcttcttcc gtttgttcaa aataaattca taaagtaact attgacaatt
     5581 tggaggtcag gtggtactct atcgttagca ctcagggaat atgagtgcta aaattagaaa
     5641 aacaccgagg tgagcgtgca atggaactga ccgaccggaa aaagcgggtc ctgcgggcca
     5701 tcgtggagac ctatatcaac accgccgagc cggtgggctc caaggccatc gcccagctgg
     5761 ctggattgga tgtgtccgcc gccaccattc gcaatgagat ggcagacctg accgagatgg
     5821 ggctgctgga acagcctcac acctccgccg ggcgggtccc ctccgccgca ggttaccgtt
     5881 tgtatggcaa cgagttgatg gggcagcacc agctgaccct tcaggagacg gagcggatca
     5941 acagagcgct gaacctgaag atggaggagc tggaccgggt catcgaccag gccggcaagg
     6001 tcctctccca gatcagcgac tacccggtgt tctccatcgc ggccggccca gagcgggtga
     6061 cagtgaagcg gtacgatctg ctgatggtgg agaagggcgc tttcatcgtg gtggtgatga
     6121 ccgacaccag cgtggtgaaa aacaagatca tccgggtgcc gggagagctg tccgagactc
     6181 agctccagct gctctccgcc gtgctcaaca gctcctttgt ggggctggaa ctggctgaga
     6241 tgctggaggc actggaaaag atggaggccc actccgcccc aggtgccttt gagatgatct
     6301 ccctggtggt ggactttgcc atggagatcc tggaggagca gagccgcaag acagtacaca
     6361 ccgccgggat cacccacctg ctggagcacc cggagtatca cagcctggac aaggctaagc
     6421 cgctgatgac ctatctttca gaggagtcgg aggcatccaa gctgccggtc gccctggacg
     6481 gcggcaggaa catgaacatc cttatcggcc cggaaaatgt gaacgaggcc ctgaaggaca
     6541 ccagcgtggt gatggccagc tatgacatcg gcgacaacat gcggggcgtc attggtgtgg
     6601 tgggccccac ccgtatggat tatgcaaagg tgaccgcccg cctgtcctac ttcgccgaca
     6661 gcctgacccg gatgttcggc aagggagagc tcccctccgg agaaggagac ggggacgggc
     6721 aggcgcagga ataggaggaa aggatagccc atgagtcaga aagaaaagaa caagacgccc
     6781 cccgagctgg acgatcagct ggaggaggcg gccgcgaagg aagcggctga ggagccggcc
     6841 gaagcggagg ccggggccac cgaccctctg atggatgagc tggaggcgct gaaggaccag
     6901 gcggcccggc aggaggagaa atatctccgg ctggcggcgg agtacgacaa cttccgcaag
     6961 cggactgcca aggagaagga ggccatctgg aacgacgcca aggcggacgc cgcgctggca
     7021 ttcctcccgg tgtacgacaa tctggaacgg gccctgaagc aggagactgc agacgaggcg
     7081 tataaaaagg gcgtggagat gaccatgaac cagctgaaga ctgtgctgga aaagctgggc
     7141 atcaccgaga tcgaggccct gggacagccc tttgacccca cccttcacaa cgcggtaatg
     7201 cacattgaag atgagaattt tggagaaaat accgtggccg aggtgttcca ggccggcttc
     7261 aggctgggag acaaggtggt ccgctttgcc atggtgaagg tcgccaactg acggaaaaca
     7321 gaatggagga gacagctcag tgagagatcc catgatccgg aaaaaagcct gccggcagtg
     7381 cctctgcctg gacccggccg tctacgccct gtttctgacg gccagcatgt gcttcctgtt
     7441 ctcttttgtc ttttatctgc tctctttcat cttttgacct tgtaaataca aatcttttca
     7501 tattggagga acgaaattat gtccaagatc attggtatcg acttaggtac gacaaacagc
     7561 tgtgtggctg tcatggaggg cggcgaggcc gtcgtcatcc ccaacgcgga gggcaaccgc
     7621 accaccccct ctgtagtggc attttccaag gacggcgagc gtatggtggg ccaggtggcc
     7681 aagcgccagg ccatcaccaa ccccgaccgc accatcatct ccatcaagcg tgagatgggc
     7741 tctgactaca aggtgagcat cgacggcaag aagtacaccc ctcaggagat cagcgccatg
     7801 atccttcaga agctgaaggc ggacgccgag gcctatctgg gtgagaccgt ctccgaggcg
     7861 gtcatcaccg tccccgccta ctttaccgac gcccagcgcc aggccaccaa ggatgcgggc
     7921 aagatcgccg gcctggatgt gaagcggatc atcaacgagc ccaccgccgc ggccctggcc
     7981 tacggcgtgg acaaggagga gtcccagaag gtcatggtct acgacctggg cggcggcacc
     8041 ttcgacgtat ccatcctgga catcgatgac ggcgtcatcg aggtgctggc caccgccggc
     8101 aacaaccgct tgggcggcga tgacttcgac aagtgtgtca tggactggat ggccgccgag
     8161 ttcaagaaga ccgagggcat cgacctcacc ggcgacaaag tggcgatgca gcgcctgaag
     8221 gaggccgccg agaaggctaa gatcgagctt tccggcgtca cctccaccag catcaacctg
     8281 ccctatatca ccgccgacgc caccggcccc aagcatctgg acctgaccct gagccgggcc
     8341 aagttcgacc agctcaccgc ccacctggtg gaggccaccg ccggccctgt ccgtcaggcc
     8401 atgaacgacg ccggcctgtc tggcagtgac atccacaagg tgctgatggt gggcggctcc
     8461 agccgtatcc ccgccgtgca ggagatggtg aagaagctca ccggcaagga gggcttcaag
     8521 ggcatcaatc ccgacgagtg cgtggccatg ggcgcggccc ttcagggcgg tgtgttcacc
     8581 ggcgacgtaa aggacctgct gctgctggac gtcacccccc tgtcccttgg cattgagacc
     8641 atgggcggcg tgatgaccaa gctgatcgag cgcaacacca ccatcccggt caagaagagc
     8701 cagatcttca ccaccgccgc cgacaaccag acctccgtgg aggtccacgt gctccagggc
     8761 gagcgtgaga tggcccagta caacaagacc ctgggccgct tccacctgga cggcatcgcc
     8821 cccgcccgcc ggggcgtgcc gcagatcgag gtcaccttcg acatcgacgc caacggcatc
     8881 gtgaacgtgt ccgccaagga tctgggaacc ggcaaggagc agcacatcac catcacatcc
     8941 tccaccaaca tgtccaagga cgacatcgac aaggccgtgc gggaggcgga gcagttcgcc
     9001 gccgaggaca agaaacagcg ggaggcggcc gacatccgca accaggcgga ccagatggtg
     9061 ttccagacgg aaaagaccct ggaggacatg ggcgacaaga tccccgcgtc cgacaggaac
     9121 agcgtggaga gcgcactgaa ccacctgaag gagacccaga agggcagcga cgtggaggcc
     9181 atcaaggcag ctaccgagga gctgacgaag gcgttctacg ccgtcagcga gaagctgtat
     9241 cagcagaacg gcggtcaggc cggccccggc ccggacatgg gcggcgccaa tttcggcggc
     9301 caggcgggcg gcagcggcgc cggacccgac gtggtggacg ccgactatga ggtcgtggac
     9361 gacgacaaga aataaaaacg ttcatggtcc gcgcgggcgg ggcatcgccc cgcctgcgcg
     9421 cccttgcgtt tccataagag ggaagagcga agtgtggcga gtgaagagat gagcaccccg
     9481 tttcactggg gaggcaggag ctcatctcca ccttcccagc tccgtttttc actctgaata
     9541 gaggtgatac tatgccagaa cagaagcgag actactatga ggtcctgggc gtgagcaaag
     9601 gtgcctctga cgaagagatc aaaaaggcct accggaagct ggccaa
//
LOCUS       DBMDSJ010000015         9581 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2326_length_9581_cov_1.684177, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000015 DBMDSJ010000000
VERSION     DBMDSJ010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2326_length_9581_cov_1.684177"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="ACZPAQ_00850"
     CDS             <1..1137
                     /locus_tag="ACZPAQ_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR0902551.1"
                     /translation="MIFSACEQTKKPPFHTVFIHGLVRDDKGRKMSKSLGNGIDPLEI
                     ADKYGADALRFNLVTGNSPGNDMRFYTERCEAMRNFANKIWNASRFLMMNLTIDACQL
                     PGKLELEDKWILSKLNTAIREITENMDRYELGVAAQKIYDFIWDDYCDWYIELTKTRL
                     QGEDEDSKVRAQQVLCHVLTQMLKLLHPFMPFITEEIWQALPHEGDFLMLSDWPVADA
                     ALDFPEEEKAMELIMDAIRGVRTRRSEMNVPPSKKAHLTVATGERDIFVLGVPFLKKL
                     AYASEVTFAQPGTSPEAGSVTVVTHAAQISMPLAELVDLEKEKARMEKELKKNSDELQ
                     KLNTKLANPGFVNKAPEHVVKAEQERAVKLTELVAKLEEQLKTM"
     gene            1498..1806
                     /locus_tag="ACZPAQ_00855"
     CDS             1498..1806
                     /locus_tag="ACZPAQ_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HIR0902552.1"
                     /translation="MAVELVPEWMAGLEEEDLVFIKKFVLSSGSLKEVAGQYGVTYPT
                     VRLRLDRLIQKIKLGEDRAADPYIALIKRLAVNERLDFDTAKLLIAAYKKETRKDEMT
                     "
     gene            1821..2231
                     /locus_tag="ACZPAQ_00860"
     CDS             1821..2231
                     /locus_tag="ACZPAQ_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902553.1"
                     /translation="MTILFVLPVVLLSMLLPLALVVALQVWLCRKGKWLGLILPAITL
                     AMSLMICLSVVGFSAVTGGGSVQVTDGHGNIVEEYHREEEHHQLQPADLAMVGGVFLV
                     FNVPTLVLGGIWLYYKNRREWKEDLKKMNIQDLE"
     gene            2359..2730
                     /locus_tag="ACZPAQ_00865"
     CDS             2359..2730
                     /locus_tag="ACZPAQ_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR0902554.1"
                     /translation="MPLHESGEDYLEAILVLHEQKGNVRSIDVAQHLGYSKPSVSRAM
                     SILRSSGYITMERDGRILLTEAGKTAAESVYERHRFLTKWLIQLGVTPEVAAEDACRI
                     EHDISPETFACLKRHAGGGES"
     gene            2860..3681
                     /locus_tag="ACZPAQ_00870"
     CDS             2860..3681
                     /locus_tag="ACZPAQ_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR0902555.1"
                     /translation="MSTEELVSGVLEGLKLNALDLSALFRTALLILAGVVVIRVLLRI
                     VDRMLSRSQNLGSVGGYIRSVVRIALWFLLVLVVADSLGIPVASMIALLSVAGLAISL
                     ALQNTLSNLAGGIMILVSKPFEVGDYVEADGVGGTVISIGLAYSTLATVDNKEIFIPN
                     SQVAAAKIINYTKLGKRRVELTFSASYDAPTETVKQALGEVLAQFPQILEDPAPSIWI
                     SKYGASSIEYVVRVWTAAGDYWDVYYGVMEGVRPAFQRHGIQMTYDHLNVHLLEK"
     gene            complement(3719..4198)
                     /locus_tag="ACZPAQ_00875"
     CDS             complement(3719..4198)
                     /locus_tag="ACZPAQ_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HIR0902556.1"
                     /translation="MIILTAKLPRHRKLTIGVAVAALACCAALVLTLGGGPAREASAS
                     AVPSPKGVKSNEDRVAYLQSYGWEVSAEPLATEELLIPKEMDASYDDYLALQSGQGFD
                     LSKYAGKRVKRYTYEITNYPTGETGILANLLIYKNTVIGGEVLSPQLDGFLHGLAMP"
     gene            4340..5083
                     /locus_tag="ACZPAQ_00880"
     CDS             4340..5083
                     /locus_tag="ACZPAQ_00880"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR0902557.1"
                     /translation="MAQERLDKLLSSTGRWSRREVKELIRQGRVWVDGVPARRPEEKC
                     EPGAAGLTVDGERVDCAPFVYLMLHKPAGLLSATEDRRQKTVLDLLPEHLRRRGLFPV
                     GRLDKDTEGLLLLTDDGPLGHALLSPKNHVDKVYYAKVEGRLDRTDAEALAAGMVLGD
                     GLHCLPAELEPLGDGSQCHVTLREGKYHQVKRMLAARGKPVVYLKRLSMGPIALDPAL
                     APGEWRPLTGEEKALLRRGTAGDPGFSAF"
     gene            5173..6264
                     /locus_tag="ACZPAQ_00885"
     CDS             5173..6264
                     /locus_tag="ACZPAQ_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR0902558.1"
                     /translation="MSSRMFQGVVLQMKDGIDRVVGVIDADGIVVACSELTSIGEHWS
                     GAVAAINAADNGITRYEGKTFKPLAGWGAQFDYAAFVQGEDPESAALCAMAVVAFNGA
                     KTSYEEKHDKATFVKNIISDNILLGDIYTQAKELHFVSEVPRAAFLVRQLGQADVSTI
                     DVIQNLFPDKQTDFVISINETDVALIKQLPEGADAKELQKIAKQISTAVNQELGIKVV
                     IGIGTVVNHIRDLARAYKEAGVAIDVGKVFDTEKTVINYENLGIGRLIYQLPTILCQM
                     FLQEVFKKNPIDALDQETLLTINKFFENNLNVSETARKLFVHRNTLVYRLEKIKKLTG
                     LDLREFDDAITFKVALMVKKYLISRGIES"
     gene            6560..7252
                     /gene="ftsE"
                     /locus_tag="ACZPAQ_00890"
     CDS             6560..7252
                     /gene="ftsE"
                     /locus_tag="ACZPAQ_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR0902559.1"
                     /translation="MIRFIDVYKEYDNGTKALKGVSMRIDDGEFVFLVGPSGSGKSTI
                     IKLITGEIATTEGRLMVNGYNMNNIRPGQVPYMRRTLGIIFQDFRLIEKKTVYENLSF
                     AMRAIGTSTRELRRRIPYVLELVGLEKKADQYPGELSGGEQQRVAIARALVNNPSMII
                     ADEPTGNLDPQRSLEIMMLLERINELGTTLLVVTHEKSLVNRFSKRVVAIENGRIISD
                     ETGGYYNNGETI"
     gene            7236..8126
                     /gene="ftsX"
                     /locus_tag="ACZPAQ_00895"
     CDS             7236..8126
                     /gene="ftsX"
                     /locus_tag="ACZPAQ_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR0902560.1"
                     /translation="MARRFNGKYYLSEGFHSIFTHGFMSFAAVCMIVACLLIMGSFTL
                     LAVNLDHMLGDLEDENLFLAYIDDSYSEEQARALQPKIEAVPNVSQVTFVTRAEALED
                     FKAGREQNDLMDSLPPEVLRDRFEIHVKDIEQMAATVSQVEAVRGVAHTQAAIEIAQG
                     FVMVRNIATGVAAVLVAMLVVVSLFIIANTIKLATFYRREEIAIMKMCGATNGFIRWP
                     FVVEGMILGLTGALVAFFIQWGLYEAVSRLILQTNGLSLITVLSYASMAGNIVLVFCG
                     VGLVIGVGGSVVAIRKFLQV"
     gene            8256..9503
                     /locus_tag="ACZPAQ_00900"
     CDS             8256..9503
                     /locus_tag="ACZPAQ_00900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR0902561.1"
                     /translation="MKRRAIPAISLALVVLFSASFLLALPGAVSMPASAVTQAEIDAL
                     KESAGELEQQQKEIQAQLNAIAADKDEALEQKSLLEQQINVIQSEINNIDAQIAKYDE
                     LISQKEDELAQLEQQEAEQYQLFCERVRYMEEEGEVSYWAILFNAEDFSDMLDRFMMV
                     EEIMAYDNAIMDELLATQAQIEQEKAELEEARTAQEEARQKQADAKARLKSQQAEVDA
                     LISQISSQEDELEAMEAKLQAAAKAMDNEIARKERELQAQLAAQGVTIVSEKGFKWPS
                     YATTITSLFGSRTHPVTGRPNNHTGVDIAAAGGTDIWAAKSGVVLTSTYNSSYGNYVV
                     ISHANGESTLYAHMSKRLVSEGDKVTQGQTIGLVGTTGSSTGNHLHFEVRLNGQRQDP
                     LNYFRGSTFTLRASGKSVSYTVS"
BASE COUNT         2005 a         2713 c         3131 g         1732 t
ORIGIN      
        1 atgatcttct ccgcctgcga gcagaccaag aagcccccct tccacactgt gttcatccac
       61 ggcctggtgc gggacgacaa gggaaggaag atgtccaaga gcctgggcaa cggcattgac
      121 cccctggaga tcgccgacaa gtacggcgcc gacgccctgc gcttcaacct ggtcaccggc
      181 aacagccccg gcaacgacat gcgcttctac accgagcggt gtgaggctat gcgcaacttc
      241 gccaacaaga tctggaacgc cagccgcttc ctgatgatga acctgaccat cgacgcatgc
      301 cagctgcccg ggaagctgga gctggaggac aagtggatct tgtccaagct gaacactgcc
      361 atccgtgaga tcaccgagaa catggaccgg tatgagctgg gcgtggcggc ccagaagatc
      421 tatgacttca tctgggacga ctactgcgac tggtatatcg agctgaccaa gacccgtctc
      481 cagggggagg acgaggacag caaggtgagg gcccagcagg tgctgtgcca tgtgctcacc
      541 cagatgctca agctgctcca tcccttcatg cccttcatca ccgaggagat ctggcaggcc
      601 ctgccccacg agggggactt cctcatgctg tccgactggc cggtggcaga tgccgctctg
      661 gacttcccgg aggaggagaa ggccatggag ctgatcatgg atgccatccg tggggtgcgc
      721 acccgccggt ctgagatgaa cgtgcccccc agcaagaagg cccacctgac ggtggccacc
      781 ggggagcggg acatctttgt cctgggcgtg cccttcctga agaagctggc ctatgccagc
      841 gaggtgacct tcgcccagcc gggcacctcc ccggaggcgg gctccgtcac cgtggtcacc
      901 catgcggccc agatctccat gcctctggcg gagctggtgg acctggagaa ggagaaggcc
      961 cgcatggaga aggagctgaa aaagaacagc gacgagctcc agaagctgaa caccaagctg
     1021 gccaatccgg gctttgtcaa taaggccccc gagcatgtgg tcaaggcgga gcaggagcgg
     1081 gccgtgaagc tcaccgagct ggtggccaag ctggaggagc agctcaagac catgtgatcc
     1141 ggcacaggag ctgtgaacgc cggaggataa gagaaaacga tctgacgggc cggaccgcgg
     1201 gagaccgcgg tccggctttc cgcgtctttt tgaggtggaa aaaaggaaaa gctgcttcgg
     1261 gggatctttc cggcggccgc ttccccgttc ctagccccat aaggccggat gcccggagcc
     1321 ggtcatgccc gcccggtatg gcgggcatac agtgtacccc cctgtgcggc tggctggggc
     1381 ttttcggtat ccctttcatc agaaagaagt aaaaaagaat tttattaaaa taaaaattta
     1441 ggattgactt taataaaatt aaagtatata ataaatattg tgaaaggggg taaaaggatg
     1501 gcagtggaac tggtgcccga gtggatggcc ggtctggagg aggaggacct ggtgttcatc
     1561 aaaaaatttg tgctgtcctc cggctccctc aaagaggtgg cgggacagta cggcgtcacc
     1621 tatcccacgg tgagactgcg gctggaccgg ctgatccaga agatcaagct gggggaggac
     1681 agggcggccg acccctacat tgccctgatc aagcgtctgg cggtaaatga gcggctggac
     1741 tttgacacag ccaagctgct gattgccgcc tataagaagg aaacaagaaa ggatgagatg
     1801 acgtgatgag cggttccata atgacaatat tgtttgtgct ccctgttgtt ctgctctcta
     1861 tgctgctgcc cctggccctg gtggtggccc ttcaggtctg gctgtgcagg aagggaaagt
     1921 ggctggggct gatcctgccc gccatcaccc tggcgatgag cctgatgatc tgcctgtcgg
     1981 tggtcggctt ctccgctgtc accggcggcg ggtccgtgca ggtcacggac gggcacggga
     2041 atatcgtgga ggagtaccac cgggaggagg agcatcacca gctccagcct gcggatctgg
     2101 ccatggtggg cggtgtgttc ctggtattca acgtccccac tctggtgctg ggcgggatct
     2161 ggctctacta caagaaccgc cgggagtgga aggaagacct gaagaaaatg aatatccagg
     2221 atctggagta aaaggtgggg cgtcccgggg agggacgccc cttttgcggg gatcgcggtt
     2281 gaaatccgtt ccaaaacatg ataatataac tcaataaaaa gcagatccgc cgcaggcgga
     2341 catgagagga gagtccctat gccgctgcat gaatcaggag aagattatct ggaggccatc
     2401 ctggtgctcc atgagcagaa gggcaacgtc cggtccatcg acgtggccca gcacctgggc
     2461 tactccaagc catctgtgag tcgggctatg tctatcctgc gctccagcgg gtatatcacc
     2521 atggaacggg acggccgcat cctgcttacc gaggcgggaa agacggcggc ggagtcggtg
     2581 tacgagcgcc accgcttcct gaccaagtgg ctgatccagc tgggcgtgac gccggaggtg
     2641 gcggcagagg acgcctgccg catcgagcac gacatcagcc cggagacctt cgcctgcctg
     2701 aaacggcacg cggggggcgg ggagagctga cgctccgaac agaaaaagtg acgcggcgga
     2761 ccgagtcccg gcctgccgcg tttctccttg cctgagggga aaaagcttgt tataataaaa
     2821 accaaaatac agatcctgtc gaactggggg aaaactgcca tgtctaccga agaactggtc
     2881 tccggcgtgc tggaagggct gaagctgaat gccctggacc tgagcgccct gttccgcact
     2941 gccctgctca ttctggcggg ggtcgtggtg atccgggtcc ttctgcggat cgtggaccgg
     3001 atgctgtcca gatctcagaa cctggggtca gtggggggct atatccgctc tgtggtgcgg
     3061 atcgccctgt ggttcctcct ggtcctggtg gtggcggact cccttggcat cccggtggcc
     3121 tccatgatcg ccctgctcag cgtggccggc ctggctatct ccctggcgct ccagaacacc
     3181 ctatccaacc tggcgggggg catcatgatc ctggtgtcca agcccttcga ggtgggggac
     3241 tatgtggagg cggacggggt ggggggcacg gtcatttcca tcggcctggc gtacagcacc
     3301 ctggccaccg tggacaacaa ggagatcttt atccccaaca gccaggtggc ggcggccaag
     3361 atcatcaact ataccaagct gggcaagcgg cgggtggagc tgaccttctc cgcctcctac
     3421 gatgcgccga ctgagacggt gaagcaggcg ctgggggagg tgctggccca gttcccgcag
     3481 atcctggagg acccggcccc ttccatctgg atctccaagt acggtgccag cagcatcgaa
     3541 tatgtggtgc gggtgtggac cgccgcgggg gactactggg acgtgtatta cggcgttatg
     3601 gagggggtgc gccccgcttt ccagcgccac gggatccaga tgacctacga tcatctaaac
     3661 gtccacctgc tggagaagtg agccctcaga ccagccgggc gccccgtcca cggggccttc
     3721 agggcatggc cagcccgtgg agaaagccgt ccagctgggg agagaggacc tcgcccccga
     3781 tcaccgtgtt tttgtagatc agcagattgg ccaggatgcc ggtctcaccg gtggggtagt
     3841 tggtgatctc gtaggtgtag cgcttcaccc gcttgccggc atatttggac aggtcgaagc
     3901 cctgcccgct ttgcagggcc agatagtcgt catagctcgc atccatctcc ttggggatga
     3961 gcagctcctc ggtggccagg ggctcggcac tgacctccca gccgtaggac tggaggtagg
     4021 ccacccggtc ctcgttgctc ttgaccccct ttgggctggg gacggcagag gcggacgcct
     4081 cccgggcggg gccgccgccc agggtcagca ccagggcggc gcagcaggcc agggcggcca
     4141 cggccacccc gatggtcagc tttcggtggc ggggcagttt tgcagtcagg attatcacag
     4201 cgcattctcc ttccaacgcc ttggtttcta ctcatgtcta tgaggggcgg gacaaattta
     4261 tgcctgccct cataaagtcc ggacaggagc gcggagacgg ccggcaggca ggagggggcg
     4321 gaggaacagg aggacaagta tggcacagga gcgtctggac aagctgctct cctccacggg
     4381 gcggtggtcc cgccgggagg tgaaggaact gatccgccag gggcgggtgt gggtggacgg
     4441 cgtccctgcc cggcggccgg aggagaagtg tgagcccgga gcggccggcc tcactgtgga
     4501 cggagagcgg gtggactgcg cccctttcgt ctatctgatg ctccacaagc cggcgggcct
     4561 cctgtccgcc acggaggacc ggcggcagaa gacagtgctg gacctgctgc cggagcacct
     4621 gcgccgccgg gggctcttcc cggtggggcg gctggacaag gataccgagg ggctcctgct
     4681 cctcactgat gacggtcccc tcggccacgc cctgctctcc cccaaaaacc acgtggacaa
     4741 ggtgtactac gccaaggtgg aggggcggct ggaccggacg gacgctgagg ccctggcggc
     4801 ggggatggtg ctgggggacg ggctgcactg tctgcccgcg gagctggagc cgctggggga
     4861 cggctcccag tgtcatgtca ccctgcggga ggggaaatat caccaggtga agcggatgct
     4921 ggccgcccgg gggaagccgg tggtctatct gaagcgcctg tccatggggc ccatcgccct
     4981 ggacccggcg ctggcgccgg gggagtggcg gccgctgaca ggggaggaga aggcgctgct
     5041 gcggcgggga acagcgggag atccaggttt ttcggcgttt tgaacaaaaa attatgcaaa
     5101 acctcttgcc aaaaccgggg gggaaccgtt ataataggcg tagtgtacaa gtaacacatg
     5161 ggagggatca atgtgtccag caggatgttc cagggagtcg tactccagat gaaggacggc
     5221 atcgaccggg tggtgggtgt cattgacgcg gacggcatcg tcgtggcatg cagtgagctc
     5281 accagtatcg gtgagcattg gtccggcgcg gtggcggcca tcaatgccgc cgacaacggg
     5341 atcacccggt atgaggggaa gaccttcaag ccgctggccg gctggggcgc tcagttcgat
     5401 tacgccgctt ttgtccaggg ggaggacccg gagagcgcgg ccctgtgcgc catggcggtg
     5461 gtggccttca acggagccaa gacctcttat gaggagaagc acgacaaagc cacctttgtg
     5521 aaaaacatca tctcggacaa tatcctgctg ggggacatct atacccaggc taaggagctg
     5581 cactttgtct ctgaggtgcc ccgggcagct ttcctggtgc gccagctggg gcaggcggat
     5641 gtgagcacca tcgatgtgat ccagaacctg ttcccggaca agcagacaga ctttgtcatc
     5701 tccatcaatg agacagacgt ggccctgatc aagcagctgc ccgagggggc ggatgccaag
     5761 gagctccaga agatcgccaa gcagatctct accgcggtga accaggaact gggcatcaag
     5821 gtggtcattg gaatcggcac cgtggtcaac cacatccggg acctggcccg ggcctataag
     5881 gaggccggcg tagccatcga cgtgggcaag gtgttcgaca ccgagaagac ggtcatcaac
     5941 tatgagaacc tgggcatcgg ccggctgatc tatcagctgc ctaccatcct gtgccagatg
     6001 ttcctccagg aggtctttaa gaagaacccc atcgatgctt tggaccagga gactctgctc
     6061 accatcaaca agttctttga aaacaacctg aacgtgtccg agactgcccg gaagctgttc
     6121 gtccaccgca acaccctggt gtaccggctg gagaagatca agaagctcac cggcctggac
     6181 ctgcgggagt tcgatgatgc cattaccttc aaggtggcgc tgatggtcaa gaagtacctc
     6241 atttcccggg gaatcgaatc ctgaaccgaa gaacagcacg cccccgcgcc gggtccggcg
     6301 cgggggcgtt gattttttat gagaggagga aaaatttgga ggagcaaaat gccaaaattt
     6361 ttttctggga ggggttgccg ttccggggcg gcgccgctac aatataggag cggaagcgac
     6421 aaaatggccg caaaaatcca tttacataac acacaaactg tgatacactt tgagaaaagc
     6481 gcatcagccg cccgcccctg ggagagggcg tgccaaaggc acgacttggg ggagtggcgg
     6541 ccgtctggcg gagggacaca tgatacggtt catcgatgta tataaagaat acgacaacgg
     6601 gaccaaggcg ctgaaagggg tctccatgcg gatcgacgac ggggagttcg tctttctggt
     6661 ggggccatcc ggctccggga agtccaccat catcaagctg atcaccgggg agatcgccac
     6721 cacagagggg cggctgatgg tgaacggcta caacatgaac aacatccgtc cgggacaggt
     6781 gccctacatg cggcggacgc tgggcatcat tttccaggac ttccggctca ttgagaaaaa
     6841 gacggtgtat gagaacctgt cctttgccat gcgggccatc ggcacctcca cccgggagct
     6901 gcgccggcgc atcccctatg tgctggagct ggtggggctg gagaagaagg cggaccagta
     6961 tccgggggag ctgtccggcg gcgagcagca gcgggtggcc atcgcccggg ctctggtcaa
     7021 caatcccagc atgattatcg ccgacgagcc cacggggaac ctggaccccc agcgatccct
     7081 ggagatcatg atgctgctgg agcgcatcaa cgagctgggt accactctgc tggtggtcac
     7141 ccacgagaaa tccctggtca accggttctc caaacgagtc gtcgccatcg agaacggacg
     7201 gatcatcagc gacgagacag gcgggtatta caacaatggc gagacgattt aacggaaaat
     7261 actatctcag cgagggcttc cactctatct tcacccacgg cttcatgtcc tttgccgctg
     7321 tgtgcatgat cgtggcctgc ctgctgatca tgggttcctt caccctgctg gcggtcaacc
     7381 tggaccacat gctgggggac ctggaggacg agaatctgtt cctggcctac attgatgaca
     7441 gctactcgga ggagcaggcc cgggcccttc agccgaaaat cgaggctgtc cccaatgtgt
     7501 cccaggtcac ctttgtcaca agggcggagg cattggagga ttttaaggca gggcgggagc
     7561 agaacgattt gatggacagc ctgccccccg aggtgctccg ggatcggttc gagatccatg
     7621 tcaaggatat cgagcagatg gcggccaccg tgtctcaggt ggaggccgtc cggggagtgg
     7681 cccataccca ggcggccatt gagatcgccc agggcttcgt catggtgcgc aacatcgcca
     7741 ccggcgtggc ggctgtgctg gtggccatgc tggtggtggt ctccctgttc atcatcgcca
     7801 acaccatcaa gctggccacc ttctatcgca gggaggagat cgccatcatg aagatgtgcg
     7861 gtgcgaccaa cggcttcatc cgctggccct ttgtggtgga gggcatgatc ctgggcctta
     7921 caggcgccct ggtggctttc ttcatccaat gggggctgta tgaggcggtc tcccgactga
     7981 tcctccagac caacggcctg agcctgatca ccgttctgtc ctacgcctcc atggcgggga
     8041 acatcgtgct ggtcttctgc ggcgtgggac tggtgatcgg tgtgggcggc tcggtggtgg
     8101 ccatccggaa gttccttcag gtatgagata aggagaagca ggtatgagaa aaaagacaca
     8161 gaggatcgtg atggcagtgc tggcagggat catggccctg ctgatgctgc tgcccatcgt
     8221 agccaatatt ttcgtccact gaggggaggc ggcccatgaa aagacgcgct atccccgcca
     8281 tcagcctggc gctggtggtc ctcttttccg cctcctttct gctggctctg ccaggggcgg
     8341 tgtccatgcc ggcctccgcc gtgactcagg cggagatcga tgcgctgaag gagagcgccg
     8401 gcgagctgga gcagcagcaa aaggagatcc aggcccagtt aaacgccatc gccgccgaca
     8461 aggatgaggc attggaacag aagagcctgc tggagcagca gatcaacgtg atccagtcgg
     8521 agatcaacaa catcgacgca cagatcgcca aatatgacga gctgatctcc cagaaggagg
     8581 acgaactggc tcagctggag cagcaggagg cggagcagta tcagctcttc tgtgagcggg
     8641 tgcgctatat ggaggaggag ggggaggtct cctattgggc catcctcttt aacgcggagg
     8701 atttctctga catgctggac cgcttcatga tggtggagga gatcatggcc tatgacaatg
     8761 ccatcatgga cgagctgctg gccacccagg cccagatcga gcaggagaag gcggagctgg
     8821 aggaggctcg caccgcccag gaggaggccc ggcagaagca ggcggacgcc aaagccaggc
     8881 tgaagagcca gcaggcggag gtagacgccc tcatcagtca gatcagcagc caggaggacg
     8941 agttggaggc catggaggcc aagctccagg cggcggccaa ggccatggac aacgagatcg
     9001 cccggaaaga gcgggagctc caggcccagc tggctgccca gggcgtgacc atcgtcagtg
     9061 aaaagggctt caagtggccc agctatgcca ccaccattac ctccctgttt ggcagccgta
     9121 cgcacccggt caccgggcgg cccaacaacc acactggagt ggatatcgcc gccgccggcg
     9181 gaacggacat ctgggccgcc aagagcggcg tagtccttac ttccacctac aacagctcct
     9241 acggcaacta tgtggtcatc agccatgcca acggagagag caccctctac gcccatatga
     9301 gcaagcggct ggtcagtgag ggagataagg tgacccaggg acagaccatc ggcctggtgg
     9361 ggaccaccgg ttcctccacc ggaaaccacc tccactttga ggtacgcctg aacggacagc
     9421 gtcaggaccc cctgaactat tttagaggtt ccacctttac actgcgggcc agtggcaaga
     9481 gcgtgagcta cactgtgagc tgagagaggg gagcgcggca tggaagagat gcggtgtccc
     9541 tgcggcggga tctttcaaag cctggggatg gaatacctcc g
//
LOCUS       DBMDSJ010000016         9401 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2361_length_9401_cov_1.848495, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000016 DBMDSJ010000000
VERSION     DBMDSJ010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_9401_cov_1.848495"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(157..1491)
                     /locus_tag="ACZPAQ_00905"
     CDS             complement(157..1491)
                     /locus_tag="ACZPAQ_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014115934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR0902562.1"
                     /translation="MESYHFIFDVALILLVTKCFAMLTKRVDMPQVVGSLLAGLLLGP
                     SILNLVQPSDFLAMLSELGVVVLMFGAGLQTDIQELKKSGKAAFFIALIGVLVPLAGG
                     YVLAEVFNHGGSFLENMFVGTVFTATSVSISVETLKEMGKLSTRSGNAILGAALIDDI
                     LGLILLTLITSASDSSISLGIVMIKIVAFFVVTGVAGYFLHGLIQRWMNSASWNRKRF
                     AVISLAFCFFYAYVAEEVFGVADIIGAFFAGLMIANTTRAVYVNSQCETLSYMLLSPV
                     FFASVGLKVTLSSMDGTVILLTVIAIALAIVTKVIGCGLGAKLFGYTNAESLRIGVGM
                     VSRGEVALIVTNKGIATGMMNPIFLAPMVIMVAATTIVTPIMLRAVYPKKKAEEDYSD
                     LVQSDLVDNYEEVSDFDLAAQTMLDMHNQLQGKGRDGRSKGSGKPPRTPEQG"
     gene            complement(1852..2991)
                     /locus_tag="ACZPAQ_00910"
     CDS             complement(1852..2991)
                     /locus_tag="ACZPAQ_00910"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR0902563.1"
                     /translation="MKADPSRYLEFRRDIHRHPELSNQEVETTRRIREFLARDGLELC
                     PIGALNGGFVRINAGKARTLCMRADIDALPLTEHTGAPFSSVHEGVMHACGHDMHTAI
                     AAGIACELNRVKDRLNCNILVLFQPAEEANPVGGARPVTEAGFLTDQHIDEMYGLHMW
                     PSLPVGDIELRPGPIMAASDRFRIEVLGTAAHAAEPHLGVDAIVIGSEIDCALVQKLR
                     REVNPFEPVSVSIGSFQTFGRYNVVCDRAVLDGTVRTTSEESRNYLHRRIREIPQQIA
                     SLYGGQAKVDIQAGYGVLRNSPELFQRFSTHAARLLGQEHVHTDIHPSLIGEDFYYFS
                     HQVPSLYFHIGCQAEHPLHNDAFLPREEVLGVAIDLMTDYFLAQA"
     gene            complement(3043..3867)
                     /locus_tag="ACZPAQ_00915"
     CDS             complement(3043..3867)
                     /locus_tag="ACZPAQ_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012993.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HIR0902564.1"
                     /translation="MTNFALLQTNIAFCDPEANFKRVRELFAQAMAKDPRPDVVVLPE
                     DWSAGFSDEMFHHMEDFVEPENGPSVTVLRELARQYQVWVVGGSIATLHADGKMRNTT
                     FLIDRRGEIVGDYSKMHLYSDMDEHVPFANGDKTEVYDTELGRLGMMICYDIRFCELA
                     RTYALKKADVLVVTSDFPNPRVNHWRTLLTARAIENQMFVVACNRVGPSPMGTYCGHS
                     IIIDPWGDIIAEGGGEEEIITGSIDYTKTREVRDLIHMFRDRQPTLYGDTLLRPVD"
     gene            complement(3898..5199)
                     /locus_tag="ACZPAQ_00920"
     CDS             complement(3898..5199)
                     /locus_tag="ACZPAQ_00920"
                     /inference="COORDINATES: protein motif:HMM:NF015515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR0902565.1"
                     /translation="MIFGLHPLFGFLPLLLYIVLMMMGKDMNVSVLLCVILGAILTGE
                     SITGFADTLYASLGSFCALIGFIIVLGSGLAEVLSQTKVAHNLVYMVVNRFKLKSKKM
                     AILISMITSTLLVSLLGTLAGSNAIIAPILIPIVASVGLTPSTLGVILHGAGATGLYV
                     GPFVPPVVTITGLTGLSYGQYLITAGIPLAVIVWGSTFFMACRTQRKTEALEAYEESG
                     EDASAFVPDARCKRATLAFLLVMAVMLGYGLVVGAGASYAILVMMTASIVVGLVGGLS
                     FQSSLTAMIQGGSKMFWMFFMFVLFEPFLNYVSASGAFDAVCQLMQPLIDVGGEVAFL
                     MLSAAVGIFGISGTGVAQAQIIHELFLPMVQSLNIAMPIWGMVVLVGCQVTFFVTPTV
                     DMVGQMGLARSKNIKAMLANGWVITVITFAYVLIRAILYTM"
     gene            complement(5330..6187)
                     /locus_tag="ACZPAQ_00925"
     CDS             complement(5330..6187)
                     /locus_tag="ACZPAQ_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013539.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR0902566.1"
                     /translation="MEQTKDVVTQIRTHYDSFSKSHRRLADFILENLHEVAFLSINEL
                     SQRTGISPATITRFARRLDFQGYPDLQRGLYEHQKQWAPFGQLKSLLRRETPAEDAGP
                     DSLQWTIQNNIGLLEALYTPQLRDSFARAQEILHQARTIYIAGMRSSYATAYYLAFML
                     QQMCDNVHLLTTSTSDLPTALSDVRPEDCLLVISYARYTSSSYDIVSHFHRAGCKIVA
                     LTDSLTSPIALKATEVLIAPNGGNFSPVGAITLCNCFITSLGRLNAQQTLERMELQDK
                     IALEHHIYL"
     gene            complement(6323..6844)
                     /locus_tag="ACZPAQ_00930"
     CDS             complement(6323..6844)
                     /locus_tag="ACZPAQ_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902567.1"
                     /translation="MRPDPELRPDSLMQKIRSARQSDASFRRAHRTVQIIMASWAVIV
                     VLDRLIGLVSHSYTGSSLPFALAGGAVLVGIALLGARGHIQGALMVMQINMAVFLVQF
                     AATCFLYREHTAPWSTLFYGVSAGVLIAGSLMLFLRRDLEDYRAAVRRFKGKQDRAPR
                     FCRTNSRLIRIKK"
     gene            7169..>9401
                     /locus_tag="ACZPAQ_00935"
     CDS             7169..>9401
                     /locus_tag="ACZPAQ_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR0902568.1"
                     /translation="MRKLVIGILAHVDAGKTTLSEGMLYTCGTLKRLGRVDHQDAFLD
                     TEALERERGITIFSKQAVLPLPGVEVTLLDTPGHVDFSSEMERTLQVLDCAILVISGT
                     DGVQGHTRTLWRLLERYHIPTLLFVNKMDLAGADRAVLMAELQRLDSGCVDFGQEGPG
                     LQESVAMCDEGTLERYLERGQVEPEEIRALVEGRRLFPCWFGSALKLEGVAEFLEGVE
                     QYAPRKCCPPQFGARVYKVARDAQGVRLTYLKITGGSLRVKTPLTGGEGEMRWEEKAD
                     QIRIYSGAKYRAVEEAEAGTVCAVTGLSRTRPGEGLGFEAAGQPPVLEPVLTYRLELP
                     EGCDPHAALRQLRELEEEDPQLHILWDERLREIHIQLMGEVQLEILRQVIAQRFGVEV
                     SFGAGSIVYRETIAAPVEGVGHFEPLRHYAEVHLLLEPGERGSGLRVGTACSTDQLDL
                     NWQRLILTHLLEREHLGVLTGSPITDLRITLVAGRAHEKHTEGGDFRQATYRAVRQGL
                     MQAESILLEPWYDFRLELPASCLGRALSDIQRMGGEAAAPESQGEEAVLTGGAPVEGL
                     RDYGTQVAAYTRGQGRLFCTPGGYRPCGDQAGVVSALGYDPERDTDNPADSVFCSHGA
                     GVTVKWDQVRAHMHVDSGLRLGGEEAPPPETPVRPAERGPAGSLEQDKELMAIFERTY
                     GKVEQRAFQPQKKPARTSLDGEKYSIRTQKQGPEYLLVDGYNVIFAWEELERLARQDV
                     AAAR"
BASE COUNT         1985 a         2593 c         3228 g         1595 t
ORIGIN      
        1 cccctccttt cggggacggc agaccggcag gggactgcaa gacaagagat cggtgatatt
       61 gtatcacagg gactggagaa agcaagcggg agctaaagga gaaacagcgg gcctccggaa
      121 cgatcccgga ggcccgctgt tgaaaatgag atacggtcag ccctgctcag gggtgcgggg
      181 aggcttccca ctgcccttgg agcggccgtc ccggcccttg ccctggagct ggttgtgcat
      241 gtccagcatg gtctgggcgg ccaaatcgaa gtcgctgacc tcctcgtagt tgtccaccaa
      301 gtcgctctgt accaggtcgc tgtaatcctc ttcggccttc ttctttgggt agacggcgcg
      361 gagcatgatg ggggtgacga tggtggtggc agccaccatg atgaccatgg gggccaggaa
      421 gatggggttc atcattccag tggcgatccc cttgttggta acgatcaggg ctacctcgcc
      481 ccgggatacc atgccaacac cgatgcgcag ggactcggcg ttggtgtagc cgaacagctt
      541 ggcccccagg ccgcagccga tgaccttggt gacgatggcc agcgcgatgg cgatgacggt
      601 gagcaggatg acggtcccat ccatggagga gagggtgact ttcaggccca cactggcaaa
      661 aaagacgggg gagaggagca tgtaggagag ggtctcacac tgggagttga catagacggc
      721 ccgggtggtg ttggcgatca tcaggccggc gaagaaggcg ccgatgatat ccgccacacc
      781 gaagacctcc tccgccacat aggcatagaa gaagcagaaa gccagggaga tgacggcgaa
      841 gcgcttccgg ttccaggagg cagagttcat ccaccgctgg atcagcccgt ggaggaagta
      901 gccagccacg ccggtaacaa caaagaaagc cacgatcttg atcatcacga tgccaaggct
      961 gatgctgctg tcagaggcac tggtgatcag ggtcagcagg atcagcccca gaatatcatc
     1021 gatcagggca gcacccaaga tggcgttgcc ggaacgggtg gacagcttgc ccatctcctt
     1081 cagggtctct acactgatgg agacggaggt ggcggtgaat acggtgccca cgaacatgtt
     1141 ctccaggaag gaacccccgt ggttgaagac ctcggccagg acatagccgc ccgccagggg
     1201 gaccagcacg ccgatcaggg cgatgaaaaa ggcggctttg ccggattttt tcagctcctg
     1261 gatgtcggtc tggagtccgg cgccgaacat gagcaccacc acgcccagct cggagagcat
     1321 agcgaggaag tcactgggct ggaccaggtt gaggatgctg ggccccagca gcaggccggc
     1381 cagcagagag cctaccacct ggggcatatc cacccgtttg gtaagcatgg caaagcactt
     1441 tgtgaccagc aggatcaggg ccacatcaaa aataaaatga tatgattcca tggcagatta
     1501 ccttcttgca gacgaaatga tgcgtaactg tggcggctgc ggcggacgcc ctatggggaa
     1561 ttaagagaag acatcaatgc ccccagacgt ccggccgctg ggatcaaaga aagagcaacc
     1621 gtctccgtca gcgtatggat actaccacaa acaaaaaaga aagtccagtg atttgcaccg
     1681 agaaggtgta cgattttaac agaaactgaa caacaatttt gttatgaacg tcaaaagtga
     1741 gaaaatggaa gggaaagaag caaataaata aatgagagag gagatcgcac tagaaatgcc
     1801 gcgcccgggg tcacttccgg gcgcggcata agacagattt tcagaaaaag atcaggcctg
     1861 ggcgaggaag tagtcggtca tcagatcaat ggccacgccc agcacctcct cccggggaag
     1921 aaaagcgtcg ttgtgcaaag ggtgctcggc ctggcagcca atgtggaaat acagagaggg
     1981 gacctgatgg ctgaagtagt agaaatcctc tccgatcagg ctggggtgga tgtccgtgtg
     2041 gacgtgttcc tgtcccaaga gccgggccgc atgggtggaa aagcgctgga acagctccgg
     2101 tgagttgcgg aggacgccgt atccagcctg gatgtccacc ttggcctgcc cgccgtacag
     2161 ggaggcgatc tgctggggga tctcccgaat gcggcggtgg aggtagtttc ggctctcctc
     2221 actggtggtc cggaccgttc cgtccaggac ggcccggtca cagaccacat tgtagcgccc
     2281 aaaggtctgg aaggagccga tagagacgga gacgggctcg aagggattga cctcccgccg
     2341 gagcttctgg accagggcac agtcgatctc ggagccgatg acaatggcgt ccacccccag
     2401 gtggggctcc gccgcatggg ccgcagtgcc cagcacctcg atgcggaagc ggtcggaggc
     2461 cgccataatg gggccgggcc gcagctcgat gtcccccacc ggcagagagg gccacatatg
     2521 cagaccgtac atctcgtcga tgtgctggtc ggtgagaaaa ccggcctcag tgacagggcg
     2581 ggcgccgccc accgggtttg cctcctcggc gggctgaaac aggaccagaa tgttgcagtt
     2641 cagccgatcc ttcacccggt tcagctcaca ggcgatccca gcggcgatgg cggtgtgcat
     2701 atcgtggccg caggcgtgca tgactccctc gtggacagag gagaacggag cgccggtatg
     2761 ctcggtcagc ggcagggcgt cgatatccgc ccgcatgcac agggtcctgg cctttcctgc
     2821 gttgatgcgg acaaagccgc cgttcagcgc gccgatgggg cacagttcca gtccgtcccg
     2881 ggccagaaat tcccggatgc ggcgggtggt ctccacctcc tggttgctca gctccggatg
     2941 ccggtggata tcccggcgga actccagata gcgggaagga tcagctttca tacacaacct
     3001 tcttcctgaa aaagatgctg gggcaggccc cttttgggag gctcagtcca cggggcggag
     3061 cagcgtgtcc ccgtagagag tgggctgccg gtcccggaac atatggatca ggtcccggac
     3121 ctcccgggtc ttggtgtagt cgatggagcc ggtgatgatc tcctcctcac cgccgccctc
     3181 cgcgatgatg tcgccccagg ggtcgatgat gatggagtgg ccgcagtagg tccccatagg
     3241 gctggggccc acccggttgc aggccaccac gaacatctgg ttctcgatgg cgcgggcggt
     3301 cagaagggtg cgccagtggt tcaccctggg attgggaaag tcggaggtga ccaccagcac
     3361 atccgccttt ttcagggcgt aggtgcgggc cagttcgcag aagcggatat cataacagat
     3421 catcatgccc aggcggccca gctcggtgtc atagacctcg gtcttgtcac cgttggcgaa
     3481 ggggacatgc tcgtccatgt cgctgtacag atgcattttg ctgtaatcac ccacgatctc
     3541 cccccgccgg tcgatgagga aggtggtgtt gcgcatcttg ccgtcggcgt ggagggtggc
     3601 gatggagccg cccaccaccc agacctggta ctgccgggcc agctcccgca gcaccgtgac
     3661 agagggaccg ttttccggct ccacgaagtc ctccatgtgg tggaacatct catcagagaa
     3721 accggcggac cagtcctcag gcaggacgac cacgtcggga cgggggtcct tggccatagc
     3781 ctgggcaaac agctcccgga cccgcttgaa attggcctcg ggatcgcaga acgcgatgtt
     3841 ggtctgaagc agtgcaaagt tcgtcataca aacatctcca atctgatcaa atgccgttca
     3901 catggtgtac aaaatggcgc ggatgaggac ataggcgaag gtgatgacag tgatgaccca
     3961 gccgttggcc agcatggcct tgatgttctt ggagcgggcc aggcccatct gtcccaccat
     4021 atcaacggtg ggggtgacga agaaggtgac ctggcagccc accaggacca ccatgcccca
     4081 gataggcatg gcgatattga gggactgtac catggggagg aacagctcgt ggatgatctg
     4141 cgcctgagcc acgccggtgc cggagatgcc aaagatgccc actgcggcgc tgagcatgag
     4201 gaaggcgacc tcgccgccca catcgatcag gggctgcatg agctggcaga ccgcatcgaa
     4261 ggcgccggag gcgctgacat agttgaggaa gggctcgaac aggacgaaca tgaagaacat
     4321 ccagaacatt ttggagccgc cctgaatcat agccgtgagg gaggactgga aggacaggcc
     4381 gcccaccagg cctaccacga tagaggccgt catcatgacc aggatggcgt agctggcgcc
     4441 ggcgcccacc accaggccgt agcccagcat aacggccatc accagcagga aggccagggt
     4501 ggcgcgcttg cagcgggcat ccgggacaaa ggcggaagcg tcttcccccg actcctcata
     4561 ggcctccagc gcctcggtct tccgctgggt gcggcaggcc atgaagaagg tggagcccca
     4621 gacgatgacg gccagaggga tgccggcggt gatgaggtac tgtccgtaag agagcccggt
     4681 caggccggtg atggtgacca ccggggggac gaaggggcct acatacaggc cggtggctcc
     4741 ggcgccgtgg agaatgacgc ccagggtgga gggggtgagc cccacgctgg ccacaatggg
     4801 gatcaggata ggggcgatga tggcgttgga gccggccagg gtgcccagca gggagaccag
     4861 cagggtggag gtgatcatgg agatcagaat ggccatcttc ttgcttttca gcttgaagcg
     4921 gttgaccacc atgtacacca ggttatgtgc cacttttgtc tgggacagga cttccgccag
     4981 accagagccc aggacgatga tgaagccgat gagggcacag aaggagccca gggaggcgta
     5041 gagcgtgtcg gcgaagccgg taatggattc tccggtgagg atcgcgccca gaatcacgca
     5101 cagaaggaca gagacgttca tgtccttgcc catcatcatc aaaacgatgt agagcagcaa
     5161 gggcaggaag ccgaagaggg gatgcagccc aaagatcata gtttccatct cctttttttc
     5221 attcgcggtt tccgttggga caggcatatt atattcctgc accttccgga ggccggcttc
     5281 tgccggaggg cgcaaaaata ccggaagaaa aaggagcgga atcagatggt cagaggtaga
     5341 tgtggtgctc cagggcaatt ttgtcctgca gctccatccg ctccagggtc tgctgggcgt
     5401 tcagacggcc cagggaggtg ataaagcagt tgcacagggt gatggctccc acgggggaga
     5461 agttcccgcc gttgggggcg atcagcacct cggtggcttt cagcgcgatg ggggaggtga
     5521 gcgagtcggt caaagcgacg atcttgcagc ccgcccggtg gaagtgggat acgatgtcat
     5581 aggaggagct ggtgtaccgg gcataggaga tgaccagcag gcagtcctcc ggacggacat
     5641 cactgagcgc agtgggcagg tcgctggtgg aggtggtgag cagatggacg ttgtcacaca
     5701 tctgctggag catgaaggcc agatagtagg ccgtggcata ggaggaacgc atcccggcga
     5761 tatagatggt ccgggcctga tgcaggatct cctgggcgcg ggcaaaggaa tcccgcagct
     5821 ggggggtgta cagggcctcc agcaggccga tgttgttctg gatggtccac tgaagggagt
     5881 ccggtccggc gtcctcggca ggggtctccc ggcggagcag ggatttcagc tggccgaagg
     5941 gggcccactg cttctggtgc tcatagagcc cgcgctgaag gtccggatag ccctgaaaat
     6001 ccagcctgcg ggcaaagcgg gtgatggtgg ccggactgat cccggtccgc tggctgagct
     6061 cattgatgga gagaaaggcc acctcgtgga gattttccag aataaaatca gccagcctcc
     6121 ggtggctttt ggaaaaagaa tcgtagtggg tgcggatctg ggtgacaaca tcttttgtct
     6181 gttccatagt acagtttccc tctttacgat cataaaatgg cggacttggg gcggaaaagc
     6241 acagccccga cagaaccatt ataggaggaa accgccagaa aggaaactgt ttttcaacat
     6301 tttggcactc agataggcaa tcttattttt tgatgcggat gagccggctg ttggtgcggc
     6361 agaaacgggg agcgcggtcc tgcttcccct tgaagcggcg cactgcggcg cggtagtctt
     6421 ccagatcccg gcggagaaag agcatcaggg agccggcgat cagcacaccg gcggacaccc
     6481 cgtagaacag ggtggaccag ggggcggtgt gctccctgta cagaaaacag gtggcggcaa
     6541 actggaccag aaagacggcc atgttgatct gcatgaccat cagggcaccc tggatgtgtc
     6601 cacgggctcc caaaagggcg atgcccacca ggacggcccc gccggccagc gcaaagggga
     6661 gggagctccc tgtataggag tgggagacca gcccgatcag gcggtccagc actacgatga
     6721 ccgcccagga ggccatgatg atctgtacgg tgcggtgcgc ccggcggaag gaggcgtcag
     6781 actgtctggc gctgcggatc ttctgcatca gggagtcagg gcggagctca gggtcggggc
     6841 gcatagggat ccctccgttt tcagattcag taactgcatt ataacaaaat gaaaatatat
     6901 tttcaatata gaaaaagaaa tgaaaatata aaaccatttt tggagcatct gctgaaaatc
     6961 aggcgcgctt tggccaaaca gacctgaaag cggggaggtc tgaggaggtg cgttcaggca
     7021 cgatccggat gaaatgaggg aaagcgaagg ccgcatcggt ttttggcctt gatttgaggg
     7081 gctgaatcct ctaaaatggg aatatacaga acacagcggt ccggcggggc agtgacgccc
     7141 cgctgttttt gggaccggaa ggggaaccat gagaaagctg gtcattggaa ttttagccca
     7201 tgtggatgcg gggaagacca ccttatccga ggggatgctg tacacctgcg gcacactgaa
     7261 gcggctgggg cgggtggacc accaggacgc ctttctggac acggaggcgc tggagcggga
     7321 gcggggcatc accatttttt ccaagcaggc ggtgctgccg ctgcccggtg tagaggtcac
     7381 cctgctggac accccgggcc atgtggactt ctccagcgag atggagcgta ccctccaggt
     7441 gctggactgt gccatcctgg tcatcagcgg gacggacggg gtgcaggggc acacccggac
     7501 cctgtggcgg ctgctggagc gctatcacat ccccaccctc ctctttgtca acaagatgga
     7561 cctggcgggg gcggaccggg cggttctgat ggcagagctc cagaggctgg acagcggctg
     7621 cgtggacttc ggccaggagg gacccgggct ccaggagagc gtggccatgt gcgacgaggg
     7681 aacgctggag cgatatctgg agcgggggca ggtggagccg gaggagatcc gggccctggt
     7741 ggaggggcgc aggctgtttc cctgctggtt cggctccgcc ctgaagctgg agggggtggc
     7801 ggagttcctg gagggggtgg agcagtatgc cccccggaag tgctgtcccc cgcagttcgg
     7861 ggcccgggtg tacaaagtcg cccgggacgc ccaaggggtc cggctgacct acctgaagat
     7921 caccgggggc agcctgcggg tgaaaacgcc cctgaccggc ggggaggggg agatgcgctg
     7981 ggaagaaaaa gcggaccaga tccggatcta ctccggggcc aaataccggg ccgtggagga
     8041 ggcggaggcg gggaccgtct gcgctgtaac agggctgagc cgcaccaggc cgggagaggg
     8101 gctgggcttt gaggcggcgg gacagccccc ggtgctggag ccggtgctca cctaccggct
     8161 ggagctgccg gaggggtgcg atccccacgc ggctctccgg cagctgcggg agctggagga
     8221 ggaggacccc cagctccaca tcctgtggga cgagcgcctg cgagagatcc atatccagct
     8281 catgggggag gtgcagctgg agatcctccg ccaggtcatc gcccagcggt tcggcgtgga
     8341 ggtctccttc ggcgcgggga gcatcgtcta ccgggagacc atcgccgcgc cggtggaggg
     8401 agtgggccac tttgagcccc tgcgccacta cgccgaggtc cacctgctgc tggagcccgg
     8461 ggagcgtggc agcggcctgc gggtagggac cgcctgctcc accgaccagc tggacctgaa
     8521 ctggcagcgg ttgatcctca cccacctgct ggagcgtgag cacttggggg tgctcactgg
     8581 ctcccccatc acagatctga ggatcacact ggtggccggc cgggcccacg agaagcatac
     8641 cgaggggggc gacttccgcc aggccaccta ccgggcggtg cggcaggggc tgatgcaggc
     8701 ggagagcatc ctgctggagc cttggtacga cttccggctg gagctgcccg cctcctgcct
     8761 ggggcgggcc ctgtccgaca tccagcggat ggggggcgag gcggcggccc cagagagcca
     8821 gggggaggag gcagtgctca ccggcggggc cccggtggag gggctgcggg actacgggac
     8881 tcaggtggcc gcctatacca ggggccaggg ccggctgttc tgcacccctg ggggctaccg
     8941 accctgcgga gatcaggcgg gcgtggtatc cgccctgggc tatgacccgg agcgggacac
     9001 ggacaaccct gcggactcgg tgttctgttc ccacggcgcc ggcgttacag tgaagtggga
     9061 ccaggtgcgc gcccacatgc atgtggacag cggcctgcgc ctgggcgggg aggaggcgcc
     9121 tcctccagag acgcctgtcc gtccggcgga gcgggggccg gcgggctcac tggagcagga
     9181 caaggagctg atggccatct tcgagcgcac ctacggcaag gtggagcaga gggccttcca
     9241 gccccagaag aagcccgccc gcaccagcct ggatggggag aagtacagca tccggacaca
     9301 gaagcagggg ccggagtacc ttctggtgga tggatacaat gtgatctttg catgggagga
     9361 gctggagcgg ctggcccgcc aggatgtggc cgccgcccgg g
//
LOCUS       DBMDSJ010000017         9386 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2366_length_9386_cov_1.616694, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000017 DBMDSJ010000000
VERSION     DBMDSJ010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2366_length_9386_cov_1.616694"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="ACZPAQ_00940"
     CDS             <1..550
                     /locus_tag="ACZPAQ_00940"
                     /inference="COORDINATES: protein motif:HMM:NF025112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0902569.1"
                     /translation="RLAALLHDVGKPGCCTVDREGVGHFYGHAAAGAELADACLRRLR
                     LDNALRERVVTLIRRHDLQLEPEPRLVKRWLGRLGPELFFQWTALAKADAGAKFPDRA
                     PGAARWEQVEALARQILAERPCLTLRDLEADGNDALARGLRGPAVGRALAALLEEVME
                     GRLPNHRAELMAELDRLAGRER"
     gene            complement(618..1412)
                     /locus_tag="ACZPAQ_00945"
     CDS             complement(618..1412)
                     /locus_tag="ACZPAQ_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR0902570.1"
                     /translation="MLIRELTFAAIGTGFTCLATVLGAAMVFFFREDLKPATQKIFLG
                     FAAGVMVAASVWSLLIPSMEMAEEMGQSPALPVGGGFLLGGAFLMVLDRLTPHLHIGS
                     SDPEGLPAHLSRTTMIVLAVTLHNIPEGMAVGLAFSMAAQDTGSGALAGAVALAIGMG
                     LQNFPEGAAISLPLRSDGVGTGRAFAAGALSGVVEPIFGLLTVLVAGSVSLVMPWVLS
                     FAAGAMIYVVVEELIPEAHLGEHSHVGTASVMCGFLVMMLLDVMLG"
     gene            complement(1500..2459)
                     /locus_tag="ACZPAQ_00950"
     CDS             complement(1500..2459)
                     /locus_tag="ACZPAQ_00950"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR0902571.1"
                     /translation="MRCFFAPMEGITGALFRRTHRRFFPGVDRYFMPFLSPSQHHVFT
                     RRELRDILPEQDGDLDAVPQLLTRSGADFLWAAGELARMGYREVNLNLGCPSGTVTAK
                     GKGAGMLGRPEELDHLLEDIFSASPTAVSVKTRLGIQDPEEFWPILDIYNKYPIAQLI
                     VHTRVREDLYRRPARPELFPAILAASRTPLCYNGDLVTAADCRAFSVRFPGAGLMMGR
                     GLVADPALASKAKGGPGADRDTLRAFHDALYEGYARDFGSRRNAMLRMKELWSYLIHL
                     FRDPESYAKKLRKAADPAEFEALTAGMFRELELLPGLEPGFLF"
     gene            complement(2469..2957)
                     /gene="bilS"
                     /locus_tag="ACZPAQ_00955"
     CDS             complement(2469..2957)
                     /gene="bilS"
                     /locus_tag="ACZPAQ_00955"
                     /inference="COORDINATES: protein motif:HMM:NF045594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HIR0902572.1"
                     /translation="MKQIILYDSATGNTEYLAKALSHALPNVPCGPVGSLDCSKAELV
                     YLGFWTDKGTCSDRLRAVLPSLAGKQIFLFGTAGFGADQAYFSQIIRRLEGELPQNCT
                     VIGWYMCPGRMGEGVRRRYEAMLQDPVSAGQAELLLKNFDAVRTRPDERDAQALLAAA
                     GL"
     gene            3267..5555
                     /locus_tag="ACZPAQ_00960"
     CDS             3267..5555
                     /locus_tag="ACZPAQ_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012613.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR0902573.1"
                     /translation="MKTGLRRLLALALGAGLLTANTYAAATTFTDVPTTYWGYYYIDR
                     AATEGLVSGIGDNQYGPEQTLSNAQFVTMICNMFYKTAVAANQNSSGQWWYPYMAAAY
                     SAGILSNTTVAQRRAAAGGWTEAMVNAEISRYDMAQIMVNVAGSKGWENPSALDLMAA
                     RISIKDWSTIPSNYQNAVAGAYARKLLSGDENGNFNGAASTTRAQAAVVLCSLYDAST
                     TIQVPTYVNSNRLVSGKTPSESNVYDDLVVLKSDYPENYLWDMTRSYTSKELGTATGV
                     EAFAYMLSDKVFGAMAASKLKDPADLRVGDVVSFDSGSDFGVVVSVDRNDFTYVTCDN
                     SGWITWKETADLDDLDSRDTVYTRYLTKASGDDTLSNGEKATESNVEDLLDDLMDDYE
                     DGDDWDMSDRYTSNVFGSAKGDEAFAYTISDEIFDELDYKSVKYAEDLRAGDVIYDSW
                     NELWGVVVDVDTKNEVCTYGSIDSKDRIAWDFEADFDDLDEMYTRYPSSSSSSSDDEL
                     TNGDSITESHVEDYLWEFEDDRDYGDGEYWDDDDEDFAWSLSDEIFGDVDYTKHSKTS
                     KLKIGDIIYDDDEGLYGVVIDIDEDRERAYYVSVYSDGEIDWNFWTYFDDITTIYTRY
                     PDSTSSDDTLSNGKKVNESNVEALLEKKLKDIDSWDMKEYYDSKVFGDDLSGAEGFAW
                     MLSDKVFGNLDYERLSDKKELQAGDVIEFKDDDSYGVVTDSQGDSDYDFFYVTVNSKG
                     RIQEDQRADYEDIYRVYTRYPD"
     gene            5887..6396
                     /locus_tag="ACZPAQ_00965"
     CDS             5887..6396
                     /locus_tag="ACZPAQ_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR0902574.1"
                     /translation="MVHLYCGEGKGKTTAAMGLALRMAGRGKRVVVAQFLKGADSGER
                     YALAMLPQVTLLPLPERVKFSFQLTPEEREAEQVRYLALAEQARTLGAQPDCGLLVLD
                     EVCAAVNTGLLPLEEVISCLDRAAGEVVLTGRDPDPALVGRADYITEMGKLRHPFDLG
                     TAARPGVEY"
     gene            6615..9242
                     /gene="ppdK"
                     /locus_tag="ACZPAQ_00970"
     CDS             6615..9242
                     /gene="ppdK"
                     /locus_tag="ACZPAQ_00970"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR0902575.1"
                     /translation="MATKYVYLFSEGNANMRELLGGKGANLAEMTNIGLPVPQGFTIT
                     TEACTQYYEDGQKINDEIQAQIMEYIVKMEEITGKKFGDLDNPLLVSVRSGARASMPG
                     MMDTILNLGLNEQVVEVMAQKSNNPRWAWDCYRRFIQMYSDVVMEVGKKYFEQLIDKM
                     KEERGVTQDVELTADDLKELAGQFKAEYKAKIGVDFPTDPKEQLMGAIKAVFRSWDNP
                     RANVYRRDNDIPYSWGTAVNVQSMAFGNMGDDCGTGVAFTRNPATGEKKLFGEFLTNA
                     QGEDVVAGVRTPMPIAEMAEKFPEAFAQFEGVCKTLEDHYRDMQDMEFTVEHGKLFML
                     QTRNGKRTPAAALKIACDLVDEGMIDEKKAVAMIEPRSLDTLLHPQFDAEVLKKTPVI
                     GSALAASPGAASGKIVFTAEDAKEWAARGEKVVLVRLETSPEDIEGMKAAQGILTVRG
                     GMTSHAAVVARGMGTCCVSGCSAIVMDEENKQFQLAGKTFHEGDWLSLDGSTGKIYDG
                     AIPTVDASIGGEFGRVMAWCDKYRRLQVRTNADTPHDAAQARKFGAQGIGLCRTEHMF
                     FDEDRIPAIREMICSDTVEQREKALAKLEPMQQSDFEGIYEAMEGCPVTIRFLDPPLH
                     EFVPTEEADIEKLANDMGKTVADIKAIIAGLHEFNPMMGHRGCRLAVTYPEIAAMQTR
                     AVIKAALNIQAKHPEWNMVPEIMIPLVGEVKELAYVKKVVTETADAIIASVKSDMKYK
                     VGTMIEIPRAAITADEIAKQADFFSFGTNDLTQMTFGFSRDDAGKFLDAYYDRKIYEN
                     DPFAKLDQKGVGRLIEMAAKLGRTANPDLHLGICGEHGGDPSSVEFCHRVGLDYVSCS
                     PFRVPIARLAAAQAAIKEG"
BASE COUNT         1955 a         2845 c         2984 g         1602 t
ORIGIN      
        1 gcggctggcg gccctgctcc acgacgtggg gaagccgggg tgctgtaccg tggaccggga
       61 gggggtgggc cacttttacg gacacgccgc cgccggggcg gagctggcgg acgcctgcct
      121 gcgccgcctg cggctggaca atgccctgcg ggagcgggtg gtcaccctga tccggcgcca
      181 cgacctccag ctggagccgg agccccggct ggtgaagcgg tggctggggc ggctggggcc
      241 ggagctcttc ttccagtgga ccgccctggc caaagcggac gccggggcca aatttccgga
      301 ccgggctccg ggggcagccc gctgggagca ggtggaggca ttggcaagac agatcctggc
      361 cgagaggccc tgcctcaccc tgcgggacct ggaggcggac ggaaacgacg ccctggcccg
      421 gggcctgcgg gggcccgcgg tgggccgggc gctggccgcc ctgctggaag aggtaatgga
      481 gggccgtctt cccaaccacc gggccgagct gatggccgag ctggaccgtt tggcggggag
      541 ggagcgctga gcgctccgcc gcccgctggg ggcacaaaac aggaccgggg gacagcgcgg
      601 gctgtccccc ggggcattta tcccagcatc acatccagca gcatcatcac caggaagccg
      661 cacatgacgc tggcggtgcc cacatgggag tgctccccca ggtgggcctc cgggatcagt
      721 tcctccacca ccacatagat catggccccc gcggcgaagg aaaggaccca gggcatcacc
      781 agggagacgc tgcccgccac cagtacagtg agcaggccga agatgggctc taccacgccg
      841 gacagggcgc ccgcagcgaa ggccctgccg gtgcccacgc cgtcggaccg gagggggagg
      901 gagatggcgg ccccctccgg gaagttctgg agccccatcc cgatggccag ggccactgcc
      961 ccggccaggg cgccgctgcc ggtgtcctgg gcggccatgg agaaggccag ccccaccgcc
     1021 atcccctccg ggatgttgtg cagggtgacg gccagtacga tcatggtggt gcggctcaga
     1081 tgggcgggca gcccttccgg atcgctgctg ccgatgtgga gatggggggt gagccggtcc
     1141 aggaccatga ggaaagcccc gcccagcagg aacccgccgc ccaccggaag ggcgggggac
     1201 tgccccatct cctccgccat ttccatagag gggatgagca gggaccacac agaggcagcc
     1261 accatcacgc cggctgcgaa gcccagaaag atcttctggg tggcgggttt caggtcctcc
     1321 cggaagaaaa acaccatggc ggctcccagc acggtggcca gacaggtaaa gccggtgccg
     1381 atggcggcaa aggtaagttc tcggatcagc atagcgacct cctgccccag ggactggggc
     1441 acgttcagtt tatgtagagc ggggccattc tatttgggcg tccccgctcc tggggcgggt
     1501 cagaacagaa aaccgggctc caggccgggc aggagctcca gctcccggaa catcccggca
     1561 gtgagggcct caaactccgc cgggtccgcc gccttgcgca gctttttggc atagctttcc
     1621 gggtcccgga agaggtggat gagataggac cagagctcct tcatccgcag catggcgttg
     1681 cgccggctgc cgaagtcacg ggcatagccc tcatagaggg cgtcgtggaa cgcgcggagg
     1741 gtgtcccggt ccgcgccggg cccgcccttg gccttggagg ccagcgccgg gtcggccacc
     1801 agccccctgc ccatcatcag acctgcgccg gggaagcgga cagagaaagc tcggcagtcc
     1861 gccgctgtca ccaggtctcc gttgtagcac aggggggtgc ggctggcggc caggatggcg
     1921 gggaacagct ccggccgggc ggggcggcgg tacaggtcct cccgcacccg ggtgtggaca
     1981 atgagctggg cgatgggata cttgttgtag atgtccagga tgggccagaa ctcctccggg
     2041 tcctggatac ccagacgggt cttgacagag acggcagtgg gggaggcgga gaagatgtcc
     2101 tccagcaggt ggtccagttc ctccggccgc cccagcatcc cggccccctt ccccttggcg
     2161 gtgacggtgc cggaggggca gcccaggttc aggttgacct cccggtagcc catccgggcc
     2221 agctcacctg ccgcccagag aaagtccgcc ccgctccggg tgagcagctg gggcaccgca
     2281 tccaggtccc cgtcctgctc cggcaggatg tcccgcagct cccggcgggt gaacacgtgg
     2341 tgctggctgg gggagagaaa gggcatgaaa taccggtcca cccccgggaa gaaccggcgg
     2401 tgggtccggc ggaacagggc tcctgtgatc ccctccatgg gggcgaaaaa gcagcgcata
     2461 gcctgcaatt acagacccgc cgcagccagc agggcctgag catctctctc atcagggcgg
     2521 gtgcggaccg cgtcgaagtt cttcagcaga agctctgcct gtccggcgga gacggggtcc
     2581 tgaagcatgg cctcatagcg gcggcgcact ccctctccca tgcgcccagg acacatgtac
     2641 cagccaatca cggtgcagtt ctgggggagt tcaccctcaa ggcggcggat gatctgagaa
     2701 aaatacgcct gatccgcacc aaagccggcg gtgccgaaga gaaagatctg cttgcctgcc
     2761 agtgagggga gaacagcccg cagacggtcg gagcaggtgc ccttgtccgt ccagaaaccc
     2821 agatagacca gctcggcttt cgagcagtcc agggacccga ctggaccgca tgggacgttg
     2881 ggcagggcat gggagagggc tttggccaga tattctgtgt ttcctgtggc actgtcatat
     2941 aaaatgatct gcttcatcat cggactcctt tttattctaa gcaataggga ctgatcctta
     3001 tggtgattat agcatcccgg cggggctctt gtcgacagca agagaaggaa aatcatagcg
     3061 gtagggaggg aaaatgttca tggtaaaatc ttcacaaaat gaaggaggac aaaaattaaa
     3121 atttcataaa tatgcaaggt atgttttgac ttttggacaa aaacacagta aaataaaaag
     3181 ggggcttcta cccgattttc cgcggcacgg gatggccgcc cccagaattt taggatgaca
     3241 aaacgcagga aaaggagaat agaatgatga agacaggttt gcggcgcctg ttggcgctgg
     3301 ctctgggagc ggggctgctg accgcgaaca cctacgcggc cgccactaca ttcaccgatg
     3361 tgcccaccac ctactggggc tattattaca ttgaccgggc cgccacagag gggctggtgt
     3421 ccggcatcgg agacaaccag tatggaccgg agcagaccct gtccaacgcc cagttcgtca
     3481 ccatgatctg caacatgttc tataagacgg cggtggccgc caaccagaac agctccggcc
     3541 agtggtggta tccctacatg gccgcagcct actcggcggg catcctgagc aacaccactg
     3601 ttgcccagcg ccgcgccgcc gccggcggct ggaccgaggc catggtgaac gcagagatca
     3661 gccgctatga catggcccag atcatggtca acgtggccgg ctccaagggg tgggagaacc
     3721 ccagcgcact ggatctgatg gccgcccgga tcagcatcaa ggactggagc accatcccca
     3781 gcaattacca gaatgctgtg gccggggctt atgccagaaa gctgctcagc ggcgatgaga
     3841 acggtaactt caacggcgcc gccagcacca cccgtgccca ggcagctgtg gtgctctgct
     3901 ccctgtatga cgcctccacc accatccagg tgcccaccta cgtcaactcc aaccggctgg
     3961 tgagcggcaa gaccccctcc gagtccaacg tgtatgacga cctggtggtc ctgaagagcg
     4021 actatcccga gaactacctg tgggatatga cccgcagcta cacctccaag gaactgggca
     4081 ccgccaccgg cgtggaggcc tttgcctaca tgctcagcga caaggtgttc ggcgccatgg
     4141 ccgccagcaa gctgaaggac cccgcggacc tgcgggtggg cgacgtggtc agcttcgact
     4201 ccggcagcga cttcggcgtg gtggtctctg tggaccggaa tgacttcacc tatgtcacct
     4261 gtgacaacag cggctggatc acctggaagg agaccgcgga cctggacgac ctggacagca
     4321 gggatactgt ctacacccgc tatctcacca aggcctccgg cgacgacacc ctgagcaacg
     4381 gagaaaaggc cactgagtcc aatgtggagg accttctgga cgacctgatg gacgactatg
     4441 aagacggcga cgactgggat atgagcgacc gctacacctc caacgtgttc ggctccgcca
     4501 agggcgacga ggcctttgct tacaccatca gcgacgagat ctttgacgag ctggattaca
     4561 agagcgtgaa atatgccgag gacctgcggg caggcgacgt gatttatgac agctggaatg
     4621 agctgtgggg tgtcgtggtg gatgtggaca ccaagaacga agtctgcacc tacggcagca
     4681 ttgattccaa ggacaggatc gcatgggatt tcgaggctga tttcgatgac ctggacgaga
     4741 tgtacacccg ctatcccagc agctcttctt catcctctga cgatgaactg accaacgggg
     4801 acagtattac cgagtctcat gtggaagatt atctgtggga gtttgaggac gacagggatt
     4861 atggcgatgg agaatactgg gatgacgatg atgaagactt tgcctggagc ctgagcgacg
     4921 agatcttcgg tgatgtggac tatacgaagc acagcaagac cagtaagctg aagattggtg
     4981 atatcatcta tgatgacgac gaaggactgt atggcgtggt catagatatc gatgaagacc
     5041 gtgaacgggc ctactatgtg agcgtgtact ctgacggcga aatcgactgg aatttctgga
     5101 cctattttga cgatatcact acgatttata cccgttatcc cgacagcacc tcttctgacg
     5161 acaccttgag caatggaaaa aaggttaatg agagcaatgt agaggccctt ttggaaaaga
     5221 agctgaagga tattgactcc tgggacatga aggaatacta tgattccaaa gtgtttggag
     5281 atgatctgtc tggagccgaa gggtttgcct ggatgctcag tgataaagta tttggaaatt
     5341 tggactatga gcgtttgagc gacaaaaagg agctccaggc aggggatgtg atcgagttca
     5401 aagacgatga ttcctatggt gtcgtgacgg attcgcaggg agatagtgac tacgatttct
     5461 tttatgtgac tgtaaacagt aaaggaagaa ttcaggaaga ccaaagggca gattacgaag
     5521 atatttaccg cgtctacacc cgctaccccg actgatacgc gaacgacaga cctgacctca
     5581 caggcgcgcg gctcaaaaag ccgcgcgcct gtttcatctc cttcccgtcc atcttcacgc
     5641 cccatctcag gggcgtccct ccacacgggc caataggccg ggcgagcctg ggacctgccc
     5701 ctgcatggga gctgggaaca aatatacccc acatggtgcg catgccctga tcccgatgta
     5761 ggggcgggct ggccccgccg gccccaaagg tatcccgccc tgtcaccagc tttcctcaaa
     5821 aaagaggtat atttttttcc aaaaagctgg tagaataaac cgaaaacata cacaaggagt
     5881 gagcttatgg tccatcttta ctgcggagag ggcaagggta agactactgc cgccatgggg
     5941 ctggccctgc ggatggcggg ccggggaaag cgggtggtgg tggcccagtt cctaaagggg
     6001 gcggacagcg gcgagcgtta tgccctggcc atgctgcctc aggtcaccct gctccccctg
     6061 ccggagcggg tgaaattctc ctttcagctc accccggagg agcgggaggc ggagcaggtc
     6121 cgctatctgg ccttggcgga gcaggcccgg acactggggg cacagccgga ctgcggactg
     6181 ctggttctgg acgaggtctg tgctgcggtg aacaccggcc tgctcccact ggaagaggtg
     6241 atctcctgcc tggaccgtgc ggcgggggaa gtggtcctca ccgggcggga ccccgacccg
     6301 gccctggtgg gacgggcgga ctacatcact gagatgggca agctgcgcca tccctttgac
     6361 ctggggacgg cggcccggcc tggggtggag tactgagggc aacatcgcca taaatgtgaa
     6421 aaaatcgtga aaaactcccg ccggacagtt gcaatatttc gtgcaaatag gtataatatt
     6481 tccgcgactg tcaaaggagg cctgtggggg cccccggagg gcggcggcga cttgggcttg
     6541 tttgagacat gccggtacgt ccggtgccgc cgtgtttcag acaagcccca ggattgaaga
     6601 aaggaatgaa gtaaatggca acaaagtatg tgtacctctt ctctgagggc aacgccaaca
     6661 tgcgtgagct gctgggaggc aagggcgcca acctggccga gatgaccaac atcggcctgc
     6721 ccgtgcccca gggcttcacc atcaccaccg aggcgtgcac tcagtactat gaggacggcc
     6781 agaagatcaa cgacgagatc caggcgcaga tcatggagta tatcgtcaag atggaggaga
     6841 tcaccggcaa gaagttcggc gatctggaca accccctgct ggtctctgtc cgttccggcg
     6901 cccgggcctc catgcccggc atgatggaca ccatcctcaa cctgggcctg aacgagcagg
     6961 tggtggaggt catggcccag aagtccaaca acccccgctg ggcctgggac tgctaccgcc
     7021 gctttatcca gatgtactcc gacgtggtca tggaggtggg caagaagtac tttgagcagc
     7081 tcatcgacaa gatgaaggag gagcgcggcg tcacccagga cgtggagctc accgctgatg
     7141 acctgaagga gctggccggc cagttcaagg ccgagtacaa ggccaagatc ggcgtggact
     7201 tccccaccga ccccaaggag cagctgatgg gcgccatcaa ggccgtgttc cgctcctggg
     7261 acaacccccg cgccaacgtc taccgccggg acaacgacat cccctactcc tggggcaccg
     7321 ccgtcaacgt gcagtccatg gctttcggca acatgggtga cgactgcggc accggcgtgg
     7381 ctttcacccg caaccccgcc accggcgaga agaagctgtt cggtgagttc ctgaccaacg
     7441 cccagggcga ggacgtggtg gccggtgtcc ggacccccat gcccatcgcg gagatggccg
     7501 agaagttccc cgaggccttt gcccagttcg agggcgtctg caagaccctg gaggaccact
     7561 atcgggatat gcaggacatg gagttcaccg tggagcacgg caagctcttc atgctccaga
     7621 cccgcaacgg caagcgcacc cccgccgcag ccctgaagat cgcctgcgac ctagtggacg
     7681 agggcatgat cgacgagaag aaggccgtgg ccatgatcga gccccgctct ctggacaccc
     7741 tgctccaccc ccagtttgac gccgaggtcc tgaagaagac tcccgtcatc ggctctgctc
     7801 tggccgcctc ccccggcgcc gcctccggca agatcgtctt caccgctgag gacgccaagg
     7861 agtgggccgc ccggggcgag aaggtggtcc tggtccgcct ggagacctct cccgaggata
     7921 tcgagggcat gaaggctgcc cagggcatcc tcaccgtccg cggcggcatg acctctcacg
     7981 ccgccgtcgt ggcccgcggc atgggcacct gctgcgtgtc cggctgctcc gccatcgtta
     8041 tggatgagga gaacaagcag ttccagctcg ccggcaagac cttccacgag ggcgactggc
     8101 tgagcttgga cggctctacc ggcaagatct atgacggcgc catccccact gtggatgcct
     8161 ccatcggcgg cgagttcggc cgggtcatgg cctggtgcga caagtaccgc cgcctccagg
     8221 tccgcaccaa cgccgacacc ccccacgacg ccgcccaggc ccgcaagttc ggcgcccagg
     8281 gcatcggcct gtgccgcacc gagcacatgt tcttcgacga ggaccgcatc cccgccatcc
     8341 gggagatgat ctgctccgac accgtggagc agcgcgagaa ggctctggcc aagctggagc
     8401 ccatgcagca gagcgacttc gagggcatct atgaggctat ggagggctgc cccgtcacca
     8461 tccgcttcct ggaccccccg ctccacgagt tcgtgcccac cgaggaggct gacatcgaga
     8521 agctggccaa cgacatgggc aagactgtcg ctgacatcaa ggctatcatc gccggcctgc
     8581 acgagttcaa ccccatgatg ggccaccggg gctgccgtct ggccgtgacc taccccgaga
     8641 tcgccgccat gcagacccgc gccgtcatca aggcagcgct gaatatccag gccaagcacc
     8701 ctgagtggaa catggtcccc gagatcatga tccccctggt gggcgaggtc aaggaactgg
     8761 cctatgtgaa gaaggtggtc accgagaccg ccgacgccat catcgcttct gtcaagtccg
     8821 acatgaagta taaggtgggc accatgatcg agatcccccg tgccgctatc accgctgacg
     8881 agatcgccaa gcaggccgac ttcttctcct ttggcaccaa cgacctgact cagatgacct
     8941 tcggcttcag ccgtgacgac gcgggcaagt tcctggatgc ctactacgat aggaagatct
     9001 atgagaacga tccctttgcc aagctggacc agaagggcgt gggccgtctg atcgagatgg
     9061 ctgccaagct gggccgtacc gctaaccccg acctgcatct gggcatctgc ggtgagcacg
     9121 gcggcgatcc ctcctctgtg gagttctgcc accgtgtggg cctggactat gtttcctgct
     9181 cccccttccg tgtgcccatc gcccgtctgg ccgccgcgca ggctgccatc aaagagggct
     9241 gatcccaagc gatcctctgt gaaaaaagcg ccccgctcat atgagcgggg cgcttttttc
     9301 agctcaaagg cgggagagag cacagggagt gcctcagaac aggccgagga tcaggatggt
     9361 gtaccgcttc aggtccagca tcccgc
//
LOCUS       DBMDSJ010000018         9116 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2427_length_9116_cov_1.834165, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000018 DBMDSJ010000000
VERSION     DBMDSJ010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2427_length_9116_cov_1.834165"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            141..1076
                     /gene="fabD"
                     /locus_tag="ACZPAQ_00975"
     CDS             141..1076
                     /gene="fabD"
                     /locus_tag="ACZPAQ_00975"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR0902576.1"
                     /translation="MKLAFLYAGQGTQHPGMGRDLYETYPAFRQVLDSAQLDFDLKEL
                     CFEGPEEKLSQTQYTQPCMVAFAAGVTAVLREKGIIPEVAAGLSLGEYSALQAAGVFD
                     SKTAVELVAFRGKAMAQAVQDRPCGMAAVLMLGREQLAECCRQAGEAGVVEIANYNCP
                     TQLVIAGDAAAVEKAGELALQAGAKRVVPLKVSGPFHTSLMAPAGEALAEKFRSVAFG
                     EMSFPVLFNCKGGPMEAGETIPELLVRQVQSSVYLEDTIRAMAGMGVDTIVEIGPGKA
                     LSAFVKKTEKGIKTYAVETVEDIEKLCEALKGEQA"
     gene            1073..1816
                     /gene="fabG"
                     /locus_tag="ACZPAQ_00980"
     CDS             1073..1816
                     /gene="fabG"
                     /locus_tag="ACZPAQ_00980"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR0902577.1"
                     /translation="MSLKGKVALVTGGSRGIGRAVCLELARQGAYVAVNYAGNEAAAQ
                     ETAQACRELGVRAVVLRADVADAAACDAMVAQVLEQLGRLDILVNNAGITRDGLMLRM
                     PEADWDAVLDTNLKGAFHCMRAAYKTMMRQKSGRVVNISSVVGLRGNAGQTNYSASKA
                     GLIGLSKSLAKELATRNVTVNVVAPGFITTDMTDALPEGAREALLSTIPMKRLGAAED
                     VARAVAFFAGEGAGYVTGQVLCVDGGMAV"
     gene            1837..3078
                     /gene="fabF"
                     /locus_tag="ACZPAQ_00985"
     CDS             1837..3078
                     /gene="fabF"
                     /locus_tag="ACZPAQ_00985"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR0902578.1"
                     /translation="MEKRRVVITGLGAVTPLGHTARETWEAAKAGVCGIAPITLYDHS
                     AQKVSLAGEVKEFDPSGVLEKKEIRRMDRFAQFAVTAAAEAMAHSGLDLEREDRARIG
                     VALSSGIGGIGTLQTSCETGQSRGFDRVSPFFIPMVITNLAAGHIAIRFGLHGMCICP
                     VAACAGGANAVGDAFRHIRDGYAEVMVAGGAEASITPLAMGGFTSMSALTDASDPSRA
                     SIPFDKERSGFVMGEGAAVLILEELEHARARGAQILGEITGYGATCDAYHITAPDPEG
                     AWSAACMEQALADAGLAPEAVDYINAHGTSTPLNDSGETAAIRRVFGAHADRLMVSST
                     KSMTGHLLGASGAVEAALCVLALKDGFVPATINYQVPDPACDLDIVPNEGREADIRCA
                     ISNSLGFGGHNACLVFQKWEG"
     gene            3082..3522
                     /gene="fabZ"
                     /locus_tag="ACZPAQ_00990"
     CDS             3082..3522
                     /gene="fabZ"
                     /locus_tag="ACZPAQ_00990"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR0902579.1"
                     /translation="MELDANQIQEILPHRPPFLLVDRITGGEPGVWAQGIKCVTVNEP
                     FFAGHFPGYHVMPGVLIMEALAQVGAVAILSLPENKGKLAFFGGIKNARFKRQVVPGD
                     VLELRCELTRQVGSVGFGKAEARVNGKLAAQGELTFAVAQPGDP"
     gene            3572..4039
                     /locus_tag="ACZPAQ_00995"
     CDS             3572..4039
                     /locus_tag="ACZPAQ_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0902580.1"
                     /translation="MLEKDFNEVYTKFKLHFYRAVFGRFKRREATLTTVETFCMEIIL
                     ALGEPTVNEFSNFVGISPPNAAYKINSLVQKGYVEKRQSQEDKREYHLVATQKYLDYY
                     NISYHYLNTVMSRIRARFSPEDVAKLEGMLEIISRELMPEIPLGDQIHQAEQA"
     gene            complement(4090..4335)
                     /gene="rpsR"
                     /locus_tag="ACZPAQ_01000"
     CDS             complement(4090..4335)
                     /gene="rpsR"
                     /locus_tag="ACZPAQ_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR0902581.1"
                     /translation="MPMDRDNRGPRGRKRRKVCAFCVDKVECIDYKDAAKLRRYISER
                     AKILPRRTTGTCAMHQRQLTEAIKRARQVALLPYVTD"
     gene            complement(4390..4857)
                     /locus_tag="ACZPAQ_01005"
     CDS             complement(4390..4857)
                     /locus_tag="ACZPAQ_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR0902582.1"
                     /translation="MLNKIILMGRLTRDPELRHTQTGTAVASFSLAVDRDFKDKATGD
                     RTTDFIDVVAWRQTGEFVSRYFTKGRMAVVEGRLQIRDWTDKDGNKRRSAEVVADNVY
                     FGDSKRDAESAGPYSGGYSAPSGGRSPAPSGFGAPDMGDQFAELSDDDGELPF"
     gene            complement(4871..5167)
                     /gene="rpsF"
                     /locus_tag="ACZPAQ_01010"
     CDS             complement(4871..5167)
                     /gene="rpsF"
                     /locus_tag="ACZPAQ_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR0902583.1"
                     /translation="MAKINANYEALYILDPALGEEEITALVEKFKGVVEANGTVSEID
                     QWGKRRLAYPINDLNEGYYVLMTFNAAAAIPHELDRLFRINDSVMRSLIVCKDQ"
     gene            complement(5348..5962)
                     /gene="lexA"
                     /locus_tag="ACZPAQ_01015"
     CDS             complement(5348..5962)
                     /gene="lexA"
                     /locus_tag="ACZPAQ_01015"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR0902584.1"
                     /translation="MEKLTAKQQKIYDYIQTFSAEHGYPPSVREIGAAVGLKSPSTVH
                     FHLKGLEEAGVIIKAEGKTRAITLSGTGRPVAEEQDPHADQIPVVGSVAAGSPILAEE
                     CIEDYLTFDTQGLSGEHFALRVRGESMLDAGILPGDLVVVHRQQEVHSGEIVVALFED
                     EATVKTYRRKDGHVWLMPANDDYTPIDGDRAEILGKVVAVVRRY"
     gene            6303..6779
                     /locus_tag="ACZPAQ_01020"
     CDS             6303..6779
                     /locus_tag="ACZPAQ_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0902585.1"
                     /translation="MSSLNMDRGVLEHQMHQAHRNAVQAELSAAGLQEVGHPLLLCIL
                     RSAGERCPDGQAQRELAELLNISPPAVAASLKSLEKKGYIHREPEPGDARRNRVRLTA
                     KGEKAVDDCFACLQRVTRRMFTGFTTEEQAVMRGFYLRMLENLQAPLAGVRASPLA"
     gene            6864..8654
                     /locus_tag="ACZPAQ_01025"
     CDS             6864..8654
                     /locus_tag="ACZPAQ_01025"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0902586.1"
                     /translation="MIDLLQLFLTHLQGYKRQAVWSPILIVVEVICELLLPLVMAEIV
                     DTAIPAGDETYIFQLGAVMLVLAGVAMLCGVLAAKYSAFASQGFGANLRQCLFDKVQE
                     FSFADIDRFSSASLITRMTNDVNAMTMMLAMGLRMLVRAPVMLVAALCISFYLNARLA
                     LVLVVVIPLMVLVIGILMKVCTKLFETMQTKIDNLNNTLQENLVAIRVVKAFVREGYE
                     RIKFKKSNDELMDAALAVGLRIIAIMPVMMLALNGATVAVLYFGGGMVMGGTFELGDL
                     QAFINYIVQILMSVMMVAMSLLQLSRAQACAHRIKEVLETEPSVENKPEGEICRAALP
                     GEAADGTDHVLPAPRGEVEFRDVSFKYVASGSGDDVLSHISFHVRPGQFVAIVGGTGT
                     GKSTLVNLIPRFYDVTGGQVLLDGVDVRNYPLEELRGRIGMVLQTNILFSGTIRENLL
                     WGNPEATEEEMIQAAKDAQAYDFIMSFPDGFDTSLSQGGVNVSGGQKQRLCIARAMLR
                     KPAVLILDDSTSAVDSATEAAIRESFATNLKDTTVIIIAQRISSVQYADEILILEDDH
                     IAARGTHEELLATSPIYQEIYQSQQEGVGE"
     gene            8654..>9116
                     /locus_tag="ACZPAQ_01030"
     CDS             8654..>9116
                     /locus_tag="ACZPAQ_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR0902587.1"
                     /translation="MPGPNRAPRGGYQKPKDLGKTISRLLGYLTRSKLPLMAVFGCLL
                     ISVFTNIGGSYMMRGIINDFIWSGCTDFAGLGRAILVLVGIYLIGCAATYGQSAVMVR
                     LAQRGINRLRKDLFDKLQDLPLSYFDKHPHGELMSRFTNDADNVQLALEQSV"
BASE COUNT         1715 a         2727 c         3041 g         1633 t
ORIGIN      
        1 acccacgcac tgatccaggc ggtgaagggc aaccgggagg agggcctgtt tttcagcggg
       61 gccagcgtgg gcctgctgga ccggatgtgc tccgtccggg agctgatgga ggagctgaca
      121 agagaatgga gggcattggc atgaaattgg cctttttata tgcggggcag ggcacccagc
      181 acccgggaat ggggcgggac ctgtacgaga cttatcccgc cttccggcag gtgctggaca
      241 gcgcccagct ggactttgac ctgaaagagc tgtgcttcga gggaccggag gagaaactgt
      301 cccagaccca gtacacccag ccctgcatgg tggcctttgc cgccggggtg acggcggtgc
      361 tgcgggagaa ggggatcatc ccggaggtgg ccgccggcct gagcctgggc gagtactccg
      421 ccctccaggc cgccggggtg ttcgactcca agacggcggt ggagctggtt gccttccgag
      481 ggaaagccat ggcccaggcg gtgcaggacc ggccctgcgg catggccgcg gtgctgatgc
      541 tgggccggga gcagctggcg gagtgctgcc gtcaggccgg ggaggccggt gtggtggaga
      601 tcgccaacta caactgcccc acccagctgg tcatcgccgg ggacgccgcc gccgtggaga
      661 aggcgggtga gctggccctc caggcggggg ccaagcgggt ggtgccgctg aaggtgagcg
      721 gccccttcca cacctccctg atggcgcccg cgggggaggc tttggcggaa aagttccgca
      781 gcgtcgcctt tggggagatg agcttcccgg tgctgttcaa ctgcaagggc ggtcccatgg
      841 aggcgggaga gaccatcccg gagctgctgg tgcgccaggt tcagagcagc gtctatctgg
      901 aggacaccat ccgcgccatg gcggggatgg gcgtggacac catcgtggag atcgggccgg
      961 gcaaggctct gtccgcattt gtgaaaaaga cggaaaaggg catcaagacc tacgcggtgg
     1021 agacggtgga ggacatcgag aagctgtgtg aggcgctgaa aggagagcag gcatgagctt
     1081 gaagggaaaa gtggccctgg tcaccggcgg gagccggggg atcggccggg cggtctgtct
     1141 ggagctggcc cgccaggggg cgtacgtggc ggtgaactac gccgggaacg aggctgcggc
     1201 ccaggagacg gcccaggcgt gccgggagct gggcgtgcgg gcagtggtcc tccgggccga
     1261 tgtggctgac gctgcggcct gcgacgcaat ggtggctcag gtgctggagc agctgggccg
     1321 gctggacatc ctggtgaaca acgcaggcat cacccgggac ggcctgatgc tgcggatgcc
     1381 cgaggcagac tgggacgcgg tgctggacac caacctaaag ggggccttcc actgtatgcg
     1441 ggccgcctat aagaccatga tgcgccagaa gagcggccgg gtagtcaaca tcagcagcgt
     1501 agtgggcctg cggggcaacg ccggccagac caactactcc gccagcaagg cgggtctcat
     1561 tggtctgtcc aagtccctgg ccaaggagct ggccacccgg aacgtcaccg tcaacgtggt
     1621 ggcccctggc tttatcacca ccgatatgac cgatgccctg ccggaggggg cgcgggaggc
     1681 cctgctgtcc actatcccca tgaagcggct gggcgcggcg gaggacgtgg cccgggcggt
     1741 ggccttcttt gctggcgagg gcgccggcta tgtcacgggc caggtgctgt gcgtggacgg
     1801 aggaatggcg gtttgagccg cagaaaggaa actgctatgg aaaaaagacg agtagttatc
     1861 accggcctgg gcgcggtcac ccccctgggc cataccgccc gggaaacctg ggaggcggcc
     1921 aaagcgggtg tgtgcggtat cgcccccatc accctctatg accactccgc ccagaaggtg
     1981 tccctggccg gcgaggtgaa ggagttcgac ccctccggcg tgctggagaa gaaggagatc
     2041 cggcggatgg accgcttcgc ccagtttgcc gtgacggcgg ccgcggaggc catggcccac
     2101 agcggcctgg acctggagcg ggaggacagg gcccggatcg gcgtcgccct gtccagcggc
     2161 atcggcggca tcggcaccct ccagacctcc tgcgagactg ggcagagccg gggctttgac
     2221 cgggtctccc ccttcttcat ccccatggtg atcaccaacc tggcggccgg ccatatcgcc
     2281 atccgcttcg gactgcacgg catgtgcatc tgcccggtgg cggcctgcgc cggcggcgcc
     2341 aatgcggtgg gtgacgcttt ccgccacatc cgggacggct acgccgaggt gatggtggcc
     2401 ggcggagccg aggcctccat cacgcccctg gccatgggcg gattcacctc catgagcgcc
     2461 ctgaccgacg cctccgatcc cagccgtgcc tccatcccct ttgacaagga gcgcagcggc
     2521 ttcgtcatgg gcgagggggc cgcggtgctg atcctggagg agctggagca cgccagggcc
     2581 cgaggggccc agatcctggg cgagatcacc ggctacggcg ccacctgcga cgcctaccac
     2641 atcaccgccc ccgacccgga gggcgcctgg agcgccgcct gcatggagca ggccctggcc
     2701 gatgccggcc tggccccgga ggcggtggac tacatcaacg cccacggaac ctccaccccc
     2761 ctcaacgaca gcggggagac cgccgccatc cgccgggtgt tcggcgccca cgcggaccgg
     2821 ctgatggtca gctccactaa gtccatgacc ggccacctgc tgggagccag cggcgcggtg
     2881 gaggctgccc tgtgtgtgtt ggccctgaag gatgggtttg tccccgccac tatcaactat
     2941 caggtcccgg acccggcctg tgacctggac atcgtgccca acgagggccg ggaggcggac
     3001 atccgctgcg ccatatccaa ttctctgggc ttcggcgggc acaacgcctg cctggtgttc
     3061 cagaagtggg agggctgaga catggagctt gacgccaatc agatccagga gatcctgccc
     3121 caccgcccgc cctttctgct ggtggaccgc atcaccggcg gagagcccgg cgtctgggcc
     3181 cagggcatca aatgcgtcac tgtgaacgag cccttcttcg cgggccactt tccggggtat
     3241 cacgtgatgc ccggagtgct cattatggaa gcgctggccc aggtgggggc tgtggctatc
     3301 ctgtccctgc cggagaacaa ggggaagctg gccttcttcg gggggatcaa gaacgcccgg
     3361 ttcaagcgcc aggtggtgcc cggagacgtg ctggagctgc gctgtgagct gacccgccag
     3421 gtgggcagcg tgggcttcgg caaggcggag gcccgggtga acggcaagct ggcggcccag
     3481 ggagaattga ccttcgctgt tgcacagccg ggcgatccct gataaaatgg actccagcag
     3541 agaaaacagg ggaaaagagg aggggtccat cttgctggag aaggatttta acgaggtgta
     3601 caccaaattc aagctgcatt tttaccgggc ggtgttcggg cgtttcaagc ggcgggaggc
     3661 cacgctgacc acagtggaga ccttctgcat ggagattatc ctggccctgg gggagcccac
     3721 ggtgaacgag ttttctaatt tcgtgggcat ctctcccccc aacgcggcct acaagatcaa
     3781 cagcctggtc cagaaggggt atgtagaaaa gcggcagtcc caggaggata agcgggagta
     3841 ccacctggtg gcgacccaga agtatctgga ttattacaac atcagctatc attatctcaa
     3901 cactgtcatg agccgtatcc gggcccgctt ttccccggag gacgtggcca agctggaggg
     3961 gatgctggag atcatctccc gggagctgat gcccgagatc cccctggggg accagatcca
     4021 tcaagcagaa caggcataaa aaacccggcg atcacaggat cgccgggttt ttatccatat
     4081 aaagctcgat cagtcagtga cgtagggcag cagggcgacc tgacgggcgc gcttgatggc
     4141 ctcagtcagc tggcgctgat gcatggcgca ggtgccggtg gtgcggcggg gcagaatctt
     4201 ggcccgctcg gagatataac ggcgcagctt ggcggcgtcc ttatagtcga tgcactccac
     4261 cttgtccacg cagaaggcgc agaccttgcg gcgcttgcgg ccgcgagggc cccggttatc
     4321 acgatccata ggcatgagag aaacctcctt ttcagaccat atcggtcaaa gtatcacggg
     4381 tgtcccctct cagaagggca gctccccgtc gtcatcagaa agctcggcaa actggtcgcc
     4441 catgtcgggg gcgccgaagc cggagggggc gggagaacgc ccgccggaag gggcggaata
     4501 gcccccgctg tacgggccgg cggattccgc gtcccgcttg gagtcaccga aatagacgtt
     4561 atcggcaacc acctcggcac tgcggcgctt gttgccgtcc ttgtccgtcc agtcgcggat
     4621 ctggaggcgg ccctccacca cggccatgcg gcccttggtg aagtagcggc tgacaaactc
     4681 gccggtctgc cgccaggcaa ccacatcaat gaaatcggtg gtgcggtcgc cggtggcctt
     4741 gtccttaaag tcccggtcca ctgccagaga gaaggaggcc acagccgtcc cggtctgggt
     4801 gtgccggagc tcggggtcac gggtcagacg acccatgagg atgatcttgt tgagcatatg
     4861 cccgcctccc tcactggtcc ttgcagacga tcagggaacg catcacgctg tcgttgatcc
     4921 ggaacagacg gtccagctcg tggggaatgg cggcggcggc attgaaagtc atcaggacgt
     4981 agtagccctc gttcaggtcg ttgatgggat aagccagacg gcgcttgccc cactgatcga
     5041 tctcagagac ggtcccgttg gcctccacca cacccttgaa cttctccacc agagcggtga
     5101 tctcctcctc gcccagggcg gggtccagga tgtagagcgc ctcgtagttc gcgttgattt
     5161 ttgccatagt tcttgcacct ccttatggac atatggcccc cggaagaccc gggagcaagg
     5221 atatctcctg tctcatcaga aagacaggct taatcattat ataggaaatc cccgggatgt
     5281 caagagaaaa tcccggggat ctggcaaaaa ttttatttca gaggcaggaa aaacacctgg
     5341 gcgggactca gtagcggcgg actaccgcca ccaccttgcc caggatctcg gcccggtcgc
     5401 cgtcgatggg ggtatagtcg tcgttggccg gcatgagcca cacatggccg tccttccggc
     5461 gataggtctt gacggtggcc tcatcctcaa acagggccac cacgatctca ccgctgtgga
     5521 cctcctgctg gcggtggacc accactaagt ccccgggcag gatgccggcg tccagcatag
     5581 attccccccg cacccgcagg gcgaagtgct ccccggacag gccctgggta tcaaaggtga
     5641 ggtagtcctc aatgcactcc tccgccagga tgggggagcc ggcagccaca ctgcccacca
     5701 ccgggatctg atcggcgtgg gggtcctgct cctccgccac cggccggccg gtgccggaca
     5761 gggtgatggc ccgggtcttg ccctctgcct tgatgatgac gccagcctcc tccagcccct
     5821 tcagatggaa atggacggtg gagggggact tgagacccac agccgcgccg atctcacgga
     5881 cagaaggggg atagccatgt tccgcggaaa aggtctggat gtagtcgtag atcttctgct
     5941 gcttcgcagt gagtttctcc atggtctgac tcctttccac agatgggcgt cgtcggttca
     6001 tttaataaaa gcatagcaca ggaaagcaaa aaagtcaaac gaatgttcca aataagcttt
     6061 ctgaaaatga aataaatgga ggatggggag cggtggggcc tgtatcccgt gcagggccga
     6121 tgggggcact acgccctaca cggggacaca gcctccgcag aggggagggc cggtccattt
     6181 atgaacaatt tttgaaacat acagggctcc ccttgacttt ttatggccga atgctaaaat
     6241 aatcagcatg ttaattatta agccgttaat gattgaaaaa tattccgtaa gggggcggag
     6301 atatgtccag tctgaacatg gatcggggtg tgttggaaca tcagatgcat caggcgcatc
     6361 ggaatgcggt gcaggcggaa ttgagcgcgg cgggactgca ggaagtgggc catccccttc
     6421 tgctgtgcat cctgcgcagc gcgggggagc gctgcccgga cgggcaggcc cagagggagc
     6481 tggcggagct gctgaacatt tcgccgcccg cagtggcagc atccctgaaa tccctggaga
     6541 aaaaggggta tattcaccgg gagccggagc ctggggacgc ccggaggaac cgggtgcggc
     6601 tgaccgccaa gggggagaag gcggtggacg actgcttcgc ctgcctgcag cgggtgaccc
     6661 gcagaatgtt caccggcttt accaccgagg agcaggcagt gatgcggggc ttctatctgc
     6721 ggatgctgga gaacctccag gctcccctgg ccggcgtgcg ggcttctccc cttgcctgag
     6781 ggcgggaacg atgaagcggc gggcgcgcgg ctggatttct cgtcattttg accataagct
     6841 tcttaaaaaa ccaagaaagg aagatgatcg atttgcttca gttgtttctg acccacctgc
     6901 agggctacaa gcggcaggcg gtctggtctc ccatcctcat cgtggtggag gtcatctgtg
     6961 agctgctgct gcccctggtc atggcagaga tcgtggatac ggccatccca gccggcgacg
     7021 agacttacat ttttcagctg ggtgcggtga tgctggtgct ggcgggcgtg gccatgctgt
     7081 gcggtgtgct ggcggccaag tattctgcct ttgccagcca gggctttggc gccaatctgc
     7141 gccagtgcct cttcgacaag gtgcaggagt tctcctttgc ggacattgac cgtttctcct
     7201 ccgcctccct catcacccgt atgaccaacg atgtcaatgc catgaccatg atgctggcca
     7261 tgggtctgcg gatgctggta cgggctccag tgatgctggt ggccgccctg tgtatcagct
     7321 tctatctgaa cgccaggctg gcactggtgc tggtggtggt cattcctctg atggtgctgg
     7381 tgatcggcat tctgatgaag gtgtgcacca agctctttga gaccatgcag accaagatcg
     7441 acaacctgaa caatacgctc caggagaatc tggtggccat ccgagtggtg aaggcctttg
     7501 tccgggaggg ctacgagcgc atcaagttca agaagtccaa cgatgagctg atggacgccg
     7561 ctctggcggt gggcctgcgg atcattgcga tcatgccggt gatgatgctg gccctcaacg
     7621 gggccacagt ggcggtgctc tacttcggcg gaggcatggt catggggggc acctttgaac
     7681 tgggcgacct ccaggccttc atcaactaca tcgtgcagat cctcatgagc gtgatgatgg
     7741 tggccatgtc cctgctccag ctctcccggg ctcaggcctg cgcccaccgc atcaaggagg
     7801 tgctggagac agagccctct gtggagaaca agccggaggg ggagatctgc cgggcggccc
     7861 tccccgggga ggcggcggac ggaacggacc acgtcctgcc cgcccccagg ggcgaggtgg
     7921 agttccggga cgtctccttc aaatatgtgg cctcgggcag cggggacgac gtgctctccc
     7981 acatcagctt ccatgtaagg ccggggcagt tcgtggccat cgtgggcggg accggcacgg
     8041 gcaaatccac tctggtcaac ctgatccccc ggttctacga tgtcaccggc ggccaggtgc
     8101 tgctggacgg ggtggatgtg cggaactacc ccctggagga gctccggggc cgcatcggca
     8161 tggtgctcca gaccaacatc ctcttttccg gcaccatccg ggagaatctg ctgtggggca
     8221 acccggaggc cacagaggag gagatgatcc aggcggccaa ggacgcccag gcctatgact
     8281 tcatcatgtc gttccccgac ggcttcgaca ccagcctgtc ccagggcggc gtcaacgtgt
     8341 ccggcggcca gaagcagcga ctgtgcatcg cccgggccat gctccgcaag ccggcggtgc
     8401 ttatcctgga cgactccacc tctgccgtgg actcagccac agaggcggcc attcgggagt
     8461 ccttcgccac caacctgaag gacaccactg tcatcatcat tgcccagcgg atctcctcgg
     8521 tgcagtacgc cgatgagatc ctcattctgg aggatgacca cattgcggcc cggggcaccc
     8581 atgaggagct gctggctacc agtcccatct atcaggagat ctatcagtct cagcaggaag
     8641 gagttggtga ataatgccgg gacccaatcg cgccccccgg gggggctatc agaagcccaa
     8701 ggatctgggc aagaccatat ccaggctgct gggttatctc acccgcagca agctgcccct
     8761 gatggccgtg ttcggatgcc tgctgatctc ggtgttcacc aacatcggtg gctcctacat
     8821 gatgcggggc atcatcaacg acttcatctg gtcgggctgc accgacttcg ccggcctggg
     8881 ccgggccatc ctggtcctgg taggaatcta cctcatcggc tgcgcggcca cctatggaca
     8941 gtccgccgtc atggtgcggc tggcccagcg ggggatcaac cggctgcgca aggacctgtt
     9001 cgacaaattg caggatctgc cgctgtccta ctttgacaag cacccccacg gcgagctgat
     9061 gagccgcttc accaacgacg cggacaacgt gcagctggcc ctggagcaga gcgtgg
//
LOCUS       DBMDSJ010000019         8893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2480_length_8893_cov_1.974067, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000019 DBMDSJ010000000
VERSION     DBMDSJ010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2480_length_8893_cov_1.974067"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="ACZPAQ_01035"
     CDS             <1..863
                     /locus_tag="ACZPAQ_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HIR0902588.1"
                     /translation="MQSGCPQELESVHRPHTVRQVDEAVAAARKAKLNNVSLDLIYGL
                     PGQTMDSWKATVEHALSLIPQHLSCYGLKVEEGTPLARRAAEGEILPDDDQQADLYLW
                     TVGRLERAGYPQYEISNFAKPGYESRHNLRYWLTRPYIGFGPGAHSDFGGRRYSFVRD
                     LDRYIDGVLKGGEIIDSEELIPQRERGGEYLMLRLRTAQGIEEWEYRSAYFMDFAPLE
                     TRLRQFQAQGWAEQAGGRWRLTPKGFLVSNQLIGDLLERQEESSLDQLLPRARARFSG
                     SQDGPFVDSV"
     gene            1061..2356
                     /locus_tag="ACZPAQ_01040"
     CDS             1061..2356
                     /locus_tag="ACZPAQ_01040"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR0902589.1"
                     /translation="MTKYLIEGGHPLHGTIEISGAKNAAVAIIPAALLVDGVCRIENV
                     PQISDVTLILQILRELGADVRTINRTTVEIDCSHIRNRQVPYELARKIRASYYLVGAL
                     LGRFGWAEVPLPGGCNLGGRPIDQHIKGFVAMGAEVDVRNGLIYAQVPGRGCLAGGQV
                     YLDIVSVGATMNIMLAAALAEGMTVIENAAKEPHIVDLANFLNSMGADIMGAGTDVIK
                     VRGVQRLRGGSYSIIPDQIEAGTYMAAVAAAGGELLVKNVIPKHLDCISAKLVEMGVD
                     VEEQDDAVLVRRSGPITRTNVKTMPYPGFPTDMQPQIAAVLCLAQGTSVLTEGVWDNR
                     YRYVDEFRRMGAKIQVDGKVAVIEGVPALTGAPVHACDLRAGAAMVIAGLAAQGTTEI
                     DGVYHIERGYETLVEKLSGVGAKIQVLFLPDDEAQTQAG"
     gene            2444..3280
                     /locus_tag="ACZPAQ_01045"
     CDS             2444..3280
                     /locus_tag="ACZPAQ_01045"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0902590.1"
                     /translation="MEVCTLASGSSGNSLLICCGSTYVLLDAGISARRITSALKALGA
                     DPTRLSAVLVTHEHTDHVSGLATLTKQLGVPVYATAPTLDRLERKVPALARLGRALEA
                     GTGFQLGELWVESFSTPHDAAGSVGYAVTGGGARMALATDLGHLTPQVWDAARGADLL
                     VCETNHDEDWVRSGPYPYALKQRILGDFGHLSNEAGAELAGRAALAGTRTILLAHLSS
                     ENNTPARAREVVSRRLSALGVRPEEDITLAVAPRSECGALYQLERGGALRAFPAREAS
                     LC"
     gene            3274..3756
                     /locus_tag="ACZPAQ_01050"
     CDS             3274..3756
                     /locus_tag="ACZPAQ_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR0902591.1"
                     /translation="MLSVYLICVGKLKEKFYQEACAEYIKRLSPYCRLTLVELPEEKL
                     PQDPSQAQIDAALEKESQAIRAKLPPSTSLVCLCVEGRLRSSEELSALVGTWEHSSAK
                     HLAFVIGGSYGLAERLKGESWVKLSMSPMTFPHHLARVMLLEQLYRAFKIREGSSYHK
                     "
     gene            3888..5615
                     /locus_tag="ACZPAQ_01055"
     CDS             3888..5615
                     /locus_tag="ACZPAQ_01055"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR0902592.1"
                     /translation="MSYLDAYHAWLNSPALSAAEKAELASIQGDDKEIESRFFDQLSF
                     GTAGLRGTMGVGLYRMNVHVIRHATQAFAQVILEEGPQAAARGVAVCFDCRNNSDVFA
                     REAACVMAGNGIPVRLFESLRPTPELSFAVREYGCIAGINVTASHNPKEYNGYKVYWQ
                     DGAQLPPKHADEVARKMKELDVFDCVKTMDYDQAVAQGRITLMGAETDEKFLANVMEQ
                     VNDKAVVEQMADTFTMVYTPFHGTGYKLIPEALKRLGMKHVICVPQQMVIDGDFPTVV
                     SPNPENPEGFYLAVELAKEHGADFILGSDPDADRVGIMVRTKEDDFVVISGNQTGVLL
                     LDYLIGAKRRTGKMPDKPVALKTIVTTEMARKVAEVNGLQCYDTFTGFKFLAEKKDEL
                     ESSGAGKVIFSYEESYGYMLGDYVRDKDAVSASVELTEMAAWYASQGMTLYDALQALF
                     KKYGYYGERTMNLVMPGLDGLKKMADLMAGLREQPPAEIAGVAVKQQKDYKDGSVVET
                     ATGARSQMELTGSNVLRYEMADGTSLIVRPSGTEPKVKVYILANGADKEECDGKVAKY
                     AAWAESLKD"
     gene            5947..6168
                     /locus_tag="ACZPAQ_01060"
     CDS             5947..6168
                     /locus_tag="ACZPAQ_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902593.1"
                     /translation="MASGQTQYYGLNQWEAADQVLRTEFNADNQKIDGALKAMRDLIV
                     SPSVLNAVLDKLRSDINALSARVDKLEGK"
     gene            complement(6234..>8893)
                     /locus_tag="ACZPAQ_01065"
     CDS             complement(6234..>8893)
                     /locus_tag="ACZPAQ_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902594.1"
                     /translation="KVRMLDKDEIITVPNAPTYTYTSGQDIDEVYKDLGKSVCTLRDN
                     AKDEGYTWTAYVNGKEDKDVDDNIPTSKDDSTWKYTGKGTVTEIYIDDADATVTVVEI
                     NYYLGQVSKVKSDSKGEYVTVKAISTEPKLDDNDFYAEGYEEDDYVVFTVDYNEDEDF
                     YICELMAPETVNGEVTRVEKDKDAETGKGETGETYLKLADGAKYSYSGDGHIVYDLDD
                     DNVKAHPALNEQYTLYLDPNGYVLGFVQETGSTQYLYVKDSDEELRDWVAKVVLADAT
                     SVKADLDDEYKDGKDKVKIDWVDKDQDKKDKTNIDEKVWAYTVGEDKIYTLKAVDSKT
                     MTNAEINNGKAYISDGKNDFIVDKKTVFVDVEGETAYTGYNEVPDVDNATLAYVLSTK
                     NGNVAEIVFIVDGDVYDSASTYFMLSKTTRESLKYDGDYYWEYTNAYVNGVKQSVYVD
                     QDLGALEVGHLYKATKTVDEKYITGIEDVELKDNTVNARGDGAFWLTTVEDAKVKYDV
                     DDDTVFVYVDVEPKNGENLSKGYDYTITDGDVKDMYVGTEEETYDGVKFDYMTYVTVV
                     KDSNDHNTADLVYIIHKPVVEMVKVTLALTNATVTVNGVEYTENGSISVKKGTELNWT
                     AKAKEGYNLTTPASGKVTANSDTTITATASADTLSVKVEGHAAANPTSITANQAADIT
                     VTAEKGYTITDVTCAQAAKIKSNGDGTWTVSVPAGTEDIAIKVDTEAVSYTLNFAASG
                     VWDFEVKDAKGQTVNDGDEVIVGETYTFKATEKTASDTHNGAREFRVDGAAVCDAKLT
                     AEGKNAVVKSVKVTASEADFKLAQKLAVVDGKLYTEEACQNEQSAAPNQETTLYYIES
                     AAVKAVLEGEYTVVADDDGVLTLDIGQKR"
BASE COUNT         1606 a         2753 c         2787 g         1747 t
ORIGIN      
        1 ggatgcagtc cggctgcccc caggagctgg agtccgtcca ccgcccccac accgtccgac
       61 aggtggacga ggcggtggcg gccgcccgga aggccaagct gaacaacgtc tctctggatc
      121 tgatctacgg cctgcccggc cagaccatgg acagctggaa agctacggtg gagcacgccc
      181 tgtccctgat cccccagcac ctgtcctgct atggactgaa ggtggaggag ggcacccctc
      241 tggcccgccg ggcggcggag ggggagatcc tgcccgacga cgaccagcag gcagacctgt
      301 acctctggac cgtgggccgg ctggagcggg ccggctaccc ccagtatgag atctccaact
      361 tcgccaagcc gggctatgag tcccggcaca atctgcgcta ctggctcacc cggccctata
      421 tcggcttcgg ccccggcgcc cattccgact tcggcgggcg gcggtactcc tttgtccggg
      481 acctggaccg gtacatcgac ggcgtgctca agggcgggga gatcatcgac agcgaggagc
      541 tcatccccca gcgggagcgg gggggcgagt acctgatgct ccggctgcgt accgcccagg
      601 gaatcgagga gtgggagtac cgcagcgcct atttcatgga ctttgccccc ctggagaccc
      661 ggctccgcca gttccaggcc cagggctggg cggagcaggc cggcggccgg tggcggctga
      721 cgcccaaggg ctttctggtg tccaatcagc tcatcggcga cctgctggag cggcaggagg
      781 agtccagcct ggaccagctg ctcccccggg ccagggcccg cttttccggc agccaggatg
      841 gtccctttgt ggattctgtt taagaatcgg gaaaatgttt ccggcctttg gaccgatttt
      901 acacactttt cacgcaaatg taattttatt tgccttttgg atataatagt gttacaatat
      961 agttgtatca gaccgcccac aggcgggacc tgtttggact acttatggca ttttgccgta
     1021 tagacataag gacctcatta cgtttgaaga gggtgcagct ttgaccaaat atttgattga
     1081 gggcggacat ccgctccatg gaaccattga gatcagcgga gccaagaacg ccgctgtcgc
     1141 catcatcccc gccgccctgc tggtggacgg ggtctgccgc attgaaaatg ttccccagat
     1201 cagcgatgtg accctgatcc tccagatcct ccgggaactg ggggcggacg tgcgcaccat
     1261 caaccgcacc acggtggaga tcgactgttc ccacattcgg aaccgtcagg tcccctatga
     1321 gctggcccgg aagatccgag ccagctatta tctggtgggg gccctgctgg gccgcttcgg
     1381 ctgggcagag gtgcccctgc ccggcgggtg caatctgggc ggacggccca tcgaccagca
     1441 catcaagggc tttgtggcca tgggggccga ggtggatgta cgcaacggcc tgatctatgc
     1501 ccaggtcccc ggtcggggct gcctggccgg cgggcaggtc tatctggaca tcgtctccgt
     1561 gggcgccacc atgaacatca tgctggccgc cgccctggcg gagggcatga ccgttatcga
     1621 gaacgccgcc aaggagcccc atatcgtgga cctggccaac ttcctcaact ccatgggggc
     1681 cgacatcatg ggcgccggca ccgacgtcat caaggtgcgg ggcgtccagc gcctgcgggg
     1741 cggctcctac tccatcatcc ccgaccagat cgaggcgggc acctatatgg ccgcggtggc
     1801 tgccgccggg ggcgagctgc tggtgaagaa cgtcatcccc aagcatctgg actgcatcag
     1861 cgccaagctg gtggagatgg gggtggatgt ggaggagcag gacgacgcgg tgctggtccg
     1921 ccgctccggc cccatcaccc gcaccaatgt gaagaccatg ccctatcccg gctttcccac
     1981 tgacatgcag ccccagatcg ccgcggtcct gtgcctggcc caggggacca gcgtgctcac
     2041 cgagggcgtg tgggacaacc gctaccgcta tgtggacgag ttccgccgca tgggggccaa
     2101 gatccaggtg gacggcaagg tggccgtcat cgagggggtc cccgccctga ccggcgcgcc
     2161 ggtccacgcc tgtgacctgc gggccggggc agccatggtg atcgccggtc tggctgccca
     2221 gggcaccacg gagatcgacg gggtgtacca catcgagcgg ggttacgaga ccctggtgga
     2281 gaagctgtcc ggcgtggggg ccaagatcca ggtgctcttc ctgcccgacg acgaggccca
     2341 gacccaggcg ggctgacctt tgcacatgct ccgggcggcg gcgatctgcc gccgcccgtt
     2401 ttcgcgccct ggggggcgcc actctttctc cgggaggcgg cacatggaag tgtgtactct
     2461 ggccagcggc tccagcggga attccctgct gatctgctgc ggctctactt atgttttgct
     2521 ggacgcgggc atctccgccc gccggatcac ctctgccctg aaggcgctgg gggccgatcc
     2581 cacccggctc agcgcagttc tggtcaccca cgagcacacc gaccacgtct ccggtctggc
     2641 caccctgacc aagcagctgg gggtgcccgt ttacgccacc gcccccactc tggaccgcct
     2701 ggagcgaaaa gtcccagcac tggcgcggct gggccgggcg ctggaggccg gcactggctt
     2761 ccagctgggg gaattgtggg tggagtcctt ctccaccccc cacgacgccg cgggcagtgt
     2821 aggttacgcc gtcaccggcg gcggggcgcg gatggcactg gccaccgatc tgggccacct
     2881 gacgccccag gtgtgggatg cggcgcgggg ggcggaccta ctggtgtgtg agaccaatca
     2941 cgacgaggac tgggtccgct ccggccccta tccctacgcc ctgaagcagc gcatcctggg
     3001 ggacttcggc cacctgtcca atgaggcggg ggccgagctg gccggccggg ctgccctggc
     3061 cgggacccgg accatcctgc tggcccacct gtcctccgag aacaataccc cagcccgggc
     3121 ccgagaggtg gtctcccgcc gcctgtccgc cctgggggtg cggccggagg aggacatcac
     3181 cctggcggta gccccccggt cggagtgcgg ggcgctgtac cagctggagc ggggcggagc
     3241 tctccgggcc tttccggcaa gggaggcatc cctgtgctga gcgtctatct gatctgtgtg
     3301 ggcaagctga aggagaaatt ctatcaggag gcctgtgccg aatacatcaa gcgcctgtcc
     3361 ccctactgca ggctcaccct ggtggagctg cctgaggaga agctgcccca ggacccctct
     3421 caggcgcaga tcgacgccgc cctggagaag gagagccagg ccatccgggc caagctgccc
     3481 cccagcacca gcctggtgtg cctgtgcgtg gagggcaggc tgcgctccag cgaggagctg
     3541 tccgctttgg tggggacctg ggagcacagc tccgccaagc acctggcctt tgtcatcggc
     3601 ggctcctacg gcctggcgga gcgcctgaag ggggagtcct gggtcaaact gtccatgtcc
     3661 cccatgacct tcccccacca cctggcccgg gtcatgctgc tggaacagct ctaccgggcc
     3721 ttcaagatcc gggagggctc cagctaccac aaatgacagg gtcccgggcc tgaaaacatg
     3781 ctcaccgtgg atccgtccaa aaatccgctg gaaattcatg cctgcctgtg gtacaatgtc
     3841 cacaacagca tgtcccagcg gattttattg attggaggtc ctacgatatg tcctatcttg
     3901 atgcttatca tgcatggctg aacagcccgg ccctctcggc cgccgagaag gcagagctgg
     3961 catccatcca gggcgatgac aaggagatcg aatcccgctt ttttgatcag ctttccttcg
     4021 gcaccgcggg cctgcggggc accatggggg tggggctgta ccggatgaat gtccatgtca
     4081 tccgccacgc cacccaggcc tttgcccagg tcatcctgga ggaggggccc caggccgccg
     4141 ccaggggggt ggccgtctgc ttcgactgcc gcaacaactc cgacgtcttt gcccgggagg
     4201 ccgcctgtgt catggcgggc aacggcatcc ccgtgcgcct gttcgagtcc ctgcgtccca
     4261 ccccggagct gtccttcgcg gtgcgggagt acggctgcat cgccggtatc aacgtcaccg
     4321 ccagtcacaa ccccaaggag tacaacggct acaaggtcta ctggcaggac ggtgcccagc
     4381 tgccccccaa gcacgccgac gaggtggccc ggaagatgaa ggagctggac gtgttcgact
     4441 gcgtcaagac catggactat gaccaggccg tggcccaggg caggattacg ctgatggggg
     4501 cggagacgga cgagaagttc ctggccaacg tgatggagca ggtgaacgac aaggcggtgg
     4561 tggagcagat ggcggacacc ttcaccatgg tgtacacccc cttccacggc accggctata
     4621 agctgatccc cgaggccctc aagcgcctgg gcatgaagca cgtgatctgc gtgccccagc
     4681 agatggtcat cgacggcgat ttccccactg tggtctcccc caacccggag aacccggagg
     4741 ggttctatct ggcggtggag ctggccaagg agcacggcgc ggacttcatc ctgggctctg
     4801 acccggacgc cgaccgggtg ggcatcatgg tgcgtaccaa ggaggacgac ttcgtggtca
     4861 tctccggcaa ccagaccggc gtgctgctgc tggactacct gatcggggcc aagcgccgca
     4921 ccggcaagat gcccgacaag cccgtggccc tcaagaccat cgtcactacc gagatggccc
     4981 gcaaggtggc cgaggtcaac ggcctgcagt gctatgacac cttcaccggc ttcaagttcc
     5041 tggcggagaa gaaggacgaa ctggagagct ccggtgcggg gaaggtcatc ttctcttatg
     5101 aggagtccta cggctacatg ctgggggact atgtccggga caaggacgcc gtctccgcct
     5161 cggtggagct gaccgagatg gcggcctggt atgcctccca gggcatgacc ctgtatgacg
     5221 ccctccaggc cctgttcaag aaatacggct actatggcga gcgcaccatg aacctggtca
     5281 tgcccggcct ggacgggctg aagaagatgg ctgacctgat ggccggcctg cgggagcagc
     5341 ccccggcgga gatcgccggc gtggcggtaa agcagcagaa ggactacaag gacggcagcg
     5401 tggtggagac agcgaccggc gccaggtctc agatggagct caccggttcc aacgtcctgc
     5461 gctatgagat ggccgacggc accagcctga tcgtccgtcc ctccggcacc gagcccaagg
     5521 tcaaggtcta tatcctggcc aacggcgccg acaaggagga gtgcgacgga aaggtggcca
     5581 agtacgccgc ctgggccgag agcctcaaag actgaaaaga caggccggac accgggcccg
     5641 ctcctttgga gcgggcccgg tcagcttgtg gaaagagccg tcccgcaagg agttcctgca
     5701 cccgcaggcg gcggaataaa attggatgac atcatttccg gggacgtgta tctgggaagt
     5761 ggcacctctc acgcaggata ggccgcctcc atgatccctg gacatcctgc acaggcgggg
     5821 cagaaatggg catagttctg cttcacttcc ataatttgta ttgaaacgtc tcacaaaaag
     5881 ctctttccca ccggttttga gcgggtccgg atctgccggg cccagtcagg acaggaggga
     5941 ttttttatgg caagcggaca gacgcagtat tatggactga atcaatggga ggctgcggac
     6001 caggtgctcc ggacggagtt taacgcggat aatcaaaaga tcgacggggc gctgaaagcc
     6061 atgcgggatc tgatcgtcag tcccagcgtt ctcaatgcgg tactggataa gctgcgcagt
     6121 gatatcaacg ccctgtctgc ccgggtggat aagctggagg ggaagtgaga gagccccccg
     6181 gagcaaatgc tccggggggc tgttaaattg cgtgaatcag aatctcactc taatcagcgc
     6241 ttctgaccga tatcaagcgt cagcacgccg tcgtcgtcag ccaccacagt gtactcgccc
     6301 tcgagcacag ccttgacggc agcgctctca atgtagtaca gagtggtctc ctggttgggg
     6361 gcagccgact gctcattctg gcaggcttcc tcggtgtaca gcttgccatc gacaacagcc
     6421 agcttctggg ccagcttgaa atcagcctcg gaagcagtga ccttgacaga cttcacgacg
     6481 gcatttttgc cctcggcagt cagcttggca tcacagacag cagctccatc gacacggaac
     6541 tcgcgggcgc cattgtgagt atcgctagca gtcttctcgg tggccttgaa ggtgtaggtc
     6601 tcaccgacga tcacctcgtc gccatcattc acggtctggc ccttggcgtc cttgacttca
     6661 aaatcccaaa caccggaagc ggcaaagttc agggtatagc tcacagcctc agtgtccact
     6721 ttgatggcaa tatcctcggt gccagcggga acgctgacag tccaggtgcc gtcgccgttg
     6781 ctcttgatct tggcagcctg agcgcaagtc acatcggtga tggtgtaacc cttctcagca
     6841 gtcacggtga tgtcagcggc ctggttcgca gtaatgcttg tggggttggc agcagcatga
     6901 ccctcgacct tgacgctgag ggtgtcagcg ctagcggtag cggtgatggt ggtatcagaa
     6961 ttggcagtca ccttgccgga agcaggggta gtcaggttgt agccctcctt ggccttagcg
     7021 gtccagttga gctcagtgcc cttcttaacg ctgatgctgc cgttctcagt gtactccacg
     7081 ccgttgacgg tcacggtggc gttggtcagc gcgagggtga ccttcaccat ctccaccacg
     7141 ggcttgtgga taatgtagac gagatccgcg gtgttgtggt cgttgctgtc cttcacgacg
     7201 gtgacatagg tcatgtagtc gaacttcacg ccgtcgtagg tctcctcttc ggtgcccaca
     7261 tacatgtcct ttacatcgcc gtcggtaatg gtgtagtcgt agcccttgga cagattctca
     7321 ccgttcttgg gctccacatc cacatagaca aaaacagtgt cgtcgtccac gtcatacttc
     7381 accttggcat cctccacggt ggtcagccag aaagcgccgt cgccgcgggc gttgacggtg
     7441 ttgtccttca gctccacatc ctcaatgccg gtgatgtact tctcatccac ggtcttggtg
     7501 gccttgtaca gatggccgac ctccagggcg cccagatcct ggtccacgta cacggactgc
     7561 ttcacgccgt tgacataggc gttggtgtac tcccagtagt agtcgccgtc gtacttcagg
     7621 gactcgcggg tggtcttgct gagcatgaag taggtgctgg cgctgtcata aacatcgccg
     7681 tccacgatga agacgatctc agccacattg ccgttcttgg tgctcagcac ataggccagg
     7741 gtggcattgt ccacatcggg cacctcattg tagccggtat aggcggtctc gccctccacg
     7801 tccacaaaca cggtcttctt gtccacgatg aagtcgttct tgccgtcgga gatataggcc
     7861 ttgccgttgt tgatctcggc gttggtcatg gtcttggaat ccacagcctt cagggtgtag
     7921 atcttgtcct cacccacggt gtaggcccag accttctcgt cgatgttggt cttatccttc
     7981 ttgtcctggt ccttatctac ccagtcgatc ttgaccttgt ccttgccgtc cttgtactca
     8041 tcgtccaggt cggccttgac agaggtggcg tcagccagaa cgaccttggc gacccagtca
     8101 cgcagctcct cgtcggagtc cttcacgtac aggtactggg tgctgccggt ctcctgcacg
     8161 aagcccagca catagccgtt gggatccaga tacagggtgt actgctcgtt cagagcgggg
     8221 tgggctttca cgttgtcgtc gtccagatca tagacgatgt ggccgtcgcc ggagtagctg
     8281 tacttggcac catcggccag cttcagatag gtctcgccgg tctcgccctt gccggtctcg
     8341 gcgtccttat ccttttccac gcgggtgacc tcgccgttga cggtctcggg agccatcagc
     8401 tcgcagatgt agaagtcctc gtcctcgttg tagtccacgg tgaagacaac gtagtcgtcc
     8461 tcctcatagc cctcagcata gaagtcgttg tcgtccagct tgggctcggt ggagatggcc
     8521 ttcacggtga catactcgcc cttggaatcg ctcttcacct tggagacctg gcccagatag
     8581 taattgatct cgaccacggt cacagtggcg tcggcgtcgt cgatgtagat ctcggtgacg
     8641 gtgcccttgc cggtgtactt ccaggtggag tcgtccttgg aggtggggat gttgtcatcc
     8701 acgtccttat cttccttgcc gttgacataa gcagtccagg tgtagccctc gtccttggca
     8761 ttgtcgcgca gggtgcacac agacttgccc aggtccttgt agacctcgtc gatgtcctgg
     8821 ccggaagtat aggtgtaggt gggggcgttg ggaacagtaa tgatctcgtc cttgtccagc
     8881 atgcggacct tgg
//
LOCUS       DBMDSJ010000020         8753 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2529_length_8753_cov_1.955617, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000020 DBMDSJ010000000
VERSION     DBMDSJ010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_8753_cov_1.955617"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="ACZPAQ_01070"
     CDS             complement(<1..579)
                     /locus_tag="ACZPAQ_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR0902595.1"
                     /translation="MKKVDRWAALTAALLLTVSLASASQAAEAGEKIVILHTNDVHCA
                     LDDAIGYAGLAAYAGALEEQYGADRVTLVDAGDAIQGQAVGTLSQGEYIISLMNAAGY
                     DLAVPGNHEFDYGVDRLMELAAGAEFPYLSCNFTGLAAGEPVFQPYALLDYGDTTVGY
                     VGICTPESLTKTDPTYFRDEAGEAVYGFRQGGD"
     gene            complement(576..2798)
                     /locus_tag="ACZPAQ_01075"
     CDS             complement(576..2798)
                     /locus_tag="ACZPAQ_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0902596.1"
                     /translation="MNRISLVGRLRTQKLLGRTVLILLAFSLLTVGGSYLFVRGEMVS
                     RYREQGMEGTMSMLQRTSTSLDRTIQALELQIQTFWKDDDVSSLLVAPEKPDFSRTSG
                     VAAQIQGLREGNELIERCWLYLYQSGQILTDRQLLLEAGDPERPGQLERWSAAAESGT
                     PSLWCEDGKIYLLVAFPQPKKLGLLIVQLSARDLFEQFQEGWSGLSGDPVYVYDGAGE
                     PVFPNRMTYPEREELMVTEESPREGVYALSGQRDTVVCVARSQTAGWYCLTYWNSGDW
                     LPSPQEILTAFLPAAAVLLLLILILSLVILQVIYRPIQRIVESMLTGQETEAGQDEAG
                     LISAALARGQRENQRLTGLLSDVRPALAQRFFQSLLEGGQTEPEEVARMLAALESPFP
                     LEGTYLVLAVEAVPERSASAVEGEIYTRSLWRSLKRFWEGRCLAQIQPAPGGGLSAVL
                     CASPPVPGGFAIPLEDCRQALERETRDFPFSAAVGASQARGSLLELSAAWQEARQDLN
                     YRKYCCGEAGQPGPGPVGAGVYDAQVREMLQKALSGELEGARAQLADLRQAVGRAQSP
                     VQMRSMLRGCVLTQVLKVQRELGERYSAACAADSPDFWQEQERLLAELDEWGHNSQRG
                     YMDRARDYIAAHFSDSGLSLNEVSGHVGLSVSYLSSLFSKYQPPGFSHYLRQYRVEQA
                     RLLLESTDASVTEIGYQTGFNSSNSFIRTFKSLVGETPGRYRERCQGGKYENGKDDDT
                     "
     gene            complement(2831..3604)
                     /locus_tag="ACZPAQ_01080"
     CDS             complement(2831..3604)
                     /locus_tag="ACZPAQ_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR0902597.1"
                     /translation="MEWDELIRIWIIVCPLVFLGGLIDAVAGGGGLITLPAYLLAGLP
                     PHLASGTNKCGNFFGTLIATGRFLKRGDVHLPSALAGGAGALVGAWLGARLNMIMPEQ
                     ALYYLMLAVVPVMAVFLLFKRDFGTEDRSGELGVGKLVPLSLAIGLVIGCYDGFFGPG
                     AGTFLTLAFTGLCRFDLLTAAGNTKVANATSNLASLVTFALGGKVLWAVGIPAALFGI
                     VGGYVGAGLALKGGAKVIRPMFFVVLTLLVVRLVWDLLA"
     gene            3801..5288
                     /locus_tag="ACZPAQ_01085"
     CDS             3801..5288
                     /locus_tag="ACZPAQ_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HIR0902598.1"
                     /translation="MAREGFDNQKYLTMQSQHIRDRIDQFGGKLYLEFGGKLFDDFHA
                     SRVLPGFQPDSKIRMLEQLKDKAEIIIVINAGDIEKNKVRGDLGITYDSDVLRLIDAF
                     RSKGLYVGSVVVTQYTGQAAADSFRLRLEKLGVRVYRHYSIQGYPHNIAHIVSDEGYG
                     KNDYIETSRPLVVVTAPGPGSGKMATCLSQLYHHYRRGVTAGYAKFETFPIWNLPLKH
                     PVNLAYEAATADLDDVNMIDPFHLQAYGETTVNYNRDVEVFPVLAATFEKITGSCPYQ
                     SPTDMGVNMAGNCITDDGACREAACQEIVRRYYDVLSDRRQGRGGDDPVYKLELLMQQ
                     AGITPAIRPVVDQANRLEEETGEPAMAIQLPDGRMVTGKTSELMGCSAAALLNALKSL
                     AGLGGHGVHLIAQSAIQPIQTVKVQYLGSNNPRLHSDEVLIALAASANLDPKAARALE
                     ALAHLQGCQAHCSVILSPADEGTYRKLGIQLTCEPKYQTNKLYHR"
     gene            complement(5418..6833)
                     /locus_tag="ACZPAQ_01090"
     CDS             complement(5418..6833)
                     /locus_tag="ACZPAQ_01090"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR0902599.1"
                     /translation="MKQEYHRALEQICLSEEARARIISVLEQKERGLKRPRRRIGRLI
                     LVCAVLILLLLGTGAAVVSRVGVLELFFQGDTSQLEPFVQTGASAQDKNYRLTLDSSI
                     CDGDTLYVVITVEGLHEQAVENLKNNQVVADCYRAAWGREMPENLMEQGQGDISVFQF
                     QMEAGNHMSSFGFEELPAPSETSRSWQLQLTLTGLEHPQTEPIGLRLGFMEESCVLQI
                     PVDQMPQVVRVSIARELSVDWNGGEEVFVDHVEIGPTRFTYVGKYPDTASSLAPLVFF
                     RMKDGQILTRKQMGLELEMRDIPGPANVFGKELAEIVYTSRSVMDLTEIASVILGTAE
                     FSLNGEPLGEVQMKEQLYPFSIEVGEGGAEYAGPYAASMEELCKKLGAEYQWDEINKR
                     ATGVYQGISLSLTAGESLALVNGERKEIETRVRGKDGVFTQVPMPAVEYHGGISAAIL
                     FFGEVWGLEFQVLDGEIIVVP"
     gene            complement(6830..7288)
                     /locus_tag="ACZPAQ_01095"
     CDS             complement(6830..7288)
                     /locus_tag="ACZPAQ_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0902600.1"
                     /translation="MDTRELFERYGDAVFRLAWSCTGSRQDAEDVTQTVFLKVLERRP
                     DLEPGKERAWLFRVAVNECRSLWRRLKLRRTEPLEEALAVAAPQEEPVLRQVMELKAG
                     EREILYLIYYEGFSTRETASLLGISQTAVTTRLQRARKKLRERLEEEGRP"
     gene            7626..>8753
                     /locus_tag="ACZPAQ_01100"
     CDS             7626..>8753
                     /locus_tag="ACZPAQ_01100"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR0902601.1"
                     /translation="MAVKYIFVTGGVVSGLGKGITAASLGRLLKQRGLRVKVQKLDPY
                     LNVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLTVHSSVSSGKVYWNVLNR
                     ERTGDYLGATVQIIPHITNEIKRNIYSLDTPDTDVAIVEIGGTVGDIESQPFLESIRQ
                     IAAERGRHNVMFLHVSLIVSIPGTGELKSKPTQHSAKELLSLGIQPDVIICRSDAPVP
                     RDILEKISLFCNIPVENAIPNLTAPVLYEVPLMLEREGLADVVVRRLGLICHMPDLTE
                     WATMVHRAKHPQGSVEIALVGKYVALHDAYLSVAEALTHGGIENDVKVNIRWVDSETV
                     TDGNAAELLDGADGVLVPGGFGSRGIEGKIAAVRWARESRVP"
BASE COUNT         1627 a         3042 c         2499 g         1585 t
ORIGIN      
        1 gtctcctccc tgccggaagc cgtacaccgc ctccccggcc tcgtcccgga agtaagtggg
       61 gtcggtcttg gtcaggctct ccggggtaca gatccccaca tagcctacgg tggtgtcccc
      121 gtagtccagg agagcatagg gctgaaacac cggttcgccc gccgccaggc cggtgaagtt
      181 gcagctgaga taggggaact ccgccccggc cgccagctcc atcagccggt ccaccccata
      241 gtcgaactcg tggttgcccg gcaccgccag gtcatagccc gccgcgttca tcagggagat
      301 gatgtactcc ccctgggaga gggtgcccac cgcctggccc tggatggcat ctcccgcgtc
      361 caccagggtc acccggtccg ccccgtactg ctcctccagc gccccggcat aggccgccag
      421 gccggcatag ccgatggcgt cgtccagggc acagtggacg tcgttggtgt ggagaatcac
      481 gatcttctct cccgcctccg ccgcctggga ggcggaggcc agggagaccg tcagcagcag
      541 cgccgctgtc agcgccgccc atctgtccac ctttttcatg tatcatcatc ctttccgttt
      601 tcatattttc cgccctgaca gcgctcccga tacctgcccg gcgtctcgcc caccagactc
      661 ttgaaggtcc ggatgaagct gttggaggag ttgaatcccg tctggtagcc gatctccgtc
      721 acactggcgt cggtggactc cagcagcagg cgggcctgct ccacccggta ctgccgcagg
      781 tagtgggaga agcccggcgg ctggtatttg ctgaacaggc tggacagata ggacacactc
      841 agccccacgt ggccgctcac ctcgttgagg gacagcccgc tgtccgagaa gtgggcggcg
      901 atgtagtccc gggcccggtc catatagcca cgctggctgt tgtgtcccca ctcgtccagc
      961 tcagccagca gccgctcctg ctcctgccag aagtcggggg agtccgcagc gcaggcggcg
     1021 gagtatcgct cccccagttc ccgctggacc ttcagcacct gggtcagcac gcagccccgc
     1081 agcatagagc gcatctgcac cggggactga gcgcgtccta ctgcctgacg caggtccgcc
     1141 agctgggccc tggccccctc cagctctccg ctcagcgcct tctggagcat ctcccgcacc
     1201 tgggcatcat atacgcccgc acctaccgga ccggggccgg gctgcccggc ctccccgcag
     1261 caatatttcc ggtaattcag gtcctgtctg gcctcctgcc aggcggcgga gagctccagc
     1321 aggctccctc tggcctggct ggcccccacc gcggcagaga atgggaagtc ccgggtctcc
     1381 cgctccagcg cctgccggca gtcctccagg gggatggcga atccgcctgg gaccggtggg
     1441 gaagcgcaca gcaccgccga cagccctccg cccggggcgg gctggatctg ggcaaggcag
     1501 cgcccctccc aaaaccgctt caggcttctc cacaggctcc gggtgtagat ctccccctcc
     1561 accgccgagg cgctcctctc cggcaccgcc tccaccgcca gcaccaggta agtcccctcc
     1621 aggggaaagg ggctctccag agcagccagc atcctggcca cctcctccgg ctccgtctgg
     1681 cccccctcca gcagggactg gaagaaccgc tgggccagcg ccgggcgcac atcggagagc
     1741 agtccggtca gccgctggtt ctcccgctgt ccccgggcca gggcggcgct gatgagcccg
     1801 gcctcgtcct ggcccgcctc cgtctcctgc cctgtgagca tggattccac gatccgctgg
     1861 attggccggt agatcacctg gaggatcacc agggacagga tcaggatcag caggagcagc
     1921 accgccgccg cagggaggaa ggcggtgagg atctcctggg gagagggcag ccagtccccg
     1981 ctgttccaat aggtcaggca gtaccacccg gccgtctggg acctagccac acaaactaca
     2041 gtgtctctct gtccgctcaa agcgtacacg ccttcccggg gagattcctc ggtcaccatc
     2101 agctcctccc gctccgggta ggtcatccgg ttgggaaaga cgggctctcc cgccccgtca
     2161 tagacgtaca ccgggtctcc ggacaggccg ctccagccct cctggaactg ttcaaacagg
     2221 tctctggcgc tgagctggac gatgagcagt cccagctttt tgggctgtgg gaaggcgacc
     2281 agcagatata tctttccgtc ctcacaccac agggaggggg tcccgctctc cgccgccgcg
     2341 gaccaccgtt ccagctgccc aggccgctcc gggtctcccg cctccagcag cagctgccgg
     2401 tcggtgagga tctgcccact ttgatagagg taaagccagc accgctcgat cagctcgttc
     2461 ccctcccgca gcccctggat ctgggcggct acccccgagg tcctggagaa atccggcttt
     2521 tccggtgcca ccagcaggct ggagacgtca tcatccttcc agaaggtctg gatctgaagc
     2581 tccagggcct ggatggtccg gtccagggag gtgctggtcc gctggagcat ggacatggtg
     2641 ccctccatcc cctgctcccg gtagcgggac accatctctc cccggacaaa gaggtaggag
     2701 ccccccaccg tgagcaggga gaaggccagc aggatgagga cggtcctccc cagcagcttc
     2761 tgggtgcgca gccggcctac cagcgagatg cgattcatat cccttccctt tctttttcca
     2821 ctgtttttta tcacgccagc aggtcccaga ccagccggac gaccaacagg gtgagcacca
     2881 cgaagaacat ggggcggatc acctttgcgc cgcccttcag ggccagcccg gcccccacat
     2941 agccccccac gatgccgaac agggcggcgg ggatgcccac cgcccacagc accttccccc
     3001 ccagggcgaa ggtcaccagg ctggccaggt tggaggtggc gttggccacc ttggtattgc
     3061 ccgcagcggt aagcaggtcg aaccggcaca ggccggtaaa ggccagggtg agaaaggtcc
     3121 cagcccccgg accaaaaaat ccatcgtagc acccgatcac cagcccgatg gccagagaga
     3181 gaggcaccag cttccccact cccagctccc cggaccggtc ctccgtcccg aagtcccgtt
     3241 taaacagcag gaaaaccgcc atcaccggca ctacagccag catcaggtag tagagggcct
     3301 gctccggcat gatcatgttc aaacgcgccc ccagccaagc acccaccagc gccccggcac
     3361 ccccggccag ggcggagggc agatgcacgt ccccccgctt cagaaaacgc ccggtggcta
     3421 tcagtgtgcc gaagaagttg ccgcacttgt tggtgccgga ggccaggtgc gggggcagtc
     3481 ctgccagcag ataggcgggc agggtgatca gccccccgcc gccggccacc gcgtcgatca
     3541 gtccgcccag aaagaccagc ggacagacaa tgatccagat gcggatcagc tcgtcccatt
     3601 ccatgttcat ctctcctgtt cccggcctgt ggccgcctga ctattgtacc acagcttctg
     3661 cctggaatcc acatggagcg gacaagatct tccgtcccgc ccgtcaaagt tctccaattt
     3721 tctttcccct ctcccttgac gtgccgccta aacctttgta aaataaattg gtattgtgtt
     3781 gtgagagggg agtggccgtc gtggccaggg aagggtttga caatcagaaa tatttgacca
     3841 tgcagtccca gcacatccgg gaccgcatcg accagttcgg cggtaagctg tatctggagt
     3901 tcggcggcaa gctgttcgac gacttccatg ccagccgggt gctgcccggc tttcagccgg
     3961 acagcaagat ccgcatgctg gagcagctga aggacaaggc cgagatcatc atcgtcatta
     4021 acgccggcga catcgagaag aacaaggtgc ggggcgacct gggcatcacc tatgacagcg
     4081 acgtccttcg cctcatcgac gcgttccgct ccaagggcct gtatgtgggc agcgtggtag
     4141 tcacccagta caccgggcag gccgccgccg attccttccg cctgcggctg gagaagctgg
     4201 gggtgcgggt ctaccgccac tactccatcc agggctaccc ccacaacatc gcccacatcg
     4261 tcagcgatga ggggtacggc aagaacgact atatcgagac ctcccgccct ctggtggtgg
     4321 tcaccgcacc cggccccggc agcggcaaga tggccacctg cctcagccag ctctatcacc
     4381 actaccgccg gggggtgacg gcgggctacg ccaagttcga gaccttcccc atctggaacc
     4441 tccccctgaa gcacccggtc aatctggcct acgaggcagc caccgccgac ctggacgacg
     4501 tgaacatgat cgaccccttc catctccagg cctacggcga gaccaccgtc aactacaacc
     4561 gggacgtgga ggtctttccc gtcctggctg ctacctttga gaagatcacc ggcagctgcc
     4621 cctatcagtc ccccaccgac atgggggtga acatggcggg caactgcatc accgacgacg
     4681 gtgcctgccg ggaggctgcc tgtcaggaga tcgtccggcg ctactatgat gtgctctccg
     4741 accgccggca gggcagggga ggggacgacc cggtctataa actggagctg ttgatgcagc
     4801 aggccggcat cacccccgct atccgccccg tagtggacca ggccaaccgg ctggaggagg
     4861 agaccggcga gcctgccatg gccatccaac tgcccgacgg caggatggtc accggcaaga
     4921 cctccgagct gatgggctgc tccgccgccg ccctgctcaa cgccctgaag tccctggccg
     4981 ggctgggggg ccatggcgtg catctgatcg cccagtccgc catccagccc atccagactg
     5041 tcaaggtcca gtatctgggg tcaaacaacc ccaggctgca cagcgacgag gtgctcatcg
     5101 ccctggccgc ctccgccaat ctggacccca aggccgcccg ggcgctggag gccctggccc
     5161 atcttcaggg ctgtcaggcc cactgctccg tcatcctatc ccccgccgac gaggggactt
     5221 accgcaagct gggcatccag ctcacctgcg agcccaagta tcagaccaac aagctctatc
     5281 accgataagc gcaaagaggc gcccccggga gaaggatctc ccaggggcgc ttgtcctttt
     5341 ttgagaaaaa gaatggaaca aactatctgc cagagaccgt ccttatatgc gcagacctct
     5401 gggctgtctc ggctccttta gggaacaacg atgatttccc catcgaggac ctgaaattct
     5461 aagccccaga cctccccaaa aaagaggatg gccgcggaga tcccaccatg gtattccacc
     5521 gccggcatgg gaacttgtgt gaatacccca tcttttcccc gtacccgtgt ctctatttcc
     5581 tttctctccc cattgaccag cgcaagggat tctccggccg tcagggagag ggaaattccc
     5641 tggtacactc ccgtcgctct tttgtttatt tcatcccact gatattcagc tcccaatttc
     5701 ttgcacagct cttccatgct tgcggcatag gggcccgcgt actctgcgcc gccctctccc
     5761 acctcgatgg aaaagggata cagctgttcc ttcatctgca cctctccaag cggttctcca
     5821 tttagagaaa actcggctgt ccccagaatc acagatgcaa tttcggtcaa gtccatcaca
     5881 gaccgagagg tatagacgat ctctgccagc tcttttccaa atacatttgc cggtcccggg
     5941 atatcccgca tctccagctc cagtcccatc tgctttctgg tgaggatctg gccgtctttc
     6001 atccggaaga acaccagggg ggctagagag gaggcagtat ccggatattt tcctacatag
     6061 gtgaaccgtg tggggccaat ctccacatgg tccacaaata cctcttctcc gccattccag
     6121 tccacagaga gctcccgcgc aatggagacc cggaccacct ggggcatctg gtccacgggg
     6181 atctggagca cacagctctc ctccatgaat cccaggcgca ggccgatggg ctccgtctga
     6241 ggatgctcca gcccggtcag cgtgagctgg agctgccagg agcggctggt ctccgagggg
     6301 gcgggcaact cttcaaatcc gaagctggac atatggttcc cggcctccat ctggaattga
     6361 aacacggaga tgtctccctg cccctgctcc atcagattct ccggcatctc tcggccccag
     6421 gccgcccgat agcagtccgc aacgacctga ttgttcttca gattttccac cgcctgctca
     6481 tgcagcccct ccacggtgat gaccacatac agggtatcac cgtcacagat gctgctgtct
     6541 agggtcagac gataattttt atcctgcgca ctcgcccccg tctgaacaaa tggttccagc
     6601 tggctggtat ccccctgaaa aaagagctcc agcacgccga cccgcgacac gacagcggcc
     6661 cctgtcccca gaaggagcag gatgagcacg gcacacacta gaatcagcct ccctattctc
     6721 ctcctaggcc gtttcagccc tcgctccttc tgttccagca cactgatgat ccgcgccctt
     6781 gcctcttcgg aaaggcagat ctgctccaag gcgcgatgat attcctgttt catggccgcc
     6841 cctcctcttc cagccgttcc ctcagcttct ttctggcccg ctggaggcgg gtggtgaccg
     6901 ccgtctggct gatccccagc agagacgccg tctcccgggt ggagaagccc tcgtagtaaa
     6961 tgagatagag gatctcccgc tccccggcct tcagctccat cacctgccgc aggaccggct
     7021 cctcctgggg cgcggccacc gccagcgcct cctccagcgg ctcggtccgg cgcagcttca
     7081 gccgccgcca gaggctgcgg cactcgttca ccgccacccg gaacagccag gcccgctcct
     7141 ttcccggctc caggtccggc cggcgctcca gcaccttcaa aaagacggtc tgggtcacgt
     7201 cctccgcgtc ctgccgggag cccgtgcagc tccacgccag ccggaacacc gcgtctccat
     7261 accgctcaaa gagctcccgc gtatccaatg ggtgtccccc ttcccttcgt ttttctctct
     7321 gcctgtaaaa cgcccggcag cgcccatttg tcacatctgt ttttgccgag catcattttt
     7381 ctgatcttgc ccaaacgatc cccgagatat gggggaggag aggaggtccg ggacgatgcg
     7441 ttccgatgtt ttccacaaaa agcccgccgt gtccactgga gcacagaggc cgcccccgcc
     7501 ggggctgtgg accgcaccca tttttcgccc atctgtccga tttttttctt gactcgcacc
     7561 ccagacaagc ataaaataaa ccggtatttt tttcaacagg atcaaacttt gatggaggga
     7621 tcaacatggc agtgaagtac atttttgtca ccggcggcgt ggtttccggc ctgggcaagg
     7681 gcatcacggc ggcctctctg ggccgcctgc tgaagcagcg gggcctgcgg gtaaaggtcc
     7741 agaagctgga cccgtacctg aacgtggacc cgggcaccat gagcccctac cagcacgggg
     7801 aggtcttcgt caccgacgat ggggccgaga ccgacctgga cctgggccac tatgagcgct
     7861 ttatcgacga aaacctcacc gtccactcct ccgtctcctc ggggaaggtc tactggaacg
     7921 tgctcaaccg ggagcggacc ggggactatc tgggagccac ggtccagatc atcccccaca
     7981 tcaccaatga gatcaagcgg aacatttact ctctggacac cccggacact gacgtggcca
     8041 tcgtggagat cggcggcacc gtgggcgata tcgagtccca gcccttcctg gagtccatcc
     8101 gccagatagc cgccgagcgg ggacgccaca acgtcatgtt tctccatgtc tccctcatcg
     8161 tatccatccc cggcaccggg gagctgaaga gcaagcccac ccagcactcc gccaaagagc
     8221 tgctctccct gggcatccag cccgatgtca tcatctgccg cagcgacgcg ccggtgcccc
     8281 gggatatttt ggagaagatc tccctgttct gcaacatccc ggtggagaat gccatcccca
     8341 acctcaccgc cccggtgctg tacgaggtcc ccctgatgct ggaacgggag gggctggccg
     8401 acgtggtggt ccgccggctg gggctgatct gccacatgcc cgacctgacg gagtgggcca
     8461 ccatggtcca ccgtgccaag catccacagg gcagtgtgga gatcgccctg gttggcaaat
     8521 atgtggccct ccacgacgcc tatctgtcgg tggcggaggc cctgacccac gggggcatcg
     8581 agaacgacgt aaaggtgaac atccgctggg tggactcgga gaccgtcacc gacgggaacg
     8641 cggcggaact gctggacggg gcggacggcg tcctggtccc tggaggcttc ggcagccggg
     8701 gcattgaggg gaagatcgcc gccgtgcgct gggcccggga gagccgggtc ccc
//
LOCUS       DBMDSJ010000021         8477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2607_length_8477_cov_1.875119, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000021 DBMDSJ010000000
VERSION     DBMDSJ010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2607_length_8477_cov_1.875119"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="ACZPAQ_01105"
     CDS             complement(<1..1907)
                     /locus_tag="ACZPAQ_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012613.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR0902602.1"
                     /translation="MKCGRLLPLLLSLLLSVSLLAPAALARETEFFPDFPEPVPASAQ
                     DWSPADPSSFDAARQALLTAVRGEDASERSVLSLLSRMTDRFNELLTEYYFCSLDYMR
                     SPASCTQEYVRYSSVCGQAESDYLSAVQEVLNSAYGSVLVGLLGEGQAQSLLSTSPNT
                     REELALLERETALVNEYWEAVVQEPSVQSGGRTWTRAQADAAYLVGSLEEDAYLTLVG
                     ELAAVRNGVLAPIYLELVKVRNQYAASKGYDDYVQYAYQTVYGRDYTPEEAALLHRAV
                     KALLPRLESELLIAQSRLSSLSPVRLSSLEDMTQEEVLDAVEPYMDAVSSEYRELYGY
                     MRDNGLYDLDILASSSTLGVTVSLPAYRSAYILSSAQGSYLDVKTLIHEFGHFANHCL
                     AEDEQFCYDVAETHSQGLEALYLTFADSLAGQAGGDAYRAAILSDLLGMLYFCMYDEF
                     EQAVYQADGLTVSGMNRLYRSISESYGFLYSIGGEEAYDWAANSQLFEQPCYTISYVT
                     SVLNSLELLVDSAEDFNAAADTYLALVAQTDTAGYRDAVAQAGLTDMLQPGAAQDVLE
                     AVEQYFYREICGLSFPDLTGHWSARYALTCASSGLFSGDEAGNFRPDSGVTWAELLTV
                     LWQVAGAPQLSG"
     gene            complement(2109..3521)
                     /locus_tag="ACZPAQ_01110"
     CDS             complement(2109..3521)
                     /locus_tag="ACZPAQ_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR0902603.1"
                     /translation="MLNRAFRTALCAGALLLALCLPASAAQEGDFSVLVNGEAVTFTD
                     AAPLLKDGRSFLPMVETFDALGFAQGDITWDAATRSVTAAKDDTSITLTIDQKELTVT
                     RGQENAAETDTTTTDAAPFIDAASSRTYVPVGLVAGALGYNVGWDAQTSTVIIDDVDA
                     ILAANSETYAMMDRYLEYTRDLTGGTCKVEGSLAVEMELSSLMTGGIQGDYSMLQSDS
                     SAFQFSTELDVELSAPDAEVSAQIDPIDLELRGDLEEGLFYFSSDALTQMSDPSVTGL
                     WFKMDLKSMMDQLSLQTGLDYTDLLLMARAAQDMSFADSLALILREVPLTSASMTTRD
                     LLALYNSMFSDSALEQKGTTYISTTQAEDMDITFTLFTSDSKVTGYSMTMKADQEGAI
                     VSVDASMKGKELAMTMEMSTPAVIDEASGVSIPGFTYTVTMDGTYSASSTQPQTQPPA
                     GADVVDLMEQLTVEMDPVSV"
     gene            complement(3699..6329)
                     /gene="clpB"
                     /locus_tag="ACZPAQ_01115"
     CDS             complement(3699..6329)
                     /gene="clpB"
                     /locus_tag="ACZPAQ_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR0902604.1"
                     /translation="MNMNQFTQKSLAAIQGAQDIALEYGHQQIEQAHLLMALVQDGEG
                     LIPQLLTNMGLTLPSFQAAVQHEVEKLPRVSGGGREADKVYVAQDVDRALKSAESAAA
                     SMKDEYISVEHILLGLLDSANSALKELFRTYNVTREKVMQALTSVRGNQRVTSDNPEE
                     TYDALKKYGTDLVERARQNKLDPVIGRDDEIRNVIRILSRKSKNNPVLIGEPGVGKTA
                     IAEGLAQRIVKGDVPNSLKDKTIFSLDMGSLIAGAKYRGEFEERLKAVLNEVKKSEGR
                     IILFIDELHTIVGAGKTEGSMDAGNLLKPMLARGELHCIGATTLNEYRQYIEKDAALE
                     RRFQPVMVNEPTVEDAIAILRGLKERYEVYHGVKITDGAIIAAATLSNRYITDRFLPD
                     KAIDLVDEACAMIRTEIDSMPTELDVIQRKIIQHEIEEAALKKETDKLSQEHLSEIQK
                     ELSDMRDEFNAKKAQWENEKNAIGKVQKLREELEEANAQLEKAQREYDLERAAQLQYG
                     RIPELKKALEAEEQIANEGRQRSLLRDKVTEEEIARIIERWTGIPVSRLMEGEREKLL
                     HLEDILHQRVVGQDEAVRLVSEAILRSRAGIADPNKPIGSFLFLGPTGVGKTELAKTL
                     AEALFDSEKNLVRIDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILF
                     DEVEKAHPDVFNILLQVLDDGRITDSQGRTVDFKNTIIILTSNLGSQFLLDGIDSRGE
                     ISQEAREQVSDLLKRSFRPEFLNRLDEIVFYKPLTKDNVTHIIDLMAADLNRRLEDKQ
                     LTVVLTPAAKEEIIASAYDPIYGARPLRRYLQHTVETLISKKIIADQVEPGSRITVDC
                     RDGQLTVDSREVLTGEVIDA"
     gene            complement(6579..7001)
                     /locus_tag="ACZPAQ_01120"
     CDS             complement(6579..7001)
                     /locus_tag="ACZPAQ_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR0902605.1"
                     /translation="MYMIPYTRRSNSSMMDLFDELERSMFGESGRRTPVFSTDIRDEG
                     AHYLLQADLPGFRKEDIDLDVKDGVLTISAKHQADDRTAADNYICRERRTGSFSRSFT
                     LDGIQEDAIRASYQDGVLELILPKRQEVLPQCHKIQIQ"
     gene            7233..8222
                     /locus_tag="ACZPAQ_01125"
     CDS             7233..8222
                     /locus_tag="ACZPAQ_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR0902606.1"
                     /translation="MKILVFGPRDRYDVYRPPFADEMPVELVFRRPDQTYVQAAQENA
                     DARIIFVDAITDVGPDVMDQLPELKMIHSEGVGYNCIDCQAARERGIYVCNNKGCNAE
                     SVAEHTIMLMLMALRFGIPGHNAVIQGQQIQMKQKAIAAHAPGLFQCAVGLVGFGDTA
                     QATARRLASFGCKLYYYTRHRRPPQVEEDFGVTYLPLEELTAQCDIISLHCAVTDETR
                     EMVDGAFLARMKPNSILVNCGRGDLVDNQALRKALIDGTIRGAALDTIFPEPTPGDHP
                     LVDLPPEVRDRVVYSSHLGGSSGGAFAKAHLTMWTNARHILNGERPTNVVNGL"
     gene            8239..>8477
                     /locus_tag="ACZPAQ_01130"
     CDS             8239..>8477
                     /locus_tag="ACZPAQ_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HIR0902607.1"
                     /translation="MIRALRPEDLDQVMQIWLDANLEAHCFLPESYWCDHMEEVRAAL
                     PAAQVFVYQDWEGLQGFAGLTGDYLAGLFVRRAARS"
BASE COUNT         1616 a         2468 c         2779 g         1614 t
ORIGIN      
        1 ccggacagct ggggggcgcc ggccacctgc cagagcacgg tgagcagctc cgcccaggtc
       61 acaccgctgt ccgggcggaa gttgccggcc tcatccccgg aaaagaggcc ggaggaggcg
      121 caggtcaggg cgtagcgggc agaccagtgg cccgtcaggt cgggaaagga cagaccgcag
      181 atctcccgat agaaatactg ctccaccgcc tccaggacat cctgagcggc accgggctgg
      241 agcatgtctg tgagacccgc ctgagcgacg gcgtcccggt agccggctgt gtctgtctgg
      301 gccaccaggg ccagataggt gtcggcggcg gcattgaagt cctccgcgga gtccacaagg
      361 agctccaggg aattgagaac agaagtcaca tagctgatgg tgtagcaggg ctgttcaaac
      421 agctgggagt tggcggccca gtcataggcc tcctccccgc cgatggagta gaggaagccg
      481 taggactccg agatagagcg gtagagccgg ttcatcccgg acaccgtgag gccgtccgcc
      541 tgatagaccg cctgctcaaa ctcgtcgtac atgcagaagt acagcatccc tagcaggtcg
      601 gagagaatgg ccgcccggta ggcgtccccg cccgcctggc ccgccaagct gtcagcaaag
      661 gtgaggtaca gggcctccag cccctgagag tgggtctcgg ccacgtcgta gcagaactgc
      721 tcatcctcag ccaggcagtg gttggcgaag tggccgaact cgtggatcag ggtctttacg
      781 tccagatagc tgccctgggc ggagctgaga atataggcag agcgataggc gggcaggctg
      841 acagtgacgc ccagcgtgct ggagctggcc agtatgtcca gatcgtagag accgttgtcc
      901 cgcatatagc cgtacagttc cctgtattcg ctggagactg catccatata gggctccaca
      961 gcgtccagca cctcctcctg ggtcatgtcc tccagggagg agagccggac gggggacagg
     1021 gagctgaggc gggactgggc gatcagcagt tcggactcca gacggggcag aagggccttg
     1081 accgcgcgat ggaggagggc ggcctcctcc ggcgtatagt cccggccgta gacagtctga
     1141 taggcgtact ggacatagtc atcatagccc ttggaggcgg cgtactgatt gcgcaccttt
     1201 accagctcca ggtagatagg ggcgaggacc ccattgcgga cggcggccag ctcccccacc
     1261 agggtcagat aggcatcctc ctccagtgag cccaccagat aggcggcgtc cgcctgggca
     1321 cgggtccagg tcctgccgcc ggactggaca gagggctcct ggaccaccgc ctcccagtat
     1381 tcgttgacca gagcagtctc acgctccagc agggccagct cctcccgcgt attgggagag
     1441 gtggacagga gggactgagc ctgcccctcc cccagcagcc cgaccagaac ggagccgtag
     1501 gcgctgttca gcacctcctg gacggcggag aggtagtcgc tctccgcctg accgcagacg
     1561 gagctgtaac ggacatactc ctgggtgcag gaggcggggg agcgcatata gtccagggag
     1621 cagaagtagt actcggtgag cagctcgttg aagcggtccg tcatcctgga cagcagagag
     1681 aggaccgagc gctctgaggc atcctccccc cgaacggcgg tcagaagtgc ctgccgggcc
     1741 gcatcaaagc tgctggggtc ggcggggctc cagtcctggg ccgaggcggg gaccggctcc
     1801 gggaagtccg ggaagaactc ggtctcccgg gccagggccg ccggagcgag cagcgagaca
     1861 gacagaagga gggagagcag gagtggcaga agacggccgc acttcataga gatgcctcca
     1921 atacagacga ttttgggccc ggaagcctgt ctccatccta tcatatccgg ggtggaaaga
     1981 caagcgccag gccaaggggc agagctgaaa aatacgccgc ggaaggggaa aacagcgtca
     2041 aaaaggcccg ctcctgtatg agagatgtca cacgggagcg ggccgggggc tgatggtccg
     2101 ggggcggctc agacggagac cggatccatc tcaacggtca gctgctccat caggtccacc
     2161 acgtcggcgc cggcgggggg ctgggtctgg ggctgggtgc tggaggcgga gtaggtgccg
     2221 tccatcgtca cggtgtaggt gaagccggga atggagacgc cggaggcctc gtcgatgacg
     2281 gcgggcgtgc tcatctccat ggtcatagcc agctctttgc ccttcatgga ggcgtccacg
     2341 gacacaatgg ccccctcctg gtccgccttc atggtcatgg aatagccggt gaccttggag
     2401 tcggaggtga acagagtaaa ggtgatgtcc atgtcctccg cctgggtggt ggagatataa
     2461 gtggtcccct tctgctccag ggcactgtcg gagaacatgc tgttataaag agccaggaga
     2521 tcacgagtgg tcatggaggc ggaggtgagg gggacctccc gcaggataag ggccagagag
     2581 tcggcaaagc tcatgtcctg agcggcccgg gccatgagca ggaggtcggt gtagtccagg
     2641 ccggtctgga gggagagctg gtccatcatg gacttcagat ccatcttaaa ccacaggccg
     2701 gtgacgctgg gatcggacat ctgggtcagt gcatcggagg aaaagtagaa caggccctcc
     2761 tccaggtcgc cccgcagttc caggtcgatg ggatcgatct gggcggatac ctcagcatca
     2821 ggagcggaca gttccacatc cagctctgtg ctgaactgga aggcggagct gtcgctctgg
     2881 agcatggagt aatctccctg gatgccgccg gtcatgaggg aggagagctc catctccact
     2941 gccagactgc cctccacctt gcaggtgccg ccggtcagat cccgggtgta ctccagatac
     3001 cggtccatca tggcgtaggt ctcggagttg gcggccagga tggcatctac gtcgtcgatg
     3061 atgacagtag aagtctgggc atcccagcct acgttgtacc ccagggcacc ggccaccagt
     3121 cccacgggga cgtaggtgcg ggaggaggcg gcgtcgatga agggagcggc atcggtggtg
     3181 gtggtgtccg tctcagcggc gttctcctgc ccacgggtca cagtgagctc cttctggtcg
     3241 atggtcaggg tgatggaggt gtcatccttg gccgcggtga cactgcgggt ggcggcgtcc
     3301 caggtgatat ccccctgggc gaagcccagg gcgtcaaagg tctccaccat gggcaggaaa
     3361 gaacggccgt ccttcagcag gggggcggcg tcggtaaagg tgacggcctc cccattgacc
     3421 aggacggaga aatccccctc ctgggccgcg gaggcgggca ggcagagcgc cagaagcagg
     3481 gcgccggcgc acagggcggt gcggaacgcg cggtttaaca tgacagatcc actccttaaa
     3541 attgacatgg gccgccaggc ggcccgaaag ccgagctttt ttccggtctc aatatagcat
     3601 atccgggggg aaaagacaag gagcgagagg gagaaggaag aaaaaatccg gcgcgggccc
     3661 gcaggtccgc gccgaaaaaa ggaggaatag gggaggaatc aggcgtcgat gacctcgccg
     3721 gtgagcacct cccggctgtc cacggtgagc tgtccatccc ggcagtccac ggtgatgcgg
     3781 gagccgggct ccacctggtc ggcaatgatc ttcttgctga taagggtctc caccgtatgc
     3841 tggagatagc ggcgcagggg ccgggcgccg tagatggggt cgtaggcgct ggcaatgatc
     3901 tcctccttgg ccgcgggggt gaggaccacg gtgagctgtt tgtcctccag ccggcggttc
     3961 aggtcggcgg ccatcagatc gatgatatgg gtcacgttgt cctttgtcag gggcttgtaa
     4021 aacacgatct catccagccg gttgagaaac tccgggcgga aggaccgctt gagcagatcg
     4081 ctgacctgct cccgggcctc ctggctgatc tccccccggc tgtcgatgcc gtccagcagg
     4141 aactggctgc ccaggttgga ggtaagaatg atgatagtgt tcttgaaatc cacggtgcgg
     4201 ccttgactat ctgtaatacg gccgtcgtcc aatacttgaa gtaatatatt gaatacgtcc
     4261 gggtgggcct tttccacctc atcaaagagg ataacggagt agggtttccg ccgcaccgct
     4321 tcagtgagct ggccgccttc ctcatagccc acatatccgg gaggagcccc gatgagccgg
     4381 gacacggaga atttctccat atactcgctc atgtcgatgc gaaccaggtt cttctcgctg
     4441 tcgaacaggg cctcagccaa ggtcttggcc agttcggtct taccaacgcc ggtggggccc
     4501 aggaacagga aggagccaat gggcttgttg gggtcagcga tgccggcccg gctgcggagg
     4561 atggcctcgg acaccaggcg taccgcctcg tcctgaccca ccacccgctg gtggaggatg
     4621 tcctccaggt gaagcagctt ctcacgctcc ccctccatga gccgggagac ggggattccg
     4681 gtccaccgct cgatgatgcg ggcgatctcc tcctcggtga ccttgtcccg cagcagggaa
     4741 cgctgccgcc cctcattggc gatctgctcc tccgcctcca gggccttttt cagctcgggg
     4801 atgcggccgt actggagctg ggcggcccgc tccaggtcgt actcccgctg ggccttctcc
     4861 agctgggcgt tggcctcctc cagctcctcc ctgagcttct ggaccttgcc aatggcgttc
     4921 ttctcgttct cccactgggc tttcttggcg ttgaactcat cccgcatgtc ggagagctcc
     4981 ttctggatct ccgacagatg ctcctgggac agcttatctg tctctttttt cagagcggcc
     5041 tcctcgatct cgtgctggat gatcttccgc tggatgacgt ccagctcggt gggcatggag
     5101 tcgatctcgg tgcggatcat ggcgcaggcc tcatccacaa gatcgatagc cttgtcgggg
     5161 aggaagcggt cagtgatata gcggttggag agagtggcgg cggcgatgat ggccccgtcg
     5221 gtgatcttga cgccgtgata gacctcgtac cgctccttca ggccgcggag gatggcgatg
     5281 gcatcctcca ccgtgggctc attcaccatg acaggctgga agcggcgctc cagagcggcg
     5341 tccttttcaa tatactgccg gtactcgttg agggtcgtgg cgccgataca gtgcagctct
     5401 ccccgggcca gcatgggctt gaggagattg cccgcatcca tggagccctc cgtcttgccc
     5461 gcccctacga tggtgtgcag ctcgtcgata aagaggatga tacgcccctc ggacttcttc
     5521 acctcgttga ggacggcctt cagccgctcc tcaaactcgc cccggtactt ggccccggcg
     5581 atgaggctgc ccatatccag ggagaagatg gtcttgtcct tcaaactgtt gggcacgtcc
     5641 cccttaacga tccgctgggc cagcccctcg gcaatggcgg tcttacccac gccgggctcc
     5701 ccgataagga cgggattgtt tttgctcttg cgggagagga tgcggatgac attgcggatc
     5761 tcgtcgtccc ggccgatgac cgggtccagc ttgttctggc gggcccgctc caccaagtcg
     5821 gtgccgtact tcttcagggc gtcataggtc tcctcagggt tgtcactggt gacccgctgg
     5881 ttgccccgga cagaggtcag ggcctgcatc actttttccc gggtgacgtt gtaggtgcgg
     5941 aacagctcct tcagggcgct gttggcactg tccagcaggc ccagcaggat gtgctccaca
     6001 gagatatact catccttcat ggacgccgcg gcggactcgg cggatttcag ggcacggtcc
     6061 acgtcctgag ccacatagac cttgtccgcc tcccggcctc caccggagac ccggggcagc
     6121 ttctccacct cgtgctggac ggcggcctgg aagctgggga gggtcaggcc catattggtg
     6181 agcagctggg ggatcagccc ctcgccgtcc tggaccagcg ccatgagcag atgggcctgc
     6241 tcgatctgct ggtgcccgta ctccagggcg atgtcttggg cgccctgaat ggcggccagc
     6301 gatttctggg taaattgatt catattcata aaaggaacac ctcctagatc cgatctatat
     6361 tgattcttaa actttagcac tcaataaact taactgctaa aattagtata gagccattgg
     6421 acaagatttg caagtctttt ttcaagtttc catacttttg tgtgctgcgt cctacagggg
     6481 cacacaaact gaaaattgag tttgggaaga aacgcgattt ggaaaagagt caggcggagc
     6541 cggcgtaccg gctccgcctg accgcggctg gggagacctc actggatctg gatcttgtgg
     6601 cactggggaa ggacctcctg ccgcttgggc agaatgagct ccagcacccc gtcctgatag
     6661 gaggcccgga tggcgtcctc ctggatgccg tccagggtga aggaccggga gaagctgccg
     6721 gtgcggcgct cccggcagat gtagttgtcg gcggcggtgc ggtcatcggc ctggtgcttg
     6781 gcggagatgg tgagcacgcc gtcctttaca tccagatcga tgtcctcctt gcggaagccg
     6841 ggcaggtcgg cctggagcag gtagtgggcg ccctcgtccc ggatatcagt gctgaagacc
     6901 ggcgtgcggc ggccgctctc gccgaacatg ctgcgctcca actcgtcaaa cagatccatc
     6961 atgctgctgt tgctgcggcg ggtgtagggg atcatataca taagtcaaaa ctcctttcgg
     7021 tcccgggcgg agcatcctcc ggcccgggcc tctctgaact ttggctttat tatagtgagg
     7081 gattatgaat tttttgttac acagatgtgt cacaagtgta aatattagca ctcaataaat
     7141 aagagtgcta atattttgca gaaaggctcc ccttgtattg gcgaaaaacg ggtggtataa
     7201 ttgtcataca attaaaataa ggagggcacc ccatgaagat attagttttt ggccccagag
     7261 accgctatga cgtgtaccgg cccccatttg cggatgagat gccggtggag ctggtgttcc
     7321 gccgcccgga ccagacctat gtccaggcgg cccaggaaaa tgcggacgcg aggatcattt
     7381 ttgtggatgc catcacggat gtgggtcccg atgtgatgga ccagcttcca gagctgaaga
     7441 tgatccactc tgagggtgta gggtacaact gcatcgactg ccaggccgcc cgggagcggg
     7501 ggatctatgt gtgcaacaat aaggggtgca acgcggaatc ggtggcggag cacaccatca
     7561 tgctgatgct gatggctctg cgcttcggca tccctggaca caacgcggtc atccaggggc
     7621 aacagatcca gatgaagcag aaggccatcg ccgcccacgc ccccggcctg ttccagtgtg
     7681 cggtggggtt ggtgggcttt ggagacaccg cccaggccac cgcccggcgg ctggccagct
     7741 tcggctgcaa gctatactat tacacccgcc accgccgtcc gcctcaggtg gaggaggact
     7801 ttggggtgac ctatctgcct ctggaggagc tgaccgccca gtgcgacatc atctcgctcc
     7861 actgcgcggt caccgacgag acccgggaga tggtggatgg ggccttcctg gcccggatga
     7921 agcccaactc catcttggtc aactgcggcc ggggcgacct ggtggacaac caggccctgc
     7981 ggaaggcact catagacggg accatccggg gcgcggcgct ggatactatt ttcccggagc
     8041 ccacccccgg cgaccaccct ctggtggacc tgccgccgga ggtcagagat cgggtggttt
     8101 acagctccca cctggggggc agctccgggg gggcttttgc caaggcgcat ctgaccatgt
     8161 ggaccaacgc ccggcatatt ttgaacggag agcggcccac caacgtagtc aacgggctgt
     8221 aagagggggg gcggcattat gatccgggct ctccgtccgg aggatctgga ccaggtgatg
     8281 cagatctggc tggacgccaa cctggaagcc cactgctttc ttcctgagag ctattggtgt
     8341 gaccatatgg aggaggtgcg ggcggcgctg cccgccgccc aggtgttcgt ctatcaggac
     8401 tgggaggggc tccagggctt tgcgggactg accggcgact atctggcggg gctcttcgtc
     8461 cggcgtgccg cccggtc
//
LOCUS       DBMDSJ010000022         8461 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2610_length_8461_cov_2.415550, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000022 DBMDSJ010000000
VERSION     DBMDSJ010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2610_length_8461_cov_2.415550"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="ACZPAQ_01135"
     CDS             complement(<1..130)
                     /locus_tag="ACZPAQ_01135"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF014613.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A/cobalt-precorrin-6A reductase"
                     /protein_id="HIR0902608.1"
                     /translation="MSILIFGGTTEGRLLARELSRRGLPAWVSVATPLGAEELSGLP"
     gene            complement(127..861)
                     /gene="cobJ"
                     /locus_tag="ACZPAQ_01140"
     CDS             complement(127..861)
                     /gene="cobJ"
                     /locus_tag="ACZPAQ_01140"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR0902609.1"
                     /translation="MKRLYVIGMGPGGAEQLTPQALRALEDSRVVCGYTTYMDLAREF
                     LEGKELVTTLMTRELERCRIALERAEAGETTAMICSGDAGVYGMAGPILQMAEEFPEV
                     EIEVVPGITAALSGAAVLGAPLMHDFAVISLSDLLTPWEVIEKRLDCAGAGDFVVCLY
                     NPMSRRRRDHLARACAILRRHRPGTTVCGWVKNIGRAGEERRLLTLEELEGEQVDMFT
                     TVFIGSSSTQQLSGRMVTPRGYEAKA"
     gene            complement(858..1856)
                     /locus_tag="ACZPAQ_01145"
     CDS             complement(858..1856)
                     /locus_tag="ACZPAQ_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR0902610.1"
                     /translation="MRVELIAFTARGNALAERLAAELTGAGCPAACTREGLRVEEWAA
                     RAFGRSEALVFVGAAGIAVRAIAPCLRHKSTDPAVVVVDEAGRYAVPILSGHLGGAND
                     LARRIAALTGAEAVLTTATDVNGVFAADQWARRQGLLVPWPERILGVSSRLLAGETVS
                     FWSRWPVAGPCPAGLNWGERDRADIVVDWTPPRPEALWLCPRTLRVGVGCRRGTSEEA
                     VQTLFDQTMNGLGLPARAVGAVCSIDLKAEEPGLLAFAGALGLPLTVYSAGRLARVEG
                     SVSPSSFVAQVTGVDNVCERAALAEGGVLLKSKTALDGVTLALAGTEPNLDWRERA"
     gene            complement(1853..2629)
                     /gene="cobM"
                     /locus_tag="ACZPAQ_01150"
     CDS             complement(1853..2629)
                     /gene="cobM"
                     /locus_tag="ACZPAQ_01150"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR0902611.1"
                     /translation="MVHFVGAGPGGPDLITLRGAELLGRADCVVYAGSLVNPALLGLV
                     REGVPVYNSAQMTLEQVLEVLEETHRRGGDTVRLHTGDPCLYGAIREQMDALDARDIP
                     WDVTPGVSSFCGAAAAAGEEYTLPGVSQSVIITRMAGRTPVPEGEDIASLAAHGATMV
                     IFLSAGMLEALSEELQKGAYTADTPAALVYKATWPEEKVVRCTVGALAQAGREHRISK
                     TALVLVGDFLAHSYRRSLLYHPGFSTEYRQGDGSAEADRP"
     gene            complement(2719..3411)
                     /gene="cobI"
                     /locus_tag="ACZPAQ_01155"
     CDS             complement(2719..3411)
                     /gene="cobI"
                     /locus_tag="ACZPAQ_01155"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR0902612.1"
                     /translation="MADKLGTFYGVGVGPGDPELLTLKAVRVLESCPVLAAPQMASGE
                     MTALSIARQAARLEGKTLLPLRFTMSRDRAEQAEGHRRAADAVRPYLEAGQDVAMAIL
                     GDVSIYSTYCYMMELLKAEGFPCVMVPGVPSFCAVAARLGRSLTEPDQPLHIIPGGGP
                     GLGEALDLPGSKVLMKAGRNHQKLLEELEARKLLDRAAMVENCGLPEERPFADLTGKP
                     DSNGYFTTVIIP"
     gene            complement(3396..4538)
                     /gene="cbiD"
                     /locus_tag="ACZPAQ_01160"
     CDS             complement(3396..4538)
                     /gene="cbiD"
                     /locus_tag="ACZPAQ_01160"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR0902613.1"
                     /translation="MPFEHYVTAGGKRLRCGYTTGTCAALAAAAAARLLLTGRAPETV
                     SLRTPKGWVVEVPVEAPRLEAGAACCCVTKDGGDDVDATHGLPVCARVEAAAGEAVLL
                     QGGPGVGRVTRPGLDQPMGEWAINRVPRQMIREAVDAVRRELDEPGGLRVTISVPGGE
                     AAARRTFNPRLGVEGGISILGTSGIVEPMSVQAIVETTALELRQAAAGGARQLVLTPG
                     NYGMEFLARGGVALPAEVPVVRCSNFIGEALDGAGAEGFSAVLLVGHIGKLVKLAGGI
                     MNTHSRWADGRAELFCAHAALCGADRDTAQALMDSATADQCLSVLERAGLREPVMDSL
                     LEAVQRHLERRAAEQFSVGAVLFSNQYGLLGMTRGAKEILEAWQTS"
     gene            complement(4528..5319)
                     /locus_tag="ACZPAQ_01165"
     CDS             complement(4528..5319)
                     /locus_tag="ACZPAQ_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR0902614.1"
                     /translation="MRQVLLAVSFGTSVEEARALDLAGVERALRRAAPELPFDRAYTS
                     PTVRRILARRGERVPGLEEALESQAAAGTEELYVVPTHVLPGAEYDRVAETAARFRPR
                     FSALHLGRPLLWDTAGVRALAQVLDSLWPAVPGTAAVLVGHGSGHPGSMAYPALQGVL
                     ELMGRSDLVVGTVEGRPDQAAVLKRLEALEVERVILLPLLLAAGVHAREDIAGPQPDS
                     WISTLKAAGFQTWARLEGMGRLPQIQALYQAGLERLREGAADRAL"
     gene            complement(5360..6127)
                     /locus_tag="ACZPAQ_01170"
     CDS             complement(5360..6127)
                     /locus_tag="ACZPAQ_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0902615.1"
                     /translation="MELREVTAGYGGADVVKGISLRFQPGEVTVLAGPNGCGKSTLLR
                     AAARLLPLSGGQVLVDGADTAGWSSRTFARQAALLPQSRPMPEITVEALVLHGRFPYL
                     GYPRRYGPADRAAARSAMERAGVLELAERNVARLSGGQRQKVYLAMALAQDTPVLLLD
                     EPTTFLDIGCQLELARLMRETAAEGKAVVMVLHDLNLALGCAHRVAVMEEGRLRAWGT
                     PAEIYAGGALEQVFGVKVHRTELEDGTEQYLFTRRGP"
     gene            complement(6127..7125)
                     /locus_tag="ACZPAQ_01175"
     CDS             complement(6127..7125)
                     /locus_tag="ACZPAQ_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR0902616.1"
                     /translation="MEAARRHKGLLMLALLAGTAAAALLSLCVGSVSLSLGEVAAALL
                     GRGQGVSGSIVLYARLPRMLAALVCGCALAVGGVIIQGVLGNPLAGPNIIGVNAGAGL
                     GAILCSALLPASMTAVPAAAFAGALCACVLVYTLARRTGASRITLVLAGVAISSILSA
                     AIDAVSILFPEAALGANAFMVGRLAGVTLEMLRLPALLTVLASGAALALSYELDLLSL
                     GDEVAQSVGLSTRLARSALLTLAAVLCGAAVSFAGLVGFVGLLVPHAARFLVGTRHRD
                     LLLASMLLGALLVTVCDLLGRVLFAPFELPVGILLSLLGGPFFLWLLFRQRGGRRL"
     gene            complement(7103..8080)
                     /locus_tag="ACZPAQ_01180"
     CDS             complement(7103..8080)
                     /locus_tag="ACZPAQ_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0902617.1"
                     /translation="MRRLFTVLAAAVLLLTACAPGEGDASASAPTSSAGVEQSAQGTC
                     IFTDSLGNTVELEQPPKRVAALLGSYAETWLLAGGEVAAVTQDAYDERGLELPEDTVN
                     LGANQQPDLEALLAAEPDLVLLTPDLDGQMGLKDSLAAAGIPAAWFKVETFDDYLNML
                     KICTDLTGRSDLYQKNGLDIQSGIDAAIASVPEGEDPTVLLLRAYSSGVRAKNSDNIA
                     GAMLKDLGAVNIADSNSGLLEDLQMESILAADPEFIFVTTMGASQEAALKSLDELLHS
                     DPAWQTLTAVKEGRVEVLPKDLFHYKPNARWGESYQMLAELMYGSGETA"
     gene            8286..>8461
                     /locus_tag="ACZPAQ_01185"
     CDS             8286..>8461
                     /locus_tag="ACZPAQ_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015956.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR0902618.1"
                     /translation="MEQQQSYSPRTLQDLVPGQSGMILSVGNQSGAVKRRLVDMGLTP
                     GTTVKVTKVAPLGDP"
BASE COUNT         1383 a         3254 c         2617 g         1207 t
ORIGIN      
        1 ccggcaggcc ggacagctcc tccgccccca ggggggtggc cacgctgacc caggcgggga
       61 ggccgcgccg ggacagctcc cgggccagca ggcggccctc ggtggtgccg ccgaagatca
      121 ggatgctcat gccttggcct cgtatcccct gggggtgacc atccgtccgg aaagctgctg
      181 ggtggaggac gatccaatga acacggtggt gaacatatcc acctgctccc cctccagctc
      241 ctccaaggtc agcagacggc gctcctcccc cgcccgtccg atgttcttca cccagccgca
      301 caccgtcgtc cccggccggt gcctccgcag gatggcacag gcccgggcca gatggtcccg
      361 gcggcggcgg ctcatggggt tatagagaca gaccacaaag tcccccgccc cggcgcagtc
      421 cagccgcttt tcgatcacct cccaaggggt gagcaggtcg gacagggaga tcaccgcaaa
      481 gtcgtgcatc agcggcgctc ccagcacagc cgctccggac agggccgccg tgatccccgg
      541 gaccacctcg atctccacct ccgggaactc ctccgccatc tggaggatgg gtccggccat
      601 gccatacacc ccggcgtccc cgctacagat catggcggtg gtctctcccg cctccgcccg
      661 ctccagggca atgcggcagc gctccagctc ccgggtcatg agggtggtca ccagctcctt
      721 accctccaaa aactcccgtg ccagatccat ataggtggta tagccgcaca ccacccggct
      781 gtcctccagg gcccgcagcg cctgaggcgt cagctgttcc gccccgccgg ggcccattcc
      841 gatcacatag agccgcttca tgcacgctcc ctccaatcca agttcggttc cgtacccgcc
      901 agggccaggg tcaccccgtc cagggcggtc ttgctcttca acagcacacc gccctccgcc
      961 agagcggccc gttcgcacac attgtccacc ccggtgacct gggccacaaa ggaggagggg
     1021 gacacgctcc cctccacccg ggccagccgc ccggcggaat agaccgtcag aggcagcccc
     1081 agtgccccgg caaaggccag cagccccggc tcctccgcct tcaggtcgat ggagcacacc
     1141 gcccccaccg cccgggccgg gaggcccagg ccgttcatcg tctggtcaaa cagggtttgg
     1201 acggcctcct ctgaggtccc ccgcctgcac cctacgccca cgcgtagggt ccgggggcac
     1261 agccacaggg cctccgggcg tggcggcgtc cagtccacca cgatgtccgc ccggtcccgc
     1321 tccccccagt tcagtccggc gggacagggc ccggccaccg gccaccggga ccagaaggac
     1381 accgtctccc ccgccagcag gcgggaggac acccccagga tccgctccgg ccaggggacc
     1441 agcaggccct gccgccgcgc ccactggtct gccgcaaaga ccccgttgac atcagtggcg
     1501 gtggtgagca ccgcctccgc cccggtcagg gcggcgatcc ggcgggccag gtcgttggcc
     1561 ccgcccaggt ggccggacag gatgggcacc gcgtaccgcc cggcctcgtc caccaccacc
     1621 accgccggat cggtgctctt gtgacgcagg cagggggcta tggcccggac agcaatgccc
     1681 gccgccccca caaagaccag ggcctccgac cgcccgaagg cccgggccgc ccactcttcc
     1741 acccggaggc cctcccgggt gcaggcggcc ggacagcccg cgccggtcag ctccgccgcc
     1801 agccgctccg ccagggcgtt cccccgggca gtgaaggcga tgagctctac cctcatggcc
     1861 ggtccgcctc tgctgagccg tccccctggc ggtactctgt ggagaagccc gggtgataca
     1921 gcaggctgcg ccggtagctg tgggccagga agtcccccac cagcaccagg gcggtcttgg
     1981 agatccggtg ctcccggccc gcctgggcca gggcgcccac ggtacagcgg accaccttct
     2041 cctccggcca ggtggcctta tacaccagcg ccgcgggggt atccgccgta taggctccct
     2101 tttgcagctc ctccgacaat gcctccagca tcccggcgga caggaagatc accatggtgg
     2161 ccccatgggc ggccagggag gcgatgtcct ccccctcagg caccggggtg cgccccgcca
     2221 tccgggtaat gatgaccgac tggctcaccc ccggcagggt gtactcctcc cccgccgcgg
     2281 cggccgcccc acagaaggag gacacccccg gcgtcacatc ccagggaatg tcccgggcgt
     2341 ccagggcgtc catctgctcc cggatggccc catagaggca gggatccccg gtgtggaggc
     2401 ggacggtatc tcccccccgg cggtgggtct cctccagcac ctccagcacc tgctccaggg
     2461 tcatctgggc gctgttgtac accgggacgc cctcccggac cagacccagc agggccggat
     2521 tcaccaggct cccggcatac accacacagt ctgcccggcc cagcagctcc gccccccgaa
     2581 gggtaatcag atcgggccct ccaggccctg cccccacaaa atgcaccata tccgttctcc
     2641 ttatctcctc agtccgggtc gctccggtac acccgcccaa cgtggaagtc ccggcgaagc
     2701 gccaccggat ttcatatctt atggtattat gaccgtggta aagtacccat tggaatcagg
     2761 tttccccgtg agatccgcaa aaggccgctc ctccggcagg ccgcagttct ccaccatagc
     2821 cgcccggtcc agcagcttcc gggcctccag ctcctccagc agcttctggt ggttcctgcc
     2881 cgccttcatc agcaccttgc tccccggcag gtccagcgcc tcgcccagcc ccgggccgcc
     2941 gccggggatg atgtggagag gctggtccgg ctctgtcagg ctccggccca gccgggcggc
     3001 cactgcgcag aagctgggca caccgggcac catcacacag ggaaaaccct ccgccttcag
     3061 cagctccatc atatagcagt aggtggagta gatggacaca tctcccagga tcgccatggc
     3121 cacatcctgg ccggcctcca gataggggcg caccgcgtcc gccgcccgcc ggtgtccctc
     3181 cgcctgctcc gcccggtccc gggacatggt aaagcgcagg ggcagcaggg tcttgccctc
     3241 cagccgggcc gcctgccggg cgatgctcag ggcggtcatc tccccggagg ccatctgggg
     3301 cgcggccagc accggacagg actccagcac ccgcaccgcc ttcagggtca gcagctccgg
     3361 gtccccgggc cccaccccca caccgtaaaa ggttcctagc ttgtctgcca tgcttccaag
     3421 atctcctttg caccccgggt catccccaaa aggccgtatt gattgctgaa cagcaccgca
     3481 cccacagaga actgctccgc cgcccgccgt tccaaatggc gctggaccgc ctccagcagg
     3541 gaatccatca ccggctcccg aagtcccgcc cgctccagca ccgaaaggca ctggtcggcg
     3601 gtggcgctgt ccatcagcgc ctgagcggtg tcccggtccg ccccgcacag ggcggcgtga
     3661 gcgcagaaca actccgcccg tccgtccgcc cagcgggagt gggtgttcat aatgcccccg
     3721 gccagcttca ccagcttgcc gatgtggccc accagcagga ccgcagagaa gccctccgct
     3781 ccggccccgt ccagggcctc cccgataaag ttggaacagc ggaccacagg gacctcagca
     3841 ggcagggcca caccgccccg ggccaggaac tccatgccgt aattcccggg ggtgagcacc
     3901 agctgcctgg ccccgccggc ggcggcctgc cgcagctcca gggccgtggt ctccacaatg
     3961 gcctggacgc tcatgggctc cacaatgccg gaggtgccca gaatggagat cccgccctcc
     4021 acccccaggc gggggttgaa ggtcctgcgg gcagccgcct cacctcccgg gacggagatg
     4081 gtgacccgca gcccgccggg ctcgtccagt tcccgccgga cggcgtccac cgcctcccgg
     4141 atcatctgtc ggggcacgcg gttgatggcc cactccccca tcggctggtc caggccgggg
     4201 cgggtgaccc ggcccacccc ggggccgccc tggagaagga ccgcttcccc tgccgccgcc
     4261 tccacccggg cgcagacggg cagaccatgg gtggcgtcca catcgtcgcc gccgtccttg
     4321 gtcacacagc agcaggccgc ccccgcctcc agacggggcg cctccaccgg gacctccacg
     4381 acccatccct tgggggtgcg cagggacacc gtctccgggg cgcgtccggt gagcagcagc
     4441 cgggcggccg cagcagccgc cagggcggcg caggtgccgg tggtatagcc gcagcgcagc
     4501 cgctttcctc cggcggtcac atagtgctca aagggcacgg tccgccgccc cctcccgcag
     4561 ccgctccagc cccgcctgat acagggcctg gatctggggc agacgcccca tgccctccag
     4621 ccgcgcccag gtctgaaatc cggcggcctt cagggtgctg atccagctgt ccggctgggg
     4681 gccagctata tcctcccggg cgtggactcc cgccgccagc agcaggggca gaaggatgac
     4741 ccgctccact tccagggcct ccagccgctt cagcaccgcc gcctggtcgg gcctgccctc
     4801 caccgtgccc accaccagat cggagcggcc catcagctcc agcaccccct gaagggcggg
     4861 atacgccata ctgcccggat ggccgctccc gtggcccacc aggaccgccg ccgttcccgg
     4921 gaccgcaggc cagaggctgt ccagcacctg ggccagggcc cgcacccccg ccgtatccca
     4981 cagcaggggc cggcccaggt gcagcgcaga aaagcggggg cggaaccggg ccgcagtctc
     5041 cgccacccgg tcgtactccg cgccagggag cacatgggtt gggaccacat acagctcctc
     5101 tgtgccagcg gccgcctggg actccagcgc ctcctccagg cccgggaccc gctctccccg
     5161 ccgcgccagg atccgccgca ccgttgggct ggtataggcc cggtcaaacg ggagctccgg
     5221 ggcagcacgg cgcagggccc gctccacccc ggccagatcc agggcccggg cctcctccac
     5281 agaggtccca aagctgactg ccagcagcac ttgtctcatc tctcatcctc ctgccgctgg
     5341 ttgcggcgct cttccgggct cagggccctc tgcgggtgaa cagatactgc tccgtcccgt
     5401 cctccaactc tgtgcggtgg accttcacgc caaagacctg ttccagcgct ccgccggcat
     5461 agatctccgc cggggtcccc caggcccgga gccgcccctc ttccatcacc gccacccggt
     5521 gggcgcagcc cagcgccagg ttcaggtcat ggagcaccat gaccaccgcc ttgccctccg
     5581 ccgccgtctc ccgcatcagg cgggccagct ccagctgaca gccgatgtcc aggaaggtgg
     5641 tgggctcatc cagcaggagc accggggtgt cctgggccag ggccatggcc agatagacct
     5701 tctgtctctg gccgccggac aggcgggcca cattccgctc cgccagctcc agcacccccg
     5761 cccgctccat ggcagaccgg gccgcagccc ggtccgccgg gccatatcgc cggggatagc
     5821 ccagataggg gaagcgcccg tggagcacca gggcctccac ggtgatctcc ggcatcggcc
     5881 gggactgggg cagcagggcc gcctgccgtg caaacgtccg gctgctccag ccggcggtgt
     5941 ccgccccgtc caccaggacc tgtcccccgc tcagagggag cagccgggcc gcagcccgga
     6001 gcagtgtgga cttgccgcag ccgttgggcc ccgccagcac ggtgacctcc ccgggctgaa
     6061 agcgcaggga gatccccttt accacatcgg cgccgccgta gcctgcggtc acctctctca
     6121 attccatcac agccgccgcc cccctctctg gcgaaacagg agccacagga agaagggccc
     6181 gcccagcagg gacagcagga tgcccaccgg gagctcaaag ggggcgaaga gcacccggcc
     6241 cagcaggtcg cacacggtca ccagcagcgc ccccagcagc atggatgcca aaagcagatc
     6301 ccggtgccgg gtccccacca ggaaccgggc ggcgtggggg accagcaggc ccacaaagcc
     6361 aaccagcccg gcaaagctca ccgccgcgcc gcacagcacc gccgccaggg tgagcagggc
     6421 gctccgggcc aggcgggtgg acaggcccac cgactgggcc acctcgtccc ccagggagag
     6481 caagtccagc tcatagctca gggccagggc cgcgccggac gccagaacgg tgagcagggc
     6541 gggcagcctc agcatctcca gcgtcacccc cgccagacgc cccaccataa aggcgttggc
     6601 ccccagggcg gcctctggaa agaggatgga gactgcgtca atggcggcgg acaggatgct
     6661 gctgatggcc acaccggcca gcaccagggt gatccggctg gcccccgtcc ggcgggccag
     6721 ggtgtacacc agcacacagg cgcacagcgc ccccgcgaag gccgccgcag gcaccgccgt
     6781 catggaggcg ggcagcaggg cggagcacaa aatggctccc agccccgccc cggcgttgac
     6841 cccgatgatg ttgggccctg ccagaggatt gcccagcacc ccctggatga tgacgccccc
     6901 caccgccagg gcacagccgc acaccagggc ggccagcatc cggggcaggc gggcatacag
     6961 tacgatggag ccgctcactc cctggccccg gcccagcagg gcggcggcta cctctcccag
     7021 cgagaggctg accgagccta cgcacagact cagcagggcg gcggcggcgg tcccggccag
     7081 cagcgccagc atcagaaggc ccttatgccg tctcgccgct tccatacatc agctccgcca
     7141 gcatctgata gctctcgccc cagcgggcgt tgggcttata gtggaacagg tcctttggca
     7201 gcacctccac ccggccctcc ttcactgcgg tgagggtctg ccaggccggg tcgctgtgga
     7261 gcagctcgtc caggctcttg agggcggcct cctgactggc ccccatggtg gtgacaaaga
     7321 tgaactctgg gtcggcggcc aggatggatt ccatctggag gtcctccagc aggccgctgt
     7381 tgctgtcggc gatgttcacc gcccccaggt ccttcagcat ggccccggca atgttgtcgc
     7441 tgtttttggc ccgcaccccg ctggaatagg cccgcagcag cagcacagtg gggtcctccc
     7501 cctccggcac ggaggcgatg gccgcgtcaa tgccgctctg gatgtccaac ccgtttttct
     7561 ggtacaaatc ggaccggcct gtcaaatcag tgcagatctt caacatattc aaataatcgt
     7621 caaaggtctc caccttgaac caggcggcgg ggatcccggc ggccgccagg ctgtccttca
     7681 gccccatctg tccgtccagg tccggggtga gcagcaccag gtccggctcg gcggcaagca
     7741 gggcctccag gtcgggctgc tggttggcgc ccagattcac ggtgtcctcc ggaagctcca
     7801 ggccccgctc gtcataggcg tcctgggtga ccgccgccac ctctcctccg gccagcagcc
     7861 aggtctcggc atagctgccc agcagggcgg ctacccgctt ggggggctgc tccagctcca
     7921 cggtattccc caggctgtcg gtaaagatac aggtcccctg ggcgctctgc tccacaccgg
     7981 cagaggatgt gggggcagag gcggaggcgt ctccctctcc gggggcgcag gcggtcagca
     8041 gcagcacggc cgccgccagg accgtaaaca gtcgtcgcat cgtcgatctt cctttcgcat
     8101 tgtgatctag gtagaaaagt ttacccgtta ttgtaccata tttgctcctg gcgcgccagt
     8161 ccggccctgt cttttttcca cctccgccaa agtttttccg ctgtcttgaa aattcccgcc
     8221 cttttcgtta caataaagcc gaatcgatcc caggactaac ctcgggaccg gaaagaagga
     8281 gcatcatgga acaacaacag tcctattccc cccgcaccct tcaggatctt gttcccggac
     8341 agagcggcat gatcctgtct gtgggcaacc agtcgggggc ggtcaagcgc cggctggtgg
     8401 atatgggtct gaccccgggg accacggtga aggtgaccaa ggtggccccc ctgggcgacc
     8461 c
//
LOCUS       DBMDSJ010000023         8364 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2642_length_8364_cov_1.937674, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000023 DBMDSJ010000000
VERSION     DBMDSJ010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2642_length_8364_cov_1.937674"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(18..248)
                     /locus_tag="ACZPAQ_01190"
     CDS             complement(18..248)
                     /locus_tag="ACZPAQ_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage-shock protein"
                     /protein_id="HIR0902619.1"
                     /translation="MITLFTLSLLCLAVILVLGVVLTILSVPFLVILGLLPWLLRIAA
                     AVLLIRGLLDHPFRWENLIPAAVAFGLSVLLH"
     gene            complement(352..1023)
                     /locus_tag="ACZPAQ_01195"
     CDS             complement(352..1023)
                     /locus_tag="ACZPAQ_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR0902620.1"
                     /translation="MKKIPTYSGTLRSHLLAVPHCISECSGIRIFGRRIKSLVFTTDV
                     AIIKNVNGDAIIAVYPFTPQPSIAQAIISVADVPVFVGVGGGMTSGSRSVRQAEYAEH
                     QGAFGVVVNAPITDETITQIKHVVDIPVIATIISSEMDVAGRVRAGADILNISGAART
                     PEIVAKIRKDFPDIPIIATGGPTEEDILRTIQAGANAITYTPPSTGELFSVRMHQYRA
                     QFHPE"
     gene            complement(1064..2368)
                     /locus_tag="ACZPAQ_01200"
     CDS             complement(1064..2368)
                     /locus_tag="ACZPAQ_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902621.1"
                     /translation="MNSVGIFTTTRSLQHIMDIDQEIRTFCNVTYLPYTTLEHLLYLY
                     EQNAEQFDALIFSGSYPYNVIRKRFPSVEEIPHSYFNISDRDYYQLIAHLAVQNPELD
                     FSRIYFDRPEIPVDFHSIFLRDGVPLLGTAPIDWSTVDAADWYQPLKLYYKEIWDSGK
                     VDLLVTRFGSMEDYFRQHNIRHRFLFPSPGSMLETFRGLVMQLRTQSMHDSAACLGLI
                     RSIMPLSKEQWAALGDRLQACNKQFGTPFLIYEHGEHYEITTNISVLKDLTQQYTICP
                     VTAFLEAGLDFPICTAWGCAGNVVDAHRNAQRALKEALLCKGSAAFIVMEDNTIIGPL
                     SSVRRISYTDAPDEKRLELSRRLSISPLYLSKIVSVLTQKGNDTLSAEELSFYLDITP
                     RSALRILSKLEAGGYASVQFRRQLNLRGRPAKIYQIHIQDLT"
     gene            2699..4135
                     /locus_tag="ACZPAQ_01205"
     CDS             2699..4135
                     /locus_tag="ACZPAQ_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR0902622.1"
                     /translation="MATKKERALEVIEEKKALLWPMSDTLWDHPETGFHERYAAELYG
                     KTLEQEGFQVERDLAGIPTAFSGTYGQGGPVIGFLGEFDALPGLSQAAGADEKRPVEE
                     NGPGHGCGHNLLGVGSLAAAIALKHYLQDSGLPGTVKFFGCPAEENGSGKGFMARDGV
                     FNGVDIAFSWHPGEVNSVSTESTMANYQISYRFQGVSAHAAMSPELGRSALDALELMN
                     VGVQFLREHVPTDTRIHYAITDTGGNAPGVVQSHAQALYLLRAVQLPQVKELYQRVNQ
                     IARGAAMMTGTEVEITFIKSCSNMLINTELNQLMQRNLVEVAPVQLEEEDLELARRIR
                     ETLGPVHSYFDTVAAEITDPEERARVAADADSLVHRIVLPLARERQGFVSSDVGDVSW
                     NCPVAQINAATMPAGVPMHSWQMVAVGKSAMAKKGMLYAAKVMAGSAIDALEDPEIIR
                     RAQEEFCQRTGGQKYASPIPPEVKPRID"
     gene            4227..5504
                     /locus_tag="ACZPAQ_01210"
     CDS             4227..5504
                     /locus_tag="ACZPAQ_01210"
                     /inference="COORDINATES: protein motif:HMM:NF015557.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR0902623.1"
                     /translation="MSPIVICIALFVVMLVLFFTNKIPMSFTALAAMVALVLFGCLDS
                     SAAIATFGSNTVITMASMFVVAAGLTRTQMINNVTRLLYKVTEGSFTKVLASYVVITC
                     IIGQFVPSIVAIFALVHPLVQNMCDRMNISPSKMMYPIAIAAVSTSFIIEPIGPYAAW
                     YVTQNGYMESYGWTSTQLNMWNETAVFLPVGIVTILLAIFVVPRFLPDQPERPITAIG
                     GRKLQEQAPLSPVREFLGYGVFVAVILGLMLGYTSWQVTLIGAIVLVASGVLTEREAI
                     ENMNMSTVLLYVGVSAFGTALAQTGAAEMLADVFSKMLDGITNGYIVGFTFYVVGFLM
                     TSLLYNRAVSTVLIPLVVITCSSIGCDPRGPIILVALSTMSSLVTPMATAVVPMAMNA
                     GGYSAKTCLKVGLIPGVVRGIVGTLIAMTLYPM"
     gene            complement(5599..>8364)
                     /locus_tag="ACZPAQ_01215"
     CDS             complement(5599..>8364)
                     /locus_tag="ACZPAQ_01215"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR0902624.1"
                     /translation="AQDWLLMFKNETHNHPTEIEPFGGAATCIGGCIRDPLSGRAYVY
                     QALRLTGCGDPRAPYDRTMEGKLPQRKLATTAAAGYSSYGNQIGLATGHVAEVYHDGY
                     VAKHLEVGAVVGAAPASNVVRERPAPGDVVVLLGGRTGRDGIGGATGSSKSHNKASLT
                     TMASEVQKGNAPEERKIQRLFRDSQVTRMIKRCNDFGAGGVSVAIGELAAGLRIDLDA
                     VRKKYDGLDGTELAISESQERMAVVVAPEDADRFIAAAQAENLEAYPVAVVTESPRMV
                     MTWKGQTIADLSRAFLDTNGADKHTSVTVPDRSAAQRQPSPRFRDLRDMASSLKCASR
                     RGLTERFDGSIGAASVLMPFGGSTQRTPAQSMAALLPVLPGQSTDLCSVMAWGFDPDT
                     MVRDPFTGAAASVVDSMAKLVAAGCDRRQAYLTFQEYFEKLRDEPLRWGKPFSALLGA
                     LDAQLGLDVAAIGGKDSMSGSFLDLDVPPTLISFAIAPAKAGEVLTPEFKGAGHPVYL
                     LPGFPSYGELKTAWDSFHQLCRQGKVKAAWAVEAGGVAEAVMKMSFGNSIGFQADVDA
                     AAPWYGPCSGAIVFELEGEGFLPGMAWRIGTTTQEPVITIGEDTASVAELLERNEGVL
                     EQVYPTRAGKTEKVEAISCGERAPIVCQSRTARPRAVIPVFPGTNCEYDTAQACLRAG
                     IQPEIVVVRNLSTDLLAQSAQALEEAIRRSQMVVLPGGFSGGDEPDGSGKFIASFLRN
                     PRLTDAIHDLLRDRDGLMLGICNGFQALIKLGLVPYGEIRPMDDQCPTLTYNLIGRHQ
                     SRYVTTRVASVRSPWMLKSQVGDLHAIPISHGEGRFVASDEMVRRLIQNGQVATQYVD
                     GAGVPSLDIDVNPNGSIMSIEGIFSPDGRVFGKMGHSERYGDFVGRNIPGDKHQPLFE
                     SGAEYFK"
BASE COUNT         1668 a         2381 c         2730 g         1585 t
ORIGIN      
        1 cgcccctgct ctttaggtca gtgcagaaga acagaaagcc caaaggcgac agcggcaggg
       61 atcagatttt cccaacggaa gggatggtcc agcagtccgc ggatcagcag gaccgcggcg
      121 gcgatgcgca gaagccaggg cagcaggccc aggatgacca ggaacggcac actgaggatg
      181 gtcagcacca cgcccaggac caggatcaca gccaggcaga gcagagacag cgtgaatagg
      241 gtaatcattt tgaatttcct ccttcctact gacgctatcc taccatttga ttctgaaagg
      301 gggagaaagc cagcattaaa aacagcttaa aagcggcggc ggtcatacct tttattccgg
      361 atggaactgg gcgcggtact ggtgcatccg cacggaaaac agctcaccgg tggagggagg
      421 ggtataagta atggcgttgg ctccggcctg gatggtgcgg aggatgtcct cctctgtggg
      481 gcccccggtg gcaatgatgg ggatgtctgg gaaatctttt cgaatcttgg ccacgatctc
      541 aggggtgcgt gcggcccctg agatattcag aatatcggct ccggcccgga cacgcccggc
      601 gacatccatc tcagaagaga tgatggtggc gatgaccggg atgtccacca catgcttgat
      661 ctgggtgatg gtctcgtctg tgatgggggc gttgaccaca acgccgaagg ccccctggtg
      721 ctctgcgtac tccgcctgcc ggacggagcg ggagccgctg gtcatcccgc ctcccacgcc
      781 cacgaacacc gggacatcgg ccacactgat gatggcctgg gcaatggagg gctggggggt
      841 aaagggatag accgcgatga tggcgtctcc gttgacgttc ttgatgatgg ctacatcggt
      901 ggtgaacacc agggatttga tgcggcggcc aaagatgcgg atgccgctgc attctgaaat
      961 acagtggggg acggccagca aatggctgcg cagagtcccg ctgtatgtag ggatcttttt
     1021 cataggcgta gtacctcctg gggatgagac aggtttgggg gcgtcaggtc aggtcctgga
     1081 tgtggatctg atagatcttg gccgggcggc ctctcaagtt gagctgccgc cggaactgta
     1141 cagaggcgta gccgccggcc tccagcttgg ataggatccg gagagcactg cgaggggtga
     1201 tatccagata gaaggatagc tcctccgcag agagagtgtc gtttcctttc tgggtaagga
     1261 cggagacgat cttactcaaa tagagggggg agatggagag acggcggctg agctccagcc
     1321 gcttttcatc gggcgcatcg gtataggaaa tgcggcggac gctggagagc gggccgatga
     1381 tggtgttgtc ctccatcacg ataaaggcgg cggacccctt gcacagcagc gcctctttca
     1441 gcgcccgctg ggcgtttcgg tgggcatcca ctacgttgcc ggcacagccc caggcggtac
     1501 agatgggaaa atccagtccc gcctccagaa aggcagtcac aggacagatg gtgtactgct
     1561 gcgtgaggtc tttcaggact gaaatattgg tggtgatctc atagtgctcc ccgtgctcat
     1621 agatgaggaa gggggtgccg aattgtttgt tgcaggcctg aagacggtcc cccagtgcgg
     1681 cccactgttc cttggacagg ggcatgatgg aacggatcag ccccaggcag gcggcggaat
     1741 catgcataga ctgggtgcgc agctgcatga ccagaccgcg gaaggtctcc agcatagaac
     1801 cgggagaggg gaaaaggaac ctgtggcgga tattgtgctg acggaagtag tcctccatac
     1861 tgccgaaacg ggtcaccagc aggtccacct ttccgctgtc ccaaatctct ttgtagtaca
     1921 gtttgagagg ctgataccag tccgcggcgt ccactgtgct ccagtcgatg ggggcggtgc
     1981 ccaagagagg gacaccgtcc ctgagaaaga tagagtggaa atctactggg atctccggcc
     2041 ggtcgaagta aatgcgtgag aaatcaagct ccggattttg gaccgccagg tgggcgatca
     2101 gctgatagta gtctcggtca gagatattga agtaggaatg ggggatctct tccacactgg
     2161 gaaatcgctt tcggatgaca ttataagggt aggaaccgct gaatatcagg gcatcgaact
     2221 gctccgcgtt ttgctcatag agatagagca ggtgctccag agtggtatag ggcaggtagg
     2281 tgacattgca gaaagtgcgg atctcctgat cgatgtccat gatgtgctgg agggagcgtg
     2341 tggtggtaaa aatgccgaca gagttcattg ggaccgatca cctcagctca tattatatcg
     2401 gaaccgctcc gcgcagacaa gcgtatgacg caaaataaga cgaaaatctt ttctgcgtgt
     2461 cgtcaaatca agatgtccaa aagaacggcc agatatgcaa gataaagctg gtgatctgac
     2521 taaatcaaga acaaaatatt tgtaataaac ggggaaatta aaaaatgctg tccttttcta
     2581 ttgacagtct gggcatggaa atagtatagt tacaccataa tttccgaaac atgtctgtaa
     2641 ttatatattt aatttccaat aattccgaga caggagcagt aggaaggaga acgagtaaat
     2701 ggcgacaaaa aaggagcggg cgctggaggt cattgaggaa aaaaaggcgc tgctttggcc
     2761 catgagcgac acgctgtggg accacccgga gaccgggttc cacgagcgct atgccgctga
     2821 gctctatggc aagacgctgg agcaggaggg ctttcaggtg gagcgggatc tggccggtat
     2881 ccccaccgcc ttttccggga cctatggcca gggcgggccg gtgatcggat ttttgggaga
     2941 atttgacgcg ctgcccggtc tcagccaggc ggccggagcg gacgaaaagc ggccggtgga
     3001 agagaacggc cccggacacg ggtgcgggca caatctgctg ggggtcggct cgctggccgc
     3061 cgccatcgcc ctgaagcact acctgcagga ctccggcctc cccggcaccg tcaaattctt
     3121 cggatgcccg gcggaagaaa acggatccgg gaaaggattc atggcgcggg atggcgtgtt
     3181 taatggagtg gacattgctt tcagctggca tcccggggaa gtaaatagcg tctctacgga
     3241 gagtaccatg gccaactacc agatcagcta ccgcttccag ggagtgtcgg cccacgcagc
     3301 catgtctccc gagctgggaa ggagcgcctt ggacgccctg gagctgatga atgtgggagt
     3361 gcagttcctg cgagaacacg tccccacaga cacccgcatc cactatgcca tcactgatac
     3421 agggggcaat gcccctggtg tagtgcagtc ccacgcccag gcactgtatc tgctgagagc
     3481 agtccagctg cctcaggtaa aggagctcta ccagcgggtc aatcaaattg ccagaggagc
     3541 ggcaatgatg acagggacag aggtggagat cacatttatc aaatcatgct ccaacatgct
     3601 cattaacacg gagctcaacc agctgatgca gcgaaacttg gtggaggtcg cccctgtcca
     3661 gctggaggag gaggacctgg aactggcccg gcgcattcgg gagacattgg gcccagtaca
     3721 ctcttatttc gatactgtgg ccgctgagat taccgacccg gaggagcgtg ccagagtggc
     3781 ggcggatgcc gattccctgg tccataggat cgtgctcccg ctggccagag aacgtcaggg
     3841 ctttgtctcc tccgatgtgg gcgatgtaag ctggaactgc ccggtagcgc agatcaatgc
     3901 cgccaccatg ccagcaggag tgcccatgca ctcctggcag atggtagctg tagggaaatc
     3961 tgctatggca aaaaagggga tgctttatgc cgccaaggtc atggccgggt cggccattga
     4021 cgcgctggag gaccccgaga tcatacgccg ggcccaggag gaattctgcc aacgcacagg
     4081 tggtcagaaa tacgcgtccc ccataccgcc ggaggtaaag cccaggatcg actgaacggc
     4141 tctggccggc gcacggaaaa gtggcccgct tggtagggag gggcgggcat agcctgagac
     4201 ggagaaaagc agaggaggaa aaaagaatgt cacctattgt aatctgtatc gcgctgtttg
     4261 tggtgatgct tgttctgttc ttcacgaaca agatccccat gtcctttacc gcgctggcgg
     4321 ccatggtggc cctggtgctc ttcggatgcc tggacagcag tgcggccatt gccacatttg
     4381 gcagcaacac ggtcatcacc atggcctcca tgttcgtagt ggccgcggga ctgacccgga
     4441 cccagatgat caataatgtc accaggctgc tgtacaaggt cacggagggc tccttcacca
     4501 aggtactggc gtcctatgtg gtgatcacct gcatcattgg acagttcgtg cccagtatcg
     4561 tggccatctt cgcgctggtc cacccgctgg tccagaacat gtgcgacaga atgaacatca
     4621 gtccctccaa aatgatgtac cccattgcca tcgcggccgt ttccaccagc ttcatcatcg
     4681 agcccatcgg cccctatgcc gcctggtatg tgacgcagaa cgggtacatg gagtcctatg
     4741 ggtggacctc tacccagctg aacatgtgga acgagacggc ggtcttcctg ccggtgggga
     4801 tcgtcactat cctgctggcc atttttgttg ttcctcgctt cctccccgat cagccggagc
     4861 ggcccattac ggccatcggc gggcgcaagc tccaggagca ggctcccctc agtccggtgc
     4921 gggagttcct gggctacggt gtgttcgtgg ccgtcattct cggcctgatg ctgggctata
     4981 cctcctggca ggtgacgctg atcggcgcca tcgttctggt ggcctccggg gtactgacag
     5041 agcgggaagc cattgaaaac atgaacatga gcaccgtcct gctatatgtg ggcgtgtccg
     5101 catttggaac ggctctggcc cagaccggtg ccgccgaaat gctggccgat gtgttctcca
     5161 agatgctgga tgggattacc aacggctata ttgtcggctt cactttctat gtggtgggct
     5221 tcctgatgac ctccctgctg tacaaccggg ccgtgagcac ggtgctgatc cccctggtgg
     5281 tcattacatg cagttctatc ggctgtgacc cccgcggtcc catcatcctg gtggcgctgt
     5341 ccaccatgtc ctctctggtc acccccatgg ccactgctgt ggtccctatg gcgatgaatg
     5401 ctggcggata cagcgctaag acctgtctga aagtcggact cattcccggc gtggtccgcg
     5461 ggatcgtggg caccctgatc gctatgaccc tgtaccccat gtaaactggt gttaaatgga
     5521 accgcgcgcc gacagctccc tggatacaaa acactccccc gaaggcttac tgcgttcggg
     5581 ggagtgtttt ttgacatttt acttgaaata ttccgcgccg gactcgaaca gcggctggtg
     5641 cttatctccg ggaatgttcc ggcccacaaa gtcgccgtac cgctccgagt gccccatctt
     5701 gccgaacacc cggccgtccg gggagaagat gccctcgatg gacatgatgg agccgttggg
     5761 gttgacgtcg atgtccaggg aggggacgcc cgccccgtcc acatactggg tggccacctg
     5821 gccgttctgg atcagacggc ggaccatctc gtcggaggcc acaaagcggc cctcgccgtg
     5881 ggagatgggg atggcgtgca ggtcgcccac ctgggacttc agcatccagg gggaacggac
     5941 cgaggccacc cgggtggtga cgtaccggct ctggtgccgg ccgatcaggt tgtaggtcag
     6001 agtggggcac tgatcgtcca tggggcggat ctccccgtat gggaccagcc ccagcttgat
     6061 gagggcctgg aagccgttgc agatgcccag catcaggccg tcccggtccc gaagcaggtc
     6121 atggatcgca tcggtcagcc gggggttgcg caggaaggag gcgatgaact ttccggagcc
     6181 gtcgggctcg tcgccgccgg agaagccgcc gggcaggacc accatctggc tgcggcggat
     6241 ggcctcttca agggcctgag cggactgggc cagcaggtcg gtggacaggt tgcggaccac
     6301 cacgatctcg ggctggatgc ccgcccgcag gcatgcctgg gcggtgtcat attcgcagtt
     6361 ggtgccgggg aagacaggga tgaccgcccg gggccgggcc gtccggctct ggcagacgat
     6421 gggggctcgc tccccgcagg agatggcctc cactttttcc gtctttcccg cccgggtggg
     6481 atacacctgc tccagcaccc cttcgttccg ctccagcagc tcggcaacgg aggcggtgtc
     6541 ctccccgatg gtaatgactg gctcctgggt ggtggtgccg atgcgccagg ccatgccggg
     6601 gaggaacccc tccccttcca gctcaaacac gatggcgccg gagcaggggc cgtaccaggg
     6661 agcggcggca tccacatcgg cctggaagcc gatggagttg ccgaaggaca tcttcatcac
     6721 cgcctcggcc acgccgccgg cctccaccgc ccaggcggcc ttcaccttgc cctggcggca
     6781 caactggtgg aaggagtccc aggcagtctt cagctcccca taggagggga agccgggcag
     6841 cagatacacc gggtgccccg cccccttgaa ttcgggcgtg agcacttccc cggccttggc
     6901 gggggcgatg gcgaaggaga tcagcgtggg gggcacgtcc agatccagga aggagccaga
     6961 catggaatcc ttgccgccga tggcagccac atccagcccc agctgggcgt ccagggcccc
     7021 cagcagggca gagaagggct tgccccagcg caggggctcg tcccgaagct tctcaaaata
     7081 ctcctggaaa gtgaggtagg cctggcggcg gtcacagccg gcggccacca gcttggccat
     7141 agagtccacc actgaggcag ccgcaccggt gaaggggtcg cggaccatgg tgtctgggtc
     7201 gaagccccag gccatcacgg agcacaggtc ggtgctctgg ccgggcagca cgggcagcag
     7261 ggcggccata gactgggccg gggtgcgctg ggtgctcccg ccgaagggca tgagcacgga
     7321 ggcggcgccg atggagccgt caaaccgctc ggtcagtccc cggcgggagg cgcacttcag
     7381 gctggaggcc atgtcccgca ggtcccggaa ccggggggag ggctgccgct gggcggcgga
     7441 gcggtcgggt acggtgacag aggtgtgctt gtccgccccg ttggtgtcca ggaaagcccg
     7501 ggacaggtcg gcgatggtct ggcccttcca ggtcatcacc atcctggggc tctccgtgac
     7561 caccgccacg gggtatgcct ccaggttttc cgcctgagcg gcggcgatga agcggtccgc
     7621 gtcctccggg gccaccacca ctgccatgcg ctcctggctc tcagagatgg ccagttcggt
     7681 gccgtccagg ccgtcatact tcttgcgtac cgcgtccaag tcgatgcgca ggccggcggc
     7741 cagctcgccg atggccacgg agacgccgcc ggcgccgaag tcgttgcacc gcttgatcat
     7801 gcgggtgacc tggctgtccc ggaagaggcg ctggatcttc cgctcctcgg gggcgttgcc
     7861 cttctggacc tcgctggcca tggtggtgag ggaagccttg ttgtggctct tggaggagcc
     7921 ggtggctccg ccgatgccgt cccggccggt gcggccgccc agcaggacga ccacgtcccc
     7981 gggggcagga cgctcccgca ccacgttgga ggccggcgcg gcgcccacca cggcgcccac
     8041 ctccaggtgc ttggccacat agccgtcgtg gtacacctcg gccacatggc cggtggccag
     8101 gccgatctgg ttgccgtagg aagagtagcc ggcggcggcg gtggtggcca gcttgcgctg
     8161 gggcagcttg ccctccatgg tccggtcata gggggcgcgg gggtcgccgc acccggtcag
     8221 gcgcagagcc tgatacacat aggcccggcc ggaaagcggg tcccggatgc agccgccgat
     8281 gcaggtggcc gcgccgccga agggttcgat ctcggtgggg tggttgtggg tctcgttctt
     8341 gaacatgagc aaccagtcct gcgc
//
LOCUS       DBMDSJ010000024         7938 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2805_length_7938_cov_1.840373, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000024 DBMDSJ010000000
VERSION     DBMDSJ010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_7938_cov_1.840373"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="ACZPAQ_01220"
     CDS             <1..1080
                     /locus_tag="ACZPAQ_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR0902625.1"
                     /translation="TSETSFLGPVRNPWDRERVPGGSSGGAAAAVAARSSWYAIGSDT
                     GGSIRQPAAFCGVTGIKPTYGTVSRYGLIAYASSLDQMGPLARDAADCAAVLDLVMGR
                     DPRDGTSLDVPAGGLLAALTGDVRGLRIGLPRECFGEGLDSAVRACVLAAADVLRDRG
                     AELVPLSFPMMEYVIPTYYIIACAEASSNLSRFDGVKYGWRAEGYGDLRELYQQTRTE
                     GFGPEVRWRILLGTFVLSSGYYDAYYLKALRAKGMIKRAFDEAFCQCDVMLTPTAPAA
                     APRLGESLSDPLRMYLSDIDTVPVNLAGLPGLSMPCGFDETGLPVGAQLIGPPLGEKN
                     VLNAAHAFQMETDWHTRAPAEKEVG"
     gene            1082..2530
                     /gene="gatB"
                     /locus_tag="ACZPAQ_01225"
     CDS             1082..2530
                     /gene="gatB"
                     /locus_tag="ACZPAQ_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR0902626.1"
                     /translation="MTWETVIGLETHAELATRTKIFCSCTTRFGGEPNTHCCPICTGM
                     PGTLPMVNRKVVEYACRAGLALNCEITRRSRFDRKNYFYPDLPKAYQVSQLYLPIARN
                     GRVSIATAAGEKVVRIHELHMEEDAGKLVHDPWLDQTRADYNRCGVPLVEIVTEPDFR
                     SAEEVIAYLEKLKSILEYLGISDCKMQEGSLRCDVNLSVRPAGSRELGARTEMKNLNS
                     FKAIARAIAYEARRQAELLEEGGRVVQETRRWDDNKDATFSMRSKEDAQDYRYFPEPD
                     IPPLELSEEYLEGLRAERPEMAEERKQRYQTQLGLPEYDAGIITGQKALVDFFEEVTA
                     LGVPAKEAANWMLGDMMRRLKEEELEPKDMRLTPATLVKLIGMVQAGRINRNTASKVF
                     RGIFAEDLDPEEWVTVHGLEQIRDERVIAGAVARVLEEHPQSAADYRAGKEKALGFLM
                     GRVMKELKGKADPGAVNALLKSRLETGGSEGG"
     gene            2840..3256
                     /gene="mraZ"
                     /locus_tag="ACZPAQ_01230"
     CDS             2840..3256
                     /gene="mraZ"
                     /locus_tag="ACZPAQ_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR0902627.1"
                     /translation="MTGTYEHSIDAKGRLFIPAKLREELGVTFYLAMGVDECLAIYPQ
                     ETWNRFTEKFASLPMSQSAAMRPLFANASKCELDSQGRIVIPQKLRKYAGLEKDAVII
                     GVNDRAEIWSAETWNAREEEEMTPEKMKACLAALGF"
     gene            3261..4199
                     /gene="rsmH"
                     /locus_tag="ACZPAQ_01235"
     CDS             3261..4199
                     /gene="rsmH"
                     /locus_tag="ACZPAQ_01235"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR0902628.1"
                     /translation="MSETFTHRPVLLNECIQALNIRPEGVYLDGTLGRAGHSREIARR
                     LTTGRLICVDRDQAALEAAEERLAQWRDRVTLVHSNFDQIDRILNELSLSGADGMLFD
                     LGVSSPQLDDSTRGFSYMADAPLDMRMDQSGGMTAAEVVNQWPQEELRRILYQYGEER
                     YAPQIAAAIVRRRQDRPIQTTMELAQIVREAMPARALKEKQHPAKRSFQAIRIAVNDE
                     LASVDRMLQAAVPRLNPGGRLAVITFHSLEDRIVKTGMAEFARGCTCPPDFPVCVCGK
                     KPQIRLVSRKPILPSEEEVRDNPRARSAKLRAAEKL"
     gene            4269..4823
                     /locus_tag="ACZPAQ_01240"
     CDS             4269..4823
                     /locus_tag="ACZPAQ_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902629.1"
                     /translation="MATRRRSQLSSYRTYGSVAYAPAYDGTAVRAPRREEVLRPKPRV
                     RPRERVLTRTQVQVREAGQASVFAVIGFLAVGVFAALLLLSYTQYAVLTDQVVTLRSE
                     LTTLQTENATLSAEYEKVFDMERIQAAVGDTMIRPTGDQVEYIDLSEPDSVVVYGQED
                     AKTGIPGLLAGLEEIISSLIEYFR"
     gene            5003..7441
                     /locus_tag="ACZPAQ_01245"
     CDS             5003..7441
                     /locus_tag="ACZPAQ_01245"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR0902630.1"
                     /translation="MDDRRERNTGRSGRESGSKRKIFRRTVFLMLVCGVGLFIPLIGQ
                     LWNIAIVHHEEYQAEAVEQQTRDVEVAAFRGSIYDSSGDVLAMSATVYNLILSPRDLE
                     ASVDESKYKDEEGNADLTAYQAALQAKKDLVLDGVLAVLDLDREALADRLERTYSQYE
                     VLKTNIEKEQAEQLRAFITENKLSHFLYLTPTTKRYYPYGDLAAHVLGFCNSEGGAYG
                     VEAIYNDVLEGTVGRVVTTRTGTGVEMYDSYSEYVDAEDGCDLTLTIDTTIQSYAERI
                     LEEGIKAYDVQRGGWCVVLDPQTGAVLAMASSPDYDPNQYGTIRDESLRAQVEADVQA
                     ELEKRQGEKAEGKTDEEIYQEAVNAATGTAQNKQWRSQAVNDTYEPGSTFKALVLAAA
                     LEEGVVSESDTFYCPGYYMVEGWPKPISCSKKAPGHGTQTLAEAVQNSCNPAFMQIGQ
                     RLGIEKFYEYFQAFGLTEPTNIDLNGEGVSTVWPLNTMTGVDLAVGSFGQRFTVTPIQ
                     MITAFAATINGGKLLQPYVVQTVTAADGTVVENTEPTVVRQVVSQETSDKCREILTSV
                     VMAEKGTGKNARQAGYTIGGKTGTSETTVPGEVVVSFMGFAPADDPQILVLLAYNTPA
                     RSAEGSSIGTTGTWISGGNMGAPMAGKLIGEILDYLGVEKEYTADEAALADLQTPKVT
                     GMTPADAEAALEKKGLDSRTVGEGETVTAQVPAAGSVIPGESTVILYLGGAEPEETGT
                     VPDVTGMSFAAARSKLESAGFFMRAAGAATYLGSSSKAQGQSVAGGETAAIGTIVDVT
                     FSTPQVEDGYLYDG"
     gene            7539..>7938
                     /locus_tag="ACZPAQ_01250"
     CDS             7539..>7938
                     /locus_tag="ACZPAQ_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR0902631.1"
                     /translation="MKLRDLLAGVPLTGGNADLNMEINSISYDSRTLEPGALFVTLSG
                     SKTDGHRYVAQALEKGAAAILCREPPRTGSWLSTPDPRAALARVSAAWFGYPGEEMTL
                     IGVTGTNGKTTTTYLIKAMLEGALKTRVGLI"
BASE COUNT         1561 a         2415 c         2719 g         1243 t
ORIGIN      
        1 accagcgaga cctctttcct ggggccggtg cgcaacccct gggaccggga gcgggttccc
       61 ggcggctcct ccggcggtgc cgcggcggcg gtggccgccc gctccagctg gtacgccatc
      121 gggtcggaca ccggcggctc catccgccag ccagccgcat tctgcggcgt caccgggatc
      181 aagcccacct acgggaccgt gtcccggtac gggctgatcg cctacgcctc ctccctggac
      241 cagatgggtc ccctggcccg ggacgcggcc gactgtgctg ccgtgctgga cctggtgatg
      301 ggccgcgacc cgagggacgg cacctctctg gatgtgcctg ctggggggct gctggccgcc
      361 ctgaccggag acgtgagggg cctgcggatc gggctgcccc gggagtgctt cggggagggg
      421 ctggacagtg cggtgcgggc ctgcgtcctg gcggcggcgg acgtgctgcg ggaccggggg
      481 gcagagctgg tgcccctgtc cttccccatg atggagtatg tcatcccgac ctattatatc
      541 attgcctgcg cggaggcgtc ctcaaacctg tcacggttcg acggggtgaa gtatggctgg
      601 cgggcggagg ggtacgggga cctgcgggag ctgtatcagc agacccgcac cgagggcttc
      661 ggccccgagg tccggtggcg gatcctgctg ggcaccttcg tgctctcctc cggctactac
      721 gacgcctact acctgaaggc tctgcgggca aaggggatga tcaagagggc cttcgacgag
      781 gcattctgtc agtgtgatgt gatgctcact cccaccgctc ctgccgcggc cccccggctg
      841 ggggagagcc tgtccgaccc cttgcggatg tacctgagcg acatcgacac cgtgccggtg
      901 aacctggcgg ggctgccggg gctgtctatg ccctgcggct ttgacgagac ggggctgccg
      961 gtgggcgccc agctcatcgg ccctccgctg ggggagaaga acgtgctgaa cgccgcccac
     1021 gccttccaga tggagacaga ctggcatacg cgggctccgg cggaaaagga ggttggatga
     1081 catgacctgg gaaactgtga tcgggctgga gacccacgcg gagctggcca cccgcaccaa
     1141 gatattctgc tcctgcacca cccggttcgg gggagagccc aacacccact gctgccccat
     1201 ctgcactgga atgccgggga ccctgcccat ggtgaaccga aaggtggtgg agtacgcctg
     1261 ccgtgccggg ctggcgctga actgcgagat cacccggcgc agccggttcg accggaaaaa
     1321 ctacttctat cccgacctgc ccaaggcgta ccaagtctcc cagctctacc tccccattgc
     1381 ccggaacggg cgtgtgagca tcgccacggc ggcgggggag aaggtggtcc gcatccacga
     1441 gctgcacatg gaggaggacg cgggcaagct ggtccacgac ccctggctgg accagacccg
     1501 ggcggactac aaccgctgcg gcgtgccgct ggtcgagatc gtcaccgagc ccgacttccg
     1561 ctccgctgag gaggtgatcg cctatctgga gaagctgaag tccatcctgg agtatctggg
     1621 gatctcggac tgcaagatgc aggagggctc cctgcgctgt gacgtgaacc tctccgtccg
     1681 gccggcgggc tcccgggagc ttggggcccg gacggagatg aagaacctga actccttcaa
     1741 ggccattgcc cgggccatcg cctacgaggc ccgccgccag gcggagctgc tggaggaggg
     1801 cgggcgcgtg gtccaggaga cccggcgctg ggacgacaac aaggacgcca ccttctccat
     1861 gcgctccaag gaggacgccc aggactaccg ctacttcccg gaaccggaca tcccgcctct
     1921 ggagctgtcg gaggagtacc tggaggggct gcgggcggag cggcccgaga tggcggagga
     1981 gcggaagcag cggtatcaga cccagctggg cctgccggag tacgacgccg gcatcatcac
     2041 cgggcagaag gccctggtgg acttcttcga ggaggtgacg gccctggggg tgccggccaa
     2101 ggaggcggcc aactggatgc tgggggacat gatgcgccgc ctgaaggagg aggagctgga
     2161 gccaaaggac atgaggctga cccccgccac cctggtcaag ctgatcggga tggtccaagc
     2221 gggacggatc aaccgaaaca ccgcatccaa ggtgttccgg ggcattttcg cggaggacct
     2281 ggacccggag gagtgggtga cggtccacgg cctggagcag atccgggacg aaagggtcat
     2341 tgccggcgcg gtggcccggg tcctggagga gcatccccag tctgcggcgg attatcgggc
     2401 gggcaaggag aaggcccttg gtttcctcat gggccgggtg atgaaggagc tgaagggcaa
     2461 ggcggacccg ggggcggtaa acgctctgct gaagagccgc ctggagacag gaggatcgga
     2521 ggggggataa aagagtgccc cggaaaggaa agaaagcccg gtgtatcctg aacggagggg
     2581 cggtgccaca tattgtgcgc tgaaaaccgg tctatacaac gccttgcatg ggtcaaaaga
     2641 tgtaaaccaa aatcgggagg cggaaacaca gtttccgtgc cgaaaaaccg ctgattttct
     2701 gaaaaatcag cggttttttt ctgtgccgaa ttgaaaaaaa gtcttgcatt ttatgtaaag
     2761 tgcttttata ctaaaaggca gagtggggca aagtggagta aaatcccgct aagatccatc
     2821 aaagtgggga gggagagcga tgaccggcac ctatgaacac agcatcgatg ccaagggccg
     2881 tctctttatc cctgccaagc tccgtgagga gctgggcgtg accttctacc tggccatggg
     2941 ggtggacgag tgtctggcca tctaccccca ggagacctgg aaccgcttta cggaaaaatt
     3001 tgcctccctg cccatgagcc agtcggccgc catgcgcccc ctgtttgcta acgcctcgaa
     3061 gtgcgagctg gacagccagg gccgcatcgt catcccccag aagctgagaa aatacgcggg
     3121 cttggagaag gacgccgtca tcatcggcgt caacgaccgg gccgagatat ggagcgctga
     3181 gacttggaat gcccgggaag aggaggagat gaccccggag aagatgaagg cctgtctggc
     3241 cgcactgggg ttctgacgaa atgagtgaga cctttaccca ccgcccggtg ctgctgaatg
     3301 agtgcatcca ggcgctgaac atccgcccgg agggcgtcta tctggacggg accctgggcc
     3361 gggcaggaca cagccgggag atcgcccggc ggctgaccac cggacggctg atctgcgtgg
     3421 accgggacca ggccgccctg gaggcagcgg aggagcggct ggcccaatgg cgggaccggg
     3481 tgaccctggt ccacagcaat ttcgaccaga tcgataggat cttgaacgag ctgtccctgt
     3541 ccggcgcgga tgggatgctc tttgacctgg gggtgtcctc gccccagctg gatgacagca
     3601 cgagaggatt ctcctacatg gcggacgcgc ccctggacat gcggatggac cagagcggcg
     3661 ggatgaccgc cgcagaggtg gtcaaccagt ggccgcagga ggagctgcgg cgcatcctgt
     3721 accagtatgg agaggagcgg tatgcccccc agatcgccgc cgctatcgtc cggcgtcggc
     3781 aggacaggcc catccagacc accatggagc tggcgcagat cgtccgggag gccatgcctg
     3841 cccgggcact gaaagagaag cagcacccgg ccaagcggtc tttccaggcc atccgcatcg
     3901 cggtcaacga cgagctcgcc tcggtggacc ggatgctcca ggcggctgtg ccccggctga
     3961 atccgggggg acggctggcc gttatcacct tccactccct ggaggatcgg atcgtcaaga
     4021 ccggcatggc ggagtttgcc cggggctgta cctgcccgcc cgattttccg gtatgcgtat
     4081 gcgggaaaaa accccagatc cggttggtga gccgcaagcc gatcctgccc tcggaggagg
     4141 aggtccggga caatccccgg gcccgcagcg ccaagctccg ggcagcggaa aagctgtaaa
     4201 aaacgactct gcggtcgtct gtgatatgtg tgttataaag gatactgaag agaaatggag
     4261 tgaatgccat ggccacccgc cggcgcagtc agctaagctc ataccgaacc tatggcagtg
     4321 tggcctacgc ccccgcctat gacggcaccg ctgtccgggc gccgaggcgg gaggaggtgc
     4381 tccgtcccaa gccccgggtc cgccccaggg agcgggtcct cacccgtacc caggtccagg
     4441 tccgagaggc ggggcaggcc tcggtgttcg cggtgatcgg ctttttggcg gtgggggtgt
     4501 tcgccgccct gctgctgctg agctacaccc agtacgccgt gctgactgac caggtggtga
     4561 ccctgcgcag tgaactgacc accctccaga cggagaacgc tacgctcagc gcagagtatg
     4621 agaaggtatt tgacatggag cggatccagg cggctgtggg agataccatg atccggccca
     4681 ccggtgacca ggtggagtat atcgatctgt ccgagcctga cagcgtggtg gtgtacgggc
     4741 aggaggacgc caagaccggc atcccgggcc tgctggcggg gctggaggag atcatctcca
     4801 gtctgatcga atatttccgg tgacccgccg catatagtga tccatgggtg gacgaaagag
     4861 atagaccggg cggaaggggc tccccccgcg cccggagcaa gggggcgcaa gaaaagagag
     4921 cttttcttgc gcctcagatt atcaaggcag agcggaacgc acggcaaagc agggaaacgg
     4981 acccagagga ggagacccca ggatggacga tcgcagagaa cgaaataccg gccgcagtgg
     5041 acgggagagc ggctccaagc gcaagatatt ccgtcgcacg gtctttctga tgctggtgtg
     5101 cggcgtgggt ctgttcatcc cgctgatcgg ccagctgtgg aacatcgcca tcgtccacca
     5161 tgaggagtat caggctgagg cggtggagca gcagacacgg gatgtggagg tggctgcttt
     5221 ccgggggagc atctacgaca gcagcggaga cgtgctggcc atgtcagcca ccgtctacaa
     5281 cctgatcctg tctcccaggg acctggaggc cagcgtggac gagtccaaat acaaggatga
     5341 ggagggcaat gcggacctga cagcctatca ggcggccctc caggccaaga aggacctggt
     5401 gctggacggc gtgctggccg tgctggacct ggaccgggag gctctggcgg accgattgga
     5461 gcggacctac tcccagtatg aggtcctgaa gaccaacatc gagaaggagc aggcggaaca
     5521 gctgcgcgcc tttatcacgg aaaacaagct gtctcatttc ctgtacctca cccccaccac
     5581 caagcggtat tacccctacg gcgacttggc ggcccatgtg ctgggcttct gcaacagcga
     5641 gggaggcgcc tacggtgtag aggccatcta caacgatgtg ctggagggca cggtgggccg
     5701 ggtggtcacc acccgcaccg gcaccggcgt ggagatgtac gacagttact ccgagtatgt
     5761 ggacgcagag gacggctgcg atctgaccct gaccatcgac accaccatcc agtcctatgc
     5821 cgagcgcatc ctggaggagg ggatcaaggc ctatgacgtc cagcggggcg gctggtgcgt
     5881 cgtgctggac ccccagaccg gggctgtgct ggccatggcc agctccccgg actacgaccc
     5941 caaccagtac gggaccatcc gggacgagag tctgcgggcc caggtggagg ccgatgtgca
     6001 ggccgagctg gagaagcggc agggcgaaaa ggcggagggc aagaccgacg aggagatcta
     6061 ccaggaggcg gtgaacgccg ccacaggcac ggcccagaac aagcagtgga ggagccaggc
     6121 ggtgaacgac acctacgagc cgggctccac cttcaaggcc ctggtgctgg ccgcagcact
     6181 ggaggaaggc gtggtgtcag agagcgatac cttctactgc cccggttatt acatggtgga
     6241 gggctggcca aagcctatca gctgctccaa aaaggcgccg ggccacggca cccagaccct
     6301 ggcggaggcg gtgcagaact cctgcaaccc ggcctttatg cagatcggcc agcggctggg
     6361 catcgagaag ttttatgaat atttccaagc cttcggcctg acagagccca ccaatatcga
     6421 tctgaacggg gagggcgtga gtacggtctg gcccctgaac acgatgaccg gcgtggacct
     6481 ggcggtaggc tccttcggcc agcgattcac cgtcaccccc atccagatga tcacagcctt
     6541 cgccgctacc atcaacggcg gcaagctgct ccagccctat gtggtccaaa cggtgacggc
     6601 cgccgacggc acggtggtgg agaacacgga gcccaccgtg gtgcggcagg tggtcagcca
     6661 ggagaccagc gacaagtgcc gggagatcct caccagcgtg gtgatggccg agaagggcac
     6721 cggcaagaat gcccgccagg cgggctacac catcggcggc aagacgggca cctcggagac
     6781 caccgtcccc ggcgaggtgg tggtgtcctt catgggcttc gcccccgccg acgaccccca
     6841 gatcctggtc ctgctggcct acaacacccc agcccggtcc gcggagggga gcagcatcgg
     6901 caccaccggc acctggatca gcggcggcaa catgggtgcc cccatggcgg gcaagctgat
     6961 cggcgagatc ctggactatc tgggagtgga gaaggagtac accgccgacg aggcggccct
     7021 ggccgacctc cagactccca aggtcaccgg gatgaccccc gccgacgcgg aggctgccct
     7081 ggaaaagaag gggctggact cccgcaccgt gggtgagggc gagacagtca ccgcccaggt
     7141 gcccgcggcc ggctccgtca tccccggtga gagcaccgtg atcctctatc tgggcggcgc
     7201 ggagccggag gagacgggca ccgtgcccga cgtcaccggg atgagcttcg cagcggcccg
     7261 ctctaagctg gagtccgccg ggttcttcat gcgggcagca ggagcggcca cctatctggg
     7321 ctcctccagc aaggctcagg gccagagcgt ggccggcgga gagactgcgg ccatcggcac
     7381 catcgtagac gtcacgttca gcacacctca ggtggaggac ggatacctct acgacggata
     7441 agaagacaca ggcggagaaa gacctgattt tttgcggcgg tctttcctta tttcgcacct
     7501 cttaaagaaa atgaaaccca cccttgaggg gtgacaccat gaagctgcga gatctgctcg
     7561 ccggcgtccc cctcacgggg ggcaacgccg acctgaatat ggaaataaat tccatatcgt
     7621 atgacagccg gaccctggag ccgggcgccc tgtttgtgac gctctcaggg tccaagacag
     7681 acggacaccg gtatgtggcc caggcgctgg aaaagggcgc cgccgccatc ctgtgccggg
     7741 agccgccccg gacagggagc tggctctcca ctccagaccc cagggccgcc ctggcccggg
     7801 tgtccgccgc ttggttcgga tacccggggg aggagatgac cctgatcggg gtcaccggca
     7861 ccaacgggaa gaccaccacc acctatctta tcaaggccat gctggaaggg gcgctgaaga
     7921 cccgtgtggg cctgattg
//
LOCUS       DBMDSJ010000025         7719 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2897_length_7719_cov_1.843660, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000025 DBMDSJ010000000
VERSION     DBMDSJ010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2897_length_7719_cov_1.843660"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(139..510)
                     /locus_tag="ACZPAQ_01255"
     CDS             complement(139..510)
                     /locus_tag="ACZPAQ_01255"
                     /inference="COORDINATES: protein motif:HMM:NF040311.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HIR0902632.1"
                     /translation="MKKLERNIPGVYDFDEVLDYLHRGIMDMRTNVVWSLIQMQDTVT
                     LAGQWDGRRDGVRCAFRVYEKYIYTHSRGYRLTLTMMLAGRDGRLFLSVVPGASHQYA
                     DIAGVDETMDWIRTLADQLTG"
     gene            complement(687..1046)
                     /locus_tag="ACZPAQ_01260"
     CDS             complement(687..1046)
                     /locus_tag="ACZPAQ_01260"
                     /inference="COORDINATES: protein motif:HMM:NF040311.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HIR0902633.1"
                     /translation="MAKFECVFPSGYSFNDILQYFHTHLTKNHGECTGSSDYSSERAR
                     VAVRVYERYSLMGDNYLSLTLTLVNDGGDTFASAISAAGRGGGLGISIHWGEDKFLEI
                     FSDGANMFQGMRVLEKG"
     gene            complement(1071..1679)
                     /gene="ruvA"
                     /locus_tag="ACZPAQ_01265"
     CDS             complement(1071..1679)
                     /gene="ruvA"
                     /locus_tag="ACZPAQ_01265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR0902634.1"
                     /translation="MFYYLSGTVTHIEPYLAVIDCGGVGYACRTTSYTLSALKKGDKG
                     KLFTHLNVREDAMELYGFATQEELNLFQQLISVSGVGPKAALSILSASTPANLALSII
                     TGDEKALTCAQGIGKKIAQRVILELKDKLAKGQTINGAGETYGGTGVTVIPENKLSEA
                     SAALAVLGYSQGEINLALKGIDLDALTLEEVIKQALKKMMKG"
     gene            complement(1754..2206)
                     /locus_tag="ACZPAQ_01270"
     CDS             complement(1754..2206)
                     /locus_tag="ACZPAQ_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902635.1"
                     /translation="MPVIVKQPAPDLPYSLHDAYILEVRLEGDTLRLVTQYGYVETTE
                     PFGQVQGDVEITGVDWDSSHAYVMEYRGVLCGNRGPFTGRKMAVEDFLKTYPQGTLDI
                     MDETYGYRQVKLDGFLNLTGRCLEFRLELYYSGEFRYLLKELAPGAAV"
     gene            complement(2267..2788)
                     /gene="ruvC"
                     /locus_tag="ACZPAQ_01275"
     CDS             complement(2267..2788)
                     /gene="ruvC"
                     /locus_tag="ACZPAQ_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR0902636.1"
                     /translation="MIVLGIDPGVATIGFGVVRAERQKNTLVQYGVITTPPGIPLSSR
                     LLQISDDMEELIRTFHPDEMAVEELFFTKNITTGIAVAHGRGVILLSAERLGVPVFEY
                     TPMQVKQAVAGYGGADKRQVMLMTQRLLNMKQVPRPDDAADALALAICHGRAATSLLN
                     TERVFDPMKYDTR"
     gene            3052..3555
                     /locus_tag="ACZPAQ_01280"
     CDS             3052..3555
                     /locus_tag="ACZPAQ_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0902637.1"
                     /translation="MAEQPLSPEEGFASILERYEKMVYGFALARTGSRADADDVFQEV
                     FLAYFQCGKHFQEEEHRRAWLLRTTLNFSRRVTSSPWRRRVVPLSERADAPVQFQTPE
                     QTGLWSALSSLPDGLRVPLYLFYFEELSTQEIARLLSLRPGAVRMRLSRGREHLRELL
                     KGDYFHE"
     gene            3548..4441
                     /locus_tag="ACZPAQ_01285"
     CDS             3548..4441
                     /locus_tag="ACZPAQ_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902638.1"
                     /translation="MNDPIFSQMKEQLIPSPAARAALEEGLSQPVRRPVHRHRCRCWV
                     LAACLLLAAALPLGHALLSPPLHSYVTVEEAAGQEQDASTQEAAPCPGLIISESGLGP
                     DTLPTEEGEQYDLSRLPASAQDGGYPAQQLPSAEGETSSDADTGEGALLLERLTREIQ
                     REYGNGTYPDWYGGGYLDSAGDVVVLLVQGCDTQALRSQVRDWAGGGVSFARARYSLA
                     SLTKLQEQICAQPSVKAVLSSCALDEENNRLVLSLTRADDEALSALAVLDPRDDAVYV
                     QAAAPASAESGTGTACPRASR"
     gene            complement(4819..6342)
                     /gene="abc-f"
                     /locus_tag="ACZPAQ_01290"
     CDS             complement(4819..6342)
                     /gene="abc-f"
                     /locus_tag="ACZPAQ_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIR0902639.1"
                     /translation="MSLIQIEHLYFTYDGGSEPVFEDLDLQLETDWKLGLVGRNGRGK
                     TTLLRLLEGGLEYRGQIRTGGLACRRFPCAVRETEVPVSQVLRDISPEAEEWQLVREL
                     SLLGLDEEVLERTFSTLSGGEQTRVLLSALFLDEGSWPMLDEPTDHLDEEGRELLAQY
                     LSHSRRGFLLVSHDRAFLDRCVDHVLALNRTGPELVKGNFSVWYQEKQDRDRREQAQN
                     QQLKGEIRRLEQAARRTSAWSDQVEKTKYASKNSGLRPDRGYVGHKSAKMMKRAKSLE
                     SRQESAIQEKAALLHDVERAEPLKLAPRAFHSARLLELDRVSIDYGDGPVCGEVSFSL
                     SQGERLCLEGRNGSGKTSLLRLILGEEVPHTGTVRRASGLEISYVSQKVDHLQGALRD
                     FPAEEGIDATQFMTILRKLDFPRAAFEGEMGAYSAGQKKKVLLAASLCRSAHLYVWDE
                     PLNFVDLFSRIQMEELLLEYRPTLLFVEHDEAFRERVATRRVSLSERGLEKTDKDMK"
     gene            complement(6667..7548)
                     /gene="xerD"
                     /locus_tag="ACZPAQ_01295"
     CDS             complement(6667..7548)
                     /gene="xerD"
                     /locus_tag="ACZPAQ_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR0902640.1"
                     /translation="MSNLLEDYESYLTTEKKASANTVSSYLRDVHQFAQEMEERDVPL
                     DEVLPRDVEDYIRGLTRRGKSAATVTRSVASIKSFYNCLLSRGLVESNPARNVTAAKV
                     ERKLPQILTSREVELFLEQPDCSDLKGYRDRAMLELLYATGIRVSELIELDVDDLNLY
                     AGVLHCASKGKERVIPLYPAAIRALEEYLRNVRPQLINEPDETALFVNMSGDRMSRQG
                     FWKLIKYYQEKAGIQKDITPHTLRHSFAAHLLENGADLRSIQEMMGHADISSTQIYSR
                     MLNHKLKDVYQKAHPRA"
BASE COUNT         1382 a         2600 c         2248 g         1489 t
ORIGIN      
        1 acactccctc ccccgtcggt aacagactgg ctccccgcca ggatcccgtc ccggaaataa
       61 cggaagacct cgtccacgcc ccagcgggcg gaaaactcac gctccagcgt tgccatcgtg
      121 tcccctcctt cggcggactc agccggtgag ctggtccgcc agtgtcctga tccagtccat
      181 cgtctcatcc actccggcta tatcagcata ctggtgggac gcgcctggga caacggacaa
      241 aaacagcctg ccgtcccggc ccgccagcat catggtcagg gtaaggcggt aacctcttga
      301 atgagtataa atatatttct catacacccg gaaggcgcag cggacgccat cccgcctgcc
      361 gtcccactgg cccgccaggg tcaccgtatc ctgcatttgg atcaggctcc acaccacatt
      421 ggtccgcatg tccatgatcc cccggtgcag atagtccagc acctcatcaa agtcgtagac
      481 tcccggtatg ttccgctcca gcttcttcat cgtcccctcc tgttccggcc gcccacgggg
      541 cgggcctcac cgcagctcat gccactgcgg tctccagcgc ggcagaggag ccgccgtcca
      601 tcactgcccg gtcgatcagg gtcaaaacct gtctaaaatc gccgcggaag tgtttttcat
      661 atttggccgc cgcgtcccgg cacccttcag cctttctcca ggaccctcat cccctggaac
      721 atgtttgcgc cgtcggaaaa aatctccaaa aatttgtcct caccccagtg aatggagatc
      781 cccagaccgc ccccacgccc ggcagcggag atagcggatg cgaaggtatc cccgccgtca
      841 ttgaccaggg tgagcgtcag gcttagatag ttgtccccca tcagactgta tcgctcatac
      901 acccggacgg caacccgggc acgttcagag gaatagtcgc tgctccccgt gcattccccg
      961 tggtttttgg tgagatgggt atggaaatac tgcaggatat cgttaaagga atagccgctt
     1021 ggaaacacac attcaaactt ggccatttcc ctgcacctcc agttggaact ttaccccttc
     1081 atcatcttct tcagcgcctg cttaatgacc tcttccaggg taagggcgtc caagtcgatc
     1141 cccttgagcg ccaggttgat ctccccctgg gagtaaccca gcaccgccaa ggcggcggag
     1201 gcctcagaca gcttgttctc cgggatgacg gtgaccccgg tgcctccata ggtctccccc
     1261 gccccgttga tggtctggcc cttggccagc ttgtctttca gctccaggat gacccgctgg
     1321 gcgatcttct tgccgatgcc ctgggcgcag gtgagggcct tctcatcccc ggtaatgata
     1381 gacagagcaa ggttggccgg ggtgctggcc gacaggatgg acagggcggc cttggggccc
     1441 acgccggaga cagaaatgag ctgctggaac aggttcagct cctcctgggt ggcgaagccg
     1501 tagagctcca tagcgtcctc ccgcacgttg agatgtgtaa agagttttcc cttatccccc
     1561 ttcttcaggg cggacagggt atagctggtg gtgcggcagg catagcccac tccgccgcag
     1621 tcgatgacag ccagataggg ctcgatatgg gtcacggttc cactgagata ataaaacata
     1681 agataggacc tcacattaga tcagatagga gttatgagat atggatgcgg tatccagctc
     1741 cgccggacac agttcagaca gccgctccag gcgccagctc cttgagcagg tatcggaatt
     1801 ctccgctata atagagctcc agccgaaact ccaggcagcg gccggtcagg ttgagaaagc
     1861 cgtccagctt cacctggcgg tacccgtagg tctcgtccat aatgtccagc gtcccctgag
     1921 ggtatgtctt caaaaaatcc tccaccgcca ttttccggcc ggtaaagggc cctcggtttc
     1981 cgcagaggac gccgcggtac tccattacat aggcgtggga gctgtcccag tctaccccag
     2041 tgatctccac atccccctgc acctggccga agggctctgt tgtctccaca tagccgtact
     2101 gggtgaccag ccgcagggta tccccctcca gccgcacctc cagaatatag gcgtcgtgga
     2161 gactgtaggg caggtctggg gcgggctgct ttacgatgac aggcatgggt cccatccttt
     2221 ccgggagtca agtccccctg gtcctatctc ctgatccgct gttctttcat cgtgtgtcgt
     2281 atttcatggg gtcaaagacc cgttcggtgt tcaggaggct ggtggcggca cggccgtggc
     2341 agatggccag ggccagggca tcggcggcgt cgtcgggccg gggaacctgc ttcatgttca
     2401 gcaggcgctg ggtcatcagc atgacctgcc gcttgtccgc cccgccgtag cccgccacag
     2461 cctgcttcac ctgcatgggg gtgtactcaa agacggggac ccccagccgc tccgccgaga
     2521 ggaggatgac cccccggccg tgggccaccg cgatgccggt ggtgatgttt ttggtgaaga
     2581 acagctcctc caccgccatc tcgtccgggt ggaaggtgcg aatgagctct tccatatcgt
     2641 cagagatctg gagcaggcgg ctggacaggg ggatgcccgg cggggtggtg atgacgccgt
     2701 attggaccag cgtgtttttc tgacgctccg cacgcaccac cccgaatccg atggtggcca
     2761 cgcccgggtc gatgcctaaa acgatcatgg gcccctcccg cttccataca taccaagtaa
     2821 gtgtatcatg tttttctcca aatggaaaga ctgttttcac tttccctctc tctttcgata
     2881 ccgcggcaca ctgggcggct gtcgggaggc atgggaagag gcactccctt gctttcccag
     2941 cgggtgtatg gtacaacgga aaataggatg ggggttctgg agaaaaaagt ctgcttccac
     3001 cgtgacattt ccccctgcaa aatggagtat tatgcgaagg gaggtcgtga tatggcagag
     3061 caaccgctga gcccggagga gggattcgcc tccatcctgg agcggtacga aaaaatggtg
     3121 tacggcttcg cactggctcg gaccggcagc cgggcggatg cggacgacgt gtttcaggag
     3181 gtatttctgg cctatttcca gtgcggaaaa catttccagg aggaggagca ccgcagggcc
     3241 tggctcctgc gcaccaccct caatttcagc cgccgggtga cctcctcccc ctggcggagg
     3301 cgtgtggtcc ccctctcgga gcgtgcggat gcgccggtgc agttccagac cccggaacag
     3361 accgggctct ggtccgccct ctcctctctg ccagacgggc tgagggtccc cctctacctg
     3421 ttctactttg aagaactgtc cacccaggag atcgcgcggc tcctctccct ccgcccaggc
     3481 gcggtgcgga tgcgcttgtc ccgggggcgg gagcacctgc gggagctgct gaaaggagac
     3541 tattttcatg aatgacccca tcttttctca gatgaaggaa cagctcatcc ccagcccggc
     3601 ggcccgggcc gcgctggagg aggggctctc ccagcctgtc cgtcgccccg tccaccgcca
     3661 ccggtgccgg tgctgggtgc tggccgcctg cctccttctg gcggccgccc tgccgctggg
     3721 acacgccctc ctctctccgc ccctccacag ctatgtgacg gtggaggaag cggcggggca
     3781 ggaacaggac gccagtaccc aggaggcagc cccctgcccc ggcctcatca tcagcgagag
     3841 cggacttgga ccggacaccc tccccacaga ggagggggag cagtacgacc tcagccggct
     3901 tcccgcctcc gcccaggacg gcggctaccc tgcccagcag ctgcccagtg cggagggaga
     3961 gacctcttcc gatgccgaca caggagaggg cgccctgctc ctggagcgcc tgactcggga
     4021 gatccaacgg gaatacggca acggcaccta cccggactgg tacggcggcg gctatctgga
     4081 cagcgcggga gatgtggtgg tcctgctggt ccagggctgc gacacacagg ccctgcgcag
     4141 tcaggtccgg gactgggccg ggggcggggt gtcctttgcc agggcccgct actccctcgc
     4201 ctccctcacc aagctccagg agcagatctg tgcccagccc tcagtgaagg cggtgctgtc
     4261 ctcctgcgcc ctggacgagg agaacaaccg tctggtcctg tccctcacca gggccgacga
     4321 tgaggccctg tctgcactgg ccgtgctgga ccccagggac gacgccgtct atgtccaggc
     4381 ggccgctcca gcctctgcgg agtcaggcac cggcaccgcc tgcccccggg cctcccgcta
     4441 actgcgcttt ggaccgccca ctttcccaca tcgtcagggc tccccggcac tgccggggag
     4501 cccctctgtt atgcagatat cggccaccgg gcccagccgg atcggcggaa gaattcctcc
     4561 agctggcctc tgtcgatggt cacgacaacg ctggagggag atccgatggt gatggtggaa
     4621 cttgatcgac ctccctatgc gcagcagccc catgaggacc agcaggatcc caaccaggat
     4681 ggcggctgtg gacatcagca tctgaggcaa cagtctttcc acactcattc ctgccttttg
     4741 ccctgactcc cccctctccc gtggctgcca agtcttctcc tgcctctata cgtgcagggg
     4801 atggaaaaac tgcaaatctt atttcatatc tttgtctgtt ttttccagcc cgcgctcaga
     4861 cagggacacc cgccgggtgg ccacccgctc ccggaacgcc tcgtcgtgct ccacgaacag
     4921 cagggtgggc cggtactcca gcagcagctc ctccatctgg atacgggaga acaggtccac
     4981 aaaattcagc ggctcatccc agacatacag gtgggcgctg cggcacaggc tggccgccag
     5041 caggaccttc ttcttctggc ccgcgctgta cgcacccatt tctccctcaa aggccgccct
     5101 ggggaagtcc agtttgcgca ggatggtcat aaactgggtg gcgtctatcc cctcctccgc
     5161 cgggaagtcc cgaagggctc cctgcagatg gtccaccttt tgggagacat aggagatctc
     5221 caggccagag gcccgccgca cggtccccgt gtggggcact tcctccccca ggatcagccg
     5281 gagcaggctg gtcttgccgc tcccattcct cccctccagg cacagccgct ccccctggct
     5341 cagggagaag ctgacctcgc cgcacacagg cccgtccccg tagtcgatgg agacgcggtc
     5401 cagctccagc aggcgggcgc tgtggaacgc ccggggggcc agcttcaggg gctcggcccg
     5461 ctccacgtcg tggagcagag ccgccttctc ctggatggca gactcctgcc ggctctccag
     5521 gctcttggcc ctcttcatca tcttggccga cttgtggccc acatagcccc ggtcggggcg
     5581 cagtccggaa tttttactgg cgtatttggt cttttccacc tggtcggacc aggcagaggt
     5641 ccggcgggcc gcctgctcca gtcggcggat ctcccccttc agctgctggt tctgggcctg
     5701 ctcccgccgg tcccggtcct gcttctcctg gtaccacacg gagaaatttc ccttcaccag
     5761 ctccggcccc gtccggttca gggccagcac atggtccacg caccggtcca gaaacgcccg
     5821 gtcgtgggag acaagcagga agccccggcg gctgtggctg agatattggg caagcaactc
     5881 ccgcccctcc tcgtccaggt ggtcggtggg ctcgtccagc atgggccagc tcccctcgtc
     5941 cagaaacagg gcggacagga gcacccgggt ctgctcccct ccgctcaggg tggagaaggt
     6001 gcgctccagc acctcctcat ccagccccag cagggacagc tcccggacca gctgccactc
     6061 ctctgcctcc ggtgagatat cccgcagcac ctgggacacc gggacctccg tctcccggac
     6121 ggcgcagggg aaccggcggc aggccaggcc ccccgtgcgg atctgccccc ggtactccag
     6181 cccgccctcc agaaggcgca gcagggtggt cttccccctg ccgtttcggc ccaccagccc
     6241 cagcttccag tctgtttcca gctgtaggtc caggtcctca aagaccggct cgctgccgcc
     6301 gtcataggta aaatacaaat gttcgatttg gatcaaagac actgttctgc ctccctttcc
     6361 gccccgccgc cggcggggca tatgaaaaaa gccgcaagaa ggcgcacctt ctcacggctt
     6421 tggcagacga tccacagccg cccccctggg gcagctccat acgaacagcc ggagcgggaa
     6481 gacaacaccc tcttgctcag gcatgacaga accggggctt ttcaggcccc tctctcttcc
     6541 atcatgcggt tcagcaagcg gcattgcgca ttttcccgcc tccatttctt ctagattccc
     6601 atgcagtgta gcacagcgga gccgccctgt caagcaaaaa ccaggacgaa ctgcatccgg
     6661 cctggctcat gctctggggt gggccttctg atagacgtcc ttcagcttgt ggttcagcat
     6721 ccgggagtag atctgagtgg aggagatgtc cgcgtgcccc atcatctcct ggatggagcg
     6781 caggtccgcc ccgttctcca gcaggtgggc ggcgaaggag tggcgcaggg tgtggggcgt
     6841 gatgtccttc tggatgccgg ccttctcctg gtagtatttg atcagcttcc agaagccctg
     6901 gcggctcatc cggtccccgc tcatgttgac gaacagcgcc gtctcgtccg gctcgttgat
     6961 gagctgcggg cggacgttgc gcagatactc ctccagcgcc cggatggcgg cggggtaaag
     7021 ggggatgacc cgctccttcc ccttgctggc acagtgaagc accccggcgt agaggttcag
     7081 gtcgtccacg tccagctcga tcagctccga cacccggata cccgtggcgt agagcagctc
     7141 cagcatggcc cggtcccggt accccttcag gtcactgcag tctggctgct ccagaaagag
     7201 ctccacctcc cggctggtga ggatctgggg cagcttgcgc tccaccttcg ccgcagtcac
     7261 gtttcgggct gggttgctct ccaccagtcc ccttgacagc aggcagttgt agaaggactt
     7321 gatggatgcc acggaccggg tcaccgtggc agcggatttg ccccgccggg tcaggccccg
     7381 gatatagtcc tccacgtccc ggggcagcac ctcgtcgagc gggacgtccc gttcctccat
     7441 ctcctgggca aactgatgca cgtcccgcag ataggagctc accgtgttgg ccgaggcctt
     7501 cttttcagtt gtcagatagc tctcatagtc ctccaacaga ttcgacacac acaaaactcc
     7561 ctgattccag attgcgggcc ctcccgcaag tcacagcaca aagcgggccg aagcccccac
     7621 cagcaccgga acgatgctca gctccaagat cacacacagc atgactgccc cgccgcacag
     7681 cgcgcagcgg accccgtagg cggtgtcata gctgagggg
//
LOCUS       DBMDSJ010000026         7704 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2905_length_7704_cov_1.618046, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000026 DBMDSJ010000000
VERSION     DBMDSJ010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_7704_cov_1.618046"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="radA"
                     /locus_tag="ACZPAQ_01300"
     CDS             complement(<1..1001)
                     /gene="radA"
                     /locus_tag="ACZPAQ_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR0902641.1"
                     /translation="MKAKTVFYCTECGNETPKWQGRCPACGAWNTVVERPAEQIPRKG
                     GPARGTSGIGVPLSRPKSMEEVEVTDELRFRTGMGELDRVLGGGAVRGSLVLVGGAPG
                     IGKSTLMLQICDNLCRFAKVLYVSGEESERQIKLRAQRLNVAGEELYLLSETNLEHVL
                     DAVHSLQPDVLIVDSIQTLYHGDISSGPGSISQVKECTMSLMQLAKGEGITVFVIGHV
                     NKEGSIAGPKVLEHMVDCVLYFEGERQMAYRILRAAKNRFGATNEIGVFEMADAGLTE
                     VPNPSEALLAGRPTDAPGTCVTCVMEGVRPVLAEVQALVVPSSLGNPRRVSNGVDYNR
                     "
     gene            complement(1011..1733)
                     /locus_tag="ACZPAQ_01305"
     CDS             complement(1011..1733)
                     /locus_tag="ACZPAQ_01305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR0902642.1"
                     /translation="MKHVISVLCVLAVLLCMAPFVSPNGEGEAVSAAARTETGPFIVD
                     AGHGGEDGGAVSADGTQESGINLAIALRLEDILAFCGAAPVMLRREDISLHDPEAGSL
                     KEKKSSDLRNRTRAVQDLSGATLISVHQNSYPESRYDGAQVFYAPTEGSQALAEYTQE
                     ILRQTLDPDNGRQAKQIPDTVYLMNHIDNRAILVECGFLSNPQEAARLRDPVYQTKIA
                     AALASACLTFQGERITAHTTEI"
     gene            complement(1928..3406)
                     /gene="spoIVA"
                     /locus_tag="ACZPAQ_01310"
     CDS             complement(1928..3406)
                     /gene="spoIVA"
                     /locus_tag="ACZPAQ_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR0902643.1"
                     /translation="MEDRKIYEDIALRTEGDIYIGVVGPVRTGKSTFIKRFMETLVIP
                     NIENVYRRERARDELPQSGSGRTIMTAEPKFVPEEAVAVTMEGGAAFQVRLIDCVGYM
                     VEGAVGHLEGETQRMVTTPWFDHEIPMSEAAEIGTRKVIAEHSTIGIVITTDGTITEI
                     PREDYLEAEDRVITELKELGKPFLVLLNSAYPNSERAQAIQADISQRYGVACRALNCL
                     ELSEGDINTVLKDVLYEFPVKELDLFLPPWVDALAQDHPIKSALYTAIREGASDLYRI
                     RDVEQTVRSIKECEEVSDARVTSIDLGTGLAAAVLDLPRALFYHTLSQQSGFQIQDDG
                     DLVSLLTQLAGVKAAYDKVADALKEVEETGYGIVVPSIDSLVLEEPEIVRQGGRYGVR
                     LKASAPSIHMIRADIETEVAPIVGTEKQSEEMVHYLLQEFEGDTSKIWQSNIFGKSFY
                     ELVGEDLNTKVKRMPEDAREKLRETLQRIINEGSGGLICIIL"
     gene            complement(3565..5085)
                     /locus_tag="ACZPAQ_01315"
     CDS             complement(3565..5085)
                     /locus_tag="ACZPAQ_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR0902644.1"
                     /translation="MELELKRDAIHCFETVADVTICQEETQEAIVPDACPDILRIVEV
                     CGQACLTGKQAREGMATVSGMVRASVLYLPETGGCLRHMELSVPFTCQVEAPGLTSQG
                     TILADPRLRWAEARVLNPRKILLRVDLAVEIRACQPCERTICQGVEAAEEHGIRQLQE
                     EQTTDQIVAVQEKPFTFTDGVDLGLSEGGELLSLRGEPSCEESRLIGSKLIFKGSVEL
                     HALLRDADGTLRTARESMPFSQIIEVPGAGEDAECEVRVVLTDLNAPAIGTTVGSAEV
                     TLELLAQAVVRSQRPVTMLQDIYSTGWMLEVQTEEHTMRQVVDQSIRPQSVREILETT
                     TLVRNAIDGWLSLGEIRQSREGDQMILSAEVLITALYLDDGEELQSIQRTVQTDCRLD
                     CPVQAVCQCWCTAPGELAVVPAAGGLEVRFTLEFHCLCLAERRQAAISSARLGEERAK
                     PDGTQPSVVLRMAAPGERLWDIAKAYGTTTEEITQANELEEGCLPTGRMLLIPRVR"
     gene            complement(5396..6274)
                     /gene="rsgA"
                     /locus_tag="ACZPAQ_01320"
     CDS             complement(5396..6274)
                     /gene="rsgA"
                     /locus_tag="ACZPAQ_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR0902645.1"
                     /translation="MAEGMILKALSGFYYVDDGTQLFACRGRGKFRHEKVTPLVGDRV
                     LFTPLEHGSGSLDGILPRKNEFQRPAVANVDQLVIIVSQAVPATDPFLIDRVISIAER
                     RGCDSVICVNKWDLVPGEDLYQIYTSAGFTTLKVSAETGEGIEELRQVISGKVSAFTG
                     NSGVGKSSILNALEPGIALQTGEISEKLGRGRHTTRHVELYRLSGGALVADTPGFSSF
                     DTDRMELCRKEELEGCFREFAPYLGKCQFTGCSHVKEKGCAVLKAVEAGAIQPSRHAS
                     YIRLYDQAKQIPDWAR"
     gene            complement(6276..>7704)
                     /locus_tag="ACZPAQ_01325"
     CDS             complement(6276..>7704)
                     /locus_tag="ACZPAQ_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR0902646.1"
                     /translation="YSAGVVLYEMITGRLPFEGDTPVSVAIQHINSIPLSPRELDPTI
                     PEALEAITMKAMAPNPDNRYPSADAMLADLEEFRKNPSINFDYDVSEFVPEEPEEGAM
                     TQIRTPHTPVRSRVPEPEPRRRRYEEPEEEEEELENRPRRRDRRRGGTNWPIIAAIVV
                     ILAFVAGLFYFMYQFFISSILMPSPDYTIPKLTGYTYEEVLQDPELRQEFNIVVGQTV
                     ESEEAAGTILRQDPAAGSTVKSNNAEITVTVSGGPEVIQMIDVTNQEYLKALATLRDM
                     GLSVEAPEYQFDDEVPANYIISYTPAEGTELKAGDSVHLVVSKGPEASKVTLISFADM
                     AQSDAESRIRLMNLEVGEIEHVSSETVPEGKVVEQWPEAGTEVEEGSKVNLTVSTGPE
                     NPDIPQTSTKTLTINLPVVDETDPVQSINVMVTSDDGKEVYNRDVNPLMEVAVSVPVS
                     GTGKVTYNVYFNGRLDHGVTVNFDE"
BASE COUNT         1371 a         2522 c         2233 g         1578 t
ORIGIN      
        1 cggttgtagt ccaccccgtt gctcacccgg cggggattgc ccaggctgga gggcaccacc
       61 agcgcctgta cctccgccag cactggccgc accccctcca tcacgcaggt gacgcaggtc
      121 ccgggggcgt cggtgggccg cccggccagc agggcctccg acgggttggg cacttccgtc
      181 agtcccgcgt ctgccatctc aaagacgcca atctcattgg tggcgccaaa gcgatttttc
      241 gccgcccgga ggatgcggta ggccatctgc cgctctccct caaaatagag aacgcagtcc
      301 accatatgct ccagcacctt agggccggcg atagatccct ccttgttcac gtggccgatg
      361 acaaagacgg tgatcccctc ccctttcgcc agctgcatca gggacatggt acactccttc
      421 acctgggaga tgcttcccgg ccccgagctg atatccccgt gatagagggt ctgaatggag
      481 tccacgatga gcacatcggg ctggaggctg tggactgcgt ccagtacgtg ttccaggttt
      541 gtttccgaca ggagatagag ctcctcgccg gccacgttca gccgctgggc ccgcagcttg
      601 atctgccgct ctgactcctc accggataca tacaggacct tggcgaaccg gcacaggttg
      661 tcgcagatct ggagcatcag ggtggatttt ccaatgcctg gtgcgccgcc caccagcacc
      721 aatgaaccgc gcaccgctcc gccgcccagc acccggtcca gctctcccat cccggtgcgg
      781 aagcgcagct catcggtgac ctccacctct tccatggact taggacggct gagagggacg
      841 ccgataccgg atgtcccccg ggcgggccct cccttcctgg ggatctgttc tgcgggccgc
      901 tccaccaccg tattccaggc cccgcaggcc ggacatctcc cctgccactt gggcgtctca
      961 tttccacatt ccgtacaata aaataccgtc ttggccttca tatctcatcc tcaaatctct
     1021 gtggtatggg ccgtgatccg ctccccctga aaggtcaggc aggcgctggc cagggctgcg
     1081 gcgatcttgg tctgatagac cgggtctcgg agcctggcgg cctcctgtgg attgctgagg
     1141 aagccgcact ccaccaaaat cgcccggttg tcaatgtgat tcatcagata tactgtgtct
     1201 gggatctgct tggcctgcct gccgttgtcc gggtccagcg tttggcgcag gatctcctgg
     1261 gtgtattcgg ccagtgcctg ggagccctct gtgggtgcgt agaacacctg tgcaccgtcg
     1321 tagcggctct ccgggtagct gttttggtgg acactgatca gggtggcgcc ggacagatcc
     1381 tggacggccc gggtccggtt gcgcaggtcc gaggacttct tctccttcag ggaaccggcc
     1441 tccgggtcgt gcagggagat atcctcccgg cgcagcatga cgggggcggc accgcagaaa
     1501 gccaggatgt cctccagccg aagggctatg gccaggttga tgccgctctc ctgggtgccg
     1561 tctgccgaga ccgcaccgcc gtcctctcct ccgtggccgg catccacgat gaaggggcct
     1621 gtctccgtgc gggcagcggc ggagacagcc tctccctctc cgttcggaga cacgaagggg
     1681 gccatacaca gcagtactgc caggacgcac aggaccgaaa tgacatgttt catattggca
     1741 tacacctccc gtccatccta tggccgggag gccgctttca tgttattata ccgcagatag
     1801 gtccaaaagg caaaggctca tgcccacatt agctgcggta ttttggaaag gactataaag
     1861 cagttctctc ccagttgaca taatataaga gggcggggaa cttagatgcg ccctgccctc
     1921 ttatttgtta caggatgatg cagatcagtc cgccggagcc ctcgttgatg atgcgctgga
     1981 gggtctctcg gagcttttcc cgggcgtcct ccggcatccg cttgaccttg gtgttcaggt
     2041 cctcccccac cagctcgtag aaggatttcc cgaaaatgtt ggactgccag atcttggagg
     2101 tgtccccctc aaattcctgg agcagatagt ggaccatctc ctcgctctgc ttctccgtac
     2161 ccacgatagg ggcaacttcg gtctcaatat ccgcacggat catatggatg gagggggcgc
     2221 tggccttgag gcggacgccg tagcgtcccc cctgccgaac gatctccggc tcctccagca
     2281 ccagggaatc gatggagggc accacgatgc cgtagccggt ctcctccacc tccttcagag
     2341 catctgccac cttgtcatat gctgccttga ctccagccag ctgggtcagc agagagacca
     2401 gatctccgtc gtcctggatc tggaagccgg actgctggga cagagtgtgg tagaacaggg
     2461 ccctgggcag gtccagtacc gcagcggcca gcccggtgcc taggtcgatg gaggtcaccc
     2521 gggcgtcgct cacctcctcg cactccttga tggagcggac cgtctgctcc acgtcccgga
     2581 tgcggtacag gtcggaggcc ccctcccgga tggcggtgta cagggcggac ttgatggggt
     2641 ggtcctgagc cagggcgtcc acccaggggg gcaggaacag gtccagctcc ttcaccggga
     2701 actcgtagag cacatccttc aggacggtgt tgatgtcccc ctcgctcagc tccaggcagt
     2761 tgagtgcccg gcaggccacc ccgtaccgct ggctgatgtc cgcttggatg gcctgggccc
     2821 gctccgagtt gggataggcg gagttgagga ggaccagaaa gggcttcccc agctccttca
     2881 gttccgtgat gacccggtcc tccgcctcca gatagtcctc ccggggaatc tcggtgatgg
     2941 tcccgtccgt ggtgatgacg atgccgatgg tggaatgttc cgcaatgacc ttccgggtgc
     3001 cgatctccgc cgcctccgac atggggatct cgtgatcgaa ccacggggtg gtcaccatcc
     3061 gctgggtctc tccttccaga tgtcccaccg ctccctccac catgtagccc acgcagtcga
     3121 tgagccggac ctggaaggcg gcgcccccct ccattgtgac agctaccgct tcctccggga
     3181 caaatttggg ctccgccgtc atgatggtac gcccggagcc gctctggggc agttcatccc
     3241 tggcccgctc ccggcggtac acattttcaa tgttggggat gaccagcgtc tccatgaacc
     3301 gcttgataaa tgtggatttt ccggtccgga ccggccccac gacgccgata tagatatcgc
     3361 cctcggtccg aagcgcgata tcctcgtaga ttttgcgatc ttccactgaa attcctcctt
     3421 cggccgagca agccggtgat ttccttacag tgtatgggga taagctgtgg aatatgacct
     3481 tgccgcatag cttttccagc ctttgtttga aaaaggagcc ggaccgcagg ccgacaggcc
     3541 cggtccggct ccttgccgat gagatcagcg tacccggggg atcaggagca tcctgcccgt
     3601 gggcaggcag ccctcctcca gctcattggc ttgggtgatc tcctctgtgg tagtaccgta
     3661 cgctttcgcg atgtcccaca gccgctctcc cggagcggcc atacgcaaca ccacagaggg
     3721 ctgtgtcccg tccggcttgg cccgttcctc ccccagacgc gcggaggaga tagcggcctg
     3781 cctgcgctcc gccagacaga ggcagtggaa ttccagagtg aagcgcacct ccagtccgcc
     3841 ggccgccggc accacggcca gttcgcctgg ggcggtacac cagcactgac acaccgcctg
     3901 gaccgggcag tccagcctgc agtccgtctg gacggtgcgc tggatagact gaagctcctc
     3961 cccgtcatcc agatacaggg ccgtgatgag tacctctgcc gaaaggatca tctggtcgcc
     4021 ctcccggctc tgccggatct cccccaggga cagccacccg tctatggcgt ttcgtaccag
     4081 ggtggtggtc tccaggatct cacggacgct ctggggccga atggactgat ccaccacctg
     4141 gcgcatggtg tgttcctcgg tctggacctc cagcatccat ccggtgctgt aaatatcctg
     4201 gagcatggtg accggccgct ggctcctcac caccgcctgg gccaggagct ccagcgtcac
     4261 ctcggcggag cccaccgttg tcccaatggc gggggcattc agatcggtaa ggacgacccg
     4321 cacctcacat tccgcgtcct ctccggcacc aggaacttcg atgatctgag aaaagggcat
     4381 ggactccctg gctgtccgaa gggtcccgtc cgcgtcccgc aggagggcgt ggagctccac
     4441 actgcccttg aagatcagct tgctgccaat gagacggctc tcctcacagg atggctctcc
     4501 ccggaggctg agcagctcac caccctctga tagccccaag tccacaccat ctgtaaatgt
     4561 aaagggtttt tcctgcacgg ctacgatctg atcggtggtc tgctcctcct ggagctggcg
     4621 gataccatgt tcctccgccg cctctacccc ttggcatatg gtgcgctcac agggctggca
     4681 ggccctgatc tccacagcca ggtccacccg gagcaggatc tttctgggat tcagcacccg
     4741 ggcctccgcc caccgcaggc ggggatcggc caaaatggtc ccctgggagg tcaggccggg
     4801 ggcctcgacc tggcaggtga aggggacgct gagctccata tggcgcagac atccccccgt
     4861 ctccggcaga tacagcaccg aggcgcgcac catgccggag acggtggcca ttccctcccg
     4921 ggcctgtttt cctgtgaggc acgcctgacc gcatacctca acgatgcgca gaatgtccgg
     4981 acaggcatcc ggaacgatag cctcctgggt ctcctcctga cagatggtga catctgctac
     5041 ggtctcaaag cagtggatcg catctcgttt gagctcaagc tccataatcg tcctctccct
     5101 tacaaatcga gtcggcgcgg cctgggccgc cgcgccgggt cagccattcc gctttgggac
     5161 cactatatgt acaggcaggg acaaatatgc caccctcaga aaaggactca gtgccccctg
     5221 ggccccagga tcacttcagg ccaaacagct tccgcagcag accccgcaga catctgggag
     5281 aacgtaccac gacgatgccc ataagaaagc ctcccctccg catagactgg ccgttttccg
     5341 gctaatccag tctatgcacg gggaggcgat gtgtcactgt tccactgata aaattttatc
     5401 gcgcccagtc cgggatctgc ttggcctggt cgtacaggcg tatatagctg gcgtggcggc
     5461 tgggctggat cgctccggcc tccaccgcct tcagcaccgc gcagcccttc tccttcacat
     5521 gggagcaacc ggtaaactgg cattttccca ggtaaggggc aaactcccgg aagcagccct
     5581 ccagctcctc cttgcggcaa agctccatgc ggtccgtatc aaaggaggag aaccccgggg
     5641 tgtcggccac cagagccccg ccgctcagcc ggtagagctc cacatggcgg gtggtgtgcc
     5701 gaccccgccc cagcttttcg ctgatctccc ctgtctgcag ggcgatccca ggctccagtg
     5761 catttaggat gctggactta ccgacgccgg aatttcctgt gaaggcagac acctttccag
     5821 aaatgacctg ccgcagctct tcgatcccct cgccggtctc cgcgctcacc ttcagggtgg
     5881 tgaagccggc ggaggtatag atctggtaca ggtcctcccc cggcaccaag tcccacttgt
     5941 tgacgcagat gacgctgtca catccccggc gctccgcgat ggagatcacg cggtcaatga
     6001 ggaagggatc ggtggcaggg accgcctggg acacgatgat gaccagttgg tccacgttgg
     6061 ccaccgccgg gcgctggaac tcattctttc tggggagaat cccgtccagc gagccgctcc
     6121 cgtgttccag cggggtaaac agcacccggt cacccaccag aggggtcacc ttctcatggc
     6181 ggaacttccc ccgcccccgg caggcgaaca gctgagtccc gtcgtccaca tagtagaagc
     6241 cgctgagggc ttttaaaatc attccttctg ccatatcact cgtcaaagtt gaccgtgacg
     6301 ccgtggtcca atctcccatt gaaatagacg ttgtatgtca ctttccctgt cccactgacc
     6361 ggtactgaca cagccacttc cataagaggg ttcacatccc ggttataaac ttcctttccg
     6421 tcgtcgctgg tgaccatcac attgatggac tggacagggt ccgtctcatc caccaccggc
     6481 aggttgatgg tgagtgtctt ggttgatgtc tgaggaatgt ccgggttctc aggccctgta
     6541 ctgacggtaa ggttgacctt gctcccctct tccacctctg taccagcctc cggccactgt
     6601 tccactacct ttccctccgg tactgtttca ctggagacat gctctatctc acccacctcc
     6661 aggttcatca gacggatccg gctctccgcg tcgctctggg ccatgtccgc gaaagagatc
     6721 agcgtgacct tggacgcctc aggcccctta ctgaccacca gatggacgct gtcccccgcc
     6781 ttgagttcgg tcccctccgc aggggtatag gagatgatat agttggcggg cacctcatca
     6841 tcgaactgat attcgggggc ctccacgctc agccccatgt cccgcagggt ggccagggcc
     6901 ttcagatatt cctggttggt gacatcgatc atctggatca cctcgggtcc accgctcacc
     6961 gtcaccgtga tctctgcgtt gttggacttc acggtgctcc cggcagcagg gtcctgcctg
     7021 aggatcgtcc ccgcagcctc ctcgctttcc accgtctggc caacgacgat gttgaactcc
     7081 tgtctcagct ccgggtcctg aaggacctcc tcataggtgt agcccgtcag cttggggatg
     7141 gtatagtccg gcgagggcat caggatggag gagatgaaga actgatacat gaagtagaac
     7201 agtcccgcca caaaggccag gatgacaacg atggccgcga tgatgggcca gtttgtccct
     7261 ccccgccggc ggtcccggcg cctgggacgg ttctccagct cctcctcttc ctcctccggc
     7321 tcctcgtagc ggcgcctgcg gggttcaggc tccggcacac ggctgcgcac cggcgtatgg
     7381 ggcgtgcgga tctgagtcat ggcgccctct tccggctcct cgggcacgaa ctcgctcacg
     7441 tcgtagtcaa agttgatgct ggggttctta cggaactctt ccaagtcggc cagcatcgcg
     7501 tctgcggagg gatatcggtt gtctgggttg ggagccatgg ccttcatggt gatggcctcc
     7561 agcgcctctg ggatggtagg gtccagctca cggggggaga gtgggatgga attgatgtgc
     7621 tggatggcca cggagaccgg agtgtccccc tcaaagggca ggcggccagt tatcatctca
     7681 tagagcacca cccccgcaga gtac
//
LOCUS       DBMDSJ010000027         7697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2910_length_7697_cov_1.790943, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000027 DBMDSJ010000000
VERSION     DBMDSJ010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_7697_cov_1.790943"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            16..1284
                     /locus_tag="ACZPAQ_01330"
     CDS             16..1284
                     /locus_tag="ACZPAQ_01330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024240.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0902647.1"
                     /translation="MQQETQFDLIVVGGGTAGAFSAIAAAREGLKVAVVERGTCLGGL
                     AASSGLTEMNAAGFQGAPLYRGIEREVFDRLIWGGHAAYHFAVPMSSNKEVKIDRLRY
                     DPERLKLLLEQLAVEAGITLLYETELTAAREGEEECQITARGPYQEIALSGKYLIDAT
                     GNASLVQALGGETEAADERERMIATLMFRISNVDLDQLDAFIHSPRLGETIRQGRESG
                     VLKGGILAFTPIPGTRDVSLNVTRAKFDHEDLADATRGMVELRSQIEPIFRFVQREVP
                     GLEGAYLSGIAPLAGVRDARRIRGAYRLTIEDLERMTCFEDRVACGCYPMDLHDPVTN
                     TVVWKMLPGVYHIPYRSLLPLGMKRTLAAGKCLSADPKAFGAVRVMPIMMNVGESAGY
                     AAALALRENKTLDQISSASLRSCLDQKYGV"
     gene            1309..2751
                     /locus_tag="ACZPAQ_01335"
     CDS             1309..2751
                     /locus_tag="ACZPAQ_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR0902648.1"
                     /translation="MKGKTSKLVMLLISFAFILLFQFIPAPAGLNQTSMQVIGIFIGI
                     MLMWNFIGTDWPSLLCMAILAIFQIMSPGDIFKSGFGNSTIAFLLVFFMLSHVLSQVG
                     LTRRLAIWFFTNKLAQRSPWMFVTMFLFGAMFMASFMSQTAALLVFLPIAEQVFKELK
                     YEKGDRFPQMLVLGLGIAVGIGSANTPLGHAIILIPIQLLESQTGLSVNIMAYSAFGI
                     VTGLLIFAALILVYRLLYRPDLNKLRHYDATRLRSDLQPMSVQEKISAVLFVVVIVIW
                     LLQGFLKDIAPTVGGYISALGNAVPVMVAIVILCLTKVDGKPIMDYKDAAARGVPWSA
                     LIFNAAVLVISGALTLEKVGISDFLISNVTPLVSGMNQTIFILVIATLCIIATNFSSN
                     TVCATVFYTISAPIALAMGNINMVALASVIGAAASYAFATPPSTMPMAVVAGTGWVDV
                     KDMFKYGGLMALLSIVILAFVGYPIAAAVL"
     gene            complement(2845..3786)
                     /locus_tag="ACZPAQ_01340"
     CDS             complement(2845..3786)
                     /locus_tag="ACZPAQ_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0902649.1"
                     /translation="MKRIILRGGALLELDQVRHVLAVVKYRTFLEASFQLNRSQSSLS
                     KSIRRLEEELGISIFERTTRRVELTPAGRDFVAYAEQMMVCYEGMLHAMEQHRSNGKR
                     SLRVGSIYFGLHNRLAPLVANFSKLHPSMEIEIRESTTTPLLQDLHKGELDVVFVSSM
                     YPEGAERANFSQYPEYRSHSCFRESYQIVVSRDHPFAGRSQLTYEDLADQPFIMTDRT
                     MDVYHRAVQKAFDAHRVPLHIAMYCTTVRSVLHMVSQNMGIAMLSPLVIEESDDLCII
                     PLKDALLRDTQMVILNQKEIPPHVRSFYHYVKTQNLA"
     gene            4025..5467
                     /locus_tag="ACZPAQ_01345"
     CDS             4025..5467
                     /locus_tag="ACZPAQ_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013132.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR0902650.1"
                     /translation="MSTKKTIATIIGVLLMFGCQFLPPVFGLTPLGMQVAGIFIGTIL
                     LWMFVSVTWPSILCIIALILSPLYTYSSGLSGSMGGWITSFVLFSSMVTYTLGQTGFL
                     KRCAVWFVTRPWAQKNPWLFLGLLFLAPLVIGSFMSPIPAFIVCLPIAEQIFKELKYE
                     KGDRFPQIVVLGILFFASLSTAATPIAHTVTTMALSLYENDAGVPVNFVSYTIFGVAA
                     CLVTFAASMVLTKFVCRPGTERLSSLDSSVLKSETTPMSRQEKYTLLVFVLVVAMWLL
                     PGIIAPVLPEVASAISSLGTPTPAMIGACLLCVLHVEGKPLMNFNEAVAKGVPWGAVF
                     MVAATSVLGSALTHEEAGITAVVSGALSPIIGSMSPIIFVLFIALATVMITNFASNTV
                     TVTLMYSISLPLVYGGAISGVDPAALTCVIGACACVACATPPSTAHAAIAAGSGWLNT
                     DTMLKYGLLWSVTAAVIFAVVGYPIAAAIM"
     gene            5490..6746
                     /locus_tag="ACZPAQ_01350"
     CDS             5490..6746
                     /locus_tag="ACZPAQ_01350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024240.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0902651.1"
                     /translation="MERQFDVIVVGGGTAGFAAAVTAARGGAKTLLIEEKAYLGGTAT
                     GAQVSMFMGFADGEPDRPQQGIIKDVMDGLAAAGGTSGIETIYLCGRRDLDIPVIPYE
                     SEILKDLIFDLVDQAGVELLLHTRVIGAQVEDGVITALTIHNEQGVQTVSGKVVIDAS
                     FHGSVAVSAGCRWEIGDEKGVLQPGTLMYKMAGVDIARYEQVSQPERERLAQKGIEEG
                     CLYVNNLLARPLPSGTIYSNMSRIRIDPLDAAQWSRAEMEARRQVRRISRFFIENVPG
                     FEKAYLISTGDFTGLRDSRRIQGKYVLTGEDVVEGKLFADPVVRSSYPVDIHDTDGVS
                     STIVKPKTGVFYIPYHCLVTNEISNLLLAGRCISTDYAAHACIRVMITCMRLGEAAGL
                     AAAESVHLGTAPNALDGRSISKQLGF"
     gene            6823..7596
                     /gene="aroD"
                     /locus_tag="ACZPAQ_01355"
     CDS             6823..7596
                     /gene="aroD"
                     /locus_tag="ACZPAQ_01355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR0902652.1"
                     /translation="MMKTVMLRNVEVGCGKPKVIVPIVAATAQGILEKAREFRAHALD
                     VVEWRVDFYEDVFQLPKVLSTLKGLREILGETPILFTFRTRKEGGEKEIDMEAYTALN
                     TAVAQSGDADAVDVEVFSGDEVVRRNIQAIHAAGKAVVGSNHDFGGTPDKADLLFRLR
                     KMQELGADIPKIAVMPRCPGDVIALLDATQEMSGKYADRPIITMSMGAGVVSRLCGEY
                     FGSAMTFGAVGQVSAPGQIPVEELNAAMDILHRALAPRS"
BASE COUNT         1437 a         2286 c         2431 g         1543 t
ORIGIN      
        1 gaaagaaggg agtacatgca gcaagagaca cagtttgatc tgatcgtggt gggaggcggc
       61 acagccgggg ccttcagcgc catcgccgcg gcccgggagg ggctgaaggt ggccgtggtg
      121 gagcggggga cctgcctggg cggcctggcc gccagctccg gtctgaccga gatgaacgcg
      181 gcgggctttc agggggcgcc cctctaccgg gggatcgagc gggaggtctt tgaccgcctg
      241 atctggggcg gacatgcggc ctaccacttc gcagtcccaa tgtcctccaa caaagaggtg
      301 aagatcgacc gcctccgcta tgacccggag cggctcaagc ttctgctgga gcagctggcc
      361 gtggaggcgg ggatcaccct gctctatgag acggagctga ccgcggccag agagggagag
      421 gaggagtgtc agatcacggc ccgggggccg tatcaggaga tcgccctctc cggaaaatac
      481 ctgatcgacg ccaccggaaa cgcctccctg gtccaggcgc tgggcgggga gacggaggcg
      541 gcggacgaga gggagcggat gatcgccacc ctgatgttcc gcatctccaa cgtggacctg
      601 gaccagctgg acgcctttat ccacagccca cgcctggggg agaccatccg gcagggccgg
      661 gagagcggtg tgctgaaggg aggcatcctg gcctttaccc ccatccccgg cacccgggac
      721 gtgagcctga atgtcacccg ggccaagttt gaccacgagg atctggccga cgccacccgg
      781 gggatggtgg agctgaggtc ccagatcgag cccatcttcc gcttcgtcca gcgggaggtg
      841 cccggcctgg agggggccta cctctccggc atcgcccctc tggccggtgt gcgggacgcc
      901 cggcgcatcc ggggggccta ccggctgacc atcgaggatc tggagcggat gacctgcttt
      961 gaggaccggg tggcctgcgg atgctatccc atggacctcc acgaccccgt gaccaacacc
     1021 gtggtgtgga agatgctccc cggggtctat catatcccct accgcagcct gctccccctg
     1081 gggatgaagc ggaccttggc ggccggaaag tgcctgtccg cggacccgaa ggcctttggg
     1141 gcggtccggg tcatgcccat catgatgaac gtgggagagt cggcgggcta tgcggcggca
     1201 ttggccctgc gggaaaacaa gactctggac cagatctcct ccgccagcct gcggagctgt
     1261 ctggatcaga aatatggtgt gtaagaaaga gaagggagag atccaaaagt gaaaggcaag
     1321 acctcaaaac tcgtgatgct gctgatctcc tttgcgttca tcctgctgtt ccagttcatc
     1381 cccgctcccg cgggcctgaa ccagaccagc atgcaggtca tcggtatctt catcggcatc
     1441 atgctcatgt ggaacttcat cggcaccgac tggcccagcc tgctgtgcat ggcgatcctg
     1501 gccatcttcc agatcatgtc gccgggggat atctttaaaa gcggctttgg caactccacc
     1561 atcgccttcc tgctggtgtt cttcatgctc agccacgtgc tgtcccaggt gggcctcacc
     1621 cgccggctgg ccatctggtt ttttaccaac aagctggccc agcgcagccc ctggatgttc
     1681 gtgaccatgt tcctcttcgg ggccatgttc atggcctcct tcatgtctca gaccgcggcc
     1741 ctgctggtct tcctgcccat tgcggagcag gtcttcaagg agctgaagta tgagaagggg
     1801 gaccgcttcc cccagatgct ggtgctgggc ctgggcatcg cggtggggat cggctccgcc
     1861 aacacccccc tgggccacgc catcatcctg atccccatcc agctgctgga gtcccagacc
     1921 ggcctgtccg tgaacatcat ggcatacagc gctttcggca ttgtcaccgg cctgctgatc
     1981 tttgcggccc tgatcctggt gtaccgcctg ctctaccgcc cggacctgaa caagctgcgc
     2041 cactatgatg ccaccaggct gcgctctgac ctccagccca tgagcgtgca ggagaagatc
     2101 tccgcggtcc tgttcgtggt ggtcatcgtg atctggctcc tccagggctt cctgaaggac
     2161 atcgccccca ccgtcggcgg gtatatctct gcgctgggca atgcggtgcc tgtgatggtg
     2221 gccatcgtga tcctctgcct gaccaaggtg gacggcaagc ccattatgga ttataaggac
     2281 gcggccgccc gcggcgtgcc ctggagcgcc ctgatcttca atgccgccgt cctggtcatc
     2341 agcggcgccc tgacgctgga gaaggtgggc atctccgact tccttatcag caacgtcacc
     2401 cccctggtct ccgggatgaa ccagaccatc ttcatcctgg tcatcgccac cctgtgcatc
     2461 atcgcaacca acttctcctc caacaccgtg tgcgccaccg tgttctatac catctccgcc
     2521 cccatcgccc tggctatggg caacatcaat atggtagccc tggcctccgt catcggagcc
     2581 gcggcctcct acgctttcgc cactcccccc agcaccatgc ccatggccgt ggtggccggc
     2641 accggctggg tggacgtgaa ggatatgttc aagtacggcg gcctgatggc cctgctgagc
     2701 atcgtgatcc tggcctttgt gggctacccc atcgccgcgg cggtgctgta agcgtccgcc
     2761 ccacggtgga tgccggaatg aaaagccccg tgaggtccgc tcaacggcag accccgcggg
     2821 gcctttcagg atatcgaaaa aaggctacgc cagattttga gtcttgacat aatgataaaa
     2881 ggatctcacg tggggcggga tctctttctg gttgaggatc accatctgag tgtcccgcag
     2941 cagggcatcc ttcaggggga tgatgcacag gtcgtccgac tcctcgatga ccaggggaga
     3001 gagcatggcg atccccatgt tctgggagac catatgcaga acagagcgga cagtggtgca
     3061 gtacatggcg atatgcaggg ggaccctgtg ggcgtcaaag gctttctgaa cggccctgtg
     3121 atagacgtcc atggtccggt ccgtcatgat aaagggctgg tcagccaggt cctcatatgt
     3181 gagctggctc cggcctgcaa aggggtggtc ccggctgacc acgatctgat aggactcccg
     3241 gaagcaggaa tgggagcggt attctggata ctgagagaaa ttggcccgct cagctccttc
     3301 cgggtacatg ctggagacaa agaccacatc cagctcccct ttatggagat cctgaaggag
     3361 cggcgtggtg gtggattccc ggatctcgat ctccatgctg gggtggagct tggaaaaatt
     3421 ggccaccaga ggggccaggc ggttgtgcag gccgaaatag atgctgccca cccggaggga
     3481 gcgcttgccg ttgctgcggt gctgttccat ggcgtggagc atcccttcat aacagaccat
     3541 catctgttcg gcgtaagcga cgaagtcacg gcctgccggg gtcagctcca cgcggcgggt
     3601 ggtgcgctca aagatggaga tccccagctc ctcctccagc cggcggatgc ttttggacag
     3661 ggaggactga gagcggttga gctgaaagga tgcctccaga aatgtgcggt attttaccac
     3721 cgccagcacg tggcggacct ggtccagctc caacagagca ccccctctca gaatgatcct
     3781 tttcattata gcatgacgcc tgccccgtta caatgagaat ggaccgaatg ttttgggggc
     3841 agagtggaaa ataccaggag aaagagaggg gaaaatgcac aaaaaaggaa taaaagattc
     3901 tatttattcc ttttgagaat aaatagagag ggaagaagaa ttgctcctga gatggtttgc
     3961 tctataattt gacacagaga acgagccggc ttgaaaatca gatcaggagg aatgtgagaa
     4021 gatcatgagt acgaaaaaaa cgatcgcgac tatcatcggc gtcctgctta tgtttggatg
     4081 tcagtttctg ccccccgtct ttgggctgac ccctctgggg atgcaggtgg ccggtatttt
     4141 catcggcacg atcctgctgt ggatgttcgt ttcggtcacc tggcccagta tcctgtgcat
     4201 catcgcgctg atcctgtccc cgctgtacac gtattccagc ggcctgtcgg gctctatggg
     4261 gggctggatc acttcctttg tgcttttcag ctccatggtg acctataccc tgggccagac
     4321 cggcttcctc aagcgctgcg cggtctggtt cgtcacccgg ccctgggcgc agaagaaccc
     4381 ctggctgttt ttggggctgc tgtttctggc tcccctggtc atcggttcct ttatgtctcc
     4441 catcccggcc tttatcgtct gcctgcccat tgcagagcag atcttcaagg aattgaagta
     4501 cgagaagggc gaccgtttcc ctcagatcgt cgtgctgggc atcctgtttt ttgcctccct
     4561 gtccactgct gccaccccga tcgcccacac ggtcaccacg atggcactct ccctgtatga
     4621 gaacgacgcc ggggtcccgg tgaatttcgt ctcctatacc atcttcggcg tagcggcatg
     4681 cctggtcact ttcgcggcgt ccatggtgct gaccaaattc gtctgtcgtc cagggacgga
     4741 gcgcctgagc agtctggaca gctctgtgct gaagtctgag accactccga tgagccgcca
     4801 ggagaaatat acgctgctcg tcttcgtgct ggtggtggcc atgtggctgc tgcccgggat
     4861 cattgcgcct gtcctgcccg aggtggcctc cgccatctcc tcgctgggga cgcccacccc
     4921 ggccatgatc ggagcgtgcc tgctgtgcgt cctccatgtg gaggggaagc ctctgatgaa
     4981 cttcaatgag gccgtggcca agggggtgcc ctggggtgct gtgttcatgg tggcggccac
     5041 cagcgtgctg ggcagcgccc tgacccatga agaagcgggc atcaccgctg tggtcagcgg
     5101 agcgctctcc cccatcatcg gcagtatgtc ccccattatc ttcgtgctgt ttatcgccct
     5161 ggccacggtc atgatcacca actttgcctc caacacggtc accgtcactc tgatgtacag
     5221 catctcgctg ccgctggtgt atggcggagc tatttccggg gtcgatcctg ccgcgttgac
     5281 ctgcgtgatc ggcgcctgtg cctgcgtggc ctgcgcgact ccgccctcca cggcccacgc
     5341 cgccattgcg gccgggtccg gatggctgaa tacggatacg atgctcaaat atgggctctt
     5401 gtggtccgtc acagcggccg tgatctttgc ggtggtgggc tacccgatcg ccgcggccat
     5461 catgtgacag agggaaagga gagctgctta tggaacgaca gtttgatgtt attgtggtcg
     5521 gcggcggtac cgccggtttt gcagccgctg tgacggccgc cagagggggc gcaaagacgc
     5581 tgctcatcga ggaaaaggcg tatttgggcg ggaccgccac cggagcgcag gtgagcatgt
     5641 tcatgggttt tgccgatggt gagccggaca ggcctcagca gggcatcatc aaggacgtga
     5701 tggacggcct cgccgctgcg ggcgggacct ccggcatcga gacgatctat ctatgcggac
     5761 gcagagacct ggatatccca gtcatcccct atgagtccga gatactcaaa gatctgatct
     5821 tcgatctggt ggaccaggcg ggcgtggagc tgctcctcca tacccgggtc atcggcgcgc
     5881 aggtagagga cggcgtcatc accgcactga ccatccataa cgagcagggg gtccaaactg
     5941 tgagcggcaa agtggtcatt gacgcctcct tccatgggag cgtggcggtc agtgccggct
     6001 gccgctggga gataggggac gaaaaaggag tccttcagcc cgggaccttg atgtacaaaa
     6061 tggccggtgt ggacatagcg cggtatgagc aggtcagcca gccggagcgg gagcgtctgg
     6121 cacagaaggg gatcgaagaa gggtgccttt atgtcaataa cctgttggca cgccccctcc
     6181 catccggaac gatctactcc aatatgagcc gaattcggat cgatcctctg gatgcggctc
     6241 agtggagccg ggcggagatg gaggcccggc gtcaggtaag gaggatcagc cgcttcttta
     6301 ttgaaaacgt gccgggattt gagaaggcct acctcatctc tacaggagat ttcaccggcc
     6361 tgcgggactc ccgaaggatc caggggaaat atgtcctcac gggtgaggac gtggtagagg
     6421 ggaagctctt tgcagaccct gtagtcagaa gctcctaccc tgtggatatc catgacaccg
     6481 acggtgtctc cagcaccatc gtcaagccca agacgggcgt gttctatatc ccttaccact
     6541 gtctggtcac caacgagatc tccaacctcc tgctggcggg gcggtgtatt tccactgatt
     6601 atgcggccca tgcctgcatc cgtgtcatga taacctgtat gcgcctggga gaggcggcag
     6661 gtctggctgc ggctgagagc gtgcacctgg ggacagcgcc caatgccctg gacggcagga
     6721 gcatctcaaa gcagctgggc ttttgaaaaa ttggatctgg ggctgccgca gggatacctg
     6781 cggcggcccc ccgtattttg atatttggca aaggagaaag ttttgatgaa aacagtgatg
     6841 ctgcgcaatg tcgaggtggg ctgcggaaag cccaaggtga tcgtccccat cgtggccgct
     6901 acggcccagg gcatcctgga gaaggcccgg gagttccgtg cccacgccct ggacgtggtg
     6961 gagtggcggg tggacttcta tgaggatgtc ttccagctgc ctaaggtgct gagcacactg
     7021 aaggggctgc gggagatcct gggggagacc cccatcctgt tcaccttccg caccaggaag
     7081 gaggggggcg agaaggagat cgacatggag gcctatacgg cgctgaacac cgcggtggcc
     7141 cagtcgggag acgcggacgc cgtggatgtg gaggtcttct ccggggacga ggtggtccgc
     7201 cggaacattc aggccatcca cgccgcgggg aaagcggtgg tgggctccaa ccacgacttc
     7261 ggtggcacgc cggacaaggc tgacctgctc ttccgcctgc ggaagatgca ggagctgggg
     7321 gcggacattc cgaagatcgc ggtgatgccc cggtgcccgg gagatgtcat tgccctgctg
     7381 gacgccaccc aggagatgag cgggaagtac gccgaccggc ccatcatcac catgtccatg
     7441 ggggccgggg tggtcagccg cctgtgcggg gagtacttcg gctcggccat gaccttcggc
     7501 gcggtgggcc aggtctccgc ccccgggcag atccccgtgg aggagctgaa cgccgccatg
     7561 gatatcctcc accgggccct ggccccccgg agctgagggg gagggggata aatgagccgt
     7621 caagtcaccg gccgcacggg actgctttgc ctgctgggca gcccggtggc ccacagcatc
     7681 tcccccgcga tgcacaa
//
LOCUS       DBMDSJ010000028         7642 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2932_length_7642_cov_1.973611, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000028 DBMDSJ010000000
VERSION     DBMDSJ010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_7642_cov_1.973611"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="ACZPAQ_01360"
     CDS             <1..347
                     /locus_tag="ACZPAQ_01360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021588763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR0902653.1"
                     /translation="AKAGHKLDALRRCSKASFCVIDQDQVVPPEYTTYFRSVIAFGRT
                     RILEDEAEKQAAIWLLAEKYCPGDSPEHRQEAIRREAGGLCLVSLSIEHMTGKQAIEL
                     VREDRRQADLPL"
     gene            complement(534..2420)
                     /locus_tag="ACZPAQ_01365"
     CDS             complement(534..2420)
                     /locus_tag="ACZPAQ_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR0902654.1"
                     /translation="MKIRRFCSGLLAAVLLLGLMVLPAGAAAASTVSTEEAIQVINAL
                     GIMVGDESGEFHLNRRVTRAEFITMAVNATGTGDQVGEASTSPYPDVPRTHWAAGYVQ
                     AGVQAGLISGYLDGTFRPSNQITLAEGATIVLKLLGYTAEDFSGAYPTGQLAMYRNLK
                     LDRGVTAQKSTDVLTRQDTLYLFYNLLSTNTKEGTPYINKLGYSLNAAGEVDRVALVN
                     GVMEGPVVAAGSWQQSVPFDVDSARVYRDGAASSAKSIQNNDIVYWSESMQTLWVYTD
                     RVAGTIQELSPTPSNPTSVKVAGQTYEIETASAAYELSDLGSYQVGDTVTLLLGRNGG
                     VAAVAAASASQDGSKIGIVTNLSRGTYSGSGANSSYTADTVTILATDGRTYSYQWTTD
                     YFKLGNLVQVVISSEDGSVSLKRLSETGKLSGKVSSDASKLGSYPFADGVEILDTYEG
                     TGVRIYPERLAGLNVTTDMVRYYTLNGAGEITKLILKDATGDMHSYGIMTDVVDSSPE
                     GGVSVIVSYTMDLAGEIVSLPGMSVKYGVKKGPVVVMGNVQAPDKIRQLTEVEAARVS
                     GNTVTAGNRTYLMSDNVLVYEYRGGDYYLSSLDRVKDGGYELSAWYDRSEKDGGRIRV
                     IVAK"
     gene            complement(2436..3665)
                     /locus_tag="ACZPAQ_01370"
     CDS             complement(2436..3665)
                     /locus_tag="ACZPAQ_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0902655.1"
                     /translation="MNFGQSFRLALKSLMTSKMRALLTMLGIIIGVGAVIVITSLGNG
                     MQNMMNEQFEKLGANLVQVQLFMYGGDSRSVDPDDMYELVDKYPQYISGVTPYVSAQL
                     AVRQGSEDFERTSIYGVGETFLNERGATMSGQNLGKGRFISYIDAARYQNVCVIGAYL
                     EEEAFQGDALGKQLSIGGEHFTVIGVLEKNSDMSEGSGDDIIYLPYTTALRLSGSADA
                     SIYMFTSTSKDTAATAKGIIENRLYKTFQSSDYYFVMTSAEMMDAMNAMMGTMMAVLV
                     AIAAISLLVGGIGIMNIMLVSVTERTREIGIRKSLGAKRKDIRGQFIIEAGTTSAIGG
                     VLGILVGIGLASLAGSLIGGMMTSSMGGGSTFSAVPNLSSVAVAFGVSVGIGILFGYL
                     PAKKAAALNPIDALRYE"
     gene            complement(3662..4447)
                     /locus_tag="ACZPAQ_01375"
     CDS             complement(3662..4447)
                     /locus_tag="ACZPAQ_01375"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0902656.1"
                     /translation="MEHLIELKNVYKIYQMGEETVHALDGVSLTIDQGEFVAIVGSSG
                     SGKSTAMNIIGCLDVPTSGTYHLGGIDVSTMDDDQQAEIRNKMLGFIFQQYNLIPKLN
                     VLENVELPLLYAGVDASERKERAMASLQRVGLADKCRNLPSQLSGGQQQRVSIARALA
                     GSPSVILADEPTGALDSRTGREVLGFLKKLNREGDTVVLITHDNSIAVKADRIVRLQD
                     GKIIYDGDAHDPRAVVRPEEMEQDEPAAGGFSDASPAEQEAGA"
     gene            complement(4452..6092)
                     /locus_tag="ACZPAQ_01380"
     CDS             complement(4452..6092)
                     /locus_tag="ACZPAQ_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR0902657.1"
                     /translation="MEATQVKTEEAVQAPGKKEPPKLKVPKKKKKWLKRLIIAVVVLA
                     AIFLIFIRPMMSAGQQFVAGTYLPETAQRQDLRISVSSTGTITPIDSYKVSALVNGEI
                     VEAPFEEGDWVEKGALLYRFDAEDAQNAVQQAQLSVAQAELGYRTAQESVNPYSTASG
                     IVQKLYVSVGDPVAVGDPIADVGDNTTMTVEIPFHSADAAGLYVGQTGTLTIEGTMET
                     VTGTIESISGADEVGAGGALIRQVKFRVSNPGGLTGTTAATAVVGSVACAGSGTFSQS
                     TGQTVVAQTAGEITALHVTSGSPVSYGTVLATIGGTAAETTLENARIGVENAKLALDS
                     RLQALENYEITAPISGTVIEKNFKAGDNVDSSAVTSAGGNLAVLYDMSTLTFEMKVNE
                     LDINNIQVGQEVEITADALEGMTFKGYVDKININGTTANGRTTYPVTVVVEGTPEELK
                     PGMNVSAEVLVEDAGNVLCVPVEAVQRGNTVLVAGQGALDESGNLVDPTKLEERPVTL
                     GRSSSEYIEILDGLEEGETVYVQNIASNAMAMMMGVGG"
     gene            complement(6107..6715)
                     /locus_tag="ACZPAQ_01385"
     CDS             complement(6107..6715)
                     /locus_tag="ACZPAQ_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR0902658.1"
                     /translation="MPSKTFFHLPAEKRERLLLAAEEEFARVPYAEASINRMIRAAGI
                     PRGSFYMYFRDKEELFHYLLKRYQDGLLQRLERVLEEQGGDLLGALPALFDDLRAQRK
                     HWAGLARMLSRNSDMRLGLRLSPLDRPDLGEWLERRVDRDRLDLRGEEDLMDIFRILI
                     YISATAMTQAIFEEDSGPARARLCAQLDILRRGTAAPSATKT"
     gene            7181..7534
                     /locus_tag="ACZPAQ_01390"
     CDS             7181..7534
                     /locus_tag="ACZPAQ_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0902659.1"
                     /translation="MEELGYALRLARERRGLTQTQVMNLTGINNKTLSGYENNIAEPD
                     LQTLTTLLRLYRVSADRLLHLPVSAQELPADEVRLLTLYRAFPEEQRQQLMLVLETLS
                     RSAPKPKPAPKPRQP"
BASE COUNT         1338 a         2478 c         2271 g         1555 t
ORIGIN      
        1 gtgccaaggc agggcacaag ctggacgccc tgcgtcggtg cagcaaggcg tccttctgcg
       61 tcatcgatca ggatcaggtg gtccccccgg agtacaccac ctactttcgc agcgtcatcg
      121 ccttcggccg gacccgtatt ttggaggacg aggcggagaa acaagctgcc atttggctcc
      181 tggcggagaa atactgcccc ggcgacagcc cggagcatcg ccaggaggcc attcgccggg
      241 aggccggcgg actgtgcctt gtgtcgctct ccatcgagca catgaccggc aagcaggcca
      301 tcgagctggt tcgggaagac cgccggcagg ctgatctccc tttgtgaccc ccgatcccct
      361 tttgtgttct ggggctgaat tttccttttt tccaccctgt cccctgcggg gggcggtaac
      421 acacaaaagg ctcttgagca gaaacaagcg gatgccccgc tggggcatcc gcttgttacg
      481 gtcccgaaga gggcgccccg cacagcgggg cgccctcctt atcttatggg agcttatttt
      541 gccacgatga ctcggatacg gccgccgtcc ttctcggagc ggtcatacca ggcggacagc
      601 tcgtaaccgc cgtccttcac gcggtccagg gaggacaggt agtagtcgcc gcccctgtac
      661 tcatagacca gcacgttgtc ggacatgagg taggtccggt tgccggccgt gacagtgttg
      721 ccgctgacgc gggcagcctc cacctcggtc agctgccgga tcttatcggg ggcctggaca
      781 ttgcccatca ccaccacagg gcccttcttc acgccgtact tgacgctcat tccgggcagg
      841 gatacgatct cgcccgccag atccatggtg taactcacga tgacgctgac gccgccctcc
      901 gggctgctgt ccaccacatc agtcatgatg ccgtagctgt gcatatcgcc ggtggcgtcc
      961 ttcaggatca gctttgtgat ctcacccgca ccgttcaggg tgtagtagcg caccatatcg
     1021 gtggtcacgt tcagccccgc cagacgctcg ggatagatcc ttacgccggt gccctcatag
     1081 gtgtccagga tctccacgcc gtctgcgaag ggataactgc ccagtttgga ggcgtcggag
     1141 gacaccttgc cggagagctt gccggtctcg ctcagccgct tgagggacac ggagccgtcc
     1201 tcactgctga tgaccacctg gaccaggttg cccagcttga agtagtcggt ggtccactga
     1261 tagctgtatg tgcggccgtc ggtggccagg atggtcacag tgtcagcggt gtaggaggag
     1321 ttggccccgg agccggagta ggtgccgcgg ctcaggttag tgacgatgcc gatcttgctg
     1381 ccgtcctgag aggcggaggc ggcggccaca gcggccacgc cgccgttccg gcccagcagc
     1441 agggtgacgg tgtcccccac ctgatagctg cccaggtcgg acagctcgta ggcggcggag
     1501 gctgtctcaa tctcataggt ctggcctgcc accttcacgg atgtggggtt ggagggggtg
     1561 gggctcagtt cctggatcgt gccggccacc cggtcggtgt acacccacag ggtctgcatg
     1621 gactcactcc agtagacgat gtcgttgttc tggatggact tggcggagga ggcggcgccg
     1681 tcccggtaca cccgggcgct gtccacatcg aaggggacgg actgctgcca gctgccggcc
     1741 gccaccaccg ggccctccat cacgccgttg accagggcca cccggtccac ctcaccggcg
     1801 gcgttgagtg agtagcccag cttgttaata tagggggtgc cctccttggt gttggtggag
     1861 agcaggttat aaaacagata cagggtatcc tgccgggtca gcacgtcggt ggacttctgg
     1921 gcggtgacac cccggtccag ctttaggttg cggtacatgg ccagctggcc ggtgggatag
     1981 gccccagaga agtcctctgc ggtgtagccc agcagcttga ggacgatggt agcgccctcc
     2041 gccagggtga tctggttgga ggggcggaag gtcccatcca gatagccgga gatcagcccg
     2101 gcctgcaccc ccgcctggac atagccggcg gcccagtggg tccggggcac gtcggggtag
     2161 ggcgaggtgg atgcctcccc cacctgatcc ccggtgccgg tggcgttgac cgccatggtg
     2221 ataaactcgg cccgggtgac ccggcggttc aggtggaact caccgctctc atcgcccacc
     2281 atgatgccca gggcgttgat gacctggatg gcctcctctg tggagactgt ggacgcggca
     2341 gccgctcctg ccggcaggac catcaggccc agcagcagca ctgccgccag caggccggag
     2401 caaaatcgtc tgattttcat ctgtgcttct cctccttatt catagcgcag ggcgtcgatg
     2461 gggttcaggg cggccgcctt ttttgccggc agatagccga acaggatgcc gatgcccacg
     2521 gacacgccga aagccacggc caccgagctc aggttaggca ccgcactgaa ggtgctcccg
     2581 ccgcccatac tgctggtcat catgcctcca atgagggaac cggccaaact ggccagaccg
     2641 atccccacca ggatgcccag caccccgccg atggctgagg tggtgccggc ctcaatgatg
     2701 aactgcccgc ggatatcctt ccgcttggcg cccagggact tgcggatgcc gatctcccgg
     2761 gtgcgctcgg taacggagac cagcatgata ttcataatgc cgatgccgcc caccagcaga
     2821 gagatggcgg cgatggccac cagcaccgcc atcatggtgc ccatcatggc gttcatggcg
     2881 tccatcatct cggcggaggt catgacaaag tagtagtcgc tgctctggaa ggtcttgtac
     2941 agccggttct cgatgatgcc cttggccgtt gcagctgtgt ccttgctggt gctggtgaac
     3001 atgtatatgc tggcgtccgc gctcccgctc aggcgcaggg cggtggtgta ggggaggtat
     3061 atgatgtcgt ccccgctccc ctcgctcatg tcgctgttct tctccagcac accgataacg
     3121 gtaaagtgct ccccgccgat ggacagctgc ttgcccaggg cgtcgccctg gaaggcctct
     3181 tcctccagat aagcgccgat gacgcatacg ttctggtagc gggcagcgtc gatgtaggag
     3241 ataaatcggc ctttgcccag gttctggccg ctcatggtgg cccccctctc gttgaggaag
     3301 gtctcaccca caccataaat gctggtgcgc tcaaaatcct cgctcccctg ccgcaccgcc
     3361 agctgagcgg agacataggg ggtcacgccg gagatgtact ggggatattt atccaccagt
     3421 tcatacatat cgtctgggtc caccgaacgg gagtcccccc cgtacataaa caattggacc
     3481 tggaccagat tggcgcccag cttctcaaac tgctcgttca tcatattctg catgccgttg
     3541 cccaggctgg tgataacgat gacggcaccc acgccgatga tgatgcccag catggtgagc
     3601 agagcccgca tcttgctggt catcagggac ttcagggcca ggcggaagga ctgtccaaag
     3661 ttcatgcgcc cgcctcctgt tccgccgggg aggcgtccga aaatccgcct gccgccggct
     3721 cgtcctgctc catctcctcg ggccgcacca cggccctggg gtcatgggca tcgccgtcgt
     3781 agatgatctt tccgtcctgg aggcggacga tgcggtccgc cttcaccgcg atggagttgt
     3841 catgggtgat gaggaccaca gtgtccccct cccggttcag ctttttcaga aagcccagca
     3901 cctcccgacc ggtgcgggag tccagtgcgc cggtgggctc gtcagccagg atgaccgacg
     3961 ggctgcccgc cagggcccgg gcgatggaca cccgctgctg ctggccgccg gagagctggg
     4021 agggcaggtt cctgcacttg tccgccagcc ctacccgctg gagggaggcc atggctcgct
     4081 ccttccgctc cgaggcgtcc accccggcat agagcagggg gagctccacg ttttccagca
     4141 cgttcagctt ggggatcagg ttatactgct ggaagatgaa gcccagcatt ttgttgcgga
     4201 tctcggcctg ctggtcgtcg tccatggtag acacgtcgat cccgcccaga tgataggtcc
     4261 ctgaggtagg cacgtccaga cagccgatga tattcatggc cgtggacttt cccgagccgg
     4321 aggagcccac gatggccacg aactccccct ggtcgatggt caggctgacg ccgtccaagg
     4381 catggacggt ctcctctccc atctggtaga tcttgtagac gttctttaat tcgatcaggt
     4441 gttccatacg ctcagccacc cacgcccatc atcatggcca tagcgttgga ggcgatattc
     4501 tgaacataga ccgtctcgcc ctcctccagg ccgtccagga tctcgatgta ctcgctgctg
     4561 ctgcggccca gggtgacagg gcgctcctcc agcttggtgg ggtccaccag attgccgctc
     4621 tcatccaggg cgccctgacc ggccaccagc acggtgttgc cccgctggac cgcctctacc
     4681 ggcacgcaca gcacattgcc cgcgtcctcc accagcacct cggcagacac gttcatgccc
     4741 ggcttcagct cctctggtgt gccctccacc accacggtga ccgggtaggt ggtgcggcca
     4801 ttggccgtgg tgccgttgat gttgatcttg tccacatagc ccttgaaggt cataccctcc
     4861 agagcgtcgg cggtgatctc tacctcctgg cccacctgaa tgttgttgat gtccagctca
     4921 ttcaccttca tttcaaaggt cagggtggac atgtcataca gcactgccag gttccctccc
     4981 gcggaggtca ccgcggagct gtccacgttg tcgccggcct tgaagttttt ctcgataacg
     5041 gtgccggaga tgggggcggt gatctcatag ttctccaggg cctggagccg gctgtccagg
     5101 gccagcttgg cgttctccac gccgatgcgg gcgttctcca gggtggtctc cgcggcagta
     5161 ccgccgatgg tggccagcac agtgccgtag gacaccggac ttccggaggt gacatggagg
     5221 gcggtgatct cccccgctgt ctgtgccacc acggtctggc cggtgctttg gctgaaggtg
     5281 ccgcttccgg cgcaggccac gctgcccacc acagcggtgg cggcggtggt gccggtcagt
     5341 ccgccgggat tgctcacccg gaactttacc tgacggatca aggcgcctcc ggcacccacc
     5401 tcgtcggcgc cggagatgct ctcgatggtg ccggtcactg tctccatcgt cccttcaatg
     5461 gtcagtgtgc ccgtctggcc cacgtacagc cccgcggcgt ctgcgctgtg gaaggggatc
     5521 tccactgtca tggtggtgtt gtcccccacg tccgcgatgg ggtcgcccac cgccaccggg
     5581 tcgcccacac tgacatagag cttctgcacg atgccgctgg cagtggagta ggggttcacg
     5641 ctctcctgag cggtgcggta acccagctcc gcctgcgcta cgctcagctg ggcctgctgc
     5701 accgcgttct gggcatcctc tgcgtcaaaa cggtagagca gagcgccctt ctctacccag
     5761 tcgccctcct caaagggagc ctccacgatc tccccgttga ccagggcgga gaccttgtag
     5821 gagtcgatgg gcgtgatggt cccggtgctg gacacggaga tcctcaggtc ctgccgctgg
     5881 gctgtctcag gcagataggt gcccgccacg aactgctggc cggcgctcat catgggccgg
     5941 ataaaaatca ggaaaatggc cgccagcact accaccgcga taatcagtcg cttgagccat
     6001 tttttcttct ttttgggcac cttaagcttg ggcggttcct ttttgcccgg tgcctggacc
     6061 gcctcttccg tttttacctg tgttgcttcc ataccttgaa tctcctttat gtcttggtcg
     6121 cggacggggc tgctgtcccc cgtctcaaaa tgtccagctg ggcgcacagc cgcgcccggg
     6181 ccggaccgct gtcctcttca aagatcgcct gtgtcatggc agtggccgag atgtatatca
     6241 ggatccggaa gatgtccatc aagtcctctt ccccccgcag gtccagcctg tcccggtcca
     6301 cccgccgctc cagccactcc cccaggtccg gccgatccag cgggctcagc cgcaggccca
     6361 ggcgcatgtc cgagttccgg ctgagcatcc tggccagccc ggcccagtgc ttccgctggg
     6421 cccgcagatc atcgaacagg gcgggcagcg ccccgagcag atcgcctccc tgctcctcca
     6481 gcacccgctc cagccgctgg agcagcccgt cctgataccg cttcaggagg tagtggaaca
     6541 gctcctcctt gtcacgaaag tacatgtaaa agctgcctcg ggggatcccc gctgcccgga
     6601 tcatccgatt gatagacgcc tcagcatagg gcacacgggc aaactcctcc tcagcggcca
     6661 acagcagccg ctcccgcttc tccgcgggca ggtggaagaa ggtcttgctg ggcattcccg
     6721 tcccgcctcc tcacatgaca agttgtcaca cagtatagtc ccccttcatt tttctgtcaa
     6781 tcgcccccct ccaggtttca aaaaatgttc acaatccttc tccgccgtac gggctgcagc
     6841 ggacggatat tccgtccccc agccccagtc tctgtcagag gccccatatg cttttctttt
     6901 ttaccatatc agagaatctt taagtacagt acataagaaa aattaaaatt ttcttaaacc
     6961 ggctcctccg ctgcatctgc ctgagtcctg actctacacc ttcccccagg gggaaggtca
     7021 agcaaaatct gtggctggcc cacattttgt ccactggata agccttttca ttttaattcc
     7081 tattttttaa gaagtcaata atattttctt ttttactaag aataaccaga gggatcgtcc
     7141 tgttctgcat taaaataggg gcaaaaggag cgtcagagct atggaagaac tcggatacgc
     7201 cctgcggctg gcgcgggagc gcaggggctt gacccagacc caggtgatga acctcaccgg
     7261 catcaacaac aaaacgctct ccggctatga gaacaacatc gcggagccgg accttcagac
     7321 cctgaccacc ctgctgcggc tctaccgggt gtcggcggac cggctgctcc acctgcccgt
     7381 ctcggctcag gagcttcccg ccgatgaggt caggctcctt accctgtacc gtgcctttcc
     7441 agaggagcag cggcagcagc tgatgctggt gctggagacc ctgtccagat ccgcccctaa
     7501 gcccaagccc gcccctaagc ccaggcagcc gtgaccggaa tctaggcttt tccctggaag
     7561 cagagcaccc cccggagcca agggcccggc cgcataaggg tatcatttaa aaccgtccag
     7621 ggttcagtaa gttgggacac at
//
LOCUS       DBMDSJ010000029         7535 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_2982_length_7535_cov_1.779661, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000029 DBMDSJ010000000
VERSION     DBMDSJ010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7535
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2982_length_7535_cov_1.779661"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(216..887)
                     /locus_tag="ACZPAQ_01395"
     CDS             complement(216..887)
                     /locus_tag="ACZPAQ_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012753.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR0902660.1"
                     /translation="MSGLDLIITITDRSKCELFINWFRGRDIPLVLTALGQGTATTEI
                     LDCLGLEASEKSVLFCLAPHSRCMVRRAARDLWLDVPGNGVLMTVPVSSIGGTSVKEY
                     LTQNQEGEEPMEREIAHELILVIANQGHTDQVMEAARGAGATGGTTIHAKGTGMELAK
                     KFFGVSIASERELVFILTRSQDKKNIMKAIMAQAGIQTPAQAMVFSVPVSDIAGLRHL
                     DQAES"
     gene            complement(928..2442)
                     /locus_tag="ACZPAQ_01400"
     CDS             complement(928..2442)
                     /locus_tag="ACZPAQ_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIR0902661.1"
                     /translation="MGQSKTILLEKLREALSSVLPIVLIVLVLCFSIAPIPTATLMTF
                     LIGAVLLIGGMGLFTLGADQAMTPIGERVGAAMTRSRKLWIVVSVSFLIGVIVTVSEP
                     DLQVLATQVPGVPNPVLIGAVAVGVGIFLVIALLRILFRVPLNRLLVGFYLVVFTLAA
                     FIPGDFLAIAFDSGGVTTGPMTVPFIMALGLGVSSIRSDADASQDSFGLVALCSIGPI
                     LAVMVLSLLYPASGAYTPVSIPEVPDSRALWKLFEEGLPEYIAEVALCLAPIAAFFVV
                     FQVVSLKLKKKKVLKIVVGILYTYIGLVLFLTGVNVGFMPAGNYLGYQIATLPYRWVL
                     IPIGMLMGWFIVQAEPAVHVLNKQVEEITSGAIPGRAMSTSLSIGVAMSIGLSMVRVL
                     TGISIFWLLIPGYLAAITMSFFTPKIFTAIAFDSGGVASGPMTATFLLPFAMGACDAI
                     GGSIITDAFGVVAMVAMTPLITIQMLGLLYQYRLKKNGTSGEQTAPADDDIIEL"
     gene            complement(2773..4104)
                     /locus_tag="ACZPAQ_01405"
     CDS             complement(2773..4104)
                     /locus_tag="ACZPAQ_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HIR0902662.1"
                     /translation="MEKHSAVRDSWGSKWGFILACIGSAVGMGNIWLFPARVSQYGGA
                     TFLIPYLIFVVIIGSTGVIGEMAFGRATRSGPIGAFGYATKLRFGHERPGRLLGLIPV
                     LGSLALAIGYSVVTGWIFKYTADALTGTLSRMSGLDAFAAHFNATAAGNVPWQLVGMA
                     VTIAILAFGIGRGIELANKVMTPLFYVLFVGLAIYLFTLPASGAGYQYIFVLDPKGLL
                     DPVVWIYALGQAFFSLSVAGNGTLIYGSYLSRSSNVPSDARMVAVFDTVAALLAALVI
                     IPAMAVAGQQLDQSGPGLMFIYLPNVLSTIPGGWLILILFFAAVIFAALTSLVNLFEA
                     PTATLQELFHLKRPAAAAITGGAGILVGLVIAPIVDPWMNICSIYICPLGALLAAVMF
                     FWICGRQFVEEQVNLARTRPLGPWFYPLGKYLFCGVTLAVLILGSVMGGIG"
     gene            complement(4569..4751)
                     /locus_tag="ACZPAQ_01410"
     CDS             complement(4569..4751)
                     /locus_tag="ACZPAQ_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR0902663.1"
                     /translation="MNATVNSNCIGCGLCVSICPEVFEIGDDGLAQPIQEVTETLEKT
                     ASQARESCPVNAIDLE"
     gene            complement(4899..5774)
                     /locus_tag="ACZPAQ_01415"
     CDS             complement(4899..5774)
                     /locus_tag="ACZPAQ_01415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR0902664.1"
                     /translation="MHFSQSFAAKLTAGLLAVTGMIAPAFAVSGTVDAEGGLRLRNAS
                     STDAAVLETIPDGSQIEVSGITESGWYQVSFQDTQGYVSTDYVVLSETVELPTVAEPV
                     YGKVTEGPLNVRSAPSTDSEKVDSFSTGKVLTILETLDGWYKVEEGYVSADYVTIIDA
                     AEAASSGSASEVVDLAMQYLGYPYCYGGSSPSGFDCSGFVRYVYSQFGCSLNRTASAQ
                     MSNGTSVSMSELQPGDLVFFLKSGSGASRASHVGIYIGGGQFIHASTSSTGVIISDMD
                     SAYYTTGFVGGRRIL"
     gene            complement(5828..6118)
                     /locus_tag="ACZPAQ_01420"
     CDS             complement(5828..6118)
                     /locus_tag="ACZPAQ_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902665.1"
                     /translation="MASEKIPLSYKGHPLRRKDNLIYYGTMAEKYIIMLQILDTKKVD
                     DLDVATKVAVQLQLTDPDLKSRDRVVKKTEKDSLYTAMDVATVWLDRALSTK"
     gene            complement(6313..7230)
                     /gene="glsA"
                     /locus_tag="ACZPAQ_01425"
     CDS             complement(6313..7230)
                     /gene="glsA"
                     /locus_tag="ACZPAQ_01425"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIR0902666.1"
                     /translation="MDALLEKLVEENRPFTREGRVAGYIPELAKADRDELGICVVSSD
                     GRISSAGEYRRTFTIQSIIKPILLLLALMDNGEDTVRARVGVEATGKPFDAINYTDQA
                     LLSEHLNPMVNMGAILLCTLIHGTSYSQRLERLLELTRRLAGNPSLSVDEAVYRSEKQ
                     TGSRNRAMAFLLESCGLLHDDPEEVLDCYFRACSIRVDCRDLAHIACTFAGRGRRSPS
                     GERLFPARYAHYVNAVLMTCGMYNGSGDFAVKVGLPAKSGVGGGIMAVSPARMGIGIY
                     SPGLDEKGNSLAGIRLLEQLGRRLSLSIF"
     gene            complement(7239..>7535)
                     /locus_tag="ACZPAQ_01430"
     CDS             complement(7239..>7535)
                     /locus_tag="ACZPAQ_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902667.1"
                     /translation="LCSTCSLQIDGDTAAAVAQGWQREGLKMLQQGEKMSGSEDFAEI
                     AARVPSTFFVVGGGTAQDGCGVSLHNPKIRFREEALIYGAAAYASGAAMWLELH"
BASE COUNT         1630 a         2180 c         2442 g         1283 t
ORIGIN      
        1 cccacggctt tggcctggcg gggatggaag agatcgcccg ccgccggggc ggctccctgg
       61 aggcaaaggc ggagggcggg cgcttccagc tgacggtctg cctgcccctg gatgggggaa
      121 actgagcctc tggggacagc ggagacagaa ggcggggaca gagcaagccc cccggcgccg
      181 cggcgccgga gggcttgcta tttgtctggt tcggttcagg actctgcttg gtccaaatga
      241 cgcaggccgg cgatgtcgct caccggaacg gagaagacca tagcctgggc gggcgtctgg
      301 atgccggcct gggccatgat ggccttcatg atgttcttct tgtcctggga gcgggtcagg
      361 ataaagacca actcccgctc agaggcgata gagacgccga aaaacttttt ggccagttcc
      421 ataccggtcc ccttggcgtg gatggtggtc ccgccagtgg cgcccgcgcc gcgggcggcc
      481 tccatgacct ggtccgtatg cccctgattg gcgatcacca agatcagctc atgggcgatc
      541 tctctctcca tgggttcctc cccctcctga ttctgggtca gatattcctt gacggacgtg
      601 ccgccgatgc tggacacggg caccgtcatc agtacgccgt tgcccggcac gtccagccac
      661 aggtcccggg ccgccctgcg caccatacac cgggaatggg gggcaaggca gaacaggacc
      721 gacttttccg aggcctccag cccgagacag tccaggatct ccgtggtggc ggtcccctgt
      781 cccagcgcgg tgagaaccag cgggatgtca cggccgcgga accagttgat aaacaactca
      841 cacttggacc ggtcagtgat ggtgatgata agatccaatc cgctcatagc ggtcactcct
      901 cctcgcgtcg tgggggcggc agcgccctca gagctcgatg atgtcatcat ccgccggggc
      961 ggtctgctct ccgctggtgc cattcttctt caggcggtac tgatagagca gtcccagcat
     1021 ctggatggtg atcagcggcg tcatggccac catggccacc acgccgaagg cgtcggtgat
     1081 gatgcttccg ccgatggcgt cgcaggctcc catggcgaag gggagcagaa aggtggcggt
     1141 catagggccg gaggctacgc cgccggagtc gaaggcaatg gcggtgaaga tcttcggcgt
     1201 gaaaaaggac atggtgatag cggccaggta gccggggatc agcagccaga agatggagat
     1261 cccggtgagc acccgcacca tggacagtcc aatcgacatc gccacgccga tggacagact
     1321 ggtgctcatg gcccggccgg ggatggcacc ggaggtgatc tcctccacct gcttgttgag
     1381 cacgtggacc gccggctccg cctggacgat gaaccagccc atcagcatgc cgattgggat
     1441 caggacccag cgatagggca gggtggcgat ctgatagccc agatagttgc cggcgggcat
     1501 gaagcccaca ttgacgccgg tgaggaacag taccagccca atataagtat ataaaatacc
     1561 gactacgatt ttcaagacct ttttcttttt taatttcagg gataccacct gaaacacgac
     1621 aaaaaaggcg gcgatggggg ccagacacag ggccacctct gcgatgtact cggggagacc
     1681 ctcctcaaac agcttccaga gggcacggct gtccggaacc tctgggatgg agacaggggt
     1741 gtaggcccca gaggcggggt agagcaggga gaggaccatc acggccagga tggggccgat
     1801 ggagcacaga gccaccaggc cgaagctgtc ctgagaggcg tcggcgtcgc tgcggatgga
     1861 ggagacgccc aggcccaggg ccatgatgaa ggggacggtc atggggccgg tggtgacgcc
     1921 tccagagtca aaggcgatgg cgagaaagtc cccggggata aaggcggcaa gggtgaacac
     1981 gaccagatag aagcccacca acaggcggtt cagggggacc cggaacagga tgcgcagcag
     2041 agcgatgaca aggaagatcc ccacccctac ggccaccgcc ccgatgagca ccgggttggg
     2101 cacgccgggg acctgggtgg ccaggacctg gaggtcgggc tcggacacgg tgacgatgac
     2161 tccaatgagg aagcttaccg acaccacgat ccacagcttg cgggagcggg tcatggccgc
     2221 gcctacccgc tcgccaatgg gggtcatggc ctgatcggcc cccagtgtga acagccccat
     2281 cccaccgatc agcagcaccg ccccgatgag gaaagtcatt agggtggcgg tggggatggg
     2341 agcgatggaa aagcacagga ccaaaacgat cagcacgatg gggagtacgg aggagagggc
     2401 ctcccgcagc ttttccagaa ggatggtctt gctctggccc atctgatccc tccaattttg
     2461 catatgttcc cggagcaaaa tcatcacctc gatagagaat atcgtcccaa ttcctttgtg
     2521 ggcgccaatc tccctgtggg gccgggagca gaaaggggaa atttcggccc aggggtacaa
     2581 agtggaactg agcaaaaata taaattattt tatgcgcttt gataacagtt taccatggtt
     2641 ttgcggctgg ctcaaccaaa aagggagata tcttaataca atcttaatgt ttggggcaaa
     2701 ataggcagaa aacggccgga cccgttagaa cgggtccggc cgttaccgga acagtggaga
     2761 gggatgcagc agtcagccga tgccgcccat cacggagccc agaatgagca ctgccagggt
     2821 gaccccgcag aacaggtact ttcccagggg atagaaccag gggcctaagg gcctggtccg
     2881 ggccaggttc acctgctcct ccacgaactg cctgccgcag atccagaaga acatgaccgc
     2941 cgccagcagc gcacccaggg gacagatgta gatggagcaa atgttcatcc aggggtccac
     3001 gatgggggcg atgaccagcc caaccaggat gcctgctccc ccggtgatgg ctgcggcggc
     3061 ggggcgcttc aggtggaaca gctcctggag agtggcggta ggcgcctcga acaggttgac
     3121 cagagaggtg agggccgcga agatcaccgc agcaaagaaa aggatcagga tcagccagcc
     3181 gccggggatg gtgctgagca cgttgggcag atagatgaac atgaggccgg ggccgctctg
     3241 gtccagctgc tggccggcca ccgccatggc ggggatgatg accagggcgg ccagcagagc
     3301 ggccacagtg tcaaagacgg ccaccatcct ggcgtcagag ggaacattgg agctgcggct
     3361 gaggtaggag ccgtagatca gggtgccgtt tcccgctacc gaaagggaaa agaaggcctg
     3421 ccccagggca tagatccaca ccactgggtc cagcagcccc ttggggtcca ggacgaagat
     3481 gtactgatag cccgctccgg aagcgggcag ggtgaacaga tagatggcca gcccgacgaa
     3541 gaggacatag aacagggggg tcatgacctt gttggccagt tcgatcccac ggccaatgcc
     3601 gaaagccaga atggcgatgg tcaccgccat gccgacgagc tgccagggaa catttccagc
     3661 agcagtggca ttgaagtggg cggcgaaagc gtccaggccg gacatgcggg acagggtgcc
     3721 ggtgagagcg tcggcggtgt acttgaagat ccagccggtg acgaccgagt agccgatcgc
     3781 caaggccagg gagcccagca ccgggatcag gcccagcagg cggccgggcc gctcatggcc
     3841 gaagcgcagc ttggtggcat agccgaaggc accgatgggg ccggagcggg tggcccgtcc
     3901 aaaggccatc tccccgatca ccccggtaga gccgatgatg accacaaaaa tgaggtacgg
     3961 gatgagaaag gtggccccgc cgtactggga gacacgggcg gggaagagcc agatgttgcc
     4021 catccccacc gcagagccga tgcaggccag gatgaacccc catttagacc cccaggagtc
     4081 gcggacagcg ctatgttttt ccatggaaag acgccccttt tcaaaaaata ccgccggaac
     4141 acgagaaaac cggcaggggc catgcgggcc atgccgggta acactttttt aattatagca
     4201 cacacttgga aaaaaggcaa agaaaacctg gtcacgttcg ttttctcacc actgcagagg
     4261 cgctgggctg ccgcagggag gaaaattttc cctagactcc ggctgttaag atgtctccaa
     4321 ggggatacgt cgtgtgggtg ggacctcgcc ctatacttgt cctatctaca cccgccgccg
     4381 tggtaccagc atatactgac tgtcgagaaa cagcatccca cgccacagat gcggggaaca
     4441 aaaatagcca cggccgccgg aagaattggc agccgtggcc gttatgaaag atacaacaag
     4501 gcgagcatac aacagagccc tccggacggc ggaaggaccg ctgccggagg gaaagggggc
     4561 ggaagtgctc actccaggtc gatggcattg acagggcagg attccctggc ctgggaggcg
     4621 gtcttctcca gggtctcagt gacctcctga atgggctgag ccagtccgtc gtctccgatc
     4681 tcaaagacct ccggacagat gctgacgcac agaccgcagc cgatgcagtt ggaattgaca
     4741 gtagcgttca tgatgtgctc ctttcctccc cggccagcgg atgccgcggg gcgtatctga
     4801 gcacagtatg cccgaaaagc agaaaaaata cacaaacaga aaaaggacag gcaggccccg
     4861 caggggctgc ctgtccggta taacgtaggg gaagaaaatc agaggatccg gcggccgccc
     4921 acgaagccgg tggtgtagta ggcactgtcc atgtcgctga tgatgacgcc ggtgctggag
     4981 gtggaggcgt ggatgaactg gcccccgccg atgtagatac ccacgtggga ggcacggctg
     5041 gcaccgctgc ccgacttcag gaagaagacc agatcgccgg gctggagctc actcatagag
     5101 acggaggtgc cgttgctcat ctgagcggaa gcagtgcggt tgagggagca gccaaactga
     5161 gagtagacat agcggacaaa gccggagcag tcaaaaccgc tgggggagga gccgccgtaa
     5221 cagtagggat agcccagata ctgcatggcc agatccacca cctcggaggc ggagccggaa
     5281 gaggcggcct cggccgcgtc gataatggtc acatagtccg cggacacata gccctcttcc
     5341 accttgtacc agccgtccag ggtctccaga atggtgagga ccttgccagt agagaaggag
     5401 tccaccttct cactgtcggt gctgggggcg gagcggacat tcaaggggcc ctccgtgacc
     5461 ttgccgtaga cgggctccgc cacagtaggc agctctacag tctcagacag cactacataa
     5521 tcggtggaga catagccctg agtatcctgg aaggagacct ggtaccagcc gctttcggta
     5581 ataccagaca cctcaatctg ggagccgtcg gggatggtct ccagaacggc ggcgtcggtg
     5641 ctggaagcgt tgcgcagacg aagaccgccc tcggcgtcca ctgtgccgga cacagcaaaa
     5701 gccggagcga tcataccggt gacagccagc aggccggcgg tcagcttggc agcaaaagac
     5761 tgagaaaagt gcatggcgga agtcctcctg atcgtcatat ctctctttct ggagcgcggg
     5821 cggctcttta cttcgtgctc agtgcccggt ccagccagac cgtggccacg tccattgcgg
     5881 tgtataagct gtccttctct gtctttttga ccacccggtc ccggctcttc aggtcgggat
     5941 cggtcaactg gagctgaacg gccacctttg tggccacatc cagatcgtcc accttcttag
     6001 tgtccaggat ctggagcatg atgatgtact tttcggccat cgtgccgtag tagatgaggt
     6061 tgtctttccg gcgcagggga tggcccttat aggaaagagg gatcttttca gacgccatgg
     6121 tctgcctcct taaggaaatt ttaatgttgt aaccgaattg taacacattt gaaacccttt
     6181 gtcaaatact ctcatctaat ttttttctgg gaaatggagg aaaggcggcg gcagagggga
     6241 tggaaagcgg aaaaacggcc tccggagctg cctctggaga ccgtttttcg cctgaatggc
     6301 ggagatgcgg ggtcagaata tgctcaggct gagccggcgg cccagctgtt ccagcagccg
     6361 gatgccagcc agactgtttc ccttttcgtc cagcccgggg gaatagatgc cgatgcccat
     6421 acgggcaggg gagaccgcca tgatgccgcc ccccacgccg cttttggctg gcagtcccac
     6481 tttgacggcg aagtcccccg agccgttgta catgccgcag gtcatcagca cggcgttgac
     6541 atagtgggca tatcgggcgg ggaacagccg ctcgccggag ggggagcggc ggccccggcc
     6601 ggcgaaggtg caggcgatat gggccaggtc ccggcagtcc accctgatgg agcaggcccg
     6661 gaaataacag tccagcacct cctccgggtc gtcatgaagc agtccgcagc tctccagcag
     6721 aaaggccatg gcccggttac ggctgccggt ctgtttttcc gagcggtaga ccgcctcgtc
     6781 caccgacagg ctgggatttc cagccagccg gcgggtcagc tccagcagcc gctccagccg
     6841 ctgggaatag ctcgtcccgt ggatcagggt gcacagcagg atggctccca tgttgaccat
     6901 gggattgaga tgctcgctga gcagggcctg gtccgtatag ttgatggcgt caaagggttt
     6961 gcctgtggcc tccactccca cccgggcgcg gaccgtgtcc tccccgttgt ccatcagcgc
     7021 caggaggagc aggatgggct tgatgatgct ttgaatggtg aaggtccgcc tgtactcccc
     7081 cgcggagctg atacgtccgt cgctggacac cacgcagatg cccaactcgt cccggtccgc
     7141 cttggccagc tccgggatgt agccagccac ccggccctcc cgggtgaagg ggcgattttc
     7201 ctccaccagc ttttccagca gcgcgtccat gacgtccctc agtgcagctc cagccacatg
     7261 gctgcgccgg aggcgtaggc ggcggcaccg tagatgagcg cctcctcccg gaagcggatc
     7321 ttgggattgt ggaggctgac accgcagccg tcctgggcgg tgccgcctcc caccacgaag
     7381 aaggtggagg ggacgcgggc ggcgatctcg gcgaagtcct cggaaccgct cattttctca
     7441 ccctgctgga gcattttcag tccctcccgc tgccacccct gggcgacggc ggcggcagta
     7501 tccccgtcga tctggaggga gcaggtggag cagag
//
LOCUS       DBMDSJ010000030         7346 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3065_length_7346_cov_2.104624, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000030 DBMDSJ010000000
VERSION     DBMDSJ010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3065_length_7346_cov_2.104624"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="ACZPAQ_01435"
     CDS             complement(<1..1040)
                     /locus_tag="ACZPAQ_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR0902668.1"
                     /translation="MLDIKFIRENPDAVRENIKKKFQDAKLPLVDQVLALDEENRAAV
                     SEANELRANRNALSKQVGMLMGQAKKDPSKLEEAEAVKAQVKANADRLAELESKETEL
                     AQQIRHIMLQIPNIIDPSVSIGPDDSYNVEVQRFGEPVVPDFEIPYHTQIMESFNGID
                     MDAAGRVSGNGFYYLMGDIARLHEAVLAYARDFMINKGFTFCIPPFMIHGNVVEGVMS
                     QTEMDAMMYKIEGEDLYLIGTSEHSMIGKFIDQIIPEEFLPQTLTSYSPCFRKEKGAH
                     GIEERGVYRIHQFEKQEMVVVCKPEDSMKWYEQMWRYSVELFRSLDIPVRQLECCSGD
                     LADLKVKSCDIEA"
     gene            complement(1168..1758)
                     /locus_tag="ACZPAQ_01440"
     CDS             complement(1168..1758)
                     /locus_tag="ACZPAQ_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902669.1"
                     /translation="MADPQKPAQNAPQAGGPPKEERPGQQRRRRQSVVLYITILFAAA
                     FALLLMTYAMDQRRSNETLGDLTSSVSDLKDSLSGMQTAQSALEENGALKNEIQDLQE
                     QIDTLLGTIDSLTAAGEQQGQVLERTKEAMDYFWQIDEAYVRGRYSLCRDLIAVLEDT
                     SSGEAPLKSYLPQESYTDNGRFSPADRYQEIWDALY"
     gene            complement(1751..2644)
                     /locus_tag="ACZPAQ_01445"
     CDS             complement(1751..2644)
                     /locus_tag="ACZPAQ_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR0902670.1"
                     /translation="MAKRTKELGLGRGLNALLGDPDLQAQGEGSVSLPISQVQPGLNQ
                     PRKRFDQDALADLAESIRVHGIIQPLTVRRLSSGYYQIIAGERRWRAAKLAGLAEVPA
                     VIIEADDRKVMELGLIENLQREDLNPAEEARGYQVLMEEYGLTQEQVADRMGKSRPAI
                     TNTLRLLALPEDLLKLVEEEQLSAGHARALLGAPTAELQRQAAKKVIADGLSVRQTEA
                     LVKALQREKKAPPKQAGDSLALYLGEAEKNLSGRLGRKVHIAHRGKKGRIELEYYNSQ
                     DLELLLEALERLNAGEVQGHG"
     gene            complement(2736..3509)
                     /locus_tag="ACZPAQ_01450"
     CDS             complement(2736..3509)
                     /locus_tag="ACZPAQ_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR0902671.1"
                     /translation="MAKIIAIVNQKGGVGKTTTTVNLTAALAALGKRVLLCDFDPQAN
                     ATSGMGVDKNTASPNVYDVLINGADPRRGVVTTHYGDVLPSNKALAGAGVEMIALPDR
                     EHLLRKALEVLSPGYDFIFVDCPPSLELLTVNALCAADSLLVPVQCEYYALEGLSDLL
                     STVRLVKRSLNPRLVLEGVLLTMFDSRTNLSLQVAEEVKRHFPGQVYATVIPRNVRLS
                     EAPSHGKPVTAYDPYSRGAEAYRLLAEEIAAKHPTAAPV"
     gene            complement(3789..4595)
                     /locus_tag="ACZPAQ_01455"
     CDS             complement(3789..4595)
                     /locus_tag="ACZPAQ_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR0902672.1"
                     /translation="MQLLRKKGLLDSPRVLFLPVDAISPNPNQPRRVFGTQELEELAG
                     SIQALGLLQPLTVRRRADGWELVAGERRLRAAKLAGLEEVPCISLQIDDQRSSLLALV
                     ENLQRKDLDFWEEALALDRLISVYHLSQEEAARRIGKSQSAVANKLRLLKLPEDTLRA
                     LRDGGCTERHARALLRLEDPSLRQKALEQILSRQLTVAQTDALVDQLLSPPPRTRTKR
                     TFVVKDVRLFLNTLTRSMALMRSAGVDARCDRQDKDGEILLTIRIPQAIR"
     gene            4893..6137
                     /locus_tag="ACZPAQ_01460"
     CDS             4893..6137
                     /locus_tag="ACZPAQ_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR0902673.1"
                     /translation="MNKKKLPLAFWIFIGLVVGIAVGLALMMVPNGLDIAKNYILPWG
                     TIFLNLLKFIVVPIVLFSIASGVISMQDIGRVGSVGGKTIVYYMCTTAFAVVLALVLA
                     SAAKGMHIFAAMETSGLAYEPPAGQSLMQTIIDIFPSNPITPLANASMLQVIVISLLV
                     GFGILLAGEKGLVAAQVVDSFNEVSMKIMDLIIKLSPIGVACLICPVIVENGPKILAQ
                     LVAVLAVAYVGYIVHAVVVYSSTVKALGGVSPIAFFKGMAPAMMMAFSSASSVGTLPF
                     TLECSERLGARKEVASFVLPLGATINMDGTAIYQGVCAVFIASCYGIDLTIGQMATIV
                     LTATLASIGTAGVPGAGMVMLAMVLQSVNIPVEGIALVAGIDRIFDMGRTTINITGDA
                     ACAVVVSKMEDRKVARTKSVLG"
     gene            6340..7221
                     /locus_tag="ACZPAQ_01465"
     CDS             6340..7221
                     /locus_tag="ACZPAQ_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0902674.1"
                     /translation="MQGEEFNIQANGVEIHGVRMGQGEPLLLLHGHPETCLMWHKVAP
                     QLAERFTVVATDLRGYGDSGKPEGLPDHSNYSKRTMAEDQIEVMRALGFERFHVMGHD
                     RGARVAYRMALDHPEAVNKLVLLDIVSTYDMYSLSTREFAKALFQWYFFTQEAPLPED
                     MILSSRKQFFRYSLHIARYHSENDTSAEAFPQDVYDEYLRHYNVETIHAICEEYRAGE
                     TVDLALDEEDLRAGRKIRAETLVLWGANGLVERFFRPLECWAKFGDHIQGHNLPCGHF
                     IPEEAPLPMLEAVENFL"
BASE COUNT         1422 a         2118 c         2357 g         1449 t
ORIGIN      
        1 gcctcgatgt cacaggactt taccttcagg tcagccaggt cgccggagca gcactccagc
       61 tggcggaccg ggatatccag ggagcggaac agctctaccg agtagcgcca catctgctcg
      121 taccacttca tggagtcctc cggcttgcac accaccacca tttcctgctt ctcaaactgg
      181 tggatgcggt agacgccccg ctcctcgatg ccgtgggccc ccttctcctt gcggaagcag
      241 ggggagtagg aggtaagggt ctggggcaga aactcctcgg ggatgatctg gtcgatgaac
      301 ttgccgatca tggagtgctc gctggtgccg ataagataga ggtcctcgcc ctcgatcttg
      361 tacatcatgg cgtccatctc ggtctgggac atgacgccct ccaccacgtt gccgtggatc
      421 atgaaggggg gaatacagaa ggtgaagccc ttgttgatca taaagtctcg cgcataggcc
      481 agcaccgctt catggagccg ggcaatatcc cccatgaggt agtagaagcc gttgccggag
      541 acccggccgg cagcgtccat gtcgatgccg ttgaaggact ccatgatctg ggtgtggtag
      601 gggatctcaa agtcggggac cacaggctca ccgaagcgct ggacttccac attgtagctg
      661 tcgtcagggc cgatagagac ggagggatca atgatattgg ggatctgtag catgatgtga
      721 cggatctgct gcgccagttc agtctctttg gactccagct cagccagccg gtcggcgttg
      781 gccttgacct gggccttgac ggcctcggcc tcctccagct tggaggggtc ctttttggcc
      841 tggcccatca gcatacctac ctgcttggac agggcgttcc ggttggctct cagctcattg
      901 gcttcgctga cagcggcccg gttctcctcg tccaatgcca gtacctggtc caccaggggc
      961 agtttggcat cctgaaactt tttcttgata ttttcccgca ctgcatcggg gttttcacga
     1021 ataaatttga tatccagcat ggggatcgca ctcctttttt atcttaacag aatgggatga
     1081 gctcctgtgg ggaaattggg cgaaaataaa aatgtttcac gtgaaacctg gccggcggag
     1141 atgtcctgcc atccggccta aagccggtca gtacagggcg tcccagatct cctgataccg
     1201 gtcagcaggg gagaagcggc cgttatcggt gtagctctcc tggggaagat agctcttcag
     1261 gggggcctct ccggaggagg tgtcctccag taccgcgatc agatcacggc agagggagta
     1321 gcggccccgc acataggcct catcgatctg ccagaagtag tccatggcct ccttggtgcg
     1381 ctccaggacc tgtccctgct gctccccggc ggcggtcagg ctgtcgatgg tccccagcag
     1441 ggtgtcgatc tgttcctgga ggtcttggat ctcatttttc agggcgccgt tctcctccag
     1501 ggcgctctgg gcggtctgca tcccggagag ggagtccttc agatcagaga ctgaggaggt
     1561 caggtccccc agcgtctcat tgctgcggcg ctggtccatg gcgtaggtca tcagcagcag
     1621 ggcaaaggcc gctgcaaaga ggatagtgat gtagagcacc accgactgcc tgcgccgccg
     1681 ctgctggcca ggacgctcct ccttgggagg accgcctgcc tggggggcgt tctgcgccgg
     1741 cttctgggga tcagccatgg ccctgcacct ccccggcgtt cagccgctcc agagcctcca
     1801 gcagaagctc cagatcctgg ctgttgtagt attccaactc gatcctgcct tttttgcctc
     1861 ggtgggcgat gtggaccttc cggcccaacc ggccggagag attcttttcc gcctccccga
     1921 ggtagagggc cagactgtcc cccgcctgct taggcggggc cttcttttcc cgctggaggg
     1981 ccttgaccag tgcctccgtc tggcggacgg agagcccgtc agcaatgacc ttcttggcgg
     2041 cctgccgctg gagctccgcc gtgggggcgc ccagcagggc acgggcgtgg ccggcggaga
     2101 gctgttcctc ctccaccagc ttcaagaggt cctccgggag ggccagcagc cgcagggtat
     2161 tggtgatggc aggccgggat tttcccatcc gatccgccac ctgttcctgg gtgaggccgt
     2221 attcctccat cagcacctga tagccccggg cctcttcggc cgggttcagg tcctctcgct
     2281 ggaggttctc gatcaggccc agctccatca ctttgcggtc atccgcctcg atgatgactg
     2341 ccggcacctc cgccaggccg gccagcttgg cggcccgcca gcggcgctca ccggcgatga
     2401 tctggtagta gccggaggac agacggcgca ctgtcagcgg ctggatgatg ccgtggaccc
     2461 ggatagactc tgccagatcg gccagggcat cctggtcaaa gcgcttccgg ggctgattca
     2521 ggccgggctg tacctgggaa atggggaggg agacggagcc ctctccctgc gcttgcaggt
     2581 ccgggtcgcc cagcagggcg ttcagccccc ggcccaggcc cagctctttg gttcgctttg
     2641 ccatagtagc tccttcctta taaaaataac agtggagatc aaaattcagc cgtcggggcc
     2701 agacttacgg ccccggcgaa aaagcggggg agaactcaga caggggccgc cgtggggtgc
     2761 ttggctgcga tctcctccgc cagcagccgg taggcctccg ccccccggga gtaggggtcg
     2821 taggcggtga cgggcttccc gtggctgggg gcctcggata agcgcacatt gcgggggatg
     2881 accgtggcgt agacctgacc gggaaagtga cgtttaactt cctccgccac ttgaagagag
     2941 agattggtgc ggctgtcaaa catggtgagc agcacgccct ctaacaccag ccggggattc
     3001 agtgagcgtt tgaccaagcg gacggtagag agcagatcgc tcagtccctc cagggcgtag
     3061 tactcacact ggaccgggac cagcagggag tcagcggcgc acagggcgtt cactgtcagc
     3121 agctccagag agggggggca gtccacaaag atgaagtcgt agccgggaga gaggacctcc
     3181 agggcctttc ggagcagatg ctcccggtcc ggcagggcga tcatctccac gcccgctccg
     3241 gccagcgcct tgttggaggg aagcacgtcc ccgtagtgtg tggtgactac gccgcggcgg
     3301 ggatcggccc cattgatgag cacgtcgtag acatttgggg aggcggtgtt cttgtccact
     3361 cccataccag aggtggcgtt ggcttggggg tcgaagtcgc acagcagtac ccgctttccc
     3421 agcgcggcca gggcggcggt gaggttgacg gtggtagtgg tcttgcccac gcctcccttt
     3481 tgattgacga tagcgatgat cttggccatg gggcgcctcc tgagtcgaaa tggcggacgg
     3541 gtgtacagca gacttccgca gagctttatt atatcaaccg agggaacgca tttcaactct
     3601 ttttcccgtt tttccggcct ggtagcgaga aaaggcggcg gaccgcacag gggcggcccg
     3661 ccgcaagggg agaggtgcag acagagggag agagccgaga ccgggcctgc ctccaggcga
     3721 tctggcttcg gcaggcgatg gggaggaaag gggagatagg aaaagagaat gtttcacgtg
     3781 aaacattttt accgaatggc ctggggaata cggatggtga gcaggatctc tccatcctta
     3841 tcctggcggt cacagcgggc atccaccccg gcagaccgca ttagggccat gctccgagtc
     3901 agagtgttaa gaaaaaggcg gacatccttc accacaaagg tgcgtttggt tctggtgcgg
     3961 ggaggagggg agagaagctg atctaccaag gcgtctgtct gggccacggt gagctgccgg
     4021 gacaggatct gctccagggc cttctggcgc aggctggggt cctccagccg aagcagggcc
     4081 cgggcatggc gctcagtgca gccgccgtcc cgcagggccc ggagagtgtc ctccggcagc
     4141 ttcagcaggc gcagcttgtt ggccacggcg gactggctct tgccgatgcg ccgggcagcc
     4201 tcctcctggg agagatggta gacagagatc aaccggtcca gggccagggc ctcctcccag
     4261 aagtccaggt ccttgcgctg caagttctct accagagcca gcagggagga gcgctggtcg
     4321 tcgatctgga gagagatgca ggggacctct tccagtcctg ccagcttggc ggcccggagc
     4381 cgccgttccc cggctaccag ctcccagccg tctgcccgac ggcgcacagt caggggctgg
     4441 agcagcccca gagcctggat ggaaccagcc agctcctcca gttcctgggt gccaaagacc
     4501 cggcggggct gattgggatt gggtgagatg gcgtctacag ggaggaacag cacccgggga
     4561 ctgtccagca atcccttctt tcgcagcagc tgcattctgg ataccccctt tgatccttcc
     4621 gctgttataa aacagcttgt cttgatatcc atattttacc ataaaaaaat aaaaagaaag
     4681 ttggattctg tcgcagttgt ggaaaatcca gtggaaaagt tgaaaaaaga agggcttggc
     4741 ccgggaaatt gcagggggag aaaaaggaaa gaaagacctt gtaagtttcc tccatatcat
     4801 atatactgaa agagagcagt cagagaggga gctgtaaaca gatgcggccc aaagtgagga
     4861 aagggctgca tggacggaaa ggagagctgt caatgaacaa gaagaaactt ccccttgcat
     4921 tttggatctt catcgggctg gtggtcggca tcgcagtggg cctggccctg atgatggtgc
     4981 ccaacggact ggacatcgcg aaaaactaca ttctgccctg gggcacgatc ttcctgaacc
     5041 tgctgaaatt catcgtggtg cccatcgtgc tgttttccat cgcctccggt gtcatctcta
     5101 tgcaggacat cggccgggtg ggctcggtgg gaggcaagac gatcgtgtat tacatgtgca
     5161 ccaccgcttt tgccgtggtg ttggccctgg tgctggccag cgcggccaag gggatgcaca
     5221 tctttgcggc catggagacc tccggcctgg cctatgaacc cccggcgggc cagtcgctga
     5281 tgcagaccat catcgacatc ttcccctcca accccatcac gccgctggcc aatgcctcca
     5341 tgctccaggt catcgtcatc tctctcctgg tgggcttcgg catcctgctg gcgggggaaa
     5401 aggggctggt ggccgcccag gtggtggaca gcttcaatga ggtgtccatg aagatcatgg
     5461 acctgatcat caagctctcg cccatcggcg tggcctgcct gatctgcccg gtgatcgtgg
     5521 aaaacgggcc caagatcctg gctcagctgg tggcggtgct ggcggtggcc tatgtgggct
     5581 atatcgtcca cgccgtggtg gtgtactcca gcacggtgaa ggccctgggc ggcgtatccc
     5641 ccatcgcctt ctttaagggg atggcccctg ccatgatgat ggccttctcc tccgcatcct
     5701 cggtgggcac cctgcccttc accctggagt gctccgagcg gctgggggcc cggaaggagg
     5761 tcgcctcctt cgtgctgccc ctgggcgcca ccatcaacat ggacggaact gccatctacc
     5821 agggggtgtg cgccgttttc atcgcatcct gctatgggat cgacctgacc atcggccaga
     5881 tggccaccat cgtgctcacc gccaccttgg cgtctatcgg cactgcgggc gtccccggcg
     5941 ccggcatggt gatgctggcc atggtgctcc agagcgtaaa tatcccggtg gagggcatcg
     6001 cgctggtggc cggcatcgac cggatctttg acatgggccg caccaccatc aacatcacgg
     6061 gcgatgcggc ctgcgcagtg gtggtctcca agatggagga ccgcaaggtg gcccgcacca
     6121 agagtgtttt gggctgatca aagaacagag agcgggcccc ggaccaaatg gtccggggcc
     6181 cgctgctttg tttgaagggc cggtatgcat ggaggccata ggtctatgaa aaaagcaaaa
     6241 tagtccaacg tggggacatc tgctaaaata gggggcagaa gaaacttaaa attgggcaga
     6301 gtgctcaata tagaggaaag ggtgaagaga ccgatgcgga tgcagggaga ggaattcaac
     6361 atccaggcca acggtgtgga aatccacggg gtcaggatgg ggcaggggga gccgctcctc
     6421 ctcctccacg ggcacccgga gacctgtctg atgtggcaca aggtggcgcc ccagctggcg
     6481 gagcgcttca cagtggtggc caccgacctg cggggctacg gggacagcgg aaagccggag
     6541 gggctgcctg accacagcaa ctactccaag cggactatgg cggaggacca aatcgaggtg
     6601 atgcgggccc tgggctttga acgcttccac gtcatggggc atgatcgggg ggcccgggtg
     6661 gcctaccgca tggcgctgga ccatccggag gcggtaaata aactggtgct gctggatatc
     6721 gtgtccacct atgacatgta cagcctctcc acccgggagt ttgccaaggc gctgttccag
     6781 tggtatttct ttacccagga ggccccgctg ccggaggaca tgatcctgtc cagccggaag
     6841 cagtttttcc gctattccct gcacattgcc cggtatcact cggagaacga cacctcagcc
     6901 gaggcatttc cccaggacgt ctacgacgag tatctgaggc actacaacgt ggaaaccatc
     6961 cacgccatct gtgaggagta ccgggcgggg gagacggtgg atctggcgct ggacgaggag
     7021 gatttgcggg ccgggcggaa gatccgggcc gagaccctgg tgttgtgggg ggcaaacgga
     7081 ctggtggagc ggttcttccg ccccctggag tgctgggcca aatttggaga ccacattcag
     7141 ggacacaacc tgccctgcgg ccattttatc cctgaggagg cccctctccc catgctggag
     7201 gcggtggaaa acttcctttg acaacagacg caagaaaaag acagcgcccc gccgtggaga
     7261 gagtccgcgg cggggcgctg tctggttaaa agaaaacatg aaaatgtcac atgaatacat
     7321 gagaaagtcg tattggaagt taaaat
//
LOCUS       DBMDSJ010000031         7346 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3066_length_7346_cov_1.578882, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000031 DBMDSJ010000000
VERSION     DBMDSJ010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_7346_cov_1.578882"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="ACZPAQ_01470"
     CDS             <1..1354
                     /locus_tag="ACZPAQ_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR0902675.1"
                     /translation="YLTRRLVDVSQEVIIREEDCGTRDGIEVSEIEEYGQVIETFAER
                     LHGRYPAEDIVDPATGELLFSRDTMLRNEDAKVLEAHGIHSAKIRSVLTCEARSGVCA
                     RCYGINLAIGEPVGAGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGGDITQGLPRVEE
                     LFEARKPKKMAQLAEISGRVSIEETKRNIMCNVTITADDGEVVTYALPYAAGLRVKDG
                     DVVAKGDQLTEGALSPHEVLRIRGLSACHNYLIREVQKVYRQQGVDTNDKHIEVIIRQ
                     MMRKVRVEDAGDSDLLSGATIDIIEFWDAEKAVRTRMEAGEKNEMGEDLRLPTCTRLL
                     MGITKASLATDSFLSAASFQETTKVLTEAAIKGKVDHLLGLKENVIIGKLIPAGSGLA
                     QYRKDDPIDENGNLITTETAGAVEAEPLEEPEEEGFFAEEEPEVEAAADTQEDSGTLV
                     "
     gene            1530..1664
                     /locus_tag="ACZPAQ_01475"
     CDS             1530..1664
                     /locus_tag="ACZPAQ_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR0902676.1"
                     /translation="MTEEEAAGYHRTKRRGSTMIEWLMALVMAGIVALWLYQNGKDGK
                     "
     gene            1724..2539
                     /locus_tag="ACZPAQ_01480"
     CDS             1724..2539
                     /locus_tag="ACZPAQ_01480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HIR0902677.1"
                     /translation="MDLLVTLDQNYLGPLRVLLGSLFLNDPGEEFHIYMIEEGLTGAQ
                     VEELSQLCRAHHSQLHPLEVPDGLFTDAPVVRYYSRAMYYRLLAAELLPRELDRVLYL
                     DPDILVIRPVRPLYETQLGDSLLAAASHSGLTGMSDYVNKIRLSNYEAESYYNSGVLL
                     MNLPQMREEMRPADIFAYAREYEERLILPDQDILNGLYGTRILGVDDSVWNYDARRYD
                     RYWLGSHGEKDMDWVMDHTVFLHFCGKNKPWSRGYEGHFSALYKHYQRLLGEV"
     gene            complement(2782..4053)
                     /locus_tag="ACZPAQ_01485"
     CDS             complement(2782..4053)
                     /locus_tag="ACZPAQ_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR0902678.1"
                     /translation="MKRVSALLLTCTLGLSALSGCSASAAPQPLSAQPVTISEEEMMR
                     YDLETPAVWSALSEFGLDLLKTARQEGEGTLLSPLSAALALSMAANGADGETLDQFRE
                     VLGDGASLEARNVSCSALMKLYGGLGGSTECSIANSLWTDPEGRMQEDFVGRCSGIFD
                     AQVFQEDLSAPVIVPALNSWVSDHTAGMIPQIIQRPFGEDAAALMVNALYLKNTWAAK
                     FDPSDTVTRDFTRADGVAEPLDFLQHFSRSLTYLQSGSDEGVLLPYDDGRLAFFALMP
                     RQSSGVPDFERWLSSLTGEGLSALLSGREETFFLRLSLPKFEQEWSGELKDALAALGL
                     TDAFDPDLADFSLLGDDPRGYYLSQVIHAARIEVNEEGTEAASATVVAAASGAAPPQE
                     GVTLIFDRPFLYGIVDLQNGVPLFLGTFEGL"
     gene            4611..4850
                     /locus_tag="ACZPAQ_01490"
     CDS             4611..4850
                     /locus_tag="ACZPAQ_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HIR0902679.1"
                     /translation="MLEELKAGSRVVGAKQTRRALKDGRAKRVYMAKDADPRLLQPLV
                     QEAVRQRVPLIQVDTMRQLGEACGISVGAAIVAAV"
     gene            4949..5371
                     /gene="rpsL"
                     /locus_tag="ACZPAQ_01495"
     CDS             4949..5371
                     /gene="rpsL"
                     /locus_tag="ACZPAQ_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR0902680.1"
                     /translation="MPTFNQLVRKGREQVTYKSTSPALQHGLNTLKSRQTDLHAPQKR
                     GVCTAVRTSTPKKPNSALRKIARVKLTNGYEVTAYIPGVGHNLQEHSVVMIRGGRVKD
                     LPGVRYHIIRGSLDTQGVNGRMQARSKYGAKRPKAGKK"
     gene            5602..6072
                     /gene="rpsG"
                     /locus_tag="ACZPAQ_01500"
     CDS             5602..6072
                     /gene="rpsG"
                     /locus_tag="ACZPAQ_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR0902681.1"
                     /translation="MPRRGNVPKREVLPDPLYNSILVTKLTNSIMLDGKKGVAQKVVY
                     GAFDIIKEKTDKDPMEVYTQALENIMPSLEVKARRVGGATYQVPIEVRPERRQTLGLR
                     WLTTYARARSEKTMKERLAGEIMDAANSLGNAVKKREDTHKMAESNKAFAHYRW"
     gene            6087..>7346
                     /gene="fusA"
                     /locus_tag="ACZPAQ_01505"
     CDS             6087..>7346
                     /gene="fusA"
                     /locus_tag="ACZPAQ_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR0902682.1"
                     /translation="MPRKVTLENTRNIGIMAHIDAGKTTTTERILYYTGVNYKIGETH
                     DGTATMDWMAQEQERGITITSAATTCYWQGTKNQFPQTRLNIIDTPGHVDFTVEVERS
                     LRVLDGSVTVMCAKGGVEPQSETVWRQADNYKVPRMIYVNKMDIMGADFYNVLNMIHE
                     RLKCNAVPVQLPIGKEDTFKGIIDLVEMDADIYYDEMGKDMRVEEIPADMMELAQEYR
                     TKLVDACADIDDEIMELALEEKPIPQEKLRAALRKGTIENLVVPVTCGTSYRNKGVQK
                     LLDAIVDFMPSPLDIPAIKGVNPDTEEEDERPADDNAPFSALAFKIATDPFVGRLSFI
                     RVYSGVLNTGTTVLNSTKKQKERIGRILQMHANHREDIECVYSGDIAAVVGLKNSTTG
                     DTLCDDKHPIILESMEFPEPVIRVAIEP"
BASE COUNT         1565 a         2193 c         2341 g         1247 t
ORIGIN      
        1 ttacctgacc cgccgtctgg tggacgtgtc ccaggaggtc atcatccggg aggaggactg
       61 cggcacccgg gacggcatcg aggtcagcga gatcgaggag tacggccagg tcatcgagac
      121 ctttgccgag cgcctccacg gccgctaccc cgccgaggac attgtggacc ccgccaccgg
      181 cgagctcctg ttcagccggg acaccatgct ccgcaatgag gacgccaagg tgctggaggc
      241 ccacggcatc cactccgcca agatccgctc cgtcctcacc tgtgaggcac gcagcggcgt
      301 gtgcgccagg tgctacggca tcaacctggc catcggtgag cctgtgggcg ccggcgaggc
      361 ggtaggcatc atcgccgcac agtccatcgg agagcccgga acccagctga ccatgcgtac
      421 cttccacacc ggcggcgtgg ccggcggcga catcactcag ggtctgcccc gagtggagga
      481 gctgttcgag gcccgtaagc ccaagaagat ggcccagctg gccgagatca gcggccgggt
      541 gtccattgaa gagaccaagc gcaacattat gtgcaacgtg accatcaccg ccgacgacgg
      601 cgaggtggtg acctatgccc tgccctatgc cgccggcctg cgcgtcaagg acggcgacgt
      661 ggtggccaag ggcgaccagc tcaccgaggg tgccctgtcc ccccacgagg tgctgcgcat
      721 ccgcgggctg tccgcctgtc acaactatct gatccgcgag gtccagaaag tgtaccgtca
      781 gcagggcgtt gacaccaacg acaagcacat cgaggtcatc atccgccaga tgatgcggaa
      841 ggtccgggtg gaggacgcgg gagattccga cctgctctcc ggcgctacca tcgacatcat
      901 cgagttctgg gacgccgaga aggctgtgag gacccgcatg gaggccgggg aaaagaacga
      961 gatgggcgag gacctgcgcc tgcccacctg cacccgtctg ctcatgggca tcaccaaggc
     1021 atctctggcc accgattcct tcctgtccgc cgcctccttc caggagacca ccaaggtgct
     1081 caccgaggcc gccatcaagg gcaaggtgga ccacctgctg gggctgaagg agaatgtcat
     1141 catcggcaag ctgatccccg ccggttccgg actggcacag taccgcaagg acgaccccat
     1201 cgacgagaac ggcaatctga tcaccacgga gaccgccggc gcagtggagg ccgagccctt
     1261 ggaggagccg gaagaggagg gctttttcgc cgaggaggag cctgaggtgg aggccgctgc
     1321 cgacacccag gaggacagcg gaacgctggt gtaatatcgc tccttcccca acagaacagc
     1381 acgtccagtg agaaagcccc ggcgcatgcg cgccggggct ttttgtcagt ttggttgttt
     1441 cccaccccag ggcggcggct gggcagggca gtcggaacgg gtgaaaggga aataagctct
     1501 gttgaaagtt atgtcaactg aaaaacacct tgacggaaga ggaggcggcg ggctatcata
     1561 gaacgaaaag gagggggagc accatgatag agtggctgat ggcgctggtg atggcgggga
     1621 tcgtggccct ctggctgtat cagaacggaa aggacggaaa ataacggggc cggatgccgg
     1681 tcctgtaccc gctgccaggg aggcggcgga agaggaggaa cgtatggatc tgctggtgac
     1741 attggatcag aattacctgg gaccgctgcg ggtgctgctg ggctccctgt ttctcaatga
     1801 tccaggggag gaatttcata tctatatgat cgaagagggg ctgaccgggg cacaggtgga
     1861 ggagctttcc cagctgtgcc gggcccacca cagccagttg cacccgctgg aggtcccgga
     1921 cggcctgttc acggacgcac cggtggtgcg gtactactcc cgggccatgt actaccgcct
     1981 tctggcggca gagctgctgc cccgggaact ggacagggtg ctctatctgg acccggatat
     2041 cctggtgatc cggccggtgc ggcccctgta tgagacacag ctgggagaca gcctgctggc
     2101 ggcggccagc cacagcgggt tgaccgggat gtctgactat gtaaacaaga tcaggttgtc
     2161 caactatgag gcggagagct actacaactc cggtgtgctg ttgatgaacc ttccccagat
     2221 gcgggaggag atgcgcccgg cagatatttt cgcctacgcc agggagtacg aggagcggct
     2281 gatcctgccg gaccaggaca tcctcaacgg cctgtacggc acccggatcc tgggtgtgga
     2341 cgacagtgtg tggaactatg acgcccggcg atatgaccgc tactggctgg gcagccacgg
     2401 ggagaaggat atggactggg tgatggacca cacggtgttc ctccacttct gcggcaagaa
     2461 caagccctgg tccagagggt atgagggaca tttttccgcc ctctataagc actaccagcg
     2521 cctgctggga gaggtgtaac gcagagaggc tgtcgaaaaa gtagtagcct gccgcacgca
     2581 gggcctgacc gcctgcggcg gacaaacccc acatctgcag cctgagaagt gttttccccg
     2641 gcgtacatgc cgccggggaa aacaggtcag aactggattt gcgcctgcgt gtgcgaactc
     2701 tgggaggctt tttccataag ctgaggggcg cggcctggaa tcaggccgcg ccccgtgatc
     2761 tcgacatagg gccctgacgg atcataggcc ttcaaacgtg cccagaaaca ggggcactcc
     2821 gttctgcagg tccacgatgc catagaggaa ggggcggtcg aagatcaggg tgacgccctc
     2881 ctggggcggg gcggcgccgg atgccgcggc aaccactgtg gcggacgccg cctcggtgcc
     2941 ctcctcattg acttcgatcc tggcggcatg gatcacctgg gagaggtagt accccctggg
     3001 gtcatccccc agcagggaga agtcggccag gtccgggtca aaagcgtccg tcagtccaag
     3061 agcggccagg gcgtctttca gctctcccga ccactcctgc tcaaatttgg gcagggacag
     3121 gcggaggaag aaggtctcct ccctgccgga gaggagggcg gacagcccct ccccggtgag
     3181 ggaggacagc cagcgctcaa agtccgggac gccggaggac tgccggggca tgagggcgaa
     3241 gaaagccagc cgcccgtcgt cataggggag gaggacgcct tcgtcgctgc cgctttggag
     3301 ataggtcaga gagcgggaga agtgctggag gaagtccagg ggctccgcaa ctccgtcggc
     3361 ccgggtgaag tcccgcgtaa cggtgtcgct ggggtcgaat ttggccgccc aggtgttctt
     3421 caggtagagg gcattgacca tcagggcggc ggcgtcctcc ccgaagggcc gctggatgat
     3481 ctgggggatc atgcctgcgg tatggtctga cacccagctg ttgagggcgg ggacgatgac
     3541 tggggcggac aggtcctcct ggaacacctg agcgtcgaaa atgccggagc atctgcctac
     3601 aaagtcttct tgcatacggc cctccgggtc ggtccacagg ctgttggcaa tggagcactc
     3661 cgtggagccc cccagtcctc catagagttt catgagggca gagcaggaga cgttgcgggc
     3721 ctccaggctg gcaccgtccc ccagcacctc ccggaactgg tccaaggtct ccccatcagc
     3781 accattggcg gccatggata gtgccagggc cgcggaaagg ggggatagga gtgttccctc
     3841 cccctcctgg cgggcagttt tcaacaggtc cagcccaaat tcagacaggg cggaccagac
     3901 ggcgggggtc tccaggtcat aacgcatcat ctcctcttcg gagatcgtca ccggctgggc
     3961 agagagaggc tgcggggcgg cagaggcgga gcagcccgac agagcggaca gccccagggt
     4021 gcaggtgagg agcagggcgg agacgcgttt cataagatcc ctccttgttt tttgataggg
     4081 cctgtgtggg acagcgccgt aatggcggga gactctcccg tcttttactc tgttagacgg
     4141 cggaagggga aaaaagttcc ctgggggaga aaaatagagg cgggaggacg cagagcggac
     4201 tgtgggatgc gtgtccttcc cgtccccgtg cagggcggaa catctctgac agatcctacg
     4261 gctgcgactg ggacgggaga aggaaggccc ctcatccgtc gcggcatacg ccgcggcacc
     4321 ttccccccaa agggaaggct ttgggcgggc agagcccttt gggccatcct gcgggtggga
     4381 cgggcaagat gcacaccgct cccggtcccg cagggaacag gaacgaccag gggaagcttt
     4441 ggcccaggga tattaagatg gaattttgag ttttgcatca aaacagaaga aaatttgctc
     4501 ttgacggcgg gcggagacca tggtataatc cgaaattgcg gtggtagggg cattctgccc
     4561 ttttacccga tgaaacgccc ccggcgacgg gggagaggag gggcaccgaa atgctcgaag
     4621 aactgaaagc gggttccagg gtcgtgggag ccaaacagac ccggcgggcc ctgaaggacg
     4681 gacgtgcgaa aagggtctac atggccaagg acgccgaccc caggctcctc cagccgctgg
     4741 tgcaggaggc ggtgcggcag cgcgtccccc tcatccaggt ggacaccatg cgccagctgg
     4801 gagaggcctg cggcatctcc gtgggcgccg ccatcgtggc ggcggtatga ccccatattt
     4861 ttggttttaa cagaataagg tcttcctgat tctgttaaaa tatatattac gccccaatgt
     4921 gggcagaaat cgagaaagga ggaaaaacat gcctactttt aatcagctgg tccggaaggg
     4981 ccgtgagcag gtgacttaca agtccacttc ccccgccctc cagcatggcc tgaacactct
     5041 gaagagccgt cagaccgacc tgcacgctcc ccagaagaga ggtgtctgca ccgcggtgcg
     5101 tacttccacc cccaagaagc ccaactccgc actgcgtaag atcgcccgtg tgaagctgac
     5161 caacggatac gaggtcactg cctatatccc cggtgtcggc cacaacctgc aggagcactc
     5221 cgtggtcatg atccgcggcg gccgtgtcaa ggacctgccc ggtgtgcgtt accacatcat
     5281 ccgcggctct ctggataccc agggcgtcaa cggccggatg caggcccgtt ccaagtacgg
     5341 cgccaagcgg cccaaggccg gcaagaagta attttttgcg gagcaaaaaa ttgctgtatg
     5401 ggcggatatc acccgcccgc cgctcaaatc tgctgagatt tgaggcttga attgcattgt
     5461 gcgttctgcg caaggcaaac cgccttgccg agcgttttcc tatatttttt aatatgggca
     5521 gacctcggac aggcattaca caaactgcaa ggcagtttga gtacctatga tttctaattt
     5581 tataagaagg agggaagcaa agtgcccaga agaggaaatg tacccaagcg ggaggttctg
     5641 cccgatccgc tgtataactc catcctggtt accaagctta ccaacagcat catgctggat
     5701 ggcaagaagg gtgtggccca gaaggtggtc tacggcgcct ttgatatcat caaggagaag
     5761 accgataagg accccatgga ggtctatacc caggctctcg agaacatcat gccctccctg
     5821 gaggtcaagg cccgccgtgt gggcggcgcc acctaccagg tgcctatcga agtgcgtccc
     5881 gagcgccgtc agaccctggg gctgcggtgg ctgaccacct atgcccgggc ccgcagtgag
     5941 aagaccatga aggagcgcct ggccggcgag atcatggacg ccgccaacag cctcggcaac
     6001 gccgtcaaga agcgcgagga cacccacaag atggctgagt ccaacaaggc gttcgctcac
     6061 taccgctggt aataaggaga aacagtatgc ctaggaaagt taccttagag aataccagaa
     6121 acatcggcat catggcccac atcgacgccg gcaagaccac caccaccgag cgcatcctgt
     6181 actacaccgg cgtcaactat aagatcggtg agacccatga cggcaccgcc accatggact
     6241 ggatggctca ggagcaggag cgcggcatca ccatcacctc tgccgccacc acctgctact
     6301 ggcaggggac taagaaccag ttcccccaga cccgtctgaa catcatcgac accccaggcc
     6361 acgtggactt caccgtggag gtggagcgct ccctgcgcgt gctggacggc tctgttaccg
     6421 tcatgtgcgc caagggcggc gtggagcctc agtctgagac tgtctggcgc caggcggata
     6481 actataaagt cccccgcatg atctacgtca ataagatgga catcatgggc gccgatttct
     6541 acaacgtgct caacatgatc cacgagcgcc tgaagtgcaa tgcggtgcct gtccagctgc
     6601 ctatcggcaa ggaggacacc ttcaagggca tcatcgacct ggtggagatg gacgccgaca
     6661 tctactatga tgagatgggc aaggacatgc gggtggagga gatccccgcc gacatgatgg
     6721 agctggccca ggagtaccgc accaagctgg tggacgcctg tgccgacatc gacgacgaga
     6781 tcatggagct ggcgctggaa gagaagccca tcccccagga gaagctgcgt gccgctctgc
     6841 gcaaggggac catcgagaac ctggtagtcc ccgtgacctg cggcacctct taccgcaaca
     6901 agggcgtgca gaagctgctt gacgccatcg tggactttat gccctccccc ctggacatcc
     6961 ccgccatcaa gggcgtgaac cccgacaccg aggaggagga cgagcgtccc gctgacgaca
     7021 acgccccctt ctccgctctg gccttcaaga tcgccaccga ccccttcgtg ggccgtctgt
     7081 ccttcatccg tgtgtattcc ggcgtgctga acaccggcac cacggtgctc aactccacta
     7141 agaagcagaa ggagcgcatc ggccgcatcc tccagatgca cgccaatcac cgggaggaca
     7201 tcgagtgcgt ctactccggc gacattgccg ccgtggtggg cctgaagaac tccaccaccg
     7261 gcgacaccct ctgcgacgac aagcacccca tcatcctgga gtccatggag ttccccgagc
     7321 ccgtcatccg cgtggccatt gagccc
//
LOCUS       DBMDSJ010000032         7338 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3069_length_7338_cov_1.788310, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000032 DBMDSJ010000000
VERSION     DBMDSJ010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_7338_cov_1.788310"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="ACZPAQ_01510"
     CDS             complement(<1..471)
                     /locus_tag="ACZPAQ_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR0902683.1"
                     /translation="MQEPQTQGITVTREQIGSYLSSLGERGRNPSTVSSYRKKLELLY
                     AALPGGRIGRGTLRQWREQLLDQGYSPSTVNTSVSAANSFLEWLGCREFQLTGRLSCG
                     RDAQLELTRSEYLRLLSTARALRQERVYLLIKLFTSTGLPVHELEQVTVEAAGEG"
     assembly_gap    676..685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(815..1657)
                     /locus_tag="ACZPAQ_01515"
     CDS             complement(815..1657)
                     /locus_tag="ACZPAQ_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR0902684.1"
                     /translation="MEPRKITEETLAAFARQLGEEERSPATLEKYLREVRQFAAFLGG
                     REVTRELAAAWREELSARQSPATVNGKLTALDRLLASLGWEDCRVKHLRVQRQLFRDS
                     ARELSREEYARLVETARRLGRGRLSLLMETICATGIRVSEVRYITAEAVREGRTEIAL
                     KGKIRTILLPGKLCRKLEKYARQKKITSGELFLTRSGRPMSRKQIWAEMKGVCRAAGV
                     APSKVFPHNLRHLFARCFYRVSRDVAKLADVLGHSSIETTRIYLISTGAEHARTLDQL
                     RLIS"
     gene            1899..3260
                     /locus_tag="ACZPAQ_01520"
     CDS             1899..3260
                     /locus_tag="ACZPAQ_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0902685.1"
                     /translation="MGVMPENRLLLTMAIPIVISMLVQALYNVVDSVFVAQLSEDALN
                     AVSLAFPVQNLMIAVAVGTGVGINALLSKSLGEGNQAKADRTAMNGLFLSALSFLVFA
                     VIGLTCSRLYFTIQTDIQRIVDYGESYMMICCVCSFGVFFQITLERTLQATGRTLYTM
                     FTQATGAIINIILDPIMIFGLFGFPRMEVAGAALATVTGQIVAAILGLFFNLKKNPDL
                     HLSVRGFRPSRTIIGGIYSVGIPSIVMQSIGSVMVFGMNQILIAFTQTATAVFGVYFK
                     LQSFIFMPVFGLNNGMVPIVAYNYGARKPARIMKTIRLSVCYAVGIMLVGLLIFQFAS
                     PALLRLFQSGESASGDLLRIGVPALRIISYSFLFAGYCIVATAVFQALGHGVLSLVVS
                     VVRQLFVLLPVAFLLSRVGGLDWVWWAFPIAELVAVVLCTVFLKHVYHKEIAPPAGER
                     EAA"
     gene            complement(3360..5441)
                     /locus_tag="ACZPAQ_01525"
     CDS             complement(3360..5441)
                     /locus_tag="ACZPAQ_01525"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR0902686.1"
                     /translation="MDNLFWIGFVGAAIAIAFAWLQSRRVMTFSEGSDKMAKIAASIR
                     EGANAYLKHQYTTVAKVFLVVFLVLLVMAFATGGEMLSQFTPFAFLTGGIWSMLAGFV
                     GMKIATSSNARTAQAASESLNKGLRVAFSSGSVMGFTVVGLGMLDISIWFVILRFVAG
                     IDDPLTLGNIMVMNGMGASFMALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNP
                     ATIADNVGDNVGDVAGMGADLYESYVGSILATFSLGAIAGYGWAGMILPMMLAVCGIV
                     CSIIGSFFVKTKEDATQKSLLTSLRTGTYLAAALSAVAAAPLTYLMLGNWGVYIAILC
                     GLVGGCTIGYFTEYYTSDTYKPTQQLADASETGSATIIIGGISLGLKSTMASILIVAA
                     AVIVSFYAAGGSQSFNLGLYGIGIAAVGMLSTLGITLATDAYGPVADNAGGIAEMSGL
                     PEAVRERTDALDSLGNTTAATGKGFAIGSASLTALALLVSYVSIVQEKTPETLDLSLT
                     NPLMLVGLFIGAMLTFVFSAYTMSAVQTAAQSIVLEVRRQFREIAGIMEYKADPDYAS
                     CVALCTKGALREMVKPALLAIIVPILTGLILGPVGVAGLLGGVSVTGFAMAVFMSNAG
                     GAWDNAKKYIESGHHGGKGSECHKAAVVGDTVGDPFKDTSGPSLNILIKLCSTVSIVF
                     SGLIVAFHLLG"
     gene            complement(5614..7050)
                     /locus_tag="ACZPAQ_01530"
     CDS             complement(5614..7050)
                     /locus_tag="ACZPAQ_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR0902687.1"
                     /translation="MNLKLVSRLVGRVLLVEAFFMLLPLAVALLYQEDPAPFLLTILL
                     MAILGLALSAVPATRHFYAREGFFAVGLIWLVTGVMGALPFYFSGTFPSFVDCLFESV
                     SGFTTTGATILTDIEAQPRGILFWRAFTHWMGGMGVLVLLTALLPSLGIRSHYLTQAE
                     TPGPVFSKLVPKQSQTSKILYGIYCAMTLAEVALLKLAGMPLYDSFIHAFSTAGTGGF
                     SNRNASVGAYGDPVLEMIIAVFALLFSINFAMYFLLLCRRFREVLQSDELRFFLVVVG
                     LSTAVIAFNIRHLYQGPLQCLRYAFFQVSSIISTTGFATADFCLWPQFSQIIFILLML
                     CGACAGSTGGGIKCSRILLAGRAIRREVHRIIHPRSVEIVKLDGKPVDESTLASVLLF
                     ISCYAMITLGASLVVALNDFPFTETLTSVITCISNTGPGLGAVGPAGNFSAFSPLSKL
                     VLSLCMIIGRLEIFPILVMFSRSTWRRV"
     gene            complement(7047..>7338)
                     /locus_tag="ACZPAQ_01535"
     CDS             complement(7047..>7338)
                     /locus_tag="ACZPAQ_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR0902688.1"
                     /translation="VMNALYRIADGAAEAMEFTVTASTRHLGTPLRDLSLKPGILVAV
                     VARGGEIIIPEGSTSIQEGDSVILISRDRRLQDLNDIYDEGEHTFSAPGGMS"
BASE COUNT         1503 a         2125 c         2364 g         1336 t
ORIGIN      
        1 cccctcgccc gccgcctcca ccgtcacctg ttccagctcg tggaccggca atccggtgga
       61 ggtgaacagc ttgatcagca gatagacccg ctcctgtctc agagcccggg cggtggacag
      121 caggcgcagg tattcgctcc gggtcagctc cagctgggca tccctgccgc aggagagccg
      181 ccccgtcagc tggaactccc tgcagcccag ccactccagg aagctgttgg ccgcggacac
      241 gctggtgttg accgtgctgg gggagtatcc ctggtccagc agctgctccc gccactggcg
      301 cagggtgccc cggccgatgc ggccgccggg cagggcggca tacagcagct ccagcttctt
      361 tcggtaggag gatactgtgc tggggttccg ccctcgttcc cccaggctgg acagatagga
      421 gccgatctgc tcccgggtga ccgtgatccc ctgtgtctgg ggctcctgca atgcgttctc
      481 cccttgggag atgcggctct ctttcgttac cccatacgac tccctcccat ttatagacag
      541 aattgttctt ccgtcataga aaatatgcgg gatagagcgg cgttgacagt ttcctccttt
      601 ccaggcagaa gaaaaaagtg tctaaaatat caggattttc tgaaaaaagg cacgacaaat
      661 caggccaaaa agacannnnn nnnnntttgt catgcctgaa tctggaaaaa agattgattt
      721 ctatttcaga aaatggtaaa atataaacta ctccaattat gtattttgaa attttggagc
      781 aattttaatg acagaagaac aattttgtca taagctagga gatcagccgt agctgatcca
      841 gggtccgggc atgctcggcg ccggtggaga tgaggtagat gcgggtggtc tcgatgctgg
      901 agtggcccag cacgtcagcc agctttgcca catcccggct cacccggtag aaacaccggg
      961 caaacaggtg gcgcaggttg tggggaaaga ccttggacgg agcgaccccg gccgcccggc
     1021 acaccccctt catctctgcc cagatctgct tccgggacat gggcctgccg cttctggtga
     1081 ggaacagctc gccggaggtg attttttttt gccgggcgta cttctccagc ttccggcaca
     1141 gcttccccgg cagaaggatg gtgcggatct tccccttcag ggcgatctcc gtccggccct
     1201 cccgcactgc ctccgccgtg atatagcgca cctcgctgac ccggatgccg gtggcacaga
     1261 tggtctccat cagcagggac agccgacccc gccccaggcg gcgggccgtc tccaccagcc
     1321 gggcgtactc ctcccggctg agctcccggg cggagtcccg gaacagctgc cgctgcaccc
     1381 gcaggtgctt cacccggcag tcctcccagc ccagagaagc cagcaggcgg tccagggcag
     1441 tcagcttgcc gttgaccgtg gcgggggact ggcgggcgga cagctcctcc ctccaggcgg
     1501 cggccagctc ccgggtcacc tcacggccgc ccaggaaggc ggcgaactgg cggacctccc
     1561 gcaggtattt ttccagggtg gcggggctgc gctcctcctc tcccagctgt cgggcaaagg
     1621 ccgccagcgt ctcctctgtg atcttccgtg gttccatatg attcctcctg tgtctaaagg
     1681 tctttcctta gtttaccttg acggaggagg attattgctg tctgcactgc aaaaacatct
     1741 tccttgtgcg gccgcagcaa aacagagaat ggaaatcttg acaagcgggg gatgcatcta
     1801 tataataaat atgtttcgca tttggacgaa aacactcgaa tcggatcata aaggaggcag
     1861 tcgggttgag tacagaaaac caggcgcagg aaaacaaaat gggggtcatg ccggaaaacc
     1921 ggctgctgct gaccatggcg atccccattg tcatatccat gctggtccag gcgctttaca
     1981 atgtggtgga cagcgtcttt gtggcacagc tgagcgagga tgcgctgaac gccgtgtcgc
     2041 tggcctttcc ggtgcagaat ctgatgatcg ccgtggcggt gggcaccggt gtgggcatca
     2101 acgccctgct gtccaagagc ctgggcgagg gcaaccaggc caaggcggac cgcaccgcga
     2161 tgaatggact gttcctcagt gccctcagct ttctggtgtt tgcagtcatc ggcctgacct
     2221 gttcccgcct gtacttcacc atccagacgg acatccagcg gatcgtggac tacggcgaga
     2281 gctacatgat gatctgctgt gtgtgctctt tcggcgtgtt cttccagatc acactggagc
     2341 ggaccctcca ggccaccggc cgtacgctct ataccatgtt cacccaggcc accggcgcca
     2401 tcatcaacat catcctggac cccatcatga tcttcggcct gttcggcttc cccaggatgg
     2461 aggtggccgg agcggcgctg gctactgtta ccggacagat cgtggcggcg atcctgggcc
     2521 tgttcttcaa tttaaagaag aatccggacc tccatctgtc ggtgcggggc ttccgaccca
     2581 gccgcaccat catcggaggt atttacagcg tgggcattcc ctccatcgtc atgcagtcca
     2641 tcggctcggt gatggtcttt gggatgaacc agatcctgat cgccttcacc cagacggcca
     2701 ccgccgtctt tggcgtgtac ttcaagctcc agtccttcat cttcatgccc gtctttggat
     2761 tgaacaatgg gatggtgccc atcgtggcct ataactacgg cgcgcggaag cccgcccgga
     2821 tcatgaagac catcaggctc agcgtctgct acgcagtggg catcatgctg gtggggctgc
     2881 tcatcttcca gtttgcctcc cccgcgctgc tgcggctctt ccagtccggg gagagtgcct
     2941 ccggcgattt gctgcgcatc ggagtgccgg cactgcgtat catttcttac agctttttgt
     3001 ttgcaggcta ctgtatcgtg gccactgccg tattccaggc actggggcac ggcgtgctca
     3061 gtctggtggt ctctgttgtc cggcagcttt tcgtactgct tccagtggct ttcctgctct
     3121 ccagggtcgg cgggctggac tgggtctggt gggccttccc catcgcggag ctggtggcgg
     3181 tagtcctgtg tacggtgttc ctgaaacatg tgtatcacaa ggagatcgcc cctccagcag
     3241 gagaacggga ggccgcctga gcagggggga gtccagagga tcagccccgc ttttttctga
     3301 aaataaaaac agtccgtaat tcttctaaga agaattacgg actgtttttg cgttgaagtt
     3361 tagcccagca gatggaaggc cacgatgaga ccggagaaaa cgatggacac ggtggaacac
     3421 agcttgatga ggatgttcag ggaggggccg gaggtgtcct tgaagggatc gcccacggtg
     3481 tctcccacca cggcggcctt gtggcactcg gagcccttgc ccccgtgatg gccggattcg
     3541 atgtacttct tggcgttgtc ccaggcaccg ccggcgttgg acatgaacac ggccatggca
     3601 aagccggtga cggagacgcc gcccagcagg cccgccacac cgacggggcc caggatcagg
     3661 ccggtgagga tggggacgat gatggccagc agggcgggct tcaccatctc ccgcagagcg
     3721 cccttggtgc acagggccac gcaggaggcg tagtcggggt ctgccttgta ctccatgatg
     3781 ccggcgatct cccggaactg gcggcggacc tccagcacga tggactgggc ggcggtctgc
     3841 accgcgctca tggtgtaggc ggagaagacg aaggtgagca tggcgccgat gaacagcccc
     3901 accagcatca agggattggt gagggacagg tccagggtct ccggggtctt ctcctggacg
     3961 atgctcacat aggacaccag cagggccagg gcggtgaggg aggcggagcc gatggcgaag
     4021 cccttgccgg tggctgcggt ggtgttgccc aaggagtcca gagcgtcggt gcgctcccga
     4081 acggcctcag gcaggccgga catctcggcg atgcctccgg cgttgtcagc cacgggaccg
     4141 taggcatcgg tggccagggt gatgcccaga gtggagagca ttcccactgc ggcgatgccg
     4201 atgccgtata ggcccaggtt gaaggactgg ctgccgccgg cggcgtagaa gctgacgatg
     4261 acggccgcgg ccacgatgag gatggaggcc atggtggact tcaaccccag ggagatgccg
     4321 ccgatgatga tggtggcgga gccggtctca gaggcgtcgg ccagctgctg ggtgggcttg
     4381 taggtgtcag aggtgtaata ctcggtgaaa tagccgatgg tgcagccgcc caccagcccg
     4441 cagaggatgg cgatgtagac cccccagttc cccagcatga gataggtcag gggggcggcg
     4501 gccacggcgg agagggccgc agccagataa gttccggtgc ggagggaggt gagcagggac
     4561 ttctgggtgg catcctcctt ggtcttgacg aagaaagagc cgatgatgga gcagacgatg
     4621 ccgcagacgg ccagcatcat gggcaggatc atgcccgccc agccgtaccc ggcgatggcg
     4681 cccagggaga aggtggccag gatagagccc acataggact cgtacaggtc ggcacccatg
     4741 ccggccacat cgcccacgtt gtcgcccacg ttgtccgcga tggtggcggg gttgcggggg
     4801 tcatcctccg ggatgccagc ctccaccttg cccaccagat cggcgcccac gtcggctgcc
     4861 ttggtgtaga tgcccccgcc cacccgggcg aacagggcca tgaaggaggc gcccatgccg
     4921 ttcatcacca tgatgttccc cagggtgagg gggtcgtcga tgccggccac gaagcggagg
     4981 atgacgaacc agatggagat gtccagcatg cccagaccca ccacagtgaa acccatgaca
     5041 gagccggagg agaaggccac ccgcaggccc ttgttcagac tctcgctggc ggcctgagcg
     5101 gtgcgggcgt tggagctggt ggcaattttc atgcccacga agccggccag catggaccag
     5161 atacctccgg tgagaaaggc gaagggggtg aactgggaga gcatctctcc cccggtggca
     5221 aaggccatca ccagcaggac gaggaagacc accagaaaga ccttcgccac agtggtgtac
     5281 tggtgcttca gataggcgtt ggccccctcc cgaatggagg cggcgatctt ggccattttg
     5341 tcactgcctt cggagaaggt catgaccctg cggctttgga gccaggcaaa cgcaatggca
     5401 atggccgcgc ccacgaaacc gatccagaac agattgtcca tcattcaaca cactccttcg
     5461 cgcttggaaa aagggccccc gccagagcgg aggcccttcc atgcaaccgc tgttatggtc
     5521 atattgtacc atactagacg ggctttgcaa tagaaaattt atcgttaagt aaaaaacaaa
     5581 ccgcaggaat ggagaaaaaa cgcagagatc tgcctagacc cggcgccagg tgctgcggct
     5641 gaacatgact aaaattggga agatctccag gcggccgatg atcatgcaca gggagaggac
     5701 cagcttgctc aagggggaga aagcggagaa attgccggcc ggtcccaccg cgcccagtcc
     5761 cgggcctgtg ttgctgatgc aggtgatgac ggaggtaagg gtctcggtaa agggaaaatc
     5821 gttcagggcg accaccaggg aggcacccag ggtgatcatg gcataacagg agataaaaag
     5881 gagcacggag gcgagcgtgg actcgtccac cggcttgccg tccagcttca cgatctccac
     5941 agagcggggg tggatgatgc ggtggacctc ccggcggatg gcccggccag ccagcaggat
     6001 gcgggagcat ttgatgccgc cgccggtgga accggcacag gcgccgcaca gcatcagcag
     6061 gataaagata atttgagaga actgaggcca caggcagaag tctgccgtgg cgaagccggt
     6121 ggtggagatg atggaggaga cctgaaaaaa ggcatagcgc aggcactgga gaggcccctg
     6181 gtacaggtgc cggatgttga aggcgatgac ggcggtggac aggcccacca ccaccaggaa
     6241 aaagcgcagc tcatcgctct gaagcacttc ccggaagcgg cggcacagca gcaggaagta
     6301 catggcgaaa ttgatggaga acagcagggc gaagacggcg atgatcatct ccagcacggg
     6361 gtcgccatag gcgcctacac tggcattgcg gttggagaag ccgccggtgc ccgccgtgga
     6421 gaaggcgtgg ataaaggagt cgtacagagg catgccggcc agcttcagca gggcgacctc
     6481 ggccagtgtc atggcgcagt agatcccgta gaggatcttg gaggtctggg actgcttggg
     6541 gaccagcttg gaaaagaccg ggcccggggt ttccgcttgg gtgaggtagt gagaacggat
     6601 gcccagggag gggagcagag cggtgagcag caccagcacg cccatgccgc ccatccagtg
     6661 ggtgaatgcc ctccagaaga ggatgccacg gggctgggcc tcgatgtcag tgaggatggt
     6721 ggcaccggtg gtagtgaagc cggacaccga ctcaaacagg cagtccacaa aggaggggaa
     6781 ggtgccggag aagtagaaag ggagcgcccc catcacgccg gtgaccagcc agatcaggcc
     6841 cacggcaaag aagccctccc gggcgtagaa atgccgggtg gcgggtacag cggacagggc
     6901 cagtcccaga atggccatca gcaggatggt caacaggaag ggggcggggt cctcctggta
     6961 cagcagggcg accgccagcg gcaggagcat gaagaatgcc tcgaccagca ggacgcggcc
     7021 caccagccgg gataccaatt tcagattcat gacatgcccc ccggggcaga gaaggtgtgc
     7081 tcgccctcgt catagatgtc gttgagatcc tgaagacgcc tgtctcggga gatgaggatg
     7141 acagagtccc cctcttggat ggacgtggag ccttccggga taatgatctc gcctccccgg
     7201 gccaccacgg ccaccagaat accgggcttc agggacaggt cccgcagggg cgtgcccaga
     7261 tggcgggtgg aggcggtgac ggtaaactcc atggcctcgg cggccccatc cgcgatgcgg
     7321 tacagagcgt tcatcacg
//
LOCUS       DBMDSJ010000033         7286 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3091_length_7286_cov_1.742241, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000033 DBMDSJ010000000
VERSION     DBMDSJ010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_7286_cov_1.742241"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(45..953)
                     /locus_tag="ACZPAQ_01540"
     CDS             complement(45..953)
                     /locus_tag="ACZPAQ_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR0902689.1"
                     /translation="MKKTRVWPALLAALVILAGCAAAPPEESDPPTEEFVFTRENMPR
                     LNGSTSAVPLAEAVCSVLLGESREEVADLVQFSRTTASYEALMAGEADLLLAGEPEQA
                     VMERLEAGDRWLLTPFATDALVFVVNEDNPVDGLTTEQLQKIYTGEITNWSQVGGEDR
                     DIIPFQRNPEAGSQALMKKLVMDGLEMMEPPVDYTAGSMSSLLEGVRQYDNSPAAIGY
                     TVYYYAHDMEMAQGLKLLSIDGTAPSPDTIRSGEYPFLNPYYAVMDKDEAEDSPARIL
                     YDWLLGEEGQSLVAQEGYVSILEQEG"
     gene            complement(1101..1673)
                     /locus_tag="ACZPAQ_01545"
     CDS             complement(1101..1673)
                     /locus_tag="ACZPAQ_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR0902690.1"
                     /translation="MYIDPTVYQGRPSDDRIPQELAVYDKLEELGISFTRVDHDHADT
                     MEDCLLIERTLGARICKNLFLCNRQQTDFYLLMMPGDKPFKTKDLSPLLGCSRLSFAG
                     PEHMERLLRTIPGSVSALELMFDTQAQVRLVIDRDLLKDRYLSGHPGISTSTLCLERE
                     EMIRFVEATGHTPTYLDLPDPRLSEQGGGL"
     gene            complement(1880..3181)
                     /gene="eno"
                     /locus_tag="ACZPAQ_01550"
     CDS             complement(1880..3181)
                     /gene="eno"
                     /locus_tag="ACZPAQ_01550"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR0902691.1"
                     /translation="MKQIIEIVDVLGREILDSRGNPTVEVEVYLEDGTVGRAAVPSGA
                     STGIYEACELRDGDKGRYQGKGVVTAVNNVNTEIAEALIGTNVLDQVAIDKMLIDLDG
                     TPNKSRLGANAILGASLACAKAAAESLGVSLYSYIGGTNAKMLPVPMMNILNGGAHAT
                     NNVEIQEFMIMPVGAPSFHEALRMCTEVFHTLKNTLKENGTPAAGVGDEGGYAPNLKK
                     DEDALKVIVKAIEEAGYKPGEDFKIAIDAASSEWWDEEQKCYIQPKSGKKLTQQQLVN
                     MWKKFADTYPIVSLEDGMAETDWEGWAMLTRAIGDKVQLVGDDLFVTNVERLATGIEK
                     KVGNSILIKVNQIGTLTETLDAIQMANRAGYTAIVSHRSGETEDTTIADISVATNAGQ
                     IKTGAPSRTDRVAKYNQLLRIEEELGDIAEYPGMKAFFNLK"
     assembly_gap    3186..3195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3280..4794)
                     /gene="gpmI"
                     /locus_tag="ACZPAQ_01555"
     CDS             complement(3280..4794)
                     /gene="gpmI"
                     /locus_tag="ACZPAQ_01555"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR0902692.1"
                     /translation="MKTPTTLIIMDGFGLGPKYPGNAVENTPKPHLENIFKECPGCRL
                     SASGLDVGLPEGQMGNSEVGHTNIGAGRVVFQDLPHISRDIDSGEFFKNPAYLEAMEH
                     CREWGTALHLMGLLSDGGVHSHITHLFALVKMAKEQGLEKVYVHCFLDGRDVPPSSGK
                     SYVEQLQAKLDELGTGRIATVMGRYYAMDRDKRWDRVQRAYDAIALGEGIFEEDPAAA
                     VQKSYDSGVTDEFMEPVVCAKGAQVRDNDSIIFYNFRPDRAREITRCFVDEDFQDVER
                     KKGFVPVDFVCTTEYDATMPNVTVAYPRQKLENIFGEYISKLGLTQLRIAETEKYAHV
                     TFFFNGGVETVFPGEDRVLIASPKVATYDLQPEMSAYQVTEEAVKRIESGAYDVIILN
                     FANCDMVGHTGVYEAACRAVTAVDECVGRVVEATSRMGGVSLITADHGNAERMADEDG
                     EPFTAHTTNLVPFYIVGASVRLRDGRLADIAPTMLDLMGLEKPKEMDGETLIVN"
     gene            complement(4998..5771)
                     /gene="tpiA"
                     /locus_tag="ACZPAQ_01560"
     CDS             complement(4998..5771)
                     /gene="tpiA"
                     /locus_tag="ACZPAQ_01560"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR0902693.1"
                     /translation="MNRRYRKTIIAGNWKMNNTLSQTKQFAEEIKPIMPRGKWCNVVL
                     CVPAVNITTAVRLFKDCHIAIGAETCHYEDHGAYTGEITAEMIKEAGAKYVIIGHSER
                     RQYYNETDFTVNKKVHAALEVGLNPIVCVGESLEQRELGVTMELIAYQVKAALAGVPA
                     DKMRHVVIAYEPLWAIGTGKTATAEQAGEVCQAIRAVIRKLYGAHVARSVTIQYGGSM
                     NAKNAAELLAQPDVDGGLIGGASLKPADFVEIINAANQE"
     gene            complement(6086..7282)
                     /locus_tag="ACZPAQ_01565"
     CDS             complement(6086..7282)
                     /locus_tag="ACZPAQ_01565"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR0902694.1"
                     /translation="MNYNKKTVRDIDVAGKKVLLRCDFNVPMAKDGSGVITDDKRIRA
                     ALPTIQYLLEQNAAVVACSHMGKPKGEVKPELSLKPVAARLSELLGKPVIMAEDVVGP
                     DARAKAAALKPGEILLLENTRFEPGETKNDPALAKAMASLADVYVSDAFGAVHRAHAS
                     TAGVADYLPAVSGFLIQKELEVIGGALADPKRPLVAILGGSKVSSKIGVINNLLEIAD
                     TIIIGGGMAYTFSAAQGGKVGDSLLEADWESYANEMVQKAAAKGVKLLLPVDTVCADA
                     FAPDANSQVVKAGEIPDGWQGLDIGPETVKLYCGAVAGAGTVIWNGPMGVFEFPAFAK
                     GTEAVAEALSKTDAITIIGGGDSAAAVQQLGYADKMTHISTGGGASLEFMEGKELPGV
                     ACLLDK"
BASE COUNT         1217 a         2330 c         2259 g         1470 t
ORIGIN      
        1 cagcaccagg gccaggaccc aggccgcgcc gcgccctctc atattcaccc ctcctgttcc
       61 agaatggaca catagccctc ctgggccacc agggactggc cctcctctcc cagcagccag
      121 tcgtagagga tgcgggcggg gctgtcctcc gcctcgtcct tgtccatgac agcgtagtaa
      181 ggattgagga aaggatattc cccgctgcgg atggtgtccg ggctgggggc ggtcccgtcg
      241 atggagagca gcttcagtcc ctgggccatc tccatatcgt gggcatagta gtagacggtg
      301 tagccgatgg cggcggggct gttgtcatac tgccgcaccc cctccagcag agaggacatg
      361 gagcccgctg tgtagtccac cggaggctcc atcatctcca ggccgtccat caccagcttc
      421 ttcatcaggg cctggctccc tgcctccggg ttgcgctgga aggggatgat gtcccggtcc
      481 tcgcccccca cctggctcca gttggtgatc tccccggtat agatcttttg gagctgctcc
      541 gtggtcaggc cgtccaccgg gttgtcctcg ttgactacaa agaccagtgc gtcggtggcg
      601 aagggggtca ggagccaccg gtcccccgcc tccagccgct ccatcaccgc ctgctctggc
      661 tcgccggcca gcagcaggtc ggcctccccc gccataagtg cctcatagga ggcggtggtg
      721 cgggagaact ggaccagatc ggccacctct tcccggctct ctcccagcag gacggagcac
      781 accgcctccg ccagggggac ggcggaggtg gacccgttga gccggggcat attctcccgg
      841 gtaaagacga actcctctgt cggcggatcg ctctcctccg gcggagcggc ggcgcatccc
      901 gccaggatca ccagggccgc cagcagggcg ggccatacgc gggtcttctt catcctgatc
      961 ctcctctctt ccagcatggc tcggggcaaa aggaaagccc ctttttcctt ctgcctccat
     1021 cataccagag ccaggggaaa aaggggttgc gaaaggtttg caaaccggtg acaaagagag
     1081 acaaaacggc tacgatttcg ttacagacct ccgccctgct ccgacaggcg cgggtccggc
     1141 aggtccagat aggtgggggt atggccggtg gcctccacga agcggatcat ctcctcccgc
     1201 tccaggcaca gggtggaggt ggagatcccc gggtgtccgc tgagataccg gtccttcagc
     1261 aggtcccggt cgatgaccag ccgtacctgt gcctgcgtat cgaacatcag ctccagggcg
     1321 ctcaccgagc cggggatggt gcgcagcagg cgctccatat gctccgggcc ggcaaaagag
     1381 agtcgggagc agcccagcag cggggagagg tccttggtct tgaagggctt gtcccccggc
     1441 atcatcagca ggtaaaagtc agtctgctgc cggttgcaca gaaacaggtt cttgcagatg
     1501 cgggccccca gggtccgctc gatgagcagg cagtcctcca tggtgtcggc atggtcgtga
     1561 tccacccggg tgaaggagat gcccagctct tccagtttgt cgtagacagc cagctcctgg
     1621 gggatacggt catcggaggg gcgtccctgg tagacggtgg ggtcgatgta catagcggtc
     1681 cttccttttc ttcaatggtc ttgctgcatc ataccacagc tggaacaaaa agtacagtcc
     1741 ggcggcacag aagggactgg atggccgtcg ccgcttccca gtgcacggcg cgccccatct
     1801 gcgatggggc ccccaggagc tggacgacag gaagagcaaa gccgcccgat tgggcggctt
     1861 tgcagcaaaa tttgaaaact tacttcaggt tgaagaacgc cttcatgccg gggtactccg
     1921 cgatgtcgcc cagctcctcc tcgatgcgga gcagctggtt gtacttggcc acgcggtcgg
     1981 tgcggctggg ggccccggtc ttgatctgac cggcgttggt agccacggag atgtcggcga
     2041 tggtggtatc ctcggtctcc ccggagcggt gggacacgat ggcggtgtag ccggcccggt
     2101 ttgccatctg gatggcatcc agggtctcgg tcagggtgcc gatctggttg accttgatga
     2161 ggatggagtt gcccaccttc ttctcgatgc cggtggccag gcgctccacg ttggtgacga
     2221 acaggtcgtc gcccaccagc tggaccttgt cgccgatggc acgggtcagc atagcccagc
     2281 cctcccagtc ggtctcggcc atgccgtcct ccagggagac gatggggtag gtgtcggcaa
     2341 acttcttcca catgttcacc agctgctgct gggtcagctt cttgccggac ttgggctgga
     2401 tatagcactt ctgctcctcg tcccaccact cggaggaggc ggcgtcgatg gcgatcttga
     2461 agtcctcacc gggcttgtag ccggcctcct cgatggcctt cacgatgacc ttcagggcat
     2521 cctcgtcctt cttcaggttg ggggcgtagc cgccctcgtc gcccacaccg gcggcggggg
     2581 tgccgttctc cttcagggtg ttcttcaaag tgtggaacac ctcagtgcac atgcgcagag
     2641 cctcatggaa actgggggcc cccacgggca tgatcatgaa ttcctggatc tccacgttgt
     2701 tggtggcatg ggcaccgccg tttaggatgt tcatcatggg cacaggcagc atcttagcgt
     2761 tggtcccgcc gatgtagctg tacagggaga cacccaggct ctctgccgcg gccttggcgc
     2821 aggccaggga ggcgccaagg atagcgttgg cgcccaggcg gctcttgttg ggagtgccat
     2881 ccaggtcgat gagcatcttg tcgatggcca cctggtccag cacgttggtg ccgatgaggg
     2941 cctcagcgat ctcggtattc acgttgttca ccgcggtcac cacgcccttg ccctggtaac
     3001 ggcccttgtc gccgtcccgc agctcgcagg cctcatagat gccggtggag gcgccggaag
     3061 ggaccgcggc gcggcccaca gtgccgtcct ccaggtagac ctccacctcc acggtggggt
     3121 tgccccggct gtccaggatc tcgcggccca aaacgtctac gatctcaatg atctgcttca
     3181 tgatannnnn nnnnnatggg cgggtcctcc cgcccggagc gtatatgccc tgtgcagcag
     3241 ctccgcaccg gtgcatcggt ccagcggggg cccagtccgt cagttgacga tcagagtctc
     3301 cccgtccatc tccttgggct tttccaggcc catcaggtcc agcatggtgg gggcgatatc
     3361 ggccaggcgg ccgtcccgca ggcggacgct ggcccccacg atatagaagg gcaccaggtt
     3421 ggtggtgtgg gcggtgaagg gctccccatc ctcgtccgcc atccgctcgg cattgccgtg
     3481 gtcggcggta atgagagaga cgccgcccat gcgggaggtg gcttccacca cccggcccac
     3541 gcactcgtcc acggcagtca ccgccctgca ggcagcctca tacacgccgg tgtggcccac
     3601 catgtcgcag ttggcaaagt tcaggatgat gacatcatag gctccgctct cgatgcgctt
     3661 gaccgcctcc tccgtcacct gataggcgct catctccggc tggaggtcgt aggtagccac
     3721 cttgggggaa gcgatgagca cccggtcctc cccgggaaag accgtctcaa cgccgccgtt
     3781 gaagaagaag gtgacgtggg catacttctc cgtctcagcg atgcgcagct gggtcagccc
     3841 cagcttgctg atatactcgc cgaagatgtt ctccagcttc tgccgggggt aggccaccgt
     3901 cacattgggc atggtagcgt cgtactcggt ggtacagaca aaatccaccg ggacgaagcc
     3961 ctttttccgc tccacatcct gaaagtcctc atccacaaag cagcgggtga tctcccgggc
     4021 ccggtcgggg cggaagttgt agaagatgat ggagtcgttg tcccggacct gagccccttt
     4081 ggcgcagacc accggctcca tgaactcgtc ggtgacgccg gagtcatagg acttctggac
     4141 agcggcggcg gggtcctcct caaagatccc ctcccccagg gcgatggcat cataggcccg
     4201 ctgcacccgg tcccaccgct tgtcccggtc catggcgtag tagcggccca tgacggtggc
     4261 gatcctgccg gtgcccagct catccagctt ggcctggagc tgctccacat agctctttcc
     4321 agagctgggg ggcacgtcgc ggccatccag gaagcagtgg acatacacct tctccagccc
     4381 ctgctccttg gccatcttta ccagggcgaa caggtgggta atgtggctgt ggacgccgcc
     4441 gtcggacagc aggcccatca ggtgcagggc ggtcccccat tcccggcagt gctccatggc
     4501 ctccaaatag gcaggattct tgaagaactc cccgctgtcg atgtcccggc tgatgtgggg
     4561 caggtcctgg aataccaccc ggcccgcgcc gatgttggtg tggcccacct cgctgttgcc
     4621 catctgcccc tcaggcaggc ccacgtccag gccggaggca gacaggcggc acccgggaca
     4681 ctccttgaag atattctcca gatggggctt cggcgtattt tccaccgcat tgcccggata
     4741 tttggggccc aggccaaagc cgtccatgat gatcaaagtt gttggtgttt tcatagaaac
     4801 cctttcttga gagaatccag agggaagggg catgaagagc aggctcccgc tccgcattcc
     4861 gtgtcttcct ctccgtgtgt ttcgatggtg cgggatgggg agaggcaaaa tgcctgccgc
     4921 tcccccgctc cgcctctccc aacaaaaccc gctgcgctgg gctttcgttg gggacccatc
     4981 cggcatggac ggctcgctta ctcctggttt gccgcgttga tgatctccac gaagtccgca
     5041 ggcttcaggg aggctccgcc aatcaggccg ccatccacgt cgggctgggc cagcagctcg
     5101 gcggcgttct tggcgttcat ggagccgccg tactggatgg tgacggagcg ggccacatgg
     5161 gcgccgtaca gcttgcggat gacagcacgg atagcctggc acacctcacc agcctgctcg
     5221 gcggtggcgg tcttgccggt gccgatggcc cacagaggct cataggcgat gaccacatgg
     5281 cgcatcttgt cggcgggcac gccggccaag gcagccttca cctgataggc gatcagctcc
     5341 atggtcactc ccagctcccg ctgctccagg ctctcgccca cacagacgat ggggttcagc
     5401 cccacctcca gggcggcgtg gaccttctta ttgacggtga agtcggtctc gttgtagtac
     5461 tggcggcgct cagagtggcc aatgataaca tacttggccc ccgcctcctt gatcatctcc
     5521 gcagtgatct caccggtata ggcaccgtgg tcctcatagt ggcaggtctc cgcgccaatg
     5581 gcgatgtggc agtccttgaa caggcggacg gcggtggtga tattgacggc cgggacgcac
     5641 aggaccacgt tgcaccactt tccccggggc ataatgggct tgatctcctc ggcgaactgt
     5701 ttggtctggg agagggtgtt gttcatcttc cagtttccgg caataatggt cttgcgatat
     5761 cttctgttca tggcggtaaa gcccctttct atttttcctt ctcttcctag tttgttacac
     5821 acttgagcct agtctgcccg gatcttggct tcccagctgt gatttgtccc gcactacgcc
     5881 gcaaaactta aaagcatcac tacaagaagg acccccagaa tggtcagaag gatatttctc
     5941 atttttttgt tcatgcacga tccctctttc ctgtatctca gcgtcgtcaa agcatgcgcc
     6001 atatttttca ctttcgcctg ccggtgaaat cccatcccca tttcgctggg ctcgcgcggg
     6061 tccccggagc tgcccgggca gttctttatt tatccagcag gcaggctacg ccgggcagct
     6121 ctttgccctc catgaattcc aggctggcgc cgccgccggt gctgatgtgg gtcatcttgt
     6181 cggcatagcc cagctgctgc actgcggccg cggagtcgcc gccgccgatg atggtgatgg
     6241 cgtccgtctt tgacagagcc tcggccaccg cctcagtccc cttggcaaag gcggggaact
     6301 cgaacacacc catagggccg ttccagatca ctgtgcccgc gccggccacg gcgccgcagt
     6361 acagcttcac ggtctcgggt ccgatgtcca ggccctgcca gccgtcgggg atctccccgg
     6421 ccttgaccac ctggctgttg gcgtcgggtg caaaggcgtc ggcgcacacg gtgtccactg
     6481 gcagaagcag cttcacgccc ttagccgccg ctttctggac catctcattg gcgtagctct
     6541 cccaatcggc ctccagcagg gagtccccca ccttgccgcc ctgggcggcg gagaaggtgt
     6601 aggccatgcc gccgccgatg atgatggtgt cggcgatctc cagcaggttg ttgatgaccc
     6661 cgatcttgct ggataccttg gagccgccca ggatggccac cagggggcgc ttgggatcgg
     6721 ccagggcgcc gccgataact tccagctcct tctggatgag aaagccggac acagcgggca
     6781 ggtagtcggc cacgccggcg gtggaggcat gggcgcggtg gaccgcgccg aaggcgtcgg
     6841 acacgtacac gtcggccaga gaggccatgg ccttggccag ggcggggtcg ttcttggtct
     6901 cgccgggctc aaatctggta ttttccagca gcaggatctc gccgggcttc agggcggcgg
     6961 ccttggcccg ggcatcgggg cccaccacgt cctccgccat gatgaccggc ttgcccagca
     7021 gctcgctcag gcgggcggcc accggcttca gagacagctc gggcttgacc tcgcccttgg
     7081 gcttgcccat gtgggagcag gcgaccaccg cagcgttctg ctccagcaga tactggatgg
     7141 tgggcagggc ggcacggatg cgcttgtcgt cggtgatgac gccgctgccg tccttggcca
     7201 tgggcacgtt gaagtcgcac cgcagcagca cctttttgcc cgccacatcg atgtcgcgca
     7261 ctgtcttctt gttgtagttc attctt
//
LOCUS       DBMDSJ010000034         7191 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3134_length_7191_cov_2.013258, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000034 DBMDSJ010000000
VERSION     DBMDSJ010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3134_length_7191_cov_2.013258"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            135..722
                     /locus_tag="ACZPAQ_01570"
     CDS             135..722
                     /locus_tag="ACZPAQ_01570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0902695.1"
                     /translation="MNTPELETERLRLRRFTAEDAAAILAIFGDVEANTFLPWFPLKS
                     LEEARQLFERQYQPVYRAERGYQYAICLKEDDIPIGYVKVDLDESHDLGYGLRHEFWH
                     RGIAAEAARALVEQVRRDGLPYLTATHDVNNPNSGAVMKKLGMVYQYSYEEQWHPKDI
                     PVLFRLYQMDLDGNSGRPHLRYWERSRVHMVEQGI"
     gene            complement(1283..2158)
                     /locus_tag="ACZPAQ_01575"
     CDS             complement(1283..2158)
                     /locus_tag="ACZPAQ_01575"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR0902696.1"
                     /translation="MSRQIAVIGLGLIGGSIAMGLKEFEDFKRVGVDVSQPTLRYALE
                     QGIVDQVCEDPREALAQADLVFLCMHPRGIQEFLSEHRDHFRPGSLVTDVCGVKGAIV
                     DAARTLPSSVSFLGGHPMAGTEFSGIQHALPNMFQGAHYILTPDEGTQPEHLALMERV
                     ARHLGCADVVRTTPEQHDAIIAYTSQMMHIIAVSVCDDPSLFAYQGFEGDSFRGCTRV
                     AALDVPLWTQLFSLNAPALTQALETLEDNLRLYRQVIQSGDTRQLADKLAFSAARKRK
                     MNQLEAERGATGRGA"
     gene            complement(2260..2952)
                     /locus_tag="ACZPAQ_01580"
     CDS             complement(2260..2952)
                     /locus_tag="ACZPAQ_01580"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR0902697.1"
                     /translation="MPALLLAALCRPGPGPAAEAGGGVSVTVPEEPPLVALTFDDGPR
                     TSTTSRLLDGLALREIQATFFLVGDRIQGQEALVRRMAEDGHQIGVHSYDHSCISDLS
                     RGDFDLQVGRTRGVLSAVLGSGDFWLRPPYGIIDDAGLRWAGSPVVLWSVDPEDWKDQ
                     DTGRVVGSVLSHVEDGSIILLHDIYDSSVDAALQIADALSGQGYCFVTVEQLLQAKGI
                     QPQDGTVYRCAK"
     gene            complement(3130..4302)
                     /locus_tag="ACZPAQ_01585"
     CDS             complement(3130..4302)
                     /locus_tag="ACZPAQ_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR0902698.1"
                     /translation="MYRIKTMNKIASAGLDQLDKSRFQVGEDVENEDGILVRSAKMHD
                     YVFPDALRAIARAGAGTNNIPIDRCSESGIVVFNTPGANANAVKELVIAALLIASRDI
                     VGGVDWVQEQAHTPNVDVAAAVEKGKSAFVGPELYRKTLGVIGLGAIGALVCNIGLDL
                     GMDVYGYDPYLSVDAALRLDRHVHVVKDVNELYKVSDYVTIHVPYTSSTKDFINGDAI
                     AKMKGGVRILNLARGELVCDQDMIAALETGRVAKYVTDFPNDTIATVKNVIAMPHLGA
                     STPESEENCAVMAARQLQDYLVNGNIRNSVNLPNLEQEWSGISRLCLIHKNVPAMLTN
                     IMSTLSNDGINVENMTNKSKKDYAYTIVDVNTRITDQVANELRAIPGVIRVRVLNH"
     gene            complement(4346..4525)
                     /locus_tag="ACZPAQ_01590"
     CDS             complement(4346..4525)
                     /locus_tag="ACZPAQ_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009983359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HIR0902699.1"
                     /translation="MAAKENNRFVKIYAQGALVQTEIWVDTVTGVQYLFHRDGNASGL
                     TPLLGPDGKPVIAPQ"
     gene            complement(4577..5662)
                     /gene="serC"
                     /locus_tag="ACZPAQ_01595"
     CDS             complement(4577..5662)
                     /gene="serC"
                     /locus_tag="ACZPAQ_01595"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR0902700.1"
                     /translation="MSDQRVYNFSAGPSMLPLSVLERAGAEITNYHGSGMSVMEMSHR
                     SKVFIQIFEETQAKLRRLMKVPDNYKILFLQTGATGQFSMIPLNLIGKTGRADYAVTG
                     NFSNLAYKEAQKYGQISLAASSEDRNHTYIPSQDQLKLDPEASYFYYCANNTVYGTEW
                     QYVPETGSVPLVCDMSSDILSRPVDVSKFGIIYAGAQKNMAPAGLTVVIIREDLAGHE
                     LPYTPVMMSYKAMIDKDSMYNTPPCWCIYMLGLTLDWLDEQGGIEGMEQIKHGKAQML
                     YDCIDSSKLFTCAAEKPFRSDMNVTFRSASEELDAKFVKEAAEAGFVNLKGHRKVGGM
                     RASIYNAVPVEGVEKLCDFIRAFDKAN"
     gene            complement(5993..6247)
                     /locus_tag="ACZPAQ_01600"
     CDS             complement(5993..6247)
                     /locus_tag="ACZPAQ_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HIR0902701.1"
                     /translation="MTGSLAGAWGVAVMVALAALLLLHRPLGRLLRLAVRSSVGLAAL
                     ALLNQIGLGLGVNLVNALILGVLGVPGLGLLLMLQWVLRT"
     gene            complement(6244..6483)
                     /locus_tag="ACZPAQ_01605"
     CDS             complement(6244..6483)
                     /locus_tag="ACZPAQ_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HIR0902702.1"
                     /translation="MAEAARMFSLRRLERADNVERQELLTSLAHTRALIQQAYRGFNS
                     AEDGDLIESYVFEINALQSRYNYLLRRVKELEAQP"
BASE COUNT         1466 a         2077 c         2282 g         1366 t
ORIGIN      
        1 cggaatggca gggggggcgc attccccggc ggcgaggtgg gcgggccatc ttgcaggggc
       61 cgcctccgat atggtagaat gacctgaccg gaacaaaatg agccaacaga aaaacagaag
      121 gggagcggag cgcaatgaat acaccggagc tggagacgga gcggctgcgc ctgcggcggt
      181 tcacagcgga ggacgcagcg gccatactgg ccatttttgg agacgtggag gccaatacct
      241 tcctcccgtg gttccccctg aagtcgctgg aggaggcgag gcagctcttt gaaaggcagt
      301 accagccggt ctaccgggca gagcggggat accaatacgc gatctgcctc aaggaggacg
      361 acatccccat cggctacgtc aaggtggacc tggacgagag ccacgacctg ggctatgggc
      421 tgcgccatga gttttggcat cggggcatcg ctgcggaggc agcccgggcc ctggtagagc
      481 aggtgaggcg ggacgggctg ccttatctca cagccactca cgatgtgaac aaccccaaca
      541 gcggagcggt gatgaaaaag ttgggaatgg tctatcaata ctcctatgag gagcagtggc
      601 atcccaagga catcccggtg ctgttccggc tctaccagat ggatctggac gggaatagcg
      661 gcaggccaca cctcagatac tgggagcgat cccgggtcca tatggtggag cagggcatat
      721 agccgggcag ggattctctg tttacagaaa gttcacaggg atgacatgat ttcgccttaa
      781 tttctgggta tgataacacc gtacccagaa gaaaactccc atttctccct ctccttttac
      841 aacacacaca ggtctgacac cccggtccca tggaccgggg tgtcagactg ttccggtaca
      901 ggatggggct atttacagaa aattcacatt gcggacatga tttagcagta cttctccgtt
      961 aagataagac cgtacccagt tttcatagga tacctcccat ttctccctct ctttacaaca
     1021 cacacaggtg cagcgccccg gcctcaaggc tggggcgctg tgctgtccgg agagacgcgt
     1081 gctgtttaca gaaagttcat aggcaggtca tgatttcgac gtatttcttc ggtaggatat
     1141 cactgtaccc aagatcacaa agaattctcc catttctccc tctcctttac aacacacaca
     1201 agcagagagc cccggcctaa cggccggggc tctctgctgt catttgtggg gaaagagatt
     1261 ccccaggctc tctgcctgct ggtcatgcgc cccggccggt ggccccccgt tctgcctcca
     1321 gctggttcat cttccgcttc cgggcggcgg agaaggccag cttgtccgcc agctgacggg
     1381 tgtccccgga ctggatgacc tggcggtaga ggcgaaggtt gtcctccaac gtctccagcg
     1441 cctgggtcag ggcgggagcg ttcagggaga agagctgggt ccacaggggg acgtccaggg
     1501 cggccacccg ggtgcagccc cggaaggagt ccccctcgaa gccctggtag gcgaacaggc
     1561 tggggtcgtc gcacaccgag acggcgatga tgtgcatcat ctggctggta taggcgatga
     1621 tggcatcgtg ctgctccggg gtggtgcgca ccacatcggc acagcccagg tgcctggcca
     1681 cccgctccat cagcgcaaga tgctccggct gggtcccctc gtccggggtg aggatgtagt
     1741 gggccccctg gaacatgttg ggcagggcgt gctgaatgcc ggaaaattca gtgcctgcca
     1801 tggggtgccc tcctagaaag gatacactgg agggcagggt ccgggcggca tccacgatgg
     1861 cgcccttcac gccgcataca tcagtgacca ggctgccggg gcggaagtgg tcccgatgct
     1921 cagagaggaa ctcctggatg ccccggggat gcatgcagag gaacaccaga tccgcctggg
     1981 ccagggcctc ccgggggtcc tcgcagacct gatccacaat tccctgctcc agggcgtagc
     2041 gaagggtggg ctggctcaca tccaccccca cccgcttaaa atcttcaaac tctttcagcc
     2101 ccatagcgat ggagccgccg atcaggccca ggccgatgac cgcgatctgt ctgctcataa
     2161 acgatctcct cgattccaaa gtgcgtctgt gggaacgata gcactgtacc acggtttggg
     2221 gcgcaaatca acactttaac acaaaaaagc gttaaatttt tattttgcgc accggtaaac
     2281 agtcccgtcc tggggctgga ttcccttggc ctggaggagc tgctccaccg tgacaaagca
     2341 gtagccctgc ccggagaggg catccgcgat ctggagagcg gcgtccactg agctgtcgta
     2401 gatgtcgtgg agcaggatga tgctgccgtc ctccacatgg ctgaggacgg agcccaccac
     2461 ccgcccagtg tcctggtcct tccagtcctc cgggtctacg gaccacagga caacggggga
     2521 gccggcccac cggagccccg catcgtcaat gattccgtag ggcgggcgga gccagaagtc
     2581 accggagccc agcacggcgg agaggacgcc ccgggttctg cccacctgga ggtcgaagtc
     2641 cccccgagag aggtcagaga tgcagctgtg atcgtaggag tgaacgccga tctggtggcc
     2701 gtcttccgcc atgcgccgga caagggcctc ctgaccttgg atgcggtcgc ccaccaggaa
     2761 gaaggtggcc tggatctccc ggagagccag accgtccagc aggcgggagg tggtggaggt
     2821 tctggggccg tcgtcaaagg tgagagccac cagggggggc tcctccggca ccgtcaccga
     2881 cacgccgccc cctgcctctg cggctggccc tgggccgggc cggcacaggg ccgccagaag
     2941 cagtgcaggc acaaggaccc gccagtattt aaaaaatttt ttcctgcacc acatgatgac
     3001 cgccgccttt cccatcgcgc tcactgttca gtatgtgcgc tttttccggc agcttgctca
     3061 gaaatttctg acagaaaaaa tgccctccgg ccggggccgg agggcaggga aagcggatgg
     3121 ggcggcagat cagtggttca gcacccgcac ccggatcacg ccggggatgg cgcgcagctc
     3181 gttggctacc tggtcggtga tgcgggtgtt cacgtccacg atggtgtagg cgtagtcctt
     3241 tttggatttg ttggtcatgt tctccacgtt gatcccgtcg ttggacaggg tggacatgat
     3301 gttggtgagc atggcgggca cgttcttgtg gatgaggcag aggcgggaga tgccgctcca
     3361 ctcctgctcc agattgggca ggttcaccga gttgcggatg ttgccgttga ccaggtaatc
     3421 ctggagctga cgggcggcca tgacggcgca gttctcctcg ctctccgggg tggaggcgcc
     3481 cagatggggc atggcgatga cgttcttcac cgtggcgatg gtgtcgttgg ggaaatcggt
     3541 gacatacttg gccacccggc cggtctccag ggcggcgatc atgtcctggt cgcacaccag
     3601 ctcgccccgg gccagattca gaatgcggac accgcctttc atcttggcga tggcgtcccc
     3661 gttgatgaag tccttggtgg aactggtgta ggggacgtgg atggtgacat agtcagacac
     3721 cttgtacagc tcgttcacgt ccttgaccac atggacatgg cggtccagcc gcagggcggc
     3781 gtccacagac aggtaggggt cgtagccgta cacgtccatc cccaggtcca ggccgatgtt
     3841 gcacaccagg gcgccgatgg cacccaggcc gatgacaccc agggtcttac ggtagagctc
     3901 agggcccaca aaggcggatt tgcctttctc cacggcggcg gctacatcca catttggagt
     3961 atgagcctgt tcctggaccc agtccacgcc cccgacgatg tcccgggagg cgatgagcag
     4021 ggcggcgatg accagctcct ttactgcgtt ggcgttggca ccgggggtat tgaagaccac
     4081 gatgccggac tcggagcagc ggtcgatggg aatgttgttg gtgccggcgc ccgcccgggc
     4141 gatggcacgc agggcatccg ggaagacgta gtcgtgcatc ttggcgctgc gcaccaggat
     4201 gccgtcctcg ttctccacat cctcccccac ctggaagcgg ctcttgtcca gctgatccag
     4261 gccggcggac gcaatcttgt tcatggtttt gatgcggtac atatggcaat tacctcgaat
     4321 cctgatttct gatggaaggg gcggatcact ggggagcgat cacaggcttc ccgtccggcc
     4381 ccagcagcgg ggtgagccca gaggcatttc cgtcccggtg aaagagatac tgcaccccgg
     4441 tgacagtatc cacccagatc tcggtctgga ccagggcacc ctgggcgtag atcttgacga
     4501 agcggttgtt ttcttttgcg gccatggaag cacccctgtt tggtctgcaa gcggaagatc
     4561 gaatgggaac agggcctcag ttggccttat cgaaggcccg gatgaagtca cagagcttct
     4621 ccacgccttc cacagggacg gcgttgtaga tggaggcccg catgccgccc actttgcggt
     4681 gccccttcag attgacgaag ccggcttcgg ccgcctcctt gacgaacttg gcgtccagct
     4741 cctcactggc ggagcggaaa gtgacgttca tatcggagcg gaagggcttc tcggcggcgc
     4801 aggtgaacag cttggagctg tcgatgcagt cgtagagcat ctgggcctta ccgtgcttga
     4861 tctgctccat gccctcgatg cccccctgct cgtccagcca gtccagggtc aggcccagca
     4921 tgtagatgca ccagcagggc ggggtgttgt acatggagtc cttgtcgatc atggccttgt
     4981 agctcatcat cacgggggtg tagggcagct cgtggccggc cagatcctcc cggatgatga
     5041 ccacggtcag gccggcgggg gccatgttct tctgggcgcc ggcgtagatg atgccgaact
     5101 tggagacgtc cacggggcgg gagaggatgt cagaggacat gtcgcacacc agggggacgc
     5161 tgccggtttc ggggacatac tgccactcgg taccgtagac ggtgttgttg gcgcagtaat
     5221 agaagtagct ggcctccggg tccagcttca gctggtcctg gctggggata taggtgtggt
     5281 tgcggtcctc ggaggaggcg gccaggctga tctggccgta cttctgggcc tccttgtagg
     5341 ccaggttgga gaagttgccg gtgacggcgt agtctgccct gcccgtcttg ccgatcaggt
     5401 tcagggggat catggagaac tggccggtgg cgccggtctg gagaaacagg atcttatagt
     5461 tgtccggcac cttcatcagg cggcggagct tggcctgggt ctcctcaaag atctgaatga
     5521 acacctttga gcggtgactc atctccatca cagacattcc tgatccatgg tagttggtga
     5581 tctcggcccc ggcacgctca aggacggaca ggggaagcat ggaaggtcct gcggagaagt
     5641 tgtaaacacg ctggtcgctc attttgggag agcccctttc aaattaagat cttgccagct
     5701 ctgctcccgc acaggggaag ggaaacaaaa atgacaatgt atccctatta tagtgaagga
     5761 aaatttctct gtcaatgccc ccgtccgtcc ccaaagaggc cttttgggat aaaaaagccc
     5821 gcgttttttg gaaaataatt ttcaaatgca ggatatgggg aaaaaagccg caaaatccgg
     5881 ggagagaacg ggaaaaaagt gggaaaaatg ccgcccctgc atgaaaggct gcagaggcgg
     5941 catgaagaaa tgagatgggt ccatgggatt atttcacata gggaccgggc cctcaggtcc
     6001 gcaggaccca ctggagcatc aggagcaggc ccaggccagg gacacccagc acacccagga
     6061 tcagggcgtt gaccaggttc acccccagcc ccagtccgat ctggttcagc agggccagcg
     6121 ctgccagccc cacagaggag cgcaccgcca gccggagcag cctgcccagc gggcggtgga
     6181 gcaggagcag ggcggccagc gccaccataa ctgccacgcc ccaggccccg gccagggagc
     6241 cggtcatggc tgggcctcca gctccttgac ccggcgcagc agatagttgt accgggactg
     6301 gagggcattg atctcaaaaa cgtaggattc aatgagatct ccgtcctccg cactgttgaa
     6361 gccccggtag gcctgctgga tcagggcccg ggtgtgggcc agagaggtca ggagctcctg
     6421 ccgctccaca ttgtcagcgc gctccagacg gcgcagggaa aacatgcggg ccgcttccgc
     6481 catgagattg tcctcctttc ggccccggca caccggggca cggctggtct tgtcccttgt
     6541 caatgaaagg tatgatatag tctggtcaaa tattccattg attaaacaca cataggagga
     6601 aaaggagggg agatactaac cccggaccgt gtatcaaact tcctgacctg tgctccagag
     6661 ggcccgatgg cgaaaggcga aaaggagaac ggagaacgct ggggccgtcc catccgggag
     6721 ggctgaacag ccgccggact tttccggccc atccttttta agccggtgct ggaccgcttt
     6781 tgagccgcag accggggagg gcgtggggga ccccgcagaa ggcgggccga cccgcaatgc
     6841 cgaagcaccg tccacacaga cccagtccct ccccgtcccg gacggaggag ctgacagagt
     6901 caaaacgggg ccgccgggac accggcggcc ccctgagact gtcggaaaag tggctttgcc
     6961 gcttttggaa gaaagatgca gtctgcaagt gttttgctcg gcgcacatgt cgccggggaa
     7021 aacagatcag aacttgatcc gcacctgcgg gcgcaagctc tgcgaggctt tttccacaga
     7081 cagagggggc cgccgatatc ccggcagtcc cctctgctgt ttcacaatag taaaccgaga
     7141 aaaaataaag ttgacaatcc ggggaagcgg tagtaaacta ttgcagaaaa a
//
LOCUS       DBMDSJ010000035         7043 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3195_length_7043_cov_1.589023, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000035 DBMDSJ010000000
VERSION     DBMDSJ010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3195_length_7043_cov_1.589023"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(130..1455)
                     /locus_tag="ACZPAQ_01610"
     CDS             complement(130..1455)
                     /locus_tag="ACZPAQ_01610"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR0902703.1"
                     /translation="MIGRFFVVGMFMMINLGLAVGMMFAALFLLRPLLVRILSPGQRV
                     ALWVVGWISLYSPSFYGLLSLVHILPVTFRDLITGRVKDGGWTTSIPAYLPMDYHGAG
                     VYHLSLPGGAVVPVEIGEGIAGAAALLCLGVLVLGIVFSCRQDRRLKELLRRGELLGG
                     EEALTKHVLQEETRKIQIRLVEGLPTSFLTSRGWLGSVEYVICLQRELSPEQRALVLR
                     HELEHIRLGHLWFKGWANMGVFLYWWDPLIWLGYKYFCRDLELACDAAVLKELEPEER
                     REYARTLLEMGAGRSLWSTPLCFGECDTAVRVKTAARPQRTGGAWRGLSWLTTVLLFL
                     FFLGGPTQGGLAEDALLTWERGGGTVDTLVAHTMDQVRQDLGLGPILALQVWCVAEDG
                     EHVWVVMELLEQGWVGAEYFWYPARDRLYFTHAEVLKKPPDLTGAVPLS"
     gene            complement(1482..2801)
                     /locus_tag="ACZPAQ_01615"
     CDS             complement(1482..2801)
                     /locus_tag="ACZPAQ_01615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR0902704.1"
                     /translation="MDGLNTWLTIFLNHLNAGLALGVVLAVLLLLRPLLLRLLTPQQR
                     VAVWYVAWSIGIFGNLWGLTSLVRVLPVTVWDLLGPRPGGIFGAPAFLPPAYDGPGEY
                     PVVLPGGETAAVELNSGPLLALSLLWAAVAVGLLLWYCHRSFALMCWGRQGSELEEKE
                     LVALGADRLELWMQPGKIRAWITPGLPTSFVMGDSVYLQAELPKEQLSLVLRHELNHI
                     YLRHGYFSGLRGACLLLHWWNPILWLAFRYMGRDMELACDQRTLDQLEPEQRKEYAKT
                     LVELGAGRRLWEAPLCFGECDGALRVRAAVAWRPLTPVRRLTGLFCAALVFLLFAGAP
                     AVPGGVTETDLSRNLTQMEEAPEKVVEELIRRLEEKGDLLSGVSIAAGWWKWEGTGGR
                     IYLETSVGTWRAFRCVLGESGKMGMIQLRQEGRPHDLADCTQFYPVD"
     gene            complement(2804..3157)
                     /locus_tag="ACZPAQ_01620"
     CDS             complement(2804..3157)
                     /locus_tag="ACZPAQ_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR0902705.1"
                     /translation="MEKLFDGEYRLMEVLWESAPVNSTQLVTLCREQLGWTKSTTYTV
                     LRKLCRKGAARNERAVVTPLLTREQVIRAQGEELAVKAGGMAPFLNAFFSGRKLTMEE
                     ALELKDLIDQHTWED"
     gene            complement(3440..5650)
                     /gene="csaB"
                     /locus_tag="ACZPAQ_01625"
     CDS             complement(3440..5650)
                     /gene="csaB"
                     /locus_tag="ACZPAQ_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HIR0902706.1"
                     /translation="MKILMATMGLDIGGAETHIVELDKELKAEGHDIVVASNGGVYVP
                     EIVAAGIRHYQVPMHRRDAGCMLRSRKLLKEIIRTERPDVVHAHARIPAFLCGTLQRT
                     MKFPFVTTAHWVFTTKGGLRYLTNWGQRTVAVSDDIKAYLIREYGVPEEHISVTINGI
                     DTEKFSPQTSGQSVVEEFGLDRSRPVVSYVSRMDEDRALVARQLIQIAEELDRAVPGV
                     QLLIAGGGNVFRELKEQAEAVNARLGRTCVVMTGPRTDINQIVAAGDIFVGVSRSALE
                     AMSAAKPVIVAGNEGYQGLFGPEKLEEARLGNFCCRGLPMSTPERLLADMAAVLSLSP
                     EQKAAAGAYGREVIFQYYSVRRMAADCLAMYEQVRRRKYQVVMSGYYGFSNAGDDAIL
                     EAIHQSILEASGEVGVTVLSNDPRETQRHYGMNAIPRFRFWKVFGALRRCDALLSGGG
                     SLLQDRTSTRSLLYYLSIVRCAEWMGKPVMLYANGIGPVQKPANRRRVKRAVERAALV
                     TLRDHSSARELVEMGVERPDLHVTADPVFNLDPAPKERAGELLRSAGLERGTPFAAVS
                     VRDWPDTGSFAGELAALCDHLYRTCGMEILFLMMQPCRDRAATAQVRSAMECPSHLLD
                     ATCTPRELMAVLGEGRLCLAMRLHTLIFSARMAVPVLGLVYDPKVASYLKELDMPSAG
                     DVHHFRREEAIAAADRLMADYEGSLERLRVKSAALSRAARENERLLLELLERKQ"
     gene            complement(5666..6166)
                     /locus_tag="ACZPAQ_01630"
     CDS             complement(5666..6166)
                     /locus_tag="ACZPAQ_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025582.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HIR0902707.1"
                     /translation="MKKIIDRNGRLFGFISVIDLLVIVVVAVMGFALYTKNTQMAITS
                     TNTADQTITYQILASGIRTYVAEAVREGDQLFDPDRSSGGTLGTITDIQLMPGTKLAE
                     FDDGTVAAATVEDGVDMLITVEGTGLVSESGRYTLNRVYELGVNSSRTFCTKYAEFIG
                     TVTEIF"
     gene            complement(6163..6672)
                     /locus_tag="ACZPAQ_01635"
     CDS             complement(6163..6672)
                     /locus_tag="ACZPAQ_01635"
                     /inference="COORDINATES: protein motif:HMM:NF025582.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4330 domain-containing protein"
                     /protein_id="HIR0902708.1"
                     /translation="MEPNKPKFRFRLNLFDSIVLVLALAVGAFLVWSAIKPAAAPAEG
                     AVSAAGTVQYTIRFQKMIQGASERIKPGDQLVDTVKNYELGTVVSVETVPAQALIQDH
                     EDKAFVLAGIEGYEDALVTVEAPYTEGEDSLLLGGGFKLRVGVTAYVRGSGYMGSGPV
                     VSIERGEQG"
     gene            complement(6659..>7043)
                     /locus_tag="ACZPAQ_01640"
     CDS             complement(6659..>7043)
                     /locus_tag="ACZPAQ_01640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0902709.1"
                     /translation="VFFAGFIERVERALWAMDAQINASTESEGTSLSLLEGMSFGLPA
                     VVSDIGGNPLLIQDGENGLVFPNRDSQALAGCVARLMDDPALLRRLGEGAKRSFEENY
                     TGEIFAKHVEDVYRDVLKGVSHGTK"
BASE COUNT         1254 a         2558 c         2032 g         1199 t
ORIGIN      
        1 ctccactgca ctcacccttt tgcagtacca accaggccct ctcatagcgg aaggtccacc
       61 gcagaaggga aaggccaagg aatgcctcca tgagcaggga acatgaccac agcacaatgc
      121 ttcccgcttt catgacagcg gcaccgcccc cgtcagatcc gggggctttt tcagcacctc
      181 tgcatgagta aaatacaggc ggtcccgcgc gggataccag aaatactcgg cccccaccca
      241 gccctgctcc agcagctcca tcaccaccca gacgtgctcg ccgtcctccg cgacgcacca
      301 gacctggagg gccaggatgg gccccagtcc cagatcctgc ctcacctgat ccatcgtatg
      361 ggccaccagg gtgtccacag tcccgccccc tctttcccag gtgagcagcg cgtcctccgc
      421 cagccccccc tgggtggggc cgcccaggaa gaagaggaag agcagcaccg tcgtcagcca
      481 ggagaggccc cgccaggctc cccctgttcg ctgcgggcgg gccgctgtct tgacccgcac
      541 cgctgtatcg cactccccga agcacaatgg cgtgctccac aggctccgcc cggcccccat
      601 ctccagcagg gtcctggcgt actcccgccg ttcctccggc tccagctcct tcagaacagc
      661 cgcgtcgcat gccagctcca ggtcccgaca gaaatatttg tagcccagcc agatcagcgg
      721 gtcccaccaa tagagaaata cccccatgtt ggcccacccc ttgaaccaga ggtgccccag
      781 cctgatatgc tccagctcgt ggcgcagcac cagggctcgc tgctccgggg acagctcccg
      841 ctggaggcag atcacatatt ccaccgaccc cagccatccc ctgctggtga ggaagctggt
      901 gggcagtccc tccaccagcc ggatctggat cttccgcgtc tcctcctgga ggacatgctt
      961 ggtcagagcc tcctctccgc ccagcagctc ccccctgcgc agcagctcct tcagccgcct
     1021 gtcctgtcgg caggaaaaaa cgatccccag caccaggacg cccagacaca gcagcgcggc
     1081 cgccccggct atcccctccc cgatttccac cggcaccacc gccccgccgg gcagggacag
     1141 atggtacacc cccgccccgt ggtaatccat gggcagatag gcggggattg aggtcgtcca
     1201 tccgccgtcc tttacccgcc cggtgatcag gtcacggaag gtgacgggca ggatgtggac
     1261 caggctgagc aggccgtaaa agctcggact gtacaggctg atccagccca ccacccaaag
     1321 ggccacccgc tgccccgggg agagtatccg caccagcagg gggcgcagca gaaacagggc
     1381 ggcgaacatc attcccactg ccagccccag attgatcatc ataaacatgc ccacaacaaa
     1441 aaatcggccg atcatatccg ctcctctcct ttctctcagg ctcagtccac gggataaaac
     1501 tgagtgcagt ccgccaggtc atggggccgg ccctcctgcc ggagctggat catccccatc
     1561 ttgccgctct cccccagcac gcaccggaac gcccgccaag tgcccacgct ggtctccaga
     1621 tagatccggc ctcctgtgcc ctcccacttc caccagccgg cggcgatgct caccccggag
     1681 agcaggtccc ccttctcctc cagccgccgg atcagctcct ccaccacctt ctccggcgcc
     1741 tcctccatct gcgtcaggtt tcgggagaga tcagtctctg tcactccccc cggcacggcc
     1801 ggcgcccccg caaagaggag gaacaccagg gcggcacaga acagcccggt gaggcggcgc
     1861 acaggggtca ggggacgcca ggccaccgcc gcccgcaccc gcagggctcc gtcgcactcc
     1921 ccaaagcaca gcggggcctc ccacaggcgc cgccccgccc ccagctccac cagggtcttg
     1981 gcgtactcct tccgctgctc cggctccagc tgatccaggg tcctctggtc acaggccagc
     2041 tccatatccc ggcccatata ccgaaatgcc agccacagga tcggattcca ccagtggagc
     2101 agcaggcagg cgcccctgag cccggagaag tacccatggc ggagatagat gtggttcagc
     2161 tcatggcgca gcaccaggga aagctgctct ttgggcagct ctgcctggag gtacaccgaa
     2221 tcccccatga caaagctagt gggcagcccg ggggtgatcc acgcccggat ctttccgggc
     2281 tgcatccaga gctccagccg gtcggccccc agggccacca gctccttctc ctccagctcg
     2341 ctcccctgcc gcccccagca catcagggcg aaggaccggt gacaatacca caggagcagc
     2401 cccaccgcca ccgcggccca caggagggac agggccagca gcggaccgct gttcagctcc
     2461 accgccgcag tttccccgcc gggcaggacc acggggtact cccccggccc gtcgtaggcg
     2521 ggcggcagga aagccggcgc gccgaatatc ccgcccggcc ggggtcccag caggtcccag
     2581 accgttaccg gcagcaccct taccaggctg gtcagccccc acaggttccc aaatatgccg
     2641 atgctccacg ctacatacca gactgccacc cgctgctggg gggtgaggag ccggaggagc
     2701 agagggcgca gcaggagcag gacggccagg acgaccccca gtgccagccc cgcgttcagg
     2761 tgattcaaaa atatggtgag ccacgtgttc agtccgtcca cggtcagtcc tcccaggtgt
     2821 gctggtcgat caggtccttc agctccagtg cctcctccat ggtcagcttc cgcccgctga
     2881 aaaaggcgtt gaggaagggg gccatccccc ccgccttcac cgccagctcc tccccttggg
     2941 ctcggatgac ctgctcccgg gtcagcagcg gggtgaccac cgcgcgctcg tttctggccg
     3001 cccccttccg gcacagcttg cgcagcaccg tataggtggt gctcttggtc cagcccagct
     3061 gctcccggca cagagtcacc agctgggtgg aattcactgg tgcactttcc cacagcacct
     3121 ccatcagccg gtattccccg tcaaacagct tttccaccgt ccgtccccct ttaggtttat
     3181 tctaataaac ctctctttca ctgcaagttt atcgtaataa acctctcttg tcaagagcct
     3241 gatttcccta tcggactccg gagcgggcag cggcacaccc tgcataggcc cgcacaggct
     3301 cgcccgtctc atccctgaaa acgatttacg ggcggccccg cggggccgcc cgtaagcgaa
     3361 aggatctttt ttgttcaagc tgattcggta agccggagcc ccccgtctcc gctcctgccg
     3421 cgcgttccag tcaaaagctc tattgtttcc tctccagcag ctccagcagc aatcgctcgt
     3481 tctcccgggc cgcccggctc agggcggcgg atttcacccg caggcgttcc aggctcccct
     3541 catagtccgc catcaggcgg tccgcggcgg cgatggcctc ctcccggcgg aagtgatgca
     3601 catcaccggc ggagggcatg tccagctcct tcagatagct ggccaccttg ggatcataga
     3661 ccaaccccag cacagggacc gccatccggg cggagaaaat cagggtgtgc agccgcatag
     3721 ccaaacacag cctgccctcc cccagcacgg ccatcagctc cctcggggtg caggtggcgt
     3781 ccagcagatg ggaggggcac tccatagcgc tccgcacctg ggcggtggcc gcccggtccc
     3841 ggcagggctg catcatcagg aacaggatct ccatcccgca ggtccggtag agatggtcgc
     3901 acagggcggc cagctccccg gcgaagctgc ccgtatccgg ccagtcccgc accgagaccg
     3961 ccgcgaaggg agtgccccgc tccagcccgg cggaccggag cagttccccg gcccgctcct
     4021 tgggggcggg atccaggttg aacaccgggt cggccgtgac gtggagatct ggacgctcca
     4081 cccccatctc caccagttcc cgggcggagc tgtggtcccg cagggtcacc agagcggccc
     4141 gctccactgc ccgcttcacc cggcggcggt tggccggctt ctgcaccggc ccgatgccgt
     4201 tggcgtagag catcaccggc ttccccatcc actcggcaca gcgcacgatg gacagatagt
     4261 agaggaggga gcgggtggag gtccggtcct ggagcaggct gcccccgccg gacagcagag
     4321 cgtcgcatcg gcgcagggca ccgaacacct tccagaaacg gaagcggggg atggcattca
     4381 tgccgtagtg ccgctgggtc tcgcgggggt cgttggacag cacggtgacc cccacctccc
     4441 cactggcctc cagaatgctc tggtggatgg cctccaggat ggcgtcgtcc cccgcgttgg
     4501 aaaagccgta gtagccgctc atgaccacct ggtacttccg gcggcgcacc tgctcataca
     4561 tggccagaca gtccgctgcc atccggcgca cggagtaata ctggaagatc acctcccggc
     4621 cgtaggcccc cgccgccgcc ttctgctccg gggagaggga caggacggcg gccatatcgg
     4681 ccagcagacg ctccggagtg gacatgggca gtccccggca gcagaagttg cccagtcggg
     4741 cctcctccag cttctccgga ccgaagaggc cctgataccc ctcgtttccc gccacgatga
     4801 cgggcttggc ggcggacatg gcctccagcg ccgaccggga cacccctacg aagatgtccc
     4861 ccgctgccac gatctgattg atgtcggtgc gtgggccggt catcaccacg caggtgcgtc
     4921 ccagccgggc gttcaccgcc tccgcctgct ctttcagctc ccggaacacg ttgccccctc
     4981 cggcgatgag cagctgcacc ccgggcaccg cccggtccag ctcctccgcg atctggatta
     5041 gctgtcgggc caccagggcc cggtcctcgt ccatccggct cacataggag accactggcc
     5101 tggaccggtc caggccgaac tcctccacca ctgactggcc ggaagtctgt ggagaaaact
     5161 tctctgtatc gatgccgttg atggtcacag agatgtgctc ctccggcacc ccgtattccc
     5221 ggatcagata cgccttgatg tcatcggaga cggcgacggt ccgctgcccc cagttggtga
     5281 ggtagcgcag cccgcccttg gtggtgaaca cccagtgggc ggtggtgacg aaggggaact
     5341 tcatcgttct ctggagggtg ccgcacagga aggcggggat gcgggcgtgg gcgtgcacca
     5401 cgtccggcct ctcagtacgg atgatctcct tcagcagctt tcgggagcgg agcatgcatc
     5461 cggcgtcccg ccggtgcatg gggacctggt agtggcggat gcccgcggcc acgatctcag
     5521 ggacatagac accgccgttg gaggccacca cgatgtcgtg tccctccgcc ttcagctcct
     5581 tgtccagctc tacgatatgg gtctccgctc cgccgatatc caggcccata gtggccatca
     5641 ggatcttcat gtctgccgcc cctttttaaa aaatctctgt cactgtgccg atgaactccg
     5701 cgtattttgt gcagaaggtc cgggaggagt tgacccccag ctcatacacc cgattcaggg
     5761 tatagcgtcc cgactcgctc accaggccgg tgccctctac ggtgatcagc atgtccacgc
     5821 catcctccac cgtggcggcc gccacggtgc cgtcgtcaaa ctcggccagc ttggtgccgg
     5881 gcatgagctg gatgtcggtg atggtgccca gggtgccgcc gctgctccgg tccgggtcaa
     5941 agagctggtc gccctcccgc actgcttcgg ccacataggt ccgtatccca ctggccagga
     6001 tctggtaggt gatggtctgg tccgccgtgt tggtgctggt gatggccatc tgggtgttct
     6061 tggtgtacag ggcaaagccc atcaccgcca ccactacgat gaccagcagg tcgatgacgc
     6121 tgatgaagcc gaacaggcgg ccgttgcggt cgatgatctt tttcatccct gctcccccct
     6181 ctcaatggaa accacgggcc cgctgcccat atagccggag ccccggacat aggcggtcac
     6241 gcccacccgc agcttgaagc ctccgcccag caggagggag tcctctccct cggtataggg
     6301 ggcctccact gtcaccaaag cgtcctcgta accctcgatc ccagcgagga caaaggcctt
     6361 gtcctcgtga tcctggatca gagcctgggc cggcactgtc tccacagaca ccactgtgcc
     6421 cagctcgtag ttcttcacgg tgtccaccag ctggtccccg ggcttgatcc gctcgctggc
     6481 tccctggatc atcttctgaa atcggatggt gtactgcacc gtccctgccg cggaaacggc
     6541 cccttccgcc ggtgcggccg ccggctttat ggcgctccac accaggaatg cccccaccgc
     6601 cagggccagt acaaggacga tgctgtcaaa caggttcagt cggaagcgga atttcggctt
     6661 atttggttcc atgagatact cctttcagga cgtcccggta gacgtcctcc acatgcttgg
     6721 caaagatctc gccggtatag ttttcttcaa aggaccgttt ggccccctct cccagcctgc
     6781 gcagcagggc ggggtcatcc atcagccgtg ccacacagcc ggccagggcc tggctgtccc
     6841 ggttggggaa gaccaggccg ttctctccgt cctggatcag cagggggttg cccccgatat
     6901 cgctgaccac ggcgggcagg ccgaagctca tgccctccag cagggagagg ctggtcccct
     6961 cgctctcggt ggaggcgttg atctgagcgt ccatggccca cagggcccgc tccacccgct
     7021 caataaagcc ggcaaaaaac act
//
LOCUS       DBMDSJ010000036         6975 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3236_length_6975_cov_1.700902, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000036 DBMDSJ010000000
VERSION     DBMDSJ010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3236_length_6975_cov_1.700902"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(197..967)
                     /gene="recO"
                     /locus_tag="ACZPAQ_01645"
     CDS             complement(197..967)
                     /gene="recO"
                     /locus_tag="ACZPAQ_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR0902710.1"
                     /translation="MHMRTKALVLRAVDYKESDKILTLLTQESGKLTASARGCRKKGS
                     PVAAGVQLLAWSDLVLYEYRGRWTVKEAAVEREFLGLRRDVERLALACYFAEAAEALA
                     VEDVPAPELLSLVLNSLYALDQMPQKPLALVKAAFELRLMCAAGYEPILDGCAVCGAE
                     RPEEPRFHLREGALHCAKCRGGVGEGISMPVSPPVLAAMRHVVCGDARRLFSFRLDSG
                     ALEELAGVSEAYLLTQLERGFRTLDFYKQMTLPAGPDR"
     gene            complement(1027..1206)
                     /locus_tag="ACZPAQ_01650"
     CDS             complement(1027..1206)
                     /locus_tag="ACZPAQ_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902711.1"
                     /translation="MEQSGFWNFFFATGLPQAYLAARLKEGEEVVFPPMPPPGAPGGG
                     TLPPSVPGMMDKKDM"
     gene            complement(1292..2188)
                     /gene="era"
                     /locus_tag="ACZPAQ_01655"
     CDS             complement(1292..2188)
                     /gene="era"
                     /locus_tag="ACZPAQ_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR0902712.1"
                     /translation="MSIKKSGMISIVGRPNVGKSTLTNALVGEKVAIVSNKPQTTRNR
                     ICAILNRGESQFVFLDTPGLHKARTRLGDYMVNVVKESVADVDAVMLLVEPIPNVGGP
                     EAELIARIKTLGCPSVLVINKVDTLEHKEELLEVIQTYGQAHEFTAVVPVSARTGEGV
                     EELLDVLDGFLPQGPQLFPEDMVTDQPERQMMAEILREKLLLCLDKEIPHGTAVEITK
                     FSERDDEVIEIDATIYCEKNSHKGIIIGKGGAMLKKVSSLARQDMERFMGTKVYLQTW
                     VKVKENWRDNVNLIRNFGYRDE"
     gene            complement(2201..2506)
                     /locus_tag="ACZPAQ_01660"
     CDS             complement(2201..2506)
                     /locus_tag="ACZPAQ_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902713.1"
                     /translation="MLANFFVLWLLLAVGGGFLLFFGGLLASWIGEEMVCFLLATALL
                     ALAGALIIQLRERLQQTRRRLEEETDRLRRMEEKLDQLLERGTDHKAESAHTAGKES"
     gene            complement(2565..3623)
                     /locus_tag="ACZPAQ_01665"
     CDS             complement(2565..3623)
                     /locus_tag="ACZPAQ_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR0902714.1"
                     /translation="MNIWQKRAPGVLLCLAVALLAWFLVELAPALEIVGSAVLAILFG
                     MVLCAVMKNKDKVKDGVAFTSKKVLQYAVILLGFGLNLRKIGEVGLTSLPIILSTIAT
                     SLIVAFLMSRALHMPGKIATLIGVGSSICGGSAIAATAPVIEADDEEIAQSISVIFLF
                     NVLAALIFPTLGGAIGLSDTGFGLFAGTAVNDTSSVTAAASTWDTLHNTGGAVLEYAT
                     IVKLTRTLAIIPITLALALFRVRQAEKAGGKSGVKVSAKSVFPMFILYFVLASVITTV
                     VTAVLSGPALAGANELFSLLKTLSKFFIVMAMAAIGINTDVVKLVKTGGKPILLGFTC
                     WAAITCVSLGMQHLLGIW"
     gene            complement(3800..4522)
                     /gene="ybeY"
                     /locus_tag="ACZPAQ_01670"
     CDS             complement(3800..4522)
                     /gene="ybeY"
                     /locus_tag="ACZPAQ_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR0902715.1"
                     /translation="MIFNERLRRLRKERGLTQVQTAEHLSIAWRNYQRLEKDGNAPSF
                     LTFVKAAEFFGVSLDYLAGRTDAREVNRGTCPPAGPEHRIILEAEVEAPDTVEPLLRR
                     VISAALEAEGMDLPCEINVLLTDDEGIHQVNLDMRGVDRPTDVLSFPMFDLSPGEKPG
                     EEHEDPDTGLVPLGDMCISLERAAAQAEEYGHSVERELSYLAVHSVLHLLGYDHMDEG
                     PMKAQMRRREEHILEGLGIRRG"
     gene            complement(4644..5591)
                     /locus_tag="ACZPAQ_01675"
     CDS             complement(4644..5591)
                     /locus_tag="ACZPAQ_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR0902716.1"
                     /translation="MTEQSISLERLEETINVFGSFDENIRIIEGEMEVSVVSRDSMLK
                     VSGENAENVMYAVKAIEALMSLSSRGEAINEQNVRYIIQLVRSGNESQISQLAGDVLC
                     VTAKGRPIKAKTLGQKKYVEAIKKTYLAVAAAVAAFRAKEVNRIILTRPAVEAGERLG
                     FLPGDLQSKVDPYLRPLYDALFDMLGAETYNKYLERGNIEVAPLAYMRGRTLDDSFII
                     LDEAQNTSREQMKMFLTRLGFGSKIVITGDITQIDLPADKVSGLREAMRVLKGVEDIS
                     ICRLTESDVVRHVLVRRIIKAYEEDEERRGPKKDRRERP"
     gene            complement(5614..6846)
                     /locus_tag="ACZPAQ_01680"
     CDS             complement(5614..6846)
                     /locus_tag="ACZPAQ_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR0902717.1"
                     /translation="MQWIVNRLRGELRVVARGPFPERLMNVCAQNGISFWALEWRDGH
                     TLSMTVRQEDRKQLWKLAERVGCTVETEGSRGLPAFLARFRTRYAFLAGFALSLAAVC
                     ILSSFVLTIQVTGNETVPTGVILSELRRLGVRPGAYGPALERRQIAEEALLSLDELSW
                     MSLNLHGTRLEVQVREAVQPPELTQEEGHYHVVSKADGIVTRVETMDGEAAVKEGDTV
                     TEGEILISGTVSMEPPMYSDQPVRYYQTHARGRVEARTWRTLTASIPLTAQVKAYTGE
                     EKSRFTLTVLGYPVEFYQNAGISWPWYDKISTVYQAGLPGGLTLPLSWTVERYRAYEP
                     VQTEVDRTAAQTLLEERLLAQLKEQIGEEGRVVSTYYTARAADSWLEVTLTAECREEI
                     GREVPAAPDAGDSAPEQP"
BASE COUNT         1131 a         2449 c         2111 g         1284 t
ORIGIN      
        1 gttccccgga agagtcccct cctccggaaa caggcacttt attatattag cacaagcggg
       61 gagggaattc cagtcttttt cctccaaact tatgtcaact tctcgtcttt tcccctccag
      121 gcaggagagg ccccgtccgg cccaggctgg acggatggaa aaaagacgct gtccgtccga
      181 acggacagcg tccctctcac ctgtccggcc cggccggcag ggtcatctgc ttatagaagt
      241 ccagggtgcg gaagccccgt tccagctggg tgagcagata cgcctcgctc accccggcca
      301 gctcctccag ggccccgctg tccagccgga aggaaaacag ccgccgggcg tccccgcaaa
      361 ccacatgccg catagcggcc aggacggggg gcgagaccgg catggagatc ccctccccca
      421 caccgcctcg gcatttggcg cagtggagcg ccccctcccg caggtggaag cggggctcct
      481 ccggccgttc cgcgccgcag acggcgcagc cgtccaggat gggctcatag cccgcagcgc
      541 acatcagccg cagctcaaag gccgccttga ccagggccag gggcttctgg ggcatctggt
      601 ccagggcgta caggctgttg agcaccaggg agagcagctc cggcgccggc acgtcctcca
      661 ccgccagcgc ctccgccgcc tcggcgaagt agcaggccag ggccagccgc tccacatccc
      721 ggcgcagccc cagaaactcc cgctccaccg ccgcctcctt cacggtccag cggccccggt
      781 actcatacag caccaggtcc gaccaggcca gcagctggac cccggcggcc accgggctcc
      841 ccttcttacg gcagccccgg gccgaggcgg tgagcttccc gctctcctgg gtcagcaggg
      901 tcaggatctt gtccgactcc ttgtaatcca ccgcacggag gaccagcgct ttggtgcgca
      961 tatgcatctc gtccgctcct ctatgtatct ccggccggtc cgccgagggc gggccggccg
     1021 ggcagttcac atatcctttt tgtccatcat gcccggcaca gacgggggca gagtcccgcc
     1081 ccccggcgct ccgggagggg gcatgggagg gaagaccact tcctccccct ccttcagccg
     1141 ggccgccaga taggcctggg gcagtccggt ggcaaaaaag aagttccaaa atccgctctg
     1201 ttccatctgt cttgccctcc tgtctctgct gcttaggctg gcatctccag tggaaaacat
     1261 gcgcggtaat ttttaggccc ggccttttcc ctcactcatc ccggtatccg aagttacgga
     1321 tcagattcac gttgtcgcgc caattttctt ttactttcac ccaggtctgg aggtacacct
     1381 tggtccccat gaatcgctcc atgtcctgcc gggccaggga ggacaccttt ttcagcatgg
     1441 ctcccccctt gccgatgatg atccccttat ggctgttctt ctcgcagtag atggtggcgt
     1501 cgatctcgat gacctcgtca tcccgctcgg aaaacttggt gatctccacg gcggtgccgt
     1561 gggggatctc cttgtccagg cagagcagca gcttctcccg caggatctcc gccatcatct
     1621 ggcgctccgg ctgatcggtg accatgtcct ccgggaagag ctggggcccc tggggcagaa
     1681 acccgtccag tacgtccagc agctcctcca ccccctcgcc ggtcctggcc gagacaggga
     1741 ccacggcggt gaactcatgg gcctgcccgt aggtctggat gacctccagc agctcctcct
     1801 tgtgctccag ggtgtccacc ttgttgatga ccagcaccga ggggcagccc agggtcttga
     1861 tgcgggcgat gagctccgcc tccgggccgc cgacgttggg gatgggctcc accagcagca
     1921 tgaccgcgtc cacgtcggcc acgctctcct tcaccacgtt caccatgtag tcccccaggc
     1981 gggtgcgtgc cttgtgcagg cccggcgtat ccagaaagac gaactggctc tccccccggt
     2041 tcagaatggc acagatccgg ttccgggtgg tctggggctt gttggagacg atggccacct
     2101 tctcccccac cagggcattg gtcagggtgg acttgcccac gttggggcgg cctacaatag
     2161 agatcattcc cgattttttg atggacatag cgtttgttcc tcaactttct tttccggcgg
     2221 tgtgagcact ttccgcctta tgatccgttc cccgctccag cagctggtcc agcttctcct
     2281 ccatccggcg gagccggtct gtctcctctt ccagccggcg ccgggtctgc tggagccgct
     2341 cccgcagctg tatgatcagc gccccggcca gggccaggag ggcggtggcc agcagaaagc
     2401 agaccatttc ctccccgatc caggaggcca gcagcccgcc gaaaaagagc agaaatccgc
     2461 cgccaacagc cagcaggagc cacaggacaa aaaagttggc gagcatcgtt ttccctccct
     2521 gtttttcatg gagcgccgcc cgctgacggg cgggcgcagg tcctttacca gatgcccagc
     2581 aggtgctgca tgcccaggct gacgcaggtg atggccgccc agcaggtgaa gcccagcagg
     2641 atgggcttgc ctcccgtctt caccagcttg accacgtcgg tgttgatgcc gatggccgcc
     2701 atggccatga cgataaagaa cttgctcagg gtcttgagca gggagaaaag ctcattggcc
     2761 cccgccaggg cgggaccgga gagcacggcg gtgaccaccg tagtgatgac ggaggccagc
     2821 acaaagtaca ggataaacat ggggaacacc gacttggcgg acaccttcac gccgctcttc
     2881 ccgccggcct tctccgcctg gcgcacccgg aagagggcca gcgccagggt gatggggatg
     2941 atggccaggg tgcgggtcag cttgacgatg gtggcatact ccagcaccgc gccgccggtg
     3001 ttgtgcaggg tgtcccaggt ggaggcggcc gccgtgacgg aggaggtgtc gttcaccgca
     3061 gtgccggcga acaggccgaa gccggtgtcg ctcagcccga tggccccgcc cagggtgggg
     3121 aagatcaggg ccgccagcac attgaacagg aagatcaccg agatggactg ggcgatctcc
     3181 tcatcgtccg cctcgatcac cggggcggtg gccgcgatgg cggagccccc gcagatggag
     3241 gagcccacgc cgatgagggt ggcgatcttc ccgggcatat gcagggcccg gctcatgagg
     3301 aaggccacga tgagggaggt ggcgatggtg gacaggatga tgggcaggga ggtgagcccc
     3361 acctcaccga tcttccgcag gttcaggccg aagcccagca ggatgaccgc gtactggagc
     3421 acctttttgg aggtgaaggc caccccgtcc ttcaccttgt ccttgttttt catcaccgcg
     3481 cacagcacca tgccgaacag gatggccagc accgcgctgc ccacgatctc cagagccggg
     3541 gccagctcca ccaaaaacca ggccagcagt gccaccgcca ggcacagcag cactccgggt
     3601 gcccttttct gccaaatatt catctctttc tctcctagat ctctctttct gtctcccgcg
     3661 gccggccgcc ggcctctccg gcatttcggg ccggtgcgcc tctgcgggac tgcttattgt
     3721 accacaccgg cccgccccgc gcaatcccct ccgggcccgg cgggacattt tctcccgctt
     3781 ccccgcggga cgtggcctct catccccggc ggatgcccag cccctccagg atgtgctcct
     3841 cccgccggcg catctgggcc ttcatggggc cttcgtccat gtggtcatag cccagcagat
     3901 ggagcacaga atggaccgcc agatagctca gctcccgctc cacactgtgg ccgtactcct
     3961 ccgcctgggc ggcggcccgc tccagagaga tgcacatatc ccccaggggc accagcccgg
     4021 tgtccgggtc ctcgtgctct tcccccggct tctcccccgg agagaggtcg aacatgggga
     4081 aggagagcac gtcggtgggc ctgtccaccc cccgcatgtc cagattcacc tgatggatgc
     4141 cctcgtcgtc ggtgaggagc acgttgatct cacagggcag gtccatcccc tccgcctcca
     4201 gggcggcgga gatgacccgg cggagcaggg gctccacagt gtccggggcc tccacctccg
     4261 cctccaggat gatccggtgc tccggccccg ccggaggaca tgtcccccgg ttgacctccc
     4321 gcgcatcggt ccggcccgcc agatagtcca gggagacgcc gaagaactcc gccgccttca
     4381 caaaggtgag aaagctgggg gcgttcccat ccttctccag gcgctggtaa ttccgccatg
     4441 cgatggacag gtgctctgcc gtctgcacct gggtcagacc gcgctccttc cgcaggcgcc
     4501 tcagccgttc attgaatatc atttgtaccc gcctccattg atggatatcg tatctcttcc
     4561 tcccgctgca tcgtttctcc cggcgggcgc gcgcaccctg cgcgggggct cctcagggcg
     4621 ggacctcttt gggatgcccg aggtcagggc cgctcccgcc tgtccttttt gggaccgcgg
     4681 cgctcctcgt cctcctcata ggccttgatg atccgccgga ccagcacgtg gcggaccacg
     4741 tcgctctccg tcaggcggca gatggagatg tcctccaccc ccttgagcac ccgcatagcc
     4801 tcccgcaggc cggagacctt gtccgcgggc aggtcgatct gggtgatgtc gccggtgatg
     4861 acgatcttgg agccgaagcc aaggcgggtg aggaacatct tcatctgttc ccgggaggtg
     4921 ttctgggcct cgtccaggat gatgaagctg tcgtccaagg tgcggccccg catataggcc
     4981 aggggggcca cctcgatgtt cccccgctcc agatacttgt tgtaggtctc cgcgcccagc
     5041 atgtcgaaca gggcgtcgta caggggccgc agataggggt ccaccttgga ctggagatcc
     5101 ccaggcagga agcccagccg ctcacccgcc tccactgcgg gtcgggtgag gatgatgcgg
     5161 ttgacctcct tggcccggaa ggcggccacc gccgccgcca ccgccagata ggtcttcttg
     5221 atggcctcca catacttctt ctgtcccagg gtcttggcct tgatgggccg ccccttggcg
     5281 gtgacgcaca gcacgtcccc cgccagctgg ctgatctggc tctcgttgcc gctgcggacc
     5341 agctggatga tatagcgcac gttctgctcg ttgatggcct ccccccggga ggagagggac
     5401 atgagggcct cgatggcctt gaccgcgtac atcacgtttt ccgcgttctc gccgctcacc
     5461 ttcagcatgg agtcccggct caccaccgag acctccatct cgccctcaat gatacggatg
     5521 ttctcgtcaa aggagccgaa cacgttgatg gtctcctcca gccgctccag gctgatgctc
     5581 tgttctgtca tttgcaggaa atctccttac ttgttatggt tgttccgggg cgctgtctcc
     5641 ggcgtccggt gccgcgggca cctcccggcc gatctcctcc cggcactcgg cggtgagggt
     5701 gacctccagc cagctgtccg ccgcccgggc ggtgtagtag gtggagacca cccggccctc
     5761 ctccccgatc tgctccttca gctgcgccag caggcgctcc tccaacagag tctgggcggc
     5821 ggtgcggtcc acctccgtct ggacgggctc ataggcccgg taccgctcca ctgtccagga
     5881 cagggggagc gtgagcccgc cgggcagccc ggcctggtac actgtactta ttttatcata
     5941 ccagggccag gaaatgccag cgttttgata aaattccacg ggatatccca ggaccgtcag
     6001 ggtaaagcgg cttttttcct ctcctgtgta cgccttgacc tgggcggtga gcgggatgga
     6061 cgcggtgagg gtgcgccagg tcctggcttc cacccggccc cgggcgtggg tctggtagta
     6121 gcgcaccggc tggtcgctgt acatgggggg ctccatgctc accgtgccgc tgatgaggat
     6181 ctccccctcc gtcactgtat ccccctcctt caccgccgcc tccccgtcca tggtctccac
     6241 ccgggtcacg atgccgtccg cctttgacac cacgtggtag tggccctcct cctgggtcag
     6301 ctcggggggc tggaccgcct cccgcacctg cacctccagc cgggtgccgt ggaggttcag
     6361 ggacatccag gacagctcat ccagggacag cagggcctcc tcggcgatct gccgccgctc
     6421 cagggccgga ccgtatgccc cgggccgcac ccccagccgc cgcagctcgc tgaggatcac
     6481 cccagtgggg acggtctcgt tccctgtcac ctggatggtg agcacgaagc tggacaggat
     6541 gcacaccgct gccagggaca gtgcgaaccc cgccaggaag gcataccggg tgcggaagcg
     6601 ggccaaaaag gcgggcaggc cccggctccc ctccgtctcc acagtgcagc ccacccgctc
     6661 cgccagcttc cacagctgct tccggtcctc ctgccgcacc gtcatggaca gggtgtgccc
     6721 atcccgccac tccagggccc agaatgagat cccattctgg gcgcacacgt tcatcaggcg
     6781 ctccgggaag ggcccccggg ccaccacccg cagctccccc cgcagccggt tcacgatcca
     6841 ctgcacacgc gttccctccc ctcaggccag ctggatgccg gcgatgcggc cggtgatgag
     6901 cagctccagc ccggtcatgg cccgcagctc cagcccctgg ccggtgatct ggatgacgaa
     6961 ggccccgccg ctgac
//
LOCUS       DBMDSJ010000037         6916 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3263_length_6916_cov_1.816728, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000037 DBMDSJ010000000
VERSION     DBMDSJ010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_6916_cov_1.816728"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="ACZPAQ_01685"
     CDS             complement(<1..222)
                     /locus_tag="ACZPAQ_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR0902718.1"
                     /translation="MDSWHSRPQADLFQELDARPGGLTRRQAEERLARYGPNQLEPPR
                     RPGLLSRVMAQMKDPMILVLLGAAGLSPGR"
     gene            388..750
                     /locus_tag="ACZPAQ_01690"
     CDS             388..750
                     /locus_tag="ACZPAQ_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR0902719.1"
                     /translation="MWKLTDSEWKVLEALWAGGPQHLGQILGALAPETGWSRTTVHTY
                     LTRMAAKGLVSMDQSSPRRYAAIPSRETCAARERRDLVDRVYDGSAGQLVAAFVKDGS
                     LSPQEREELRRLLDEMEV"
     gene            753..2843
                     /locus_tag="ACZPAQ_01695"
     CDS             753..2843
                     /locus_tag="ACZPAQ_01695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR0902720.1"
                     /translation="MFDLWSFLHQTAAASMTALFVLVLQHIFRDKLSPQWQYAVWMVV
                     VLRLILPVGWLGRSAIADLTGLGEALRTWVELGLDSAWSSPWTPSLPLAGIPIPPAGG
                     LPRSVTDWLFLLYLAGVLLSALWLAAGGLRLRRSLGEAVPVAGARLEAVAALAERFGL
                     SMPRRVVESRAASTPFLVGVVRPVLVLPMGWAPDRKVILHELIHLKQRDVAAGWVTAL
                     FRCVHWCNPFLWRIFDRIDNQREQRCDQLVLERLEGEDRRDYGRVLLSMADDGRRRFP
                     GATTMANGGQNIKARIQAITRFKAFPRGMGLVSVCMVVTLTLSLVVGLPVSAADPLPG
                     AVRLLGPVGSLSYALSHRPATVGGALDAYSKGIYAQYHDVQDCYAYQAMTLPQEQLPD
                     LVEEWRQTDRAGWSVLPGTDPRSASYRTGPIFRGLSYDGEGGYWTQMYFFRDEGGPRE
                     GTERSIGYRRHTLHLQRREDGTWTVSPLSVQTGRLASPFEWEQIRLTSYDNLPFPSQE
                     PPVFTWHGEADGVELELWPEAELRVQDAYIEGSAVKKLDLNGFGSSIHTDAPDLDARF
                     TSVVGGMVLRLTNHTDQPKTMTLDVTQYWDEGVSVLTGTDAPEDGQQEDPVFGLLSQV
                     QNSCYTGYDVSGLEVELAPGESRVLYWGSRGKGYANRIPAADCVMADWLEAVYTDSQG
                     NEVPIPLEREVHLD"
     gene            2840..3451
                     /locus_tag="ACZPAQ_01700"
     CDS             2840..3451
                     /locus_tag="ACZPAQ_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902721.1"
                     /translation="MNSKLIRGVQYAAWAYFFLYLDINLNRFSLLPNAVGWYLLSRAV
                     TALEEEHPDLRLLGPLTFPLGLWALKQYAFLPPAWDLSQFSWLLSWLALAVELTTLYF
                     HFQFLTDLADIAARHAGETGRDFSPALLRARTVVTVLSTAASVLFYLGVDSSGPLSSF
                     SIALTLFLLVVLVVQILCTTVLLFRFSSALRRAGPVLPEGPGI"
     gene            complement(3513..4799)
                     /locus_tag="ACZPAQ_01705"
     CDS             complement(3513..4799)
                     /locus_tag="ACZPAQ_01705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR0902722.1"
                     /translation="MRSMKRAGALTLAALILAFSLTACGKDGGSSGSGASSSAAGSTS
                     SSEPITVDLDAVTDVFLTTAGIPGDTVVAQVGDVDITAAELLYWVAYSADSMLSYYST
                     YFGITELPWDTEDASGVTLTQGTLDNALRTAALYALIPGIAEEEGVTLSQDFQDTFAD
                     QLATMTEAMGGEDVMAMYLWQYPLTPELYTQLCESEDLNGQLQDKYFGENGTMKPTDA
                     DLLSYIQNDRKLYSVKHILLLTQDPETGEPLDEAAAAEKKAQAEDLLRQLRESSDPAA
                     LFDQLMNDYSEDTGLATNPDGYQAVEAGQMVPEFEEASLALEPGEISDIVESDYGYHI
                     ILRLPLEVEPADYQADFVQEKMTQLQQGWLDEHEIVTNDAFDQLDVPAFYETVTALRQ
                     AVSTRMEELSAQEDASASAGTSASASAPAASGSAQN"
     gene            complement(4940..>6916)
                     /gene="mfd"
                     /locus_tag="ACZPAQ_01710"
     CDS             complement(4940..>6916)
                     /gene="mfd"
                     /locus_tag="ACZPAQ_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR0902723.1"
                     /translation="HGVGRYMGMVKLPADGVEKDYVKIAYAGSDVLYVPATQLDLVSK
                     YIGGGEDAAETKKLSRLGGTDWEKAKSKAKKAVKDLAKGLIQLYAQRQRQPGFAFSPD
                     SPWQREFEEEFEYAETEDQLRCVEEIKRDMERPVPMDRLLCGDVGYGKTEVAFRAVMK
                     CVLDGKQAAVLVPTTVLARQHFLNALRRFAKYPVTIDVVSRFRTSAQMKETLRRVESG
                     EVDILIGTHRLFNRDVRFKDLGLLVVDEEQRFGVQHKEKLKENFRQVDVLTLSATPIP
                     RTLNMALSGIRDMSTLEEPPADRQPVQTYVLEHDWGVLGDAMRRELERGGQVYYLHNR
                     VETIDRTASRIQAMLGEDAAVGVAHGKMTQEAIDEVMSRMTDGELNVLVCTTIIETGI
                     DLPNANTLIIEDADRLGLAQLHQIRGRVGRSSRRAYAYLTYRRGKVLTEVAAKRLSAI
                     REFAEFGSGFKIAMRDLEIRGAGNVLGPEQSGFLLSVGYDMYLKLLEEAVLQEQGQPV
                     PPKTECAADLSVAASVPDRYVPSPEQRMDLYRRIAAIRSEAEADDLVDELIDRYGEPP
                     RTVNNLISVALLRAAAAEAGITDIAQKQGQLVFTLARFSLEQFSALCAMDRYQNRLLL
                     TPGEVPRFTFRLKKGEDALRAARQVVDDFAGAEG"
BASE COUNT         1027 a         2394 c         2211 g         1284 t
ORIGIN      
        1 tctcccgggt gacagccccg ccgcccccag cagcaccaag atcatggggt ccttcatctg
       61 ggccatcacc cgggagagca gccccggccg cctggggggc tccagctgat tcggcccgta
      121 ccgggccagc cgctcctccg cctgccgccg ggtcagtccc ccgggccgcg cgtccagttc
      181 ctgaaaaaga tcggcctgcg gccggctgtg ccagctgtcc atgacctcac gctcccttcc
      241 tttgtaccgc tcagtataga ccccggcctg tccatttttt gcagaatcca gggggctggg
      301 acaggctttt tcccgacaag gcttgacaag ttcctcctac acatgtagaa tcatctcaga
      361 aattctacat gtgtaggagg ggtctttatg tggaagctga cggacagcga gtggaaggtg
      421 ctggaggccc tctgggccgg cgggccccag catctggggc agatcctggg cgccctggcg
      481 ccggagaccg gctggagccg caccaccgtg cacacctacc tcacccggat ggccgctaag
      541 gggctggtat ccatggacca gtcctccccc cggcggtacg ccgctatccc cagccgggag
      601 acgtgcgccg cccgggagcg gcgggatctg gtggaccggg tctatgacgg ctcggcgggc
      661 cagctggtgg ccgcctttgt gaaggacggc agcctctccc cccaggagcg ggaggagctg
      721 cgccgcctgc tggacgagat ggaggtgtag ccgtgttcga tctctggtcc ttcctccatc
      781 agacggcggc ggccagcatg accgccctct tcgtgctcgt gctccagcac atcttccggg
      841 acaagctctc cccccagtgg cagtacgcgg tctggatggt ggtggtgctc cggctgatcc
      901 tgcctgtggg ctggctgggc cgctctgcca tagccgacct gacggggctg ggcgaggccc
      961 tgcggacttg ggtcgagctg gggctggact ccgcctggtc ctccccctgg accccctccc
     1021 tgcccctggc gggcatcccc atccccccgg cgggcggact gccccgctct gtcactgact
     1081 ggctgttcct cctctatctg gcgggggtcc tcctgtccgc cctgtggctg gcggcgggcg
     1141 ggctgcggct gcgccgctcc ctgggggagg ccgtgccggt ggccggagcc cggctggagg
     1201 ccgtcgccgc cctggcggag cgcttcggtc tgtccatgcc ccggcgggtg gtggagagcc
     1261 gcgccgcctc cacccccttc ctggtggggg tggtccgtcc ggtgctggtg ctccccatgg
     1321 gctgggcccc ggaccgcaag gtgatccttc acgagctgat ccatttgaaa cagcgggacg
     1381 tggccgccgg gtgggtcacc gccctgttcc ggtgcgtcca ctggtgcaac cccttcctgt
     1441 ggcgcatctt tgaccgcatc gacaaccagc gggagcagcg ctgtgaccag ctggtgctgg
     1501 agcggctgga gggggaggac cgccgggact acggccgggt cctgctgtcc atggcggatg
     1561 acggccggcg ccgcttcccc ggcgccacca ccatggccaa cggaggacag aacatcaagg
     1621 cacgcatcca ggccatcacc cggttcaagg cctttccccg gggaatggga ctggtctccg
     1681 tctgcatggt ggtgacgctg accctctccc tggtagtggg cctgccggtc tccgctgccg
     1741 atcccctccc cggcgctgtc cgcctgctgg gccccgtggg cagtctctcc tacgccctgt
     1801 cccaccggcc cgccaccgta gggggcgccc tggacgcata cagcaagggc atctacgccc
     1861 agtatcacga cgttcaggac tgttacgcct accaggccat gaccctcccc caggagcagc
     1921 tccccgacct ggtggaggag tggcggcaga cggaccgggc gggctggagc gtgctcccgg
     1981 ggaccgatcc ccgatcggcg tcctaccgca ccggccccat cttccgggga ctctcctatg
     2041 atggggaggg gggttactgg acccagatgt acttcttccg ggacgagggc gggccgcggg
     2101 agggcactga gcgctccatc ggctaccgcc gccacaccct ccacctccag cgccgggagg
     2161 acggcacctg gacggtctcg cccctgtcgg tacaaaccgg ccggctggcc tccccctttg
     2221 agtgggaaca gatacggctc acttcttacg acaaccttcc cttccccagc caggagcccc
     2281 ccgttttcac ctggcacgga gaggcggatg gggtggaatt ggagctatgg cccgaggcag
     2341 agctccgggt ccaagacgcc tatatagagg gctctgctgt caaaaagctg gatctgaatg
     2401 gcttcggcag ctcgatccat accgatgccc cagatctgga tgcccggttt accagtgttg
     2461 tgggcggaat ggtgcttcga ctcaccaatc ataccgacca gcccaaaacc atgaccctgg
     2521 acgtcactca gtactgggat gagggggtct ccgtcctgac cggaacggat gccccggagg
     2581 acggccagca ggaggacccg gtgttcggtc tgctgtctca agtccagaac agctgttata
     2641 ccggctatga tgtgtccggt ctggaggtgg agctggcccc cggggaaagc cgggttctgt
     2701 actggggcag ccgcggcaag gggtatgcca accgcatccc cgccgcggac tgcgtcatgg
     2761 cggactggct ggaggccgtc tatacggaca gccaggggaa cgaggtcccc atcccactgg
     2821 agcgggaggt gcatctggat tgaactcaaa actgatccgc ggggtccagt acgccgcctg
     2881 ggcctacttc ttcctctatc tggacatcaa ccttaaccgc ttctccctcc tccccaatgc
     2941 ggtggggtgg tatctgctga gccgggccgt caccgccctg gaggaggagc atccagacct
     3001 gcggctcctg ggccccctca ccttccccct gggcctctgg gcgctgaagc agtatgcctt
     3061 cctcccccca gcctgggacc tgtcccagtt ctcctggctg ctgagctggc tggcgctggc
     3121 ggtggagctc accaccctgt atttccactt ccagttcctc acagacctgg cggacatcgc
     3181 cgcccgccac gccggagaga cgggccgtga cttctccccc gccctgctcc gggcccgcac
     3241 ggtggtcacc gtcctctcca ccgccgcctc cgtcctgttt tacctggggg tggacagctc
     3301 cggccccctg agttccttct ccatcgcgct gaccctgttc ctgctggtgg tgctggtggt
     3361 ccagatccta tgcaccaccg ttctcctgtt tcgcttctcc tccgctctcc ggcgggcagg
     3421 ccccgttttg ccggagggcc ccggtatata aaaacctccg ccctgccccc acatgggggc
     3481 agggcggagg tttttatacg ctgtgaaggg tctcagttct gcgcgctgcc gctggcggcg
     3541 ggggcgctgg cggaggcgga ggtcccggcg gatgcggagg cgtcctcctg ggcgctcagc
     3601 tcctccatcc gggtgctcac cgcctgccgc agggcggtga ccgtctcata gaaggcgggc
     3661 acgtccagct ggtcaaaggc atcgttggta acgatctcat gctcatccag ccagccctgc
     3721 tggagctggg tcatcttctc ctgcacaaag tccgcctgat agtcggccgg ctccacctcc
     3781 aggggaaggc gcaggatgat gtggtagccg taatcgctct ccacgatgtc actgatctcg
     3841 ccgggctcca gggccaggga cgcctcctcg aactcgggga ccatctgacc tgcctccacg
     3901 gcctgatagc cgtccgggtt ggtggccagt ccggtgtcct cgctgtaatc gttcatcagc
     3961 tggtcgaaca gggcggcggg gtcgctgctc tcccgcagct ggcggagcag atcctccgcc
     4021 tgagccttct tctccgccgc ggcagcctcg tccaggggct ctccggtctc cgggtcctgg
     4081 gtcagcagca ggatgtgctt gacggagtac agcttccggt cgttctggat gtaggagagc
     4141 aggtcggcgt cggtgggctt catggtgccg ttctctccaa aatacttatc ctgaagctgg
     4201 ccgttcaggt cctcgctctc acacagctgg gtgtagagct caggagtcag gggatactgc
     4261 cacaggtaca tggccatcac gtcctcgccc cccatggcct cagtcatagt ggccagctga
     4321 tcggcaaagg tgtcctggaa gtcctgggac agggtcacgc cctcctcctc cgctatcccg
     4381 gggatcaggg cgtacagggc cgcagtcctc agcgcgttgt ccaaagtccc ctgggtcagg
     4441 gtgacgccgg aggcgtcctc cgtatcccag ggcagctcgg tgatgccgaa ataggtgctg
     4501 taataactga gcatgctgtc ggcgctgtac gccacccagt acagcagctc agcggcggtg
     4561 atgtccacat ctcccacctg ggccaccaca gtgtcacccg ggatgccggc ggtggtgagg
     4621 aacacatccg tgacggcgtc caggtccacg gtgatcggtt cactgctgga ggtgctcccg
     4681 gcggcactgg aagaagcgcc gctcccggag ctcccgccgt ccttaccgca ggcggtcagg
     4741 gaaaaggcca gaatcagggc cgcgagggtc agggcccccg cccgtttcat cgatctcata
     4801 agagcagttc ctttctgttt catccaattc agatctgttc cggtccgcag ggccggatac
     4861 agtttgatat cataccacat ctgtcttccg gtttcaatgg agaagggaaa aactccccac
     4921 tctgaaatgc ggggcggctt cagccctccg cccccgcgaa gtcgtccacc acctgccgtg
     4981 cggcccgcag ggcgtcctcc ccctttttca gccggaaggt aaagcggggc acctcccccg
     5041 gagtgagcag cagccggttc tgataccggt ccatggcgca cagggcggaa aactgctcca
     5101 gactgaaccg ggccagggta aagaccagct gcccctgctt ctgggcgatg tcggtgatcc
     5161 cggcctccgc cgccgcggcg cggagcaggg ccacggagat caggttgttc accgtgcggg
     5221 ggggctcgcc ataccggtcg atgagctcat ccaccaggtc gtccgcctcc gcctcgctgc
     5281 ggatggcggc gatgcggcgg tacaggtcca tccgctgctc cggggagggg acgtaccggt
     5341 cgggcaccga cgccgccacg ctcaggtcgg cggcacattc ggtcttggga ggcaccggct
     5401 ggccctgctc ctgaagcacc gcttcctcca gcagcttcaa gtacatgtcg tagcctaccg
     5461 acaacaggaa gccgctctgc tccggcccca gcacgttgcc cgcaccccgg atctccaggt
     5521 cccgcatggc gatcttgaag ccggagccga actcggcgaa ctcccggatg gcgctcagcc
     5581 gcttggcggc cacctcggtg agcaccttgc cccggcggta ggtgaggtag gcgtaggccc
     5641 ggcggctgga ccgccccacc cggccccgga tctggtggag ctgggccagg cccaggcggt
     5701 cggcgtcctc gatgatgagg gtgttggcgt tgggcaggtc gatgcccgtc tcgatgatgg
     5761 tggtgcacac cagcacgttc agctccccgt cggtcatccg gctcatcacc tcgtcgatgg
     5821 cctcctgggt catcttgccg tgggccaccc ccaccgccgc atcctccccc agcatggcct
     5881 ggatgcggct ggcggtgcgg tcgatggtct ccacccggtt gtggaggtag tacacctgcc
     5941 cgccccgctc cagctcccgg cgcatggcgt cccccagcac cccccagtcg tgctccagca
     6001 cataggtctg caccggctgg cggtcggcgg ggggctcctc cagggtggac atgtcccgga
     6061 tgccgctgag ggccatgttc agcgtccggg ggatgggggt ggcggacagg gtcagcacat
     6121 ccacctgacg gaagttctcc ttcagcttct ccttgtgctg gacgccgaag cgctgctcct
     6181 cgtccaccac cagcagaccc aggtccttga agcgcacgtc ccggttgaac aggcggtggg
     6241 tgccgatgag gatgtccacc tcaccgctct ccacccgccg cagggtctcc ttcatctggg
     6301 cgctggtgcg gaagcgggag accacatcga tggtcaccgg atatttggca aagcgccgca
     6361 gggcgttgag aaaatgctgc cgggcgagca ccgtggtggg caccagcaca gcggcctgct
     6421 tgccgtccag cacgcatttc atgacggccc ggaaggccac ctccgtcttt ccgtagccca
     6481 cgtcgccgca cagcagccgg tccatgggca ccgggcgctc catgtcccgc ttgatctcct
     6541 ctacgcagcg cagctggtcc tccgtctccg cgtactcgaa ctcctcctcg aattcccgct
     6601 gccaggggga gtcgggagag aaggcgaagc cgggctgccg ctgccgctgg gcatagagct
     6661 ggatcaggcc cttggccaga tccttcaccg cctttttggc cttgctcttg gccttctccc
     6721 agtcggtgcc ccccaggcgg gagagctttt tggtctccgc cgcgtcctcg cccccgccga
     6781 tgtacttgct caccaggtcc agctgggtgg cggggacgta gagcacatcg gagccggcgt
     6841 aggcgatctt cacatagtcc ttctccaccc cgtcggcagg cagtttcacc atccccatgt
     6901 agcggcccac accgtg
//
LOCUS       DBMDSJ010000038         6902 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3270_length_6902_cov_1.844876, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000038 DBMDSJ010000000
VERSION     DBMDSJ010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_6902_cov_1.844876"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="ACZPAQ_01715"
     CDS             complement(<1..215)
                     /locus_tag="ACZPAQ_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0902724.1"
                     /translation="MATILLVEDDTSMRLLTTAKLKHRYAIISVQDGVEALDVLERQP
                     VDLIIADVMMPRMDGYTLVKTLRERGD"
     gene            622..1935
                     /locus_tag="ACZPAQ_01720"
     CDS             622..1935
                     /locus_tag="ACZPAQ_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR0902725.1"
                     /translation="MRQRISLLLAVLILAGTLSLPTLAAEPGEDNARETGAQNGGVQQ
                     APADPEETQNPRPDPEETQNPRPDPEGTVTWANLDSRIREGSLSALILAENIGGIEGI
                     DYDLMYDDLRRQINDIADLQWMMVLMGNSMGADALDQTYDALRETFDAIKDGELQADN
                     ADVVWQLEDTVNQVVSGGETLYITLLGLEQQAADGQRGLAALDRSLEELRLRQTLGQV
                     SQQTVAQLEQTRVETVSQLNSLDATIRELKVQLQNLIGEAPTGELELGALPTRSETVW
                     EVPDYEAGLEAARAASWTLRSAQKALEDAEEDWKDARSDYRSSRKQYLLQQAEHTWNA
                     AQLTYQSTVQNFETSFKSLYDSLANYEQLYASAQSALVWQQSQLDTVQTRYDLGLTTC
                     SAVLDVQDEVASAQSALDSAWRDLFSACNSYRWAVEYGLLPAQGA"
     gene            2007..3227
                     /locus_tag="ACZPAQ_01725"
     CDS             2007..3227
                     /locus_tag="ACZPAQ_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR0902726.1"
                     /translation="MDCKLSVLLCGALTLALAGCQAPVEEPLLDPTVTVETTAVQAGD
                     LSTQSTYIGTISAEGTASVVSMISGNVEQVAVAVGDTVSAGDLLCRFDDESARLSLQN
                     AQAAANSAQESYNSAVSNYGGTDLPLLQEQLRLAQDNYDDTLALLEIGAASQVEVDQA
                     HQTLLSAQAGLEAAQASLNSARAGIQSAQVGVDSAQYQLSLYNLTAPINGVVEAVNVT
                     QNNFAASGQVAFVISNGNNKTVTFYVTDSVRQTLTPGQAVTVTYNGQSYEGAVTEIGG
                     VVDAQVGQFRVKAIIDGAQDLPDGLAVELTTTAHQVTGAVLIPSDAMFFESGVSYVYV
                     VQDGKAVRSDVTIGLYTADTIAVTSGLTLGDEVITTWSSSLRDGVSVQTAAQAEDSES
                     AQADPAASGPEAEG"
     gene            3234..6290
                     /locus_tag="ACZPAQ_01730"
     CDS             3234..6290
                     /locus_tag="ACZPAQ_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR0902727.1"
                     /translation="MKFIQLVLRRPMSIITLILGVVVFGSASLTGMPLEYMPDMEMPM
                     ELVMITWPGADADSVDRLLTQPMEDECESLSDLDSINSYSSENYTMLQLTYQYGTDMD
                     DAYSDLKSAIDNLMPSLPDECGDPIIMEISADALGTMMISATAPAGIDVADYLDNEVV
                     PALENIGGVARVELSGARDEYLRIVLDEAAMRQYGLSISTVGSAIAAADFDMPVGSVS
                     LGSQDIALGVYGNVEVNPNFRDLPIQTPSGRTVMLEDICTFFNLYEEDADTVSRYNGQ
                     ESVMLTVTKQDSAATMEVCNAVQDVLDQYSVDGVGFETIYSEGDSILETLGEVLNTLI
                     TGVILTMIVLFVFFGDLRASLIVGISMPLSILLAVILLNFAGFNIDLMTGSALIIAIG
                     MIVDNSIVVLESCMRCKEEGMDFREAAATGTATMLMSILAGTLTTVVVYIPMAMADGL
                     VGMMTGPLSWTILLTLLCSFLCAVVVVPLAFLWLKPRTKDQLITNRILGRFKGFYRRT
                     LPRLLRHPGRVVLVGGACFLAAILLMTQMEFVMMASNYDGSITVDVAFRSGTKVEVMN
                     QRIQTLEDALLSDENFESVTLDLSGNTASFTAYSVDNCDRSSEAAVEEYTARFGSVPD
                     MDVSVSPSGAMDMSALMSSNSKDVVLLGSDLDTLQTAAEQVEEAMTQVPGVIRIENPF
                     RSSQSKGRIVINSQKAMALGTSESAVAMQIYYLLEGMNATSVDYGDTEYDVVLEYPEG
                     KYDDISALLDYPITTQTGQQVALRDISTVEYITTLPTITRQEGQYSVTLTATTTDSAK
                     YSAPKEIDARTAQLDLPQGVSFGVGMMDESTAEGLESMATAIAAGIFLVFLVMAIQFD
                     SPRLSIMVMMCIPLSLIGSIGLVFLNGRPMSIVGLMGFLMLVGIAVNNGIYLVDGTNQ
                     LRQTMPLGDALVEAGTTRLRPILMTTLTTIISMVPMIFSNDSGMSMMKDMAYVMVGGL
                     IASTLLAMFLMPAFYLLIRRENLDGTKKGRRKKQQPEPVEAGNTQS"
BASE COUNT         1373 a         2158 c         2106 g         1265 t
ORIGIN      
        1 tggtcccccc gctcccgcag ggtctttacc agcgtatagc cgtccattcg aggcatcatc
       61 acgtcagcaa tgattaagtc cactggctgc cgttccagca cgtccagggc ctccacccca
      121 tcctggacgc tgatgatggc ataccggtgc ttcagcttgg cggtggtgag caggcgcata
      181 gaggtatcgt cttccaccag cagaatggtc gccattgccg tctcccttcc ccggccttgg
      241 tttgggaaga cccctctggg cagttctccc gcgccggagt tatttttgat agttccacaa
      301 caaaaatcaa cgaaagaaca acagcctagt atctcatcca tgggatatag ggtgtcacag
      361 acattatatc gtcccagggg ctgtccgtca aagggagagc ccgcctctgg aagaccagca
      421 tagccccttc gggtaaacta gacgtaaact tcctgtcgct ctttgtgggt ttttctaaaa
      481 aatgtggggc agagaggggg atttccggcg ttttcttcct gccgcccggg cggcccagtt
      541 tacaatgagt ttacctgagc gtgctatgct accgccgtta gaaaatctgc acgaacgcgc
      601 agcgacgatg gaggcacaac catgagacaa agaatttctc tgctgctggc tgtattgatt
      661 ctagccggta cgctgagcct tcccaccctg gccgcagagc cgggggagga caacgcacgg
      721 gagaccgggg cccagaatgg gggagtccag caggcccctg ctgacccgga ggagacccag
      781 aatccccggc ccgacccgga ggagacccag aatccccggc ccgacccgga ggggacagtg
      841 acctgggcca acctggacag ccgcatccgg gaggggagcc tgagcgccct gatcctggcg
      901 gagaacatcg gcggcatcga gggcatcgac tatgacctga tgtatgatga tttgcgccga
      961 cagatcaacg acatcgccga tctgcagtgg atgatggtcc tgatgggcaa cagcatgggt
     1021 gctgacgccc tggaccagac ctatgacgct ctgcgggaga cctttgacgc gatcaaggac
     1081 ggagagctgc aggcggacaa cgccgatgtg gtgtggcagc tggaggacac ggtgaaccag
     1141 gtggtctccg ggggcgagac gctgtacatc acccttctgg gcctggagca gcaggccgcc
     1201 gacggacagc ggggcctggc cgccctggac cggagcttgg aggagctgcg cctgcggcag
     1261 acgctgggcc aggtgtccca gcagacggtg gctcagctgg agcagacccg ggtggagacc
     1321 gtcagccagc tgaacagcct ggacgccacc atccgggagc tgaaggtcca gctccagaat
     1381 ctcatcggtg aggcccccac cggagagctg gaactgggcg ctctgcctac ccggtcggag
     1441 acagtgtggg aggtccccga ctatgaggcc ggcctggagg ccgccagggc ggccagctgg
     1501 accctgcgca gtgcccagaa ggcgctggag gatgcggaag aggattggaa ggacgcccgc
     1561 tccgactacc gctcctcccg caagcagtac ctgctccagc aggcggagca tacctggaac
     1621 gccgctcagc tcacctacca gtccacagtc cagaacttcg agaccagctt caaaagcctg
     1681 tatgactccc tggccaacta tgagcagctg tatgccagtg cacagtccgc tctggtttgg
     1741 cagcagtccc agctggacac cgtccagacc cgctatgacc tggggctgac cacctgctcc
     1801 gcggtgctgg atgtccagga cgaagtggca tctgcccaat ctgccctgga cagcgcctgg
     1861 agggacctgt tctccgcctg caacagctat cgctgggcgg tggagtacgg cctgctcccg
     1921 gcccagggag cataaacaga gacactcagg gcggcccctg ccggctgccc gcagtttgaa
     1981 gggaagaaag gatggaacac gactccatgg attgcaaact ttccgtttta ttgtgcggtg
     2041 ccctgaccct cgccttggct ggctgccagg cgccggtgga ggagcccctg ctggacccca
     2101 ccgtaacggt ggagaccacc gccgtccagg ccggggacct gagcacccag agcacataca
     2161 tcggcaccat ctccgcagag ggtactgcca gcgtggtatc catgatctcc ggcaatgtgg
     2221 agcaagtggc cgttgccgta ggggacaccg tctctgccgg ggacctgctg tgccgctttg
     2281 atgacgagag cgcccgcctg agcctgcaaa atgcccaggc cgcggccaac agtgcccagg
     2341 agagctataa cagcgcggtg tccaactacg gcggcacgga cctgcccctg ctgcaggagc
     2401 agctgcgcct ggcccaggac aactatgacg acactctggc cctgctggag atcggcgctg
     2461 cctcccaggt ggaagtggac caggcccacc agaccctcct gtctgcccag gctggcctgg
     2521 aggcggccca ggcatccctg aactctgccc gggcgggcat ccagtccgcc caggtggggg
     2581 tggattccgc ccagtaccag ctctccctct acaacctcac cgcccccatc aacggcgtgg
     2641 tggaggcggt gaacgtcacc cagaacaact tcgccgcttc cggtcaggtg gccttcgtca
     2701 tctccaatgg gaacaacaaa acggtcacct tctatgtcac cgactctgtg cgccagaccc
     2761 ttactcctgg ccaggcggtc accgtcacct ataacggcca gtcctatgag ggggcggtca
     2821 ccgagatcgg cggcgtggtg gacgcccaag tgggccagtt cagggtaaag gctattatcg
     2881 acggagccca ggacctgccc gacgggctgg cggtggagct gactaccacc gcccaccagg
     2941 tcaccggggc agtgctcatc cccagcgacg ctatgttttt tgaaagcggc gtctcctatg
     3001 tgtatgtggt ccaggacggc aaagcggtgc gctccgacgt gaccatcggc ctgtacaccg
     3061 ctgacaccat cgccgtaacc agcggcttga ccctggggga cgaggtcatc accacttggt
     3121 cctccagcct gcgggacggg gtctccgtgc agaccgccgc ccaggcggag gacagcgaga
     3181 gcgcccaggc cgatccggcg gcatccggcc cagaggcgga gggataacgc ggcatgaagt
     3241 tcattcaatt agtcctgcgg cgccccatgt ctatcatcac cctcatcctg ggcgtggtgg
     3301 tgttcggctc cgcttccctc accgggatgc ccctggagta tatgccggac atggagatgc
     3361 ccatggagct ggtgatgatc acctggccgg gggcagatgc ggacagcgta gaccggctgc
     3421 tgacccagcc aatggaggac gagtgtgagt ccctgtccga tctggattcc atcaactcct
     3481 actcctcgga aaactacacc atgctccagc tgacctatca atacggcacg gacatggacg
     3541 acgcctactc tgatctgaag agcgccatcg ataacctgat gccctccctc cctgacgagt
     3601 gcggcgaccc catcatcatg gagatcagcg ccgacgccct gggaaccatg atgatctccg
     3661 ccaccgcccc cgcgggcatc gacgtggcgg actatctgga caacgaggtg gtccccgccc
     3721 tggagaatat cggcggcgtg gcccgggtag agctgtccgg cgcccgggat gagtacctgc
     3781 gcatcgtgct ggacgaggcg gccatgcggc agtatggact gtccatttcc accgtgggct
     3841 ctgccatcgc ggcggccgac ttcgacatgc cggtgggcag cgtgtccctg ggctcccagg
     3901 acatcgccct gggcgtgtac ggcaacgtgg aggtcaaccc caacttccgg gatctgccca
     3961 tccagacccc ttccggccgc acggtgatgc tggaggatat ctgtaccttc ttcaacctgt
     4021 atgaggagga tgcggacacc gtcagccgct acaacggcca ggagagcgtc atgctcactg
     4081 tcaccaagca ggattccgcc gccactatgg aggtgtgcaa tgcggtccag gacgtgctgg
     4141 accagtacag cgtggacggt gtgggctttg agaccattta cagcgagggc gacagtatcc
     4201 tggagaccct gggcgaggtg ctcaataccc tcatcaccgg cgtcatccta accatgatcg
     4261 tgctgttcgt cttcttcggc gacctgcggg ccagcctgat cgtgggcatc agcatgcctc
     4321 tgtccatcct gctggcagtg atcctgctga actttgccgg cttcaacatc gacctgatga
     4381 ccggctcggc cctcatcatc gccatcggca tgatcgtgga taactccatt gtggtgctgg
     4441 agagctgcat gcgctgcaag gaagagggaa tggatttccg ggaggctgcc gccaccggca
     4501 cggccaccat gctcatgtcc atcctggcgg gcaccctcac caccgtggtg gtgtacatcc
     4561 ccatggccat ggccgacggc ctggtgggca tgatgaccgg ccccctgagc tggaccatcc
     4621 tgctgaccct gctgtgctcc ttcctgtgcg ctgtggtggt ggtcccactg gccttcctct
     4681 ggctcaagcc ccggaccaag gatcagctta ttaccaaccg catcctgggc cggttcaagg
     4741 gcttctaccg ccggaccctg ccccggctgc tgcgccaccc cgggcgggtg gtgctggtgg
     4801 gcggcgcctg cttcctggcc gccatcctgc tgatgaccca gatggaattt gtgatgatgg
     4861 ccagcaacta tgacggcagc atcactgtgg acgtagcttt ccgctcgggc accaaggtgg
     4921 aggtgatgaa ccagcgcatt cagaccctgg aggacgccct cctctccgac gagaacttcg
     4981 agagcgtgac cctggacctg tccgggaata ccgcctcctt caccgcctac tccgtagata
     5041 actgtgaccg ctccagcgag gcggcggtgg aggagtacac cgcccgcttc ggcagcgtgc
     5101 cggatatgga tgtgtctgtc tcccccagcg gcgccatgga catgagcgcc ctgatgagca
     5161 gcaacagcaa ggacgtggtc ctgctgggca gcgacctgga tacccttcag accgcagcgg
     5221 agcaggtgga ggaggccatg acacaggtcc ccggcgttat ccgcatcgag aaccccttcc
     5281 gctccagcca gtccaagggc cgcatcgtca tcaattccca gaaggccatg gccctgggca
     5341 cctctgagtc agcggtggcc atgcagatct actatttgct ggagggcatg aatgccacat
     5401 cggtggacta cggcgacacg gagtatgatg tggtcctgga gtaccccgag ggcaagtacg
     5461 acgatatctc cgccctgctg gactacccca tcaccaccca gactggccag caggtggccc
     5521 tgcgggacat ctccaccgta gagtacatca ctactctgcc caccatcacc cggcaggagg
     5581 ggcagtacag cgtcaccctt acggccacca ccaccgattc cgccaagtac agcgccccaa
     5641 aagagatcga cgcccggacg gcccagctcg acctgcccca gggggtgtcc ttcggcgtgg
     5701 gcatgatgga tgagagcacc gcagagggcc tggagagcat ggcgaccgcc atcgccgcgg
     5761 gcatcttcct ggtgttcctg gtcatggcca tccagttcga ctccccccgg ctgtccatca
     5821 tggtgatgat gtgtattccg ctgagcctta ttggctccat cggcctggtg ttcctcaacg
     5881 ggcggcccat gagcatcgtg ggcctgatgg gcttcctgat gctggtgggc atcgcggtga
     5941 acaacggcat ctatctggtg gacggcacca accagctgcg gcagaccatg cctctagggg
     6001 atgcactggt ggaggcgggc accacccgcc tgcgcccgat cctgatgacc accctgacca
     6061 ccatcatctc catggtgcct atgatttttt ccaatgacag cggcatgagc atgatgaagg
     6121 acatggccta cgtcatggtg ggcggcctta tcgcctccac cctcctggcc atgttcctga
     6181 tgcccgcctt ctatctgctg atccggcggg agaacctgga cggcaccaaa aagggccgcc
     6241 gcaaaaagca gcagcccgaa cctgtggagg ccggaaacac ccagtcctga ccgcccccaa
     6301 tagagacaag acaggagcgc cccggcgggg cgctcctgtc ttgtctttcc cactgtggac
     6361 agcttgcctt tttccgaggg ggagagctcc gggggagatc catgtgtgat ggaagcctgt
     6421 accatgtttt actatgaacg ggaaaggtat atgccgtcgg cagtggggct taaatatgta
     6481 ggcgcgtcca gatcccaaag gaacaggatc tcatagatcc ggctgacctc tataaatatt
     6541 cccttccacc tatcaaaagg attatgcaca atacaggacg cagctgcatc acactcagat
     6601 gaagacacaa ctgtttgcgg cacagtttgg ttggaaacgc ggtggagagg gcagacgtgg
     6661 gaagagagat atgagggagg aactgtgatg cccagagcac aggagaatcg tatttcgggg
     6721 gagtgacttt cttggctcct ggcgggggca gcttttgatc gactgcgggg atgttacact
     6781 gcgcaggaaa agaagacctg aaaaaatgac cctttcaaaa agaagaggag atgttggcat
     6841 taaaaatgct gcggagcata gctccgcagc tggggatatg agaggcaaac tgttttaaaa
     6901 ga
//
LOCUS       DBMDSJ010000039         6851 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3301_length_6851_cov_1.839704, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000039 DBMDSJ010000000
VERSION     DBMDSJ010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6851
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_6851_cov_1.839704"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            22..1368
                     /locus_tag="ACZPAQ_01735"
     CDS             22..1368
                     /locus_tag="ACZPAQ_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR0902728.1"
                     /translation="MAKLVYDDTGRLLFTKEMKEEYTILMPQMLPVHFGMFQRLLELE
                     GYKVDMLTSNHRGIIDEGLKYVHNDTCYPALLVIGQLIDAIKHGGYDPHKVALFITQT
                     GGGCRASNYIHLLRKALEKADLAFVPVISVNLSGLEKNPGFSLTLPIIRKMVYAMMYG
                     DIIVNVANQVRPYERERGATDAMVSTWQGRLIQGFQQGKGMSRGQMRTNFDAICADFA
                     SIPVTGEQKVRVGVVGEIYVKFSPLGNNNLEDFLLSEGAEPVVPGLTDFMIFKIYNRV
                     VDVDLYGGKWIKKAACQLFMKYIEACQKDMIQALERSGRFRAPGTFQDLHRMIQGYLG
                     DGNKMGEGWLLTAEMLELIHTGTPNIVCTQPFGCLPNHIVGKGMLRKLKDDYPYSNVV
                     AIDYDPGATKINQENRIKLMLANAKALAKQQGGEAPAAAREAAPTPSSYTHAGAHA"
     gene            complement(1475..2545)
                     /locus_tag="ACZPAQ_01740"
     CDS             complement(1475..2545)
                     /locus_tag="ACZPAQ_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024598.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR0902729.1"
                     /translation="MRGKRFVYGSYRGRRSTTDVLKTIALILAVLVVLAVAGVWMAQK
                     YIVYDDGGYHLELPFFQKKGPAQEPEDLRVAVEPAASQPEETPEEGEPLLAAVTVPLS
                     AVEDGSAEALAAQAGANSVLLDMKTDRGQLGFISQLAMAASAGVNAGQADINRQLQAL
                     NSGELYTVARLSCFRDHALAKDETYAIRTNSGYRWTDPEELRWSSPASQAVQDYLVAL
                     MVELAQLGFDEILLDNWGYPAEGNLGYIKQGDAYDLEHLSQPVEEFLARAEAALEPYG
                     TRLAFRSTWAVLEGDESPGGQTAQLLERYAQRVWLEAPEDGMADLLSQAGLPEGEERL
                     ALLVEELDPDLDCSQGIWDPEA"
     gene            complement(2665..3402)
                     /locus_tag="ACZPAQ_01745"
     CDS             complement(2665..3402)
                     /locus_tag="ACZPAQ_01745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR0902730.1"
                     /translation="MDRAVLITGASRGIGAAVARRFAREGWRVGANYRTSRGRAEALA
                     AEIAAAGGQTALLPGDVSGPAQAAALVRAAEEQLGPLDALVCNAGVALPQQLLTDTTD
                     EQWRQVMGVDLDGVFYTLRAAVPGFVRRQRGAIVTVSSMWGVTGGSCEVAYSAAKAGV
                     IGLTKALAKELGPSHITVNCVAPGVIDTEMNGGLDSEALAALAEETPLGRIGTAEEVA
                     ESVWFLAGGGAPFLTGQVIQPNGGIVI"
     gene            complement(3415..4572)
                     /locus_tag="ACZPAQ_01750"
     CDS             complement(3415..4572)
                     /locus_tag="ACZPAQ_01750"
                     /EC_number="4.2.1.51"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HIR0902731.1"
                     /translation="MEDCLAALRQEVDRLDDQIAELFLKRMEVTGRIGACKEELGLPL
                     MDPRRELEVLRSAAAETLDEGRKLELAHLFETVLALSRRDLRRRVGGNDPGPGYSRCR
                     RAEAAARQPVERPRVVYQGVAGAYSEMASLRFFGEDCRCQGLPTFEEVFQTVAQGGAD
                     YGVVPIENSSTGAIRQVYELLGRYECYIVGENTVKVEHCLMAPHGATLDTITHVYSHE
                     QGLFQCEPFLARHPGWVGVAHGDTAGSAQYVAQTGDITKAAICSSRAAELYGLEILAR
                     GVNYNSNNTTRFVVVSPVMELREGADKISAVLSLPDEVGSLHEVLTIFAVHGLNLVKL
                     ESRPLPGRSWEYLFFLEFDGSLTDPAMDGALQELSQASAEMRVLGNFKSNL"
     gene            complement(4585..4920)
                     /locus_tag="ACZPAQ_01755"
     CDS             complement(4585..4920)
                     /locus_tag="ACZPAQ_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HIR0902732.1"
                     /translation="MNGETTRDLLLILVMAVVTFATRLLPFLLFDRGEEPPKAVLYLG
                     RVLPPAIMAMLIVYCLKGVSFATPAGWVPALLSCGAVVLLHLWKGNDLLSIFGGTILY
                     MALVQGVFG"
     gene            complement(4917..5645)
                     /locus_tag="ACZPAQ_01760"
     CDS             complement(4917..5645)
                     /locus_tag="ACZPAQ_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR0902733.1"
                     /translation="MDRNVLSAAFHVTIPVLMGYLAIGMAFGLMMDSAGYNVFWALLM
                     SLIIYAGAGQYMGVTLLAAGASPLQTALMTLLLNFRHMFYGLSMLEKFRGMGLRKLYM
                     IFSLTDETYALLASAKAPEGVREKDYYLAIALMDHSYWVLGTVLGSLLGSALSFDTTG
                     VDFAMTALFIVIAVGQWKGTRNHLPAAIGCLATLASLRLAGEDNMLILALVVIVAALL
                     VFSPKMPEGQALHKAGTGKEERTL"
     gene            5974..>6851
                     /locus_tag="ACZPAQ_01765"
     CDS             5974..>6851
                     /locus_tag="ACZPAQ_01765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR0902734.1"
                     /translation="MLFLYNPTAGKGRVTADLAAVLDVFTKAGYLTTVYPTQGKADAT
                     RIAAELGGQFDRVVCSGGDGTLSETVSGLMQLDHPPVLGYIPFGSTNDCATTLRLPRD
                     PVKAAQTASETGVPRPWDIGRLNGRPFVYVAAFGAFTEVSYDTPQDLKNTFGHLAYVM
                     AGIASLPSITPYHLRIECDDQVLEDDFFYGMVCNTISVGGMKALPTDRVVLDDGLFEV
                     ILVRKPTSITDLTDSLQALIRQEPPQSGAVLSFHASHLKFTAEKPIPWTLDGEYGGSH
                     EINVVENCRQALTVMQG"
BASE COUNT         1238 a         2413 c         1996 g         1204 t
ORIGIN      
        1 tcggcagaaa gggattttga tatggcgaag ctagtatatg acgacaccgg ccgtctcctc
       61 tttaccaagg agatgaagga ggagtacacc atcctcatgc cccagatgct gcccgtccac
      121 ttcgggatgt tccagcgtct gctggagctg gagggctaca aggtggatat gctcacctcc
      181 aaccaccggg gcatcatcga tgaagggctc aaatatgtcc acaacgacac ctgctacccc
      241 gccctgctgg tcatcggaca gctgatcgac gccatcaagc acggcggcta tgacccccac
      301 aaggtggccc tgttcatcac ccagaccggg ggcggctgcc gggcctccaa ctacatccac
      361 ctgctgcgca aggcgctgga aaaagcggac ctggcctttg tgccggtgat ctcggtgaat
      421 ctgtccggtc tggagaaaaa tcccggcttc tccctcactc tccccatcat ccgcaagatg
      481 gtctacgcca tgatgtacgg ggacatcatc gtcaatgtgg ccaaccaggt ccggccctat
      541 gagcgggagc ggggcgccac tgacgccatg gtctccacct ggcagggccg gctgatccag
      601 ggcttccagc agggtaaggg gatgagccgc ggccagatgc ggaccaactt tgacgccatc
      661 tgcgccgact ttgcctccat ccccgtcacc ggggagcaga aggtgcgggt gggcgtcgtg
      721 ggtgagatct atgtaaagtt ctcccccctg ggcaacaata atttggagga ctttctgctc
      781 tccgaggggg ccgaacccgt ggtccccggc ctcaccgatt tcatgatctt caagatctac
      841 aaccgggtgg tggacgtgga tctgtacggc ggcaagtgga tcaagaaggc tgcctgccag
      901 ctctttatga agtacatcga ggcctgtcag aaggacatga tccaggcgct ggagcgctcc
      961 ggccgcttcc gtgcaccggg caccttccag gacctgcacc ggatgatcca gggctatctg
     1021 ggggacggca ataagatggg agagggctgg ctcctcaccg ccgagatgct ggagctcatc
     1081 cacaccggca cccccaacat cgtctgcacc cagcccttcg gctgtctgcc caaccacatc
     1141 gtgggcaagg gcatgctgcg caagctgaag gacgactatc cgtacagcaa tgtggtggct
     1201 atcgactacg accccggcgc caccaagatc aaccaggaga accgcatcaa gctgatgctg
     1261 gccaacgcca aggcgctggc caagcagcag ggcggggaag cccccgccgc cgcccgggag
     1321 gcggccccca ccccctcctc ctacacccat gccggggccc acgcgtaaat tctatcttcc
     1381 agagggcggg gctacatggc tccgccctct ttgggagaaa aacaaggcgt ctgagctctc
     1441 actcagacgc cttgcctgtt tcctcgtacg gagttcacgc ctccgggtcc cagatcccct
     1501 gggaacagtc caggtctggg tccagctctt ccaccagcag ggccagccgc tcctctcctt
     1561 ctgggagccc cgcttgggaa agcaggtccg ccatgccgtc ctccggcgcc tccagccaga
     1621 cccgctgggc ataccgctcc agcagctggg ccgtctggcc gccgggactc tcatccccct
     1681 ccagcaccgc ccaggtgctc cgaaaggcca gccgcgtccc gtagggctcc agagccgcct
     1741 ccgctctggc gaggaactcc tccaccggct gagacaggtg ctccaggtcg taggcatccc
     1801 cctgcttgat atagcccaga tttccctccg ccggatagcc ccagttgtcc agcaggatct
     1861 cgtcaaagcc cagctgggcc agctccacca tcagagcgac cagatagtcc tggaccgcct
     1921 gactggctgg gctgctccac cgcagctcct ccggatcggt ccaccggtag ccgctgttgg
     1981 tccggatggc atatgtctca tccttggcca gggcgtggtc ccggaagcag gacaggcggg
     2041 ccaccgtgta cagctccccg ctgttgaggg cctggagctg gcggttgatg tccgcctgtc
     2101 cggcgttgac ccccgccgag gcggccatgg ccagctggga gatgaagccc agctggcccc
     2161 ggtctgtctt catgtccagc aggacgctgt tggctcctgc ctgggcggcc agcgcctccg
     2221 ccgagccgtc ctccaccgct gaaaggggaa cagtcaccgc cgccagaagg ggctctccct
     2281 cctccggtgt ctcctccggc tgggaggcgg cgggctctac cgccacccgg aggtcctccg
     2341 gctcctgggc cggtcccttc ttttgaaaga agggcagctc caggtgataa cccccgtcgt
     2401 cgtagacgat gtatttctgc gccatccaca ctccggccac ggccagcacc accagtaccg
     2461 ccaggatcag ggctatggtc ttcaacacgt ctgtggtgct cctccgtccc cggtaggagc
     2521 cgtacacgaa ccgcttcccg cgcatcacgg acctcctttc ccacccaccc ggcgtgaaag
     2581 gggtccgccc tctccctcgg ggggtcgtct catttcagta tgccgccttt cggcggcgct
     2641 attccctctc tcccgctccc gcggtcagat cacgatcccg ccgttgggct ggatcacctg
     2701 tccggtgagg aagggggcgc cgccccctgc caggaaccac acgctctccg ccacctcttc
     2761 cgccgtgccg atccggccca ggggggtctc ctccgccaga gcggccaggg cctcgctatc
     2821 cagtcccccg ttcatctccg tgtcgatgac cccgggggcc acgcagttga cggtgatgtg
     2881 ggagggcccc agctccttgg ccagcgcctt ggtcaggccg atgacccccg ccttcgcggc
     2941 ggaataggcc acctcgcagg agccgccagt gaccccccac atggaggata cggtgacgat
     3001 ggcccctcgc tgccggcgga caaagccggg gaccgccgcc cgcagggtgt agaacacccc
     3061 gtccagatcc acccccatca cctggcgcca ctgctcgtcc gtggtgtcgg taagcagctg
     3121 ctggggcagg gcgacccctg cgttgcacac cagggcgtcc agggggccca gctgttcctc
     3181 cgccgcccgg accagagcgg ccgcctgggc cgggccagac acatcgccgg gcaggagggc
     3241 ggtctgtccc ccggcggcgg cgatctctgc tgccagggcc tccgcccggc cacgggaggt
     3301 gcgataattc gcccccacac gccagccctc ccgggcaaag cggcgggcca ccgccgcccc
     3361 gatgccccgg gaagctccgg tgataagcac agccctgtcc atcgcctctc ctccttacag
     3421 gttgcttttg aagtttccca ggacccgcat ctccgcggat gcctgggaca gctcctggag
     3481 ggcaccgtcc atcgccgggt ccgtcagact cccgtcaaac tccaggaaga acagatactc
     3541 ccagctccgt cccggcagag ggcgggactc cagcttcacc aggttcagcc cgtggacggc
     3601 aaagatggtg agtacctcat ggaggctgcc cacctcgtcc ggaaggctga ggaccgcgga
     3661 gatcttgtcc gccccctccc gcagctccat caccggggag accaccacga agcgggtggt
     3721 gttgttgctg ttgtagttga ctccccgggc cagaatctcc aggccataca gctccgccgc
     3781 ccggctggaa cagatggccg ccttggtgat atccccggtc tgggccacat actgagcgct
     3841 gcccgccgtg tccccgtggg ccacccccac ccagccggga tgccgggcca ggaagggctc
     3901 gcactggaag agcccctgct cgtgggaata gacatgggtg atggtatcca gggtagcccc
     3961 atggggagcc atcaggcagt gctctacctt gacggtattc tcccccacga tgtagcactc
     4021 atacctgccc agcagctcgt acacctggcg gatagccccg gtggagctgt tttcgatggg
     4081 caccacgccg tagtccgccc cgccctgggc cactgtctgg aacacctctt caaaggtggg
     4141 caggccctgg caccggcagt cctctccaaa aaaacgcagg gaggccatct cgctgtatgc
     4201 ccccgccacg ccctgataaa ccaccctggg tctctccacc ggctgccgcg cggcggcctc
     4261 ggccctccgg catcggctgt atcccggacc gggatcgttc cctcccaccc ggcgccgcag
     4321 gtcccggcgg ctgagggcca gcacagtctc aaacaggtgg gccagctcta gtttccggcc
     4381 ctcatccaac gtctccgccg ctgcgctccg gagcacctcc agctcccgcc gggggtccat
     4441 caggggcagc cccagctcct ccttgcaggc cccgatccgg ccggtcacct ccatgcgctt
     4501 caaaaacagc tccgcgatct gatcgtccag gcggtccacc tcttgccgca gggcggccaa
     4561 acagtcttcc atttccgctc ctccttaccc aaacacgccc tggaccaggg ccatatacag
     4621 gatcgtccct ccgaagatgg agagcagatc gttccccttc cacagatgga ggaggaccac
     4681 cgccccgcag gagaggaggg cgggtaccca ccccgccggg gtggcaaagc tgacgccctt
     4741 caggcagtag acgatcagca tggccatgat ggccgggggc agcacccgcc ccagatagag
     4801 caccgccttg gggggctcct ccccccggtc aaagagcagg aagggcagca gccgggtggc
     4861 aaaggtcacc acggccatca ccaggatcag gagcagatcc cgtgtggtct ctccgttcac
     4921 agcgtccgct cctcctttcc tgtcccggcc ttgtgcaggg cctgcccctc cggcatttta
     4981 ggggaaaata ccagcagggc ggccacgatg accaccagcg ccaggatcag catattatcc
     5041 tcccccgcca gccgcaggct ggccagggtg gccaggcagc cgatagcagc tggcagatgg
     5101 ttccgggtcc ccttccattg gcccaccgcg atgacgatga agagggcggt catggcgaag
     5161 tctactccgg tggtgtcaaa ggacagggca ctgcccagca gggagcccag aaccgtgccc
     5221 agcacccaat aactgtggtc catcagggca atggccaggt agtagtcctt ctcccgcacc
     5281 ccctccggcg ccttggcgga cgccagcagc gcataagtct catcagtgag ggaaaagatc
     5341 atatagagct tccgcagccc catcccccgg aacttttcca gcatagacag gccgtagaac
     5401 atgtgccgga agttcagcaa cagggtcatc agagccgtct gaagggggga ggcccccgca
     5461 gccagcagag tcacccccat gtactgtccg gccccggcat agatgatcag gctcatcagc
     5521 agggcccaga acacattgta tcccgccgaa tccatcatca ggccgaaggc catcccgatg
     5581 gccagatagc ccatcagcac cggaatggtg acatggaaag cggcggagag gacgtttcga
     5641 tccatctctt tcattcaccc catagcggtg gtgccgtctg ctgagacggg gcagcacaca
     5701 cctccgccgg gcaccaagtt tgttgcgttc cacattttac tcgtttttgg ccggatacgc
     5761 aagggatttt cccacttttt ccgccatttt ccgggaacgg gcccgttcct ttcttttctc
     5821 ctgtacagat gacataaaga ttcacaaatt ttcccttgtt ttttccgtcc cattccttat
     5881 aataattcgt aatcagcagc atccggggag tccaaggacg gggcgctccg ggcgccaatc
     5941 tcataaaaaa gtgaggcccc agctttgaaa accatgcttt ttctctacaa ccccaccgcc
     6001 ggcaaggggc gggtgacggc cgatctggcc gctgttctgg atgtctttac caaggcgggc
     6061 tacctgacca ccgtctatcc cacccagggg aaggcggatg ccacccgcat cgccgccgag
     6121 ctgggcggcc agttcgaccg ggtggtctgc tccggcgggg acggcacgct cagcgagacg
     6181 gtgtccggcc tgatgcagct ggaccatccg ccggtgctgg gttacatccc ctttggctcc
     6241 accaacgact gcgccaccac cctgcgcctg ccccgggacc cggtgaaagc cgcccagacc
     6301 gcctcagaga ccggcgttcc ccgcccctgg gatatcggca ggctcaacgg ccgccccttc
     6361 gtctatgtgg ccgctttcgg cgccttcacc gaggtatcct acgacacccc ccaggatctg
     6421 aaaaacacct tcggccatct ggcctatgtg atggcgggca tcgcctccct cccctccatc
     6481 accccctacc acctgaggat cgagtgcgac gaccaggtgc tggaggacga tttcttttac
     6541 ggaatggtgt gcaacaccat ctccgtaggc ggaatgaagg ccctgcctac agaccgggtg
     6601 gtgctggatg acgggctgtt tgaggtcatt ctggtccgca agcccaccag catcaccgac
     6661 ctcactgaca gcctccaggc ccttatccga caggagcccc cccagtcagg ggccgtgctc
     6721 tccttccacg cctcccatct gaagttcacc gccgaaaagc ccattccctg gacgctggat
     6781 ggggagtacg ggggcagcca cgagatcaac gtagtggaga actgccgcca ggccctgact
     6841 gtgatgcagg g
//
LOCUS       DBMDSJ010000040         6848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3302_length_6848_cov_1.674818, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000040 DBMDSJ010000000
VERSION     DBMDSJ010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3302_length_6848_cov_1.674818"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="ACZPAQ_01770"
     CDS             complement(<1..731)
                     /locus_tag="ACZPAQ_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902735.1"
                     /translation="MSWERMTAIGPAGENRWSRWRRLLVPSLFFAGVIYYEELFLKLF
                     CFHAVTPVGALFTLLFTLPIAILLGLLCGGVAPRKGRVLLPAMTLLLSVWMGAQSIYY
                     HLFKTFLTAFSLTKMGMVAGAFGDMAVGEILLNWFPIAMMAVPFGLAVVFRRRLIPEE
                     GPMSRRMGVRWALLAAVVQLAAMGIVMCCGGGSMSLRFIYLQAAVPELEVQNFGMLTQ
                     NQLELRRVLFGITPEDQSLRKGQAL"
     gene            1221..1760
                     /locus_tag="ACZPAQ_01775"
     CDS             1221..1760
                     /locus_tag="ACZPAQ_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902736.1"
                     /translation="MSELRDTLLARLSEAYTAYFDVEPVQDGSPLKALCAYHSRGSQY
                     VLIKKAELWAAETHEYLYLYSLPRLDFTSLVELQARTLEDGMPRIRPHSQHMCTYLSA
                     VVLCDQADPDALAQLQKLKKHRDFKLSLHGWMEFRIAAVDLSTGDIATNRSGKDLGKS
                     LSGLVAREIQKFKLRRGET"
     gene            1761..3506
                     /locus_tag="ACZPAQ_01780"
     CDS             1761..3506
                     /locus_tag="ACZPAQ_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR0902737.1"
                     /translation="MNAVLVLSIGIVILIVGYIFYGGWLAKQWGVDDSRPTPAHELED
                     GMDYVPAKAPVLMGHHFSSIAGAGPINGPIQAAVFGWMPVVLWVLIGGIFFGAVHDYG
                     ALFASIRHKGQSIGEVVALNIGDRAKKLFLIFSYLTLVLVVAAFASIVASTFQATYVD
                     GVLDTAASATNASVAMISLLFIVMAVLFGFFVYRRGASLAVATIAGCVGIVICLLIGL
                     NWHPIYLSNSVWMWIIGAYILVASVAPVWILLQPRDYLSSFLLYAMMAIAAVGVIGAG
                     LTGADAAHMDMPAFTGFYDNLAPTGTSLGYVFPALFVTIACGAISGFHSLVGSGTTAK
                     QLDHERDAKPIAYGGMLIECALALVSLCAVSFIWTEYASGEIVTPTQVFATGISRMIA
                     SIPGLAGTQATVSSLLVLTVSVFCLTSLDTATRLARYMFQEFWLKPGQTYKEATGFKA
                     ILTNPVVATAITVVLGVGLGMTGYSKIWPLFGAANQLLAALALLAVCAWLGNIGKNNK
                     MFYIPMIFMLIVTLTSLAQTISTQVGKITAGAATDWAPYAQAILGVLLFILAIVLAVE
                     GCMTIFGKKKNTAAK"
     assembly_gap    3546..3557
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3681..4337
                     /locus_tag="ACZPAQ_01785"
     CDS             3681..4337
                     /locus_tag="ACZPAQ_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019559.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HIR0902738.1"
                     /translation="MPAFLLLEGVLYLSFLALDLLSPGPLSTALKYAALLLCLLMALA
                     GASRGGDRLVAAALAMTAAADLFLLVLDRWYLLGILLFYGVQVAYMVRICKANGGYAL
                     LIPRFLLFSLLLVALYQLELFTPETAAAALYFSHFLFNALLSLSLRDAGGRLFSLGLL
                     LYLMCDMLVAVSQFPSLFPAPLSSVAQVGMWLFYLPGQVLIVLSGLVIPSGGVPDETE
                     "
     gene            4324..4980
                     /locus_tag="ACZPAQ_01790"
     CDS             4324..4980
                     /locus_tag="ACZPAQ_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIR0902739.1"
                     /translation="MKQSKILSVLLAVLTACLVLSSSVAVPILCRPFYYAHIGPLGLV
                     QATGLTEEAIRQAYDEMLDFCLGAPEFSTGALSWSQSGRDHFADVRVLFLLDLAVLAL
                     SAALLLLWFLLGRRMGLGPRPLLGRGPGFWAAAGLGIVFAVVGLLASLDFERAFVVFH
                     ALFFPGKDNWLFDPAVDQIILILPEVFFRNCALLIGGLILLWCAILIGTDLWLGRRRR
                     "
     gene            complement(5046..5924)
                     /locus_tag="ACZPAQ_01795"
     CDS             complement(5046..5924)
                     /locus_tag="ACZPAQ_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR0902740.1"
                     /translation="MRYDSDLLYRPPGEWRSYLLQCTIGCSHNKCTFCAMYKEKKFRV
                     RPVEEILEDIDMARSYYGPNVERVFLMDGDAIVMRTEQLLQILEKLYAAFPKLQKVTT
                     YAGPRSTLSKTPEELRALREAGLTRAYLGVESGSDAVLQAIQKGCTAAEMLQAGQNLV
                     AAGIDLWAIVILGLTGQGGDWEEHVLSTAGIINKMKPRHLSAMTFAPAAGTPLGEDVL
                     AGRFQVCTPDQILEECHLLLEHLDVDPLHFTSNHASNYLPLKGGLPEDREKFLSLIDQ
                     ALEGKIRLRKTLNRGI"
     gene            complement(5928..>6848)
                     /locus_tag="ACZPAQ_01800"
     CDS             complement(5928..>6848)
                     /locus_tag="ACZPAQ_01800"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0902741.1"
                     /translation="MDTDRLRKYPDPASSGLIKELADYYGLKEDQVFVGVGSDDVIAM
                     AFLTFFNSKKPVLFPDITYSFYQVWAELFGIPYEMPALDKEFRLRPEDYKRENGGIIF
                     PNPNAPTGLYMPLDQVEEILRANQESVVIVDEAYIDFAGASALELTDRYENLLVVQTF
                     SKSRSMAGMRIGYALGSQDLIAGVNCVKNSFNSYPLDRLAQAAGEAAVRDTAYFEETR
                     GKIMATRTWVTHRLREMGFQVHDSSSNFLFIAHPDHSGRVLQQGLRDRGVLVRRFERP
                     RIQDHLRVSIGTDEEMEAMCRAMEEILKGQ"
BASE COUNT         1124 a         2291 c         1905 g         1516 t
ORIGIN      
        1 tggagcgcct gccccttccg gaggctctgg tcctccgggg tgatcccgaa gagcactctg
       61 cgcagctcca gctggttctg ggtcagcatc ccaaagttct gcacctccag ttccggcacc
      121 gccgcctgga gatagatgaa gcgcagggac atggagcccc cgccgcagca catcacgatg
      181 cccatggcag ccagctggac caccgccgcc agcagggccc accgtacccc catccttctg
      241 gacatggggc cctcctccgg gatcagacgc cggcggaaga ccaccgccag tccgaagggg
      301 accgccatca tggcgatggg gaaccagttg agcaggatct ccccaaccgc catgtctccg
      361 aaagcaccgg ccaccatccc catcttagtc agggagaaag ctgtgaggaa ggtcttgaac
      421 aggtgataat agatgctctg ggctcccatc cacacggaga gcagcagggt cattgccggc
      481 agcagcaccc gcccctttcg tggggcgacc ccgccgcaca gcagtcccag caggatcgcg
      541 atgggcaggg tgaacaacag ggtgaacagc gcccccaccg gcgtgacggc gtggaaacaa
      601 aacagcttca aaaacagttc ttcatagtag atgaccccag caaaaaacag ggacgggacc
      661 agcagccgcc gccaacggct ccatctgttc tcccccgccg ggcctatagc agtcattctt
      721 tcccaactca tgtagacaac ctccgctcgc gggactcact tttgggaaga gcgccctgtc
      781 cgtctccgcg cctccccctc tctccggggg ccggcgcggc ctggcgcatg atttcccatt
      841 gtactcaatc ccgcacccgc tgtcaaatcc ccggaacgtc tcccctctcc ccacgccctc
      901 catatttccc ctataaaaaa cggcggtaat cctgcacagc gctgtccctt tttttctttt
      961 ttctcccatc ccgcggtttt ctgcacaatt tcttctcctt cttctttatt tttttgttta
     1021 ttctatctta atcaaaagaa tttgatggag gatcttcatt tctttcctcc ctttttgaag
     1081 aatttccttg caaaatatgt ggaaatctgt tatcatgatt ttaacatttt attcataatt
     1141 ttggggttta tttgggcggg agcgggaatc ggtttccgct tctgttctgc ctatggcgcc
     1201 ccatggaaat gaggtcccct gtgtctgaac tgcgcgatac cctcctcgcc cgcctctcgg
     1261 aggcatacac agcctatttt gacgtagagc ccgtccaaga cggttccccc ctgaaagcac
     1321 tgtgcgccta tcactcccgc ggctctcagt acgtactgat caaaaaggcg gagctgtggg
     1381 cggcggagac ccacgaatac ctctacctat actccctccc ccggctggac ttcaccagcc
     1441 tggtggagct tcaggcccgc acgctggagg acgggatgcc acgcatccgc ccccattccc
     1501 agcatatgtg cacctatctg tcggccgtgg tcctgtgtga tcaggccgac ccggacgctc
     1561 tggcccagct tcaaaagctg aagaagcacc gggactttaa gctatcgctc catggatgga
     1621 tggagtttcg tatagcagcg gtggacctgt ccaccggcga catcgccacg aaccgcagcg
     1681 gcaaagatct ggggaagagc ctgagtggtc ttgtggcccg cgagatccag aaatttaagt
     1741 taaggagagg agaaacttaa atgaatgcag tgttagttct ttccattggc attgtcattc
     1801 tgatcgtagg ttacatcttc tacggcggat ggcttgccaa gcagtggggc gtggatgact
     1861 cccggcccac ccctgcccat gagctggagg acggcatgga ctatgtcccc gccaaggctc
     1921 cggtgctgat gggccaccac ttttcgtcta tcgccggcgc cggccccatc aacggcccca
     1981 tccaggccgc cgtattcggc tggatgcctg tggtgctgtg ggtgctgatc ggcggcatct
     2041 ttttcggcgc cgtccatgac tacggtgccc tgttcgcttc catccgccac aagggccagt
     2101 ccatcggcga agtggtcgcc ctgaacatcg gcgaccgtgc caagaagctg ttcctgatct
     2161 tttcctacct gaccctggtg ctggtggtgg ccgctttcgc ctccatcgtg gccagcacct
     2221 tccaggccac ctatgtggac ggcgtgctgg acaccgccgc ctctgccact aacgcgtccg
     2281 tggccatgat ctccctgctg ttcattgtca tggccgtcct gttcggcttc tttgtctacc
     2341 gccgcggggc ctccctggca gtggccacca ttgccggctg cgtgggcatc gtgatctgcc
     2401 tgctcatcgg cctgaactgg caccccatct atctgtccaa ctccgtgtgg atgtggatca
     2461 tcggcgccta cattctggtg gcctctgtgg ctcccgtgtg gatcctgctc cagccccgtg
     2521 attatctgtc ctccttcctg ctgtacgcca tgatggccat cgccgctgtg ggcgtcatcg
     2581 gggccggtct cactggtgcg gacgctgccc acatggatat gcccgccttc accggcttct
     2641 atgacaacct ggcgcccacc ggcacctccc tgggctatgt cttccccgcc ctgttcgtca
     2701 ccatcgcctg cggcgccatc tccggcttcc actccctggt gggctccggc accaccgcca
     2761 agcagctgga ccacgagcgg gacgccaagc ccatcgccta cggcggcatg ctcatcgagt
     2821 gtgcgctggc cctggtctcc ctgtgtgctg tgtccttcat ctggaccgag tacgcctccg
     2881 gcgagatcgt cacccccacc caggtgtttg ccaccggcat ttcccgcatg attgcctcca
     2941 tccccggtct ggcgggcaca caggccaccg tcagctccct gttggtgctg accgtatctg
     3001 tcttctgcct gacctccctg gatactgcca cccgtctggc ccgctacatg ttccaggagt
     3061 tctggctgaa gcccggccag acctataagg aggccaccgg cttcaaggcc atcctcacca
     3121 accccgtggt cgccaccgcc atcaccgtgg tgctgggcgt gggtctgggc atgaccggct
     3181 actccaagat ctggcccctg ttcggcgccg ccaaccagct gctggccgct ctggccctgc
     3241 tggccgtctg tgcctggctg ggcaacatcg gcaagaacaa caagatgttc tacatcccca
     3301 tgatcttcat gctgatcgtc accctgactt ccctggctca gaccatcagc actcaggtgg
     3361 gcaagatcac cgccggcgct gccaccgact gggcccccta tgcgcaggcc atcctgggcg
     3421 tgctgctgtt catcctggct atcgttctgg ctgttgaggg ctgcatgacc atcttcggca
     3481 agaagaaaaa caccgccgcg aagtaacctt ccatcctgct tcacgacatc agcaaggacc
     3541 gcagtnnnnn nnnnnnnggg cgctgcggtc ctcttctgtt gacaaggatt gtattttttt
     3601 ctcattcgtg ctacactgaa cgggacagcc tttgtggaag ggaggagaac attctgatcg
     3661 agcggctctg ccggtggagc gtgccggctt ttttgctgct ggagggggtg ctctacctct
     3721 ccttcctggc cctggacctg ctctctcctg gtcctctgag caccgccctg aagtatgccg
     3781 ccctgctgct gtgcctgctc atggccctgg cgggggcttc ccggggcgga gaccgcctgg
     3841 tggccgccgc gctggccatg accgcagcgg ccgacctctt cctgctggtg ctggaccgct
     3901 ggtacctcct gggcatcctc ctcttttacg gggtccaggt ggcgtatatg gtccgcatct
     3961 gtaaggccaa cggaggctat gccctgctca ttcccaggtt ccttctgttc tccctgcttc
     4021 tggtggccct gtaccagctg gagctcttca caccggagac cgcagccgcg gccctctact
     4081 tctcccactt cctcttcaac gccctgctga gcctgtccct gcgggacgcc gggggccgcc
     4141 tcttctccct gggactgctc ctctacctca tgtgcgacat gctggtcgca gtctctcagt
     4201 tccccagcct cttccctgcc cccctctctt ccgtggccca ggtagggatg tggctcttct
     4261 acctccccgg tcaggtgctc atcgtcctgt ccggtctggt catcccttcg ggaggtgtcc
     4321 ctgatgaaac agagtaagat cctgtccgtc ctgctggccg tccttacggc ctgcctcgtg
     4381 ctctcctcct ctgtggcggt ccccatcctc tgccgccctt tctattacgc ccatatcggc
     4441 cctttggggc tggtccaggc taccggcctg acagaggagg cgatccgcca ggcctatgac
     4501 gagatgctgg acttctgcct gggcgccccg gagttctcca ccggcgcgct gtcctggtca
     4561 cagtccggcc gggaccattt tgccgatgtg cgggtcctct tcctgctgga tctggccgtg
     4621 ctggccttgt ctgccgccct cctgctcctc tggttcctgc tgggccggcg gatgggcctg
     4681 gggccccgcc cccttctggg ccggggcccc ggcttctggg ctgccgcggg gctgggcatc
     4741 gtcttcgccg tggtgggcct gctggcctcc ctggactttg agcgggcttt tgtggtattc
     4801 cacgccctgt tcttcccggg gaaggacaac tggctcttcg acccggcggt ggaccagatc
     4861 atcctgattc tgccggaggt ctttttccgc aactgcgctc tgctcatcgg ggggctgatc
     4921 ctgctctggt gcgccatcct tatcgggacg gacctatggc tgggacgccg ccgccgctga
     4981 accgataaag accgccccgc cggaaccaaa ggctccggcg gggcggtctt cactttcggg
     5041 acatctcaga tcccccgatt cagggtcttg cgcaggcgga tcttgccttc cagcgcctgg
     5101 tcgatcaggg agaggaactt ctcccggtcc tccggcagcc cccccttcag gggcaggtag
     5161 ttggaggcgt ggttgctggt gaagtggagg gggtccacat ccaggtgctc cagcagaagg
     5221 tggcactcct ccaggatctg gtccggggta cacacctgaa agcgcccggc cagcacgtcc
     5281 tcccccagcg gggtgcccgc cgcaggggca aaagtcatgg ctgagaggtg gcggggcttc
     5341 attttgttga tgatgccggc ggtggacagg acgtgctcct cccagtcgcc cccctggccg
     5401 gtgaggccca ggatgacgat ggcccacagg tcgatgcccg ccgccaccag gttctggccc
     5461 gcctggagca tctccgcggc ggtgcagccc ttttggatgg cctggagcac tgcgtcgctg
     5521 ccgctctcca cccccagata ggcccgggtc agtccggcct cccgcagggc gcgcagttcc
     5581 tccggcgtct tgctcagggt gctcctgggg ccggcgtagg tggtcacctt ctggagctta
     5641 gggaaggcag catacagctt ctccaggatc tggagcagct gctccgtcct catcacaatg
     5701 gcatccccgt ccatgaggaa gacccgctcc acattgggcc cgtagtagct gcgggccatg
     5761 tcgatgtcct ccaggatctc ctccaccggg cggacccgaa acttcttctc cttgtacatg
     5821 gcacagaagg tgcatttgtt gtgggaacac ccgatggtgc actggagcag atagctgcgc
     5881 cactcccctg ggggacggta cagcaggtca ctgtcgtatc gcatgggtca ctgtcctttc
     5941 aaaatctctt ccatagcccg gcacatggcc tccatctcct cgtctgtccc gatggacacc
     6001 cgcagatggt cctgtatcct ggggcgttca aaccggcgca ccagcacgcc ccggtcccgc
     6061 agcccctgct ggagcacccg cccggagtgg tccgggtggg cgatgaacag gaaattggag
     6121 ctggagtcat ggacctggaa gcccatctcc cgcagcctgt gggtgaccca cgtccgggtc
     6181 gccatgatct tgccccgggt ctcctcaaaa tatgccgtgt cccgcacggc cgcctcccct
     6241 gccgcctggg ccagccggtc cagggggtag gagttgaagg agttcttcac gcagtttacc
     6301 ccagcgatca ggtcctggct gcccagggcg tagccgatcc gcatccccgc catagaccgg
     6361 gatttgctga aggtctgcac cactaaaagg ttctcatacc ggtctgtcag ctccagtgca
     6421 gaagcccctg caaaatcaat atacgcctca tccacaatga ctaccgactc ctggttggcg
     6481 cgaaggatct cctccacctg atccagaggc atataaagtc ccgttggagc attgggatta
     6541 gggaaaatga tccctccgtt ttctctttta taatcctccg gacgcaggcg gaattctttg
     6601 tccagagccg gcatctcata ggggataccg aaaagctctg cccacacctg atagaaggaa
     6661 taggtaatat ccgggaacag aaccggcttt ttggagttaa aaaaggtgag gaaggccatg
     6721 gcaatcacgt cgtccgagcc tactcccaca aacacctgat cctccttcag cccataataa
     6781 tctgccagtt cttttataag gccgctgctg gctggatccg ggtactttct cagacggtct
     6841 gtgtccat
//
LOCUS       DBMDSJ010000041         6840 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3308_length_6840_cov_1.847158, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000041 DBMDSJ010000000
VERSION     DBMDSJ010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3308_length_6840_cov_1.847158"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="ACZPAQ_01805"
     CDS             complement(<1..863)
                     /locus_tag="ACZPAQ_01805"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR0902742.1"
                     /translation="MKNSEKTMEKIVALCKGRGFIFSGSEIYGGLANTWDYGPLGVEL
                     KNNVKKAWWKKFVQENPYNVGLDAAILMNPQVWVASGHVGGFSDPLMDCKECKERFRA
                     DKVIEDWCQENSFELGHSVDAMSQAEMKAFVEEHNICCPTCGKFNWTDIRQFNLMFKT
                     FQGVTEDAKNTVYLRPETAQGIFVNFQNVQRTTRRKLPFGVCQIGKSFRNEITPGNFT
                     FRTREFEQMELEFFCKPGTELEWFAYWKQFCHDWLLGLGIRDENLRLRDHDPEELSHY
                     SNATTDFEFVFP"
     misc_binding    complement(944..1150)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1322..2080
                     /locus_tag="ACZPAQ_01810"
     CDS             1322..2080
                     /locus_tag="ACZPAQ_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0902743.1"
                     /translation="MDHIRFGAGGRVLVILPGLGDGLRTVRETALPLAAACREYGRER
                     KVYLFSRRRGLEPGATIRGMAEDQAEAMKKLGLWGADVLGISQGGMVAQHLAAEAPEL
                     VRRLVLVATAGWANETVQETVGRWLLLAERGDHRRLMRDTAERSYSQRYLRRYRLLLP
                     LLGTFGRPRSYQRFLIQARACLGHDARGALGRIGCPALVVGGGRDQVVGAEAAGVLAE
                     HLSQSRLWIYPELGHGLYEEAADLPARVLAFLSQ"
     gene            2233..2490
                     /locus_tag="ACZPAQ_01815"
     CDS             2233..2490
                     /locus_tag="ACZPAQ_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902744.1"
                     /translation="MMQTLYYTTHNFVRRSGNVIDLCEYRSRMQDVQEEPVSAAPAAR
                     TERTRRRHPHQNAFALLMDSFASMSVILMTLTFTFWVLDKL"
     gene            2586..2963
                     /locus_tag="ACZPAQ_01820"
     CDS             2586..2963
                     /locus_tag="ACZPAQ_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR0902745.1"
                     /translation="MKKAIHTDRAPAAIGPYSQAVRAGGFLFTSGQIPVDPAAGDIPA
                     GIKAQAQQSLDNIAAILAQAGLDRSAVVKTTVFLKDMNDFAAMNEVYAQFFEGPAYPA
                     RSAVEVARLPKDVLVEIEAVALL"
     gene            3247..4344
                     /gene="ychF"
                     /locus_tag="ACZPAQ_01825"
     CDS             3247..4344
                     /gene="ychF"
                     /locus_tag="ACZPAQ_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR0902746.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITNAGAESANYPFCTIDPNVGMVAV
                     PDHRLDKLSEMYHPKKTTPAVIEFVDIAGLVKGASKGEGLGNKFLSNIRMTDAIVHVV
                     RCFDDENVIHVEGSTDPIRDMDTIDLELVMSDMEMVDRRIDKAQKAAKGDKKFLHEAE
                     VFQGLREWLDQGKSARGYLPQVPEDDAALIATSELLSLKPVIYAANLDEDGFSDPEAV
                     PYYRLVEERAAAQGAQVIPVCAKLEAEIAELDAEEKKMFLDDLGVAESGLDRLVKASY
                     TLLGLISYLTSGEDECRAWTIRRGTKAPQAAGKIHSDFERGFIRAEVVSYDDLMACGS
                     MAAAREKGLIRSEGKEYIVQDGDIILFRFNV"
     gene            4439..4885
                     /locus_tag="ACZPAQ_01830"
     CDS             4439..4885
                     /locus_tag="ACZPAQ_01830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0902747.1"
                     /translation="MKLLEINARSQELLTCLCQVWESSVRATHHFLSEEDIRSLAVYV
                     PQALAQVPVLVVAFSDQGIPVGFMGIDGTRLEMLFLSPDVRGTGLGRQLLQRGIRRYG
                     VTSLCVNEQNPQALDFYLHMGFQVRSRTETDEQGNPFPLLCMSLPG"
     gene            4960..5445
                     /locus_tag="ACZPAQ_01835"
     CDS             4960..5445
                     /locus_tag="ACZPAQ_01835"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR0902748.1"
                     /translation="MINTTLCYIRQGGQCLMLHRVKKENDLNQGKWIGIGGKFEDGES
                     PEDCMFREVNEETGLTPAEYRYRGLVTFVSDRWPTEYMHLFTIDRFTGTLRADCPEGD
                     LEWLPWTQLCTIPHWEGDEIFLRLIEEDVPFFSLKLRYRGDILEEAVLNGRHVKGGPQ
                     L"
     gene            5442..6320
                     /locus_tag="ACZPAQ_01840"
     CDS             5442..6320
                     /locus_tag="ACZPAQ_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR0902749.1"
                     /translation="MTYTLSNDLLTVSIQDAGAQLCSVRSAGGTEYLWQADPAVWGRH
                     APLLFPLIGRLQDSQYTLDGRAWSISTHGFARDAQFQVSEQGPTTLSFQLEDSEETRR
                     VYPFSFVLTVTYTLEGSRLTKAHRVENRSAVPMLYELGGHDGFRTPLEPGETMADYAV
                     TISGVEELRPYGMDSRCMLTIGEARFPLEGGRIPLCPRAFGLDTIVLDLEGERRAALV
                     DRSGRERVVVECPDFPYLGLWTADKPFDTGYFCIEPWSALPDAVFVGRGLEDKAGIRR
                     LEPGGVHVLTYTTTFQ"
     gene            6340..6585
                     /locus_tag="ACZPAQ_01845"
     CDS             6340..6585
                     /locus_tag="ACZPAQ_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902750.1"
                     /translation="MKPEYANTFGLRKVADPQGQILEVTLDISYKYMENALTVTSNGL
                     ENVSTPNADHVASIVMNRQSAISLRNLLIQSLGDETL"
BASE COUNT         1279 a         2101 c         2174 g         1286 t
ORIGIN      
        1 gggaagacga actcaaagtc ggtggtggcg ttggagtagt ggctcagctc ctcggggtcg
       61 tggtcccgca gacgcaggtt ctcgtcccgg atgcccaggc ccagcagcca gtcgtggcag
      121 aactgcttcc agtaggcgaa ccactccagc tcggtccccg gcttgcagaa gaactccagt
      181 tccatctgct caaactcacg ggtgcggaag gtgaagttgc ccggggtgat ctcgttgcgg
      241 aaggacttgc cgatctgaca cacgccgaag ggcagcttcc ggcgggtggt gcgctggacg
      301 ttctggaagt tgacaaaaat gccctgggcg gtctcggggc ggaggtagac ggtgttcttg
      361 gcgtcctcgg tgaccccctg gaaggtcttg aacatcaggt tgaactggcg gatgtccgtc
      421 cagttgaact tgccgcaggt ggggcagcag atgttgtgct cctccacaaa ggccttcatc
      481 tccgcctggc tcatggcgtc cacgctgtgg cccagctcga agctgttctc ctgacaccag
      541 tcctcgatga ccttgtcggc gcggaagcgc tccttgcact ccttacagtc catcaggggg
      601 tcagagaagc cgcccacatg gccggaggcc acccacacct ggggattcat caggatggcg
      661 gcgtccagac ccacgttata ggggttctcc tggacaaact tcttccacca tgccttcttc
      721 acattgtttt tcagttccac acccagagga ccgtagtccc aggtgttggc caggccgcca
      781 tagatctcag agccggagaa gataaatccc cgtcccttgc acagagcgac gatcttttcc
      841 atggtctttt cactgttttt catgtgtcaa gctccttttt gtcgtgattt tagttgtaca
      901 cggtcattcc ggcggggcaa aaaaacgccc tgagccggtg tcggctcagg gcgaacaaaa
      961 acttgtccgt ggttccacct gaattcgtgc ctggctggca cgcactctga cccgataacg
     1021 gcgggggacc ggcgggccat ttcctgcccg cggctccagg gggccttcac ccgcgccatc
     1081 ggaggactcg cactgtccgt cctctcgctg caccccggcg ggggttactc ttccctttca
     1141 aagccatgcg gctgttttta gatacagcat cattctacta taaaaaaaac acggcgtcaa
     1201 gggccgacgg gccttttccc cgcttgtgag gcggcaggga aagtggtatc atggaaaaaa
     1261 tgaaggaggg gtagcggtgc ttggcgtgga gcgcggaacc gtgcgggggg agggctggga
     1321 gatggatcac atccgatttg gagcgggggg acgggtcctg gtcatcctcc ctggactggg
     1381 ggacggcctg aggaccgtcc gggagacggc cctgcccctg gcggcagcct gccgggagta
     1441 cggcagggag cggaaggtct atctgttcag caggaggcgg gggctggagc ccggggccac
     1501 catccgcggg atggcggagg accaggcgga ggccatgaaa aagctgggac tctggggtgc
     1561 ggatgtgctc ggcatctccc agggcggaat ggtggcgcag cacctggcgg cggaggcacc
     1621 ggagttggtg aggcgcctcg tcctggtggc gacagccggc tgggccaacg agacggtgca
     1681 ggagaccgtg gggcgctggt tgctgctggc ggaacgcggg gatcaccgaa ggctgatgcg
     1741 ggacacagcg gagcgctcct actcccagcg gtatttgcgg cgataccggc tgctgctgcc
     1801 cctgctgggc accttcgggc ggcccaggag ctaccagcgc ttcctcattc aggccagagc
     1861 ctgcctcggg catgacgccc gcggcgcgct gggacggatc ggctgtcctg cactggtggt
     1921 gggcggcggc cgggatcaag tggtgggggc agaggcggcc ggggtgctgg cagagcactt
     1981 gagccaaagc cggctgtgga tctatcccga gctgggacat ggactctacg aggaggccgc
     2041 agacctccca gcgcgggtac tggcattttt gtcccagtaa atcgaaaata tgttccattc
     2101 ctcttgacaa aatagaacat atgttgtatt ttaaaagtag aacatatgtt ttcttgcggg
     2161 gcagagaaaa agtccggttt ttgggaccgg cacactgtca tactgcagat aagattcagg
     2221 aacggaggga acatgatgca gaccctgtac tataccaccc ataatttcgt ccgccgctcc
     2281 ggcaatgtga tagatctgtg cgaataccgc agccggatgc aggacgtcca ggaggagccg
     2341 gtttccgccg ctcccgcagc ccggacagag cgcacacgcc gccgccatcc ccaccagaac
     2401 gcctttgccc tgctgatgga cagctttgcc agcatgagcg tgattctgat gaccctgact
     2461 ttcaccttct gggtgctgga caaactgtga ggttgcgccc gtgggggaaa cgtgctacaa
     2521 tgacggcagc cgccgggcac cgccgccccg gcgcaaatcg gatcgatcga gaggagcttg
     2581 attccatgaa gaaagccatt catactgacc gggcccccgc cgccatcggg ccctattccc
     2641 aggccgtccg ggccggaggg ttcctgttca cctctgggca gattccggtg gaccccgctg
     2701 ccggggatat cccggcgggc atcaaggccc aggcccaaca gtctctggat aacatcgccg
     2761 ccatcctggc ccaggccggg ctggaccggt ccgccgtggt gaagaccact gtgtttctca
     2821 aggacatgaa cgatttcgcc gccatgaacg aggtgtacgc ccagttcttt gagggacccg
     2881 cctatcctgc ccgctccgcg gtggaggtgg cccgtctgcc caaggatgta ttggtggaga
     2941 tcgaggccgt ggccctgctg taaggggacg gaatgcacag aaaaagcgct cgggaggccg
     3001 gcttgtccag ccccgggcgc tttgcgcgtc cagagacagg ggcctctgtg gccccgccgg
     3061 acacatctcc ccggcggcgg gccgcctgct ttgccgacgg ggacaagaca gactgccccg
     3121 cataatgggg gccccagccc gccgagggga aagacggcgg gagttgggcc ggaagccctt
     3181 gcaagacaga gaaaaatccg atataataac aggcactgaa ttttaagaga aaagagggct
     3241 tctgccgtga aattaggtat cgtgggtctg cccaacgtgg gcaagagcac cctgttcaac
     3301 gcgatcacca acgccggcgc cgagagtgcc aattacccct tctgcaccat cgaccccaat
     3361 gtgggcatgg tggctgtccc tgaccaccgg ctggacaagc tttccgagat gtaccacccc
     3421 aaaaagacca cccccgcagt cattgagttt gtggacatcg ccggtctggt aaagggcgcc
     3481 tccaagggcg agggactggg caacaagttc ctgtccaaca tccgtatgac cgatgccatc
     3541 gtccatgtgg tccgctgctt cgacgacgag aacgtcatcc acgtggaggg ctccaccgac
     3601 cccatccggg acatggacac cattgacctg gagctggtca tgtccgatat ggagatggtg
     3661 gacaggcgca ttgacaaggc acagaaggcc gccaaggggg acaagaagtt tctccatgag
     3721 gcggaggtgt tccagggcct gcgggagtgg ctcgaccagg gcaagtctgc ccggggctat
     3781 ctgccccagg tgccggagga tgatgccgcc ctcatcgcca ccagcgagct gctctccctc
     3841 aagccggtga tctacgccgc caatctggat gaggatggct tctccgaccc ggaggccgtc
     3901 ccctactacc ggctggtgga ggagcgggcc gccgcccagg gcgcccaggt catcccggtc
     3961 tgcgccaagc tggaggcgga gatcgccgag ctggatgcgg aggagaagaa gatgttcctg
     4021 gacgatctgg gggtggccga gtccggcctg gaccgcctgg tgaaggccag ctacaccctg
     4081 ctgggcctga tctcctacct caccagcggc gaggacgagt gccgggcctg gaccatccgc
     4141 agggggacca aggcccctca ggccgccggc aagatccact ccgacttcga acggggcttt
     4201 atccgggctg aggtggtctc ctacgacgac ctgatggcct gcggctccat ggccgccgcc
     4261 cgggagaagg gcctgatccg ctccgagggc aaggaataca tcgtccagga tggcgatatc
     4321 atcctgttcc ggttcaatgt atgaccggca tcccgctata ttctgatctg cacccattta
     4381 agctcggtcc gggccccgaa ggggcccgga ccgtttttcc cgaaaggagc cttttgcctt
     4441 gaaacttttg gaaatcaatg cccgctctca ggagttgctc acctgcctgt gccaggtgtg
     4501 ggagtcctcc gtccgggcca cccaccactt cctgtccgaa gaggacatcc gttccctggc
     4561 tgtctacgtc ccccaggctc tggcccaggt cccggtgctg gtggtagcct tctccgatca
     4621 gggcatcccc gtgggattta tgggcatcga cggcaccagg ctggagatgc tcttcctctc
     4681 cccagacgtg cgggggaccg gtctgggccg gcagctgctc cagcggggca tccggcggta
     4741 cggtgtcacc tctttgtgcg tcaatgagca gaaccctcaa gccctcgatt tctatctcca
     4801 catgggtttt caggtccgca gccgcacgga gacagacgag cagggcaatc ccttccctct
     4861 cctctgcatg tccctgccgg gataggcgcc tcccttgcgc ccttcttccc gccgtgctat
     4921 actgagggca gcactattat ggaaaggcgg tatcctgcca tgatcaacac caccctctgc
     4981 tacatccgtc agggcggtca gtgtctgatg ctccaccggg tcaagaagga gaatgatctg
     5041 aatcagggca agtggatcgg catcggcggc aagttcgagg acggggagag ccccgaggac
     5101 tgcatgttcc gggaagtgaa cgaggagacc ggactcaccc cagcggaata ccgctatcgg
     5161 gggctggtca cttttgtctc cgaccgctgg cccacggagt acatgcatct gttcaccatc
     5221 gaccgcttta ccggaaccct gagggccgac tgcccggagg gcgacctgga gtggctgccc
     5281 tggacacagc tgtgcaccat cccccactgg gagggggacg agatctttct gaggctcatt
     5341 gaggaggatg tccccttctt ctctctgaag ctgcgttacc ggggggacat actggaagag
     5401 gctgtcctca acggccggca tgtgaaggga gggccccagc tatgacctat acgctctcca
     5461 acgacctgct caccgtctcc atccaggacg ccggtgccca gctctgctcc gtccggagcg
     5521 ctgggggcac tgagtacctg tggcaggccg accccgccgt ctggggcagg catgccccgc
     5581 tgctattccc cctgatcggc cggctccagg acagccagta caccctggac ggccgggcct
     5641 ggtccatctc cacccatggg tttgcccggg acgctcagtt ccaggtgtca gagcagggcc
     5701 ccaccaccct ctccttccag ctggaggaca gcgaggagac ccggcgggtg tacccgtttt
     5761 cctttgtcct caccgtcacc tacacattgg agggcagccg cctgaccaag gcgcaccggg
     5821 tggagaaccg ctccgccgtt cccatgctct atgagctggg cggacacgac ggcttccgga
     5881 cccccctgga gcccggagag actatggcgg actacgccgt taccatctcc ggggtggagg
     5941 agctccgtcc ctacggcatg gacagcaggt gtatgctgac catcggcgag gcccgctttc
     6001 ccctggaggg agggcgcatc cccctgtgcc ccagggcctt cgggctggac accattgtcc
     6061 tggacctgga gggggagcgc cgggctgccc tggtggaccg ctccggccgg gagcgggtgg
     6121 tggtggagtg ccccgatttt ccctatctgg gactgtggac ggcagacaag ccctttgata
     6181 ccggctactt ctgtattgag ccctggtctg ccctccccga cgcggtcttt gtgggccggg
     6241 gcctggagga caaggcgggc atccgccggc tggagccggg gggcgtccat gtcctgacct
     6301 ataccaccac atttcagtga atcggaagga gaagatccca tgaaaccgga atacgccaac
     6361 accttcggcc tgcgcaaggt cgctgacccc cagggccaga tcctggaggt caccctggat
     6421 atctcctata agtatatgga gaatgccctc accgtcacct ccaacgggct ggagaacgtc
     6481 tctacgccca atgcggatca cgtggcctcc atcgttatga accggcagag tgccatttct
     6541 ctgcgcaacc tccttatcca gtccctgggg gacgagactc tctgagccgg gtggaaaggc
     6601 cggagccttt tgatgggaca acggccattc ggccaaaagg tccgcagctg cggaagaaaa
     6661 tattgaaaac aggtaaattg cggcggagag gacgtgtgtc ctctccgccg cccagcttgt
     6721 gggtccctct ttttcagaga aagatggttc aggatcaggc gggggagctc gagggggcgg
     6781 aagcccgcct cgagctcccc cgcctccctt gcacagcaag ggaggcggcg agcaaagcga
//
LOCUS       DBMDSJ010000042         6826 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3314_length_6826_cov_1.700818, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000042 DBMDSJ010000000
VERSION     DBMDSJ010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3314_length_6826_cov_1.700818"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="ACZPAQ_01850"
     CDS             <1..371
                     /locus_tag="ACZPAQ_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR0902751.1"
                     /translation="DGSDYETYSFGRSYLKGCHLGGDGFAVLLLGRYQAGGAEQVLTI
                     DPQCQLIAQAPLRGQVLSFSAAGRYLSLLTGSGLEIYTRDMSLYRALEDTQDARFTAL
                     SPNGSALLADSQQAWLYIPS"
     gene            407..1174
                     /locus_tag="ACZPAQ_01855"
     CDS             407..1174
                     /locus_tag="ACZPAQ_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014704.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR0902752.1"
                     /translation="MSFVIFDVVILILLLLTAWKGYRRGFVLTLCSLLAVFVAFIGAT
                     VISSQLARPMSRLIQPALERSITQVLEEQTHAVFPPPETSEASQPPALSDAFSGEEGQ
                     EGEEDWLAQLPLDEVLDALQASSLYQHFIQPIQDAIQDGVMEVTTSAARAVADYLALE
                     LTRMFLFLVSFALVLLLWLLLSRVLDLACKLPVLSTLNHWSGAALGLVKGGLIVFILC
                     WLLRGSLISQAAVEGTVLLRFFCTHTPLSLLAPLLFR"
     gene            1289..3598
                     /locus_tag="ACZPAQ_01860"
     CDS             1289..3598
                     /locus_tag="ACZPAQ_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR0902753.1"
                     /translation="MQPTMCSRCHKHVAVVFITKLEGGQSKNEGLCLKCAKELGIKPI
                     DDMIQKMGITDEDLENLTSEMMSAFGGAEGMEGLMPQDREDGDEDDGRTATFPFLNQL
                     FGAAAGGDAEPKKDAAPRPDKNGGKGQERPVKRKFLENYCISLTQRAQEGKLDRLIGR
                     ERELERVIQILNRRQKNNPCLIGEPGVGKTAIAEGLAMKIAKGDVPYKLLDKEVYLLD
                     LTALVAGTQFRGQFESRMKGLIEEIKKLGNIILVIDEVHNIVGAGDAEGSMNAANILK
                     PALSRGELQVIGATTLNEYRKHIEKDTALERRFQPVIVEEPSIEDSVQIIEGIAPYYE
                     AYHQVVISPEMCRLAVTMSERYITDRYLPDKAIDLIDEACSNVNLENRTLARRAEVDK
                     ELADLDKEKEVMMAAATEESYARLAAIRSQELRLEQEKGQLDAAPTPALTVEHLANVI
                     ELWTKIPASQIQEREYQRLARLEERLKQHIIGQDEAVHAVSSAIRRGRVGISPKRKPV
                     SFIFVGSTGVGKTELVKQLAHDLFNTPESLIRLDMSEFMEKHAVSRIIGSPPGYVGYD
                     EAGQLTEKIRRKPYSVILFDEIEKAHPDVLNILLQILDDGRITDAHGRTVNFENTVIV
                     MTSNAGSNTKSGSVGFARTANEQGRERAMKALQEFLRPEFINRVDEIVYFNQLTEDNF
                     KAIAALMLQELQDSLSEKGIVFTWQDSLLDYLVKKSYSAAYGARNLRRLIQKELEDAI
                     ASRIIDSWQHPVARLDASSDGEQLVLSAL"
     gene            complement(3751..4956)
                     /locus_tag="ACZPAQ_01865"
     CDS             complement(3751..4956)
                     /locus_tag="ACZPAQ_01865"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR0902754.1"
                     /translation="MNILVINAGSSSLKYQLLNPETQQVLAKGLCERIGIDGKFTYKP
                     QMEGKQVLDAIDIAMPTHSEAIQAVLDALVDKDNGVIGSMKEIDAVGHRVVHGGEAFN
                     QSVLITDEVMKALEDCIPLAPLHNPANITGINACTAVMGKDVPQVAVFDTAFHQTMPA
                     KAYMYALPYEYYEKDKVRRYGFHGTSHKYVAGRAAAMLGKPIEELKLISCHLGNGSSV
                     TAIDGGKSVDTTMGFTPLAGLPMGTRSGDLDAGILEYIMGKYGYDIKEMVSILNKKSG
                     VQGVSGVSSDFRDLENAHKEGNERAGLAVDMFNYGVKKYIGAYAAAMGGVDAIIFTAG
                     VGENSASQRLAIASDLEFMGVKMDAEANSVRGKETVISAADSKVKVLLIPTNEELMIA
                     MDTAAIAKG"
     gene            5141..6325
                     /locus_tag="ACZPAQ_01870"
     CDS             5141..6325
                     /locus_tag="ACZPAQ_01870"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetate ligase"
                     /protein_id="HIR0902755.1"
                     /translation="MKTAGIVAEYNPFHTGHAYQIARTRGLLGADCAVVAVMSGNWVQ
                     RGACAAADKWTRAGWALAGGADLVLELPTVWAASAAESFATGAVALLRACGVVDVLSF
                     GSESGDPAALAAAAACLDSEAYRAGLSRFLDEGMPFAACRQAVVAALLGSERAKVLSR
                     PNDNLGVEYLRAASALGWSPQVLAVPRQGAGHDAPRPAEGFASASILREWIAAGRRDL
                     ADCFLPAPWPEELEPTSLSHLERALLARVRSLSQAEWALLPDSGVEEGLPARLVQAGS
                     RALSVEEFLTLAKTKRYSHARLRRLLLWAFLGLTAADRPAAPPYLRVLGFTPRGQELL
                     RAMKGAASLPILTKPAHARSLDGPGRRLFQLEARCTDLYGLCFSTPRPGGLEWTTGPV
                     LI"
     gene            6344..>6826
                     /locus_tag="ACZPAQ_01875"
     CDS             6344..>6826
                     /locus_tag="ACZPAQ_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024136.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HIR0902756.1"
                     /translation="METKEKTTRRLRPLLPLLPPVLLSLLFYLFRGRQALMDGWVIHV
                     MTPAEQLLGRLSAPLPFSLAEALVTVFLTACALCLIRGTVLAVRLHAPHELGRRLVCL
                     LCALLWVWTGYCWLWSAGYYSASFAEKSGLETRPYTVSTLAQVTLYFACQAADLSTQM
                     V"
BASE COUNT         1120 a         2345 c         2088 g         1273 t
ORIGIN      
        1 ccgacggctc cgattatgag acctactcct ttggccgcag ctacctcaag ggctgccacc
       61 tgggcgggga cggcttcgca gtgctcctgc tgggccgcta ccaggccggc ggtgccgagc
      121 aggtgctcac catcgacccc cagtgccagc tcatcgccca ggcccccctc cgggggcagg
      181 tgctctcctt ctccgccgcc gggcgctacc tgtccctgct caccggcagc ggcctggaga
      241 tctacacccg ggacatgtcc ctctaccgcg ccctggagga cacccaggac gcccgcttca
      301 ccgccctctc ccccaacggc agcgccctgc tggccgacag ccagcaggcc tggctctata
      361 tcccctcctg agttcccttt tctcctgatc caagcgaggt attttgatgt cttttgtgat
      421 ttttgatgtt gtcatcctca tcctgctcct cctcaccgcc tggaagggct accgccgcgg
      481 ctttgtgctg acgctgtgct ccctgctggc cgtctttgtg gccttcatcg gtgccaccgt
      541 catctccagc cagctggccc ggcccatgag ccgcctgatc cagcccgctc tggagcggag
      601 catcactcag gtgctggagg aacagaccca tgcggtgttc cccccgcctg agacctccga
      661 ggcctctcag ccccccgccc tttcggacgc cttcagcggg gaagagggcc aggaaggcga
      721 ggaggactgg ctcgcccagc tccccctgga cgaggtcctg gacgcccttc aggcctccag
      781 cctgtaccag cactttatcc agcccatcca ggacgccatc caggatgggg tgatggaggt
      841 cactacctcc gccgcccggg cggtggcgga ttatctggcc ctggagctga cccggatgtt
      901 cctcttcctg gtctcctttg ccctggtcct gcttctctgg ctgctgctga gccgggtgct
      961 ggacctggcc tgcaagctgc ctgtcctgtc caccctgaac cactggagcg gcgccgccct
     1021 cgggctggtg aaggggggac tgatcgtgtt catcctctgc tggcttctga ggggctccct
     1081 gatctcccag gcggcggtgg aggggacggt gctcctccgc tttttctgca cccacacccc
     1141 tctctccctg ctggcgcccc tgctctttcg ttgagtttct ctctgtgtgc ccagcctttt
     1201 cttgcgaatt cacgacaagt tcacatttcg gggttatcct gtctttgttc caaaaaattt
     1261 ctctctcccg gaaggagtgt ttgcatagat gcagcccacc atgtgcagcc gctgccacaa
     1321 gcatgtggcg gtggtcttta tcaccaaatt ggaaggcggc cagtccaaga atgagggact
     1381 gtgcctcaag tgcgccaagg agctgggcat caagcccatc gacgacatga tccagaagat
     1441 gggcatcacc gacgaggacc tggaaaacct caccagtgag atgatgtccg ccttcggcgg
     1501 ggccgagggg atggagggac tgatgcccca ggaccgggag gacggggacg aggacgacgg
     1561 acgcaccgcc actttcccct tcctgaacca gcttttcggc gccgccgccg gcggggacgc
     1621 cgagcccaag aaggacgccg ccccccggcc ggacaagaac ggcggcaagg ggcaggagcg
     1681 ccccgtgaag cggaagtttc tggagaacta ctgcatctcc ctgacccagc gcgcccagga
     1741 gggcaagctg gaccggctca tcggccggga gcgggagctg gagcgggtca tccagatcct
     1801 caaccgccgc cagaagaaca acccctgcct catcggcgag cccggcgtgg gcaagaccgc
     1861 catcgccgag gggctggcca tgaagattgc caagggcgac gtgccctata agctgctgga
     1921 caaggaggtc tatctgctgg acctcaccgc cctggtggcg ggcacccagt tccgcggcca
     1981 gttcgagagc cggatgaagg gcctcattga ggagatcaaa aagctgggca atatcatcct
     2041 ggtcatcgac gaggtccaca acatcgtggg cgccggcgat gccgagggct ccatgaacgc
     2101 cgccaatatc ctcaagcccg ccctgagccg gggggagctc caggtcatcg gcgccaccac
     2161 cctcaatgag taccgcaagc acattgagaa ggacaccgcc ctggaacggc gcttccagcc
     2221 cgtcatcgtg gaggagccct ccatcgagga cagcgtgcag atcatcgagg gcattgcccc
     2281 ctactacgag gcctaccacc aggtcgtcat ctcccccgag atgtgccgcc tggccgtcac
     2341 catgagcgag cggtatatca ccgaccgcta tctgcccgac aaggccatcg acctcatcga
     2401 cgaggcctgc tccaatgtga acctggagaa ccgcactctg gcccgccggg ccgaggtgga
     2461 taaggagctg gccgacctgg acaaggagaa agaggtcatg atggccgccg ccaccgagga
     2521 gtcctatgcc cggcttgccg ccatccgcag ccaggagctt cggctggagc aggagaaggg
     2581 acagctggac gccgccccca cccccgccct gacggtggag catctggcca acgtcatcga
     2641 gctgtggacc aagatccccg ccagccagat ccaggaacgt gagtaccagc gcctggcccg
     2701 gctggaggag cggctgaagc agcacatcat cggtcaggat gaggcagtcc acgccgtctc
     2761 ctccgccatc cggcggggcc gtgtgggcat cagccccaag cgcaagccgg tgtccttcat
     2821 ctttgtgggc tccaccggcg tgggcaagac cgagctggtg aagcagctgg cccacgacct
     2881 gttcaacacg ccggagtccc tgatccggct ggacatgtcg gagttcatgg agaagcacgc
     2941 ggtctcccgc atcatcggct cccccccggg gtatgtgggc tatgacgagg cgggccagct
     3001 gacagagaag atccgccgca agccctactc cgtcattctc ttcgatgaga tcgagaaggc
     3061 ccaccctgac gtgctcaaca tcctgctcca gatcctggat gacggccgga tcaccgacgc
     3121 ccacggccgg acggtgaact ttgaaaatac agtcatcgtc atgacctcca acgccggcag
     3181 caacaccaaa tcgggctctg tgggcttcgc ccgcaccgcc aatgaacagg gcagggagcg
     3241 ggccatgaag gcccttcagg agttcctgcg gcccgagttc atcaaccggg tagacgagat
     3301 cgtctacttc aaccagctca cagaggacaa cttcaaggcc atcgctgccc tgatgctcca
     3361 ggagctccag gactccctgt ccgagaaggg catcgtcttc acctggcagg acagcctgct
     3421 ggactatctg gtgaaaaagt cctattccgc cgcctacggc gcccgcaacc tgcgccgcct
     3481 gatccagaag gagctggagg atgccatcgc ctcccgcatc atcgacagct ggcagcaccc
     3541 ggtcgcccgg ctggatgcct cctccgacgg cgagcagctg gtcctgtccg ccctgtaaag
     3601 atccctaagt ccttttgcgt ccctctggcg gcgctgccgc cgccatcttt ctccccaaga
     3661 gcgtgtgggc acaaaaaggg gcccggaacg acatcaaaaa acgcccggca gtctgtgact
     3721 gccgggcgtt ttttgttttc aggaccgcgc tcagcccttg gcgatggcgg cggtatccat
     3781 agcgatcata agctcctcat tggtggggat cagcagcacc ttcaccttgg agtcggcggc
     3841 ggagatgacg gtctccttcc cccggacact gttggcctcg gcgtccatct tgacccccat
     3901 gaactccagg tcggaagcga tggccaggcg ctgggaggcg gagttctcgc ccacgccggc
     3961 ggtgaagatg atggcatcca cgccgcccat ggcggcggca tatgcgccaa tgtacttctt
     4021 caccccgtag ttgaacatat ccaccgccag gccagcccgc tcgttgcctt ccttgtgggc
     4081 gttctccagg tcacggaagt cggaggacac gccggacacg ccctgcacgc cggacttctt
     4141 gttcaggata gacaccatct ccttgatgtc atagccgtac ttgcccatga tatactccag
     4201 gatgccggcg tccaggtcgc cggagcgggt gcccatgggg agtccggcca gaggggtgaa
     4261 gcccatggtg gtgtccacgc tcttgccccc atcgatagcg gtgacggagg agccattgcc
     4321 caggtggcag gagatcagct tgagctcctc gatgggcttg cccagcatgg cggcggcgcg
     4381 gccggctacg tacttgtggc tggtgccgtg gaagccgtag cggcggacct tgtccttctc
     4441 atagtactcg tagggcagcg cgtacatgta agccttggcg ggcatggtct ggtggaaggc
     4501 ggtgtcgaac acggccacct gaggcacgtc cttgcccatg acggcggtgc aggcgttgat
     4561 gccggtgatg ttggcggggt tgtggagagg cgccaggggg atgcagtcct ccagagcctt
     4621 catcacctcg tcggtgatga ggacagactg gttgaaagcc tcgccgccgt ggaccacccg
     4681 gtgacccacc gcgtcgatct ccttcatgga gccgatcacg ccgttgtcct tgtccaccag
     4741 ggcgtccagc acagcctgga tggcctcaga gtgggtgggc atggcaatgt cgatagcgtc
     4801 cagcacctgc ttgccctcca tctggggctt ataggtgaac ttgccgtcga tgccgatgcg
     4861 ctcgcaaagg cccttggcca gcacctgctg ggtctcgggg ttgagcagct gatacttcag
     4921 ggaggagctg ccggcgttga tgaccagaat attcatggga aacgatctcc ttttcacagt
     4981 ttttgttgcc tatctgggcg gaatgtacta aactgtatct gtacctgatg gaatcattct
     5041 actgcttttt cccctggagg acaaccattt ttttggattt catcatacat tttttcaagc
     5101 atctccatac agacatccta tcagacaggg ggcgttccgc ttgaaaactg ccggtatcgt
     5161 agccgaatac aatcccttcc acactggcca cgcctatcag atcgcccgca cccgggggct
     5221 cctgggtgcg gactgcgccg tcgtcgctgt catgagcggc aactgggtcc agcggggcgc
     5281 ctgcgccgca gcggacaagt ggacccgggc gggctgggcc ctggccgggg gggcggacct
     5341 ggtgctggag ctgcccacag tgtgggcggc ctccgccgcc gagtcctttg ctacgggcgc
     5401 cgtcgccctg ctccgggcct gcggcgtggt ggatgtcctc tccttcggca gcgagagtgg
     5461 ggacccggcc gccctggccg cggcggccgc ctgcctggac agcgaggcat accgggcggg
     5521 cctgagccgc tttctggacg aagggatgcc cttcgccgcc tgccgccagg ctgtggtggc
     5581 ggccctgctg ggatcggagc gggcgaaggt cctctcccgt cccaatgaca acctgggggt
     5641 ggagtacctg cgggccgcct ccgccctggg ctggtccccc caggtgctgg cggtcccccg
     5701 ccagggagcg ggccacgacg ccccgcgccc tgcggagggc ttcgcctccg cctccatcct
     5761 ccgggagtgg atcgccgccg gccggcggga cctggcagac tgtttcctgc ccgccccatg
     5821 gccggaggag ctggagccca cctccctgtc ccacctggag cgtgccctcc tggcccgtgt
     5881 ccgctctctg tcccaggcgg agtgggccct cctcccggac tccggtgtgg aggaggggct
     5941 tcctgcccgg ctcgtccagg cgggaagcag ggccctgtct gtggaggaat ttctcaccct
     6001 ggccaagacc aagcggtact cccacgcccg cctgcgccgt ctcctgctgt gggccttcct
     6061 gggcctgacc gccgccgacc gcccggctgc cccgccctac ctccgggttc tgggcttcac
     6121 cccacggggg caggagctcc tgcgggccat gaagggagcg gcctccctcc ccatcctcac
     6181 caagccggcc cacgcccggt ccctggacgg gccgggccgc agactcttcc agctggaggc
     6241 ccgctgcacc gacctgtacg gcctgtgttt ctccactccc cgccccggcg gactggagtg
     6301 gaccaccggc cccgtcctga tctgacccgt cccaggaggt cccatggaaa ccaaggaaaa
     6361 aaccacccgc cgcctgcgtc cgctcctacc cctgctcccc cctgtcctgc tctctcttct
     6421 cttctacctg tttcggggcc gtcaggccct gatggacggc tgggtcatcc atgtcatgac
     6481 accggcggag cagcttctgg gccgcctgtc cgccccgctc cccttctccc tggcggaggc
     6541 gctggtcacg gtctttctca ccgcctgcgc tctctgcctc atccgcggca cggtgctggc
     6601 cgtccggctc cacgctccgc atgagctggg ccggcggctg gtctgcctgc tctgtgccct
     6661 gctttgggta tggaccggct actgctggct ttggtctgcg ggctactact ccgcctcctt
     6721 cgcagaaaag agcggtctgg agacccggcc ctacaccgtg tccaccctgg cccaggtcac
     6781 gctgtacttt gcctgccagg ccgcagacct ctccacccag atggtc
//
LOCUS       DBMDSJ010000043         6689 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3383_length_6689_cov_1.763661, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000043 DBMDSJ010000000
VERSION     DBMDSJ010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3383_length_6689_cov_1.763661"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(143..1258)
                     /gene="recA"
                     /locus_tag="ACZPAQ_01880"
     CDS             complement(143..1258)
                     /gene="recA"
                     /locus_tag="ACZPAQ_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR0902757.1"
                     /translation="MAEKKKSVESASPASDKKKALETAMAQIERAYGKGSIMRLGENA
                     GVVVDAIPTGSLSLDIALGIGGVPKGRIIEIYGPESSGKTTLALHIVAEAQKRGGEVA
                     FIDAEHALDPSYARALGVDIDSMLISQPDTGEQGLEICEALVRSGAIDVVVVDSVAAL
                     VPRAEIEGDMGDSHVGLLARLMSQALRKLAGSIAKTNCIVIFINQLREKVGVMYGNPE
                     VTTGGRALKFYASVRIDVRRVEALKNGGEVIGNRTRAKIVKNKVSPPFREAEFDIMYG
                     EGISKWGELVDLAVKLDILQKSGSWFSMGETRLGQGRDAVKEYLKNNPELADRIEAEV
                     RANSFKLMSKQSQIAARAAGRAVDVSADDFEDGADGQ"
     gene            complement(1325..2140)
                     /locus_tag="ACZPAQ_01885"
     CDS             complement(1325..2140)
                     /locus_tag="ACZPAQ_01885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR0902758.1"
                     /translation="MIGGAALAGTAVLTAALWQGLTLRRYALHTDKLSAPVRLLLLTD
                     LHSTFYGRAQRGLLSAIQAQTPDAVLLAGDMADDSRGDAGADALMSALGGAYPCCYVS
                     GNHEVRTGCLEALKRRFRSWGITVLEGSGTLLATPGGTLRICGVDDPSAFAGESGWLE
                     QFRACQALTGDSRYTVLLSHRPERTECYRDSGFDLVVSGHAHGGQVRLPGMINGLFAP
                     HQGAFPDYAGGLYALGSTTLVVSRGLCRNLLPRVFNPPELVALDLLPAEDKAL"
     gene            complement(2167..3030)
                     /gene="prmC"
                     /locus_tag="ACZPAQ_01890"
     CDS             complement(2167..3030)
                     /gene="prmC"
                     /locus_tag="ACZPAQ_01890"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR0902759.1"
                     /translation="MATTYNNLYLDTRARLRKAGIDAAQLEARELLCYASDKSREQLY
                     RDMSLYVSAELERRVEELVQRRLAGEPVAYIVGEWEFYGLPLDISSDVLIPRSDTEVL
                     AERAILRARSAGEGGRVLDLCAGSGCVGLAVAANVPACRVVLGELSEGALRVCKQNVR
                     RNSLNARVTCVSVNALEPPASALWDFDIIACNPPYIPTGDIPGLDVSVRDYEPHMALD
                     GGADGLDFYRAVTQKWKSALRLGGSLIFEVGIGQAPSVEDILARNGFEGIQTTADTQG
                     IWRVVEGTVNR"
     gene            complement(3212..4216)
                     /locus_tag="ACZPAQ_01895"
     CDS             complement(3212..4216)
                     /locus_tag="ACZPAQ_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR0902760.1"
                     /translation="MQEKQEETQQNCARGSCSKGFKTMCGGQALIEGIMMRGPKKESV
                     VVRRPDGGLEIQERELRFIRDRYPVLGWPLIRGVVNFGGSMYSGVKALMYSAEFFPED
                     GEAEEEEPSKFEVWLESRLGSEKAASFFLTLAVILGVGMSIGLFFLLPTLLGTAVTLF
                     THSMLVRNVAESLLKIVIFVGYLVLCSRMKDIHRVFQYHGAEHKTIFCYEAGLPLTVE
                     NVRRQPRHHPRCGTSFLFMVIAISILVSTVVFAIWPVHNPILRFLAHLAMLPLIVGIS
                     YEFNRWAGRHDGPITRILVAPGLWLQNFTTFEPDDSMMEVGIKALELVLPEKQGEDAW
                     "
     gene            complement(4301..5731)
                     /locus_tag="ACZPAQ_01900"
     CDS             complement(4301..5731)
                     /locus_tag="ACZPAQ_01900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902761.1"
                     /translation="MAKKTVIQDRVKIRGIRKRWLVNSMGVVTLILLLAMGTLTLSLW
                     AYYEGSMRDNLSSRASDTARSFSVYTRREYRTAAQDYVAKFPDKQKLEVQFIETDGSL
                     LCTSYGLTTSGTMPGTPDITGAMESGDVKPWTGQDPQTGERIMAVSAPIIYQGTTVGV
                     IRFVTSMAAVDRQLFLSIGVCTLIGIAILTMVYFSNLYFVRSIVEPLASINETARLIA
                     DGSYGIQIEKKFDDEIGELTDTINDMSLKIKQSEKTQSEFISSVSHELRTPLTAITGW
                     AETIQSGELKDPGDVRKGMDIIVSEAHRLTNMVEELLEFSRIEDGRFTLSVEPLDLKA
                     ELEDAVYTYREFFRKEGIALEYTECPEEMIPISGDPGRLRQVFCNLLDNAAKHGGSGK
                     RIDVSIAQEGGEGVIRIRDYGPGIPPEELPHVKYKFYKGSSKARGSGIGLAVCEEIIT
                     RHEGTLDIGNAEGGGCLVTIRLPIET"
     gene            complement(5999..6688)
                     /locus_tag="ACZPAQ_01905"
     CDS             complement(5999..6688)
                     /locus_tag="ACZPAQ_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0902762.1"
                     /translation="MKKILVLEDEENIRSFVVINLKRAGYETVEAGTGEEALAQLKQN
                     PGIRVALLDIMLPDIDGFEVCRRIRAMDNKIGIIMLTARTQEMDKVTGLMTGADDYVT
                     KPFSPAELTARVDALFRRTGGDEELEADDEVVQPPFRLNTRNRTLEKNGQRVKLTQVE
                     YGIVKLFMENPGKALSREEILDAVWGRDYFGELKIVDVNIRRLRIKIEDDATNPVYIT
                     TVWGYGYKWGG"
BASE COUNT         1141 a         2326 c         1941 g         1281 t
ORIGIN      
        1 cctccgacag ctccttgccg gcatagaggg cgaagtccac cacctcgccc tcccccagcc
       61 ggaggatgga gccgtcctcc agcaccgcca gccagcggcc ctccacacgc cgggagggct
      121 tcagatcctg gatgaccacg gcttactgcc cgtccgcccc gtcctcaaag tcgtcggcgg
      181 acacgtccac cgcccggcct gcggccctgg cggcgatctg ggactgtttg ctcatcagct
      241 tgaaggagtt ggcccgcacc tcggcctcga tgcggtcggc cagctccggg ttatttttca
      301 ggtactcctt caccgcatcc cggccctggc ccaggcgggt ctcccccatg gagaaccatg
      361 agccggactt ctgaagaatg tccagcttga cggccagatc caccagctcg ccccacttgg
      421 agatgccctc gccgtacatg atgtcgaact ccgcctcccg gaaggggggg gacaccttgt
      481 tcttcacgat cttggcccgg gtgcggttgc cgatgacctc gccgccgttc ttcagtgcct
      541 ccacccggcg cacgtcgatg cgcaccgagg cgtagaactt cagcgcccgg ccgccggtgg
      601 tcacctccgg attgccgtac atgaccccta ccttttcccg cagctggttg atgaagatga
      661 cgatgcagtt ggtcttggcg atggagccgg ccagcttccg cagggcctgg ctcatcagcc
      721 gggccagcag gcctacatgg ctgtccccca tgtccccctc gatctccgcc cggggcacca
      781 gggcggctac cgagtccacc accaccacgt cgatggcacc ggaccggacc agggcctcac
      841 agatctccag cccctgctcg ccggtgtctg gctgggagat gagcatggag tcgatgtcca
      901 cccccagggc gcgggcatag gaggggtcca gggcgtgctc cgcgtcgatg aaggccacct
      961 cgcccccgcg cttctgggcc tccgccacga tgtgcagggc cagggtggtc ttaccggagg
     1021 actccggccc gtagatctca atgatgcgcc ccttgggcac gccgccgatc cccagggcga
     1081 tgtccaggga cagggagccg gtggggatgg cgtccaccac cacccctgcg ttctctccca
     1141 ggcgcatgat ggagcccttg ccataggccc gctcgatctg ggccatggcg gtctccagag
     1201 ccttcttctt atcagaggcg ggggaggcag actccacgct ctttttcttt tcagccattc
     1261 tgatcgatcc tttccatccc cgggggcggc gcagccgtct ccgggcccac ttttctctta
     1321 aggttcacag cgccttgtcc tctgcgggca gcagatccag cgccaccagc tcagggggat
     1381 tgaacacccg gggcagcagg ttccggcaga gcccccggct caccaccagg gtggtgctcc
     1441 ccagggcgta gagcccgccg gcgtagtccg ggaaggcccc ctggtgcggg gcgaacaggc
     1501 cgttgatcat ccccggcagg cggacctgcc cgccatgggc atggcccgac accaccagat
     1561 cgaatccact gtcccggtaa cattccgtcc gctccggccg gtgggagagc agcactgtgt
     1621 accggctgtc ccccgtcagc gcctggcagg cccggaactg ctccagccac cccgactcgc
     1681 cggcaaaggc ggaggggtcg tccaccccgc agatccggag tgtgcccccc ggcgtcgcca
     1741 acagagtgcc gctgccctcc agcacggtga tcccccagct ccggaagcgc ctcttcagcg
     1801 cctccaaaca gccggtgcgc acctcatggt tgcccgacac atagcagcag ggatatgccc
     1861 cacccagggc ggacatcagg gcatccgccc cggcgtcgcc ccgggagtcg tctgccatgt
     1921 cccccgccag gaggacggcg tcgggcgtct gggcctggat ggcggagagc agcccccgct
     1981 gcgcccgtcc ataaaatgtg ctgtgcaggt cggtgagcag cagcaggcgc actggggcgg
     2041 aaagcttgtc cgtatgcaag gcgtagcggc gcagagtcag tccctgccac agggcggccg
     2101 tcaggaccgc ggtccccgcc agcgccgccc cgcctatcat ccatttcttc gtctttccat
     2161 atgccattaa cggttcacgg tcccctctac cacccgccag atcccctggg tgtctgcggt
     2221 ggtctgtatg ccttcaaagc cgtttcgggc caggatgtcc tccacgctgg gggcctgtcc
     2281 gatgcccacc tcaaagatca ggctaccccc cagccgcagg gcgcttttcc acttctgggt
     2341 cacggcccgg taaaaatcca ggccgtccgc cccgccgtcc agggccatat ggggctcata
     2401 gtcccgcacg gagacatcca ggcccgggat gtctccagtg gggatatagg gtgggttgca
     2461 ggcgatgatg tcaaagtccc acagggcaga ggccgggggc tccagggcgt tgacgctgac
     2521 acaggtgacc cgggcgttca ggctgttgcg gcggacgttc tgcttgcaca cccgcaaggc
     2581 tccttccgac agctccccca gcaccacccg gcaggcgggc acattggcgg ccaccgccag
     2641 ccccacgcag ccgctgccgg cgcacaggtc cagcacccgg cctccctcgc ctgccgatct
     2701 ggcacggagg atggcccgtt cggccagcac ctctgtgtca cttctgggga tgagcacgtc
     2761 gctggagata tccagaggga ggccatagaa ctcccactcc cccacgatat aggctaccgg
     2821 ctcccccgcc aggcggcgct ggaccagctc ctccacccgc cgctccagct ccgccgaaac
     2881 atagagggac atgtcccggt acagctgttc ccggctcttg tcagaggcat agcacagcag
     2941 ttcccgggcc tccagctggg cggcgtcgat gcctgccttt ctcagccgcg cccgggtgtc
     3001 caggtatagg ttgttatagg tcgtcgccat tcagctcccc ccattttttc aattaggaat
     3061 gaggaattat tttgtccagc tccgccgcac acggtccggt cctgattccc cgccacgctc
     3121 tccaaggctc ccgcagcagg acccccccct ccagctggaa ggctccccac aacagctaaa
     3181 attcctaatt caaccaagcg cctccggtgc cttaccaagc gtcctcgccc tgcttctccg
     3241 gcaggaccag ctccagcgct ttgatgccca cttccatcat ggagtcgtcc ggctcaaagg
     3301 tagtgaagtt ctgcagccac aggcccgggg ccaccaggat gcgggtgatg gggccgtcgt
     3361 gccgtccggc ccagcggttg aactcgtagc tgatgcccac gatcaggggc agcatggcca
     3421 gatgggccag gaagcgcagg atcgggttgt gcaccggcca gatggcgaac accacggtgg
     3481 acaccaagat ggagatggcg atcaccatga acaggaagct ggtgccgcat cggggatggt
     3541 gcctgggctg cctgcgcacg ttctccaccg tcaggggcag gcccgcctca tagcagaaga
     3601 tagtcttgtg ttccgccccg tggtactgga acacccggtg gatgtctttc atccgggagc
     3661 agagtaccag atagcccaca aaaatcacga tcttcagcag gctctccgcc acgttgcgca
     3721 ccagcatgga gtgtgtgaac agggtcacgg cagtgcccag cagggtgggc aggaggaaaa
     3781 acaagccgat ggacatgccc acgcccaaga tgaccgcaag agtcaggaaa aaggaggccg
     3841 ccttctcgct gcccagcctg ctctccagcc atacctcgaa cttggagggc tcctcctctt
     3901 ccgcctcccc gtcctcgggg aaaaattccg cggagtacat cagcgccttg acgccgctgt
     3961 acatagagcc gccgaagttg actactcccc ggatcagggg ccagcccagc accggatagc
     4021 ggtcccggat gaatctgagc tccctctcct ggatctccag tccgccgtca gggcggcgga
     4081 ccactacgga ctcctttttc gggccccgca tcatgatgcc ctcgatgagg gcctggccgc
     4141 cgcacatggt cttgaagccc ttggagcagc tgccccgggc acagttctgc tgtgtttctt
     4201 cttgcttttc ttgcatggat cttggttctc cttttcattg aaggacaggc tcagcactat
     4261 tctaccaaat cagggccgga atagcaaggg ggaatagaac ttatgtttca atcggcagcc
     4321 ggatggtcac caggcagccg ccgccctccg cgtttccgat gtccagggtg ccctcgtgcc
     4381 gggtgatgat ctcctcacac accgccagac caatgccgga accccgggct ttcgagctgc
     4441 ccttatagaa tttgtacttt acatggggga gctcctccgg cgggatgccg gggccgtagt
     4501 cccggatgcg gatgacgccc tctcccccct cctgggcgat ggacacgtcg atacgctttc
     4561 cgctgccgcc gtgcttggcc gcattgtcca gcagattgca gaacacctga cgcagccgcc
     4621 ctgggtctcc ggagatgggg atcatctcct ccgggcactc ggtatactcc agagcgatgc
     4681 cctccttgcg gaagaactcc ctgtaggtgt acaccgcgtc ctccagttcc gctttcagat
     4741 ccagcggctc caccgacagg gtgaagcggc cgtcctcaat gcgggagaac tccagcagct
     4801 cctccaccat gttggtcagc cggtgggcct ccgacacgat gatgtccatc cctttgcgca
     4861 cgtcgcccgg gtccttcagc tcaccgctct gaatcgtctc cgcccagccg gtgatggcgg
     4921 tgagaggggt gcgcagctcg tgggagacgg aagaaataaa ctccgattgg gtcttttccg
     4981 actgcttgat cttcagagac atgtcattga tggtgtctgt gagctctccg atctcgtcat
     5041 cgaacttctt ctcgatctgg atgccgtagc tgccgtccgc aatgaggcgg gccgtctcgt
     5101 tgatgctggc cagcggctcc acgatggaac gcacgaaata caggttagag aaatacacca
     5161 tagtcaaaat ggcgatcccg atcagggtgc atacaccgat ggacaggaac agctgccggt
     5221 ccaccgccgc catagaggtg acgaaccgga tcacgcccac tgtggtcccc tggtagatga
     5281 tcggggcgga cacggccatg atccgctccc ctgtctgggg gtcctgtccc gtccagggct
     5341 tcacatcgcc gctctccatg gcgccggtga tgtccggcgt ccccggcatc gtgcccgagg
     5401 tggtcaggcc gtagctggta cacagcagcg aaccgtctgt ctcgatgaac tgcacctcca
     5461 acttctgctt atcgggaaat tttgctacat agtcctgggc tgccgtccgg tactcccgcc
     5521 gggtgtagac gctgaagctc cgggccgtgt cagaggcccg ggaggagagg ttgtcccgca
     5581 tggacccctc atagtacgcc cacagggaca aggtgagcgt tcccatggcc agcagcagga
     5641 tcagggtcac cacgcccatg ctgttcacca gccagcgctt ccggatgccc cggatcttca
     5701 cccggtcctg gatcactgtc ttcttcgcca ccgtatccct cctctccgtt caggggccta
     5761 tccccgctgt tttcttcgct ttatccgctc cacttgtacc catatcccca cacggtggtg
     5821 atataaaccg ggttcgtggc gtcccccccc aaagagctcg ctctttgggg acccctcttt
     5881 tttccgatgc tcggggcaaa tgaattgccc ctccgccaat gcttcgcact gcgaagcatt
     5941 gacgcgctgc cgcgcgcccc gctctgcggg gccccggacg acgcccctgg gagcctgctt
     6001 atccgcccca cttgtaccca tatccccaca cggtggtgat ataaaccggg ttcgtggcgt
     6061 cgtcctctat cttgatgcgg aggcggcgga tgttcacatc cacgatcttc agctctccaa
     6121 agtagtcccg cccccacacc gcgtccagga tctcttcccg ggacagtgcc ttgcccgggt
     6181 tctccatgaa cagcttcacg atgccgtact ccacctgagt cagcttcacc cgctgtccgt
     6241 ttttctccag ggtccggttg cgggtattca gccggaaggg gggctggacc acctcgtcat
     6301 ctgcctccag ctcctcatcc cctccagtgc ggcggaacag ggcatccacc cgggcggtaa
     6361 gctccgccgg ggagaaaggc ttagttacat agtcgtccgc cccggtcatc aggccggtca
     6421 ccttgtccat ctcttgggtc cgggccgtca gcatgatgat cccgatctta ttgtccatgg
     6481 cccggatgcg ccggcacacc tcaaagccgt cgatatcggg cagcatgatg tccagcaggg
     6541 ccaccctgat gccagggttc tgcttcagct gggccagggc ctcctctccg gtgcccgcct
     6601 ccacagtctc gtagcccgcc cgcttcaggt tgatgaccac aaagctgcgg atattctctt
     6661 cgtcctccaa caccaggatc tttttcatt
//
LOCUS       DBMDSJ010000044         6610 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3421_length_6610_cov_1.890920, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000044 DBMDSJ010000000
VERSION     DBMDSJ010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_6610_cov_1.890920"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="ACZPAQ_01910"
     CDS             complement(<1..252)
                     /locus_tag="ACZPAQ_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902763.1"
                     /translation="MVTRILSAVVLAPLFFVVLFFLPPVFLAVLLACITARASFELLR
                     ATKVAHHNGMYVYTAAAAGLIPLTFWLGWGNWSLAALSLL"
     gene            complement(256..1002)
                     /locus_tag="ACZPAQ_01915"
     CDS             complement(256..1002)
                     /locus_tag="ACZPAQ_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR0902764.1"
                     /translation="MPLFTKKPQPEVQVDFQDLPRHIAIIMDGNGRWAKKRGLPRSAG
                     HAAGAETFRTIATYCKEIGLEYLTVYAFSTENWKRPEDEVGAIMGLLKKYLLEAIGQM
                     ERDKVKMEFFGDLSPLSQELRDLCEQTRRISKRYDGCQVNICLNYGGRDEILRAARAY
                     ALDCVEGRADPNHLTQENFSGYLFSRGVPDPDLIIRPSGELRLSNFLLWESAYAEFYF
                     TDVLWPDFSKAELLRAIASYQSRARRFGGI"
     gene            complement(1107..1661)
                     /gene="frr"
                     /locus_tag="ACZPAQ_01920"
     CDS             complement(1107..1661)
                     /gene="frr"
                     /locus_tag="ACZPAQ_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR0902765.1"
                     /translation="MSPETKVYDAKMQKTIEVVKANFASVRAGRANAGVLDRIFVEYY
                     GTPTPLNQVAAISSPDPRTLMIQPWDGTLLKAIEKAIQTSDLGINPQNDGRVIRLGFP
                     QLTEERRRDLTKQVHKYGEEGKVALRNIRREAMDEIKKQVKKSEITEDDQKQLEKELQ
                     DLTDKRCKEVDELTAKKEQELMAV"
     gene            complement(1701..2417)
                     /gene="pyrH"
                     /locus_tag="ACZPAQ_01925"
     CDS             complement(1701..2417)
                     /gene="pyrH"
                     /locus_tag="ACZPAQ_01925"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR0902766.1"
                     /translation="MEQPKYKRVLLKISGEALAGDASRGLDFNVIGQVCEVIKECTQL
                     GVQVGVVVGGGNFWRGLKDGGDRMERVRADHMGMLATSINALALADVLEQKGVDVRVQ
                     TAIEMRAVAEPYIRSRAIRHLDKGRVVIFGCGTGNPFFSTDTAAVLRAAEIDADVILL
                     AKNIDGVYSADPKVDPKAVKYDTITYDDVLAQHLQVMDSTATSLSMDNKIPVLLFALK
                     DPQNIIRAVMGEKIGTIVKG"
     gene            2627..3406
                     /locus_tag="ACZPAQ_01930"
     CDS             2627..3406
                     /locus_tag="ACZPAQ_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR0902767.1"
                     /translation="MAQALLWAAGGTGFTALLTALGAATVFFFRSRPDPTVHRIFLGF
                     AAGVMIAASMWSLLIPAIEEAEAMGMVGWVPAAGGSVLGIAFLLAMDALLPQLTPESF
                     RHLGRRTPLLVLAVTLHNIPEGMAVGISFALASQGDTPAQLTAAVALALGIGIQNFPE
                     GAAIALPLRQEGLSRAKSFWMGALSGVVEPIFALLTVLMVGSLQPLMPWLLSFAAGAM
                     LYVVVEELIPGAHLEDHSSRGGTLGVMAGFLLMMILDVALG"
     gene            complement(3443..4213)
                     /gene="sigG"
                     /locus_tag="ACZPAQ_01935"
     CDS             complement(3443..4213)
                     /gene="sigG"
                     /locus_tag="ACZPAQ_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HIR0902768.1"
                     /translation="MQNKVEICGVNTSKLKVLKNDETQALLLRAKQGDMKAREELIAG
                     NLRLVLSVIQRFANRGENADDLFQVGCIGLIKAIDNFNTDLDVRFSTYGVPMIVGEIR
                     RYLRDNSTMRVSRAMRDTAYKVLQAKEAYMAEHQREPTVDEIARMLELKREDVVFALD
                     AIVDPVSLYEPVYSDSGDTICVMDQVRDSKNTDENWLEQIALREAIAKLSDREKRILS
                     LRFFQGKTQMEVSTEVGISQAQVSRLEKNALRQIKKDL"
     gene            complement(4417..4932)
                     /locus_tag="ACZPAQ_01940"
     CDS             complement(4417..4932)
                     /locus_tag="ACZPAQ_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0902769.1"
                     /translation="MIGEKTRKLVYAALFAALTCVMTMIVKIPVPATGGYVNLGDCVV
                     LLAGWVLGPMYGGAAAGLGSMLADLVSGYPLYAPGTFVIKACMAVCAWAVFRAAAGEK
                     RGLAARIGGALAGEAVMVAGYFAYEGVVLGFGLGAAAAIPGNLIQGVFGLAVSVAAAE
                     ALSRSGAVKEL"
     gene            5070..6464
                     /gene="pdxR"
                     /locus_tag="ACZPAQ_01945"
     CDS             5070..6464
                     /gene="pdxR"
                     /locus_tag="ACZPAQ_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR0902770.1"
                     /translation="MLTYDLDRRGTIPRYDYLCRCIRNDILCGRLTAGSRLPSRRSLA
                     EHLKVSAVTVESAYAQLAAEGYIVSREKSGYFVSQLEPALRPPSPPPIRTDAPPPGQP
                     WMMDLRGGGGSTELFPFSVWAKLMRQVLSQRGPDLLRPVPHSGVPELREAIAGTLYRL
                     RGLSVSPEQIVVGSGSEYLYNLIVQLLGRELVYGVEDPGYPKPARIYALNGARCISLP
                     VDGQGISPQTLAASPAQVVHLSPSHQFPTGAVTPAGRRQALLRWAAQGGDRYLIEDDY
                     DSEFRFTGLPIPALQSIDQADRVIYLNTFSRALAPSLRISYLVLPPTLLDRYHQRLGF
                     YSSTVPALEQYTLASFLSEGRFEQHLSRARNRYRALRDQVLEALEASGLAGRAQISGA
                     DAGLHFLVRLETERGDQELTQLARARGIRLSFLSEFCASPGPEHQHFLVISYPGIQME
                     RLPQTLRELAVLLH"
BASE COUNT         1104 a         2253 c         1925 g         1328 t
ORIGIN      
        1 cagcagggac agggcagcca gggaccagtt cccccacccc agccagaagg tcagggggat
       61 caggccggcc gccgccgccg tgtagacata cattccattg tggtgggcca ccttggtggc
      121 cctcagcagc tcaaaggacg cccgcgcggt gatgcatgcc agcagtaccg cgagaaagac
      181 gggcggcaga aagaacagga caacaaaaaa cagcggggcc aatactacag ccgagaggat
      241 gcgcgtcacc atttctcaaa tacctccaaa tcgccgggcc cggctctgat agctggcgat
      301 ggcccgcagc agctctgctt tgctgaaatc cggccacagc acatccgtaa agtagaactc
      361 cgcataggcg gactcccaca gcaggaaatt ggataggcgc agctccccgc tggggcggat
      421 gatcaggtcc gggtccggca ccccgcggga gaacaggtag ccggagaagt tctcctgtgt
      481 caggtggttg gggtccgccc tcccctctac acagtccagg gcataggccc gggcggcccg
      541 gaggatctcg tcccgtccgc cataattcag gcagatattc acctgacagc cgtcgtacct
      601 cttggagatg cgtcgggtct gctcacacag gtcccggagc tcctgggaca gcggagagag
      661 gtccccgaaa aactccatct tcaccttgtc ccgttccatc tgtccgatgg cctccagcag
      721 atacttcttc agcaggccca tgatggcgcc cacctcatcc tccggccgct tccagttttc
      781 cgtggaaaag gcatagaccg tcagatactc caggccgatc tccttgcagt aggtggcgat
      841 ggtgcggaaa gtctccgccc cggcggcatg tcccgccgaa cggggcagcc cccgcttttt
      901 ggcccaccgg ccatttccat ccatgataat ggcgatatgc cggggcaggt cctgaaagtc
      961 aacctgtacc tccggctgag gcttctttgt aaacagcggc atatctgatc ttccttaccc
     1021 aaacaaaatg ctgagagcgc acgtgcagaa tgaagaatga acgtcttgag aacattccgc
     1081 atcctgcatt gtgcagcccg catcgctcag accgccatca gctcctgctc ctttttcgcg
     1141 gtcagctcat ccacctcttt gcaccgcttg tcggtcaggt cctgaagctc cttttccagc
     1201 tgcttctggt catcttcggt gatctcgctc ttcttgacct gttttttgat ctcatccatg
     1261 gcctcgcgcc ggatgttccg aagggccacc ttgccctcct caccgtactt gtgcacctgc
     1321 ttggtcagat cccggcggcg ctcctctgtc agctggggga agcccaggcg gatcacccgg
     1381 ccgtcgttct ggggattgat gcccaggtca gaggtctgga tggccttctc gatcgccttg
     1441 agcagggtgc cgtcccaggg ctggatcatc agggtgcggg ggtcggggga ggagatggcc
     1501 gccacctggt tgagcggggt gggcgtgccg tagtactcca cgaaaatacg gtccagcacg
     1561 ccggcattgg cccgtccggc acggacagag gcgaagttgg ccttgaccac ttcgatggtc
     1621 ttctgcatct tggcatcgta aacttttgtc tcagggctca tcgtcgaatc ttcctttctg
     1681 ttgtcagctg gcgccttggt tcagcctttt acaatggttc cgatcttctc tcccatgacc
     1741 gcccggatga tgttctgagg gtccttcaga gcgaagagca gcaccgggat cttgttgtcc
     1801 atggagagcg aggtggcggt ggagtccatc acctggagat gctgagccag cacgtcgtcg
     1861 taggtgatgg tgtcgtattt gaccgccttg gggtccacct tggggtcggc tgaataaact
     1921 ccgtcgatgt tcttggccag gaggatcaca tcggcgtcga tctccgccgc ccgcaggacc
     1981 gcagctgtgt ccgtggagaa gaaggggttc cccgtgccgc agccgaagat gaccacccgg
     2041 cccttgtcca ggtgccggat ggctctggag cggatatagg gctccgccac cgcccgcatc
     2101 tcgatggcag tctggacccg gacgtccact cccttctgct ccagcacgtc cgccagggcc
     2161 agggcgttga tgctggtggc cagcatcccc atgtggtcgg cccggacccg ttccatccgg
     2221 tcgccgccgt ccttcagccc gcgccagaag tttccgccgc ctaccacgac gcccacctgc
     2281 acgcccagct gggtgcactc cttgatgacc tcgcacacct gtccgatcac gttgaagtcc
     2341 agtccccggg aggcatcccc cgccagggcc tcgccgctga tcttcagcag cacgcgttta
     2401 tactttggct gttccattgg aaacactcct cgcatcaaga tcaatgattc atccctattc
     2461 taatataatt ctcctctttt gcataggggg tacggcaaag tttttcattt tccctggagg
     2521 agcaccggga caccccctcc cgcatagtgt gaagcggggg gagccgtcag tgcgctcccc
     2581 gcctatcaca gcggcgcgtg acccgcgccg ggaaaggaac agtacattgg cacaggcact
     2641 gttatgggcc gccggcggaa cagggttcac cgctctgctc accgccctgg gggcggccac
     2701 cgtatttttc ttccggagcc gccctgaccc cacggtccac cggatcttcc tggggttcgc
     2761 cgccggcgtg atgatcgccg ccagcatgtg gagcctcctg atccccgcca tcgaggaggc
     2821 ggaggccatg ggcatggtgg gatgggtccc ggccgctggg gggtccgtcc tgggcatcgc
     2881 cttcctgctg gccatggacg ccctgctccc ccagctcacc cctgaatcct tccgccatct
     2941 gggccgccgg accccccttc tggtcctggc cgtcactctc cacaacatcc cggaggggat
     3001 ggcggtgggc atctcctttg cgctggcctc ccagggggac acccccgccc agctcaccgc
     3061 cgccgtcgct ctggccctgg gtatcggcat ccagaacttc cccgaggggg ctgccatcgc
     3121 cctgcctctg cgccaggaag ggctcagccg ggccaaatcc ttttggatgg gggccctgtc
     3181 cggcgtcgtg gagcccattt tcgccctgct caccgtcctg atggtgggca gcctccagcc
     3241 gctgatgccc tggctgctct cctttgcagc cggcgccatg ctgtatgtgg tcgtggagga
     3301 gctgatcccg ggcgcccacc tggaggacca ctcctcccgg ggcggaactc tgggggtaat
     3361 ggccgggttc ctgctcatga tgatcctgga cgtagccctg ggctgagtct acaaatatgt
     3421 aaagccgccc tgaggcggct gttcacaggt cctttttgat ctggcgcagg gcgttcttct
     3481 ccaggcggga cacctgggcc tgggagatcc ccacctcggt ggacacctcc atctgggtct
     3541 ttccctgaaa aaaacggagg gacaggatgc gcttctcccg gtccgacagc ttggcaatgg
     3601 cttcccgcag tgcgatctgc tccagccagt tctcgtccgt gtttttgctg tcccgcacct
     3661 gatccatgac acagatggta tccccgctgt cggagtacac cggctcgtag agggagaccg
     3721 ggtccacgat ggcgtccagg gcaaagacca cgtcctcccg cttgagctcc agcatcctgg
     3781 ctatctcgtc tacggttggc tcccgctggt gctcggccat ataggcctcc ttggcctgga
     3841 gcaccttgta cgccgtgtcc cgcatggccc gggacacccg catggtgctg ttatcccgga
     3901 ggtaccgccg gatctccccc acgatcatgg gcacgccata ggtggagaag cgcacgtcca
     3961 gatccgtgtt gaagttgtcg atggccttga tgaggccgat gcaccccacc tggaacaggt
     4021 cgtccgcgtt ctccccccgg ttggcaaagc gctggatcac cgagaggacc agccgcaggt
     4081 tgcccgcgat cagttcctcc cgggccttca tgtccccctg cttggcccgg agcaggaggg
     4141 cctgggtctc atcgtttttc aggaccttca gctttgacgt gtttaccccg cagatctcca
     4201 ccttgttttg catctgttgg aacctcctgc catgatactg tgctttcagt atctccggga
     4261 cagagggttt cataccgcgg ggagcggctt aaaaatttta acgctcgaca gggttcgatg
     4321 gaatttcagg aacagccgga aagcgggaca gcaggcagta cgcgctccgc cccggccccc
     4381 atctggggcc ggggcagggc gcagccgttc aggccgtcac agttccttga ccgcgccgct
     4441 gcgggacagg gcctccgccg cggccaccga gaccgccagg ccgaaaacgc cctggatcag
     4501 gttgcccggg atggcggcag ccgctcccag gccaaatcca aggaccactc cttcataggc
     4561 gaagtagccc gccaccatca ccgcctcccc cgccagggcg ccgccgatgc gggcggccag
     4621 cccccgcttt tctccggctg ccgcccggaa tactgcccag gcgcagaccg ccatgcatgc
     4681 tttgatcaca aaggtaccag gtgcatacag agggtatccg ctgaccaggt cggccagcat
     4741 agagccaagc ccagccgccg cgccgccgta cattggaccc agcacccacc cggccagcag
     4801 caccacgcag tcccccaggt tgacatagcc ccccgtggca ggcactggga tcttgacgat
     4861 catggtcatc acacaggtca gcgccgcaaa cagcgccgcg tacaccagct ttctcgtttt
     4921 ttctccaatc atctcgctct acgctccttt tgtcctgttc acaggaaaaa tgtccttgca
     4981 tttttccgct gtctgtattt taatcttatc tggtctgatg aaaaggtcca gtttcgttct
     5041 tttttatcag gccagatagg agggcgacca tgctcacata tgatctggac cgtcggggga
     5101 ccatcccccg ctatgactac ctctgccgct gcatccggaa tgacatcctc tgcggccgcc
     5161 tgaccgccgg gagccggctc ccctcccggc ggagccttgc ggagcacctg aaggtcagtg
     5221 ccgtcacggt ggagagcgcc tatgctcagc tggctgcgga gggatacatc gtttcccggg
     5281 aaaagagcgg ctatttcgtc tcccagctgg agcccgccct gcgtcccccg tccccgcccc
     5341 ccatccggac cgatgctccg cccccggggc agccctggat gatggatctg cggggaggcg
     5401 gcgggagcac agagctcttt cccttctctg tctgggccaa gctgatgcgg caggtactgt
     5461 cccagcgtgg tccagatctc ctgcgtcccg tgccccacag cggggtccct gagctgcggg
     5521 aggccatcgc cggcaccctg taccgcctgc ggggcctctc cgtctccccg gagcagatcg
     5581 tggtgggctc ggggtccgaa tatctgtata acctcatcgt acagctcctg ggccgggagc
     5641 tggtctatgg cgtggaggac ccgggatacc caaagcccgc caggatctac gccctcaacg
     5701 gggcccgctg catctccctc ccggtggacg gacaggggat ctccccccag accctcgccg
     5761 cctccccggc tcaggtggtc cacctctccc cctcccatca gttccccact ggggccgtca
     5821 ccccggcggg gcggcgtcag gccctgctcc gctgggccgc ccagggcggg gaccgctatc
     5881 tcatcgagga tgactacgac agcgaatttc gctttacagg gcttcccatc cctgccctcc
     5941 agagcatcga ccaggcggac cgggtcatct atttgaacac cttctcccga gctctggccc
     6001 cctccctgcg catcagttat ctggtgctgc ctcccaccct gctggaccgc tatcaccagc
     6061 gcctggggtt ctactcctcc accgtccccg ccctggagca gtacaccctg gcctccttcc
     6121 tctcagaggg gcgcttcgag cagcacctca gccgggcccg gaaccgctac cgggccctcc
     6181 gtgaccaggt gctggaggcc ctggaggcca gcggcctggc cggccgggcc cagatctccg
     6241 gtgcggacgc ggggctccac ttcctggtcc ggctggagac ggagcgcggc gaccaggagc
     6301 tgacccagct ggcccgtgcg cggggcatcc gcctctcctt cctgtcggag ttctgcgcct
     6361 cccccggccc ggagcaccag cactttctgg tcatcagcta cccgggcatc cagatggagc
     6421 gcctgcccca gaccctgagg gagctggctg tgctgctcca ctgagtccgg ggcgtaaaaa
     6481 ctctttagga gggggcaagc aaaaaatctc actccgccca atctcgataa atgataaaaa
     6541 gcgcaggagc tggcgctcct gcgctttttg cagcgtccct tttcccgcct catctgttac
     6601 agcttctgct
//
LOCUS       DBMDSJ010000045         6460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3504_length_6460_cov_1.321906, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000045 DBMDSJ010000000
VERSION     DBMDSJ010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_6460_cov_1.321906"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            133..1371
                     /gene="dinB"
                     /locus_tag="ACZPAQ_01950"
     CDS             133..1371
                     /gene="dinB"
                     /locus_tag="ACZPAQ_01950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR0902771.1"
                     /translation="MDRVIFHCDLNSFYASVELLDHPELRHLPVAVCGDPASRHGIIL
                     AKNEPAKAFGIKTAETIWQARKKCPRLVLLPAHHDKYREFSRQVNQLYQEYTDLVEPF
                     GIDESWLDVTGSLHLFGGDPLALAHQLRRRVRGELGLTISVGVSFNKVFAKLGSDYKK
                     PDAVTLISPDNFRALVWPLPVTDLLYVGRAAADTLKRFGVRTIGDLARFDREALFSLL
                     GRHGRQLHAYANGLDRSPVSSACQENAPKSIGNGLTFPRSLSGREEICSGIAMLSDRV
                     AVRLRRAGMKAAGVSLAIRDPDFRDISRQRRLEPPTCLARELSPAAVSLAEGCWNMDS
                     PVRALTVTALYLISEDQAGVQLDLLGQADAPRRDRLERLDRTLDQIRAKYGRNAISPA
                     SSAGVCQGGVQEEDEEGATS"
     gene            complement(1587..1772)
                     /locus_tag="ACZPAQ_01955"
     CDS             complement(1587..1772)
                     /locus_tag="ACZPAQ_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR0902772.1"
                     /translation="MAVKMLLIAILIAVLMTLFSGIYPGSVLYGWLDQNGLNFVWAGL
                     TAGIIAACLVLYRRRKK"
     gene            complement(1939..2475)
                     /locus_tag="ACZPAQ_01960"
     CDS             complement(1939..2475)
                     /locus_tag="ACZPAQ_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902773.1"
                     /translation="MKQNKKWSMRIASFCLAGACLCGGAVLAAGGDESDPLVTLSYLT
                     QTVTPNILKQVDEQAAKRQSELLAQLNTAIADYKMAVEGGGASGGTSASYSVVTLTSG
                     QTLALGVGCEVMLRVGTATLAANTDPGLIDVSTGGTLNNGGALTANHLYMATIADRTV
                     QATAATVKLLVRGEYTVS"
     gene            complement(2580..2948)
                     /locus_tag="ACZPAQ_01965"
     CDS             complement(2580..2948)
                     /locus_tag="ACZPAQ_01965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR0902774.1"
                     /translation="MDNSVVKRGDIFYADLSPVVGSEQGGVRPVLIVQNDTGNRHSPT
                     VIAAAITSQTGKARLPTHISLAAMSCGLPKDSVVLLEQIRTLDKRRLREHMGRVDETM
                     MKQVDTAIAVSFGLHPDTMV"
     gene            complement(3149..4291)
                     /gene="alr"
                     /locus_tag="ACZPAQ_01970"
     CDS             complement(3149..4291)
                     /gene="alr"
                     /locus_tag="ACZPAQ_01970"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR0902775.1"
                     /translation="MTETAQMRTWAEISLDRLAHNYQTLRAMLAPDCRFLGLCKANAY
                     GHGALQVGRKLEALGADMLAVACVDEAAELRRGGVTIPILCLGQTPPQLAELLLEHDV
                     TQTVSDLETGRALARAAQAAGRTLKIHVKVDTGMTRLGFLWREGGERETAAAIAALCA
                     LPGLEAEGLFTHFADADGNEEYTMGQFTRFLDARQALEALGVRFKICHCAASAAVLNY
                     PCTHMDMVRPGIALYGYYPAPGLEGLDSSGLLPVMALKTRVAAVRAVPAGTCVSYGCT
                     ARLERDSRLAVLPVGYGDGYPRLLSNRMEVLLRGRRCPVVGRICMDMCMVDVTDVPDA
                     VPGDVAELYGREGLLEQAAALAGTIPYELLCNVNPRVPRIYTGRGE"
     gene            complement(4302..4895)
                     /locus_tag="ACZPAQ_01975"
     CDS             complement(4302..4895)
                     /locus_tag="ACZPAQ_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902776.1"
                     /translation="MRRSLLICVLMMTLSLSACGGGGGGSEAEELALTIRGEYLAMTS
                     CAAQAVVTADYGQRVYRYELSAAVNGEEALFTLTAPETVAGLTARIEEDEGWLEYDGA
                     ILETGELAPGGLTPMGAIPALLETARSGYLDTCVLEELGEVQALRVVSRDPEEKQGSG
                     TETTLWFDAATHALVRGEISQDGVCVLQCEFSQFTKE"
     gene            complement(4913..>6460)
                     /locus_tag="ACZPAQ_01980"
     CDS             complement(4913..>6460)
                     /locus_tag="ACZPAQ_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR0902777.1"
                     /translation="AEEVRGLLEGGGPDFIGGADSVTVLGAGAETEEDRRELEALQAL
                     AEEKNQNHAPWVGVLCGPGNNGGDGVAAARILRGMGFRVRAFLVGDRRKMTPDERAME
                     KKLEEAGGVLEEFRPEDRRQMLWLSLCGCLVDALFGVGLSRPVEGDALLAVRLMQGRS
                     PLKRVVSCDIPSGVHADTGAVLGEAVRADRTVTFTCAKPGLYLGEGASCAGLVTVADI
                     GIPHRLIFGPGAQMLFPVGVMGYEDVVLPRRKRDSHKGDYGRVLILAGSVGYTGAPVL
                     AASGAVRGGAGLVSLGVPEEIWPIAAVKCLEAMPFPLPARYEDILRRASSCEGVLIGP
                     GLGRAPRTERLVRSLLEDLKVPVVLDADGINALDGHIDSLDGRRAPTILTPHAGEFAR
                     LTGRPLPLEDPLTAARDFARDHRCVLVLKGHRTVTAAPDGRAWINTTGNPGMSKGGSG
                     DVLAGLILALLGQGWEPELAARDGVYLHGEAGDRCAGSLGEYGMTPGDLVERLPETLE
                     AHVRSGS"
BASE COUNT         1084 a         2335 c         1941 g         1100 t
ORIGIN      
        1 atctccccac ctcctttcct attttcacca tagcatatcc agatcgctcc gtcaagagaa
       61 atcgaacaaa agtttgactt tcatggtcca aagggatata ataaggacat cctaataagg
      121 ggggagcgcc cggtggaccg tgtcatcttt cactgtgatc tgaacagctt ttacgcctcg
      181 gtggagctgt tggaccaccc ggaactgcgc cacctcccag tggcggtgtg cggcgacccg
      241 gccagccgcc acggcatcat ccttgcaaaa aacgagcctg ccaaggcctt cggtatcaag
      301 accgcggaga ccatctggca ggcccggaaa aagtgtccca ggctggttct ccttcccgcc
      361 caccacgaca agtaccggga gttctcccgc caggtaaacc agctctatca ggagtacacc
      421 gatctggtgg aacccttcgg catcgacgag agctggctgg acgtcactgg ctctctccac
      481 ctcttcggcg gggaccccct ggccctggcc caccaactcc gccgtcgggt gcggggggag
      541 ctgggcctga ccatctcggt gggagtctcc ttcaacaagg tgtttgccaa gctgggcagc
      601 gactacaaaa aaccggacgc cgtcaccctg atctcgccgg ataactttcg tgccctggtc
      661 tggcctctgc cggtgaccga cctgctctat gtgggccggg ctgcggcaga caccctgaag
      721 cggttcgggg tgcggaccat cggcgatctg gcccggtttg accgggaagc cctgttctcc
      781 ctgctggggc gccacggcag gcagctccat gcctacgcca acgggctgga ccgcagtccc
      841 gtctcttccg cctgtcagga gaacgcaccc aagtccattg gcaacggcct taccttcccc
      901 cgcagcttgt ccggccggga ggagatctgc tccggcatcg ccatgctgtc cgaccgggtg
      961 gccgtccgcc tgcgccgggc agggatgaag gctgccggcg tctctctggc catccgggac
     1021 ccggatttcc gggatatcag ccgccagcgc cggctggagc cccccacctg cctggcccgg
     1081 gagctgtccc cggccgccgt ctcgctggcg gagggctgct ggaacatgga cagtcccgtc
     1141 cgtgccctga cggtgaccgc cctctatctg atctccgagg accaggctgg cgtccagttg
     1201 gacctgctgg gccaggcaga cgcccccagg cgggaccgcc tggaacgcct ggaccggacg
     1261 ctggaccaga ttcgggccaa gtacggccgg aacgccatct ccccagcctc ctccgccggc
     1321 gtgtgccagg gcggtgtgca ggaggaagac gaggaaggcg caacctcctg accggcccgg
     1381 aaccgctgtc cggcatccgc gaaagaggcc gccgctatgc ggtttcccaa ggagtcattg
     1441 gatcagctcc agcagctcac tcacattcag gcagaggtcc ggcctttcgc tgccatacag
     1501 gggtgctccg caaggaattc caagttttgt ttcatggaag gcccctttta taaaaagggg
     1561 ccttgtcttt catactcata atacgatcac tttttcctcc ggcggtacag gaccaggcag
     1621 gcggctatga tccccgctgt cagtccggcc catacgaagt tcagaccgtt ttggtccagc
     1681 caaccataca ggacagagcc cgggtagatc ccactgaaga gcgtcatcag cacagcgatc
     1741 aatatagcaa tcagaagcat tttaacagcc atagacacgt tctctcctct ctaccggcgt
     1801 ccccctccgc ggggaaaatg cgcctccact ctctgactct cccatctccg gggctttccg
     1861 ttctctctgc ttcgatcata tcagaaaggg cccggcatgt caaagagaca tgccgggccc
     1921 tctgtctgtg ctccagtctc aggagacggt gtactctccc cgcaccagca gcttgacggt
     1981 ggcggcggtg gcctggactg tccggtccgc gatggtggcc atgtaaaggt ggttggcggt
     2041 cagcgcgccg ccgttgttca gcgtcccccc ggtgcttacg tcgatcagcc ccgggtccgt
     2101 gttggccgcc agggtggcgg tgcccacccg cagcatcacc tcgcatccca cccccagggc
     2161 cagcgtctgc ccggaggtga gagtaaccac ggagtaggag gcactggtcc ccccggaggc
     2221 cccgccgccc tccactgcca tcttatagtc cgcgatggcg gtattgagct gagccagcag
     2281 ctcactctgc cgcttggccg cctgctcgtc cacctgcttg aggatattgg gggtgactgt
     2341 ctgggtgaga tagctgaggg tgaccagggg gtcggactca tctccccccg ccgccaggac
     2401 cgcgccgccg cagaggcagg caccggccag gcagaaggag gcgatccgca tgctccattt
     2461 cttgttctgc ttcatcctat gtactccttt tctgctcccc cgcagcccgg ccggggccgg
     2521 gccatggggg aacgccttcg tgttccgcgc cgccccagag ttccgggacg ggcgcacttt
     2581 tataccatcg tgtctggatg aaggccaaag ctcaccgcga tggccgtgtc tacctgcttc
     2641 atcatcgttt cgtccacccg gcccatgtgc tcccgcaggc ggcgcttatc cagggtgcgg
     2701 atctgctcca gcaggaccac cgagtccttg ggcaggccgc agctcatggc ggccagggag
     2761 atgtgggtgg gcaggcgcgc cttgccggtc tgggaggtga tggcggcggc aatgacagta
     2821 gggctgtgcc ggtttccggt gtcgttctgc acgatcagca ccgggcgcac gcccccctgt
     2881 tcgctgccca ccaccgggct caggtcggca tagaaaatat cgcctcgttt gactacactg
     2941 ttgtccacgt gatttcacac tccgccggaa gataatacgg cgttacagcg gaccggcagc
     3001 ggtctttatg taacgctgta ttatgatttt cccacggggc ggcaaggttt atacatgcag
     3061 ggaaaaaact ccgcagcccc tgcccggcgg cgggcggcgg cccgctgctc cataccatct
     3121 tatgcggggc agctgggatc ggtccctgtc actccccccg gcccgtatag atccggggga
     3181 ccctggggtt cacgttgcac agcagctcat aggggatggt gcccgccagc gcggcggcct
     3241 gctccagcag accctcccgg ccatagagct ccgccacgtc cccggggacc gcgtccggga
     3301 catctgtcac gtccaccatg cacatgtcca tgcagatacg gcccaccacg gggcagcgcc
     3361 ggccccgcag cagcacctcc atccggttgg agagcaggcg gggatatccg tccccatagc
     3421 ccaccggcag cacggccagg cggctgtccc gctccagccg ggcagtgcag ccgtagctga
     3481 cacaggtgcc cgcaggaacg gcccgcacgg cggccacccg agtcttcagc gccatcaccg
     3541 gcagcaggcc ggagctgtcc agtccctcca gtcccggcgc gggataatag ccgtacaggg
     3601 cgatgccggg acggaccata tccatatgag tacaaggata gtttaacacg gccgcgctgg
     3661 cagcacaatg gcaaattttg aaccggaccc ccagcgcttc cagagcctgc ctggcatcca
     3721 ggaaacgggt gaactgcccc atggtatact cctcgttccc gtcggcgtcc gcaaaatggg
     3781 taaacagccc ctccgcctcc aggccgggca gggcgcacag cgccgcaatg gcggcggcgg
     3841 tctcccgctc acccccctcc cgccagagaa agcccagccg ggtcatgccg gtgtccacct
     3901 tcacgtggat cttcagcgtc cgtcccgcag cctgtgccgc gcgggccagg gcccggccgg
     3961 tctccaggtc ggagacagtc tgggtcacat catgctccag cagcagctcg gccagctggg
     4021 gcggcgtctg ccccaggcac aggatgggga tggtcacgcc gccgcggcgc agctccgccg
     4081 cctcatccac acaggccacc gccagcatat cggcccccag cgcctccagc ttccgcccca
     4141 cctggagggc cccgtggccg taggcgttag ccttgcacag ccccaaaaag cggcagtccg
     4201 gagccagcat ggcccgcaga gtctggtagt tatgggccag acggtccagg gatatctccg
     4261 cccaggtcct catttgcgcg gtctctgtca tggtaaaacc tctattcctt ggtaaattga
     4321 gaaaattcgc attggagcac acacacccca tcctgggaga tctctccccg gaccagggcg
     4381 tgggtggcgg cgtcgaacca cagggtggtc tccgtcccgc tgccctgttt ctcctccggg
     4441 tcccggctca ccacccgcag ggcctggact tcccccagct cctccaggac acaggtgtcc
     4501 agatagccgg accgtgcggt ctccagcagg gcggggatgg cccccatggg ggtcagtccg
     4561 ccgggagcca gctctccggt ctccaggatg gctccatcgt actccagcca gccctcatcc
     4621 tcttcgatcc gggccgtcag gccggccacc gtctccggcg cggtcagggt gaagagggct
     4681 tcctccccgt tcaccgcagc cgacagctca taccggtaga cccgctggcc ataatccgct
     4741 gtcaccacgg cctgggccgc gcaggacgtc atagccagat actccccccg gatggtgagg
     4801 gcgagctcct ccgcctcgct gcctcccccg cctccgccgc aggcggagag ggagagggtc
     4861 atcatcagta cgcaaatcag cagggatctc cgcaccgctc cgacctccaa attcaggatc
     4921 cggaccggac gtgggcctcc agggtctcag gcagccgctc caccaggtcc ccgggcgtca
     4981 taccgtactc ccccaggctg ccggcgcagc ggtccccggc ctccccgtgg agatatacgc
     5041 cgtcccgggc cgccagctcc ggctcccacc cctgtcccag cagggcgagg atgagtccgg
     5101 ccagcacgtc gccgctgccc cccttggaca tgcctggatt gcccgtggtg ttgatccagg
     5161 cccggccgtc cggggcagcg gtgacggtgc ggtgcccctt cagcaccagc acacaccggt
     5221 ggtcccgggc aaagtcccgg gccgccgtca gggggtcctc cagcggcagg ggccgcccgg
     5281 tcagccgggc gaactctccg gcgtgggggg tcaggatggt gggagccctt cgtccgtcca
     5341 gactatctat atgcccgtcc agcgcgttta tgccgtcggc gtccagcacc accggcacct
     5401 tcaggtcctc cagcagggaa cggaccagcc gctccgtccg gggcgcccgg cccagtcccg
     5461 ggccgatgag caccccttcg caggacgacg cccgcctcag gatatcctca taccgcgccg
     5521 gcagggggaa gggcatggcc tccagacact tcaccgccgc gatgggccag atctcctccg
     5581 gcacgcccag ggacaccagg cccgccccgc cgcgcaccgc gccggaggcg gccagcaccg
     5641 gggccccggt atagcccacg ctccctgcca ggatcagcac ccgcccgtag tcccccttgt
     5701 gggagtcccg ctttctccgg ggcaggacca catcctcata tcccatcacc cccaccggga
     5761 agagcatctg tgctccgggg ccgaagatga gcctgtgggg gatgccgatg tccgctactg
     5821 tgaccagtcc ggcacaagag gccccctccc ccagatagag gccgggcttt gcgcaggtga
     5881 acgtgacggt ccggtccgcg cggacggcct cacccaatac ggcgccagtg tcggcgtgga
     5941 ccccggaggg gatgtcacag gacaccaccc gcttcagcgg ggaccttccc tgcatcaatc
     6001 gcacagccag aagggcgtcc ccctccacag ggcgggagag ccccaccccg aacagggcgt
     6061 ccaccaggca gccgcacaga gacaaccaca gcatctgccg gcggtcctcc ggccggaact
     6121 cctccagcac gcctccagct tcttccagct ttttctccat ggcccgctcg tccggcgtca
     6181 tcttcctccg gtcccccacc aggaaggccc ggacccgaaa gcccatgccc cggaggatgc
     6241 gggcggcggc cacgccgtcc ccgccgttgt tgccggggcc gcacagcacc cccacccagg
     6301 gagcgtgatt ctgatttttc tcctccgcaa gggcctgaag ggcctccagc tcccgccggt
     6361 cctcctccgt ctccgcccct gctcccagga ccgtcacaga gtcggctccg ccgatgaagt
     6421 ccgggccgcc gccctccagc agcccccgca cctcctccgc
//
LOCUS       DBMDSJ010000046         6315 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3583_length_6315_cov_1.746379, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000046 DBMDSJ010000000
VERSION     DBMDSJ010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3583_length_6315_cov_1.746379"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="ACZPAQ_01985"
     CDS             <1..859
                     /locus_tag="ACZPAQ_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR0902778.1"
                     /translation="FNLTSLRKAFGPEEGKRLGEKLIAAYGAEKKVTILELRQNQDPE
                     LAKVADYVYEHVFVHYTMKQWGQTPDQIDPNTTARVPVFLSEDDRYFQDEYQGMPLKG
                     YAPLFEAMLDHSNITVRLGEDAIPRLGLDGDVLTVDGVPFQGPVVYTGAADELFGRRF
                     GPLPYRTLDFRFETLDQTWFQSRGTVNYTVDQPYTRITEFKHMTGQELEGRTTIVKEF
                     SRAYTGAPEETPYYAIINGENNALYARYRALAERFPNLHLLGRLAEYKYYNMDAIVAR
                     ALELAGQLI"
     gene            909..1937
                     /locus_tag="ACZPAQ_01990"
     CDS             909..1937
                     /locus_tag="ACZPAQ_01990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0902779.1"
                     /translation="MDKLITFAVPCYNSAAYMDHCVETLLQGGDDIEIILVDDGSTKD
                     DTPAICDRYAQQYPDIVRAIHQENGGHGEGVNQGIRNARGVYYKVVDSDDWVDVPALK
                     KALDKLRQFVRDGKLVDMLVCNYVYEHVEDNTQRVMHYRNVFPRNRVFGWEQIGRFRP
                     SQYLLMHSVIYRTQLLRDCGLELPKHTFYVDNIFVYQPLPSVRNIYYLDVDLYRYFIG
                     RADQSVNETVMVGRVDQQLRVTYQMIDSHDLRVLTAEQPKLGRYMFNYLAMMMAISSI
                     FLTIADTPECLGKKTQLWEYLRTVDTGIYHKMKYRAVSAFTNFPGYQGRRLSVGLYRL
                     ARKIYKFN"
     gene            2093..3205
                     /locus_tag="ACZPAQ_01995"
     CDS             2093..3205
                     /locus_tag="ACZPAQ_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR0902780.1"
                     /translation="MPTKRLACLLTCLLLVLTLPACGGKGAASEPEGPNEPAVSVLVP
                     QPEPEPEPVLPYTNPLTGEGCEVDVGARRPIAVMLNNLKKALPQVGVSQADVIYEILA
                     EGGITRMMAVFQDVAGAGEIGTVRSARDYYASLAYGHDAVYLHAGGSPQAYEFIKSRG
                     VTALDCVNGPYEGTLFWRDQERRRSAGLEHSVLTSSETIQELLPTYQRVRLDHEEGFQ
                     VGWTFQEGPEAGGEAAEILTVPFSTYKTGVFEYDPDSGLYLVEEYGEPYVDGNTGEQV
                     AVKNVLVLYTDVSQLSGDSAGRLKVRTTGSGGGLLVRDGLAWPITWEREGESDRLSFY
                     REDGQPAALGVGHSYINIVKTGTEVTWAAKSPEEAG"
     gene            3639..5009
                     /gene="mgtE"
                     /locus_tag="ACZPAQ_02000"
     CDS             3639..5009
                     /gene="mgtE"
                     /locus_tag="ACZPAQ_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR0902781.1"
                     /translation="MHENYDLDLLLELVEQRKFRSMKELLSQMNEVDIAEFLDELDVE
                     QEILVFRLLPKDTAAEVFTYLENTEDQEKLIGALSDRELREVLDELYLDDTVDIIEDM
                     PANVVSRILRNTDASTRSQINQLLNYPKDSAGSIMTTEFVFLHPDATVEESFARIRSV
                     GIDKETIYTCYVTQNRVLLGVVTVRRMLLSSYETRIRDIMETNVLSVNTHEDKEDVAQ
                     LLNKYDLSAIPVVDGDNRLVGIVTFDDAMDVIEEETTEDFEKMAAILPSDKPYLKTGV
                     LETWRSRIPWLMLLMLSATFTGIIITSFESALAACVVLTSFIPMLSGTGGNSGSQSSV
                     AVIRALSLDEIDFSDLVAVVWKEIRVAVLCGVCLAAANFVKMMVVDRWLMHNPEVTPL
                     VALVVCLTLVFTVLCAKVVGCTLPMAAEKLGIDPAVMASPFITTIVDALSLLIYFFFA
                     TWILPL"
     gene            5147..6139
                     /locus_tag="ACZPAQ_02005"
     CDS             5147..6139
                     /locus_tag="ACZPAQ_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0902782.1"
                     /translation="MYQWERQIQSIVDEIDRCIRQQDSEALTLQALSRRLGYSESYTT
                     RKFGEISGMRLRDYLRGRRLAFALKEVRDSEKSLLDIALAYGFSSQEAFTRGFRRAYG
                     VPPGEYRRNPRPVVLRTKLRPIDRYFFGTEEIGMIGSCQGVKIYFVTIPAHKFLYLED
                     RESNGYWDFWQRQAEIPGQDYDTVCGLLDSIKGKLDDSGGSEANCGSGQIMAYRNDPK
                     GRLCGWGIPRTECWGVRLPSDYGGGVPPQLRLMDVPEGEYVVFEHGPFDYEGENRVVE
                     ERIEAAMAGFDYTGAGCRLDLTPGRLMYFYYDPGRCFKYVRPVERLEGGASGQG"
BASE COUNT         1226 a         1904 c         2093 g         1092 t
ORIGIN      
        1 cttcaacctg acctccctgc ggaaggcttt cggacccgag gaggggaagc gtctggggga
       61 gaagctgatc gccgcctacg gcgcagagaa gaaggtcacc atcctggagc tgcgccagaa
      121 tcaggacccg gagctggcca aggtggccga ctacgtctat gagcacgtgt tcgtccacta
      181 caccatgaag cagtggggcc agaccccgga tcagatcgac cccaacacta ctgcccgggt
      241 gcctgtcttc ctgtcggagg acgaccgcta tttccaggac gagtatcagg gcatgcccct
      301 gaagggctac gcccccctct ttgaggccat gctggaccac tccaacatta ctgtccggct
      361 gggggaggac gccatccccc gcctgggact ggacggggac gtgctgacgg tggacggcgt
      421 cccgttccag ggaccggtgg tctacaccgg cgcggcggat gagctgttcg gccgccgctt
      481 cggccccctg ccctaccgca ccctggactt ccggttcgag accctggacc agacctggtt
      541 ccagagccgg ggcacagtga actacaccgt ggaccagccc tacacccgca tcaccgaatt
      601 caagcacatg acgggccagg agctggaggg caggaccact atcgtgaagg agttctcccg
      661 ggcctacacc ggcgcgccgg aggagacgcc ctattacgcc atcatcaatg gggagaacaa
      721 cgcactgtat gcccgatatc gggcgctggc agaacggttc cccaacctgc acctgctggg
      781 ccgcctggcg gaatataaat actacaacat ggacgccatc gtggcccggg cgctggagct
      841 ggccggacag ctgatctgac cctgcggccc ggaccgcgcc caaaacctga cagacaggag
      901 aaagaagtat ggataagctg atcacctttg ccgtcccctg ctacaactcg gcggcctata
      961 tggaccactg tgtggagacg ctgctccagg ggggcgacga catcgagatc attctggtgg
     1021 acgacggctc caccaaagac gacacccccg ccatctgcga ccgctatgcc cagcagtacc
     1081 ccgatatcgt ccgggccatc caccaagaga acggcggcca cggcgaggga gtcaatcagg
     1141 gtatccggaa cgcccggggc gtctactaca aggtggtgga ctccgatgac tgggtggacg
     1201 tgcccgctct gaagaaggcc ctggacaagc tgcgtcagtt cgtgcgggac ggcaagctgg
     1261 tggatatgct ggtgtgcaac tacgtctatg agcatgtgga ggacaacacc cagcgggtga
     1321 tgcactaccg caatgtgttt ccccgcaacc gggtattcgg ctgggagcag atcgggcgct
     1381 tccgcccctc ccagtatctg ctgatgcatt cggtgatcta ccgcacccag ctgctgcggg
     1441 actgcgggct ggagctgccc aagcacacct tctatgtgga caacatcttc gtgtatcagc
     1501 ccctgccctc ggtgcggaac atctactatc tggatgtgga cctgtaccgc tacttcatcg
     1561 gccgggcgga ccagtccgtc aacgagacgg tgatggtggg ccgggtggac cagcagctgc
     1621 gggtcaccta tcagatgatc gactcccacg acctgcgggt cctcactgca gagcagccca
     1681 agctggggcg gtacatgttc aattatctgg ccatgatgat ggccatctcc tccatcttcc
     1741 tcaccatcgc cgacaccccc gagtgcctgg ggaagaagac ccagctgtgg gaatatctgc
     1801 ggacggtgga caccgggatc tatcacaaga tgaagtaccg ggccgtctcc gcctttacca
     1861 atttcccggg ttatcagggc cggcggctgt cggtgggcct ctaccggctg gctcggaaga
     1921 tctataagtt caactgaacg cccagtttga cagcgggccg cccttttggc ggcccgctgt
     1981 gtattttggg ccccgccggc aagttttccc cctccatcga cacgcatgag attgacaccc
     2041 gcccctgggg cgggtatgat aaaggaaaga aagcaagagg gggagatctg agatgcccac
     2101 caaacgcctg gcctgcctgc tgacctgcct gctcctggtc ctgacgctgc ccgcctgcgg
     2161 cgggaagggg gctgccagcg agcccgaggg ccccaacgag cccgcggtgt cggtcctggt
     2221 gccccagccg gagcctgaac cggagcccgt cctgccctac accaaccccc tcaccggcga
     2281 gggctgcgag gtggatgtgg gcgcccggcg gcccatcgct gtcatgctca ataacctgaa
     2341 gaaggcactg ccccaggtgg gggtatccca ggcggatgtg atctatgaga tcctggcgga
     2401 ggggggcatc acccgaatga tggccgtgtt ccaggatgtg gcgggggcgg gagagatcgg
     2461 aacggtccgc tccgcccggg actactatgc ctccctggcc tatggacacg atgcggtcta
     2521 cctccacgcc gggggcagcc cccaggcata tgagttcatc aagagccggg gcgtcactgc
     2581 cctggactgc gtcaacggcc cctatgaggg caccctgttc tggcgggacc aggagcgtcg
     2641 gcggagcgcc ggcctggagc actcagtgct cacctccagc gagaccatcc aggagctgct
     2701 gcccacctac cagcgggtgc gtctggacca cgaggagggg ttccaggtgg gctggacctt
     2761 ccaggagggt ccggaggccg gaggagaggc ggcggagatc ctcaccgtcc ccttctccac
     2821 ctataagacc ggcgtgtttg aatacgaccc ggacagcggg ctgtatctgg tggaggagta
     2881 cggcgagccc tatgtggacg ggaacaccgg agagcaggtg gcggtgaaaa acgtgctggt
     2941 gctctatacc gatgtgtccc agctctcagg ggacagcgcg ggccggctga aggtgcgcac
     3001 taccggcagc ggcggcggac tgctggtgcg ggacggcctg gcctggccca tcacttggga
     3061 gcgggaggga gagagcgacc ggctctcctt ctatcgggag gacggccagc cagcggctct
     3121 gggcgtgggc cacagctaca tcaacatcgt caagaccggc acggaggtga cttgggcggc
     3181 aaaaagcccg gaggaggccg gatgagcaaa gattttactt gaaaaaatgc ggatgggaat
     3241 gctatactga gagatagata caggtcaccg cccggagcgg acggggaccc atacaggcag
     3301 aagggggagc cccctgagca tatgccggga gaggatgtgg gaacgcatgg aaggccgaag
     3361 tcgccatcgg gatcgggacg accgctgtca cgaacggggg gcggcgcaga acaggggcct
     3421 gctgcggtcc cgccgcgccg ccggtatctg aacagatcgg gggatgggaa gcccccgccc
     3481 ctttcgtgtg cggccacagg gtcgaagtgc atctgaaaga cggcaggagc cgaaagcggg
     3541 gctccgccca gcctgccgtt ttccagatgt ccgtcgccct tgtggccttt ttgcgtcccg
     3601 aattgttcca agcgatgaac aaaaggggga aataacgcat gcacgaaaac tatgacctgg
     3661 acctgctgct ggagctggtg gaacagcgga agttccgcag catgaaggag ctgctgtccc
     3721 agatgaacga ggtggacatc gccgagttcc tggacgagct ggacgtggag caggagatcc
     3781 tggtcttccg cctgctgccc aaggacacgg cggcggaggt gttcacctac ctggagaaca
     3841 ccgaggacca ggagaagctg atcggggccc tcagcgacag ggagctgcgg gaggtgctgg
     3901 acgagctgta cctggatgac acggtggaca tcatcgagga catgcctgcc aacgtggtct
     3961 ccaggatcct gcgcaacacc gacgcctcca cccgcagcca gatcaaccaa ctgctgaact
     4021 atcccaagga ctccgccggc tccatcatga ccacggagtt cgtctttctc cacccagacg
     4081 ccacggtgga ggagtccttt gcccgcatcc gcagcgtggg catcgacaag gagaccatct
     4141 acacctgcta tgtcacccag aaccgggtgc tgctgggggt ggtcacggtg cggcggatgc
     4201 tgctgtcctc ctatgagacc cgcatccggg acatcatgga gactaacgtc ctctcggtca
     4261 acacccatga ggacaaggag gacgtggccc agctgctgaa caagtacgac ctgtccgcca
     4321 tcccggtggt ggacggcgac aaccggctgg tgggtatcgt cacctttgac gacgccatgg
     4381 acgtcattga ggaggagacc accgaggact tcgagaagat ggcggccatc ctgccctcag
     4441 ataagcccta cctgaagacc ggcgtgctgg agacctggcg ctcccgcatc ccctggctga
     4501 tgctgctgat gctctccgcc accttcaccg gcatcatcat cactagcttt gagagcgccc
     4561 tggcggcctg tgtggtgctc acctccttca tccccatgct ctcgggcacc ggcgggaact
     4621 ccggctccca gtcgtcggtg gcggtgatcc gcgccctgtc cctggatgag atcgacttct
     4681 ccgacctggt ggccgtggtg tggaaagaga tccgtgtggc ggtgctgtgc ggcgtgtgcc
     4741 tggctgccgc caacttcgtc aagatgatgg tggtagaccg gtggctgatg cacaacccgg
     4801 aggtcacccc cctggtggcc ctggtggtct gcctgaccct ggtctttacc gtgctgtgcg
     4861 ccaaggtagt gggctgcact ctgcccatgg cggcggagaa gctgggcatc gacccggcgg
     4921 tgatggcttc ccccttcatc accaccattg tggacgcact ctccctgctg atctatttct
     4981 tcttcgccac ctggatcctg cccctgtaag tgcgctccct gccggccttc tgaccggcgg
     5041 ggatttttat atctgggaag gggaactgag cagctgggat caagcggcgg cggaggaaat
     5101 ggggtatcat aagggggaag acacattaat ggaaggagcg gtggaaatgt atcagtggga
     5161 gcggcagatc cagagcatcg tggacgagat agaccggtgt atccggcagc aggacagcga
     5221 ggcgctgacc ctgcaggccc tgtcccgcag gctgggctac tcagaatctt ataccaccag
     5281 aaagttcggg gagatctcgg gcatgcggct tcgggattat ctgcggggca ggaggctggc
     5341 ctttgccctg aaggaggtgc gggacagcga gaaaagcctg ctggacattg cgctggcgta
     5401 tggcttttcc tcccaagagg cgttcacccg gggcttccgg cgagcctatg gcgtgccccc
     5461 gggggagtac cggcgcaacc ccaggcccgt ggtgctgcgt accaagctgc gccccattga
     5521 ccggtacttt ttcggtacgg aggagatagg catgatcggc tcctgccagg gggtgaagat
     5581 ctactttgtc accatccctg cccacaaatt cctgtatctg gaggaccggg agagcaacgg
     5641 ctactgggac ttctggcaga gacaggccga gatcccgggg caggactacg acaccgtctg
     5701 cggcctgctg gacagcatca agggaaagct ggacgacagc ggcgggagcg aggccaactg
     5761 cgggagcggc cagatcatgg cctaccggaa cgacccgaaa ggccgtctgt gcggctgggg
     5821 gatcccccgg accgagtgct ggggggtgag gctcccctcg gattacgggg gcggggtgcc
     5881 gccccagctg cggctgatgg acgtgccgga gggggagtac gtggtgttcg agcatgggcc
     5941 ctttgactac gagggagaga accgtgtcgt ggaagagcgg atcgaggccg ccatggccgg
     6001 cttcgactac accggggctg gctgccgcct ggatctcacc cccgggcggc tgatgtattt
     6061 ttactatgac cccgggcggt gcttcaaata cgtcaggccg gtggagcgcc tggagggcgg
     6121 ggcgtccgga caggggtgag gagagcggaa cagggacggg gggccgggag accgggcccc
     6181 cgtttttttg cccccggcag gggaggccgg aactttttgg gagcgcggac gtctaaagga
     6241 gcagagacag caggcagaaa attttccgtt ccgtgcagca aaagggcggg ctcatccgtc
     6301 tgaagggata gaaag
//
LOCUS       DBMDSJ010000047         6294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3597_length_6294_cov_1.927822, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000047 DBMDSJ010000000
VERSION     DBMDSJ010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3597_length_6294_cov_1.927822"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="ACZPAQ_02010"
     CDS             complement(<1..1422)
                     /locus_tag="ACZPAQ_02010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902783.1"
                     /translation="MKQISWYRSIRFRIPAVIALVLLVPIVVFWHHSMELTRKNMLSQ
                     TSNLIYTNLYGASLLMDDVMEQVSDFSREISQDPGFLSLISQYQQADAGSDQRQKARS
                     QLSLALSQYVGQLRTLDSIYLMFPETETVVTMLPGQKELSFSHHYAQQLYQMYYDVFE
                     SPIVWHLLPSAGGGSRMLSHIRPVSGPAQQEKCILICNLKDSAWQTALSALELYSGSV
                     WISNYAGQVLLSYGSTLSTSQNIGQDSRFSQAFAQNRQYGTYSYQEDGTDYLISYYNA
                     VESGWKYLSCVPEDIVLSGSGGARAFSLLALLCGALGISLGSLILYRYVVRPISALMS
                     HLSDMEQGTLAQAPVPVQRDEISLLFHRYNQMIQRLQKLIDEIYVQQLLRKQAELSFL
                     QSQMDEHFLYNTLNTIYSQASHEHADTSARMILTLSRYFRISLSQGSDKLPLDQLADL
                     LRSYLQLQQMRFGRSLVFQIQRFP"
     gene            complement(1654..2967)
                     /locus_tag="ACZPAQ_02015"
     CDS             complement(1654..2967)
                     /locus_tag="ACZPAQ_02015"
                     /EC_number="4.4.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0080146 - L-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cysteine desulfidase family protein"
                     /protein_id="HIR0902784.1"
                     /translation="MDQALYQNHLGILREELLVALGCTEPIAIAYAGAKARQLLGVMP
                     EHCRVRCSGNIIKNVKGVIVPNSGGMRGIDVAATLGIVGGDPDRELAVLETVTPADIQ
                     TTQALVKEGFCTCELVEDVDNLYIVVELEGGGHSAEIEIQEHHNNITYMKKDGTVLLD
                     SRPDPSCKKQSGGPDRMLLNVANILEFADTVKMEDVEELIGRQIDYNTAISDEGLKGN
                     YGVETGRILMENAGNASGLNRVRLQARAAAAAGSDARMSGCSLPVVINSGSGNQGITV
                     TMPIVIYGRAWQADRELILRALVLANLISVHQKKYIGSLSAYCGATSAATGAACGVAY
                     MMLRQKGEQERIYQILCDTITNSIETLGGMVCDGAKASCAAKIGLAVENALMALELSL
                     AGNVFQPGEGMTMEDPEDTIAAVGRMGRVGMKSTDVEILNIMLGN"
     gene            3081..4085
                     /locus_tag="ACZPAQ_02020"
     CDS             3081..4085
                     /locus_tag="ACZPAQ_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0902785.1"
                     /translation="MFSLTSLKCFAAAAEELNFTRAAKRLFISQQALSSHISKLEEYY
                     GVRLFDRGIPMTLTDAGRALQKHAREILSSVDDYAREVQDIKNFRQGELTVGIPVTRG
                     TIMLPPLLSAFHQLFPQIRLHLVEGTVSGNIADALYDGTADLCIGYQPENTEELAVTP
                     LFEEKFVVLVPDRLMKEHHLDRKLGEGALSMDRFAELPFVVQHPETMNGRVFQTLCRN
                     AGIEPEVVVSTQNLITEASLCMEGMGACVLPLTFLSPDQRLSGHGSTPLFSQEGLDRL
                     SIFLLDTSGIGRFQISVCRLKSKLLTRAGREFISLAREIYSGVSAGQIPPQFHFESSR
                     "
     gene            complement(4036..5040)
                     /locus_tag="ACZPAQ_02025"
     CDS             complement(4036..5040)
                     /locus_tag="ACZPAQ_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR0902786.1"
                     /translation="MTKKERIQAAIRGEKPDKMPYSFWTHMPGTDLDPEAISQKTYEF
                     YKKYDFDFVKTMNNGMYAIEDFGCTVDYSEIVKGGVAKVVSTPISCAADWRKLTVCPC
                     TQGSNARELRHLQLVLEKLKGEEVPVIFTAFTPITIADKISGGKLISYIEEGHGDDVK
                     AALEVITETTAQLVAAAIDMGADGIFLASQMSSYDKCTDRQYLEYGKPYDLRVLEAAG
                     KGWMNTIHCHGDHIMFHILKDYPVQVFNWHAWESLPALDEAYALTGKCLMGGLSRTDI
                     TQQNRGAIQNQIFQCFKLLGGRHQILTPGCVIRYPLDDDMLSFIGTTRNEIEAVFDQR
                     "
     gene            complement(5125..>6294)
                     /locus_tag="ACZPAQ_02030"
     CDS             complement(5125..>6294)
                     /locus_tag="ACZPAQ_02030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR0902787.1"
                     /translation="GYVKERIRKMQDVMARKGVDVTILMRPENVFYFSNFNPILISHV
                     PYFILTRDDAFLLVHCIRHDHAVNEGAVDKVLCYGKWGATQAVAMDAMDAMRELLGGG
                     PLTVGVEGDYASMNFLDRLKTKLQVKGYVDIAQDAVDLRLIKDPHEIKMCRISGELVD
                     LGVTRAIEALEGGASEAQAATEGQYAMRNRWHEKYQQYEVSGFSNSETADIDTFVVWC
                     MSNERLNYGCDCPTGYVPMPGDLTMPMSWARIAGYNVENERTVIVGHVNEERTRAYDA
                     MLRARDNIFRMLRPGAIFEDLYFAAMKEYEDAGFGSILPGRCGHGMGLSTHEFPSVTK
                     GNKAPLAPGMILTVEPGLMSAELGAVRNSDTVLITEDGFEFLTNSPRDKIIIKKG"
BASE COUNT         1143 a         1831 c         2063 g         1257 t
ORIGIN      
        1 cgggaaacgc tggatctgaa agacaagcga gcggccgaag cgcatctgct ggagctggag
       61 atagctgcgg agcaggtcgg ccagctggtc cagagggagc ttgtcgctgc cctgagagag
      121 actgatccgg aaatatcggg acaaggtcag gatcatccgg gcagaggtgt ccgcgtgttc
      181 atggctggcc tggctgtaaa tggtgttcag agtattgtac aggaagtgct cgtccatctg
      241 ggactggaga aacgaaagct cagcctgctt tcggagcagc tgctggacat agatctcgtc
      301 gatgagcttt tgcagccgtt ggatcatttg gttgtaccgg tggaagagca ggctgatctc
      361 atcccgctgg accgggactg gagcctgggc gagtgtgccc tgctccatat cgctcagatg
      421 gctcataagg gcggaaatgg gcctcaccac ataccggtac aggatcagac tgcccagaga
      481 gatgcccagg gcaccgcaca gcagggcaag gagagagaag gcccgagcgc cgccggagcc
      541 ggagaggacg atatcttccg ggacacagga gagatatttc catccgctct ctacggcgtt
      601 atagtaggag atcagatagt cggtgccatc ctcctggtag gagtaagtcc catactggcg
      661 gttttgcgca aaagcctggg aaaagcggct gtcttggcca atgttctggg acgtggaaag
      721 cgtgctccca taggagagga gcacctgtcc tgcataattg ctgatccaga cagaaccgga
      781 gtacagctcc agagcggaaa gagcggtctg ccaggcggag tccttcagat tgcagatcag
      841 gatgcacttt tcctgctggg caggacctga gaccggccgg atatgggaga gcatccggct
      901 gcctccgccg gcggagggga gcaggtgcca gacgatgggg gactcaaaaa catcgtagta
      961 catctggtac agctgctgag catagtggtg ggaaaaggag agctccttct gcccgggcag
     1021 catggtgacc actgtctctg tctcggggaa catgagatag atggaatcca gggtacgcag
     1081 ctggcccaca tactggctca gagccaggga gagctggctg cgtgcttttt ggcgctggtc
     1141 agaaccggcg tcggcctgct ggtattggga gatcagagag agaaagccgg ggtcctgact
     1201 gatctcccgg ctgaaatcac tgacctgttc catcacatca tccatcagga gagaggcccc
     1261 gtagaggttg gtatagatca gatttgatgt ttgactcagc atattttttc gggtcagctc
     1321 catggaatgg tgccagaaga ccacgatggg gaccagaagg acgagagcga tgacagcagg
     1381 gatgcggaag cggatgctgc ggtaccagga aatctgtttc ataggcttcc tccagcaata
     1441 gagtggtctt gtttgtgttc tattataggt attttccggc gcaccggcaa gagaaatcgt
     1501 accggcgaga gaaaagggcc ggacggcgaa agccgcccgg cctcaggctg ttgaaagagt
     1561 ccccgctaag gtgggggtgg gagaaagcgg ggagaataag accgcggccg gacaggatgc
     1621 tcccgtccgg ccgcgtatcg tctggggaca gcttcagttc cccagcatga tgttcaggat
     1681 ctctacgtcg gtgctcttca tacccacccg gcccatgcgg cccactgccg cgatggtgtc
     1741 ctccgggtcc tccatggtca tgccctcgcc gggctggaag acgttgcctg ccaggctcag
     1801 ctccagggcc atcagggcgt tctccaccgc caggccgatc ttggccgcac agctggcctt
     1861 ggccccgtcg cacaccatgc cgcccagggt ctcgatgctg ttggtgatgg tgtcgcagag
     1921 gatctgatag atccgctcct gctccccctt ctggcggagc atcatatagg ccacgccgca
     1981 ggcggccccg gtggcggcgc tggtggcgcc gcagtaggcg ctgaggctgc cgatgtactt
     2041 cttctgatgc acgctgatca gattggccag caccagggcg cggaggatca gctcccggtc
     2101 cgcctgccag gcccgcccat agatgacgat gggcatggtc acagtgatgc cctggttgcc
     2161 gctgccgctg ttgatgacta ccggcaggga gcatccgctc atccgggcgt cgctgcctgc
     2221 ggcggcggcg gcccgggcct ggaggcggac acggttcagc ccgctggcat tccccgcgtt
     2281 ctccataagg atgcggccgg tctccacacc gtagttgccc ttgagaccct cgtcagagat
     2341 ggcggtgtta tagtcgatct ggcggccgat gagctcctcc acatcctcca ttttcaccgt
     2401 gtccgcgaat tccaggatgt tggccacatt gaggagcatc cggtccggac cgccgctctg
     2461 ttttttgcag ctggggtcgg ggcggctgtc cagcaggacc gtgccgtcct tcttcatgta
     2521 ggtgatgttg ttgtggtgct cctggatctc gatctcggcg gagtgtccgc cgccctccag
     2581 ctccaccacg atatacaggt tgtccacgtc ctccaccagc tcgcaggtgc agaagccctc
     2641 cttcaccagt gcctgggtgg tctggatatc cgccggcgtc acggtctcca gcacggccag
     2701 ctcccggtcc gggtctccgc ccacgatgcc cagggtggcc gccacgtcga tgccgcgcat
     2761 accgccggag ttggggacga tgacgccctt cacgtttttg atgatgttgc cgctgcaccg
     2821 gacccggcag tgctctggca ttacgcccag cagctgccgc gccttggcgc cggcataggc
     2881 gatggcgatg ggctcggtgc agcccagggc caccagcaat tcctcccgaa ggatgccgag
     2941 gtgattctga taaagtgctt gatccatgct gtatcgctct cctatctggg gtggtatctg
     3001 aaaatgaaca cttgtaaagc acaagcaaat cgaataaaat aagcaagaga tagacaactg
     3061 tttgattgga ggagacggcc atgttcagtc tgaccagtct gaaatgcttt gcggcggcgg
     3121 cggaggagct gaacttcacc cgggcagcca agcgcctgtt catctcgcag caggccctga
     3181 gcagccacat ctccaagctg gaggaatatt acggggtcag gctgtttgac cggggcatcc
     3241 ccatgaccct gaccgatgcg ggacgggccc tgcagaagca tgcccgggag atcctgagct
     3301 cggtggacga ctacgcccgg gaggtccagg acatcaagaa cttccggcag ggcgagctga
     3361 ccgtgggcat cccggtgacc cgggggacca tcatgctgcc gcccctgctg tccgcctttc
     3421 atcagctctt cccccagatc cggctccatc tggtggaggg gaccgtcagc gggaacatcg
     3481 cggatgcgct gtacgatggc acagccgacc tgtgcatcgg ctatcagccc gaaaacacgg
     3541 aggagctggc ggtgacgccg ctgtttgagg aaaagttcgt ggtgctggtc cctgaccggc
     3601 tgatgaagga gcaccatctg gaccggaagc tgggggaggg cgccctgtcc atggaccgct
     3661 ttgcggagct gccctttgtg gtgcagcatc cggagaccat gaacggccgg gtctttcaga
     3721 ccctgtgccg gaacgccggg atagagccgg aggtggtggt ctccacccag aatctgatca
     3781 cggaggcatc cctgtgcatg gagggaatgg gggcctgtgt gctgcctctg accttcctgt
     3841 cacccgacca gcggctctcc ggccacggga gcacccccct gttcagccag gaggggctgg
     3901 acaggctctc tattttcctg ctggatacca gcgggatcgg ccggttccag atctcagtct
     3961 gccgcctgaa gagcaagctg ctcacccggg cgggccggga attcatcagc ctggcccggg
     4021 aaatttacag cggcgtcagc gctggtcaaa taccgcctca atttcatttc gagtcgtccc
     4081 gataaaggag agcatgtcgt cgtccagggg gtagcggatg acgcagcccg gggtgaggat
     4141 ctgatgccgt ccgcccagga gcttgaagca ctggaagatc tggttctgga tggcgccccg
     4201 gttctgctgg gtgatgtcgg tccggctcag gccgcccatg aggcacttgc cggtgagggc
     4261 ataggcctcg tccagggcgg gcagggattc ccaggcgtgc cagttgaaga cctggacggg
     4321 gtaatccttg aggatgtgga acatgatgtg gtctccgtgg cagtggatgg tgttcatcca
     4381 gcccttcccg gcggcctcca gcacccgcag gtcgtagggc ttgccgtact ccagatactg
     4441 gcggtccgta cacttgtcat aggagctcat ctgggaggcc aggaagatcc cgtcggcccc
     4501 catgtcgatg gccgcggcca ccagctgggc ggtggtctcg gtgatgacct ccagcgccgc
     4561 cttgacatcg tcgccatggc cctcctcgat gtaggagatc agcttcccgc cgctgatctt
     4621 gtctgcgatg gtgatggggg tgaaggcggt gaagatgacg gggacctcct cgcccttcag
     4681 cttctccagg accagctgca gatggcgcag ctcccgggcg ttgctgccct gggtgcaggg
     4741 gcagaccgtc agcttccgcc aatcggcggc gcaggagatg ggggtgctca ccactttggc
     4801 cacgccgccc ttgacgatct cggagtagtc cacggtgcag ccgaagtcct cgatggcgta
     4861 catgccgttg ttcatggtct tgacgaagtc gaagtcatat tttttgtaga actcataggt
     4921 cttctgggag atggcctccg ggtccagatc ggtgcccggc atgtgggtcc agaaagagta
     4981 gggcatcttg tccggcttct cgccgcggat ggcggcctgg atgcgttctt tttttgtcat
     5041 gagatcacag ctcctttttt atggcgctct cgtgcgcctg cgtcaagctc cggcccgcct
     5101 ccagcagagg cgggccggtc aggttcagcc ttttttgatg atgatcttgt cccgggggga
     5161 gttggtcaaa aactcgaacc cgtcctcggt gatgagcact gtgtcgctgt tgcgcaccgc
     5221 gcccagctct gcgctcatca ggccgggctc caccgtgagg atcatgccgg gagccagggg
     5281 ggccttgttg cccttggtga cggaggggaa ctcgtgggtg gacaggccca tcccatggcc
     5341 gcagcggccg gggaggatgc tgccgaagcc ggcgtcctcg tactccttca tggcggcgaa
     5401 gtacaggtcc tcgaagatgg cgccggggcg gagcatgcgg aagatgttgt cccgggcccg
     5461 gagcatggcg tcataggccc gggtgcgctc ctcgttcaca tggcccacga tgacggtgcg
     5521 ctcgttctcc acgttgtagc ccgcgatccg cgcccagctc atgggcatgg tcaggtcgcc
     5581 gggcatgggg acgtagccgg tggggcagtc acagccgtag ttgagccgct cgttggacat
     5641 gcaccagacc acgaaggtgt cgatgtcggc ggtctccgag ttggagaagc cggacacctc
     5701 gtactgctga tacttctcat gccagcggtt gcgcatggcg tactgtccct cagtggcggc
     5761 ctgagcctcg ctggcgccgc cctccagggc ctcgatggcc cgggtgacac ccaggtccac
     5821 cagctcgccg gagatgcggc acatcttgat ctcgtggggg tccttgatga gccgcaggtc
     5881 cacagcgtcc tgggcgatat ccacatagcc cttgacctgg agcttcgtct tcaggcggtc
     5941 caggaagttc atgctggcgt agtcgccctc cacgcccacg gtgagggggc cgccgcccag
     6001 aagctcccgc atggcgtcca tggcgtccat ggccacggcc tgggtggcgc cccacttgcc
     6061 gtagcacagg accttgtcca ccgcgccctc attgacggcg tggtcgtggc ggatgcagtg
     6121 gaccagcaga aaggcgtcat cccgggtgag gatgaagtag gggacgtggc tgatgaggat
     6181 ggggttgaag ttgctgaagt agaacacgtt ctcggggcgc atgaggatgg tgacatccac
     6241 ccctttgcgg gccatgacgt cctgcatctt ccggatgcgc tccttgacat agcc
//
LOCUS       DBMDSJ010000048         6249 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3629_length_6249_cov_2.096129, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000048 DBMDSJ010000000
VERSION     DBMDSJ010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3629_length_6249_cov_2.096129"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="ACZPAQ_02035"
     CDS             <1..361
                     /locus_tag="ACZPAQ_02035"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR0902788.1"
                     /translation="LVVKDGEPIMAIGSPGSWCIPPAIVQILNAVLLFDMDLQQAINE
                     PRAIFTSYSAPLKVYAEPRLPEETIQALIDAGYEVDSGKDWNTSLGSVGVVLVDPDDG
                     FVYAGGDNRRQYKSLAY"
     gene            490..2316
                     /gene="ggt"
                     /locus_tag="ACZPAQ_02040"
     CDS             490..2316
                     /gene="ggt"
                     /locus_tag="ACZPAQ_02040"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR0902789.1"
                     /translation="MKRNNQSLTTKFSALALVLAMMLSLLAGCGQTTQPAASSTPPAA
                     SSEPAASEPETPEIPETMEGDEFGNGAIGTHGGVSSGSVYASQAGLEILQAGGNAVDA
                     AVATAFAVGVCEPNLSGIGGCGMMNIYLADNHEYEILEYMETVPVAVEPGWYNPETDS
                     ATAKNAAVPGQVAGLLTALEKYGTMTPEEVMAPAIKLAREGFPIEERLANAIMDSFDM
                     ISANEEAAAIYTNDGLPYATGDLFKNEPLADTLEAIAKGGIEEFYTGELAQKMVDGLQ
                     ANGSLMAMEDLATYTVAEREPIRTEYYGYEVVTVPPPSNGGDWLLEMLNIMEEKDISQ
                     YDPSTLEYQYIFNEACRIGLVDSYTYIGDPAFYDLPVEEMISDEFAAERAALIDMDNM
                     QAMESVPLSDLPVTKLEPTAEESQHTTHIAVIDQYGNIVSTTNTLGNGWGCKFMAPGL
                     GFFYNSHIGNLDHENPDSPDYVMPGKRVRSTISPSLVLQDGNPIMAVGSPGSLAIPPA
                     VATIINNVLLYGMNVQQAINEPRAMAINRSGMDSPARVSIEQPRFDPDLVAQMEGIGY
                     EMKDVGEYNMAVGGIAAIYLDRDNGIFYAGADPRRGYKALAY"
     gene            2483..3787
                     /locus_tag="ACZPAQ_02045"
     CDS             2483..3787
                     /locus_tag="ACZPAQ_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR0902790.1"
                     /translation="MNLTEAYLLLAVMLAVVAVVSVLFKGKSGLIAPLIFVAGLITAL
                     VAGLGFRLREVTEGPFVFVDTLMWVLCGAAFSYLLYVNGTFQFLFAKVVGKKRSPAAQ
                     MFILILFIGLPGMITGTALASVATTGLMAGRYLLDKGMEKSKVVEVVTVGALMGMLLP
                     PLCVPAMAPTIARQGLYPGAFEGYFLPILILALPALIVYCALAGRRVLGEWEADGNVE
                     KTGSAVCLIPLAVVAVLVLCYNFLYFLIPYYGGYPVIYLIGFVLALIFKCKGANPLIA
                     AADGIRTVAVELALILAYASVVETFNLVGVNGTLSAQMEIAGINGAVIALVLGLLVLA
                     GGLLLGTPFAYVVGALATYLVSNSNYGSYELSMMAVGAAIAMSMFLALRGSLAETVGE
                     TLGVTGVTGTEVVKRNIIFTLVLTAAIVVFLVAGSSLKFLMI"
     gene            3801..3956
                     /locus_tag="ACZPAQ_02050"
     CDS             3801..3956
                     /locus_tag="ACZPAQ_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902791.1"
                     /translation="MIAALFIIYRIIVAVLALVVIWDIVRERSLAHVMSLGIICFPLI
                     LRALLIK"
     gene            3970..5121
                     /locus_tag="ACZPAQ_02055"
     CDS             3970..5121
                     /locus_tag="ACZPAQ_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902792.1"
                     /translation="MKNKSLIPNIAVLLVVALVCVLTGQIYLQQQKDDVLYTENPDIT
                     GVIRLSDYNPNLKDTPGDVDIYVFDSGIPGGKALIYGGTHTNEVGSMLNAVTYLENVK
                     CEEGTLYVMVRANNSGYSHTQPLLAQANKMTFQLSDGSTREFRIGTRISNPVHQWPDP
                     NYRLNTSKRELRHDEIAEIRNLNRNHPGLEEGYLTEMVCYAIYNFIQTEQIDFIYDGH
                     EAGAESVKLVNTLVVHENAMPLGSSAVMNCVINNLPLKMEISGQTSYGLSHRGLGDNT
                     DALCTLFETCNPAMGSYHDKVTEELVKDGISGNYLAMHRAGLFPNSYDFDESGWPIEK
                     RTAYHMSMSAEIINTFDQMYPDKPLKISGLPDANEMIANGLEATLKSLA"
     gene            5202..5915
                     /locus_tag="ACZPAQ_02060"
     CDS             5202..5915
                     /locus_tag="ACZPAQ_02060"
                     /inference="COORDINATES: protein motif:HMM:NF040376.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HIR0902793.1"
                     /translation="MKKRNVCMLLAAALSCSLLLTACGGGGTSTTTPGTSTPSTSTSA
                     PAGSGGASTGAESGYTFRTSESNRVVTENGTFTKDQVSAPVLITSFGQSADVSMLDAL
                     MKKVGAEYTFDATASAEDAAGYKTIIIASGASSKGLGAAGISQEDEIARAEAIAQTAK
                     DNDITVIVAHMGGAARRGTLSDQFSDLALGCADYIMVVEEGNDDGKFSDAATDAGIPI
                     TMLYTIADAVTPLQEIFGA"
     gene            6046..>6249
                     /locus_tag="ACZPAQ_02065"
     CDS             6046..>6249
                     /locus_tag="ACZPAQ_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902794.1"
                     /translation="MEFELVIFLVMLGVFLLGNFLLKLPVSLSMAAGAIAGALVGGEG
                     IPLRHLFEGTFVYMDTVLIISMAM"
BASE COUNT         1219 a         1989 c         1910 g         1131 t
ORIGIN      
        1 cctggtggtc aaggacggcg agcctatcat ggccatcggc tcccccggca gctggtgcat
       61 cccgcctgcc atcgtccaga tcctcaatgc agtgctgctg ttcgacatgg acctgcagca
      121 ggccatcaac gagccccggg ccatcttcac ctcgtacagc gccccgctga aggtgtacgc
      181 cgagcctcgg ctgccggagg agaccatcca ggccctgatc gacgcgggct atgaggtgga
      241 ctccggaaag gactggaaca ccagcctggg cagtgtgggc gtggtcctgg tggacccgga
      301 cgacggcttt gtctatgcgg gcggagataa ccgccggcag tacaagtcac tggcctattg
      361 agaaagccag cgcatacaga taccaaaagt ctgatgggca tttgaaacgg aatgagaagg
      421 gaggaaaaga aggcccatga ggagatgtat gcgaggtaaa aaatgttaca tcaataagga
      481 ggagagcata tgaaacggaa taatcagtcc ctgacaacga aattcagcgc actggctctt
      541 gtacttgcaa tgatgctgtc cctgctggcg ggttgcggac agaccaccca gccggcagcc
      601 agctccaccc ctcccgcggc ttcttcagag cccgctgcct ctgagccgga gacccccgag
      661 atccctgaga ccatggaggg cgatgagttc ggaaacggtg ccatcggcac ccatggcggc
      721 gtatccagcg gcagcgtgta cgcctctcag gctggcctgg agatcctgca ggccggcggc
      781 aacgccgtgg acgcggcggt ggccaccgct ttcgcagtgg gcgtgtgtga gcccaacctg
      841 agcggcatcg gcggctgcgg catgatgaac atctatctgg cggataacca cgagtacgag
      901 atcctggagt acatggagac tgtccccgtg gcggtggagc ccggctggta caatccggag
      961 acagactctg ccacggccaa gaatgccgcc gtccccggtc aggtggctgg tctgctcacc
     1021 gccctggaga agtatggcac catgaccccg gaagaggtca tggcccccgc catcaagctg
     1081 gcccgggagg gcttccccat cgaggagcgt ctggccaatg ccatcatgga ctcctttgat
     1141 atgatctccg ccaatgagga ggctgcggcc atctacacca acgacggcct cccctatgcg
     1201 accggcgatc tgttcaagaa cgagcccctg gccgacaccc tggaggccat tgccaagggc
     1261 ggcatcgagg agttctatac cggcgagctg gctcagaaga tggtggacgg tctccaggcc
     1321 aatggcagcc tgatggccat ggaggacctg gcgacctaca ccgtggcgga gcgggagccc
     1381 atccgtaccg agtactacgg ctatgaagtg gtcaccgttc cccctccctc caacggcggc
     1441 gattggctgc tggagatgct gaacatcatg gaggagaagg acatctctca gtatgacccc
     1501 agcactctgg agtaccagta catcttcaac gaggcctgcc gcatcggctt ggtggattcc
     1561 tacacctaca tcggcgaccc cgccttctat gacctgcctg tggaggagat gatctccgac
     1621 gagttcgccg ccgagcgggc tgcccttatc gacatggaca acatgcaggc catggagtct
     1681 gtgcccctca gcgacctgcc ggtcaccaag ctggagccca ccgcggagga gagccagcat
     1741 actacccaca tcgccgtcat cgaccagtac ggcaacatcg tctccaccac caacaccctg
     1801 ggcaacggct ggggctgcaa atttatggcc cccggtctgg gcttcttcta caacagccac
     1861 atcggcaacc tggaccatga gaaccccgac agccccgact atgtgatgcc cggcaagcgg
     1921 gtgcgctcca ccatctctcc cagcctggtg ctccaggacg ggaaccccat catggcggtg
     1981 ggctcccccg gctccctggc catcccgccc gcagtggcca ccattatcaa caacgtgctg
     2041 ctgtatggca tgaatgtgca gcaggccatc aatgagcccc gcgccatggc catcaaccgc
     2101 tccggcatgg actcccccgc ccgggtgagc atcgagcagc cccgcttcga tccggacctg
     2161 gtggcccaga tggaggggat cggctacgag atgaaggacg taggcgagta caacatggcg
     2221 gtaggcggca tcgcagccat ctatctggac cgggacaacg gcatcttcta tgccggcgcc
     2281 gaccccagac gcggctataa ggccctggcc tattaagacc cggcattccg cgggcccggt
     2341 ccggcttcct gccggctgaa cgggaacgga cggccctccc cggctctccg gggagggcac
     2401 gggccccgat ctccgattgg ctgatccggt ccggggaccc cagatacccg gccggaatgc
     2461 gtgttacaag gagtggtaat gagtgaattt gactgaagcg tatctgctgc tggctgtcat
     2521 gctggctgtc gtagcggtgg tcagcgttct gttcaaaggg aaaagcggcc tgatcgcgcc
     2581 ccttatcttc gtggcgggcc tgatcaccgc gctggtggcc gggctgggct tccggctcag
     2641 agaggtcacc gagggtccct tcgtctttgt ggacaccctg atgtgggtgc tgtgcggcgc
     2701 agccttctcc tatctgctgt atgtcaacgg gaccttccag ttcctgttcg ccaaggtggt
     2761 agggaagaag cgcagcccgg ccgcccagat gttcatcctg atcctgttca tcggcctgcc
     2821 cggcatgatc accggcacgg ccctggccag cgtggccacc accggcctga tggctggccg
     2881 ctacctgctg gacaagggga tggagaagag caaagtggtg gaggtggtga cagtgggcgc
     2941 tctgatgggc atgctgctgc ctcccctgtg cgtacccgcc atggccccca ccattgcccg
     3001 gcaggggctc tatcccggcg cttttgaggg atattttctc cccatcctga tcctggccct
     3061 ccccgccctg atcgtctact gcgccctggc cgggcgccgg gtgctggggg agtgggaagc
     3121 ggacgggaac gtggaaaaga cgggcagcgc ggtctgcctg atccccctgg ccgtggtggc
     3181 ggtgctggtg ctctgctaca acttcctgta cttcctgatc ccctattacg ggggttatcc
     3241 ggtgatctac ctcatcggct tcgtgctggc cctgatcttc aagtgcaagg gagccaaccc
     3301 cctgatcgcg gcggcggacg gcatccgcac cgtggcggtg gaactggccc tgattctggc
     3361 ctatgcctct gtggtcgaga ccttcaacct ggtgggcgtc aacggcaccc tctccgccca
     3421 gatggagatc gccggcatca acggcgcagt gatcgccctg gtactgggac tgctggtgct
     3481 ggcgggcggt ctgctgctgg gaaccccctt cgcctatgtg gtgggggcgc tggccactta
     3541 tctggtcagc aactccaact atggaagcta tgagctgtcc atgatggcgg taggcgccgc
     3601 catcgccatg agcatgttcc tggccctgcg gggcagcttg gcggagacgg tgggcgagac
     3661 cctgggggtg actggggtca ccggaactga ggtggtcaag cgcaacatca tcttcacgct
     3721 ggtgctgacc gccgccatcg tggtgttcct ggtggcaggt tcatcgctga agttcctgat
     3781 gatttaaggg gggacgaaga atgattgctg cattgttcat catctatcgg atcatcgtcg
     3841 ctgtcctggc cctggtggtg atctgggaca tcgtgcggga gagatcgctg gcccatgtga
     3901 tgagtctggg gatcatctgt ttccccttga tcctccgcgc gctgctgatc aaatgaaggg
     3961 ggaacggaaa tgaaaaacaa gagtctgatc ccaaacattg ccgttctgct cgtggtggcc
     4021 ctggtctgcg tgctgacagg gcagatctat ctccagcagc agaaggacga cgtgctgtac
     4081 accgaaaatc ccgacatcac cggtgtgatc cggctgagcg actacaatcc caacctgaag
     4141 gacacgccgg gcgacgtgga tatctatgtc tttgacagcg ggatccccgg aggcaaggcg
     4201 ctgatctacg gcggcaccca caccaatgag gtgggctcca tgctcaacgc cgtgacctat
     4261 ttggagaacg tgaagtgtga agagggcacc ctctatgtga tggtgcgggc caacaacagt
     4321 ggatacagcc acacccagcc cctgctggcc caggccaaca agatgacctt ccagctgtcc
     4381 gacggctcca cccgggagtt ccgcatcggc acccgcatct ccaacccggt gcaccagtgg
     4441 cccgacccca actaccggct gaatacctcc aagcgggaac tgcgccacga cgagatcgcg
     4501 gagatccgca acctgaaccg caaccacccc ggactggagg agggctacct caccgagatg
     4561 gtgtgctacg ccatctacaa ctttatccag accgagcaga tcgacttcat ctatgacggc
     4621 cacgaggccg gtgccgagtc ggtgaagctg gtcaacaccc tggtggtcca tgagaacgcc
     4681 atgcctctgg gctcctccgc tgtgatgaac tgcgtcatca acaacctgcc cctgaagatg
     4741 gagatctccg gccagacctc ttacggcctg tcccaccggg gcctgggcga caataccgac
     4801 gccctgtgca ccctgttcga gacctgcaac cccgccatgg gcagctacca cgacaaggtg
     4861 acagaggagc tggtgaagga cggcatctcg ggcaactatc tggccatgca tcgggcgggg
     4921 ctgttcccca attcctacga ctttgacgag agcggctggc ccattgagaa gcggaccgcc
     4981 taccacatga gcatgagtgc ggagatcatc aacaccttcg accagatgta ccccgacaag
     5041 cccctgaaga tctccggcct gcccgatgcc aacgaaatga tcgccaacgg cctggaggcc
     5101 accctgaagt ccctggcctg agggactcga ctggtaaggt gccaagtccc ggaggaccgg
     5161 gatcaagcat agaactgaaa tgatcttagg aggaacttaa aatgaaaaag cgcaacgtat
     5221 gtatgctcct ggccgcggcc ctcagctgca gccttctgct gaccgcctgc ggcggtggcg
     5281 ggaccagcac caccaccccg gggaccagca ccccgtccac cagcaccagc gcccccgccg
     5341 ggagcggcgg cgcctccacc ggtgctgaga gcggctacac cttccgcacc agcgagagca
     5401 accgggtggt gacggagaac ggcaccttca ccaaggacca ggtctccgcc cccgtcctaa
     5461 tcacctcctt cggccagagc gccgacgtat ccatgctgga cgccctgatg aagaaggtgg
     5521 gagctgagta caccttcgac gccaccgctt ccgcagagga cgccgccgga tacaagacca
     5581 tcatcatcgc cagcggagcc tcctccaagg ggctgggtgc ggccggcatc tcccaggagg
     5641 acgagatcgc ccgcgctgag gccatcgccc agaccgccaa ggacaacgac atcaccgtga
     5701 tcgtggccca catgggcggc gctgcccgcc ggggcactct gtctgaccag ttcagcgacc
     5761 tggccctggg ctgtgccgac tacatcatgg tagtggagga gggcaacgac gacggcaagt
     5821 tctctgacgc cgccaccgac gccggcatcc ccatcaccat gctgtacacc atcgccgacg
     5881 ctgtgacccc gctccaggag atcttcggcg cctgaacccc attgcactgc aaggaggccc
     5941 cgcctgtcaa aacgggtggg gcctcttccc aaaagcggac gctccgccgg cagaggcggc
     6001 cgtctgcccg actcccgcga atactccgga acaggaggga tttgattgga atttgaactg
     6061 gtaatattcc tggtcatgct gggcgtgttc ctgctgggga acttcctgct gaagctgccg
     6121 gtcagcttgt ctatggcggc cggcgctatc gccggcgccc tggtgggtgg ggagggtatc
     6181 cccctgcgcc atctgttcga gggcaccttt gtctatatgg atacggtgct catcatctcc
     6241 atggccatg
//
LOCUS       DBMDSJ010000049         6247 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3632_length_6247_cov_1.752034, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000049 DBMDSJ010000000
VERSION     DBMDSJ010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_6247_cov_1.752034"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(139..1521)
                     /locus_tag="ACZPAQ_02070"
     CDS             complement(139..1521)
                     /locus_tag="ACZPAQ_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013279.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR0902795.1"
                     /translation="MDDFFADYMDQAMEQVRWRRARPALAQELRTHLLDQRDACLAQG
                     MSQTEAEAESLRQMGDPVEVGTQLDRVHRPAPQWGMLALVGLLLCSGFFIQATVMQQL
                     GRVQPYMPTVIVSILLGVAAMAVVYLGDYTLLGRRPVLLYGLGMTGLVLLTVTSEVKA
                     QGVKLDAISYLLLGPVLYALLVYGLRGKGWRGLLLALAGLVPPVVLPCLIPNFTSGAI
                     AGLSGAAVVLTAAWKGWMGVPRKKGAAAVAVGLLVLNGAALWHLSQSPYALERVRFAL
                     HPELDPLGLGYFPMMVREMLSGAQWLGKGELGELSGAAGYLPGVSDYWLARVIHELGW
                     AAGLALLALMTALFALGLRRCMRQRGMLGRLLSTAALTTLVLETVCFVVYDLGFPLAA
                     PLVLPFLSTGGRYMVVNLLLLGLMLSAFREERLPQGQKGPAAQSSRGRPLISWRDGDL
                     VIALGRRTGK"
     gene            complement(1514..1852)
                     /locus_tag="ACZPAQ_02075"
     CDS             complement(1514..1852)
                     /locus_tag="ACZPAQ_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR0902796.1"
                     /translation="MGIDKGWMAGSTAMLLLRLLEERDRYGYQMIEELRRRSDHTFDL
                     KAGTLYPLLHGLEEKGWITSYQGEGGGRTRKYYHLTEKGGRELKEKRAEWDRYTRAVS
                     RVLEGGVCFG"
     gene            complement(1924..2574)
                     /locus_tag="ACZPAQ_02080"
     CDS             complement(1924..2574)
                     /locus_tag="ACZPAQ_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0902797.1"
                     /translation="MSLERARQAALVQAALRGDEHAREELGALAEEYLRRCFFRHARR
                     CYGDGVKEELEDIASDAVEKCLARLDRFQGGRALFTTWVYGFAKNVLRNHHRKRARRD
                     QIWEEVRKDQSAWWHEPPPDEPHFGGDPLRWVIHQEKLEALHLAMADLPVNDEVILTL
                     RVAKARPEAETARQLKLEDEEAEKLYRCALELLRERFFRYYYGVPRRKRVGQPKSA"
     gene            complement(2599..4044)
                     /locus_tag="ACZPAQ_02085"
     CDS             complement(2599..4044)
                     /locus_tag="ACZPAQ_02085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR0902798.1"
                     /translation="MGHLLCFYLLELGMAAAWCRWVICREVDLPALLRTARTVWSGER
                     QVLGELLLVRRRWTEGEETERFRQAWRRIALRLLPPLLYLTLGGALAPVLAWRMFPEG
                     AGQLAMGCCVLAGLAGGCFKAGTQRTVEELSWMQGELEGLRSRPGFLKEHTRPVEWDQ
                     KKRGLLVLLLLAAAAVFGTIWGLSHSLAAAGPVGEAAALVLLALLWLLSEQPWRKKKG
                     RSAAAEPGVTIQGSSLERYQEALEDFCRAMKIPALLVTETQEGTVNASARLSPGQVPE
                     LRVTGGLLKQAELLFQRTGNLEQMDRTVLSILGHELSHIAHRDALQLERRARKAALVS
                     GGIFLLLAGSVLLLPAAAPLPALLLVLYLGADLLFGKILTDRRFWGQMCELRADRTGL
                     RLSGASGEEWMQFWEIARLLQAEEKRQARLRGENVLYQGYRRYLRVEFHPSLRRRRAM
                     MERGRPWGWRDYLDQLWYLRMALWQGRGWNE"
     gene            complement(4132..5964)
                     /gene="glmS"
                     /locus_tag="ACZPAQ_02090"
     CDS             complement(4132..5964)
                     /gene="glmS"
                     /locus_tag="ACZPAQ_02090"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR0902799.1"
                     /translation="MCGIVGYIGTEEAAPILLEGLRRLEYRGYDSAGLAVLDPEQGLQ
                     VAKAKGRLQVLADLTGQGQKLTGHMGLGHTRWATHGAPNDVNSHPQVSQSGRIAVVHN
                     GIIENYARLKEFLESKGIAFVSETDTEVVAQLLDYYYEGDLLDAVGKVLHRIEGAYAL
                     GIVCADEPDRLVAVRKDSPLILGYGANFHMLASDVTAVIQHTREVCYLEDGEVAVLTA
                     HGAQVYNSLLQPVEKEISHVDWAISAAEKGGYEHFMFKEIMEQPKALRDTIFPRLRDG
                     EVVLDEVSLTREELERLDRLYVIACGSSYHVGVAAKYTLERLLRIPVEVTLASEFRYC
                     DPIVTRRTLAVVISQSGETIDTLAAMREARRLGARLLSIVNVVGSTIARESDDVIYTW
                     AGPEIAVATTKAYSTQLAVIYLLGLRFAHLLGTVGEEEYAGMVAQLRQLPAKVEEILS
                     DTEKIQYYASIYFNHDSVFYIGRNIDYAVGLEGSLKLKEISYIHSEAYAAGELKHGTI
                     SLIEDGTLVVALASWGDLFDKLMSNVKEVKARGADVVGITTRSHGAELAKSVDSAIQI
                     PDTHPMFLPSLEVVPMQLFAYYVALMRGCDIDKPRNLAKSVTVE"
BASE COUNT         1109 a         2272 c         1752 g         1114 t
ORIGIN      
        1 ccggaggttt tgtctgtctg aggcatcttt tgtatgggat gtcccttccc agacacatgt
       61 cccgggaaaa aacaaaattc cagataatcc ccgctggagg gccttttatt caagatgcgt
      121 cggagacttc ccctgcggtc attttcccgt ccttctgccc aaggcgatga ccagatcccc
      181 gtccctccag gagatcagag ggcggccccg gcttgactgt gcggcgggcc ccttctgccc
      241 ctgcgggagc ctctcttccc gaaaggcgga cagcatcagc cccagcagca gcagattgac
      301 caccatatac cgcccgccgg tggacaggaa ggggagcacc agcggcgcgg ccagggggaa
      361 tcccagatca tagaccacaa agcacactgt ctccaggacc agagtcgtca gggccgcggt
      421 ggacagcagg cgtcccagca tccccctctg ccgcatacag cgccgcagcc ccagtgcgaa
      481 gagggcagtc atcagggcca gaagggccag cccagcggcc cagcccagct cgtggatcac
      541 ccgggccagc cagtaatccg aaacgccagg gagatagcca gccgctccgc tcagttcccc
      601 cagctcccct ttccccagcc attgggcgcc ggagagcatc tcccggacca tcatggggaa
      661 atatcccagc cccagggggt ccagctccgg gtggagggcg aatcggaccc gctccaacgc
      721 ataagggctt tgagacagat gccacagcgc ggccccgttc aataccagga gcccaacggc
      781 cacggcggct gcccccttct tccggggcac ccccatccag cccttccacg ccgcggtgag
      841 caccaccgct gctccgctga ggccggcgat ggcaccgctg gtgaaattcg ggatgaggca
      901 gggcaggacc accggcggga ccagccccgc cagggccagt agcagtcccc gccagccctt
      961 ccctcgcagg ccatagacca gcagggcgta gagcaccggc cccagcagga gatagctaat
     1021 cgcatccaac ttgacgccct gggccttgac ttcactcgtc accgtcagga ggaccaggcc
     1081 ggtcatgccc agcccataga gaaggaccgg acgccgtccc agcagggtat agtcccccag
     1141 gtacaccacc gccatagcgg ccacccccaa gagaatgctt acgatcacgg taggcatata
     1201 gggctgaact ctgcctagct gctgcatcac ggtggcctgg atgaaaaatc cggagcacag
     1261 cagcagccca accagggcca gcatgcccca ctggggcgca ggccggtgca cccggtccag
     1321 ctgggtaccc acctccaccg ggtcccccat ctgccgcagg ctctccgcct ccgcctccgt
     1381 ctggctcatc ccctgggcca ggcaggcgtc ccgctggtcc agcaggtggg tgcgcagctc
     1441 ctgggccaga gcgggccggg cgcgcctcca gcgcacctgc tccatggcct gatccatgta
     1501 atccgcaaaa aagtcatcca aaacagacgc ccccctccag cacccgggac accgcccggg
     1561 tgtagcggtc ccactccgcc cgcttctcct tcagctcccg ccctcccttt tctgttaagt
     1621 gataatactt tcgggttctt ccgcccccct ccccctgata ggaggtaatc caccccttct
     1681 cctccagccc gtgaagcaaa gggtaaagtg ttcctgcttt caagtcaaac gtatggtctg
     1741 agcggcggcg gagctcctcg atcatctgat agccgtaccg gtctctctcc tccagcagcc
     1801 ggaggagcag catcgctgtg ctcccggcca tccatccttt atcgatgccc atagatagac
     1861 ctcctatata tcggatatct atatattaaa ggcggtccca tccttttgtc aaggacaatt
     1921 ttttcaggcc gacttcggct gccccactct cttccgcctg gggactccgt aatagtaacg
     1981 gaaaaaccgc tcccgaagca gctccagggc gcaccgatag agcttttccg cctcctcatc
     2041 ctccagcttc agctgtctgg ctgtctctgc ctcaggccgt gcctttgcca ccctcagcgt
     2101 caaaatgacc tcatcgttga ctggaagatc cgccatagcc aagtggaggg cctccagttt
     2161 ctcctgatgg atcacccacc tcagcgggtc gccgccgaag tggggctcat ccggtggcgg
     2221 ctcatgccac catgcactct gatccttccg cacctcctcc cagatttgat ctcggcgtgc
     2281 cctcttcctg tggtggttcc gaagaacatt cttcgcgaac ccatagaccc aggtggtgaa
     2341 cagagctctt cccccctgaa accggtccag ccttgccagg catttctcca ccgcatcgga
     2401 tgcgatgtcc tccaactcct ctttcacacc gtccccatag caccgccgtg cgtgccggaa
     2461 gaaacagcgg cgcaaatatt cctctgccag cgcgcccaac tcctcccggg catgctcgtc
     2521 tcctcgcagg gcagcctgga ccagggcggc ctgccgggcc cgctccaaac tcatatgcgg
     2581 ctctcctccc catgtctctc actcattcca gcccctcccc tgccacagag ccatccggag
     2641 ataccagagc tgatccagat agtcccgcca cccccagggc cgcccccgct ccatcatggc
     2701 tcttcgccgc cggagggaag ggtggaactc cactctgaga tagcgccggt aaccttggta
     2761 cagcacattt tcccctctca gccgcgcctg ccgcttctcc tccgcctgga gcagccgcgc
     2821 gatctcccag aactgcatcc actcctcccc ggacgccccg ctcaggcgca ggccggtccg
     2881 gtccgcccgc agctcacaca tctgtcccca gaaccggcgg tccgtcagga tcttcccaaa
     2941 gagcaggtct gcccccagat agaggaccag caggagagcg ggcagtgggg cagccgccgg
     3001 caggagcagg accgatcccg ccagcagcag gaagatgcct ccgctgacca gtgccgcctt
     3061 ccgtgcccgc cgctccagct ggagggcatc ccggtgggcg atgtggctca gctcatggcc
     3121 caggatggaa agcactgtcc ggtccatctg ctccaggttt cccgtccgct ggaagaggag
     3181 ctcagcctgc ttcagcagtc ccccggtcac ccgcagctcg ggcacctggc ctggggagag
     3241 gcgggcgctg gcgttgaccg tcccctcctg ggtctccgtt accagcaggg ccgggatctt
     3301 catcgcccgg cagaagtcct ccagcgcctc ctgataccgc tccaggctgc tcccttggat
     3361 ggtcacgccc ggctctgctg ctgcgctcct cccctttttc ttccgccagg gctgttctga
     3421 gagcagccac agcagggcca gcagaaccag agccgccgcc tctcccaccg gaccggcggc
     3481 ggccagactg tggctcagcc cccagatggt tccaaaaacg gccgccgcag ccagcaggag
     3541 caagacaagg agccctctct ttttctggtc ccactccacc ggccgcgtgt gctctttcag
     3601 aaacccaggt cgggaacgca ggccctccag ctccccctgc atccagctta gctcctctac
     3661 ggtcctctgg gtccccgcct tgaagcagcc gcctgccagc cccgccagca cacagcagcc
     3721 catcgccagc tggccggccc cctccggaaa cattctccag gccagcactg gggccagcgc
     3781 cccacccagg gtgaggtata gcagaggcgg gagcaggcgc agggcgatcc tgcgccaagc
     3841 ctggcggaag cgctctgtct cctccccctc tgtccagcgg cggcggacca gcagcagctc
     3901 ccccagcacc tgccgttccc cgctccacac ggttcgggcg gtgcggagca gggcgggcag
     3961 gtctacctcg cggcagatga cccaccggca ccaggctgcc gccatcccca gctccagcag
     4021 ataaaaacac agcagatgtc ccatctctct tttcctccgc aaaaatgcgc gcgcccccct
     4081 ctgcgtcaga aggagacgcg cgctcctgtt tttctgtgca gccccgggcc gtcactccac
     4141 cgtgacgctt ttcgccagat tccggggctt gtcgatgtca cagccccgca tcagggccac
     4201 atagtaggcg aacagctgca tgggcaccac ctccagggag gggaggaaca tggggtgggt
     4261 gtccgggatc tggatggcgc tgtccacgct cttggccagc tccgccccat ggctgcgtgt
     4321 ggtgatgcca accacgtccg ccccccgggc ctttacctcc ttcacattgc tcatcagctt
     4381 gtcaaacagg tccccccagc tggccagggc cactaccagg gtgccgtcct cgatgaggga
     4441 gatggtgccg tgcttcagct ccccggcggc gtaggcctcc gagtggatgt aggagatctc
     4501 cttcagcttc aaagagccct ccagccccac cgcatagtcg atgttccgcc cgatgtagaa
     4561 cacggagtcg tggttgaagt agatagaggc gtagtactgg atcttttctg tgtcggacag
     4621 gatctcctcc accttggcgg ggagctgcct cagctgggct accatgccgg cgtactcctc
     4681 ctcccccact gtgcccagca gatgggcgaa acgcaggccc agcagataga tgaccgccag
     4741 ctgggtggag tatgcctttg tggtggctac ggcgatctct ggcccggccc aggtgtagat
     4801 cacgtcgtcc gactcccggg caatggtgga ccccaccaca ttgacgatgg acagcagccg
     4861 ggctcccagc ctccgggcct cccgcatggc ggccagggta tcgatagtct cgccggactg
     4921 gctgatgacg acggccaggg tgcgccgggt gacgatgggg tcacagtagc ggaactctga
     4981 ggccagtgtc acctccaccg ggatgcgcag cagccgctcc agggtgtatt tggccgcaac
     5041 ccccacatgg taggaggagc cgcaggcgat gacgtacagc cggtccagcc tctccagctc
     5101 ctcccgggtg agggagacct cgtccagcac cacttcccca tcccgcaggc gggggaaaat
     5161 ggtgtcccgc agggccttgg gctgctccat gatctccttg aacataaagt gctcatagcc
     5221 ccccttttcc gcggcagaga tcgcccaatc tacatgggag atctccttct ccaccggctg
     5281 gagcagggag ttgtacacct gggccccatg ggcggtaagc accgccactt ccccatcctc
     5341 cagatagcac acctcccggg tgtgctggat gacggcggtg acatcagagg ccagcatgtg
     5401 gaagttcgcc ccgtatccca agatcagggg cgagtccttc cgcactgcca ccagccggtc
     5461 tggctcgtcg gcgcagacga tgcccagggc ataagccccc tcgatgcggt ggagcacctt
     5521 gcccaccgcg tccagcaggt cgccttcata gtagtagtcc agcagctggg ccaccacctc
     5581 cgtatctgtc tccgagacga aggctatccc cttggactcc aaaaactcct tcagccgggc
     5641 atagttctcg atgatcccgt tgtggaccac agcgatccgg ccggactggc tcacctgggg
     5701 gtgggagttg acgtcgttgg gcgccccgtg ggtagcccag cgggtatgcc ccagccccat
     5761 gtgtccggtc agcttctgcc cctgaccggt gaggtcggcc agcacttgga gccgcccctt
     5821 ggccttggcc acctggagcc cctgctctgg gtccagcacc gccagccccg cggagtcata
     5881 cccccggtac tccaggcggc gcagcccctc cagcaggatg ggggccgcct cttccgttcc
     5941 aatatatcct acgataccac acattgcaat gatgatcctc ctgaattgaa atggaggaca
     6001 aactcgcagc catttgcacc tcgttccccg gtcacggccc ctctgttttg cggtcgcccg
     6061 catatttcga gccatgtcac gcgcccggag cggcgcggaa gggcatccgc cgaaccggag
     6121 cgccccctgt gccggcggcg ctccccctcg atactccctt cccctcgtcg tccctccccg
     6181 ggcgttctgc cgggaagggc gctggcgctt ctgatgggcc agagcccatg gtcctccttt
     6241 ctctctg
//
LOCUS       DBMDSJ010000050         6184 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3666_length_6184_cov_1.894624, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000050 DBMDSJ010000000
VERSION     DBMDSJ010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3666_length_6184_cov_1.894624"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="ACZPAQ_02095"
     CDS             <1..761
                     /locus_tag="ACZPAQ_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR0902800.1"
                     /translation="DPDEFIKKYGPEAFARLLDKSENHIDYRLEQLRGRYDLSDDSQR
                     VEFLREATGVIASLHSAVEREIYGGHAAQMAGISAEAMAQEVKKELRRRIQKEKKQQE
                     RRDLSPAIQLQPKARGMRYDNIRSARAEEGVLRLILLDSSLLEETEGLTGSEFSSPLL
                     GRIFDLLRSRAAEGLSTQLPALAGALEPEEMAHLAWVAEQPENLANSRRALADYIALI
                     REEGLRRSGTDEEALLRAAQKRYQETKAYMEEKP"
     gene            854..2065
                     /gene="rpoD"
                     /locus_tag="ACZPAQ_02100"
     CDS             854..2065
                     /gene="rpoD"
                     /locus_tag="ACZPAQ_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR0902801.1"
                     /translation="MKVVGGIPGAEKLAELIERGKKKGNLSAAELMDVLEDMDLESEQ
                     MDKIYDTLENLGIDTVGEDYIPELPDDVEPTIEAIEELPEEEIVDPNTMVDAFGTDDP
                     VRMYLKEIGKVNLLTSDEEIDLAQAMGAGAEAKEQMEELESSGEEIPAEVRTELEKAI
                     KKGERAKQRLAEANLRLVVSIAKRYVGRGMQFLDLIQEGNLGLIKAVEKFDYTKGYKF
                     STYATWWIRQAITRAIADQARTIRIPVHMVETINKVIRVSRQLLQELGHDPTPEEIAD
                     EMNMPVDRVREILKIAQEPVSLETPIGEEEDSHLGDFIPDEDASEPAEAASFTLLKEQ
                     LVDVLSTLTPREEKVLKLRFGIEDGRTRTLEEVGKEFNVTRERIRQIEAKALRKLRHP
                     SRSKKLKDFLN"
     gene            2401..4233
                     /gene="recQ"
                     /locus_tag="ACZPAQ_02105"
     CDS             2401..4233
                     /gene="recQ"
                     /locus_tag="ACZPAQ_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HIR0902802.1"
                     /translation="MTKQDVLKQYFGYDTFRGGQEEIIDALLAGRDVLAVMPTGAGKS
                     VCYQVPALLLPGITLVVSPLVSLMRDQVTQLVQMGVPAAYLNSSLTYRQYLLALDRAR
                     EGRYKIIYVAPERLDTEGFQSFVRSAQISMVAVDEAHCISQWGQDFRPAYLNIPDFVD
                     RLPQRPVVGAFTATATSEVREDIQRLLELEAPLSITTGFDRDNLYLEVCRPSVKRVEL
                     LARVREQPGECGIVYCSTRKNVEEVCQFLQSSGISATRYHAGLEPEERQQNQEDFLFD
                     RVQVMVATNAFGMGIDKSDVRFVIHYNMPKDLESYYQEAGRAGRDGAEARCILLYSGQ
                     DVRTAQFLISQSEPSEQLDPETARQLQERDLGRLKQMTFYCRTRRCLRQYILNYFGQS
                     APDYCGACYNCLHNFQEIDVGEDARAILRCVAETGQRFGAGLIAAVLCGGENEKIRRY
                     RLERERTYGALAALSQREVQDRIRFLVDEHILELTDGQYPVLRCGERAEDVAYSDGPL
                     MMKAVREERPAADRRRAEDSPLTERQQALFQRLREERAHLARLRGVPAFVIFSDKTLR
                     EMAAAQPRDLRQLQNVGGVGSFKAEQYGAQFLEIISDFQETAAI"
     gene            complement(4284..4460)
                     /locus_tag="ACZPAQ_02110"
     CDS             complement(4284..4460)
                     /locus_tag="ACZPAQ_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HIR0902803.1"
                     /translation="MSQLLPTIIVSLILAGVVALAVRSLWQSHKSGGHCNGDCGTCGG
                     CGGHHTRCGPHAPK"
     gene            complement(4487..>6184)
                     /gene="feoB"
                     /locus_tag="ACZPAQ_02115"
     CDS             complement(4487..>6184)
                     /gene="feoB"
                     /locus_tag="ACZPAQ_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR0902804.1"
                     /translation="VFTGSVEHAIAHIEESIQGMVDDQYLRWYAIKVFERDEKVLESL
                     TLTKDLEAHLEAHIADCENELEDDAESIITNQRYAYINSVVNRAVKKKAPKHSLTTSD
                     KIDRIVTNRVLALPIFVAVMFLIYAIAMGGWAISIGTAATDFTNDTIFGEWVPGFFDS
                     FLLNASGEPVVADWLYGLIQDGIVAGVGAVLGFVPQMLVLFVLLAVLEDVGYMARIAF
                     IMDRIFRRFGLSGKSFIPMLVATGCGVPGIMASRTIEQDRDRKITIMTTGFIPCGAKM
                     PIIGLFAGAVFGNSAWVATSAYFIGVAAVIVSGIMLKKFKAFAGEPAPFVMELPAYHA
                     PSAGNVLRATWERGWSFIKRAGTVILISSIILWFLQGYGFVDGVFQAVEDNNDSLLAV
                     IGNAIAWIFYPLGWTGSMAWKAAVATFTGLIAKEEVVNTFGVLYHYAGDIDLMEDSSP
                     IWAMVGADFGAISAYSFMIFNLLCAPCFAAMGAIKREMHNTKWTLGAIGYMCLFAYVT
                     AMIVYQIGGLFTGEAAFSVFTVIAIALLAGIIFLLVRRGYQPSEEVRHLTDIPAGKK"
BASE COUNT         1237 a         1838 c         2144 g          965 t
ORIGIN      
        1 aggacccgga cgagttcatc aagaaatacg ggcccgaggc cttcgcccgt ctgctggaca
       61 agagcgagaa ccacatcgac taccggctgg agcagctgcg gggccggtat gacctgagcg
      121 acgacagcca gcgggtggag tttctgcggg aggccaccgg ggtgatcgcc tccctgcaca
      181 gcgcggtgga gcgggagatt tatgggggcc atgcggccca gatggcgggc atctccgccg
      241 aggccatggc ccaggaggtc aaaaaggagc tccgccgccg catccagaag gaaaaaaagc
      301 agcaggagcg gcgggacctg tcccccgcca tccagctcca gcccaaggcc agggggatgc
      361 gctacgacaa catccgctcc gcccgggcgg aggagggggt gctgcgcctg atcctgctgg
      421 acagctccct gctggaggag acggagggcc tgacgggttc ggagttctcc tctccgctgc
      481 tggggcggat ctttgacctg ctgcgcagcc gtgcggcaga gggactgagc acccagctgc
      541 ccgccctggc cggggcgctg gagccggagg agatggccca cctggcctgg gtggccgagc
      601 agccggaaaa tctggccaac agccgcagag ccctggccga ctacatcgcc ctcatccggg
      661 aggaggggct gcgccgcagc gggacagatg aggaagctct gctgagggcg gcgcaaaaga
      721 gatatcagga aacaaaagca tatatggagg agaagccatg agcaccaaga agacagagac
      781 ttccgccgct gtggaagaga agaagagcaa gaccgacatc gagacccgca tggaagcggg
      841 caaggtggag gtcatgaagg tggtgggagg catcccaggt gcggaaaagc tggccgaact
      901 gatcgagcgg ggcaagaaga agggcaatct gtcggccgcc gagctgatgg acgtgctgga
      961 ggacatggat ctggagtccg agcagatgga caagatctat gatactctgg agaacctagg
     1021 catcgacaca gtgggggagg actacatccc cgagctgccc gacgacgtgg agcccaccat
     1081 tgaggccatc gaagagctgc ctgaggaaga gatcgtggac cccaacacca tggtggacgc
     1141 cttcggcacc gacgacccgg tgcggatgta tctgaaggag atcggcaagg tcaacctgct
     1201 cacctccgat gaggagatcg acctggccca ggccatgggg gcgggcgccg aggcaaagga
     1261 gcagatggag gagctggaga gctccggaga ggagatcccc gccgaggtcc gcaccgagct
     1321 ggagaaagcc atcaaaaagg gcgagcgggc caagcagcgg ctggcagagg ccaacctgcg
     1381 cctggtggtc tccattgcca agcggtatgt gggccggggg atgcagttcc tggacctgat
     1441 ccaggagggc aacctgggcc tgatcaaggc ggtggagaag ttcgactaca ccaaggggta
     1501 caagttctcc acttacgcca catggtggat ccgtcaggcc atcacccggg cgatcgctga
     1561 ccaggcccgc accatccgca tcccggtgca catggtggag accatcaaca aggtgatccg
     1621 ggtctcacgc cagctgctcc aggagctggg ccacgacccc acgccggagg agatcgccga
     1681 cgagatgaac atgccggtgg accgggtgcg ggagatcctg aagatcgccc aggagcctgt
     1741 ctctctggag acccccatcg gggaggagga ggacagccac ctgggggact tcatccccga
     1801 cgaggatgcc agcgagcccg ccgaggccgc ctccttcacc ctgctgaagg agcagctggt
     1861 ggatgtgctc tccaccctga ccccccggga ggagaaggtg ctcaagctgc gcttcggcat
     1921 cgaggacgga cggacccgta ccttggaaga ggtgggaaag gagttcaacg tcacccggga
     1981 gcgcatccgc cagatcgagg ccaaggccct cagaaagctg cgtcatcctt ctcgttccaa
     2041 gaaactaaaa gactttttga actgatccgg ggatcgtttt gctgagtgtt ccggggcgca
     2101 gctttccggg gcccccgggc gggcccagcg cagcgggccg cacggggaga gccggagcga
     2161 cggaatgagt gagctcccgc ctccggcagg ggcgagggat atgcagtccg cgacggcgac
     2221 gcgtcatcct tctcgttcca agaaacgaaa agactttttg aactgacagg cgggcagagc
     2281 accggagggc cggggcgcag gtttcaggtg ccccacctgc aaaaaggacc ccagcggaac
     2341 acacatcccc cggagcgcgg ctccgggggt gtgctttgcg ggaaagagcg gagaacgact
     2401 atgacaaagc aggatgtttt gaagcagtat ttcggctatg acaccttccg gggcggtcag
     2461 gaggagatca tcgacgcact gctggccggg cgggatgtgc tggccgtcat gcccacggga
     2521 gcggggaaat cggtgtgcta tcaggtgcct gccctgctgc tgcccggcat caccctggtg
     2581 gtctccccgc tggtctccct catgagggac caggtgaccc agctggtgca gatgggagtg
     2641 cccgccgcct acctcaacag ctctctcacc taccggcagt atctgctggc cctggaccgg
     2701 gccagggagg gccggtacaa gatcatctat gtggctccgg agcggctgga taccgagggg
     2761 ttccagtcct ttgtccgctc ggcccagatc tccatggtgg cagtggacga ggcccactgt
     2821 atctcccagt gggggcagga cttccgcccc gcctatctga acatcccgga ctttgtggac
     2881 cggctgcccc agaggccggt ggtgggggcc ttcaccgcca ccgccacctc ggaggtccgg
     2941 gaggatatcc agcggctgct ggagctggag gcccccctgt ccatcaccac cgggtttgac
     3001 cgggacaacc tgtatctgga ggtgtgccgc ccctccgtaa agcgggtgga gctgctggcc
     3061 cgggtgcggg agcagccggg ggagtgcggc atcgtctact gctccacccg gaagaacgtg
     3121 gaggaggtct gccagttcct ccagagctcc ggcatctccg caacccgcta ccacgccggg
     3181 ctggagccgg aggagcggca gcagaatcag gaggacttcc tcttcgaccg ggtccaggtg
     3241 atggtggcta ccaacgcttt cgggatgggg atcgacaagt cggacgtgcg cttcgtcatc
     3301 cactacaaca tgcccaagga cctggagagc tactaccagg aggcgggccg ggcaggccgg
     3361 gacggggcgg aggcccgatg tatcctgctg tacagcgggc aggacgtgcg cacagcccag
     3421 ttcctcatca gccagagcga gcccagcgag cagctggacc cggagacggc gcggcagctc
     3481 caggagcggg acttgggtcg tctgaagcag atgacgttct actgccgcac ccggcggtgc
     3541 ctgcgtcagt atatcctgaa ctatttcggc cagtcggccc cggactactg cggcgcctgc
     3601 tacaactgcc tccacaattt ccaggagatc gatgtggggg aggacgcccg ggccatcctc
     3661 cgctgtgtgg cggagaccgg ccagcggttc ggcgccggac tgatcgccgc agtgctgtgc
     3721 ggcggggaga acgagaagat ccgccgttac cggctggagc gggagaggac ctatggagcc
     3781 ctggccgccc tgagtcagcg ggaggtccag gaccgcatcc gcttcctggt ggacgagcac
     3841 atcctggagc tcactgacgg ccagtacccg gtcctccggt gcggggagcg ggcggaggac
     3901 gtggcgtaca gcgacggacc gctgatgatg aaggccgttc gggaggagcg gccggcggcc
     3961 gaccgccggc gggcggagga cagccccctg acagagcggc agcaggccct cttccagcgg
     4021 ctgcgggagg agcgcgccca cctggcccgc ctccggggcg tgccggcttt tgtgattttc
     4081 tccgacaaga ccctgcggga gatggcggct gcccagccac gggatctgcg tcagctccag
     4141 aacgtgggcg gcgtgggcag cttcaaggcg gagcagtacg gagctcagtt cctggagatc
     4201 atctcggatt ttcaggagac agcggcaata taagagcctg ccccagacag atggtgtccg
     4261 gggcaggctc ttatatacat attttacttc ggagcgtggg ggccgcagcg agtgtggtgt
     4321 ccgccgcacc cgccgcaggt gccgcagtcc ccattgcagt ggccgcccga cttgtgggac
     4381 tgccacaggg agcggaccgc gagggcgacc acgcccgcaa ggatcaggga gacgatgatg
     4441 gtaggcagca gctgggacat atctgaccct ccttgtcgat cacagattat ttcttgccgg
     4501 cagggatgtc ggtcaggtga cggacctcct cgctgggctg gtagcccctg cgcaccagca
     4561 ggaagatgat gccggccagc agggcgatgg cgatgacggt gaagacgctg aaggcggcct
     4621 cgccggtgaa cagaccgccg atctgataga cgatcatggc agtcacatag gcgaacaggc
     4681 acatgtagcc gatggcgccc agagtccact ttgtgttgtg catctcccgc ttgatggcgc
     4741 ccatggcagc gaagcagggg gcgcacagca ggttgaagat catgaaggag taggcggaga
     4801 tggcgccgaa gtcagccccc accatagccc agatggggct ggagtcctcc atcagatcga
     4861 tgtcgccggc gtagtggtac agcacaccga aggtgttgac cacctcctcc ttggcgatga
     4921 ggccggtgaa ggtagccact gcggccttcc aggccatgga accggtccag cccagggggt
     4981 agaagatcca ggcgatggca ttgccgatga cggccagcag ggagtcattg ttgtcctcca
     5041 cggcctggaa cacgccgtcc acaaaaccgt agccctggag gaaccacagg atgatggagg
     5101 agatcaggat gacggtgccg gcccgcttga tgaaggacca gccacgctcc caggtggccc
     5161 gcagcacgtt gccggcggag ggggcgtggt aggcgggcag ctccatgaca aagggggcgg
     5221 gctcgccggc aaaggccttg aatttcttca gcatgatgcc ggagacgatg accgccgcca
     5281 cgccaatgaa gtaggcggag gtagccaccc aggcggagtt gccaaagaca gcgccggcga
     5341 acaggccgat gatgggcatc ttggcgccgc agggaatgaa gccggtggtc atgatggtga
     5401 ttttgcggtc acggtcctgc tcgatggtgc gggatgccat gatcccgggc acgccgcagc
     5461 cggtagccac cagcatgggg atgaagctct tgccggacag gccgaagcgg cggaagatcc
     5521 ggtccatgat gaaggcgatg cgggccatgt agcccacatc ctccaggaca gccagcagga
     5581 cgaacaggac cagcatctgg ggcacgaagc ccagcacggc gcccacgccg gccacgatgc
     5641 cgtcctggat caggccgtac agccagtcgg ccaccacagg ctcgccgctg gcgttgagca
     5701 ggaagctgtc aaagaagccg ggcacccact caccgaagat ggtgtcgttg gtgaagtcag
     5761 tggcggcggt gccgatggag atggcccagc cgcccatggc gatggcatag atcagaaaca
     5821 tcactgccac gaagatgggc agggccagga cacggttggt cacgatccgg tcgatcttgt
     5881 cggaggtggt caggctgtgc ttgggggcct tcttttttac tgccctgttg accacagagt
     5941 tgatgtacgc atagcgctgg ttggtgatga tgctctcagc gtcgtcctcc agctcgttct
     6001 cacagtcggc gatgtgggcc tccaggtggg cctccaggtc tttggtcagg gtcagggact
     6061 ccagcacctt ctcgtcccgc tcgaacacct tgatggcata ccagcggaga tactggtcgt
     6121 ccaccatgcc ctgaatggac tcctcaatgt gggcgatggc gtgctctacg ctgccagtga
     6181 acac
//
LOCUS       DBMDSJ010000051         6135 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3692_length_6135_cov_1.401724, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000051 DBMDSJ010000000
VERSION     DBMDSJ010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3692_length_6135_cov_1.401724"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="ACZPAQ_02120"
     CDS             <1..181
                     /locus_tag="ACZPAQ_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR0902805.1"
                     /translation="NYSLACQMDPGNMEYRQALAMMQQGGQAYRPYGHSGGMDGLDCC
                     TSLLCLNCLCGGGHC"
     gene            183..707
                     /locus_tag="ACZPAQ_02125"
     CDS             183..707
                     /locus_tag="ACZPAQ_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902806.1"
                     /translation="MASFHTTSRRTAVAGVLAAGSLAVLWLACMVPSGRIGLTGAAGL
                     FPVAAVLAAGRAAGYLCWAASALLGLFLLPDKGVALLYLIFLGLYPVVKNNIEGLRRL
                     PLEWLCKLACFNAALSLFWFALRALFLPDPPVWLGERTWLLYLAGNLIFVLYDVGLSR
                     LIALVMARMPGRRR"
     gene            736..1614
                     /locus_tag="ACZPAQ_02130"
     CDS             736..1614
                     /locus_tag="ACZPAQ_02130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR0902807.1"
                     /translation="MVKSMTGYGRAEQSLNGRTITVELRSVNNRYLDCSIRIPRLYLF
                     AEDGLKSQVQSSISRGKVDVFVTIDSAGAAGVKVSINRPVADGYCAALRELAQSYGLS
                     DDISVSLLSRFPDVLLVEKEEEDAEQVAQDISAVMEQALSDFDRMRSREGQKLSDDIL
                     TRADTIERLVTLVEERSPQTVEEYRARLEAKMNEVLANTQLDPARILTEAAIFADKVA
                     VDEETVRLRSHLGQLRHMLAQGGATGRKLDFLIQEFNREANTIGSKCSDIEIARYVVD
                     IKAEIEKIREQVQNIE"
     gene            1628..1894
                     /gene="remA"
                     /locus_tag="ACZPAQ_02135"
     CDS             1628..1894
                     /gene="remA"
                     /locus_tag="ACZPAQ_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm regulator RemA"
                     /protein_id="HIR0902808.1"
                     /translation="MKLINIGFGNMISAGRLVAIVSPDSAPIKRMVQEARDRGTLIDA
                     TYGRRTRSVLIMDNDHLVLSALQPDTVASRLESREAPEAEEDEE"
     gene            1891..2508
                     /gene="gmk"
                     /locus_tag="ACZPAQ_02140"
     CDS             1891..2508
                     /gene="gmk"
                     /locus_tag="ACZPAQ_02140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR0902809.1"
                     /translation="MTKKKEKGQLIVLSGPSGVGKSTVIAELLGQRKGIYFSVSYTTR
                     QPRVGEADGINYNFVSRTEFERMIADQELLEYAEYVNNYYGTSLKMIQEKLNAGIDVL
                     LDIEVQGAAKVRARCPEAIFIFIIPPSFEELSRRLHNRNTDNEDVIAGRLEKAKIEFR
                     EIPTYDYLVINDKVSNAVEEIEAILVANECRVDNRRNILKGEFSL"
     gene            2505..2699
                     /gene="rpoZ"
                     /locus_tag="ACZPAQ_02145"
     CDS             2505..2699
                     /gene="rpoZ"
                     /locus_tag="ACZPAQ_02145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR0902810.1"
                     /translation="MMLYPAMRDLLAKVPSRYQLVNVVACRARQIASEAETAGVPLDD
                     KPVSIAIREVAEGQLDVSES"
     gene            2783..5242
                     /gene="priA"
                     /locus_tag="ACZPAQ_02150"
     CDS             2783..5242
                     /gene="priA"
                     /locus_tag="ACZPAQ_02150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR0902811.1"
                     /translation="MVAKVAVSKAIYAIDKPYDYLVPPELEGQVLPGMRVAVPFGSGN
                     RGSDGIVLSLERQEENTPTLKPVLSCLDDAPVLDEKGIQLALWMRERYFCTVSDAVKA
                     MLPAGMYFALRDSVTLVPGLEPEQIWDLLSDAPAAARLAEMLCCWGGSGDMTQIHTAF
                     GAKDPTPAIRVLLDRKIAVLETSAQRAVGDKTERLAVLNMPAEEAMELVASRRKRAPL
                     RYAVTELLCTLGAASTKELCYFTGASPATLRSLEKSGILTLEKHEVLRRVRVEDVEPA
                     GPVELNQEQEAAFRGLDRFCLRGEAGAALLYGVTGSGKTQIYIRLIQEVLARGQTAMV
                     LVPEISLTPQLLRVFASHFGELVAVLHSSLRSGERYDEWKRVRSGAARVVLGTRSAVF
                     APLDHIGLIVLDEEQESSYKSENAPRYHARDVAKYRCVQHRAVLLLGSATPAVESMYQ
                     AREGRYRLFTLSQRFNQQAMPQVRIVDMKRELRAGNCTAISAELRRELACNLERGEQS
                     ILFLNRRGSSRMVSCGECGQVPECPRCSVRLTYHSANGRLMCHYCGYSEPLPPACPSC
                     GGRLNFIGVGTQRVQEELEALFPGIEILRMDADTISAAHPHEELLSRFQKQRVPILVG
                     TQMVAKGLDFDNVTLVGVVDADLSLYAADFRAGERTFSLITQVVGRAGRGEKRGRAVI
                     QTYTPDNDVIRFAAGQDYDSFYEQEIQLRRLRDEPPFRDILVLTASGPDEAGVLRVCT
                     RLRQFLERELPKLPDQSFRLLGPAPAVIARVNNRYRYRLTLVGQDSRPLRSLIAALLR
                     AVRQDRESRGTSVFADLNPMD"
     gene            5265..5756
                     /gene="def"
                     /locus_tag="ACZPAQ_02155"
     CDS             5265..5756
                     /gene="def"
                     /locus_tag="ACZPAQ_02155"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR0902812.1"
                     /translation="MALREIIKKGDPVLEKHCHPVTRFDQKLWDLLDDMRETLAQANG
                     LGLAAPQVGILRRAVLVVNDQEEMLELINPEIIASEGEEDGLEGCLSVPGYWGYVKRP
                     AWVKVRAHDRNGNEFETEGTGMTARCFCHELAHLDGQLYTDLADRIYTTEELDAMLEE
                     QGK"
     gene            5753..>6135
                     /locus_tag="ACZPAQ_02160"
     CDS             5753..>6135
                     /locus_tag="ACZPAQ_02160"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR0902813.1"
                     /translation="MTILFMGTPDFAVPSLEALIAAGHTICGVFTQPDRPKNRGMKLQ
                     APPVKRIALAHDIPVFQPVTLKDGTALEEIRQLSPELIVVAAYGRLLPREILELPAKG
                     CINVHSSLLPKYRGAAPINWAVLNG"
BASE COUNT         1154 a         1842 c         2038 g         1101 t
ORIGIN      
        1 gaactactcc ctggcctgcc agatggaccc agggaacatg gagtaccgcc aggccctggc
       61 catgatgcag caggggggac aggcataccg cccctacgga cacagcggag ggatggacgg
      121 tctggactgc tgcaccagcc tgctgtgcct gaactgcctg tgcggagggg ggcactgctg
      181 agatggcctc ttttcacacc accagccgcc gcaccgcggt tgccggggtc ctggcagctg
      241 ggagcctggc cgtgctctgg ctggcctgta tggttccgtc agggcgcatc ggcctgaccg
      301 gcgcggccgg tctgtttccg gtggccgcgg tgctggccgc cggacgggcg gcgggctatc
      361 tgtgctgggc cgcatccgcc ctgctggggc tgttcctgct gccggacaag ggggtcgctc
      421 tgctctacct gatcttcttg gggctctacc cggtagtcaa aaacaacatc gagggcctgc
      481 ggcgcctgcc cctggagtgg ctgtgcaagc tggcctgctt caacgcggcc ctgtctctct
      541 tctggttcgc gctgcgggcg ctgttcctgc ccgatccccc tgtgtggctg ggggagagga
      601 cctggctgct ctatctggca gggaatctga tcttcgtcct ctatgacgtg ggcctgtccc
      661 gcctgatcgc gctggtgatg gcccggatgc cggggcgccg ccgctgaaat caaaataagg
      721 aagtgaaccg caccaatggt gaaaagcatg accggctacg gccgggcgga gcagagcctg
      781 aacgggcgta ccatcacggt ggagctccgt tccgtcaata accgatatct ggattgctct
      841 atccgcattc ccaggctcta tctgtttgcg gaggatgggc tgaagtctca ggtccagagt
      901 tccatttcca ggggaaaggt ggatgtcttc gtcacaatag acagcgcagg cgccgccggc
      961 gtgaaggtct ccatcaatcg gccggtggcg gacggatact gcgctgccct gcgggagttg
     1021 gcccagagct atgggctgtc cgatgacatc tccgtctccc tgctctcccg atttcccgat
     1081 gtgctgctgg tggagaagga agaggaggat gcggagcagg tcgcccagga catcagtgcc
     1141 gtcatggaac aggcgctgtc cgactttgac cggatgcgca gccgggaggg ccagaagcta
     1201 tctgatgaca tcctgacccg ggcggacacc attgaacgcc tggtcaccct ggtggaggag
     1261 agatcccccc agaccgtgga ggaataccgg gctcgtctgg aggctaaaat gaatgaggtg
     1321 ctggccaaca cccagctgga cccggcccgc atcctcacag aagcggccat ctttgctgac
     1381 aaggtggcgg tggacgagga gacggtgcgc ctgcgcagcc acctggggca gctgcggcac
     1441 atgctggctc agggcggagc caccgggcgc aagctggact ttctgatcca ggagttcaac
     1501 cgggaggcca acaccatcgg ctcgaagtgc agcgacatcg agatcgcccg atatgtggtg
     1561 gatatcaagg ctgaaatcga gaagatccgg gagcaggtcc agaacatcga atgagggggc
     1621 gggacatttg aagctcatca acatcggatt tggcaatatg atctccgccg gccggctggt
     1681 ggctatcgtg tcgccggatt cggcccccat caagcgcatg gtgcaggagg cccgggaccg
     1741 gggcacgctc atcgatgcca cctatggccg ccggacccgg tcggtgctca tcatggacaa
     1801 cgaccatctg gttctctcag cgcttcagcc ggacactgtg gccagccgtc tggagagccg
     1861 ggaggcccct gaagcagagg aggatgagga atgaccaaaa agaaagagaa gggacagctc
     1921 atcgttttgt ccggtccatc cggtgtgggt aagagcaccg tgatcgcaga gcttttgggc
     1981 cagcggaagg ggatctattt ctctgtgtcc tacaccaccc gccagccccg ggtgggagag
     2041 gcagacggga tcaactacaa cttcgtctcc cggacggagt tcgagaggat gatcgcggac
     2101 caggagctgc tggaatacgc ggaatatgtg aacaactact acggcacctc cctgaagatg
     2161 atccaggaaa aactgaacgc cggcatcgac gttctgctgg acatcgaggt gcagggggcg
     2221 gcaaaggttc gggcccgatg ccctgaagcc atctttatct ttatcatccc gccctccttt
     2281 gaggaactct cccgccgtct ccacaaccgg aacacggaca acgaggacgt gattgccggc
     2341 cggctggaga aggcgaagat agagttccgg gagatcccga cctacgacta tctggtgatc
     2401 aacgacaagg tctccaacgc agtggaggaa atcgaggcca tcctggtggc caatgagtgc
     2461 cgggtggaca accgcagaaa cattttgaaa ggggaatttt cgttatgatg ctttatcctg
     2521 ctatgagaga tctgctggct aaggtgccca gccgctatca gctggtcaat gtggtggcct
     2581 gccgcgcccg gcagattgcc agcgaggcgg agaccgccgg cgtccctctg gacgacaaac
     2641 cggtgagcat tgccatccgc gaagtagcgg agggtcagct ggacgtgtct gaatcgtaag
     2701 caggcggcga ggagcaagcg gagggaggct tcccacgctt gctttgcctt gtacggaaga
     2761 ggaggggatc ggtatcggga gagtggtggc caaggtggcg gtatccaaag cgatctacgc
     2821 catcgacaag ccctatgact atctggtccc tccggagctg gagggacagg tactgcccgg
     2881 gatgcgggtg gccgtccctt tcgggagcgg aaaccgtggg tcggatggca tcgtgctctc
     2941 cctggaacgg caggaggaga acacacccac actgaagccg gtgctgtcct gtctggacga
     3001 cgctcccgtc ctggatgaaa aggggatcca gctggccctg tggatgcggg agcggtactt
     3061 ctgcactgtc agcgacgcgg tgaaggccat gctcccggcg gggatgtact ttgccctgcg
     3121 ggacagcgtg accttagtcc ccgggctgga gccggaacag atctgggatc tgctctcgga
     3181 cgctccagct gccgccaggc tggcggagat gctctgctgc tgggggggga gcggggacat
     3241 gacccagatc cacaccgcct ttggcgccaa ggaccccacc cccgccatcc gggtccttct
     3301 ggaccggaag atcgcggtac tggagaccag cgcccagcgg gcggtggggg acaagacaga
     3361 gcggctggcc gtgctgaata tgccggcaga ggaggccatg gagctggtcg cctccagacg
     3421 gaagcgagct cccctgcgct acgcggtgac ggagctcctc tgcactctgg gggcggcctc
     3481 caccaaggag ctttgctatt ttaccggcgc ctctcccgcc acgctgcgat ctctggaaaa
     3541 gagcggcatc ctcaccctgg agaagcatga ggtgctccgg agggtccggg tagaggacgt
     3601 ggagcccgcc ggaccggtgg aattaaatca ggagcaggag gcggccttcc gcggcctgga
     3661 ccggttctgc ctccgggggg aggcgggcgc cgccctgctg tacggcgtca ccggcagcgg
     3721 aaagacccaa atctatatcc gcctgatcca ggaggtgctg gccagggggc agaccgccat
     3781 ggtactggtc ccggagatat ctctgacgcc ccagctgctg cgggtgttcg cctcccactt
     3841 tggggagctg gtggcggtgc tccacagctc cctgcgcagc ggagagcggt acgacgagtg
     3901 gaagcgggtc cgttccggcg cggcccgggt ggtgctgggc acccgttccg ctgtttttgc
     3961 ccctctggac cacatcgggc tgatcgtgct ggacgaggag caggagagca gctataaatc
     4021 agagaacgcc ccccgctacc atgccaggga cgtggcaaaa taccgctgtg tccagcatag
     4081 agcggtgctc cttctgggct cagccacccc ggcggtggag tccatgtacc aggccaggga
     4141 ggggcgctac cgtctgttta ccctgagcca acgcttcaac caacaagcca tgccgcaggt
     4201 ccgcattgtg gatatgaagc gggagctgcg ggcggggaac tgcaccgcca tcagtgcgga
     4261 gctgcgccgg gagctggcct gcaacctgga acgcggggag cagagcatcc tgttcctgaa
     4321 ccgccggggc agcagccgca tggtctcctg cggggaatgc ggacaggtcc cggagtgccc
     4381 acgatgctcg gtgcggctga cctaccactc cgccaacgga aggctgatgt gccattactg
     4441 cggctattct gagccccttc ctcctgcgtg tccttcctgc ggcggcaggc tgaacttcat
     4501 cggtgtgggg acccagcggg tccaggagga actggaggca ctgtttcccg ggatcgagat
     4561 cctccgcatg gatgcggata ccatctccgc cgcccacccc cacgaggagc ttctatcccg
     4621 atttcagaaa cagcgcgtcc ccatcctggt tgggacccag atggtggcca aagggctgga
     4681 ctttgacaat gtgaccctgg tgggggtggt ggacgcagat ctgtccctct atgccgccga
     4741 cttccgcgcc ggggagcgca ccttctctct gatcacccaa gtggtgggtc gggccggccg
     4801 cggggagaag cgggggagag cggtgatcca gacctacacc ccggacaacg acgtcatccg
     4861 tttcgccgcc ggccaggact atgacagctt ctatgagcag gagatccagc tgcgccgcct
     4921 gcgggacgag ccgccctttc gggacatctt ggtgctgacc gcctccggcc ccgacgaggc
     4981 gggagtgctc cgggtgtgca cccggctgag gcagtttttg gagagagagc tccccaaact
     5041 gcccgatcag tccttccgcc tgctgggtcc cgcgccggca gtcatcgcca gagtcaacaa
     5101 ccggtatcga taccgcctta cactggtcgg acaggacagc cgccctctcc gttccctgat
     5161 cgccgccctc ctccgggcgg tgcggcagga ccgggagagc cgggggactt ctgtatttgc
     5221 cgacctgaat cccatggatt gattatgcaa aggagaaatg tttcatggcg cttcgagaga
     5281 tcatcaaaaa gggggaccct gtcctcgaga aacactgcca tccggtcaca cgcttcgatc
     5341 aaaagctgtg ggatctgctg gacgacatgc gggagaccct ggcccaggcc aacgggctgg
     5401 gcctggccgc gccccaggtg ggcatcctgc gcagagcggt cctggtggtc aacgaccagg
     5461 aggagatgct ggagctgatc aaccctgaga tcattgccag cgagggggag gaggacggcc
     5521 tggagggctg cctctccgtg cccggttact ggggctatgt gaagcgtccc gcatgggtga
     5581 aggtccgggc ccacgaccgg aacggaaatg agtttgagac tgaggggaca ggcatgaccg
     5641 cccggtgctt ctgccacgag ctggcccacc tggacggaca gctctacacg gatctggccg
     5701 accggatcta caccaccgag gagctggacg ccatgctgga ggagcagggg aaatgacgat
     5761 tctctttatg ggtaccccgg atttcgctgt cccgtcccta gaggcgctga ttgccgcagg
     5821 acacaccatc tgcggcgtat tcacccagcc ggaccggccg aaaaacaggg gaatgaagct
     5881 ccaggcacca cctgtaaagc ggatagccct tgcacatgat attcctgttt tccagcccgt
     5941 tactctcaag gacggcactg ccctggagga gatccgccag ctgtcccctg agctgatcgt
     6001 agtggcggcc tacggccgtc tcctgccccg ggaaattttg gagctgccgg ccaaggggtg
     6061 catcaatgtg cactcctccc tgctccccaa gtaccggggg gctgccccca tcaattgggc
     6121 ggtgctcaac gggga
//
LOCUS       DBMDSJ010000052         6131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3694_length_6131_cov_1.758706, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000052 DBMDSJ010000000
VERSION     DBMDSJ010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3694_length_6131_cov_1.758706"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="ACZPAQ_02165"
     CDS             complement(<1..793)
                     /locus_tag="ACZPAQ_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0902814.1"
                     /translation="MPEIKLEHVTKRWGKFYAVDDLDLVIQDNAFVTLLGPSGCGKTT
                     TLRMIAGLETPTSGRITIGDQVVFDSALGINIPANKRKVGFLFQNYALWPNMTVYENI
                     SFGLKNIKEPLPKVDFEAKNAARLAEILEQPAEVVRVLEECRDKDGRLDQKKAQLKLV
                     DTFTISIYTAKQLLSYRLEQSPDLSGEIASLRGRVEAARQSAAAAGGELDGEFRLVKG
                     GQVITETRKLTREEIDLTVRRVARIVKIGMFMDRYPAELSGGQQQR"
     gene            complement(797..2626)
                     /locus_tag="ACZPAQ_02170"
     CDS             complement(797..2626)
                     /locus_tag="ACZPAQ_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0902815.1"
                     /translation="MNQTTTPGAARSAILWNRLRTFLSKPHNVILLLMGIVVTITTIA
                     PIVAIVEDTFKIHPGTIDAHLTGQTAGYTLVNYIDLFTSTLAKTNLWTPLLNTIYLAV
                     GTCLVSILFGGVFAFLVTRTNLAWRKYLSSIFIFPYIMPQWTLAVVWQNLFNSNAVTG
                     TSNGLLAATAGIQMPLWWCKGLFPSLVVLGLHYAPFAYILIGGIFRNMDANLEEAATI
                     LDTPKWKTMCKITLPMVKPAILSTILLVFGSSMGSYPVPHYLGLTTLSIKYVSMNSKY
                     TGEASILAIIMMIFGVAIMLLNQMSLHSRKNYTTVTGKSGQISKINLGRAGKYIIALV
                     LVVVTFFTSIFPIVSFAFETFLPNPGDYSFLYTGDPDNLTTKWWVTDENITENGMYGQ
                     KGILHNDTIWNAFKGTLLVSVACALLAGTIGTLIGYAVSKNRRSKAASYVNSMAFLPY
                     LMPSIAVGAAFFVLFSNEHVNLFNTYTLLIIVGTIKYIPFASRSSLNSMLQLSGEIEE
                     AAIIQDVPWVKRMTRIIIPIQKSSIISGYMLPFMTCLRELSLFMLLCTQGFILSTTLD
                     YFDEMGLYAFSSGINLILIITILVFNTVVNKVTGASLDEGIGG"
     gene            complement(2758..4170)
                     /locus_tag="ACZPAQ_02175"
     CDS             complement(2758..4170)
                     /locus_tag="ACZPAQ_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902816.1"
                     /translation="MKRTISLLLACALSFSLAACGGGGGTQGGGSSAGTSAGSSAPAG
                     SEPQPQPSADLTPTQQIIQEAQGMTLEELAQKAIEESNGKTFLGVGNSSRGKSALPLF
                     IEYLQSIDPSYTMEFEWQQPKNNKIFDQLTADSLKDTGTFAMTLIQDGNQIESKMVQT
                     GILDTFIPKDWAEANGTTPEEYQGYLPLQTLNKIFMYNNTGSKSYDNCWDFVAEGEHG
                     LFMDIDSEIVGKNFLYMLTRDDYAAMLKEAFDALSAEEQAYFQPTINEMASEAESLGL
                     GENGKYALAWIKLWVGSYNAQTDDGPICNTLVDQSATDQFGLIVYSKLRSVEESASVS
                     KNNITVAAYNDGYTGMGGFGYCHYLFVTDNSPLPWTACAFIAYMTCTADGFSAWGKDI
                     GGYSSNPEVAAENEAIYHHETGGMAEDGTTVEYAALNDHGYDWWTNEGKLVLEDPEYC
                     ASVSFTVGSWIEMLDKYSAG"
     gene            4395..5420
                     /locus_tag="ACZPAQ_02180"
     CDS             4395..5420
                     /locus_tag="ACZPAQ_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0902817.1"
                     /translation="MRGRLAALALTLGLLLTGCAAGERASASEFAPEEGERLVVYTSH
                     KEEVYWPIIQEFEARTGIWVEVEAGGTSELLARLRREADAPAADVMFGGGVDSLEAYQ
                     DCFSPYFCAGWEGLDPAMRSEEGLWTPFSALPVVIIYNTKLVVPGQITGWRDLFSPAW
                     QGRIAFCDPSVSGSSFTGLITLLRAVDRDTDETLMRFAVSLDGRQLDSSGAVLDSVAG
                     GTDLVGITLEETALKRAAAGEDIALIYPDDGTSCIPDGGALVQGAPHPENARKFLDFI
                     AGNDVQSRLETEFSRRPVREGVESGGVLRPLEELVLVDYDLAWTVEHHDSILMSWAFY
                     LGGGEEP"
     gene            5417..>6131
                     /locus_tag="ACZPAQ_02185"
     CDS             5417..>6131
                     /locus_tag="ACZPAQ_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902818.1"
                     /translation="MKKRHSFRRRLFAGFLLASLLPLLLCSAMLLQIFRLRLDSQLEE
                     ESREQLDHVISSLDGLYRGFQQTALALQASPYVAPALSRGQRDSTTVYSELYSATEGS
                     RLSAQFDLYDRDGVWMYSTGSGPVEPVMPTQWGVLYAARQSVGLAFTQVDGGEGNSAP
                     LFRGAARIAGEAGEDAGYLVITLTRADFQRLLEGTYGGQSDLLLLDRYWRPVYASQTA
                     LAGRLAVQLREQLLAGEELG"
BASE COUNT         1081 a         1696 c         2157 g         1197 t
ORIGIN      
        1 cccgctgctg ctggccgccg gagagctctg cggggtagcg gtccataaac atgccgatct
       61 tcacgatgcg ggccacccgg cggacggtga ggtcgatctc ctcccgggtg agcttgcggg
      121 tctcggtgat cacctggccc ccttttacca ggcggaactc cccgtccagc tcgccgcccg
      181 ccgcagcggc ggactgcctg gcggcctcca ccctgccccg gagggaggcg atctctccag
      241 acaggtcggg ggactgctcc aggcggtagg agaggagctg cttggcggtg tagatggaga
      301 tggtgaatgt gtccaccagc ttcagctggg ccttcttctg gtccagcctg ccgtccttgt
      361 cccggcactc ctccagcacc cggaccacct cggcgggctg ctccaggatc tccgctaaac
      421 gggcggcgtt cttggcttcg aagtcgacct tgggcagggg ctccttgatg tttttcaggc
      481 cgaaggagat gttctcatag acagtcatat tgggccacag ggcgtagttt tgaaagagaa
      541 agcccacttt gcgcttgttg gccgggatgt tgatgcccag ggcgctgtca aagaccacct
      601 ggtcgccgat ggtgatgcgg ccgctggtgg gggtctccag accggcgatc atccgcaggg
      661 tggtggtctt gccgcagccg gaggggccca gcagagtgac gaaggcgttg tcctggatga
      721 ccagatccag gtcatccacg gcgtagaact tgccccagcg cttggtcaca tgttctaatt
      781 tgatctcagg catggtttaa cctcctattc cctcgtccag actggctccg gtgaccttgt
      841 tgaccacggt gttgaacacc aggatggtga tgatcaggat caggttgatg ccgctggaga
      901 aggcgtatag acccatctcg tcgaagtagt ccagggtggt ggacaggatg aagccctggg
      961 tgcacagcag catgaacagg gacagctccc gcaggcaggt catgaagggg agcatatagc
     1021 cgctgatgat ggaggacttc tggatgggga tgatgatgcg ggtcatccgc ttgacccagg
     1081 gcacatcctg gatgatagcg gcctcctcga tctcgccgga cagctggagc atggagttga
     1141 gggagctgcg gctggcgaag gggatgtact tgatggtgcc tacgatgatg agcagagtat
     1201 aggtattgaa gaggttgacg tgctcattgg aaaacaggac gaagaaggcc gcccccacgg
     1261 cgatggaggg catgaggtag ggaaggaagg ccatggagtt cacatagctg gccgccttac
     1321 ttcgccggtt cttggacacg gcgtagccga tgagggtgcc gatggtgccc gccagcaggg
     1381 cacaggccac gctgaccagc agggtgccct tgaatgcgtt ccagatagtg tcgttgtgga
     1441 ggatgccttt ctgtccgtac atgccgtttt cggtgatgtt ctcatcggtg acccaccact
     1501 tggtggtcag gttgtccggg tccccggtgt ataggaagga gtagtccccc ggattgggga
     1561 ggaaggtctc gaaggcgaag gagacgatgg ggaagatgct ggtgaagaag gtgaccacca
     1621 ccagtaccag ggcgataatg tacttgcccg cccggcccag gttgatcttg gagatctgcc
     1681 ccgacttgcc ggtgacggtg gtgtagttct ttcggctgtg gagggacatc tggttgagga
     1741 gcatgatggc cacgccaaag atcatcatga tgatggccag gatgctggcc tcgccggtgt
     1801 acttggagtt catggacacg tacttgatgg acagagtggt caggcccagg tagtggggca
     1861 cggggtagga gcccatggag ctgccgaaga ccagcaggat ggtggacaga atggccggct
     1921 tcaccatagg cagggtgatc ttgcacatgg tcttccactt gggggtgtcc agaatggtgg
     1981 ctgcctcctc caggttggcg tccatgttgc ggaagatgcc cccgatgagg atataggcga
     2041 agggggcgta gtgcaggccc agcaccacca ggctgggaaa gaggcccttg caccaccaca
     2101 ggggcatctg gatgcctgcg gtagcggcca gcaggccgtt ggaggtgccg gtgacggcgt
     2161 tggagttgaa caggttctgc cacaccacag ccagggtcca ttggggcatg atgtagggga
     2221 agatgaagat ggagctgagg tatttccgcc aggccaggtt ggtgcgggtg accaggaagg
     2281 cgaacactcc gccgaagagg atggacacca agcaggtgcc cacggccaga tagatggtgt
     2341 tcagcagggg ggtccacagg ttggtcttgg ccaaggtgct ggtgaacagg tcgatgtagt
     2401 tcaccagggt atagccggcg gtctggccgg tgagatgggc atcgatggtg ccggggtgga
     2461 tcttgaaggt gtcctccaca atggccacga tgggggcgat ggtggtgatg gtcaccacga
     2521 tgcccatcag cagcaggatc acgttgtgag gcttggacag gaaggtcctg agcctgttcc
     2581 agaggatggc ggaccgtgcg gcgccggggg tcgtggtttg gttcatatgc ggcactcctc
     2641 tcatgcggga atggaggggc gcccttggag gcaactcggc tccaagggcg ccctgctctg
     2701 cgtttcgtca aaaccggcgc tcagcgccca gaggtccggt gacgccgggg atggggctca
     2761 gccggcgctg tacttgtcca gcatctcgat ccagctgccc acggtgaagg agacggaggc
     2821 gcagtactcg gggtcctcca ggaccagctt gccctcgttg gtccaccagt cgtagccgtg
     2881 gtcgttcagg gcggcgtact ccacggtggt gccgtcctcg gccatgccgc cggtctcgtg
     2941 gtgatagatg gcctcgtttt ccgcagccac ctcgggatta gaggagtagc cgccgatgtc
     3001 cttgccccag gcggagaagc cgtcggcggt gcaggtcatg taggcgatga aggcgcaggc
     3061 agtccagggc agagggctgt tgtcggtgac gaacagatag tggcagtagc cgaaaccgcc
     3121 cattccggtg tagccgtcgt tataggccgc cacggtgatg ttgttcttgg agacggaggc
     3181 ggactcctcc acgctgcgca gcttggagta gacgatcagg ccgaactggt cagtggcgga
     3241 ctggtccacc aaggtgttgc agatggggcc gtcgtcagtc tgggcattgt agctgcccac
     3301 ccacagcttg atccaggcca gggcgtattt gccgttctcc cccaggccca ggctctcggc
     3361 ctcggaggcc atctcgttga tggtgggctg gaagtaggcc tgttcctcgg cggacagggc
     3421 atcgaaggcc tccttcagca tggcggcgta gtcatcccgg gtgagcatat acaggaagtt
     3481 cttacccacg atttcagagt cgatgtccat aaacaggccg tgctcgccct cggccacgaa
     3541 atcccagcag ttgtcatagg atttggaacc ggtgttgttg tacatgaaga tcttgttcag
     3601 agtctgaagg ggcagatagc cctggtactc ctcgggggtg gtgccgttgg cctccgccca
     3661 gtccttgggg atgaaggtgt ccagaatgcc ggtctggacc atcttggact cgatctggtt
     3721 gccgtcctgg atcagggtca tggcaaaggt gccggtgtcc ttcagggagt cggcggtgag
     3781 ctgatcgaag atcttgttgt tcttgggctg ctgccactcg aactccatgg tgtagcttgg
     3841 gtcgatggac tgcagatact cgatgaacag gggcagggca ctcttgccgc ggctggagtt
     3901 gcccacgccc aggaaggtct tgccgttgga ctcctcgatg gccttctggg ccagttcctc
     3961 cagggtcatc ccctgggcct cctggatgat ctgctgggtg ggtgtcagat cggcggaggg
     4021 ctggggctgg ggttcgctgc cggcaggggc ggagctgccg gcgctggtcc ccgcagagct
     4081 gccgccgccc tgggtcccgc cgccaccgcc gcaggctgcc agggagaagc tgagggcaca
     4141 ggccaggagc aatgaaatgg tacgtttcat tttggttcct ccttcatcat ttttcataaa
     4201 attgaacctg atatcccagg tcctgtgtgc aattttattg tatcgcctcc gtgatggaaa
     4261 aaacagggga caaatctgcg cttttttgtt atcttctgca atttctgtgg aaattggatg
     4321 aaaagaggag ggggggtgtg atataattat gcaaaacacc aaactgagca caaaacaggc
     4381 gaggggggag cggaatgaga ggacggctgg cagcgctggc cctgacattg gggctgctcc
     4441 tgaccgggtg cgcggccggg gagagggcct ccgcatcgga gttcgccccg gaggaggggg
     4501 agcggctggt ggtgtacacc tcccacaagg aggaggtcta ctggcccatc atccaggagt
     4561 tcgaggcgcg caccggcatc tgggtggagg tggaggcggg gggcaccagc gagctgctgg
     4621 cccgcctgcg ccgggaggcg gacgccccgg cagcggatgt gatgttcggc ggcggcgtgg
     4681 acagcctgga ggcatatcag gactgcttct ccccctactt ctgtgccggt tgggaggggc
     4741 tggacccggc catgcgctcg gaggagggac tgtggactcc cttctccgcc ctgccggtgg
     4801 tgatcatcta caacaccaaa ctggtggtcc cggggcagat caccggctgg cgggatctgt
     4861 tctctccggc atggcagggg cgcatcgcct tctgcgatcc cagtgtgtcc ggctcctcct
     4921 tcaccgggct gatcaccctg ctccgggcgg tggacaggga tacggacgag accctgatgc
     4981 gctttgctgt cagcctggac ggccggcagc tggacagctc cggtgcggtg ctggactcgg
     5041 tggccggggg gacggacctg gtggggatca ccctggaaga gaccgccctg aagcgggcgg
     5101 cggcgggaga ggacatcgct ctgatctacc ccgatgatgg caccagctgc atcccggatg
     5161 gaggcgccct ggtgcaggga gcgccccacc cggagaacgc ccggaagttc ctagacttca
     5221 ttgcaggcaa cgatgtgcag agccggctgg agacggagtt ttccagacgc cccgtacggg
     5281 aaggggtgga gagcggcggc gtgctgcggc cgctggagga gctcgtcctg gtggactatg
     5341 acctggcctg gaccgtggag caccacgaca gcatcctcat gtcctgggct ttctatctgg
     5401 ggggagggga ggagccgtga aaaaacgcca ttccttccgc agaaggctgt tcgcggggtt
     5461 cctgctggcc tccctgctgc cgctgctgct gtgctcggcc atgctgctcc agatcttccg
     5521 cctgcggctg gacagccagt tggaggagga gtcccgggag cagctggatc acgtgataag
     5581 ctccctggac ggcctgtacc ggggctttca gcagacagcc ctggcccttc aggcaagccc
     5641 ctatgtggcc cctgcactct cccggggaca gcgggacagc actaccgtct atagcgagct
     5701 gtacagcgcc accgagggca gccgtctcag cgcccagttc gacctgtatg accgggacgg
     5761 ggtctggatg tactccaccg gcagcggccc ggtggagccg gtgatgccca cccagtgggg
     5821 cgttctctac gccgcccggc agagcgtcgg gctggccttc acacaggtgg acggcgggga
     5881 ggggaacagc gcgcccctgt ttcggggggc ggcccggatc gccggtgagg cgggggagga
     5941 cgcgggctat ctggtcatca ccctgacccg ggcggacttc cagcgactgc tggaggggac
     6001 ctacggcgga cagagcgacc tgctgctcct cgaccggtac tggcggcccg tctacgccag
     6061 ccagaccgcc ctggccggcc gcctggctgt ccagctgcgg gagcagctgc tggcggggga
     6121 ggagctgggc c
//
LOCUS       DBMDSJ010000053         6103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3713_length_6103_cov_1.734755, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000053 DBMDSJ010000000
VERSION     DBMDSJ010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3713_length_6103_cov_1.734755"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="ACZPAQ_02190"
     CDS             complement(<1..452)
                     /locus_tag="ACZPAQ_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR0902819.1"
                     /translation="MEYANTFSLDFPVELWRPFAQDRPSVRCSSGYLIYLQGTEATCF
                     YYLKKGKVKSFIQSEDGAERTLNLYQQGSIFGEASFFDELPRVSSAVALTPCEIVPID
                     REQVTAEFTRNPGMAMAMLKYLARTVRLLSAHVDDMAFRPAEWRVAQYL"
     gene            573..1829
                     /gene="leuC"
                     /locus_tag="ACZPAQ_02195"
     CDS             573..1829
                     /gene="leuC"
                     /locus_tag="ACZPAQ_02195"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR0902820.1"
                     /translation="MGMTMTQKILAKAAGLSSVQPGQLIEARLNLVLGNDITTPVAIT
                     EFEKAGFTQVFDKDRIAIVLDHYTPCKDIKSAQLCKQAREFAHKHSITHFYDVGQVGV
                     EHALIPEKGLAAPGEVIVGADSHTCTYGALGAFSTGIGSTDMAAGMATGLLWFKVPSA
                     IKVTLKGKLQPCVSGKDVILHLIGEIGVDGALYQSLEFAGEGVSSLSMDDRFTISNMA
                     IEAGGKNGIFPVDEKTMEYINGRVGRPCEAFEADPDAVYTREVVIDLDKLEPTVSMPH
                     LPENTKVVSEVAGLHIDQVVIGSCTNGRIQDLREAAAILKGKKVAENVRCIVIPATQE
                     ITLQAMKEGLLEIFIASGCAVSAPTCGPCLGGHMGVMAEGERCVSTTNRNFVGRMGHV
                     QSEVILASPAVAAASAVAGCVADPRI"
     gene            1904..2389
                     /locus_tag="ACZPAQ_02200"
     CDS             1904..2389
                     /locus_tag="ACZPAQ_02200"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR0902821.1"
                     /translation="MKVYKYGANVDTDVIIPARHLNDPSPAALASHCMEDIDKDFAST
                     VQPGDIIVAGPNFGCGSSREHAPLAIKSCGVKCVIAPSFARIFYRNAINIGFPIMECA
                     QAAEEIQAGDQVEVDFATGVITDETTGKTYQAAPFPEFIDGIIKSGGLLNSLKDRGVA
                     K"
     gene            2386..3462
                     /gene="leuB"
                     /locus_tag="ACZPAQ_02205"
     CDS             2386..3462
                     /gene="leuB"
                     /locus_tag="ACZPAQ_02205"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR0902822.1"
                     /translation="MNYKIALIRGDGIGPEVVGEAVQVMEAVGKKFGHSFTFEDILMG
                     GCATDAVGVSYPEGTAEKCRACDAVLLGAVGGPKWGSDKPAEQRPETALLAIRKDLGL
                     YANLRPAALRPAMAGACPLKKETAEKGIDLMMVRELTGGIYFGKRDRYQTEDRGEEAT
                     DLMAYSEQEIERIGRRAFELARLRRNKVSSVDKANVLETSRLWRKVMHRLAEEYSDVS
                     YEDVLVDNCAMQFVRDPGQFDVVVTENMFGDILSDEASMVTGSIGLLPSASIGDAAPG
                     LYEPIHGSAPDIAGQDKANPIATILSVAMMFRYSFHLAAEAQAIENAVDAVLAEGWRT
                     ADIAEPGVIPIGTREMGRRIREKL"
     gene            3734..4933
                     /locus_tag="ACZPAQ_02210"
     CDS             3734..4933
                     /locus_tag="ACZPAQ_02210"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0902823.1"
                     /translation="MKELSKIALAVEPSSTMAIDTLFKQMKAEGQDVIGFGAGEPDFN
                     TPDYIKEAGIAAIEHNETRYTPSSGTVELRKAICQRLKADCGVEYTPQQISVSNGAKP
                     CVYVALRALINPGDEVILPSPYWVSYSELIKMTGGVPVIVETREADHFKLTAQELRDA
                     ITPKTKAMILNNPSNPTGMMYSRKELEEIAKVCVEQDIYVICDEIYYHLVYDGEEFVS
                     LASISPEIKERTLLVNGVSKSYAMTGWRIGYVAANDQVSKVIANYLSHCTGSPSSISQ
                     KAAVTALTASQESVEVMRKAFERRRDYMVERMNSIEGVSCIKPHGAFYVMMNIEKLIG
                     QELHGVVIRDADDFGNLFLKYGKVAVVPGTGFGAPTFVRWSYATSIENIKEGLNRLER
                     FLKGETI"
     gene            5115..>6103
                     /locus_tag="ACZPAQ_02215"
     CDS             5115..>6103
                     /locus_tag="ACZPAQ_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR0902824.1"
                     /translation="MPTNAYESPLSSRYASDEMLYLFSADKKFSTWRRLWVALARAEM
                     ELGLPVTQEQVDELEAHITDIDYEKAAQWERKLRHDVMAHVHTYGELCPKAMPIIHLG
                     ATSCYVGDNTDVILMREGLELVRAKLVKVIARLAKFAEEYKALPTLGFTHFQAAQLVT
                     VGKRATLWMNELLMDLEEVEHRISTLKLLGSKGTTGTQASFLELFEGDHEKVKELERK
                     IAREMGFDAVVPVSGQTYSRKMDYNMVSTLSGIAQSASKFATDMRLLCHLKEVEEPFE
                     KNQIGSSAMPYKRNPMRCERICSLARYVMVDAGNPAVTTATQWFERTLDDSAN"
BASE COUNT         1277 a         1889 c         1846 g         1091 t
ORIGIN      
        1 aggtactgag ccacccgcca ctctgccgga cggaaggcca tgtcgtccac atgggcggac
       61 agcagccgca ccgtccgggc cagatatttc agcatggcca tggccatgcc tggattccgg
      121 gtaaattccg ccgtgacctg ctcccggtcg atggggacga tctcgcaggg ggtgagggct
      181 acggcggagg acacccgggg cagttcgtca aaaaaggatg cctccccgaa gatggagccc
      241 tgctgataca ggttcagggt gcgctccgcc ccgtcctccg actggatgaa gctctttacc
      301 tttccctttt tcaaataata aaagcaggtg gcctctgtcc cctggaggta gatcagatag
      361 ccggaggagc agcggaccga ggggcggtcc tgggcgaagg gacgccagag ctccacaggg
      421 aagtccagcg aaaaggtgtt ggcgtattcc atcgatctct cctcttttcc caagaaatcc
      481 tcttaattgt atcatagtta acattctttt ggcaagagaa ccgtcatact ggacaggaaa
      541 aaatcaccca caaaagtgag gaggcacatt ccatgggaat gacgatgaca caaaaaattc
      601 tggcaaaggc cgccgggctg tcctcagtcc agccgggcca gctgatcgaa gccaggctga
      661 acctggtcct gggcaacgat atcaccaccc ctgtggccat cacggagttt gagaaggctg
      721 gcttcaccca ggtctttgac aaggacagga tcgccattgt gctggaccac tataccccct
      781 gcaaggatat caagtctgcc cagctgtgca agcaggcacg ggagtttgcc cataagcaca
      841 gcatcaccca tttctacgac gtgggccagg tgggcgtaga gcacgccctg atccccgaga
      901 aggggctggc cgccccggga gaggtcatcg tgggggccga ctcccatacc tgcacctacg
      961 gcgcgctggg tgccttctcc accggcatcg gctccaccga catggccgcg ggcatggcca
     1021 ccggcctgct gtggttcaag gtcccctctg ccatcaaggt gaccctgaag gggaagctcc
     1081 agccctgcgt cagcggcaag gatgtgatcc tgcacctgat cggcgagatc ggggtggacg
     1141 gtgccctgta tcagtccctg gagttcgccg gggagggcgt gtcctccctg tccatggacg
     1201 accgcttcac catctccaac atggccatcg aggccggcgg caagaacggc atcttccccg
     1261 tagacgagaa gaccatggag tatatcaacg gccgggtggg ccgcccctgc gaggcctttg
     1321 aggccgatcc cgatgcggtc tacacacggg aggtagtcat cgatctggac aagctggaac
     1381 ccaccgtctc catgccccat ctccccgaga acaccaaggt ggtcagcgag gtggccggcc
     1441 tgcacatcga ccaggtggtg atcggctcct gcaccaatgg gcgcatccag gacctgcggg
     1501 aggccgctgc tattctgaag gggaaaaagg tggcggaaaa tgtgcgctgt atcgtcatcc
     1561 ccgccaccca ggagatcacc ctccaagcca tgaaagaggg gctgctggag atcttcatcg
     1621 cctccggctg cgctgtctcc gcccccacct gcggcccctg cctgggtgga cacatgggcg
     1681 tcatggcgga gggggagcgc tgcgtctcca ccaccaaccg caactttgtg ggccgcatgg
     1741 gtcatgtgca gtcggaagtc atcctggcct ctcccgccgt ggccgccgcc tctgcagtgg
     1801 ccggctgcgt ggccgacccc cgcatctaaa gcgaacgggg aatataaggt cccgaactcc
     1861 tcattcctaa ttcttaattt gtaaagaaag gatcgacgaa ccaatgaagg tttacaaata
     1921 cggtgccaac gtggacactg acgtcatcat ccccgcccgg cacctgaacg acccctctcc
     1981 cgcggccctg gcctcccact gcatggagga catcgataag gacttcgctt ccaccgtcca
     2041 gcccggcgac atcatcgtgg ccggtcctaa ctttggctgc ggctccagcc gtgagcacgc
     2101 ccctctggcc atcaagtcct gcggtgtgaa gtgcgtcatc gccccctcct ttgcccgcat
     2161 cttctaccgc aacgccatca acattggctt ccccattatg gagtgcgccc aagcggcgga
     2221 ggagatccag gccggggacc aggtggaggt ggattttgcc actggtgtca tcaccgatga
     2281 gaccaccggc aagacctacc aggcggcccc cttcccggag tttattgacg gaattatcaa
     2341 aagcggcggc ctgctcaact ccctgaagga ccggggggtg gcgaaatgaa ctacaagatc
     2401 gcgctgatcc ggggcgacgg catcggccca gaggtagtgg gtgaggcggt ccaggtcatg
     2461 gaagccgtgg ggaagaagtt cggccacagc tttacctttg aggacatttt gatgggcggc
     2521 tgcgccactg acgcagtggg ggtgtcctac cccgaaggca ccgcggagaa gtgcagggcc
     2581 tgcgacgcgg tgctgctggg cgccgtaggc ggtcccaagt ggggcagcga caagccagcg
     2641 gaacagcgcc cggagacggc cctgctggcc atccgaaagg acctggggct gtacgccaac
     2701 ctgcgccccg ccgccctgcg tcccgccatg gccggcgcct gccccctgaa gaaggagacc
     2761 gccgagaaag gcatcgacct gatgatggtc cgggagctca ccggcggcat ctacttcggc
     2821 aagcgggacc gctatcagac tgaggacagg ggggaggagg ccaccgacct gatggcctac
     2881 tcagagcagg agatcgagcg catcggccgc cgggcctttg agctggcccg cctgcgccgg
     2941 aacaaggtgt ccagcgtgga caaggccaac gtgctggaga ccagccgcct gtggcggaag
     3001 gtgatgcacc ggctggcgga ggaatactcc gacgtgtcct atgaggacgt gctggtggac
     3061 aactgcgcca tgcagttcgt ccgggacccc ggccagttcg acgtggtggt caccgagaac
     3121 atgttcgggg acatcctctc cgacgaggcc tccatggtca ccggctccat cggcctgctg
     3181 ccctccgcct ctatcgggga cgccgccccc ggcctgtatg agcccatcca cggctccgct
     3241 ccggacatcg ccggccagga caaggccaac cccatcgcca ccatcctgtc tgtggccatg
     3301 atgttccgct actccttcca cctggccgcg gaggcccagg ccatcgaaaa cgccgtggac
     3361 gcggtgctgg ctgagggctg gcgcaccgct gacatcgccg agcccggcgt catccccatc
     3421 gggacccggg agatgggccg gcgcatccgg gaaaagctct gactcagaga aaacacacag
     3481 acccgggacc cccaagccag atccgggagg ggggcccggg tccttttgcc ctctggagga
     3541 acgggagtgg gaacagggca gaggagaatt tggtgaaaaa gcacagaatg gagggagctc
     3601 tacccctgac ttgcaccgtc ccctgccaaa ttgttcagaa tttcaggaaa ataaccggaa
     3661 accacttgca atcctgcaag tcggcgcata taatatgcca tgcgtgattc gccagaagga
     3721 ggacaacatt gatatgaaag agctttccaa gatcgcactg gctgttgagc cgtccagcac
     3781 catggccatc gacactctgt tcaagcagat gaaagccgag gggcaggacg tgatcgggtt
     3841 tggagccgga gaacccgact tcaatacgcc ggactatatc aaagaggcgg gtattgccgc
     3901 catcgagcac aacgagaccc gctacactcc ctccagcggc acggtggagc tgcgcaaggc
     3961 catctgccag cgcctgaagg ccgactgcgg cgtggagtac accccccagc agatctccgt
     4021 gtccaacggc gccaagccct gcgtctatgt ggccctgagg gccctcatca accccgggga
     4081 cgaggtcatc ctgccctctc cctactgggt gagctactct gagctcatca agatgaccgg
     4141 cggcgtgcct gttattgtgg agacccggga ggcggaccat ttcaagctca ccgctcagga
     4201 gctgcgggac gccatcaccc ccaagaccaa ggccatgatc ctgaacaacc catccaaccc
     4261 caccggcatg atgtacagcc gcaaggagct ggaggagatc gccaaggtct gcgtagaaca
     4321 ggatatttac gtgatctgcg acgagatcta ttatcatctg gtgtatgatg gagaggagtt
     4381 cgtcagcctt gcctccatca gcccggagat caaggaacgc accctgctgg tcaatggtgt
     4441 ttccaagtcc tatgccatga ccggctggcg catcggctat gtggccgcta acgaccaggt
     4501 ctccaaggtg atcgccaact acctcagcca ctgcaccggc tcccccagct ccatctccca
     4561 gaaggccgct gtgaccgccc tcactgcctc ccaggagagt gtggaggtca tgcgcaaggc
     4621 tttcgagcgc cgccgtgact acatggtgga gcggatgaac agcatcgagg gcgtcagctg
     4681 catcaagccc cacggcgctt tctatgtgat gatgaacatc gagaagctca tcggccagga
     4741 gctccacggc gtggtcatcc gggacgcgga cgatttcggc aacctcttcc tgaagtacgg
     4801 caaggtggct gtggtgcccg gtactggctt tggtgccccc acttttgttc gctggtccta
     4861 tgccacctcc attgagaaca tcaaggaggg cctgaaccgg ctggagcgtt tcctgaaggg
     4921 cgagaccatc taaacatgac gaaacagagc gggagcacgg acagacccat gtccgcgctc
     4981 ccgctttcct ctcttaagtt ttcgtgaaaa tttgggaaag cactggcaat cccccgggga
     5041 gtacgttata atatttgata caagcgggca ggggagagct gcccagattc taaataaaat
     5101 gaggtttgat gcaaatgccg accaacgcct atgaaagtcc gctgtcctcc cgatatgcca
     5161 gcgacgagat gctgtatctc ttttccgctg acaagaaatt ctccacttgg agacgtctgt
     5221 gggtcgcgct ggcccgggcc gagatggagc tggggctccc ggtgacccag gagcaggtgg
     5281 atgaactgga ggcccatatc actgatatcg actatgagaa ggcggcccag tgggaacgga
     5341 agttgcgcca tgacgtgatg gcccatgtcc acacctacgg tgagctgtgc cccaaagcca
     5401 tgcccatcat ccatctgggg gccaccagct gctatgtggg ggacaatacc gacgtgatcc
     5461 tcatgcggga gggactggag ctggtccggg ccaagctggt aaaggtcatc gcccggctgg
     5521 ccaagttcgc agaggagtat aaggccctgc ccaccctggg ctttactcat tttcaggctg
     5581 cccagctggt gacggtaggc aaacgggcca ccctgtggat gaacgagctg ctcatggatc
     5641 tggaggaggt ggagcaccgc atctccaccc tgaagctgct gggctccaag gggaccaccg
     5701 gtacccaggc atccttcctg gagctgttcg agggggatca cgagaaggtc aaggagctgg
     5761 agcggaagat agcccgtgag atgggctttg acgccgtggt cccggtgagc ggccagacct
     5821 attcccggaa gatggactac aacatggtgt ccaccctgtc cggcatcgct cagtccgcca
     5881 gcaagtttgc caccgacatg cggctgctct gccacctgaa ggaggtggag gagccctttg
     5941 aaaagaacca gatcggctcc tccgccatgc cctacaagcg caaccccatg cgctgcgagc
     6001 gtatctgctc cctggcccgt tacgtcatgg tggacgcggg caaccccgct gtcaccaccg
     6061 cgacccagtg gttcgagcgc accctggatg attccgccaa caa
//
LOCUS       DBMDSJ010000054         6090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3724_length_6090_cov_1.575722, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000054 DBMDSJ010000000
VERSION     DBMDSJ010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3724_length_6090_cov_1.575722"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="ACZPAQ_02220"
     CDS             <1..479
                     /locus_tag="ACZPAQ_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009982955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase
                     domain-containing protein"
                     /protein_id="HIR0902825.1"
                     /translation="PPPFVEVVEVVSAQDMFQAVTSRQEQADFIFKAAAVADYTPADY
                     SDDKVKKREGDLSIPLKRTQDILKHLGEHRRPDQIICGFSMETRDMVENSRAKLEKKK
                     VDMICANNLKVAGAGFGVDTNVLTLITADGAEELPLMSKEEAAGTILDRALAMAGC"
     gene            complement(600..1307)
                     /locus_tag="ACZPAQ_02225"
     CDS             complement(600..1307)
                     /locus_tag="ACZPAQ_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004062234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0902826.1"
                     /translation="MGKLIELEWRKNRIGNWVRWAALLGAALGLLIFAMAFLGIANDP
                     ETGVPDAVPGHGGISSPIELLTSMCFLVFTGVMLSVFIVGAYQNGTMSLVFSYPVRRQ
                     KLLAAQMLAVWIFCFGALAAAKLLLYGCVLAGSRFLPSAFPLDFDMTRGAFYGQLLLR
                     SAVTVTEGFIALFAGLALHSARAAIVTSFLLVLLTQANIGSFTMADSAVFPAVLTGIS
                     LLCALLSLRGAETRDLM"
     gene            complement(1295..2224)
                     /locus_tag="ACZPAQ_02230"
     CDS             complement(1295..2224)
                     /locus_tag="ACZPAQ_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004062233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0902827.1"
                     /translation="MPYILQTDRLTKIIGEKELVRDVDLHVRKGEIYGFLGPNGAGKT
                     TVMKLVTNLWKPTGGRVLLFGEPLTPTSYGVLKRMGSIIEFPTFYPHMTGRENLQIHC
                     DYMGYSRPKGVEEALEQLELDDAADRPVGSYSLGMKQRLGIARALLCRPELLILDEPT
                     NGLDPAGIKQVRDLLKRMREEYEMTVMVSSHILGEMEAMADTVGIIHRGRMREEISLR
                     ELEGRGTSYLHLTVDDPGRASVLLSDVMHLGSFRVMKGGIIRIYDRQASPQEVAGVLA
                     RHHVAVEEIGRRSETLEEHFLKLTSEEGSTWES"
     gene            complement(2291..3241)
                     /locus_tag="ACZPAQ_02235"
     CDS             complement(2291..3241)
                     /locus_tag="ACZPAQ_02235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902828.1"
                     /translation="MDDTVLTALLCAAVAALSGAVLYQRIVLQAQMRQSLEEMTAALG
                     RVLDGESGEQVMVFTQDQTLTGLIAQINRLLEERQRRRAEYRRTEQAVRRMLANISHD
                     IRTPMTVVLGYLEIMRLRKECREELLEKVEARAGEVMELMDRFFTLAKLEAGDMELER
                     SRVDVGEVCRETVLDFYQILTRQEIRVEAEIPEGAVWGCCDREALRRILNNLISNAVR
                     YGGDGGYLGVSLREGEDALFLEVADRGKGVAPGAGEQVFQRLVTLEDSRSSGEGSGLG
                     LSIARELARRMGGDIDLESVPGLRTVFTVRLPKWQDERDS"
     gene            complement(3241..3927)
                     /locus_tag="ACZPAQ_02240"
     CDS             complement(3241..3927)
                     /locus_tag="ACZPAQ_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004062229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0902829.1"
                     /translation="MKLLLVEDDREIQEMLGDYLRTEAYEVVCASNGRDACRLFDEGG
                     IGLVLLDLMIPQVSGMDVMEHIRKKSVVPIIILSARDTEADKTLGLGMGADDYITKPF
                     SVAEVLARVRANIRRVTQYAEPPEKSMVLTAGALRLDGKERTVTKAGERIELTAKEFD
                     ILKLLMANPKRVYTKAQLYTQVWNDAYCRDENAVSVHISRLRGKIEDDPRDPRYVATV
                     WGIGYKLGEK"
     gene            complement(4140..5339)
                     /gene="tuf"
                     /locus_tag="ACZPAQ_02245"
     CDS             complement(4140..5339)
                     /gene="tuf"
                     /locus_tag="ACZPAQ_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR0902830.1"
                     /translation="MAKQKFERTKPHVNIGTIGHVDHGKTTLTAAITKYLALKGQAQY
                     EDYSSIDKAPEERERGITINTAHVEYETDKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVIAATDGPMAQTKEHILLARQVGVPAIVVFLNKCDQVDDEELLELVEMEVRETLSG
                     YEFPGDEIPIIKGSALNALTCEGGVDDPDFACIKELMDAVDDYIPTPDRKADQPFLMP
                     VEDVFTISGRGTVATGRVERGQLKAGEEVEIVGLTDEKKKTVVTSMEMFRKTLDYVEA
                     GDNVGCLLRGVAKTDIERGQVLSKPGSIHPHTKFTGQVYVLSKDEGGRHTPFFNNYRP
                     QFYFRTTDVTGIITLPEGTEMCMPGDNVDMKVELITPIAIEKGLRFAIREGGRTVGSG
                     VVIAIEE"
     gene            complement(5465..>6090)
                     /gene="fusA"
                     /locus_tag="ACZPAQ_02250"
     CDS             complement(5465..>6090)
                     /gene="fusA"
                     /locus_tag="ACZPAQ_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940207.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR0902831.1"
                     /translation="QVEQDTKYARQSGGKGQYGHVKIRVEPNESGKGYEFVNAIVGGA
                     IPKEYIPAVDAGIQGAMQAGVLAGYPVVDVKVTLYDGSFHEVDSSEMAFKIAGSMAFK
                     EACRKANAVLLEPIMKVSVIVPEEYMGDVMGDLNSRRGQILGMEARPGAQQIDALVPL
                     ANMFGYATDLRSSTQGRGQYSMEPHSYVEIPKSIADKIIAERGRSEG"
BASE COUNT         1134 a         2018 c         1724 g         1214 t
ORIGIN      
        1 cccccccccc ctttgtggag gtcgtggagg tagtatccgc ccaagatatg ttccaggctg
       61 tgaccagccg ccaggagcag gcggacttca tctttaaggc cgccgccgtg gccgactaca
      121 cccccgcgga ttacagcgac gacaaggtga aaaagagaga gggcgatctg tctattccgc
      181 tgaaacgcac ccaggatatt ttaaagcacc tgggcgagca ccgccgcccg gaccaaatca
      241 tttgcggctt ctccatggag acccgggaca tggtagagaa cagccgggcc aagctggaga
      301 aaaaaaaggt ggacatgatc tgcgccaaca acctgaaggt ggcaggggcc ggcttcgggg
      361 tggataccaa cgtgctcacc ctcatcaccg cggacggtgc ggaggaactg cccctcatgt
      421 ccaaggagga ggccgccggc accatcctgg accgggccct ggccatggcc ggctgctgag
      481 ccggaccgct gcaagtccca ttttctcccc atacgtgcaa aggaggagcc gcccacagga
      541 atgggcggct cctcctcttc tgttctgatc tgcggccgct cccggtcctt ccaccttcct
      601 tacatcaggt cccgcgtctc cgccccccgc aggctgagca gggcgcacag gagcgagatg
      661 ccggtcagca ccgcgggaaa taccgcgctg tccgccatgg taaagctccc aatgttcgcc
      721 tgggtcagca aaaccagcag gaaggaggtc acaatggcag ctctggccga gtgcagggcc
      781 agccccgcga acaaggcgat aaagccctct gtgaccgtca cggcagagcg caggagcagc
      841 tgcccataga aggctccccg ggtcatgtca aagtccagag ggaaggcgga ggggagaaac
      901 cggctccctg ccagcacaca gccgtagagc agcagcttcg ccgccgccag ggcgccgaag
      961 cagaagatcc acaccgccag catctgggcc gccagcagct tctgccgccg gacggggtag
     1021 gagaacacaa gagacatggt cccgttttga taggctccca cgatgaaaac agagagcatc
     1081 accccggtga acaccaaaaa acacatactg gtcagcagtt cgatggggga cgagatccct
     1141 ccatgccccg gcaccgcgtc aggcacaccg gtctccgggt cgttggcgat ccccaggaaa
     1201 gccatggcaa aaatcagcag gccaagcgcc gccccaagga gagccgccca tctcacccag
     1261 ttcccgatcc ggtttttccg ccattccagt tcgatcagct ttcccatgtg ctgccctcct
     1321 ccgatgtcag cttcaaaaag tgctcctcca gcgtctccga tcttcttccg atctcctcca
     1381 ccgccacatg gtgccgggcc aggacgccgg ccacctcctg gggggacgcc tgccggtcat
     1441 agatccggat gatgccgccc ttcatgaccc ggaagctgcc caagtgcatc acgtcactga
     1501 gcaggacact ggccctcccc gggtcgtcca cggtcagatg gagataagag gtgccccttc
     1561 cctccagctc acggagagag atctcctccc gcatcctccc ccggtggatg atccccacgg
     1621 tgtccgccat ggcctccatc tctccgagga tgtggctgga gaccatcacg gtcatctcat
     1681 attcttctct catccgcttc agcagatccc gcacctgctt gatgcccgcc gggtccaggc
     1741 cgttggtggg ctcgtccagg atcagcagct caggcctgca cagcagggcc cgggcgatcc
     1801 ccagcctctg cttcataccc agggagtagc tccccaccgg ccggtcggca gcgtcgtcca
     1861 gctccagctg ctccagcgcc tcttccacgc ccttgggacg gctgtacccc atatagtcac
     1921 agtggatctg aagattctcc cggcctgtca tgtggggata gaaggtggga aattcgatga
     1981 tgctgcccat ccgcttcagc accccgtagg aggtgggcgt gaggggctct ccgaacagca
     2041 gcacgcggcc cccggtgggc ttccacaggt tggtcaccag cttcatcact gtggtctttc
     2101 ccgccccgtt agggcccagg aatccgtaga tctctccctt ccttacatgg aggtccacgt
     2161 cccggaccag ctctttttct ccaatgatct tggtcaggcg gtccgtttgc agaatatacg
     2221 gcatgtgtct tctgcctcct ttcaccttcc cattgtagtc ccaggacttt tcagaaaatt
     2281 tgcacaattc ttacgaatct ctttcgtctt gccatttggg cagtctgacc gtaaagacag
     2341 tgcggagtcc ggggacactc tccaggtcaa tgtctcctcc cattcgccgg gccagctctc
     2401 tggcgatgga cagccccagt ccgctcccct ctccgctgct tcggctgtct tccaaggtga
     2461 ccaggcgctg gaacacctgc tcccccgctc cgggtgcgac tccctttccg cgatccgcca
     2521 cctccaggaa cagggcgtcc tccccttctc tcagggacac gcccagatat ccgccgtcgc
     2581 cgccgtagcg caccgcgttg gagatcaggt tgttcaggat gcggcgcagc gcctcccgat
     2641 cacagcaccc ccacaccgct ccctccggga tctctgcttc cacgcggatc tcctggcggg
     2701 tcaggatctg gtagaagtcc agcacagtct cccgacagac ctcacctacg tctaccctgg
     2761 accgctccag ctccatatcc cctgcctcca gcttggctaa ggtgaaaaaa cgatccatca
     2821 gctccatgac ctcccccgcc cgggcttcca ccttttccag cagctcctcc cggcactcct
     2881 tccgcagccg catgatctcc aggtacccca gcaccaccgt catgggggtt cggatatcgt
     2941 gggagatgtt ggcgagcata cgccggactg cctgctccgt ccggcggtac tccgcccgcc
     3001 ttctctgccg ttcctccagc agccggttga tctgggcgat caaaccggtc agagtctggt
     3061 cctgggtgaa gaccatgacc tgctccccgc tctccccgtc cagtaccctg cccagggcgg
     3121 cggtcatctc ctccagactt tgtcgcatct gcgcctggag aacgatccgc tgatagagga
     3181 ctgccccgct cagcgccgcc acagcggcac acagcagggc ggtcagcact gtatcgtcca
     3241 tcatttctcc cccaatttgt agccgatgcc ccacactgtc gccacatacc tcgggtcccg
     3301 tgggtcgtcc tcgatcttgc cgcgcagcct gctgatgtgg acgctgaccg cgttctcgtc
     3361 tctgcagtag gcgtcgttcc acacctgggt gtagagctgg gccttggtgt acacacgttt
     3421 aggattcgcc atcagcagct tcaggatgtc aaattccttg gcggtcagct cgatccgctc
     3481 ccccgccttg gtcactgtcc gctccttccc atccaggcgc agcgctccgg ctgtaaggac
     3541 catgctcttc tccggcggct ccgcatactg ggtcacccgc cggatattgg ccctgacccg
     3601 ggccagcacc tccgctacgg agaatggctt tgtgatatag tcatctgccc ccatacccag
     3661 ccccaaggtc ttgtccgcct ccgtgtcccg ggcagacagg atgatgatag gcaccacgct
     3721 cttctttcgg atgtgttcca tcacgtccat cccgctgacc tgagggatca tcagatccag
     3781 cagcaccaga ccgatcccgc cctcgtcgaa cagccggcat gcatcccgcc cgttggacgc
     3841 acagaccact tcataggcct ccgtccgcag atagtccccc agcatctcct gaatctccct
     3901 gtcatcctct accaacagca gcttcattct cgcacctccg ctccactcgg acagtatctc
     3961 tattgtatcc cggtccgggc cagaaatcaa ggaacgaccg gtacgtccgg ccttttctta
     4021 tcggaatggg tacagatcag aagtagggca aagccggatg gatctcaggg ctgccgggct
     4081 ccttcgagaa aaagaaaaat ccctgccaaa ggcagggatt tttccaaaag gagcttgaat
     4141 tactcctcga tggcaatgac aacgccggaa cccacggtgc ggccgccctc acggatggcg
     4201 aaacgcaggc ccttctcgat ggcgatgggg gtgatcagct ccaccttcat gtccacgttg
     4261 tcgccgggca tgcacatctc agtgccctcg ggcagagtga tgatgccggt cacgtcggtg
     4321 gtacggaagt agaactgagg acggtagttg ttgaagaagg gggtatgacg gccgccctcg
     4381 tccttggaca ggacgtacac ctgaccggtg aacttggtgt ggggatggat ggaaccgggc
     4441 ttggacagaa cctggccgcg ctcgatgtcg gtcttggcga cgccgcgcag caggcagccc
     4501 acgttatcgc cggcctcgac gtagtccaga gtcttacgga acatctccat agaggtgacg
     4561 acggtcttct tcttctcgtc ggtcagacca acgatctcga cctcctcgcc ggccttcagc
     4621 tggccgcgct ccacacggcc ggtggcgacg gtgccgcggc cggagatggt gaacacgtcc
     4681 tcgacgggca tcaggaaggg ctggtcggcc ttacggtcgg gggtggggat atagtcgtcg
     4741 acagcgtcca tcagctcctt gatgcaggcg aagtcaggat cgtccacgcc gccctcacag
     4801 gtcagagcgt tcagagcaga gcccttgatg atggggatct cgtcgccggg gaactcatag
     4861 ccggacaggg tctcacggac ctccatctcc accagctcca gcagctcctc gtcgtccacc
     4921 tgatcgcact tgttcaggaa gaccacgatg gcaggcacgc cgacctgacg ggccagcagg
     4981 atgtgctcct tggtctgagc catggggccg tcggtggcgg cgatgaccag gatggcgccg
     5041 tccatctgag cagcgccggt gatcatgttc ttgatatagt cggcgtggcc cgggcagtca
     5101 acgtgagcat agtgacgctt gtcggtctca tactccacgt gggcggtgtt gatggtgatg
     5161 ccgcgctcgc gctcctcggg agccttgtcg atgctggagt agtcctcata ctgagcctgg
     5221 cccttcagag ccagatactt ggtgatggcg gcggtcaggg tggtcttgcc gtggtcaacg
     5281 tggccgatgg tgccgatgtt gacatggggc ttggtgcgct cgaacttctg cttagccatt
     5341 ggaatttcct ccttatatca tttcattcaa atgatgtttt gactgcgata tgatggatat
     5401 cgcaaacatt gtatcgtatt actgggaaaa atgcaactga aatttcccgt aattcctgtg
     5461 aaaatcagcc ctcgctgcgt ccgcgctcgg cgatgatctt gtccgcgatg ctcttgggga
     5521 tctccacata gctgtggggc tccatggagt actggccgcg gccctgggtg gaagaacgca
     5581 ggtcagtggc gtagccgaac atgttggcca gaggcaccag ggcgtcgatc tgctgggcgc
     5641 cgggccgggc ctccatgccc aggatctggc cgcggcggct gttcaggtcg cccatgacgt
     5701 cgcccatata ctcctcgggg acgatgacgg agaccttcat gatgggctcc agcagcacgg
     5761 cgttggcctt gcggcaggcc tccttgaagg ccatggagcc ggcgatctta aaggccatct
     5821 cagaggagtc cacctcgtgg aaggagccgt cgtacagggt gaccttcacg tccaccacgg
     5881 ggtaaccggc cagcacgccg gcctgcatgg ctccctggat accggcatcg accgccggga
     5941 tatactcctt gggaatggcg ccgcccacga tggcgttgac aaactcatag cccttgccgg
     6001 actcgttggg ctccacacgg atcttgacat gaccgtactg gcccttaccg ccggactgac
     6061 gggcgtactt ggtgtcctgc tccacctgct
//
LOCUS       DBMDSJ010000055         6079 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3732_length_6079_cov_2.465206, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000055 DBMDSJ010000000
VERSION     DBMDSJ010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3732_length_6079_cov_2.465206"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /gene="pheS"
                     /locus_tag="ACZPAQ_02255"
     CDS             complement(<1..259)
                     /gene="pheS"
                     /locus_tag="ACZPAQ_02255"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574665.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR0902832.1"
                     /translation="MKEQLARIRSEALAAFEQAQSAAELDSLRVQYLGKKGELTAVLK
                     MMGKLSAEERPAMGQLANEVRSALEAAIEAAAGKLEAQALEQ"
     gene            869..1597
                     /locus_tag="ACZPAQ_02260"
     CDS             869..1597
                     /locus_tag="ACZPAQ_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0902833.1"
                     /translation="MLELQHICYDVEQDGEDKGILRDIDLTIDERFVAITGPNGGGKS
                     TLAKIIAGIYTPTSGRILLDGEDITGLDITQRAHRGVSFAFQQPVRFKGLTVKDLITL
                     ASGKNIGVPEACNYLSEVGLCAKDYIDREVDGSLSGGELKRIEIAMIMARGTKLSVFD
                     EPEAGIDLWSFSNLIQVFEHLHEKINGSILIISHQERILNIADRIIVLADGQVRSDGG
                     REEILPQLLGEAPSVCSALSDKVK"
     gene            1728..2645
                     /locus_tag="ACZPAQ_02265"
     CDS             1728..2645
                     /locus_tag="ACZPAQ_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR0902834.1"
                     /translation="MDAIQKNLLEQVAELHDVPEGAYNIRANGKAAARNTTANIDIVS
                     KEDKDGIDIIIKPGTKNESVHIPVVLSESGLNETVYNDFIIGDDCDVTIVAGCGIHNC
                     GVQNSEHSGIHSFHVGKRARVRYVERHYGEGDGMGENIMNPTTVVEMDEDSYLEMETT
                     QIKGVDSTVRTTKATLGSRATLIIKEKVMTHGKQLAKSDFTVDLNGEDCHTNVISRSV
                     ARDQSHQVFLARINGNARCYGHSECDAIIMDQGVVAAIPEVTANCVDASLVHEAAIGK
                     IAGEQLIKLMTLGLTEKEAEEQIVKGFLK"
     gene            2747..3253
                     /locus_tag="ACZPAQ_02270"
     CDS             2747..3253
                     /locus_tag="ACZPAQ_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902835.1"
                     /translation="MRTIYIDYGEVSQSGPLGIFSCEVQILYAGTVLHTEQAKVRAEV
                     PQYQEMAERAGIYFFFEDEELPEIPFYAVPSLELVARDREGNWYGRAEDLGEGVYCVT
                     PEGAAFRVSERMGRFSGRLLAGEEVRELWEPVPELRVYPSKAEAAREVELVPPEKLLP
                     KGWKERER"
     gene            3250..4935
                     /locus_tag="ACZPAQ_02275"
     CDS             3250..4935
                     /locus_tag="ACZPAQ_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR0902836.1"
                     /translation="MRMLATTEQMRELDRTAIEERGIPSLELMENAAREVAQAVADLV
                     GPVKGPRPDGRIGGAVSVAVLTSRGEPLTPEEQREADEIRAVVESKNSDLTQRIALFC
                     GPGNNGGDGIAAARLLREWGYRVRAFLVGDRTKMTPDARAMEERLAEAGGALEPLDLE
                     DRLLTAWLSTCDCYVDALFGVGLKRPVEGDFLAAVRWMNGRRAPVVSCDLPSGVDGDT
                     GEVLGEAVRAHTTVTFTCAKPGLYLGAGAGCAGEVQEADIGIPWDLVHQMIWRGPEPI
                     ELNPNDMHWVLPRRPADGHKGDFGRVFILGGSEGYTGAPTLAARGALRTGAGLVYVGV
                     PREIYPVIAIKCEEAMAFPLPEEYTAILEKAAACDVAVIGPGLGRHPNTERLARSLLC
                     DLTIPVVLDADGINALAGHIDVLDSRSAPTVLTPHAGEFARLTGGSLPVRDPVAAARG
                     FARAHRCTLVLKGRGTVTAAPEGKVWLCGTGNPGMAKGGSGDVLAGMIAALLGQKHLN
                     GPGVSVPETAALAVWLHGKAGDRCAEQYGEYAMLPSDLIEMLPQVLKESEERN"
     gene            4969..5562
                     /locus_tag="ACZPAQ_02280"
     CDS             4969..5562
                     /locus_tag="ACZPAQ_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902837.1"
                     /translation="MRRRLLCVPMMTLVLLTACGGTAAGDPAEELALAVRGEYLAMER
                     CAAEVEITADYGQRVYEFLLSAAVDGEETVLTLREPELAAGITARISEEGGVLEYDGL
                     ALETGPLDGDGLSPVSALPALLSAARSEYMAECTLERTGDSELLRVLCADPEDPPGTG
                     RETALWFDPETYALVRGEISVDGYRVIQCELTGFTRG"
     gene            5581..>6079
                     /locus_tag="ACZPAQ_02285"
     CDS             5581..>6079
                     /locus_tag="ACZPAQ_02285"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR0902838.1"
                     /translation="MDTTRMRTWAEIDLTALEHNYRTLRAMLPRECRFLGLCKANAYG
                     HGAAVIGHTLERLGADMLAVACVSEAEELRQSGVKLPILCLGETPEELIPLLLELDVT
                     QMVEDLETGRRLSAAAQRAGRTLRVHVKLDTGMSRLGFLWEAGREEETADEIARPASH
                     RKPSRL"
BASE COUNT         1177 a         1735 c         2129 g         1038 t
ORIGIN      
        1 gctgctccag ggcctgggcc tccagcttgc cggcggcggc ctcgatggcc gcctccaggg
       61 cggagcggac ctcgttggcc agctggccca tggcggggcg ctcctcggcg gatagcttgc
      121 ccatcatttt cagaacggcg gtgagctcgc ccttcttgcc cagatactgg acgcgcaggc
      181 tgtccagctc ggcggcggac tgggcctgct caaaggcggc cagcgcctcc gaacggatcc
      241 ttgctaactg ttctttcatc tgtgttcttc ctttcagagg taaattgatc ggacggggac
      301 gggcagcaaa aaagccttcc gccccaaacc tgttgggacg aaaggcaacc cttccgcggt
      361 accacccgta ttcgtgtcag acacgcactt gaatttcctg ataacggcgg aacagccgtc
      421 cccctctcag gggccgctcc cgggcgaacc aaacgacacg cttcggggcg gctttcagcc
      481 ggtgaccgcc cctctctgag aaacgaaaac tcgttatttt cccgttcttc gcatttgaaa
      541 ctgattgtat ctaatactta tagcatactg cggcggaaaa tgcaagtatg aaatgggagc
      601 atccggggga aagaatggaa tatggggaaa atatagatta ggataggaaa tattaagaag
      661 gtccagcggg ggagagcgta tgtgagcagg acaaaacgaa atcctactaa gtaaatagga
      721 aataaggata gacaaaacct tctgtctatg ataaaataag gctgacccta agatagggtg
      781 tcctgtccat ttgtttgtgg gacaaatgga cggggaacgg gaaagacgga accgggatca
      841 aaccggacgg aatacaggag ggaacctcat gctagagtta cagcatatct gctatgatgt
      901 ggagcaggac ggagaggaca aaggcatcct gcgggacatc gatttgacca ttgacgaacg
      961 gtttgtggcc atcaccggcc caaacggcgg cggtaagtcc accctggcca agatcatcgc
     1021 cggcatctac acccccacca gcgggcggat cctgctggac ggggaggaca tcaccggcct
     1081 ggacatcacc cagcgggccc accggggcgt cagctttgcc tttcagcagc cggtgcgctt
     1141 caagggtctg acagtgaagg acctgatcac cctggccagc ggaaaaaaca tcggcgtgcc
     1201 ggaggcgtgc aactacctgt ccgaggtggg gctgtgcgcc aaggactata tcgaccggga
     1261 ggtggacggc tccctgtccg gcggcgaact caagcgtatc gagatcgcca tgatcatggc
     1321 ccgggggacc aagctgtccg tctttgacga gccggaggcg ggtatcgatc tgtggtcctt
     1381 ctccaacctg atccaggtgt tcgagcacct gcacgagaag atcaacggct ctatcctcat
     1441 catctcccat caggagcgga tcctgaacat cgccgaccgc atcatcgttc tggctgacgg
     1501 ccaggtgcgc agcgacgggg gacgggagga gatcctgcct cagctgctgg gcgaggcccc
     1561 cagcgtgtgc agcgcgttga gcgacaaggt aaaataacaa gacgatcagg aattggaaat
     1621 taggaattga cgcgccgatt cggggcggag gtgcctgatt cctccggcag agccggatgc
     1681 agtcattcct aactcctcat tcctaattta cagaaggaga cagaaccatg gacgccatac
     1741 agaaaaattt gctggagcag gtcgccgagc tccatgatgt gcccgaaggg gcctataaca
     1801 tccgggccaa cgggaaggcg gccgcccgga acaccaccgc caacatcgac attgtcagca
     1861 aagaggacaa ggacggcatt gacatcatca tcaagcccgg caccaaaaat gagtcggtcc
     1921 acatccctgt ggtgctcagc gagagcggcc tgaatgagac cgtctacaac gattttatca
     1981 tcggcgacga ctgcgacgtg accatcgtgg ccggctgcgg catccacaac tgcggggtgc
     2041 agaattcgga gcactccggg atccactcct tccatgtggg caagcgcgcc cgggtgcgct
     2101 atgtggagcg ccactacggc gagggcgacg gtatggggga gaacatcatg aatcccacca
     2161 ccgtggtgga gatggatgag gacagctatc tggagatgga gaccacccag atcaagggtg
     2221 tggactccac cgtccgcacc acgaaggcca ccctgggctc ccgggccacc ctcatcatca
     2281 aggagaaggt gatgacccac ggcaagcagc tggccaagtc cgactttact gtggacctga
     2341 acggagagga ctgccacacc aacgtgatct cccgctctgt ggcccgggac cagtcccacc
     2401 aggtgttcct ggcccggatc aacggcaatg cccggtgcta cggccactcc gagtgtgacg
     2461 ccatcatcat ggatcagggc gtggtggccg ccatcccgga ggtcaccgcc aactgcgtgg
     2521 acgccagcct ggtccacgag gctgccatcg gcaagatcgc cggggagcag ctcatcaagc
     2581 tgatgaccct gggcctcacc gagaaggagg ccgaggagca gatcgtcaag ggatttttga
     2641 agtaaatcag acgtccaggg aggggaggcc ggctgcggtc tctcttcctt ttttcatcgg
     2701 gatgtgatac aataccggaa aaatcttttg aaaaggggtg tggaccgtgc ggaccattta
     2761 tattgactac ggcgaggtgt cccaatcggg gccgctggga attttcagct gtgaggtgca
     2821 gatcctttac gctgggacgg tgctccacac ggagcaggcc aaggtccggg cggaggttcc
     2881 acagtatcag gagatggcgg agcgggccgg gatatacttt ttctttgagg atgaggagct
     2941 cccggagatc ccgttttacg cggtccccag cctggagctg gtggcccggg accgggaggg
     3001 aaactggtac ggccgcgcgg aggacctggg ggagggcgtg tactgcgtca ccccggaggg
     3061 agcggctttc cgggtgtccg aacgcatggg ccggttctct ggccgcctgc tggcggggga
     3121 ggaggtccgg gagctgtggg agcctgtccc ggagctcagg gtgtacccct ccaaggcgga
     3181 ggcagcccgg gaggtggagc tggtcccgcc ggagaagctg ctgccaaagg gatggaagga
     3241 gcgggagaga tgaggatgct tgccaccaca gagcagatga gagagctgga ccggaccgcc
     3301 attgaggagc gggggatccc ctctctggag ctgatggaga acgccgcccg ggaggtggcc
     3361 caggcggtgg ccgatctggt ggggccggtg aagggccccc ggccggacgg acggatcggg
     3421 ggcgcggtgt ccgtggcggt gctcacctcg cgtggcgagc cgctgacccc ggaggagcag
     3481 cgggaggcgg acgagatacg ggctgtggtg gagtccaaga acagcgacct cacccagcgg
     3541 atcgctctgt tctgcggccc ggggaacaac ggcggggacg gcatcgccgc cgcccggctg
     3601 ctgcgggagt gggggtatcg ggtgcgggct ttcctggtgg gggaccggac caaaatgacc
     3661 ccagacgccc gggccatgga ggagcggctg gcggaggccg gcggcgctct ggagcctctg
     3721 gatctggagg accgcctgtt gaccgcctgg ctgtctacct gtgactgcta tgtggacgcc
     3781 ctgttcggcg tgggcctgaa gcggccggtg gagggggact ttctggcggc ggtccggtgg
     3841 atgaacggac ggcgggcccc cgtggtgtcc tgcgacctgc cctccggggt ggacggggat
     3901 accggggagg tcctggggga ggccgtccgg gcccacacca ctgtcacctt tacctgtgcc
     3961 aagccggggc tctatctggg ggccggggcg ggctgcgccg gggaggtgca agaggccgac
     4021 atcgggatcc cctgggacct ggtccatcaa atgatatggc ggggtccgga gccgatcgag
     4081 ttaaacccga acgatatgca ctgggtcctc ccccgccgtc ccgctgatgg ccacaagggg
     4141 gattttggaa gggtattcat cctggggggc agcgagggct acaccggcgc cccgactctg
     4201 gccgcccggg gcgcgctgcg gactggcgcg gggctggtct atgtgggggt cccccgggaa
     4261 atctatcctg tcatagccat aaaatgtgaa gaagctatgg cttttccgct gccggaggag
     4321 tatactgcaa tactggaaaa ggcggccgcc tgcgatgtgg cggtgatcgg ccccggactg
     4381 ggccggcatc cgaacacaga gcggctggcg cgttccctgc tgtgcgacct gaccatcccg
     4441 gtggtactgg acgccgatgg cataaacgcg ctggcgggac atatagatgt tctggacagc
     4501 cgctccgctc ccacggtgct caccccccat gcgggggagt ttgcccggct gacgggtggc
     4561 tccctgccgg tgcgggaccc tgtggccgcc gcccgcggct ttgccagggc acaccgctgc
     4621 accctggtcc tcaagggccg ggggacggtg accgcggccc cggagggaaa ggtatggctc
     4681 tgcgggacgg ggaacccggg aatggcaaag ggggggtccg gcgacgtgct ggccgggatg
     4741 atcgccgccc tgctgggaca gaagcacctg aacggtcccg gcgtttctgt accggagact
     4801 gccgccctgg cggtgtggct ccacggaaag gcgggggacc gttgtgcgga gcagtatggg
     4861 gagtacgcca tgctgccctc cgacctgatc gagatgctcc cccaggtgct gaaggagagc
     4921 gaggagcgga actaaacggc cgttttaaga cgctttggag ggaaaactgt gcgcagacgc
     4981 ctgctttgcg taccaatgat gaccctggtg ctgctcaccg cctgcggcgg gacggcggcg
     5041 ggagatccgg cggaggagct ggccctggcg gtccgggggg agtacctggc catggagcgc
     5101 tgtgccgcag aggtggagat caccgccgac tacggccagc gggtctatga atttcttctg
     5161 tccgccgcgg tggatgggga ggagaccgtc ctgaccctgc gggagccgga gctggccgcc
     5221 gggatcaccg cccggatcag cgaagagggc ggagtgctgg agtacgacgg cctggcgctg
     5281 gagaccggac ccctggatgg ggatgggctg tcccccgtgt ccgccctgcc cgcactgttg
     5341 tcggcggccc ggtcggagta catggcggag tgtacgctgg agcggacagg ggactctgag
     5401 ctgctgcggg tgctctgtgc ggatccggag gatccccccg ggactgggcg ggagaccgcc
     5461 ctgtggttcg acccggagac ctatgccctg gtgcgggggg agatcagcgt ggacggatac
     5521 cgggtcatcc agtgcgagtt gaccggcttt accagaggat agagaggaag ctttcagttt
     5581 atggatacca caaggatgag aacatgggcg gagatcgatc tgaccgccct ggagcacaat
     5641 taccggaccc tgcgggccat gctgccccgg gaatgccggt ttctgggcct ctgcaaggcc
     5701 aacgcctatg gacatggggc cgctgtgatc ggtcataccc tggagcgcct gggagcggat
     5761 atgctggcgg tggcctgtgt cagcgaggcg gaggagctgc ggcagagcgg ggtcaagctg
     5821 cccattcttt gcttgggcga gaccccggag gagctgatcc ccctgctgct ggagctggat
     5881 gtgacccaga tggtggagga tctggagacc ggccgaaggc tgtccgccgc cgcccagagg
     5941 gctgggcgga ccctgcgggt ccatgtgaag ctggacaccg gcatgagccg gctgggcttt
     6001 ctgtgggagg cgggccggga ggaggagacg gcggacgaga tcgcccggcc cgcctcccac
     6061 agaaagccca gccggctca
//
LOCUS       DBMDSJ010000056         5992 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3801_length_5992_cov_1.994059, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000056 DBMDSJ010000000
VERSION     DBMDSJ010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3801_length_5992_cov_1.994059"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="ACZPAQ_02290"
     CDS             <1..652
                     /locus_tag="ACZPAQ_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR0902839.1"
                     /translation="GVVLLSTLISHFLHVQVDGFAGLLVAVFILKTGWDSARETLDPL
                     LGRPMDPSLAADIDRLVLSHPHILNIHDLVYHDYGPGRAMMSFHVEVPAGCDLLEIHD
                     VVDHIERELKERHHIETVIHVDPVVNDERTLALRDQVTRLTQELDPVLTIHDFRITAG
                     PLHTNLIFDVVVPYGFRLTDQQVLEQLTRSIRALSDHYYPVIQVDHSYIDQRSVQN"
     gene            810..1514
                     /locus_tag="ACZPAQ_02295"
     CDS             810..1514
                     /locus_tag="ACZPAQ_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0902840.1"
                     /translation="MAKKVLIVEDDSNIAELLNLYLEKEGFEPLVAKDGGKGVVQFRA
                     FQPDLVLLDIMLPVMDGWSVLKKIRESSKVPVIMLTAKGETSDKVSGLEMGADDYIVK
                     PFEMKEVLARIHAVLRRTAGGEEEGGAKKLAFDKLVINLDSYELIVNGQPVDTPPKEL
                     ELLFHLASSPNRVFTRNQLLDEVWGFDYFGDSRTVDVHIKRLREKLEGVSDQWRLKTV
                     WGVGYKFEVSPAGTPG"
     gene            1529..>2580
                     /locus_tag="ACZPAQ_02300"
     CDS             1529..>2580
                     /locus_tag="ACZPAQ_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR0902841.1"
                     /translation="MKSLYKRQLFMMVAIVFLSFFMLSAAFMMLSYRYIIAEKRDAME
                     RNADYIASFTSEYIQQSGSLDVYFRSYVATLASVSDSNVLLSDVDGDIIYASGGSSSD
                     LSNYTDSRIPQRVLQQVLSSGSYNGLTNLDGIYSEKRYLAAYPFGIIFGGATIPQGLV
                     LVSSDTSSVSEMWRATATIFFFTAVTVLLIAVIASSITSSHQVRPLNEMAEAARKFGQ
                     GEFDVRVTGYENRCDEVGTLAEAFNSMAASLSKVETQRSEFIANVSHELKTPMTTISG
                     FAEGILDGTIPPERERDSLEIIVSETRRLSRLVRRMLDLSRLNALTENITAQEQFDLT
                     ETVSQVLVSLEGKITGR"
     assembly_gap    2581..2590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2591..2955
                     /locus_tag="ACZPAQ_02305"
     CDS             <2591..2955
                     /locus_tag="ACZPAQ_02305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902842.1"
                     /translation="MPDEPLKVWGDPDSVTQVCYNLLDNAAKFAAKGTTITVQITKKD
                     GKAYTSIRNLGATIPPDELSLLFDRFHKADYSRSMDREGVGLGLYIVKTILGNLKENI
                     TVTSEDGVTNFTFTLTLA"
     gene            3157..4395
                     /locus_tag="ACZPAQ_02310"
     CDS             3157..4395
                     /locus_tag="ACZPAQ_02310"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR0902843.1"
                     /translation="MDNRSDGLWQGGPWEQPARPFSPPPAVVIPPQKYRPPRPPQHRH
                     SGRIGFLIALALIVSLTALAVLFNGGLAPRSADPVPSGSDPGYSSWEQEEDLSAPPSI
                     PQAETGTGVILSITPPSGEALTYTQVYEKAAPSIAALTAYSAGMVSTGTGIVLTADGY
                     IVTNAHIIAGAEQVNVTLSDDSLWSAQLVGFEPLEDLAVLKIDASGLTPAQFGDDTLL
                     RSGDPVSAIGNPMGYRSTITPGIVSALDQPVSVEGTTMYLLQTSAAINYGSSGGALLN
                     DRGQVVGVTTIKIVADDGSAEGLGFAIPTTRVKQVVDRLIAGAPVTRPSLGIIVRRGQ
                     GENGGLVVEEVDPDSDCHRQGIQPQDIIVAANGQQVQTFADLERIKRTLDVGDSLLLE
                     VLRGGEHLEFTVTLMDQDDF"
     gene            4806..5579
                     /locus_tag="ACZPAQ_02315"
     CDS             4806..5579
                     /locus_tag="ACZPAQ_02315"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR0902844.1"
                     /translation="MAKFIRFLGPDGPTYGRVEDDVVYTLSGDLFGWWEETGEGFLLD
                     GLKLLPPCSPGKMLAVGFNYRDHAEEFHTPIPTDPNIFLKPPSCLAAHGQKVLYPRAL
                     TRQVEYEAELVVVIGRHARHVTPEQAPDYILGYTVGNDVTARDLQSPTGQWALCKGFD
                     TFGPIGPCIVTDVDPRAGLEIESFVNGERKQHSNTRNLIFDPFHLVSYLSQALTLEPG
                     DVIFTGTPSGVSPVHPGDVMEMRIQGIGSLINPVAAEPL"
     gene            complement(5639..>5992)
                     /locus_tag="ACZPAQ_02320"
     CDS             complement(5639..>5992)
                     /locus_tag="ACZPAQ_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_process: GO:0007007 - inner mitochondrial membrane
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR0902845.1"
                     /translation="ELSIFFQKKTGAGFFGGEGFIMQKLSGRGKAFVEIDGSAVEYEL
                     KAGEKMVVDSGNLAMMDATCSIDIQTVKGVKNVLFGGEGLFNTVVTGPGRIVLQTMPI
                     NNFAAAVASVLPSSK"
BASE COUNT         1096 a         2009 c         1702 g         1175 t
ORIGIN      
        1 tggggtcgtt ctcctgtcca ctctgatctc tcacttcctc catgtccagg tggacggctt
       61 cgccggcctg ctggtggcag tgttcatcct gaagactggg tgggattctg ccagggagac
      121 cctggaccct ctgctgggcc gccccatgga cccctcgctg gccgccgata tcgaccggct
      181 ggtcctgtcc cacccccaca tcctcaacat ccacgaccta gtctaccatg actacggccc
      241 cgggcgggcc atgatgtcct tccacgtgga ggtccccgcc ggctgcgacc tgctggagat
      301 ccacgacgta gtggaccaca tcgagcggga actgaaggag cgccaccaca tcgagaccgt
      361 catccacgtg gacccggtgg tcaatgacga gcgcaccctg gccctgcggg accaggtgac
      421 ccggctcacc caggagctgg acccggtcct caccatccac gatttccgca tcacagctgg
      481 tccgctccac accaacctga tctttgacgt ggtggtcccc tatggcttcc gcctcaccga
      541 ccagcaggtg ctggagcagc tcacccggtc gatccgagcc ctgtccgatc actactaccc
      601 ggtgatccag gtggatcact cctatatcga tcagcggagc gtccaaaact gagggatatt
      661 tacaaaaaat tcacccaaga ggggcaaaat cccttataat agaaggagaa cggtgtgatg
      721 cggggccgtg gcacgcgctc ccggtcacag gataaacttt ggggagcgcc ctgtctcttc
      781 cccgaaacat ggaaaaggcg gtgctgatga tggcaaaaaa agttctgatc gtagaggacg
      841 acagcaacat tgcggagctt ctgaacctct acctggaaaa agagggcttt gagcccctgg
      901 tggccaagga cggcggcaag ggggtggtgc agttccgggc tttccagccc gacctggtgc
      961 tgctggacat catgctgccg gtaatggatg gctggtccgt actgaaaaag atccgggaga
     1021 gcagcaaggt gcccgtcatc atgctcaccg ccaaggggga gaccagcgac aaggtctccg
     1081 gcctggaaat gggtgcggac gactatattg tcaagccctt tgagatgaag gaggtcctgg
     1141 cccgcatcca cgctgtgctg cggcgcaccg ccggcggcga ggaggagggg ggcgccaaaa
     1201 aactggcctt tgacaagctg gtcatcaatc tggactccta tgagctgatc gtcaacggcc
     1261 agccggtgga caccccgccc aaggagctgg agctgctgtt ccacctggcc tcctccccca
     1321 accgggtatt cacccgcaac caactgctgg atgaggtgtg gggctttgac tacttcggag
     1381 actcccgcac tgtggacgtg catatcaagc gcctgcggga gaagctggag ggggtcagcg
     1441 accagtggcg tctgaaaacg gtctggggcg tgggctacaa gttcgaggtc tcccccgccg
     1501 ggacgcccgg ctgaggcagg tgtttgcctt gaaaagcctg tataagcgcc agctgttcat
     1561 gatggtggcc atcgtctttc tctccttttt catgctgtcc gccgctttca tgatgctctc
     1621 ctaccgctat atcatcgcag aaaagcggga cgccatggag cgcaacgccg actatatcgc
     1681 ctcctttact tccgaatata tccagcagtc tgggagcctg gatgtctact tccgcagcta
     1741 tgtggctacc ctggcttctg tctccgactc caacgtgctg ctctccgatg tggacgggga
     1801 tatcatctat gcctccggcg gcagctcctc cgacctgtcc aattacaccg acagccgcat
     1861 cccccagcgg gtcctccagc aggtgctgtc cagcggttcc tacaacggcc tgaccaacct
     1921 ggacggcatc tactccgaga agcggtatct ggccgcctac cccttcggca tcatcttcgg
     1981 cggagccacc atccctcagg ggctggtgct ggtctcctcc gacacttcca gcgtctccga
     2041 gatgtggcgt gccaccgcca ccatcttttt ctttaccgct gtgacggtgc tgctcatcgc
     2101 cgtgatcgcc agttccatca cctcctccca tcaggtccgt ccgctgaacg agatggcaga
     2161 ggccgcccga aaattcgggc agggggagtt cgacgtgcgg gtcacaggct atgagaaccg
     2221 gtgcgacgag gtgggcaccc tggcggaggc tttcaactcc atggccgcct ccctgtccaa
     2281 ggtggagacc cagcgcagcg agttcatcgc caatgtctcc cacgagctca agacccccat
     2341 gaccaccatc tccggctttg cggagggcat cctggacggc accatccccc cggagcggga
     2401 acgggactcc ctggagatca tcgtctctga gacccgccgc ctgtcccggc tggtgcggcg
     2461 gatgctggac ctgtcccgcc tcaatgcttt gacggagaac atcaccgctc aggagcagtt
     2521 cgacctcacg gagaccgtct cccaggtatt ggtcagcctg gagggcaaga tcaccggacg
     2581 nnnnnnnnnn aaatgccgga cgagcccctg aaggtgtggg gcgacccgga ttcggtgacc
     2641 caggtgtgct acaacctgct ggacaacgcc gccaaatttg ccgccaaggg gaccaccatc
     2701 acggtccaga tcaccaaaaa ggacggcaag gcgtacacct ccatccgcaa tctgggggcc
     2761 accatccctc cagacgagct ctctctcctc tttgaccgct tccacaaggc ggactactcc
     2821 agaagcatgg accgggaggg cgtgggcctg ggtctgtata tcgtcaagac cattttgggc
     2881 aatctaaaag aaaacatcac cgtgaccagt gaggacggtg tcaccaactt taccttcacc
     2941 ctcaccctcg cctgagttct tccaggctcc tctgtgacgt tttctttggg cctgcggcgc
     3001 gggccccttt cgtccggccg cgtatgtttg tcccccctgc ggcatattct ttgactggaa
     3061 gggcgtgcgg gcggcccttc gcccccatgt ccccctgctc cgagcaggaa tcttcacact
     3121 gaactgagcg ccccagggcg cggggaggtt tcttctatgg acaatcgaag cgatgggctc
     3181 tggcagggcg gcccctggga gcagcctgcc cgccccttct ccccgccgcc cgccgtggta
     3241 atcccgcccc agaaataccg tcctcccagg ccgccccaac accgccacag cggtcggatc
     3301 ggctttctca tcgcgctggc tttgatcgtc tccctcaccg cccttgcagt cttgttcaat
     3361 ggcggcctcg ccccccgttc cgcggacccc gtcccctctg ggtccgaccc gggatacagc
     3421 agctgggagc aggaggagga cctctccgcg cccccttcca ttccgcaggc ggagaccggc
     3481 accggcgtca tcctctccat tactcccccc tccggtgagg cgctgaccta cacccaggtc
     3541 tatgaaaagg ccgccccctc cattgcggcc ttgaccgcat acagcgccgg aatggtctct
     3601 accggtactg ggatcgtact cactgccgac ggatatatcg ttaccaacgc ccatatcatc
     3661 gccggcgccg aacaggtcaa cgtcaccctc tccgacgact cgctctggtc cgcccagttg
     3721 gtgggcttcg agcctttgga ggatctggcg gtgctgaaga tcgacgcttc cggcctcacc
     3781 cccgcccaat ttggcgatga caccctgctc cgcagcggag accccgtctc cgccatcggc
     3841 aaccccatgg gctaccgctc caccatcacg ccgggcatcg tgtctgccct ggatcagccg
     3901 gtatcagtgg aagggaccac catgtacctc ctccagacca gcgcagccat caactacggc
     3961 agctccgggg gcgccctgct caatgaccgg ggccaggtgg tgggggtgac caccatcaag
     4021 atcgtggcag acgacggctc tgccgagggg ctgggcttcg ccatccccac cacccgggtc
     4081 aagcaggtgg tggaccgcct catcgctgga gcgccggtca cccgcccctc cctggggatc
     4141 atcgtacgcc ggggccaggg ggagaacggc ggtctggtgg tggaggaggt agatccggac
     4201 agcgactgcc accggcaggg cattcagccc caggatatca tcgtggcggc caacgggcag
     4261 caggtccaga ccttcgccga cctggagcgg atcaagcgca ccctggacgt gggcgactcc
     4321 ctgctcctgg aggtcctgcg gggcggggag cacctggagt tcaccgtcac tctgatggat
     4381 caggacgact tctgactccg tccccgtcca agcgcgtctc ctggtccggg agatacgctt
     4441 attctgtggg gaaatcctcc atggacgttt tccacagcat gcatcccgct acttttccgc
     4501 ccgctctggg gcggaaaagt cctcgctttg ctcgccgcag cccttgacgt acaaaggctg
     4561 cgggggatgt gatctcattc gaggcgggct gccgccccct cgaactcccc tgtctgatcc
     4621 tgaaacatct gtccctgaaa aaagcttacg aaaagccgag tgcgtctccc gatccgggag
     4681 acgcactttt gtccccttgt ccgccgtgcc cggagcatct tcctttccac ttgccttttt
     4741 tctccgtccg gagtacacta gaagcaggaa gcatccttca ttctgagtgc gggaggtaat
     4801 gcaagatggc aaaattcatt cgttttctgg ggccggacgg ccccacctat ggccgcgtgg
     4861 aggacgacgt ggtctacacc ctgtccggtg acctgtttgg ctggtgggag gagaccggag
     4921 agggttttct cctggacggt ctgaagctcc tgcccccctg ctctcccggc aagatgctgg
     4981 cggttggctt caactaccgg gatcacgccg aggagttcca cacccccatt cccaccgacc
     5041 caaacatctt ccttaagccg cccagctgcc tggccgccca tgggcagaag gtcctctatc
     5101 cccgtgccct gacccggcag gtagagtatg aggcggagct ggtagtggtc attggccggc
     5161 atgcccgcca tgtcaccccg gagcaggccc cggactatat tctgggctac accgtgggca
     5221 acgacgtcac cgcccgggat ctccagtccc ccaccgggca gtgggcgctg tgcaagggct
     5281 ttgatacctt cggccccatc ggtccctgta tcgtcaccga tgtggacccc cgggccgggc
     5341 tggagatcga gagctttgtc aacggggagc ggaagcagca ctccaacacc cgcaatctca
     5401 tctttgaccc cttccacctg gtgagctatc tctctcaggc cctcaccctg gaacctggcg
     5461 acgtgatctt caccggcacc ccctccggtg tcagtccggt ccatcccggc gacgtgatgg
     5521 agatgcgcat ccagggcatc ggctctctca tcaatccggt agccgccgaa cccctgtgag
     5581 cagaaaaatc gctctcccag acctttgagt ccggaagagc gatttttctg ctccttgttt
     5641 acttgctgct gggcaggaca gacgccaccg ccgccgcaaa attattgatg ggcatagtct
     5701 ggagcacgat gcgccccggg ccggtgacca cggtgttgaa cagcccctca ccgccaaaga
     5761 ggacgttttt gacgcccttc accgtctgga tgtcaatgga gcaggttgcg tccatcatgg
     5821 ccaggtttcc gctgtctacc accatcttct cccccgcctt cagctcgtac tccaccgcag
     5881 agccgtcgat ctccacgaaa gccttgccac ggccggacag cttctgcatg atgaagccct
     5941 cgccgccgaa aaatccggcg cccgttttct tctggaagaa gatggacagc tc
//
LOCUS       DBMDSJ010000057         5920 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3847_length_5920_cov_1.778656, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000057 DBMDSJ010000000
VERSION     DBMDSJ010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3847_length_5920_cov_1.778656"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="ACZPAQ_02325"
     CDS             complement(<1..691)
                     /locus_tag="ACZPAQ_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR0902846.1"
                     /translation="MARGERNAGWSGFFLTFTVFAAILLLLAREGQGEAVSAGARQVL
                     VIYEGLTCWAVPALFMAWGMAALEEGKPSLTGALAGLVLPAFCLLLFWGAAYALAAHL
                     LGGGAVSWSGILGALRSAALGDVYFHLRVLYPLLGLYLVHPVLRRFTAAASRGEVLYF
                     LLLCFLFASLLPAWTAFHPEGVVGALLERLQVHLVLGYAGYYVAGWYLRHYTIGRVPE
                     FLLYLLGILGLV"
     gene            complement(692..1060)
                     /locus_tag="ACZPAQ_02330"
     CDS             complement(692..1060)
                     /locus_tag="ACZPAQ_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR0902847.1"
                     /translation="MIKFNHFNFNVLDLDKSLAFYKEALGLEPVREKEASDGSFKLVY
                     LGDGLTEFQLELTWLKDRDKPYDLGECEFHLAFVTDQYDDFYAKHKEMGCVCFENPGM
                     GIYFIADPDGYWIEIVPEKK"
     gene            1247..2146
                     /locus_tag="ACZPAQ_02335"
     CDS             1247..2146
                     /locus_tag="ACZPAQ_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015436.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR0902848.1"
                     /translation="MSSDHIWPILFFVSLAGNLILLFYRPLLRLLRRGEEVSEDEIMA
                     MVEEGEESGAIERNEKELIENVFEFNNTTAEDVMIHRTDMVTLSLDDDEETILSAIRE
                     SGLSRFPVYQGNVDDIVGILSTRQYLLNFHVERPQELRALLRPAYFVPETVRADVLFR
                     DMQSKKIHMAVVVDEYGGTSGLVTLEDLLEELVGNIYDEFDPQAEQEIIQLEENLWRV
                     SGSADLEELAEAMDFQLPEDEDRDYDTLGGLVFAQLAVIPEDGSRPVVEALGLRIRVE
                     ELCDRRVEWALVEKQAPAAAACE"
     gene            2238..3011
                     /locus_tag="ACZPAQ_02340"
     CDS             2238..3011
                     /locus_tag="ACZPAQ_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR0902849.1"
                     /translation="MTKAIFFDVDGTLVSFKTHQISPAVRQALQALRERGIKLFLSTG
                     RHLAMLDPVRQVFDFDGYVTLSGQYCTAQGQVLRSTPMPPEAVEELVAAARTDAFSCI
                     FLEGEEIYINCINEMTRQFIRDLSIPMPLQRPADYARGREVYQAVTFLTREREHLLLD
                     RAPHLKTTRWHPNFLDVIPPSGGKDRGMDALLDYFRIPVEDAMAFGDGENDLSMLLHA
                     GIGVAMGSAHEEMKRQVDWVTGTADEDGVVQALAHFGLI"
     gene            3099..4697
                     /locus_tag="ACZPAQ_02345"
     CDS             3099..4697
                     /locus_tag="ACZPAQ_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR0902850.1"
                     /translation="MKLSFFGAAHAVTGSCHCLEANGRKILIDCGLQQGRDEHDDNAL
                     DFAPGQIDYCLVTHAHIDHSGRLPLLVKEGFQGQIITTRLTAQLLSIMLRDSAHIQES
                     DAQWQNQKGKRAGRDAVEPLYTVADAEAALQQLYPVEYGQVLDLCEGVRVRFTDAGHL
                     LGSSEVELWLTEGDVERKIVFSGDLGNIDQPIIRDPSFVEEADYVVMESTYGDRNHEP
                     PESYTESLAQLIDEVFAKGGNIVIPSFAVGRTQELLYFLREIKDRGLVKSAPNFTVCV
                     DSPLAAEATRVYSGDLHGYLDEEAIAVLQGGDDLFTFPGLTLTQSTEESKALNMDPSP
                     KIIISASGMCDAGRIRHHLKHNLWRPECAVVFVGYQAEGSLGRRLLEGAKTVKLFGEE
                     IAVRARIVNFKGLSSHADRDHLLEWVGRIAPAPRQVFVVHGDAPVTELFAEDLNQRGI
                     PAHAPLYQEVYDLAADRMLAKGVVLESKRTTGGASAPSAAYVRLVDVTKQLQDMVGRS
                     RGRANKDLGRLADQLKAIMEKWDA"
     gene            4728..5597
                     /locus_tag="ACZPAQ_02350"
     CDS             4728..5597
                     /locus_tag="ACZPAQ_02350"
                     /inference="COORDINATES: protein motif:HMM:NF017927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR0902851.1"
                     /translation="MKIDRKGLKAQAKQAIRAAAPVFWLVTLVYLLLTEGLSTVVGWL
                     APTFQPADLLWGGTVGWLSLFISILLTLYLWVMDFGYRLWALRVTRSQQAGYGTLLEG
                     FGIAGRVIAMNLLILLFTFLWSLLLIFVTTILAFFVLDSLFLQLLVMAGIYAAVFAIM
                     LRYAMAPYLLADYPDDGAGAAVRRSVEMMRGRKWELFKLYVSFLGWELLGVLLTLLAY
                     LPFLPGILAQVNSVAQFYSVLSSLIPATGLALLINLPLTLWLTPYRTAAEALFYRSIL
                     EGRPAAPETEAQS"
BASE COUNT         1079 a         1869 c         1891 g         1081 t
ORIGIN      
        1 ggaccagccc caggatgccc agcaggtaga gcaggaactc cggcacccgc ccgatggtat
       61 agtggcgcag ataccacccc gccacataat agcccgcata ccccagcacc agatggacct
      121 ggagcctctc cagcagggca cccaccaccc cctccgggtg gaaggccgtc caggccggca
      181 gcaggctggc aaagaggaag cacaggagga gaaaatacag cacctccccc cggctggccg
      241 cggcggtgaa ccggcgcagc accgggtgta ccagatacag gccgagcagc gggtagagga
      301 cccgcaggtg gaaatagaca tcccccaggg cggcggaccg cagggcaccc aggatgccgg
      361 accagctcac cgccccgccg cccagcaggt gggccgccag ggcgtacgcc gcaccccaga
      421 agagcagcag gcagaaggcc ggcaggacca gccccgccag cgccccggtg agggatggct
      481 ttccctcctc cagggcagcc atcccccagg ccatgaacag ggccggcacc gcccagcagg
      541 tgagtccctc gtagatgacg agcacctgcc gcgccccggc ggagaccgcc tccccctggc
      601 cctctcgggc caggagcagc aggatggcgg caaagacggt gaaggtcagg aaaaagccgg
      661 accagccggc gtttcgctct ccccgggcca tttatttctt ctccggcacg atctcgatcc
      721 aatagccgtc cgggtcggca atgaagtaga tgcccatgcc cgggttttca aagcagacgc
      781 agcccatctc cttgtgctta gcataaaagt cgtcgtactg gtcggtgacg aaagccaggt
      841 ggaactcgca ctctcccagg tcgtagggct tgtcccggtc cttcagccag gtcagctcca
      901 gctggaactc cgtcaggccg tcccccaggt agaccaattt gaacgagccg tcggaggcct
      961 ccttctcccg caccggctcc aggcccagtg cctccttgta gaaggccagg ctcttgtcca
     1021 gatccaggac gttgaaattg aagtggttga atttgatcat gttcattcgc tcctttccgg
     1081 tcgggacgac cggtgtcttt ttatcattat atcgggcaca agccgatttt tcaacggaaa
     1141 agtgtccaaa gacaggcaag acctctgttt tgtccccttg cggggcgggg caaatcgtgg
     1201 tatgatagtt tatattttat tccccgggta ttaaggagga atttccgtgt catcggacca
     1261 tatatggccc atcctgtttt ttgtcagcct ggccggcaac ctgatcctgc tgttctaccg
     1321 ccctctgctg aggctgcttc gcagggggga ggaggtctcg gaggacgaga tcatggccat
     1381 ggtggaggag ggcgaggagt ccggcgccat cgagcgcaac gagaaggaac tgatcgagaa
     1441 cgtcttcgag ttcaacaaca ccacagcgga ggatgtgatg atccaccgca ccgacatggt
     1501 gaccctctcc ctggatgacg atgaggagac cattctctcc gccattcggg agtccggtct
     1561 gtcccgcttc ccggtctatc agggaaacgt ggacgacatc gtgggcatcc tctccacccg
     1621 ccagtatctg ctcaacttcc atgtggaacg tccccaggag ctgcgggcgc tgctgcgccc
     1681 ggcctacttc gtgccggaga cggtgcgggc tgacgtgctc ttccgtgaca tgcagagcaa
     1741 aaagatccac atggccgtgg tggtggatga gtatggcggg accagcggac tggtgaccct
     1801 ggaggacctg ctggaggagc tggtgggcaa tatctatgac gagtttgacc cccaggccga
     1861 gcaggagatc atccagctgg aggagaacct gtggcgggtc tccggctccg ctgacctgga
     1921 ggagctggcc gaggccatgg acttccagct gcccgaggac gaggaccggg actatgatac
     1981 cctgggcggg ctggtctttg cccagctggc ggtcatcccg gaggatggga gccgccccgt
     2041 ggtggaggcc ctgggcctgc gcatccgggt ggaggagctg tgcgaccgcc gggtggagtg
     2101 ggccctggtg gaaaagcagg cgcccgctgc tgccgcctgc gaatagagac gcgcttcagg
     2161 actgccgcgg ccttgcctgc ggcggtcctg ttttttccgc cccgggcggg cagatcagag
     2221 agaaaggaac ttcacccatg acaaaggcaa tctttttcga tgtagacggc acgctggtca
     2281 gcttcaagac ccatcagatc tccccagccg tccgccaggc cctccaggcc ctgcgggagc
     2341 gggggatcaa gctgttcctg tccactgggc ggcacctcgc catgctcgat ccggtgcgac
     2401 aggtctttga cttcgacggc tatgtgaccc tcagcggcca gtactgcacg gcccagggac
     2461 aggtgctgcg gagcactccc atgccccctg aggcggtgga ggagctggtg gccgccgccc
     2521 ggaccgacgc cttctcctgc atcttcctgg agggggagga gatctatatc aactgcatca
     2581 acgagatgac ccggcagttc atccgggatc tgtccatccc tatgccgctc cagcgccctg
     2641 cggactacgc ccggggccgg gaggtctatc aggcggtgac cttcctgacc cgggagcggg
     2701 agcatctgct gctggaccgg gcccctcacc tgaagaccac ccggtggcac cccaatttcc
     2761 tggacgtgat cccgcccagc gggggcaagg accgggggat ggacgcgctt ctggactact
     2821 tccgcatccc ggtggaggac gctatggctt tcggagacgg ggaaaacgac ctgtccatgc
     2881 tgctccacgc gggcatcggg gtggccatgg ggagcgccca cgaggagatg aagcggcagg
     2941 tggactgggt caccggaaca gcagacgagg acggcgtggt ccaggcgctg gcccatttcg
     3001 gcctgatttg agggcggcga gagattgtac tggaactttc gacaaaaaag gtgtacaata
     3061 aaactgaaaa atcggcatat aaaaaggagg acactggtat gaagctgagt ttcttcggcg
     3121 cggcccacgc tgtcacgggg agctgccact gtctggaagc caacggcagg aagatcctca
     3181 tcgactgcgg cttgcagcag ggccgggacg agcatgacga caacgccctg gacttcgccc
     3241 ccggccagat cgattactgc ctggtcaccc acgcccacat cgaccactcc ggccggctgc
     3301 cactgctggt caaggagggg ttccaggggc agatcatcac cacccgtctc accgcccagc
     3361 tcctgtccat catgctgcgg gactcggccc atatccagga gagcgacgcc cagtggcaga
     3421 accagaaggg caagcgggcc ggccgtgacg cagtggagcc tctctacact gtggctgacg
     3481 cggaggccgc cctccagcag ctctatcccg tggagtacgg ccaggtgctg gatctgtgcg
     3541 agggggtccg ggtccgcttc accgatgcgg gtcacctgct gggctcctct gaggtggagc
     3601 tgtggctcac cgagggggat gtggagcgga agatcgtctt ctccggcgac ctgggcaaca
     3661 tcgaccagcc cattatccgc gacccttcct ttgtggagga ggcggactat gtggttatgg
     3721 agtccaccta tggcgaccgg aaccatgagc cgccggagag ctacaccgag tctctggccc
     3781 agctcatcga cgaggtgttc gccaagggtg ggaacatcgt gatcccctcc ttcgcggtgg
     3841 gccgcaccca ggagctgctc tacttcctcc gggagatcaa ggaccgtggg ctggtgaaga
     3901 gcgcccccaa cttcaccgtg tgcgtggaca gcccgctggc tgcggaggcc acccgggtgt
     3961 actccggcga cctccacggc tatctggacg aggaggccat tgcggtgctc caggggggag
     4021 atgacctgtt caccttccct ggcctgaccc tgacccagtc cacggaggag tccaaggcac
     4081 tgaatatgga cccgtccccc aagatcatca tctccgcctc cggcatgtgc gacgccggcc
     4141 gcatccgtca ccacctgaag cataacctgt ggcggccgga gtgcgcggtg gtcttcgtgg
     4201 gctatcaggc ggagggctcc ctgggccgcc gcctgctgga gggcgccaag acggtgaagc
     4261 tgttcggcga ggagatcgcc gtccgggccc gtatcgtcaa cttcaagggg ctcagctccc
     4321 acgcggaccg ggatcacctg ctggagtggg tgggccgcat cgcgccggcg ccccggcagg
     4381 tctttgtggt ccacggagac gcgccggtca ccgagctgtt tgctgaggac ctgaaccagc
     4441 ggggcatccc cgcccacgca cctctgtacc aggaggtcta cgacctggct gcggaccgga
     4501 tgctggccaa gggcgtggtg ctggagagca agcggaccac tggcggcgcc tccgcccctt
     4561 cggcggccta tgtccgcctg gtggacgtga ccaaacagct ccaggatatg gtgggccgca
     4621 gccgcggccg ggccaacaag gacctgggac ggctggccga ccagctgaag gccatcatgg
     4681 agaaatggga tgcttgatct gccaccgggc aggagagagg agcgtatatg aagatcgacc
     4741 gaaaaggcct gaaggcgcag gccaagcagg ccatccgtgc ggcggccccc gtgttctggc
     4801 tggtcaccct ggtgtatctg ctgctgaccg aaggattgag cactgtggta ggctggctcg
     4861 cccccacctt ccagcctgcg gacctgcttt ggggcggaac tgtgggatgg ctcagcttgt
     4921 ttatctccat cctgctgacc ctctatctgt gggtgatgga ctttggctat cgcctgtggg
     4981 ccctccgcgt cacccgcagc cagcaggcgg ggtatgggac gctgctggag ggtttcggca
     5041 tcgcagggcg ggtcattgcc atgaacctgc tgatcctgct gttcaccttc ctgtggtccc
     5101 tgcttctgat ctttgtgacc accatcctgg ctttttttgt ccttgattcc ctgtttctgc
     5161 aactgctggt gatggcagga atctatgcgg cagtctttgc catcatgctc cgctatgcca
     5221 tggcccccta cctgctggcc gactacccgg acgacggcgc aggggctgcg gtccgccgca
     5281 gcgtggagat gatgcggggg cgcaagtggg agctgttcaa gctgtatgtt tccttcctgg
     5341 gctgggaact gctgggcgtc ctgctcaccc tgctggccta tctgcccttc ctccccggta
     5401 tcctggccca ggtcaactcg gtcgcgcagt tctactccgt cctgtccagt ctcatcccgg
     5461 ccactggtct ggccctcctg atcaatctgc ccctcaccct gtggctgacc ccctaccgca
     5521 cggcggcgga ggccctgttt taccgctcca tcctggaggg acggccggcc gccccggaga
     5581 cggaggcgca gagctgatgt ccgtcggaat gtgggcacag cgcagccgat aaatggatat
     5641 gagagcggcg cctggccggg cgccgctctc tttcgacaaa accgcaggca cggttcgtca
     5701 ttttttgagc aaaatgtgaa ttttcccgga tctactggac attttcccgg gacacgagta
     5761 tgatattgtc actatgtacg cgccctcggg cgcccatcca tcggcacaga gggccgcgcc
     5821 cctgtgcagg gaggtaatcc atggccagtt ccatgttcgg cgggatacac cccgcttccc
     5881 gaaaagaaaa tacccggcgc aagccgctga tgctgctgga
//
LOCUS       DBMDSJ010000058         5901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3859_length_5901_cov_1.676897, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000058 DBMDSJ010000000
VERSION     DBMDSJ010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3859_length_5901_cov_1.676897"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(7..711)
                     /locus_tag="ACZPAQ_02355"
     CDS             complement(7..711)
                     /locus_tag="ACZPAQ_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0902852.1"
                     /translation="MGKSILIVEDEQNIVDILSFNLSREGYDTLEAYDGPTGLQLALE
                     QDPDLILLDLMLPGMDGFEVCRQVRESGSSTPIIMLTAREEETDKVLGLELGADDYIT
                     KPFSMRELLARVKANIRRMSMVPAGKGAAASANRMELGRVTVDTDQMAVLKDGQPLQL
                     SQREYDLIRFLAAQPGKVFSREALMEHVWDYEGYVGDVRAVDVAVRRLREKIEDDPAS
                     PKFVVTKLGMGYYFGG"
     gene            complement(736..1272)
                     /locus_tag="ACZPAQ_02360"
     CDS             complement(736..1272)
                     /locus_tag="ACZPAQ_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR0902853.1"
                     /translation="MDLTERTIESKTMFQGRIITVLLDTAGLPDGGTAQREVVLHPGG
                     VAVLALDEADNVALVRQYRYPLHGLLLELPAGKLDPGEDHRPAALRELSEETGLEAGE
                     LTYLGHIYASPGFCDEALHMYLARDLTRARQHLDEDEFLDVVTMPFDTLVEQVMSGEI
                     TDAKTVATVLKVKTLLGR"
     gene            complement(1431..1868)
                     /locus_tag="ACZPAQ_02365"
     CDS             complement(1431..1868)
                     /locus_tag="ACZPAQ_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902854.1"
                     /translation="MEQEKRQKEITRHGLIRVGLWLGGGWLLLVLCAWGILPDREVPG
                     ILWVGLGLLTAELAILWGKTWWESRGRGQAIPAGAHWWQAEKEELAAAAVMLAVWWGF
                     LALCAAGVLPHMEPDRYSLLLAGAVTILFGAELLLWQRGRRDR"
     gene            complement(1889..2674)
                     /gene="udp"
                     /locus_tag="ACZPAQ_02370"
     CDS             complement(1889..2674)
                     /gene="udp"
                     /locus_tag="ACZPAQ_02370"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HIR0902855.1"
                     /translation="MQERALQFHIQCGPGDVGGYCVLPGDPGRCRSIAQRLEGAVHVR
                     TNREFETWTGSLLGEKVSVVSTGIGGPSAAIAMEELANLGTHTFVRVGTCGGIRLDVR
                     SGDVVVATGAVRMEGTSREYAPIEYPAVPDFQVAGALVQACQALGKPWHAGVVQCKDS
                     FYGQHDPARMPVSGELEAKWEAWKRLGVLASEMESAALFTVAAARGVRCGSVFHVIWN
                     QEREQAGLDQQESHDTALAIDVGVEALKLLIRQDAQSRAGEGR"
     gene            complement(2976..4349)
                     /locus_tag="ACZPAQ_02375"
     CDS             complement(2976..4349)
                     /locus_tag="ACZPAQ_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR0902856.1"
                     /translation="MIEIITKINSAVNNFVWGPVMLLLLVGTGVYLTARTGCIQAAKF
                     GYIMRNTVGSLFRRREKRDKDAKNLTPFQAVTTALAGTVGTGNIAGVTGAIFAGGPGA
                     VFWMWFAAFFGMFTKFAEIVLALKYRQVDENGVHYGGPMYYIEKGLGMKWLAVLFSLV
                     AGFACFGIGNISQSSEIAGAMEGLFEMSGLTTGVLLAVVVGIVVLGGVQRIGRVTSYM
                     VPFMAIFYIGAGLAVILLRIDQVPAALASIFTSAFSFEAVGGGVFGYAIMVAMRQGFA
                     RGVFSNEAGLGSAPMAHAASETQEPVEQGMWGVFEVFIDTIVICTLTSLAVILSGVLD
                     VGLDSYATNGAAAVAAFNAILPGTIGGTIIQISLLFFALSTILGWSYYGERCWAYLSN
                     NNKAVVWVFKVVFVLVCIVGATGNGTLMWDISDTLNGMMAIPNLIGLLLLSNVIIRST
                     NDYFKKH"
     gene            complement(4579..4923)
                     /locus_tag="ACZPAQ_02380"
     CDS             complement(4579..4923)
                     /locus_tag="ACZPAQ_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902857.1"
                     /translation="MNDLREVDLERGMPRAEQAIRQMLFELRRSPGLGCSGVKLIHGY
                     GSSGAGGRIRVEARRRLDRMKAGGEIWGYIPGEFFSIFDSDTLAAFQRCPTLRRDRDL
                     ERHNNGVTFVLL"
     gene            complement(4916..5515)
                     /locus_tag="ACZPAQ_02385"
     CDS             complement(4916..5515)
                     /locus_tag="ACZPAQ_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015674.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR0902858.1"
                     /translation="MKTILQEQRRSSVIVALITMVLGAVLVVWPDRSVRLMCTLLGAA
                     LLICGLVYILGWLVRRREGGISAFTLIPGVVLSGLGIWLMTSSDSVIALVQYVFGAVV
                     IFHGVLDVQSSVSLMRQGAARWWLDLALSALTLALGALILINPFGTFAALVTLIGLVL
                     IYDGLSDLWIIHRLSRMVRDLERDLNGDIIETRGRDLDE"
     gene            complement(5558..>5901)
                     /locus_tag="ACZPAQ_02390"
     CDS             complement(5558..>5901)
                     /locus_tag="ACZPAQ_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR0902859.1"
                     /translation="LERDIPFTFHQDTPVLPPDMLETVWCGVNRLTRSGAVLGPEQKI
                     PVVEALAAVTIRAAWQYFEEGEKGSLREGKRADLVLLERDPLTADPMSLRDIPVLATW
                     KDGRLIYRREG"
BASE COUNT         1067 a         2136 c         1677 g         1021 t
ORIGIN      
        1 tccggctcag ccgccgaagt agtaccccat ccccagcttt gtcaccacga atttaggact
       61 ggcggggtcg tcctcgatct tctctctcag gcggcggacg gccacgtcca ccgcccgcac
      121 atcgcccaca tagccttcat agtcccacac atgctccatc agggcctccc gtgaaaacac
      181 cttgcccggc tgggccgcca ggaaacggat cagatcgtac tcccgctgag acagctgaag
      241 gggctgcccg tccttgagca ccgccatctg gtccgtgtcc actgtcaccc ggcccagctc
      301 catccggttg gcggaggctg ccgccccctt cccagcgggc accatgctca tacggcggat
      361 attggctttc acccgggcca gcagctcccg catggagaat ggctttgtga tgtagtcgtc
      421 cgcccccagc tccaggccca ggaccttgtc ggtctcctcc tcccgggcgg tgagcatgat
      481 aataggggtt gaggagccgc tctcccgcac ctggcggcac acctcaaagc cgtccatccc
      541 gggcagcatc aggtccagca ggatcaggtc cgggtcctgc tccagcgcca gttggagccc
      601 agtggggccg tcgtaggcct ccagggtgtc atacccctcc cggctcaggt tgaaggacaa
      661 aatatccacg atgttctgct catcctctac gatcaggatg ctctttccca cagaaatccc
      721 tccttgtcgt ccatatcagc ggcccagcag agtcttgacc ttcagcacgg tggccactgt
      781 cttcgcgtct gtgatctccc cggacatgac ctgctccacc aatgtgtcaa agggcatggt
      841 caccacatcc agaaactcgt cctcgtccaa gtgctgccgg gcgcgggtca ggtcccgcgc
      901 cagatacatg tgcagcgcct cgtcgcagaa tccaggtgag gcatagatat ggcccagata
      961 ggtcagttcc cccgcctcca ggccggtctc ctcgctcagc tcccgaaggg cggcggggcg
     1021 gtggtcctcc cccgggtcca gcttgccggc gggcagctcc agcagcagac cgtggagcgg
     1081 atagcggtac tgccgcacca gcgccacgtt gtccgcctca tccaatgcca aaacggccac
     1141 gccccctggg tggagcacca cctcccgctg ggcggtcccc ccgtccggca ggccggcggt
     1201 atccagcagg accgtgatga tccggccctg gaacatcgtt ttgctttcaa tggtgcgctc
     1261 tgtcaggtcc attttccgct ccctccccgg cgcgtcccat ttggggcggc ctgcttcaaa
     1321 ttcagggcca ttataccacg cttttccccg tttgccaact aaaagcggtc agccggaaaa
     1381 aagccccggc cgcctgacgg ccggggcccg ccggtggcag gcgccaccgg ctaccggtcc
     1441 cgcctccctc tctgccagag cagcagctcc gccccgaaga ggatggtcac cgctcccgcc
     1501 aggagcaagc tgtaccggtc cggttccatg tgggggagca cccccgcggc gcacagggcc
     1561 agaaagcccc accacaccgc cagcatcaca gcggcggccg ccagctcctc cttctctgcc
     1621 tgccaccagt gcgctccggc ggggatcgcc tggccgcgtc cccggctctc ccaccacgtc
     1681 ttcccccaca ggatggctag ttccgcggtc agcaagccca gccctaccca gaggatcccg
     1741 ggcacctccc ggtctgggag gatgccccag gcgcacagga ccagcagcag ccagcccccg
     1801 ccgagccaca gtcccacccg gatcaggccg tgccgggtaa tctccttctg tctcttctcc
     1861 tgctccatgg ggctcctccc tcttctgtct agcggccttc tccggcccgg ctctgggcgt
     1921 cctggcggat cagcagcttc agcgcctcca cccctacgtc gatggccagc gcggtgtcgt
     1981 gggactcctg ctggtccagc cccgcctgct cccgctcctg attccagatc acgtggaaca
     2041 cagagccgca ccgcactccc cgggccgctg ccacggtgaa cagagccgca gactccatct
     2101 ctgaggccag cacccccagc cgcttccatg cctcccactt cgcctccagc tccccagata
     2161 ccggcatccg ggcggggtcg tgctgtccgt aaaaggagtc cttgcactgg accacccccg
     2221 cgtgccaggg cttccccagc gcctggcagg cctgcaccag cgcgccggcc acctggaaat
     2281 cagggacagc ggggtactcg atgggggcgt actcccggct ggtcccctcc attcgcaccg
     2341 cgccggtggc caccaccacg tctccgctgc ggacatccag gcggatgccg ccgcaggtac
     2401 ccacccggac aaaggtgtgg gtccccaggt tggccagctc ctccatggca atggcggcgg
     2461 aggggccgcc gatgccggtg gagaccacgc tcaccttctc ccccagcagg gagccggtcc
     2521 aggtctcaaa ctcccgattg gtgcgcacat ggaccgcccc ctccagccgc tgggcaatgg
     2581 agcggcatct gcccggatca cccggcagga cacagtagcc ccccacatcc cccggaccgc
     2641 actggatatg aaattgtagt gctctctcct gcatgatctg gccttccttt ccccgcctca
     2701 ggcggacctc cgtttttctt tctatattat atcggttcca ccccacagac gcaagcgtcc
     2761 atccgccggt tttcttttgc cgcctgtacc ccttgcaggg gaatggctca cccctctgcg
     2821 gaaaaaggca ctccgtccga aaacagggat ggctcctggc gcccctgcgg tgccagctcc
     2881 acagcaaaag cccccgcctg ctctggggca ggcgggggcc gcgggctttt ccagcatccg
     2941 gccggtgctt caaaatacca ggtctctccc ggaagtcagt gctttttgaa atagtcgttg
     3001 gtgctgcgaa tgatcacatt ggacagcagc agcaggccaa tgaggttagg gatggccatc
     3061 atgccgttca gggtgtcgga gatatcccac atcagggtgc cgtttccggt ggcacctacg
     3121 atgcacacca gaacaaagac caccttaaag acccacacca ccgctttatt gttgttggac
     3181 agataggccc agcaccgctc accgtagtag ctccagccca ggatggtgga cagggcaaag
     3241 aaaagcaggc tgatctgaat gatggtcccg ccgatggtcc cgggcaggat ggcattgaag
     3301 gcggccaccg ccgccgcgcc gttggtggcg tagctgtcca gccccacgtc cagcacaccg
     3361 gatagaatga cggccaggga ggtcagcgtg cagatgacga tggtgtcgat gaacacctca
     3421 aacacgcccc acatcccctg ctccaccggc tcctgggtct cggaggccgc atgggccatg
     3481 ggggcggagc caaggcccgc ctcgttggag aatacgcccc gggcaaatcc ttggcgcata
     3541 gccaccataa tggcatagcc gaatacgccg ccccccaccg cctcaaagct gaaggcgctg
     3601 gtgaagatgg aggccagggc cgccggcact tggtcgatcc gcagcaggat caccgccagc
     3661 cccgccccaa tgtaaaagat ggccatgaag ggcaccatgt aggaggtcac ccggccgatt
     3721 cgctggactc cgcccagcac cacaatgcct accaccaccg ccaggagcac gccggtggtc
     3781 aggccgctca tctcgaacag cccctccatc gccccggcga tctcgctgct ctgggagatg
     3841 ttcccgatcc cgaagcaggc gaagcccgcc accagtgaaa acagcaccgc cagccacttc
     3901 atgcccaggc ccttttcgat gtaatacatg ggcccgccgt agtggacccc gttctcgtca
     3961 acctgccggt acttcagggc cagtacgatc tccgcaaatt tggtgaacat cccaaagaaa
     4021 gccgcgaacc acatccagaa caccgccccc ggccctccgg cgaagatagc cccggtgaca
     4081 ccggcgatgt tgccagtgcc cacggtgccg gccagagcag tggtcactgc ctgaaagggg
     4141 gtgaggttct tggcatcctt gtcccgcttc tcccgcctgc gaaacagtga gcccaccgta
     4201 ttgcgcatga tatacccaaa ttttgcggcc tggatgcacc cggtgcgggc ggtgaggtag
     4261 acgcccgtgc ccaccaggag cagcagcatc accggccccc acacgaagtt gttgacggcc
     4321 gagttgatct tggtgatgat ttcaatcatg gtttcccttc ctttcctgtt tctgggagcg
     4381 tccggcccgg tcagggtaaa aaaagagcgc ggtccgaggc agccgcgctc tattgatctc
     4441 cggaactctc cccgcggggc ctcccatcct tctccgcccc gctcttttga aaggaattat
     4501 aagggttccc gccccaaaat gcaagaaaaa ttttttatcc ttcatttttt ctgtccaggg
     4561 cagaactctg ccgctccctt acagcaggac gaaggtcacg ccgttgttgt gccgctccag
     4621 gtcccggtcc ctccgcaggg tgggacagcg ctgaaaggcg gccagggtgt cggagtcaaa
     4681 gatggagaag aactctccgg ggatgtagcc ccagatctct ccccccgcct tcatccgatc
     4741 caggcgccgc ctggcttcca cccggatgcg ccctcccgcg ccggaggagc cgtagccgtg
     4801 gataagcttc accccggagc accccaggcc ggggctgcgg cgcagctcaa agagcatctg
     4861 acggatagcc tgctcggccc ggggcatgcc ccgctccagg tccacctccc gcaggtcatt
     4921 catccaagtc ccgccccctt gtctcgatga tgtccccatt cagatcccgc tccaggtccc
     4981 gcaccatccg gctcagccgg tggatgatcc acaggtcgct gagcccgtcg tagatcagca
     5041 ccaggccgat cagggtcacc agggcggcga aggtgccgaa ggggttgatg aggatcagcg
     5101 cccccagggc cagggtcagg gcggagaggg ccaggtccag ccaccaccga gccgctccct
     5161 ggcgcatcag ggagaccgag ctctggacat ccagcacgcc gtggaagatg accacagcgc
     5221 cgaacacata ctgcaccagg gcgatcaccg agtcggagct ggtcatcagc cagatcccca
     5281 ggccggagag caccacgccg gggatcaggg tgaaggccga tatgcccccc tcccgcctgc
     5341 ggaccagcca gcccaggata tacacaaggc cgcagatcag cagggccgcc cccagcaggg
     5401 tgcacatcag ccgcacagac cggtcgggcc acaccaccag caccgccccc agcaccatgg
     5461 tgatcaaagc cacgatgacg ctgctgcgcc gctgttcctg caagatggtt ttcataatag
     5521 tcaggcacct ctttctttat tttgcgcccc cagggcttca gccctcccgg cggtagatca
     5581 gccgtccatc cttccaggtg gccagcacgg ggatgtcccg caggctcatg gggtccgcag
     5641 tgagcgggtc ccgctccagc aacaccaggt ccgcccgctt cccctcccgc aggctgccct
     5701 tctccccctc ctcaaaatac tgccaggccg cccggatggt cactgcggcc agggcttcca
     5761 ccacggggat cttctgctcc ggccccagca ccgcgccgga gcgggtcagc cggttcactc
     5821 cgcaccacac cgtctccagc atgtccggcg ggagcaccgg ggtgtcctgg tggaaggtga
     5881 aggggatgtc ccgctccagg g
//
LOCUS       DBMDSJ010000059         5848 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3898_length_5848_cov_1.982252, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000059 DBMDSJ010000000
VERSION     DBMDSJ010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3898_length_5848_cov_1.982252"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            22..906
                     /gene="hflC"
                     /locus_tag="ACZPAQ_02395"
     CDS             22..906
                     /gene="hflC"
                     /locus_tag="ACZPAQ_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013324.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0098797 - plasma membrane protein complex
                     [Evidence IEA];
                     GO_process: GO:0052547 - regulation of peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR0902860.1"
                     /translation="MKGNRIIKWMFALIVAAIIGFFGFVFKVNEGEYAVVTRFGAVRS
                     EVTQAGMYLRLPWPFEDVQIQDARKRYLDSGYLETLTHDKKNVIMQTYTIWSIADPLR
                     YYTSVGDTALAETYLSDLVTSAKNGTMGNYDLSALVSLDEEDIKIDEIEAAMLQEVQS
                     HALEQYGIQVHELRLKRVGLPATNVQSVFTQMQADRQKYIDQLLAEGERDAQIIMSES
                     DAEVAAIVAEGREEAAAINAETEREVAEIYASAHSQDPELYEFLQKLAALENSVDEDT
                     VMIITMDTPPFDVLYGDD"
     gene            922..1872
                     /gene="hflK"
                     /locus_tag="ACZPAQ_02400"
     CDS             922..1872
                     /gene="hflK"
                     /locus_tag="ACZPAQ_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR0902861.1"
                     /translation="MKDSQRFIAIINTAIRYCKWLIVILVALICCSGIRVVNNHEVAV
                     VLRFGRLVGDTREEQVKEPGLLLAFPYVIDEVIKVPVGKVHEVSITTHAASADPTYAE
                     IEDTGYVITGDENIIHIDATLKYQISDPVEYALYNSDPETEINGVVSGVMTSCAASKS
                     VDGLLTDQKAEFGNEVIRQAQAILDGMEMGVSIVSLEFKSIKPPSALQYHFDQVNAAS
                     VEKETRIQQANQYRERVIPEANAAADKLVSDAQVSQHDRINRANDQVAEFYGLFQEYQ
                     QNRDVVYERVFREKVVTIFNQMGGKIVLPEGTNTPQIFLP"
     gene            1885..3801
                     /locus_tag="ACZPAQ_02405"
     CDS             1885..3801
                     /locus_tag="ACZPAQ_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR0902862.1"
                     /translation="MENLTNTAVHLLEDSQGADLTRREKRKLSVEITTAMIALVCLVT
                     GLLYKGIFPEQTAVAGLIYSVGVLVEGLPLLATAIRGFLQRDVTNAMEILVSIAVLAC
                     YITGQHELAILIPVVLSVVHFLEERSIMGGRDAIEGLKQMQATDAVLETEDGEVTVEV
                     QALKRGDIIIVRPGMGLPIDGTVIWGNSNIDQKSLTGEPLPAAVTVGDTVYAGTTNLD
                     GMIKIRVEKEYQDTSFTKIVSLLEEAQSITVPEIRIVDRFMHYYIPLALIVAALTALL
                     SRNISNAIAVLVVSCPCGHMLVSSAPMIAALAVSTKRGILIKNAKFVEQLTNITTVIF
                     DKTGTITRGELSISGFYLQEAQSREELFARGGCVACSSMHPISRSLMKTLEGEGIPYE
                     EGFQVRETAGKGLTGTRGGEEILFGSRHWIESLGYQPEDPHMDTGGGPANWVVYNGRV
                     LGCLMFDDSVRPEAEEVVSRLHEDGMEQTVLLTGDREFAARKVQRQTGIDQVYFHLLP
                     EEKLEHVKRLRQDAHVLAVGDGINDALALAEADVGIAMGAMGSDVAIQSADIALMNND
                     LNNIPFVMSLARSTKSIMYQNIGIAFSVSLIMMILAAVGVIPALAGAFLHNIGAFVIL
                     INSSRILRDSGGEG"
     gene            4257..5378
                     /gene="prfB"
                     /locus_tag="ACZPAQ_02410"
     CDS             4257..5378
                     /gene="prfB"
                     /locus_tag="ACZPAQ_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR0902863.1"
                     /translation="MLQFDEYKVKLNNLAPTLEELGKALDLESAERELDMLQAESAAD
                     GFWNNLEKAQKVQQRIKNLQDKVDKQNKRRREWDDLMALCEMGNEFEDESLLPELEEG
                     FAQLERDMEEARLQTLLTGEYDSSNVILTIHPGAGGTEAQDWAQMLYRMYTRWTERHG
                     FTYQIMDYQEGDEAGIKSATIMVEGENAYGYLRGEHGVHRLVRVSPFDANARRQTSFA
                     SVEVMPDLPEDVEVDIRPEDIEMQVYRASGAGGQHVNKTSSAVRLIHKPTGIVVASQQ
                     ERSQFQNKDNCMKMLRAKLVELQMQEKADKISDLKGVQLKIEWGSQIRSYVFMPYQMV
                     KDNRTAYETSNINGVMDGDLDGFINAYLTAEATGNWAEK"
     gene            5534..>5848
                     /locus_tag="ACZPAQ_02415"
     CDS             5534..>5848
                     /locus_tag="ACZPAQ_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_process: GO:0007007 - inner mitochondrial membrane
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HIR0902864.1"
                     /translation="MQYQITGEPMPVVICQLASGESMITEKGSMVWMSPNIEMQTSAG
                     GIGKAFGRMFSGESIFQNIYTARGGAGMIAFASSFPGSIREVEVMDDRPMVVQKAGFL
                     AAE"
BASE COUNT         1253 a         1696 c         1905 g          994 t
ORIGIN      
        1 cgggtgagga ggggacatga tatgaaaggg aaccgcatca tcaagtggat gttcgccctg
       61 atcgtggcgg ccatcatcgg cttctttggc tttgtgttca aggtaaacga gggggagtac
      121 gcggtggtga cccggttcgg cgcggtccgg tcggaggtga cccaggcagg gatgtacctg
      181 cgcctgccct ggccctttga ggacgtgcag atccaggacg ccaggaagcg gtatctggac
      241 tccggctacc tggagactct gacccatgac aagaaaaatg tcatcatgca gacctacacc
      301 atctggagca tcgccgaccc cctgcggtac tacaccagcg tgggggatac cgccctggcg
      361 gagacctacc tcagcgacct ggtcaccagc gccaaaaatg ggaccatggg caactatgac
      421 ctgtccgctc tggtctccct ggacgaggag gacatcaaga tcgacgagat cgaggccgcc
      481 atgctccagg aggtgcagtc ccacgctctg gaacagtacg gcatccaggt ccatgagctg
      541 cggctgaagc gggtgggcct gccggccacc aacgtacaga gcgtgttcac ccagatgcag
      601 gcggaccggc agaagtacat cgaccaactg ctggcggagg gtgagcggga cgcccagatc
      661 attatgagcg agtcagatgc ggaggtggcc gccatcgtgg ccgagggccg ggaggaggct
      721 gccgccatca acgcggagac ggagcgggag gtggcagaga tctatgcctc ggcccacagc
      781 caggacccag agctgtatga attcctacag aagctggctg ccctggagaa ctcggtggac
      841 gaggacacgg tgatgatcat caccatggac accccgccat tcgacgtgct ctacggcgat
      901 gactgaaggc ggtgagggaa gatgaaggat tctcagcggt tcattgccat catcaacacc
      961 gccatccgct actgcaaatg gctgatcgtc atccttgtgg cgctgatctg ctgctccggc
     1021 atccgggtgg tcaacaacca cgaggtggcg gtggtgctgc ggttcggacg gctggtgggg
     1081 gacacccggg aggagcaggt gaaggagccg ggcctgctgc tggcattccc ctatgtgatc
     1141 gacgaggtaa tcaaggtgcc ggtgggaaag gtgcatgagg tgtccatcac cactcacgcc
     1201 gcctccgctg accccaccta tgcggagatc gaggacaccg gctatgtgat cacaggggac
     1261 gagaatatca tccacatcga tgccaccctg aagtatcaga tctctgaccc ggtggagtac
     1321 gccctgtaca acagcgaccc agagaccgag atcaacggcg tagtcagcgg cgtgatgacc
     1381 tcctgcgcgg cgtccaagag cgtggacggc ctgctcaccg accagaaggc ggagttcgga
     1441 aacgaggtca tccgccaggc ccaggccatc ctggacggga tggagatggg ggtgagcatc
     1501 gtgagcctgg agttcaagag catcaagccc cccagcgccc tgcaatacca cttcgaccaa
     1561 gtaaacgccg cctcggtgga gaaggagacc cgcatccagc aggccaacca gtaccgggag
     1621 cgggtgatcc cggaggccaa cgccgccgcc gataagctgg tgagcgacgc ccaggtgtcg
     1681 cagcacgacc ggatcaaccg ggccaacgac caggtggcgg agttctacgg cctgttccag
     1741 gagtatcagc agaaccggga cgtggtctac gagcgggtgt tccgggaaaa ggtggtcacc
     1801 atcttcaacc agatgggcgg caagatcgtg ctgcccgagg ggaccaacac gccccagatc
     1861 tttctgccct gaggagggac ggctatggag aacctgacga ataccgcggt ccacctgctg
     1921 gaggacagcc agggggccga cctgacccgg cgggagaagc gaaagctctc ggtggagatc
     1981 accaccgcca tgatagccct ggtctgtctg gtcaccggac tgctctataa ggggatcttc
     2041 ccggagcaga cggcggtggc ggggctgatc tattctgtgg gcgtgctggt ggagggcctg
     2101 cccctgctgg cgaccgccat ccggggcttt ctccagcggg acgtgaccaa cgccatggag
     2161 atcctggtct ccatcgccgt gctggcctgc tacatcactg ggcagcacga gctggccatc
     2221 ctgatcccgg tggtgctcag cgtggtccac tttctggagg agcggagcat catgggaggc
     2281 cgggacgcca tcgagggcct taagcagatg caggccaccg acgcggtgct ggaaactgag
     2341 gatggggagg tgacggtgga ggtccaggcc ctaaaacggg gggacatcat cattgtccga
     2401 cctggcatgg gcctgcccat cgatggcacc gtgatctggg gcaattccaa catcgaccag
     2461 aaatccctca ccggtgaacc cctgcccgcc gccgtgaccg taggggatac ggtgtacgcc
     2521 ggcaccacca acctggacgg catgatcaag atccgggtgg agaaggaata tcaggacacc
     2581 tccttcacca agatcgtcag cctgctggag gaggcccaga gcatcaccgt gcctgagatc
     2641 cggatcgtgg accggttcat gcactactac atcccgctgg ccctcatcgt ggccgccctc
     2701 accgctctgc tcagccgaaa tatctccaac gccatcgccg tgctggtggt gagctgtccc
     2761 tgcgggcaca tgctggtcag ctccgccccc atgatcgctg ccctggcggt gtccaccaag
     2821 cggggcatcc tcatcaaaaa cgccaaattc gtggagcagc tcaccaacat caccactgtg
     2881 atctttgaca agacgggcac catcacccgg ggggagcttt ccatcagcgg cttctatctt
     2941 caggaggccc agagcaggga ggagctcttc gccaggggcg gctgcgtggc gtgcagctct
     3001 atgcacccca tctcccgctc cctgatgaag accttagagg gggagggcat cccctatgag
     3061 gaggggttcc aggtccggga gaccgcgggc aaaggactga ccgggacccg gggcggggaa
     3121 gagatcctgt tcggttcacg gcactggatc gagtccctgg gctaccagcc agaggacccc
     3181 catatggaca ccggaggggg tcccgccaac tgggtggtgt acaatgggcg ggtgctgggc
     3241 tgcctcatgt ttgatgactc cgtccgtccg gaggctgagg aggtggtgag ccggctccac
     3301 gaggacggga tggaacagac tgtgctcctc accggcgacc gggagttcgc tgcccggaag
     3361 gtccagcggc agacagggat cgaccaggta tatttccacc tgctgccgga ggagaagctg
     3421 gagcacgtga agcggctgag gcaggacgcc catgtgcttg cagtgggaga cggcatcaac
     3481 gatgccctgg ccctggcaga ggcggacgtg ggcattgcca tgggggccat gggctctgat
     3541 gtggccatcc agagtgccga catcgccctt atgaacaacg acctgaacaa catccccttt
     3601 gtcatgtccc tggccaggag caccaagagc atcatgtatc agaacatcgg catcgccttc
     3661 tccgtcagcc tcattatgat gatcctggcc gccgtggggg tgatccccgc cctggccggg
     3721 gccttcctcc acaatatcgg agcctttgtg atcctgatca acagctcccg catcctccgg
     3781 gacagcggcg gggagggctg aacgcctgcc gcaaaaaatt ttctctctct gggtgtacgg
     3841 tttcgagcac atattccatg gaccgcggca tatactgatg tgtacgccgg tgggaggacc
     3901 accggcgccg cgatcagata cagagggagg aagttctcga tatggaagaa aatcagatga
     3961 tccaagtaca gcgtccgacg cagcctgagc cctggcgcga tctggacgat ctcatctttc
     4021 agggggacgg ctggctggcc agcgaccgct gaagggccgt ctcgctgagc gagacggccc
     4081 ttccatttgt cgaagcagag ggagaacgcc tctgcacggt gggagcggcg gcgcaaaaaa
     4141 acactggaat atccggtttt ttctttactt tccagccgag ttcagatata ataattgggc
     4201 tttgattgga atactgcccg tcagggcggg aatttgatgg aaagagtgag gaccatatgc
     4261 tccaattcga cgaatacaag gtcaagctga acaacctggc tcccaccctg gaggaactgg
     4321 gaaaagccct ggacctggaa tccgccgagc gggaactgga catgctccag gcggagagcg
     4381 ccgccgacgg cttctggaac aacctggaga aggctcagaa ggtgcagcag cgcatcaaga
     4441 atttacagga taaggtggac aagcagaaca agcgccgccg ggagtgggac gacctgatgg
     4501 ccctgtgcga gatgggcaat gagttcgagg acgaatccct gctgcccgag cttgaggagg
     4561 gcttcgccca gctggagcgg gacatggagg aggcgcgctt acagaccctg ctcactgggg
     4621 agtatgacag ctccaatgtg atcctgacca tccaccccgg tgccggcggc actgaggccc
     4681 aggactgggc ccagatgctc taccgcatgt acacccgctg gaccgagcgc cacggcttta
     4741 cctatcagat catggactac caggaggggg acgaggcggg catcaagtcg gctaccatca
     4801 tggtggaggg ggagaacgcc tacggctacc tgagagggga gcacggcgtc caccgtctgg
     4861 ttcgggtatc tccctttgat gccaacgccc ggcggcagac ctccttcgcc tcagtggagg
     4921 tcatgcctga cctgcccgag gatgtggagg tggatatccg ccccgaggac atcgagatgc
     4981 aggtctaccg ggccagcggc gccggcggcc agcacgtcaa caagacctcc tccgctgtgc
     5041 gcctgatcca caagcccacc ggtatcgtgg tggcctctca gcaggagcgc tcccagttcc
     5101 agaacaagga caactgtatg aagatgctcc gggccaaact ggtggagttg cagatgcagg
     5161 agaaggcgga caagatatcc gacctgaagg gggtccagct gaagatcgaa tggggcagcc
     5221 agatccgctc ctatgtgttc atgccctacc agatggtaaa ggacaaccgc accgcctatg
     5281 agaccagcaa catcaacggc gtgatggacg gggacctgga cgggttcatc aacgcctacc
     5341 ttaccgctga ggccaccggg aactgggcgg agaaatgacc aacggaacgg ccggggacag
     5401 aagggcgggc cgcgggagga gacttccgcg gcccgctttt ttatagttgc cgccaaggag
     5461 tggatacgct atactggagg cggcccccag gagtggggaa aagagactga tcgatcagaa
     5521 aggcgggaaa accatgcagt atcaaatcac aggagaaccc atgccggtgg tgatctgcca
     5581 gctggccagc ggagagagca tgatcacaga gaaggggtcc atggtctgga tgagccccaa
     5641 catagagatg cagacctcgg cgggcggcat cggcaaggct ttcgggagga tgttttccgg
     5701 cgagtccata tttcagaata tctataccgc ccggggcggc gcggggatga tcgcctttgc
     5761 ctccagcttc cccggttcca tccgggaggt ggaggtcatg gacgaccgcc cgatggtggt
     5821 gcagaaggcc ggcttcctgg cagcggag
//
LOCUS       DBMDSJ010000060         5845 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3902_length_5845_cov_1.985898, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000060 DBMDSJ010000000
VERSION     DBMDSJ010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_5845_cov_1.985898"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(88..1341)
                     /gene="murA"
                     /locus_tag="ACZPAQ_02420"
     CDS             complement(88..1341)
                     /gene="murA"
                     /locus_tag="ACZPAQ_02420"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR0902865.1"
                     /translation="MSCYQINGGTPLRGRLAVQGAKNSVLPILAAALLAPGESVIHNC
                     PDLSDVTATLDILRLLGCGVSREGDTVALDARELDGTVIPASLMGELRSSVIFLGALL
                     TRMGEVELSYPGGCELGPRPIDLHLGALRQLGAEIREEGGSLLCRRGKEMRGRELCLS
                     LPSVGATENILLAACGCPGRTTLVGAAQEPEIVDLQEFLRAMGAQVEGAGTPVISIEG
                     GLPLHPAEHTVIGDRIAAATYLCAVGAAGGEIVTTGVDPDHLTAVLCALQEAGCRVET
                     GAGEIRLTCYSPLRAIRPVRTAPHPGFPTDAQAILMAALAGGTGSTMFVENIFSSRYH
                     HVDELSRMGADIQVAGRVAVVNGRRLHGARVRGCDLRGGAALVVAALGAEGTSRISGL
                     KYIRRGYDGLDTALRALGAEITTIL"
     gene            complement(1400..2518)
                     /locus_tag="ACZPAQ_02425"
     CDS             complement(1400..2518)
                     /locus_tag="ACZPAQ_02425"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR0902866.1"
                     /translation="MNILFTCGGTAGHVNPAVALARIFQERNPDCKVLFVGADGGMEN
                     HLVPKEGYEIRSVTITNFHRSFAPADIAHNLGTVRNMVRSKQQAARILDEFQPDLVVG
                     TGGYASYPVVKEAARRHIPTAIHESNAVPGLTTKGLSKVVDRVMVGFEESRSHYDHPE
                     KVVVTGTPVRGDFFRYTRQEAREKLGLTDERPLLLSYWGSLGAEVMNRKMSEFIAREC
                     YDGAPFHHIHGAGRDYGWMQEEIGRRGLALEGTGIEVREYIYDMPLVMAAADVVLCRA
                     GASTISELTAIAKPSILVPSPNVTANHQEKNARVLADQGAAVLLLERDCVENTLYDAA
                     DHLLRDRRRRDDMVRALTAMAVPDAGEKIYWTLRALMK"
     gene            complement(2596..3795)
                     /gene="ftsW"
                     /locus_tag="ACZPAQ_02430"
     CDS             complement(2596..3795)
                     /gene="ftsW"
                     /locus_tag="ACZPAQ_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR0902867.1"
                     /translation="MARKPKRDLTVEEQLARGPLDLPFLMLTMLLLGVGLIMMFSASF
                     ATAYYNDGDPLVYIKNQAVYAAAGVVVMFLVSKINYQTFRLLSVPGIVAAILLLVLAL
                     SPLGVELNEVKRWVRIGPIQFQPSEVAKVAVILFFATRLSKRDTEKKKKWSRRTLTGR
                     TLERLDRIGFLELVPYGAILMVIALLMLMEPHMSGTILVLAGGASVLFAAGISLGWFA
                     AGGTLVAGAMWFIITQTPYMTARINLWLDPWAQRQGDGFQTVQSLLAIGSGGLLGLGL
                     GKSRQKFLYVPEPENDFVFPIVAEELGFIGAVVVLLLFSLLIMRGYWLALHARDKFGA
                     LTIVGIITLLAVQVFLNVGVVTNLIPNTGISLPFFSYGGTALMIQLAEMGIVLSISRQ
                     IPAPKND"
     gene            complement(3866..4852)
                     /gene="mraY"
                     /locus_tag="ACZPAQ_02435"
     CDS             complement(3866..4852)
                     /gene="mraY"
                     /locus_tag="ACZPAQ_02435"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR0902868.1"
                     /translation="MSYILSFIVAFGVTFIAGQILVPLLRKWKAGQSIREDGPTWHMS
                     KQGTPTMGGLMFILGIGVAIVTEGWEEIQRGNFIYLFVYLFALVFGAIGFVDDFQKLR
                     HHANEGLTALQKFLLQLAAAVAFTVLLRNYGYLTPSLYIPFFNVELAMPWVVYMIFAA
                     FVMVATVNAVNLTDGIDGLATGVTIPVALFYVAVSAWFNRSDLTVVSAALAGGLSAFL
                     IYNFHPAKVFMGDTGSLFLGGAVCGLAFALDVPLVIPLVGLIYVLETLSDIIQVIYFK
                     ATHGKRFFRMAPLHHHLELGGWSETKLFCVFSGATLVLCVLAFLGVMNRYPM"
     gene            complement(4917..>5845)
                     /locus_tag="ACZPAQ_02440"
     CDS             complement(4917..>5845)
                     /locus_tag="ACZPAQ_02440"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR0902869.1"
                     /translation="TNLTQDHLDYHGTMEAYRRAKGRLFRQSERAVLNLDDEAGRWYR
                     ERIACPSLTYSENKDTADLTAKNIRLFPGHVEFEAVARGQITRVHLPIPGGFTIYNAL
                     AALACGLCLDIPLDKAAAPLRAVRGVKGRVEVVPVPTAYTVIIDYAHTPDALENILTT
                     ARDFTAGRLICVFGCGGDRDRGKRPLMGAVAAELADLVVVTSDNPRTEGPEDIIEDIL
                     PGMEGMDTQCHVEPDRPEAICWALSQARPGDVVVLAGKGHETYQEINGVQYHLDEREV
                     VAGYFRGQREGAGKKQGQGTENGGKVPAYVVK"
BASE COUNT         1077 a         1971 c         1781 g         1016 t
ORIGIN      
        1 cggtttctcc ttctccctcg tttgcttctg gatgacatgg gtctctccgt tcctccgggc
       61 cctccgcagc gggtgcccgg ccttctctta caaaatcgtg gtgatctccg cccccagggc
      121 gcgcagggcg gtatccagtc cgtcatagcc ccgccggatg tacttcagcc ccgagattcg
      181 ggaggtcccc tccgccccca aggcggccac taccagcgca gcgccgcccc gcaggtcaca
      241 gccccggacg cgtgccccgt ggagcctgcg cccgttgacc actgccaccc ggccggctac
      301 ctggatgtca gcccccatac gggacagctc atctacgtgg tggtaacggc tggagaagat
      361 gttctccaca aacatagtgc tcccagtccc gccggccagg gcggccatga ggatggcctg
      421 ggcatcggtg gggaagcccg ggtggggggc ggtgcgcact gggcggatag ccctcaaggg
      481 gctgtagcag gtcagacgga tctcccccgc cccggtctcc acccggcagc ccgcctcctg
      541 gagtgcgcag agcaccgctg tcaggtggtc cgggtccact cccgtggtca cgatctcgcc
      601 ccccgcggct cctactgcgc acagataggt cgcagcggca attcggtctc cgattacggt
      661 atgttccgcg gggtggaggg gcagtccgcc ctctatggag atgaccggcg tccccgcccc
      721 ctccacctgt gcacccatgg ccctcaggaa ctcctgaagg tctacgatct ccggctcctg
      781 ggcggctccc accagagtgg tccggccagg acagccacag gcggccagca ggatgttctc
      841 tgtggccccc acgctgggca gggagaggca cagctcccgc ccccgcatct cctttccccg
      901 ccggcacagg aggctgcccc cctcctcccg gatctccgcc cccagctggc gcagcgcgcc
      961 cagatggagg tcgatgggcc gcgggcccag ttcgcagccc ccagggtagg acagctccac
     1021 ctcccccatc cgggtcagca gcgcccccag gaagatcaca gaggaccgca gctcccccat
     1081 cagggaagcg gggatcaccg tcccatccaa ctccctcgcg tccagcgcga cggtgtcccc
     1141 ctccctgctc accccgcagc ccagcaggcg caggatgtcc agggtagcgg tcacgtcgga
     1201 caggtccgga cagttgtgga tcacgctctc tccgggggcc agcagggcgg cggccaggat
     1261 gggcagcaca ctgttcttgg ccccttgaac agcgagccgc ccccgcaggg gggttccgcc
     1321 gttgatttga taacagctca tggaatggcc tccaatcctt atggattcag gtcattctat
     1381 ggaaaaaagc ggatcttggt tacttcatca gggcccggag ggtccagtag atcttctccc
     1441 ctgcgtcggg caccgccata gccgtcagtg cccggaccat gtcatcccgg cggcggcggt
     1501 cccgcagcag atggtccgct gcgtcataga gggtgttctc cacacagtcc cgctccagca
     1561 gcagcaccgc ggccccctgg tcggccagca cccgggcgtt cttctcctgg tggttggcgg
     1621 tgacgttggg ggaggggacc agaatcgagg gcttggcgat ggcggtgagc tccgagatgg
     1681 tggaggcgcc tgcccgacac agcaccacat cggcggcggc catcaccagg ggcatatcat
     1741 agatgtactc ccgaacctcg atgcccgtgc cctccagagc caggccccgg cggccaatct
     1801 cctcctgcat ccagccatag tcccgcccgg ccccgtggat gtggtggaag ggggctccgt
     1861 catagcattc acgggcaatg aactcgctca tcttccggtt catcacctcc gcccccaggg
     1921 aaccccagta ggacagcaga agaggccgct catcggtcaa ccccagcttt tccctggcct
     1981 cctgccgggt atagcggaaa aagtcgcccc gcaccggggt tccggtgacc accaccttct
     2041 ccgggtggtc atagtggctc cggctctcct caaagcccac catcacccgg tccaccacct
     2101 tggacaatcc cttggtggtc aggccgggca cggcgttgga ctcgtggatg gcggtgggga
     2161 tgtgccgccg ggcggcctcc ttcactacgg ggtaggaggc gtagcccccg gtacccacca
     2221 ccagatccgg ctgaaattcg tccaggatgc gggccgcctg ctgcttggat cggaccatgt
     2281 tccgcaccgt gcccaggttg tgggcgatat ccgccggcgc gaaggagcgg tgaaaattgg
     2341 tgatggtcac cgagcggatc tcatagccct ctttggggac caggtggttt tccatcccgc
     2401 catccgctcc cacaaacagg accttgcagt ccgggttccg ctcctggaag atccgggcca
     2461 gcgccaccgc cggattgaca tgtcccgccg tgccgccgca ggtaaaaagg atattcatgg
     2521 tatcactcca ctctctcgtt taggccgaag gcgcgcgctc cgacggggct gccccgccgc
     2581 ctgtcttcct ccggatcagt catttttggg tgcagggatc tgtctggata tgctcaatac
     2641 gatccccatc tccgccagct ggatcatcag cgctgttcct ccgtagctga agaaggggag
     2701 ggagattccg gtgttgggga tcagattggt gaccacgccc acgttcagga aaacttggac
     2761 cgccagcagg gtgataatgc ccacgatggt cagtgctccg aatttgtccc gtgcgtggag
     2821 tgccagccag tacccccgca tgatcagcag ggagaacagg agcagcacca ccaccgcccc
     2881 gatgaatccc agctcttccg ccacgatggg gaatacaaag tcgttttccg gctcggggac
     2941 atagaggaat ttctgtcggc tcttgcccag ccccaggccc agcaggccgc cggagccgat
     3001 ggccagcagg gactgcaccg tctgaaagcc gtccccctgc cgctgggccc atgggtccag
     3061 ccacagattg atgcgggccg tcatataggg ggtctgggtg atgatgaacc acatggctcc
     3121 cgccaccagg gtcccgccgg cggcgaacca gcccaggctg atcccggcgg cgaagaggac
     3181 cgaggctccc cccgccagca ctaggatggt gcccgacatg tggggctcca tcagcatcag
     3241 cagggcgatc accatcagga tggccccata gggcaccagc tccagaaagc cgatgcggtc
     3301 cagccgctcc agcgtccgac cggtgagggt gcggcggctc cacttcttct ttttctctgt
     3361 gtcccgcttg gacaggcggg tggcaaaaaa caggatcacc gccaccttgg ccacctccga
     3421 gggctggaac tggatagggc cgatgcgcac ccagcgcttc acctcattga gctccactcc
     3481 caggggactg agggccagca ccagcagaag gatagcggcc acgatgccag gtacggacag
     3541 cagtcggaaa gtctggtagt tgatcttgga gacaaggaac atgaccacta ccccagccgc
     3601 ggcatagacc gcctggtttt tgatatatac cagcgggtcg ccgtcgttgt aataggccgt
     3661 ggcaaaggag gcggagaaca tcatgatgag tcccacgccc aatagaagca tggtcagcat
     3721 gagaaagggc agatccagcg gcccccgggc cagctgttcc tccaccgtga ggtcgcgctt
     3781 tggttttcgg gccacgagga ccaccccctg ttctgagata ctgtgacagt gacccgccgg
     3841 agagaaaagc gctccgcccc gcccgtcaca tgggatatcg gttcatgacg cccaggaagg
     3901 ccagcacgca gaggaccagg gtggcgccgg agaaaacgca gaacagcttg gtctcgctcc
     3961 agccccccag ctccaggtgg tgatgaaggg gcgccatccg gaaaaagcgt ttgccgtggg
     4021 tcgctttgaa gtagatcacc tggatgatat cagagagagt ctccagcaca tagatcagtc
     4081 ccaccagagg gataaccagg gggacgtcca gggcaaaggc cagaccgcac accgcccccc
     4141 ccaggaacag ggagccggtg tcccccatga acaccttggc tggatggaag ttatagatca
     4201 gaaaggcgga caggccgccc gccagggccg cagacaccac cgtcaggtcg ctgcggttga
     4261 accaggcaga caccgccaca tagaagaggg cgacggggat ggtgaccccg gtggccagcc
     4321 cgtcgatgcc gtccgtcagg ttcactgcgt tgacggtagc caccatgacg aaggcggcga
     4381 agatcatata gaccacccat ggcatggcca gctccacatt aaaaaagggg atgtacagac
     4441 tcggggtcag atatccgtag ttgcgcagca gcacggtgaa cgccaccgcc gccgccagct
     4501 ggagaagaaa tttctgcagg gccgtcagcc cctcgttggc gtgatggcgc agcttttgaa
     4561 agtcatccac aaagccaatg gctccaaaca ccagagcgaa gaggtacacg aagagataga
     4621 taaagttccc ccgctggatc tcttcccagc cctcggtcac aatggccacc ccgatcccca
     4681 ggataaacat cagtccgccc atggtgggcg ttccctgctt ggacatgtgc caggtgggtc
     4741 cgtcctcccg gatgctctgc cccgctttcc acttgcgcag cagggggacc aagatctgtc
     4801 ccgcaataaa ggtcactcca aaggccacga taaagctgag aatatatgac atagctgcca
     4861 ccctccaggc attctttctc ttgggtatca cacacaggtc tgtgctcaat ggcgtattat
     4921 tttaccacat acgcagggac ttttccaccg ttttctgtcc cctgtccctg cttttttccc
     4981 gcgccttccc gctggccgcg gaaatagccg gccacgactt cccgctcgtc caagtggtac
     5041 tggacgccgt tgatctcctg ataggtctca tgcccctttc cagccagcac gaccacgtcc
     5101 cccgggcgcg cctgggacag ggcccagcag atggcctccg gacggtcggg ctccacgtgg
     5161 cactgggtgt ccatcccctc catgcccggc aggatatcct cgatgatatc ctccggcccc
     5221 tcggtgcgtg ggttgtcaga ggtgaccacc accaggtcag ccagctcggc ggccaccgcc
     5281 cccatgaggg ggcgcttgcc ccggtcccgg tctccgccgc agccgaacac acagatcagc
     5341 cggcccgcag tgaagtcccg ggcggtggta aggatgttct ccagggcatc cggcgtgtgg
     5401 gcatagtcga tgatgacggt gtaggccgtg gggacaggca ccacctccac ccggcccttc
     5461 accccccgca ccgcacgcag gggggcggcc gccttgtcca gcgggatgtc caggcagagc
     5521 ccgcatgcca gcgccgccag ggcgttgtag atggtaaagc cgccggggat gggcagatgg
     5581 acccgggtga tctgcccccg ggccaccgcc tcgaactcca cgtggccggg aaagaggcgg
     5641 atgttcttag cggtcaggtc cgccgtgtcc ttgttctcag agtaggtcag ggaggggcag
     5701 gcgatccgtt cccggtacca ccggcccgcc tcgtcgtcca ggttgagcac cgcacgctcc
     5761 gactggcgga acagcctccc cttagcccgc cggtaggcct ccatggtccc gtggtagtcc
     5821 agatggtcct gggtcaggtt ggtga
//
LOCUS       DBMDSJ010000061         5843 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3903_length_5843_cov_1.859108, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000061 DBMDSJ010000000
VERSION     DBMDSJ010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_5843_cov_1.859108"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="ACZPAQ_02445"
     CDS             complement(<1..663)
                     /locus_tag="ACZPAQ_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR0902870.1"
                     /translation="MSEEVKNEFTFENPEYRQTYWHTCSHVMAQAVKRLWPEVKLAIG
                     PSIDEGWYYDMDAPFAFTPEHLEQIEAEMRKICKEKLKLERFELPREEAVKFMEEKGE
                     PFKVELIQDLPEDAHISFYKQGEFTDLCAGPHLDSTGRIKGNALKLTACNAAYWRGDS
                     NRETLQRIYGIAFPKKDELDAYLAKIEEAKKRDHRKLGKELGLFMLRDEGPGFPFFLP
                     KGM"
     gene            complement(1041..1361)
                     /locus_tag="ACZPAQ_02450"
     CDS             complement(1041..1361)
                     /locus_tag="ACZPAQ_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902871.1"
                     /translation="MRRVDLARPGAPSGDRARVARLTRHLKARLEDFGPGGPQVTGVD
                     EASGTVTARFPGQDTGGVLDRLERDWGVTVLQDGEDAVFRLAPSVRFEDLDYVWGALF
                     EVLG"
     gene            complement(1358..2548)
                     /locus_tag="ACZPAQ_02455"
     CDS             complement(1358..2548)
                     /locus_tag="ACZPAQ_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR0902872.1"
                     /translation="MKKRLGALLCALVMCLALLSPASALEEVCFTSLNNKLLPLASDT
                     MPLWSGGVLYVPASLFDGSATNASYGVTLGVSLTQSQSSGTITLYSLSGILVFDLNSG
                     ICVDQQTGELLSGRAITRNGRIYLPLNIVVDFFGLRSSYLHTDYGYLVRIKNRDDTAS
                     NGRRSILTDDVFIDSAGNQMNLQLKEYQKSLLPVEEPETPDVTPAVPADPPGQELDPT
                     SIPTYLAFRCQTGEALDSILTTLEKSGKSGVFFFAPEELSVHGDMVRRLLGTGHSVGI
                     LVQAGTPEGARAELEAGNRALAEQTRSCTRYVYCGEALWEGLEAEGWVCWQESLDAVP
                     LDGEQSAASYSYGVLRQLGRAALNSAYVTLDDSRQTADVLPALLRQLNYREYVVSTPM
                     ETKL"
     gene            2726..3361
                     /locus_tag="ACZPAQ_02460"
     CDS             2726..3361
                     /locus_tag="ACZPAQ_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902873.1"
                     /translation="MEITVEMVERLREKAPVSYAQAKEALEYSEGNLLDALIYLEEKG
                     AIPQAEGTRWSGREEKESQPTAAELVVQETPEPGKKKKHRSPLGVVVVWPKGEKRRGR
                     SLRELLSRLYRVLVDNELEVWRRDEPVTSLPLLILLILFVFAFYVTIPLLLIGLFFGF
                     RYRFSGPELERDSINDVMENVASTAADMGRQVMDELHLQYEKRTRDRDGKE"
     gene            3402..4088
                     /locus_tag="ACZPAQ_02465"
     CDS             3402..4088
                     /locus_tag="ACZPAQ_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0902874.1"
                     /translation="MEEKILLVEDEEKLARMVELELRYEGYQVDKSFDGRSGLERALS
                     GEYDLVLLDIMLPQLSGMEVLRRLRKEGVQLPVIMLTARDSVVDKVSGLDSGADDYIT
                     KPFAIEELLARIRAALRKRGSGERPEEPLLSAGPLTMDVERHTVAVSGTPVELTRREF
                     DLLHHLLENKGRVLSREALLDSVWGFDFVGETNSVDVYIRFLRSKIDEAFDIKLIHTV
                     RGVGYVIREE"
     gene            4116..4622
                     /locus_tag="ACZPAQ_02470"
     CDS             4116..4622
                     /locus_tag="ACZPAQ_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902875.1"
                     /translation="MTIVYTSKTGFTREYAQMLGRAAGLPVCALDEATGKVGPGSDIL
                     YLGWLMAGHISGIDHAVKRFHVRAACGVGMTPPGDKVLQDLARSNYVPNAPIFYLQGG
                     WAPKKVGWLQRRMVGMATRGLRQSLQQKRERTPQEQRQLDLLLRGGSCVAFRNLTPLL
                     EWLKKQEG"
     gene            4657..>5843
                     /locus_tag="ACZPAQ_02475"
     CDS             4657..>5843
                     /locus_tag="ACZPAQ_02475"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902876.1"
                     /translation="MGKRDMKNKTPPSGAKSQSLVLRLNTRLFFRQLGIFLCMDLLLV
                     VLALMGQLMWAEKRCADVAQLVQERGVPTAEAVPWMAASDYTIIPLDREPKGVDVGEL
                     GWFSGGEATADGLRTYVLFSYYTVELPNGGEPYAITVDLSGIGRMLKWAGFILLTVQI
                     ISLIANLFRNAGTIRRTLRPIQELAATAARLNSMTHMSRRELEALAGELSKINATHLD
                     SRIDLPGTQKELKTLAKAINDMLDRVNAAYSAQMQFVSDASHELRTPIAVIQGYAALL
                     DRWGKDDPEARQEAIDAIRSESDAMERLVEQLLFLARGDNDTQPIRPQLMDLTAVAED
                     VLREEQMIHPERAFLARWQGPVVVRADPGLVKQVMRILMDNSVKYSGPEGRVYLRVGP
                     DHHR"
BASE COUNT         1059 a         1769 c         2042 g          973 t
ORIGIN      
        1 catgcccttg ggcaggaaga aggggaagcc ggggccctcg tcccggagca tgaacaggcc
       61 cagctccttg cccagcttcc ggtggtcccg cttcttggcc tcttcgatct tggccagata
      121 ggcgtccagc tcgtccttct tggggaaggc gatgccgtag atgcgctgga gggtctcccg
      181 gttggagtca ccgcgccagt aggcggcatt gcaggcagtg agcttcaggg cgttgccctt
      241 gatgcggccg gtggagtcca ggtggggacc ggcgcacagg tcggtgaact cgccctgctt
      301 atagaaggag atgtgggcgt cttcgggcag atcctggatc agctccacct tgaagggctc
      361 tcccttctcc tccataaatt tgacagcctc ctcccggggc agctcgaacc gctccagctt
      421 cagcttctcc ttgcagatct tccgcatctc ggcctcgatc tgctccaggt gctcgggcgt
      481 gaaggcgaag ggggcgtcca tgtcgtagta ccagccctcg tcgatagagg ggccgatggc
      541 cagcttcacc tcgggccaca ggcgcttgac cgcctgggcc atcacatggg agcaggtgtg
      601 ccagtaggtc tggcggtact cagggttttc aaaggtaaat tcatttttga cttcttcgga
      661 catcaaaata cctccttgcg tccgcggggg tgcgcagggc gcacccccaa aaggctctga
      721 ggggcaaaac aaaagcgcct cgcccctgaa tctccatcag gggtgaggcg cagtaaggcg
      781 ctccacggtt ccaccctgct tgcggtttta cagaaaaccg ccgcttgtga ccgctgtaac
      841 gggcgggccc gtcccggtca tcccgggcgg ctcaggagtg gtaccagccg cggcaatgtc
      901 gcaggacgct tccagccttt gcgtccctct ctgggcgagt cccgcggcct cttctccatc
      961 agtgctcatt tgacaaacgc actatatcac caaacgcccg acctgtcaag tggggaatgg
     1021 aagaaggaaa ggagccgatc tcagcccagc acctcgaaga gcgcgcccca cacgtagtcc
     1081 aggtcctcaa accggacaga gggggcaagg cggaacacgg cgtcctcccc atcctgaagg
     1141 acggtgacac cccagtcccg ctccagccgg tccagcaccc cgccggtgtc ctggccgggg
     1201 aagcgggcgg taacggtgcc ggacgcctca tccacccccg tcacttgggg cccgccgggg
     1261 ccgaagtcct ccagccgggc cttcaagtgc cgggtcaggc gggccacccg ggcccggtca
     1321 ccagaggggg ctccgggccg ggccagatcc acccgcctca cagcttggtc tccatcgggg
     1381 tactgaccac gtactcccga tagttcagct gacgaagcag ggcggggaga acatccgcgg
     1441 tctgccggct gtcatccagg gtaacatagg cgctgttcag ggcagcccgg cccagctggc
     1501 gcagaacgcc gtaggagtag gaggcggcgc tctgctcccc gtccagagga acggcgtcca
     1561 ggctctcctg ccagcagacc cagccctccg cctccagccc ctcccacagg gcctctccgc
     1621 agtagacata gcgggtgcag ctgcgggtct gctccgccag ggcgcggttc cctgcctcca
     1681 gctccgctcg ggccccctcc ggggtgccgg cctggacaag gatgccaacg ctgtgcccgg
     1741 tgcccagcag gcggcggacc atgtccccat ggacagagag ctcctccggg gcgaagaaga
     1801 agacgccgga ttttccggat ttctccaaag tggtgaggat gctgtccaac gcctcccccg
     1861 tctggcagcg gaaggccaga taggtgggga tggaggtggg gtccagctcc tggcccgggg
     1921 gatcggcggg gaccgctggg gtgacgtcag gggtctcggg ttcctccaca gggagcaggc
     1981 tcttttggta ctccttcagc tggaggttca tctggttgcc ggccgagtcg atgaagacat
     2041 cgtcggtgag gatggagcgc cgcccgttgg aggcggtgtc gtcccggttc ttgatgcgga
     2101 ccagatagcc gtaatcggtg tggagatagg agctccgcag cccgaaaaag tccaccacga
     2161 tgttcagggg gaggtagatg cggccgttgc gggtgatggc ccggccgctg agcagctcgc
     2221 cggtctgctg gtctacgcag atgccggagt tcaggtcaaa gaccaggatg ccgctgaggc
     2281 tgtaaagggt gatggtgccg ctgctctggc tctgagtgag gctgaccccc agggtcacgc
     2341 cgtagctggc gttggtggcg ctgccgtcga acagggaggc gggcacgtag aggacgcctc
     2401 cggaccagag gggcatggtg tcggaggcca ggggcagcag cttgttgttc agggaggtaa
     2461 agcacacctc ctccaaagcc gaggcggggg acagcagggc cagacacatg accagggcgc
     2521 acagcagggc gccgagacgc tttttcaagg ggcacacctc cgtttccagt tgataagggg
     2581 attatagcat agtataatga gaatcacaag cagccgcggg aaaggcgttt tcatccattt
     2641 ttaagaaagg ctaaaggaca cttcaagccc gggcggttat actgacgtca gaacaggcag
     2701 actgcccgga aaggagcgat tcaggatgga gatcacagtg gagatggtag agcgcctgcg
     2761 ggagaaggcc cccgtgtcct acgcccaggc caaggaggcc ctggagtaca gcgaggggaa
     2821 tctgctggac gccctgatct atctggagga gaagggggcc atcccccagg cggaggggac
     2881 ccggtggtcg ggccgggagg agaaggagtc ccagcccacg gcggccgaac tggtggtcca
     2941 ggagacaccg gagccgggaa agaaaaagaa gcacaggagc ccgctgggcg tggtggtggt
     3001 gtggcccaag ggggagaagc ggcggggccg ctccctgcgg gagctgctgt cccggctcta
     3061 ccgggtgctg gtagataacg agctggaggt gtggcgccgg gacgagccgg tgacctccct
     3121 gcccctgctg atcctgttga tcctgtttgt gttcgccttc tatgtgacca ttcccctgct
     3181 gctgatcggg ctgttcttcg gattccgcta ccgcttttcc ggccccgagc tggagcggga
     3241 cagcatcaac gacgtgatgg agaacgtggc cagcaccgcc gccgacatgg gacgccaggt
     3301 aatggatgag ctgcacctcc agtatgagaa gcggacccgg gaccgggacg gcaaggaata
     3361 gatctccggg ggagtgcccg gatgttgagg tgagagagga tatggaagaa aagatccttc
     3421 tggtggagga cgaggaaaag ctggctcgga tggtggagct ggagctgcgg tatgagggct
     3481 atcaggtgga caagagcttc gacggccgct ccggcctgga gcgggccctg tcgggggagt
     3541 acgacctggt gctgctggac atcatgctgc cccagctgtc cggcatggag gtgctgcgcc
     3601 gcctgcggaa ggagggggta cagctccccg tcatcatgct caccgcccgg gacagcgtgg
     3661 tggacaaggt atccggcctg gactccgggg cggacgacta tatcaccaag cccttcgcca
     3721 tcgaggagct gctggcccgc atccgggcgg ccctgcggaa gcgggggagc ggagagcggc
     3781 cggaggagcc gctgctgtcc gccggtcccc tgaccatgga cgtggagcgc cacaccgtgg
     3841 cagtgtccgg gacgccggtg gagctgaccc gccgggagtt cgacctgctc caccacctgc
     3901 tggagaacaa gggcagggtg ctcagccggg aggccctgct ggacagcgtg tggggcttcg
     3961 acttcgtggg ggagaccaac tcggtggacg tatatatccg tttcctccgc agcaagatcg
     4021 acgaggcctt tgacatcaag ctgatccaca ctgtacgggg cgtgggctac gtcatccggg
     4081 aggagtaagg ccgggaagaa ggggagctga ttcatatgac gatcgtttat acctccaaga
     4141 ccggttttac ccgggagtac gcccagatgc tgggccgggc cgccgggctg ccggtctgtg
     4201 ccctggacga ggctacggga aaggtggggc ccgggagcga catcctgtac ctgggatggc
     4261 tgatggccgg gcatatcagc ggcatcgacc acgcagttaa gcgctttcat gtccgggccg
     4321 cctgcggcgt gggcatgacg ccgccggggg acaaggtgct gcaggacctg gcccggtcca
     4381 actatgtgcc aaacgccccc atcttctacc ttcagggcgg ctgggcaccg aaaaaggtgg
     4441 ggtggctcca gcgccggatg gtagggatgg ccaccagggg gctgcggcag tccctccagc
     4501 agaagcggga gcggacccct caggaacagc ggcagctgga tctgctgctc cgcgggggca
     4561 gctgcgtggc cttccggaat ctgacgcccc tgctggagtg gctgaaaaag caggagggct
     4621 gaccggctgc ggcatggaag gagaggaggg ggcccggtgg gaaaacggga catgaagaac
     4681 aagactcccc ccagcggggc caagtcccag tcgctggtcc tgcggctgaa tacccggctg
     4741 tttttccggc agctggggat ctttctgtgt atggacctgc tgctggtggt gctggccctg
     4801 atggggcagc tgatgtgggc ggaaaagcgc tgcgcggacg tggcccagct ggtccaggag
     4861 cggggggtgc ccaccgcgga ggccgtcccc tggatggctg ccagcgacta caccatcatc
     4921 ccactggacc gggagcccaa gggagtggac gtaggggagc tggggtggtt ctctggcggg
     4981 gaggcaacag cggacggcct gcgcacctat gtgctgttct cctactacac cgtggaactg
     5041 cccaacggcg gggagcccta cgccattacg gtggatctgt ccgggatcgg gcggatgttg
     5101 aagtgggcgg gctttatcct cctcacggtg cagatcatct ccctgattgc caacctgttc
     5161 cgcaatgccg ggactatccg ccgcaccctg aggcccatcc aggagctggc ggccactgcc
     5221 gcccggctca actccatgac ccacatgtcc aggcgggagc tggaggccct ggccggcgag
     5281 ctgagcaaga tcaacgccac ccacctggac agccgcatcg acctgcccgg cacccagaag
     5341 gagctgaaga ccctggccaa ggccatcaac gacatgctgg accgggtgaa cgcggcctac
     5401 tccgcgcaga tgcagttcgt ctctgacgcc agccacgagc tgcgtacccc catcgcggtc
     5461 attcagggtt acgccgctct gctggaccgg tggggcaagg acgacccgga ggcccggcag
     5521 gaggccatcg acgccatccg ctccgagtcg gacgccatgg agcgtctggt ggagcagctg
     5581 ctgttcctgg cccggggaga caacgacacc cagcccatcc ggccgcagct gatggacctg
     5641 accgcggtgg cggaggacgt gctgcgggag gagcagatga tccacccgga gcgggccttc
     5701 ctggcccggt ggcaggggcc ggtggtggtc cgggccgacc cggggctggt gaagcaggtg
     5761 atgcgcatcc tgatggacaa cagcgtcaag tacagcggcc cggaggggcg ggtctatctc
     5821 cgggtcggcc cggaccacca ccg
//
LOCUS       DBMDSJ010000062         5730 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3979_length_5730_cov_1.928939, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000062 DBMDSJ010000000
VERSION     DBMDSJ010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3979_length_5730_cov_1.928939"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="ACZPAQ_02480"
     CDS             complement(<1..1700)
                     /locus_tag="ACZPAQ_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR0902877.1"
                     /translation="MYIADLHIHSRFSRATSRDCDLPHLDWWARRKGIQLIGTGDFTH
                     PAWRAEMREQLVPAGEGVYALREGLTMEGTAPGAAPRFVVTGEISCIYKRHGRTRKVH
                     NLILLPSLEAAEELSVRLEAIGNIHSDGRPILGLDSRDLLELTLETCPEAEFIPAHIW
                     TPHFAMFGAFSGFDTVEECFGDLADQIHGVETGLSSDPPMNWRVSALDRLSLLSHSDA
                     HSPSRLGREADLLDTGLSYPELVQAIRTGEGLLGTLEFFPEEGKYHLDGHRNCGVCLT
                     PAETAERGGLCPVCGKKLTIGVEHRVEELADRPAGFRPEEAKPFESLAPLPEVIAAST
                     GGSAAGKKTLEQYERLLQTLGPEFTILRDVPIEDLQREAGPCVAEGIRRLRLGQVERR
                     PGFDGEYGAISLLAPAEIQRLSGQVSLFGAEETPKQGEKKRGQLPKRPKAAEREGEQG
                     LQRSNSQQQAAISAEERTVAVVAGPGTGKTKTLVDRIAWLVEERGVRPGEITAVTFTN
                     QAAAEMRQRLEERLGRRAASRMTIGTFHAICLALLGDVGLIGQGEALSLAEETLRVLG
                     R"
     gene            complement(1794..2165)
                     /locus_tag="ACZPAQ_02485"
     CDS             complement(1794..2165)
                     /locus_tag="ACZPAQ_02485"
                     /inference="COORDINATES: protein motif:HMM:NF020349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation initiation factor Spo0A C-terminal
                     domain-containing protein"
                     /protein_id="HIR0902878.1"
                     /translation="MNEINVIRRELRALGITRRCRCYGRIVMAIQLAVDQEDSLEAVT
                     KEIYWEVGSRCGRKWTAVERNIRTAVQIAWRTNPELLREMAGYPLDGPPTASEFLEIL
                     SNDVQRAMERREEGVSSTPGI"
     gene            2428..2715
                     /locus_tag="ACZPAQ_02490"
     CDS             2428..2715
                     /locus_tag="ACZPAQ_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HIR0902879.1"
                     /translation="MRTQLWQLACLYWTTANLLAFALMGLDKRRARRGCWRISEKALF
                     FFPVLGGSLGGMLGMYFFHHKTRHWYFRAGFPLLFLFQALLLFFLWRWLAP"
     gene            complement(3043..3564)
                     /locus_tag="ACZPAQ_02495"
     CDS             complement(3043..3564)
                     /locus_tag="ACZPAQ_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR0902880.1"
                     /translation="MIRLATEQDLPLVGSIYEEILAEEEKRPASFTNWQRGKYPTVEH
                     ARAALEAGTLYVAEEDGEVYGCVNLNGEQLAEYAALNWSVPADPEDVMVIHTLCISPR
                     WAGRGKAREFVAFCEEEARRKGKKVMRLDTYEGNLPANTMYPKLGYTFVGGTEFFFQG
                     FIREILNCYDKKL"
     gene            complement(3634..>5730)
                     /gene="ftsH"
                     /locus_tag="ACZPAQ_02500"
     CDS             complement(3634..>5730)
                     /gene="ftsH"
                     /locus_tag="ACZPAQ_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR0902881.1"
                     /translation="LFTMMIKSCTSSYANMGTVEVPYTTFKEWLAEDKIDTVNVENGK
                     YTFTLRDGVEVELPREETSGGSGSLMNSLIPVPAQTEEEKQYITVPLAGVDDPELLAM
                     LEEHADHAYTEPVDESAYFLSLILSYVLPLLLMVGLMMFLFRGIGGKGGMGGLGGVGK
                     ANAKVYVEKKTGVTFRDVAGQDEAKESLQEIIDILHNPQKYTEIGAKLPKGALLVGPP
                     GTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFKEASKMAPCIIFIDE
                     IDTIGKSRDNRMGGNDEREQTLNQLLAELDGFDPGKGVIVLGATNRPEVLDKALLRPG
                     RFDRRITIDRPNLAGRLATLQVHTRKIKLGEDVDLKKIALATAGTVGADLANLVNEAA
                     LRAVRHGRRVVTQEDLLASFEFVIAGSEKKNSVLTEFEKKLVAYHEVGHAMVAYKQKN
                     AEPVQKITIVPHTEGSLGYTLLMPEEDKTNLRTKEELMAKITVSMGGRAAEQVVLNTM
                     TNGASQDIQEATNLARNMVAMFGMSDEFGMMALGSIRNQYLDGGYGLDCAQDTAAVMD
                     KAVKAILDQCYQDAVELIRENLEDMHKVVAYLLEKETITGGEMVAIIQGRDPALVEDA
                     YASTHGRSRSLTGDIEPPAKNIHIVSEPVSAPQLPDGEPEQSGPEEPPAQPRDDETPP
                     PQDGEGPNEKPEQDRP"
BASE COUNT          886 a         1964 c         1677 g         1203 t
ORIGIN      
        1 cggcccagca cccggagggt ctcctccgcc agggacaggg cctccccttg gccgatcagc
       61 cccacatccc ccagcagagc caggcagatg gcatggaagg tcccgatagt catgcgggag
      121 gcggcccgcc gccccaggcg ctcctccagc cgctggcgca tctcagcggc tgcctggttg
      181 gtgaaggtca ccgcagtgat ctcccccggc cggacccccc gctcctccac cagccacgcg
      241 attcggtcca ccagggtctt ggtcttgcct gtgccaggtc cggccaccac cgctacggtt
      301 cgctcctccg ccgagatggc cgcctgctgc tgggaattgg agcgctggag gccctgctcc
      361 ccctcccgct ccgccgcctt cggccgcttt gggagctgtc cccgcttttt ctccccctgt
      421 tttggggtct cctctgcccc aaacagggag acctgtccgc tcaggcgctg gatctccgcc
      481 ggggcaagga gggagatggc accgtattcc ccgtcaaacc cgggacggcg ctccacctgc
      541 cccagccgca ggcgccggat gccctccgcc acgcaggggc ccgcttcccg ctgcaggtcc
      601 tctatgggga cgtcccgcag gatagtgaat tccggcccca gggtctgcag caagcgctca
      661 tactgttcca gggtcttttt cccggcggcc gagccgccgg tggaggcggc gatcacctcc
      721 ggcagggggg ccaggctctc gaagggcttg gcttcctccg gccggaagcc cgccggccgg
      781 tcggccagct cctccacccg gtgctccacc ccgatggtca gctttttccc gcacaccgga
      841 cacagtccgc cccgttccgc cgtctcggcg ggggtgaggc acaccccgca gttccggtgt
      901 ccgtccaggt ggtactttcc ctcctccggg aagaactcca gggtgcccag aagcccctcc
      961 ccggtgcgga tagcctggac cagctccgga taggagaggc cggtgtccag caggtcggcc
     1021 tcccggccca gcctggaggg ggagtgggca tccgagtggg acagaaggct cagccggtcc
     1081 agcgccgaca cccgccagtt catgggcggg tcagaggaca gtccggtctc cactccgtgg
     1141 atctggtccg ccaggtcccc aaagcactcc tcgacggtgt caaagccgga gaaggcaccg
     1201 aacatggcaa agtggggcgt ccagatatgg gcggggatga attcggcctc tgggcaggtc
     1261 tccagggtca actccagcag atcccggctg tccagcccca gaatggggcg gccgtccgag
     1321 tggatgttgc cgatggcctc cagccggacg gacagttctt ccgccgcttc caggctgggg
     1381 aggaggatca ggttgtgcac cttccgcgtc cgcccgtggc gcttgtagat gcagctgatc
     1441 tccccggtga ccacgaaacg gggggcggcc ccgggcgcag tcccctccat tgtcagcccc
     1501 tcccgcaggg catagacccc ctcccccgcg gggaccagct gctcccgcat ctccgcccgc
     1561 caagccggat gggtgaagtc ccctgtccct atcagctgga tgcctttgcg ccgggcccac
     1621 cagtccaggt ggggcaagtc gcagtcccgg ctggtggccc gggagaacct ggagtggata
     1681 tgaagatcag caatatacat ggatgtcccc ctcttttcac aaggatcccc tccattatac
     1741 cgtttttctc tctccggtgc aatgcacggg ccggctttct cccccttttt ctgtcatatt
     1801 ccgggagttg aagacactcc ctcctctctt ctctccatcg cccgctgcac atcgttggac
     1861 aggatctcca aaaattccga tgcggtgggc ggcccgtcca ggggataccc cgccatctcc
     1921 cgcagcagtt ccgggttggt ccgccacgcg atctgcaccg cagtgcggat gttccgctcc
     1981 accgcggtcc atttccgccc gcagcggctg cccacttccc agtagatctc cttggtcact
     2041 gcctccaggc tgtcctcctg gtccaccgcc agctgaatgg ccattacgat gcggccataa
     2101 cagcggcatc gcctcgtgat ccccagggcg cgcagttccc gtcgaattac atttatttca
     2161 ttcatcgaca tttcctccca atttagtttt aaggaaatca tacgacaaaa tggaagcaat
     2221 tatatcaaat ccgacgatat gcgcttagat tcgtttatat tcgacactcg ctgtccacat
     2281 tccccccttt ttctgcacct cctgccccca cattcctgaa tttttatttc cacctatttt
     2341 gggggctgga aaatcgcttc ctttcatgct atcctctgat agggatcctt ctctgtcccc
     2401 cctgccgcct atcagaaagg agcaatcatg cggacacaac tctggcagct ggcctgcctc
     2461 tattggacca ctgcaaacct gctggccttc gccctgatgg ggctggacaa gcgccgggcc
     2521 cgccggggct gttggcgcat ttcggagaag gccctgttct tctttcccgt cctgggcggt
     2581 tccctggggg ggatgctggg gatgtacttt ttccaccaca agacccgcca ctggtatttt
     2641 cgggcaggct tccctctcct gtttctgttc caggcgctcc tgctcttctt tctgtggcgg
     2701 tggctggcgc cctgatatgc ctggagcagg ctgtccccgc tgtgcgcacg tcttgtccac
     2761 tcacgacagg caaagttcac acttgcagga caagtcgtat tttttcacat acatgccgcc
     2821 gaaaaagcag atttaattca ctctgtcaaa aagtccttca gggacctttt ggcagcctgt
     2881 aattccttcg cacctgcggg tgcgggactc tgcggatcgt ttcctacgag ctgagggggc
     2941 ctgcggtctg cgccgcgccc cccccctccg ctccccgctc tgccggtgtc ggtcccggat
     3001 atgagctgcg cccgcctcaa ctgaggtggg cgcagctccc atttacagct ttttgtcgta
     3061 gcagttcagg atctcccgga taaagccctg gaagaagaac tccgtcccgc ccacaaaggt
     3121 atagcccagc ttgggataca tagtgttggc cggaagattc ccctcatagg tgtccaggcg
     3181 catcaccttt ttccccttcc tgcgggcctc ctcctcgcag aaggccacga actcccgtgc
     3241 cttccctcgc ccagcccacc gggggctgat gcagagggtg tggatcacca tgacatcctc
     3301 cggatccgcc gggacggacc agttcagtgc ggcatattcc gccagctgct ctccgttcag
     3361 gttgacgcag ccgtacacct ctccgtcctc ctccgccaca tacagggtgc cggcctccag
     3421 ggccgcacgc gcgtgttcaa ccgtggggta cttcccccgc tgccagttgg taaaggaggc
     3481 gggccgtttc tcctcctccg ccaggatctc ctcatagatg ctccccacca ggggcaggtc
     3541 ctgctctgtc gccagtcgga tcataccttc tctctccctc tttgtcgatc ttgaaaaaat
     3601 cggggcggct cagcgccgcc ccggtcttct gctttacggc cggtcctgct ccggcttctc
     3661 attgggccct tccccgtcct gagggggcgg tgtctcgtca tcccggggct gggcgggcgg
     3721 ctcctccgga ccgctctgct ccggctctcc gtctgggagc tgcggcgcag acaccggctc
     3781 actgacgatg tggatgttct tggcgggggg ctcgatgtcc ccagtgaggg aacggctcct
     3841 gccgtgggtg gaggcgtaag cgtcctccac cagggcgggg tcccgcccct ggatgatggc
     3901 caccatctcg ccgccggtga tggtctcctt ctccagcagg taggccacca ccttgtgcat
     3961 gtcctccagg ttttcccgga tcagttccac tgcgtcctga tagcactggt ccaggatggc
     4021 ctttaccgcc ttgtccatga cagcggcggt gtcctgggcg cagtccaggc cgtagccccc
     4081 gtccaggtac tggttacgga tggagcccag agccatcata ccaaactcgt cgctcatgcc
     4141 gaacatggct accatgttcc gggccaggtt ggtggcctcc tggatatcct gggaggcacc
     4201 gttggtcatg gtgttgagca ccacctgctc ggcggcccga ccgcccatgg agactgtgat
     4261 tttggccatc agctcttcct tggtgcgcag gttggtcttg tcctcctcgg gcatcagcag
     4321 ggtgtagccc agggaacctt ccgtgtgggg gacgatggtg atcttctgca cgggctcggc
     4381 gttcttctgc ttgtaggcca ccatagcgtg tcccacctcg tggtaggcca ccagcttctt
     4441 ctcaaactcg gtaaggacgc tgttcttctt ctcgcttcca gcgataacga attcgaagga
     4501 ggccagcaga tcctcctggg tgaccactct tcggccgtgc cggactgccc gcagggcggc
     4561 ctcgttcacc aggttggcca agtctgcgcc cacggtcccc gcggtggcca gagcgatctt
     4621 cttgaggtcc acgtcctctc ccagcttgat cttccgggtg tggacctgga gggtggccag
     4681 acggccggcc aggttgggcc ggtcgatggt aatgcggcgg tcgaaccggc cgggccgcag
     4741 cagggccttg tccagcacct cgggccggtt ggtggccccc agcacgatga cgcccttgcc
     4801 cgggtcgaag ccgtccagct cggccagcag ctggttcagg gtctgttccc gctcgtcgtt
     4861 gccgcccatg cggttgtcgc gggacttgcc gatggtgtcg atctcgtcga tgaagatgat
     4921 gcagggggcc atcttgctgg cctccttgaa caggtcgcgg acccggctgg cgcccacgcc
     4981 cacaaacatc tctacaaagt cagagccgga gatggagaag aagggcacgc ccgcctctcc
     5041 agccaccgct ttggccagca gggtcttgcc ggtgcccgga gggcccacca gcagggcgcc
     5101 cttgggcagc ttggcaccga tctccgtgta cttctgggga ttgtggagga tgtcgatgat
     5161 ctcctggagg gattccttgg cctcgtcctg gccagccaca tcccggaagg tgacgccggt
     5221 cttcttctcc acatagacct tggcgttggc cttgcccacg ccgcccaggc cgcccatgcc
     5281 gccctttcca ccgatgcccc ggaacaggaa catcatcagt cccaccatca gcagcagggg
     5341 aagcacatag gagaggatca ggctgaggaa ataggccgac tcgtccaccg gctcggtgta
     5401 ggcgtgatcg gcgtgctcct ccagcatggc cagcagctcc gggtcgtcca ccccggccag
     5461 ggggacggtg atatactgct tttcctcctc cgtctgggcg ggaacaggga tcagggagtt
     5521 catcaggctt ccgctcccgc cggaggtctc ctcccggggc agctccacct ccacgccgtc
     5581 ccgcagggtg aaggtgtatt tgccgttctc cacattgacg gtgtcgatct tgtcctccgc
     5641 cagccactcc ttgaaggtgg tgtagggcac ttccacagta cccatgttgg catagcttga
     5701 ggtgcagctc ttgatcatca ttgtgaacag
//
LOCUS       DBMDSJ010000063         5709 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_3993_length_5709_cov_1.820078, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000063 DBMDSJ010000000
VERSION     DBMDSJ010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5709)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3993_length_5709_cov_1.820078"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(309..2237)
                     /locus_tag="ACZPAQ_02505"
     CDS             complement(309..2237)
                     /locus_tag="ACZPAQ_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR0902882.1"
                     /translation="MRFPTMFSPIQIGGVTVPNRFVVPPMANNFSDPDGCLSQRSLAY
                     YAARARGGFGLITIESAVVYEQAKGGPRKACLFADHTIDSFRRVAEACHSYGAKVSVQ
                     LQHAGPEGSSALTGHPLKAASPVPAACGREIPREMPRDEVYRLIECYGDAARRAQLAG
                     LDMVEVHCAHGYLVSTFLSERTNHRTDEFGGCFENRLRLPRLIVENIRRKTGGIMPIL
                     CRINASDELDGGQSVQDAAAVAAYLEQECGVDAIHVSRAVHLRDEFMWAPGQFHGGFS
                     APLVEEIKRAVTVPVIAVGRFTEPQYAELLVRQGRADLIAFGRQSIADPELPNRARDG
                     QLETLAPCIGCLLGCVPNMLSGRPITCAVNPLVGRELEWGPAGQKKRVAVVGGGPGGL
                     YAAWACARRGHQAVLLEQGEQLGGTLRIASFPTGKGQISAAVRSMIVRCQQAGVEIRT
                     GTRATPELLRQLAPDAAILATGSGPLIPPIPGLDSCGFVTAQDMLEGRAVVGAGVLVV
                     GGGMIGCEAAEFLGERGHRVAVVEMKEDIASDVRPEHRRCLLKNLRESQVLVRTGARV
                     ERFYPDGVDYVLADGTEGSLRGYDSVVLAMGTRSREELRERIAAFVPQVLVVGEAASA
                     PGNAVRATGDALDAALSI"
     gene            complement(2241..2921)
                     /locus_tag="ACZPAQ_02510"
     CDS             complement(2241..2921)
                     /locus_tag="ACZPAQ_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HIR0902883.1"
                     /translation="MEERQTVLRGELALLLAVVINSFGVVLMLRSGSGISAISSVPYA
                     FSELFPRVSMGTWTYIFQGLLVLSLMVLRKRFVPEYLFSFVVGFFFGIMIDFHELWIS
                     WLPQTLPLRVLYFCISYLVICFGIALSNRCRMPIIPTDLFPRELAEITGTAYSRIKIS
                     FDVICLLTTAVLTFFGLGRIMGLGVGTVLAAFTMGKCIGLIGGWMDRRVSFVSILSRR
                     QAAAAEEG"
     gene            3067..3972
                     /locus_tag="ACZPAQ_02515"
     CDS             3067..3972
                     /locus_tag="ACZPAQ_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0902884.1"
                     /translation="MNLSHLRYFVELAHTRHYTRAAEHLFITQPSLSHAIGQLESELG
                     VPLFERSGRNTTLTRLGEEFLVCAERTLSTLDSGVESLRRSAQGEGLIRLGLVRPLGV
                     DFIPDLAARFLAENPGKEIHFTFGTGVTQQLLDGLLARRYDLVFSSMPPAELGLSARP
                     VVRQDLVLVVPPDHPLAGRKSIDLEETLSYPYVSFSRGSGMRDTVDALFGQLQARPRI
                     SCETEEMEVVAGLAARGFGIAVVPRMDLLDQLPLKVISIARPAWERSIYMVSDERIYL
                     SPVVRNFQRFVLEQQGGETETVQKC"
     gene            complement(4030..4761)
                     /locus_tag="ACZPAQ_02520"
     CDS             complement(4030..4761)
                     /locus_tag="ACZPAQ_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR0902885.1"
                     /translation="MDQRTAQRQPAPRLRTNLDTDFLKLIAILSMLLDHIGAVFFPEY
                     PAFRWVGRLAFPIFCYCMTVGLLYTRDIKRYLGRLGLFALISQPCYILATHPWDWQTE
                     WMNWNIFFTLFLSLLAMWGFQTRRWWLFAAAFLCVSLFNFDYSGNGILLMLIFFLCRE
                     RPGLGAGLYILFWLPALWSGSPEDPRALLAAGHAVDWTAFALLAAPFIFLPARTGIRV
                     PKWFFYAFYPAHLLTIGVIRLAAGI"
     gene            complement(4918..>5709)
                     /gene="rny"
                     /locus_tag="ACZPAQ_02525"
     CDS             complement(4918..>5709)
                     /gene="rny"
                     /locus_tag="ACZPAQ_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR0902886.1"
                     /translation="FDPVRREIARLALEKLILDGRIHPARIEEMVEKAKREVDATIKA
                     EGERAVFETNVHGLHPELVKLLGRMHYRTSYGQNVLNHSIEVSHIAGLLAAEIGANVA
                     EAKRAGLLHDLGKSVDHEVEGSHVTIGVELAKKYRESEGVIHAIHAHHNDVEPKTIVA
                     CLVQAADAISAARPGARRENLENYIKRLEMLEEITSSFPGVEKSFAIQAGREVRIMVK
                     PEVVSEDQMVLLARDIAKKIEDELEYPGQIKVHLLRETKAIEYAK"
BASE COUNT         1010 a         1948 c         1803 g          948 t
ORIGIN      
        1 ttcatattgg cggcgggaca ggatatcctg tcccccctcg cccaccacgt ctcccaccgc
       61 caatatgtga agcagcatgt tcgtctcctc cctggagcgc agctccgccg cccggcggcc
      121 cgcctccctc ccctttccgg ggcactgttt ttcctattat acggcatgtt gccccaaagc
      181 gcaaacatct gtttggccgc ggcacctttt ccggcctccc ccctccgagc gccgcctttg
      241 cacacaaagc gcccccgccg gacttttctg cccggcgggg gcgctgtctc ttatttagag
      301 ctgccgtctc agatggacag cgccgcatcc agggcgtctc cggtggcccg gacggcattt
      361 ccaggggcgc tggccgcctc gcccaccacc aggacctggg gcacaaaagc ggcgatacgc
      421 tccctcagct cctcccggct gcgggtcccc atggccagca ccacgctgtc atatccccga
      481 agggagccct ctgtcccgtc ggccaggaca taatccaccc cgtcggggta gaaccgctcc
      541 acccgggcgc cggtgcgcac cagcacctgg gattcccgga ggtttttcaa cagacagcgg
      601 cggtgctccg gccgcacatc ggaggcaatg tcctctttca tctccaccac cgccacccgg
      661 tggccccgct cgcccaggaa ctcggcggcc tcacagccga tcatgccgcc gcccaccacc
      721 aggaccccgg cgcccaccac tgccctgccc tccagcatgt cctgggcggt gacgaagccg
      781 cagctgtcca gtccggggat gggcgggatc agcggcccgg agccggtggc caggatggca
      841 gcgtcggggg ccagctgccg cagcagctcg ggtgtggccc gggtgccggt ccggatctcc
      901 actccggcct gctggcagcg gacgatcatg gaccgcaccg ctgcggagat ctgccccttt
      961 ccggtgggaa aggaggcgat gcgcagggtg ccgcccagct gctccccctg ctccagaagc
     1021 accgcctggt gcccccgccg ggcgcaggcc caggcggcgt acagtccccc gggaccgccg
     1081 cccactacgg ccacccgctt tttctgccct gccgggcccc actccagctc ccggcccacc
     1141 agcgggttga ccgcgcaggt gatgggcctg ccggagagca tgttgggcac gcagcccagc
     1201 agacagccga tgcagggggc cagggtctcc agctgcccgt ccctggcccg gttgggcagc
     1261 tccggatcgg caatgctctg ccgcccgaag gcgatcaggt cggcccgccc ctgccggacc
     1321 agcagctcgg cgtactgggg ctcggtgaag cggcccaccg cgatgacggg caccgtcacc
     1381 gcccgcttga tctcctccac cagcggggcg ctgaagcccc cgtggaactg tcccggcgcc
     1441 cacatgaact cgtcccgcag gtggaccgcc cgggagacat ggatggcatc cacaccgcac
     1501 tcctgctcca gataggcggc caccgcggcg gcgtcctgga cgctctgacc gccgtccagc
     1561 tcgtcgctgg cgttgatgcg gcagaggatg ggcatgatcc cgcccgtctt gcgccggatg
     1621 ttctccacga tcagccgggg caggcggagc cggttctcga agcagccgcc gaactcgtcg
     1681 gtgcggtgat tggtgcgctc agacaggaag gtgctcacca gatagccgtg ggcacagtgg
     1741 acctccacca tgtccagccc cgccagctgg gcccggcggg cggcgtcccc atagcactcg
     1801 atcaggcggt agacctcgtc cctgggcatc tcccggggga tctcccgccc gcaggcggcg
     1861 gggacagggc tggccgcctt cagggggtgg ccggtgaggg cagaactgcc ctccggcccc
     1921 gcgtgctgga gctggacgga taccttagcc ccatagctgt ggcaggcctc cgccacccgg
     1981 cggaagctgt cgatggtgtg gtcggcaaag agacaggcct tccgcggacc gcccttggcc
     2041 tgctcataga ccacagcgga ctcgatggtg atcagcccga agccccccct ggcccgggcg
     2101 gcgtagtaag ccagggaccg ctggctgagg cacccgtccg ggtcggagaa gttattggcc
     2161 atggggggca ccacgaaccg gttgggcacc gtcacgccgc cgatctgaat gggcgaaaac
     2221 atggtgggga atctcatcgt tcagccctcc tctgctgcgg ccgcctgacg gcggctcagg
     2281 atagagacaa agctcaccct gcggtccatc cacccgccga tgagtccgat gcacttgccc
     2341 atggtgaagg ccgccagcac cgtgcccaca cccagcccca tgatccggcc caggccaaaa
     2401 aaggtaagga ccgccgtcgt cagcaggcag atcacgtcaa aggagatctt gatccgggag
     2461 taggccgttc cggtgatctc cgccagctcc cggggaaaca ggtcggtggg gataatgggc
     2521 attctgcagc ggttggacag ggcgatgccg aaacagatga ccaggtagct gatgcagaag
     2581 tacaacaccc gcagaggcag ggtctggggc agccagctga tccagagctc gtggaagtcg
     2641 atcatgatgc cgaagaagaa gcccaccaca aagctgaaca ggtactccgg cacgaaccgc
     2701 ttccgcagga ccatcaggct cagcaccagc agcccctgga agatataggt ccaggtcccc
     2761 atggagaccc ggggaaaaag ctcagaaaat gcatagggca cgctggaaat ggcggagatc
     2821 cctgagcccg agcggagcat cagcaccacg ccaaagctgt tgatcaccac cgccagcaaa
     2881 agggccagct ccccccggag caccgtctgt ctctcttcca tcattgtcat cctcccgcgc
     2941 cggagcgcat gtttttctta cctacaatgt agcgcagggc gggaaaataa acaaatcaat
     3001 tctttttatc aaaaaataga ttttttctat aaaatatggt acactggccc aaaggagggg
     3061 tcctttgtga atctgtcaca tctgcgctac tttgtggagc tggcccacac ccgccactac
     3121 acccgggcgg cggagcacct gttcatcact cagcccagcc tgagccacgc catcggccag
     3181 ctggagtcgg agctgggggt cccccttttc gagcgcagcg gccgcaacac cactctcacc
     3241 cggctggggg aggagtttct ggtgtgcgcg gagcgcaccc tctccacctt ggactccggg
     3301 gtggagtccc tgcggcgcag cgcccagggg gaggggctga tccgcctggg gctggtgcgc
     3361 cccctggggg tggacttcat acccgacttg gccgcccgct tcctggcgga gaatccaggg
     3421 aaagagatcc acttcacctt cggcaccggg gtcactcagc agctgctgga cggccttctg
     3481 gcgcggcgct acgacctggt cttttcctct atgcctccgg cggagctggg gctgagtgcc
     3541 cggccggtgg tccgtcagga cctggtgctg gtggtgcccc cggaccaccc tctggccggg
     3601 cgaaagtcca tcgatctgga ggagaccctg tcctacccct acgtctcctt ctccaggggc
     3661 tccgggatgc gggacacggt ggacgccctg ttcggccagc tccaggcccg cccccgcatc
     3721 tcctgcgaga cggaggagat ggaggtggtg gccggtctgg ccgcccgggg cttcggcatc
     3781 gcggtggtcc cccggatgga cctgctggac cagctgcccc tgaaagtcat ctccatcgcc
     3841 cgccccgcct gggagaggag catctacatg gtcagcgacg agcggatcta cctctctcct
     3901 gtggtccgca acttccagcg gttcgtgctg gagcagcagg gcggggagac ggagaccgtg
     3961 cagaaatgct gatcgacagt ctacacggcc gcccccgcca aaacgaggcc agccgcgctc
     4021 cctttctcct catatcccgg cggccagccg gatcacgccg atggtcagca ggtgagccgg
     4081 gtaaaaggcg taaaagaacc atttggggac cctgatcccc gtccgggcgg ggaggaagat
     4141 gaagggagcc gccaacaggg caaaggcagt ccagtccacc gcgtgccctg ccgccagcag
     4201 ggcacggggg tcctccgggc tcccgctcca cagggcgggc agccagaaca ggatatagag
     4261 ccccgccccc agccccggcc gctcccggca caggaagaag atcagcatca gcaggatgcc
     4321 gttgccggag taatcaaaat tgaagaggct gacgcacagg aatgctgcgg caaagagcca
     4381 ccaccgccgc gtctggaagc cccacatggc cagcagactg aggaacagag tgaagaagat
     4441 gttccagttc atccactccg tctgccagtc ccaggggtgg gtggccagga tgtaacaggg
     4501 ctgggagatg agagcaaaga ggcccagccg gcccagatat cgcttgatat cccgggtgta
     4561 gagcagcccc accgtcatgc agtagcagaa gatggggaag gccagccgcc ccacccagcg
     4621 aaaggcgggg tactctggaa aaaacacggc ccctatgtgg tccagcagca tggacaggat
     4681 ggcgatcagc ttcaaaaaat cggtgtccag gttggtgcgc agccgcgggg ccggctgacg
     4741 ctgcgctgtc ctttggtcca tttctcttcc tcccccccgc tccggcgccc aatgccggag
     4801 cgtcttttgt aggtccagga tagcacaggc cgataaaagg caggtcaaag agagatgaaa
     4861 gttttctgaa aatgggacaa aaatcagggc gcgcttccaa agaagcgcgc cctgctctta
     4921 tttcgcgtac tcgatggctt tggtctcccg cagcagatgc accttgatct ggccggggta
     4981 ctccagctcg tcctcgatct tcttggcaat gtcccgggcc agcagcacca tctggtcctc
     5041 gctgaccacc tcgggcttga ccatgatgcg gacctcccgg cccgcctgga tggcgaagga
     5101 cttctccacc ccggggaagg aggaggtgat ctcttccagc atctccagac gcttgatgta
     5161 gttctccaga ttctcccgcc gggctccggg ccgggccgcg gagatggcgt cggccgcctg
     5221 gaccaggcag gcgacgatgg tcttgggctc cacatcattg tggtgggcgt ggatggcgtg
     5281 gatgacgccc tcgctctccc ggtacttctt ggccagctcc acgccgatgg tgacatggga
     5341 gccctccacc tcgtggtcga cggacttgcc caggtcgtgg agcagtccgg cccgcttggc
     5401 ctcggccacg ttggcgccga tctcggccgc cagcaggccg gcgatgtggg agacctcgat
     5461 ggagtggttg agcacgttct gtccgtagct ggtgcggtag tgcatccggc ccagcagctt
     5521 gaccagctcg gggtgcagac cgtggacatt ggtctcaaag accgcgcgct ccccttcggc
     5581 cttgatggtg gcgtccacct cccgcttggc cttctccacc atctcctcga tgcgggcggg
     5641 gtggatgcgg ccgtccagga tcagcttctc cagggccagc cgggcaatct cccggcggac
     5701 ggggtcaaa
//
LOCUS       DBMDSJ010000064         5660 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4039_length_5660_cov_1.678000, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000064 DBMDSJ010000000
VERSION     DBMDSJ010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4039_length_5660_cov_1.678000"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="ACZPAQ_02530"
     CDS             <1..478
                     /locus_tag="ACZPAQ_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902887.1"
                     /translation="VFCALLLAGCSISSQSRQGQPLAVYSFSGENEFISVSNGVIVLD
                     AEEEICYGGNLETRKDKFTDIASYSATIYFTSDGEENILMSNRVEDKTGGAINISEDI
                     GKITGDILIPDDIDNLVDGLWFELKTVDLDGEENTYHLQLEVIEITRANDNSPSAY"
     gene            729..1268
                     /locus_tag="ACZPAQ_02535"
     CDS             729..1268
                     /locus_tag="ACZPAQ_02535"
                     /inference="COORDINATES: protein motif:HMM:NF017856.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIR0902888.1"
                     /translation="MKQFKFPHIGMRTTKTAVAVMLSYLIFVPFGLLYNESYPGVLAY
                     VGPTYSCIACIVCMQSSLEQTLQVGLSRFVGVFIGAILGVAMLLLEPLLSHWVGIVLM
                     LGAACVCGIWLCMLFNRPAACGMACILPCVMLISGDVSGIQRYYYAAARVSETVVGVT
                     VAFLINSLLPTRNPQPQDK"
     gene            complement(1356..2741)
                     /gene="purF"
                     /locus_tag="ACZPAQ_02540"
     CDS             complement(1356..2741)
                     /gene="purF"
                     /locus_tag="ACZPAQ_02540"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR0902889.1"
                     /translation="MEDIRLEQGLHEECGVFGIYDPEGSCAQTTYYGLYALQHRGQEA
                     CGIAAINDREQSFYKDVGLVSEVFDRETLQRLNGTMVVGHVRYATTGAGARENAQPLT
                     IKYVKGTLAVVHNGNLVDVDRLRARFEYQGAIFHTTSDSELIAYAIAQARLHGTSVED
                     AVCRAVGELRGAFSLIVMSPRKLIACRDPWGFRPLCMGRKGKAVLFASESCAIESVGG
                     TLERELDPGEIVVVEEGQVRSIRDNCRGQSHMCIFEYIYFARPDSVIAGQSVHEARVN
                     AGRLLARRHPVEADLVVGVPDSGLDAARGYAQESGIPYGVGFVKNRYVGRTFITPDQT
                     SREQAVRIKLSALRSEVEGRRVVMIDDSIVRGTTCRQITNLLREAGAKEVHIRVSAPP
                     FIAPCYFGTDIPDRERLIACQHSLEEIREIAGADSLGYLPLEDLHQLAPKAACGFCDG
                     CFTERYPVDLE"
     gene            2929..>3232
                     /locus_tag="ACZPAQ_02545"
     CDS             2929..>3232
                     /locus_tag="ACZPAQ_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902890.1"
                     /translation="MDHDQQFLSFIHKNAAMGVSAIPQVLSLPQSRAMGQALNSQLQE
                     YRTIAAQSQAYARKHGARLHTPGLAVRAMSGAMLKARTAADHTTSKLAQLMIQGSTM"
     assembly_gap    3233..3242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3661..4116)
                     /gene="nrdR"
                     /locus_tag="ACZPAQ_02550"
     CDS             complement(3661..4116)
                     /gene="nrdR"
                     /locus_tag="ACZPAQ_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR0902891.1"
                     /translation="MKCPFCAYLESKVVDSRPADEGASIRRRRECLSCHKRFTTYETM
                     ESLPLMVVKKDGSRQSFDRNKVMNGLIRACEKRPVSFSTMEEIVNEIEQTLQNEMERE
                     VSSAEIGELVMERLKKVDEVSYVRFASVYRQFKDINTFMRELNKLLEDK"
     gene            complement(4267..>5660)
                     /gene="gltX"
                     /locus_tag="ACZPAQ_02555"
     CDS             complement(4267..>5660)
                     /gene="gltX"
                     /locus_tag="ACZPAQ_02555"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR0902892.1"
                     /translation="HVGNLRTALYTWLIARHHQGTFILRIEDTDQTRQVEGATEVIYR
                     TLAECGLDHDEGPDVGGPVAPYIQTQRRDTYGKYARLLVEKGHAYYCFCEKTESEEDS
                     GQFDRASDPCRALSQEEAQARVDAGEPYVIRQKIPSGGTTTFHDAIFGDITVENDTLD
                     DQVLIKRDGLPTYNFANVIDDHLMGITHVVRGSEYLSSAPKYDLLYQAFGWEVPTYVH
                     CSPVMRDAHNKMSKRHGDPSYEDLKAQGYLTPAILNYVALLGWSPRGELAEQEVFSLE
                     ELVAAFDLAGVSKSPAIFDIEKLTHFNALYLRAMEPADFARAAEPYIRQSVKDPALDA
                     AEIAGLLQARCERLTDIPEKVDFFDALPDYDGALFTNKKSKTDPEVARAMLEAAIPAL
                     EGLDGWTTEGIHDALIALAEKLAVKNATLMWPVRIAAAGKAVTPGGAVEICHVLGREE
                     TLRRLRLGLDKLR"
BASE COUNT         1055 a         1771 c         1665 g         1159 t
ORIGIN      
        1 cgtattctgc gctttgctgc tcgcagggtg cagtatttct tctcaaagca ggcaagggca
       61 gccgctagca gtctattctt ttagcgggga aaatgaattt atttctgttt caaatggggt
      121 catcgtttta gacgctgaag aagagatctg ttatggcggt aatttggaga caagaaaaga
      181 taagtttacg gatattgctt catactcagc taccatttat tttacatcag atggtgaaga
      241 gaatatttta atgtccaata gagttgaaga taaaacagga ggcgcaataa atatttccga
      301 ggatatagga aaaataaccg gggacattct tatccctgat gatattgata atttagttga
      361 tggtctttgg tttgagctga aaacagttga tttggatgga gaggaaaaca catatcatct
      421 gcaattagaa gtgattgaga tcactagagc gaatgataat tcgccttctg cttattgacc
      481 cgtgtgacct ggagcagatc cccctcatct gaatggacgc ggggagggtt tctgtcctga
      541 agaccggtcc agaggggaaa agggtacagg gcgtggacgg cttgactttc cccccgcctc
      601 aggatacaat cgagttgttt tccaaactct cccctctccc ctgttactgc tcagactttc
      661 cgggcatcct agcagggcgc cccggctgcg gggcctggca caacaggaca cagcaggagg
      721 tcttccccat gaaacagttc aagttcccac atatcgggat gcgcaccact aagaccgccg
      781 tggcagtgat gctctcctac ctgatctttg tccctttcgg cctcctctat aacgagagct
      841 accctggggt gctggcctat gtgggcccca cctattcctg catcgcctgc atcgtgtgca
      901 tgcagagctc cctggagcag accctccagg ttgggctctc ccgctttgtg ggggtgttca
      961 tcggtgccat cctgggggtg gctatgctac tcctggagcc cctgctgtcc cactgggtgg
     1021 gcatcgtcct gatgctggga gccgcctgtg tgtgcgggat ctggctttgc atgctcttca
     1081 accggcccgc ggcctgcggc atggcctgca tcctcccctg cgtcatgctc atttccggtg
     1141 acgtgtccgg catccagcgc tactactacg ccgccgcccg ggtgtcggag acggtggtgg
     1201 gagtaacggt ggccttcctc atcaactccc tgctccccac cagaaatcca cagccccagg
     1261 ataagtgaca ggctgaactc cttgtcagca agaacccacc aaaaaagaac aggcccgcgc
     1321 gtccgcagaa cactgcaggc gcgcgggctc gtttttcact ccaggtccac cggatagcgc
     1381 tcagtgaagc agccgtcaca gaagccgcag gccgccttgg gggccagctg gtgcaggtcc
     1441 tccagaggca gatagcccaa gctgtccgcc ccggcgatct cccggatctc ctctagggaa
     1501 tgctggcagg cgatgagccg ttcccggtcc gggatatcgg ttccaaagta gcatggggcg
     1561 atgaaggggg gcgcggacac ccggatgtgg acctccttcg ccccggcctc ccgcagcagg
     1621 ttggtgatct ggcggcaggt ggttccccgg acgatggagt cgtcgatcat caccacccgc
     1681 ctgccctcca cctcactgcg caaggcagac agcttgatgc gcaccgcctg ctcccggctg
     1741 gtctggtctg gagtgatgaa agtgcggccc acgtagcggt ttttcacaaa gcccaccccg
     1801 taggggatgc cgctctcctg ggcatagccc cgggcggcgt ccagcccgga gtcaggcacc
     1861 cccaccacca ggtcggcctc cactggatgg cggcgggcca gcagacggcc ggcgttcacc
     1921 cgggcctcgt ggactgactg cccggcgatc accgagtcgg gccgggcgaa gtagatatat
     1981 tcgaagatgc acatgtggga ctggcccctg cagttgtctc ggatggagcg cacctgcccc
     2041 tcctccacca ccacgatctc tcctgggtcc agttcccgct ccagggtgcc ccccacgctc
     2101 tcgatggcgc aggactcaga ggcgaacagc accgccttcc ccttccggcc catgcacagg
     2161 gggcggaagc cccaggggtc ccggcaggcg atgagcttac ggggggacat gacgatcagg
     2221 gagaacgccc ctcgcagctc ccccacagcc cggcacaccg cgtcctccac gctggtcccg
     2281 tggagccggg cctgggcaat ggcgtaggca atgagctccg agtcgctggt ggtgtggaag
     2341 attgccccct ggtactcaaa ccgggcccgc agccggtcca cgtccaccag attgccgttg
     2401 tggaccaccg ccagcgttcc cttcacatac ttgatggtca ggggctgggc gttctcccgc
     2461 gccccagcgc cggtggtggc gtagcgcaca tggcccacca ccatggtccc attcaggcgc
     2521 tgaagggtct cccggtcgaa gacctcgctg accagtccca cgtccttata aaaggactgc
     2581 tcccggtcat tgatggccgc gatgccgcag gcctcctgcc cccggtgttg gagggcgtac
     2641 agcccgtaat aggtggtctg ggcgcagctg ccctccgggt catagatccc gaatacaccg
     2701 cactcctcgt ggagcccctg ctccagccgg atgtcttcca tactcacgat attctccgcc
     2761 cctttcgact ccggcgcccc gcgtgccccg ggaaaaagat agtttattat agcgcccccc
     2821 gctccgacca tcaaggacag gatctcaaat atttttacaa aatccctgct ctttctcgta
     2881 tattttgcag aatttttcag ataatatgga ggaagggaga gaaatttcat ggatcacgat
     2941 cagcagtttt tgtctttcat tcataaaaac gcggctatgg gggtgagcgc catcccccag
     3001 gtgctgtccc tgccccagag ccgggccatg ggccaggccc tgaacagcca gctccaggaa
     3061 taccggacca tcgccgccca gtcacaggcc tatgcccgga agcatggtgc ccggctccat
     3121 accccgggtc tggctgtccg ggccatgtcc ggtgccatgc tcaaggcccg cactgccgcc
     3181 gaccacacca cctccaagct ggctcagctg atgatccagg ggagcaccat ggnnnnnnnn
     3241 nngacccggc gcatccacca gtacaccggc aaggcggacc cggagctgct ggccctgggc
     3301 agccgcctgc tgcacaccga ggaacagaac atccagcaga tgaagcggtt cctttaagct
     3361 ccgccctggc gcggcacaaa aagcgggagg ccgatggctc ggccccccgc ttccgctagt
     3421 gaaaaatctc ccccagaggg ggccccaaag agttctgtcg tccgtggaag acaggatcaa
     3481 atcaaaaagc attatttccc gggacatgct tctggaaaat gacacctctc atgacagatg
     3541 aggcggcttt ttcctgcaaa aataggcctc atctgtcatg cagcccttcg tcgcaaaaga
     3601 cccgacggcc ttttgcgaca atttgagctc cgggaccatg tggtcccggg gccggattgc
     3661 tcacttatct tccagcagct tgttcagctc ccgcatgaag gtgttgatgt ccttgaactg
     3721 gcggtagacg gaggcaaagc gcacatagga gacctcgtcc acttttttca gccgctccat
     3781 caccagctct ccgatctcgg cggagctgac ctcccgctcc atctcgttct ggagggtctg
     3841 ctcgatctcg ttgacgatct cctccatggt gctgaaggag accggccgct tttcacaggc
     3901 ccggatcagg ccgttcatca ccttgttccg gtcaaagctc tggcggctgc cgtccttttt
     3961 gaccaccatc aggggcaggc tctccatggt ctcgtaggtg gtgaagcgct tatggcagga
     4021 cagacactcc cggcggcggc ggatgctggc tccctcgtcc gcgggacggg agtccaccac
     4081 cttgctctcc agataggcgc agaaggggca tttcatctcg attcctccat tctgtggttg
     4141 cccggctgcc gcgggcatgt gttttctccg ctattatacc atcccggccc agccttgaac
     4201 agcctgtttt ttcctgtttc ggaaaaaatg tccccggcgg cacaggccgc cggggacagg
     4261 tggatcttat cgcagcttgt ccagccccag gcgcaggcgg cgcagagtct cctcccggcc
     4321 cagcacgtgg cagatctcta ccgcgccccc gggagtgacc gcctttccgg cggcggcgat
     4381 gcgcaccggc cacatgaggg tggcgttctt caccgccagc ttctccgcca gagcgatgag
     4441 ggcgtcgtgg atgccctccg tggtccagcc gtccagcccc tccagggcgg ggatggcggc
     4501 ctccagcatg gcccgggcca cttccgggtc ggtcttggac ttcttgttgg tgaacagagc
     4561 cccatcatag tcaggcaggg catcgaagaa gtccaccttc tccgggatgt cagtgagccg
     4621 ctcacaccgg gcctggagca ggccggcaat ctccgccgcg tccagcgccg ggtctttcac
     4681 gctctggcgg atatagggct ctgccgcccg ggcaaagtcc gccggctcca tggcccgcag
     4741 gtacagggcg ttgaagtggg tgagcttctc aatatcaaag atggccgggg acttggatac
     4801 acccgccaga tcaaaggcgg ccaccagctc ctccagagag aatacttcct gttccgccag
     4861 ttctcctctg ggggaccagc ccagcagggc cacatagttc aggatggcgg gggtgaggta
     4921 gccctgggct ttcaggtcct cgtaggaggg gtccccgtgg cgcttggaca tcttgttgtg
     4981 ggcatcccgc atcacggggg aacagtgcac ataggtgggc acttcccagc caaaggcctg
     5041 atagagcaga tcatacttgg gagcagagga cagatactcg ctgccccgga ccacatgggt
     5101 gatgcccatc aggtggtcgt cgatgacatt ggcgaagtta taggtgggca ggccgtcccg
     5161 cttgatgagc acctggtcgt ccagggtgtc gttctccacg gtgatgtccc cgaagatggc
     5221 gtcatggaag gtagtggtcc cgccgctggg gatcttctgg cggatgacgt atggctcgcc
     5281 cgcgtccacc cgggcctgag cctcctcctg gctcagggcg cggcaggggt ccgaagcccg
     5341 gtcgaactgg ccggagtcct cttccgactc ggtcttttca cagaagcagt agtaggcgtg
     5401 tcccttttcc accagcagcc gggcgtactt tccataggtg tcccggcgct gggtctggat
     5461 atagggggcc accgggccgc ccacgtcggg gccctcgtcg tggtccagcc cgcactccgc
     5521 cagggtgcgg tagatcacct ctgtggcgcc ctccacctgc cgagtctggt cagtgtcctc
     5581 gatgcgcagg ataaaggtgc cctgatggtg ccgggcgatg agccaggtgt aaagggcggt
     5641 gcgcagattg cccacgtgca
//
LOCUS       DBMDSJ010000065         5649 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4047_length_5649_cov_1.671774, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000065 DBMDSJ010000000
VERSION     DBMDSJ010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4047_length_5649_cov_1.671774"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="ACZPAQ_02560"
     CDS             complement(<1..1488)
                     /locus_tag="ACZPAQ_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HIR0902893.1"
                     /translation="MLQKIKSLSLERQLFLSFSAVSALLLMLSLGITLYFDIARQSQS
                     IDSTISSTAAYVASMDGVAEMLANGYPTQEVTGSLDSLHQNFPGIDIIAVYDRNGLRF
                     YHTSRLETGETFVAGEETAILSGSPPYITTGYGTHGNQRVAFHAVRDGSGSIIGFVTA
                     AIFRADILQRSLNLVLLFSLVLGGMFGVALLLARGIVHLLKGSLRGHHPKELLDLYLQ
                     QDDLLNSIEDGLVATDLRGQVVFANDQARTLLQCGSTPLEGQPLTRFFPETGCIQVAR
                     SGQAVHNRSCVIQERQILASEVPILGENGSQGVLNVFHDKTEMRKLSDELSGARYMLD
                     TLRFFNHEFMNKLHIILGYLQTGESQKAVQFIMNTSLVTSQSIRETADCIRVSHLCAL
                     IIGKMMHAAELGILLTVTHDSFCREEDLLLSPEDYATILGNLLENAIDELSRSHSEVR
                     EIKLSMYCRPDCNLVVCEDTGGGIAPDILPHIWDKGFSSKGEGRGF"
     gene            complement(1522..2712)
                     /locus_tag="ACZPAQ_02565"
     CDS             complement(1522..2712)
                     /locus_tag="ACZPAQ_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR0902894.1"
                     /translation="MTTLFSPIREFFKKGDVLLLFLCLTASGYGLALIYSATRYLDNN
                     NRNVIVQAVAICIGVVIYIVLTFVDFQLFVEKCWKWLVAFDVGFILLLLTPLGKESGG
                     NRNWLALDRIIPHFPLDLQPNEIVKIPFILLLAWQAARIHQQERDISSPFSVAQLTGH
                     TLLMVGLIAVVCGDIGMCVIYLFLFVTISWMAGVKARWFALGGTCFVGAILGAWFTIL
                     QSDRFAYIRNRFMVVLDHSLDPLGAGFQQSRSLLAIGSGQLTGQGYLNGTQTQASYSA
                     ALPARQTDFIFSVCGEELGMVGCLLLLLVLALIILRCYWVGRNASSPFSAYVSMGIAG
                     MLLAQVFFNVGMCLYILPVMGLTLPFFSYGGSSIITLYTAMGIVSSIRARTLPSWLRD
                     RNQL"
     gene            2959..3390
                     /gene="tsaE"
                     /locus_tag="ACZPAQ_02570"
     CDS             2959..3390
                     /gene="tsaE"
                     /locus_tag="ACZPAQ_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR0902895.1"
                     /translation="MEFVTNSPEETEELGERLGRALSPGDVVAFTGDLGAGKTAFTRG
                     IARGLGIGDRVTSPTFAIVNEYEGGRLPLFHFDMYRLTSSDELFDIGWEDYLARGGVC
                     AVEWSENVADALERDTVRVDIRRGGEESRRVISVEPVPAGF"
     gene            3411..4121
                     /gene="tsaB"
                     /locus_tag="ACZPAQ_02575"
     CDS             3411..4121
                     /gene="tsaB"
                     /locus_tag="ACZPAQ_02575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR0902896.1"
                     /translation="MKILALETSAAACSAALCQDGTLLAQSWQNSGLTHSRTLLPLVD
                     SMLKNCGASLKEVEVIAVAAGPGSFTGLRIGVATAKGLAWPEDKPCAPCSTLESMAWP
                     LAHLEGRVIVCAMDARRSQVYNALFLAAGEGLQRLTPDRAIGLDQLGEELKKVEKEKI
                     VVGDGARLCYNTLTEQGITLKMAPEHLRMQSAWGVARAALELVRSGGLVSGEELAPVY
                     CRLSQAERERLEREAKSK"
     gene            4139..4774
                     /gene="upp"
                     /locus_tag="ACZPAQ_02580"
     CDS             4139..4774
                     /gene="upp"
                     /locus_tag="ACZPAQ_02580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR0902897.1"
                     /translation="MFDSKVHVLDHPLLQHKLSILRDERTGVKEFREIVSEIATLECY
                     EATRDLPLEDVEIKTPVATGTFKCLTGKKLAIVPILRAGLGMVDGILTLIPSAKVGHI
                     GLYRDPETLAPVEYYCKMPHDIAERDVIVLDPMLATGGSAVAAIQFLKNYECRSIKLM
                     NILAAPEGIQAVCTAHPDVDIYVAAVDEKLNDHGYIVPGLGDAGDRIFGTK"
     gene            4978..5220
                     /locus_tag="ACZPAQ_02585"
     CDS             4978..5220
                     /locus_tag="ACZPAQ_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR0902898.1"
                     /translation="MDIHVGDVLKMKKAHPCGSQEWLVLRVGMDFRMRCLGCGHEVML
                     PRNKAEKSVRKVYRDGTEVKLEARPAKGGQGGGEEA"
     gene            5217..>5649
                     /locus_tag="ACZPAQ_02590"
     CDS             5217..>5649
                     /locus_tag="ACZPAQ_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0902899.1"
                     /translation="MNQRYWSSRIQGLVPYTPGEQPKDRTFIKLNTNENPYPPSPRVL
                     EAIRREADQRLRLYPDPESAALRGALAEHYGVEPEQVFCGNGSDEVLGLCFYALFSPG
                     RKVVFPDITYSFYPVYTELFGLDYEEIPLNEDFTLPVERFLG"
BASE COUNT         1160 a         1562 c         1967 g          960 t
ORIGIN      
        1 gaagccacgc ccctccccct tagaggagaa tcctttgtcc cagatgtggg gcaggatgtc
       61 gggggcgatg ccgccgccgg tgtcctcgca gaccaccaga ttgcagtcag ggcggcagta
      121 catggacagc ttgatctccc gcacctcgct gtggcttcgg ctgagctcgt cgatggcgtt
      181 ttccaacagg ttgcccagga tggtggcata gtcctccggg gagaggagca ggtcctcctc
      241 ccggcagaag ctgtcgtggg ttacggtgag caggatgccc agctcggcgg cgtgcatcat
      301 cttcccgatg atgagggcgc acaggtggga cacccggata cagtccgccg tctcccggat
      361 ggactggctg gtgaccagac tggtgttcat gatgaactgg acagctttct ggctctcgcc
      421 ggtctggaga tagcccagga tgatgtggag cttgttcatg aactcgtggt tgaaaaagcg
      481 cagggtgtcc agcatgtagc gggcaccgga gagctcgtcg gacagcttgc gcatctccgt
      541 cttgtcgtgg aagacgttga gcaccccctg ggagccgttc tcccccagga tgggcacctc
      601 actggccagg atctggcgct cctggatgac gcaggagcgg ttgtgcaccg cctgcccgct
      661 gcgggccacc tggatgcagc ccgtctccgg gaagaagcgg gtcagcggct gtccctccag
      721 gggggtggag ccgcactgga gcagggtccg ggcctggtcg ttggcaaaga ccacctgccc
      781 ccgcaggtcg gtggccacca ggccgtcctc gatggagttg agcaggtcgt cctgctgtaa
      841 gtagaggtcc agcagttcct tggggtggtg cccgcggagg gagcccttca gcagatggac
      901 gattccacgg gcgagcagaa gggccacacc gaacatgccg cccagcacca gagagaagag
      961 cagcacgaga ttgaggctgc gctggagaat gtcggcccgg aagatggccg cggtgacaaa
     1021 gccgatgatg gagccagacc cgtcccggac cgcatggaaa gccacccgct ggttgccgtg
     1081 ggtgccgtag ccggtggtga tatagggcgg ggagccggac aggatggcag tctcctcccc
     1141 ggctacaaag gtctcgccgg tctccaggcg gctggtgtgg taaaagcgca gaccgtttcg
     1201 gtcatagaca gcaatgatgt caatgccagg aaaattctgg tgcagggaat ccagcgaccc
     1261 ggtgacctcc tgggtgggat agccattcgc cagcatctcc gccacgccat ccatagaggc
     1321 cacataggcg gcggtgctgg agatggtgga gtcgatgctc tgactttggc gggcgatatc
     1381 gaaatagagg gtgatcccca gagagagcat caggagcagg gcggagacag cagaaaagct
     1441 gaggaagagc tgccgctcca gggagaggct tttgatcttt tgcagcatgg agtcacctcc
     1501 gcgatcgagg gtcggccagg gtcagagctg gttccggtcc cgaagccaac tggggagggt
     1561 cctggcccgg atgctggaga cgatgcccat ggcggtgtaa agggtgatga tggaggagcc
     1621 gccatagctg aaaaagggga gcgtgagccc catgacaggc aggatgtaca ggcacatgcc
     1681 cacattgaag aatacctggg ccagcagcat cccggcgatc cccatggata cataggcgga
     1741 gaagggggag ctggcgttcc ggcccaccca gtaacaccgc aggatgatga gggccaggac
     1801 caggagcagc aggaggcagc ccaccatgcc aagctcctcc ccgcagacgg agaagatgaa
     1861 gtcggtctgt cgggcaggga gagcggcact gtatgatgcc tgagtctggg tgccgttgag
     1921 gtatccctgg ccggtgagct ggcccgagcc gatggccagc agggaacggc tctgctggaa
     1981 gccagccccc agcggatcca ggctgtggtc caatacgacc ataaaccgat tgcggatata
     2041 ggcgaagcgg tcactctgga ggatggtgaa ccaggcccca agaatggcac ccacgaagca
     2101 cgtcccgccc agagcgaacc atcgagcctt cacccccgcc atccaggaga tggtgacgaa
     2161 gaggaacaga tagatgacgc acataccgat gtcaccgcag accacggcga tcagccccac
     2221 catcagcagg gtatgaccgg tgagctgggc cacggagaag ggggagctga tgtcccgctc
     2281 ctgctggtgg atgcgggcgg cctgccaggc cagaagcagg ataaagggga tcttgacgat
     2341 ctcgttgggc tggagatcca aggggaagtg ggggatgatc cggtccaggg cgagccagtt
     2401 ccggttgccc cccgattcct ttcccagggg ggtcaggagc aggagaatga agcccacatc
     2461 aaaggccacc agccacttcc agcacttctc cacgaacagc tggaagtcca caaaggtgag
     2521 gacgatatag atgaccacgc cgatgcagat ggcgacggcc tggacgatca cgttccgatt
     2581 attattatcg agatagcggg tggcgctgta gatcagggcc aggccgtagc cgctggccgt
     2641 gagacatagg aacagaagga gcacatcgcc cttcttgaaa aactcccgga tgggggaaaa
     2701 cagggtggtc acgttgtcac ctcttcagta gggggaaata ttttctttca aattgttatt
     2761 ttaccgcctt tgtgtgaaaa agtaaactga cagttctgaa aaaagggcgg ttgggaccgc
     2821 gtttcctccc ggcatgtgga gctgcgggcg ggaaaagcat gaggacggcg gaaacatcac
     2881 tggaaaagcg tgggggacct gtgttataat cggaggcgag aacgtggagc cgcgatgtcc
     2941 gcggggagag gagcgagcat ggaatttgtg accaacagcc ccgaggagac ggaggagctg
     3001 ggagaacggc tggggcgtgc cctgtccccg ggggatgtag tggcctttac cggggacctg
     3061 ggggcgggca agaccgcatt cacccggggc atcgcccggg ggctggggat cggggatcgg
     3121 gtgaccagtc ccacctttgc cattgtcaac gagtatgaag gggggcggct gccgttgttc
     3181 cacttcgaca tgtaccgcct gacctcctcc gacgagctgt tcgacatcgg ctgggaggac
     3241 tacctggccc ggggcggcgt gtgcgccgtg gagtggagcg agaatgtggc ggacgcgctg
     3301 gagcgggaca ctgtccgggt ggacatccgc cggggcgggg aggagagcag gcgggtgatc
     3361 tcggtggagc ctgtccccgc cggattttga gcagagaggg gagatcgacg atgaaaatac
     3421 tggcccttga gacctcagcg gcggcctgtt ccgccgccct gtgccaggac gggaccctgc
     3481 tggcccagag ctggcagaac agcgggctga cccacagccg gaccctgctg cccctggtgg
     3541 acagcatgct gaagaactgc ggcgcgtccc tgaaggaggt ggaggtaatc gccgtggcgg
     3601 ccggccccgg ctccttcacc gggctgcgca tcggcgtggc taccgcgaag gggctggcct
     3661 ggccggagga caagccctgc gccccctgct ccaccctgga gtccatggcc tggcctttgg
     3721 cccacctgga ggggcgggtc atcgtgtgcg ccatggatgc ccggcgcagc caggtgtaca
     3781 acgccctctt cctggccgca ggggagggcc tccagcggct gacgcccgac cgggccatcg
     3841 gcctggacca gctgggggaa gagctgaaaa aggtggaaaa agagaaaatt gtagttggag
     3901 acggggccag gctgtgctat aatactttaa cagagcaggg tatcaccctg aaaatggccc
     3961 cggagcatct gcggatgcag agcgcctggg gtgtggcccg cgcggcgctg gagctggtcc
     4021 gttcaggcgg actggtctcc ggagaggagc tggcccccgt gtactgccgc ctgtctcagg
     4081 cggagcggga gcggctggag cgggaagcta aatcaaaata aaaaggggag cgtttcagat
     4141 gtttgacagc aaagtccatg ttttggatca cccgctgctc cagcacaagc tgagtatcct
     4201 gcgggacgag cgcaccggtg tgaaggagtt ccgggagatc gtgtccgaga tcgctacact
     4261 ggagtgttat gaggctaccc gtgatctgcc tctggaggat gtggagatca agactcccgt
     4321 ggccaccggc accttcaagt gcctgacggg gaagaagctg gccattgttc ccatcctgcg
     4381 ggccgggctg ggcatggtgg acggcatcct gacgctgatc ccctccgcca aagtgggaca
     4441 catcggcctg taccgggacc cggagaccct ggcaccggtg gagtattact gcaagatgcc
     4501 tcacgacatc gccgagcggg acgtcatcgt cctggatccc atgctggcta ccggcggctc
     4561 cgccgtggcg gccatccagt tcctgaaaaa ctatgagtgc aggagcatca agctgatgaa
     4621 catcctggcc gcccctgagg gcatccaggc agtgtgtacc gcccatcccg atgtggacat
     4681 ctatgtggcc gcggtggacg agaagctcaa tgaccacggc tatatcgtcc ccggcctggg
     4741 cgacgcgggc gaccgaattt tcggcactaa ataagtatcg gatacaggca aggcggcggg
     4801 cccctctggc aggaagggtc cgccgcctct gcactgttgt cattttctgg ggtgctgggg
     4861 atgaaaggga gacggcgagg gaggatttga caggggcgcg gaattcctct ataatagtaa
     4921 aaaactgcaa aaggaagcgg cccgagccag agagaggccg gaatggagga gcatgtgatg
     4981 gacatccatg tgggagatgt gctgaaaatg aagaaggctc acccctgtgg aagccaggag
     5041 tggctggtcc tccgggtggg catggatttt cggatgcgct gtctgggctg cggacacgag
     5101 gtgatgcttc cccggaacaa ggcggagaaa agtgtccgaa aggtgtatcg ggacggcacg
     5161 gaggtcaaac tggaggcgcg cccggcaaag ggcggccaag gaggagggga agaggcatga
     5221 atcaacgata ctggagcagc cggatccagg gactggtgcc ctacaccccc ggggagcagc
     5281 ccaaggaccg gacgttcatc aagctgaaca ccaacgagaa cccctacccc ccttctcccc
     5341 gggtgctgga ggccatccgg cgggaggcgg atcagcgcct gcggctctat cctgatccgg
     5401 agtctgccgc gctgcggggg gcgctggcgg agcactatgg cgtggagccg gagcaggtgt
     5461 tctgcggcaa cggctcagac gaggtgctgg ggctgtgctt ttacgccctg ttcagccccg
     5521 ggcggaaggt ggtgttccca gacatcacat acagcttcta tccggtctat accgagctgt
     5581 tcgggctgga ctatgaggag atccccctca acgaggactt caccctgccg gtggagcgct
     5641 ttctggggg
//
LOCUS       DBMDSJ010000066         5426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4238_length_5426_cov_1.807324, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000066 DBMDSJ010000000
VERSION     DBMDSJ010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_5426_cov_1.807324"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="ACZPAQ_02595"
     CDS             complement(<1..543)
                     /locus_tag="ACZPAQ_02595"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR0902900.1"
                     /translation="MNDFQYYTPTKVVFGRGTEEQAGQLVKEQGGTRALVHFGGRSAR
                     ESGLLDRVLASLDRAGVAHVELGGVVPNPRLSKVREGMELCRREGVDFLLAVGGGSVI
                     DSAKAIALGLAYEGDVWDLFSGQPAQDCLPVGTVLTIAAAGSEMSNSCVITNEDGWKK
                     RGLNTDLCRPRFAVMDPELTF"
     gene            complement(631..1875)
                     /locus_tag="ACZPAQ_02600"
     CDS             complement(631..1875)
                     /locus_tag="ACZPAQ_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR0902901.1"
                     /translation="MSIVKDLSLSPSGLQKIQWVRDFMPALGGIEERFRREKPFEGLT
                     IAVSVHLEAKTANLGLVLREGGAQVYLTGCNPLSTQDDVAAAMASLGVETFGVHGVDM
                     RGYEDLLTETLKCRPHLIVDDGGDLISLLGGRCAPYGDRLLGGCEETTTGIHRLYARE
                     RAGILPCPMMAVNDAKAKHYYDNKYGTGQSTWDAIMHTTNLMVAGKTVVVAGYGWCGK
                     GIAMRAKGMGANVIVTEVDPFKALEATMENFRVMPMDEAARYGDIFVTATGCKDVIVE
                     RHFQVMKHNVLLCNSGHFDCEVDVARLGQLAEERFPRRAGIEGFRLPDGRVLNVLAEG
                     RLVNLAAGNGHPAEIMDMSFAVQALSLEWMARHAGKLEKKVYNVPEEIDDQIGRVKLA
                     AMGLSIDTLTQEQKDYLAGWEA"
     gene            complement(1910..2881)
                     /locus_tag="ACZPAQ_02605"
     CDS             complement(1910..2881)
                     /locus_tag="ACZPAQ_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR0902902.1"
                     /translation="MENFSQSAPVNMAPTGIATFAKCPICPDIRQIQADIAVIGAPCD
                     MAIQGRPGARLGPRGIRTQSTRFRFSPQGSYDPERDDYYLSTEKWSVMDCGDIDYVPG
                     DLEQTFGNIEAAVRLLRSRGCLPVVLGGDHSISIPVARGLDQSGPFHVIQLDAHLDWT
                     DQLGGNRLFNGHPMRYMSQLPYVDRMAHLGIHGIGSSKRSDFQDARAYGATILSARQI
                     RAMGAQKVLEQLPQGGRYYVTIDIDAFDYSIAGGTGSPMLGGLLYDEAVDLLEGIAQL
                     GEVICVDLVEVSPPYDDAAGTTCYLAARILADFLGFITKERERRGER"
     gene            3083..3946
                     /locus_tag="ACZPAQ_02610"
     CDS             3083..3946
                     /locus_tag="ACZPAQ_02610"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR0902903.1"
                     /translation="MGAISRWLDRFCYKHPRLGIPGLMKYIVIANVAVYLMDMFSNGY
                     FSAMLRFYPALIMEGQVWRLLTFIFVPTSGGFILWFALTTLFYYYIGNALENQWGSTR
                     FTVFYGVGVLLNIFTGMVTYWISGAFLETASMYYVNMSMFFAFATLYPDMRVLLYGIL
                     PLKVKWLAWVDAALFAFDIGHYFMNGNWVQGMVPIVAILNYFIFFWDDLMAILRRGSS
                     RAAHRVDPQTINFKKAQKEVQNRKGYLHKCAVCGITDADDPNMEFRYCSKCNGYYCYC
                     MKHINDHVHIQ"
     gene            4108..4372
                     /gene="ffs"
                     /locus_tag="ACZPAQ_02615"
     ncRNA           4108..4372
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZPAQ_02615"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4435..>5426
                     /gene="dnaX"
                     /locus_tag="ACZPAQ_02620"
     CDS             4435..>5426
                     /gene="dnaX"
                     /locus_tag="ACZPAQ_02620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR0902904.1"
                     /translation="MYQALYRKWRPRTFDDVVGQSHITDTLKQQVAGGRLSHAYLFTG
                     TRGTGKTTCAKILSRAVNCQSPVNGNPCNQCPACLGIENGSILDVLELDAASNNGVDQ
                     VRALRDEAVYTPAAVRKRVYIVDEVHMLSTPAFNALLKILEEPPSHLMFILATTELHK
                     VPATIRSRCQQFAFKRILPGQIAQRLEYVAGQEGIDLTPEGAALLARLADGGLRDALS
                     LLDQCSGGGQQVDEQAVLDTLGLAGNLETARLMELAARHDTAAALETLSRLYAGGKDV
                     GAVLGELSSLARDLLMRKTAPRGGSALLTGGYDETTMRGLGELLTAPRLLQMLNR"
BASE COUNT          968 a         1706 c         1718 g         1034 t
ORIGIN      
        1 gaaggtgagc tccgggtcca tcacggcaaa gcgggggcgg cacaggtcgg tgttcaaccc
       61 ccgctttttc cagccgtcct cgttggtgat gacgcaggag ttgctcatct cactgccggc
      121 ggcggcgatg gtgagcacag tccccaccgg gaggcagtcc tgggcaggct ggccggagaa
      181 caggtcccac acgtccccct cgtaggccag ccccagggcg atggccttgg cggagtcaat
      241 gacgcttccg ccccccacgg ccagcaggaa gtccacaccc tcccggcggc acagctccat
      301 cccctcccgt accttggaca gccgggggtt agggaccacg ccgcccagct ccacgtgggc
      361 cacgccggcc cggtccagac tggccagcac ccggtccaga aggccgctct ccctggcgct
      421 gcggccgccg aaatggacca gcgccctggt cccgccctgc tccttcacca gctggcccgc
      481 ctgctcctcg gtgcccctgc cgaataccac cttagtgggg gtatagtact gaaagtcgtt
      541 catacagctc tcctttttac agtcgataag atgtgtctgc tggcagctct cccacaggag
      601 agcctgcccg ctgatgcagc cggccccggt tcaggcctcc cagcccgcca gataatcctt
      661 ctgctcctgg gtcagcgtat cgatggaaag ccccatggcg gccagcttca cccggccgat
      721 ctggtcgtcg atctcttcgg ggacgttgta gaccttcttc tccagctttc cggcgtgcct
      781 ggccatccac tccagggaaa gggcctggac ggcaaaggac atatccatga tctccgccgg
      841 gtggccgttt ccggcggcca ggttgaccag ccgcccctcc gccagcacgt tgagaacgcg
      901 gccgtcgggc aggcggaagc cctcgatgcc tgcccggcgg gggaagcgct cctccgccag
      961 ctggcccagg cgggccacat ctacctcaca gtcaaagtgc ccggagttgc acagcaggac
     1021 attgtgcttc atcacctgga agtggcgctc cacgatcacg tccttgcagc cggtggcggt
     1081 gacgaagata tcgccgtatc gggcggcctc gtccatgggc atcacccgga agttctccat
     1141 ggtggcctcc agggccttga aggggtccac ctcggtgacg atcacgttgg cccccatgcc
     1201 cttggcccgc atggcgatgc ccttgccgca ccagccgtag ccggccacca ccaccgtctt
     1261 gcccgctacc atcaggttgg tggtgtgcat gatggcgtcc caggtggact ggccggtacc
     1321 gtatttgttg tcgtagtagt gcttggcctt ggcgtcgttt accgccatca tggggcaggg
     1381 gaggatgccc gcccgctccc gggcgtacag gcggtggatg ccggtggtgg tctcctcaca
     1441 gcctcccagc agccggtccc cgtagggagc gcaccgcccc cccagcagag agatcaggtc
     1501 gccgccgtcg tccacgatca ggtgggggcg gcacttcagg gtctcggtca gcaggtcctc
     1561 atagccccgc atgtccaccc cgtggacacc gaaggtctcc acccccaggc tggccatggc
     1621 agcggccaca tcgtcctggg tggacagggg gttgcagccg gtgagataca cctgggcgcc
     1681 cccctcccgg agcaccagcc ccaggttggc ggtcttggcc tccaggtgga cagagacggc
     1741 gatggttagg ccctcaaagg gcttctcccg gcggaagcgc tcctcgatgc cgcccagggc
     1801 gggcatgaag tcccggaccc actggatctt ctgcaggccg gaaggggaca gagacaggtc
     1861 tttcacgatg ctcatatcat ttgctccttt gatttgatgg ggatggggat cagcgttctc
     1921 ccctgcgctc ccgctccttg gtgatgaagc ccaggaagtc ggccaggatc cgggcggcca
     1981 gatagcaggt ggtgcccgcc gcgtcgtcat aagggggcga gacctccacc agatccacgc
     2041 agatgacctc gcccagctgg gcgatgccct ccagcaggtc caccgcctcg tcatagagca
     2101 ggccgcccag catgggggag ccggtgccgc cggcgataga gtagtcgaaa gcgtcgatgt
     2161 cgatggtgac atagtagcgc ccgccctggg gcagctgttc cagaaccttc tgggctccca
     2221 tggcccggat ctggcgggcg gagaggatcg ttgccccata ggcccgggcg tcctggaagt
     2281 cggagcgctt gctgctgccg atgccgtgga tgcccaggtg agccatccgg tccacatagg
     2341 gcagctggga catgtagcgc atgggatggc cgttgaagag ccggttcccg cccagctgat
     2401 cggtccagtc caggtgggcg tccagctgga tcacatggaa ggggccggac tggtccagcc
     2461 cccgggcgac cgggatggag atggagtggt cgccccccag caccacgggc aggcagcccc
     2521 ggctgcggag caggcgcacc gcggcctcga tgttgccgaa ggtctgctcc agatcgccgg
     2581 ggacatagtc gatgtcgccg cagtccatca ccgaccactt ctcggtgctc aggtagtagt
     2641 cgtcccgttc tggatcatag gaaccctggg gactgaagcg aaagcgggtg gactgggtac
     2701 gtatgccccg ggggcccagc cgggcgccgg ggcggccctg gatagccata tcgcaggggg
     2761 cgccgatgac agcgatgtca gcctggatct ggcggatgtc agggcagatg ggacatttgg
     2821 caaaggtggc gatgccggtc ggggccatgt tgacaggggc gctctggctg aaattctcca
     2881 taaagaccat cctttcatgg gcgaaactaa aaaatttctt tctttacttt acaatgtttc
     2941 ggagacaaat tcaatgctga atgagaaaac tttccggccc atgggagaca aagacaaata
     3001 atttacagat ttgtcatgga caattcccga aaaccggggt acaatgaaaa acgatttgac
     3061 acaaatgtta ggaggctcct gtatgggcgc gatttcccgc tggctggacc gcttctgcta
     3121 caagcatccc cggctcggca tcccaggtct gatgaagtac atcgtcatcg ccaatgtagc
     3181 ggtctatctg atggatatgt tcagcaacgg ctacttctcc gctatgctgc ggttctaccc
     3241 ggccctgatc atggaagggc aggtgtggcg gctgctcacc tttatcttcg tccccaccag
     3301 cggcggtttc atcctgtggt ttgccttgac caccctgttc tactactaca tcggaaacgc
     3361 tctggaaaac cagtgggggt ctacccggtt caccgtgttt tacggggtgg gcgtcctgct
     3421 gaacattttc accgggatgg tgacttactg gatctctggt gccttcctgg agactgccag
     3481 catgtactac gtgaacatgt ccatgttttt tgccttcgcc accctgtacc cggatatgcg
     3541 ggtgctgctc tacgggatcc tgcccctgaa agtgaagtgg ctggcctggg tggatgcggc
     3601 cctctttgcc tttgatattg gtcattactt tatgaacggc aactgggtcc agggaatggt
     3661 ccccattgtg gccatcctga actacttcat cttcttctgg gacgatctga tggccatcct
     3721 ccgccggggg agcagccggg ctgctcaccg ggtcgatcct cagaccatca acttcaaaaa
     3781 ggctcagaag gaagtccaga accggaaggg ctatctccac aaatgcgccg tgtgcggcat
     3841 caccgacgca gacgacccca acatggagtt ccgctactgt tccaaatgca acggctacta
     3901 ctgctactgc atgaagcata tcaacgacca tgtgcacatc caatgagggc gggcgccccc
     3961 ggaccgacgg cccgggggcg cctttttgcg cgagagctgg ggcgctccct ccgatgggaa
     4021 aaggccgtga aaaatcctgt tgataaattc tgccggatgc agtataatat atcccagctt
     4081 tgttccagac ctcggtgtgg ggcaaggccg cactagtcgg ggagatagcg gtgccctgtt
     4141 cctgcaaccc gcttcagcag ggatgaatcc cggcccccgg acgtgcattg tgctgtctga
     4201 cctggataag cagcgatgat agaccggtcc cgcgcaacgc ggctccgtga accgtgtcag
     4261 gcggggaacc gagcagcact aagcggcccg ccgcgtgtgc cgcggggtta ctgttcttga
     4321 gccggctgtc caggtaacgg gtatagtgta cgcttcaaag gccggtgtgc ggccttgctc
     4381 tgcatcgagt attttttgcc cggatgggca gagaaaagga tttgaggtga tatcgtgtat
     4441 caggccctgt accgcaaatg gaggccccgc acttttgacg atgtggtggg ccagagccat
     4501 atcaccgaca ctttgaagca gcaggtggcg ggggggcggc tgtcccacgc ctatctgttc
     4561 actgggaccc gggggacggg caagaccacc tgcgccaaga tcctgtcccg ggcggtgaac
     4621 tgccagagcc cggtgaatgg caacccctgc aatcagtgcc cggcctgcct gggcattgaa
     4681 aacggttcta tcctggatgt gctggagctg gatgccgcct ccaacaacgg cgtggaccag
     4741 gtccgggccc tgcgggatga ggcggtctat accccggcgg cggtccgtaa gcgggtctat
     4801 atcgtggacg aggtccatat gctctccacc ccagccttca atgcgctgct gaagatcctg
     4861 gaggagccgc cgtcccacct gatgtttatc ctggccacca cggagctgca caaggtgccg
     4921 gctaccatcc gctcccggtg tcagcagttc gcgttcaaac gcatcctgcc cggacagatc
     4981 gcccagcgcc tggagtatgt ggcggggcag gagggcatcg acctgacccc ggagggagcg
     5041 gcccttctgg cccggctggc tgatggcggc ctgagggacg ccctttccct gctggaccag
     5101 tgctccggcg gcggacagca ggtggacgag caggcggtgc tggataccct ggggctggcg
     5161 ggaaatctgg agaccgcccg gctgatggag ctggccgccc ggcacgacac tgccgccgcc
     5221 ctggagaccc tgtcccggct gtacgccggg ggaaaggacg tgggggcggt gctgggggag
     5281 ctgtcctccc tggcccggga tctgctgatg agaaagacgg cccccagagg cggcagtgcc
     5341 ctgctgaccg gtggatatga cgagaccacc atgcgcggcc tgggagagct gctcaccgcg
     5401 ccccgcctgc tccagatgct caaccg
//
LOCUS       DBMDSJ010000067         5426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4239_length_5426_cov_1.697277, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000067 DBMDSJ010000000
VERSION     DBMDSJ010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4239_length_5426_cov_1.697277"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="ACZPAQ_02625"
     CDS             complement(<1..622)
                     /locus_tag="ACZPAQ_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR0902905.1"
                     /translation="MNRKMTQMLQPSFQLYFVCLILFAAASAFFSLYLAAAEAVVVVL
                     LGLYYRENSRRRRREIAKYIENVTGNMDVATKDTMVNSPLPMVIFRPESDDIIWTNDR
                     FLHLAGEKEHLFDAKLSSVIPDFDTRWLMEGKNECPTEVTFGPRRFLVYGHLVRASGR
                     EGGFLATTDWVDVTEFCQVRDQFRGSRPVMAVLLLDNYEDLMKNQTE"
     gene            994..2058
                     /locus_tag="ACZPAQ_02630"
     CDS             994..2058
                     /locus_tag="ACZPAQ_02630"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR0902906.1"
                     /translation="MQEIKITRAEQRKEKPDSSKLVFGKYMTDHMFVVDYDEGQGWHD
                     ARIVPYAPLQIDPAAKVLHYAQEIFEGLKAYRTADGSIQLFRPMDNVRRLNLSCERIA
                     LPEVPEDLALAGITELVKLDQEWVPYEKDTSLYIRPFVIGLDPTLGVHTSHHCQYIVI
                     VCPVGAYYPEGLAPTKIYIEDQDVRAVRGGTGMAKTGGNYAASLRAGNRAEQKGYTQV
                     LWLDGVHRKYIEEVGAMNVMFKVNGKILTPDLNGSVLDGITRRSCIQLLKDWGYEVEE
                     RRISAEELFQAAKDGTLEEAWGTGTAAVVSPIGELAEGDEKAIINHNEIGPVTQKLYD
                     TLTGIQWGRVPDPHGWIMKL"
     gene            complement(2140..2955)
                     /gene="murI"
                     /locus_tag="ACZPAQ_02635"
     CDS             complement(2140..2955)
                     /gene="murI"
                     /locus_tag="ACZPAQ_02635"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR0902907.1"
                     /translation="MFSKESPIGIIDSGIGGFSVARKVQKLLPRENLVYLGDGANTPY
                     GNHTAEEILTMTRYMLRFMEEKGVKALLVACNTISCLIDQYRDEMSCPVLSVVQAGAD
                     AVKDLDVHKVGVISTCFTASTHCYPDLIGKAAPDKVVISHGCPDLANVVERNVGRPEG
                     QALIDADVQKNLEGLVRDEQIECCVLGCTHYPLVEENIHRLFPGLRLLDPAEQMAKDV
                     LAYLERDGLVNDGPQPGRLDIFTTGSAEEYAMKAEKVGLDPVTSVQFYPPMKL"
     gene            complement(3023..3880)
                     /locus_tag="ACZPAQ_02640"
     CDS             complement(3023..3880)
                     /locus_tag="ACZPAQ_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902908.1"
                     /translation="MAWPKIKNIIILMLLVTNLCLLAFVGWREIRDWQLQDKALEQAV
                     SFLAERGIQVDERIVPRSAELPPMGMERDLEEEQTLAAALLGDGLTREERSGEVYVYE
                     GERGLLQCHSSGEFWADFQSGAFPVPEGAEMAEHALETMELLHFQGEVVSSAGNAQDG
                     SVTLRQLWNGVPVLPCRATLVYEGGELKRIEEGRRLTGTPQTTAGTPITATTALMRFY
                     AGLSERMDICSEVREIEIAYTLSNALGGPISLIPVWHITTDTEIYELNTLDGSLAAAG
                     AEETERTEQ"
     gene            complement(3906..5213)
                     /locus_tag="ACZPAQ_02645"
     CDS             complement(3906..5213)
                     /locus_tag="ACZPAQ_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902909.1"
                     /translation="MNRKSKHARLLELGKDVLIVLLACSAVWLTTRVQRTGSLGDLLA
                     DTPQEGQGQTQAPGQMEMVRPVRMAAVGRSGETTIRYGAQYDQQSTDALFQQVANLLV
                     EALSDGGEMSPVSQWEWRTALTSPPSLYFDLMEGVPLSVLTGWLTGESTGGEVFVRRL
                     ALAASRGQVFLYYQDEESGQFYAGPVDVISVSRLESAVSGLSDNGACFAFEKEGYSLL
                     SPYTLLMPGTPQPAVYQVYNPLSREEGLNSLLEALDFPASSSYTYQGTDGELVVRNGY
                     DTLRVSAQGTVRYHTTEGEISRYPVASDTGGTGCYEAVEACRVLVSETLGTQCGAARL
                     VLTHVERITGGWAVEFGYCLDGAAVQVYEEGCAARFLVQNGQIAEITLQARSYEETAE
                     SGLVLPRLQAMAAMEAAGLEGRELLLGYQDSGGGTVTAGWIAR"
     gene            complement(5206..>5426)
                     /locus_tag="ACZPAQ_02650"
     CDS             complement(5206..>5426)
                     /locus_tag="ACZPAQ_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0902910.1"
                     /translation="GIGIPPEDRPRIFERFYRVDKARSRQSGGTGLGLSIAKEIIDRH
                     QGVLELADKEGPGLAVRMELKIEGPDHE"
BASE COUNT          938 a         1912 c         1557 g         1019 t
ORIGIN      
        1 tctccgtctg gttcttcatc aggtcctcat agttgtccag cagcagcacc gccatcaccg
       61 ggcggctccc ccggaactgg tcccgcacct ggcagaactc cgtcacgtcc acccagtcgg
      121 tggtggccag gaagcccccc tcccggcccg aggcccggac caggtgtccg tacaccagaa
      181 agcggcgggg accgaaggtg acctcggtgg ggcactcgtt tttcccctcc atgagccagc
      241 gggtatcaaa atccgggatc acgctgctca gcttggcgtc gaacaggtgc tccttctccc
      301 ctgccagatg gagaaaacgg tcattggtcc agatgatgtc gtcgctctcc ggccggaaga
      361 tcaccatggg caggggggag tttaccatgg tgtccttggt ggccacatcc atgttcccgg
      421 tgacgttctc gatgtacttg gctatctccc gccgccgccg gcggctgttc tcccggtaat
      481 acagccccag caggaccacc accacggcct ccgccgccgc cagatacagg ctgaaaaagg
      541 cggatgccgc ggcaaacagg atcagacaga cgaaatagag ctgaaagctt ggctgaagca
      601 tctgcgtcat ttttcggttc aagatccgtt cctcctcttc ggggcggccg ttcctccccc
      661 tcccgggggc gggacctcct atcacaacaa aaaggtccgc ccgtcagcag gaccttcttc
      721 acgccccatt cccgcgccgt gccgccgtat cagcgcggtg attggtgata ttatatcaaa
      781 cccccagggg aaaaacaata gcaaaccgtc caggcggggc cattctctgg ggaaatggcg
      841 gaattctttc cctaaaaagt tgggccggcc ccacttttcc cacgattacc cgttgcgtct
      901 gaaaaaaaag gctgatataa tatggaaata ccagaaaagc cgtcctccaa accaggaccg
      961 ctcgatttga gtttacttta ggaggaaacc aagatgcaag agatcaagat cacccgcgcc
     1021 gagcagcgga aggagaagcc tgactcctcc aagctggtct tcggcaagta tatgaccgac
     1081 cacatgtttg tggtggacta tgacgagggt cagggctggc atgacgcccg catcgtcccc
     1141 tatgcccccc tccagatcga cccagccgcc aaggtcctcc attacgccca ggagatcttc
     1201 gagggtctga aagcctaccg caccgcagac ggctccatcc agctgttccg ccccatggac
     1261 aacgtgcgtc ggctgaacct gtcctgcgag cgcattgccc tgcccgaggt gcctgaagac
     1321 ctggccctgg ccggtatcac tgagctggtc aagctggatc aggagtgggt cccctatgag
     1381 aaggacacct ccctgtatat ccgtcccttt gtcattggtc tggaccccac cctgggcgtc
     1441 cacacctccc accactgcca gtatatcgtc atcgtctgcc ctgtgggcgc ctactacccc
     1501 gagggactgg cccccaccaa gatctatatc gaggaccagg acgtccgcgc cgttcggggc
     1561 ggcaccggca tggccaagac cggcggcaac tacgccgcct ccctgcgggc gggcaaccgc
     1621 gccgagcaga agggttacac ccaggtcctg tggctggacg gcgtccaccg caaatatatt
     1681 gaggaagtag gcgctatgaa cgtcatgttc aaggtgaacg gcaagatcct cacccccgac
     1741 ctgaacggtt ccgtgctgga cggcatcacc cgccgctcct gcatccagct gctgaaggac
     1801 tggggctatg aggtggagga gcgccgcatc tccgccgagg agctcttcca ggccgccaag
     1861 gacggaaccc tggaggaggc ctggggcacc ggcaccgctg ctgtggtctc ccccatcggc
     1921 gagctggccg agggggacga gaaggccatc atcaaccaca atgagatcgg ccctgtcacc
     1981 cagaagctgt acgacaccct caccggcatt cagtggggcc gcgttcccga tccccacggc
     2041 tggatcatga agctgtaagc agatttttta aactcaaggg ccgccccggt ggggcggccc
     2101 ttgagccttt tttctgacgc cggctccgcc ggactatttt tacagtttca tggggggata
     2161 gaactgcacc gaggtcaccg ggtccagtcc caccttctcc gccttcatgg cgtactcctc
     2221 cgcgctgccg gtggtgaaga tgtccagccg tccgggctga gggccgtcgt tcaccaagcc
     2281 gtcccgctcc agataggcca acacgtcctt ggccatctgc tccgccgggt ccagcagccg
     2341 caggcccggg aagagcctgt ggatattttc ctccaccaag ggatagtggg tgcagcccag
     2401 cacacagcat tcgatctgct cgtcccggac cagtccctcc aggttcttct gtacatctgc
     2461 atcgatcagc gcctgtccct ccggcctgcc cacgtttcgc tccaccacgt tggccaggtc
     2521 agggcagcca tggctgatga ccaccttgtc cggcgctgcc ttgccgatca gatccgggta
     2581 gcagtgagtg gaggcagtga agcaggtgga gatgactcct accttatgga catccaagtc
     2641 ctttaccgcg tccgcacccg cctggactac gctgagcacc gggcagctca tctcatcccg
     2701 gtactgatca atgaggcagg aaatggtgtt gcaggccacc agcagggcct tgaccccctt
     2761 ttcttccatg aagcgcagca tgtagcgggt catagtgagg atctcctccg cggtgtggtt
     2821 gccatagggc gtgttggccc cgtcccccag atagaccaga ttctcccgcg ggagcagctt
     2881 ctgcaccttc cgggccacgc tgaagccgcc tatccccgaa tcgatgatcc caatggggct
     2941 ttctttgctg aacatgatga tctccttctt tctttgttgt cccgctccgg cccttccggc
     3001 cgggcatgtc agtggtctcg ttctactgct ccgtccgctc tgtctcttcg gcgcctgccg
     3061 cagccaggct cccgtccaag gtgttcagct catatatctc ggtgtcggtg gtgatgtgcc
     3121 agaccgggat cagcgagatg ggacccccca gggcgttgga gagggtatag gcaatctcga
     3181 tctcccggac ctcactgcag atgtccatcc gctcgctcag gcccgcataa aagcgcatca
     3241 gcgccgtcgt cgccgtgatg ggcgtacctg ctgtggtctg gggcgtcccg gtgagacgcc
     3301 ggccctcctc gatccgcttc agctcgcccc cctcgtagac caaggtcgcc cgacagggca
     3361 gcaccggcac gccgttccac agctgccgca gggtgacgct cccgtcctgg gcgttgcccg
     3421 cgctgctcac cacctccccc tggaagtgga gcagctccat ggtctccaga gcatgctccg
     3481 ccatctccgc cccctctggg accgggaagg cgccgctctg aaagtcggcc cagaactccc
     3541 cgctgctgtg gcactggagc agcccccgct ccccctcata gacatagacc tcgccgctgc
     3601 gttcctcccg ggtcaggcca tcccccagca gcgctgctgc cagggtctgc tcttcctcca
     3661 ggtcccgttc catgcccatg ggcggcagct ccgcagacct gggcacgatg cgctcgtcca
     3721 cctggatgcc ccgctccgcc aggaaggaga cggcctgctc cagcgccttg tcctggagct
     3781 gccagtcccg gatctcccgc cagcccacaa aggccagcag gcacaggttg gtcaccagca
     3841 gcatcaggat gatgatattt ttgatcttgg gccaggccac ggctctccct cccctccggt
     3901 ctctctcacc gggcgatcca gccggcggtg accgttccgc cgccgctgtc ctgatatccc
     3961 agcagcagct cccggccctc cagccccgcc gcctccatgg cggccatggc ctggagccgg
     4021 ggcagcacca gcccgctctc cgccgtctcc tcatagcttc tggcctggag ggtgatctcc
     4081 gcgatctgtc cgttctgcac caggaaccgg gccgcgcatc cctcctcgta cacctggact
     4141 gccgcgccgt ccagacagta gccgaactcc accgcccagc cccccgtgat ccgctccaca
     4201 tgggtgagca ccagccgggc ggccccgcac tgggtgccca gtgtctcgga gaccagcacc
     4261 cggcaggcct ctaccgcttc atagcacccc gttccgccgg tgtccgaggc caccggataa
     4321 cgggatatct ccccctccgt agtatggtag cgcaccgtgc cctgggcaga tacccgcagg
     4381 gtgtcatacc cgttgcgcac caccaactct ccgtcggtcc cctgataggt gtagctgctg
     4441 ctggccggga agtccagtgc ctccagcagg ctgttgaggc cctcctcccg gctgaggggg
     4501 ttgtagacct gatagaccgc cggctggggt gtgcccggca tcagcagggt gtagggagac
     4561 aggaggctgt acccctcctt ctcaaaggca aaacaggcgc cgttgtccga caggccggag
     4621 acagcggact ccagccggct gacgctgatc acgtccaccg ggccggcgta aaactgcccg
     4681 ctctcctcat cctggtagta gaggaacacc tgtccccggg aggcggccag tgccagccgg
     4741 cgcacgaaca cctccccccc tgtgctctcc ccggtgagcc agcccgtcag caccgacagg
     4801 gggaccccct ccatcaggtc gaagtacagg ctgggcgggg aggtcagggc ggtgcgccac
     4861 tcccactggc tgacggggct catctctccc ccgtcgctca gcgcctccac cagcagattg
     4921 gccacctgct ggaatagggc gtcggtgctc tgctggtcat actgtgcccc atagcggatg
     4981 gtggtctctc cgctcctgcc cactgccgcc atccgtaccg ggcggaccat ctccatctgg
     5041 ccgggggcct gggtctggcc ctgcccctcc tggggcgtgt ccgccagcag atctcccaag
     5101 ctcccggtgc gctggaccct ggtagtcagc cacaccgctg agcaggccag caggacgatc
     5161 agcacgtcct tgcccagctc cagcaggcgg gcgtgcttgc tctttctatt catggtccgg
     5221 cccctctatc ttcagctcca tccgcaccgc cagtcccggg ccctccttgt cggccagctc
     5281 cagcacgccc tggtgccggt cgatgatctc cttggcgatg gacaggccca gtccggtccc
     5341 gcccgactgg cgggaccggg ccttgtccac ccgataaaac cgctcgaaga tgcggggccg
     5401 gtcctccggc gggatgccga tgccgt
//
LOCUS       DBMDSJ010000068         5415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4244_length_5415_cov_2.460294, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000068 DBMDSJ010000000
VERSION     DBMDSJ010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4244_length_5415_cov_2.460294"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2734
                     /locus_tag="ACZPAQ_02655"
     CDS             <1..2734
                     /locus_tag="ACZPAQ_02655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR0902911.1"
                     /translation="QADSLTVTLTCDHLEEDEGGAGIFSPARRTAAGLLRLAAQEGIS
                     CEVEHLVPDWDKRCEPLRQVEKQLFAHGEAEPVSCQRRVELLGALTPRSEVEQAAAGI
                     RRLLRTQGLRCRDIGVTARDLSVYQPLVEAIFPRYQIPVFTSAMTDILDKPILTLVTA
                     ALDTVANGYRYDDVFRYLKTGLTDLPEEDRDLLENYVLKWNLRGSQWTQKKPWAMPPK
                     GYGAAVTDEDRALLERLDRARRQVAEPLELLRKNQNRTGVGQAICLYSFMEEIGLPQR
                     LEDRVAELHRREQPALAEEYRQLWEILCRGLEQCAALLGETPMELEEFSGLFRLVLSQ
                     YDVGTIPVSLDRVTVGETTRETGHPVKVLFLLGADDVSIPKAGSAPGLLSDEDRSVLA
                     GYGLELAQSSQELLYREMTTVYLTCARPSERLVVSWPSQSAAGEERRPSFLVERLRRL
                     FTDLRVTREEDSGGAFRLEAPLPALEQAGRSPAARRALAALPEYAPMVERLDRAAGWS
                     RGRLSRLAVDQLYGRKVSMSASRMDKYKSCHFSYFMRYGLQAEPRKPAGFTAPEYGTF
                     VHYVLEHVLRDEMFRQTVLPGFSDPVAGAEGRRRLRELTRAAVDRYVEEELGGLEHQT
                     ERFRYLFRRLLRSVQAVVDNVAEELAASQFRPISFELGFGSGQDLPPVELTVDGVTIS
                     VTGFVDRVDGWVHDGRLYLRVVDYKTGKKSFDLTEVWNGLGLQMLLYLFALEARGPDY
                     YGQPVEGAGVLYLPARDAVVKGSRSMTDEAWRKQLDRELTRSGLVLDDPAVLSAMEEP
                     GETGYRFLPLKVSKSTGAVSGEALATAERLGRLGGHLQRTLEEICRELAEGNIAADPF
                     WRGPEKNACRFCDYAAACLFEEGRGGDCRRWLPSVKSRDFWKQLEEETDGEDGA"
     gene            2731..3948
                     /locus_tag="ACZPAQ_02660"
     CDS             2731..3948
                     /locus_tag="ACZPAQ_02660"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIR0902912.1"
                     /translation="MTDRVYKLEIFLPQSCFDAVRQALWSVDAGHIGAYDRCLSWSRV
                     ESCWRPLEGTDPFLGTPGELCWAEEWKVEVCCREDRLAETLAAARAAHPYEEPVINVI
                     PLAATGLEEKKQGWTPAVSRTRSKIAMENNKNILIINDMPGYGKVATAAMLPILSNMG
                     HSVYNLPTALVSNTLDYGTFSILDTTDYMVQAIDAWKKLGFSFDCITTGFLASAEQVD
                     IIREFIASQKKEGLLVMTDPIMGDDGKLYNGVTEETVENMRRLLGVADVIVPNLTEAE
                     FLTGKYQGRESLTAGEARELVDGLLTFGPRSVVVTSGVEEETGRHVVWGYNRETGAHF
                     TIPYQFIQVHFPGTGDMFSAVLVGELLAGRGLEPAVRRAMDVLETLIRLERDEAEKNK
                     GIRIEKYLSVLNQ"
     gene            3963..4376
                     /locus_tag="ACZPAQ_02665"
     CDS             3963..4376
                     /locus_tag="ACZPAQ_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR0902913.1"
                     /translation="MWAGFALLSALFAALTSILAKLGMEGINSNLATAIRTAVVLVMA
                     WGIVFLTGKHHGIGDIGGRSWIFLILSGVATGLSWLCYYRALQLGEASRVVPIDKFSV
                     VISMVLAFVVLKEAVTVKTVVGGLLITAGTLVLIL"
     gene            4417..>5415
                     /gene="holA"
                     /locus_tag="ACZPAQ_02670"
     CDS             4417..>5415
                     /gene="holA"
                     /locus_tag="ACZPAQ_02670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR0902914.1"
                     /translation="MPPKGKPDTAGYQRFKKELAAGTPGKLYLLHGEETYLRDHYLGK
                     LRSLLLTGGMGTFNLHEIPAREMSPRRLEEALDCLPMMAERTLVLVTDFDLFKAGEKD
                     REEYIRLLGDLPDYVCLVFVYDLIPYKGDARTKLAGAIRQNGVVVNFARQDQGDLVDW
                     VRRRFRALGKDIDSRLALDLIFLCGDLMTNLIGEIEKVGAYAKGQRITRGDIDAVATP
                     QLDAVVFRMTDAIGEGKFDRAAAVLGELYQMQEPPIKIMWSLGRQMRQLYSARLVMEQ
                     GKGAQYLAGLWGLKPYPVEKLMGAARRFTLPWCRRAVVRCGEVDLAMKSTGQDGQQL"
BASE COUNT          861 a         1621 c         2042 g          891 t
ORIGIN      
        1 tcaggcggac agcctcacgg tgaccctcac ctgcgaccat ctggaggagg acgagggagg
       61 cgcggggatc ttctcacccg cccgacgcac ggcggccggg ctgctccggc tggcggcaca
      121 ggaggggatt tcctgtgaag tggaacacct tgtcccagat tgggataaga ggtgcgagcc
      181 cctccggcag gtggagaagc agctgtttgc ccatggggag gcggagccgg tctcctgcca
      241 gaggcgggtg gagctgttgg gggcgctgac cccccggtca gaggtggagc aggcggccgc
      301 tgggatccgc cgcctgctgc ggacccaggg cctgcgctgc cgggacatcg gggtgacggc
      361 ccgggacctg tcggtctatc agccgctggt cgaggccatc ttcccccgct atcagatccc
      421 ggtgttcacc tccgccatga cggacatcct ggacaagccc atcctcaccc tggtgacggc
      481 ggcgctggac acggtggcca acggctaccg ctatgacgat gtgttccgct atctcaagac
      541 cggcctcacc gatctgccgg aggaggaccg ggacctgctg gagaactatg tgctgaaatg
      601 gaacctccgg gggagccagt ggacccagaa aaagccctgg gccatgcccc ccaaggggta
      661 cggggccgcc gtcaccgatg aggaccgggc gctgctggag cggctggacc gggcccgccg
      721 ccaggtggcg gagccgctgg agcttttgcg gaaaaatcag aaccggaccg gggtggggca
      781 ggcgatctgc ctttacagct ttatggagga gatcggcctg ccccagcggc tggaggatcg
      841 ggtggcggag ctgcaccgcc gggagcagcc cgccctggcg gaggagtacc ggcagctgtg
      901 ggagatcctg tgcaggggcc tggagcagtg cgccgccctt ctgggggaga cccccatgga
      961 gctggaggag ttctccggcc tgttccgcct ggtactctcc cagtacgatg tgggcaccat
     1021 ccctgtgtcc ctggaccggg tgacggtggg ggagaccacc cgggagacgg gacacccggt
     1081 gaaggtcctc ttccttctgg gggcggacga cgtctccatc cccaaggcgg gcagcgcccc
     1141 ggggctgctg tccgacgagg accgctcggt tctggcgggc tacggcctgg agctggctca
     1201 gtcctcccag gagctgctct accgggagat gaccacggtg tacctcacct gcgcacggcc
     1261 gtcggagcgg ctggtggtca gctggcccag ccagagcgcc gccggggagg agcgccgccc
     1321 cagctttctg gtggagcggc tgcggcggct gttcaccgat ctgcgggtga cgcgggagga
     1381 ggactccggc ggtgcgttcc gtctggaggc ccctctgccc gccctggagc aggcggggcg
     1441 gagcccggcg gcccggcggg ccctggccgc tctgccggag tacgccccca tggtggagcg
     1501 gctggaccgg gcggcgggct ggagccgggg ccggctgtcc cggctggcgg tggaccagct
     1561 gtacgggcgg aaggtgtcca tgtcggcctc ccggatggac aagtacaagt cctgccattt
     1621 ttcctatttc atgcgctatg gcctccaggc ggagccccgc aagccggctg gctttacagc
     1681 accggagtac gggacctttg ttcactatgt tttggaacac gtcctccggg atgagatgtt
     1741 ccggcagacc gttctgcccg gtttttccga cccggtggcc ggggcggagg gacgccgccg
     1801 cctgcgggag ctgacccggg ccgcggtgga ccggtatgtg gaggaggagc tgggcgggct
     1861 ggagcaccag acggagcgct tccgctatct gttccgccgg ctgctccggt cggtgcaggc
     1921 ggtggtggac aatgtggcgg aggagctggc cgcctcccag ttccgcccca tctccttcga
     1981 gctgggcttc ggctccgggc aggacctgcc cccggtggag ctcaccgtgg acggggtgac
     2041 catcagcgtc accggctttg tggaccgggt ggacggctgg gtccacgacg ggcggctcta
     2101 cctgcgggtg gtggactata agacggggaa gaagtccttc gacctgacgg aggtgtggaa
     2161 cgggctgggc cttcagatgc tgctctacct gtttgccctg gaggcccggg ggccggacta
     2221 ctacggccag ccggtggagg gggccggggt gctctatctc cccgcccggg acgcggtggt
     2281 caaggggagc cgctccatga ccgacgaggc gtggcggaag cagctggacc gggagctcac
     2341 ccgcagcggc ctggtgctgg acgatccagc ggtgctgtcc gccatggagg agccggggga
     2401 aacgggctac cgtttcctgc ccctgaaggt gtccaagtcc accggggccg tctccgggga
     2461 ggccctggcc acggcggagc ggctgggcag gctggggggc cacctccagc gaacgctgga
     2521 ggagatctgc cgggagctgg cggaggggaa catcgccgcc gatcccttct ggcgggggcc
     2581 ggagaaaaac gcctgccgct tctgcgacta cgccgccgcc tgcctctttg aggagggccg
     2641 ggggggcgac tgccgccgct ggctgcccag tgtgaagagc cgggatttct ggaagcagct
     2701 ggaggaggag acggacgggg aggacggagc atgacggacc gggtgtacaa gctggagatc
     2761 tttctgcccc agagctgctt tgatgcggtg cggcaggccc tgtggtcggt ggacgcgggc
     2821 cacatcgggg cctatgaccg gtgcctgtcc tggagccggg tggagagctg ctggcggccc
     2881 ctggagggga cggacccctt tctggggacg ccgggggagc tgtgctgggc ggaggagtgg
     2941 aaggtggagg tctgctgccg ggaggaccgg ctggcggaga ccctggccgc cgcgcgggcg
     3001 gcccacccct atgaggagcc ggtgatcaat gtgatccccc tggccgccac cggcctggag
     3061 gagaaaaaac agggttggac acctgcggtg tcccggacaa ggagtaagat tgccatggaa
     3121 aacaacaaaa atattctcat catcaacgat atgccgggct atggcaaggt ggcaacggcg
     3181 gccatgctgc ccatcctgtc caacatgggg cacagcgtgt acaacctgcc caccgccctg
     3241 gtgtccaaca cgctggacta tgggaccttc agcatcctgg acaccaccga ctacatggtg
     3301 caggctatcg atgcctggaa gaagctgggg ttctcctttg actgtatcac cacgggcttt
     3361 ctggcctcgg cggagcaggt ggacatcatc cgggagttca tcgccagtca gaagaaggag
     3421 ggccttctgg tgatgaccga ccccatcatg ggggacgacg ggaagctcta taacggggtg
     3481 acggaggaga cggtggagaa tatgcgccgg ctccttgggg tggcggatgt gatcgtcccc
     3541 aacctgacgg aggcggagtt cctcaccggg aagtaccagg ggcgggagtc cctcaccgcc
     3601 ggggaggccc gggagttggt ggatggcctg ctgaccttcg gcccccggtc ggtggtggtg
     3661 accagcggcg tggaggagga gaccggccgc catgtggtct gggggtataa ccgggagacc
     3721 ggggcccact tcaccatccc gtatcagttc atccaggtcc acttcccggg tacaggggat
     3781 atgttctccg ccgtgctggt gggggagctg ctggccggcc ggggtctgga gccggcggtg
     3841 cgtcgggcca tggacgtgct ggagaccctg atccggctgg agcgggacga ggcggagaaa
     3901 aacaagggca tccgcatcga aaagtacctc agcgtactga accagtgacg gggtgcggat
     3961 gtatgtgggc tggattcgcg ctgctgtcgg cgctgttcgc cgcgctgacc tccatcctgg
     4021 ccaagctggg gatggagggg atcaactcca acctggccac ggctatccgc acggcggtgg
     4081 tcctggtgat ggcctggggg atcgtctttc tcacggggaa gcaccacggc atcggggaca
     4141 tcgggggccg cagctggatc ttcctgatcc tgtccggggt ggccaccggc ctgtcctggc
     4201 tgtgctacta ccgggcgctc cagctggggg aggccagccg ggtggtccca atcgacaagt
     4261 tcagcgtggt catctccatg gtgctggcct ttgtggtgct gaaggaggcg gtgaccgtca
     4321 agaccgtggt gggcgggctg ctgatcaccg cggggaccct ggtgctgatc ctgtgaccgg
     4381 ggccggcctg aaatcgaacg cataggagga atgtagatgc cgcccaaggg gaagccggat
     4441 accgccggct atcagcggtt taaaaaggag ctggccgcgg ggacgccggg gaagctgtat
     4501 cttctgcacg gggaggagac ctatctccgg gaccactacc tggggaagct gcggtccctg
     4561 ctgctcaccg gagggatggg gaccttcaat ctccatgaga tcccagcccg ggagatgtcc
     4621 ccccgccgcc tggaggaggc gctggactgc ctgcccatga tggcggagcg caccctggtc
     4681 ctggtgaccg acttcgacct gttcaaggcg ggggagaagg accgggagga gtatatccgg
     4741 ctgctgggcg acctgccgga ctatgtgtgt ctggtgttcg tgtacgacct gatcccctat
     4801 aagggggacg cccggaccaa gctggccggg gccatcaggc agaacggcgt ggtggtcaac
     4861 ttcgcccgcc aggaccaggg ggatctggtg gactgggtgc gccggcggtt ccgggccctg
     4921 gggaaggaca tcgactcccg tctggccctg gacctgatct tcctgtgcgg ggatctgatg
     4981 accaacctca tcggggagat cgagaaggtg ggggcctatg ccaagggcca gcggatcacc
     5041 cggggggaca tcgacgcggt ggccaccccc cagctggacg cggtggtgtt ccgcatgacc
     5101 gacgccatcg gcgaggggaa gttcgaccgg gcggcggcgg tgctggggga gctgtaccag
     5161 atgcaggagc cacccatcaa gatcatgtgg tccctggggc ggcagatgcg gcagctctac
     5221 tccgcccgcc tggtcatgga gcaggggaag ggggcccagt atctggcggg gctgtggggg
     5281 ctcaagccct atcccgtgga gaagctgatg ggggccgccc gccggttcac tctgccctgg
     5341 tgccgccggg cggtggtgcg ctgcggcgag gtggacctgg ccatgaagtc caccggacag
     5401 gacgggcagc agctg
//
LOCUS       DBMDSJ010000069         5354 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4301_length_5354_cov_1.493242, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000069 DBMDSJ010000000
VERSION     DBMDSJ010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4301_length_5354_cov_1.493242"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="ACZPAQ_02675"
     CDS             <1..628
                     /locus_tag="ACZPAQ_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dehydroquinate synthase/iron-containing alcohol
                     dehydrogenase family protein"
                     /protein_id="HIR0902915.1"
                     /translation="PRFSILNPRFTLTLPREQMVAGIYDIFNHICEQYFSGEDDNTSD
                     YLSEGLMRSLLCSSRAANRDPQDYEARSNIMWTATWALNTLVSRGKSTDWMVHMLGQA
                     VGGYTNATHGMTLAAVSLPYYRHILPYGLDKFKRFAVQVWGVDPAEEGLAAMEGWMKE
                     LGLTMNTSALGVTEDMLEGLADVTIHLTGGYKDLDREEILQIFRESLG"
     gene            complement(693..1484)
                     /locus_tag="ACZPAQ_02680"
     CDS             complement(693..1484)
                     /locus_tag="ACZPAQ_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HIR0902916.1"
                     /translation="MSWQQVCFDYDGSYPGFLTCVFESYAHREEAVCFTSPEEDRLTL
                     WPERHVDTDQAKAERVYRSLSKKISPQACRLVTQAFLTCLPERELHMYRFLRLGYQRG
                     ADITRDLTDDRVHVLWKAVQHLGSEAHLLKGFLRFSDQQGVLVGEIEPKNRVLPLLRP
                     HFCTRYSEEHFLIYDHTHRELLIHQPGRWAILPAEDFAPAPPGQEEVTYRRLWRRFYD
                     TIAIRERENPRCRMTNMPKRFWGTMTEFQPDEGALPAPGPRGKGW"
     gene            complement(1481..2752)
                     /locus_tag="ACZPAQ_02685"
     CDS             complement(1481..2752)
                     /locus_tag="ACZPAQ_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR0902917.1"
                     /translation="MDLMDKLTILADAAKYDAACTSSGLDRAGRAGRLGSTMAAGCCH
                     SFSADGRCISLLKILYTNICAYDCQYCVNRRSNDPPRAAFTPRELAELTIQFYRRNYI
                     EGLFLSSAVLRDPDYTTERMIEALRLLRQDYGFGGYIHAKAIPGADPLLTQQLGLLAD
                     RLSVNIELPSAKSLALLAPDKGRAGILAPMAQIRDGITQSRQELAKYHHAPRFAPAGQ
                     STQMIVGATPESDRHILMLTQGLYDKYHLKRVFYSAYIPVSSSSLLPAPQGFQPPLLR
                     EHRLYQADWLLRFYHFRAEELLDEGRPDFDLRLDPKCGWALRHLDQFPVEVQRADYEA
                     LLRVPGIGVTSAKRILTARRAGPLSFSGLKKLGVVLKRAQYFITCSGRMLEGLRVRED
                     GILSHLAALDRPALAQVQPEQLSLFGEEGAS"
     gene            complement(2918..3424)
                     /locus_tag="ACZPAQ_02690"
     CDS             complement(2918..3424)
                     /locus_tag="ACZPAQ_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902918.1"
                     /translation="MKSVILLLMILGVGGWVLSHSASYIAHRRAAHGPLTRDFFHGNS
                     EIPPAFRQALEETIVFLDNRRDWAAGSALDAGTTDLCWIEIKDCDLLLRAGLFSPGNL
                     GRLQIRRRLPLLPPGALPLSGSEAAQLTHFLLAALQVYYRGAPEVTSAGPRCLRADFS
                     PLHGTPEH"
     gene            3553..3855
                     /locus_tag="ACZPAQ_02695"
     CDS             3553..3855
                     /locus_tag="ACZPAQ_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0902919.1"
                     /translation="MSMETRALGDAIRAARMKKGLTQDALSELLDITPTHLKNIEGSR
                     RKPSVPLLFQMMELLDLSVDALVFQSREESRCLRMDGLSEREAEALERLVDAMREK"
     gene            complement(3907..4872)
                     /locus_tag="ACZPAQ_02700"
     CDS             complement(3907..4872)
                     /locus_tag="ACZPAQ_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HIR0902920.1"
                     /translation="MSGTLLDLILYLALVALGAFLGSRPQIQSLPMPWLGRFQTAALL
                     LIVTLGAELGSNEEIISSLGTIGLNALAVTLTSMVGSMLAVHALRKYLLKLNAQGRSC
                     QEAPEDSDPGSGEAVHVDHSLTFAIVFTVAAGILAGRFLLPDAAVAHCGTVIRWGLYL
                     LLFLVGLDMGRQGSLIRDIRAAGLRVLLVPLAVMAGTFAVTALVGLFLPLGPKDCVAA
                     AAGFGWYSLAPTLLAPYSLTVSATAFLSNIMHELFSIVAVPIVAQKLGYIEAVALPGA
                     TAMDTLLPVVVSATADRMAIYSFASGLILSLAVPVLVPAIIALPL"
BASE COUNT         1135 a         1506 c         1777 g          936 t
ORIGIN      
        1 gccccgcttc tccattttga atccccgctt caccctcacc ctgccccggg agcagatggt
       61 cgcgggtatc tacgacatct tcaaccacat ctgcgagcag tacttctccg gggaggatga
      121 caacaccagc gactatctca gcgaggggct gatgcgctcc ctcctgtgct ccagccgggc
      181 tgccaaccgg gatccccagg actacgaggc caggagcaac atcatgtgga ccgccacctg
      241 ggcgctgaac accctggtgt cccggggcaa gtccaccgac tggatggtgc acatgctggg
      301 ccaggcggtg gggggctaca ccaacgccac ccacggcatg acgctggcgg cggtctccct
      361 accctactac cggcacatcc tcccctatgg gctggacaaa ttcaagcggt tcgccgtcca
      421 ggtgtgggga gtggacccgg cggaggaggg gctggcggcc atggagggct ggatgaagga
      481 gcttgggctg accatgaata ccagtgcgct gggcgtgacg gaggacatgc tggaggggct
      541 ggctgacgtg accatccacc tcaccggggg ctacaaggat ctggatcggg aggagatcct
      601 tcagatcttc cgggagagcc tgggctgacc agacagaaag ctctgcaaat aaggcgcagc
      661 gcccggagta ttctccaggc gctgcgcttt tttcaccatc ccttcccccg cggtccaggg
      721 gcggggagtg cgccctcgtc gggctggaat tccgtcatag tgccccagaa ccgctttggc
      781 atgttggtca tccggcaccg gggattttcc cgttcccgga tggcgatggt gtcatagaac
      841 cgcctccaca gacgccggta ggtcacctcc tcctgccccg gcggggcagg ggcaaagtct
      901 tccgcgggca gaatggccca ccgaccgggc tgatggatca gcagctcccg gtgggtgtgg
      961 tcgtagatga ggaagtgctc ctcagagtac cgggtgcaga agtgggggcg gaggagggga
     1021 agcacccggt ttttgggctc gatctcccct accagcaccc cctgctgatc ggagaagcgg
     1081 aggaacccct tgagcagatg ggcctcgctc cccaggtgct gcaccgcctt ccacagcacg
     1141 tggacccggt cgtcggtgag atcccgggtg atgtccgccc cccgttggta gcccagccgc
     1201 aggaaacggt acatgtgcag ctcccgctcg ggcagacagg tgagaaaagc ctgggtcacc
     1261 agccggcagg cctgggggga gatctttttg gacagagagc ggtacacccg ctccgccttg
     1321 gcctggtcgg tgtccacatg ccgctccggc cagagggtga ggcggtcctc ttccggtgag
     1381 gtgaagcaga cggcctcctc gcggtgggca tagctctcaa acacacaggt gagaaagccg
     1441 ggataactgc cgtcataatc gaagcagacc tgctgccagc tcatgaggcc ccctcctctc
     1501 caaagaggga gagctgctcc ggctggacct gggccagggc ggggcgatcc agggcagcca
     1561 gatgggacag gatgccatcc tcccgcactc gcagaccctc cagcatgcgg ccggagcagg
     1621 tgatgaagta ctgggcccgc ttgaggacca cccccagctt tttcaggccg gagaaggaca
     1681 ggggtccggc ccgccgggct gtgaggatgc gcttggcgct ggtgaccccg atgccgggca
     1741 cccggagcag ggcctcatag tcggcccgct ggacctccac aggaaactgg tccaggtggc
     1801 gcagggccca gccgcacttg gggtccagac ggaggtcgaa gtcgggccgc ccctcgtcca
     1861 gcagctcctc ggcccgaaag tggtagaagc gcagcagcca gtccgcctga tacagccggt
     1921 gttcacgcag caggggcggc tgaaagccct ggggagcggg gagcagggag ctggaggaga
     1981 cggggatata ggcggagtag aagacccgct tcagatggta cttgtcgtag agcccctggg
     2041 tgagcatcag gatgtggcgg tcgctctccg gcgtggcacc tacgatcatc tgagtggact
     2101 ggcccgccgg ggcgaagcgg ggggcatggt ggtatttggc cagctcctgc cgggactggg
     2161 tgatcccatc ccggatctgg gccatggggg ccaggatacc ggcccggccc ttgtccgggg
     2221 ccagcagggc caggcttttg gcgctgggca gctcgatgtt gacgctgagc cggtcggcca
     2281 gcaggcccag ctgctgggtg aggaggggat ctgcacctgg gatggccttg gcgtggatat
     2341 agccgccgaa gccataatcc tggcggagca ggcgcagtgc ctcgatcatc cgctcggtgg
     2401 tataatccgg gtcccgcagc acggcggagg agagaaagag cccctcaata tagttgcggc
     2461 ggtagaactg gatggtgagt tcggccagct cccggggagt aaaagcagcc cggggcggat
     2521 cgttggaccg gcggttgaca cagtactggc agtcgtaggc gcagatgttc gtatagagga
     2581 tcttcaggag ggagatgcac cgcccgtcag cggagaagga gtggcagcag cccgccgcca
     2641 tggtggagcc cagccggccc gcccgtccgg cccggtccag tccgctggag gtgcaggcgg
     2701 cgtcatactt ggcggcgtcc gccaggatgg tcagcttgtc catcaggtcc atggggtctc
     2761 cctcccttga aatcgaacat atgtattata atagaccaaa catatgtttc tgtcaagggg
     2821 agagaacatg ccaaaacctt tccaatgggg gaaggtgata cggtgcccat attggaggag
     2881 cgcggggccc gatctgcgga tcgggccccg cgccctttta gtgctctgga gtgccatgga
     2941 gaggggagaa gtccgcacgg agacaccggg ggccggcgga agtgacctcc ggtgcgcccc
     3001 ggtaatacac ctgaagagcg gcgaggagaa aatgagtcag ttgggccgcc tccgatccgc
     3061 tgaggggcag cgcacccggg ggcaggaggg gcaggcgcct gcggatctgg aggcgtccca
     3121 agtttccagg agaaaagaga ccagcccgga gcaagaggtc acagtccttg atctcgatcc
     3181 agcacaggtc cgtcgtgccg gcgtccagcg cactcccggc ggcccagtcc cgccggttat
     3241 ccaggaagac gatggtctcc tccagggcct ggcggaatgc cgggggtatc tctgagtttc
     3301 catgaaaaaa gtctcgggtc agcggcccat gagccgccct cctgtgggcg atataagagg
     3361 cactgtgaga aaggacccat cctcccaccc ctaatatcat caaaagcaga atgacggact
     3421 tcatcataga cggcggcctc ttcgtctcag gtatcttttt tggttatctt atagatacta
     3481 tgctagcact cttttagata attcgcaagg gaaaagatta tctgtaagat aacggagagg
     3541 gagttgtcag aaatgagcat ggaaacaagg gccctggggg atgccatccg cgcggcccgc
     3601 atgaagaagg ggctgactca ggatgcgctt tcggagcttc tggatatcac gcccactcat
     3661 ttgaagaata tcgagggctc ccgtcggaag ccctcggtgc ctcttctgtt ccagatgatg
     3721 gagcttctgg atctctccgt ggatgctctg gtgttccaga gcagggagga gagcaggtgt
     3781 ctgcggatgg acggcctttc ggagcgggag gcggaggcgc tggagcgtct ggtggacgcc
     3841 atgcgggaaa aatagagcac cggcttgggc gtgggcctga gccggtgctc ttgttctgga
     3901 aagtgttcag agggggagcg cgatgatggc ggggaccagc actgggacgg ctagggagag
     3961 aatcagtccc gaggcaaagg agtagatggc catgcggtcg gcagtggcgc tgactaccac
     4021 cggtagcagg gtatccatag ctgtggcacc ggggagggcc accgcctcga tatagcccag
     4081 cttctgggcc acgatgggga cggccacaat ggaaaacagc tcgtgcatga tgttggacag
     4141 gaaagcggtg gcagatacgg tgagggagta cggagccagc agggtggggg ccagggaata
     4201 ccagccgaat ccggccgctg ccgctacgca gtccttgggg cccaggggca ggaacagccc
     4261 taccagggcc gtcaccgcaa aggtgcccgc cataaccgcc agcgggacca gaaggacccg
     4321 gagcccggcg gcccgaatat cccggatcag gctgccctgc cggcccatat ccaggcccac
     4381 cagaaagagg agcaggtaca gcccccagcg gatgaccgtg ccacagtggg ctacggcggc
     4441 gtcggggagc aggaagcggc ccgccagaat gcccgccgct acggtgaaga cgatggcgaa
     4501 ggtcaggctg tggtccacgt ggaccgcctc cccggaaccc gggtcgctgt cctccggtgc
     4561 ctcctggcag gaacggccct gagcgttcag cttcagcaga tattttctca gggcgtggac
     4621 agccagcatg ctgcccacca tggaggtgag ggtgaccgcc agggcgttga gcccaatggt
     4681 gcccagggaa gagatgatct cctcgttgga ccccagctcg gcccccaggg tcacgatcag
     4741 cagcagagcc gccgtctgaa accggcccag ccagggcatg ggaagggact ggatctgggg
     4801 gcgggagccc agaaaggccc caagagccac cagagccaga tagaggatca gatcaagaag
     4861 ggtgccggac atctgctgca acgggatcac tcctgacatg aatagacgaa aagaaacgga
     4921 gggcgggtct cctgccctcc ggacgcggac aaaaaattat tatagcatgg gtgaagaaaa
     4981 aagtccagaa aaatggggaa aggtccctgt tcaagctaag tgctgatgat tccaactccg
     5041 gcgatttcac cagtgtgcct tgcacaaaac atgtttatat tttataatga tagaaaatga
     5101 aacaccaaag aatttttgct atcatcataa ataaagtagt tagacaaatc tgataaagga
     5161 acgtgattgt atggattggt ataaaaatat gaccaaacag caaagggaag atttttggaa
     5221 agttataact gtaagtgtaa caggagcaat tatagtttta cttgttccta caatttttca
     5281 tatagtttga agctaaaaat cccattttga ccgttattta ctccgttgaa aagccaaaca
     5341 aggcaaaaat tatc
//
LOCUS       DBMDSJ010000070         5350 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4310_length_5350_cov_1.573062, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000070 DBMDSJ010000000
VERSION     DBMDSJ010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4310_length_5350_cov_1.573062"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="ACZPAQ_02705"
     CDS             complement(<1..456)
                     /locus_tag="ACZPAQ_02705"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR0902921.1"
                     /translation="MISIIKADGTSEVRQLEAMRARAAQKNADIELAVSAVMQNVKEN
                     GLSAVRDYSRQFDHREPYELTRAQLEEAYRSCPAELIAALEHAAANIRDYNEKLLSRT
                     MEWTSPDGGRVGRIVRGLSRVGIYVPGGTAAYPSSVLMNAVPAKVAGAEE"
     gene            complement(453..1094)
                     /gene="hisG"
                     /locus_tag="ACZPAQ_02710"
     CDS             complement(453..1094)
                     /gene="hisG"
                     /locus_tag="ACZPAQ_02710"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR0902922.1"
                     /translation="MSGRLRIALTKGRLQDKSVELFERMGLDCTPVRDPGRRLVHAIP
                     NYPLDAVLAKAPDVITYVEHGVCDLGVVGKDTILEKGGAFYEVLDLGFGRCRFALAVK
                     EGTDFYGTYKTRRVASKYPAVTRAFFAEKGMDVDIIKIEGSVELAPILSLTDAIVDIV
                     ETGATLKANGLVPIETVAPVSARLIVNTASMKLHKSQINDFIDRCEAELERDI"
     gene            complement(1091..2260)
                     /gene="hisZ"
                     /locus_tag="ACZPAQ_02715"
     CDS             complement(1091..2260)
                     /gene="hisZ"
                     /locus_tag="ACZPAQ_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR0902923.1"
                     /translation="MKLTVNTPEGTRDRLFAECMGRRQVQERLIRLFCQRGYREVSTP
                     ETEFYDLFARSGSAIPQEQMVQVFDQSGKLCVMRPDSTTPIARVAATKLRALPLPQRL
                     YYDQTVYRAGPAHSGGNRELPQCGVELIGAAGMKADLEMVATAVDALRVCGASRFHVE
                     LGHAGFFRDVAARMELEEGEMERMRALIEGKNFAALRDMLAPFRENPASAALLRLSRL
                     FGGPEVLDEAEALAGETEAVEYLRALYGELSAAGYGALVRFDLGMVHQIDYYTGVVFR
                     GYVEGAGDAVLSGGRYDSLVGVFGREAQATGFAVDVDAMARTLPEAAPSALDTVVHYA
                     PGYLAKALEAVDSRPAGTCELSPCRTLESSCSLAREKGAKTVLVFDVTGERVVKV"
     gene            complement(2429..2941)
                     /locus_tag="ACZPAQ_02720"
     CDS             complement(2429..2941)
                     /locus_tag="ACZPAQ_02720"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR0902924.1"
                     /translation="MLDAKVAQLLNEQVNKEFYSAYLYLDFSIYYEEEGLDGFANWYK
                     IQAQEERDHAMLMVQYLQNNGGKVTLEAIAKPDRELTSRLRVLELGLEHERYVTGLIH
                     TLYEAAYSAKDFRTMQFLDWFVKEQGEEEKNAEDLIKKMKLYGEDAKGLYMLNSEMAA
                     RVYTAPSLVL"
     gene            complement(3133..>5350)
                     /locus_tag="ACZPAQ_02725"
     CDS             complement(3133..>5350)
                     /locus_tag="ACZPAQ_02725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HIR0902925.1"
                     /translation="KDEQQLQEFNRTIVRLGEKLNIPVCATCDVHFMDPEDEMYRRIL
                     MAGQGFTDADNQAPLYLRTTGEMLAEFQYLGKEKAYEVVVTNTNAIADRVENIRPIPL
                     GTFPPHIDGADQQLQDITWGRAKEIYGDPVPEIVASRLDRELTSIIKHGFAVLYMIAQ
                     KLVKNSVDNGYLVGSRGSVGSSLVAFMSGITEVNSLPAHYRCPKCKHSDFDYAQDPAH
                     PYGCGADMPDMNCPVCGTPYVKDGFNIPFETFLGFGGDKVPDIDLNFSGEYQSSAHRY
                     TFELFGQTHVFRAGTIGTVAEKTAFGYVKKYLEERGRTASKAEENRLAIGCTGVKRTT
                     GQHPGGMVVIPQDKEIYDFCPVQHPADDPNTDIVTTHFEYHSMESNLLKLDMLGHDDP
                     TMIRMLQDLTGVDPQKIPLDDPETMSIFQSSKVLGYENDKILGPTGGTAIPEFGTSFV
                     RGMLEDTQPNQFDILVRLSGFSHGTDVWLGNAKDLITSGTAKVSEAIGCRDDIMLFLI
                     SKGMDPKRSFKIMEAVRKGRGLPEGAEEEMKEHGVPDWYIGSCKKIAYLFPKAHAVAY
                     VMMAFRIAWFKVHRPLAFYAAYFSIRAKAFDQEFMCQGMDVCQKKMREIIAKDKDASA
                     VEQDMLTTLEVCYEFYLRGFTFRRMDLYLSDALHFRVDEEHKALIPPFISVAGLGETA
                     ALSLAEQAKGRRFISVEEVSAACPKVSKTHIDKLTEAGAFGDMPATSQLDLFAMLG"
BASE COUNT          944 a         1785 c         1584 g         1037 t
ORIGIN      
        1 ctcctccgcc cccgccacct tggcggggac ggcgttcatc agcacgctgc tgggataggc
       61 ggcggtcccg cctgggacgt agatgcccac ccggctcagc ccccggacaa tccggcccac
      121 ccggcctccg tccggtgagg tccactccat ggttcgggag agcagcttct cgttgtagtc
      181 ccggatattg gccgcggcgt gctccagggc ggcgatcagc tcggccggac agctgcggta
      241 ggcctcctcc agctgcgccc tggtgagctc atagggctcc ctatggtcaa actgccggga
      301 gtagtcccgg acagccgaaa ggccattttc ctttacattc tgcatgactg ctgagacagc
      361 cagctcgata tctgcgtttt tctgtgcggc gcgggcgcgc atggcctcca gctgccgcac
      421 ctcggaagtg ccatccgctt tgatgatgga aatcatatgt ctctctccaa ttccgcctca
      481 cagcggtcga taaaatcgtt gatctggctc ttgtgcagct tcatgctggc ggtgttgacg
      541 atcagccgtg cggacaccgg ggccactgtc tcgatgggca ccaggccgtt ggccttcagg
      601 gtagccccgg tctccacgat atccacaatg gcatccgtca gagagaggat gggggccagc
      661 tccacgctgc cctcgatctt gatgatgtcc acatccatcc ccttctccgc gaaaaaggcc
      721 cgggtcaccg ccggatactt ggaggccact cgccgggtct tataggtccc atagaagtcg
      781 gtcccctcct tcactgccag agcgaagcgg cagcggccga agcccaggtc cagcacctca
      841 tagaaggcgc cccccttctc caggatggta tccttgccca ccacccccag gtcacatacc
      901 ccgtgctcca cataggtgat gacgtcgggc gccttggcca gcacagcgtc caggggatag
      961 ttggggatgg cgtggaccag ccgccgcccc gggtcccgga cgggggtgca gtccagcccc
     1021 atcctctcaa acagctccac cgacttgtcc tggagccgcc ccttggtcag ggcgatacgc
     1081 agacggccgc tcataccttc accacccttt ctccggtcac atcaaagacc agcacagtct
     1141 tggcgccctt ctccctggcc aggctgcagc tggattccag cgtgcggcag ggggagagct
     1201 cacaggtccc cgccggccgg ctgtccaccg cctccagcgc cttggccaag tagccggggg
     1261 cgtagtggac cacagtgtcc agcgcggatg gggcagcctc gggcagggtg cgggccatag
     1321 cgtccacatc tacggcaaag ccggtggcct gggcctcccg gccgaatacc cccaccagcg
     1381 agtcgtagcg tccgccagac agcaccgcat cccctgctcc ctccacatag ccccggaaga
     1441 ccacgccagt atagtagtcg atctggtgga ccatccccag gtcaaagcgg accagcgcgc
     1501 cgtatcccgc cgcggacagt tccccgtaca gggctcggag gtactccacc gcctccgtct
     1561 ccccggccag cgcctccgcc tcatccagca cctccggccc gccgaacagc cgggagaggc
     1621 gcagcagggc cgcgctggcg gggttctccc ggaagggggc gagcatatcc cgcagggcgg
     1681 cgaagttttt gccctcgatc agggcccgca tccgctccat ctccccctcc tccagctcca
     1741 tccgggcggc cacatcccgg aagaagccgg cgtggcccag ctccacatgg aaccgtgagg
     1801 ccccgcacac ccgcagggcg tccactgctg tggccaccat ctccagatcc gctttcatcc
     1861 cggcggctcc gatcagctcc accccgcact gggggagctc ccggttcccg ccgctgtggg
     1921 ccggcccggc ccggtacacg gtctggtcgt agtacagccg ctggggcagg ggcagggccc
     1981 gaagctttgt ggccgccacc cgggcgatgg gggtggtgga atccggccgc atgacgcaga
     2041 gcttcccact ttggtcgaac acctgtacca tttgttcctg ggggatggca ctgcccgagc
     2101 gggcaaacag gtcgtaaaac tccgtctccg gggtggacac ctcccgatag ccccgctggc
     2161 agaagagacg gatcagccgc tcctgcacct gtcggcggcc catgcactcg gcaaacagac
     2221 ggtcgcgggt gccctccggg gtattgacag tcaatttcat gatctggctc cttgtcaacc
     2281 gcgggacagc gcgctgtctc cgcctccatt ctttactacg ctaatatact actataagaa
     2341 agtgctgtca agaaaaatct tgcacccagg aaaaagcggc gcggagcagg acccgttccg
     2401 gtcctgctcc gcgcaaagca aattggggtc acagcacgag agagggggcg gtgtagaccc
     2461 gggcggccat ctcgctgttg agcatataca gccccttggc gtcctcgccg tagagcttca
     2521 tctttttgat caggtcctcg gcgttcttct cctcctcccc ctgctccttg acgaaccagt
     2581 ccaggaactg catcgtccga aaatccttgg cgctgtaagc ggcttcatac agcgtgtgga
     2641 tcaggccggt gacataccgc tcgtgctcca gccccagctc cagcacccgc aggcggctgg
     2701 tcagctccct gtcgggcttg gcgatggcct ccagcgtcac ctttccgccg ttgttctgga
     2761 gatactgcac catcagcatg gcgtgatccc gctcttcctg cgcctggatc ttgtaccagt
     2821 tggcaaagcc atccaggccc tcctcctcat agtagatgga gaaatccagg tacagatagg
     2881 cggaataaaa ctccttgttg acctgctcgt tgagcagctg cgcgaccttt gcgtccagca
     2941 taaaaaacaa ctcctttttc ttttttctga aactctttct gagtcaagat cctccccctc
     3001 tgttccccgc tctggtgcgg gacagacaga cggatcagga cctcgatctc tccacgatct
     3061 gcctctatcc taacacagaa ccgggcatcc ggcaacctcg atcccatcca tctgtgcaga
     3121 ccgttccatc ggtcacccca gcatggcgaa caggtccagc tggctggtgg ccggcatatc
     3181 tccgaaagcc cccgcctccg tcagcttgtc gatgtgggtc ttggagacct tggggcaggc
     3241 agcggagacc tcttccacag agatgaacct ccgtccctta gcctgctccg ccagggacag
     3301 ggccgccgtc tcccccagcc ccgccaccga gatgaagggc gggatcagcg ccttgtgttc
     3361 ctcgtccacc cggaagtgga gggcgtccga caggtacagg tccatccttc ggaaggtgaa
     3421 tccacgcaga tagaactcat agcacacctc caaggtggtg agcatgtcct gctccaccgc
     3481 ggaggcgtcc ttgtccttgg cgatgatctc cctcatcttc ttctggcaca catccatccc
     3541 ctggcacatg aactcctggt caaatgcctt ggcccggatg gagaagtagg cggcgtaaaa
     3601 ggccagaggc cggtggacct tgaaccaggc gatgcggaag gccatcatca cataggccac
     3661 cgcatgggcc ttggggaaca gataggcaat cttcttgcag gagccgatgt accagtcagg
     3721 caccccgtgc tccttcatct cctcctccgc cccctccggc aggccgcggc ccttccgcac
     3781 cgcctccatg atcttgaagg agcgcttggg gtccatcccc ttggagatca ggaacagcat
     3841 gatgtcgtcc cggcagccga tggcctcgct gaccttggcg gtgcccgagg tgatcaggtc
     3901 cttggcgttg cccagccaca cgtcggttcc gtgggagaag ccggacaggc ggaccaggat
     3961 atcgaactgg ttgggctggg tgtcctccag catcccccgg acgaaggagg tgccgaactc
     4021 cgggatggca gtgccgccgg tgggccccag gatcttgtcg ttctcatagc ccagcacctt
     4081 ggaggactgg aagatagaca tggtctccgg gtcatccagt gggatcttct gggggtccac
     4141 cccagtcagg tcctgaagca ttcggatcat agtggggtca tcgtgtccca gcatgtccag
     4201 cttcagcagg ttggactcca tggagtgata ttcgaagtgg gtggtgacga tatcggtatt
     4261 ggggtcgtcc gccgggtgct gtacgggaca gaagtcatag atctccttgt cctgggggat
     4321 gaccaccatg ccgccggggt gctggccggt ggtgcgcttg acgccggtgc agccgatggc
     4381 caggcggttc tcctccgcct tggaggccgt cctcccccgc tcctccaggt acttcttcac
     4441 atagccgaaa gccgtctttt ccgccaccgt accaatggtc ccggcccgga acacgtgggt
     4501 ctgtccaaac agctcaaagg tgtaccggtg ggcgctggac tggtactcgc cggagaagtt
     4561 caggtcgata tcgggcacct tgtctccccc gaagcccaga aaggtctcga aggggatgtt
     4621 gaagccgtcc ttcacatagg gcgtgccgca gacgggacag ttcatatcgg gcatatccgc
     4681 cccgcacccg taggggtggg cggggtcctg ggcgtagtca aagtccgagt gcttgcactt
     4741 ggggcagcgg tagtgggcgg gcagggagtt gacctcagtg atgcccgaca tgaatgccac
     4801 cagagaggag cccaccgatc cacgggagcc caccagatag ccgttgtcca ccgagttttt
     4861 caccagtttc tgggcaatca tgtacagcac ggcgaacccg tgcttgatga tggaggtcag
     4921 ttccctgtcc agacggctgg ccacgatttc cggcaccgga tcgccgtaaa tctccttggc
     4981 ccggccccag gtgatgtcct gcagctgctg atccgccccg tcgatatggg gcgggaaggt
     5041 gcccaggggg atcgggcgga tgttttccac ccggtcggca atggcgttgg tgttggttac
     5101 caccacctca taggccttct ccttgccgag atactggaac tccgccagca tctcgccggt
     5161 ggtgcgcagg tacagcggcg cctggttgtc cgcgtcggtg aacccctgcc ccgccataag
     5221 gatccggcga tacatctcgt cctccggatc catgaagtgg acgtcgcagg tggcgcacac
     5281 cgggatgttc agcttctcgc ccaggcggac gatggtgcgg ttgaactcct gcagctgctg
     5341 ctcatccttg
//
LOCUS       DBMDSJ010000071         5343 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4317_length_5343_cov_1.567531, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000071 DBMDSJ010000000
VERSION     DBMDSJ010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4317_length_5343_cov_1.567531"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="ACZPAQ_02730"
     CDS             complement(<1..475)
                     /locus_tag="ACZPAQ_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HIR0902926.1"
                     /translation="MGKTVIPEGYRSLLSLYETQKAISLLKRLFEDRLAGALRLHRVS
                     APLFVSASSGLNDDLNGVERPVSFEINRVEDTAQVVHSLAKWKRLALKRYQFHPGEGL
                     YTDMNAIRRDEELDNLHSAYVDQWDWEKIITQEDRTRDYLKQTVNAIVRALVDTQS"
     gene            674..1432
                     /locus_tag="ACZPAQ_02735"
     CDS             674..1432
                     /locus_tag="ACZPAQ_02735"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR0902927.1"
                     /translation="MKFVSWNVNGLRACLKKGFLDFYRQEAPDFCCLQETKMEQGQAD
                     VGLNEGVLEFWNSAEKKGYSGTAVFTPHQPVAVAYGMDKDRHDHEGRLITLEYEGFYL
                     VCCYTPNSQDGLKRLDYRMEWEDDLRDYLMSLDRHKPVVYCGDLNVAHREIDLKNPKT
                     NRNNAGFTDQEREKMTCLLSSGFTDSFRYLYPDTEGIYSWWSYRFNARKNNAGWRIDY
                     FIVSDRLRDKIKRAEILTTVEGSDHCPVLLELDI"
     gene            1476..1949
                     /locus_tag="ACZPAQ_02740"
     CDS             1476..1949
                     /locus_tag="ACZPAQ_02740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR0902928.1"
                     /translation="MRRAEKEVKDLEQLEEILRDCNTVRIGTQDAEGMFIVPVNYGYD
                     LTEGQLTLYLHSAREGRKAAAFRRGGAVAFEMDCGHQLMRAEQACSYSCAYRSIMGSG
                     TIRELTEPQEKYEALNAIMRHMTGRNWEMPEPAVARVSVFAIRAERWTGKRSPAN"
     gene            1965..3593
                     /locus_tag="ACZPAQ_02745"
     CDS             1965..3593
                     /locus_tag="ACZPAQ_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR0902929.1"
                     /translation="MVLFDPHRYAYPSRRSVVYARRAMACTSVPLGAQVGLDVMKAGG
                     NAVDAAVAMAAAMPLLEPTGNGIGGDCFALVWVEREKKLYGLNASGVSPAALSAALVR
                     ERGFGEMPKEGWLPTMVPGAPGGWAELNRRFGTRPLEELFAPAASYAREGVAVPVNVA
                     VQWALDGPRIGRAMERDPAPHRPWWERMMKADGTPYRAGEIFRWPEYADTLEELAATS
                     CESFYRGSLMERMVSFSRETGGFFQERDLQDYRPEWVEPITADYKGYTVCEIPPNGHG
                     ITVLMALNILKGLPLSQERDSADTYHKILEAIKLAFADTRTYVADPRYMRTRVADLLS
                     EDYAARRRALIGERALLPQAGDPSCGGTIYLCTADPQGNMVSFIQSNYTTFGAGIRIP
                     GTGICLQNRGANFSLDEGSDNCLAGGKKSYHTIIPGFLMKEGTAVGPFGVMGAFMQPQ
                     GHVQVVVNTVDYHMNPQECLDAPRMQWTGGKRIQLEREVPAHIGLRLAEMGHQVEICN
                     SNLDMGRGQIIWRMEDGTLAGGTEPRCDGTVAAW"
     gene            3612..4127
                     /locus_tag="ACZPAQ_02750"
     CDS             3612..4127
                     /locus_tag="ACZPAQ_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902930.1"
                     /translation="MKEHGLIRIIILLSGALLWVAYLFDAVCSRYAIYEVIGYGAHEL
                     LSMLPYVSALATAVWLAITLVKGARGKTLRRELAFCGVLAALLAVQWGYLIERSHRST
                     ATVLAQVTETVLVPAQGLQAVIRHPEGYEITLWCPQLAWELLQPGTEYLVTYEWDDRM
                     PQEGTLCMVHP"
     gene            4160..>4447
                     /locus_tag="ACZPAQ_02755"
     CDS             4160..>4447
                     /locus_tag="ACZPAQ_02755"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008717729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR0902931.1"
                     /translation="MKIAELFGKGKTVFSCEVFPPKKDSPVDTIYQTLDGLKDIKPDF
                     ISVTFGAGGSQVNQSTREIAAIIQKQYHIPAMAHLTCITATRGEVTELLYDL"
     assembly_gap    4448..4457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4458..5034
                     /locus_tag="ACZPAQ_02760"
     CDS             <4458..5034
                     /locus_tag="ACZPAQ_02760"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR0902932.1"
                     /translation="ADGVENVLALRGDRNPDYPPKTEFLHADELVAFIRRRGDFGVSA
                     ACYPEGHPESPDAVSDLRYLKQKVDAGAQHLVSQLFFDNEDFLRFLERARIAGITVPI
                     EAGIMPVLNQKSIERMVSLCGASIPRKLSRILARYGDHPEALREAGIAYAIDQISDLV
                     AAGVDGIHLYTMNNPAVARQISDSVSAIRRV"
     gene            5040..>5343
                     /locus_tag="ACZPAQ_02765"
     CDS             5040..>5343
                     /locus_tag="ACZPAQ_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902933.1"
                     /translation="MPIALGELDLDEVLRYMGTPPSQAGPELRALAEECGRAVCAAAR
                     PRWAWRAFDIAEEADGVRLSCGLLLPGQDLKRHLRGCGRAAVFCATLSAQVDALIRR"
BASE COUNT          983 a         1628 c         1821 g          901 t
ORIGIN      
        1 aggactgggt atccacgaga gcgcggacaa tggcgttcac cgtctgcttc agatagtccc
       61 gggtgcggtc ctcctgggtg atgatcttct cccaatccca ctggtccacg taagcggagt
      121 ggaggttgtc cagctcctca tcccggcgga tggcgttcat gtcggtgtag agcccctctc
      181 cggggtggaa ctgatagcgc ttcagagcca ggcgcttcca cttggccagg gagtggacca
      241 cctgggcggt gtcctccacc cggttgatct caaaggagac ggggcgctcc accccgttca
      301 ggtcatcgtt gaggccggag gaggcggaga cgaacagggg ggcagacacc cggtgcaggc
      361 gcagggcacc ggcgaggcgg tcctcgaaca gacgcttgag caggctgatg gccttctggg
      421 tctcatagag gctgaggagg gagcggtatc cctcgggaat gacggttttt cccatgtcga
      481 atactccttt tgaaagggat ttggaacaga agctcatccg gcagagccgg gatggaacgc
      541 ctggtgagca catacagatt tttctattat agcggagaag ggcggaaaaa tcaatctgtt
      601 cttgccaagc ggggcacgtc tttgtataat agggtccatc caaagttccg acggagggag
      661 agaggagatg cctatgaaat tcgtttcctg gaatgtgaac ggcctgcggg cctgcctgaa
      721 aaaggggttt ctggactttt accgacagga ggcacccgac ttctgctgtc ttcaggagac
      781 caagatggag cagggacagg cggatgtggg gctgaacgaa ggggtcttgg agttctggaa
      841 ctctgcggag aagaagggat actccggcac cgccgttttc accccccacc agcctgtggc
      901 tgtggcctac ggtatggaca aggaccggca cgaccacgag gggcggctca tcaccctgga
      961 gtatgagggc ttctatctgg tctgctgcta cacccccaac tcccaggacg gcctgaagcg
     1021 gctggactac cgcatggagt gggaggacga cctgcgggac tacctcatgt ccctggaccg
     1081 gcacaagccg gtggtctact gtggggacct gaacgtggcc caccgggaga tcgacctgaa
     1141 aaacccaaag accaaccgga acaacgccgg cttcaccgac caggagcggg aaaagatgac
     1201 ctgcctgctc tcctccggct ttacagacag cttccgctat ctctacccgg acacggaggg
     1261 gatctactcc tggtggtcct accgcttcaa cgcccggaag aacaacgcgg ggtggcgcat
     1321 cgactatttc atcgtctctg accgcctccg ggacaagatc aagcgggcgg agatcctgac
     1381 cacggtggag ggcagcgacc actgcccggt actgctggag ctggacatct gagtgccccg
     1441 gggcgggacc ggaaggagat aaggaggatc tggatatgcg cagagcggag aaggaagtca
     1501 aagacctgga gcagttggag gagatcctgc gggactgcaa cacagtgcgg atcgggaccc
     1561 aggatgcgga ggggatgttc atcgtccctg tgaattacgg ctacgacctg acagaggggc
     1621 agctgaccct ctacctccac tcggcccggg aggggcggaa ggcggcggcg ttccgccggg
     1681 gcggggcggt ggcctttgag atggactgcg gccaccagct gatgagggcg gagcaggcgt
     1741 gcagttacag ctgtgcctac cgcagcatca tgggcagcgg gaccatccgg gagctgaccg
     1801 agccgcagga gaaatatgag gccctcaacg ccattatgcg ccatatgacc gggcgaaact
     1861 gggagatgcc ggagcccgcc gtggcccggg tatccgtctt tgccatccgg gcggagcgct
     1921 ggacgggcaa gcgcagccca gccaactgag aggaggcagc cgaaatggtc ttatttgatc
     1981 cccacagata cgcctatccc tcccgccgca gcgtggtgta cgcccgccgg gccatggcct
     2041 gtacctccgt ccccctgggg gcccaggtcg gcctggatgt gatgaaggcc gggggaaatg
     2101 ccgtggatgc ggcggtggcc atggccgccg ccatgcccct gctggagccc acaggaaacg
     2161 gcatcggggg ggactgcttc gccctggttt gggtggagcg ggagaaaaag ctgtacggcc
     2221 tgaacgccag cggcgtgtcc cctgcagctc tgtccgccgc cctggtgcgg gagcggggct
     2281 tcggggagat gcctaaagag ggctggctgc ccaccatggt gcccggcgcc cccgggggat
     2341 gggcagagct caaccggcgc ttcgggaccc gcccccttga agagctgttc gcccccgcgg
     2401 cctcctacgc cagggagggg gtggctgtgc cggtgaacgt ggcggtccag tgggcactgg
     2461 acggcccccg tatcggccgg gctatggagc gggacccggc cccccaccgc ccctggtggg
     2521 agcggatgat gaaggcggac ggcaccccat accgggcggg agagatcttc cgctggccgg
     2581 agtacgctga cactctggag gagctggcag ccaccagctg tgagagcttc taccggggga
     2641 gcctgatgga gcggatggtc tccttcagcc gggagacggg gggctttttc caggagcggg
     2701 atctccagga ctaccgcccg gagtgggtgg agcccatcac cgcggactac aagggctaca
     2761 ccgtgtgcga gatcccgccc aacggccatg ggatcaccgt gctgatggcg ctgaatatcc
     2821 tgaagggcct gcccctctcc caggagcggg acagcgccga cacctaccat aagattttgg
     2881 aggccatcaa gctggctttc gcggacacca ggacctatgt ggcggacccc cggtacatgc
     2941 ggacccgggt ggccgacctg ctctcggagg actatgctgc ccggcggcgg gccctgatcg
     3001 gagagcgggc cctgctgcca caggcggggg acccctcctg cggcggcacc atctacctgt
     3061 gcaccgcgga cccccagggc aacatggtct ccttcatcca gagcaactac accaccttcg
     3121 gtgcgggcat ccgcatcccg ggcaccggta tctgcctcca gaaccggggg gccaacttct
     3181 ccctggatga gggcagcgat aactgcctgg ccggcggcaa gaagtcttac cacaccatca
     3241 tccccggctt cctgatgaag gaagggacgg cggtgggtcc tttcggcgtg atgggggcct
     3301 tcatgcagcc ccagggccac gtgcaggtgg tggtgaacac ggtggattac catatgaacc
     3361 cccaggagtg cctggacgct ccccggatgc agtggaccgg tgggaagcgc atccagctgg
     3421 agcgggaggt gcccgcccac atcggcctgc gcctggcgga gatgggccac caggtggaga
     3481 tctgcaactc caacctggac atgggccggg ggcagatcat ctggcggatg gaggacggca
     3541 ccctggccgg cggcaccgag ccccggtgcg acggcacggt agcggcctgg tgaagagaga
     3601 ggagagatct tgtgaaagag cacggcctga tccggatcat tattctcctc tccggcgccc
     3661 tgctgtgggt ggcctacctg tttgatgcgg tctgttcccg atacgctatc tatgaggtga
     3721 tcggctatgg ggcccatgag ctgttgtcta tgctccccta tgtgtctgcc ctggccaccg
     3781 cggtctggct ggccattacg ctggtgaaag gggcccgggg aaagacactg cgccgggagc
     3841 tggcgttctg tggggtcctg gctgccctgc tggcggtgca gtggggctat ctgatcgagc
     3901 gatcccaccg ctccaccgcc accgttctgg cccaggtcac agagacggtc cttgtgccgg
     3961 cgcaggggct gcaggcggtg atccggcatc cggaggggta tgagatcaca ctgtggtgcc
     4021 cccagctggc atgggagctc cttcagccgg gaacggagta cctggtcacc tacgagtggg
     4081 atgaccgaat gccccaggag gggaccttgt gtatggtcca tccctgaaaa tctcaatttt
     4141 gatcaaggag gcagcagcca tgaaaatagc agaattgttc ggaaagggga agaccgtctt
     4201 ttcctgtgag gtgtttcccc ccaagaagga cagccccgtg gacactatct atcagaccct
     4261 ggacgggctg aaggatatta aaccagactt tatcagcgtt accttcggag ccggaggctc
     4321 ccaggtgaac cagtccaccc gggagatcgc ggccatcatc cagaagcagt accacatccc
     4381 cgccatggcc cacctgacct gcatcaccgc caccaggggg gaggtgaccg agctgctcta
     4441 tgacctgnnn nnnnnnnggc agacggcgta gagaacgtgc tggccctgcg gggggaccgg
     4501 aacccggact atcccccaaa gacggagttc ctccacgccg acgagctggt ggccttcatc
     4561 cgccggcggg gggacttcgg tgtgtccgcc gcctgttacc ctgaggggca cccggagagc
     4621 cccgacgcgg taagcgacct gcgctacctc aagcagaagg tggacgccgg cgcccagcat
     4681 ctggtgagtc agctcttctt tgataacgag gacttcctcc gcttcctgga gcgggcccgc
     4741 atcgcgggca tcacggtgcc catcgaggcg ggcatcatgc ccgtgctgaa ccagaagtcc
     4801 atcgagcgga tggtatccct gtgcggggcc tccatccccc ggaagctctc ccgcatcctg
     4861 gctcggtacg gcgaccaccc ggaggccctg cgggaggcgg gcatcgccta tgccatcgac
     4921 cagatctccg acctggtggc cgccggggtg gacggcatcc acctttacac catgaacaac
     4981 cctgcggtgg ccaggcagat cagcgacagc gtctccgcca tccggcgggt gtgagcgcca
     5041 tgccaatcgc cctgggggag ctggacctgg acgaggtgct ccgctacatg gggaccccgc
     5101 ccagccaggc cgggccggaa ctgcgggccc tggcggagga gtgcgggcgg gccgtatgcg
     5161 ccgccgcccg gccccgctgg gcctggaggg cctttgacat cgcagaggag gcggacgggg
     5221 tgcgcctgtc ctgcggcctg ctgctgccgg ggcaggacct gaagcgccac ctgagggggt
     5281 gcggacgggc ggcggtgttc tgcgccaccc tgtccgccca ggtggacgcc ctgatccgcc
     5341 gct
//
LOCUS       DBMDSJ010000072         5274 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4377_length_5274_cov_1.698434, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000072 DBMDSJ010000000
VERSION     DBMDSJ010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4377_length_5274_cov_1.698434"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="ACZPAQ_02770"
     CDS             <1..299
                     /locus_tag="ACZPAQ_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0902934.1"
                     /translation="DASGRRSPVPIQGSEFTMEADAVIMALGTSPNPMSYGGTPGLER
                     TRKGCVAADEAGHTSVEGVFAGGDAATGAATVILAMGAGKHAAKAIDEYLKSKK"
     gene            395..1294
                     /locus_tag="ACZPAQ_02775"
     CDS             395..1294
                     /locus_tag="ACZPAQ_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017209291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0902935.1"
                     /translation="MLYLRRFSLPGQGAEASFLARDSENKRTCYGSRYPFGIFLSRKV
                     PPLTFEPITILCGGNGCGKTTVLNLMAEKLKLRRGTVFNRSSFFQPYLDLCEAETAGA
                     FGAAELENSRIITSDDVFDGLLDLRYMNENVEHRRKELLEEYADARYARFQMRSLEDY
                     DRLRQVVEAQRKTGSHYVRDRVMKDIPGRSNGESAFLYFTREIQEGGLYLLDEPENSL
                     SAQLQLELARFLEDSARFYRCQFVISTHSPFLLALRGAKIYDLDQDPVEPCRWTQVPS
                     VRTFYEFFRSRREEFEDENGAPD"
     gene            complement(1362..1622)
                     /locus_tag="ACZPAQ_02780"
     CDS             complement(1362..1622)
                     /locus_tag="ACZPAQ_02780"
                     /EC_number="2.3.-.-"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0902936.1"
                     /translation="MELQFEPTRVYGLDGGGRLVAEITFPCTQPGIYTIDHTFVDPSL
                     RGQGMADKLVRAALEQIRKNGGTPAATCTYAVSWLEKHPEQA"
     gene            complement(1713..2069)
                     /gene="spoVAE"
                     /locus_tag="ACZPAQ_02785"
     CDS             complement(1713..2069)
                     /gene="spoVAE"
                     /locus_tag="ACZPAQ_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HIR0902937.1"
                     /translation="MEYFNAFLCGGILCLIGQLLIDKTPLTPAKILVLYVVTGVVLGG
                     AGVYQKLVDWAGSGATVPLTGFGYLLAKGVRKAVEEQGFLGALTGGVTAAAGGITAAI
                     FFGFLVALLFKSKPKS"
     gene            complement(2103..3119)
                     /gene="spoVAD"
                     /locus_tag="ACZPAQ_02790"
     CDS             complement(2103..3119)
                     /gene="spoVAD"
                     /locus_tag="ACZPAQ_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR0902938.1"
                     /translation="MTTKKLGRQTVALANPPTLAGHANVVGKKEGEGPLASSFDLIGS
                     DDTFGEATWEKSESAMQKQALALALDKAGQAASAMDWLFAGDLLNQCIGSSFAARGQD
                     IPFFGLYGACSTMGEGLALASMTLDGGFGEWAAVVVSSHFCTAERQYRTPLEYGGQRT
                     PTAQWTVTGAGAAILAREGPGPYVTHCTLGKIADKGITDTNNMGAAMAPAAYATLTAH
                     FQDTGRGPDYYDLIVTGDLGKLGRELLLELFRQDGVDLSTNHNDCGLMIFDLENQDVH
                     CGGSGCGCSASVLTGYLLNGMREGRWKNILFCPTGALHSPTSAFQGESVPGICHAIAI
                     SAVK"
     gene            complement(3256..4254)
                     /locus_tag="ACZPAQ_02795"
     CDS             complement(3256..4254)
                     /locus_tag="ACZPAQ_02795"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR0902939.1"
                     /translation="MTRVLLLTTGGTIASRRGDQGLEPQISARGLLEYMSDLRAHYHI
                     DARDILNLDSSNIQAEEWQTIARAVMENLDAYDGIVITHGTDTMAYTASVLSYMLQNL
                     TKPVVLTGSQMPIDNLLTDARNNLYTAFSAVDCRIPGVSIAFDHKLIRGCRAVKVRTM
                     GFEAFESVNAPYLGEVFANGIHRNESAIRPGDPSRPTRLLDRLCPDVFLLKLIPGTNP
                     AVFSMLMDMHYRGIVLETFGAGGMHFLRRNLLDRLSELTAHGVSVVACSQCLYESSDL
                     SIYEVGQRLLECGVIPGRDMTTEAAVTKLMWALGQTVDPAQGRDIFDSDLAGEVTL"
     gene            complement(4328..4912)
                     /locus_tag="ACZPAQ_02800"
                     /pseudo
     CDS             complement(4328..4912)
                     /locus_tag="ACZPAQ_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
BASE COUNT         1031 a         1545 c         1763 g          935 t
ORIGIN      
        1 cggacgcctc cggccgccgc agcccggtac ccatccaggg cagtgagttc accatggagg
       61 cggacgccgt catcatggcc ctggggacca gtccaaaccc catgagctac ggcggaactc
      121 ccggtctgga gcgcacccgg aagggctgcg tggctgcgga cgaggcggga cacacctcgg
      181 tggagggggt ctttgccggc ggcgacgccg ccaccggcgc cgccactgtc atcctggcca
      241 tgggggccgg caagcacgcc gcaaaggcca tcgacgagta tttgaagagc aagaaataag
      301 acaaagggcc ccgcagcttg tgcggggccc ttactgtaaa agtcctgttt ttggggctgc
      361 ttctgcggta ggatggaaaa aggaggggaa agacatgctt tatctgagga ggttctccct
      421 gccggggcag ggggcagagg ccagcttcct ggcacgtgac agcgagaaca aacggacctg
      481 ttacggcagc cgctacccct tcggcatctt tctgtcccga aaggtccctc ctctgacctt
      541 tgagcccatc accatcctgt gcggcgggaa cggctgcggc aagaccacgg tgctcaatct
      601 gatggcggag aagttgaagc tgcgtcgcgg gactgtgttc aaccggagct ccttttttca
      661 gccctatctg gatctctgcg aggcggagac ggcaggggcc ttcggggcgg cggagcttga
      721 gaacagccgg atcatcacca gtgacgatgt gttcgacggc ctgctggatc tccgatatat
      781 gaatgagaac gtcgagcaca ggagaaagga actgctggag gaatatgcgg atgcccggta
      841 cgcccgtttt cagatgcgct ctctggagga ctatgaccgc ttgcgccagg tggtggaggc
      901 ccagcgcaag accggctccc actatgtgcg ggaccgggtg atgaaggata tcccgggccg
      961 ttccaacggg gagagcgcat tcctttactt caccagggag atccaggagg gcgggctcta
     1021 cctgctggac gagccggaaa acagtctgtc tgcccagctc cagctggagc tggcccgctt
     1081 tctggaggac tccgcccgct tttaccgctg tcaattcgtc atctccaccc attccccctt
     1141 cctgctggcc ctgcgggggg cgaagatcta tgacctggac caggacccgg tggagccctg
     1201 ccgctggacc caggtgccca gtgtccgcac cttttacgag ttcttccgca gccgccggga
     1261 ggaatttgag gatgagaacg gcgccccgga ctgaccgggg cgccgtttca tttgtggaga
     1321 aagccatact ggggggagct ccgccgcttt ttcacgcgtt tttatgcctg ctccggatgt
     1381 ttctccagcc aggagacagc ataggtgcag gtggcggcgg gggtgccgcc gtttttacgg
     1441 atctgctcca gggcggcccg gaccagctta tccgccatcc cctgcccccg gagggagggg
     1501 tccacaaagg tgtggtcgat ggtatagatc ccgggctgag tgcaggggaa tgtgatctca
     1561 gccaccagac ggcccccgcc gtccagcccg tagacccggg taggctcaaa ttgcagctcc
     1621 atgtttatca cctcgtgccc agtgtagcac acaggtggag aaaaagccag aaaaatcctg
     1681 ctctgaggaa aagagccccc agagcggggg catcagctct tgggcttgga cttgaagagc
     1741 agcgccacca ggaagccgaa gaagatggcg gcggtgatgc cgcccgccgc tgcggtgaca
     1801 cccccggtga gggcccccag gaagccctgc tcctccactg ccttgcgcac ccccttggcc
     1861 agcagatagc cgaaaccggt gagggggacg gtggctccgc tcccggccca gtccaccagc
     1921 ttctggtaga cgcccgcgcc gcccaggacc acgccggtga ccacatagag gaccaggatc
     1981 ttggccgggg tgagaggcgt cttgtcgatg agaagctgtc cgatcaggca caggatgccg
     2041 ccgcagagaa acgcgttgaa atattccata tcagtgacct cttttcttgg acatgacggg
     2101 gatcatttga cagcggagat ggcgatggcg tggcagatac cgggcaccga ctctccctga
     2161 aaggcggagg tgggggagtg gagggcgccg gtggggcaga aaaggatatt tttccagcgg
     2221 ccctcccgca tcccgttgag cagatagccg gtgagcaccg aggcggagca gccgcagccc
     2281 gagccgccgc agtggacgtc ctgattttcc agatcgaaga tcatcagacc gcagtcgttg
     2341 tggttggtgg acaggtccac cccgtcctgc cggaacagct ccagcagcag ctcacgcccc
     2401 agcttgccca ggtcgccggt gacgatcagg tcgtagtagt ccggcccccg gccggtatcc
     2461 tgaaagtgtg cggtgagggt ggcataggcc gccggggcca tggcggcccc catgttgttg
     2521 gtgtcggtga tccccttgtc ggcgatcttg cccagggtgc agtgggtgac gtagggtccg
     2581 ggcccctccc gggccaggat ggcggccccg gcgccggtca ccgtccactg ggcggtgggg
     2641 gtgcgctggc ctccgtactc caggggggtg cggtactgcc gctccgccgt acagaagtgg
     2701 gaggagacca ccaccgccgc ccactcccca aagccgccgt ccaaggtcat ggaggccagg
     2761 gccagaccct cccccatggt ggagcaggcc ccgtagagcc cgaaaaaggg aatgtcctgc
     2821 ccccgggcgg cgaaggagga gccgatgcac tggttgagca ggtcgccggc aaagagccag
     2881 tccatggcgg aggcagcctg gccggccttg tccagcgcca gggcgagggc ctgcttttgc
     2941 atggcgctct ccgacttctc ccaggtggcc tccccaaagg tgtcatcaga gccgatgagg
     3001 tcgaaggagc tggccagagg gccctcccct tccttcttgc ccactacatt ggcgtgtccg
     3061 gccagggtgg gcgggttggc aagggccacc gtttgacggc ccagtttttt tgttgtcatg
     3121 gcgtcaagga cctccgtgct gttgaaaatc agaggtagct tgccccgaga atgaaaaaat
     3181 atgaaaaaag gcgcatgtcc cgtccgacaa aatacggcgg acgggacatg cgcagcagct
     3241 ggtggaaaaa cttttttaga gggtgacctc tccggccagg tcggagtcga agatatcgcg
     3301 gccctgcgcc gggtccacgg tctggcccag ggcccacatg agcttggtca ccgcggcttc
     3361 cgtggtcatg tcgcggccgg ggattacccc gcactccagc aggcgctggc ctacctcgta
     3421 gatggacagg tcgctggact catagaggca ctggctgcac gccaccaccg acaccccatg
     3481 ggcagtgagc tcgctcagcc ggtccagcag gttccggcgg agaaagtgca tcccgccggc
     3541 gccgaaggtc tccaggacga tgccccggta gtgcatgtcc atcagcatgg agaagaccgc
     3601 cgggttggtg ccggggatca gcttgagcag aaagacgtcg gggcacagcc gatccagcag
     3661 ccgggtgggg cggctggggt cgccggggcg gatggcgctc tcgttgcggt ggatgccgtt
     3721 ggcgaaaact tcccctagat agggggcatt gacgctctcg aaagcctcaa agcccatggt
     3781 gcgcaccttc actgcccggc agccccggat cagcttgtgg tcaaaggcaa tggagacgcc
     3841 ggggatgcgg cagtccacgg cggaaaaggc ggtatagagg ttgttgcggg cgtcggtgag
     3901 caggttgtcg atgggcatct gggagccggt gaggactacg ggtttggtga gattctggag
     3961 catgtagctc agcaccgagg cggtgtaagc catggtatca gtgccgtggg tgatgacgat
     4021 gccgtcatag gcgtccagat tttccatcac cgcccgggcg atggtctgcc actcctcagc
     4081 ctggatgttg gagctgtcca ggttgaggat gtcccgggcg tcgatgtggt agtgggcgcg
     4141 caggtccgac atatattcca gcaggccccg ggcagagatc tggggctcca gtccctggtc
     4201 gccccggcgg gaggcgatgg tgcccccggt ggtgagaagc aggactcggg tcatggaaaa
     4261 cctccttgca gaagagatgg aagggtcggg ggagggggcg taagaaccgg acgaggacct
     4321 ccgccagcgc ttcgggggcg tctgtgttca cctcgtgccc cgcaccgggg atgagggcca
     4381 gctccccctg gggcaccagc cggcagagct gccgggcggc tttctggttg gcatggtccc
     4441 ccgtgccgca gaccgccagg acaggacagc ggagccgggc caggccgcct cggaagtcca
     4501 gggccatcat ggagcgggac aggcgcatcg tatccgcctt gctcagcccg ctggcggaaa
     4561 acatggaccc gggcatcagg cggaacatgg cgttttggag ccggagcagg cccttgggca
     4621 tgacgaactg ggtgccgatc aggaccaggg ccccaacccg gtccgaatgc tcgatcccgt
     4681 attgcagagc caggaccccg cccagggaca gaccgcacag ggtgaggggg gcgggaaggg
     4741 cgccgcagta ccgtgagaag gcctgataga gattttcata acagacctcc tttccccgaa
     4801 tcagaccgaa gagatcggga cagaggccgc ctgactcctg ccccagcacg cggagcaccg
     4861 actcccagct ggccggggtt tggcccaagc catggagata gacaacagac atgggatcgc
     4921 ctcctggccc tcccggaccg gtagaggagc ggccggaaga gagtaagatc ggaccggaca
     4981 gggcccgttt tttccatcat acgatatttt tcacccgcca tcaagggaga attccgattt
     5041 tgttcggggc tggggcacgg aagagagctg actcgatccg aggcgccctt ctggccacag
     5101 cgatattaag cggaggacct gaaagccttt ttactttatc tgaaacgata aaagataatt
     5161 attttatgtt tatcattaaa aaataattga caaaaatatg ttgagtgata tactgtgtgt
     5221 taagaaatga aaggagcctt tatttatgaa agaaaaagcg ttgaaaagat atgc
//
LOCUS       DBMDSJ010000073         5232 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4423_length_5232_cov_1.705126, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000073 DBMDSJ010000000
VERSION     DBMDSJ010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4423_length_5232_cov_1.705126"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="ACZPAQ_02805"
     CDS             <1..235
                     /locus_tag="ACZPAQ_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR0902940.1"
                     /translation="RFPVVKAPGCRNEILNSKKLFLADKAGDWQRLGLWAGRLMFTTE
                     NARECVQVLERYLGRGSYQPNDYTRGLYYRGVE"
     gene            266..1390
                     /locus_tag="ACZPAQ_02810"
     CDS             266..1390
                     /locus_tag="ACZPAQ_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxE/PaaK family acyltransferase"
                     /protein_id="HIR0902941.1"
                     /translation="MFSRQRLFWHRGPYDLPSTDQLFLQATADCCAFHMARCPQYRAI
                     AEHLGFSPDQLRTMDDLVRVPMLPTLFFKRHALFSMPRRRMVMKVTSSGTSGTFSQVG
                     FDLGCIWAEVPMVLRLGWRHRLISFRPANYVVLGYKPDRRSSAGVTRTMFGMTFFAPP
                     LRRFYALRWQGEGYVPDLDGAVEALERFSRSPFPTRIVGFPSYLWFGLKRMEELGISL
                     RLRPGSKILLAGGWKQHWQQQVDKSVLYSLVRRVLGVGEEDIHELFGAVEHPIFYNTC
                     PRHHFHVPIYSRVLIRDPATLEPLPMGQVGLVNLISPLIRATPVTSVVTDDLGYLTPG
                     DACGCGLTSPYLTLLGRVGLGDIKTCAAGAADLLGKGEGF"
     gene            1387..2673
                     /locus_tag="ACZPAQ_02815"
     CDS             1387..2673
                     /locus_tag="ACZPAQ_02815"
                     /inference="COORDINATES: protein motif:HMM:NF017686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HIR0902942.1"
                     /translation="MIFANGRLLPDSQLSQVLEELEEAVNETRACRTLEPETVISALQ
                     AVGERLDRGELDPLILRYAGPGGRREVAHIRPLLRREALEYKLAVELGIPLYSFQERP
                     FGRTQTVPLGTLFHVTAGNVDGLPAFSAVEGLLTGNINLVKLPSGDQGLSLAVFQLLT
                     EQEPRLAPFLYAFQIPSRDTAALRRLADLADGAVVWGGDGAVTALRQLAPPGCKLMEW
                     GHRLSFAYLSRWEGEEDALSALAEHLIQTGGLLCSSCQVIFLDTDALGEGDAFCRSFV
                     PLLERAADRFRAALGEKGEGSLYAWETRLEHAADRLPGTLFPGRGCSLTLREDRELEL
                     SPLHGNVLVKCLPQKALLPVLRRQKGRLQTAGLLCPAQDRPALTQLLARAGVTRIAPP
                     GSMSRTLSGEGHDGEFPLRRYVRTVDIQEPSPDRPI"
     gene            2711..3160
                     /gene="dut"
                     /locus_tag="ACZPAQ_02820"
     CDS             2711..3160
                     /gene="dut"
                     /locus_tag="ACZPAQ_02820"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR0902943.1"
                     /translation="MELNVKALSPKIGTEIPAPFYASAGAAAMDLHACVDQAVTIPAG
                     GRAVIPTGIAIALPSAGYVALVFARSGLGIKHGIAPANCVGVIDSDYRGEILVGLQNS
                     GDADYVIQPADRIAQLMVVPVVQAQVKMVDQLDETQRGAGGFGSTGK"
     gene            3190..4110
                     /locus_tag="ACZPAQ_02825"
     CDS             3190..4110
                     /locus_tag="ACZPAQ_02825"
                     /inference="COORDINATES: protein motif:HMM:NF021532.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2262 domain-containing protein"
                     /protein_id="HIR0902944.1"
                     /translation="MFGFFKKKGGEIPEEQQRQQRGAQEGQTRQDAAEVPQEFRALGQ
                     DFLPEELNILAVTGASGFGGGRSQGEKLWTASIGLTAWMEEDSPDIHQGDFRLVALAD
                     DRLMDYLRQRVPRDFIIKCRARLSVDGSRLLMLELPQPGFDPDLKAILDEQKKPVSTW
                     VEGLGTFTLNRTVDWFEATVDWLGSEIALDFDRGVPEEMASAQETGRALMASQADWDH
                     RVRSYAAQELLSLANDWAADAREEEDEDSGAPLVTQEQFMERMELESIQLLPQGGFEF
                     WFNDGDLFWGHSIHVTGSLESGPESAQMEG"
     gene            4150..4746
                     /locus_tag="ACZPAQ_02830"
     CDS             4150..4746
                     /locus_tag="ACZPAQ_02830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR0902945.1"
                     /translation="MSIILASQSPRRRELLAQMGVPQFEVVPALGEEIASPGLSPAQL
                     VEVLSRQKAEEVAVQAGPDDVVIAADTVVAVDGAVLGKPRDPADAARMLSLLSGRAHT
                     VYTGVTVRRGTFSRTAHEATQVRFRPLTQSEISAYIATGEPMDKAGAYGIQGRGCVLV
                     EGIDGNYHNVVGLPTCLLAGMLRQVGIDVLTGGQEGRP"
     gene            4743..>5232
                     /gene="mreC"
                     /locus_tag="ACZPAQ_02835"
     CDS             4743..>5232
                     /gene="mreC"
                     /locus_tag="ACZPAQ_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR0902946.1"
                     /translation="MKHFLRQNGLLLLIIALLLSVLIGIVSAVMGGNADPLSNAVGFI
                     TTPIRNGVSAVLDWADGVRAYVFHYEEMEQQIQDLERQVAELEEQAREGQEASRENEQ
                     LRELLDLQAKRRDFVFESAKVTARSSTNWDSTLTIGKGSSAGLEVNDCVITETGTLVG
                     VVS"
BASE COUNT          802 a         1781 c         1776 g          873 t
ORIGIN      
        1 gcgcttccct gtggtgaaag ccccgggctg ccgcaatgag atcctcaatt ccaagaagct
       61 gttcctggcg gacaaggccg gagactggca gcgcctgggt ctctgggcag ggcgtctgat
      121 gttcaccacc gagaacgccc gggagtgcgt ccaggtgctg gagcgctacc tgggccgggg
      181 cagctatcag cccaacgact acacccgggg cctgtattac cggggggtag aataaagcgc
      241 ggaacgcgga gaggaggtcc cccctatgtt cagccggcag cgcctgttct ggcaccgcgg
      301 gccctatgac ctgcccagca cagaccagct ctttctccag gcgacggcgg actgctgcgc
      361 cttccacatg gcccgctgcc cccagtaccg ggccattgcg gagcacctgg gcttctcccc
      421 ggaccagctg cgcaccatgg acgacctggt ccgggtgccc atgctgccca ccctgttctt
      481 caagcgccac gccctcttct ccatgccccg gcggcggatg gtgatgaagg tcacctcctc
      541 ggggaccagc ggcaccttca gccaggtggg cttcgacctg ggctgcatct gggcggaggt
      601 ccccatggtc ctccggctgg gctggcggca ccggctcatc tctttccgcc ccgccaacta
      661 tgtggtgctg ggttacaagc cagaccggcg cagctctgcc ggcgttaccc gcaccatgtt
      721 cggcatgacc ttcttcgccc cgcccctccg ccgcttttat gccctccgct ggcaggggga
      781 gggctacgtc cctgacctgg acggtgcggt ggaggccctg gagcggttct cccgctctcc
      841 cttccccacc cggatcgtgg gcttcccctc ctatctctgg ttcggactga agcggatgga
      901 agagctgggc atctctctgc gcctgcggcc cggttccaag atcctgctgg ccgggggctg
      961 gaagcagcac tggcagcagc aggtggacaa gtcagtgctc tactccctgg tccggcgggt
     1021 gctgggggtg ggggaggagg acatccatga gctgttcggc gccgtagagc accccatctt
     1081 ctataacacc tgccccaggc accacttcca tgtgcccatc tactcccggg tcctgatccg
     1141 tgacccggcc accctggagc ccctgcccat gggacaggtg gggctggtca acctcatctc
     1201 ccccctcatc cgggccactc cggtcaccag cgtggtcact gacgacctgg gctatctcac
     1261 cccgggggat gcctgcgggt gcggcctgac ctccccctac ctgaccctgc tgggccgggt
     1321 gggcctgggg gacatcaaga cctgcgccgc cggcgcggcg gacctgctgg gaaaggggga
     1381 ggggttttga tcttcgccaa cggacgcctg ctcccggaca gccagctgtc ccaggtgctg
     1441 gaggagctgg aggaggcggt caatgagacc cgcgcctgcc ggaccctgga gccggagacc
     1501 gtcatctccg ccctccaggc tgtgggggag cggctggacc ggggagagct ggacccgctc
     1561 atcctccggt acgccggtcc cggcgggcgc cgggaggtgg cgcacatccg ccccctgctc
     1621 cggcgggagg ccctggaata caagctggcc gtggagctgg gcatcccgct ctactccttt
     1681 caggagcggc ccttcggccg cacccagacc gttcctctgg gcaccctgtt ccatgtcacc
     1741 gccggcaatg tagacggcct gcccgccttc agcgcagtgg agggcctgct caccggcaat
     1801 atcaatctgg tgaagctgcc ctccggggac caggggctct ccctggccgt ctttcagctg
     1861 ctgacggagc aggagccccg tctggccccc ttcctctacg ccttccagat cccatcccgg
     1921 gacaccgccg ccctgcgacg gctggcagat ctggcagacg gggcggtggt gtggggcggc
     1981 gacggggcgg tgactgccct gcggcagctg gccccgcccg gctgcaagct gatggagtgg
     2041 ggccaccgcc tcagctttgc ctatctgtcc cggtgggagg gagaggagga cgctctctcc
     2101 gctctggcgg agcacctcat ccagaccggg ggactgctct gctcctcctg ccaggtcatc
     2161 tttctggaca cggacgccct cggggagggg gatgccttct gccggagctt tgtccccctg
     2221 ctggagcggg cagcggaccg cttccgtgct gcccttgggg agaaggggga ggggagcctg
     2281 tacgcttggg agacccggct ggagcacgcc gccgaccggc tgcccggcac cctgtttccc
     2341 ggccggggct gctccctcac cctgcgggag gaccgggagc tggagctctc ccccctccac
     2401 ggcaatgtgc tggtgaagtg tctgccccag aaggctctgc tccctgtcct gaggcggcag
     2461 aagggccgtc tccagaccgc ggggttgctc tgtccggccc aggaccggcc cgccctcacc
     2521 cagctcctcg cccgggccgg agtgacccgg atcgccccgc cgggcagcat gtcccgcact
     2581 ctctccgggg agggacacga cggggaattc cccctgcggc ggtacgtccg gacggtggac
     2641 atccaggagc cgtccccgga ccgccccatc tgactgttga tactaactga ccgcaaagga
     2701 gccgatcatt atggaactga acgtcaaagc cctctccccc aagatcggga cagagatccc
     2761 cgcccccttc tacgccagcg ccggcgccgc cgccatggat ctgcatgcct gtgtggacca
     2821 ggcggtgacc atccccgccg gcgggcgggc ggtgatcccc actggcatcg ccatcgccct
     2881 cccctccgcc ggctatgtgg ccctggtctt cgcccgcagc ggcctgggca tcaaacacgg
     2941 catcgcccct gccaactgcg tgggcgtcat cgacagcgac taccggggag agatcctggt
     3001 gggactccag aactccgggg acgcagacta cgtcatccag cccgctgacc gcatcgccca
     3061 gctgatggtg gtcccggtgg tccaggccca ggtgaagatg gtggaccagc tggatgagac
     3121 ccagcggggc gccggtggct tcggctccac cgggaaatag agatccgctg agagaggagg
     3181 gtttgggcca tgttcggatt tttcaaaaaa aagggcgggg agatccccga ggaacagcag
     3241 cggcagcaga ggggggctca ggaggggcag acgcggcagg acgccgcgga ggtcccccag
     3301 gagttccgcg ccctgggcca ggactttctg ccggaggagc tgaacatcct ggccgtcacc
     3361 ggggccagcg gcttcggcgg cgggcgcagc cagggggaga agctgtggac cgcctccatc
     3421 ggcctgaccg cctggatgga ggaggacagc cctgacatcc accagggtga cttccgcctg
     3481 gtagccctgg ccgacgaccg tctgatggac tatctgcgcc agcgggtgcc ccgggacttc
     3541 attatcaagt gccgggcccg gctctctgtg gacggcagcc gcctgctgat gctggagctg
     3601 ccccagcccg gcttcgaccc cgacctgaag gccatcctgg atgagcagaa aaagcctgtg
     3661 agcacctggg tggaggggct gggcaccttc accctgaacc gcactgtgga ctggttcgag
     3721 gccaccgtgg actggctggg cagcgagatc gccctggact tcgaccgggg cgtgccggag
     3781 gagatggcct ccgcccagga gaccggacgg gccctgatgg ccagtcaggc ggactgggac
     3841 caccgggtgc gctcctatgc cgcccaagag ctgctctccc tggccaacga ctgggcggcg
     3901 gacgcccggg aggaggagga cgaggacagc ggcgcgccgc tggtcaccca ggagcagttc
     3961 atggagcgca tggagctgga gtccatccag ctgctgcccc agggcgggtt cgagttctgg
     4021 ttcaacgacg gggatctgtt ctggggccac tccatccacg tcaccggctc cctggagtca
     4081 gggccggaga gcgcccagat ggagggctga gtccccgcgc tccaattcat ggaatttcga
     4141 ggtgttctca tgtccattat cctggcttcc caatcccccc ggcggcggga gctgctggcc
     4201 cagatggggg tcccacaatt tgaagtcgtc cccgccctgg gggaggagat cgcttccccg
     4261 ggcctgtccc ccgcccagct ggtggaggtc ctctcacgcc agaaagcgga ggaggtggct
     4321 gtccaggccg ggccggacga tgtggtcatt gccgccgaca cggtggtggc ggtggacggt
     4381 gccgtgctgg gcaagccccg ggacccagcg gatgccgccc ggatgctctc ccttctctcc
     4441 ggccgggccc atacagtcta caccggggtc acggtccggc gggggacctt cagccgcacc
     4501 gcccacgagg ctacccaggt ccgcttccgc ccgctgaccc agtctgagat ctcggcctat
     4561 atcgccaccg gcgagcccat ggacaaggcc ggggcctacg gcatccaggg ccggggctgt
     4621 gtgctggtgg aggggatcga cgggaattac cacaatgtgg ttggcctgcc cacctgcctg
     4681 ctggccggca tgctgcgcca ggtggggatc gatgtgctca ccggaggaca ggaggggcgt
     4741 ccatgaaaca ttttctgcgg cagaatggcc tgctgctcct gatcatcgcc ctgctgctgt
     4801 cggtgctgat cggcatcgtg tccgccgtga tggggggcaa cgccgaccct ctctccaacg
     4861 cggtgggctt catcaccacc cccatccgca atggggtgag cgccgtgctg gactgggccg
     4921 acggcgtgcg tgcctatgtc tttcattatg aggagatgga gcagcagatc caggacctgg
     4981 agcgccaggt cgctgagctg gaggagcagg cccgggaggg ccaggaggcc agccgggaga
     5041 acgagcagct gagggagctg ctggatctgc aggccaagcg gagggacttc gtctttgagt
     5101 ccgccaaggt caccgcccgc tcctccacca actgggactc cactctcacc atcggcaagg
     5161 ggagcagcgc gggtctggag gtcaatgact gcgtgatcac cgagacaggc accctggtgg
     5221 gggtggtctc tg
//
LOCUS       DBMDSJ010000074         5226 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4426_length_5226_cov_1.424780, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000074 DBMDSJ010000000
VERSION     DBMDSJ010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4426_length_5226_cov_1.424780"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="ACZPAQ_02840"
     CDS             <1..317
                     /locus_tag="ACZPAQ_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR0902947.1"
                     /translation="VVECQQERSQFQNRDRAMRILASRLYEAEQEKRSSEFTAERRSQ
                     VGTGMRNERIRTYNFPQGRVTDHRIGLTLYKIESVMDGDLDELMDALAAADQAERLKQ
                     TQ"
     gene            348..617
                     /locus_tag="ACZPAQ_02845"
     CDS             348..617
                     /locus_tag="ACZPAQ_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902948.1"
                     /translation="MDFYIHYTALPQGKSLADVVGELNEVLEDGGAVCGGQTDGPGGR
                     LDLELEDEKVNPKFAQMAVKAYLQRAQFPRDTRVELGGMEIGVYE"
     gene            630..1286
                     /locus_tag="ACZPAQ_02850"
     CDS             630..1286
                     /locus_tag="ACZPAQ_02850"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0902949.1"
                     /translation="MFQNVIFDLDGTLLNTIDDLADAANWVCARHGWPLHTVEEYKRF
                     VGNGIPRLVERFAPAPERTPEGLARALEEFSERYGAHRADKTAPYAGMPEAVERLRAA
                     GVRTAVLSNKAHALAGPVVEDYYPGQFPWVQGALPGLPTKPDPTLLHRLMEQMGASPE
                     DTLFVGDSDVDIRTARNGGLTSCGVLWGFRGRAELEAEGADCLARTPEELVKAVLGDG
                     "
     gene            1279..1830
                     /locus_tag="ACZPAQ_02855"
     CDS             1279..1830
                     /locus_tag="ACZPAQ_02855"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0902950.1"
                     /translation="MAETILETDRLILREMTDRDFPALCRMLQDRSVMYAYEHAFSDQ
                     EAWEWLRRQQARYRKDGFGLWAAVERTSGEMVGQCGLTWQGLGDGTRIPEVGYLLERT
                     AWHKGYAVEAARACRDHAFSALKFEEVFSIIRDINLASQRVARCIGMEERGSFIKHYY
                     GMDMLHIVFSVRQGEQLPPAGSP"
     gene            2457..3476
                     /locus_tag="ACZPAQ_02860"
     CDS             2457..3476
                     /locus_tag="ACZPAQ_02860"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR0902951.1"
                     /translation="METKRLSQQDIQTAADLLRAGGLVGIPTETVYGLGANGLDPAAV
                     GRIFQAKGRPQDNPLILHIPGADWLERYCRDIPAAAYELARRFWPGPLTMVLRRRDNV
                     PDQVTAGLDTVGMRCPAHPLCRAVIQAADVPVAAPSGNTSGRPSPTTAAHMLEDMDGR
                     IDAILDGGPCSVGVESTIVDLTCTPPRLLRPGGITLEQLRAALGEVAVDPAVTRLMGA
                     GERPRAPGMKYRHYAPKAPVTVVTGEPERSAAYIAAHAGPGDGVICFEEFLPMYAGGA
                     RPVMSLGPAGDKGEQARRVFDALRAFDHTAVSAIWAQCPDSGGIGLAVTNRLNKAAGF
                     HIVEA"
     gene            3498..3971
                     /locus_tag="ACZPAQ_02865"
     CDS             3498..3971
                     /locus_tag="ACZPAQ_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902952.1"
                     /translation="MKRSHYFPWMGLIPLALGGCYVYSVAIPVVGTLLFTLLPWVMVL
                     AWAWLGMDCGRQRRPFWLSLLAGHWGVLLSVLCGAWQFWLVDDAARSIPIALFGQFIS
                     GVVPGAMMLVTPLCIVDGTYMGSLLVVWGGIASAVLLILIFTVSYWVGRRIAKAP"
     gene            3996..4427
                     /locus_tag="ACZPAQ_02870"
     CDS             3996..4427
                     /locus_tag="ACZPAQ_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902953.1"
                     /translation="MEEYTWKRFRWRMERRHARRSLLKFLFLFLALCVVLDVWGPGQI
                     ALMALLGLFVLWLLARYLRVRNEGLDGDADQLIYFDLLGRMKALPYSQIGSYTKKRSC
                     VVIRDRDGRRFAKVRKQLCSPRELEEQLREACPHLKWEGSP"
     gene            4394..5014
                     /gene="coaE"
                     /locus_tag="ACZPAQ_02875"
     CDS             4394..5014
                     /gene="coaE"
                     /locus_tag="ACZPAQ_02875"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR0902954.1"
                     /translation="MSAFEVGGITMTVIGITGPTGAGKTTVLKEIEALGGAVIDCDAV
                     YHEMLESDRSLQESLERAFGPLRDGQGAIDRKKLGALVFGDPAKLERLNAIAQRAVVQ
                     RTRQMVKENRSRGIGLTAIDAFALLESGLADLCTTTVAVLAPAEARVARIMAREGISE
                     DYAWARVRAQRPDQFFAEGCAHILYNDCGTAEEFSRKAEEFLKEIV"
BASE COUNT          895 a         1578 c         1939 g          814 t
ORIGIN      
        1 tggtggtgga gtgccagcag gagcgcagcc agttccagaa ccgggacagg gccatgcgca
       61 tcctggcttc ccggctgtac gaggcggagc aggagaagcg cagcagcgag ttcaccgccg
      121 agcgccgcag ccaggtgggc acggggatgc gcaacgagcg catccggact tataactttc
      181 cccaggggcg ggtcacggac caccgcatcg ggctgaccct gtacaagatc gagagcgtga
      241 tggacgggga cctggacgag ctgatggacg ccctggccgc cgccgaccag gcggagcggc
      301 tgaagcagac ccagtaaacg acgacgaaag gatcgggagg gaccgacatg gatttttata
      361 ttcactatac cgccctgccc caggggaaga gcctggcgga cgtggtgggc gagctgaacg
      421 aggtgctgga ggacggcggc gcggtgtgcg gcggccagac ggacggcccc ggcggacggc
      481 tggatctgga gctggaggac gagaaggtga accccaaatt cgcccagatg gcggtgaagg
      541 cctatctcca gcgggcccag tttccccggg atacccgggt ggagctgggg ggcatggaga
      601 tcggggtcta cgagtgaggc cgggccgcta tgttccaaaa tgtgatcttt gatctggacg
      661 gcactctgct caataccata gacgacctgg cggatgcggc caactgggtg tgcgcccggc
      721 acgggtggcc cctccacacg gtggaggagt acaagcgctt cgtgggcaac gggatacccc
      781 ggctggtgga gcgctttgct ccggccccgg agcggacgcc ggaggggctg gcccgggccc
      841 tggaggagtt ctcggagcgc tatggcgccc acagggcgga caagaccgcc ccctatgccg
      901 ggatgccgga ggcggtggag cggctgaggg cggcgggggt gcggacggcg gtgctgtcca
      961 acaaggccca cgcgctggcg ggcccggtgg tggaggacta ctacccgggg cagttcccct
     1021 gggtgcaggg ggccctgccc ggcctgccca ccaagccgga ccccaccctg ctgcaccggc
     1081 tgatggagca gatgggggcc tccccggagg atactctgtt cgtgggggac agcgatgtgg
     1141 acatccgcac cgccaggaac ggcggcctga ccagctgcgg cgtgctgtgg ggcttccgcg
     1201 gacgggcgga gctggaggcg gagggggccg actgtctggc ccggacgccg gaggagctgg
     1261 taaaggcggt gctgggagat ggctgagact attttagaga ctgaccgact gatactgcgg
     1321 gaaatgacgg acagagattt tcctgccctg tgccggatgc ttcaggatcg gtcagtaatg
     1381 tacgcctatg agcatgcctt ttcagaccag gaggcttggg aatggcttcg ccgccagcag
     1441 gcgcggtatc gaaaggatgg cttcggcctg tgggcagcag tggagagaac atccggggag
     1501 atggtgggcc agtgcggact cacttggcag gggctgggag atggcacacg gatacccgag
     1561 gttggctatc ttctggagcg gacggcctgg cacaagggct acgccgttga ggcggcccgg
     1621 gcctgccggg accatgcctt ttcagccctg aaatttgagg aggtattctc catcatccgg
     1681 gacatcaacc ttgcatccca aagagtagct cggtgcatcg ggatggagga gcggggctct
     1741 ttcataaagc actactatgg gatggatatg ctccacatcg tgttctccgt ccggcagggg
     1801 gaacagcttc cgcctgctgg aagcccttga ggggccccat agaagctccc acacaccttt
     1861 ccttagagga aaggtgtcgc ggcatatgcc gtgtccccac ctcaagcctt ccccccagag
     1921 gggggaaggt ggcacggcga agccgtgacg gatgaggggg cgtccttcca ccgggccggt
     1981 ctcagtcaca ggatttgtca ggggggttcc gccccgcagg gcggaatcca tttggggaca
     2041 ccctgccgcc gaagccgggc aggccaagcc cgcccctgca caaagaccga aaacatcccg
     2101 aaggggcggc ttccagctgc ccggaaagcc tttccccctc tggggggaag gtggcacggc
     2161 gaagctgtga cggatgaggg ggcgtccttc caccgggccg gtctcagtca caggatttgt
     2221 caggggggtt ccgccctgcg gggcggaatc catttgggga caccctgccg ccgaagccgg
     2281 gcgggccaag cccgccccta cgcagggcgc gggccccata gggagaccgg cggggaaaac
     2341 ggggtccgac gaagcagcgc gccgggccgc tgtgcgctgc acagggggca cgccccgccc
     2401 ggatggaacc ctcggaaaac gggcacgcgc ccaagagaga taggaagtgg aaaaggatgg
     2461 agacaaagcg cctctcccaa caggacatcc agaccgccgc ggacctgctc cgcgccggcg
     2521 gcctggtggg catccccacc gagacggtgt acggcctggg ggccaacggg ctggaccccg
     2581 ctgcggtggg ccggatcttt caggccaagg gccgccccca ggacaacccc ctgatcctgc
     2641 acatcccggg ggcggactgg ctggagcggt actgccggga catcccggcg gcggcctatg
     2701 agctggcccg gcgcttctgg cccggtccgc tgaccatggt gctccggcgg cgggacaacg
     2761 tgcccgatca ggtgacggcg gggctggaca cggtgggaat gcgctgtccc gcccaccccc
     2821 tgtgccgggc ggtgatccag gcggcggacg tgccggtggc ggccccctcg gggaatacct
     2881 ccgggcggcc cagccccacc accgccgccc acatgctgga ggacatggac ggcaggatcg
     2941 acgccatttt ggacggcggc ccctgctccg tgggggtgga gtccaccatc gtggacctga
     3001 catgcacgcc cccccgcctg ctgaggccgg gggggatcac cctggagcag ctgcgggcgg
     3061 ccctggggga ggtggcggtg gacccggcgg tcacccggct gatgggggcg ggggagaggc
     3121 cccgggcccc tgggatgaag taccgccact acgcccccaa ggcccccgtc accgtggtca
     3181 ccggggagcc ggagcggagc gcggcctata tcgccgccca cgccggaccg ggggacgggg
     3241 tcatctgctt tgaggagttc ctcccgatgt atgcgggggg cgcgcggccc gtcatgagcc
     3301 tggggccggc gggggacaag ggggagcagg cccggcgggt gttcgacgcc ctgcgggcct
     3361 tcgaccacac cgccgtctcc gccatctggg cccagtgccc ggacagcggc gggatcgggc
     3421 tggcggtcac caaccgcctg aacaaagcgg cgggcttcca catcgtggag gcctgaccga
     3481 ggaaaggagc gagggcagtg aagcggtccc actatttccc ctggatgggg ctgattcccc
     3541 tggccctggg gggctgctat gtctactcgg tggccatccc ggtggtgggg acgctgctgt
     3601 tcaccctgtt gccctgggtc atggtgctgg cctgggcctg gctggggatg gactgcgggc
     3661 ggcagaggcg gcccttctgg ctcagcctcc tggccggcca ctggggggtg ctcctcagcg
     3721 tgctgtgcgg ggcgtggcag ttctggctgg tggacgacgc cgcccggagc atccccatcg
     3781 ccctcttcgg ccagtttatc tccggcgtgg tgcccggggc gatgatgctg gtcacccccc
     3841 tgtgcatcgt ggacggcacc tacatgggca gcctgctggt ggtgtgggga ggcatcgcct
     3901 cggcggtgct gctgatcctc attttcaccg tgtcctactg ggtgggccgc cgcatcgcca
     3961 aggccccctg accggcccgg aaaggaggtc cttcgatgga agagtacaca tggaaacgct
     4021 tccggtggcg tatggagcgc aggcacgccc ggcgctccct gctgaaattt ctcttcctct
     4081 ttctcgcgct gtgtgtcgtg ctggacgtat gggggccggg gcagattgcc ctgatggccc
     4141 ttctggggct gttcgtcctg tggctgctgg cgcggtatct gcgggtgcga aacgagggac
     4201 tggacggcga cgcagatcag ctgatctatt tcgacctgct gggtcgcatg aaggccctgc
     4261 catattccca aatcgggagc tataccaaaa agcggagttg tgtggtgata cgggaccggg
     4321 acggccggag gttcgccaag gtccggaagc agctgtgcag cccccgggag ctggaggagc
     4381 agctccggga ggcatgtccg catttgaagt gggagggatc accatgactg tcatcggcat
     4441 caccggccct accggggcgg ggaagaccac cgtcctgaag gagatcgagg cactgggggg
     4501 cgcggtcatc gactgcgatg ccgtgtacca cgaaatgctg gaaagtgata gatccttaca
     4561 ggaaagcctg gagagggcct ttggccccct ccgggacgga cagggggcca ttgacagaaa
     4621 aaagctgggc gccctggtgt tcggggaccc ggccaagctg gagcggctga acgccatcgc
     4681 ccagcgggcc gtggttcagc gcacccggca gatggtcaaa gagaacaggt cccggggcat
     4741 cggcctgacg gccatcgacg ccttcgccct gctggagagc ggtctggccg acctgtgcac
     4801 caccacggtg gccgtactgg ccccggcgga ggcgcgggtg gcgcgcatca tggcccggga
     4861 gggcatttcg gaggactatg cctgggcccg ggtgagggcc cagcggccgg accagttctt
     4921 tgcggagggc tgtgcccata tcctgtacaa cgactgcggc accgcggagg agttttcccg
     4981 gaaggcagag gagtttttga aagagatcgt gtaggggcgc acagtgtgcg cccgtgcgcg
     5041 gcataataag agaacaatga ggcgcgcaaa gcgcccctgt gagatcaaag gaggactgtt
     5101 ttatgagtga agaaaaagaa ctgacccggg gcgagcagct ccgccgggcc ctggtctatg
     5161 agcggaagaa cggctgggac cggctggacg cggaggaggg cgcggcgctt gaggcgtact
     5221 gcaccg
//
LOCUS       DBMDSJ010000075         5167 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4492_length_5167_cov_1.438808, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000075 DBMDSJ010000000
VERSION     DBMDSJ010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4492_length_5167_cov_1.438808"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(235..756)
                     /locus_tag="ACZPAQ_02880"
     CDS             complement(235..756)
                     /locus_tag="ACZPAQ_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902955.1"
                     /translation="MASGVEELLDMLFEMVDEAKNMPLSSDKCVLERDRALDLIDDIR
                     AQFPVELGEAKKLVASRNDYIAAAKREAEVIRKQAEAEAQRMLAEESVLNQAKQRSAE
                     LMRQAEERSRELKRSANEYCEDALRRTEEAVAEAYDEIKKSRARFRAAASATAAGSPS
                     GGRAAYDAAAEED"
     gene            complement(756..1256)
                     /gene="coaD"
                     /locus_tag="ACZPAQ_02885"
     CDS             complement(756..1256)
                     /gene="coaD"
                     /locus_tag="ACZPAQ_02885"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR0902956.1"
                     /translation="MKTAIYPGSFDPVTLGHLNIIKRAAQCFDRVIVCVMVNSKKHGL
                     FTPEERVELLERCTARFPNVEVDFSDELLAAYAKRRKAHVVVKGLRAVSDFEQEVQMA
                     VINRKLNPRLETMFLASSDKYTYLSSTVVKEMARYGADLGEFVPREIVDDVDRKMRQA
                     QEKEND"
     gene            complement(1253..1813)
                     /gene="rsmD"
                     /locus_tag="ACZPAQ_02890"
     CDS             complement(1253..1813)
                     /gene="rsmD"
                     /locus_tag="ACZPAQ_02890"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR0902957.1"
                     /translation="MRVISGSARGRRLKELQGMDTRPTTDKVKESLFNIIQFEIEGRS
                     VLDLFGGTGQLGIEALSRGAERCTFVDARREAAALIRENLKATGLEKAGRVVQGDAIS
                     FLSGCRERFGVIFLDPPYQTQLLEQTLELVSKIDILSEHGIMICESPAERELPPLAPP
                     YEKGREYRYGRIKLTVYRRQGSDPRP"
     gene            complement(1816..3672)
                     /gene="uvrC"
                     /locus_tag="ACZPAQ_02895"
     CDS             complement(1816..3672)
                     /gene="uvrC"
                     /locus_tag="ACZPAQ_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR0902958.1"
                     /translation="MTFDELKEKAHALPLKPGVYIMQDAKNTVIYVGKAKALKNRVSQ
                     YFQDSASHTEKTRAMVSQIDHFDVIVADSEFEALVLENSLIKRHQPRYNILLKDDKGY
                     PYIRLSGEEYPRFSLANRAAEDGARYFGPYASRHATQAIVDALRSALRLPSCGKRFPR
                     DIGKERPCLNFHMGKCDGYCRSLQMKEQHEEAIAQAVSLLEGRFKEVKADLTAEMERA
                     AEELRFERAAELRDRIQAIELLGMRQKVVAGSLADTDVTGFFRGTAKSCFVVLHYMEG
                     ELAAKDMDLLETPMEEDEEEVLSSLVREYYVGRSRLPRQILLPCELPDQVPLTRMLSE
                     QVGRRVELVTPQRGAKMDLIRLANQNAAEEVARATTREERQNKLLEALARMLGMEEPP
                     RRMESYDISNTGASDIVASMVVYVDGRPLKRDYRRFKLKDMEGPDDYASMEQVLTRRF
                     RRYLDGDEKFVEKPDLLLIDGGLEHVRVARRVLEAMALDIPAFGMVKDDRHRTRALVH
                     PDGREIGIQAVPAVFSLIGQIQEETHRFAIEYHRQLQAGHVKGSVLDKIPGVGEKRRA
                     QLLKHFKSVKAIRQASLEELERAVPQSTAQAVYQYFRRGETENQDQAERKEE"
     gene            complement(3745..4008)
                     /locus_tag="ACZPAQ_02900"
     CDS             complement(3745..4008)
                     /locus_tag="ACZPAQ_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR0902959.1"
                     /translation="MYSQEAVVNNQVGLHARPATFFIQKANEFKSSIWVEKDERKVNA
                     KSLLGVLSLGIVKGTPIKLIADGPDEKEAVEALFKMISSDFSE"
     gene            complement(4221..>5167)
                     /locus_tag="ACZPAQ_02905"
     CDS             complement(4221..>5167)
                     /locus_tag="ACZPAQ_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR0902960.1"
                     /translation="YRLVMGIPGKSNAFAISRRLGLPEEIIDRAAARLDAENVRFEDV
                     LTKLDQQRQEMEKDRAEARRLKLEMEQSAGKAREYRKRLEEERSKVVEKAQSEARAII
                     QEARDASDLALSELKELKKRQDLDWQQVNDGRAEARRLLNEAERSIGGAAQEPEAPPP
                     TRPARAGDTVELVSMGTRASVLSVNKDGTLQLQAGILKITAKQDEVRVVEGETQSQKE
                     ARRIVQRAQHTLRAAAAPSEIDLRGMMTDEAIAVLDRFLDTAMMGKLESVTIIHGKGT
                     GAVRKAVREHLKRSRYIKSFRPGRYGEGEDGVTVAELR"
BASE COUNT          815 a         1806 c         1465 g         1081 t
ORIGIN      
        1 caactttccc ctttttgtcc attccgacgc ttttttctcc atactgccgg agcgtccagc
       61 gcctcccgcc ggctgatttt tgttattgta aacctctctt ctccaaatgt caagaaaatt
      121 ttttgtaaaa aatgaaggcc tgccccatcc ccccttcatt ttcccgggag gcccacccac
      181 cgtggcacaa agcgggggcc gcacatccgt gcggcccccg ctgttctctg tgattcagtc
      241 ctcttcggcc gccgcgtcat aggcagcccg ccctcccgac gggctccccg cggcggtggc
      301 gctggccgcc gcccggaagc gggcccggga cttcttgatc tcgtcatagg cctccgccac
      361 cgcctcctcg gtgcggcgca gggcgtcctc gcagtactcg ttggcggagc gcttcaactc
      421 ccggctgcgc tcctccgcct ggcgcatcaa ttcagcagag cgctgcttgg cctggttcag
      481 cacggactcc tctgccagca tgcgctgcgc ctccgcctcc gcctgcttgc ggatcacctc
      541 cgcctcccgc ttagccgcgg cgatatagtc gttccgggag gccaccagct tcttagcctc
      601 gcccagctcc acagggaact gggcccggat gtcatcgatc agatccaggg cgcggtcccg
      661 ctccagcacg cacttgtcgc tggaaagggg catattcttg gcctcatcca ccatttcaaa
      721 gagcatgtcc agcagctctt ccacaccgct tgccatcagt cattctcctt ttcctgcgcc
      781 tggcgcattt tccggtctac gtcgtccacg atctcccggg ggacgaactc ccccaggtct
      841 gctccatacc gggccatctc cttcaccacg gtggagctga ggtaggtgta cttatcgctg
      901 gaggccagga acatggtctc cagcctgggg ttcagcttgc ggttgatgac cgccatctgg
      961 acctcctgct caaagtcgga taccgcccgc aggcccttca ccaccacatg agccttcctg
     1021 cgcttggcat aggcggccag cagctcgtcg gagaagtcaa cttccacgtt gggaaagcgg
     1081 gcagtacacc gttccagcag ctccacccgc tcctccgggg taaacagccc gtgcttcttg
     1141 gaattcacca tcacacagac gatcacccgg tcaaagcact gagcggcccg cttgataatg
     1201 ttcagatgcc ccagggtgac agggtcaaag ctgccgggat agatagcggt cttcatggtc
     1261 tcgggtcact cccctgacgg cggtaaactg tcagtttgat cctgccgtac cggtattccc
     1321 tccccttttc atagggaggt gccagcgggg gaagctccct ctccgcggga ctttcgcaaa
     1381 tcattatacc atgttccgaa agaatgtcaa tcttcgacac cagctccaga gtctgctcca
     1441 gcagctgggt ctgatagggc gggtccagga atatgacacc aaaccgctcc cggcacccgc
     1501 tgaggaagga gatcgcatcc ccctgcacca ctcggcccgc cttctccagt ccggtggcct
     1561 tcaggttctc ccggatgagg gcagcagcct cccgccgggc gtccacaaag gtgcatcgct
     1621 ccgccccccg ggacagagcc tcgatcccca gctgtccggt gccgccgaac aggtccagga
     1681 cgctccgccc ctcgatctcg aactggatga tgttgaacag ggactctttc accttgtcgg
     1741 tggtgggccg ggtgtccatc ccctggagct ctttcagtct tctgccccgt gcggagccgg
     1801 agatcactcg catggtcact cttcctttct ctcagcctga tcctgatttt ctgtctctcc
     1861 cctgcggaaa tactggtaca ccgcctgggc cgtgctctgg gggaccgccc gctccagctc
     1921 ctccagagat gcctgccgga tggccttcac gcttttgaag tgcttgagca gctgggcccg
     1981 gcgcttttcc cccaccccgg gtattttgtc cagaacggag ccttttacgt gtcccgcctg
     2041 gagctgccgg tggtattcga tggcgaagcg gtgggtctcc tcctggatct gcccgatgag
     2101 ggagaacact gccggcaccg cctggatacc gatctcccgc ccgtcggggt ggaccagggc
     2161 cctggtgcgg tgccggtcat ccttcaccat gccgaaggcc gggatgtcca gcgccatggc
     2221 ctccagcacc cgcctggcca cccgcacatg ctccagaccg ccgtcaatga gcagcaggtc
     2281 gggcttctcc acaaattttt cgtccccgtc cagataccgg cggaaccgcc gggtcagcac
     2341 ctgctccatg gaggcgtagt cgtccgggcc ctccatatcc ttcagcttaa agcggcggta
     2401 gtcccgcttc aggggccttc cgtccacata gaccaccatg gaggccacga tgtcactggc
     2461 tccagtgttg gagatgtcgt aggactccat ccgccggggg ggctcctcca tgcccagcat
     2521 cctggccagc gcctccagca gcttgttctg acgttcctcc cgggtggtgg cccgggccac
     2581 ctcttctgcc gcgttctggt tggccagccg gatcagatcc atctttgccc ccctctgtgg
     2641 ggtcaccagc tccacccggc ggcccacctg ctcggagagc atccgggtca gcgggacctg
     2701 gtcgggcagc tcacagggca gcaggatctg cctgggcagc cggcttcgtc ccacatagta
     2761 ctcccgcacc agggaggaga ggacctcctc ctcgtcctcc tccatagggg tctccagcag
     2821 gtccatatcc ttggccgcca gctccccctc catgtagtgc aggaccacga agcagctctt
     2881 ggctgtcccc cggaaaaagc cggtgacgtc ggtatccgcc agggagcccg ccaccacctt
     2941 ctgccgcatc cccagcagct cgatggcctg gatgcggtcc cgcagctccg ccgcccgctc
     3001 gaagcgcagt tcctccgccg cccgctccat ctcggcggtc aggtccgctt tgacctcctt
     3061 gaaccgcccc tccagcaggg agaccgcctg ggcgatggcc tcctcatgct gctccttcat
     3121 ctggaggctg cggcagtagc catcgcactt gcccatgtgg aagttgaggc aggggcgctc
     3181 cttcccaatg tcccggggaa agcgcttccc acaggagggc aggcgcaggg cggagcgcag
     3241 ggcgtccacg atagcctggg tggcgtggcg gctggcgtaa gggccgaaat agcgtgcccc
     3301 gtcctccgcc gcccggttgg ccagggagaa gcgggggtac tcctccccgg acaagcggat
     3361 gtaggggtag cccttgtcgt ccttgagcag gatgttgtac cggggctggt ggcgcttgat
     3421 gagggagttc tccagtacca gggcctcaaa ctcgctgtcc gccacgatca cgtcaaagtg
     3481 gtcgatctgg gacaccatgg cccgggtctt ctccgtgtga gaggcggagt cctggaaata
     3541 ctgggacacc cggtttttca acgccttggc cttgcccaca tagatcaccg tgtttttggc
     3601 atcctgcatg atatacacgc ctggcttcag aggcagggcg tgggcctttt ccttcaattc
     3661 gtcaaatgtc atagggggcc tcccggggag atgttaaaaa aggcaccgcg cgaagcggtg
     3721 cctttcggat ctgcgctgtg tggctcactc ggaaaaatcg gaagagatca tcttgaacag
     3781 agcctccacc gcttccttct catccggacc atccgcaatg agcttgatag gagtaccttt
     3841 gacaataccc agggagagca cgcccagaag gctcttggcg ttcaccttgc gctcgtcctt
     3901 ctccacccaa atggagcttt tgaactcgtt ggctttctgg ataaagaacg tggcgggtct
     3961 ggcatgcagg cctacctggt tattgacgac cgcttcctga ctatacatat ccgtacctcc
     4021 gcactcgatt tgtgtgttac tagcatacca aattcatccc gcatccgtca atgacatttt
     4081 tcacaaatct gccttctttt ctcaacaaaa attgcgggag gtccacggca ttcggcggca
     4141 gatgcccttc ttcccggtct ccgcccaccg ctgggccggc cgtgcgcgct tctgtccccc
     4201 tggaaagagg gcccggcccc ttaccgcagc tccgccacgg tgacgccgtc ctccccctcg
     4261 ccgtagcggc cggggcggaa ggatttgata tagcggctgc gcttcaaatg ctcccgcacc
     4321 gccttgcgca ccgcgccggt cccctttcca tggatgatgg tcacgctctc cagcttgccc
     4381 atcatagcag tatccaggaa gcggtccagc accgcgatgg cctcgtcggt catcatgccc
     4441 cgcagatcga tctcagaggg ggctgcggcg gcgcgcaggg tgtgctgggc ccgctggacg
     4501 atgcgcctgg cctccttctg gctctgggtc tcgccctcca ccacccgcac ctcgtcctgc
     4561 ttggcggtga tcttcaagat ccccgcctgg agctggaggg tgccgtcctt gtttaccgac
     4621 aggacgctgg ctctggtccc catggatacc agctccaccg tatccccagc ccgggccggc
     4681 cgggtggggg gcggggcctc cggctcctgc gccgccccgc cgatggaccg ctccgcctcg
     4741 ttgagcaggc ggcgggcctc cgcccgtccg tcgttcacct gctgccagtc caggtcctgg
     4801 cgctttttca gctccttcag ttcggagagg gccaggtcgc tggcgtctct ggcctcctgg
     4861 atgatggccc gggcctccga ctgggccttc tccaccacct tggagcgctc ctcctccagc
     4921 cgcttccggt actcccgggc cttgcccgca gactgctcca tctccagctt cagccgccgg
     4981 gcctccgccc ggtccttctc catctcctgg cgctgctggt ccagcttggt gagtacgtct
     5041 tcaaagcgca cgttctccgc atccagccgg gcggccgccc ggtcgatgat ctcctccggc
     5101 aggcccaggc gccgggagat agcgaaggcg ttggatttgc ccgggatgcc cattaccagc
     5161 cggtagg
//
LOCUS       DBMDSJ010000076         5129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4533_length_5129_cov_1.825776, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000076 DBMDSJ010000000
VERSION     DBMDSJ010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4533_length_5129_cov_1.825776"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="ACZPAQ_02910"
     CDS             complement(<1..1240)
                     /locus_tag="ACZPAQ_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR0902961.1"
                     /translation="MPECRFTSTALGALRLAQENAARLGHSYVGSEHLLLGLASQEYS
                     AAARSLQSSGVDSRSLRSAISQLVGTGVPARSLHQGLTPKCCLAIQQAAAASRRLGQN
                     AVSAEHLLLGLLQDRECAACRLMAEQGADLDQLSQGLAAFLGGEELPTRSVRPREPER
                     STDTRLLDQCARDLTRMAADGQLDPVIGREEELTRVIQILSRRTKNNPALIGEPGVGK
                     TAIVEGLALAIADGTAPSHLLGRRVCALDLCAMVAGTKYRGEFEEKLKHVLEEVRRAG
                     NIILFIDELHTIVGAGSAEGAIDAANILKPALSRGELQVLGATTLDEYQRYIEKDAAL
                     ERRFQPVTVREPTRDQALAILQGLRGRYEGHHHLSISDKALEAAVDLSCRYLPQRFLP
                     DKAIDLVDEAASRARLAGRSL"
     gene            1356..1526
                     /locus_tag="ACZPAQ_02915"
     CDS             1356..1526
                     /locus_tag="ACZPAQ_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR0902962.1"
                     /translation="MAYKISDACISCGSCADGCPVGAISQGDSQYVISEDACIDCGAC
                     SDTCPVGAISQG"
     gene            1775..2215
                     /locus_tag="ACZPAQ_02920"
     CDS             1775..2215
                     /locus_tag="ACZPAQ_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR0902963.1"
                     /translation="MSGGAKYFIVEAEAMPEIIRKVAEAKRLLETGEKTTVNGAAREV
                     GISRSAFYKYKDAVHPFQDMLHGRIVTLQTVLRDEPGVLSGVLNVFAGTGINILTINQ
                     NIPVNGCAVVTITAETSALRRSLEELLAAAGGSPGVLRCEILAG"
     gene            2265..>3186
                     /locus_tag="ACZPAQ_02925"
     CDS             2265..>3186
                     /locus_tag="ACZPAQ_02925"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR0902964.1"
                     /translation="MVNVAILGFGTVGSGVAEVLTTNGGLIDQRVENLVRLKYILDVR
                     DFPDSPYRDCFVKDFGVIESDPEVDIVVETIGGATVALDFTTRALKAGKSVVSSNKEL
                     VATHGYELLQLAREHGVSYLFEASVGGGIPILRPLTSCLAANELSQVTGILNGTTNYI
                     LTRMIKAGLTFEQALKEAQENGYAERDPSADVDGHDACRKICILAALAFGQHVYPDQV
                     PTQGIRGVTLADVAYADSCGYKIKLLGRAIREGEGKLCAFVAPHLVPASNPLSGVEDV
                     FNAISVTGNAIGDVMFYGRGAGKLPTASAVV"
     assembly_gap    3187..3196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3197..3470
                     /locus_tag="ACZPAQ_02930"
     CDS             <3197..3470
                     /locus_tag="ACZPAQ_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR0902965.1"
                     /translation="AKDLKTARCAVWGPGSRDLVVSSDILSSRWYVRGTASMDKVRAA
                     FGEISPLARGGAPAGETAFLTAPMTEPQVRARLEGMELHSLIRVLE"
     gene            3548..4774
                     /locus_tag="ACZPAQ_02935"
     CDS             3548..4774
                     /locus_tag="ACZPAQ_02935"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR0902966.1"
                     /translation="MALIVQKFGGSSVADTARIRNVARIITETYRKGNNVVVVLSAQG
                     DTTDDLIAKAEEINPGASRREMDMLLATGEQISISLCAMAIEALGYPVISLTGWQAGM
                     LTNSAYGSARIKRIRTDRIQTELDARKIVLVAGFQGINKYDDITTLGRGGSDTSAVAL
                     AASLHADLCQIYTDVDGVYTADPRSVTGARKLEEITYDEMLELASLGAQVLHNRSVEM
                     AKRYNVNLEVLSSFTGNPGTKVKEVVKNMEKSHVSGVAKDKNVARLALVGLADEPGIA
                     FKIFSLLAKNRINVDIILQSIGRDSSKDISFTVARGDTERAKALLEEHREAIGFREIE
                     VNEQVAKVSIVGAGMVNNPGVAARMFEALYGASVNINMISTSEIKVSVLVNERDADRA
                     VQAIHDRFFNEFGGNA"
BASE COUNT          990 a         1520 c         1674 g          935 t
ORIGIN      
        1 ggagggagcg cccggccagc cgggcccggc tggccgcctc gtccaccagg tcgatggcct
       61 tgtccgggag aaagcgctgg ggcagatagc ggcaggacag gtccaccgcc gcctccaggg
      121 ccttatcgga gatggagagg tggtggtgcc cctcgtatcg gccccgaaga ccctgtagga
      181 tggccagggc ctggtcacgg gtgggctccc ggaccgtgac gggctggaag cggcgctcca
      241 gggcggcgtc cttctcgata tagcgctgat actcgtccag tgtggtggcc cccagcacct
      301 ggagctcccc acgggacaga gcgggcttca ggatgttggc ggcgtcgatg gccccctccg
      361 cgctccccgc ccccacgatg gtgtgcagct catcgatgaa gaggatgatg ttgcctgccc
      421 ggcggacctc ctccagcacg tgtttgagct tctcctcgaa ctccccacgg tacttggtgc
      481 ccgccaccat ggcgcatagg tccagagcgc acacacgcct gcccagcagg tgggaggggg
      541 cggtgccatc agcgatggcc agggccagcc cctccacaat ggcagtcttt cccacgcctg
      601 gttccccgat gagggcgggg ttgttcttgg tccgccggga caggatctgg atcacccggg
      661 tcagttcctc ttcccggccg atcaccgggt ccagctgtcc gtcggcagcc atccgggtca
      721 agtcccgggc gcactggtcc agcaggcggg tatcggtgct gcgctccggt tcccggggtc
      781 ggacggagcg ggtggggagt tcctcgcccc ccagaaaggc tgccagtccc tgggagagct
      841 ggtccagatc cgccccctgc tccgccataa ggcggcaggc ggcgcactca cggtcctgga
      901 gcagacccag cagcaggtgc tccgcgctga cagcgttctg ccccaggcgg cgggaggcgg
      961 ccgctgcctg ctggatggcc aggcagcact tgggggtcag gccctggtgg agggaccgag
     1021 cgggcacgcc ggtgcccacc agctgggaga tggcggagcg cagggagcgg ctgtccaccc
     1081 cgctgctctg aagggaccgg gcggcggcgc tgtactcctg gctggccaga cccagcagca
     1141 ggtgctcgct gcccacatag ctgtgcccaa gccgggcggc gttctcctgg gccagccgca
     1201 gggcgcccag ggcggtggag gtaaagcggc attctggcat acatatcacc tctggatagg
     1261 ttttcctctc agtatttcca atttcaaaaa aattatcatt gcatttttca aaacttgtga
     1321 taaaatgaga ctagattttt atggaggtgt ttcccatggc ttacaagatt agcgatgcct
     1381 gcatcagctg cggctcctgc gccgacggct gccctgtggg tgcgatttct cagggcgatt
     1441 cccagtatgt catcagcgag gatgcctgca tcgactgcgg cgcttgctct gacacctgcc
     1501 ctgtgggtgc catttcccag ggctgattgg caacaaaaaa acagcgccgg cgtgtgcggc
     1561 gctgtttttt tatgctcaaa agggcctgaa aagcaggaga ctgtgtaata tgacggatat
     1621 tttcggcggg acgggaagaa aatctctatt tttcgtcaaa gatttcattg aagcggtccg
     1681 ccgggtgtgt tacaatggac cacaacacaa gaacaaatgc ccttgcaaca cggacacggg
     1741 gcggggatga cgcatagtct ggagggagac gcagttgagc ggaggggcga agtactttat
     1801 cgtggaggca gaggccatgc cggagatcat tcggaaagtg gcggaggcca agcgcctgtt
     1861 agagaccggg gagaagacca cggtgaacgg cgcggcccgg gaggtgggca tcagccggag
     1921 cgcgttctat aagtataagg acgcggtgca tccctttcag gatatgctcc acggcaggat
     1981 cgtcacactc cagaccgtac tgcgggatga gcccggcgtg ctctccggcg tgttgaacgt
     2041 gttcgccggt acaggcatca atattctgac catcaatcag aacatcccgg tcaacggctg
     2101 tgcggtggtg accatcacgg cggagacctc cgccctgcgg cgctccctgg aggagctgct
     2161 ggccgccgcg gggggcagcc ccggcgtgct gcgctgtgag atcctggccg gataacgggc
     2221 cgtgttttta gattatcgtg gaagagattc atgaggtgaa cggaatggta aatgttgcga
     2281 tcttaggctt tggcactgtg ggctccggcg tggcagaggt gctcaccacc aacggcgggc
     2341 tgatcgacca gcgggtggag aatctggtgc ggctgaaata tatcctggat gtgcgggatt
     2401 tcccggacag cccgtaccgg gactgcttcg tcaaggactt tggggtcatc gagagcgacc
     2461 ccgaggtgga catcgtagtg gagactatcg gcggcgccac ggtggcgctg gactttacca
     2521 cccgggcgct gaaagcgggc aagagcgtgg tctcctccaa taaggagctg gtggccaccc
     2581 acggctatga gctgctccag ctggcccggg agcacggggt cagctatctg ttcgaggcca
     2641 gcgtgggcgg gggcatccct atcctgcggc ccctgacctc ctgcttggca gccaacgagc
     2701 tcagccaggt caccggcatc ctcaacggca ccaccaacta tatccttacc cggatgatca
     2761 aggcgggcct caccttcgag caggcgctga aggaggcaca ggagaacggt tacgcggagc
     2821 gggacccctc cgccgacgta gacggacacg acgcctgccg gaagatctgc atcctggcag
     2881 ccctggcctt cgggcagcac gtctaccctg accaggtgcc cacccagggc atccggggcg
     2941 tcaccctggc cgatgtggcc tatgccgact cctgcggcta taagatcaaa ctgctgggcc
     3001 gggccatccg ggagggggag ggaaagctct gcgcctttgt ggccccccat ctggtgcccg
     3061 cctccaaccc cctgtccggg gtggaggacg tgttcaacgc catttccgtc accggaaacg
     3121 ccatcggcga cgtgatgttt tatggccggg gagcgggcaa gctgcccacg gcttccgccg
     3181 tggtggnnnn nnnnnntgcc aaggatctga agactgcccg gtgtgcggtc tggggacccg
     3241 ggtcccggga cctggtggtc agttccgaca tcctcagctc ccgctggtat gtccggggga
     3301 cggcctccat ggacaaagtc cgggccgcct tcggggagat cagccccctg gcaaggggag
     3361 gtgcgcctgc cggggagacc gctttcctga ccgcacccat gacggagccc caagtccggg
     3421 ccaggctgga ggggatggag ctccactccc ttatccgggt gctggagtga ggaacgccgc
     3481 cggcatagaa tggtgtgcag agtcctgcgc gctgatcgag aattgacctt tagggggaaa
     3541 cagatatatg gcattgatcg tacaaaaatt cggcggcagc tccgtcgccg acacggcccg
     3601 catccggaat gtggcccgga tcatcacgga gacatatcgc aaaggaaaca acgtggtggt
     3661 ggtcctgtcc gcccaggggg acaccaccga tgacctgatt gccaaggcgg aagagatcaa
     3721 ccccggagcc tcccgccggg agatggacat gctgctggcc actggagaac agatctccat
     3781 ctccctgtgc gccatggcca tcgaagctct ggggtatcct gtgatctctc tcactggctg
     3841 gcaggcgggg atgctcacca actccgctta cggcagtgcc cggatcaagc gcatccgcac
     3901 cgaccgcata cagactgagc tggacgcccg gaagatcgtc ctggtggccg gcttccaggg
     3961 catcaataag tatgatgaca tcactaccct gggacggggc ggctccgaca cctcggcggt
     4021 ggctctggcc gcctccctcc atgcagatct gtgtcagatc tacaccgatg tggacggcgt
     4081 gtacaccgcc gatccccgct cggtcaccgg agcgagaaag ctggaagaga tcacctacga
     4141 cgagatgctg gaactggcca gcctgggggc gcaggtgctg cacaaccgct cggtggaaat
     4201 ggccaagcga tacaacgtca atctggaggt tctctccagc ttcaccggga atccgggtac
     4261 gaaagttaag gaggtcgtaa aaaacatgga aaaatcccac gtcagcggtg tggcgaaaga
     4321 taaaaatgtg gcccggctgg ccttggtggg cctggccgac gagcccggga tcgcctttaa
     4381 gatcttctct ctcttggcaa agaaccggat caacgtagac atcatcctcc agtccattgg
     4441 acgggacagc agcaaggata tcagcttcac cgtggcccgg ggcgacactg aacgggccaa
     4501 ggcgctgctg gaagagcaca gggaggccat cggcttccgg gagatcgaag tgaatgagca
     4561 ggtggccaag gtctccatcg tgggggccgg gatggtaaac aaccccggtg tggccgccag
     4621 gatgtttgag gccctctacg gtgccagcgt gaacatcaac atgatctcca ccagcgagat
     4681 caaggtctct gtcctggtga atgagcggga cgcagaccgg gcggtccagg ccatccacga
     4741 ccgcttcttc aacgaatttg gaggcaacgc ctgaacaggc gtcaaagcga tgcagccccc
     4801 ctcatggccc ggtcccgggc cgcgaggggg tgccctgttt tcaggttttc gcccgttttt
     4861 gccggaaaaa tcctttttta gcttgcaata tttgtgccgg tctggtaaaa taataactcg
     4921 actggatatt cttttcccat ccagtctgtt ttatcccccc gccgggaggc agacggaacg
     4981 ggatgcgggc tgactgaaaa cgagaatctc agggttgccg cccaagggcg cccctgctgc
     5041 aagtaaggag agaatcatcg caatggacaa gaatcagaga gaagagcggc gccgtcagga
     5101 ggatatcgca ctgaaccggg gcttgctct
//
LOCUS       DBMDSJ010000077         5103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4559_length_5103_cov_1.641943, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000077 DBMDSJ010000000
VERSION     DBMDSJ010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_5103_cov_1.641943"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="ACZPAQ_02940"
     CDS             complement(<1..602)
                     /locus_tag="ACZPAQ_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III domain-containing
                     protein"
                     /protein_id="HIR0902967.1"
                     /translation="MSTAITELFGTNVFSVSVMRERLPKKVFAEVTNVMENGGSISMS
                     TADVVANAMKNWALEKGATHYTHWFQPLTGITAEKHDSFLTAPVEGKTLLQLSGKQLI
                     QGEPDASSFPSGGLRSTHYARGYTAWDMTSPAFVKEMSCGTILCIPTIFVSYTGEALD
                     KKTPLLRSMEAINTQALRILRLFGNTEAHKVTPSVGPEQE"
     gene            1112..2443
                     /gene="glnA"
                     /locus_tag="ACZPAQ_02945"
     CDS             1112..2443
                     /gene="glnA"
                     /locus_tag="ACZPAQ_02945"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR0902968.1"
                     /translation="MSYTKEDIIRIVKEEDIEFIRMQFVDIFGQLKNVAITASQIEKA
                     VNNQITFDGSSIEGYTRINESDQYLYPDLNSFAIFPWRPAQGKVARLICDVYNPDGTP
                     FVGDPRGVLRRVLQRSRDMGFDAFNIGPELEFFLFETDDQGHATTLTRDEAGYFDLGP
                     LDHGESTRREICLNLEQMGFEIEASHHECARGQHEIDFKYDEALDCADKIMTFKLAVK
                     SLAQKNGLHATFMPKPLFGLAGSGMHINMSLFRDGKNIFFDEKGEKQLSSLAYSFIAG
                     LLEHVKGFTALTNPLVNSYKRLVPGYEAPCYCAWSASNRSALIRIPAARGQSTRVELR
                     SPDPSCNPYLAVAACLAAGLDGIQRGLTPPPEITGNIYEMDDSTRAASGIQALPSSLE
                     EALREMEADPVIMDVLGGHVASHYIHGKMKEWNEYCIRVSNWELEKYLVTY"
     gene            2480..3019
                     /locus_tag="ACZPAQ_02950"
     CDS             2480..3019
                     /locus_tag="ACZPAQ_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR0902969.1"
                     /translation="MDTVIVAFESDSLCRKFRDLLEGSGSAACLICHSGSQVRRAAAA
                     QPIYCVVCGTRLSDGPAEWLCGDLPPFCSLLMVGPAHQLALCSDPDIFKLPTPIRREE
                     ALSTVSLLLQFGHRMERYTRHRRSPLEQDLIARAKALLMERDGLTEEQAHRTLQKRSM
                     DQGLTLVHTARRTLAQGGG"
     gene            3102..3896
                     /gene="dapF"
                     /locus_tag="ACZPAQ_02955"
     CDS             3102..3896
                     /gene="dapF"
                     /locus_tag="ACZPAQ_02955"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR0902970.1"
                     /translation="MRFTKMEGLGNDYIYLNCLSRRPPDPAGLAVRLSDRHFGVGADG
                     LICVCPGRRGDFAMEMYNADGSRGKMCGNGIRCLGKYVYDKGLTRRTSLQVDTDSGLR
                     ALRLLPGPAGIGQVEVDMGEPSVFPQISIPLMELTYSVMPVSMGNPHCILFCPHPEEI
                     ALDVLGPALEHHPVFPDRTNVEFVAVEGDQLRLRVWERGSGETLACGTGACAALAAAH
                     FLGLCPPEVTALLPGGSLVLSLDTASGHIRMTGPAVTVFEGEWPGG"
     gene            3964..>5103
                     /locus_tag="ACZPAQ_02960"
     CDS             3964..>5103
                     /locus_tag="ACZPAQ_02960"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR0902971.1"
                     /translation="MAHINQNYLKLPGSYLFSEVNRRITAYSASHPGAKIIRLSIGDV
                     TRPLAPAVIEAMHQAVTEMGTFEGFHGYGPEQGYDFLREAIAQHDYAARGVDIKPEEI
                     FVSDGAKSDCGNIGDIFGLDNVVAVCDPVYPVYVDTNAMAGRAGDYQEELGRWNKLVY
                     MPCVEENGFTPVPPREKVDIIYLCFPNNPTGAVATREQLKAWVDYANANGSVILYDSA
                     YEAFICEEDVPHSIFEIEGARTCAIEFRSFSKTAGFTGNRCAYTVVPMELERDGAKLN
                     SLWNRRQCTKFNGVPYVVQRGAAAIYTPEGREQTRASIDYYRKNAQVIREGLTSAGLQ
                     VFGGVNAPYIWLKTPGGMGSWDFFDLLLDQANVATTPGAGFGPSGE"
BASE COUNT          905 a         1758 c         1498 g          942 t
ORIGIN      
        1 tactcctgct ccggccctac ggagggggtc accttgtggg cctcggtgtt tccgaacagc
       61 cgcaggatgc gcagggcctg ggtgttgatg gcctccatgg agcgcagcag gggagtcttt
      121 ttgtccagtg cctcgccggt gtaggagaca aagatggtgg ggatgcacag gatggtgccg
      181 caggacatct ccttcacaaa ggcgggggat gtcatgtccc aggcggtgta gccccgggcg
      241 tagtgggtgg accgcaggcc cccggagggg aaggaggagg cgtccggctc cccctggatc
      301 agctgcttgc ccgacagctg gagcagggtc ttgccctcca ccggggcggt gaggaaggag
      361 tcgtgcttct ccgcagtgat gccggtaagg ggctggaacc aatgagtata gtgggtagcc
      421 cctttctcca gcgcccaatt cttcatggcg ttggccacca catcggcggt gctcatgctg
      481 atgctgccgc cattctccat cacattggtc acttcggcaa acaccttttt gggcaggcgc
      541 tcccgcataa cggagacgct gaacacattt gtgccaaaca gctcggtgat cgcagtactc
      601 atttccagtc aaccctttcc atttccgtaa ttccaagccg ctcctgatct cccagccgca
      661 aaaacaaaaa agacaaagac atcctatcaa gacgtctttg tcttcattcg ttcccaaacg
      721 gaccggcgga ccggactgct ttctggttga cagcatactc ctttccggaa gtttttgcaa
      781 gtgttttttt catttccacc ataacgccca tttttttggg gccctctggg gcaggagttg
      841 cgaaaacgca caaatccctc tcccgtgaga tggcgccggc aggttcccgg agaccgggtg
      901 gccgcgcggg gccgccttcg cccgcaaagt gccccaagga agtccgcccc atccatttgc
      961 cggcccgccg cgccctgcgc ggcgggctcg gtactcgtcc tctccagcac tcccattcgg
     1021 aaaagtcttg acagccgccc cccttcagtg caatactgtt ttatccaaac attttccgtc
     1081 cctggggcgg tgagaaaggg gcagacacac catgagctat actaaggaag acattatccg
     1141 catcgtgaag gaggaggaca tcgaattcat tcggatgcag tttgtggaca tcttcggaca
     1201 gctgaagaat gtggccatca ccgcctccca aattgaaaag gcggtgaaca accagatcac
     1261 ctttgacggc tcctccatcg agggctacac ccgcatcaat gagtccgatc agtacctcta
     1321 ccccgatctg aacagctttg ccatcttccc ctggcggccc gcccagggca aggtggcccg
     1381 gctgatctgc gacgtgtata accctgacgg aacccccttt gtgggcgacc cccgcggggt
     1441 gctccgccgg gtgctccagc gcagccggga catgggcttt gatgccttca acatcgggcc
     1501 ggagctggag ttcttcctct ttgagactga cgaccagggc cacgccacca ccctcacccg
     1561 ggacgaggcg ggctacttcg acctgggccc cctggaccac ggtgagagca cccgccggga
     1621 gatctgcctg aatctggagc agatgggctt tgagatcgag gccagccacc atgagtgcgc
     1681 ccggggccag catgagatcg acttcaagta cgatgaagcc ctggactgtg cggacaagat
     1741 catgaccttc aagctggcgg tgaagagcct tgcccaaaag aacggactcc acgccacctt
     1801 catgcccaag cctctgttcg ggctggcagg ctccggcatg cacatcaaca tgtccctgtt
     1861 ccgggacgga aaaaatatct tttttgacga gaagggggaa aaacagctct cctccctggc
     1921 ctactccttt attgccggcc ttttggagca tgtgaagggc ttcaccgccc tcaccaaccc
     1981 cctggtcaac tcttacaagc ggctggtccc cggctacgag gctccctgct actgcgcctg
     2041 gtccgcctcc aaccgctccg ccctcatccg catccccgcc gcccggggcc agtccacccg
     2101 ggtggagctg cgcagccctg acccctcctg caacccctat ctggcggtgg ccgcctgcct
     2161 ggccgccggc ctggacggca tccagcgggg cctcacccct ccccctgaga tcaccggcaa
     2221 catctatgag atggacgaca gcacccgggc cgccagcggc atccaggccc tgccctcctc
     2281 cctggaggag gccctgcggg agatggaggc cgaccccgtc atcatggacg tgctgggcgg
     2341 ccatgtggcc tctcactaca tccacggcaa gatgaaggag tggaacgagt actgcatccg
     2401 ggtgtccaac tgggaactgg agaagtatct ggtcacctac tgaaccccgt ccccccgctg
     2461 aaaggagtga agccccccat tggatacggt catcgtcgcc tttgagagtg actccctctg
     2521 ccgcaaattc cgggatctgc tggagggctc cggctccgcc gcctgcctca tctgccactc
     2581 gggcagccag gtgcgccgcg ccgccgccgc ccagcccatc tactgcgtgg tctgcggcac
     2641 ccgtctgtcc gacggcccgg cggagtggct gtgcggggat ctgcccccct tctgctccct
     2701 gctgatggtg gggcccgccc accagctggc cctgtgttct gacccggaca tattcaagct
     2761 gccgaccccc atccgccgtg aggaggccct ttccaccgtc tccctgctgc tccagttcgg
     2821 acaccggatg gagcgctata cccgccaccg ccgctccccc ttagagcagg atctcatcgc
     2881 tcgtgccaag gccctgctca tggagcggga cgggctcacc gaggaacagg cccaccggac
     2941 cctccagaag cggagtatgg accagggcct cacactggtc cataccgccc ggcggaccct
     3001 cgcccagggc ggaggctgag ctgcggcata ttcccgcctg ccggcgcata tccatcctcc
     3061 agaccgggcg cgcccgccgc gccccgaaaa ggagtgatgg catgcgcttc accaaaatgg
     3121 aggggctggg aaacgactac atctacctca actgcctctc ccgccgtccc cccgaccccg
     3181 ccgggctggc ggtccggctc tccgaccgcc actttggtgt gggggcggat ggcttgatct
     3241 gcgtctgccc cggccggcgg ggggactttg ccatggagat gtacaacgcc gatggctccc
     3301 ggggaaagat gtgcggcaac ggcatccgct gcctgggcaa gtatgtgtac gacaaggggc
     3361 tcacccgccg caccagcctc caggtggaca ccgactccgg cctgcgtgcc ctccggctcc
     3421 tccccggccc cgccggcatc ggacaggtgg aggtggacat gggggagccc tccgtctttc
     3481 cccagatctc catccctctg atggagctca cctactccgt catgcctgtc tctatgggca
     3541 acccccactg catcctcttc tgcccccacc cggaggagat cgccctggac gtgctgggcc
     3601 ccgccctgga acaccacccc gtctttcccg accggaccaa cgtggaattt gtggccgtgg
     3661 agggggatca gctgcggctg cgggtgtggg agcggggcag cggcgagact ctggcctgcg
     3721 gcaccggcgc ctgcgccgcc ctggctgccg cccacttcct gggactgtgc cccccagagg
     3781 tcaccgccct tctccccgga ggctccctgg tcctgtctct ggacacagcc agcgggcaca
     3841 tccgcatgac cggccccgcc gtcaccgtgt tcgaggggga gtggcccgga ggttaggatt
     3901 gacagcgggg caaaattccg ccataatagt ttgtaagttt ttctgcaaag gaggggtctc
     3961 cccatggccc acatcaacca aaactatctg aagctgcccg gcagctacct cttctccgag
     4021 gtcaaccgcc ggatcaccgc ctactccgcc tcccaccccg gcgccaagat catccggctg
     4081 tccatcggcg acgtgacccg ccccctggcc cctgctgtca tcgaggctat gcaccaggcc
     4141 gtcaccgaga tgggcacctt cgagggcttc cacggctatg gcccggagca gggctacgac
     4201 ttcctgcggg aggccattgc ccagcacgac tacgccgccc ggggcgtgga catcaagccg
     4261 gaggagatct ttgtctccga cggcgccaag agcgactgcg gcaacattgg cgacatcttc
     4321 ggtctggaca acgtggtggc ggtgtgcgac ccggtgtacc ctgtctatgt cgataccaac
     4381 gccatggccg gccgggccgg tgactaccag gaggagctgg gccgatggaa caagctggtc
     4441 tacatgccct gtgtggagga gaacggcttt acccctgttc cccctcgtga gaaggtggac
     4501 atcatctacc tctgcttccc caacaacccc accggcgcgg tagccacccg ggagcagctg
     4561 aaggcgtggg tggactacgc caacgccaac ggctccgtca tcctctacga ctccgcctat
     4621 gaggctttca tctgcgagga ggatgtcccc cacagcatct ttgagatcga gggtgcccga
     4681 acctgtgcca tcgagttccg ctccttctcc aagactgccg gcttcaccgg caaccgctgc
     4741 gcctacaccg tggtccccat ggagctggag cgggacggtg ccaagctgaa cagcctgtgg
     4801 aaccgccgcc agtgcaccaa gttcaacggg gtcccctatg tggtccagcg gggcgccgcc
     4861 gccatctaca cccccgaggg cagggagcag acccgcgcct ccatcgacta ctaccgcaaa
     4921 aacgcccagg tcatccggga gggcctcacc tctgccgggc tccaggtgtt cggcggggtc
     4981 aacgctccct atatctggct caagaccccc ggcggtatgg ggtcctggga cttctttgat
     5041 ctgctgctgg atcaggccaa tgtggccacc acccccggcg ccggcttcgg ccccagcggt
     5101 gag
//
LOCUS       DBMDSJ010000078         5062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4595_length_5062_cov_1.587583, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000078 DBMDSJ010000000
VERSION     DBMDSJ010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_5062_cov_1.587583"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="ACZPAQ_02965"
     CDS             <1..956
                     /locus_tag="ACZPAQ_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HIR0902972.1"
                     /translation="PYNFYALLTIVMMLGLVLMKVDFGPMAKFERNAIENNDLFSGSN
                     PYAMLDEEIDDTKGTVMDLVLPIAVLVVCCVIGMIYSGGFFSGTDFVSAFSNSDASVG
                     LMFGSAFGLLFALIYYTVRRSMSFKEMMSCVPEGFKAMVPAILILTFAWSLKSMTDSL
                     GAKYFVRDFVYNNAESLQNFLPAIVFLIGCGLAFATGTSWGTFGILIPIVQNVFPLDN
                     PLGIICISACMAGAVCGDHCSPISDTTIMASAGAQCDHVNHVSTQLPYAISCAVISFV
                     TYIVAGFVQTPFIALPVGIVLMLAFLFIMKQLSPGQSHRAA"
     gene            complement(1044..1172)
                     /locus_tag="ACZPAQ_02970"
     CDS             complement(1044..1172)
                     /locus_tag="ACZPAQ_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902973.1"
                     /translation="MTTQEAIRELQNIRQYCAAKSIPAVDFALKVLREKLEQEEKQ"
     gene            complement(1426..2076)
                     /locus_tag="ACZPAQ_02975"
     CDS             complement(1426..2076)
                     /locus_tag="ACZPAQ_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR0902974.1"
                     /translation="MTGLMMKDALVMRKTLRLYALFLLFYSGLAVLGVFPMSMALAMV
                     EVIVMVLPISSFSYDEAAKWDRYAAVLPVGRTAVVKARYLFLLLVLLAAAAIGLVGSI
                     LASLLAREDLGELLATLLVSMGLGLMIADIMLPVVYQLGPERARPWMYVIIFAPIVVL
                     LGGAKLGLFDGIDLSWLDQLPELAVLGVFALVPLVPLVGMLLSCWISCRIYARKEF"
     gene            complement(2073..2933)
                     /locus_tag="ACZPAQ_02980"
     CDS             complement(2073..2933)
                     /locus_tag="ACZPAQ_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0902975.1"
                     /translation="MENIIVAEGLRKEYDGFALEDVSFRVPGGAIMGLIGENGAGKTT
                     TIKCLLNLVRRDAGTVTLLGMDDLEGEKEIKQDVGVVLDECFFHDSLRAKDVGVILAP
                     VYRGWDEELYRRYLKKYKLPEKKFIKEYSRGMKMKLSLAAALAHRPRLLILDEATAGL
                     DPVVRDEILDEFQGFIEDEEHAILISSHITSDLEKIADYITYIHQGRVVLSDSKDAIL
                     EHYAKLGCTQAQLRDIRPGDLVRVRKGSFGCEALVRDRAAFARKYPGLMLDRVTLEDI
                     MLLIGKGESA"
     gene            complement(2938..3312)
                     /locus_tag="ACZPAQ_02985"
     CDS             complement(2938..3312)
                     /locus_tag="ACZPAQ_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0902976.1"
                     /translation="MDIIISNSSGKPIYEQIADQVKEQIMAGALAAGDALPSMRLLAK
                     ELRISVITTKRAYEELERDGFLENVPGKGCFVAPQNRELLREAQLRRVEEKLTQAIEE
                     ARRGAVSLEELKEMLTELYQEV"
     gene            complement(3654..4499)
                     /locus_tag="ACZPAQ_02990"
     CDS             complement(3654..4499)
                     /locus_tag="ACZPAQ_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR0902977.1"
                     /translation="MSVQILTDSTSDILPEEAEQKGVRVVPLLVNFGEEAFRENVEIT
                     HQEYFRRLAQAESLPTTSQPTPQDFLAHFEQVRDAGDSLVCVLLASKLSGTFQSAQLA
                     KELSGYDEIYIVDSQQALCGLRMLVDFACVLRDEGLSAREIAEQVESASHRVRLFGIV
                     NTLEYLHKGGRLPASMAVVGTLLKVKPLITLREGEVGILGKGLGVKNGLDNLLKLMGD
                     EIHPEPRLPLYFGYTQTHNLCDQLRERVVEKYHPARVEQNSVGAVVGTHVGPDGAVVV
                     YLDQE"
     gene            complement(4502..4996)
                     /locus_tag="ACZPAQ_02995"
     CDS             complement(4502..4996)
                     /locus_tag="ACZPAQ_02995"
                     /inference="COORDINATES: protein motif:HMM:NF047172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0902978.1"
                     /translation="MTAEQERYFKSFLDHMFDHIMDLEERALHHVGADDLSVRELHVL
                     DAVGERMADGRNTMTGIADGLSIQVSSLTVAVNTLVRKGYLVRERENRDRRVIRVLLT
                     EKGAEASRVHTQFYGRVVHRVCSRLNEEESQVLVQCLRQLDELFDSLKRPGKTGVSEK
                     KGGS"
BASE COUNT          901 a         1781 c         1331 g         1049 t
ORIGIN      
        1 tcccctataa cttctatgcc ctgctcacca tcgtaatgat gctgggtctg gtcctgatga
       61 aggtggattt cggccccatg gccaagttcg agcgcaacgc cattgagaac aacgacctct
      121 tctccggttc caacccctat gccatgctgg atgaggagat cgacgacacc aagggcacgg
      181 tcatggacct ggtgctgccc atcgcggtgc tggtcgtctg ctgcgtcatc ggcatgatct
      241 actccggcgg cttcttctcc ggcaccgact ttgtctctgc cttctccaat tcggacgcct
      301 ccgtgggcct gatgttcggc tctgccttcg gcctgctctt cgccctgatc tactacaccg
      361 tgcgccggtc catgtccttc aaggagatga tgtcctgcgt tcctgagggc ttcaaggcca
      421 tggtccccgc catcctcatc ctcacctttg cctggtctct gaagtccatg accgactccc
      481 tgggcgccaa atattttgtc cgtgattttg tgtacaacaa tgcggagagc ctgcagaact
      541 tcctgcccgc cattgtgttc ctcatcggct gcggcctggc ctttgccacc ggcaccagct
      601 ggggcacctt cggcatcctg atccccatcg tgcagaacgt cttccccctg gacaatcccc
      661 tgggcatcat ctgcatctcc gcctgcatgg cgggcgctgt gtgcggcgac cactgctccc
      721 ccatctctga caccaccatc atggcctctg ccggcgctca gtgcgaccat gtgaaccacg
      781 tgtccacgca gcttccctac gccatctcct gtgcggtcat ctcctttgtc acttatattg
      841 tggccggttt cgtgcagacc cccttcattg ccctgcctgt gggcatcgta ctcatgctgg
      901 ctttcctgtt tatcatgaag cagctctccc ccggccagtc ccaccgcgcc gcatgaccca
      961 gctcttttcc ggccatctta tttccctcaa gtgaaaagcc ccggcatccg cattttcgga
     1021 tgccggggct ttttcctgtc tttttactgt ttttcctcct gctccagctt ctcccgaagc
     1081 actttgaggg caaaatccac cgctgggatg gacttggccg cgcagtactg gcggatattc
     1141 tgcagctctc ggatcgcctc ctgtgtcgtc acggcgctca cgctcccttt ccctgtattc
     1201 tccttttatt gtaccacggg gtccgggccg ggcgcaaggg gcccgccgtt ccggcgcgct
     1261 cattttccgc cccggccccc gccccagagg atcaggagga cacccccggc cgctattccc
     1321 caggccgccg gaccgtccgg ctcaatgctc ctggtaagac agaggagagc cagcgccagc
     1381 cccagcgcct ttccgcccca cagggacgcc cgcgcccagt ccacctcaga actccttcct
     1441 ggcgtagatc ctgcaggaga tccagcagga caggagcatc cccaccaggg gcaccagtgg
     1501 caccagggca aagaccccca ggacagcaag ctcgggcagc tgatccagcc aggacaggtc
     1561 tatcccatcg aagaggccca gcttggcgcc ccccagcagc accacgatag gggcaaaaat
     1621 gatgacatac atccaaggcc gggcccgctc cggccccagc tgatagacca ccggcagcat
     1681 aatgtccgcg atcatcagcc ctaagcccat agagaccagc agggtggcca gcagctcccc
     1741 cagatcctcc cgtgccagca gggaggccag gatgctcccc accagcccga tggccgccgc
     1801 cgccagcagc accagcagca ggaagagata ccgggccttt accacagcgg tacgtcccac
     1861 gggcagcaca gctgcgtacc ggtcccactt cgccgcctcg tcatagctga agctggagat
     1921 gggcagcacc atcacgatca cctccaccat agccagggcc atggacatgg ggaaaacgcc
     1981 cagcacggcc aatccggagt agaacaacag gaacagggca tagagcctca gggtcttccg
     2041 catcacaagg gcatccttca tcatcaggcc ggtcatgccg actccccctt tccgatcaag
     2101 agcatgatat cctccagcgt cacccggtcc agcatcaggc cggggtattt ccgggcaaag
     2161 gccgcccggt cccgtaccag cgcttcacag ccgaagctgc ccttccgcac ccgcaccaga
     2221 tctccgggcc ggatgtcccg caactgggcc tgggtgcagc ccagcttggc atagtgctcc
     2281 agaatggcgt ccttgctgtc cgagagcacc acgcggccct ggtggatgta ggtgatatag
     2341 tctgcgatct tctccaggtc gctggtgata tggctggaaa taaggatcgc gtgctcctcg
     2401 tcctcgataa agccctggaa ctcgtccagg atctcatccc gcaccaccgg gtccagcccc
     2461 gcggtagcct cgtccaggat cagcagccgg ggccggtggg ccagggccgc cgccagggac
     2521 agcttcattt tcatgccccg ggagtattcc ttgataaatt ttttctccgg cagtttgtat
     2581 ttcttcagat accggcggta gagctcctcg tcccaccccc ggtacaccgg cgccagaatg
     2641 acgcctacgt cctttgcccg caggctgtca tggaagaagc actcgtccag cactactccc
     2701 acatcctgct tgatctcctt ctctccctcc agatcatcca tccccagcag ggtgacggtc
     2761 ccggcgtccc gccggaccag gttcagcagg catttgatgg tggtggtctt gcccgccccg
     2821 ttttccccga tcagtcccat gatggctccg ccggggaccc ggaaggagac atcctccagg
     2881 gcaaatccgt cgtattcctt ccgcagtccc tctgcgacaa tgatgttttc catcgcttta
     2941 cacctcttga tacagctctg tcagcatttc cttcagctcc tccagagaca ccgccccgcg
     3001 tcgggcctct tcgatggcct gggtgagctt ttcctccacc cggcgcagct gggcctcccg
     3061 cagcagctcc cggttctggg gggctacaaa gcaccccttt cccggcacat tctccaggaa
     3121 tccgtcccgc tccagctcct cataggcccg cttggtggtg atgacggaga tccgcagctc
     3181 cttggccagc aggcgcatgg aaggcagggc gtcccctgcc gccagcgcgc ctgccatgat
     3241 ctgctccttg acctggtccg cgatctgttc atagatcggc tttccgctgg aattactgat
     3301 gatgatgtcc acttgcggca ccttataagc accccgccag accaggcgtg cggcgtccgg
     3361 accgccgccg gcccgcccag gggagcgctt atcctttctc tcgggtcata ttgtatatat
     3421 actatatgca cagtataaca gtcattttcc ttttgtcaat aggccggccc tatttttttg
     3481 aaaaaattgt gggccgcccg aaaggccttc tctcaggaaa ggcggcacgg cgcataccgc
     3541 cataaatgag ggggcggcct tccatggaga tgcaggcctg cagggaacag cgaaagccgc
     3601 cccctccgcc aagctggcga aaggggcggc tttttccgca atcccgcaaa atattattcc
     3661 tgatccaggt agaccactac ggctccgtcg gggcccacat gggtacccac cacagccccc
     3721 acagaattct gctccacccg ggccgggtga tacttctcca ccacccgctc ccgcagctgg
     3781 tcacagaggt tgtgggtctg ggtatatcca aagtacaggg gcagacgggg ctcaggatgg
     3841 atctcgtccc ccatcagctt cagcaggttg tccaggccgt tcttgacccc caggcccttg
     3901 cccaggatgc ccacctctcc ctcccgcagg gtgatgaggg gcttcacctt cagcagcgtc
     3961 cccaccaccg ccatggaggc gggcaggcgt ccccccttat gcagatactc cagggtgttc
     4021 acgatgccga acaggcgcac ccggtggctg gcggattcca cctgctccgc gatctcccgt
     4081 gcggacagcc cctcatcccg cagcacgcag gcaaagtcta ccagcattcg cagcccgcac
     4141 agggcctgct gggagtccac gatatagatc tcgtcgtagc cgctgagctc cttggccagc
     4201 tgagcgctct ggaaagtgcc gctgagcttg gaagccagca gcacacacac caggctgtcc
     4261 cccgcgtccc gtacctgctc aaaatgggcc aggaagtcct ggggggtggg ctggctggtg
     4321 gtcggcaggc tctccgcctg ggccagccgt cgaaaatact cctggtgggt gatctccaca
     4381 ttctcccgga aggcctcctc cccgaagttc accagcagcg ggaccactcg cacccccttc
     4441 tgctccgcct cttccggcag gatatcggat gtgctgtcgg tcaggatctg aacggacatg
     4501 gtcaagaacc tccctttttt tcactgacgc cggtcttccc cggccgcttc aacgagtcga
     4561 acagctcgtc cagctgacgc aggcactgga ccagcacctg ggactcctcc tcattcagac
     4621 ggctgcatac ccggtggact acccggccat agaactgggt atggacccgg ctggcctccg
     4681 cccccttctc agtcagcagg acccgtatca cccgccggtc ccggttctcc ctctcccgga
     4741 ccaggtatcc cttccgcacc agagtgttca ccgctaccgt cagcgagctg acctggatgg
     4801 agaggccgtc ggcgatgcct gtcatggtgt tgcggccgtc cgccatccgc tcccccactg
     4861 catccagcac gtgcagttcc cgcacggaga ggtcatccgc ccccacgtgg tgcagggccc
     4921 gctcctccag atccatgata tggtcgaaca tgtggtccaa aaagctcttg aaatagcgct
     4981 cctgctccgc tgtcaccggg ctctccccct ctccgccggc aagtattttt ctttgaattt
     5041 caaatagttt gaagttctaa tt
//
LOCUS       DBMDSJ010000079         5053 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4607_length_5053_cov_1.614499, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000079 DBMDSJ010000000
VERSION     DBMDSJ010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_5053_cov_1.614499"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            93..2231
                     /locus_tag="ACZPAQ_03000"
     CDS             93..2231
                     /locus_tag="ACZPAQ_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0902979.1"
                     /translation="MTRFSQVTAALRRKSLGQYALLTLCCFVSVLLITAYASMMGSPT
                     VLSVFPEGGDSRKQMTMIFVLAVLGCGVFTTYASGLFFRHKSRDVGILLALGASKDQL
                     RRVLAGELALMSLSACALGALLGTPLAWFIWQGFRLLLVDSEEMALAFDPHAYLLALA
                     FSLFVVVMLGVMLGRFLRRTNILDVVNESRKSEPIRAVPRWYGPLGIILLAAGGFLGY
                     MAPKFFILVFHWYAPDALTALFYLPALAGLYMILLHTVVNGWRRGSSRYRDLITSSMM
                     QFQGRQTVRNMVVVTVLAAGAYFAAFYIPMLSSGTSSTTEDREFDYLFHYRADQDMPG
                     REEVLHLAEEMGVTVTRYAQQSGAVLAYDGYADVVSEGPLGATYDVVYCEETASDVFL
                     SESAWNILTGDDLDLEPGTVATVYDRNGDSSGAGNSISLVTNHLTGEQLAVTPAEPLR
                     STSLLGYRVLDDGDYARITQGLPDEWREVVAVFDVEDEEDTYFFARALFDEIVDRSGP
                     EVALFDGWDPVVRDRYLAEKGYYFLDPAHLEPNGFSPIEYDQRDSSNFRNSWKYMPRF
                     RVLDQADFTTTMAVFLMLFVFVSIVCFAAMVVILFTRCMTIAMTNARVYDDLRHLGAS
                     SAFLRRTVKGQISRVFFVPLCTGTLLIYAFFAMILYFNSGSFTPGELAGLANCALVVL
                     VLSLFLYGMYRFTLGRVCSALSIRSSSRPR"
     gene            2443..4644
                     /locus_tag="ACZPAQ_03005"
     CDS             2443..4644
                     /locus_tag="ACZPAQ_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902980.1"
                     /translation="MRKRALSLLLAMALVLGLSVGASAASAAPTDLNRYLLSHTSRSG
                     ETRSLPIDRTEVNEHPVIGYYTYTFQDGEAAGRSFKLYVGQHAALRAYITVIALPDGI
                     ADPYAFLQEQGWLEQADTYGELLFVLEPGQGGWGTPDSEAAYLEACLGEVIGNDAGGT
                     RETAPGGVVQTGKVTLSDGTTCSFFTGHSCNYYVGYGEGCAVLESWTAENPLYVISQA
                     FIGGKSVGSGYLDQAAARTYNGYNVSTYHPGYSDEDFTATLTDMKNDGAIASAKFMTN
                     ADVPVPTLFAGYPQGDASVSYWKNVNDAYAVPTVDGLYRQSISSSSWSTRYANQNAKT
                     WDPNTRYGISQVRVEDGADLNAAQIRDFLAAYTRYTYQFAYSNNLAYRLDYYQATKAA
                     RTAAESGKAIATYTFDGYDGSDATVELRALESARVSVPGSLVGGTVYSCISAFNDYDS
                     NGVLDPRESLIYIPDSVKNNSAEGAPVLVVFPGMTQAASTFMDCSGYWAVANDEGCVL
                     LIMGEYCNSSASSLIYGDEEDNVNMTRSALLLLDQVVSKQAGVQMDLTRVYGTGHSLG
                     SRTVQTLTHNSEAYYFAAVASTSFNNTQFTADSMMPSYLLAGQADIDSLVAAPWLSGD
                     NNSIHDWVTGALKMNGLDVTFTPNDHASFLAACSSYDESGRYYTYTWADSNSIPLVQF
                     TRTLAREHNCYPEEFRLAWDFLEHYRLAEDGTRYYSPSAFANNDAVTITKG"
     gene            4811..>5053
                     /locus_tag="ACZPAQ_03010"
     CDS             4811..>5053
                     /locus_tag="ACZPAQ_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR0902981.1"
                     /translation="MGRLFSRRKYRDLMLGLALLCAVLALMRWPQESMAAARDGLALC
                     GNVIIPSLFPFFVLSSLVVELGMSRYLGRLLEGVMAQ"
BASE COUNT          854 a         1739 c         1441 g         1019 t
ORIGIN      
        1 tggcgcaccc tggcccgcgg cgagaaggac cgacgcacct tccaggacga gctgctggac
       61 gcggtgcggg agatggggga ggcgagcgcc caatgacacg tttctcccaa gtgaccgccg
      121 ccctgcggcg gaagagcctg gggcagtatg ccctgctcac cctgtgctgt ttcgtctccg
      181 tgctcctcat caccgcctac gcctccatga tgggttcccc cacggtgctc tccgtctttc
      241 ccgagggggg cgacagccgc aagcagatga ccatgatctt cgtgctggct gtcctgggct
      301 gcggcgtatt caccacctac gcctccggcc tgttcttccg ccacaagtcc cgtgatgtgg
      361 gcatcctgct ggccctgggt gcctccaagg accagctgcg ccgggtgctg gcgggggagc
      421 tggctctgat gtccctgtcc gcctgtgccc tgggcgccct gctgggcacc ccgctggcct
      481 ggttcatctg gcaggggttc cggctcctgc tggtggactc agaggagatg gccctggcct
      541 ttgaccccca tgcctatctg ctggccctgg ccttctccct ctttgtggtt gtcatgctgg
      601 gcgttatgct gggccgcttt ttgcggcgga ccaacatcct ggacgtggtc aacgagtccc
      661 ggaagtctga gcccatccgt gcggtccccc ggtggtacgg tcctctgggc atcatcctgc
      721 tggcggcggg cggctttctg ggctatatgg cccccaaatt ttttattttg gtcttccact
      781 ggtacgcccc cgacgccctg acggccctgt tctatctgcc cgccctggcc ggcctctaca
      841 tgatcctcct ccacacggta gtcaacggct ggcggcgggg cagcagccgc tacagggacc
      901 tgatcacctc cagcatgatg cagttccagg gacgccagac cgtgcggaat atggtggtgg
      961 tgacggtgct ggccgccggg gcctactttg ccgctttcta tatccctatg ctgagcagcg
     1021 gcacctcgtc cacaacggag gaccgggaat tcgattacct gttccactac cgggctgacc
     1081 aggacatgcc cggccgggag gaggtgctcc atctggcaga ggagatggga gtgactgtca
     1141 cccgctacgc ccagcagtcc ggtgcggtgc tcgcctacga cggctacgcc gatgttgtat
     1201 cggagggccc cctgggcgcc acctacgatg tggtctactg tgaggaaact gcctctgatg
     1261 tgttcctgtc ggagagcgcc tggaacatcc ttaccgggga tgacctggat ctggaacccg
     1321 gtacagtggc cacagtctat gaccgcaacg gggatagcag cggcgccggg aacagcatct
     1381 ccctggtcac caaccacctc accggggaac agctggccgt cacgccggcc gagcccctgc
     1441 gcagcacctc ccttctggga taccgggtgc tggacgacgg ggattatgcc cgcatcaccc
     1501 agggcctgcc cgatgaatgg cgggaggtgg tggccgtctt tgacgtggag gacgaggagg
     1561 acacctactt ctttgcaagg gccctgttcg acgagatcgt agaccgctcc ggccccgagg
     1621 tggccctctt cgacggttgg gacccggtgg tccgggaccg ttatctggca gagaagggct
     1681 actacttcct ggacccggcc catctggaac ccaatggatt ttctcccatt gaatatgacc
     1741 agcgggattc cagcaacttc cggaattcct ggaaatacat gccccgcttc cgggtgctgg
     1801 accaggcgga cttcaccact accatggcgg tctttctgat gctgttcgtc tttgtctcca
     1861 tcgtctgctt tgccgctatg gtggtcatcc tctttacccg ctgcatgacc atcgccatga
     1921 ccaacgcccg ggtctatgac gacctgcggc atctgggggc ctccagcgcc ttcctccgcc
     1981 gcacggtaaa ggggcagatc tcccgggtgt tcttcgtccc cctgtgcacg ggcaccctgc
     2041 tgatctacgc cttcttcgcc atgatcctgt acttcaacag cggcagcttc actcccggcg
     2101 agctggccgg cctggccaac tgcgccctgg tggtcctcgt cctctctctg ttcttatatg
     2161 gcatgtaccg cttcaccctg ggccgggtat gctccgccct ttccatccgg tcctcttccc
     2221 ggccccgctg acctccttcc cctctctcat cccatcccat gcatttttcc catccattta
     2281 tcaaaatttc tcactggaaa gcggcagtca aaacagctat aataaagacg aaagcgcagg
     2341 aaaaattgta aattttgtct aattgctgtc tttagacccc gccgacagcg atgcccgcgc
     2401 ccagcacgca ccaacaaacg agggaatgag gaggaaatca tcatgcgcaa acgagctttg
     2461 tccctgcttc tggcaatggc cctggtcctg ggcctgtctg tgggcgcctc cgcggcctcc
     2521 gccgcgccca ccgatctcaa ccggtatctg ctctcccaca ccagcaggag cggcgagacc
     2581 cgctccctgc ccatcgaccg cactgaagtc aatgagcacc ccgtcatcgg ctattacacc
     2641 tacaccttcc aggacggcga ggccgccggc cgctccttca agctctatgt gggccagcac
     2701 gccgccctcc gcgcctacat caccgtcata gccctgcccg acggcattgc ggacccctac
     2761 gccttccttc aggagcaagg ctggctggag caggccgaca cctatggcga gctcctcttt
     2821 gtcctggagc ccggccaggg cggctggggc acccctgaca gcgaggccgc ctatctggag
     2881 gcctgcctgg gcgaggtgat cggcaacgac gccggcggca cccgtgagac cgcccccggc
     2941 ggcgtggtcc agaccggcaa ggtgaccctc agcgacggca ccacctgttc cttcttcacc
     3001 ggccactcct gcaactacta tgtaggctac ggcgagggct gcgcggtgct ggagtcctgg
     3061 accgccgaga accccctgta cgtcatcagc caggccttca tcggcggcaa gagcgtgggc
     3121 tccggctatc tggaccaggc cgctgcccgc acctataacg gctataacgt gagcacctat
     3181 caccccggct atagcgatga ggacttcact gccaccctga ctgacatgaa gaacgacggt
     3241 gccatcgcct ccgcgaagtt catgaccaat gcggatgtcc ctgtgcccac cctcttcgcc
     3301 ggctatcccc agggggatgc cagcgtcagc tactggaaga acgtcaatga cgcctatgct
     3361 gtccccacag tggatgggct gtatcgccaa agcatctcct ccagctcctg gagcacccgc
     3421 tatgccaacc agaacgccaa gacgtgggac cccaataccc gctacggcat ctctcaggtc
     3481 cgggtggagg acggcgctga cctgaacgcc gcccagatcc gggacttcct ggccgcctac
     3541 acccgctaca cctatcaatt tgcttacagc aacaacctgg cctaccgtct ggactactat
     3601 caggccacca aggctgcccg gactgccgcg gagagcggca aggccatcgc cacctatacc
     3661 ttcgatggct atgacggcag cgatgccact gtggagctgc gggccctgga gtccgcccgg
     3721 gtctccgtcc ccggtagctt ggtgggcggt acagtgtact cctgcatctc cgctttcaac
     3781 gactatgaca gcaacggcgt cctggacccc cgcgagtctc tgatttacat ccccgactct
     3841 gtgaaaaaca acagtgctga gggtgctcct gtcctggtgg tcttccccgg tatgactcag
     3901 gccgcctcta cctttatgga ctgctccggc tactgggccg tggccaacga cgagggctgc
     3961 gtcctgctca tcatgggcga gtattgcaac tccagcgcct ccagcctgat ttatggcgat
     4021 gaagaggaca acgtcaacat gacccgctcc gccctgctgc tgctggacca ggtggtcagc
     4081 aagcaggctg gtgtccagat ggacctcacc cgggtgtacg gcaccggcca ttccttgggc
     4141 agccggacgg tccagaccct gacccacaac agcgaggcct actacttcgc cgctgtggcc
     4201 tccacctcct tcaacaacac ccagtttacc gctgacagca tgatgcccag ctatctgctg
     4261 gccggtcagg ccgacatcga cagcttggtg gccgctccct ggctctccgg tgataacaat
     4321 tccatccatg actgggtcac cggcgcgctg aagatgaacg gcctggatgt gaccttcacc
     4381 cccaacgacc acgccagctt cctggccgcc tgctccagct atgacgagag cggccgctac
     4441 tacacctaca cctgggcgga cagcaacagc atccccctgg tccagttcac ccgcaccctg
     4501 gcccgggagc acaactgcta ccccgaggag ttccggctgg cctgggactt cctggagcac
     4561 taccgcctgg ccgaggacgg cacccgctat tacagcccct ccgcctttgc caacaacgac
     4621 gctgtgacca tcactaaggg ctgaagcccc ttcttccaac tgaactgccc cggcgcggtc
     4681 ctgagaccgc gccggggcag ttttctctcc tgtttcagaa aaagggcatc ttttttctgc
     4741 ccgcgcatat agatccataa gggaccgcgc acgttccgtg cggcccgctc tgcgccggga
     4801 ggtgagcccc atgggccgtc tgttctccag aagaaaatac cgggacctga tgctgggcct
     4861 ggctctgctg tgcgctgttc tggccctgat gcgctggccc caggagagta tggccgccgc
     4921 ccgggacggg ctggccctgt gcggcaatgt gatcatcccc tccctttttc ccttctttgt
     4981 cctctcctcc ctggtggtag agctggggat gagccgctac ctgggccgtc tgctggaggg
     5041 ggtcatggcc cag
//
LOCUS       DBMDSJ010000080         5026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4635_length_5026_cov_1.665990, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000080 DBMDSJ010000000
VERSION     DBMDSJ010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4635_length_5026_cov_1.665990"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="ACZPAQ_03015"
     CDS             <1..358
                     /locus_tag="ACZPAQ_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR0902982.1"
                     /translation="LGAPDMRLPIQYALTWPKRTPGPAVPLDLWNCGPLTFGTPDLDT
                     FRCLPLALEAARTGGTAGAILNGANEAAVARFLEGKIGFLDIAEQVARAMDQVPVVQR
                     PTLEDILEADRAARAV"
     gene            394..1473
                     /locus_tag="ACZPAQ_03020"
     CDS             394..1473
                     /locus_tag="ACZPAQ_03020"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR0902983.1"
                     /translation="MLGSVLFYVVIAVLIFGVLIATHELGHFATAKLLGVQVNEFAVG
                     MGPALWKKEKGETLYSIRALPIGGYCAMEGEDEESDNPRAFGRASGWKKIVILCAGAF
                     MNFLTGFVLILILFSQATGFVLPTIDGFLEGYGVEDSGLQPGDVVYSLDGHRIYNYNN
                     LTFFLGRAGDTLDFVVLREGEKVRLDGVEMPLQARTDEEGKTTYLRGISVGREIIPAT
                     LGSKLLYSWYNSLDFVRTVWISLGDLVSGVVGLRDLSGPVGIVTTISQVGTQSETVGD
                     AVYNILYLSALIAVNLAVMNLLPLPALDGGRIFFLFLNGLLFGLFRRKIDPKYEGYVH
                     MAGLALLLILSLAVTFSDVGKLFGV"
     gene            1506..2543
                     /gene="ispG"
                     /locus_tag="ACZPAQ_03025"
                     /gene_synonym="gcpE"
     CDS             1506..2543
                     /gene="ispG"
                     /locus_tag="ACZPAQ_03025"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR0902984.1"
                     /translation="MTRQIHVGGIPIGGGAPVTIQSMTNTRTDDVESTLSQIRSLAAA
                     GCQIVRVAVPDGAAARAVAKIKEASPLPVVVDIHFDYKLALEAIAAGADKVRINPGNI
                     GGEDRVKAVADACRLRGIPIRIGVNGGSLEKGLLAKYGGVCPEAMVESAFGHIRLLNK
                     FDFDDICVSLKSSSVPMTMRAYQLMREKSNYPLHIGVTEAGTVRMGTLKSAVGIGGLL
                     ALGIGDTMRVSLSADPVEEVYAARDILKAAGVRREGAELVSCPTCGRTRIDLIGLAAQ
                     VEERLKGVDKPITVAVMGCAVNGPGEASAADCGIAGGIGEGLLFRKGEIVKKVPQDRL
                     VDELFALIEEL"
     gene            2603..>5026
                     /locus_tag="ACZPAQ_03030"
     CDS             2603..>5026
                     /locus_tag="ACZPAQ_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR0902985.1"
                     /translation="MSKKIPFLQMFAALCQWQELAEAVEGWVIVAAAIDKASRSALVT
                     LDGARGAGANLIRQAEEAVARCYGLASVKFQMAPPEPETPAEAPAPAAPSVPASAPQT
                     RPAHPRQGESTAPEMDAFARTEAIRREALRKARGSAPAARPKGKGEGKPSGKTIFGHV
                     IKKRPTPIGELELDMGMVVIEGDVFAVDNRELKKRGAWVVAFDLTDYTGSIRVNKFFP
                     GDEGKPIVDGVKKGMHLQIQGRLNMDRFYGDMVLEPMSIMAVPKPMKMDDAPEKRVEL
                     HLHTTMSAMDALTSVGPKLGPDKNVVKRAEAWGHRAIAITDHGVAQSFPDAWHSAKNI
                     KILYGVEAYYINDVDDRVVVHGETEQPFDQEIVCFDIETTGLNRKYEVIIEIGAVVLK
                     NGEITDRFNTFVSPGRILSPEIIRLTGITDEMLVGAPSQEEALRAFLAFAGDRPLAAH
                     NADFDMGFIAAGCRKYGIPFHNPSIDSLILAQNLLPDLGKYKLDIVAEHLHLPAFNHH
                     RASDDAATVGYMLPPFFKMLEEMGLRHLGEINGAMVHLRKGGKAKRQPKHLIVLARNQ
                     TGLRNLYKLISLGHLDYFKRYPIMLKSVINENREGLILGSACEAGELFRAVADGKDWE
                     ELKRIASWYDYLEIQPLCNNMFMLRKGMVRTEEELRDFNRAIVALGKELGKPVCATGD
                     VHFLDPEDEIYRHILLASKGFEDADEPLPIYFKTTAEMLEEFSYLGKETAYDVVVRNT
                     NLIADWCEPIEPLPKGLFAPKLEDSDGELKRLVWGKAHELYGEEPPQIVVDRINVELG
                     DIIRCKYDVI"
BASE COUNT          932 a         1503 c         1692 g          899 t
ORIGIN      
        1 gcttggcgcg ccagatatgc gtctgcccat ccaatatgcc ctgacatggc ctaagcggac
       61 gcccggtccg gctgtcccac tggacctgtg gaactgtggg ccgctcacct tcggtacgcc
      121 ggatctggat acattccgct gccttcccct ggcactggag gcagcgcgga caggcggcac
      181 agcgggggcc atcttaaacg gggccaatga ggcggcggtg gcccggttcc tggaggggaa
      241 gatcgggttc ctggacatcg cagaacaggt cgcccgggcc atggatcagg tccctgtggt
      301 ccagcggccc actctggagg acattctgga ggcggaccgg gcggcccgcg ccgtatgaga
      361 caaggtccag actgattttc ttttggaggt tctatgctcg gttctgttct tttttatgtt
      421 gtcatcgcgg tactgatctt cggcgtgctg atcgccaccc atgagctggg gcattttgcc
      481 accgccaagc tgctgggggt gcaggtgaat gagttcgcgg tgggcatggg gcccgctctc
      541 tggaagaagg agaaggggga gacactttac agtattcgtg ccctgcccat cggcggatac
      601 tgcgccatgg agggggagga tgaggagagc gacaaccccc gggccttcgg ccgggccagc
      661 gggtggaaaa agatcgtcat cctctgcgcc ggggctttca tgaattttct gaccggcttt
      721 gtgctgatcc tgatcctttt ttcccaggcc accggtttcg tgctgcccac catcgacggc
      781 tttctggagg gctacggtgt ggaggacagc gggctccagc ccggggacgt ggtctactcc
      841 ctggacggac accggatcta caattataat aacctgacct tctttctcgg ccgggcgggg
      901 gataccctgg atttcgtggt gctccgggag ggcgagaagg tccgcctgga cggcgttgag
      961 atgcccctcc aggcccgcac ggatgaggag gggaagacca catacctgcg ggggatcagc
     1021 gtggggcggg agatcatccc cgccaccctg ggcagcaagc tgctgtacag ctggtacaac
     1081 agcctggatt ttgtgcgcac ggtctggatc agcctgggag atctggtctc cggggtggtt
     1141 ggactgcggg acctgtcagg cccggtgggc atcgtgacca ccatctccca ggtggggacc
     1201 cagtctgaga cggtggggga cgcggtgtat aacatcctct acctgtctgc gctgatcgca
     1261 gtgaacctgg cggtgatgaa tctgctgccc ctccccgcgc tggatggggg acgcattttc
     1321 tttctgttcc tgaacggcct gctcttcggg ctgttccgca gaaagatcga cccaaagtat
     1381 gaggggtacg tccatatggc ggggctggcc ctgctgctga tcctgtccct ggctgtgact
     1441 ttcagcgacg tgggcaagct gttcggcgtc tagccggacc agatatggga aaggaagcgg
     1501 tttcaatgac cagacagatc catgtgggcg gcatccccat cggcggcggg gcgccggtca
     1561 ccatccagtc catgaccaat acccgcaccg acgatgtgga gtccaccctg tcccagatcc
     1621 ggtccctggc tgcggctgga tgtcagatcg tgcgggtggc ggtccctgat ggggccgccg
     1681 cccgggcggt ggccaagatc aaggaggcca gtccgctccc agtggtggtg gatatccact
     1741 tcgactataa gctggccctg gaggccatcg cggccggagc ggacaaggtc cgcatcaatc
     1801 cgggcaatat cggtggagag gaccgggtca aggcggtggc agacgcctgc cgtctgcggg
     1861 gcatcccaat ccgcatcggt gtcaatggcg gctctctgga gaagggactg ctggccaaat
     1921 acggcggcgt ctgtccggag gccatggtgg agtcagcctt cggccacatc cgtctgctga
     1981 acaaattcga ttttgacgac atctgtgtgt cgctgaagtc ctccagcgtc cccatgacca
     2041 tgcgggccta tcagctgatg agagagaaaa gcaattatcc tttacatatt ggcgttaccg
     2101 aggccgggac ggtccggatg ggcaccctga agtctgcggt gggcatcggg ggcctgctgg
     2161 ccctggggat cggggacacc atgcgggtct ccctctccgc cgacccggtg gaggaggtct
     2221 acgcggcccg ggacatcctg aaggccgccg gggtccggcg tgagggggca gagctggtct
     2281 cctgtcccac ctgcgggcgg acccgcatcg atctgatcgg cctggctgcc caggtggagg
     2341 agcggctgaa gggggtggac aagcccatca cggtggcggt gatgggctgt gccgtcaacg
     2401 ggccggggga ggcgtctgcc gccgactgcg ggatcgcggg cggcatcggc gagggcctcc
     2461 tgttccgaaa aggggagatc gtgaaaaagg tcccccagga ccggctggtg gacgagctgt
     2521 ttgccctgat cgaggaactg taacacaggg ggcgctccac catcccactg actgagaatc
     2581 aagcgcaagg agcatctgca tcatgtccaa gaagatccca tttttacaaa tgtttgctgc
     2641 cctgtgccag tggcaggagt tggcggaggc cgtagagggc tgggtcattg tggccgcggc
     2701 cattgacaag gccagccgga gcgccctggt gaccctggat ggggccaggg gagccggggc
     2761 caatctgatc cgccaggcag aggaggccgt ggcccggtgc tacgggctcg cctcagtgaa
     2821 gtttcagatg gcacccccgg agccggagac ccccgccgaa gcgccggcac cggcggctcc
     2881 ttctgtgcct gcctccgcgc cccagacccg cccggcccat cctcggcagg gggagagcac
     2941 ggcaccggag atggacgcct tcgcgcggac ggaggccatt cggcgggagg cgctgcggaa
     3001 ggcccggggg agcgcgccgg cggccagacc caagggaaaa ggggagggca agccctccgg
     3061 caagacgatc ttcgggcatg tcatcaagaa gcggcccacc cccatcgggg agctggagct
     3121 ggacatgggc atggtggtca tcgagggcga tgtctttgcc gtggacaacc gggagctgaa
     3181 aaagcggggc gcctgggtgg tggccttcga tctgaccgac tacaccggct ccattcgggt
     3241 gaacaaattc ttccccggag acgagggcaa gcccatcgtg gacggggtca agaaggggat
     3301 gcacctccag atccagggcc ggctgaacat ggaccggttc tacggcgaca tggtgctgga
     3361 gcccatgtcc atcatggcgg tgcccaagcc tatgaagatg gatgacgcgc cggaaaagcg
     3421 ggtggagctc cacctgcaca ccaccatgtc cgctatggac gccctcacct cggtcggtcc
     3481 caagctgggg ccggacaaaa acgtggtcaa gcgggcggag gcctggggcc accgggccat
     3541 cgccatcacc gatcacgggg tggcccagtc cttcccggat gcgtggcact ccgccaaaaa
     3601 tatcaagatc ctgtacggcg tggaggcgta ctacatcaac gacgtggatg acagagtagt
     3661 ggtccacggg gaaacagaac agccctttga ccaggagatc gtctgcttcg acatcgagac
     3721 cacagggctg aaccggaagt acgaggtcat catcgagatc ggcgccgtag tgctgaaaaa
     3781 cggggagatc accgaccgct tcaacacctt tgtctccccc ggccgcatcc tgagtccgga
     3841 gatcatccgc ctgaccggta tcaccgatga gatgctggtg ggggccccct cccaggagga
     3901 ggccctgcgg gctttcctgg cttttgccgg ggaccggccc ctggcggccc acaacgccga
     3961 cttcgacatg ggctttatcg ccgccggctg ccggaaatac ggcattccct tccacaatcc
     4021 ctccatcgac agcctgatcc tggcccagaa cctgctgcct gacctgggca agtacaagct
     4081 ggacatcgtg gcggagcacc tgcacctgcc cgccttcaat caccaccggg ccagcgacga
     4141 cgcggccacg gtgggctaca tgttgccccc gttctttaag atgctggagg agatgggcct
     4201 gcgccacctg ggggagatca acggggccat ggtccacctg cgcaagggag gaaaggccaa
     4261 gcgccagccc aagcacctga tcgtgctggc gcggaaccag accggtctgc gtaacctgta
     4321 caagctcatc tccctgggac atctggacta cttcaagcgt taccccatca tgctcaaaag
     4381 cgtcatcaac gagaaccggg aggggctgat cctgggctcc gcctgcgagg cgggcgagct
     4441 cttccgggcg gtggcggacg gcaaggactg ggaggagctc aagcgcatcg cctcctggta
     4501 cgattatctg gagatccagc ccctgtgcaa caatatgttc atgctccgca aggggatggt
     4561 ccgcacagag gaggagctgc gggacttcaa ccgggccatc gtggccctgg ggaaggagct
     4621 gggcaagccg gtgtgcgcca ccggtgacgt ccacttcctg gacccggagg acgagatcta
     4681 ccgccacatc cttctggcct ccaagggctt tgaggacgcg gatgagcccc tgcccatcta
     4741 cttcaagacc accgccgaga tgctggagga attttcctat ctggggaagg agaccgccta
     4801 cgacgtggtg gtgaggaata ccaatctcat cgcggactgg tgcgagccca ttgagcccct
     4861 gcccaaagga ctgtttgccc ccaagcttga ggactccgac ggggagctga agcggctggt
     4921 atggggcaag gcccatgagc tctacggcga ggagccgccc cagatcgtgg tggaccgcat
     4981 caacgtggag ctgggggata tcatccgctg caagtacgac gtgatc
//
LOCUS       DBMDSJ010000081         4965 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4693_length_4965_cov_1.755757, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000081 DBMDSJ010000000
VERSION     DBMDSJ010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4693_length_4965_cov_1.755757"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /gene="polA"
                     /locus_tag="ACZPAQ_03035"
     CDS             complement(<1..2072)
                     /gene="polA"
                     /locus_tag="ACZPAQ_03035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR0902986.1"
                     /translation="MKKLMVLDGNSIVNRAFYGVSQNLTTRDGQPTNAVFGFLAILNK
                     LLDECEPQALCVTFDRKAPTFRHLAYEGYKATRKGMPDELASQMPILKEVLAALNIPM
                     YELDGWEADDLIGTISVKDTAAGWETVVVTGDKDSLQLVTETTTVKLVSTRMGRTATR
                     DMTPETFQEEYGFGPIHIIDLKALMGDASDNIPGVKGVGEKTAMALVQRYGSIDLLYA
                     AMPEIEMAAGTPAKPGVVKKLAEGEEMARMSYDLATIRTDAPIEFTPEANLRREPDRA
                     ALYQLFLRLEFAKLIDKYGLTAPQGEGDTETAPVTGTCESEVVESRERMEELLALWRG
                     LDQVDVLALPGLDTVCVEWQESESLSRAALFFAGRLDCHNQFLRELFAPGIRKAAHGV
                     KELWKALLDEGIEPGDFIFDTEVAAYLLAPTDGSYELEKLGMTYYNQEFPKAAAYLEE
                     GAFGPLADPVAPMGALISHCALIGALHQTLRKRLEELDLWTLYQQIELPLCPVLAEME
                     REGMLVDRGALIRFGEMLSQGIQGAQAAIFALAGEEFNINSTQQLGRILFDQLGLPPV
                     KKTKTGYSTNADVLEKLRGQHPIIETILEYRQLTKLKSTYVDGLSKVIGADGRIHTCF
                     QNTVTATGRLSSAEPNLQNIPVRTELGAQLRKMFVAGPGKVLVDADYSQIELRLLAHM
                     AEDEHMIGA"
     gene            2270..2803
                     /locus_tag="ACZPAQ_03040"
     CDS             2270..2803
                     /locus_tag="ACZPAQ_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0902987.1"
                     /translation="MERKTSSLRFTRGRLAALLSAVVLVTIVVFSLTFYHLCLHAKRM
                     EVLTQGDGPLWAFEQGQLDILSQEADGATVYGGSGCRVTVRNGEITAVTDENMGFSKF
                     YEGGVLTSVTFDNPLAEAAALCTPFSVNSYVQYTVRTPEEARTIRAELDRGGTAALEA
                     RGLRCWALGNGYLVVET"
     gene            complement(2862..3452)
                     /locus_tag="ACZPAQ_03045"
     CDS             complement(2862..3452)
                     /locus_tag="ACZPAQ_03045"
                     /inference="COORDINATES: protein motif:HMM:NF014573.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR0902988.1"
                     /translation="MKQVLWINSCMRGAGQSRTLELCRTFLEELRVRRPGLKVAERDL
                     TRAELPVMTAELSRRREELAAEGREDPLLLPAREMARADLVVVGAPYWDLGFPAALKV
                     YLEWACTLGITFGYTQAGEQRGLCRAERLIYITTAGGPLEDRNFGYEYVRGVAGMLGI
                     INTHCVAAQGLDIRGNDPEAILREAQERAARLAAEI"
     gene            3717..4451
                     /locus_tag="ACZPAQ_03050"
     CDS             3717..4451
                     /locus_tag="ACZPAQ_03050"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR0902989.1"
                     /translation="MKTDILGIRFDDLTREEAAQAGAGLLRSGSFHYVVTPNPEFILS
                     SEKDPEFRRILNEADLVLPDGIGVVYSAKILGTPLKERVPGIDFAADMLAQLNEMGGR
                     LFLLGAKPGVAEEAGSRICTQYPNLVLCGTHDGYFDNEAPVVRAVSQAAPDLLFVCLG
                     APKQEKWMARWGALTGAKLAIGLGGALDVFAGNVDRAPEKWRRMGLEWAYRLSREPKR
                     IGRMAKLPLVLTRSLGARLRGKGGRS"
     gene            4451..>4965
                     /locus_tag="ACZPAQ_03055"
     CDS             4451..>4965
                     /locus_tag="ACZPAQ_03055"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR0902990.1"
                     /translation="MPGKLIVFEGTDGSGKSTQFQALCARVEQTGTPFRRLVFPQYQE
                     PSSALIRMYLGGEFGSRPGDVNPYAASAFYAVDRYASLKKVWGEYYSQGGLILTDRYT
                     TSNAVHQGAKCAPQERPAFLGWLDDFEHDKLGLPRADLVLYLDMPTRCSLELLRSREA
                     ATHTQGDIHEV"
BASE COUNT          812 a         1670 c         1492 g          991 t
ORIGIN      
        1 gcgccgatca tgtgctcgtc ctccgccatg tgagccagca ggcgcagctc gatctgggag
       61 tagtccgcgt ccaccagcac cttgcccggt cccgccacga acatcttccg cagctgcgcc
      121 cccagctcgg tgcggacggg gatattctgg aggttgggct ccgccgagct gagccgcccg
      181 gtggcggtca ctgtgttctg gaagcaggtg tggatccgcc catccgcccc gatcaccttg
      241 gagagcccgt ccacataggt ggatttcagc ttggtcagct gccggtactc caggatggtc
      301 tcgatgatag ggtgctggcc ccgcagcttc tccagcacgt cggcgttggt ggagtagcca
      361 gtcttggtct ttttcaccgg gggcaggccc agctggtcaa agaggatgcg gcccagctgc
      421 tgggtggagt tgatgttgaa ctcctccccc gccagcgcaa agatggctgc ctgggccccc
      481 tgtatcccct gggagagcat ctctccgaag cggatcagcg ccccccggtc caccagcatc
      541 ccttcccgct ccatctccgc cagcactggg cagaggggca gctcgatctg ctggtacagc
      601 gtccacaggt ccagctcctc cagccgcttt ctcagcgttt ggtggagggc cccgataagg
      661 gcgcagtggg atatcagggc gcccattggg gccaccgggt cggccagggg gccgaaggcc
      721 ccctcctcca gataggcggc ggccttgggg aactcctggt tgtagtaggt catccccagc
      781 ttctccagct cgtagctgcc gtcagtaggg gccaggagat aggccgccac ttcggtgtca
      841 aagatgaagt ccccgggctc gatcccctcg tccagcaggg ctttccaaag ctccttgacc
      901 ccatgggccg ccttgcggat gcctggggcg aagagctccc tcaagaactg gttgtggcag
      961 tccagccggc cggcgaaaaa cagcgccgcc cgggagaggc tctcactctc ctgccactcc
     1021 acgcacacgg tgtccagccc gggcagagcc agcacatcca cctggtccag cccccgccac
     1081 agggcaagca gctcctccat cctctcccgg ctctccacca cctcgctctc acaggttccg
     1141 gtgaccgggg ccgtctctgt atccccctct ccctggggtg cggtgagtcc gtatttatcg
     1201 atgagcttgg caaactccag ccggaggaag agctggtaca gggcggcccg gtccggctcc
     1261 cggcgcaggt ttgcctccgg tgtgaactcg atgggagcgt cggtgcggat ggtggccaga
     1321 tcataggaca tgcgggccat ctcctccccc tcggccagct ttttgaccac tcccggcttg
     1381 gctggggtgc cggcggccat ctcgatctcc ggcatggccg cgtacagcag gtcgatggat
     1441 ccataccgct ggaccagggc catggccgtc ttctccccca cccctttcac cccggggatg
     1501 ttgtcgctgg catcccccat gagggctttc agatcgataa tgtggatggg cccgaagccg
     1561 tactcctcct gaaaggtctc cggggtcata tcccgggtgg cggtacggcc catgcgggtg
     1621 gacaccagct tgacggtggt ggtctctgtc accaactgga gggagtcctt gtcccctgtg
     1681 accaccacgg tctcccaccc ggcagcagta tccttcaccg agatggtgcc gatgaggtcg
     1741 tccgcctccc atccgtccag ctcgtacatg gggatgttca gggccgcgag gacctccttc
     1801 aggatgggca tctgactggc cagctcgtcg ggcatcccct tgcgggtggc cttatagccc
     1861 tcataggcca gatgacggaa ggtgggggcc ttccggtcaa aggtgacaca cagtgcctga
     1921 ggttcgcact cgtccagcag cttgttcagg atggccagaa agccgaacac cgcattggtg
     1981 ggctggccgt cccgggtggt caggttctgg ctcacgccgt aaaaggcccg attgacaatg
     2041 gaattcccat ccaggaccat cagttttttc atagggtctg tcctcctgtt tccggcccac
     2101 agccggagga atgatcgaat gatttattgt atagtatatc agcttttccc ctggcaaaca
     2161 agataaaagc ccccctggct ccttgcagtt tttccccttg tatggtattc tgaggacaga
     2221 gttccccccg aatatgtcat agcttttatt gtcaggaggc tgatgagtaa tggagcgaaa
     2281 gacttcttcc ctccggttca cccgtgggcg actggccgcc ctgctgtcgg ccgtggtgct
     2341 ggtgaccatc gtggtgttct ctctgacctt ttatcacctg tgcctgcacg ccaagcggat
     2401 ggaggttctc actcaggggg acggaccgct atgggccttt gagcagggcc agcttgatat
     2461 cctgtctcag gaggcggacg gcgccacggt ctacggcggc tccggatgcc gcgtcacagt
     2521 ccggaacgga gagatcaccg cagtcacgga tgagaacatg gggttctcca agttctacga
     2581 ggggggcgtt ttgacctccg tcacctttga caatcccttg gcggaggcag ctgctctgtg
     2641 cacccccttc tctgtaaact cctatgtgca gtacacggtc cgcaccccgg aggaggcccg
     2701 gaccatccgg gcagagctgg accggggcgg gaccgccgct ctggaggcgc gcggcctgcg
     2761 ctgctgggcg ctgggcaacg gctatctcgt tgtggagaca tgaactcccg ccgcatcaca
     2821 gaagaaaaca cagccgccag cgtactggcg gctgtgtttt cttaaatctc tgcggccagc
     2881 cgggcggccc gctcctgggc ctcccgcaaa atggcctccg ggtcattgcc ccggatatcc
     2941 agtccctggg cagcgacgca gtgggtattg atgatcccca gcatcccggc cacccctctg
     3001 acatactcat atccgaaatt ccgatcctcc agcggcccgc cggcggtggt aatataaatc
     3061 agccgctctg cccggcacag cccccgctgc tcccccgcct gtgtgtatcc aaatgtgatc
     3121 cccagtgtgc aggcccactc cagatagacc ttcagcgccg ccgggaatcc cagatcccaa
     3181 tagggggctc ccaccaccac cagatccgcc cgggccatct cccgggcggg gaggagcagc
     3241 gggtcctccc gcccctccgc ggccagctcc tccctgcgcc gggacagctc cgccgtcatc
     3301 acgggcagct ccgccctggt caggtcccgc tccgccacct tcagccccgg gcggcggacc
     3361 ctcagttcct ccaaaaatgt gcggcacagc tccagcgtcc gggactgtcc cgcccccctc
     3421 atacagctgt tgatccacag tacctgtttc atgcctgtcc tccttttcct cgggcctctg
     3481 cccattttct ctccattatg ccccttctcc gtcccggcgt caaccacgct ttgcctttcc
     3541 cgtgcttttt catagacttt tccgttttcc catgatataa tacccggaga atcgcggaga
     3601 tccccgatat ttttccggtc ctggcctgcg ggcggggctg tttatgttgc atgatcccgc
     3661 ccccgggccc gctgcccggc cgggctgtat tctgttcaag ttctggagga agcactttga
     3721 aaacagatat tttgggcatc cgtttcgatg acctcacccg ggaggaggcc gcccaggcgg
     3781 gcgccggcct gctccgctct ggcagctttc actatgtggt gacccccaac cctgaattta
     3841 tcctgtcctc agagaaggac ccagagttcc gccgcatcct gaacgaggcc gatctggtcc
     3901 tgccagacgg catcggcgtg gtttattccg ccaaaatttt gggcacacct ttgaaggagc
     3961 gggtacccgg catcgacttt gccgctgaca tgctggccca gctcaacgag atggggggcc
     4021 gcctcttcct gctgggtgcc aagcccggcg tggcggagga ggcaggcagc cgcatctgta
     4081 cccagtaccc caatctggtc ctctgcggca cccatgacgg ctactttgat aacgaggccc
     4141 ccgtggtccg ggcggtgtct caggccgctc ccgacctgct gttcgtctgc ctgggcgcgc
     4201 ccaagcagga gaagtggatg gcccggtggg gcgcgctcac cggcgccaag ctggccatcg
     4261 gcctgggcgg cgccctggac gtctttgccg gcaacgtgga ccgggcaccg gagaagtggc
     4321 gcagaatggg gctggagtgg gcctaccgcc tgagccggga gcccaagcgc atcggccgga
     4381 tggccaagct gcctctggta ctgaccagat ctctgggggc ccgcctccgg ggcaaggggg
     4441 gccggagctg atgccgggaa agctgatcgt ctttgagggg acggacggct ctggcaagtc
     4501 cacccagttt caggcgctgt gcgcccgggt ggaacagacg ggcaccccct tccgccgcct
     4561 ggtcttcccc cagtaccaag agccctcatc cgccctcatc cgcatgtatc tgggcgggga
     4621 attcggctcc cgcccagggg atgtgaatcc ctacgccgcc tccgctttct atgcggtgga
     4681 ccggtacgcc tctttgaaaa aagtatgggg ggaatactat tcccaggggg ggctgatcct
     4741 caccgaccgc tacactacct ccaatgccgt ccaccagggg gccaagtgcg ccccccagga
     4801 gcgccccgcc tttctgggct ggctggacga ctttgagcac gacaagctgg gcctgccccg
     4861 ggccgacctg gtcctctatc tggatatgcc tacccgctgc tccctggagc tgctccgcag
     4921 ccgggaggca gccacccaca ctcaggggga catccacgag gtgga
//
LOCUS       DBMDSJ010000082         4950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4700_length_4950_cov_1.713549, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000082 DBMDSJ010000000
VERSION     DBMDSJ010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4700_length_4950_cov_1.713549"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="ACZPAQ_03060"
     CDS             <1..753
                     /locus_tag="ACZPAQ_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013740.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR0902991.1"
                     /translation="VVGTVEDTVNALIQRLQAVDPTALLSAAKAATDWAMRVPEFLLA
                     LVVFVMASYFLTADYPYHRTRAVQHLDESLLRFLQQVKATALAAFGGYLRAELLLSVG
                     VFFILLGGFLLVGQNYALLLALGLAILDFIPIIGSGTVMVPWAVVALFTRDFSTAVYL
                     MVIWGVVAMFRRVAEPRFVGDQTGLSPILSLVGIYVGMKVAGVAGMILGPILILVVLN
                     LVGLGIFQGFWMDVTAAARDITAILSQRPERK"
     gene            908..2284
                     /locus_tag="ACZPAQ_03065"
     CDS             908..2284
                     /locus_tag="ACZPAQ_03065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR0902992.1"
                     /translation="MANQDNEFNFYNSENDGYRGGFGGYTDSNPNQSQGGPEAPQGPQ
                     KHRGSGRRIAALILACAVVGGGAGVGGAALYSSVFASQPSVIYQNTQPISTQADPVAS
                     SSGQAMTPEQLYAANVDACVGITVSTTMTNIFGYTSTSAASGSGFVITADGYIVTNYH
                     VIEDAVKDSSVSITVSFHDGSSYPATLVGGEEDNDVAVLKIEASGLQTVTLGDSDQLK
                     VGQAVMAIGNPLGELTYSLTDGLISALDRLITTGSGNDSTTMNMLQTNCAINPGNSGG
                     PLFNSYGEVIGITTAKYSTSSSGTNVEGLGFAIPINDVKNIISDLIQYGYVTGKPYMG
                     ITGVTMSASVAQQYGVTNAVLGVYVATVEDGSCSAKAGLQPGDIITAMDEDDVVTFQD
                     LEAAKATHQAGDTVTLTVFRNGEKLELSLTLDEQPRETITDTSSQQQQQPQQNNQYYG
                     WPFGGFFS"
     gene            complement(2417..3478)
                     /locus_tag="ACZPAQ_03070"
     CDS             complement(2417..3478)
                     /locus_tag="ACZPAQ_03070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0902993.1"
                     /translation="MSEEQSFLEQLPQAAVEVRDGKITALNSAALSHLPELAVGAQPP
                     QYLSAPLDGVQTAGAFTGGGGLFVFSKVSSPGGVLLLFRPAPDSALSPQQMDGFARQL
                     REQMGGLLNNLQLLSQDLSGTGSPTDRRLASINRNFYQMLRLVNNLEFLRDTKSQSGV
                     AFSPVTMDLAGLCRHTAEDTAPLLAQAGIQLRYQPSTEGLLIPGDAALLSRMLLGLLS
                     NAAKAAPSGTVLLTLRRWGDRAVLTCTNSGSTPDDGQLSALLEGASPHLLPEPGEGAG
                     MGLPIIRHIVALHGGTLLLERREPGGLAATVSLPTGPLSMDLTLSTPAPERSGGLSPL
                     LVELSDVLPSSLYQPEDIE"
     gene            3829..4839
                     /gene="gap"
                     /locus_tag="ACZPAQ_03075"
     CDS             3829..4839
                     /gene="gap"
                     /locus_tag="ACZPAQ_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR0902994.1"
                     /translation="MSIKVGINGFGRIGRMVFRASLDHPEIEIVGINDLCPAEYLAYM
                     LKYDTMHGICKAEIGSTENAIVVNGKEIPVSAERNPADLPWAKLGAEYVVESTGLFLT
                     KEKAQGHLDAGAKKVIMSAPSKDDTPMFVCGVNLDKYTTDMNFVSNASCTTNCLAPIA
                     KVLNDNWGITEGLMTTVHSVTATQKTVDGPSLKDWRGGRAATGNIIPSSTGAAKAVGK
                     VIPELNGKLTGMSMRVPTLDVSVVDLTVNLAKPAKYEEICAAMKAASEGELKGVLGYT
                     DEAVVSSDFLGDTRTSIFDADAGIALTDTFVKVVSWYDNEIGYSNKVLELIEHMYSVD
                     HK"
BASE COUNT          969 a         1526 c         1580 g          875 t
ORIGIN      
        1 gtggtgggga cggtggagga caccgtcaac gccctcatcc agcgcctcca ggcggtggac
       61 cccaccgccc tgctgagtgc ggccaaggcg gccaccgact gggccatgcg ggtgccggag
      121 ttcctgctgg ccctggtggt ctttgtgatg gcctcctatt tcctcaccgc ggactacccc
      181 tatcaccgca cgcgggcggt ccagcacctg gacgagagcc tgctccgctt cctccagcag
      241 gtgaaggcca ccgccctggc cgccttcggc ggctacctgc gggcggagct gctgctgtcg
      301 gtgggggtgt tcttcattct gctgggggga ttcctgctgg tggggcagaa ctacgccctg
      361 ctgctggccc tggggctggc cattctggac tttatcccca tcatcgggtc gggcaccgtt
      421 atggtgccct gggcggtggt ggccctgttt acccgggatt tctccaccgc tgtctatctg
      481 atggtgatct ggggggtggt ggccatgttc cgccgggtgg cggagccacg gttcgtgggc
      541 gaccagaccg gcctgtcccc tatcctgtcc ttggtgggca tctacgtggg catgaaggtg
      601 gccggagtgg ctggcatgat cctgggcccc atcctcatcc tggtggtgct gaacctggta
      661 gggctgggga tctttcaggg cttttggatg gatgtgacgg cggcggcccg ggacatcacg
      721 gcgatccttt cccagcggcc ggagcggaaa tgagagggtc tgtcaaagtt ttttcgggaa
      781 ttgttcaaaa aatcccccct ctttcttcat gctttcttca tatttagacc gtatccttat
      841 ttttgtcaga aggggaaaga gaggcgctcc ccccggacac agctcaaatc ggcaaggagt
      901 gtttgaaatg gcaaatcagg acaatgagtt caatttctat aatagtgaga atgacgggta
      961 ccggggcggc ttcggcgggt acaccgattc caaccccaac cagagccagg gcgggccgga
     1021 ggccccccag gggccccaga agcacagggg atccggcagg cgcattgcgg cgctgatcct
     1081 ggcctgcgcg gtagtgggcg gcggagcggg cgtgggcggc gcagccctgt actccagcgt
     1141 cttcgcgtcc cagcccagcg tgatctatca gaacacccag cccatctcca cccaggcgga
     1201 ccctgtggcc agctccagcg gccaggccat gaccccggag cagctgtacg ccgccaatgt
     1261 ggacgcctgc gtgggcatca cggtgtccac caccatgacc aacatcttcg gctataccag
     1321 cacgtcggcg gcctccggtt ccggcttcgt catcaccgcc gacgggtaca tcgtcaccaa
     1381 ctaccacgtc attgaggacg cggtgaagga cagctcggtg agcatcaccg tctccttcca
     1441 cgacggcagc agctaccccg ctaccctggt ggggggcgag gaggacaacg atgtggccgt
     1501 gctgaagatc gaggcctccg ggctccagac cgtgaccctg ggcgactccg accagctgaa
     1561 ggtgggccag gccgtgatgg ccatcggcaa ccccctgggc gagctgacat actccctcac
     1621 cgacggcctc atctccgctc tggaccggct cattaccacc ggctccggca atgactccac
     1681 cacgatgaac atgcttcaga ccaactgcgc catcaacccg ggcaactccg gcggtcccct
     1741 gttcaacagc tacggggagg tcatcggcat caccaccgcc aaatacagca cctccagctc
     1801 cggcaccaat gtggagggcc tgggcttcgc catccccatc aacgatgtga agaacatcat
     1861 ctccgacctg atccagtacg gctatgttac cggaaagccc tacatgggca ttaccggcgt
     1921 gaccatgtcg gcctctgtag cgcagcagta cggcgtcacc aacgcggtgc tgggtgtcta
     1981 tgtggccaca gtggaggacg gctcctgctc cgccaaggcg ggactgcagc ctggggacat
     2041 catcaccgcc atggatgagg acgacgtggt cactttccag gacttggagg ctgccaaggc
     2101 cacccatcag gcgggggata ccgtgacgct gacggtgttc cgtaacggag agaagctgga
     2161 gctgtccctt accctggacg agcagccccg ggagacaatc accgatactt cctcccagca
     2221 gcaacagcag cctcagcaga acaaccagta ttacggctgg cccttcgggg gatttttctc
     2281 ctgagcgggg agcggcccca tactgagaac agaatggtcc gggtccggca gccaacggct
     2341 gccgggcccg tttgctgcat caaaaaaacc accggcctgg ccggtggttt tctgatcttg
     2401 tgagaggcgg gcagcgctat tctatgtcct ccggctggta cagagaggag gggaggacgt
     2461 cggacagctc caccagcagg ggagataggc cgccgctccg ctccggggca ggggtgctca
     2521 gggtcagatc catggagagg gggccggtgg gcagagagac ggtggcggcc aggccgcctg
     2581 gctcccggcg ctccaggagc agcgtgccgc cgtggagagc gacgatgtgc cggatgatgg
     2641 gcaggcccat tcccgccccc tctccgggct ccgggagcag gtggggggag gccccctcca
     2701 acagagcgga gagctgtccg tcgtcaggag tggagccgct gttggtacag gtcaggacgg
     2761 cccggtctcc ccaacggcgc agggtgagca ggacggtccc gctgggggcc gccttagccg
     2821 cgttggacag caggcccagc agcatacggg agagcagggc cgcgtcgccg gggatcagaa
     2881 gtccttcggt gctcggctga tagcgcagct ggatgccggc ctgagcgagc aggggggcag
     2941 tgtcctccgc tgtgtggcgg cacaggccgg ccagatccat ggtcaccggg gaaaaggcca
     3001 ccccggactg ggacttggta tcccgcagga actccaggtt gttgaccagg cggagcattt
     3061 gataaaagtt gcggttgatg gaggccagcc gccggtcggt gggggagccg gtgccgctca
     3121 ggtcctggga gaggagctga aggttgttca gcagcccccc catctgctcg cggagctggc
     3181 gggcaaagcc gtccatctgc tggggggaga gggcggagtc aggggcgggg cggaacagga
     3241 gcagcacccc tccgggggaa gagaccttgg aaaagacgaa caggccgccg ccgccggtga
     3301 aagctcccgc agtctgaacg ccgtccagcg gggcagacag gtactggggc ggctgggccc
     3361 ccacggccag ctctggcagg tgactgaggg cggcggagtt gagcgcggtg atctttccgt
     3421 cccgcacctc caccgccgcc tgggggagct gctccaaaaa gctctgttct tcagacatcc
     3481 agatcctcct ttccgctcca aaaccggcgc agcacttccg caataggatt tgcggggcgg
     3541 ctgattttag aaatggaaaa tttcgctggg ccggggcgtg agcccggccc ggacaggacc
     3601 tagtatatca gaaatttgct gcatcaacaa gaacatgggc gcagattctg gcgaactgtg
     3661 taaatccatg ggaaaaaaga acagaaggca gggcctccta tgccctgagg tggctaattt
     3721 ccacggagaa acagagaaaa aactatgcaa aacacagact accaaaatca gaaaaaaagg
     3781 gatataatag tggagcgcaa ccaaaacttg ataaaggaga cagttacaat gagcattaaa
     3841 gttggcatca atggctttgg ccgcattggc cgcatggtct ttcgggccag cctggaccac
     3901 cccgagatcg agatcgtggg catcaacgac ctgtgccctg ccgagtatct ggcctatatg
     3961 ctgaagtatg acaccatgca cggcatctgc aaggctgaga tcggcagcac tgagaacgcc
     4021 atcgtggtca acggcaagga gatccccgtc tccgccgagc ggaaccccgc cgacctgccc
     4081 tgggccaagc tgggcgcgga gtatgtggtg gagtccaccg gtctgttcct gacgaaggag
     4141 aaggcccagg gacacctgga tgccggtgcc aagaaggtca tcatgtccgc cccctccaag
     4201 gatgacacgc ccatgttcgt gtgcggcgtc aacctggaca agtacaccac tgacatgaac
     4261 ttcgtgtcca acgcctcctg taccaccaac tgcctggccc ccatcgccaa ggtgctgaat
     4321 gacaactggg gcatcactga gggcctgatg accaccgtgc actccgtcac tgccacccag
     4381 aagactgtgg acggcccctc tttgaaggac tggcgcggcg gccgtgctgc caccggaaac
     4441 atcattccct cctccaccgg cgctgccaag gccgtgggca aggtcatccc cgagctcaac
     4501 ggcaagctga ccggcatgtc catgcgcgtc cccactctgg acgtgtccgt ggtggacctg
     4561 actgtcaacc tggccaagcc cgccaagtat gaggagatct gtgccgccat gaaggccgcc
     4621 tctgagggtg agctgaaggg tgtgctgggc tacaccgacg aggccgtggt gtccagcgac
     4681 ttcctgggtg acacccgtac ctccatcttc gacgccgatg caggcattgc cctgaccgac
     4741 actttcgtaa aggtcgtctc ctggtacgac aacgagatcg gctactccaa caaggtcctg
     4801 gagctgatcg agcacatgta cagcgtggac cacaagtaag cactgtcctg tttcagggtg
     4861 ccgccatcta gcggcgccct gttttttaaa aagagcaggc gcctcacgga tgcgccgccc
     4921 cagacagaac agaaaggcgt ccgctcccca
//
LOCUS       DBMDSJ010000083         4927 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4719_length_4927_cov_1.697058, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000083 DBMDSJ010000000
VERSION     DBMDSJ010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4719_length_4927_cov_1.697058"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="ACZPAQ_03080"
     CDS             complement(<1..888)
                     /locus_tag="ACZPAQ_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR0902995.1"
                     /translation="MKNEKLRNIAIIAHVDHGKTTLVDEMLKQSGVYRENQAVVERVM
                     DSNDLERERGITILAKNTAIQYQDVKINVVDTPGHADFGGEVERILKMVNGVILLVDA
                     AEGPMPQTRFVLSKALELGHRVIVVVNKIDRPDQRVYEVIDEVLELLLDLDATPEQLD
                     SPMLFCSGRQGTASVQPDVAGSDLKPLFDTILEYIPAPDADVDAPFQMLVSSIDYNEF
                     VGRIAIGRIERGAIKQNQEIAVCNYHDPDAAPKKAKATALYEFDGLGKVPVTESTAGN
                     IIAMSGIGDVTIGDTICAPD"
     gene            1632..3152
                     /locus_tag="ACZPAQ_03085"
     CDS             1632..3152
                     /locus_tag="ACZPAQ_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR0902996.1"
                     /translation="MDEVLHKPAGRRPRQSDGRELSAQEQLGLLARRVVTDWKHFYQW
                     ARFHGRRVIHTVHDLAGGNRATGPVTFLAVSGALGLALTVSTLYAPAYTVTVDEDTLG
                     VVSDQAVVADAVAQVERRGTEILGHDYQVEGQIGYDFTLSLRSELTQPDQVENYFLEQ
                     LSQVADGLRRYQVSVNGVAVGSVEEMGALESVLDQLKGQYVNENTISAEFVDDIQVED
                     VYSEEGQMTAEELQTALMANTTGDTTYTVVKGDTFNAIAYANDMSVSDLKALNPGIDI
                     NRLMIGDVLNVKEQIPLLSVTTVDDVTYTEPIACPVEEVKDSSMYVGDSKILTQGTEG
                     EAQVHAQVTYVNGSETQREILSSTTLREPTTTVKAVGTKEKPKTASTGSYSWPIRGRI
                     TSYFGGRNIFGSYSYHSGIDISASYGAAIKAADGGTVTFAGYKGSYGYLVIITHDNGT
                     QTYYGHNSSLLVSAGQKVYKGQQIAKAGSTGRSTGTHCHFEVRVNGTSVNPLSYLS"
     gene            complement(3316..>4927)
                     /locus_tag="ACZPAQ_03090"
     CDS             complement(3316..>4927)
                     /locus_tag="ACZPAQ_03090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR0902997.1"
                     /translation="PHCWRCKKPIIFRATPQWFCSVDSFKDEAVAACDDVRWLPAWGK
                     DRMISMIRERADWCISRQRRWGLPIPVFYCADCGKPIVTDETIEAVSRLFGEKGSNAW
                     YEMEAKDILPEDFACPHCGSRTGFTKEEDTLDGWFDSGSTHFASMQKDQNFWPADMYM
                     EGLDQYRGWFQSSLLTAVGALGKGAPFQECVTHGWTVDGEGKAMHKSLGNGVDPAEIF
                     KKYGADLLRLWAGSADYHVDVRCSDNIFKQLSQNYLKFRNTARYCLGNLDGFDPDHLV
                     SPEEMLELDRWAVTKLNELIRKCFAAYDNYEFHVVSHAINDFCVVELSSFYLDIIKDR
                     LYCEGVDSLERRSAQTALWMILDTITKLFAPILAFTCDEIWQAMPHREGDDPRNVVLN
                     EMNQPFQDYDMGALVSWTALIQLRDGVNAALEAARNEKKIGKSLEAHITLVRAQDQAD
                     NLTALQEKFQDQWADLFIVSDVEVSDDPALYAQGSDTPIAGVRVLVSEANGDKCERCW
                     KHHPLVGANAAHPTLCPRCAKVVQAIHL"
BASE COUNT          994 a         1426 c         1573 g          934 t
ORIGIN      
        1 gtcgggggcg cagatggtgt ccccgatggt cacgtcgccg atgccgctca tggcgatgat
       61 gttgccggcg gtggattcgg tgacggggac cttgcccagg ccgtcgaact catacagcgc
      121 ggtggccttg gccttcttgg gggcggcgtc ggggtcgtgg tagttgcaca ccgcgatctc
      181 ctggttctgt ttgatggcgc cccgctcgat gcggccgatg gcgatgcggc ccacgaactc
      241 gttgtagtcg atggaggaga ccagcatctg gaaaggggcg tccacgtccg cgtcgggggc
      301 ggggatatac tccagaatgg tgtcgaacag gggcttcagg tcgctgcccg ccacatcggg
      361 ctggacagag gccgtgccct gacggccaga gcagaacagc atgggggagt ccagctgctc
      421 cggggtggca tccaggtcca gcagcagctc cagcacctcg tcgatgacct cgtacacccg
      481 ctggtcgggg cggtcgatct tatttactac cacgatgacc cggtggccca gctccagggc
      541 cttggacagc acgaagcggg tctgaggcat ggggccctcc gcggcgtcca ccagcaggat
      601 gacgccgttg accatcttca ggatgcgctc cacctcgccg ccgaagtcgg cgtgtcccgg
      661 agtatccacg acgttgatct tcacatcctg atactgaatg gcggtgttct tggccaggat
      721 ggtgatgccc cgctcgcgct ccaggtcgtt ggagtccata acgcgctcca ccacggcctg
      781 attttcccgg tacacgccgg actgcttgag catctcatcc accagagtgg tcttgccatg
      841 gtcaacgtgg gcaattatgg caatatttct cagtttttcg ttcttcatca tttttcctcc
      901 taccagcaat atggcaagac cactcggggc cccatcgccg atggggcgcg ccgaatggcg
      961 cgtacaagct tttgtccgga agaaaaagct tgacgcaggc ggaatttatt tccgccaagc
     1021 atttaaatat ctggggtccc tgcggggcat caggcccgcg gggcggccat accgtggccg
     1081 gcgtgggcga tcatggcggc acgtctctgt ttttcgcgct ttgcggccgc ttctcattta
     1141 aagacacaaa accatatgag acgccggaga ggaggctcaa atggcggatc gccgcagatc
     1201 agcgggaagc gctccccaaa ggggaggtgt tcgtgttgac gtaatattat acagcgccct
     1261 ttcgagaaac gcaacatttt ttgcagaaaa acaggaaagg atctatttgg atccccgatt
     1321 tgacacggag aagcggaaaa ataggagagg atattgtgat ttcgccaaaa atttgaggcg
     1381 ggaaaacggg gggcacaggg gcgcccacgg atagagtggg gaggacggga caggggccac
     1441 aagatatcgt gaaaaaaccg gaaaaagacg agaaaaagaa ataaaaaatt tacatttgga
     1501 gaggagaaaa aggggttgac gtaaagcggg attgcgtgta tagtacgttt gtaaccaaat
     1561 tgtaacattt tcttttccgt tctgtcactt ttggggcagc gggaaatttg atctggaaga
     1621 ggtcatgatt catggacgag gttttgcaca agcccgccgg ccgccggccc cggcagagtg
     1681 acggtcggga gctgtcagct caggagcagc tggggctgct ggcgcgccgg gtggtcacag
     1741 attggaagca tttttatcag tgggcccggt tccatggccg ccgtgtgatc cacacggtcc
     1801 atgacctggc gggggggaac cgcgccaccg gtccggtcac ctttctggcg gtgtccggag
     1861 cgctgggact ggcgctgact gtctccaccc tatacgcccc ggcctatact gtcacggtgg
     1921 acgaggatac tctgggtgtg gtgagcgacc aggctgtggt agccgacgcg gtggctcagg
     1981 tggagcgccg aggcacggag atcctgggac acgactacca ggtggagggg cagatcggtt
     2041 atgatttcac cctttccctg cggtctgagc tgacccagcc cgaccaggtg gagaactact
     2101 tcctggagca gctcagccag gtagccgatg gcctgcgccg ctatcaggtc agcgtcaacg
     2161 gcgtggcggt gggctccgtg gaggagatgg gagccctgga gtccgtgctg gaccagctga
     2221 agggccagta tgtgaatgag aacaccatct ccgctgaatt tgtggatgac atccaggtgg
     2281 aggatgtcta ctcggaggag gggcagatga ccgccgagga gctccagacc gccctgatgg
     2341 ccaacaccac tggcgacacc acctacacgg tggtgaaggg agacaccttc aacgccatcg
     2401 cctatgccaa cgacatgtct gtcagcgacc tgaaggcgct gaatccgggc atcgatatca
     2461 accgactgat gatcggggac gtgctcaatg tgaaggagca gatccctctg ctgtcggtga
     2521 ccacggtgga cgacgtgacc tataccgagc ccatcgcatg cccggtggag gaggtgaagg
     2581 actccagcat gtatgtgggg gactcgaaga tcctgaccca gggtacagag ggcgaggccc
     2641 aggtccacgc ccaggtgacc tatgtgaacg ggagcgagac ccagcgggag atcctgtcct
     2701 ccaccaccct gcgtgagccc accacaacag tcaaggcggt ggggaccaag gagaagccta
     2761 agacagcctc cactggaagc tactcctggc ctatccgggg gcggatcacc tcctactttg
     2821 gcgggcggaa tattttcggc agttacagct accactccgg catcgatatc tccgcctcct
     2881 acggtgcggc catcaaggcg gcagacggcg gcaccgtcac cttcgccggc tacaagggga
     2941 gctatggcta tctggtcatc atcacccacg acaacggcac ccagacctat tacggccata
     3001 actcttccct gttggtgtcc gcaggacaga aggtgtacaa gggacagcag atcgccaagg
     3061 cgggcagcac cggccggtcc accggcaccc actgccactt tgaggtgcgg gtgaacggca
     3121 cctcggtgaa tcccctgtcc tatctcagct gaacgaccag aaaccatttg ggctccggca
     3181 caggaaagtc tgtgccggag ccttttcatg cccctttgta catgcggaat aacgggggaa
     3241 taagaaaagt cccgccgctc aggagcctga gcggcgggac ttctgatcgt tagtcagaag
     3301 gaaaggagga cccgatcaca gatggatggc ctggaccacc ttggcgcacc gggggcacag
     3361 ggtgggatgg gccgcattgg cacccaccag ggggtggtgc ttccagcagc gctcgcactt
     3421 gtctccattg gcctcgctca ccagtacccg cacaccggcg atgggggtgt cgctcccctg
     3481 ggcatacaga gccgggtcgt ccgacacctc cacgtcggag acgatgaaca ggtcggccca
     3541 ctggtcctgg aacttctcct gaagggcggt caggttgtcc gcctggtcct gggcccgcac
     3601 cagagtgatg tgggcctcca ggctcttgcc gatcttcttc tcgttccgcg cggcctccag
     3661 ggcggcgttc accccgtccc gcagctggat cagagcagtc caggagacca acgcgcccat
     3721 gtcgtagtcc tggaagggct ggttcatctc attgagaacc acattgcggg ggtcgtcacc
     3781 ctcccggtga ggcatggcct gccagatctc gtcgcaggtg aaggccagga tgggggcgaa
     3841 cagcttagta atggtgtcca ggatcatcca cagagcggtc tgagcggagc ggcgctccag
     3901 gctgtccact ccctcacagt acagacggtc cttgatgatg tccagataga aggagctgag
     3961 ctccaccaca cagaagtcgt tgatggcgtg ggagaccaca tggaactcgt agttgtcgta
     4021 ggcggcaaag cactttcgga tcagctcgtt gagcttggtc accgcccagc ggtccagctc
     4081 cagcatctcc tccggggaca ccaggtgatc ggggtcaaag ccgtccaggt tgcccaggca
     4141 gtaccgggcg gtattgcgga acttcagata gttctggctg agctgcttaa agatgttgtc
     4201 cgaacagcgc acgtccacat ggtagtcggc agaacccgcc cacagccgga gcaggtcggc
     4261 accatacttc ttaaagatct ccgcggggtc tacgccgttg cccagggact tgtgcatagc
     4321 cttgccctcg ccgtccacgg tccagccgtg ggtgacgcac tcctggaagg gagccccctt
     4381 gcccagcgcg cccacggcgg tgagcaggga ggactggaac cagccgcggt actggtccag
     4441 accctccata tacatgtcgg cgggccagaa attctggtcc ttctgcatgg aggcgaagtg
     4501 ggtggagccg gagtcgaacc agccgtccag ggtgtcctcc tccttggtga agcccgtcct
     4561 tgagccgcag tgggggcagg cgaagtcctc tggcaggata tccttggcct ccatctcata
     4621 ccaggcgttg gagcccttct caccgaagag cctgctcaca gcctcaatgg tctcgtcggt
     4681 gacgatgggc ttgccgcagt cggcacagta gaacaccggg atgggcaggc cccagcggcg
     4741 ctggcggctg atgcaccagt cggcccgctc ccggatcatg gagatcatcc ggtccttgcc
     4801 ccaggcaggg agccaccgaa catcgtcgca ggcggccacc gcctcgtcct tgaaggagtc
     4861 cacggaacag aaccactggg gggtggcccg gaagatgatg ggttttttgc agcgccagca
     4921 gtgggga
//
LOCUS       DBMDSJ010000084         4882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4762_length_4882_cov_1.802133, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000084 DBMDSJ010000000
VERSION     DBMDSJ010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_4882_cov_1.802133"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="ACZPAQ_03095"
     CDS             complement(<1..1726)
                     /locus_tag="ACZPAQ_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR0902998.1"
                     /translation="MTRLRAVLLAPPGTQPKLLRWHHVWLAFALTGLGFCVGVLTLML
                     AATAYLKLNSTDLFLSYFLLEPMLLLLNVLPPILLIWFFYFLTRRAWAAFLGSFLPCV
                     GLALVNYFKIRLRGDPLLAADLRLVTEAGGIVGHYQLDLTWLVWLCAACLILGLLFSV
                     FLLPRGIGRGPLRWFCLCSSAALLAVSYAALYCDQSLYDRTANDTHITPWSDVEVFVS
                     RGCLYPFLYSVQDMFPTPPEDYDPEEAASLLARYPDQDIPADRRVNVVGVMLEAFCDL
                     TDFPALAEQPAVQAVYAPLHDLEEQSVSGDLLTNIFAGGTVDSEWGFLTGYTQHEDFR
                     KPTDSYVWYFREQGYHTLFHHPGYSWFYNRQNVNEYLGFEESWFTENYYGALVDPVDA
                     VMRSDEALVDGILDGLREPGPWFSFSVSYQNHGPYETASAQEVFLSPGDTGWSAETCN
                     MLNNYMSNVSSTLAAMERLTRELNARPEPFVLVLFGDHKPWMGNGNTGYTEIGAGFDL
                     SQLSGFREYYSTPYLIWANSAAREALGSSFTGEGSDFSPCFLMNKVFELCGWEGPGFM
                     ELSRQLEAI"
     gene            2023..2667
                     /locus_tag="ACZPAQ_03100"
     CDS             2023..2667
                     /locus_tag="ACZPAQ_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIR0902999.1"
                     /translation="MGLRGIFDPENGFFRTLSRMVDVVGLSLLWVALSLPLVTFGPAC
                     AALYHTVTHCVACQGDSTYLTFFRSFRANLRVGIPAGLLVLAAGALLLQGYQIMVLAA
                     NSVGGGALAMYGCYCAALIVPAGVSCWAFPLLSRFQMGLGHLLVTSFQVTLRHLPQTL
                     LLILLLFVTVWTTAGFFPLAVFTPALNALLASLPAERVIRRYLPEEENGGEEEL"
     gene            complement(2739..3407)
                     /locus_tag="ACZPAQ_03105"
     CDS             complement(2739..3407)
                     /locus_tag="ACZPAQ_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR0903000.1"
                     /translation="MKRHVICLGDSNTHGYCGAQEGETLCGGRFSEAERWPCLLQRAL
                     GEEYLVIEEGLCGRTTVFPDPLEPGLDAISYLRPCLLSHAPVSLLILMLGTNDTKERF
                     GLSAEEIARGMERLARQAMSEDCWGPGGPDLLIVAPPAIRPEVEHSPVAAAMGRGCAE
                     KSRQLAEHYRRLAARLGARFLDGEGCELNRVDHMHLTQAGHAQLAARVGAVLSPPATG
                     ALGQ"
     gene            3642..>4882
                     /locus_tag="ACZPAQ_03110"
     CDS             3642..>4882
                     /locus_tag="ACZPAQ_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0903001.1"
                     /translation="MKFKKVAALVLASAMTLGLAACGNGNSSTPSQSSGGASSSGTTS
                     SSATEISLWSFNIGGFTEASNWDSLIAAFNEANPDIKVTVTPINYQDGDQKLTSAITS
                     GSGPDIIFEGPERIVGNYAREGLMVDLGDVWGDASADIGENISSVCQLDGTYYMYPLS
                     AAAHCMAINYEVFEAAGALQYIDEETRTWTTEGFEAAMAAIKTAIDNGTVTVATPGIV
                     YCGAQGGDQGTRALVNNLYSDYYVTEDGTSYNANSANNVKALTLLQNMVNAGSLSANA
                     SYAAADELQAFANQTCAVSFCWNFSNYSQYADQTQFTPFAMAFPSDDGTPELEMAGPY
                     GFGIFDNKDQAKIDAAKKFIDFVCNDATAGVEAVKTSSFFPVHSDWGDVYADAEDADL
                     RAPFALMSNYLGRYYNLTGGWT"
BASE COUNT          931 a         1597 c         1479 g          875 t
ORIGIN      
        1 agatggcctc cagctggcgg gacagctcca tgaagccggg gccctcccag ccgcacagct
       61 caaatacctt gttcatcaga aagcagggag agaagtcgct gccctccccg gtgaagctgc
      121 tgcccagcgc ctcacgggcg gcggagttgg cccagataag gtagggggtg gagtagtact
      181 cccggaagcc ggagagctga gacaggtcga agccggcccc gatctcggtg taccccgtat
      241 tgccgttgcc catccagggc ttgtggtccc caaaaaggac cagcacaaag ggctcgggcc
      301 gggcattcag ctcccgggtc agccgctcca tggcggccag ggtgctggag acattggaca
      361 tatagttgtt gagcatgttg caggtctccg ccgaccagcc ggtgtcccca ggggacagga
      421 agacctcctg ggcggaggcg gtctcatagg ggccgtggtt ctgatagctg acggagaagg
      481 agaaccaggg gcccggttcc cgcagcccgt ccagaatacc gtccaccagc gcctcgtcgg
      541 agcgcatcac ggcatccacc gggtccacca gcgccccgta gtagttctcg gtgaaccagc
      601 tctcctcaaa gcccaggtac tcgttgacat tctggcggtt gtagaaccag ctgtagccgg
      661 ggtggtgaaa aagggtgtga tagccctgct cccggaaata ccagacatag gagtcagtgg
      721 gcttgcggaa gtcctcgtgc tgagtgtagc cggtaagaaa gccccactcg ctgtccaccg
      781 tgccgcccgc gaagatattg gtcagcaggt cgccggagac ggactgctcc tcaaggtcgt
      841 ggagcggggc atagaccgcc tggacggcgg gctgctccgc cagggcgggg aagtcggtca
      901 ggtcacagaa ggcctccagc atcaccccga ccacgttgac ccggcggtcc gccgggatgt
      961 cctggtctgg ataccgggcg agcagggagg cggcctcctc cgggtcgtag tcctccggcg
     1021 gggtggggaa catgtcctgc acgctgtaca gaaaggggta gaggcagccc cgggagacaa
     1081 agacctccac atccgaccag ggggtgatat gggtgtcgtt ggcggtgcgg tcatacaggc
     1141 tctggtcgca gtagagggcc gcataggaca ccgccagcag ggcggcggag gagcacaggc
     1201 agaaccagcg aagggggccc cggcctatcc cgcggggcag aaggaaaacg gagaacagga
     1261 gacccaggat caggcaggcc gcacacagcc acaccagcca ggtcaggtcc aactgatagt
     1321 gtcccacgat cccccccgcc tcagtgacca ggcgcaggtc ggcggccagc agagggtccc
     1381 ctcgcaggcg gatcttgaag taattcacca gggccagtcc cacacagggg aggaagctgc
     1441 ccagaaaagc tgcccaggcc cggcgggtaa gaaaatagaa gaaccagatg agcaggatgg
     1501 gcggcaggac attgaggagc aggagcatgg gctccaacag gaaataggac aggaacaggt
     1561 ccgtgctgtt cagtttgaga taggcggtgg cggcgagcat cagcgtcagg acgccgacac
     1621 agaagcccag ccctgtcagg gcaaatgcca gccagacgtg gtgccagcgg agcagtttgg
     1681 gctgggtccc tgggggagcc agcagaacag cgcgcagccg agtcattcag atcacctcat
     1741 gcgggatggg ggacgcggcc ccataatgga gcagtatatc acagcggacc ggaaaaagaa
     1801 acagcggatt tcccaggcga tccagcccga tgcccgggca gccgccccct gcgtggaatg
     1861 cggggcccaa gagacgggaa ggggcggcgg ctccaagcat cccgtggggg gcccagaccc
     1921 gccccgcaaa ctcccgtctt gcctctgctg ccgttccagt ggtataatgt ttcccgacct
     1981 atcacagata cgggactatg tcccggggaa agggtgtcgc ttatgggtct cagaggtatt
     2041 tttgatccgg aaaacggctt tttccgcaca ctctcccgca tggtggacgt ggtggggctc
     2101 agccttttat gggtggcgct ctccctccct ctggtcacct tcggcccggc ctgtgccgcc
     2161 ctgtaccaca cggtgaccca ctgtgtggcc tgccaggggg acagcaccta cctcaccttc
     2221 ttccgctcct tccgggccaa tctccgggtg ggcatccccg ccggcctgct ggtcctggcc
     2281 gcgggcgccc tgctcctcca ggggtaccag atcatggtcc tggcggccaa ttccgtgggc
     2341 ggcggcgccc tggccatgta cggctgctac tgcgcggccc tcatcgtccc cgccggcgtg
     2401 tcctgctggg ccttccctct gctgtcccgc ttccagatgg ggctgggaca cctgctggtc
     2461 acctcctttc aggtgaccct gcgccacctg ccccagaccc tgctcctgat cctgctcctg
     2521 ttcgtcacgg tgtggaccac agccggcttc ttcccgctgg ccgtcttcac ccctgctctc
     2581 aacgcactgc ttgcctccct gcctgcggag cgtgtcatcc gccggtatct cccggaggag
     2641 gagaatgggg gagaggagga gctgtgaccc ctcttcccgc ctctcatccg acggcaaacg
     2701 ggcggggaca ccattcccgg tgtccccgcc cgcttttttc actgcccgag cgcccccgtg
     2761 gccggagggg agaggaccgc tcccaccctg gccgccagct gggcatggcc cgcctgggtc
     2821 aggtgcatgt ggtccactcg attcagctca cagccctccc cgtccaggaa acgcgcgccc
     2881 agcctggctg ccagccggcg gtagtgctcc gccagctgac gggacttctc cgcgcatccc
     2941 cggcccatgg cagcggccac gggggagtgc tccacctccg gacgaatggc gggaggcgcc
     3001 acgatcagca gatcgggtcc cccgggtccc cagcagtcct ctgacatggc ctgccgggcc
     3061 agccgctcca tcccacgggc gatctcctcc gccgagaggc cgaagcgctc cttggtgtcg
     3121 ttggtaccca gcatcaggat cagcagggag accggggcat ggctcagcag gcagggacgc
     3181 agataggaaa tggcgtccag ccctggctcc aggggatcgg ggaataccgt ggtccgcccg
     3241 cacagtccct cctcgatcac cagatattcc tctcccaggg cgcgctggag cagacagggc
     3301 cagcgttccg cctcgctgaa ccggcctccg cagagggttt ccccctcctg agctccacag
     3361 tagccgtgag tattggagtc gcccagacag ataacatggc gcttcatgat atttcagtcc
     3421 tccgttttgt ttattttttt gtacctataa cctatcagtt ttcctgaaat tgtcaaggtt
     3481 ttgtttcaat ttgggtctaa ctccacgtta aaaattttct tttttcgcaa tttgtgaaaa
     3541 ttttttcatt ccgtcttgac aagctcggtg atttcctata aaattttctt aacccaatcg
     3601 gcaattattt gtgaaaaatg attcacaagg aggacaatga aatgaaattc aaaaaagtag
     3661 cagctcttgt cttggcttcc gccatgacgc tgggcctggc tgcctgcggc aacggcaaca
     3721 gctccacccc cagtcagtcc agcggcggtg cctcttcgtc gggcaccacc tccagcagcg
     3781 ccactgagat cagcctgtgg tccttcaaca tcggcggctt caccgaggca tctaactggg
     3841 attccctgat cgccgccttc aacgaggcca accccgacat caaggtcacc gtcaccccca
     3901 tcaactatca ggacggcgac cagaagctca cctccgccat cacctccggc agcggccccg
     3961 acatcatctt tgagggcccc gagcgcatcg tgggcaacta cgcccgtgag ggcctgatgg
     4021 tggacctggg cgacgtgtgg ggcgatgcct ccgcggatat tggcgagaat atttccagcg
     4081 tctgccagct ggacgggacc tactacatgt accccctgag cgcggccgcc cactgcatgg
     4141 ccatcaacta cgaggtcttt gaggctgccg gcgccctgca gtacatcgat gaggagaccc
     4201 gcacctggac caccgagggc ttcgaggccg ccatggctgc catcaagact gccatcgaca
     4261 acggcaccgt caccgtggcc acccccggca tcgtctactg cggcgcccag ggcggtgacc
     4321 agggtacccg cgccctggtg aacaacctgt actccgacta ctatgtcacc gaggacggca
     4381 cttcctacaa tgccaacagc gccaacaatg tgaaggctct gaccctgctg cagaacatgg
     4441 tgaacgccgg ctctctgtcc gccaacgcca gctacgccgc tgccgatgag cttcaggctt
     4501 tcgccaacca gacctgtgcg gtcagcttct gctggaactt ctccaactac agccagtacg
     4561 cggaccagac ccagttcacc cccttcgcca tggcgttccc ctctgacgat ggcacccccg
     4621 agctggagat ggccggcccc tacggcttcg gtatcttcga caacaaggac caggctaaga
     4681 tcgacgccgc caagaagttc atcgactttg tctgcaacga tgccaccgcc ggtgtggagg
     4741 ctgtgaagac ctccagcttc ttccccgtgc actccgactg gggcgatgtc tacgccgacg
     4801 ctgaggatgc cgacctgcgc gctcccttcg ccctgatgag caactatctg ggccgctact
     4861 acaacctgac cggcggctgg ac
//
LOCUS       DBMDSJ010000085         4856 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4796_length_4856_cov_1.714616, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000085 DBMDSJ010000000
VERSION     DBMDSJ010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4796_length_4856_cov_1.714616"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="ACZPAQ_03115"
     CDS             <1..618
                     /locus_tag="ACZPAQ_03115"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR0903002.1"
                     /translation="PAPGHSDGTLVNALLYHCGNTLSGINGTLRPGIVHRIDRDTSGL
                     IIAAKNDRAHLSLAAQLQDHSLSRVYEAVAVGGLREDCGTVDAPIGRHPVDRKRMAVD
                     RQNGREAVTHWRVLARYPGFTHVECRLETGRTHQIRVHLASIGHPLLGDVVYGSKKPV
                     PGLAGQCLHARRLRFTHPSTGQLVELECPLPGWFQDVLDRLSRLN"
     gene            701..1135
                     /locus_tag="ACZPAQ_03120"
     CDS             701..1135
                     /locus_tag="ACZPAQ_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903003.1"
                     /translation="MVETGVPGLEQTGWDALRGQLNTLDTSLLFLALLTGATALSWAI
                     LSRQRGALLSLLSGEDIPEPDLIPARLAVSALVVGSLSYFFLLSLETLCTARSQGAAA
                     CASAGRNARASLLVLAAALIRLYDLNILRCTAPALESEELPA"
     gene            complement(1202..2206)
                     /locus_tag="ACZPAQ_03125"
     CDS             complement(1202..2206)
                     /locus_tag="ACZPAQ_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR0903004.1"
                     /translation="MLYGILVALSAVIVGVYAVWNLTVRPPEIPQEPDPAPPAGSGSQ
                     AAEPVEQEQPAGRQRREDVYNFVLLGRDRESANTDTIIVVSYDVANQKVGMISIPRDT
                     AVNRDWRKDPKINGAYAGAGVDVLKEEIEQTFGIPIDYYIYVDLKGFVALVDELEGVD
                     VYIPEEMNYDDPYQDLHIHYTKGQHHLNGQQAMEVVRFRHNNDGSGYTDVGRAEMQRQ
                     VLVALAKKVVSWNSLTKVQEFVEIFQEYVKTDLSTTDMLYFASQAVGVDLDTGITQGT
                     LEGRGEGVVRGYKYCFVFQAEDILPTLNELVNPYDQPLTEEDLDLPQAEYYWNGTVID
                     "
     gene            complement(2391..3440)
                     /locus_tag="ACZPAQ_03130"
     CDS             complement(2391..3440)
                     /locus_tag="ACZPAQ_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903005.1"
                     /translation="MKKWIVPAALVLMLALAACAPACVSERAPAGGESSGAGETTGTQ
                     VLSDGEKALEELAEPPALTVSCGWDQITLSWSGNASWSKAGPDGEGETWIACGLHTLD
                     SQEYPVLYTGIPAGAEQTEGGGVASLAPALTLAFEVQPTQVTLRRWPAEYVGRTQEGE
                     DQAETLEAENGTVLLPGDGDCVYEICAQWGDVGSASYVFRTRPQTRPEPLTGLAELYC
                     RALRDLWETDPGLNSGAELLAFDWTGCTGLTEREQEGVMETLGAELGLDTRRGTLEEL
                     AEEGLIRADPDSGFEEFPAGLLLTVEDSIEADGRHTFSLQKYRSSLGAYCFYDCTARQ
                     EGDGWSYAVGAEMIS"
     gene            complement(3458..4366)
                     /locus_tag="ACZPAQ_03135"
     CDS             complement(3458..4366)
                     /locus_tag="ACZPAQ_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903006.1"
                     /translation="MNQYKKYMDRQGVSPSLHARLTALAEGDGPAPAGRAPHRYRWGI
                     LAACCVLAAGLAWAGGVRQPDGAAQAYSMDSAGMQSAQSGAAHQGSSEAMEEDEKQLS
                     PPVWAVNYPDIGTLYRGEEGLGAALTDQAPAAETRSRALTPEELTALFREDLLRELGW
                     DCGQLGGEALYDASGALLRVTVRGGGVGEEAQTSFALTLAPGDLPSPGEAVPGGTVTE
                     VNGTEVTAWRLTGDLDRDGRAEGLCHSQFLTGDMWVVLETESRASAGAEEPWEEAARW
                     SDSFLSAVLQGEGDALTLDHLLEAEP"
     gene            complement(4363..>4856)
                     /locus_tag="ACZPAQ_03140"
     CDS             complement(4363..>4856)
                     /locus_tag="ACZPAQ_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903007.1"
                     /translation="RGEQGMSQISNRPVDWETLVKENEDRLYRAALAILGDAQEAEDA
                     VQDTFLKFLEKAPAELDSPPAWLMRVLVNGCRDRLRAAWRRRSVPLWESLPAPEPEER
                     QELEEIFSLPPADRAVIHLFYYEGLSTGEIARLTGQAEGTVRSRLSRARARLRRLLEG
                     ETK"
BASE COUNT          716 a         1793 c         1435 g          912 t
ORIGIN      
        1 cccgcccccg gccattcgga cggcacgctg gtcaatgccc tgctatatca ctgtggaaat
       61 accctctctg ggatcaacgg caccctgcgc cccggcatcg tccaccgcat cgaccgggac
      121 accagcggcc tgatcatcgc cgcgaagaac gaccgggccc acctgtccct ggccgcccag
      181 ctccaggacc acagcctgtc ccgggtctat gaggcggtgg cggtgggcgg gctgcgggag
      241 gattgcggca ccgtggacgc ccccatcggc cgacaccctg tggaccggaa gaggatggcc
      301 gtcgaccggc agaacggccg ggaggcggtg acccactggc gggtgctggc gcgctacccg
      361 ggcttcaccc atgtggagtg ccgcctggag acgggacgga cccaccagat ccgggtccac
      421 ctggcctcca tcggccaccc tctgctgggc gatgtggtct acggcagcaa aaagccggtg
      481 cccggcctgg ccggccagtg cctgcacgcc cggcggctgc ggttcaccca tccctccact
      541 ggacagcttg tggagctgga gtgtcccctc cccggctggt tccaggatgt actggaccgg
      601 ctctcccgcc tgaattgact gcgcctcccc ggtccttcag ccagggaggc gttttttcac
      661 ccgctcccca tgggcggcat aggatagagg gctgtaaaac atggtggaaa cgggggtgcc
      721 gggcttggag cagacgggat gggacgccct gcggggccag ctgaacaccc tggacacctc
      781 tctgctgttc ctggctctcc tcaccggggc caccgccctc tcctgggcca tcctctcccg
      841 gcagcgcgga gcccttctct ccctcctctc tggagaggac atcccggagc cggacctgat
      901 tccggcccgg ctggccgtct ccgccctggt ggttggctcc ctgagctact tttttctgct
      961 gtccctggag actctctgca ccgctcgatc ccagggggcg gcagcctgtg cctcggccgg
     1021 gcgcaatgcc cgtgcctccc tgctggtgct ggcggctgct ctgatccggc tgtatgacct
     1081 caacatcctc cgatgcaccg ctcccgccct ggagtcagaa gagctccctg cctagcggac
     1141 cgccgcaata gaaaacaccg cgcctcctcg gctctgggcc ggagggcgcg gtgttctcgt
     1201 ttcagtcaat gacagtgccg ttccagtagt actccgcctg gggcagatcc agatcctcct
     1261 cggtcagcgg ctggtcgtag gggttgacca gctcgttcag ggtgggcagg atgtcctccg
     1321 cctggaagac aaagcagtat ttgtatcccc ggaccacgcc ctcgccccgg ccctccagag
     1381 tgccctgggt gatgccggta tccaggtcca ctcccactgc ctggctggcg aagtagagca
     1441 tgtcggtggt ggacaggtct gtcttgacat actcctggaa gatctccacg aactcctgca
     1501 ccttggtcag ggagttccag gagaccactt tcttggccag ggccaccagc acctggcgct
     1561 gcatctccgc ccggcccacg tcggtgtaac cgctcccgtc gttgttgtgg cggaagcgga
     1621 ccacctccat ggcctgctga ccgttcaggt ggtgctggcc cttggtgtag tggatgtgca
     1681 ggtcctgata ggggtcgtca tagttcatct cctccgggat atagacgtcc accccctcca
     1741 gctcgtccac cagggccacg aatcccttca ggtccacata gatgtagtag tcgatgggga
     1801 tgccgaaggt ctgctcgatc tcctccttca gcacgtccac ccccgccccc gcgtaggctc
     1861 cgttgatctt ggggtccttt ctccagtccc ggtttaccgc cgtatcccgg ggtatggaga
     1921 tcatccccac cttctggttg gctacatcgt agctgaccac gatgatggtg tcggtgttgg
     1981 cgctctccct gtcccggccc agaaggacga aattgtacac atcctcccgc ctctgccgtc
     2041 ctgcgggctg ctcctgttcc accggctccg ccgcctggct cccgctgccg gcgggggggg
     2101 ccgggtcggg ctcctggggg atctcaggcg gccggaccgt caggttccac accgcgtaca
     2161 cccccacgat cacggcggat aaagccacca gtattccgta gagcaccatt ccgatccgaa
     2221 aaagcgggct gcgctttcgg cgctttcctc cccgctgtgt ctgtatgcgt gccagagctg
     2281 ttcccctctt tcctgtttcg gcaatatacc tgtccagtat aaccagttcg cctcgattcg
     2341 gcaagaggga ctttttctct tttaaaaatt ttaaaaattg gttcctccct tcaggagatc
     2401 atctccgccc ccacggcata gctccagccg tccccctcct gccgggcggt gcagtcatag
     2461 aaacagtagg cccccaggct ggagcggtat ttctggagcg aaaaggtgtg tctcccgtcc
     2521 gcctcgatgc tgtcctccac ggtgagcagc agcccggcgg ggaactcctc aaagccgctg
     2581 tccgggtccg cccggatcag accctcctcc gccagctcct ccagggtgcc ccggcgggtg
     2641 tccagcccaa gttcggcacc taaggtctcc atgaccccct cctgctcccg ctccgtcaaa
     2701 ccggtgcagc cggtccagtc aaaggccagc agctccgcgc cgctgttcag gcccggatca
     2761 gtctcccaca ggtccctcaa ggcccggcag tacagctccg ccaggccagt caggggctcc
     2821 ggccgggtct gtggtcgggt gcggaacaca tagctggctg agcccacatc accccactgg
     2881 gcgcagatct catacacgca gtccccgtcc cccgggagca gaacggtccc gttctctgcc
     2941 tccagggtct ccgcctggtc ctccccctcc tgggtccgcc ccacatattc agcgggccag
     3001 cggcggaggg tcacctgtgt gggctggacc tcgaaggcca gcgtcagcgc cggggcgagg
     3061 gaggccaccc ctcccccctc tgtctgttcc gcccccgcgg ggatgccggt atagaggacc
     3121 gggtattcct gactgtccag ggtgtggagg ccgcaggcga tccaggtctc cccctcccca
     3181 tccggtcccg ctttgctcca gctggcgttc cccgaccagg acagggtgat ctggtcccag
     3241 ccgcaggata cggtgagcgc cggcggctcc gccagctctt ccagggcctt ctccccatcg
     3301 gagaggacct gggtcccggt ggtctccccc gctccgctgc tctctccccc cgccggggcc
     3361 cgctctgaaa cgcaggccgg agcgcaggca gccagcgcca gcatcaacac cagcgccgcc
     3421 ggaacgatcc attttttcat ctctctccgc tcctttctta cggctccgcc tccagcagat
     3481 ggtccagcgt cagggcgtct ccttccccct gcagaacggc ggagaggaag ctgtcactcc
     3541 atcgcgcggc ctcctcccag ggctcctccg ccccggcaga cgcccggctc tcagtctcca
     3601 ggacgaccca catgtctccg gtcagaaact ggctgtgaca cagcccctct gccctgccgt
     3661 ctctgtccag gtcccctgtc agtcgccagg cagtgacctc tgtcccgttc acctccgtca
     3721 ctgtcccgcc ggggacggcc tcccctgggg agggcaggtc tcccggagcc agggtgaggg
     3781 cgaaagaggt ctgcgcctcc tccccgactc cgcccccccg tactgtgacc cggagcaggg
     3841 cccccgaagc gtcatacagg gcctctcccc ccagctggcc gcagtcccag cccagctccc
     3901 gaagcagatc ctcccgaaag agggcggtca gctcctctgg agtcagcgcc cggctccgtg
     3961 tctccgctgc cggggcctgg tctgtcaagg ctgcccccag gccctcctcc ccccggtaca
     4021 gcgtcccaat gtccgggtag ttcaccgccc acacaggggg ggagagctgc ttttcatcct
     4081 cctccatggc ctcggagctc ccctggtgcg cggccccgct ctgggcggac tgcatcccgg
     4141 cactgtccat gctgtaagcc tgggcggccc catccggctg ccggacgccg ccggcccagg
     4201 ccagtcctgc tgccagcaca cagcaggcag ccagtattcc ccaccggtac cggtggggag
     4261 cccgccccgc aggggcgggc ccgtcaccct ccgccagcgc tgtcagccgg gcgtggagcg
     4321 acggggatac cccctgccgg tccatatact ttttgtattg attcattttg tttctccttc
     4381 tagcaatctc ctgagccggg cccgggcccg ggagagccgg gatcggacgg tcccctccgc
     4441 ctgtcctgtg agccgggcga tctctccggt ggagagtccc tcgtagtaaa acagatggat
     4501 gacagcccgg tctgccggcg ggagggaaaa gatctcctcc agctcctgcc gctcctccgg
     4561 ctctggggcg gggagggact cccacagggg gacgcttcgc cgccgccagg cggcccgcag
     4621 ccggtcccgg cacccgttca ccagcacccg catcagccag gcgggaggac tgtccaactc
     4681 cgccggagcc ttttccagaa attttagaaa ggtgtcctgc accgcgtcct ccgcctcctg
     4741 ggcgtcccca aggatggcca gcgccgcccg gtacaggcgg tcctcattct ctttcaccag
     4801 cgtctcccaa tccaccggcc ggttggatat ctgagacatc ccctgctccc cccttt
//
LOCUS       DBMDSJ010000086         4812 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4851_length_4812_cov_1.855657, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000086 DBMDSJ010000000
VERSION     DBMDSJ010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4851_length_4812_cov_1.855657"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="ACZPAQ_03145"
     CDS             complement(<1..473)
                     /locus_tag="ACZPAQ_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR0903008.1"
                     /translation="MLTPQEVSERAFPKASFGGYNMAQVDEFLDLLTADYTSLYNENA
                     VLKSKMKVLVEKVEEYRATEDAMRKALMTAQRMADELVKEAEAKKAQILSDAEGEAKE
                     RVANIRKQAGDEEFRLSAAQSATAAYVAKVRALHEQELRYLDGLSQLCPDAPVSQA"
     gene            complement(505..1374)
                     /locus_tag="ACZPAQ_03150"
     CDS             complement(505..1374)
                     /locus_tag="ACZPAQ_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903009.1"
                     /translation="MGRVAVRPPGRLLPLLLLLALLSGCAGGGAASASEDEEPQAPLL
                     MEHGCLSMDLDGDGAEEAVRLYALQAADDLPPEALPPEGSDSRNLLFRWYVEAQIGDD
                     LVTCDLNGRYYYYSPQQDQPLFFSIQDRQGASLVVAGLIPQSNGAGAGMLEVHVLSYD
                     KGAFQVLELPLWSIQSVQAGMTAQITVPETGTVETLDLLQWLELRGEGPLYEADGTLA
                     WPCAPASWEEGDIYALLPAEEGIWTAQRLYGQALSDLMGYLVTHLTWDDGTPVVLEQY
                     FDWRAQNDDEVLR"
     gene            complement(1379..1861)
                     /locus_tag="ACZPAQ_03155"
     CDS             complement(1379..1861)
                     /locus_tag="ACZPAQ_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR0903010.1"
                     /translation="MGFMDELKRLAHPYEDEDEDDFEEFEPAPRAAVPERRDRSERMD
                     RTGSLYSAPVEPERRSNKVVNIHTTTQLQVVLVKPERFENASEIADHLREKRTVVLNL
                     ESTNKEIARRLLDFLSGVAYANEGKIKKVAISTYIITPYNVDILGDLIDELENNGLYF
                     "
     gene            complement(1905..2615)
                     /locus_tag="ACZPAQ_03160"
     CDS             complement(1905..2615)
                     /locus_tag="ACZPAQ_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR0903011.1"
                     /translation="MSIQQQVQAVRARMAAAAESSGRRPEDIALCAATKVQSDDTIRA
                     AIAAGITLCGENRVQELTAHLAAGAYEGARVHFIGHLQTNKVKQVVGRVDLIQSVGSE
                     HLLRAVEAQADKLGLVQDILLEVNIGMEESKGGVDPAQLRPLAELAQSLPHVRLRGLM
                     AIPPISPLPGANRPFFAKMYQLFVDIRREMSDNQCDIDCLSMGMSGDFEDAIAEGATL
                     VRVGTALFGPRPPMHTAR"
     gene            complement(2667..3935)
                     /locus_tag="ACZPAQ_03165"
     CDS             complement(2667..3935)
                     /locus_tag="ACZPAQ_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024112.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR0903012.1"
                     /translation="MKQGNSLVTLVIVLLAVMLAAYLGVYAWDSFTDPFTTTLTYSYT
                     ANDSLEADGWVVREEQLLPAQSGILDITRAEGEEVGRGQTVALVHQNSQALDVQAQME
                     ELAMEIELLDYAMNQTDDVVSAARLDESILQSLASLRFASASGSYRQLDDDVMELKSQ
                     VLKRSYTYGEGLDSSQLSALRQSLIEEYRALRTQSSSVTSRITAPAAGVFSSLADGYE
                     SLLTPQSILTMTPADLDALAGQQVTAPSGTAGKLITSDRWYFAAAVSEEEALRLSKES
                     SVTARFSGSFSQDVPMTLEQVGEAVDGRAVVVLSSNRYLSQITLLRGQTVELIFGSSS
                     GLRIPKAALRMVTQESTDPETGETVQTNLVGVYTLVGGRAEFKPVEILTEESDFYVVS
                     PATEGRAVLRAGDEVIVRATGLYDGKLLNF"
     gene            complement(3987..4223)
                     /gene="hfq"
                     /locus_tag="ACZPAQ_03170"
     CDS             complement(3987..4223)
                     /gene="hfq"
                     /locus_tag="ACZPAQ_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR0903013.1"
                     /translation="MQKTNNLQEIFLTQVRRDRRPVTIFLMNGFQMRGYVTGFDAFTV
                     VLTSDGKQQVIYKHAISTIVPERPVPLCQPGELE"
     gene            complement(4276..>4812)
                     /locus_tag="ACZPAQ_03175"
     CDS             complement(4276..>4812)
                     /locus_tag="ACZPAQ_03175"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR0903014.1"
                     /translation="EAMLGELAAADPEAAARLHPNDRKRIVRALEVFQQTGKTISQHN
                     RETRALPLRYDALTLSLAFQRREDMWARIDRRVDEMMAAGLAEEVRALLDSGVPVRCT
                     AMQAIGYKEMAAALRGGGDVCAAAEEIKLRSRQYAKRQLTWFRRTEGAKWLFWGPEPD
                     FEAACRTSTKYLEEFGLV"
BASE COUNT          834 a         1508 c         1595 g          875 t
ORIGIN      
        1 gcctgggaga ccggggcgtc ggggcacagc tgggaaaggc cgtccaggta gcgcagctcc
       61 tgctcgtgga gggcccgcac cttggccaca taggcagcgg tggcggattg ggcggcggac
      121 agacggaact cctcgtcgcc ggcctgcttg cggatattgg ccacccgctc cttggcctcc
      181 ccttcggcgt cggagaggat ctgggccttt ttggcctccg cctccttcac cagctcgtcg
      241 gccatccgct gggcggtcat cagcgccttg cgcatggcgt cctcggtggc gcggtactcc
      301 tccaccttct ccaccaggac cttcatcttg ctcttcaaaa cggcgttctc attgtacagt
      361 gaggtatagt ccgctgtgag cagatccaga aattcatcca cctgagccat gttgtagccg
      421 ccgaaggaag ccttgggaaa ggcgcgctcg gagacctcct gaggagtgag catattcatt
      481 acctcctaaa tcaaaacgct tttttcagcg caggacctcg tcgtcgttct gggcgcgcca
      541 gtcaaaatac tgctccagga ccacgggagt gccgtcgtcc caggtgaggt gggtcaccag
      601 atagcccatc aagtccgaca gggcctgtcc gtacagccgc tgggccgtcc agataccctc
      661 ttcggctggg agcagggcat agatatcccc ctcctcccag gaggcggggg cgcagggcca
      721 ggcgagagtc ccgtccgcct catagagcgg tccctctccc cgcagctcca gccattggag
      781 caggtccagc gtctccacgg tgccggtctc tggtacggtg atctgggcgg tcatgccggc
      841 ctgaacgctc tggatagacc agagggggag ctccaggacc tgaaaagctc ccttgtcata
      901 ggagaggaca tgcacttcca gcatgccggc cccggcccca ttggactgtg ggatcagacc
      961 ggccaccacc agggacgcgc cctgccggtc ctgaatggag aagaacaggg gctgatcctg
     1021 ctggggagaa tagtaataat accggccatt caggtcacag gtgaccaagt catccccaat
     1081 ctgggcctct acataccagc ggaacagcag attccgggaa tccgagccct ccggcggcag
     1141 cgcctcaggg ggcaggtcgt ccgcggcctg aagggcatac aggcggacgg cctcctctgc
     1201 gccgtctccg tccagatcca tggagaggca gccgtgctcc atgaggagcg gggcctgcgg
     1261 ctcctcgtct tcggaggcgg aggcggcccc tccgccggcg cagccggaca gcagggccag
     1321 gagcaaaagg agggggagga gccggcccgg cggccgaacg gcgacgcgtc ccatctgctc
     1381 agaagtacag cccgttgttc tccagctcgt cgatcagatc ccccaggatg tccacattgt
     1441 agggggtgat gatataggtg gagatggcca ccttcttgat cttgccctcg ttggcatagg
     1501 ccacgccgga aagaaaatcc agcaggcggc gggcgatctc cttgttggtg gactccaggt
     1561 tgagcaccac ggtgcgcttc tcccgcagat ggtcggcgat ctcgctggcg ttctcaaagc
     1621 gctctggctt taccagcacc acctggagct gagtagtggt gtgaatattg acgaccttgt
     1681 tgctgcggcg ctccggctcg accggggcgc tgtacaaaga gcccgtgcgg tccatccgct
     1741 cggagcggtc ccggcgctca gggacggcag cacgaggcgc cggctcaaac tcctcgaaat
     1801 cgtcctcgtc ctcatcctcg taggggtggg ccaagcgctt gagttcatcc ataaatccca
     1861 tagcggtatc ctctcttttc cttggtgtaa gctctgccgc cggttcaacg ggcggtgtgc
     1921 atgggcggcc gggggccgaa cagcgcggtg ccgacccgga ccagcgtggc cccctcggcg
     1981 atggcgtcct caaagtcgcc gctcattccc atggacaggc aatcgatatc acattgatta
     2041 tcggacattt cgcgtcttat gtcaacaaaa agctgataca tttttgcaaa aaaaggccga
     2101 ttggcaccgg ggagggggct gataggcggg atggccatca aaccccgcag gcgcacatgg
     2161 ggcagggact gagccagctc agccaaggga cggagctggg ccgggtccac cccgcccttg
     2221 ctctcctcca tgccgatatt tacctccagc aggatgtcct gaaccaggcc cagcttgtcc
     2281 gcctgggcct ccacagcccg gagcagatgc tccgacccca ccgactggat caggtccacc
     2341 cgtcccacca cctgcttgac cttgttggtc tggaggtggc cgataaaatg gacccgggcg
     2401 ccctcatagg cgccggcggc caggtgggcg gtgagttctt ggacccggtt ctctccgcac
     2461 agggtgatgc cggcggcgat ggcggcgcgg atggtgtcgt cgctctgcac cttggtggcg
     2521 gcgcacaggg cgatatcctc cgggcggcgg ccggacgact ctgccgcggc cgccatgcgg
     2581 gcccggactg cctggacctg ctgttggatg gacatatgca atctctctct ttctctacag
     2641 gtgacgggct gccggatggg cggccctcag aaattcagca atttcccgtc atagagcccg
     2701 gtggcccgga cgatgacctc gtcccccgcc cgcaggacgg cccgcccctc ggtggcgggg
     2761 gagaccacat agaagtcgga ctcctcggtg aggatctcca ccggcttgaa ttctgcccgg
     2821 ccgcccacca gggtatagac ccccaccagg ttggtctgga cggtctcgcc ggtctccgga
     2881 tcggtggact cctgggtgac catgcgcaga gcggccttgg ggatgcgcag gccggagctg
     2941 ctgccgaaga tgagctccac tgtctggccg cggagcagag tgatctggga caaatagcgg
     3001 ttggaggaga ggaccaccac cgcacggccg tccaccgcct cccccacctg ctccagcgtc
     3061 atggggacgt cctggctgaa ggaaccggaa aagcgggcgg tgacggagct ctccttggac
     3121 aggcgcaggg cctcctcctc cgagaccgcg gccgcgaagt accagcggtc ggaggtgatg
     3181 agcttgccgg cggtgccgga gggggccgtg acctgctgcc cggccagggc atccaggtcg
     3241 gcgggggtca tggtcaggat ggactgaggg gtgagcaggc tctcatagcc gtcggccaaa
     3301 gaggagaaca cccccgcggc aggagcggtg atgcggctgg tgacgctgct ggactgggtg
     3361 cgcagggcgc ggtactcctc tatcagcgac tgccgcaggg cggagagctg ggaggagtca
     3421 agcccctcac cataggtgta gctgcgcttg agcacctggc ttttcagctc catcacgtcg
     3481 tcgtccagct gacggtagga gccagaggcg gaggcgaacc gcagggaggc cagggactgg
     3541 aggatggatt cgtccagccg ggcggcggag acgacgtcgt cggtctggtt catggcgtaa
     3601 tccagcagct cgatctccat ggccagctcc tccatctgag cctggacgtc cagcgcctgg
     3661 ctgttctggt ggaccagagc cacagtctgg ccccggccca cttcctcccc ttctgcccgg
     3721 gtaatgtcca ggatgcccga ttgggcgggg aggagctgtt cctcccgcac cacccagccg
     3781 tctgcctcca ggctgtcatt ggcagtatag gagtaggtga gggtagtggt gaagggatcg
     3841 gtaaagctgt cccaggcgta gacgcccaga taggcggcca gcatcaccgc cagaaggacg
     3901 atgaccagcg tgaccagact gttgccctgt ttcatgtgac cggctctctt tctcttttgc
     3961 ggatgggtgt ggggatgggg cgggagttac tccagctcgc cgggctggca gagggggacg
     4021 ggccgctccg ggacgatagt ggagatggcg tgcttgtaaa tcacctgctg cttgccatcg
     4081 ctggtgagga ccacggtgaa ggcgtcaaag ccggtgacat agccccgcat ctgaaacccg
     4141 ttcatcagaa agatggtgac ggggcggcgg tcccggcgga cctgggtgag gaagatctcc
     4201 tgaagattgt tggttttctg catggtgagc actccttttt tctttaaggc ggagcagagc
     4261 tcggcccgag tgcctctata ctaaaccaaa ttcttccaaa tattttgtcg aagtccggca
     4321 ggccgcttca aaatcaggct ccggtcccca gaagagccat ttggcccctt cggtacggcg
     4381 gaaccaggtg agctggcgct tggcgtactg ccgggagcgg agcttgatct cctccgcggc
     4441 ggcgcagaca tctccgccgc cgcgcagcgc ggcggccatc tccttgtagc cgatggcctg
     4501 catggcggtg caccgcaccg ggacaccgga gtccaggagg gcccggacct cctccgccag
     4561 gcctgcggcc atcatctcgt ccacccgccg gtcgatgcgc gcccacatgt cctcccggcg
     4621 ctgaaaggcc agggagaggg tgagggcatc gtaccggagc gggagggccc gggtctcccg
     4681 gttatgctgg gagatggtct ttccggtctg ctgaaagacc tccagggccc ggacgatgcg
     4741 cttgcggtcg ttggggtgga ggcgggcggc ggcctccggg tcggcggcgg ccagctcccc
     4801 cagcatggcc tc
//
LOCUS       DBMDSJ010000087         4741 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4925_length_4741_cov_1.407543, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000087 DBMDSJ010000000
VERSION     DBMDSJ010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4925_length_4741_cov_1.407543"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="ACZPAQ_03180"
     CDS             <1..345
                     /locus_tag="ACZPAQ_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid family protein"
                     /protein_id="HIR0903015.1"
                     /translation="AIQSGEAAKFLWNPEGGLGNFDTQRVLNKPVLFEDSCSALGSKG
                     DILLVDPYMYILLTKGTAKQDWSIHVEFLTDQNCFRMVFRCNGAPKVSKPLTIKNSTK
                     TRSPFVALADRK"
     gene            358..771
                     /locus_tag="ACZPAQ_03185"
     CDS             358..771
                     /locus_tag="ACZPAQ_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903016.1"
                     /translation="MKRIFEKLAFANVFAPQSVEASTEKTTGYVDASGTEEVAFLISA
                     AALGKGKSLTVTLMAADDASGSGAKAVGEAAVFTDPVGTEPQVVAVSYQVSPLNGRYV
                     AVKLQHDAAAAVVCGVLAAADGMYLPPANGMTLVV"
     gene            773..1075
                     /locus_tag="ACZPAQ_03190"
     CDS             773..1075
                     /locus_tag="ACZPAQ_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIR0903017.1"
                     /translation="MAIPEELKTAVFAYCREVEDEETLPAMTVAWDAAVGYLEGAGVT
                     RPAPESTRHGLWLGVVLPLTLDAYDQREAQTEGQNKLQDNPVLRRKLTQLKLTEGI"
     gene            1404..1805
                     /locus_tag="ACZPAQ_03195"
     CDS             1404..1805
                     /locus_tag="ACZPAQ_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HIR0903018.1"
                     /translation="MSFRRKAEAKDMEATTTMVSASGLRARITIYRRKETENELEEKT
                     FTYQPERTVWAEIVPTSGRTAPLEGAVERAEITHRVRLRISACPVIERGMYFTFRRQR
                     YDVLYGYSIYNRRGWLELYCRLVVEDSVPGI"
     gene            1789..2202
                     /locus_tag="ACZPAQ_03200"
     CDS             1789..2202
                     /locus_tag="ACZPAQ_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HIR0903019.1"
                     /translation="MSQGFDLSELQKYAKDLATVGQEYEKAERRFLQKEGSKLRSKTK
                     AQARKLGKKTGNYLKSIKRGKVYKYRGDKAVRVYSYAPHAHLIEEGHRMVTHDGREVG
                     FVKGHHVFEVAAKGFESEFYTDLDDFLSGEVVEKL"
     gene            2199..2651
                     /locus_tag="ACZPAQ_03205"
     CDS             2199..2651
                     /locus_tag="ACZPAQ_03205"
                     /inference="COORDINATES: protein motif:HMM:NF044614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator family protein"
                     /protein_id="HIR0903020.1"
                     /translation="MTLKELNKAVCGLVKRAAEASGLGAELMAEDLSAPIQRPSLKVE
                     LEEDRDARAIRHRVEQAITFRVYFFAADQYRPKLDNLAMRQALAEAFRDGVPVGEETV
                     PIDEGLSFTVTDGVLVASMDLTLDQRIIPAEEEDAELMEELNVNLEVH"
     gene            2653..3738
                     /locus_tag="ACZPAQ_03210"
     CDS             2653..3738
                     /locus_tag="ACZPAQ_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HIR0903021.1"
                     /translation="MAVTLPKIEVTFKQLAASFVRRSERGIAVLILRDATEGSGESFF
                     RFGVATQVPENEFTAANQQYIKDALSFGPLRVSVVKVGAEGTLAQALAILVQKEQTGW
                     ITVCDGTSSDWTDLVSWIKAREKEEKSWKAVCYDTTSPDCMHVVNFVNKTVTFADDRG
                     AVGGGKYTPSLAGLLAACNVLRGATGYVCSNLTTVEEPEDAETAVGSGKFVLVNEDDE
                     VQVGVDVNSMTTVNGSTQTEDMKYIETVEAMDLIRDDITATFRKDYRGKYKNTTANQM
                     LFLAVVNGYFRDLGDENVLDPTYDNTAAVDVTSQRNAWIDSGKAEAVDWDDGKVIATP
                     YKRKVFLGGDVKILGSMTDLKFVVTLM"
     gene            3754..4179
                     /locus_tag="ACZPAQ_03215"
     CDS             3754..4179
                     /locus_tag="ACZPAQ_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR0903022.1"
                     /translation="MVKYQEVLHGSTSELYINGEREELVTKIEIKMTGDFEDSSFCGE
                     YGTFPIYNGYAIEGTISGKKRNSTLENAIVEGYRTGEMPDITVISALGRAGSSARERW
                     SVSGVVFTEAALANIEAKKAVERELPFKAVLAKNLEAIE"
     gene            4179..4628
                     /locus_tag="ACZPAQ_03220"
     CDS             4179..4628
                     /locus_tag="ACZPAQ_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903023.1"
                     /translation="MARKFSFDELLAKREQREADKLKVGLLELPGTGKGLEARMPGQK
                     TVLDLYGELVAAQDARESLQCGNHALYACCPQLWDSRLQEELGCKEDPMQVLDKLFSL
                     AEQDQLGGQALRFLGLLPEPPQKTDGNDGEEKEEAQGAAPGIETVKN"
BASE COUNT          965 a         1310 c         1716 g          750 t
ORIGIN      
        1 gccatccaga gcggcgaggc ggccaagttc ctgtggaacc ccgagggagg cctgggcaac
       61 ttcgataccc agcgggtgct caacaagccg gtgctgtttg aggacagctg ctccgccctg
      121 ggcagcaagg gcgacatcct gctggtggac ccctatatgt acatcctgct gaccaagggt
      181 acggcgaaac aggactggtc catccacgtg gagttcttga cggaccagaa ctgcttccgc
      241 atggtgttcc ggtgcaacgg cgcacccaag gtgagcaagc ccctgaccat caagaacagc
      301 accaagaccc gcagcccctt tgtggcgctg gcggaccgga agtaaggagg agcgtcaatg
      361 aagcgcattt ttgagaagct ggcctttgca aacgtgtttg ccccccagtc tgtggaggca
      421 tccacggaga agaccacggg atatgtggac gcctccggga cggaggaggt ggcctttctg
      481 atctctgccg ccgccctggg aaaggggaag agcctgacgg tgacgctgat ggcggctgat
      541 gacgcctccg ggagcggcgc caaggcggtg ggggaggccg cggtatttac ggacccggtg
      601 gggacggagc cccaggtggt ggcggtgagc tatcaggtgt ctccgctcaa cggccggtat
      661 gtggcggtca agctccagca cgacgccgcg gcggcggtgg tgtgcggggt gctggccgca
      721 gcggatggga tgtatctgcc cccggccaat ggcatgaccc tggtggtatg agatggccat
      781 cccggaggag ctgaagaccg cggtgttcgc ctactgccgg gaggtggagg acgaggagac
      841 cctgccggcc atgacggtgg cctgggatgc ggcggtgggg tatctggagg gggcgggggt
      901 gacccgccct gccccggaga gcacccgcca cggcctctgg ctgggggtgg tgctgcccct
      961 taccctggat gcctatgacc agagggaggc ccagaccgag gggcagaaca agctccagga
     1021 caacccggtg ctgcggcgga aactgaccca gctgaaattg acagaaggga tttaaacccg
     1081 cccacccgtc ctttttgaag gtgtggggct ttgcccccac gcccccggca gccctgcggg
     1141 cggctttgcc gccctggggc aggacggggg agagatacgc ccccgggcgg cggagccgcc
     1201 caccagacca tgacccaggc gggcggaatg gcctgtaggg gcgcatagta tgcgcccgtg
     1261 cagccggcgg cagagggctt gtaggggtgc cgggcggatg atatccgccc ctacgggtcc
     1321 cactatgacc ccaccgagag gcggggacca aaccggggcc cagcgcagcg ggtccggttt
     1381 ggagaggcgg agtaccggaa tgagtgagct ttcgccgaaa ggcggaagcg aaggatatgg
     1441 aggctacgac gacgatggta tcggccagcg gcctgcgggc caggatcacg atttaccgca
     1501 ggaaagagac ggaaaatgag ctggaggaga agaccttcac ctatcagccg gagcggacgg
     1561 tgtgggcgga gatcgtgccc acttccggcc gcacggctcc cctggaggga gccgtggaac
     1621 gggcggagat cacccaccgg gtgcggctgc ggatctctgc ctgccccgtc atcgagcggg
     1681 ggatgtattt taccttccgc agacagcggt atgatgtgct gtacggctac tccatttaca
     1741 accggcgcgg gtggctggag ctgtattgca gattggtggt ggaggacagt gtcccaggga
     1801 tttgatctct cggagctcca gaaatatgca aaggaccttg ctaccgtggg tcaggagtac
     1861 gagaaggcgg agcggagatt tctccagaag gaggggtcta agctgcgcag caagacaaag
     1921 gcacaggccc ggaagctggg caagaagacc ggaaactacc tgaagtccat caagcggggc
     1981 aaggtgtaca agtaccgggg ggacaaggct gtccgggtgt actcctacgc cccccacgcc
     2041 cacctgatcg aggaggggca ccggatggtc acccacgacg gccgggaggt tggctttgtg
     2101 aaaggtcacc atgtgttcga ggtggcggca aagggctttg aatcggagtt ctacaccgat
     2161 ctggacgact ttctcagcgg agaggtggtg gagaagctgt gacgctgaaa gaactgaaca
     2221 aggcggtctg cggtctggtg aagcgggcgg cggaggcctc cggcctgggg gcggagctga
     2281 tggcggagga tctgagcgct cccatccagc ggccctcgct caaagtggag ctggaggagg
     2341 acagagatgc ccgggccatc cggcaccggg tggagcaggc catcaccttc cgggtctatt
     2401 tcttcgcggc ggaccagtac cggcccaagc tggacaacct ggccatgcgc caggccctgg
     2461 cggaggcatt tcgggacggg gtccccgtgg gggaggagac ggtgcccatc gacgaggggc
     2521 tgtcctttac agtgaccgac ggggtgctgg tggcatctat ggacctgacc ctggaccagc
     2581 gcatcattcc tgctgaggaa gaggatgcgg agctcatgga ggagctgaac gtgaatttgg
     2641 aggtgcattg aaatggcggt gacactgcca aaaatcgaag tgacatttaa gcagctggcg
     2701 gccagctttg tccggcgctc agagcggggg atcgcggtgc tgatcctgcg ggacgccaca
     2761 gaggggagcg gcgagagctt cttccggttt ggggttgcca cccaggtccc ggagaatgaa
     2821 tttaccgcgg ccaaccagca gtatatcaag gacgccctct cttttggccc cctgcgggtg
     2881 tcggtggtca aggtgggggc ggaggggacg ctggcccagg ctctggccat tctggtgcag
     2941 aaggagcaga ccgggtggat caccgtgtgc gacggcacat ccagcgactg gaccgacctg
     3001 gtgagctgga tcaaggcccg ggagaaggaa gagaagagct ggaaggcggt gtgctacgat
     3061 accacatccc cagactgcat gcacgtggtg aactttgtca acaagacagt cacctttgcc
     3121 gacgaccggg gagcggtggg gggcggcaag tacaccccca gcctggcggg cctgctggcg
     3181 gcctgcaatg tgctgcgggg ggctaccggc tatgtgtgta gcaacctgac caccgtagag
     3241 gagccggaag acgcagagac ggccgtgggc tccggcaagt tcgtcctggt caacgaggat
     3301 gatgaggtcc aggtgggggt ggacgtgaac agcatgacca ctgtcaacgg aagcacccag
     3361 accgaggaca tgaagtacat cgagacggta gaggccatgg acctgatacg ggacgacatc
     3421 accgccacct tccgcaagga ctaccggggg aaatacaaaa acaccaccgc caaccagatg
     3481 ctgttcctgg ccgtggtcaa cggctatttc cgggacctgg gggacgagaa cgtcctggac
     3541 cccacctatg acaacaccgc cgcggtggat gtgaccagcc agcgcaacgc ctggatcgac
     3601 tcgggcaagg cggaggccgt ggactgggac gacgggaagg tcatcgccac cccgtacaag
     3661 cggaaggtgt tcctgggcgg cgatgtgaag atcctgggct ccatgacgga cctcaaattt
     3721 gtggtcacgc tgatgtaagg agggggatag gcaatggtga aatatcagga ggtcctgcac
     3781 ggctccacca gcgagctgta tatcaacggg gagcgggagg agctggtcac caagatcgaa
     3841 atcaaaatga caggggactt tgaggacagc tccttctgcg gtgagtacgg caccttcccc
     3901 atctacaacg ggtatgccat cgagggaact atctctggaa agaagcgcaa cagcaccctg
     3961 gagaacgcca tcgtggaggg ctaccgcacc ggtgagatgc cggatatcac cgtcatctcc
     4021 gccctgggga gggcggggag ctcagcacgg gaacgctggt cggtgagcgg cgtggtgttc
     4081 acggaggcgg ccctggccaa catcgaggcc aagaaggcag tggagcggga gttgcccttt
     4141 aaggcggtcc tggcaaagaa tttggaggcg atcgagtgat ggcgagaaaa tttagctttg
     4201 acgagctgct ggctaaacgg gagcagcggg aggcggacaa gctgaaggtg gggctgctgg
     4261 agctccctgg caccgggaag ggcctggagg cccggatgcc cgggcagaag acggtgctgg
     4321 acctgtacgg ggagctggta gcggcgcagg acgccaggga gtccctccag tgcgggaatc
     4381 acgcccttta cgcctgctgc ccccagctct gggacagcag gctccaggag gagctgggct
     4441 gcaaggagga ccccatgcag gtgctggaca agctgttttc cctcgcagag caggaccagc
     4501 tgggcgggca ggcgctgcgc ttcctgggcc tgctcccgga accgccccag aagacggacg
     4561 ggaacgacgg ggaagagaag gaagaggccc agggagcggc ccccggtatt gagacggtaa
     4621 aaaactgatc gcccgcgacc ctctcctggg gctgttcgcc ttctacgcgc cccggggctg
     4681 gaccctggag gcgctgagcc ggaccagtcc ggcggagcgg gccttcctgg agggggcgcg
     4741 g
//
LOCUS       DBMDSJ010000088         4697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_4978_length_4697_cov_1.895344, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000088 DBMDSJ010000000
VERSION     DBMDSJ010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4697
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4978_length_4697_cov_1.895344"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="ACZPAQ_03225"
     CDS             complement(<1..232)
                     /locus_tag="ACZPAQ_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HIR0903024.1"
                     /translation="MARNDEKKSIRCSFCGKHQDQVRRLIAGPGAYICNECVQLCMGI
                     LSDDVDLPEEGYAGEIPDEIPTPREIHQVLDQY"
     gene            complement(308..889)
                     /gene="clpP"
                     /locus_tag="ACZPAQ_03230"
     CDS             complement(308..889)
                     /gene="clpP"
                     /locus_tag="ACZPAQ_03230"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR0903025.1"
                     /translation="MSLVPYVVEQTNRGERSYDIFSRLLNDRIIMLSEEVNDTTASLV
                     VAQLLYLEAQDPDKEIQFYINSPGGSVTAGMAIYDTMQYIKCDVSTICIGLAASMGAF
                     LLSSGTKGKRLALPNAEIMIHQPSAGTQGQITDMAIHLKRLEVVKKRMNRILSENTGK
                     AIEVVTADCERDNFMTAQEAVEYGLIDKVIEHR"
     gene            complement(978..2408)
                     /gene="tig"
                     /locus_tag="ACZPAQ_03235"
     CDS             complement(978..2408)
                     /gene="tig"
                     /locus_tag="ACZPAQ_03235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR0903026.1"
                     /translation="MKVTNVEKKEKSTVELTIQVEAQEFEDAVQKAYLKNRAHISVPG
                     FRKGKAPRKIIEGMYGSGVFYEDAINIVYPSAYEEAVKEQGLDDVGYPQTEVVEVGKE
                     GFTFKALVSVRPEVKLGQYKGLTAPKAEPAVTDEDIENELKPYITRATRLVSVDREAQ
                     NGDTAVIDFEGFDNGVAFEGGKGENYSLELGSGSFVPGFEEQIVGLKAGDEKELNITF
                     PEDYVPDLAGKAVIFKVKVKEVKEHQAPVVDDEFAKDVSEFETLEEFKKSLGDKLLDS
                     KTKSAQQEYENAVIEQLVGNMEADIPDAMVEVQLDKLMEDFSMRLSNQGMKMEDYLAM
                     MGATPESMRASARPTAQRQVEVDLALTAVADAESITISDEELEEELKKIAGEYKLELD
                     EVKRAIPVEDMKRDLRLRKASELVMAEAKVGEAPKKEEAEEKPKKTTRKKKAESAEGE
                     EAAAEEKPKKTTRKKKAEEAEEPKAE"
     gene            complement(2530..3216)
                     /locus_tag="ACZPAQ_03240"
     CDS             complement(2530..3216)
                     /locus_tag="ACZPAQ_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR0903027.1"
                     /translation="MALYAIGDTHLSLSSDKPMDVFGGGWTGYVEKLREGLSVLEPGD
                     TVVLCGDLSWGMSLEEARRDFAFLSSFPGRKLLLKGNHDYWWTTAAKMDRFFQENGFS
                     SFQLLHNNSHLYGDIALCGTRGWFYEEERGGHSAKIFNRELIRLEASLKAAGEREKYC
                     FLHYPPLYQGYRCQEILDLLHRYGVTRCFYGHLHGGSHRLAAVGEQDGIQFRLVSADY
                     LRFQPEKICD"
     gene            complement(3220..4695)
                     /gene="thrC"
                     /locus_tag="ACZPAQ_03245"
     CDS             complement(3220..4695)
                     /gene="thrC"
                     /locus_tag="ACZPAQ_03245"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR0903028.1"
                     /translation="MSTRDKNTTYLASQAIAQGLAPDGGLMTPAYLPKLPGKSLSDLK
                     EMSYQQRAVYIMKLFLDEFSVKELTEFAAAAYGPEKFDTPAVAPVRALDDGTFCLELW
                     HGPTCAFKDMALQMLPHLLTASLVKTEEEKTVCILVATSGDTGKGALEGFRDVDRTRI
                     LVFYPKDGVSAIQELQMVTQEGKNVGVCSVFGNFDDAQTGVKKLFSDEALRQELARRG
                     YFLSSANSINWGRVLPQIVYYISAYCDLLRDEKIQPDQAVNVCVPTGNFGNILAAYYA
                     REMGVPIDRLICASNSNNVLTDFIRTGRYDRNRAFHNTISPSMDILISSNLERLLLAL
                     TQDDEQVKDYMAQLSATGTYQVSDRVREKLQKRFAGYFCDDAETKAVIGRIYQKYDYL
                     IDPHTAVAFDALEQYRSETGDQTPCIVVSTASPFKFCDSVLSALGEEEKQNGLELLDE
                     LSEKTGMPAPKPLASLRDKEIRFTQTVEKDHMVDAVLGMLE"
BASE COUNT          806 a         1482 c         1367 g         1042 t
ORIGIN      
        1 tatactggtc cagaacctga tggatctccc ggggcgtagg gatctcgtca gggatctctc
       61 cagcgtagcc ctcctctggc aggtccacat cgtcgctgag gatgcccatg cacagctgga
      121 cacactcgtt gcagatatag gcgcccgggc cggcgatcag ccgccggacc tggtcctgat
      181 gcttgccgca gaaggagcag cggatggact ttttttcgtc gtttcttgcc ataatgcacc
      241 tcattgttgt gcgttcagat aggaatcgga gtgggaacag aagcctgttc ccactcccct
      301 gtttccttta acggtgttcg atgaccttgt cgatcaggcc gtactccacg gcctcctggg
      361 cggtcatgaa gttgtcccgc tcacagtcgg cggtgaccac ctcgatggcc ttaccggtgt
      421 tctccgacag gatgcggttc atccgcttct tgaccacttc cagccgcttc aggtggatcg
      481 ccatgtcggt gatctgcccc tgggtcccgg cagaaggctg gtggatcatg atctcggcgt
      541 tgggcagggc cagccgcttg cccttggtcc cggaggacag caggaacgcc cccatggagg
      601 cggccagtcc gatgcagatg gtggacacgt cgcacttgat atactgcatg gtgtcataga
      661 tggccatgcc ggctgtcacc gatccgccgg ggctgttgat gtagaactgg atctccttgt
      721 ccgggtcctg ggcctccaga tacagcagct gggccaccac cagagaggcg gtggtgtcgt
      781 tgacctcctc ggagagcatg atgatccggt cattgagcag tcgggagaag atgtcgtagg
      841 aacgctcccc ccggttggtc tgctcgacta cataaggaac taaactcata gaatgatctc
      901 ctttgacttg ggcagtgata ccaatagtat gcccgcccgc ccggagcgca tacctccgga
      961 cacaaaaacc ttggaaatta ctccgccttg ggctcctcag cctcctcggc cttcttcttg
     1021 cgggtggtct tcttgggctt ctcctcggcg gcggcctcct cgccctcggc gctctcagcc
     1081 ttcttcttgc gggtagtctt cttgggcttc tcctcggcct cttccttctt gggggcctcc
     1141 cccaccttgg cctcggccat caccagctca gaggcctttc tcaggcgcag atcccgcttc
     1201 atatcctcca cggggatggc tctcttcacc tcatccagct ccagcttgta ctccccggcg
     1261 atcttcttca gctcttcctc cagctcctcg tcggagatgg tgatgctctc ggcgtcggcc
     1321 acagcggtga gagccaggtc cacctccacc tgacgctgag cggtggggcg ggcagaggcg
     1381 cgcatggact cgggggtggc gcccatcatc gccaggtagt cctccatctt catgccctgg
     1441 ttggacaggc gcatggagaa gtcctccatc agcttgtcca gctggacctc caccatggca
     1501 tcggggatgt cagcctccat gttgcccacc agctgctcga taacagcgtt ctcatactcc
     1561 tgctgggcgg acttggtttt gctgtccagc agcttgtcgc ccaggctctt cttgaactcc
     1621 tccagcgtct cgaactcaga cacgtccttg gcaaactcgt cgtccaccac gggggcctgg
     1681 tgctccttga cctccttgac cttcaccttg aagatcacgg cctttccggc caggtcaggc
     1741 acatagtcct cgggaaaggt gatgttcagc tccttctcgt cgccggcttt caatcccacg
     1801 atctgctcct cgaagccggg gacaaaggaa ccggagccca gctccaggct gtaattttct
     1861 cccttaccgc cttcgaaggc cacgccgttg tcaaagccct caaagtcgat gacggcggtg
     1921 tccccgttct gggcttcccg gtccacgctg accaggcggg tggcgcgggt gatgtagggc
     1981 ttgagctcgt tctcaatgtc ctcgtcagtg acggccggct cagccttggg ggcggtgagg
     2041 cccttgtact ggcccagctt cacctcggga cggacggaga ccagggcctt gaaagtaaag
     2101 ccctccttgc cgacctccac tacctctgtc tgggggtaac ccacatcgtc caggccctgc
     2161 tccttcacag cttcctcata ggcgctgggg tagacgatat tgatggcatc ctcataaaac
     2221 acgccggagc cgtacatccc ctcaatgatc ttgcgggggg ccttgccctt ccggaagccg
     2281 ggcacgctga tatgggcgcg gttcttcaga taagccttct gaacggcgtc ttcaaactcc
     2341 tgagcttcca cctggatggt cagctccacg gtgctctttt ctttcttttc cacgttggtg
     2401 accttcatgt ttccttttcc tcctgtaggc gcctggtccc ctcttgggaa cagcccgcct
     2461 ttttactttc gcctggatat tctaacagat gtggctctgt ttgaccagag atatttttga
     2521 tttttccgct caatcgcaga ttttttctgg ttgaaaccgc agataatccg cggagaccag
     2581 ccggaactgg atgccgtcct gctctcccac cgctgccagg cggtggctcc ccccgtggag
     2641 gtgcccgtaa aagcagcggg tcacgccgta ccgatggagc agatccagga tctcctggca
     2701 ccggtacccc tgatacaggg gcggatagtg caggaagcag tatttctccc gctcccccgc
     2761 cgccttcagt gatgcctcca gccggatcag ctcccggttg aagatcttgg cactgtgccc
     2821 gcctcgttcc tcctcgtaga accagccccg ggtgccgcac agggcgatgt ctccatagag
     2881 atggctgttg ttgtggagca gttgaaagga agaaaaccca ttttcctgga aaaagcggtc
     2941 cattttggct gcggtggtcc accagtagtc atggttcccc ttcaaaagca gcttccgccc
     3001 cgggaaggaa ctgaggaaag caaagtcccg cctcgcctcc tccaggctca tgccccagga
     3061 cagatccccg cagagcacca cggtgtcccc gggctccagc acggacagtc cctcccgcag
     3121 cttctccaca tatcccgtcc atccgccgcc gaagacatcc atgggcttgt cggagctgag
     3181 ggacaggtgg gtatctccaa tggcgtacag ggccatcgct tactccagca tccccagcac
     3241 cgcgtccacc atgtggtcct tttccacggt ctgtgtgaag cggatctcct tgtcccggag
     3301 ggatgccagc ggcttggggg cgggcatccc ggtcttttcg ctcagctcgt ccagcagctc
     3361 cagcccgttc tgcttctcct cctcgcccag ggcgctgagg acgctgtcgc agaacttgaa
     3421 gggactggcg gtggagacca cgatgcaggg ggtctggtcc cccgtctcgc tccggtactg
     3481 ctccagggcg tcgaaggcca cggcggtgtg ggggtcgatg aggtagtcgt acttctgata
     3541 gatgcggccg atcacggcct tggtctccgc gtcgtcgcag aaatagccag cgaaccgctt
     3601 ctgcagcttc tccctcaccc ggtcgctgac ctgataggtc ccggtggcgg acagctgggc
     3661 catgtagtcc ttcacctgct cgtcatcctg ggtcagcgcc agcaggagcc gctccaggtt
     3721 gctggagatc aggatgtcca tggagggaga gatggtgttg tggaaggcgc ggttgcggtc
     3781 atagcggccg gtgcggatga agtcagtgag cacattgttg ctgttggagg cgcagatcag
     3841 cctgtcgatc gggaccccca tctccctggc atagtaggcg gccaggatgt tgccgaagtt
     3901 tcctgtgggg acgcagacgt tcaccgcctg gtctggctgg atcttctcgt cccgcagcag
     3961 gtcgcagtag gcggagatat aataaacgat ctggggcagc acccggcccc agttgatgga
     4021 gttggcggag gagaggaagt acccccggcg ggccagctcc tggcggagcg cctcgtcgga
     4081 aaagagcttc ttgactccgg tctgggcatc gtcaaagttg ccgaagaccg agcagacgcc
     4141 cacattcttc ccctcctggg tgaccatctg aagctcctgg atggcggaga caccgtcctt
     4201 ggggtagaag accaggatgc gggtgcggtc cacatcccgg aagccctcca gcgccccctt
     4261 cccagtgtcc ccggaggtag ccaccaggat gcacaccgtc ttctcctcct ccgtcttcac
     4321 cagagaggct gtaagcagat ggggcagcat ctgaagggcc atgtccttga aagcacaggt
     4381 ggggccgtgc cacagctcca agcagaaggt tccgtcatcc agggcgcgga ccggggccac
     4441 ggcgggagtg tcgaattttt ccggcccgta ggccgccgca gcgaactccg tcagctcctt
     4501 gacggagaac tcgtccagaa acagcttcat gatatagacc gcccgctgct gataggacat
     4561 ctccttcagg tcagacaggg acttgcccgg cagcttgggc agatacgccg gggtcatcag
     4621 gccgccgtcc ggggccagcc cttgggcgat ggcctgagat gccagatatg tggtgttctt
     4681 atctcgtgtg ctgatgt
//
LOCUS       DBMDSJ010000089         4636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5036_length_4636_cov_1.678280, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000089 DBMDSJ010000000
VERSION     DBMDSJ010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5036_length_4636_cov_1.678280"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="yfmF"
                     /locus_tag="ACZPAQ_03250"
     CDS             <1..1179
                     /gene="yfmF"
                     /locus_tag="ACZPAQ_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmF"
                     /protein_id="HIR0903029.1"
                     /translation="LERERAAANALLFPVLRRGTREHPDMESLSAALDELYGGAIEPI
                     VRKKGETQCLGFVASFLDDAFAPGGEPILEPAAGLLGELLLRPRLQDGIFAEEYVSGE
                     RANLVDRIRGQINDKRSYATLRLGQLMCGEEAFGVDKLGDVEHAAAVTPGSLWERYQQ
                     LLSTAQIELYYCGSAEEERVARALKEALSALPVNEGRICPDCEVRLHAGPEPSMVEES
                     MDVTQGKLAMGFRTGGVTCWEEEYPAMAMCNAVFGGTTLSKLFMNVREKLSLCYYASS
                     TLEKMKGLILVSSGIEFDKFQQARDEILHQLEEIRQGNMEDWELEGTRRTMIGAHLAT
                     LDVQDRQEDFWLGQTAAGLETGIEELVAQLEQVTREQVAQAAQKLELDTIYFLKGTEG
                     "
     gene            1183..2469
                     /gene="yfmH"
                     /locus_tag="ACZPAQ_03255"
     CDS             1183..2469
                     /gene="yfmH"
                     /locus_tag="ACZPAQ_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIR0903030.1"
                     /translation="MEKLEFPRVGEQMYHQVLDNGLHIFVVPKPGFQKSYAFFATNYG
                     GMDMKFKLDGQDMDTPAGVAHYLEHKMFDTKEGNALQELAANGASPNAFTSNAITGYY
                     FESTEKFEENLRILLSFVSIPYFTKESVDKEQGIIGQEIGMIEDDPDWVVFMDLMQAL
                     YEKHPVRVSVAGSAESISHITAETLYACHKAFYDPSNMVLCVAGDVDPEKICQAAREI
                     LPERPGAPVARGDYGAQEPAEVCQPMIQREMEVSVPLFQLGFKGEAAPEGEGALRQQL
                     LGELACEALLGNSGPLYARLYREGLVNRNFSYGYESYPGCAFLCAGGESKDPEGVRAA
                     VAAEAERIAREGIDPELWERLKKSSYGNRVRALNSFEHICISQAQSFFQGCDFLRFPE
                     VYDSLTREDAQAMIARWVTPERTALSVVRPREEAVE"
     gene            2466..3437
                     /gene="lgt"
                     /locus_tag="ACZPAQ_03260"
     CDS             2466..3437
                     /gene="lgt"
                     /locus_tag="ACZPAQ_03260"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR0903031.1"
                     /translation="MTNIVTFPGLGLEFHLDRVAFSLFGHPVYWYGVIIGCGFLLAVL
                     LCCRWAPRFGVNSDQIMDLLIFAVPLSVVGLRVYYVLFYLDLYREADGSLNWGAIFRI
                     TDGGLAIYGGIIAAALTLIVFCRIRKISFFSFADLGVHGLLIGQCIGRWGNFMNVEAY
                     GGVTGLPWRMCSESIASSLLSQGLVDTDGYQAILDGTLGVHPTFFYESLWNFVGIFLI
                     YWIGKHWKKFDGQVFWSYVLWYGLGRFWIEGLRTDSLYFFGLELFGAPIRTSQVLAIV
                     SAAVAAVVLIYELKFRRHDPSELYVNRLAAHRADAGEGPGGPEKKED"
     gene            3441..4283
                     /gene="folD"
                     /locus_tag="ACZPAQ_03265"
     CDS             3441..4283
                     /gene="folD"
                     /locus_tag="ACZPAQ_03265"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR0903032.1"
                     /translation="MSAVIMDGKALAKKVKEEVRAAAARMERRPGLAVILVGDDPASQ
                     VYADGKKRDCAQCGIYSEEYILPAETGQEELMLLIETLNGRSDIDGILIQLPLPAHLN
                     ERAVLSVVRPDKDVDCFHPFNVGELMIGEKGFYPCTPASVMRLLAEYGVELSGKSCVI
                     VGRSNIVGKPLAMLMLRENATVTICHTKTEYLKKECSRADILISAAGQAGLITGDMVK
                     EGAVVVDVAVNRRADGTLCGDVVFEEAAGRASYITPVPGGVGPLTRAMLMKNTLYAAV
                     IHGK"
     gene            4428..>4636
                     /locus_tag="ACZPAQ_03270"
     CDS             4428..>4636
                     /locus_tag="ACZPAQ_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903033.1"
                     /translation="MRTKVRTRLLKFTMAMEIVMAITILGAILASIALLICGSVEKFC
                     CQWESFSIDAFMANAFLIVMGIEFV"
BASE COUNT          848 a         1328 c         1606 g          854 t
ORIGIN      
        1 ctggagcggg agcgggcggc ggccaatgcc ctgctcttcc cggtgctgcg ccgggggacc
       61 cgggagcacc cggatatgga gtccctctcc gccgccctgg acgagctgta cggaggcgcc
      121 atcgagccca tcgtgcggaa aaagggggag acccagtgcc tgggctttgt ggccagcttt
      181 ctggatgatg cctttgcgcc cgggggcgag cccatcctgg agcccgcggc ggggctgctg
      241 ggggagctgc tgctccgccc ccgccttcag gacggcattt ttgcggagga atatgtgtcc
      301 ggggaacggg ccaatctggt ggaccgcatc cggggccaga tcaacgacaa gcgctcctac
      361 gccaccctgc gcctgggaca gctgatgtgc ggggaggagg ccttcggggt ggacaagctg
      421 ggggatgtgg agcatgccgc ggccgtcacg ccgggctctc tgtgggagcg gtatcagcag
      481 ctgctctcca ccgcccagat cgagctgtac tactgcggtt ccgcggagga ggagcgggtg
      541 gcccgcgccc tgaaggaggc gctgtccgcc ctccccgtga atgaggggcg gatctgcccg
      601 gactgcgagg tccggctcca cgccggaccg gagcccagca tggtggagga gtccatggac
      661 gtgacccagg gcaagctggc catgggcttc cgcaccggcg gcgtcacctg ctgggaggag
      721 gaataccccg ccatggccat gtgcaacgcc gtattcggcg gcactaccct gtccaagctc
      781 tttatgaacg tgcgggagaa gctgtctctg tgctactacg ccagctccac cctggagaag
      841 atgaaggggc tgatcctggt ctcctccggg atcgaatttg acaagttcca gcaggccagg
      901 gacgagatcc tccatcagct ggaggagatc cggcagggga acatggagga ctgggagctg
      961 gaggggaccc gccgcaccat gatcggcgcc cacctggcca ccctggatgt ccaggaccgg
     1021 caggaggact tctggctggg ccagacggcc gccggtctgg agaccggcat cgaggagctg
     1081 gtggcccagc tggagcaggt cacccgggag caggtggccc aggcggctca gaagctggaa
     1141 ctggacacca tttacttcct gaagggaacg gagggctgaa ccatggagaa actggaattt
     1201 ccccgggtgg gggagcagat gtaccaccag gtgctggaca acggactgca tatctttgtg
     1261 gtgcccaagc ccggctttca gaagagctac gccttttttg ccaccaatta cggaggcatg
     1321 gatatgaagt tcaagctgga tggccaggat atggacactc cggcgggggt ggcccactat
     1381 ctggagcaca agatgttcga caccaaggag ggcaacgccc tccaggagct ggcggccaac
     1441 ggcgcctccc ccaatgcctt caccagcaac gccatcacag gctactattt cgagagcaca
     1501 gagaaatttg aggagaacct gcgcatcctg ctctccttcg tctccattcc ctatttcacc
     1561 aaggagagcg tggacaagga gcaggggatc atcggtcagg agatcggcat gatcgaggac
     1621 gacccggact gggtggtctt tatggatctg atgcaggccc tctacgagaa gcacccggtg
     1681 cgggtgtcgg tggcgggctc ggcggagtcc atctcccaca tcaccgctga gaccctctac
     1741 gcctgccaca aggcattcta cgacccctcc aatatggtgc tgtgcgtggc aggggacgtg
     1801 gacccggaga agatctgcca ggcggcccgg gagatcctgc cggagcggcc tggagcaccg
     1861 gttgcccggg gggactacgg cgcccaggag cccgcggagg tgtgccagcc catgatccag
     1921 cgggagatgg aggtctcggt ccccctgttc cagctgggat tcaaggggga ggcggccccg
     1981 gagggagagg gggccctgcg ccagcagctg ctgggcgagc tggcctgtga ggcacttctg
     2041 ggcaactccg gcccgctgta tgccagactc taccgggagg ggctggtcaa ccggaatttc
     2101 tcctacgggt atgagagcta ccccggctgc gctttcctgt gtgccggggg ggagagcaag
     2161 gacccggagg gagtgcgggc ggcggtggcc gccgaggcgg agcggatcgc acgggagggg
     2221 atcgaccccg agctgtggga gcggctgaaa aaatcctcct acggaaaccg ggtgcgggcg
     2281 ctgaactcct ttgagcacat ctgcatcagt caggcccagt cgtttttcca gggctgcgat
     2341 ttcctgcgct tcccggaggt gtacgacagc ctgaccaggg aggacgccca ggccatgatc
     2401 gcccgctggg tcacgccgga gcgcaccgcc ctgtccgtgg tccggcccag agaggaagcg
     2461 gtggaatgac caacatcgtg acatttcccg gactggggct ggagttccac cttgaccggg
     2521 tggccttctc gttgtttggc cacccggtgt actggtacgg ggtcatcatc ggctgcggat
     2581 ttctgctggc cgtcctgctg tgctgccggt gggcgcccag attcggcgtg aacagcgacc
     2641 agatcatgga cctgctgatc ttcgcggtgc ccctctccgt ggtggggctg cgggtctatt
     2701 acgtactctt ctatctggac ctgtaccggg aggcggacgg ttccctgaac tggggggcca
     2761 tcttccgcat tacagacggc ggcctggcca tctacggcgg catcattgcc gccgccctga
     2821 ccttgatcgt gttctgccgt atccggaaga tcagcttctt ctcctttgca gacctggggg
     2881 tccacggcct tctgatcggc cagtgcatcg gccggtgggg aaactttatg aatgtggagg
     2941 cctacggcgg cgtcaccgga cttccctggc ggatgtgttc ggagagcatc gccagctccc
     3001 tgctgtccca gggactggtg gacacggacg gctatcaggc catcctggac ggcaccctgg
     3061 gggtgcaccc tacgtttttc tacgagtccc tgtggaattt tgtgggcatc tttctgatct
     3121 actggatcgg aaagcactgg aagaaatttg atggccaggt gttctggagc tatgtgctgt
     3181 ggtacggcct gggccggttc tggatcgagg gcctgcggac ggacagcctg tatttcttcg
     3241 gactggagct gttcggcgcc cccatccgca cctcccaggt gctggccatc gtctctgccg
     3301 ccgtggcggc tgttgtattg atctatgagc tgaagttccg tcggcacgat ccgtctgagc
     3361 tttatgtgaa ccggctggcc gcccacaggg cggacgctgg agagggcccg ggcggaccgg
     3421 agaaaaagga ggactgattt gtgtcggcgg tcataatgga cggaaaggca ctggcaaaaa
     3481 aggtgaagga ggaggtccgg gcggcggcgg cgcggatgga gcgccgcccc ggcctggccg
     3541 tgatcctggt aggggacgac cccgccagcc aggtctacgc ggatggaaag aagcgggact
     3601 gtgcccagtg cgggatatac agtgaggaat acatcctgcc ggcggagaca ggacaggagg
     3661 agctgatgct gctcattgaa acgctgaacg gacgcagcga catcgacggc atcctgatcc
     3721 agctgcccct gccggcccat ttgaatgagc gggcggtcct gtctgtggtc aggcccgaca
     3781 aggatgtgga ctgcttccac cccttcaacg tgggggagct gatgatcggg gagaagggct
     3841 tttatccctg caccccggcc agcgtgatgc gtctgctcgc ggaatacggc gtggagctgt
     3901 cggggaaaag ctgcgtgatc gtggggcgct ccaatatcgt gggcaagccg ctggctatgc
     3961 tgatgctgcg ggagaacgcc acggtcacca tctgccacac caagacggag tacctgaaaa
     4021 aggagtgcag ccgggcggac atcctgatct ccgccgccgg acaggccggc ctcatcactg
     4081 gagacatggt gaaggagggg gccgtggtgg tggatgtggc agtgaaccgc cgggcggacg
     4141 ggaccctgtg cggcgacgtg gtatttgagg aggcggccgg acgggcgtcg tacatcaccc
     4201 cggttccggg gggcgttggg ccgctgaccc gggccatgct gatgaagaac acgctgtacg
     4261 cagcggtgat ccacggaaaa tagatccgga agcgggcgga gagggggagt catctcccac
     4321 ctctccgccc gtccttttta ctgtaaaaaa gatggggaaa tgattgacgg accaggtggc
     4381 tatcccatat actggtctta cataaaatcg tgctgcggga gggccgtatg agaacaaaag
     4441 taaggacaag actgctgaag tttaccatgg cgatggagat cgtgatggcg atcaccatcc
     4501 tgggggcgat cctggccagc attgcactgc tgatctgcgg ctcggtggag aagttctgct
     4561 gccagtggga gtccttctcc attgacgcat ttatggcaaa tgcgttcctc atcgtcatgg
     4621 ggatcgagtt tgtaaa
//
LOCUS       DBMDSJ010000090         4590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5087_length_4590_cov_1.699042, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000090 DBMDSJ010000000
VERSION     DBMDSJ010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5087_length_4590_cov_1.699042"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="ACZPAQ_03275"
     CDS             <1..560
                     /locus_tag="ACZPAQ_03275"
                     /inference="COORDINATES: protein motif:HMM:NF021468.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HIR0903034.1"
                     /translation="PGAFPQKASVCPLSGGDEVNFYQLLPLYQEEMDFKLSHSAGELL
                     DLFPEEDLETVDVDRPSVLSDRPQKEFAIPQRELRHLYDGEGPQGCFATDRILVDGCR
                     VGYCYREEPEEGDENWDSGWRFTAGDESDSYMDDPGRSGIHHLNTLCNYDPDIIPLLD
                     SEPGTAWCRDQSGVFRPELYQPDEE"
     gene            585..1205
                     /locus_tag="ACZPAQ_03280"
     CDS             585..1205
                     /locus_tag="ACZPAQ_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR0903035.1"
                     /translation="MYTCASCTLRSCQKGGGDLPRNCPMRDPEFFDRLLERYWSEENH
                     DFFVTSAAIESAGYCRWPRLKEIVEFSRSMHYQKLGLAFCAALKKEAAVVERVLRNNG
                     FQVVSVICKTGGLDKSRAGVPEECKLQPGQFEAMCNPIAQAELLNSQDTQFNICLGLC
                     VGHDSLFYQYSKALVTTLVVKDRVLAHNPVGAIHYADTYFKDLLKG"
     gene            1437..2105
                     /locus_tag="ACZPAQ_03285"
     CDS             1437..2105
                     /locus_tag="ACZPAQ_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR0903036.1"
                     /translation="MPVSLPPHQWDLLLRCPLFQACPEALVRDVLARSGCSVEDFSSG
                     AEIYRPDRFRRCLGLLLSGRVLVTKGSLTVSTLEPGDLFGAAALYNDAPDYATTLTAQ
                     GICRCLLLDQELLDRLLGEEALLRRNYLTYLTGRIRFLNARLGSLAVGDTEGRVVRYL
                     LSSLSYGAVECSASGLARQLGISRASVYRAFQDLEQAGLIRREGRAILVPDPAALEGV
                     RGSG"
     gene            2198..3262
                     /locus_tag="ACZPAQ_03290"
     CDS             2198..3262
                     /locus_tag="ACZPAQ_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0903037.1"
                     /translation="MKRTKLLPLLLSALLLLSASGCRPAEAPSQSGGAISSVSSSTSS
                     SGIPAPNDEVLPIDLAVLSGPTGVGAAYLMEYYAPEHVPADSPISVSSVVVTENSEAT
                     ALLSNGEADIAAVATNVASNFYHKTDGSIQMLAVNTMGVLYILEKGDSIQSMADLRGK
                     TIYATGQGANPEYILNYLLTQNGLDPAADVTIQWLTAQEVTANMLSAEDGVCMLPVPA
                     ATALLMKDPGVRQALDLSAEWDAVSSTPLPMGCIVARREFVEEHPEAVDAFLDLYGDS
                     ISFMSDEGNRSEAAELVAKYGITANAQIAEAAIPQCNLTLLTGEEMQDTVQAYYEVLY
                     RADPAAIGGSIPYDSLYYLP"
     gene            3237..4001
                     /locus_tag="ACZPAQ_03295"
     CDS             3237..4001
                     /locus_tag="ACZPAQ_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0903038.1"
                     /translation="MTACTICPDRLLRPLLPLAFWLVVWQLAALAVGQSFILPGPAAV
                     LGTLIQLAGVPSFWQTALLSLVRVLAGLLAGTLLGALTAALTCASRWADVLLSPAVRV
                     IRAVPVVSFILFVLLWTRRGLVPVVVSALMVLPVVWGNVGRGIRETDPKLLEQAGALR
                     FSRLKTVRLVYLPSVRPYFLSAVVTSLGLAWKSGVAAEVLCRPAWAVGTQIVQARDYL
                     ETPSLFAWTLVILLLSLTLEKLLSALLNRINRRWSP"
     gene            3998..4588
                     /locus_tag="ACZPAQ_03300"
     CDS             3998..4588
                     /locus_tag="ACZPAQ_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0903039.1"
                     /translation="MICLEEVTVTFGEKRVLDRFSLTLPETGITALSGPSGCGKTTLL
                     RVLAGLEHPISGRITGLSLRSAALLFQEDRLFPWRTVEQHLTDVLPRPRWVEVPRWLR
                     LAELEGEERTYPAHLSGGMGRRLALVRTLALGGSLYLLDEPFAGVDPQRADRILSALG
                     ELAAPVILVSHEPAVLDRADRVIRLDGPPLTLLSAP"
BASE COUNT          718 a         1603 c         1372 g          897 t
ORIGIN      
        1 accccggtgc tttcccccag aaggcgtcgg tctgtcccct ctccggcggc gatgaggtaa
       61 atttctatca gctcctccct ctgtatcagg aggagatgga cttcaagctc tcccacagtg
      121 ccggagagct gctggacctc tttcccgagg aggacctgga gacggtggac gtggaccgcc
      181 ccagcgtcct ctccgaccgc ccccagaagg agttcgccat cccccagcgg gagctccggc
      241 acctctatga cggggagggc ccccagggct gcttcgccac tgaccgcatc ctggtggacg
      301 gctgccgggt gggctactgc tacagggagg agccggagga gggggacgag aattgggata
      361 gcggctggcg cttcaccgcc ggagatgaga gcgacagcta tatggacgac cccggccgca
      421 gcggcatcca tcacctcaac accctgtgca actacgaccc ggacatcatc cctctgctgg
      481 actccgaacc aggcaccgcc tggtgccggg accagagcgg agtgttccgt ccggagctgt
      541 atcagcctga cgaagaatga gagactgtaa ggaggatctg ttgtatgtac acctgcgcca
      601 gctgtaccct ccgctcctgc cagaaggggg gcggggacct gccccggaac tgtcccatgc
      661 gggacccgga gttctttgac cggcttttgg agcgctactg gtcagaggaa aaccacgact
      721 tttttgtcac cagcgccgcc atcgagtcgg ctggctactg ccgctggccc cgcctgaagg
      781 agatcgtgga gttctcccga tccatgcact atcaaaagct gggtctggct ttctgtgcgg
      841 cactgaagaa ggaggccgct gtggtggagc gggtcctgcg gaataacggc ttccaggtgg
      901 tgtccgtcat ctgcaagacc ggcggactgg acaagtcccg ggccggggtg ccggaggagt
      961 gcaagctcca gcccggccag tttgaggcca tgtgcaaccc cattgcccag gccgagctgc
     1021 tcaacagcca ggacacccaa ttcaatattt gtctggggct gtgtgtgggc cacgactccc
     1081 tcttctatca atactccaaa gcactggtca ccactctggt ggtaaaagac cgggtgctgg
     1141 cccacaaccc agtgggcgcc atccactacg cagacaccta tttcaaggat ctgctcaagg
     1201 gctgacccct ggggcggaac gatatggtaa aaacgcctgg ggaggcagac agggtcgtct
     1261 gtctccccag gcgttttacc atacaggtaa ttccctctcc cttccgtgcg cgcccctcct
     1321 catataccgg tcttacatgt tttcatggcg tatgctcttt cccttgacat ttccctccag
     1381 ctgttctaca ataaaaccgt ctcaaatgag acagttttgg aaaggagctg acctccatgc
     1441 ctgtctctct ccctccgcac cagtgggatc tgctcctccg ctgccccctg tttcaggcat
     1501 gtcccgaggc actggtccgg gatgtcctgg cgcggtccgg ctgttcggtg gaggacttct
     1561 cctccggcgc agagatctac cgacccgacc gcttccgccg ttgtctgggg ctgctcctct
     1621 ccggacgggt actggtgacc aagggcagcc tcaccgtcag caccctggag ccaggggatc
     1681 tcttcggcgc cgccgccctc tacaacgacg cccccgacta tgccaccacc ctcaccgccc
     1741 aagggatctg ccgctgcctc ctactggacc aggagctgct ggaccgcctt ctgggggagg
     1801 aggccctgct gcgccggaac tatctgacct atctcaccgg ccgcatccgc ttcctcaacg
     1861 cccggctggg ttccctggct gtgggggata cggagggccg ggtggtccgc tatctgctca
     1921 gcagcctctc ctatggcgca gtggagtgct ccgcctccgg tctggcccgc cagctgggca
     1981 tcagccgcgc ctcggtgtat cgggcctttc aggacttgga acaggccggc ctgatccggc
     2041 gggagggacg ggccatcctc gtccccgatc cggccgctct ggagggtgtc cggggctccg
     2101 ggtaagaaaa tttccccttc ggtgtacaaa tccagtcaac gatccgtctc tgcccagaat
     2161 aggtgtatcc gtccaaatct gagaaaggaa tcctgccatg aaacgaacca agcttctgcc
     2221 cctgctcctg tccgccctgc tcctcctctc tgcctcgggc tgccgtcccg cagaggcacc
     2281 ctcccagtct ggcggcgcca tctcctccgt cagctccagc acctcctctt ccggcatccc
     2341 cgccccgaac gatgaggtcc tccccatcga tctggccgtc ctttccggcc ctaccggtgt
     2401 aggggctgcc tatctaatgg agtactacgc cccggaacac gttcctgctg acagccccat
     2461 ctccgtcagt tccgtcgtgg tgacggaaaa cagcgaggcc accgccctgc tgtccaacgg
     2521 cgaggcagac atcgccgctg tggccaccaa tgtggcctcc aacttctacc acaagaccga
     2581 cggctccatt cagatgctgg cagtgaacac gatgggtgtg ctgtacatcc tggagaaggg
     2641 ggactccatc cagtccatgg ccgacctgcg gggcaagacc atctacgcca ccggccaggg
     2701 ggccaacccg gagtacatcc tcaactatct gctgacccag aacgggctgg accccgccgc
     2761 ggatgtgacc atccagtggc tcaccgccca ggaggtcact gccaacatgc tttccgccga
     2821 ggacggcgtg tgcatgctgc ccgtccccgc cgccactgcc ctgctgatga aggaccccgg
     2881 cgtgcgccaa gccctggacc tgagcgctga gtgggatgcc gtctcctcca cccctctgcc
     2941 catgggctgc atcgtagccc gcagggaatt cgtggaggag catcctgagg ccgtggacgc
     3001 tttcctggac ctgtacggtg actccatctc cttcatgtct gacgagggca accggtcgga
     3061 ggcggcggag ctggtggcca aatacggcat caccgccaac gcccagatcg cggaggccgc
     3121 catcccccag tgcaatctca ccctgctcac cggggaggag atgcaggaca ccgtccaggc
     3181 ctactacgag gtcctgtacc gcgccgaccc cgccgctatc ggcggcagca tcccctatga
     3241 cagcctgtac tatctgccct gaccgtctgc tgcggcccct ccttcccctg gccttctggc
     3301 tggtggtgtg gcagctggcc gccctggctg tgggccagtc cttcatcctt cccggccctg
     3361 ccgcggtcct gggaacgctg atccagctgg cgggggtgcc cagtttctgg cagaccgccc
     3421 tgctctccct ggtccgggtc ctggccggac tgctggccgg cactctgctg ggggcgctga
     3481 ccgccgccct cacctgcgcc agccgatggg cggatgtcct cctttccccc gccgtccggg
     3541 tcatccgggc ggtgccagtg gtctccttca tcctcttcgt cctgctgtgg acccgccgtg
     3601 gtctggtgcc tgtggtggtc tccgccctga tggtgctgcc ggtggtgtgg gggaacgtgg
     3661 gccggggcat ccgggagacc gaccccaagc tgctggaaca ggccggagcg ctccggttct
     3721 cccgcctcaa aaccgtccgg ctggtctatc tcccttcggt gcggccctac tttctcagcg
     3781 cagtggtcac ctctctgggc cttgcctgga agtccggggt ggccgccgag gtgctctgcc
     3841 gccccgcctg ggcggtgggc acgcagatcg tccaggcccg ggactatctg gagaccccct
     3901 ccctgtttgc ctggacgctg gttatcctcc ttctgtccct taccctggaa aaactgctct
     3961 ctgctctgct gaaccggata aacaggaggt ggtcgccatg atctgtttgg aggaggtcac
     4021 cgtcaccttt ggagagaagc gggtgctgga ccgcttctcc ctcaccctgc ctgagacggg
     4081 gatcaccgcc ctgtccggcc cctccggctg cggcaagacc accctgctgc gggttctggc
     4141 cgggctggag caccccatct ccggccggat caccggcctg tccctccgtt ccgctgccct
     4201 gctctttcag gaggaccgcc tcttcccctg gcgtaccgtg gagcagcacc ttaccgacgt
     4261 gctcccccgc ccccggtggg tggaggtccc ccgctggctg cggctggcag agctggaagg
     4321 ggaagagcgt acctatcccg cccatctgtc cggcggcatg ggacggcgtc tggccctggt
     4381 ccgcacgctg gccctgggcg gttccctcta tctgctggac gagccctttg caggggtgga
     4441 ccctcagcgg gcagaccgca tcctatccgc cctgggggag ctggccgccc ctgtcatcct
     4501 ggtcagccat gagcctgccg ttctggaccg ggcggaccgg gtcatccgat tggacgggcc
     4561 gcccctgact ctcctgtccg ccccatagta
//
LOCUS       DBMDSJ010000091         4548 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5145_length_4548_cov_1.498089, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000091 DBMDSJ010000000
VERSION     DBMDSJ010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5145_length_4548_cov_1.498089"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="ACZPAQ_03305"
     CDS             <1..578
                     /locus_tag="ACZPAQ_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HIR0903040.1"
                     /translation="YAFIGPTVPELTHAPRSGGDRQIYISLGTVNNRSRSFYRACLEA
                     LRSWEGVRAVLSLGGGCRPEELGDVPPNVQVLPQVDQLAVLSQSDAFLSHCGMNSVSE
                     SLYLGVPLVLFPQQEEQGMVARRVQELGAGVPLPAPTPAAIHQALSLVLADPAFQHSA
                     QHISEGFRAAGGYRRGADKILEAAMGSAPLR"
     gene            complement(660..1049)
                     /locus_tag="ACZPAQ_03310"
     CDS             complement(660..1049)
                     /locus_tag="ACZPAQ_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HIR0903041.1"
                     /translation="MTISEKVAYLKGLAEGLNLDVEKSKEGKLISVMIGILEEVGLSI
                     EDLEENALALGEEIDTISDDLADVESVVYGEDEDEDDYFEVECPNCEEPIVIDDKALA
                     EGEVECPNCQTKFSLDLSDDTVEGDEE"
     gene            complement(1206..1769)
                     /gene="efp"
                     /locus_tag="ACZPAQ_03315"
     CDS             complement(1206..1769)
                     /gene="efp"
                     /locus_tag="ACZPAQ_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR0903042.1"
                     /translation="MISASDFRNGVTFEMDGKVMQVVEFQHVKSGKGAAFVRTKMKNI
                     ITGGVTETSFNPTAKFEQAFVERKDMEYSYNDGDLYYFMDMETYDMLPISKDMLGNAF
                     KFVKENMSCKVMSYKGSVFGVEPPNFVELEVTETDPGFKGDTATNVTKPATLETGAEI
                     KVPLFINPGDKIKIDTRTGEYLERCKG"
     gene            complement(1857..2921)
                     /locus_tag="ACZPAQ_03320"
     CDS             complement(1857..2921)
                     /locus_tag="ACZPAQ_03320"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR0903043.1"
                     /translation="MDHIAQIAGKLADHQLDAMLLASQAGERYAVGFHGEGLVLVTRE
                     GAHYSTDSRYIEAARQEVQGAQVELVTRERGHLALAAGYLQEHGLKRVGFESGAVSVD
                     ALARYERALPCTLVPAQDLPDGLRASKDGEELAAMEAAQRITDEAFQAILNFIRPGQT
                     EREVAARLVYELLRRGGERVSFDPIVAAGANGSKPHAVPGDTVIQPGMFVTMDFGCVW
                     RGYCSDMTRTVAVGKPAPEMERVYQVVLEAQQAGIAAARAGLAGREIDAAGRTVIQAA
                     GYGEAFSHSFGHSLGLEIHESPNAAPGEERIMPVGAVVSAEPGIYLPGRFGVRIEDVI
                     VLSETGCRDLTKSPKELIVL"
     gene            complement(2952..4106)
                     /locus_tag="ACZPAQ_03325"
     CDS             complement(2952..4106)
                     /locus_tag="ACZPAQ_03325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903044.1"
                     /translation="MTTIYLIRHAEAEGNLYRRIHGWYDALVTENGLRQIQALEQRFQ
                     GEHFDAVWSSDLYRTCTTARAVYEPRGLELRTDPELRELNMGDWEDQTWGQVRRTQPG
                     ELDRFNRSDPTWTAPRGEGLGELGERVERALRRIAAAHPDQTVAVFSHGTAIRQALAR
                     IKGIPPEEWHGLRHSDNTAVSCLKWDGAAFQLAFEGDNSHLDPAISTLARQSWWRRDG
                     KRAEDVNLWFRPLDLERESQTYYEARREAWITVHGEDTPFDGESFLQDARLHCGRTPW
                     GVTCAMAGDRMAGLIQLDPERYAKDGAGYIPFCYIVPQLRAQGLGVQLIGQAVSFYRP
                     LGRQYLRLRCASYNTPAQHFYQRYGFYKIGEEQGSRVPLDILEKHIGYDR"
     gene            complement(4171..>4548)
                     /locus_tag="ACZPAQ_03330"
     CDS             complement(4171..>4548)
                     /locus_tag="ACZPAQ_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR0903045.1"
                     /translation="VAGAGADALSLINTLRGMRIDVNTRRPVLKMNTGGLSGPAVLPV
                     AVRMVWEVAQAVDLPILGMGGVSKGEDAAQMMLAGASAVAVGTALFADPYAPIRVRDE
                     LAALAAGQGLERAAELIGGVKPW"
BASE COUNT          713 a         1609 c         1361 g          865 t
ORIGIN      
        1 ggtatgcctt tatcgggccc acggtgcccg agctgactca cgccccacgc tccggcgggg
       61 acaggcagat ctacatctct ctggggactg tcaacaaccg cagccgctcc ttttatcgcg
      121 cctgcctgga ggccctgcgg agctgggagg gggtacgggc cgttctctct ctgggcggtg
      181 ggtgcaggcc ggaagagctg ggagatgttc cgcccaatgt ccaggtcctt ccccaggtag
      241 accagctggc cgtcctgtct cagtccgacg cctttctctc ccactgcggc atgaacagcg
      301 tcagcgagag cctctacctg ggggtccccc tggtcctctt cccccagcag gaggagcagg
      361 gcatggtggc ccgccgggtc caggagttgg gggcgggcgt cccgctgccc gctcccaccc
      421 ccgccgccat ccatcaggcc ctctccctcg tcctggctga tcctgccttt cagcacagcg
      481 cccagcacat ctctgaaggg ttccgcgccg ccggcggcta ccggcgggga gcggataaga
      541 ttctggaagc ggccatgggc agtgctcccc tgcgctgaac acctgtccgg cggggcggag
      601 agaaaaacag cggcgtgacg aaatcgtcac gccgctgttt tgtgctttcg ggcagggagt
      661 tactcctcgt ctccctccac cgtgtcatct gacaggtcca gagagaactt tgtctggcag
      721 ttggggcact ctacctcccc ctccgccagc gccttgtcgt cgatgacgat gggttcctcg
      781 caattggggc actcaacctc aaagtagtcg tcctcatcct cgtcctcgcc gtagaccacg
      841 gactccacat ccgccaggtc gtcggaaatg gtgtcgatct cctccccgag ggccagagcg
      901 ttctcctcca ggtcctcgat ggacagcccc acttcctcca ggatgccgat catcacggag
      961 atgagcttgc cctccttgga cttctccaca tccaggttca ggccctcagc cagccccttc
     1021 agataagcaa ctttctcgga aatagtcata gcagtttccc tttctgaggc taagcctcct
     1081 tcaattctgt gtcgtaccct ctgccccagt gtctctcccg acctgatttt gttcttttca
     1141 gcctctgcag aatctcatcg atacccatcc ccgctctctg cggggccgga catctgtctc
     1201 tgccatcagc ccttgcagcg ctccaggtac tctccggtgc gggtgtcgat cttgatcttg
     1261 tcaccagggt tgatgaacag gggcaccttg atctcggcgc cggtctccag ggtggcgggc
     1321 ttggtcacgt tggtggcggt gtcgcccttg aagccgggat cggtctcggt gacctccagc
     1381 tccacgaagt tggggggctc cacaccgaag acgctgccct tgtaggacat gaccttgcag
     1441 ctcatgttct ccttcacgaa cttgaaggcg ttgcccagca tatccttgct gatgggcagc
     1501 atgtcgtagg tctccatgtc catgaaatag tacagatcgc cgtcgttgta gctgtactcc
     1561 atgtccttgc gctccacgaa agcctgctca aacttggcgg tggggttgaa ggaggtctcg
     1621 gtcacaccgc cggtgatgat gttcttcatc ttggtgcgga caaaggcggc gcccttgccg
     1681 gacttgacgt gctggaactc gacgacctgc atgaccttgc cgtccatctc aaaggtcacg
     1741 ccgttgcgga aatcactggc ggaaatcatt gccataggaa tcatcctcca aatgttttta
     1801 tgaactgata tattctaccc tttttttctg gatttgacaa gaggttttct gtaaaatcac
     1861 aggacgatca gctcttttgg cgactttgtc aggtcacggc atcccgtttc cgacaggacg
     1921 atcacgtcct cgatgcgcac gccaaaacgc ccgggcagat agatgcccgg ctcggcggag
     1981 accactgccc ccacgggcat gatccgttcc tcccccggcg cggcattggg ggactcgtgg
     2041 atctccagtc ccagggagtg gccgaagctg tgggaaaatg cctccccata ccccgccgcc
     2101 tggatcaccg tccggcctgc ggcatcgatc tcccgcccgg ccagcccggc ccgggcggcg
     2161 gcgatgccag cctgctgggc ctccagcacc acctgatata cccgctccat ctccggtgcg
     2221 ggctttccca ccgccacggt gcgggtcatg tcggagcagt agcccctcca cacacagccg
     2281 aaatccatgg tgacgaacat cccaggctgg atcaccgtgt ctccggggac ggcatggggt
     2341 ttggagccat tggcgccggc ggccacgata gggtcgaagg agacccgctc cccgccccgg
     2401 cgcagcagct catacaccag ccgggcggcc acctcccgct ccgtctgccc cgggcggata
     2461 aaattcaaaa tcgcctgaaa tgcctcgtcg gtgatgcgct gggccgcctc catagcggcc
     2521 agctcctctc cgtccttgga cgcccgcagg ccgtccggca ggtcctgagc cgggaccagg
     2581 gtgcagggca gggccctctc ataccgggcc agggcgtcca cactcacggc cccgctctca
     2641 aagcccaccc gcttcaggcc gtgctcctgg agatagccgg cggccagggc cagatggccc
     2701 cgctcccggg tcaccagctc cacctgagcc ccctgcacct cctgccgggc cgcctcgatg
     2761 tagcggctgt cggtggaata gtgggccccc tcccgggtca ccagcaccag gccctccccg
     2821 tggaagccca cggcgtaccg ctccccggcc tgactggcga gcagcatggc gtccagctga
     2881 tggtccgcca gttttcccgc gatctgggca atatggtcca tcccatgtcc ctccttttga
     2941 acggccgcag ctcagcggtc atagccgatg tgcttctcca ggatatccag cggcacccgg
     3001 ctcccctgct cctcgccgat cttatagaag ccgtaccgct gataaaagtg ctgggcgggg
     3061 gtgttgtagc tggcacagcg caggcgcaga tactgccgcc ccagggggcg gtagaaggac
     3121 accgcctgcc cgatgagctg cacccccagc ccctgggccc gcagctgggg cacaatgtag
     3181 cagaatggga tatagccggc cccgtccttg gcataccgct ctgggtccag ctggatcagc
     3241 cccgccatcc ggtcccccgc catggcgcag gtcacccccc atggggtgcg gccgcagtga
     3301 aggcgggcat cctgaagaaa gctctcccca tcaaaggggg tgtcctctcc gtggacggtg
     3361 atccatgcct cccgccgggc ctcgtaatag gtctggctct cccgctccaa gtccaggggc
     3421 cggaaccaca ggttcacgtc ctccgcccgc ttcccgtccc tccgccacca ggactggcgg
     3481 gccagtgtgg agatagcggg gtccaggtgg gagttgtccc cctcgaaggc cagctggaac
     3541 gccgctccgt cccacttcag gcaggagacg gcggtgttgt cgctgtggcg caggccgtgc
     3601 cactcctccg gcgggatgcc cttgatccgc gccagagcct gccggatggc ggtgccgtgg
     3661 ctgaacaccg ccaccgtctg gtccgggtgg gcggcggcga tgcggcgcag ggcccgctcc
     3721 acccgctccc ccagctcccc cagcccctct ccacgggggg ccgtccaagt ggggtcgctg
     3781 cggttgaagc ggtccagctc cccgggctgg gtgcggcgca cctggcccca ggtctggtcc
     3841 tcccagtccc ccatgttcag ctcccgcagc tccgggtcgg tacgcagttc caggcccctg
     3901 ggctcataga cagcccgggc cgtggtgcag gtgcggtaca ggtcgctgga ccacaccgcg
     3961 tcgaagtgct ccccctggaa gcgctgctcc agggcctgga tctgcctgag accgttctcg
     4021 gtcaccaggg cgtcgtacca gccgtggatg cggcggtaca ggtttccctc cgcctcagcg
     4081 tggcggatca gatagatggt cgtcatattc ggtattcctt cttttgagtt tcgcggcacg
     4141 ccccaccggg gctccgcctg ccgcgtatcc ttaccagggc ttcactccgc cgatgagctc
     4201 cgccgcccgc tccaggcctt ggccggccgc cagggcggcc agctcgtccc gcacccggat
     4261 cggggcgtag gggtcagcaa agagggcggt gcccaccgcc acggcggagg ccccggccag
     4321 catcatctgg gccgcgtcct cccccttgga caccccgccc atccccagga tgggcaggtc
     4381 caccgcctgg gccacctccc agaccatccg caccgccaca ggcaggacgg cggggccgga
     4441 caggccgccg gtgttcatct tcagcacagg gcgccgggtg ttcacgtcga tgcgcatccc
     4501 ccgcagggtg ttgatgagcg agagagcgtc cgcccccgcc ccggccac
//
LOCUS       DBMDSJ010000092         4501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5192_length_4501_cov_1.890682, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000092 DBMDSJ010000000
VERSION     DBMDSJ010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5192_length_4501_cov_1.890682"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(86..787)
                     /locus_tag="ACZPAQ_03335"
     CDS             complement(86..787)
                     /locus_tag="ACZPAQ_03335"
                     /inference="COORDINATES: protein motif:HMM:NF024881.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR0903046.1"
                     /translation="MNQPCPMIQDLLPLYVDQICSQESRRAVEEHVENCPLCRARLEE
                     LRREDPLPPPEQDREQSKARVLAGVKRQLRRKKLCFSARVLAGAAALGVAVWLLVFRF
                     QTPIPYSQDIRIQEVEPAFHMKYLGRDYAGLEVLFRVTEEGGEIIRRVYLCYTGSLWT
                     RYLSPGSPYHESVGINNGIVLDTRHGTVIPLPFDGGADQVWYLAGDLEELEDLDEASF
                     QAQAGDAVLLWERQG"
     gene            complement(787..1248)
                     /locus_tag="ACZPAQ_03340"
     CDS             complement(787..1248)
                     /locus_tag="ACZPAQ_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903047.1"
                     /translation="MIDFEEVYQDYLEDVYRFSLALCRDPVLAEELTQETFFRALEHI
                     GQFRGECTLRVWLCQIAKHAWLTHCRRQRRREPEPELREEGPEERLAEKETAMEVHQV
                     LHSLPEPYREVFALRVLGELSFREIGALFHKTESWARVTFHRARLKIKEAL"
     gene            complement(1351..2157)
                     /locus_tag="ACZPAQ_03345"
     CDS             complement(1351..2157)
                     /locus_tag="ACZPAQ_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR0903048.1"
                     /translation="MGKFDGVLLVSDFDDTLYDSHCQVPRRNLEALDRFLAQGGRFTV
                     ATGRAHRTFAPYVHLAPVNAPVVLSNGSAIYDFQKDEFLVQTYLSPSAPQDLDGVMEA
                     FPQLGMEAYHGEDIYVFRPNWITDLHMKKVGTDYTVCPIREMPTPWTKAILQQDHGVL
                     KEVQSWLLDRFPRRYEAIFSNHVYLEITDHGCNKGGMVARLAQMLGIARENIYCVGDN
                     QNDIPMMALSAVPFAPANCAQEVKDWGAHILCHCDDGVIGDIMEILEQRY"
     gene            complement(2168..2728)
                     /locus_tag="ACZPAQ_03350"
     CDS             complement(2168..2728)
                     /locus_tag="ACZPAQ_03350"
                     /inference="COORDINATES: protein motif:HMM:NF021062.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIR0903049.1"
                     /translation="MNRHSRSTRALVEAALMIALATILGYFRLYRFPQGGSVDFAMVP
                     ILIYCLRWGPAWSLSCCFVNGVLQFFLGGGIALSWQSMLLDYVVAYTLVFLCGFAAGK
                     KLGWLWGTILAGLGRWVSLTLSGGLLWYMYMPEEFLGLPMVDPWVYSMIFNGVLIVLV
                     TVVNLIVLGIFQSVPALRQKLLTKQN"
     regulatory      complement(2797..2911)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3105..4247
                     /locus_tag="ACZPAQ_03355"
     CDS             3105..4247
                     /locus_tag="ACZPAQ_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014893.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR0903050.1"
                     /translation="MKKLQFAMVGGGNGALIGDIHRIGAQFDDLAELKAGCFSRHWDK
                     NLEAGEKWGVEPGRIYPDYHTMLEEEAKRTGEDRLDFVVVATPNNTHYEIVKDIIAHG
                     FHIVCDKPVTFTSRESEELKALAQEKDLLFGVSYSYAHYAILEQAKKMIADGVLGKIH
                     TIAAEYPQEWVGLNTALRGKDGLTWRVDPAICGPSGVIADIGTHVEYLISKVTGLKPV
                     RVLARLRSIPDYVPVESDAQVLIDYDNGAAGIFWTSFAMVGKSCCMKVRIYGDKGGME
                     WNAHDFMELRVDIAGQPTQIWSANYDYCDPQARKSCRLPKGMPEAFHSLFANFYRGYC
                     SHLLSRKEGKDERPFPYSDIDDGIQGLKFIEACLKSQEDGNVWTQV"
BASE COUNT          917 a         1427 c         1307 g          850 t
ORIGIN      
        1 ggcgcctttc atccccagga taccctgacc cgggcggagg gggaggccct tctctctctt
       61 ctcccctgac cccggccctc ttgtgtcacc cctgcctctc ccagagcagc accgcgtctc
      121 cggcctgcgc ctggaaggag gcctcgtcca gatcctccag ctcctccagg tccccggcca
      181 gataccagac ctgatccgcc ccgccgtcga aggggagggg gatcaccgtc ccgtgtctgg
      241 tgtccaggac gatgccgttg ttgatcccca cactttcgtg gtatggggag ccgggagaga
      301 gataccgggt ccacaggctg ccggtatagc agagatagac ccttcggatg atctccccgc
      361 cctcctccgt cacccggaac aggacctcca ggccggcgta atcccggccc agatatttca
      421 tgtggaaagc cggctccacc tcttggatgc ggatatcctg tgaatagggg atgggtgtct
      481 ggaagcgaaa gaccaacagc cagaccgcca cgcccagggc tgccgcaccg gccagcaccc
      541 gagcggagaa acacagcttt ttccttctca gctgcctttt taccccggcc agcacccggg
      601 ccttcgactg ctcccggtcc tgctctgggg ggggcagcgg gtcttcccgg cgcagctcct
      661 ccagccgggc acggcagagg ggacagttct ccacatgctc ctccaccgcc cggcggctct
      721 cctggctgca gatctggtcc acgtacaggg gcagcagatc ctggatcatg ggacagggct
      781 gattcatcat aatgcctcct tgatttttag tctggcccgg tgaaaagtga cccgggccca
      841 gctctccgtc ttatggaaca gtgctcctat ctcccggaag gacagctccc ccagcacccg
      901 cagggcaaag acctcccgat agggctcggg caggctgtgg agtacctggt ggacttccat
      961 ggcagtctcc ttctccgcca gccgctcctc cggcccctcc tcccggagct ccggctccgg
     1021 ctcccgcctt ctctgcctgc ggcagtgggt cagccaggcg tgcttggcga tctggcacag
     1081 ccagacccgc agggtgcact ccccccggaa ctggccgata tgctccagcg cccggaaaaa
     1141 ggtctcctgg gtcagctcct ccgccagtac cgggtcccgg cacagggcca gagaaaatcg
     1201 gtagacgtcc tccagatagt cctggtaaac ttcttcaaaa tcgatcacgc cctcacctcc
     1261 ctttccacag ataagacggc atctccccct tttcgttaca aaagatccac cgccattttt
     1321 tggcggtgga tctttctctt tccaatattt ttaatatcgc tgctccagga tctccatgat
     1381 atccccgatc acgccgtcgt cgcagtgaca caggatatgg gctccccagt ccttcacctc
     1441 ctgggcacag ttggcagggg caaaggggac ggcggacagt gccatcatgg ggatgtcgtt
     1501 ctggttgtct cccacgcagt agatattttc tcgggcgatc cccagcatct gggccagccg
     1561 ggccaccatt ccccccttgt tgcacccatg gtcggtgatc tccaggtaca cgtggttgga
     1621 aaaaatagcc tcataccggc gggggaagcg gtccagaagc cagctctgca cctccttcag
     1681 caccccgtgg tcctgctgga ggatggcttt ggtccagggc gtaggcatct cccgaatggg
     1741 acagacggtg tagtcggtgc ccaccttttt catgtgcagg tctgtgatcc agttgggtcg
     1801 gaacacatag atatcctccc cgtggtaggc ctccatcccc agctgtggaa atgcctccat
     1861 caccccatcc aagtcctgag gcgcactggg agacagatag gtctgcacca gaaattcgtc
     1921 tttttggaag tcatagatgg ccgacccgtt ggagaggacc accggggcat tgacgggggc
     1981 cagatggaca tagggagcga aggtccggtg ggcgcggccg gtggccacgg taaaacgccc
     2041 cccctgggcg aggaagcggt ccagggcctc caaattccgc cggggcacct gacagtggct
     2101 gtcatacagc gtgtcgtcaa aatcactgac cagcaggaca ccgtcgaatt ttcccaaaga
     2161 aaaaacctca attctgtttc gtcagcagct tctgccgcag agcggggacg gactgaaaga
     2221 tccccagaac gatcaggttg accacagtca cgagaacgat cagcacgccg ttaaagatca
     2281 tggagtagac ccaggggtcc accatgggca gtcccaaaaa ttcctccggc atatacatat
     2341 accacagcag gccgccggac agggtgagag atacccaccg tcccagcccg gccaggatgg
     2401 tgccccacag ccagcccagc ttcttccccg cggcaaagcc gcagaggaac accagcgtat
     2461 aagccaccac atagtccagc agcatggact gccaggatag agcgatccct ccgcccagga
     2521 agaactggag cacaccgttg acaaagcagc agctcaggct ccaggccggg ccccagcgca
     2581 ggcagtagat caggatgggc accatggcaa agtccacaga ccccccctgg ggaaagcggt
     2641 acagccggaa gtagcccagg atggtggcca gcgcgatcat cagcgcggcc tccacaaggg
     2701 cgcgggtaga gcgggaatgt cggttcatcg tcgtttcctc ctgaaatcaa gataccgcag
     2761 cacatccgaa cgggtgcgtg cggtatgaag aggacgacat atggatcgtt cactccctac
     2821 gccggcatta cccggatcag gtcaagggac tggagctgcg attcgcttcc atctcagccg
     2881 ggaatagctc ccagcgcccc tgtgttcgat tgtctgcggt catggttatt gtatcccaaa
     2941 gggacgggcc ttgtcaagcg tgctcttttc tttatccact ccccctcttg tatgtgccct
     3001 ttgaatgggt ccatgactgg atactattag acacctgccg ccctgcgtgg tatcctgaag
     3061 acgatttctt gtacacttta cacaatccgt caggaggtct ctctatgaaa aagctgcaat
     3121 tcgcaatggt cgggggcggg aacggcgccc tgatcggaga tatccaccgc atcggggccc
     3181 agttcgacga cctggccgaa ctgaaggcgg gctgtttctc ccgccactgg gataaaaatc
     3241 tggaggccgg agagaaatgg ggggtggagc ccggccgcat ctatcccgac taccacacca
     3301 tgctggagga ggaagccaag cgcaccgggg aggaccggct ggactttgtg gtggtggcca
     3361 cccccaacaa cacccactac gagatcgtca aggacatcat tgcccacggc ttccacattg
     3421 tctgcgacaa acccgtcacc ttcaccagcc gggagtcgga ggagctgaag gccctggccc
     3481 aggaaaaaga tcttctgttc ggcgtgtctt acagttatgc ccactacgcc attctggagc
     3541 aggccaaaaa gatgatcgct gacggggtgc tgggaaagat ccacaccatc gccgccgagt
     3601 atccccagga gtgggtgggg ctgaacactg ccctccgggg aaaggacggc ctgacctggc
     3661 gggtcgatcc ggccatctgc ggcccctctg gagtgatcgc ggacatcggc acccatgtgg
     3721 agtatctcat cagcaaggtc accggcctca agcccgtgcg ggtgctggcc cgcctgcgct
     3781 ccatcccgga ttatgtgccg gtggagagcg atgcccaggt gctcatcgac tacgacaacg
     3841 gggcagcggg catcttctgg acctccttcg ctatggtggg caagagctgc tgcatgaagg
     3901 tgcgcatcta cggcgacaaa gggggcatgg agtggaacgc ccacgatttc atggagctcc
     3961 gggtggacat cgccggacag cccacccaga tctggtcggc caattatgac tactgtgatc
     4021 cccaggcccg gaagtcctgc cgcctcccca aggggatgcc ggaggccttc cactccctgt
     4081 tcgccaactt ctaccggggt tactgttccc atctgctgtc ccggaaggag ggcaaagatg
     4141 agcggccctt cccctactcc gatatcgatg acggcatcca ggggctgaaa tttatcgagg
     4201 cctgcctgaa gagccaggag gacgggaacg tctggaccca ggtgtgagca aaggatatca
     4261 aaaacagggc ccgggaaaac cacagggttt tcccgggctc tcttctgacc ctagaaaatt
     4321 ttccacagga ggccggtggg ccctgtggaa tggtcgatgt gaaacggcgg gcgcgctttg
     4381 cgcgccccta cgcaaagacc gaaggggacc ccgtaggggc ggcttccagc cgcccgaaaa
     4441 gccttccccc cacatggggg gaaggtgtca cggcatatgc cgtgacggat gaggggtctt
     4501 t
//
LOCUS       DBMDSJ010000093         4489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5211_length_4489_cov_2.016864, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000093 DBMDSJ010000000
VERSION     DBMDSJ010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_4489_cov_2.016864"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /gene="rdgB"
                     /locus_tag="ACZPAQ_03360"
     CDS             <1..463
                     /gene="rdgB"
                     /locus_tag="ACZPAQ_03360"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR0903051.1"
                     /translation="TFEENSLLKAGAVMEASGLPAIADDSGLCVDALNGAPGVYSARY
                     GGPGLDDPGRVRLLLENMRGQMPRTCRFVSVITCCFPNGDVLSARGECEGTVAFTPIG
                     EGGFGYDPVFFVPPLKKTFAQLTAEEKNAISHRGKALEAFQVKLREYLEKS"
     gene            580..921
                     /locus_tag="ACZPAQ_03365"
     CDS             580..921
                     /locus_tag="ACZPAQ_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HIR0903052.1"
                     /translation="MDLTSKQRAQLRGLANGIDTILIVGKEGIGDNLVKQANDALEAR
                     ELIKGRVLENALMSAREAAEALAPLTRSEVVQVIGTKFVLYRQSHRKDKKDKIVLVEG
                     KKRESVPQREP"
     gene            996..1418
                     /locus_tag="ACZPAQ_03370"
     CDS             996..1418
                     /locus_tag="ACZPAQ_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903053.1"
                     /translation="MKRRKWRAALTALLLLAAVGGLLWCFFPRSMMEGELYCVTLWDG
                     QARVDLTDRADREAVTERIARCRRVYGGKVGAYQLDRYPLELGFSYRGGSWYLIAGPE
                     GAFAYDSGERLNWRILDGEQLWRDLASALPSSSEPGER"
     gene            1442..2650
                     /gene="nadD"
                     /locus_tag="ACZPAQ_03375"
     CDS             1442..2650
                     /gene="nadD"
                     /locus_tag="ACZPAQ_03375"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR0903054.1"
                     /translation="MKIGIYGGTFNPPHLGHLEAARTAVKVLELDKLILIPAAVPPHK
                     ALPEGTPEGTHRLAMTERMADALSMPKVAEVSRMELDREGKSYTADTLRAIRAQYPDA
                     QLWLLMGTDMFLTLHLWHDPEVITQLAGICAFGRTEQDGEELFAPQREFLSQKYQAQI
                     VTITLPGLVDVSSTRLRELLARGEGQEYLLPQVYGYILMNRLYGTHADLKRLELPELR
                     ACSYSMIRAKRVPHVMGVEEEAVRLAERWGGDEGMARRAAILHDCTKYLELDDQLRLC
                     RQYGVELDELEQQAVKLLHAKTGACIARDIFGEPDEVYQAIFWHTTAKADMTLLEKII
                     YIADYIEPNRDFEGVEELRRLAYEDLDRALLLGVETTIQEMRDRRLPIHTNTLRARDW
                     LREHGTKTEE"
     gene            2667..4289
                     /locus_tag="ACZPAQ_03380"
     CDS             2667..4289
                     /locus_tag="ACZPAQ_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR0903055.1"
                     /translation="MSTENRGRREQHRERRSLGEKFQQWRQQRREWIAGMTRGQRIRH
                     RLLQAAVVLAIAIIAVWAVMSAWIRVPKVPDPPTAGPDTTQQPGEGEDIQFTGAELPN
                     VAKSGRKEGYYTFLVVGRDVASGLTDTILLFTFDTNNKSLNAISLPRDTMINTSAKGG
                     SLKRINVVYNRNRGSSDLPEAQRVENGMTALKQEVSRLTGIYPDFYVMVEWEAIGELV
                     DALGGVYFDVPFDMNYDDPYQDLHIHQEAGYRKLSGEDAMEVIRWRKNNGEPSPGDVG
                     RIQIQQDFLSAVLDKCLQPATLLKAPALAQVFLNNVTTDLTVGNILAFAQLAIGMDPE
                     SDVKFSTMPYTGVMYDRASLVLPVEKELLEILNSGMNPYVDQIQSSDLQLMYKKSGGG
                     YGVTTGTLLDSSMAYAGSSSSSSGTQTTQETEEPETEQPGETDAGTETPGTTPPEGTE
                     TGTGEPGSTGSETGEPGTQGPEGETPDTDDPGAVQPEGPGQGEGAGASQPEQDPGGGN
                     GGASQSEPSQPGTEGESGGSDLPPEWLLPDVA"
     gene            4329..>4489
                     /locus_tag="ACZPAQ_03385"
     CDS             4329..>4489
                     /locus_tag="ACZPAQ_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA];
                     GO_process: GO:0090071 - negative regulation of ribosome
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsfS/YbeB/iojap family protein"
                     /protein_id="HIR0903056.1"
                     /translation="MTSRESAIVLAKALDSKKGMEIKVLKTEELTTLADYFVICTASS
                     TTQIKALSDV"
BASE COUNT          934 a         1306 c         1567 g          682 t
ORIGIN      
        1 cacctttgaa gagaattccc tgctgaaggc cggggcggtc atggaggcca gcggtctgcc
       61 tgccatcgcc gacgactccg ggctgtgcgt ggacgccctc aacggcgcgc cgggggtgta
      121 ctccgcccgg tacggagggc ctgggctgga cgacccggga cgggtgcgcc tgctgctgga
      181 gaacatgcgg ggccagatgc cccggacctg ccgctttgta tcggtgatca cctgctgctt
      241 ccccaacgga gacgtgctct ctgccagagg agaatgtgag ggcaccgtgg ccttcacccc
      301 cataggggag ggaggcttcg gctatgaccc ggtgtttttt gtgccgccgc tgaaaaaaac
      361 ctttgctcag ctgacggcgg aggagaagaa cgccatctcc caccggggga aggctctgga
      421 ggcgtttcag gtgaaactga gggagtatct ggagaaaagc tgacgcccgg ccccaaatgg
      481 gagcccagcg gagcgggtcc catttggaga ggacgagcaa cggaatagag cggatgtccg
      541 ccgggaggcg gacggagcgg aatggagttt gtgaggacga tggatttgac aagcaaacag
      601 cgcgcccagc tgcgcggtct ggccaacggg atcgacacca tcctgatcgt gggcaaggag
      661 ggcatcggtg acaatttggt caagcaggcc aacgatgccc tggaggcccg agagctgatc
      721 aagggcaggg tgctggaaaa cgccctgatg tcggcccggg aggcggcgga ggcgttggcc
      781 cccctgacca gaagcgaggt ggtccaggtg atcgggacca aatttgttct ctaccgccag
      841 agccaccgga aggacaaaaa ggacaagatc gtgctggtgg agggaaagaa gagagaaagc
      901 gtaccccaga gggagccata aaaaacagaa ttgtctctga ggagcggcag caagaaggac
      961 tgaagcggaa gaagggctga gaggaggctt gtctcttgaa acggagaaaa tggcgtgcgg
     1021 ccctgaccgc cctcctgctt ctggctgcgg tggggggact gctctggtgc ttctttcccc
     1081 gctccatgat ggagggagag ctgtactgtg tgaccctgtg ggacggacag gcgagggtag
     1141 acctgacgga ccgggcagac cgggaagctg tgacagagcg gatcgcgcgc tgccggcggg
     1201 tctatggcgg gaaggtgggc gcctaccaac tggataggta ccccttagag ctgggcttca
     1261 gctaccgggg gggcagctgg tatctgatcg ccggaccgga gggcgccttc gcctatgaca
     1321 gcggggagcg cttaaactgg cgcattctgg acggagaaca gctgtggcgg gatctggcat
     1381 ccgctctccc gtccagctca gagccggggg agcggtgaac ttggaaagga agggtgtctg
     1441 tatgaaaatt gggatctatg gagggacctt caacccaccc cacctggggc atctggaggc
     1501 ggcccggacg gcggtcaagg tgctggagct ggacaagctg atcctgatcc cggcggcggt
     1561 cccgccccac aaggccctgc cggaggggac gccggagggc acccaccgtc tggccatgac
     1621 cgagcggatg gcggacgccc tatccatgcc gaaggtggcg gaggtatccc gcatggagct
     1681 ggaccgggag ggcaagagct ataccgccga taccctccgg gccatccggg ctcagtaccc
     1741 ggacgcccag ctgtggctgc ttatggggac agacatgttc ctcaccctcc acctgtggca
     1801 cgacccggag gtcatcaccc agctggcggg gatctgcgcc tttggcagaa cagagcagga
     1861 cggagaggag ctgttcgccc cccagaggga gttcctctcc cagaagtatc aggcccagat
     1921 cgtcaccatc accctgccgg gactggtgga tgtgtcctct acccggctgc gggagctgct
     1981 ggcccggggg gagggacagg agtatctgct cccccaggtg tacggctata tcctgatgaa
     2041 ccggctgtac ggcacccacg cggacctgaa gcggctggag cttccggagc tgcgggcctg
     2101 ttcctactcc atgatccggg ccaagcgggt cccacatgtt atgggcgtgg aggaggaggc
     2161 ggtgcgcctg gccgagcggt ggggcggaga cgaggggatg gcccgccggg ccgctatcct
     2221 ccacgactgc accaagtatc tggaactgga cgaccagctc cgcctgtgcc ggcagtacgg
     2281 ggtggagctg gatgagctgg aacagcaggc ggtgaagctg ctccacgcca agacgggggc
     2341 ctgtatcgct cgggacattt ttggggaacc tgatgaggtg taccaggcca tcttctggca
     2401 taccacggcc aaggcggaca tgaccctgct ggaaaagatc atctatatcg ccgattacat
     2461 cgagcccaac cgggacttcg agggggtgga ggagctgcgc cggctggcgt atgaggacct
     2521 ggaccgggcc ctgctgctgg gggtggagac caccatccag gagatgcggg accgccggct
     2581 ccccatccac accaataccc tccgggcccg ggactggctg agagagcacg ggacaaaaac
     2641 agaggaatga caagaggaac gagcagatga gcactgagaa cagaggacgc agagagcagc
     2701 atcgggagcg gcgctccctg ggagagaagt ttcaacagtg gcggcagcag cgccgggagt
     2761 ggatcgccgg gatgacccgg ggacagcgca tccggcaccg cctgctccag gcggcggtgg
     2821 tcctggccat cgccatcatc gcggtctggg cggtgatgag cgcctggata cgggtgccca
     2881 aggtgcctga cccgccgacg gccggcccgg acaccaccca gcagcccggg gagggagagg
     2941 acatccagtt cacgggggca gagctgccca atgtggccaa gagcggacgg aaggagggct
     3001 actacacctt cctggtggtg gggcgggacg tggccagcgg cctgacggac accatcctcc
     3061 tctttacgtt cgacaccaac aacaagagcc tgaacgccat cagcctgcca cgggacacta
     3121 tgatcaacac cagcgccaag ggcggttcgc tgaagcggat caacgtggtg tacaaccgca
     3181 accggggctc ctccgacctg ccggaggccc agcgggtgga gaacggcatg actgccctga
     3241 agcaggaggt gagccgcctg accggcatct accccgactt ctatgtgatg gtggagtggg
     3301 aggccatcgg cgagctggtg gacgccttgg gcggagtgta cttcgacgtc ccatttgata
     3361 tgaactacga cgatccctat caggacctgc atatccacca ggaggctggc taccggaagc
     3421 tgagcgggga ggacgccatg gaggtcatcc gctggcggaa gaacaacggg gagccctctc
     3481 cgggcgatgt gggacgtatc cagatccagc aggacttcct ctccgccgta ctggacaagt
     3541 gcctccagcc agcaaccctg ctgaaggccc cggcactggc ccaggtgttc ctgaacaacg
     3601 tgaccaccga cctgacagtg ggcaatatcc tggccttcgc acagctggct atcggcatgg
     3661 acccggagag cgatgtgaaa ttttccacta tgccctatac cggggtcatg tatgacaggg
     3721 cctcactggt gctgccggtg gagaaggagc tgctggagat cctcaacagc ggcatgaacc
     3781 cctatgtgga ccagatccag tccagcgacc tccagctgat gtacaaaaag agcggcgggg
     3841 gatacggggt caccaccggc accctgctgg actccagcat ggcttacgcc gggtcctcca
     3901 gctcctcctc ggggacacag actacccagg agacagagga gcctgagaca gaacagccag
     3961 gggagacgga cgccgggacg gagacccccg gcaccacgcc cccggagggc acggagaccg
     4021 gaaccgggga acccggcagc accggctcgg agactgggga gcccggcacg caagggccgg
     4081 agggagagac tcccgatacg gatgaccccg gggctgtgca gcctgaaggg cccggccagg
     4141 gggagggagc tggagcctcc cagccggagc aggaccctgg cggcgggaac gggggtgcgt
     4201 cacagtccga accctcccag cccggcacag agggggagag cggcggcagc gatctgcccc
     4261 cggagtggct gctgcctgat gtggcgtgat gcctgataca gaatacaaaa aggaaaggaa
     4321 ttttgcccat gacatcccga gagagtgcga tcgtgttagc caaagccctg gacagtaaga
     4381 agggcatgga gatcaaggtg ctgaagacgg aggagctgac caccctggcg gactacttcg
     4441 tcatctgtac tgcctcctcc accacccaga tcaaggccct gtctgacgt
//
LOCUS       DBMDSJ010000094         4441 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5267_length_4441_cov_1.758525, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000094 DBMDSJ010000000
VERSION     DBMDSJ010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5267_length_4441_cov_1.758525"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            117..638
                     /gene="nrdG"
                     /locus_tag="ACZPAQ_03390"
     CDS             117..638
                     /gene="nrdG"
                     /locus_tag="ACZPAQ_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR0903057.1"
                     /translation="MNYADIRPIDVANGPGVRVSLFVSGCPHRCPGCFNPETWDFQYG
                     QGFGEEQVRSILDLLRPGYIRGLTLLGGEPMAPANQPEVLSLVRRVRQELPGRDIWCY
                     TGYLYEELAAGRVGPLDRELLHELDVLVDGPFLLEQKDIRLRFRGSANQRILDVPRSL
                     AAGEALWWDSPQD"
     gene            775..1281
                     /locus_tag="ACZPAQ_03395"
     CDS             775..1281
                     /locus_tag="ACZPAQ_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR0903058.1"
                     /translation="MDNIILIGMPGSGKSTVGVLLAKALGYGFLDTDLVIQQREGLLL
                     QDILDQFGVKHFLQVEEEAIRTVDCRCTVIAPGGSAVCREGTMAHLKELGPVVYLRVP
                     MEELTSRIQNLATRGIAMEPGQTLADVLAQRAPLYERYADLTVDCLPGQTLAQTTQMV
                     LALLNGRR"
     gene            1367..3199
                     /locus_tag="ACZPAQ_03400"
     CDS             1367..3199
                     /locus_tag="ACZPAQ_03400"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR0903059.1"
                     /translation="MTFRDLGLIAPILSALEEQGYTRPSPIQEKAIPPALEGRDVLGC
                     AQTGTGKTCAFAAPILQRLSAAPVKGRPIRALILTPTRELAIQIGDSFQAYGSHLSLR
                     HAVIFGGVGQNPQVEALNRGVDILVATPGRLMDLHDQGFVHLEQLEIFVLDEADRMLD
                     MGFIHDVKKILKWLPARKQTMLFSATMPPEIADLVNSLLRSPVRVAVDPVSSPVEAIR
                     QSVYFVDKGNKTRLLAQLIQELAVKNALVFTRTKHGANKVARELSKYGITAAAIHGNK
                     SQTARQQALADFRAGKLQALVATDIAARGLDIEELSHVFNYNLPEVPETYVHRIGRTG
                     RAGRGGEAIAFCDFSEKPLLREIEKLTHRSIPVVEEHPWPMQVFELPKKDKNGRTINE
                     EDAEARAAARELRREREAARQAAAQEKQEKSAQASAPSPEATVEGGEKKKRRRRRSKS
                     KGEAPAAPAESPLPAQAPARPKLTRPGTRLETGSAMPCTEFDRPDPLAGDRIMDATAR
                     LLAPRRLTLSPVSSREEHGRPRKRSAGQAAQPRQKSEGQKGRSAHTAPQAESRKPAPS
                     KHKKSSQGRGQDRRRSFQDPPRPTGHPKDSTEQESLMKPYYLDI"
     gene            3232..>4441
                     /locus_tag="ACZPAQ_03405"
     CDS             3232..>4441
                     /locus_tag="ACZPAQ_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyryl-CoA:acetate CoA-transferase"
                     /protein_id="HIR0903060.1"
                     /translation="MDYQAMYQQKLTTAEEAVKVVRSGDWVDYTWCTNHPIALDKALA
                     ARKDELTDVKVRGGVTMWMPEIAKAEDAGDHFTWHSWHCSGIDRKIITKGMGYFSPMR
                     YSELPRFYRENLAPVDVVMLQTTPMDAHGNFNFGLAASHLADLMSRAKCIIVEVNENM
                     PWVYGLTGTEINIQDVTYVVEGDNPPVAQLGAGGEPTDVDRAVANLVVPQIPNGACLQ
                     LGIGGMPNTIGAMIAQSDLKDLSVHTEMYVDGFVDMAAAGKITGRHKALDKGRQVFAF
                     AAGTQKLYDYVDRNPDVMGAPVDYTNDVRVIAQIDNFISINNAIDIDLFGQINAESAG
                     IKHISGTGGQLDFAMGAYLSNGGKSFICMSSTVTGKDGTVKSRIVPTLTQGSICTDPR
                     SCGHYVVTEYG"
BASE COUNT          814 a         1544 c         1357 g          726 t
ORIGIN      
        1 gaactactgg aacgtgggca agaccaagga gatcaagtcc cgggtgctgc acctataaaa
       61 atataggatg tatctctgcc ctgcggggcg gagatacata tgagtaagag gtttttatga
      121 actacgccga catccgcccc attgacgtgg ccaacggacc gggcgtccgg gtctccctct
      181 ttgtgtccgg ctgcccccat cgctgccccg gctgcttcaa tccggagacc tgggatttcc
      241 aatatggcca gggattcggg gaggaacagg tccgctccat cctggatctg ctccgccccg
      301 gctatatccg cgggctgacc ctgctggggg gcgagcccat ggccccggcg aatcagccgg
      361 aggtgctctc cctggtacgc cgggtgcggc aggagctccc cggcagggat atctggtgct
      421 acaccggcta cctctacgag gagctggccg ccggccgggt gggtccgctg gaccgggaac
      481 ttcttcatga gctggatgtg ctggtggacg gcccctttct tctggagcag aaggacatcc
      541 gcctccgttt ccgtggcagt gccaaccagc gtatcctgga tgttccccgg tccctggcgg
      601 ccggggaggc cctctggtgg gacagccccc aggactgaat ccagcggcag ccctgagctt
      661 tcagggctgc cgctcctgtt tttatcctgc tgggcgtaca tttccccttc ctttttccgg
      721 gtttttgtgt taaactaatt cggatatcag tctgtacaga ggtaggatca cgctttggac
      781 aacatcattc tcatcggcat gcccggctcg ggcaagagca ctgtgggcgt tcttctggcc
      841 aaagccttgg gctacggctt tctggacacc gacctggtga tccagcagcg ggagggcctg
      901 ctgctccagg acatcctgga ccagttcggg gtgaagcact tcctccaggt ggaggaggag
      961 gccatccgca ccgtggactg ccgctgcacc gtcatcgccc cagggggcag cgccgtctgc
     1021 cgggagggga ccatggccca cctgaaggag ctgggccccg tggtctatct ccgggtccct
     1081 atggaggagc tgaccagccg catccagaac ctggctacca gggggatcgc catggagccg
     1141 gggcagaccc tggcggacgt gctggcccag cgcgcccccc tctacgagcg ctatgccgat
     1201 ttgacggtgg actgccttcc tgggcagacc ttggcccaga ccactcagat ggtcctggcc
     1261 cttctgaacg gccgccggtg atcccggcag cccgccccag cccccgggga aatgggggcc
     1321 cggcgcggcg ctgaccgcgc ccggatcacg aaaaaggaag agctgaatga catttcgcga
     1381 cttagggctc atcgccccca tcctctccgc cctggaggaa cagggctaca cccggccgtc
     1441 ccccattcag gagaaggcca tcccccctgc cctggaaggg cgtgacgtgc tgggctgtgc
     1501 ccagaccggc acaggcaaga cctgcgcctt tgccgccccc atccttcagc ggctcagtgc
     1561 cgccccggtg aagggccgcc ccatccgcgc cctgatcctc acccccaccc gggaactggc
     1621 catccagatc ggtgacagct tccaggccta tggcagccat ctgtccctgc gccacgctgt
     1681 catcttcggc ggtgtgggcc agaaccccca ggtggaggcc ctcaaccggg gggtggacat
     1741 cctggtggcc acccccggcc ggctgatgga cctccacgac caggggttcg tccatctgga
     1801 gcagctggag atcttcgtgc tggacgaggc cgaccgcatg ctggacatgg gcttcatcca
     1861 cgacgtgaag aagatcctga agtggctgcc cgcccggaag cagaccatgc tcttctctgc
     1921 caccatgccg ccggagatcg ccgatctggt caactctctc ctccgctccc ctgtccgggt
     1981 ggcggtggac ccggtctcct cccctgtgga ggccatccgg cagtcggtgt acttcgtgga
     2041 caagggcaat aagacccggc tgctggccca gctgatccag gagctggcgg tgaaaaacgc
     2101 cctggtcttt acccgcacca agcacggggc caacaaggtg gcccgggagc tgagcaagta
     2161 cgggatcacc gccgccgcca tccacggcaa caagagccag accgcccgcc agcaggccct
     2221 ggccgacttc agggccggga aactccaggc cctggtggcc actgacatcg ccgcccgggg
     2281 gctggacatc gaagagctgt cccatgtctt caactacaat ctgcccgagg tgcctgagac
     2341 ctatgtccac cgaatcggcc ggaccggacg ggcgggccgt ggcggcgagg ctatcgcctt
     2401 ctgcgacttc tccgagaaac ctctgctccg ggagatcgaa aagctgaccc accgctccat
     2461 ccccgtggta gaggagcacc cctggcccat gcaggtcttc gagcttccca aaaaggataa
     2521 gaacggccgc actatcaatg aggaggacgc cgaggcccga gccgccgccc gtgagctgcg
     2581 ccgggagcgg gaggccgccc gccaggccgc agctcaggag aaacaggaga agtccgctca
     2641 ggcttctgcc ccgtccccag aggccacggt ggagggcgga gagaagaaga agcgccgccg
     2701 caggcggagc aagtccaagg gggaggcgcc cgccgccccg gcggagtccc cgctgcccgc
     2761 tcaggctccc gcccgcccca agctgacccg gcccggcacc cgcctggaga cgggcagcgc
     2821 catgccctgc actgagttcg accgccccga ccccctggct ggggaccgga tcatggacgc
     2881 cactgcccgt ctgctggctc cccgccggct gactctctcc ccggtatcct ccagggagga
     2941 gcacgggcgc ccccgaaagc gctctgctgg acaggccgct cagccccgcc agaagagtga
     3001 gggccagaag ggccgctctg cccacaccgc cccccaggcg gagagccgga agccggcgcc
     3061 gtccaagcat aaaaagtcct cccagggccg cgggcaggac cgccgccgct ccttccagga
     3121 cccgccccgt cccaccggac atcccaagga ctccaccgag caggaaagct tgatgaagcc
     3181 ctattacctg gatatctgac cgcaaataca gatgagtttg gaggttattt catggattat
     3241 caagcaatgt accagcagaa gctgaccacg gccgaggagg ccgtcaaggt ggtcagatcc
     3301 ggcgactggg tggactacac ctggtgcacc aaccacccca ttgccctgga caaggctctc
     3361 gccgcccgca aggatgagct caccgatgta aaggtccggg gcggcgtcac catgtggatg
     3421 cccgagatcg ccaaggcgga ggacgccggc gaccacttca cctggcactc ctggcactgc
     3481 agcggcatcg accgcaagat catcaccaag ggcatgggct acttcagtcc catgcgctac
     3541 tccgagctgc cccgcttcta ccgggagaat ctggcccctg tggacgtggt gatgctccag
     3601 accaccccca tggatgccca cggcaacttc aacttcggcc tggcggcctc ccacctggcc
     3661 gacctgatgt cccgtgccaa gtgcattatc gtggaggtca acgagaatat gccctgggtg
     3721 tacggcctca ccggcaccga gatcaacatc caggacgtga cctatgtcgt ggagggggac
     3781 aacccccccg tggctcagct gggcgcaggc ggcgagccta ccgacgtgga ccgggccgtg
     3841 gccaacctgg tggtgcccca gattcccaac ggggcctgcc tccagctggg catcggcggc
     3901 atgcccaaca ccatcggcgc catgatcgcc cagtccgacc tgaaggacct gtctgtccac
     3961 acggagatgt atgtggatgg cttcgtggac atggcggccg ccggcaagat caccggccgg
     4021 cacaaggccc ttgacaaggg ccgccaggtc ttcgccttcg ccgccggcac ccagaagctc
     4081 tatgactacg tcgaccgcaa ccccgacgtg atgggtgccc cggtagacta caccaacgat
     4141 gtgcgggtca tcgctcagat cgacaacttc atctccatca acaacgccat cgacatagac
     4201 ctgttcggcc agatcaacgc cgagtccgcc ggcatcaagc acatctccgg caccggcggc
     4261 cagctggact tcgccatggg cgcctacctg tccaacggcg gcaaaagctt catctgcatg
     4321 tcctccaccg tcaccggcaa ggacggtact gtcaagagcc gcatcgtccc caccctgacc
     4381 cagggctcca tctgcaccga cccccgctcc tgcggccact atgtggtcac ggagtacggc
     4441 a
//
LOCUS       DBMDSJ010000095         4372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5360_length_4372_cov_1.689066, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000095 DBMDSJ010000000
VERSION     DBMDSJ010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5360_length_4372_cov_1.689066"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="ACZPAQ_03410"
     CDS             complement(<1..935)
                     /locus_tag="ACZPAQ_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR0903061.1"
                     /translation="MDSTSIGMTVALVILVAMSAYFSATETAFTSLNRIRLKSRADNG
                     DQRAARALALAEDYDKLLSTILIGNNIVNNVATTIGAVLFIRLLDDVRGPTVSAIVLT
                     VVILIFGEVSPKSLAKESPEAWAMLSAPLLRAFVAVLTPANFLFTQWKRLLSLIIHKR
                     EEDGITEEELVTMVDQAENEGGLDEHESELIRNAIEFHDLEVSEILTPRVDIVAAEEN
                     STMEEVGSLFAESGYSRLPIYQDSIDNIVGVIHEKDFYAARYRGETMISALKAPVLYT
                     TGNTKISELMRILQRNKAHMAVVVDEYGGTEGIATL"
     gene            1169..1606
                     /locus_tag="ACZPAQ_03415"
     CDS             1169..1606
                     /locus_tag="ACZPAQ_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR0903062.1"
                     /translation="MKVRVEYAPVLENEVVLRCPRLDQEMLRVLSLVRSGLQKLCVWD
                     ENREIILLSPDETLYCESVDEHTFLYTASAFYQTALTLAELEGRYGELGYLRVSKSAV
                     INLHRIRSLKSRTGGRIEASLENGERLVVSRHYAPLLRERLGL"
     gene            1621..2076
                     /locus_tag="ACZPAQ_03420"
     CDS             1621..2076
                     /locus_tag="ACZPAQ_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HIR0903063.1"
                     /translation="MGEFIRFYSWGLSMKFHMAVYTLALVFFKAIFNALAGEGSVSSL
                     TMLQMLLVSMAVAILESFLFPEGRELEPSALRVRTVWWGLLCNLGFVGGALALGWFSN
                     VPLWGGIFLVLFLEWGLSAMWFGLHVALRRDTRSLNRKLSEYQKRGPES"
     gene            complement(2141..2572)
                     /locus_tag="ACZPAQ_03425"
     CDS             complement(2141..2572)
                     /locus_tag="ACZPAQ_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR0903064.1"
                     /translation="MSRMAVIYWSMTGNTEAMANAIAEGAAGAGAEVDVKQVCDTTVD
                     EALGYGLLALGCPAMGAEVLEEAEFEPFFTELEGRLGCKKVALFGSYDWGDGQWMRDW
                     AQRADSAGANVYQDEGLILHLTPDEEGLEQCRTFGAGFAKY"
     gene            complement(2674..3258)
                     /locus_tag="ACZPAQ_03430"
     CDS             complement(2674..3258)
                     /locus_tag="ACZPAQ_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HIR0903065.1"
                     /translation="MLTSEVRPAEARFELTLAFHCAPALAGIKPADLIALPGEGDETD
                     ALLASYSAALAGAGISLRPLCRCRSRCLVLVYRRNRLERQLEQEEVARLLEQDGYPLA
                     GGLEAMLDHLASRMAGSGGFPHEVGLFLGYPAEDVEGFRRHGGQGYKYSGLWKVYSDV
                     ERAQRCFRQYGCCRRALCRRLEAGCRLVQLFRTA"
     gene            complement(3446..4252)
                     /locus_tag="ACZPAQ_03435"
     CDS             complement(3446..4252)
                     /locus_tag="ACZPAQ_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024197.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HIR0903066.1"
                     /translation="MPQKKAVTPWEKEFARLERRRRAYQARHARPPESRLDRALEDKV
                     PPKLREALNSAFVKAFDLVFQRGTGLLERTFDAEAARQQFEIDQFAVQLKGDRSSLRV
                     FSRRAGRTGALNLALSGAEGTLLGLLGIGLPDIPLFTGVLLKSLYQMAARYGYGWDTP
                     AERYYLLLLVQGALSYGEELAACTALLDEFALTPCPPEDYDQGTQIRRTASLLADRLL
                     VMKFVQGIPILGMAGGISDALCLERVQRYAGLNYHRRFLLDHRPDPRPFA"
BASE COUNT          806 a         1384 c         1331 g          851 t
ORIGIN      
        1 agagtggcaa tgccctccgt gcccccgtac tcatccacca ccacggccat gtgggccttg
       61 ttccgctgga ggatgcgcat cagctcggaa atcttggtgt tgccggtggt gtagagcacc
      121 ggggccttca gggcggagat catggtctcc ccccggtagc gggcggcgta gaagtccttc
      181 tcatggatca cgcccacgat attgtcgatg ctgtcctgat agatgggcag gcgggagtaa
      241 ccgctctccg caaagaggga gcccacctcc tccatggtgc tgttctcctc cgccgccacg
      301 atatccaccc ggggagtgag gatctcggac acctccaggt cgtggaactc gatggcgttg
      361 cggatcagct cgctctcgtg ctcgtccagg ccgccctcgt tctccgcctg gtccaccata
      421 gtgaccagct cctcctcggt gatgccgtcc tcctcccgct tgtggatgat cagggagagc
      481 aggcgcttcc actgggtgaa caggaaattg gccggggtca gcacggctac aaaggcccgc
      541 agcagggggg cagagagcat ggcccaggcc tccgggctct ccttggccag gctcttgggc
      601 gagacctcgc caaagatcag gatcaccaca gtgagcacga tggcggacac cgtgggcccc
      661 cgcacgtcat ccagcagccg gatgaacagc accgcgccga tggtggtggc cacgttgttg
      721 acgatgttgt ttccgatgag gatggtggac agcagcttgt catagtcctc cgccagagcc
      781 agggccctgg cagcccgctg gtccccgttg tccgcccggc tcttcaggcg gatccggttc
      841 agggaggtaa aggcggtctc tgtggcggag aagtaagcgg acatggccac gaggatgaca
      901 agagcaacgg tcattccaat actggtactg tccatggggg acaaatcaac tcagctttct
      961 gtcataaatt cccggtcttc cgggtgctta gcacaggagt atatcacaga tattaaaaaa
     1021 atgcaaatct tataccggac aaattccgct gtatcccggc aaaatttggc ctggaggcag
     1081 gctgccggcc ccaaatggca tcctggcggc tcctggcttg ctctggcgtc ccggttctgg
     1141 tatgctcaca gagaagggag gtttttctat gaaagtgagg gtggaatacg cacccgtgct
     1201 ggaaaacgag gtggtcctgc gctgcccccg gctggatcag gagatgctcc gtgtcctgtc
     1261 tctggtgcgc tccggccttc agaagctgtg cgtatgggat gaaaaccggg agatcatcct
     1321 gctcagcccg gatgagacgc tctactgtga gagtgtggac gaacatacct ttctctatac
     1381 cgcctctgcc ttttatcaga cggccctcac cctggcggag ctggaggggc ggtatggaga
     1441 gctgggctat ctccgggtca gcaagtccgc tgttatcaac ctgcaccgca tccgcagcct
     1501 gaagagccgg actggcggcc gcatcgaggc ctccctggag aacggggagc ggctggtggt
     1561 ctcccggcac tacgcgcccc tgctgcggga gcggctggga ctataaaaag gaggtccatc
     1621 atgggagaat ttatccgttt ctacagctgg ggcctgtcca tgaaatttca tatggctgtc
     1681 tataccctgg cgctggtctt tttcaaggcc atcttcaacg ccctggccgg cgagggctcc
     1741 gtatcctcgc tcaccatgct ccagatgctg ctggtctcca tggcggtggc cattctggag
     1801 tccttccttt tcccggaggg ccgggagctg gagccgtccg ccctgcgggt gcggaccgtc
     1861 tggtgggggc tgctgtgcaa cctggggttt gtgggcggcg cgctggccct gggctggttt
     1921 tccaatgtgc cgctttgggg aggcatcttc ctggtgctct tcctggagtg gggcttgtcc
     1981 gccatgtggt tcggcctcca tgtggccctg cggcgggaca cccgctccct gaaccggaag
     2041 ctgagcgagt accagaagcg gggaccggag agttagagaa aaaagaggac ggataccctc
     2101 ttggggagac atccgtcctc tttttcttga tccggtccct ttaatacttg gcaaagcccg
     2161 ccccgaaggt gcggcactgc tccagcccct cctcatcggg ggtcaggtgg aggatcaggc
     2221 cctcgtcctg gtacacattg gctccggcac tgtccgcccg ctgggcccag tcccgcatcc
     2281 actgaccgtc accccagtca taggagccga acagagccac ctttttgcag cccagacggc
     2341 cctccagttc ggtgaagaag ggttcaaatt ccgcctcctc caacacctcc gcccccatgg
     2401 cggggcagcc cagtgccagc agtccatagc ccagcgcctc gtccaccgtg gtgtcgcaca
     2461 cctgcttcac atccacttca gctccagccc cggcggcccc ctccgcgatg gcgttggcca
     2521 tcgcctccgt gttgcccgtc atggaccagt aaatcactgc catcctgctc atgtatgtga
     2581 cctcctgaaa ataaaaattt ggtttgtata gcaaacgggg gggcgggccc atccgggcgc
     2641 cgcggatctc cccgcctcta tcccttatct ccgtcaagct gtccgaaaca gctggaccag
     2701 ccggcagccg gcctccaggc ggcggcacag ggcccttcgg cagcagccgt actgccggaa
     2761 gcatctctgg gccctctcca cgtcggaata gaccttccac agcccgctgt atttatagcc
     2821 ctggccgcca tgccggcgaa agccctccac atcctcggcg ggatatccca ggaacagccc
     2881 cacctcatgg gggaacccac cgctccccgc catccgggac gccagatggt ccagcatggc
     2941 ctccagcccg ccggccaggg gatagccgtc ctgttccagc agacgggcca cctcctcctg
     3001 ctccagctgg cgctccaggc ggttccgccg gtacaccagt accagacagc ggctgcggca
     3061 ccggcacagg ggccgcagag agatccccgc cccggccagg gcggcggagt aggacgccag
     3121 cagggcgtcg gtctcatctc cttcccccgg gagggcaatg agatccgcag gcttgatgcc
     3181 tgccagggcc ggcgcacagt gaaaggccag ggtcagctca aagcgcgctt cggccggccg
     3241 gacctcagaa gttagcataa gctaatccct cctttgaaaa atcgcgcctt tcggcgtttg
     3301 gctgtaaaag tttagtccct tttgttagcc ggtgctaacc aataaaaatc taccacagtt
     3361 tttccttcct gtcaaccact gattttgccc gctcccgttt tggcgggggc gggcacctgg
     3421 gatgcgggag ggacatgacc ctgctctatg cgaaaggccg gggatcgggc cggtggtcca
     3481 ggagaaagcg gcgatgatag tttaacccgg catagcgctg gaccctttcc agacagaggg
     3541 cgtcagagat gcccccagcc atgcctagga tggggatgcc ctggacaaat ttcatcacca
     3601 gcaggcggtc ggctaggaga gaggcagtcc ggcggatctg tgtcccctgg tcatagtcct
     3661 ccggcggaca gggggtgagg gcaaactcgt ccagcagcgc ggtgcatgcg gccagctcct
     3721 ccccatagga cagggcgccc tggaccagaa ggagcagata gtaccgctct gccggggtat
     3781 cccacccgta gccgtaacgg gcagccattt ggtagaggct tttcagcagg acgccggtaa
     3841 agagcgggat atcgggcagg ccgatcccca gcagccccag gagagtgccc tcggccccgg
     3901 aaagggccaa attgagggcc ccggtccgtc ccgctcggcg ggaaaagacc cgcagagagc
     3961 tccggtcccc cttcagctgg acggcaaact ggtcgatctc aaactgctgc cgggccgcct
     4021 cggcatcaaa ggtgcgctcc agaagcccgg tcccccgctg gaagaccaga tcaaatgcct
     4081 tgacaaaggc ggagttaagg gcctcccgga gtttgggcgg caccttgtcc tccagcgccc
     4141 ggtccagcct ggactccggc ggacgggcat ggcgggcctg ataggctctc ctgcggcgct
     4201 ccagccgggc aaattccttt tcccaaggtg tgacagcttt cttctgcggc acggctctcc
     4261 ctcctctgtg cataatggat gaggtgagga tatcacacag ccggggcgcc tgtcctgaaa
     4321 ttgggattaa aacttgtcac ttccccggcc cgaatgacga aattttgtaa aa
//
LOCUS       DBMDSJ010000096         4340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5398_length_4340_cov_1.519934, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000096 DBMDSJ010000000
VERSION     DBMDSJ010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5398_length_4340_cov_1.519934"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="ACZPAQ_03440"
     CDS             <1..507
                     /locus_tag="ACZPAQ_03440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0903067.1"
                     /translation="LEDAYTLLTGDRDPEHRAAFRPLFLEASMERQRRETTLFPGAPE
                     LLRELKARGIRLGIVSTKRSDTLEAILENRGVLGELDLIIGSDRVKRHKPDPEGLLAA
                     MSRLGAGPEETLFCGDTVLDAGAAQNAGTEFAAVLNGTTPASAFREWPHVHIAPDLWD
                     LKRWLEEQ"
     gene            639..983
                     /locus_tag="ACZPAQ_03445"
     CDS             639..983
                     /locus_tag="ACZPAQ_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR0903068.1"
                     /translation="MKFDKGLIAGSSTLLVLTLLESGDMYGYQMIEELSRRSNDAFQF
                     QEGTLYPILHGLEKGKYLSGYQQQAPSGRMRKYYRLTRKGRELLAEKKAEWASFHRGV
                     ADVLSGGANALA"
     gene            1013..1216
                     /locus_tag="ACZPAQ_03450"
     CDS             1013..1216
                     /locus_tag="ACZPAQ_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903069.1"
                     /translation="MTGEKRLALNLLILAALAALCVLAAQYPVLRLEWEARCAVEEEH
                     PGRAERVWRGRVSGSGGSGRHGA"
     gene            1418..1630
                     /locus_tag="ACZPAQ_03455"
     CDS             1418..1630
                     /locus_tag="ACZPAQ_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903070.1"
                     /translation="MRTADSVREGEVMVFCFREAELNWRQELTYQRAETSWKAALASA
                     PYTLTLWDRNGVLISETKGTMGSIWR"
     gene            1638..1988
                     /locus_tag="ACZPAQ_03460"
     CDS             1638..1988
                     /locus_tag="ACZPAQ_03460"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR0903071.1"
                     /translation="MKRSWGTECAPAWRAWLDRATEQIFRHPDCGAVERELLDHLEDK
                     ATDLMRIFPGLTREEAEQRALEEMGAAEELGEELARVHSPLVGELWQLTRFLIRAVLV
                     VLAVECLWLLWKLA"
     gene            1964..2170
                     /locus_tag="ACZPAQ_03465"
     CDS             1964..2170
                     /locus_tag="ACZPAQ_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903072.1"
                     /translation="MAALETCLTERARETEKAVKRCRGGKSRRTSWGVPCTLRVYGPG
                     LDLLEVEEIQVSTALSEEELARRQ"
     gene            2186..3028
                     /locus_tag="ACZPAQ_03470"
     CDS             2186..3028
                     /locus_tag="ACZPAQ_03470"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HIR0903073.1"
                     /translation="MVQTSEGRRWLDRAAAGIRSRPDREAVRAELEAHLGDKTADLVR
                     IFPDLERQEAERQAVEQMGDAEEIGKELARLHRPWPRYLWQASRVLLTAGLLWLLAIG
                     MFRGDDAYLGDDHRSEWWDMDGLPGTAVMGDCDDIRYLPGGDPDQILTLEPCLTASVN
                     GQKISLLRAALWQREDGRQGLYCYLRVDTWRFWERGRLREEWMTVTDSTGAVCGLGPD
                     SPEDPETGGLLNGMSQMGFGPFHSGYELYLEDWDPEAEWVRLDYGPGSPIFTFTVDLE
                     EERA"
     gene            3025..3726
                     /locus_tag="ACZPAQ_03475"
     CDS             3025..3726
                     /locus_tag="ACZPAQ_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903074.1"
                     /translation="MKGAWRALWERRPRLGHKGRAARNLLLVLALAALVWGQYGCPMP
                     TAEMAFRQLEGQYLLPRSEIVYQSGFWNTGDIGSVESRDGTYLSVFDPFVVGVAQNRV
                     YSVTLDRPGRQVLGVFPLGEGPAPVPINGVIAWVLEPGRTRMNGCNLLFLQIPGETAR
                     TELDVDTALPGGERFLRRAQEGFCLEDGVWLFPLESPEGGWSGDWYEGAAYTLRLYRE
                     DGALLLGQSGTVRES"
     gene            4162..>4340
                     /gene="rpsJ"
                     /locus_tag="ACZPAQ_03480"
     CDS             4162..>4340
                     /gene="rpsJ"
                     /locus_tag="ACZPAQ_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR0903075.1"
                     /translation="MAAQKEMIRIRLKAYDHQLIDQSAEKIVETAKRTGATVSGPIPL
                     PTKKEVVTILRAVHK"
BASE COUNT          768 a         1177 c         1674 g          721 t
ORIGIN      
        1 ctggaggatg cctacaccct gcttacaggg gatcgggacc cggagcaccg ggcggccttc
       61 cgtcccctgt tcctggaggc gtccatggag cgccagcgcc gggagaccac tctgtttcct
      121 ggtgccccgg agctgctccg ggagctgaag gcccggggga tcaggctggg gatcgtgagc
      181 accaagcgca gtgataccct ggaggccatc ctggagaacc ggggggtgct gggggagctg
      241 gacctgatca tcggcagcga ccgggtgaag cggcataagc cggacccgga ggggctgctg
      301 gctgccatgt cccgactggg ggcggggccg gaggagactt tgttctgtgg agacacggtg
      361 ctggatgcgg gggcggccca aaatgcgggg acagagttcg ccgcggtgct caatggcacc
      421 acgccggcct ctgccttccg ggagtggccc catgtccaca tcgccccgga cctttgggac
      481 ctgaagcggt ggctggaaga acaataaaaa ggcggcaggc catgcctgcc gccttttttc
      541 tgccccgtcc ctcttgacat acctagaata tctttgtata ttgagcctag aggtactagg
      601 tatatcgtcc ggcaggggcc ggagaaagga cggcgaacat gaaattcgat aaagggctga
      661 tcgcgggcag ctctacgctg ctggtgctga ctctgctgga gagcggggac atgtacggct
      721 atcagatgat cgaggagctc tcccgccgct ccaacgacgc ctttcagttc caagagggga
      781 ccctgtaccc cattctccac gggctggaga aggggaagta cctgagcggc tatcagcagc
      841 aggccccctc cggccggatg cggaagtact accggctcac ccggaagggg cgggagctgc
      901 tggcggagaa gaaggcggag tgggccagct tccaccgtgg cgtggccgac gtgctctccg
      961 gcggagccaa cgctctggcg tgaaggggat gagaggccgg aggcgcgccg gtatgaccgg
     1021 cgaaaagcgg ctggcgctga acctgctgat cctggcggcg ctggctgccc tgtgtgtgct
     1081 ggcggctcag tatcctgtgt tgcgcctgga gtgggaggcc cgctgcgctg tggaggaaga
     1141 gcaccccggc agggcggagc gggtctggcg cggcagggtg tccgggagcg ggggatcagg
     1201 ccgtcatggt gcgtagcggc gcagccgtgg gactgagcgt gctgacctgg tatgagggct
     1261 cccctagttt tttcatctgg cgggggcaga ttccggaggg gcttacggtg acagcggtcc
     1321 ccgggggtag cgggccgcct ttggagtata tggccctgga gctgccggag ggggctgtat
     1381 ccgggcggct gaccgcagag ctccttggag gagggatgtg agaacggcgg acagcgtccg
     1441 ggagggggaa gtcatggtgt tctgtttccg ggaggcggag ctgaactggc ggcaggagct
     1501 gacctaccag cgggccgaga cctcctggaa ggcggccctg gcgagtgcgc cttatacgct
     1561 gactctttgg gaccggaacg gggtcctgat ttctgagacc aaagggacca tggggagcat
     1621 ctggaggtga gggcggcgtg aaacgatcgt ggggaacgga gtgcgcaccg gcttggcgtg
     1681 catggctgga ccgggctacg gagcagatct ttcgtcaccc ggactgcggc gcggtggagc
     1741 gggagctgtt ggaccatctg gaggacaagg ccaccgacct gatgcgtatt tttcccggcc
     1801 tgactcggga ggaggcagag cagcgggccc tggaggagat gggggcggcg gaagagctgg
     1861 gggaggagct ggcccgcgtc cacagcccgc tggtgggaga gctgtggcag ctgacccgct
     1921 tcctgatccg ggcggtgctg gtggtgctgg cggtggagtg cctgtggctg ctctggaaac
     1981 ttgcctgaca gagcgggcca gggagacgga aaaggcggtg aagagatgca ggggcggaaa
     2041 aagcagaaga acgagctggg gcgtcccctg caccctacgg gtgtacggcc ctgggctgga
     2101 cctgctggag gtggaggaga tccaggtcag cacggcgctg agtgaagagg aactggcgcg
     2161 gagacagtga gcggggagga acgagatggt acagacatca gagggaaggc gctggctgga
     2221 ccgggcggcg gcaggtatcc gctcccggcc tgaccgggag gcggtgcggg ccgagctgga
     2281 ggcccacctg ggggataaga cggcggacct ggtccgcatc ttcccggatt tggagcggca
     2341 ggaggcggag cggcaggcgg tggagcagat gggggacgca gaggagatcg gaaaggagct
     2401 ggcccggctc caccggccct ggccgaggta cttgtggcag gccagccggg tgctgctcac
     2461 ggcgggcctc ctgtggctgc tggccatcgg tatgttccgg ggggatgacg cctatctggg
     2521 ggacgaccac cgctccgagt ggtgggacat ggacggcctg cctgggacgg cggtcatggg
     2581 ggactgcgac gatatccgct atctgcccgg tggggacccg gaccagatcc tcaccctgga
     2641 gccctgcctg acagcatcag tgaatggaca gaagatctca ctgctccggg ccgccctgtg
     2701 gcagcgggag gacgggcggc aggggctgta ctgctatctc cgggtggata cctggcgctt
     2761 ctgggagcgg ggccggctgc gggaggaatg gatgactgtg accgacagca ccggcgcagt
     2821 ctgcggcctg gggccggatt ccccggagga cccggaaacg ggcggactgc tgaacgggat
     2881 gagccaaatg ggcttcgggc ccttccacag cggctatgaa ctgtatctgg aggactggga
     2941 cccggaggcg gagtgggtcc ggctggacta cggcccaggg agcccgatat tcacttttac
     3001 tgtggacttg gaggaggaga gggcgtgaag ggagcgtgga gggcgctgtg ggagcgccgc
     3061 ccccggctgg ggcacaaggg aagggcggcc cggaacctgt tgctggtcct ggcgctggcc
     3121 gccctggtgt gggggcagta cggctgtccc atgcccacag cggagatggc gttccggcag
     3181 ctggaggggc agtatctgct gccccggagc gagatcgtat atcagagcgg cttttggaat
     3241 accggagata tagggagtgt ggagagccgg gatgggacct atctgagtgt gttcgatccg
     3301 tttgtggtgg gtgttgccca gaatcgggtc tattctgtca ccttggaccg gcccgggcgg
     3361 caggtcctgg gggtctttcc cctgggggag gggccggctc cggtcccgat caacggtgtg
     3421 attgcctggg tcctggagcc cggaaggacg cggatgaacg gctgcaacct cctgtttctc
     3481 cagatcccag gggagacggc ccggacggag ctggatgtgg acaccgcgct tccggggggt
     3541 gagcgcttcc tccgccgcgc ccaggagggc ttctgtctgg aggacggcgt gtggctgttt
     3601 ccgctggaat caccggaggg agggtggtcc ggggactggt acgagggggc ggcgtacacc
     3661 ctccgcctgt accgggagga cggggcgctg ctcctggggc agagcggcac ggtgcgggag
     3721 tcctgagcgc aggcggggcc ccggggaccg tgtttcgggg gcccgccggc gggagcggcg
     3781 ggccgccctg gcaggatgcc caaagaggcc ggaggaaaaa gccggggaaa ttggcggaac
     3841 accgccgaaa aattttcaaa acacccttga cacacacggg ggtgttgttg tatgatagca
     3901 aagcgcctgt gagggcgcag tctgcgatga tgcgggagat tgctcggaaa cgaggtaact
     3961 tccgcggagt atgtccgaga atcgggcggc tgagaggatc cgtgtcacgg cgtatatcgc
     4021 ctggcaggga taaaaccggc ctgtctatgc gccggttttc ttcatgaccc ggagtgatac
     4081 gcacgatagc gtggacggag atctctcacc gtacgaagcg cgacagagtc aacttcgtat
     4141 gtatgttaag gaggaacacc catggcagct cagaaagaaa tgatccgcat ccgtctcaag
     4201 gcctatgatc atcagctcat cgaccagagc gctgagaaga tcgtggagac cgccaagcgc
     4261 accggcgcca ctgtctccgg ccccatcccc ctgcccacca agaaggaagt cgtcaccatc
     4321 ctgcgggccg tccacaagta
//
LOCUS       DBMDSJ010000097         4333 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5410_length_4333_cov_1.680529, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000097 DBMDSJ010000000
VERSION     DBMDSJ010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_4333_cov_1.680529"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="ACZPAQ_03485"
     CDS             complement(<1..387)
                     /locus_tag="ACZPAQ_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0903076.1"
                     /translation="MIYFLEDDPSIRKLVIYTLNSQGMEAEGFELPSQFWAAMERQLP
                     SLVLLDLMLPEEDGLHILKRIRAGASTARLPVILITAKGTEYDKVVGLDGGADDYIAK
                     PFGMMELMARIRTALRHSGGEAEPVHA"
     gene            complement(431..1075)
                     /gene="phoU"
                     /locus_tag="ACZPAQ_03490"
     CDS             complement(431..1075)
                     /gene="phoU"
                     /locus_tag="ACZPAQ_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR0903077.1"
                     /translation="MRVKFEAQLERLNVELITMGALCEEVISGAAKALLDGDSGLRET
                     VLAAERDINQKERDIESLCMKLLLQQQPVARDLRTISSALKMISDMERIGDQAADIAE
                     ITRDIADNSIKDLVPIGDMARATIKMVTDSVDSFVRKDLGLAEAVISSDDVVDDLFLQ
                     VREELIRRIGMGDSGEVCIDLLMIAKYLERIGDHAVNIAEWVEYSLTGHHADLP"
     gene            complement(1075..1836)
                     /gene="pstB"
                     /locus_tag="ACZPAQ_03495"
     CDS             complement(1075..1836)
                     /gene="pstB"
                     /locus_tag="ACZPAQ_03495"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR0903078.1"
                     /translation="MDQSVILSACGLNLYYGENHALKDISIDIKANKITALIGPSGCG
                     KSTFLRTIDRMNDLIPSVKITGSLTFHGQEIYAPGVDVTDLRRRIGMVFQKPNPFPMS
                     IYDNVAYGPRTHGVRSKLALDELVEKSLRQAAIWDEVKDRLKKPALGLSGGQQQRLCI
                     ARALAVQPEVLLMDEATSALDPISTSKIEDLAMELKSEYTIVMVTHNMQQAARVSDQT
                     AFFLLGDLVEYGDTEQLFSMPQDKRTEDYITGRFG"
     gene            complement(1848..2807)
                     /gene="pstA"
                     /locus_tag="ACZPAQ_03500"
     CDS             complement(1848..2807)
                     /gene="pstA"
                     /locus_tag="ACZPAQ_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR0903079.1"
                     /translation="MTNAMISSEEAQSYQSKGLSPRRRTYEVGMRGLLYLSAGITCLL
                     LLFLIGYILYRGLPNLNWTFLTSQESVLRGTDGIMPAIQNTLYVIVVTLIFILPLGVG
                     AAIYLTEYASNHKLVSAIEFATETLAGIPSILYALVGMLIFCQVMDLGKTLLAGALTL
                     VIMSLPTIIRTTQESLKTVPQSYREGSLGLGAGKWHMIRTVVLPSSVDGIVTGCILSI
                     GRVVGESAVLMFTAGMGTTLQNFFGSFNDQALSHFLSTGDLSLLFSGLTDSSGATLTV
                     ALYVYAKERAEFDLAFSVALVLLAITLAINLSAKLVGKRLKKR"
     gene            complement(2811..3668)
                     /gene="pstC"
                     /locus_tag="ACZPAQ_03505"
     CDS             complement(2811..3668)
                     /gene="pstC"
                     /locus_tag="ACZPAQ_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR0903080.1"
                     /translation="MELIMMIVFLVCGFIAIAFVLLITVYLIISGLPAIREVGLTDFL
                     FGTRWASTDKTDPAYGILPFILTSIYGTAGAILIGVPIGFLTAVFLSKVAPPRVAAVI
                     RPAVDLLAGIPSVVYGLVGMMVLLPAIRKTFDIAAGDSLLAAIIVLAVMILPSIISVS
                     ETALNAVPREYEEASLALGATELETYFRVSVPAAKSGIAAAVVLGVGRAIGEAMAILM
                     VAGNVANMPSLLSSVKFLTTAIASEMSYAAVGSLQRNALYSIGLILFLFIMCINVILN
                     VFLKRNKEG"
     gene            complement(3884..>4333)
                     /locus_tag="ACZPAQ_03510"
     CDS             complement(3884..>4333)
                     /locus_tag="ACZPAQ_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0903081.1"
                     /translation="QIAQIFTGEITNWSELGGADAEIAVFGREAGSGTRGAFEEIVGV
                     EDACKYTNEYSSTGDVIGNVASNPNGIGYASLSAVDDTVKAVAVNGVTPSEDTVKDGS
                     YEIQRPFVMVTKEGTQLSEAAQAFLDFAMSADAAEIIAVAGAVSANA"
BASE COUNT          832 a         1244 c         1475 g          782 t
ORIGIN      
        1 ggcgtggacc ggctccgcct ccccgccgct gtggcgcagg gcggtgcgga tgcgggccat
       61 cagctccatc atgccgaagg gcttggcgat gtagtcgtcc gccccgccgt ccaggcccac
      121 caccttgtcg tactcggtcc ccttggcggt gatcaggatg acggggagcc tggcggtgga
      181 ggcgccggcg cggatgcgct tgaggatgtg caggccgtcc tcctccggga gcatcaggtc
      241 cagcaggacc agggagggga gctgccgctc catggcggcc cagaactggg agggcagttc
      301 aaagccctcc gcctccatgc cctggctgtt cagggtataa atgaccagct tgcggatgct
      361 ggggtcgtct tccagaaaat aaatcatggg taggacctcc tacggctgtg aaaattgggg
      421 cgcccgccgt tcagggcagg tcagcatggt gcccggtgag ggagtactcc acccactcgg
      481 cgatgttgac ggcgtgatct ccgatgcgct ccagatattt ggcgatcatc agcaggtcga
      541 tgcacacctc tccgctgtcc cccatgccga tgcggcggat cagctcctca cggacctgga
      601 gaaacagatc gtccaccacg tcgtcggagg agatcactgc ctccgccaga cccaggtcct
      661 tgcggacaaa ggagtccacg ctgtcggtga ccatcttgat ggtggcccgg gccatgtccc
      721 cgatagggac caggtccttg atgctgttgt cggcgatatc ccgggtgatc tcagcgatgt
      781 ctgcggcttg gtcgccgatg cgctccatgt cagagatcat cttcagggcg gaggagatgg
      841 tgcgcagatc ccgggccacc ggctgctgct ggagcagcag cttcatacac aggctttcga
      901 tgtcccgctc cttttggttg atgtcccgct ccgcggccag cacggtctcc cgcaggccgg
      961 aatcgccgtc cagcagggcc ttggccgcgc cggagatgac ctcctcgcac agggcaccca
     1021 tggtgataag ttctacgttg agccgctcca gctgtgcttc aaatttgact cgcattatcc
     1081 aaacctcccg gtgatgtagt cctcggtgcg cttgtcctgg ggcatggaaa agagctgctc
     1141 cgtgtccccg tactccacca ggtcgcccag caggaagaaa gcggtctggt cggagacgcg
     1201 ggcggcctgc tgcatgttgt gagtcaccat gacgatagtg tactcgcttt tcagctccat
     1261 agcaaggtct tctattttgg atgtagagat ggggtccagg gcggaggtgg cctcgtccat
     1321 cagcaggacc tccggttgga cggccagggc ccgggcgatg cacaggcgct gctgctggcc
     1381 gccagacagg cccagggcgg gctttttcag ccggtctttc acctcgtccc agatggcggc
     1441 ctgacgcagg gacttctcca ccagctcgtc caaggccagc ttggagcgca ctccatgggt
     1501 gcgggggccg taggccacgt tgtcgtagat agacatggga aaggggttgg gcttctggaa
     1561 caccatgccg atgcgccggc gcaggtcggt gacgtccacg ccgggggcgt agatctcctg
     1621 cccgtggaag gtgagggaac cggtgatctt cacgctgggg atcaggtcgt tcatccggtc
     1681 gatggtccgc agaaaggtgg acttgccgca gccggagggc ccgatgaggg cggtgatctt
     1741 gttggctttg atgtcgatgg agatgtcctt cagtgcgtgg ttctcaccgt aatacaggtt
     1801 cagcccgcag gccgagagaa tgacagattg atccatagtt gtcctcctta gcgctttttc
     1861 aggcgcttgc ccaccagctt ggcggacaga ttgatggcga gggtgatggc cagcaggacc
     1921 agggccacag agaaggccag gtcgaactcg gcccgctcct tggcgtagac gtacagggcc
     1981 acagtgaggg tggcaccgga gctgtcagtc aggccgctga acagaaggga aaggtcaccg
     2041 gtggacagaa aatgcgacag agcttggtcg ttgaagctgc cgaaaaagtt ctggagggtg
     2101 gtgcccatgc cggcggtgaa catgagcacc gcagactcgc ccaccacacg gccgatggac
     2161 aggatgcagc cggtgacgat gccgtccacg gaggagggga gcaccacggt gcggatcatg
     2221 tgccacttgc cggcccccag gcccagggaa ccctcccggt agctctgggg caccgtcttc
     2281 agggactcct gggtggtgcg gatgatggtg ggcagggaca tgatgaccag ggtgagggcg
     2341 ccggccagca gggtcttgcc caggtccatg acctgacaga agatcagcat acccaccagg
     2401 gcgtacagga tagaggggat gccggccaga gtctcggtgg cgaactcgat ggcagagacc
     2461 agcttgtggt tggaggcgta ctcggtgagg tagatggcgg cccccacccc caggggcagg
     2521 atgaagatca gggtgaccac gatgacatag agggtgttct ggatggcagg catgatgccg
     2581 tcggtccccc gcaggacact ctcctggctg gtgaggaagg tccagttcag attgggcagg
     2641 ccccggtaga ggatgtagcc aatgagaaag aggagcagca ggcaggtgat gcccgcggag
     2701 agatacagca gcccgcgcat ccccacctca taggtgcggc ggcggggaga gaggcccttg
     2761 gactggtagc tctgggcctc ttccgaggag atcatggcgt tggtcatagc tcagccctcc
     2821 ttgttccgtt tcaggaatac gtttaggatc acattgatgc acatgataaa cagaaatagg
     2881 atcagcccaa tggagtacag ggcgttgcgc tggaggctgc ccacggcggc gtagctcatc
     2941 tcgctggcaa tggcggtggt gaggaatttg acgctggaga gcagagaggg catgttggcc
     3001 acattgccgg ccaccatcag gatagccatg gcctcaccga tggcccggcc cacgcccagc
     3061 accacagcgg cggcgatgcc gctcttggct gcggggacag agacccggaa gtaggtctcc
     3121 agctcggtgg cccccagggc caggctggcc tcctcatact cccggggcac cgcattcagg
     3181 gcggtctcgg acaccgagat gatggagggc aggatcatca ctgccaatac gatgatggcg
     3241 gccagcaggc tgtcaccggc ggcgatgtca aaggttttac ggatggcggg gagaaggacc
     3301 atcatgccca ccaggccgta gaccaccgag gggatgcccg ccagcagatc cactgccggg
     3361 cggatgacgg cggccaccct ggggggcgcc accttggaga gaaagacggc ggtgaggaag
     3421 ccgatgggca ccccgatgag gatggccccg gcggtgccat agatagaggt gaggataaag
     3481 gggaggatgc cataggcggg gtcggtcttg tcggtggagg cccagcgagt tccgaagagg
     3541 aagtcggtca accccacctc acggatagcg ggcaggccgg agatgatcag gtacacggtg
     3601 atcagcagga caaaggcgat ggcgatgaag ccgcagacca gaaacacaat catcataatc
     3661 agctccatgg cctctttggc ccggagggcg cccccgccgg ggacgccggg atgctctttt
     3721 ttcatagtac tcaacctttt cttgtagatt ctgtctcctc agctgacaca cgggcgcggg
     3781 ggccgtgcct gtgtgtcaac tgaggaaagg gggcagaagg ggagacatca ttcaaaacaa
     3841 gcccccgggc tcgggggctt gttttgaatt ggatcgggtg agctcaggcg ttggcagaca
     3901 cagcgccggc aacagcaatg atctcagcgg cgtcagcgct catggcaaaa tccaggaaag
     3961 cctgggctgc ctcggagagc tgggtgccct ccttggtgac cataacgaag ggacgctgga
     4021 tctcatagga gccgtccttc acggtgtcct cggagggggt cacgccgttg accgccacgg
     4081 ccttcacggt gtcgtccacg gcggacaggg aggcatagcc gatgccgttg gggttggagg
     4141 ccacgttgcc gatgacgtcg ccggtggagg aatactcgtt ggtgtactta caggcatctt
     4201 caacgcccac gatctcctca aaggcccccc gggtgccgga gccggcctcg cggccaaaga
     4261 ccgcgatctc ggcatcggcg ccgcccagct cggaccagtt ggtgatctcg ccggtgaaga
     4321 tctgggcgat ctg
//
LOCUS       DBMDSJ010000098         4313 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5439_length_4313_cov_1.328585, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000098 DBMDSJ010000000
VERSION     DBMDSJ010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5439_length_4313_cov_1.328585"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="ACZPAQ_03515"
     CDS             <1..603
                     /locus_tag="ACZPAQ_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903082.1"
                     /translation="ASQSAELFQRVEVTPQTVQSVIRTLERSTSYYWELTVETFWTGG
                     SSATSVQTWVDGGWSHTRQVLPSGLIRHDLISEDTVYYWYEGSSDYLTAPAEGGSADL
                     AQHIPTYETVLDADPEQITGTGYDDSRGGIPCIYVEVSAPGGERVDRYWISVESGLLI
                     SSETLEGEELLYRMTAYSPIQSCPSGTTFQLPDGTVLHSI"
     gene            complement(612..1076)
                     /locus_tag="ACZPAQ_03520"
     CDS             complement(612..1076)
                     /locus_tag="ACZPAQ_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903083.1"
                     /translation="MYNRYIPQNGTYTRVVEQDGPEEGARRPAPHPPGPERGENGPRG
                     SAGPPPGAARGAPGGGGRRPAPGPMGFGGLGDLLSGEKSPLNALFGGDKGGINGILKV
                     LRLEELDSGDILLILIILLLLVEGDNMDLVITLGLLLLLGLADDRKEEKQGG"
     gene            complement(1069..1521)
                     /locus_tag="ACZPAQ_03525"
     CDS             complement(1069..1521)
                     /locus_tag="ACZPAQ_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903084.1"
                     /translation="MGEMEDKLNAILNDPSAMGQIMALAQSLGGGAPRPQASPAGEAA
                     QGAEQTGAREAEAPASAGSAPPALAGGEDPLAALGEIDPGMIQMGMRLLREYQGEDDR
                     TVALLNALRPFLKESRLAKLDRAVQISRLARVIRVLFRGMGEGGRGDV"
     gene            complement(1558..1839)
                     /locus_tag="ACZPAQ_03530"
     CDS             complement(1558..1839)
                     /locus_tag="ACZPAQ_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903085.1"
                     /translation="MNQERSGKKGQESLPADLVKNKEALNRLARSGDAQRLMELLRQG
                     GGIQGAAEAAVKGDTSQLVGMMQRLMNTPEGGELVERISRQARQSGLTD"
     gene            1905..2363
                     /locus_tag="ACZPAQ_03535"
     CDS             1905..2363
                     /locus_tag="ACZPAQ_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0903086.1"
                     /translation="MKILVFSDSHRYTGGMIQAIEEQQPDLVVHLGDLQRDAEELALL
                     YPKLSMVTVPGNCDGWTTDPLERQFMVCGKRVLLSHGHIWHVKQGYDAALRAARNAGA
                     HLLLFGHTHRPCCFQEPDGLWVLNPGSARSTFGLVLPEGEALSCSLLDMP"
     gene            complement(2431..3009)
                     /locus_tag="ACZPAQ_03540"
     CDS             complement(2431..3009)
                     /locus_tag="ACZPAQ_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR0903087.1"
                     /translation="MSMTREQAWDLLTQYNQEEFHLRHAVTVEHVMRWFARELGYGDR
                     EEFWGIVGLLHDLDFERWPEEHCKKSQELMRQAGVEEEIVRATASHGWGHCVDIKPEQ
                     EMEKVLFAVDELTGLIGAAALMRPSKSVSDMELKSLKKKVKDKKFAAGCSRDVIAQGA
                     ELLGWELDQLLDRTLAAMRAEEAAIEEAAAQG"
     gene            complement(3126..3962)
                     /locus_tag="ACZPAQ_03545"
     CDS             complement(3126..3962)
                     /locus_tag="ACZPAQ_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR0903088.1"
                     /translation="MSFKIIVDSCCDLTGTMLKDPCFVKVPLTIRVENSSFVDDASLD
                     RSDLLWAMKQSEDAPSTACPAPQSYLDAYQCGADDIYVVTLSALLSGSHNSAVQARVL
                     LEEEEPERNVHVFNSCSASSGEVLTALKIRELASSGMPFKKVVREVEQYICQMQTLFV
                     LESLENLRKNGRLTKLQAVVTGALRIKLLMGATPQGEICKLGQALTMKQALGKLVDRM
                     AADPDHQGRVAAVCHCNCLERAFALKEQIEAKCQFQQVLILEAGGITTVYANDGGVVV
                     AY"
BASE COUNT          661 a         1597 c         1261 g          794 t
ORIGIN      
        1 gcctctcagt ccgccgagct gttccagcgg gtggaggtga ccccccagac cgtccagagc
       61 gtcatccgca ccctggagcg ctccaccagc tattactggg agctgacggt agagaccttc
      121 tggaccgggg gctccagtgc cacctccgtc cagacctggg tggacggagg ctggtcccat
      181 acccgtcagg tcctcccctc cggtctgatc cgccacgacc tcatcagcga ggacaccgtc
      241 tactactggt atgaggggtc ctccgactac ctcaccgccc cggcggaggg cggctccgcc
      301 gatctggccc agcacatccc cacctatgag accgtgctgg acgcggaccc ggagcagatc
      361 accggcaccg gctacgacga ctccaggggg ggcatcccct gcatctatgt ggaggtatcc
      421 gcccccggcg gggagcgggt ggaccgctac tggatcagcg tggagagcgg gctgctcatc
      481 tcctctgaga ctctggaggg ggaggagctg ctctaccgca tgaccgccta ttctcccatc
      541 cagtcctgcc cctccggcac cacctttcag ctgccggacg gaacggtgct ccacagcatt
      601 tgaggaggag ctcacccccc ttgcttctcc tccttcctgt catccgccag ccccagcagg
      661 agcagcagcc ccagggtgat gaccaggtcc atgttgtccc cctccaccag caggagcagg
      721 atgatcagga tcagcaggat atccccgctg tccagctcct ccagccggag gactttcagg
      781 atgccgttga tccccccctt gtccccgccg aacagggcgt tcagcgggct cttctcccct
      841 gagagcaggt cccccaggcc gccaaagccc atgggacccg gggcaggccg gcggcctcct
      901 cctccgggcg cgccccgggc tgccccgggt ggaggtcctg cggagccccg gggcccgttc
      961 tccccccgct ccggcccggg cggatggggc gccggacgcc gggccccctc ctctgggccg
     1021 tcctgctcca ccacccgggt ataagtacca ttctggggga tatagcggtt atacatcgcc
     1081 ccgcccccct tctcccatgc cgcggaagag gacccggatc acccgggcca gccgggagat
     1141 ctgcaccgcc cggtccagct tggccaggcg gctctccttc aggaaggggc gcagggcgtt
     1201 gagcagggcc accgttcggt cgtcctcccc ctgatactcc cgcagcagcc gcatccccat
     1261 ctggatcatg ccggggtcga tctcccccag ggcggccagc gggtcctctc cccccgccag
     1321 ggccggcgga gcgctccccg ccgaagcggg cgcctccgcc tccctggcgc cggtctgctc
     1381 cgccccctgg gcggcctccc ccgccgggga ggcctgcggc cggggagcgc cgccgcccag
     1441 ggactgggcc agcgccatga tctgccccat ggcggacggg tcattgagga tcgcgttcag
     1501 cttgtcttcc atctccccca cagggcagcc cctcctttcc ccctcctgtg cggggactca
     1561 gtcggtcagg cccgactgcc tggcctggcg gctgatgcgc tccaccagct cgcctccctc
     1621 cggggtgttc atcagccgct gcatcattcc caccagctgg ctggtgtccc ctttcacggc
     1681 ggcctccgcc gccccctgga taccgccccc ctgccgcagc agctccatca gccgctgggc
     1741 atcgccggat ctggccagac ggttgagggc ctccttattt ttgaccagat cggcgggcag
     1801 gctctcctgc ccctttttgc cgcttctttc ctgattcata ccggatctcc cttccaagag
     1861 gatttcattc cagtatatgc gccagacgag gtaaggtgac tcttttgaaa attcttgtgt
     1921 tttccgactc ccaccgctac accggcggca tgatccaggc catcgaggag cagcagcccg
     1981 acctggtggt ccacctgggc gacctccagc gggacgccga ggagctggcc ctcctctatc
     2041 ccaagctgtc catggtcacc gtgcccggca actgcgacgg ctggaccaca gatcctctgg
     2101 agcgccagtt catggtgtgc ggcaagcgcg tcctcctctc ccacgggcac atctggcatg
     2161 tgaagcaggg gtatgacgcc gccctccggg cggcccggaa cgccggggcc caccttctgc
     2221 tcttcggcca cacccaccgc ccctgctgct tccaggaacc ggacggcctc tgggtcctca
     2281 atccaggcag cgcccgcagc accttcggcc tcgtcctccc agagggggag gctctctcct
     2341 gttccctgct ggacatgccc tgatgggcgc ctctgtacaa aaaacacccc cgggacccgt
     2401 tcacggtccc gggggtgttt tttatatgtc tcagccctgg gcagcggcct cctcaatggc
     2461 cgcctcctcc gcccgcatgg ctgccagagt gcggtccagc agctggtcca gctcccagcc
     2521 cagcagctcc gccccctggg cgatcacgtc ccgggagcag cctgcggcga atttcttgtc
     2581 cttgactttc ttcttcaggg acttgagctc catgtcggac acgcttttgg aggggcgcat
     2641 cagggcggcc gccccgatca gcccggtgag ctcgtccacc gcgaagagga ccttctccat
     2701 ctcctgctcc ggcttgatgt ccacacagtg gccccagccg tggctggcgg tggcccggac
     2761 gatctcctcc tccaccccgg cctgccgcat cagctcctgg gactttttgc agtgctcctc
     2821 cggccagcgc tcaaaatcca ggtcgtggag cagacccacg atcccccaga actcctcccg
     2881 gtccccatag cccagctccc gggcgaacca gcgcatcacg tgctccactg taacggcgtg
     2941 gcgcaggtgg aactcctcct ggttatactg ggtcagcagg tcccaggcct gctctcttgt
     3001 catggacatc tctgaaacac tccttttttc acttctgaac tccttctgtg ctcctgggga
     3061 cgaagctccc ccggccctcc cccgccgggg acgggcatac agacggagcg gggcttttga
     3121 ctgttttaat aggccacgac cacgccgccg tcgttggcgt agacggtggt gatcccgccg
     3181 gcctccagga tcaggacctg ctggaactgg catttggcct cgatctgctc cttcagggcg
     3241 aaagcccgct ccaggcagtt gcagtggcac accgccgcca cccgcccctg atgatccggg
     3301 tccgccgcca tccggtccac cagcttgccc agagcctgct tcatggtcag ggcctggccc
     3361 agcttgcaga tctccccctg aggggtggcc cccatcagca gcttgatccg cagcgccccg
     3421 gtgaccaccg cctgcagctt ggtcagccgt ccgttcttcc gcagattctc cagggactcc
     3481 agcacgaaaa gggtctgcat ctggcagata tactgctcca cttcccggac caccttcttg
     3541 aagggcatcc cggaggacgc cagctcccgg atcttcagcg ccgtcagcac ctctccagag
     3601 gaggcggagc aggagttgaa cacgtggacg ttgcgctccg gttcctcctc ttccagcagg
     3661 acccgggcct ggaccgcgct gttgtgggag ccgctgagca gggcggacag ggtgaccaca
     3721 tagatgtcat ccgcgccgca ctggtaggcg tccagatagc tctggggggc ggggcaggcg
     3781 gtggaggggg cgtcctcgct ctgcttcatg gcccacagca ggtcgctccg gtccagggag
     3841 gcgtcgtcca cgaaggagct gttctccacc cggatggtca gaggcacctt cacaaagcag
     3901 gggtccttca acatggtccc ggtcaggtcg cagcagctgt ccacaatgat cttgaaactc
     3961 atacttttca tccccgaaca tgatattaga tattagattt acgatactat accacagttc
     4021 cgcccaaatg taaagtaaaa ccgcggcgcc tcccgtgtgc aggatcgccc cggagggcgc
     4081 agtcccccgc cgcttaccga ggcggacctc tgtaaccgcc caaaccctcc cctttaggga
     4141 gaaatgctgc ggcgtgggct gtccccctct cggccccgcc cccccaaagc cttcccccct
     4201 ccggggggaa ggtgtcacgg cgtgcgccgt gacggatgag gggtcttcct ttccccgtcc
     4261 cgctttttcg agaagggcgg ggcaagcccc gcccctacgg agtgactgga aac
//
LOCUS       DBMDSJ010000099         4263 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5503_length_4263_cov_1.669149, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000099 DBMDSJ010000000
VERSION     DBMDSJ010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5503_length_4263_cov_1.669149"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(178..903)
                     /locus_tag="ACZPAQ_03550"
     CDS             complement(178..903)
                     /locus_tag="ACZPAQ_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR0903089.1"
                     /translation="MDAETQRQTPKLRTNLDTNFLKLFAILTMVVDHVGTVFFPQHPE
                     FRWVGRLAFPIFCYCLTVGLMYTRDIRRYLGRLALFAVVSQPFFVLAFHPHNFWEELT
                     SWNIFVTLFFSLLAIYGVKERKWWWFLLGVLAINVLNADYANTGVILTLIFYLCRNKP
                     WLGALLYVLSYLPALWGGSLEDPMALVVGGHAIGFEIFALLSAPLIFLPTHVEPKIPK
                     WFFYAFYPAHLFIIFLVRTALHI"
     gene            complement(1314..3308)
                     /locus_tag="ACZPAQ_03555"
     CDS             complement(1314..3308)
                     /locus_tag="ACZPAQ_03555"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903090.1"
                     /translation="MSDVRKSVPAKVIASLLILVSVFSLCWQGMAVLRGIDYVLTDSW
                     QDTWEFKELIREREDQLAEWFAARAALEDPGLAYLDQVRYETDAERIGAAMAQDQTSF
                     RYRVLSQDGTKVLATNLAPGESLEQLVPEIHSAVFRPGEHLTQRWNSSPDAGDPALVT
                     EGSEESGDAYYIIECGVPQELSGAEDEFSELHARDTGGRKEIPLRIAATCVCLVLSLA
                     CFAFLMWSSGHKEGVEGIVLTWQEKIYFDLYLIGAGVLFFILIFPLMEFLYGWGYASQ
                     ESYENQVSATVVSLCVTAMTALAVLVLRTLVVRCKAGVLARTTLVCRACSWIWSGLLD
                     AIRSFPMIWRSVAGFLAYLVLCFFARRIWWDNPIPLFLINLGMLLWLCHWSVCFQRLR
                     KGSEAIAGGNLNHQIDTTRMPHDLRLHAEALNNIADGLNGAVEEKMKSERFKAELITN
                     VSHDLKTPLTSIINYVNLLKMEEVENPKAREYIEVLDRKSQRLKKLTEDLVEASKAST
                     GALTVNREKIGMCQLVDQALGEYEEKLEARKLTVVPSLPEGETYVYADGRHLWRVIDN
                     LLGNCAKYAMEGTRIYLELVRGKGQVTLSIKNISRDPLNVPPERLMERFVRGDESRTT
                     EGSGLGLSIARSLTELQGGTFELAVDGDLFKATVTMPQSG"
     gene            complement(3309..4004)
                     /locus_tag="ACZPAQ_03560"
     CDS             complement(3309..4004)
                     /locus_tag="ACZPAQ_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0903091.1"
                     /translation="MYTILICDDDRDIVSALDIYLTSEGYRTIAAYNGREAIQAAEEN
                     EIHLILMDVMMPELDGIRATAKLREGSNVPIILLTAKSEDTDKILGLNIGADDYITKP
                     FNPLEVIARVKSQLRRYTALGGAEKAPGMLTVGPISMDDGSKQVMVDGAPVSLTPIEY
                     NILKLLLSHPGQVFSSAQIYEQVWNDPAYGSENTVAVHIRHLREKIEIDPAEPRYLKV
                     VWGLGYKIEKGGA"
BASE COUNT          882 a         1367 c         1184 g          830 t
ORIGIN      
        1 cgaccgtggc aaacggacaa cccctacact tgcagcctga gaagtgtttt ccccgacgca
       61 catgccgtcg gggaaaacac ttcggaacct gattcgcacc tgcggacgtg aactctgcaa
      121 ggctttacaa tctgaaccgc ccggccttag ccgggcggct gtgctctctc ctcctcatca
      181 gatatgcagg gccgtccgca ccaggaagat gatgaacagg tgggccgggt agaaggcgta
      241 gaagaaccac ttaggaatct tgggctccac gtgggtgggc aggaagatca gcggcgcgga
      301 caggagggca aagatctcaa agccgatggc gtgtcccccc accaccaggg ccatcgggtc
      361 ctccagggac ccgccccaga gagccgggag gtagctcagc acatagagca gcgcccccag
      421 ccagggcttg ttccggcaca ggtagaagat cagggtcagg atgacgcccg tgttggcgta
      481 gtccgcgttc agaacgttga tggccagcac cccgaggagg aaccaccacc acttccgctc
      541 cttcacgccg tagatggcca gcaggctgaa aaacagagtg acaaagatgt tccagctggt
      601 gagctcttcc cagaagttgt ggggatggaa ggccaggacg aagaagggct gggagaccac
      661 tgcaaagagc gccagccgcc ccagataccg cctgatgtcc cgtgtgtaca tcaggccaac
      721 tgtcaggcag tagcagaaaa tcgggaaggc cagccgtcct acccaccgga attccgggtg
      781 ctgggggaaa aagacggtcc ccacgtggtc caccaccata gtcaggatgg cgaacagctt
      841 caaaaagttc gtgtccaggt tggtcctaag ctttggcgtc tgtctctgtg tctctgcgtc
      901 catgttcgat actcctccat ttgtgttctt caccgcaccg gccccggggg ctcccgcccc
      961 cgggcggcgg gcgaaaaaca aaaacgccgt gcagggcaag cctacacagc gcgagaagca
     1021 cgggcttctc catgcaacac ggacgcagga gtacaacagc aaaaccgacc tttacaagaa
     1081 agaccggaat gtcgtataat atccatgcgg tgcggccgga tggccgttgt gtaggtggtg
     1141 tcagcacctg cgcaggtttg cgggcgttgg cgcgcccgca aactgccgca tttttgtttt
     1201 tcgtgttgat tatagcagga aaccagttcc aatgcaagag gcaggcacac acgccctttt
     1261 tcgcgcagag gacagaaaga gaggagaggc ggatgcctct cctctctctc cgctcagccc
     1321 gactggggca tggtcacggt ggccttgaac aggtccccgt ccaccgccag ctcgaaggtc
     1381 ccgccctgga gctccgtcag gctgcgggcg atggacagcc ccagcccgga gccctcggtg
     1441 gtccgggact cgtccccccg gacgaagcgc tccatcaacc gctccggcgg cacgttcagc
     1501 gggtcccggg agatgttctt gatggacagg gtcacctgcc ccttcccccg gaccagctcc
     1561 agatagatgc gggtcccctc catggcatat ttggcacagt tgcccagcag gttgtcaatg
     1621 acccgccaca gatggcgccc gtccgcatac acataggtct ccccctccgg cagactgggg
     1681 accactgtga gcttccgggc ctccagcttc tcctcgtact cccccagagc ctggtccacc
     1741 agctggcaca ttccgatctt ctcccggttc accgtcagcg cgcctgtgga ggccttggac
     1801 gcctccacca gatcctctgt gagctttttc agccgctggg acttccgatc cagcacctcg
     1861 atgtattccc gggcttttgg gttttccacc tcctccattt tcaggaggtt gacataattt
     1921 ataatagagg tgagaggagt cttcagatcg tgggagacgt tggtgatgag ctccgccttg
     1981 aatcgctcac tcttcatctt ctcctccacc gcaccgttga gcccgtcagc aatgttgttg
     2041 agcgcctcgg cgtggagcct gagatcgtgg ggcatgcggg tagtgtcgat ctggtggttc
     2101 aggttgccgc ccgcgatggc ctcgctcccc ttccgcaggc gctggaagca gacactccag
     2161 tggcacagcc acagcagcat ccccagattg atcagaaaca gagggatggg attgtcccac
     2221 cagatgcggc gggcaaaaaa gcatagcacc agataggcca aaaaccccgc cacgctcctc
     2281 catatcatgg ggaaggagcg gatggcatcc agcagaccgc tccatatcca gctgcatgcc
     2341 cggcaaacca gggtggtgcg ggccaggacc cccgccttgc agcgcaccac cagggtgcgc
     2401 aggaccagca cggccagcgc cgtcatagcc gttacgcaca gagagaccac ggtggcgctc
     2461 acctggttct catagctctc ctgagacgca tacccccagc cgtacaggaa ctccatcagc
     2521 gggaaaatca gaatgaaaaa caggaccccc gccccgatca gatacaggtc aaaatagatt
     2581 ttttcctgcc aagtgaggac gatcccctcc actccctcct tatggccgga ggaccacatg
     2641 agaaaggcaa agcaggcaag ggagagcacc aggcacacac aggtggcagc gatcctcagg
     2701 gggatctctt tccggccccc tgtgtcgcgg gcatggagct cagaaaattc atcctctgcc
     2761 ccggacagct cctgggggac gccacactcg atgatataat aggcatctcc gctctcctcc
     2821 gacccctccg tcaccagcgc agggtccccc gcatcggggc tgctgttcca gcgctgggtc
     2881 aggtgctccc ctggacggaa cacagcggag tggatctcgg gcaccagctg ctccagggac
     2941 tcccccggcg ccagattggt ggctaggacc tttgtcccgt cctgactgag cacccggtag
     3001 cggaaagagg tctgatcctg cgccattgct gctccgatcc gctccgcatc tgtctcatac
     3061 cgcacctggt ccagataggc cagtccagga tcctccagcg cggctctggc cgcgaaccac
     3121 tcggccagct ggtcctcccg ttcccggatc agctctttga actcccaggt atcctgccag
     3181 ctgtctgtca gcacatagtc gatcccccgc agcacagcca tcccctgcca gcacaggctg
     3241 aagacggaca ccagaatcag cagtgaggcg atcaccttgg ccggcacgct ctttcggaca
     3301 tcgctcactc acgctccccc tttctcgatc ttatagccca gcccccacac tactttcaga
     3361 tagcggggct cggcggggtc gatctcgatc ttctcccgca gatgccggat gtggaccgcc
     3421 accgtgttct ctgagccgta ggcggggtcg ttccatacct gttcatagat ctgggcagag
     3481 gaaaagacct gtcccgggtg ggagaggagc agcttcaaaa tgttatactc aatgggcgtc
     3541 agggacacag gcgcaccgtc caccatcacc tgcttggacc cgtcgtccat agagatgggg
     3601 cccacggtga gcataccggg cgccttctcc gctcccccca gtgccgtata gcggcgcagc
     3661 tggctcttca cccgggcgat cacctccagg ggattgaagg gctttgtaat gtagtcgtcc
     3721 gccccgatgt tcagtcccag gatcttgtct gtatcctcgc tcttggcggt aagcaggata
     3781 atgggcacat tgctcccctc ccgcagcttg gccgtggccc ggatgccatc cagctcaggc
     3841 atcatcacgt ccatcaggat cagatggatc tcgttttcct ccgccgcctg gatagcctcc
     3901 cggccgttat atgccgcgat ggtgcggtac ccctcgctgg tcaggtagat atccagtgcg
     3961 gagacgatgt cccggtcatc gtcacaaatc agtatcgtgt acatactctc tcccccagaa
     4021 gcttcatttc cggccggctc agcaggtccg gcccgactgt actataagga tagcatagac
     4081 agtttaagtt gcaagaccga aaatcttaag aactgataaa attgtggggc catccctgtg
     4141 ctttcttccg ctcctccgcc ggtaaggtcc ccttccaatg cgggcttcct aaccggtttt
     4201 ttaaatttat cctatttcct ccaaaaacac accaacaaac ctcataagaa aagggctttt
     4261 ttc
//
LOCUS       DBMDSJ010000100         4227 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5550_length_4227_cov_1.987397, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000100 DBMDSJ010000000
VERSION     DBMDSJ010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5550_length_4227_cov_1.987397"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="ACZPAQ_03565"
     CDS             <1..387
                     /locus_tag="ACZPAQ_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR0903092.1"
                     /translation="RGEERTMIFYEAPHKLVATLRDLADTFGREREISLCRELTKLHE
                     EIRRTTLGEAADWYGEETPRGEFVLVVRGAQPEEEGECTLEDGLTLAERLREEEGLSL
                     RDAVRQAAKELGLSRNELYRLAVEQE"
     gene            complement(434..1315)
                     /locus_tag="ACZPAQ_03570"
     CDS             complement(434..1315)
                     /locus_tag="ACZPAQ_03570"
                     /EC_number="3.1.30.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIR0903093.1"
                     /translation="MKIRTLHLIPLLLLLCAALSGCGQLLQAAPSSFSLEDVPPFSGE
                     PYVVLQDNQPGFSQEDFTTDSFETYSSLDLLGRCGTAYANIGLDLMPTEERDSIGQVK
                     PSGWQTVKYDIVDGKYLYNRCHLIGYQLSGENANEQNLITGTRYLNVEGMLPFENMVA
                     DYVQETGNHVLYRVTPIFQGTELVARGVQMEAESVEDQGEGICFNVYVYNDQPGITID
                     YATGASSLEGDALPEDSTQKEPEDTLYILNTSSGKFHLPFCSGAASMKEENRQAFSGS
                     REELIAQGYSPCGQCKP"
     gene            complement(1312..1734)
                     /locus_tag="ACZPAQ_03575"
     CDS             complement(1312..1734)
                     /locus_tag="ACZPAQ_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903094.1"
                     /translation="MILISCIDDRGGLLFHGRRLSRDRVLCQDVLRTCAGSPLWMAPG
                     SRSLFSSLRDGLSSSILTSEDFLAQAAPGEFCFLEDRPIRPFLDRVESMVLYHWNRRY
                     PADLFLDLDPAALGWSLLGREEFPGSSHKKITKEVYRP"
     gene            1945..2607
                     /locus_tag="ACZPAQ_03580"
     CDS             1945..2607
                     /locus_tag="ACZPAQ_03580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR0903095.1"
                     /translation="MELETKELLDLSHTLAGEYLAGFQYPWQALDGIKELILTLGAAL
                     PPEEYDQPAPQVWVHKTAVVFPTAYLGAPCIIGPETEVRHCAFIRGSALVGAHCVVGN
                     SVELKNVILFDNVQTPHYNYVGDSILGYKSHMGAGSITSNVKSDKTLVTVKNGAERIE
                     TGRKKFGAILGDHVEVGCNSVLNPGTVVGRGSSIYPVSCVRGAIPAGSIVKTGGVVVQ
                     RS"
     gene            complement(2811..3062)
                     /locus_tag="ACZPAQ_03585"
     CDS             complement(2811..3062)
                     /locus_tag="ACZPAQ_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR0903096.1"
                     /translation="MKSTGIVRKVDELGRIVLPIELRRTLDIAEKDSLEIYMDGPSII
                     LKKYQPACIFCDDARDVIVFRGKNICPKCIKALQECGQF"
     gene            3336..3914
                     /locus_tag="ACZPAQ_03590"
     CDS             3336..3914
                     /locus_tag="ACZPAQ_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR0903097.1"
                     /translation="MTMTILWTGMVVLSILCGLATGQSAQVAAAAVEGTQAAVELCLS
                     IAGMLCLWTGVMEVMRRSGLAEKLSRLLRPMLRLLFPQVARDRETMDSIAANVSANLL
                     GLGNAATPLGLEAARRMSRCSPGVASDALCMLVVCNTASIQLIPTTVATVRAGAGSAA
                     PFDILPAVWLASGLSVGVGVLACKICARIWRD"
     gene            3916..>4227
                     /locus_tag="ACZPAQ_03595"
     CDS             3916..>4227
                     /locus_tag="ACZPAQ_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIR0903098.1"
                     /translation="MLDLMMTLVVPLTLGLVAVYAACRRVDVYDALIQGAGEGLGTLA
                     RIIPSLVGLMTAVYMLRASGALDLAAQALAPLLDRVGVPAELLPLMLVRPISGSAALG
                     VG"
BASE COUNT          820 a         1134 c         1468 g          805 t
ORIGIN      
        1 cggggggagg agcggaccat gatcttctac gaggcccccc acaagctggt tgccaccctc
       61 cgggacttgg cggacacttt tggccgggag cgggagatat ctctgtgccg ggagctgacc
      121 aagctccatg aggagatccg gcgcaccact ctgggggagg cggcggactg gtatggggag
      181 gagacgccac ggggagagtt cgtcctggta gttcggggtg cccagccgga ggaggagggg
      241 gaatgcactc tggaggacgg cctgacattg gcggagcggc tgcgggagga ggaagggctc
      301 tccctgcggg acgcggtgcg ccaggcggcc aaggagctgg ggctctcacg gaatgagctc
      361 taccgcctgg cggtggagca ggaataaaaa cggcccctgc ccaggaaagg gcaggggccg
      421 tctgctgtgg gggtcatggt ttgcactggc cgcaggggga atatccctgg gcgatcagct
      481 cctcacggct gccggaaaag gcctggcggt tttcctcttt catggaggcg gcgccggagc
      541 agaaggggag atgaaatttt ccagagctgg tgttcaggat gtacagggtg tcctccggct
      601 ccttctgggt gctgtcctct ggaagagcat ccccctccag ggagctggca ccggtagcgt
      661 agtcgatggt gatgccgggc tggtcattat agacatagac gttgaagcag atgccctcgc
      721 cctggtcctc caccgactcg gcctccatct gcacgccccg ggccaccagc tcagtgccct
      781 gaaagatggg agtgacccgg tagaggacgt ggttgccggt ctcctggacg taatccgcca
      841 ccatgttttc aaaggggagc atcccctcca cgttgagata gcgggtgccg gtgatgaggt
      901 tctgctcatt ggcattctca ccgctgagct gatagccgat gagatggcag cggttgtaca
      961 ggtacttgcc atccacgatg tcatatttca ccgtctgcca gccgctgggc ttgacttggc
     1021 cgatgctgtc ccgttcctcc gtgggcatca ggtccagacc gatgttggca taggcggtgc
     1081 cgcaccggcc aagcagatcc aggctgctgt aagtctcgaa agagtcggtg gtgaagtcct
     1141 cctgggaaaa gccgggctgg ttgtcctgga gcaccacata gggctcgccg gagaagggcg
     1201 ggacgtcctc cagagagaag gaggagggcg cggcctgaag gagctggccg cagccggaca
     1261 gcgccgcgca gagcagcagg aggaggggga tgagatggag cgtacgtatt ttcatgggcg
     1321 gtagacttcc tttgtgattt ttttatggga ggagccggga aattcctccc ggcccaacag
     1381 ggaccacccc agggcggccg ggtccaggtc cagaaacagg tctgcggggt agcggcggtt
     1441 ccagtgatag aggaccatgg actccacccg gtccaggaag ggccggatgg gccggtcctc
     1501 cagaaagcag aactcccccg gggcggcctg ggcgagaaag tcctccgagg tcaggatgga
     1561 gctggagagg ccgtcccgca gggaggagaa gagggagcgg gagccggggg ccatccacag
     1621 gggggagccg gcgcacgtcc ggagcacgtc ttggcagagc acccggtccc ggctgagccg
     1681 gcggccatgg aacagcaggc cgccccggtc atcgatgcag gagatcagga tcatggtcca
     1741 ccctctttct tcggaaacag tacatttgtt ctggtgtgct tgttgattgt agcagagatg
     1801 gggccaaaat tccagaggaa aagaaaaaat ttttctccgt tccgagaaaa ggcggccctc
     1861 cctaggacct tgtatccggg gcgtccgatg tggtatgatg gagacgtccg gtacatgatc
     1921 aggactggaa aggaatgttt ggatatggaa ctggaaacaa aggaactttt ggacctctcc
     1981 cacaccctgg cgggggagta tctggcggga tttcaatacc cctggcaggc cctggacggg
     2041 atcaaggagc tgatcctgac cctgggggct gcgctgccgc cggaggaata tgaccagccc
     2101 gccccccagg tctgggtgca taaaacggcg gtggtgttcc ccacagccta tctgggggcg
     2161 ccctgcatca ttgggcctga gactgaggtg cgccactgtg cgtttatccg gggctcggcc
     2221 ctggtggggg cccactgcgt ggtgggcaat tccgtggagc tgaaaaacgt gatcctgttc
     2281 gacaacgtgc agacccccca ctacaactat gtgggagact ccatcctggg ctataagagc
     2341 catatgggtg ccggctccat cacctccaac gtgaagtcag acaagacgct ggtgacagtg
     2401 aagaacggag cagagcggat cgaaacgggc aggaagaagt tcggcgccat cctgggcgac
     2461 catgtggagg tgggctgcaa ctccgtgctg aatccgggga ccgtggtggg gcgggggagc
     2521 agcatctacc cggtctcctg cgtccggggt gccatcccgg cgggcagcat cgtcaagacc
     2581 gggggcgtgg tggtccaaag gagctgagtg ccgcggggga tgcggaacaa gacagagggc
     2641 gcggcgggat ctgccgcgcc ctcaagctgt ttgtgggagc cctctctccc acggaccggc
     2701 ggaggcggag accaaaagaa aaacgcccca aatcctggct ggatccagag cgtttaaaaa
     2761 accgcataaa atttgtctgc tgtttctatc tgggctgctg ggggatcaaa tcagaactgt
     2821 ccgcactcct ggagtgcctt gatgcacttg gggcagatat tcttgccgcg gaaaacgatg
     2881 acatcccggg cgtcatcaca gaaaatgcag gcgggctgat attttttcag aatgatggac
     2941 ggtccatcca tatagatctc cagggagtct ttctccgcga tatccaatgt acggcgcagt
     3001 tcgatgggaa ggacgatacg gcccagctca tcgaccttcc ggacaatgcc ggtagatttc
     3061 aatgtgatca ttcctttctt ccttcttcat gtcccagtcg tcaaaacgat cgatgcggtt
     3121 tcgcagtcat aatgtacgat tttggaaact aaacattatt atactacaaa gtgcgactgt
     3181 gtcaaccata atttgtgaag attctgcaaa aagtgttcgg aaagagcaaa aaatgatggg
     3241 aaaacaggga aatcagaaaa atgaacacca cggcagagaa tggttatctt ttccagacat
     3301 gtcccgtata tatgatttga aggggggaca ggggtatgac gatgacgatc ctctggacgg
     3361 ggatggtggt gctctccatc ctgtgcggac tggctacggg gcagtcggcc caggtggccg
     3421 cggcggcggt ggaggggacc caggcggcgg tggagctgtg cctgtccatt gcggggatgc
     3481 tgtgcctgtg gaccggggtg atggaggtga tgcggcggtc cgggctggca gaaaagctgt
     3541 cccggctgct tcgaccgatg ctccggctgc tctttcccca ggtagcccgg gaccgggaga
     3601 ccatggacag catcgcggcc aatgtgtcgg ccaatttgct ggggctgggc aacgctgcca
     3661 cccctctggg gctggaggcc gcccggcgga tgagccgctg cagccccggg gtggcttccg
     3721 atgcgctgtg catgctggtg gtgtgcaata cggcctccat ccaactgatc cccaccacgg
     3781 tggccacggt gcgggctggg gcagggagcg ccgctccctt tgacatcctg cctgccgttt
     3841 ggctggcctc cgggctgtcg gtaggggtag gagtgctggc ctgcaagata tgcgcgcgga
     3901 tatggaggga ttgacatgct ggacctgatg atgaccctgg tggtgccgct gacgctgggg
     3961 ctggtggcgg tctacgccgc ctgccgccgg gtggacgttt acgatgccct gatccaggga
     4021 gcaggggagg gactgggcac cttggcccgt atcatcccca gcctggtggg gctgatgacg
     4081 gcggtgtata tgctgcgggc gtcgggggcg ctggatctgg ccgctcaggc cctggctccc
     4141 ctgctggacc gggtaggggt gccggcagag ctgctccctc tgatgctggt gcgccccatc
     4201 agcgggagcg ccgccctggg ggtgggg
//
LOCUS       DBMDSJ010000101         4212 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5562_length_4212_cov_2.159329, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000101 DBMDSJ010000000
VERSION     DBMDSJ010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5562_length_4212_cov_2.159329"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(182..406)
                     /locus_tag="ACZPAQ_03600"
     CDS             complement(182..406)
                     /locus_tag="ACZPAQ_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903099.1"
                     /translation="MKRSHFGVMLFLTGSLGYLIFSLYLSQNQYTYNGMTGTLAALLG
                     NELLIPYLVFCAMGVIGFVICLYEAFIRKP"
     gene            complement(414..2222)
                     /gene="lepA"
                     /locus_tag="ACZPAQ_03605"
     CDS             complement(414..2222)
                     /gene="lepA"
                     /locus_tag="ACZPAQ_03605"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR0903100.1"
                     /translation="MTDRSLIRNFSIIAHIDHGKSTLSDRLIELCDAVPQREMESQLL
                     DNMDLERERGITIKARAVRLDYKAEDGRTYALNLIDTPGHVDFNYEVSRSLAACEGAV
                     LVVDSTQGVEAQTLANTYLALEHDLEILPVFNKIDLPAADPQKAKHEVEDIIGLPALD
                     APEISAKQGINIPAVLEDIVQNVPAPAGDPDAPLKALIFDSQYDPYLGVIVYFRVMEG
                     TLKKGMGVKMMASGASYQVLDCGYLRPLGMDSCQQLTAGEVGWFTASIKNVKDTQVGD
                     TITGADCPSAAPLPGYRPAQPMVFCGIYTEDGSKYPDLRDALDKLQLNDASLSFEPES
                     SIALGFGFRCGFLGMLHMEIIQERLEREFDLDLVTTLPSVIYEVTKTDGTVVRVDNPH
                     NYPDPGAILEAREPYAKVSIISPPDYVGNIMPMCQDRRGVFKDMQYLDTNLVELHYSM
                     PIGEIIYDFFDALKARTKGYASLDYELEGYEPSELVKVDLLLNGDQVDALSFIAHRDK
                     AYARARRICEKLKENIPRQLFEVPVQAAIGGKIIARETVKAMRKDVLAKCYGGDITRK
                     KKLLEKQKEGKKKMRTLGTVQLPTEAFMAVLKLDEE"
     gene            complement(2318..>4212)
                     /locus_tag="ACZPAQ_03610"
     CDS             complement(2318..>4212)
                     /locus_tag="ACZPAQ_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR0903101.1"
                     /translation="RVTYQVALNTDAMEKNRNDILLALIRIARDAGVEWEDTLPITAQ
                     PPFRYTTDTPFQYPTTDEEGSPTTSLTRLGRLAVKMKWIDDPTADGAQGAPLPTAEEL
                     LEKMRESFQLELEGADMRAVAGVLYELYYRSMVNSWPPYVFAEGVDIDFISKVKEQGL
                     SGVEIEAATVRTYNTEYAAHLLGRVGAIENWDAYKDLDLDGDGTPDYEMDDTVGKEGA
                     ELAFESYLRGTAGVREVERNTSGKVVSEKWTTAPQPGDNVVLTIDIDLQKQVEDILSQ
                     AIPQLASEDTEGAACVVMDVNRAEVLASASYPSYHLATYSADLAENSADPLKPFLNRA
                     FQGVYAPGSTFKMVTAVAGLESGIIEPDTEIMDTGVYTYYQDDGPQCWYWRQYRRKHG
                     LVNVSEALEVSCNVFFFDVGRRVGIQGLQEFAAKFGLGEPTGIELYEETGVMAGPEYT
                     QSMGQTWYEGSTLSVAIGQESSQFTPLQLANYIATLVNGGTRYSAHLLKEVKSSDFSQ
                     VLYTYEPEVLDSIDIQPENLDAVKAGMLALTTGKGSLARYFQDLPVQVGAKTGSAQVN
                     AETESNAVFVCFAPYDDPQIAISLVVEKGGSGSTLASIAADILRYYFSAEESREETLT
                     ENTLIR"
BASE COUNT          772 a         1234 c         1357 g          849 t
ORIGIN      
        1 gctttcctta ctgtcgccat tataccactt cttcctctcc cgtccaatac ttcatttcaa
       61 tggtcttgat agaagttttt tatcctgaca ctggaacacc aggctggaga gaaaaaagca
      121 ccttcctccg gcgtatggga cagcgcggga gggaaggtgc ttttggcagg gagggatatc
      181 cttaaggttt acggataaag gcctcgtaca ggcagatgac aaatccaatg acccccatag
      241 cgcaaaaaac gaggtagggg atcagcagct cgttgccaag cagcgctgca agggtgcccg
      301 tcatgccgtt ataggtatat tgattttggg acagatacaa gctgaagata aggtatccga
      361 ggctgcctgt cagaaacagc ataacgccga aatgggatct tttcataggt gtctcactcc
      421 tcgtccagct tgaggactgc catgaaagct tcagtgggca gttggactgt gcccaaggta
      481 cgcattttct ttttgccttc cttttgcttc tccagcagct tcttcttgcg ggtgatgtcg
      541 ccgccgtagc acttggccag cacgtccttc cgcatggcct tgacggtctc ccgggcgatg
      601 atcttgccgc cgatggccgc ctggaccggg acctcaaaga gctggcgggg gatgttttcc
      661 ttcagcttct cgcagatgcg tcgggcccgg gcataggcct tgtccctgtg ggcgatgaag
      721 ctgagggcgt ccacctggtc tccattgagc agcaggtcca ccttgaccag ttcgctgggc
      781 tcatagccct ccagctcgta gtccagggag gcatagccct tggtgcgggc cttcagggcg
      841 tcaaagaagt cgtagatgat ctcgccgatg ggcatggagt agtgcagctc caccaggttg
      901 gtgtccagat actgcatgtc cttaaacacg ccccggcggt cctgacacat gggcatgatg
      961 ttgcccacat agtcgggggg agagatgatg gagaccttag catagggctc ccgggcctcc
     1021 aggatggcgc cgggatcggg gtagttgtgg gggttgtcca cccgcaccac ggtgccgtcc
     1081 gtcttggtga cctcgtagat cacggagggg agggtggtga ccaagtccag gtcgaattcc
     1141 cgctccagcc gctcctggat gatctccatg tggagcatgc ccaaaaagcc gcagcggaac
     1201 ccaaagccca gggcaatgga ggactcgggt tcgaaggaca gggaggcgtc gttgagctgg
     1261 agcttatcca aggcgtcccg cagatcagga tatttggagc cgtcctcggt gtagatgccg
     1321 cagaagacca tgggctgggc cggccgatag ccggggaggg gggctgcaga gggacagtcg
     1381 gcgccggtga tggtgtcacc cacctgagtg tcctttacgt tcttgataga ggcggtgaac
     1441 cagcccacct caccggcggt gagctgctgg caggagtcca tgcccagggg gcggagatac
     1501 ccgcagtcca gcacctgata gctggcaccg gaggccatca ttttgacccc catgcccttt
     1561 ttcagcgtgc cctccatcac ccggaaatag acgatgacgc ccagataggg gtcatactgg
     1621 ctgtcaaaga tcagggcttt cagaggggcg tccgggtcgc cggcgggggc ggggacgttc
     1681 tgcacgatgt cctccagcac agcggggatg ttgatcccct gcttggcgga gatctccggc
     1741 gcgtccaggg cgggcaggcc gatgatgtcc tccacctcgt gcttggcctt ctgggggtcg
     1801 gcggcgggga ggtcgatctt gttgaacacg ggcaggatct ccaggtcgtg ctccagggcc
     1861 agataggtgt tagccagggt ctgggcctcc acaccctggg tagagtccac caccagcacc
     1921 gcgccctcgc aggcggccag ggaccgggac acctcatagt tgaagtccac gtggcccggg
     1981 gtgtcgatca ggttcagggc ataggtcctg ccgtcctccg ccttgtagtc caggcggacg
     2041 gcccgggcct tgatggtgat gccccgctcc cgctccagat ccatgttgtc cagcagctgg
     2101 gactccatct cccgctgggg gacggcgtca cacagctcaa tgagccggtc agacagcgtg
     2161 gattttccgt ggtcgatgtg ggcgatgatg gaaaagttgc ggatcaaaga tcggtcagtc
     2221 ataaaaggaa acctccttgg gaagatccac ggccccgtgg aagcggggcg tgtgggaaga
     2281 agagggctct actccgatag gatcagcggc ccctatttta ccggataagg gtgttctccg
     2341 tcaaggtctc ttcccgggac tcctcggcgg agaagtagta gcgcaggatg tccgccgcga
     2401 tgctggccag agtggagccg ctgccgccct tctccaccac cagggagatg gcgatctgcg
     2461 gatcgtcata gggggcgaag cagacgaaaa cagcgttgga ttccgtctcg gcgttcacct
     2521 gggcggagcc ggtcttggcg cccacctgga caggaaggtc ctggaaatag cgggccagag
     2581 agcccttccc ggtggtcagg gccagcatcc ccgctttcac cgcatccaga ttctccggct
     2641 ggatgtcgat ggagtccagc acctccggtt cgtaggtgta aagcacctgg gagaagtcgc
     2701 tggatttgac ctccttgagg agatgggcag agtaccgggt gcccccgttg accagggtgg
     2761 cgatgtagtt ggccagctgg aggggggtga actggctgct ctcctggccg atggccaccg
     2821 acagggtgct gccctcgtac caggtctgcc ccatggactg agtgtactcc ggtccggcca
     2881 tgacgccggt ctcctcatac agctcgatgc cggtgggctc cccgaggccg aatttggcgg
     2941 caaactcctg gagcccctga atgcccaccc ggcggcccac atcgaagaag aatacgttgc
     3001 aggagacctc caacgcctcg gacacattca ccaggccgtg cttgcgccgg tactggcgcc
     3061 aataccagca ctgggggccg tcgtcctgat aatacgtgta gacgccagtg tccatgatct
     3121 cggtatccgg ctcaatgatc ccgctctcca gtccggcgac agcggtgacc atcttaaagg
     3181 tggagccggg ggcatagaca ccctggaaag cacggttcag gaagggcttg aggggatcag
     3241 cgctgttctc cgccaaatcg gcagagtagg tggccaggtg ataggagggg taggaggcgg
     3301 aggccagcac ctctgcccgg ttcacgtcca tcaccacgca ggctgcgccc tctgtgtcct
     3361 cactggccag ctggggaata gcctgggaga ggatgtcctc cacctgcttc tgaaggtcga
     3421 tgtcgatggt gagcaccacg ttgtcccccg gctggggggc ggtggtccac ttctcggaca
     3481 ccaccttgcc gctggtattc cgctccacct cccggacccc ggcggtgccc cgcaggtagg
     3541 actcgaaagc cagctcggcc ccctccttgc cgacagtgtc gtccatctca tagtccggcg
     3601 tgccgtcccc gtccagatcc aggtccttat atgcatccca gttctcaatg gcccccaccc
     3661 ggcccagcag atgggcagcg tattcggtgt tgtaggtgcg gacggtggcg gcctcgatct
     3721 ccacgccgga gaggccctgc tccttcacct tagagatgaa gtcgatgtct acgccctccg
     3781 caaagacata gggaggccag ctgttcacca tggagcggta gtacagctcg tacagtactc
     3841 cggccacggc gcgcatatcc gcgccctcca actccagctg aaagctctcc cgcatttttt
     3901 ccagcagttc ctcggcggtg ggaagggggg cgccctgggc cccgtcggcg gtggggtcat
     3961 cgatccactt catctttacc gccagccgcc ccaggcgggt gaggctggtg gtggggctgc
     4021 cctcttcatc ggtggtggga tactggaagg gggtgtccgt ggtgtagcgg aaggggggct
     4081 gggcggtaat ggggagggtg tcctcccact ccacgccggc atcccgggca atgcggatca
     4141 gggccaggag gatgtcgttg cggtttttct ccatggcgtc ggtgttcagg gcgacctgat
     4201 aggtgacccg gt
//
LOCUS       DBMDSJ010000102         4206 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5574_length_4206_cov_1.873569, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000102 DBMDSJ010000000
VERSION     DBMDSJ010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5574_length_4206_cov_1.873569"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="ACZPAQ_03615"
     CDS             complement(<1..198)
                     /locus_tag="ACZPAQ_03615"
                     /inference="COORDINATES: protein motif:HMM:NF038048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HIR0903102.1"
                     /translation="MKLANALSLRSELQTRIRQLESRLDNNALVQEGESPAEDPMELL
                     RELEEDYAQLEELIARINRTNS"
     gene            complement(220..684)
                     /locus_tag="ACZPAQ_03620"
     CDS             complement(220..684)
                     /locus_tag="ACZPAQ_03620"
                     /inference="COORDINATES: protein motif:HMM:NF021378.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR0903103.1"
                     /translation="MSRRRRGRITVQSQPTGATRAVRRMAGVVQVIFGLVFVVVAVTQ
                     IMPLSPLFSLPFLAMGALAVVMGVLSAVGKNGLPHRVGYDVETGIEEETIAGLMEDVD
                     KTDRPSAGTHDHIPSTALDAGERLKQLEDLKKAGLITQEEYQEKRREILSRL"
     gene            complement(723..>2247)
                     /locus_tag="ACZPAQ_03625"
     CDS             complement(723..>2247)
                     /locus_tag="ACZPAQ_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR0903104.1"
                     /translation="VEVNGKLQPRILSLRHILDEYIAYQEQVLIRRTRFDLKKAQERA
                     HLLEGLLIAEDNIDAVIKTIRESYDDARENLMARFGLDEVQAQAILDMQLKRLQGLEK
                     EKLEAEYAELEKRIAYYNQLLADESMLRGVLKDELSAIRDKYGDERFTEIQDVEDEID
                     IEDLIEEEQCVFTLTQAGYIKRTPVSEYAAQSKGGMGKKGITTREEDYVVDVFTASTH
                     DYILFFTDTGKVYRKKGYQIPESGKTAKGTNIINILQVESGERVQTMIHTRSIEDDGR
                     FLFMVTRSGTVKRLPANTLKNLRNNGIRALRMDEGDQLIAVRETDGQQRILIATHDGM
                     AVCFDENDVRPMGREAVGVRGIRLREGDYVVGAARAKADHEVLSITEKGYGKRTPVEE
                     YRVTNRGGIGIKNYMVTEKTGSVVGIKVVDGSEDLLLVTQSGILIRTPVDNIRTAGRA
                     TQGVIVMRFKEEGDQVISLALTDREETAGTQETEEGDPAGERAEEAPISEENQSEEE"
     assembly_gap    2248..2257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2258..3298)
                     /locus_tag="ACZPAQ_03630"
     CDS             complement(<2258..3298)
                     /locus_tag="ACZPAQ_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR0903105.1"
                     /translation="MSKKPQYDPEEIRFPDQKIEISPLVPEMESSYIEYAMSVIVGRA
                     LPDVRDGLKPVHRRILYAMYEDGLTADKPFKKSATCVGDVLGRYHPHGDQSVYDALVR
                     LAQDFSMRYMLVDGHGNFGSIDGDPPAAYRYTEARMSKLADEMLRDIEKDTVDWDPNF
                     DETRKEPKVLPSRFPNLLVNGSSGIAVGMATNIPPHNLREVINAVICVLDDPEAQLSD
                     LMEHIKGPDFPTRGIIMGRSGIRSAYATGRGRLMVRARHEFEEFGKDRVRIVITEIPY
                     QVNKRMLIKNMADQVEDKRLEGISDIRDESDRNGMRIVIELKRDANPQVVLNRLFAQT
                     QLQTTFAINMLAL"
     gene            complement(3398..>4206)
                     /locus_tag="ACZPAQ_03635"
     CDS             complement(3398..>4206)
                     /locus_tag="ACZPAQ_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR0903106.1"
                     /translation="AILDKALTANRAREAARKARESIRRKTALGGAAMPDKLRDCNEA
                     NPELTELYIVEGDSAGGSATQGRDSRFQAILPLWGKMLNVEKARADKVYGNDKLTPVI
                     TALGAGIGDEFDLNKLRYHKVIIMADADVDGAHIRTLLLTFFFRFMRPLIENGYVYSA
                     VPPLFKLTRGKQIRLAFSEEERDKISAELRGDNPNAKVDISRFKGLGEMNPHELWETT
                     MDPEKRTLRRIELDDAVRADEVFTVLMGEKVEPRKEFIERNAKYAVNLDY"
BASE COUNT          652 a         1339 c         1303 g          902 t
ORIGIN      
        1 gctgttggtc cggttgatcc gggcaatgag ctcctccagc tgggcatagt cctcctccag
       61 ctctctcagc agctccatgg ggtcctctgc gggagactcc ccctcctgga ccagggcgtt
      121 gttgtccagc cggctctcca gctgccggat gcgggtctgg agctccgagc gcagggacag
      181 cgcgttggcc agtttcatgg cattgtcctc ctttttccgt cacaggcggc tcaatatctc
      241 acgccgcttt tcctgatact cctcctgggt gatgagcccg gcctttttca gatcctccag
      301 ctgcttcagc cgctctcccg catccagggc ggtggaaggg atgtggtcgt gggtcccggc
      361 ggaggggcgg tccgttttgt ccacatcctc catcagaccg gcgatggtct cctcttcgat
      421 cccggtctcc acgtcatagc ccacccggtg gggcagtccg tttttcccaa cggcgctcag
      481 gacccccatc accacagcca gagcgcccat ggccaggaaa ggcagggaaa agaggggcga
      541 cagcggcatg atctgggtga ccgccactac cacaaagaca aggccaaaaa tgacctgtac
      601 cactcctgcc atgcggcgga ctgcccgggt ggccccggta ggctggctct ggacggtgat
      661 ccgtccccgt cttctccggc tcatgccctc tcctcctatc ctaattgtat tttatattct
      721 ggttattcct cttcggactg gttttcttcc gaaatggggg cctcctccgc cctctccccg
      781 gcggggtctc cctcctccgt ctcctgggtg ccggcggtct cctcccggtc ggtgagggca
      841 agggagatga cctggtcccc ctcctccttg aagcgcatga cgatgacgcc ctgggtagcc
      901 cggccggcgg tgcggatgtt gtccacaggg gtgcggatca ggatgccgct ctgggtgacc
      961 agcagcaggt cctcgctgcc gtccaccacc ttgatgccca ccacgctccc tgtcttctca
     1021 gtgaccatat agttcttgat gccgatgccg ccacggttgg tgacccggta ctcctccacg
     1081 ggggtgcgct tcccatagcc cttctcggtg atggacagca cctcatggtc cgccttggcc
     1141 cgggcggcgc ccaccacgta gtcgccctcc cgcaggcgga tgccccggac gcccacggcc
     1201 tcccggccca tgggccgcac gtcgttctcg tcaaagcaca ccgccatgcc gtcgtgggtg
     1261 gcgatgagga tgcgctgctg tccatcggtc tcccgcacgg cgatcagctg gtccccctca
     1321 tccatgcgca gggcgcggat tccgttgttc cgcaggtttt tcagggtgtt ggcgggcagg
     1381 cgcttcacgg tgccgcttcg ggtgaccatg aagaggaagc ggccgtcgtc ctcgatggac
     1441 cgggtgtgga tcatggtctg gacccgctct cccgactcca cctggaggat attgatgata
     1501 ttggtgccct tggccgtctt gccagattcc gggatctgat agcccttctt ccggtagacc
     1561 ttgcccgtgt cggtaaagaa gaggatatag tcgtgggtgg aggcggtgaa cacgtccacc
     1621 acgtagtcct cctcccgggt ggtaatcccc ttcttgccca tgcccccctt ggactgggcg
     1681 gcatactcac tgaccggggt gcgcttgatg tagcccgcct gggtcagggt gaagacgcac
     1741 tgctcctcct caatcagatc ctcgatgtcg atctcgtcct ccacatcctg gatctcggtg
     1801 aagcgctcgt ccccatactt gtcccggatg gcggagagct cgtccttcag cacgccccgg
     1861 agcatggact cgtcagccag aagctggtta tagtaggcga tgcgcttctc cagttcagcg
     1921 tactccgcct ccagcttctc tttctccaga ccctggagcc gcttgagctg catgtccagg
     1981 atggcctggg cctgcacctc gtccaagccg aagcgggcca tcaggttctc cctggcgtcg
     2041 tcgtagctct cccggatggt cttgatgacc gcgtcgatgt tgtcctccgc gatgagcagc
     2101 ccttccagca ggtgagcccg ctcctgggcc ttcttcaggt caaagcgggt gcggcggatg
     2161 aggacctgct cctgataggc gatgtactcg tccagaatgt ggcgcaggga gaggatgcgg
     2221 ggctgaagct tcccgttgac ctccaccnnn nnnnnnncag ggccagcatg ttgatggcaa
     2281 aggtggtctg gagctgggtc tgggcgaaca gccggttgag caccacctgg ggattggcgt
     2341 cccgtttgag ctcgatgacg atgcgcatac cgttgcggtc ggactcgtcc cggatgtcgg
     2401 agatgccctc caggcgcttg tcctccacct ggtcggccat gttcttgatg agcatccgct
     2461 tgttcacctg gtatgggatc tcagtgatga cgatgcgcac ccggtccttg ccgaactcct
     2521 cgaattcgtg gcgggcccgg accatcagcc ggccccggcc ggtggcgtag gcagaacgga
     2581 tgccgctgcg gcccatgatg atgccccggg tgggaaaatc ggggcccttg atgtgctcca
     2641 tcaggtcgga gagctgagcc tccggatcat ccagcacgca gatgacggca ttgatgacct
     2701 cccgcagatt gtggggcggg atgttggtgg ccatgcccac ggcgatgccg ctggagccgt
     2761 tgaccagcag gtttgggaac cgggagggaa gcaccttggg ctccttccgg gtctcgtcaa
     2821 agttggggtc ccaatccacg gtgtccttct caatgtcccg cagcatctcg tcggccagct
     2881 tggacatgcg ggcctcggtg taacggtagg ccgcgggggg atcgccgtcg atggaaccga
     2941 agttgccgtg gccgtccacc agcatgtagc gcatggagaa gtcctgggcc aggcggacca
     3001 aggcgtcgta gacgctctga tccccgtggg gatggtagcg tcccagcacg tcgcccacgc
     3061 aggtggccga cttcttgaag ggcttgtccg cggtgaggcc gtcctcatac atggcgtaga
     3121 ggatgcggcg gtggaccggc ttcagtccgt cccgcacgtc gggcagggcc cggcccacga
     3181 tgacggacat ggcatattca atataggaac tctccatctc gggcaccaga ggggatatct
     3241 cgatcttctg gtccgggaaa cggatctcct caggatcata ttggggtttt ttgctcatat
     3301 cttaactttc ctccaccttc atggattcaa cctgtacgat cagtgcggag ccgccgcggt
     3361 caaagtgtgg agacaggccg cccggcgtcc gctcttctca gtaatccagg tttactgcgt
     3421 acttggcgtt gcgctcgatg aactccttcc ggggctccac cttttcgccc atcaggacgg
     3481 tgaacacctc atctgcccgg acggcgtcgt ccagctcgat gcggcgcagg gtgcgcttct
     3541 ccgggtccat agtggtctcc cacagctcat gggggttcat ctcacccaga cccttgaagc
     3601 ggctgatatc caccttggcg ttgggattgt cccctctcag ctcggcggag atcttatccc
     3661 gctcttcttc actgaaggcc aggcggatct gcttgccccg ggtcagcttg aacagagggg
     3721 gcactgcgga gtagacatat ccattctcga tgagggggcg catgaaccgg aagaagaaag
     3781 tgagcagcag ggtgcggata tgagcaccgt ccacatccgc atcggccatg atgatgacct
     3841 tgtgatagcg cagcttattc aggtcgaact cgtctccgat gcccgccccc agggcggtga
     3901 tgacgggggt gagcttgtcg ttgccgtaca ccttgtccgc ccgggccttc tccacgttga
     3961 gcatcttgcc ccacaggggg aggatggcct ggaaccggga gtcccggccc tgggtggcgg
     4021 agcctcctgc ggagtcgccc tctacgatgt agagctcggt gagctcgggg ttggcctcgt
     4081 tgcagtcccg cagcttgtcc ggcatagcgg ccccgcccag agcggtcttg cggcggatgg
     4141 actcccgggc cttccgggcg gcctcccggg cccggttggc ggtgagggcc ttgtccaaga
     4201 tggccc
//
LOCUS       DBMDSJ010000103         4198 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5583_length_4198_cov_1.767879, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000103 DBMDSJ010000000
VERSION     DBMDSJ010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5583_length_4198_cov_1.767879"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="ACZPAQ_03640"
     CDS             complement(<1..1153)
                     /locus_tag="ACZPAQ_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR0903107.1"
                     /translation="MTFEELYRTMLKQAVEKGSVQADDLEASAGHLDCLLHPQDHPLV
                     WPLNPCTCPKEETHCAAACLFDAIVRRDDGTLAVDPDRCSGCGACIQACQSGNLTASR
                     DVLPALDAVKHAKGPVYALIAPAFLGQFSTVVTPGRLRSAFKLLGFTGMVEVALFADL
                     LTLKEALEFDRNIHTETDFQLTSCCCPMWIGMIRKLYHELMPHVPGAVSPMIAAGRTV
                     KKLHPDAVTIFVGPCIAKKAEAREADLAGAVDYVLTFQEMQDIFQAADIHPELLEESE
                     RDHSSRAGRIYARTGGVSEAVRTTVARLHPERPIQVRTRQADGVPACRAMIEDIRSGQ
                     HQANFFEGMGCTGGCVGGPKVLLDRETGRENVDRYGDEAEFSTPLDNPYV"
     gene            1429..3462
                     /locus_tag="ACZPAQ_03645"
     CDS             1429..3462
                     /locus_tag="ACZPAQ_03645"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR0903108.1"
                     /translation="MYRRETPWDIVKPLLFFLGSILWLEGIYRAFYVRPFFDVGLVYI
                     LLFSLPLAGMCALLTSLWGKKGNRKAGFAVLGVLTLWYMIQSVYHTIFATVLVTKSFN
                     MAGQALGSYWKETLLGIWEALPALLLLAVPLVVYFFQPRFALLNRPDGRVLLGVIGGV
                     IIVQAAAVLAIALPTGGIMTTRQIYRETFVPDLTVSHFGLATTLRLDIEQTLFGLEEP
                     SRPDPGEGSVSTLAPGNVSVQPEEPEEGKTPAASGPNVMDIDFDALIANETDSTLKDM
                     HEYFRDRTPTEKNQYTGMFQGKNLLLFTGEAFWIGAVNEQYTPTLYKLAHSGFVFENF
                     YNPLWYYSTVDGEYAATTGLIPTNQVNASQRYAGKNGVSMYFSMGNQLRALGYPTLAY
                     HNNTANYYDRNLSHPNLGYDFYACDTGLDVAMTWPQSDLEMMQLTLPQALAGEKPFHN
                     YYMTVSGHMNYSFAGNAMSAKHRDEVADLDMSEAARAYLACNMELDQALEYTIKTLEE
                     AGELENTVIVLSGDHYPYGLDGTGAIDELAGEGTEADPFEKYRSTLILWSGDMEEPVV
                     VDKPCYSVDIIPTLSNLFGLEYDSRLLAGRDILSDAPGLVIFNDYSYLTELGRYNADT
                     DTFTPNEGAQVPENYAQDTYWEARYIRSYSEKILFNDYYRKIGLEPPAGPEEN"
     gene            complement(3530..4066)
                     /gene="rbr"
                     /locus_tag="ACZPAQ_03650"
     CDS             complement(3530..4066)
                     /gene="rbr"
                     /locus_tag="ACZPAQ_03650"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR0903109.1"
                     /translation="MPELKGSKTEQNLWTAFAGESQARNKYTYFASKAKKDGFVQISK
                     IFEETAANEKEHAEIWFKLLGGIGSTAENLAAAAEGENYEWTDMYATMAKEAEEEGFD
                     HIAFLFSEVAKIEKEHEERYRKLLANVEGGLVFSRDGDMIWQCSNCGHIHVGKQAPEV
                     CPVCAHPRDYFQLKAENY"
BASE COUNT          812 a         1338 c         1207 g          841 t
ORIGIN      
        1 ggacataggg gttgtccagc ggagtgctga actccgcctc gtctccgtaa cggtctacat
       61 tctcccgccc ggtctcccgg tccagcagga ccttcggccc gcctacgcag ccgccggtac
      121 agcccatccc ttcaaagaaa ttcgcctggt gctggccgga gcggatgtcc tcgatcatcg
      181 ccctgcacgc cggcacgccg tctgcctgcc gggtccggac ctggataggc cgctccgggt
      241 gcagacgggc caccgtggtc cgaaccgcct cgctgactcc gccggtacgg gcgtagatcc
      301 gccccgctcg ggaggagtgg tcccgctcgc tctcctccag cagctccggg tggatatccg
      361 ccgcctggaa aatatcctgc atctcctgga aggtaagcac atagtccacc gcgccggcca
      421 ggtctgcctc ccgggcctcc gcctttttgg caatgcaggg ccccacaaag atggtgaccg
      481 catctgggtg cagctttttt accgtccggc cggcggcgat cataggggat accgccccgg
      541 gaacgtgtgg catcagctca tgatacagct ttcggatcat gccgatccac atggggcagc
      601 agcagcttgt gagctggaaa tccgtctctg tgtgaatgtt ccggtcaaac tccagcgcct
      661 ccttcaacgt cagcaggtcc gcgaagaggg ccacctccac catcccggtg aagcccagca
      721 gcttgaaagc cgagcgcagc cggcccggcg tcaccacagt ggagaattgg cccaggaagg
      781 caggggcgat cagggcgtag acgggccctt tggcatgctt gactgcgtcc agggcaggca
      841 gcacgtcccg gctggcagtc aggtttccag actggcaggc ctggatacag gcgccgcatc
      901 cggagcagcg gtccgggtcc acggcaagcg taccgtcgtc cctgcgcacg atggcgtcaa
      961 acaggcaggc ggcggcgcag tgtgtctcct ctttgggaca ggtgcagggg ttgagcggcc
     1021 agaccaaggg gtggtcctga gggtggagca ggcagtccag atgcccggca ctggcctcca
     1081 gatcatctgc ctggacggag cccttctcaa cagcctgctt cagcatagtg cggtaaagtt
     1141 cctcaaaggt catggtggtc ctccccttct gtcgatacag tggtcagtat gtccgttttg
     1201 ggaacaaaca aacccgtttc cctgttgttt ccctattata tcacagaggg actcggccgt
     1261 catccctatt ccccgggaaa cagggcagag aaaaagggag aaccctctgg acagaagtcc
     1321 cggcaggagg tatgataaag aggtaagcac tgcgggccgc ctggagtccg cgcacactga
     1381 gatcaagcat ctgccccggg tcgggcagag cagcgaggat ttgatatcat gtatcggaga
     1441 gaaactccct gggacatcgt aaaaccgctc ctgtttttcc tgggctctat cctctggctg
     1501 gaggggatct accgggcttt ctatgtccgg cccttttttg atgtggggct ggtctatatc
     1561 ctgcttttct ccctgccgct ggccgggatg tgtgcccttt tgacctctct ctggggcaag
     1621 aaggggaacc gcaaggccgg cttcgccgtg cttggcgtgc tgaccctgtg gtacatgatc
     1681 cagtcggtgt accacacgat ctttgccacc gtgctggtca ccaagtcctt caacatggca
     1741 ggtcaggccc tgggcagcta ctggaaggag accctgctgg gcatctggga ggccctgccc
     1801 gccctgctcc tgctggccgt ccctctggtg gtgtacttct tccagccacg gttcgccctc
     1861 ctgaaccgcc cggacggccg ggtgctgctg ggagtgatcg gcggcgtgat catcgtgcag
     1921 gccgcagcag tgctggcgat cgccctgccc accggcggga tcatgacgac ccgccagatc
     1981 tatcgagaga cctttgtccc agacctgacg gtctcccact tcggcctggc caccaccctg
     2041 cggctggata tcgagcagac cctctttggc ctggaggaac ccagccgccc cgacccgggt
     2101 gagggctcgg tctccacgct ggccccgggg aatgtctctg tccagccgga ggagccggag
     2161 gaagggaaga cccccgccgc ctccggtccc aatgtcatgg acatcgactt cgacgccctg
     2221 atcgccaacg agacggacag caccctgaag gacatgcacg agtatttccg ggaccggacg
     2281 cccacggaga aaaatcagta caccggcatg ttccagggca agaatctgct ccttttcacc
     2341 ggcgaggctt tctggatcgg cgcagtgaac gagcagtaca cccccacctt atacaagctg
     2401 gcccactccg gctttgtgtt tgagaacttc tacaaccccc tgtggtatta ctccacggtg
     2461 gatggggagt atgccgccac caccggcctg atccccacca accaggtcaa tgcctcccag
     2521 cggtatgcag gaaaaaacgg ggtctccatg tacttctcca tgggcaacca gctccgggcc
     2581 ctgggctatc ccaccctggc ctaccacaac aatacagcca actactatga ccgcaacctc
     2641 tcccacccca acctgggcta tgatttctac gcctgtgaca ccgggctgga tgtggccatg
     2701 acctggcccc agtccgacct ggagatgatg cagctgaccc tcccccaggc tctggccgga
     2761 gagaagccct tccacaacta ctatatgacg gtgagcggcc acatgaatta cagcttcgcg
     2821 ggcaacgcca tgtccgccaa gcaccgggat gaggtggcgg atctggacat gagcgaggcc
     2881 gcccgggcct atctggcctg caacatggag ttggaccagg ctctggagta caccatcaag
     2941 acactggagg aggccggcga gctggagaac accgtgatcg tcctctccgg ggaccactac
     3001 ccctatggcc tggacggcac cggcgccatc gacgagctgg cgggggaagg gacggaggcc
     3061 gatccctttg agaagtaccg ctccacgctg atcctctggt ccggtgacat ggaggagcct
     3121 gtggtagtgg acaagccctg ttacagcgtg gacatcatcc ccaccctgtc caatctgttc
     3181 ggcctggaat atgactcccg cctgctggcc ggccgggata tcctctccga cgcccctggg
     3241 ctagtcatct ttaacgatta cagctatctc accgaactgg ggcgttacaa tgcggacacg
     3301 gacaccttca ccccaaatga gggggcgcag gtgccggaaa actacgccca ggacacctac
     3361 tgggaggccc ggtatatccg cagctattcg gagaagatcc tattcaatga ctactaccgc
     3421 aaaatcgggc tggaaccccc ggccggtccg gaggagaact gaccagacgc agaaaggacg
     3481 cgcctcaatg cgaggcgcgt ccttttagag atatgtatgg atcagcagat caatagttct
     3541 ccgccttcag ctggaagtag tcccggggat gagcgcagac ggggcagacc tccggcgcct
     3601 gcttgcccac atggatatgg ccgcagttgg aacactgcca gatcatatcg ccgtctctgg
     3661 agaacaccag gcccccctcc acgttggcca gcagcttgcg gtaacgctcc tcatgctcct
     3721 tctcgatctt ggcaacttcg gagaacagga atgcgatgtg gtcaaagccc tcctcctcgg
     3781 cctccttggc catggtggcg tacatatcgg tccactcgta gttctcgccc tcagcagcgg
     3841 cggccaggtt ctccgcagtg gaaccgatcc cgcccagcag cttgaaccag atctcagcat
     3901 gctccttctc attggcggcg gtctcctcaa agatcttgga gatctggaca aaaccgtcct
     3961 tcttcgcttt ggaggcaaaa taggtgtact tgttgcgggc ctgggactcc ccagcaaagg
     4021 ctgtccacag gttctgttct gtcttgcttc ccttcagttc aggcatgatg atttcctcct
     4081 ttagtttctt aatgagaata attactgttt aaattatacc atatcctgcc cgcctcgtca
     4141 actcatttta cagatctttg gaaccatcct gtgtcttcga ccgggcccgc atctatta
//
LOCUS       DBMDSJ010000104         4171 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5620_length_4171_cov_1.820885, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000104 DBMDSJ010000000
VERSION     DBMDSJ010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_4171_cov_1.820885"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="ACZPAQ_03655"
     CDS             complement(<1..301)
                     /locus_tag="ACZPAQ_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR0903110.1"
                     /translation="MPIVSELILSLMLTASLSAGAPLSAPPGLAVSLPAITAQAQQLH
                     TPLMILHAGGVTPEGVTGSNSLEALEHSYENGFRLLELDFSWTCDGELVCVHDWNA"
     gene            537..974
                     /locus_tag="ACZPAQ_03660"
     CDS             537..974
                     /locus_tag="ACZPAQ_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR0903111.1"
                     /translation="MIPIPFTVLWLIALIVFAVGEAITVGLTSVWFAVGALGALIAAG
                     FGAALWLQVVVFLALSGVTLLLVRPLAKKFLTPRYQATNADRVIGATALVTEEIDNLK
                     GSGLVNISGQVWTARSAHDVVIQPGREVKVLRIEGVKVFVELL"
     gene            987..1964
                     /locus_tag="ACZPAQ_03665"
     CDS             987..1964
                     /locus_tag="ACZPAQ_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR0903112.1"
                     /translation="MDSILSFLVPIILVVLVLLVLAANVRVVPQSRAFVIERLGAFQG
                     VWGVGLHFKIPFIERVAKNVSLKEQVVDFPPQPVITKDNVTMQIDTVIYFQITDPKLY
                     TYGVENPMSAIENLTATTLRNIIGDLELDQSLTSRDHINGQMRAILDEATDNWGIKVN
                     RVELKNIMPPRDIQESMEKQMRAERERRESILQAEGQKQSQILVAEGEKQSAILRADA
                     AKQAAILQAEGAKQAKILEAEAQAQAIMKVQQATADAIKLINEANPGDGVLKIKALEA
                     FTAAANGKATKIIVPSEIQGLAGLAAAAKTVFDTEDPKPAAPKTTLPKQ"
     gene            complement(2087..4123)
                     /locus_tag="ACZPAQ_03670"
     CDS             complement(2087..4123)
                     /locus_tag="ACZPAQ_03670"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR0903113.1"
                     /translation="MTHLRSRWTALFLALVLSLSLALPASAAQEDRAQAAHEAAQYAM
                     QYGGAVSVQYALWENGEVTLQGHDGVYSKTENRALTDDDLYGIGSISKTYTAAAMMKL
                     VEQGKVDLDEPVTAYLPDFTMADPRYTQITVRMLLNHSSGLMGGTTNDAFLFGDQGND
                     DAPSRLLERLAGQTLQAEPGAYSVYSNDSFTLAQLVIERVSGMEYTAFLNQYLLEPLG
                     LENTLTPMEEFDTSRLVKTYLGTDTRALPAENLTIVGTGGIYSTASDLAAFGGALCGE
                     GLLSQASLDEMASDQYLLGMWPEDSDGDAVAYGLGWDSVHMFPFSQNGIQALVKGGDT
                     IVYHAGLVILPEYDMAAAVLTSGGISTYNQLAAARILLDALAEQGVKVEEEAALTPAQ
                     PAQMPAELTQLSGWYGTSTAAAQLQITDEGVLTLTGMEGTFTYREDGSFRDESDSVLL
                     KLVQEDNGETYLYQKSYASIPGLTTLCVASYAMERLPSYQPDEATQAAWEAREGKIYV
                     QTNERWSSAYYALSGVFAAVTLQGSPEGYLLTNQLTAPDTAAPVLQIPGTGSRDSAVI
                     RVLDVDGCETLELNGSLYQDASSVSPIFSGPESWCIIRPGQTARWYRTGEAAGSQMTV
                     EVPENGGFYVYDAALGLQASSWLYGDTTVVLPENGWIVFSGEEGAQFHIYLSQP"
BASE COUNT          797 a         1210 c         1397 g          767 t
ORIGIN      
        1 aagcgttcca gtcgtggacg cagaccagct ccccgtcgca ggtccaggag aagtccagct
       61 ccagcagccg gaagccgttt tcgtaggaat gttccagcgc ctccagactg ttgctgcccg
      121 tcactccctc cggggtgacg ccgccggcgt ggaggatcat caggggggtg tggagctgct
      181 gcgcctgagc ggtgatggca ggaagggaga cggccaggcc cggcggagca gagaggggag
      241 ctccggcgga cagggaggca gtgagcatga gggagaggat aagttctgaa acgatgggca
      301 tgggcgggat tctcctttaa gcgtcgagag agattggtaa aaaacagtat atcggatttt
      361 tcgccattag gcaaatactt tatagagcag aaaggcggcg ctcccccgat cctgaaatta
      421 tttacagaaa aaagagagaa aaccatagca attttttgta agatatgata tcctgtcatc
      481 caaggaaaga agttttcttt ccagcccccc gggatgggaa cagaaaggag tgtgctatga
      541 tacccatacc gtttactgtg ctctggctga tcgccctgat cgtgttcgcg gtgggggagg
      601 ctatcactgt gggcctgacc tccgtttggt tcgcggtggg tgctctgggt gcgctgattg
      661 cggcaggctt tggcgcggcg ctgtggctcc aggtggtggt ctttctggct ctgtccgggg
      721 tgaccctgct gctggtccgc cccttggcaa agaagttcct gacgccccgc taccaggcca
      781 ccaacgcgga ccgggtcatc ggcgccacag cactggtcac agaggagatc gacaacctga
      841 aggggagcgg cctggtgaat atctcgggac aggtctggac cgctcgctcc gcccatgacg
      901 ttgtcataca gcctgggcgg gaggtcaagg tgctccgcat cgagggagtc aaggtttttg
      961 tagagctgtt ataaggagga gagatcatgg atagtattct ttcgttttta gtgcccatca
     1021 tcctggtggt gctggtgctg ctggtgctgg ccgccaacgt ccgggtggtc ccgcagtccc
     1081 gcgcattcgt cattgaacgc ctgggcgcct ttcagggcgt gtggggcgtg ggcctgcact
     1141 tcaagatccc gttcattgag cgggttgcaa agaatgtttc cctgaaggag caggtggtgg
     1201 atttcccccc ccagcccgtc atcaccaagg acaatgttac catgcagatc gatacggtga
     1261 tctatttcca gatcaccgac cctaagctgt acacctatgg ggtggagaat cccatgtccg
     1321 ccattgagaa cctcactgcc accaccctgc gcaacatcat cggtgacttg gagctggacc
     1381 agtccctcac cagccgagac cacatcaacg gtcagatgcg ggccattctg gacgaggcca
     1441 ccgacaactg gggcatcaag gtcaaccggg tggagctgaa gaacatcatg ccgcccagag
     1501 acattcagga gtccatggag aagcagatgc gggccgagcg cgagcgccgt gagtccatcc
     1561 tccaggccga gggacagaag cagtcccaga tcctggtggc tgagggcgag aagcagtccg
     1621 ccatcctgcg ggctgacgcg gccaagcagg ccgccatcct tcaggctgag ggcgccaagc
     1681 aggctaagat cctggaagcc gaggcccagg cccaggccat catgaaggtg cagcaggcca
     1741 ccgctgacgc cattaagctc atcaacgagg ccaaccccgg cgacggcgtg ctgaagatta
     1801 aggccctgga ggcgttcacc gccgccgcca acggcaaggc caccaagatc atcgtaccca
     1861 gcgagatcca gggcctggcg ggcctggccg ccgcggcgaa aacggtgttc gacaccgagg
     1921 accctaagcc ggccgccccc aagaccaccc tgcccaagca gtaagacaca gacacgatcc
     1981 aggcgccgcc ccatggggcg gcgcctgttt ttatggccga cggtgcggtc cggctgacag
     2041 aaaaaaggaa cgctccggac gccggaaggg cggccggagc gtttgctcag ggttgtgaga
     2101 gatagatgtg gaactgggcc ccctcctcgc cggagaagac gatccagccg ttttccggaa
     2161 gaacaacggt ggtgtctcca tagagccagg agctggcctg gaggcccaga gcggcgtcat
     2221 acacatagaa accgccgttt tccgggacct ccaccgtcat ctgactgccg gcggcctcgc
     2281 ctgtccggta ccagcgggct gtctgccccg ggcggatgat gcaccaggac tccggcccgg
     2341 agaagatggg ggagacggag cttgcgtcct gatagaggga gccgttgagc tccagggtct
     2401 cgcagccgtc tacatccagc acacggatca cggcactgtc ccggcttccg gtgccgggga
     2461 tctgaaggac tggggcagcg gtgtcagggg cggtgagctg attggtgagc agatagccct
     2521 ccgggctgcc ctgaagggtg acggcggcaa agacgccgct taaggcgtag taggcggagg
     2581 accagcgctc gttggtttga acgtatatct tgccctctct agcctcccag gcggcctgag
     2641 tggcctcgtc cggctggtag ctgggcaggc gctccatggc gtaggaggcc acacagaggg
     2701 tggtcaggcc gggaatggag gcgtagctct tctgatagag gtaggtctcc ccgttgtcct
     2761 cctggaccag cttgagcagg acggaatcgc tctcgtcccg gaaactgccg tcctcccggt
     2821 aggtgaaggt cccctccatc ccggtaaggg tgagcacgcc ctcgtcggtg atctggagct
     2881 gggcggcggc ggtggaggtg ccgtaccagc cgctgagctg ggtcagctcg gcgggcatct
     2941 gggcgggctg ggcgggggtg agggcggcct cctcctccac cttgacgccc tgctcagcca
     3001 gggcatccag aaggatacgg gcggcggcca gctgattgta ggtgctgatc ccgccggaag
     3061 tgagcaccgc ggcggccatg tcatactcgg gcaggatgac cagtccggca tgatagacga
     3121 tggtgtcgcc ccccttgacc agggcctgga tgccgttctg actgaagggg aacatatgga
     3181 cgctgtccca gcccaggcca taggcgacgg cgtcgccgtc actgtcctcg ggccacatgc
     3241 ccagcagata ctggtcgctg gccatctcat ccaaagacgc ctggctcagc agaccctccc
     3301 cgcacagggc gccgccgaag gcggccagat cgctggcggt ggagtagatg cctcccgtgc
     3361 ccacgatggt caggttctcg gcgggcaggg cccgggtgtc agtccccaga taggtcttga
     3421 ccagccggct ggtatcgaac tcctccatag gggtcagggt gttctccagt cccagaggct
     3481 ccagcaggta ctggtttaga aaggcggtgt actccatgcc gctgacccgc tcgatgacca
     3541 gctgggccaa ggtgaagctg tcgttggagt agacgctgta agcgccgggc tcggcctgaa
     3601 gggtctgccc ggccagccgc tccagcaggc gggagggagc gtcatcgttc ccctggtccc
     3661 cgaacaggaa ggcgtcattg gtggtgccgc ccatcaggcc ggaggagtgg ttgaggagca
     3721 tgcgcaccgt gatctgggtg tagcgggggt ctgccatggt gaaatcaggg agataggccg
     3781 tgaccggctc atccaggtct actttcccct gctccaccag cttcatcatg gcggcggcgg
     3841 tataggtctt gctgatggag ccgatgccgt acaggtcatc atcggtgagc gcgcggttct
     3901 ccgtcttgga gtagacgccg tcgtgcccct ggagggtgac ctctccgttc tcccaaaggg
     3961 cgtactggac actgacggcg ccgccatact gcatggcgta ctgggcggcc tcatgggcgg
     4021 cctgggcccg gtcctcctgt gcggcggagg cgggcagggc cagagagagg gaaaggacca
     4081 gcgccaggaa cagggcggtc catctgctgc gaaggtgtgt cataggatct tcctttctga
     4141 tatggaggtt ggtttcatta tagcggaagc g
//
LOCUS       DBMDSJ010000105         4166 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5630_length_4166_cov_1.748339, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000105 DBMDSJ010000000
VERSION     DBMDSJ010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5630_length_4166_cov_1.748339"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="ACZPAQ_03675"
     CDS             <1..641
                     /locus_tag="ACZPAQ_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903114.1"
                     /translation="TLFTADGAFLDQLRTNGRRKNCRVELAQPDSGLPDFDFPENVAL
                     ALAVCREIGVDRDRALEGILRYQPDPYALSLFRLPSGAAFVNAMSVNDPQSTQLDYHR
                     VAGRPGMVGRRLVLLINNRPDRGYRTEHMMMVARGLEPEEIWLIGASQRAVRRTLRHI
                     LPDTPVRLFPGAEALPLDSRGADTMIFAAGNLAGPGKALMERVRKEGEQSVL"
     gene            631..1065
                     /gene="pgsC"
                     /locus_tag="ACZPAQ_03680"
     CDS             631..1065
                     /gene="pgsC"
                     /locus_tag="ACZPAQ_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HIR0903115.1"
                     /translation="MYYEIVLAGVAISLLFVELTGLSPAGLIVPGYLALCLQTPWRIV
                     YTLAVAVAAWGIAKLLGNVMILYGRRRFAVMVLLSFAIDLAVTSLGLLAYDPGMIGVL
                     VPGIMAQELERQGFLKSLVPLAAVVGILALIMMWQGMQVFPI"
     gene            1062..2204
                     /gene="pgsW"
                     /locus_tag="ACZPAQ_03685"
     CDS             1062..2204
                     /gene="pgsW"
                     /locus_tag="ACZPAQ_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HIR0903116.1"
                     /translation="MSRAKRERKLLALLLACALLAWGTAALAAGQGRRTETGYAAVQL
                     AAADLLEDCFRQVRTYKEELGIPLSELDYHQTGMIGESYTGITTTLGAIEAKRTTAWP
                     EMGALCVRLLHEAGVRSGDTVGAGFSGSFPAMDLAVIAACQVMGVELIYISSVGASTY
                     GANHPALTFPEMAHRLVEDGLLETDSAAVTLGGGGDVGGGMDPACAEEIRGRLAEAGL
                     LLLEEPDYRRNLELRQQLYQQEGPIDCFVAVGGNVTSMGRGESGISLGQGLISPEKIV
                     RLTEDSGLVQRYLSQGLPVINLLNIKQLAADYGLPYDPAQWPERGTSAVYFETDYPGA
                     VLLLGLAGAAVLLALCRWVRRSAGTARKEERGYHGQTESSADHPDH"
     gene            2164..2835
                     /locus_tag="ACZPAQ_03690"
     CDS             2164..2835
                     /locus_tag="ACZPAQ_03690"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0903117.1"
                     /translation="MDRQNRPLTILTIDDEEQIQYALRALFNSQGWEAISARDVEEGV
                     GKFRTCRPDLVLMDYHLPKINGVKGVEMLRREDPEVPLIVFTIEDNQRVADQFLDAGA
                     SDFVLKPVKALDIIARIKLHVRLVESRRENRQSTEEPVKGIGQGTLSLIEGYLRSVSE
                     YQPVEAIAEGTGLAYQTTYRYLQYLVSVNRVEVSQHYGKVGRPKQRYRMIREAGDGEK
                     PETGL"
     gene            3238..>4166
                     /locus_tag="ACZPAQ_03695"
     CDS             3238..>4166
                     /locus_tag="ACZPAQ_03695"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR0903118.1"
                     /translation="MGNRRRHIGSSLAALTLTLSLLTGLLTGCGPGGSQSSSPEAGST
                     PPAASQSTQTPEQPALPELTTDTYGNAAVGYQAAVVSANEYTSRIGMDILEAGGNAVD
                     AAVAMIFANSLTEPGATSLGGASFMTIYLKETGEYICIEAMETAPAAAGIDTLDEINE
                     KKGAMLVTVPGQVHGALTALEKYGTMTREEVLAPVIQLAEEGFDVHISFEERASASFD
                     KLVQNEEAAKIFTNEGLPYAVGDHFTNPDYADTLRKIAEGGIDEFYKGSIAQTIVDEV
                     QRLGGLLTMEDMASYTSVEREPISTTYHGYEIVT"
BASE COUNT          853 a         1167 c         1456 g          690 t
ORIGIN      
        1 ggaccctgtt cacggcagac ggagcgttcc tggaccagct tcggaccaac ggacgcagaa
       61 aaaactgccg ggtggagctg gcccagccgg acagcggcct gccggacttc gattttcctg
      121 aaaatgtagc tctggccctg gcggtctgcc gggagatcgg ggtggaccgg gatcgggcgc
      181 tggaagggat cctgaggtat caaccggacc cctatgccct gtccctattc cgcctgccct
      241 ctggggcggc ttttgtcaac gccatgtcgg tgaacgatcc acagtccacc cagctggact
      301 accaccgggt ggccggccgc ccaggaatgg tgggccggag gctggtgctg ctgatcaaca
      361 accggccgga ccggggatat cggacggagc acatgatgat ggtggcccgg gggctggagc
      421 cagaggagat ctggctgatc ggtgcctccc agcgggcggt gcgccgcacc ctgcggcaca
      481 tcctgccgga cacacctgtc cggctgtttc ctggagcgga ggcactgccc ttggacagcc
      541 ggggggcgga cactatgatc tttgccgctg gaaacttggc cggcccgggt aaggcactga
      601 tggagcgggt acggaaggag ggagagcaga gtgtattatg agatcgtact ggcgggggtg
      661 gccatcagcc tgctgttcgt ggagctgacc ggcctgtcgc ccgcgggcct gatcgtgccc
      721 ggctacctcg ccctgtgcct tcagacgcct tggaggatcg tctacaccct ggcagtggcc
      781 gtggcggcct ggggcatcgc caaactgctg ggcaatgtga tgatcctgta cggccgccgg
      841 cgctttgcgg tcatggtcct gctctccttt gccatcgatc tggcggtcac cagcttgggc
      901 ctgctggcct atgacccggg aatgatcggg gtgctggtac cgggcatcat ggctcaggag
      961 ctggaacggc agggattttt gaagtctctt gtccctttgg cggcggtggt gggcatcctg
     1021 gcgctgatca tgatgtggca ggggatgcag gtgtttccga tatgagcaga gcgaagcggg
     1081 aacggaagct gctggccctc ctgctggcct gcgcactgct ggcctggggg actgcggcgc
     1141 tggcggcggg gcagggccgc cgaacggaaa cgggctatgc cgctgtgcag ctggcagcgg
     1201 cggatctgct ggaggactgc ttccggcagg tgcgcaccta caaggaggaa ctgggcatcc
     1261 ccctcagtga gctggactac catcagaccg gcatgatcgg ggaaagctac accgggatca
     1321 ccaccaccct gggggccatc gaggcgaagc ggaccaccgc ctggccggaa atgggggcgc
     1381 tgtgtgtccg cctgctccac gaggcgggcg tccgatcggg ggacaccgtg ggggccggct
     1441 tttccggctc cttccccgcc atggatctgg cggtgatcgc cgcctgccag gtgatgggcg
     1501 tggaactgat ctatatctcg tcagtggggg catccaccta cggggccaat catcccgccc
     1561 tcaccttccc ggagatggcc caccggctgg tggaggacgg gctgctggag acagacagcg
     1621 cggccgtcac cctgggcggg ggaggcgatg tgggcggggg gatggacccg gcctgcgcag
     1681 aggagatccg gggtcggctg gcggaggcgg gactgctcct gctggaggag ccggactacc
     1741 ggcgcaatct ggagctgcgc cagcagctct accagcagga ggggcccatc gactgctttg
     1801 tggcagtggg aggcaacgtg acctccatgg ggcgggggga gagcggcatc agcctggggc
     1861 agggcctgat ctccccggag aagatcgtcc ggctgacaga ggacagcggg ctggtgcagc
     1921 gctatctcag ccaggggctt ccggtgatca atcttctgaa catcaagcag ctggcggcgg
     1981 actacggact gccctatgac ccggcccagt ggccggagcg ggggacctct gccgtctact
     2041 ttgagactga ctaccccggg gcggtgctgc tcctgggact ggctggggcc gcggtgctgc
     2101 tggctctgtg ccgatgggtg aggaggagcg cggggaccgc gcggaaggag gagagaggat
     2161 accatggaca gacagaatcg tccgctgacc atcctgacca ttgacgacga ggaacagatc
     2221 cagtacgccc tcagggccct gttcaactcc cagggctggg aggccatctc tgcccgggat
     2281 gtggaggagg gagtgggaaa gttccggacc tgccgcccgg atctggtgct gatggactac
     2341 catctcccca agatcaacgg ggtcaaaggg gtggagatgc tgcgccggga ggacccggag
     2401 gtgccgctga tcgtcttcac catcgaggac aaccagaggg tggccgacca gttcctggat
     2461 gcgggggcga gtgacttcgt gcttaagccg gtgaaggccc tggacatcat cgcccgtatc
     2521 aagctgcacg tgcgtctggt ggagagccga cgggagaacc gccagagcac agaggagccg
     2581 gtgaagggca ttgggcaggg cacactgagc ctgatagagg gttatctgcg cagtgtcagc
     2641 gagtaccagc ctgtggaggc catcgcggaa gggacggggc tggcctatca gaccacctac
     2701 cgatatctgc aatatctggt gtcggtgaat cgggtggagg tgagccagca ctatggaaag
     2761 gtggggcgcc ccaaacagcg ctaccggatg atccgggagg cgggcgacgg ggaaaagccc
     2821 gaaacaggac tataatgcgg gacaaaaagc gccggtcagc cattgggctg gccggcgctt
     2881 tctcattgtc cgggacagaa aaagctgtgg tcaaaataga caaataaaaa tataaaaggg
     2941 aagaggccta aaaaagaggt cggaagagaa aaatgagaaa aaactagaaa taaaagggaa
     3001 aaaatgacgg atcaaaaaag gataagagtg gcctgttcat aaatttttaa aatttcagaa
     3061 ataaataaga gaaaaaattg cacaatgaaa tgggggcgct gtatgataga tttagttaga
     3121 attatggtgg acgcagatgg tcgctgccgt ccgtcacaat ggggggagga ataagctgga
     3181 acagacggaa ataaaacata aggtgaacgc acggccctat tggaaaggag agtcaaaatg
     3241 ggaaacagac gcagacacat tggatcctcg ctggcggccc tgaccctgac cctatccctg
     3301 ctgacgggac tgctcaccgg atgcggcccc ggcgggagcc agagcagttc tccggaggcc
     3361 ggcagcacgc ccccggcggc cagccagagc acgcagacgc cggaacagcc cgccctgccc
     3421 gagctgacca cagataccta cggcaacgcc gcggtgggct atcaggcagc ggtggtcagc
     3481 gccaatgagt acacctcccg catcggcatg gatatcctgg aggcgggggg caacgcggtg
     3541 gacgccgcgg tggccatgat ttttgccaac agcctcaccg agccgggtgc caccagcctg
     3601 ggcggcgcca gcttcatgac catctatctg aaggagacgg gcgagtacat ctgtatcgag
     3661 gccatggaga ccgctcctgc ggcggcgggc atcgacactc tggacgagat caatgagaag
     3721 aagggggcca tgctggtcac cgtccctgga caggtccacg gcgccctgac cgccctggag
     3781 aagtatggca ccatgacccg ggaggaggtg ctggcccctg tcatccagct ggcggaggag
     3841 ggcttcgatg tccacatcag ctttgaggag cgggcctcgg ccagcttcga caaactggtg
     3901 cagaacgagg aggcggccaa gatatttacc aacgaaggcc tgccctatgc ggtgggggac
     3961 cacttcacca accccgacta tgcggatacc ctgcgcaaga tcgcggaggg cggcatcgac
     4021 gagttctaca aggggagcat cgcccagacc atcgtggacg aggtccagcg cctgggcggc
     4081 ctgctcacca tggaggatat ggccagctac accagcgtgg agcgggagcc catcagcacc
     4141 acttatcacg gctatgagat cgtgac
//
LOCUS       DBMDSJ010000106         4159 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5637_length_4159_cov_1.555692, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000106 DBMDSJ010000000
VERSION     DBMDSJ010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5637_length_4159_cov_1.555692"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(336..836)
                     /locus_tag="ACZPAQ_03700"
     CDS             complement(336..836)
                     /locus_tag="ACZPAQ_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6933 domain-containing protein"
                     /protein_id="HIR0903119.1"
                     /translation="MELGLTFPLQRFLGIKTPAWGTEPDRRFCWDLHVIRLRGRGCLL
                     AVHCHSRYAFVRWDVPPLQREDIPGLFRAGLTDSLTAAGLSPARVEGYLEKAGPACLT
                     RTHGRREVAFLNRAWEDVLALDLCLDMERQAQPLLDRAVNTCSSRCAGFEGLGTGLER
                     ISASLL"
     gene            complement(853..1302)
                     /locus_tag="ACZPAQ_03705"
     CDS             complement(853..1302)
                     /locus_tag="ACZPAQ_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR0903120.1"
                     /translation="MRTAVCYYSRHHGNTRKVLEAMAREGEIDLIDATVCQTVRLEDY
                     DCVGFASGVYFGKFHTSVLHAAQQYLPHGKPVFFVCTYGGGMGQSTRELKELAGERGC
                     AVLGTFGCKGYDTFGPFKLVGGLAKGRPDEGDLDRARSFFRDILTRL"
     gene            complement(1318..1728)
                     /locus_tag="ACZPAQ_03710"
     CDS             complement(1318..1728)
                     /locus_tag="ACZPAQ_03710"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR0903121.1"
                     /translation="MIRDICKDETFLAQKAELATPEDLPAAADLLETLTHHSAGCVGM
                     AANMIGVNKRIIAFDNEGSYLVMLNPEIVKRSGPYEAEEGCLSLSGTRRARRWRSIKV
                     RWQNERFQERCKTFTGWTAQIIQHELDHCEGILI"
     gene            complement(1807..2112)
                     /locus_tag="ACZPAQ_03715"
     CDS             complement(1807..2112)
                     /locus_tag="ACZPAQ_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903122.1"
                     /translation="MDAEKGSFVPVAFFSFLCYNACDSLKEEARITAIENGERITRGL
                     EDGPRRVLNWLELDPWPDEIRSLPLFQNEAAGFSGEDQHSFELKRPAGSGPERGNGL"
     gene            complement(2153..2560)
                     /locus_tag="ACZPAQ_03720"
     CDS             complement(2153..2560)
                     /locus_tag="ACZPAQ_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HIR0903123.1"
                     /translation="MLKGKLIHPDIMAALALCGHGDKVLIADGNYPLDSKTGQAETVY
                     LGLTPGLPTVTDVLAAIQSTVNIEKAEVMDPADGTTPEIFGEFQTMLDGMELNKLGRY
                     EFYDACCQPGVRLAISTGEQRIFSNLLITIGVA"
     gene            complement(2612..3226)
                     /locus_tag="ACZPAQ_03725"
     CDS             complement(2612..3226)
                     /locus_tag="ACZPAQ_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR0903124.1"
                     /translation="MLKPESKQAVSNFFAAAETEFNAFLKARSPEEYEAAAQLILDSQ
                     AKGGRLHVTGIGKCSHVSTYAASLFSSTGNPAYYLHGTEAVHGSCGQLAAGDVVIAIS
                     NSGETAELKATVLAVQNNGCKVIGVSGNPNSWLAKHTDAFLFAGVKAEGGPLNRAPRN
                     SVLAELLTLQALSVALQVEKDWDPVKYVRCHPGGKLGELRENEK"
     gene            complement(3290..>4159)
                     /locus_tag="ACZPAQ_03730"
     CDS             complement(3290..>4159)
                     /locus_tag="ACZPAQ_03730"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR0903125.1"
                     /translation="GANQAVQCARLGAHVTMVGQVGDDAFGKIMTDTAAAAGVDVSHV
                     SVDPQESSGVGHITLEVTEHGAQNRITVCPGANFTITVDDVAWLREEIGRFDLVMMQF
                     ELPMEVIEAVAQWAHDAGVPVMINPAPAAPMSDRLLACATYLSPNEHEAAILAGHSID
                     VSNGINMEDVAVVSQAFQARGVENLIITMGENGSIAAGKNGIHHTHCVKMEHVADPTA
                     AGDSFVAAFCTGLTAGLPQGEALAFASHTAAITVSRMGAMPSLPTVEEVQQLLVERDY
                     QGFDPAELDALKQ"
BASE COUNT          712 a         1302 c         1397 g          748 t
ORIGIN      
        1 gcggcggagg cggcggacaa ttccagcacg gtggtctgct tctcctccaa ggctgccaga
       61 gtagagccgt gaagatccgg ggacgaggcg ggaccggaaa ggacgaacac ggacagcagt
      121 gcgatgagca gcggaatgac gacaaacagc gtcttttttt gcagaacttc cttcatagct
      181 tttctctctt tccatttctt ttttgcatga aaaaatggcg ggcagtgcaa ggcggcgcgc
      241 tcaacagtta cctctgtggc ctgccgtctt cacaacagcc tttgcagagc tcgcgaagca
      301 gaggagaaaa ctagggggga tgccctgcgt cccgctcata acagcgatgc ggatatccgc
      361 tccagccctg tccccagtcc ctcaaagcct gcgcaccggc tggagcaggt gttgacggca
      421 cggtccagca ggggctgggc ctggcgttcc atgtccaggc acaggtccag cgccagcacg
      481 tcctcccacg cccggttgag gaaggccacc tcccggcggc catgagtgcg ggtgaggcag
      541 gcgggcccgg ccttttccag atagccctcc acccgggccg gggagaggcc ggcggcggtg
      601 aggctgtcgg tcaggcctgc gcggaagagg ccggggatgt cctcccgctg gagagggggc
      661 acatcccagc ggacaaaggc gtagcggctg tggcagtgga cggccagcag gcacccgcgg
      721 ccccgcaggc ggatgacatg caggtcccag cagaagcgcc ggtccggctc tgtgccccag
      781 gccggcgttt ttatccccag gaaccgctgg aggggaaagg tcagtcccag ttccatcacg
      841 ccgcctcctc tctcacaggc gggtcaggat gtcccggaag aagctgcggg cgcggtccag
      901 gtccccctca tccggccggc ccttggccag gccgcccacc agcttgaagg ggccgaaggt
      961 gtcgtagccc ttgcagccga aggtccccag cacggcgcag ccccgctccc cggccagctc
     1021 cttcagctcc cgggtgctct gtcccatgcc gcccccgtag gtgcagacga agaacacagg
     1081 cttgccatgg ggcagatact gctgggcggc gtgtaagacg ctggtgtgga acttcccaaa
     1141 gtagacgccg gaggcgaagc cgacgcagtc gtagtcctcc agccggacgg tctggcagac
     1201 ggtggcgtcg atcaggtcga tctccccctc ccgggccatg gcctccagca ccttccgggt
     1261 gttgccgtgg tggcgggaat agtaacagac ggcggttctc atgacatgac gctcctttta
     1321 aatgaggatg ccctcgcagt ggtccagctc gtgctggatg atctgggcgg tccagccggt
     1381 gaaggtctta caccgctcct ggaaacgctc gttctgccag cggaccttga tggaccgcca
     1441 ccgccgggcc ctccgggtgc cggagaggga caggcagccc tcctccgcct cataggggcc
     1501 ggaccttttg acgatctccg ggttgagcat gaccagatag ctgccctcgt tgtcaaaggc
     1561 gatgatgcgc ttgttcacgc cgatcatatt ggccgccatg cccacgcagc ccgcgctgtg
     1621 gtgggtcagc gtctccagca ggtccgctgc cgcgggcagg tcctccggcg tagccagctc
     1681 ggccttctgg gccagaaagg tctcgtcttt gcagatgtca cggatcataa tttcgatctc
     1741 tccttttgcg gcaggctcac cccggcacag cgcgcagcgc ctgtctggag gtcggtgacc
     1801 gtcccctcaa agtccgtttc ccctctccgg gccggacccg gctgggcgct tgagctcgaa
     1861 ggagtgctga tcctccccgg agaagccggc ggcctcgttt tggaagaggg gcagagatct
     1921 gatctcgtcg ggccatgggt ccagctccag ccagttgaga acgcggcggg ggccatcctc
     1981 cagtccccgc gtgatcctct cgccgttctc aatggctgtg atacgcgcct cctctttcag
     2041 gctgtcacag gcattatagc acagaaatga gaaaaatgcc acgggaacaa aggagccttt
     2101 ctccgcatcc atgcggagaa aggctcccta aaaacgagtg agacaggccg gatcaggcca
     2161 cgccgatggt gatcagcagg ttggaaaaga tacgctgttc cccggtggag atggccagcc
     2221 ggacgccggg ctgacagcag gcgtcataga actcatagcg tcccagcttg ttcagctcca
     2281 tgccgtccag catggtctgg aactccccga agatctccgg agtggtgccg tcggcggggt
     2341 ccatgacctc cgccttctcg atattcaccg tgctctggat ggcggccaga acgtcggtaa
     2401 cagtgggcag gccgggggtc agccccaggt acacggtctc tgcctggccg gtcttggagt
     2461 ccagggggta gttgccgtct gcgatgagga ccttgtcccc gtggccgcac agggccaggg
     2521 cggccatgat gtcggggtgg atgagctttc ctttcagcat ttgaatgacc tcactttttc
     2581 tccggagggg acggggcccg ccccggaacg tttacttttc gttctcccgc agctcgccca
     2641 gctttccgcc ggggtggcag cggacgtatt tgacggggtc ccagtccttc tccacctgga
     2701 gcgccacaga gagggcctga agggtgagca gctccgccag cacggagttg cggggggccc
     2761 ggttcagggg gccgccctcc gccttgacgc cggcgaagag gaaggcgtcg gtgtgcttgg
     2821 caagccagct gttcgggttg ccggagacgc cgatgacctt gcagccgttg ttctggacag
     2881 ccagcacggt ggccttcagc tcggcggtct caccggagtt ggagatggcg atgaccacgt
     2941 ccccggccgc cagctgcccg caggagccgt ggacggcctc ggtgccgtgg aggtagtagg
     3001 cggggttgcc ggtggaggag aacagggagg cggcgtaggt ggacacatgg ctgcacttgc
     3061 cgatgccggt gacgtggaga cggccgccct tggcctggga atccaggatc agctgggcgg
     3121 cggcctcgta ctcctcggga ctgcgggcct tcaaaaaggc attgaattcg gtctctgcgg
     3181 cggcaaagaa attggatacc gcctgtttgc tctcaggttt cagcataacg actctcctta
     3241 tggataggat ttgactgccc ccgcggggac ggggacagtc aaggacagat cactgcttca
     3301 aagcgtccag ctccgccggg tcaaagccct ggtagtcccg ctccacaagg agctgctgga
     3361 cctcctccac cgtgggcagg ctgggcatgg cgcccatacg ggacacggtg atggcggcgg
     3421 tgtggctggc aaaagccagg gcctcgccct ggggcagacc ggcggtgagg ccggtgcaga
     3481 aggcggccac aaaggagtcg ccggcggccg tggggtcggc cacatgctcc atcttcacgc
     3541 agtgggtgtg atggatgccg tttttccccg ccgcgatgga gccgttttcc cccatggtga
     3601 tgatgaggtt ctccaccccc cgggcctgga aggcctggga gaccacggcc acgtcctcca
     3661 tgttgatgcc gttggagacg tcgatggagt ggccggccag gatggcggcc tcgtgctcgt
     3721 tgggggagag gtaggtggcg caggcgagca gccggtcgga catgggggcg gctggggcgg
     3781 ggttgatcat cacggggacc ccggcgtcgt gggcccactg ggccaccgcc tcgatgacct
     3841 ccataggcag ctcgaactgc atcatcacca ggtcaaagcg gccgatctcc tcccggagcc
     3901 aggccacgtc gtccaccgtg atggtgaagt tggcgccggg acagacagtg atgcggttct
     3961 gggcgccgtg ttccgtcacc tccagggtga tgtggcctac gccggaggac tcctgggggt
     4021 ccactgacac atgggacacg tccacaccgg cagcggcagc ggtatccgtc atgatcttac
     4081 cgaacgcatc gtcccccacc tggcccacca tggtcacgtg ggcgcccagg cgggcacact
     4141 ggaccgcctg attggcgcc
//
LOCUS       DBMDSJ010000107         4157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5641_length_4157_cov_1.611933, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000107 DBMDSJ010000000
VERSION     DBMDSJ010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5641_length_4157_cov_1.611933"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(131..388)
                     /locus_tag="ACZPAQ_03735"
     CDS             complement(131..388)
                     /locus_tag="ACZPAQ_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HIR0903126.1"
                     /translation="MNDQERITDFLNSEKKMSLNYGIFASECVSVPLRDEFLKLINQS
                     HQTQTQLFTTAQSKGWYSPEQAPQDKISQAYTKYSNQQPTV"
     gene            complement(402..587)
                     /locus_tag="ACZPAQ_03740"
     CDS             complement(402..587)
                     /locus_tag="ACZPAQ_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HIR0903127.1"
                     /translation="MPILTTKELQALNDQLDFERVLHCKYLAAVQESTDQELKTCFQN
                     LAGQHQQNYTCLLKYLH"
     gene            complement(608..1189)
                     /locus_tag="ACZPAQ_03745"
     CDS             complement(608..1189)
                     /locus_tag="ACZPAQ_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903128.1"
                     /translation="MVEKIMLEGGGTLSLREDGPRVHTEARRQNDGQGLYKVWLRGGG
                     KGRLLLGTLVPEGGELVLSRTLSRRTLEDAGCWPVAGGRAVMAYPFTEKSAGEWYWEE
                     EPWKLVSDPVLVQAARSWGPLLVQRGGEEVRLAASFRGGGQFPLVPLFCFGTVGQIRG
                     REYVIFLFDRQGTPKMPYNLEKGGKPKAVDQSR"
     gene            complement(1295..1924)
                     /locus_tag="ACZPAQ_03750"
     CDS             complement(1295..1924)
                     /locus_tag="ACZPAQ_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903129.1"
                     /translation="MRRIGAWMMALLLCVVGTGCGRQLGSPISYEDFTGTYPVEGYVP
                     SQEDLGRAPYSYAGAGPHFSVVLNVRQASEAERSVLIQGKWASAETMAQSGADFPERS
                     EEYNALALKATEEAMALEEAEQIYLTELLVTHNGTEAEQFRYSVSDGGTLYLSGYAAG
                     GEVWCRLANTPDGSYTEGLLLPFRQQYSMEICYGEEREEIALTLQEQAG"
     misc_binding    2089..2329
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2388..4079
                     /gene="leuA"
                     /locus_tag="ACZPAQ_03755"
     CDS             2388..4079
                     /gene="leuA"
                     /locus_tag="ACZPAQ_03755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR0903130.1"
                     /translation="MKPGYQKYIPFQPQPIQGRTWPDRVITQAPVWCSVDLRDGNQAL
                     VDPMNLEEKLEYFHTLVDIGVKEIEIGFPSASETEYEICRELIEGGHIPDDVTIQVLV
                     QAREHLIRKTFEAIQGAKNVILHFYNSTSTLQRKVVFHMDTDEITKIATDAADLIYEM
                     SQPLIQSGMNLRFEYSPESFMGTEMDYAVEVCQAVLEHLHAAPERKVILNLPTTVENC
                     MPNQFADMLEYFIRRLPSRDCAIISLHPHNDRGTGVATTEMGLLAGAERVEATLFGNG
                     ERTGNVDMITLALNMYTQGVDPRLDFSNINKIREMYERCTKMKVGERQPYAGELVFTA
                     FSGSHQDAINKGTQYMKESNSDFWEIPYLPIDPADVGRQYEPIIRINSQSGKGGSAYV
                     MEHNFGFDLPKLMHPEFGAVVQKETDRVGTEIAPERIYELFKEEYLNIDAPYRMLKHA
                     FTESIDAEGHSHVTFWGTIQHTDTIFQVSGEGNGPIDAFFNAIKDEKMSHFTFLDYKS
                     HAITDGSDSQGVAYILLQDQRDGRKYWGVGVSHNINLAPLRGILSAINRSKRSAQ"
BASE COUNT          789 a         1427 c         1114 g          827 t
ORIGIN      
        1 ccacagcagc atcctccgct ggtgatatta ttgtaaacct agacagcccg tttgtcaatc
       61 cttttttgca ggatttttct ggtcaatctg caagagggag ggactgtgct tagtccctcc
      121 ctccgcttcc ttatacggtg ggctgttggt ttgaatactt ggtgtaagct tggctaatct
      181 tatcctgggg cgcctgttcg gggctgtacc agcccttgct ctgggctgtg gtgaacagct
      241 gggtctgggt ctggtggctc tggttaatca gcttgaggaa ctcatcccgc aaggggacgc
      301 tcacgcactc agaggcaaaa ataccgtagt tcagggacat tttcttctca ctattcagaa
      361 aatctgtaat gcgttcctgg tcgttcatgc tctcgtcctc cttagtgcag atacttcagc
      421 aggcaggtgt aattctgctg gtgctggcca gccaggttct ggaagcaggt cttcagctcc
      481 tggtcggtgc tctcctggac cgcggccaga tacttgcagt ggagcacccg ctcaaagtcc
      541 agctggtcat tgagggcctg gagctccttg gtggtcaaaa tgggcatacg aattcctcct
      601 tcagccgtca tcggctctga tctacggcct taggtttccc gcctttttcc aaattatacg
      661 gcattttagg ggtcccctgc cgatcaaaca gaaagatcac atattccctt ccccggatct
      721 ggcccaccgt cccgaagcag aagaggggga ccagcgggaa ctgcccgccg ccgcggaagg
      781 aggcggccag ccgcacctcc tccccgcccc gctgtaccag caggggcccc cagctccggg
      841 ccgcctgcac cagcactggg tcggacacca gcttccaggg ctcctcctcc cagtaccact
      901 ccccggcgct cttctctgta aaaggatagg ccatcacagc cctccctccc gccaccggcc
      961 agcagcccgc atcctccaga gtacggcggc tcagggtccg gctcagcacc agctctcccc
     1021 cctccggcac cagcgttccc agcagcagcc gtccctttcc gccgccccgc agccacacct
     1081 tatagagccc ctggccgtcg ttctggcggc gcgcctcggt gtgcaccctg gggccgtcct
     1141 cccgcaggga gagggtcccg cctccctcca gcatgatctt ttccaccacg gcctgtccct
     1201 ccctctcccc gtctcgctcc actttatgca ccccgggggc gtggatatgc cgctgtggga
     1261 aaggcgccgg ccgcatgccc gcggccggcg cctttcatcc cgcctgctcc tgaagggtga
     1321 gggcgatctc ctcccgttcc tccccataac agatctccat gctgtactgc tgacggaagg
     1381 ggagcaggag cccctcggta tagctcccgt ccggcgtgtt ggccagccgg caccagacct
     1441 ctccgcccgc cgcataccca gacagataaa gcgtcccgcc gtcagagacg gagtagcgga
     1501 actgctctgc ttccgttccg ttatgggtca ccagcagctc cgtcagatag atctgctccg
     1561 cctcctccag cgccatggcc tcctccgtcg ccttcagagc cagagcgttg tactcctcac
     1621 tgcgctccgg gaaatccgcc ccgctctgag ccatggtctc tgctgaggcc cactttccct
     1681 gtatgagcac ggaccgttcc gcctcgctgg cctgtctgac gttcaggacc acagaaaaat
     1741 gcggacctgc tccggcatag gaatagggtg cccgccccag gtcctcctgg cttgggacat
     1801 agccctccac gggataggtg cccgtaaaat cctcatagga tatcgggctc ccgagctgcc
     1861 tcccgcatcc tgtcccgacg acgcacagca ggagcgccat catccacgca ccgatccgtc
     1921 tcatcccttt cccgcctcct tgtcccctca attgtaccac aagccgaatc aatatcccat
     1981 ttatgttttt cccgctccat taagattttt taatttgtcc ccgccttttt caggtctgtc
     2041 tcatttttct cttgacaaag ccggcctcct ccgctaaaat aacattcaac aaatgcagtg
     2101 acgggaaaaa agacagggtc agcccccttc agagagccgg tggttggtgg gaaccggcgt
     2161 gcggccccct gtggatactg gtccccgagc agcttgtccg aatctgtgag taggatgaga
     2221 cgtatggccc cgttaccggc ctcagccttg ttcgaggctt atgagggccg ctttcgggta
     2281 accggcggcc gaaccagggt ggtaccgcgt catttccgac gcccctgact tgaaaaagtc
     2341 gggggcgtcg tttttgtccc ccgcagaatg taaaggaggc accccatatg aaacccggat
     2401 atcaaaagta catccccttc cagccccagc ccatccaggg ccgcacctgg ccggaccgcg
     2461 tcatcaccca ggcccccgtc tggtgttctg tggacctgcg ggacggcaac caggctctgg
     2521 tggaccccat gaacctggag gagaagctgg aatattttca caccctggtg gacatcggcg
     2581 tcaaggagat cgagatcggc tttccctccg ccagtgagac cgagtatgag atctgccgcg
     2641 agctgatcga ggggggccac atccctgacg atgttaccat ccaggtgctg gtccaggccc
     2701 gggagcacct gatccgcaag accttcgagg ccatccaggg cgccaaaaac gtcatcctcc
     2761 acttctacaa ctccacctcc accctccagc gcaaggtggt cttccacatg gacaccgatg
     2821 agatcaccaa gatcgccacc gatgccgccg acctgatcta cgagatgtcc cagcccctga
     2881 tccagtcggg tatgaacctc cggttcgagt actcccccga gtccttcatg ggcacggaga
     2941 tggactatgc cgtggaggtc tgccaggcgg tgctggagca cctccacgcc gccccggagc
     3001 gtaaggtcat cctcaacctg cccaccacgg tggagaactg catgcccaac cagtttgccg
     3061 acatgctgga gtatttcatc cgcaggctcc cctcccggga ctgcgccatc atctccctcc
     3121 acccccataa tgaccggggc accggcgtag ccaccaccga gatgggactg ctggccgggg
     3181 cggagcgggt ggaggccacc ctcttcggca acggtgagcg caccggcaac gtggatatga
     3241 tcaccctggc tctgaatatg tacacccagg gcgtggaccc caggctggac ttttccaata
     3301 tcaacaagat ccgggagatg tacgagcgct gcaccaagat gaaggtgggc gagcggcagc
     3361 cctatgccgg cgagctggtc ttcaccgcct tctccggcag ccaccaggac gccatcaaca
     3421 agggcaccca gtatatgaag gagtccaact ccgacttctg ggagatcccc tacctgccca
     3481 tcgaccccgc cgacgtgggc cggcagtatg aacccatcat ccgcatcaac agccagtcgg
     3541 gcaagggcgg ttccgcctat gtgatggagc acaacttcgg cttcgacctg cccaagctga
     3601 tgcaccccga gttcggcgcc gtcgtccaga aggagaccga ccgggtgggc accgagatcg
     3661 cccccgagcg catctacgag ctgttcaagg aggagtacct gaacattgac gccccctacc
     3721 ggatgctcaa gcacgccttt accgagagca tcgacgccga ggggcactcc catgtcacct
     3781 tctggggcac catccagcac acggacacga tcttccaggt ctccggcgag ggcaatggcc
     3841 ccatcgacgc cttcttcaac gccatcaagg acgagaagat gtcccacttc accttcctgg
     3901 attacaagtc ccacgccatc accgacggct ccgactccca gggcgtggcc tacatcctgc
     3961 tccaggacca gcgggacggc aggaagtact ggggcgtggg cgtctcccat aacattaacc
     4021 tggcccccct gcgcggcatc ctctctgcca tcaaccgctc caagcggagc gctcagtgac
     4081 ggtcgctccc acgccatccc ccatgctccg gcccgaaact gtggaaaaag cggcagtctg
     4141 ccgcgcgcag ggggtga
//
LOCUS       DBMDSJ010000108         4146 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5656_length_4146_cov_1.487515, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000108 DBMDSJ010000000
VERSION     DBMDSJ010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_4146_cov_1.487515"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..485
                     /locus_tag="ACZPAQ_03760"
     CDS             60..485
                     /locus_tag="ACZPAQ_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903131.1"
                     /translation="MKIYNKKKFADGCFFLVLGAALAGAIIWKREIDGKSVVLALIAL
                     VIGADALVRSLSRRLSYQDKVEALDERNIQVELRARSRTLSLLRGTLMAVLILSLIGA
                     LANPKGELVYGGMMVVSGVLYTLSLFIELACSFYYEGRI"
     gene            535..1269
                     /locus_tag="ACZPAQ_03765"
     CDS             535..1269
                     /locus_tag="ACZPAQ_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903132.1"
                     /translation="MSHTREEEWKQVRALHLVRDERDREVGAAAKGRAGDAVLAASQL
                     FAAVCLLRGDPAWAAFLSLTFVGGGAQCLHRFWEDRERFFLICGLAASACGAALGCWY
                     LLQDPGTGPSLGRLAAFALLISLLTSLSGLIFVPLLLGAVWLKYKVGRMDGEAWEHYF
                     QSVSTVGLLLRGGVLLLLALGLTALLSWPLFALLGFPMPERLALLFFLIGGADLFRKL
                     SQERDELVRKLLRLKLPGGRKGVTEQ"
     gene            1356..1769
                     /locus_tag="ACZPAQ_03770"
     CDS             1356..1769
                     /locus_tag="ACZPAQ_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HIR0903133.1"
                     /translation="MKVKNIRHLMLGIICLVLAVICLAVGAAGGSAPKYLISAVLLLA
                     LGAVNFHGALKGGAEQELLRWTDERDRLVAMRSCQITLQVMNGLLFGGAMTALLCYAI
                     TRRALFQTVGITLCAVILTLFFVTMAANRYFERRM"
     gene            complement(1886..2515)
                     /locus_tag="ACZPAQ_03775"
     CDS             complement(1886..2515)
                     /locus_tag="ACZPAQ_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0903134.1"
                     /translation="MRGIRRIPCGNVNCYLVQGEEGAVLVDTGYTGYVERLLDTCLGA
                     GVRLIVLTHGHLDHIQNTALLMERLGVPAALHRADLGLLTDQQSQPLSAQGPAGRLLL
                     SLSRHSFQTQAFLPFTPTVELREGDSLAPWGVDARVLELPGHTAGSIGLDVEGHSLIV
                     GDAMMNLFRPGPSCLCSDPAAQRDSIARIRSLGPRSLYFGHGRPAHLPG"
     gene            complement(2512..3804)
                     /gene="mtaB"
                     /locus_tag="ACZPAQ_03780"
     CDS             complement(2512..3804)
                     /gene="mtaB"
                     /locus_tag="ACZPAQ_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR0903135.1"
                     /translation="MRIAFYTLGCKVNQYETQALEQIVTARGHLLVPFEGEADAYIIN
                     TCTVTAVSDKKSRQVIRRARKRAPDAVIAVCGCYPQTHPAELEGLSIDLVSGTGDRLG
                     FAGLLERTWQERQPVTALDNAFDRRSFEILPAGGLEGRTRAMLKIEDGCVNFCSYCII
                     PYARGRVRSLSPQDTRRELERLLEAGYREIVLTGIEISSWGQDLPGEQTLIDLLETVC
                     RDLPGEVRIRLGSLEPRTITPDFCRRAAALPNLCPHFHLSMQSGCDAVLARMNRKYDS
                     ARYYESVQLLHGYFDRPAITTDLIVGFPGETEEEFQQTLTFLHRCAFAAMHIFPYSKR
                     PDTPAAKMPGQVLNAVKEERARRAAETAGEMEHTYLSQWVGQTVPVLFEEEQGGLWRG
                     HTAHYCAVAAPSPRDLHNCLVQVQLEGLEGNTLKGVVL"
     gene            complement(3801..>4146)
                     /locus_tag="ACZPAQ_03785"
     CDS             complement(3801..>4146)
                     /locus_tag="ACZPAQ_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013234.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR0903136.1"
                     /translation="GKIMLIEFEKLQENLVPKPRGGEGEMYTRTFQDENNKIMQIRLT
                     PGSHLGMHTHEVDSEAFYILSGTGKVLCDGVDEPLAPGSFHYCPKGHGHRLMNDGTED
                     LVLVAIIPNHGA"
BASE COUNT          709 a         1221 c         1450 g          766 t
ORIGIN      
        1 cgtgacggtg gaggacctgt actgcctgaa agaaaatagg gaattggagg accggcagta
       61 tgaagatcta caataagaaa aagtttgcgg acggatgttt tttcctggtc cttggagcgg
      121 ccctggctgg ggccataatc tggaagcggg agatagatgg aaagagtgtg gtgcttgccc
      181 tcatagccct ggtcatcggg gcagacgcat tggtccgcag cctctccaga cgcctgtctt
      241 accaggacaa ggtggaggca ctggacgagc gcaacatcca ggtggagctg cgggccagga
      301 gccggacgct ctccctgctg cggggcaccc tgatggcggt gctgatcctc agcctgatcg
      361 gcgccctggc caacccgaag ggcgagctgg tgtacggcgg gatgatggtc gtatccggcg
      421 tgctgtacac cctctccctg ttcattgagc tggcatgcag cttctattat gaaggcagga
      481 tatagggatc ggatcacgga agatccggat gaaaaggaaa gcgaggaatt ccatatgagc
      541 catacccgtg aagaggagtg gaagcaggtc cgggcgctcc acctggtccg ggacgagcgg
      601 gaccgggagg tgggggccgc ggccaaaggc cgggcggggg atgcggtgct ggctgcctcc
      661 cagctgtttg cggcggtctg tctcctgagg ggggacccgg cctgggccgc cttcctgtcc
      721 ctcaccttcg tggggggcgg ggcgcagtgc cttcatcggt tctgggagga tagggagagg
      781 tttttcctga tctgcggcct ggccgcgtcg gcctgcggcg cggcgctggg atgctggtat
      841 ctgctccagg acccggggac agggccgtcc ctggggcggc tggcggcctt tgccctgctg
      901 atcagcctgc tgaccagcct gtccggcctg atatttgtcc ctcttctgct gggggccgta
      961 tggctcaagt ataaggtggg ccgtatggat ggggaggcgt gggagcacta tttccagtcc
     1021 gtgtccacgg tggggctcct gctgcggggc ggcgtgctgc tgctgttggc cctggggctg
     1081 accgccctgc tgagctggcc gctgttcgcc ctgctgggct tccccatgcc ggagcggctg
     1141 gccctgctgt tctttttgat cgggggcgcg gacctgttcc gcaagctgag ccaggagcgg
     1201 gacgagctgg tgcggaagct gctccggctg aagctccccg gggggcggaa gggcgtcacg
     1261 gagcagtgag tgcccctccg gacggaaaag agggccgtat ggggcctgcg gggcgctctg
     1321 gcccgatctg ttaagcggga aaggaaggga tcgaattgaa ggtcaaaaac atccggcacc
     1381 tgatgctggg gatcatctgc ctggtgctgg cggtgatctg tctcgcggtg ggggcggcag
     1441 gcggatctgc gcccaaatat ctcatcagcg ccgtcctgct gctggctctg ggcgctgtga
     1501 actttcacgg cgcgctgaag ggcggggcgg agcaggagct gctgcgctgg acggacgagc
     1561 gggaccggct ggtagccatg cggagctgcc agatcaccct gcaggtgatg aacggcctgc
     1621 tgttcggcgg cgcaatgacc gcgctgctct gctacgccat cacccgcagg gccctgttcc
     1681 agacagtggg catcacgctg tgtgccgtga tccttacact gttttttgtg acgatggcgg
     1741 ccaaccgcta ctttgagcgg cggatgtaag atgggaaaag tcccggtgca ttttctgcac
     1801 cgggactttt cagcttgtag aaaaagcctc gcagagttcg cgcccgcgcg cggcagactg
     1861 cgatattctc gaaaaggcgg tttccttatc cagggaggtg agctggacgg ccatggccga
     1921 aatagaggga tctgggaccg agagagcgga tgcgggcgat gctatcccgc tgggcggcgg
     1981 ggtcggagca gaggcaggag gggcccgggc ggaacaggtt catcatggcg tcccccacaa
     2041 tgagggagtg cccctccacg tccaagccga tggagcccgc cgtatggccg ggcagctcca
     2101 gaacccgggc gtccacaccc cagggggcca ggctgtcccc ttcccgcagc tccactgtgg
     2161 gtgtaaaagg gaggaacgcc tgggtctgga agctgtgacg ggagagagac agcaggagcc
     2221 ggcccgccgg tccctgggcg gacaggggct gagattgctg atcggtgagc aggcccagat
     2281 ccgcccgatg gagggcggcg ggaacgccca gccgctccat aagcagggcg gtgttctgaa
     2341 tgtggtccaa gtggccgtgg gtcagcacga tcagccgcac cccggccccc aagcaggtat
     2401 ccagcaggcg ctccacgtag ccggtgtagc cggtgtccac caggacggcg ccctcctccc
     2461 cctgcaccag atagcagttc acattgccgc aggggatgcg gcggatgccc ctcacaggac
     2521 cacccccttg agggtgttcc cctccagccc ctccagctgt acctgcacca ggcagttgtg
     2581 aagatcccgg ggactgggag cggccacagc gcagtagtgg gcggtatggc cccgccagag
     2641 accgccctgc tcctcctcga aaagcaccgg aacggtctgc cccacccact gggacagata
     2701 ggtgtgctcc atctctccgg ctgtctcagc ggcccggcgg gcccgctctt ccttcacggc
     2761 gttgaggacc tggccgggca ttttggccgc cggagtgtcg gggcgcttgg agtagggaaa
     2821 gatatgcatg gcggcgaagg cacacctatg gaggaatgtc agggtctgtt ggaactcctc
     2881 ctccgtctca ccggggaagc ccacgatcag gtctgtggta atggcggggc ggtcaaagta
     2941 tccatggagc aattggacgc tctcataata ccgcgcggaa tcatatttcc gattcatccg
     3001 ggccagaacg gcgtcacagc cggactgcat ggacagatgg aagtgggggc acagattggg
     3061 cagggcggct gcccggcggc agaagtcagg ggtgatggtg cggggctcca ggctgcccag
     3121 gcggatgcgg acctctccgg gcaggtcccg gcagacggtt tccagcaggt cgataagggt
     3181 ctgctcccct ggcaggtcct gtccccagga ggagatctca atgccggtga ggacgatctc
     3241 ccgatacccc gcctccagca gccgctccag ctcccgccgg gtgtcctggg gggagaggga
     3301 gcgcacccgc ccccgggcgt aagggatgat gcagtaggag cagaagttga cgcagccgtc
     3361 ctcgatcttc agcatggccc gagtgcggcc ctccaggccg ccggcgggca ggatctcaaa
     3421 ggagcggcgg tcgaaggcgt tgtccagggc ggtgacgggc tggcgctcct gccaggtgcg
     3481 ctccagcaga ccggcgaagc ccagccggtc cccggtgccg gacaccaggt caatggacag
     3541 cccctccagc tccgccggat gcgtctgggg atagcagccg cacacggcaa tgacggcgtc
     3601 cggggcgcgc tttctggccc ggcggatcac ctgtctggac tttttgtcac tcaccgcggt
     3661 gaccgtgcag gtgttgatga tgtaggcgtc tgcctctccc tcaaagggca ccagaagatg
     3721 gccacgggca gtcacgatct gctccagggc ctgtgtctcg tactggttga ccttgcatcc
     3781 aagggtgtaa aaggcgattc tcatgcgccg tggttgggaa tgatggccac caggaccagg
     3841 tcctcggtgc cgtcattcat caggcggtgg ccatggccct tggggcagta atgaaagctg
     3901 ccgggagcca gcggctcgtc cactccgtca cacagtacct ttccggtccc ggacaggata
     3961 tagaacgcct cgctgtccac ctcatgggtg tgcatcccca ggtggctgcc gggggtcagg
     4021 cggatctgca tgatcttgtt attttcgtcc tggaaggtcc gggtgtacat ctccccctcc
     4081 ccgcctcgag gcttggggac caggttctcc tgcagctttt caaactctat gagcataatt
     4141 tttcct
//
LOCUS       DBMDSJ010000109         4126 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5682_length_4126_cov_1.412174, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000109 DBMDSJ010000000
VERSION     DBMDSJ010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5682_length_4126_cov_1.412174"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="ACZPAQ_03790"
     CDS             <1..1507
                     /locus_tag="ACZPAQ_03790"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR0903137.1"
                     /translation="IQGLYPEEETPAFEGRVIDGGGRYLAPGFIDIHTHSDACPLVDY
                     EPESKLHQGITTEITGNCGISILPSTPESRDEIGRYFFSQLELPAGSLPQLGVSGLEE
                     YARMVADHGCAANYGQLVGHGTLRGAVMGFVDRDPEPEEMEQLKDLLERELKAGAFGM
                     SLGLIYPPSAFCKSEELVELAKVLKKYDALLTVHMRNEGPRIFQAVDEMIDITRRSGV
                     HLQISHLKLMGKPQWGRSQELLDKIEAARQEGMTITCDQYPYTATSTSMTALLPKWAH
                     DGGVSALVARVKAPDQRLKDETAAEMEDRGGPAHVMVSGTHGYHPEWEGRTVAELAEE
                     LGLSPVDTVMHVLSACEGSVACIYFCINEDDMLNIMRDMRIALGSDGYGFSYDRSITR
                     TNPHPRSFGTFPRFLELARDHRLMPLEDAVYKITGLPADILGLKDRGTLEEGRIADIT
                     VFDPEQVRDLSEYTDSVKKPAGIRHVLVGGTFALKDGAATGSRTGTVLKKV"
     gene            1638..3260
                     /locus_tag="ACZPAQ_03795"
     CDS             1638..3260
                     /locus_tag="ACZPAQ_03795"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR0903138.1"
                     /translation="MYDLILRGGTVVDGTRSRPYAADVCIRDGRIARIAPDAGEEARE
                     VVDVSGLTVAPGFIDIHSHSDACPLLSFQPESKLFQGITTEITGNCGTSILPSLPENS
                     REIVQYFFDDTSMFSQVVLNEKDRSLDGLYGVEEYARAVAAHGCTANYGQLVGHGTLR
                     GAVMGFVDRDPEPEEMERLKDLLERELRAGAFGMSLGLIYPPSAFCKREELVELAKVL
                     KKYDALLTVHMRNEGPRIFQAVDEMLDITRRSGVHLQISHLKLMGRPQWGRSRELLDK
                     LAAARREGLNVTCDQYPYTATSTSMTALVPKWAHDGGRAALLERLRDPSRQLKAEITA
                     EMEDRGGADRVMIAGTHGCRPEWEGRTVAELAEELKLSPADTVVHALDCCGGRVACIY
                     FSIDEGDMLNIMKDMDIAVGSDGYGLSFDRSITITDPHPRSFGTFPRFLRLVREHQLM
                     PLEDAVYKLTGLSADILGLRDRGTLAEGRIADITVFDAERVRDTSVFTDSVRKPEGIC
                     HVLVGGEFALRQGRSAGGRKGAVIRRESGGPV"
     gene            3339..>4126
                     /locus_tag="ACZPAQ_03800"
     CDS             3339..>4126
                     /locus_tag="ACZPAQ_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR0903139.1"
                     /translation="MELGSWSIIQAPSLLALIPLVVMIVLIFRGKSNVSAIMVGVLVG
                     ALLLGQDLGAMAKAFATSLGSSTALIGLIIMMGAGLGVLMSEAGVTHTLVYWIVKRIG
                     VNTQTKGKIALVVVSIIVCGLLGTLGGGNAVIAPIMLPIMASLGVTPTVVATLFKVAG
                     EIGLILGPLTGVTLITMEVTGLSYGELMIQAAIPFAVFWLAGAWVGANRAQKRTFGKE
                     GYALGEDVQHLDQVVITPRQKRTTVAFLISFVLLVGYGIATKQGT"
BASE COUNT          703 a         1336 c         1438 g          649 t
ORIGIN      
        1 cattcagggc ctctacccgg aagaggagac gccggccttt gagggccggg ttatcgacgg
       61 cgggggccgg tatctggccc ccggcttcat cgacatccac actcactctg acgcctgccc
      121 cctggtggac tatgagccgg agagcaagct acaccagggc atcaccaccg agatcaccgg
      181 caactgcggc atctccatcc tgcccagcac gccggagagc cgggacgaga tcggccgcta
      241 cttcttcagc cagctggagc tgcccgccgg gagcctgccc cagctgggag tgtccggcct
      301 ggaggagtac gcccggatgg tggcggacca cggctgcgcc gccaactacg gccagctggt
      361 gggccacggc accctgcggg gggcggtgat gggctttgtg gaccgggacc ctgaaccgga
      421 ggagatggag cagctgaagg acctgctgga gcgggagctg aaggcgggcg ccttcggtat
      481 gtccctgggg ctgatctatc cccccagcgc cttctgcaag agcgaggagc tggtggagct
      541 ggccaaagtg ctgaagaagt acgacgccct gctcaccgtc cacatgcgca acgaggggcc
      601 ccgcatcttc caggcagtgg atgagatgat cgacatcacc cgccgctccg gggtgcacct
      661 gcagatctct cacctgaagc tgatgggcaa gccccagtgg ggccgctccc aggagctact
      721 ggacaagatc gaggcggccc ggcaggaggg gatgaccatc acctgtgacc agtaccccta
      781 cactgccacc tccacctcca tgaccgccct gctgcccaag tgggcccacg acggcggcgt
      841 gtccgccctg gtggcccggg tcaaggcccc cgaccagcgg ctgaaggacg agacggcggc
      901 ggagatggag gaccggggcg gcccggccca tgtgatggtc tccggcaccc acggctacca
      961 cccggagtgg gagggcagga ccgtggcgga gctggcggag gagctggggc tgtccccggt
     1021 ggacacagtg atgcatgtgc tgtccgcctg cgagggctcg gtggcctgca tctacttctg
     1081 catcaacgag gacgacatgc tgaacatcat gagggacatg cgcatcgcac tgggcagcga
     1141 cggatacggc ttctcctatg accggtccat cacccggacc aacccccatc cccgcagctt
     1201 cggcaccttc ccccgcttcc tggagctggc ccgggaccac aggctgatgc ccctggagga
     1261 cgcggtgtac aagatcaccg gcctgccggc ggacatcctg ggcctgaagg accggggcac
     1321 cctggaggag ggccggatcg ccgacatcac ggtctttgac ccggagcagg tgcgggacct
     1381 gtcggagtac accgactcgg tgaaaaagcc ggcgggcatc cgccacgtgc tggtgggggg
     1441 caccttcgcc ctgaaggacg gcgcggctac cggctccaga acgggcacgg tgctgaagaa
     1501 ggtctgagac aatctggcct gccaatatag agggaccgcc gcggcggtca tccctcccct
     1561 ttccggggag agatggccgg gggagctgag ggcggctccc ccggcgggcc tctggagaaa
     1621 aaaggaggga aggagccttg tatgatctga tcctgcgggg cggcacggtg gtggacggaa
     1681 cgcgttcccg gccctatgcc gccgacgtat gcatccggga cgggcgcatc gcccgcatcg
     1741 ccccggatgc gggggaggag gcacgggagg tggtggacgt gtccgggctg acggtggccc
     1801 cgggcttcat cgacatccac agccactccg atgcgtgccc cctgctcagc ttccagccgg
     1861 agagcaagct cttccagggc atcaccaccg agatcaccgg caactgcggc acctccatcc
     1921 tgcccagcct gccggagaac agccgggaga tcgtccaata cttttttgat gacaccagca
     1981 tgttcagtca ggtggtcctc aacgagaagg accggagcct ggacggcctg tacggggtgg
     2041 aggagtatgc ccgggcggtg gcggcccacg gctgcaccgc caactacggc cagctggtgg
     2101 gccacggcac cctgcggggg gcggtgatgg gctttgtgga ccgggaccct gaaccggagg
     2161 agatggagcg gctgaaggac ctgctggagc gggagctgag ggcgggcgcc ttcggcatgt
     2221 ccctggggct gatctatccc cccagcgcct tctgcaagcg ggaggagctg gtggagctgg
     2281 ccaaggtgct gaagaagtac gacgccctgc tcaccgtcca tatgcgcaac gaggggcccc
     2341 gcatcttcca ggcagtggac gagatgctgg acatcacccg ccgctctgga gtgcacctgc
     2401 agatctccca cctgaagctg atgggcaggc cccagtgggg gcgctcccgg gagctgctgg
     2461 acaagctggc ggccgcccgg cgggaggggc tgaacgtcac ctgcgaccag tacccctata
     2521 ccgccacctc cacctctatg accgccctgg tgcccaagtg ggcccacgac ggaggccgcg
     2581 ccgccctgct ggagcggctg cgggacccct cccggcagct gaaggcggag atcaccgccg
     2641 agatggagga ccgcggcgga gcggaccggg tgatgatcgc cggcactcac ggctgccgcc
     2701 cggagtggga gggcaggacc gtggcggagc tggcggagga gctgaagctg tccccggcgg
     2761 acacggtggt ccacgccctg gactgctgcg gcggccgggt ggcctgcatc tacttctcca
     2821 tcgatgaggg cgacatgctc aacatcatga aggacatgga cattgcggtg ggcagcgacg
     2881 gatacggcct gtcctttgac cgctccatta ccatcaccga cccccacccc cgcagcttcg
     2941 gcaccttccc ccgcttcctg cggctggtgc gggagcacca gctgatgccg ctggaggacg
     3001 cggtgtacaa gctgaccggc ctgtcggcgg acatcctggg cctgagggac cggggcaccc
     3061 tggcggaggg ccggatcgcc gatatcacag tctttgacgc ggagcgggtc cgggacacct
     3121 cggtgttcac cgattccgtg cggaagccgg agggcatctg ccatgtgctg gtggggggcg
     3181 aattcgccct gcgccagggc aggtctgccg gagggagaaa gggggcggtc atccggcgcg
     3241 agagcggagg acctgtctga ggacagggag aaaacaatat gtgtgagccg tcccggcctg
     3301 cctgtctgga ccggggcgga cagaagggag aaacagatat ggaattaggt agttggtcta
     3361 tcattcaggc gccctctctg ctggctctga tccccctggt ggtcatgatc gtgctgatct
     3421 tccgggggaa gagcaacgtg tccgccatca tggtgggcgt gctggtgggc gctctgctgc
     3481 tggggcagga cctgggggcc atggccaagg ctttcgccac ctcgctgggt tcctccacgg
     3541 ccctgatcgg cctcatcatc atgatgggcg ccggcctggg cgtgctgatg agcgaggcgg
     3601 gggtcaccca caccctggtc tactggatcg tcaagcgcat cggcgtgaac acccagacca
     3661 aaggcaagat cgccctggtg gtggtgtcca tcatcgtctg cggcctgctg ggcaccctgg
     3721 gcggcggcaa cgccgtcatc gcccccatca tgctgcccat catggcctcc ctgggggtca
     3781 cccccaccgt ggtggccacc ctgttcaagg tggcgggtga gatcggcctg atcctgggcc
     3841 ccctcaccgg cgtgaccctc atcaccatgg aggtcaccgg cctgtcctac ggcgagctga
     3901 tgatccaggc ggccatcccc ttcgccgtgt tctggctggc tggcgcctgg gtgggcgcga
     3961 accgcgccca gaagcgcacc ttcggcaagg agggctatgc cctgggggag gacgtgcagc
     4021 acctggatca ggtggtcatc accccccgtc agaagcggac cacggtcgct ttcctgatct
     4081 cctttgtcct tctggtgggc tacggcatcg ccaccaagca gggcac
//
LOCUS       DBMDSJ010000110         4110 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5700_length_4110_cov_1.458718, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000110 DBMDSJ010000000
VERSION     DBMDSJ010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5700_length_4110_cov_1.458718"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="ACZPAQ_03805"
     CDS             complement(<1..125)
                     /locus_tag="ACZPAQ_03805"
                     /inference="COORDINATES: protein motif:HMM:NF048942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrrC family ATP-dependent DNA helicase"
                     /protein_id="HIR0903140.1"
                     /translation="MEGTVASLIFQNEENGYTILRLERGGEELTLVGEMPGVSPG"
     gene            complement(176..499)
                     /locus_tag="ACZPAQ_03810"
     CDS             complement(176..499)
                     /locus_tag="ACZPAQ_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903141.1"
                     /translation="MEYVWEIVLTVLCLMGLGLLVWCLMGRLVCPVPGQEVLAVLEGQ
                     GDGGGLEQSVRALMWLRSLGLLRCPVVIVDLGLDREGLAVAQRLALRWPVVDLYRPED
                     GAEAH"
     gene            complement(546..1034)
                     /locus_tag="ACZPAQ_03815"
     CDS             complement(546..1034)
                     /locus_tag="ACZPAQ_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR0903142.1"
                     /translation="MDLFQWLTDTTEGKCLFTTLVSMIPIIELRGGIPFGVALGLPYW
                     LAFPAAVIGNVIPAPFIIVYIRRIFAWMRRHLPRLNSMVDKLEEKAHLKGQKVRRYQY
                     LGLWIFVAIPLPGTGAWTGSLVAAFLNMRLKQALPAVVLGVMTAGCIMTALTHVGINL
                     FA"
     gene            complement(1112..1327)
                     /locus_tag="ACZPAQ_03820"
     CDS             complement(1112..1327)
                     /locus_tag="ACZPAQ_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903143.1"
                     /translation="MTEVILPGLAGRLKPLRNEKKMTQKEMAALLDCTEGHYQKIEYG
                     KINLSALQLMELADFFGVTTDYLLGRD"
     gene            1411..1674
                     /locus_tag="ACZPAQ_03825"
     CDS             1411..1674
                     /locus_tag="ACZPAQ_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903144.1"
                     /translation="MEDFLSWFYEHYIKTTLSSQPPDFGTACRADLLRSVLDPFLQQQ
                     LEELNRFYALRSFRLGLRTGLALARDLEDGPLTPPPPSEGGLP"
     gene            complement(1637..2182)
                     /locus_tag="ACZPAQ_03830"
     CDS             complement(1637..2182)
                     /locus_tag="ACZPAQ_03830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903145.1"
                     /translation="MEHIRIIAPGDPGFDWLYDLYMQSFPVHEQRLRERQEGVLGHPE
                     YHCDLLCQGETRVGLLMWWAGPGFRYVEHFAIAPQLRGQSWGSRALREFLAEGDPVVL
                     EIDPPEDPVSRRRKGFYEGLGLVSNPWPHIHPPYRLEFSGHALEVMTFPGGWDEGQYR
                     RFARYLDETVMADRPQKVAGA"
     gene            complement(2387..>4110)
                     /gene="uvrA"
                     /locus_tag="ACZPAQ_03835"
     CDS             complement(2387..>4110)
                     /gene="uvrA"
                     /locus_tag="ACZPAQ_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR0903146.1"
                     /translation="GTLYQAFEGIVNNLGRRYRETQSDSVKRELEECMSECPCPTCEG
                     RRLRKESLAVTVGGIDIDAFTHKSVDDALEFVDQLTLTETQMRIAQRILKEIRERLGF
                     LRSVGLQYLTLSRSAASLSGGESQRIRLATQIGSSLMGVLYILDEPSIGLHQRDNDML
                     LETMKHLRDLGNTLLVVEHDEDTMRAADYIVDVGPGAGVHGGQIVACGTAEEIMNTPG
                     SLTGDYLSGRRKIPVPEQRRKGNGKYLKVLGAAENNLRHIDVSFPLGTFTAVTGVSGS
                     GKSSLVNEILYNRLACDLNRAKTRPGRHDGLEGEEFLDKVINIDQSPIGRTPRSNPAT
                     YTGLFNDIRDLFASTQDAKSRGYGPGRFSFNVRGGRCEACQGDGLLKIEMHFLPDIYV
                     PCEVCKGKRYNRETLEVRYKGKNIYEVLEMTVEEALPFFENLPRIYGRLKTLMDVGLS
                     YIKLGQPSTELSGGEAQRVKLATELSKRSTGQTIYILDEPTTGLHSYDVHRLIEVLQR
                     LVEAGNTVVVIEHNLDVIKTADHVIDLGPEGGDGGGTIVCTGTPEEVAACERSYTGRY
                     LKKMLER"
BASE COUNT          731 a         1390 c         1208 g          781 t
ORIGIN      
        1 tccccggggg agacgccggg catctccccc accagggtga gctcctcgcc cccccgctcc
       61 agccggagga tggtgtagcc gttctcctca ttctgaaaga tcaaagaggc cacggtgccc
      121 tccaccagtt ccagctctct gccgtcctcc aattttgggc acctcctctc ttgtatcaat
      181 gtgcctccgc cccatcctcc gggcggtaca ggtccaccac cggccagcgc agggcgagcc
      241 gctgggccac cgccaggccc tcccggtcca gacccaggtc tacgatgacc accgggcagc
      301 gcagcaggcc caggctcctc agccacatca gggcgcggac gctctgctcc agcccccctc
      361 cgtccccctg cccctccagg acggccagga cctcctgccc ggggaccggg cacaccagcc
      421 gccccatcag gcaccagacc agcaggccca gccccatcag gcacaggacg gtcagcacga
      481 tctcccacac atattccata caggctcacc tccggcccat cctatgccgg aagcgccgcc
      541 ggggttcagg cgaacagatt gatacccacg tgggtcagag cagtcatgat gcaccccgcc
      601 gtcatcaccc ccagcaccac agcgggcagg gcctgtttca gccgcatgtt cagaaaggcg
      661 gccaccagag agccggtcca tgcgccggtt ccgggcaggg gaattgccac aaagatccaa
      721 aggcccagat actggtagcg ccgcaccttc tgtcccttca ggtgggcctt ctcctccagc
      781 ttgtccacca tgctgttcag gcggggcaga tgtctgcgca tccaggcgaa gatgcggcgg
      841 atgtacacga taatgaaggg ggcggggatc acgttcccga tcaccgcggc gggaaaggcc
      901 agccagtagg gcaggcccag ggccacgccg aaggggatgc cccctctcag ctcgatgatg
      961 gggatcatag acaccagggt ggtgaacagg catttcccct ctgtagtgtc ggtcagccac
     1021 tgaaaaagat ccattctggt ctcctgtcaa atccgtattt tgccgcgccg cggcaagctg
     1081 ataaccaatt tacacgatac cggtccatcc gtcaatcccg gcccagcaaa taatcggtgg
     1141 tgacgccgaa aaagtcggcc agctccatca gctggagggc agagagattg atctttccat
     1201 actcgatctt ttgataatgt ccctctgtgc agtccagcag agcggccatc tctttctggg
     1261 tcatcttttt ttcgtttcgc agcggcttca gccggcccgc cagccccgga aggatcactt
     1321 ctgtcatttt taatctcctc ttaacacatt attaaatgat gtattataat aacatcgcaa
     1381 tatgatgtgt ttgaaggaca ctgatttgat atggaagact ttctatcttg gttctatgag
     1441 cactatatca aaactaccct ttcctctcag cccccagatt tcgggactgc ctgccgggcc
     1501 gacctgctcc gaagcgtgct ggaccccttt ctccagcaac agctggaaga gctcaaccgc
     1561 ttttacgccc tccgcagctt ccgtctggga ctacggacag gactggcgct ggcccgggac
     1621 ctggaggatg gaccacttac gcccccgcca ccttctgagg gcggtctgcc atgaccgtct
     1681 cgtccagata tcgggcaaag cgccggtact gtccctcgtc ccagccgccg gggaaggtca
     1741 tcacctccag ggcgtggccg gagaactcca ggcggtaagg gggatggatg tggggccatg
     1801 ggttggagac aaggcccagc ccctcgtaaa agcccttcct gcgccgggat accgggtcct
     1861 ccggaggatc gatctccagc accaccgggt ctccctccgc caggaactca cgcagtgccc
     1921 ggctgcccca gctctggccc cgcagctgcg gggcgatggc gaaatgctcc acatagcgga
     1981 agccgggccc ggcccaccac atcaacagcc ccacccgtgt ctccccctga cagagcaggt
     2041 cgcagtggta ctccggatgg cccagcaccc cctcctgccg ttcccgaagg cgctgctcgt
     2101 ggacgggaaa gctctgcata tagaggtcat agagccagtc gaagccagga tcgcccgggg
     2161 cgatgattcg gatgtgttcc ataggatcct cctcctgatt ttctgcggcg ctccggcaaa
     2221 ggccgccgga gcgctccgcg cgttatatat tatgatgggt atataggggg ccgtccggcc
     2281 cgatgggggc atccggctct gcgggggcgc atttatttcc gattctcacg cagggacccg
     2341 cggcctgtcc gttctgttga tctggcccag gcagaaggac gcgccctcac cgctccagca
     2401 tctttttcag ataccgcccg gtgtagctcc gctcacaggc ggccacttcc tccggcgtgc
     2461 ccgtgcagac gatggtgccg ccgccgtcgc ccccctcggg gcccaggtcg atgacatggt
     2521 cggcggtctt gatgacatcc aggttgtgct cgatgaccac cacggtgttg cccgcctcca
     2581 ccagacgctg gagcacctcg atgagccggt ggacatcata gctgtgcagg ccggtggtgg
     2641 gctcatccag gatatagatg gtctggccgg tggagcgctt ggacagctcg gtggccagct
     2701 tgacccgctg ggcctcgccg ccggacagct cggtggaggg ctgccccagc ttgatatagc
     2761 tcagccccac atccatcagg gtcttgagcc gcccgtagat ccggggcagg ttctcgaaga
     2821 aggggagggc ctcctccact gtcatctcca gaacctcata gatatttttc cccttatacc
     2881 gcacctccag ggtctcacgg ttgtaccgct tgcccttgca cacctcgcag gggacgtaga
     2941 tgtccggcag gaagtgcatc tcgatcttca gcaggccgtc cccctgacat gcctcgcacc
     3001 ggccgccccg cacattgaag gagaagcggc ccgggccgta gccccggctt ttagcgtcct
     3061 gggtggaggc gaacaggtcc cggatgtcgt tgaacagccc ggtgtaggtg gccggattgg
     3121 accggggcgt gcgcccgatg ggggactggt cgatgttgat gaccttgtcc aggaactcct
     3181 ccccctccag gccgtcgtgt ctgcccggcc gagtcttggc ccggttcagg tcgcaggcca
     3241 gccggttata gaggatctcg ttgaccaggg aggactttcc gctgcccgac acgccggtca
     3301 ccgcagtgaa ggtccccaga gggaaggaca cgtcgatgtg gcgcagattg ttctccgccg
     3361 cccccagcac cttcaggtac tttccgttcc ccttccggcg ctgctccggc accgggatct
     3421 tccgccgccc ggagaggtag tcgccggtga gggagccggg ggtgttcatg atctcctccg
     3481 ctgtgccgca ggccacgatc tgtccgccgt ggacgccggc cccggggccc acgtccacga
     3541 tatagtcggc ggcccgcatg gtgtcctcat cgtgctccac caccagcagg gtgttgccca
     3601 ggtcccgcag gtgcttcata gtctccagca gcatgtcatt gtcccgctgg tgcaggccga
     3661 tggagggctc gtccagaata tacagcactc ccatcagaga ggagccgatt tgggtggcca
     3721 gccggatgcg ctggctctcg ccgccggaca ggctggcggc gctgcgggac agggtcaggt
     3781 actggagccc caccgaccgc aggaagccca gccgctcccg gatctccttc aggatccgct
     3841 gggcgatgcg catctgggtc tcagtcaggg tcagctgatc cacgaactcc agggcgtcgt
     3901 ccactgattt gtgggtgaag gcgtcgatat cgatcccccc cacggtgacc gccagagact
     3961 ccttgcgcag gcggcggccc tcacaggtgg gacacgggca ctcgctcatg cactcctcca
     4021 gctcgcgctt tacagagtcg gactgagttt cccggtagcg gcgccccagg ttgttgacaa
     4081 taccctcaaa cgcctgatac agggtcccct
//
LOCUS       DBMDSJ010000111         4049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5792_length_4049_cov_1.585360, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000111 DBMDSJ010000000
VERSION     DBMDSJ010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5792_length_4049_cov_1.585360"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="ACZPAQ_03840"
     CDS             <1..347
                     /locus_tag="ACZPAQ_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903147.1"
                     /translation="VEMGEAGGENMEMDSIQKVAGAEQESQERREAAAAESRRTVAEA
                     RKAAEELLEEARRQAGAETRQMLARAEADAAEETKTILAEAEQRCQALKQNARTRMDQ
                     AAKRIVEGVVKG"
     gene            347..2287
                     /locus_tag="ACZPAQ_03845"
     CDS             347..2287
                     /locus_tag="ACZPAQ_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR0903148.1"
                     /translation="MAIVKMKHLRLAAMQSDREALLRLLQGMGCLEIEEPSVDWSDPE
                     WAGLKSPDSGALSAAREEKNAAQHALEVLKRYAKVKGGLLSPRPVVSPQQFFEEQAYR
                     SALETARAINDGERELAALKAERDKRAAQRASLAPWIPLELPLDTASTREVEVQFGTV
                     SAETPFPQLAGEVQAVSELAQLTLASADRENQYLLLICHRSALDEVLEKLKEHGWSRA
                     VFRDLTGTAADNDRRLEQEMEELSQRRAEQERRIAAQAGQREALKRAVDRATVEIDRE
                     EGKSRLVETQVAFFLEGWVPEERWDQLRQALSDYPCAWEVRDPEPEEYPQVPVQLKNN
                     WLTRPLNMVTEMYSLPAYGSVDPNPLMAPFFILFYGIMMADMGYGLLMMLASVVVLRR
                     MRPKGTMSHFFGLLGLCGVSTFLMGAVTGGFFGDMIPQVLKILNPESTFVWFWPPLFT
                     PLDDTLMILVGAMALGLIQIVTGMAISFIKKIREGQILDAVWEELTWWIVFAGLALAV
                     LGVTNVVIILGGVMVLVGSGWNAKGFGKVTAVFGSLYNHVTGYFGDILSYSRLMALML
                     AGSVIAQVFNTLGAIPGNLVIFFIISLAGNALNFALNLLGCYVHDLRLQCLEYFGKFY
                     QDGGKPFRPMAVNTRYVDIKEQ"
     gene            2306..2830
                     /locus_tag="ACZPAQ_03850"
     CDS             2306..2830
                     /locus_tag="ACZPAQ_03850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HIR0903149.1"
                     /translation="MDMSQVVEYGQSMTFLGSIGGLALALLGAGLAAVLSGIGSAKGT
                     GLAGEAGTGLLCEDPSKFGKVMILQVIPGTQGLYGLVVWFFAIFRMGLLSGTLPQLTI
                     AQGMQYFVACLPMALGGLFSAIAQGRVAAGSINILAKKPDDWSKGMVLCITVEFYAIL
                     SLLASMLMIINISA"
     gene            2850..3539
                     /locus_tag="ACZPAQ_03855"
     CDS             2850..3539
                     /locus_tag="ACZPAQ_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR0903150.1"
                     /translation="MDGLEKITARISADADAEIHAIRDKAQAQAEELLAQARAQAEKE
                     KGELLARGRRSAEERKARLISAAQMEGRKMSLAAKQEVLDEAFRQALEEMCSLPEEQY
                     IQLLARLAVQASSTGREQLIFSPKDRTRIGKAVVMAANDALVKQVAPELPDAITDSKV
                     GAFLGKVVNSAAAQITGTGLLTLSEETRPIRGGFIMVDGPVEVNCAFEAMVRAQQEQL
                     EKPVAEILFQK"
     gene            3662..>4049
                     /locus_tag="ACZPAQ_03860"
     CDS             3662..>4049
                     /locus_tag="ACZPAQ_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR0903151.1"
                     /translation="MSQKRDTDYLAVSTRIHAMENRMLTRERMERMIDAKDSGEAAKV
                     LSECGYGELTQLTSAALEELLADARRNTFRDMADSVPDRRLVEVFQLKYDYHNAKVLI
                     KAQAMGLDEARLLSQGGRYDPQRLRED"
BASE COUNT          750 a         1160 c         1453 g          686 t
ORIGIN      
        1 gcgttgagat gggggaagcg ggtggtgaga atatggaaat ggactccatt caaaaggtag
       61 ccggcgcaga gcaggagagc caggagcgcc gggaggcggc tgcggcggag agccgacgga
      121 ccgtagctga ggcccggaag gcagctgaag agctgctgga ggaggcccgc cgccaggcgg
      181 gggctgagac ccggcagatg ctggcccggg cggaggcgga cgccgcggag gagacgaaga
      241 ccatcctggc ggaggcggag cagcgctgcc aggcgctgaa gcagaacgcc cggacccgta
      301 tggatcaggc ggcgaagcgc atcgtggaag gggtagtgaa gggctgatgg cgatcgtcaa
      361 aatgaagcat ctgcgcctgg cggccatgca gtcggaccgg gaggccctgc tgcggctgct
      421 tcaggggatg ggctgcttgg agatcgaaga gccgtcggtg gactggagcg atccggagtg
      481 ggccgggctg aagagcccgg acagcggggc gctctccgcc gcccgggagg agaaaaacgc
      541 agcccagcac gccctggagg tgctcaagcg ctacgcaaag gtgaaggggg gcctcctgag
      601 ccccaggccg gtggtctccc cccagcagtt ttttgaggag caggcgtacc gaagcgccct
      661 ggagaccgcc agagccatca atgatgggga gcgggagctg gcggcgctga aggcggagcg
      721 ggacaagcgg gctgcccagc gggcctccct ggcaccgtgg atccccctgg agctccccct
      781 tgacacggcc tccacccggg aggtggaggt gcagttcggc acggtatcgg cggagacccc
      841 cttcccccag ctggcggggg aggtgcaggc agtgagtgag ctggcccagt tgacgctggc
      901 cagcgcagac cgggaaaacc agtatctgct gctgatctgc caccgttccg ccttggacga
      961 ggtgctggag aagctgaagg agcacggctg gtcccgggca gtgttccggg atctgacagg
     1021 cacggcggca gacaacgacc gccggctgga gcaggagatg gaggagctgt ctcagcgcag
     1081 ggcggagcag gagcggcgga tcgccgccca ggccggccag cgggaggcgc tgaagcgggc
     1141 ggtggaccga gccacggtgg agatcgaccg tgaggagggc aagagccgcc tggtggagac
     1201 ccaggtggcc ttcttcctgg agggctgggt gccggaggag cgctgggacc agctgcggca
     1261 ggccctgtcc gactacccct gtgcctggga ggtccgggac ccagagccgg aggaatatcc
     1321 ccaggtgccg gtgcagctga aaaacaactg gctcacccgg ccgctgaaca tggtcacgga
     1381 gatgtactcc ctgcctgcct acggaagcgt ggacccaaac cccctgatgg cccccttctt
     1441 tatcctgttt tacggcatca tgatggcgga catgggctat ggcctgctga tgatgctggc
     1501 ctcggtggtg gtcctgcgcc ggatgagacc caagggcacc atgagccact tcttcggtct
     1561 gctgggactg tgcggcgtca gtaccttttt gatgggagcg gtcacgggag gctttttcgg
     1621 tgacatgatc cctcaggtgc tgaagatcct gaatccggag agcaccttcg tctggttctg
     1681 gccgcccctg ttcacccccc tggatgacac cctgatgatc ctggtggggg ccatggccct
     1741 gggcttgatt caaatcgtca cgggcatggc tatcagcttc atcaaaaaga tcagggaggg
     1801 gcagatcctg gatgcggtat gggaggagct gacctggtgg atcgtctttg ccggtctggc
     1861 cctggcagtg ctgggggtga ccaacgtggt catcatcctg ggcggcgtca tggtgctggt
     1921 gggctcgggc tggaacgcca agggcttcgg taaggtgacc gccgtgttcg gctccctgta
     1981 taaccatgtg accgggtact ttggggacat cctctcctat tctcgtctca tggccctgat
     2041 gctggcaggc agcgtcatcg cccaggtgtt caatactctg ggcgcgatcc cgggcaacct
     2101 ggtgatcttt ttcatcatct ccttggcagg caatgccttg aactttgcct tgaatctgct
     2161 gggctgctat gtgcatgacc tgcgtctgca gtgcctggag tattttggaa agttctatca
     2221 ggatggcgga aagcctttcc ggcctatggc ggtgaacacc cggtatgtgg atatcaaaga
     2281 acaatgattt gaggaggata caaaaatgga tatgtcacaa gtggtagagt atggacagag
     2341 catgactttc ctgggcagca tcggcgggct ggccctggcc ctgctggggg ctggactggc
     2401 ggctgtactc agcggcatcg gcagcgcaaa gggcaccggc ctggccggcg aggcgggcac
     2461 cggcctgctg tgtgaggatc cctccaagtt cggcaaggtg atgatccttc aggtcatccc
     2521 cggcacccag ggcctgtacg gcctggtagt ctggttcttc gccatcttcc gtatgggcct
     2581 gctgtctggg accctgcctc agctgaccat cgcccagggg atgcagtatt tcgtggcctg
     2641 cctgcctatg gcgctgggcg gcctgttctc cgccatcgcc cagggccgtg tggccgccgg
     2701 ctccatcaac attctggcca aaaagccgga tgactggtcc aagggcatgg tgctgtgcat
     2761 cactgtggag ttctacgcca tcctctccct gctggcctcc atgctgatga tcatcaacat
     2821 cagcgcctga gcgccggaaa ggaacagcta tggacggact tgaaaagatc acggccagga
     2881 tcagcgccga cgcggacgcg gagatccatg ccattcgaga caaggcccag gcccaggcgg
     2941 aagaactgct ggcccaggcc agggcccagg cggagaagga gaagggcgag ctgctcgccc
     3001 ggggccgccg gtccgcggag gagcggaagg cccgcctgat ctctgccgct cagatggagg
     3061 ggcggaagat gtccctggct gccaagcagg aggtgctgga cgaggcgttc cgccaggctc
     3121 tggaggagat gtgctcgctg ccggaggagc agtacatcca gctgctggcc cgcctggcgg
     3181 tgcaggcgtc ctccaccggc cgggagcagc tgatcttctc ccccaaggac cggacgcgta
     3241 tcggcaaggc ggtggtgatg gcggccaacg acgctctggt gaagcaggtg gctccggagc
     3301 tgcccgacgc catcaccgac tccaaggtgg gggccttcct gggcaaggtg gtcaacagcg
     3361 ctgcggcgca gatcaccggc accgggcttt tgaccctgtc cgaggagacg aggcccatcc
     3421 ggggcggctt catcatggtg gacggcccgg tggaggtcaa ctgcgccttt gaggccatgg
     3481 tgcgtgccca gcaggagcag ctggaaaagc cggtggcgga gatcctgttt caaaagtgaa
     3541 gagtgaggag cggagcgggg gaacggaggg cccgggtgcc cattgccaga ttccgcagga
     3601 agcaaagact gcccgcagag atacgcgcgg cgctctgctc tgacccgaaa ggggtggtat
     3661 tttgtcccag aaacgagata cggattattt agccgtctcc acccgcatcc acgcgatgga
     3721 aaaccgcatg ctgacccggg aacggatgga gcggatgatc gacgccaagg acagcggcga
     3781 agcagccaag gtgctcagcg agtgcgggta cggcgagctg acccagctga cctcggcggc
     3841 gctggaagag ctgctggccg atgcccggcg gaacaccttc cgggacatgg cggactcggt
     3901 gcctgaccgg cggctggtgg aggtctttca gctgaaatac gactaccaca acgccaaggt
     3961 gctgatcaag gcccaggcca tggggctgga cgaggcgcgt ctgctctccc agggcgggcg
     4021 gtacgatccc cagcggctgc gggaggatt
//
LOCUS       DBMDSJ010000112         4039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5806_length_4039_cov_1.384205, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000112 DBMDSJ010000000
VERSION     DBMDSJ010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5806_length_4039_cov_1.384205"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="ACZPAQ_03865"
     CDS             complement(<1..782)
                     /locus_tag="ACZPAQ_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903152.1"
                     /translation="MTRPCPYTPLSTPLSGSARQAEARIRNLFQGPKRRPPVWLMVLA
                     LLLILSCGGLVTCQDQKVPPEKAEISALSASATSQPLSSEDLLLVDALYGAAAPQMGT
                     AEDGESIPQLLCAHSQDGTTLAAALFQKDSASWLVIGAVEQATSTLRGPAYVVSGPEG
                     AAHIFPFREYGSGQLRLLYTFNTLDQGSRSGSAGVVCLEDSRLTWAWPAEGDILEEGS
                     RAAQEYEEFWQTYLPLLSPSGVELFSRETASSPYGGSPAHWT"
     gene            complement(779..1690)
                     /locus_tag="ACZPAQ_03870"
     CDS             complement(779..1690)
                     /locus_tag="ACZPAQ_03870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR0903153.1"
                     /translation="MNRFLLDLAALSLGGGAVVLLLLLAARLTRGRYAARWRCTAWLL
                     LSLRLAVPFPLLPHLGPVEAPVQLTVPSNPVVFTYSPPVSQPPAASSRPAQSQAAPSR
                     SEPVQNAVQPEAPAESGGFSLSLAQLLLILWLLGAAGVLLWNVGCHLRFCRWLRRWGR
                     EAEETPLLARYRALGDQMGLRRLPRLVLCPSLPAPMLAGLLRPVILLPEPLPQSAPLE
                     CALRHELTHFRRRDIPLKALVLWVKALHWFNPLMWLMARAVEQDTELACDDSTLHALP
                     GDQRSAYGRSILQAVSAVQNRKEEDQS"
     gene            complement(1687..2058)
                     /locus_tag="ACZPAQ_03875"
     CDS             complement(1687..2058)
                     /locus_tag="ACZPAQ_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR0903154.1"
                     /translation="MKRLPDTELEVMKALWDSGPDTPRAALERALAPHGWAANTINTY
                     LTRLADKGFLSARRLGRSNRYTPLVSREDYLSFDSRSVLSRLYGSSPRNFVAALARDG
                     LAPGEVDELRALLDELERGAK"
     gene            complement(2248..2762)
                     /locus_tag="ACZPAQ_03880"
                     /pseudo
     CDS             complement(2248..2762)
                     /locus_tag="ACZPAQ_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493271.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
     assembly_gap    2763..2772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2773..2919)
                     /locus_tag="ACZPAQ_03885"
     CDS             complement(<2773..2919)
                     /locus_tag="ACZPAQ_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HIR0903155.1"
                     /translation="MNVTFEMVRSSPEIRTYITQADASLTALGFTEHSFAHVTKCAEV
                     AARIL"
     gene            complement(2916..3251)
                     /locus_tag="ACZPAQ_03890"
     CDS             complement(2916..3251)
                     /locus_tag="ACZPAQ_03890"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR0903156.1"
                     /translation="MTPGRPDFFTAVYAVVARIPAGQVATYGLVAFLAGRPRASRIVG
                     CALARAPEGLPCHRVLYRDGGLRPADVFGGTGIQRLLLEGEGVPFLPDGRVDLSRCLW
                     DGGEERSTL"
     gene            3709..>4039
                     /locus_tag="ACZPAQ_03895"
     CDS             3709..>4039
                     /locus_tag="ACZPAQ_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR0903157.1"
                     /translation="MKLSSFLHFAAFGVRTILLREKKPILGTVIVTDRCNLKCRHCSV
                     HDLTSVDHPYEQIRREMELLYHMGVRILFFCGGETFLWHSDGHSLRDLVWEAKQMGFL
                     IVNVVTNG"
BASE COUNT          770 a         1168 c         1449 g          642 t
ORIGIN      
        1 taggtccaat gggcgggaga gcccccgtag gggctggagg ccgtttcccg ggagaacagc
       61 tccacaccgg agggggacag caagggcaga taggtctgcc agaattcctc atattcctga
      121 gcggcccggg agccctcctc caggatgtcc ccctccgccg gccacgccca ggtgagccgg
      181 ctgtcctcca ggcacactac cccggcggag ccggagcggc tgccctgatc cagggtgttg
      241 aaggtgtaga gcaggcggag ctggccgctg ccgtactccc ggaacgggaa gatatgggcg
      301 gccccctccg gtccggagac cacataggcc gggccccgta gggtgctggt cgcctgttcc
      361 actgccccga tgaccagcca ggaggcggag tctttctgga agagagcggc ggccagggtg
      421 gtcccgtcct ggctgtgggc acagagaagc tgggggatgg attctccgtc ctctgccgta
      481 cccatctggg gagcggcggc cccgtacagg gcgtctacca ggaggaggtc ctcactggag
      541 agaggctggg aggtggcgga ggcagagagg gcgctgatct ccgccttttc cggcggaacc
      601 ttttggtcct ggcaggtcac cagcccgccg caggacagga tgaggagcag ggccaggacc
      661 atcagccaga ctggcggtcg ccgcttgggg ccctggaaca ggttgcggat gcgcgcctcc
      721 gcctgccggg cggagccgga gaggggggta gagaggggcg tgtaaggaca tgggcgggtc
      781 aagactggtc ctcctccttt ctgttttgga cggcgctgac ggcctgaagg atggaacggc
      841 cgtaggcaga acgctggtcc ccgggcagcg cgtgcagggt gctgtcgtca caggccagct
      901 ctgtgtcctg ctccaccgcc cgggccatga gccacatcag cggattgaac cagtgcaggg
      961 cctttaccca gagcaccagg gccttcaggg ggatgtctcg gcggcggaag tgggtcagct
     1021 cgtggcgcag ggcgcactcc agaggggcgc tctgggggag gggctccggt agaaggatca
     1081 cagggcggag cagaccggcc agcatggggg cgggcagaga ggggcagagg accagcctgg
     1141 gcagacggcg caggcccatc tggtccccca gcgcccggta ccgcgccagc aggggagtct
     1201 cctcagcctc ccggccccag cggcggagcc agcggcagaa cctgaggtga cagcccacat
     1261 tccacaggag gacccccgcc gcgcccagca gccacaggat caggaggagc tgggcgaggg
     1321 agagggaaaa tcctcctgat tccgccgggg cctccggctg gacggcattc tggaccggct
     1381 cagaccggga gggcgcggcc tgggactggg ctgggcggct ggaggccgcc gggggctggg
     1441 agacaggcgg cgaataggtg aagaccaccg ggttggaggg gacggtcagc tggactggtg
     1501 cctcgaccgg ccccagatgg gggagcaggg ggaaggggac agccagccgc agggagagga
     1561 gcagccaggc agtacagcgc caccgggcgg cgtagcggcc ccgggtgaga cgggcagcca
     1621 gcagcaggag caggaccact gccccgccgc ccagggagag ggcggccaga tcaaggagaa
     1681 atcggttcat ttggcgcccc tttccagctc atccagcagg gccctgagct cgtccacctc
     1741 gccgggggcc agcccgtccc gggccagggc agccacgaag tttcgggggg aactgccgta
     1801 caggcgggag aggacggagc ggctgtcaaa ggagaggtag tcctctcggc tgaccagggg
     1861 ggtgtaccgg ttgctcctgc ccaggcggcg ggcggataga aaccctttgt ccgccagccg
     1921 ggtgagatag gtgttgatgg tgttggcggc ccagccgtgg ggggccagag cgcgctccag
     1981 ggcggcgcgg ggggtgtcgg ggccggagtc ccaaagggcc ttcatgacct ccagctcagt
     2041 gtcggggagt cgtttcacag ctatcgctcc aatctactac ttttgtagtt tgtaataata
     2101 tactacattt gaagtaaata aaggtcaagt tacagtgtgg ttacagagcg gccggggcag
     2161 ggctctggca gaacaaagca ggccgcctgc caaaaggcag acggcctgaa aatagggaaa
     2221 agcggtgccg cggaggggag cggggcacta aagcaggatc tgtccgttga tctccagccg
     2281 gaatttgagg tccagggtct cggcagcttt ccggctgagg agcatccgct ggagaaagat
     2341 ctcaaaatag tccatgacgg cgcacacctc cgtgttgatg gagagggaga gagtcacggt
     2401 ccgtcccggc aggtccagca gcacctcgga gcgctccacg gagtagttca cccggtcgtg
     2461 gatgtcaaag gtggacatgt ccttgttccg cacccggctg cggcgcacat cggtcttgtc
     2521 ggccaggatc agggcggcgg cgatggcgtt caccgggtag gcggtggagt cgtcgtggtg
     2581 gccgatggca gtgatgacgg cggccacgtc ggggggctcc atccccatct tgtccaggat
     2641 gcggaaggcc atggtggccc cgctgagggc gtggtcgtgg cggttgacca cgttgccgat
     2701 gtcgtgcatg taggcggcga tcctggccag ctacacctct ttcggggtgt agcccagctg
     2761 ggnnnnnnnn nncaggatgc gggcggccac ctcggcgcac ttggtcacat gggcgaagga
     2821 gtgctccgta aagcccagcg ccgtgagcga ggcgtccgcc tgggtgatgt aggtgcggat
     2881 ctccggagaa ctcctcacca tctcaaaggt aacattcata gggtgctcct ttcttctccg
     2941 ccgtcccaca ggcagcggga caggtccacc ctgccatcgg gcaggaaggg gacgccctct
     3001 ccttccagca gcagccgctg gatgcccgtt ccgccgaaca cgtccgcggg gcggaggccc
     3061 ccatcccggt acaggactcg atgacagggc aggccctccg gtgcccgggc cagggcgcag
     3121 cccacgatgc ggctggccct gggccggccg gccagaaagg ccaccagacc ataggtggcc
     3181 acctgtcctg ccggaatgcg ggccaccacg gcgtagaccg ccgtaaaaaa atcaggacgc
     3241 ccgggagtca cagggcacag cggcgggggc gccccgcttg ggcgggacgg gcccctctat
     3301 gggccgggca gaaaaacaga taaaacacag gtaccaacac tccatcgatc ataagcggcc
     3361 tggaagcagg tgctccccgc ccttgcaggt tggacaaaac agctggggac atccctattg
     3421 tatgggatga tcctggtaat ttcaagcccc aagagcatca ggacagaaaa tttctcctaa
     3481 gctgggcatc gttcccgcat gcggatgagg gaataggaga tcgttgcggg agaaacgggg
     3541 aaatggaaac gggctcccgt ggaaatgcct gatgcccgct gcatgtgcgg aagaaaaagg
     3601 ggaatttcca ccataacagg tggaagtgta tgctggacta tgatatagta gtttcaaaca
     3661 ggctgcgggc aggccgtccg gcgggcgctt tggagagggg gagacaggat gaagctttct
     3721 tcatttctgc attttgccgc ctttggggtc aggaccattc ttctgcggga aaaaaagccc
     3781 atcctgggca cagtgatcgt cacagaccgg tgcaatctga agtgcaggca ctgctctgtc
     3841 cacgatctga catcggtgga ccatccctat gaacagatcc gccgggagat ggagctcctc
     3901 taccatatgg gggtgcgcat cctgttcttc tgcggcggtg agaccttttt gtggcacagc
     3961 gacggccaca gcctgcggga cctggtgtgg gaagcaaagc agatgggctt tctgatcgta
     4021 aacgtggtca ccaacggta
//
LOCUS       DBMDSJ010000113         3981 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5891_length_3981_cov_1.927062, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000113 DBMDSJ010000000
VERSION     DBMDSJ010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5891_length_3981_cov_1.927062"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="ACZPAQ_03900"
     CDS             <1..1764
                     /locus_tag="ACZPAQ_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012515.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR0903158.1"
                     /translation="IKNTLNSKGFTLAVMLRSVASSSNLLKGVISGAKTKGYAVLLFD
                     SSCSQEQEDSNFRELSRHHVDGLLWEPVSCRTEERERELAEAHICLQMCSGSEPAWYQ
                     DNFRQFGRLMTQRLVNLSHTSIAFVKGENCVASSTLLEGYEQCLYENKIPYDAARVLE
                     VEGQLPEQISVGVSAFVCADPLSAQRVICRLTAAGFTVPGDASVIALASDVPCPSHEI
                     SLERVPYDTYGLALVDSLIARCEDRDEGQPPPAPAEELTSLATLARAFSARKKRVIVL
                     GSINADITLNVSQLPQIGSSVVSHNMSVNLGGKGANQAMGVAKLGWEACLIAKVGNDV
                     DATMAFQALNSEGVNTTGVRRELGSDTGKAYIHVQEDGESMMTLFPGANGTMTAEYVR
                     SMSHLFQNAGYCLLSTGPAPDCVLQAARLSKGHGAQTIFKPSSGVEIPEELYRLTDIF
                     VPNRHAAAKLAPFPEVERQAEYFLEQGVRNVIITLGHRGCYLRTPEQAFWYDAPPVAT
                     VDATGAADAFIAALAVYLLQNYSLEDAVEIASHAAGFCVTKQGVIPALVDRNSLDQYL
                     ATRPRPLRCSPSPLPASHKRR"
     gene            2019..3143
                     /locus_tag="ACZPAQ_03905"
     CDS             2019..3143
                     /locus_tag="ACZPAQ_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903159.1"
                     /translation="MSTVSLKNIYKIYDNSVTAVSDFNLEIADKEFIVLVGPSGCGKS
                     TTLRMVAGLEEISKGELYIDGKLVNDVEPKDRDIAMVFQSYALYPHMTVRENMEFPLK
                     LKKVPKEEIQQRVDQAAEILGITQYLDRKPKALSGGQRQRVAIGRAIVREPKVLLMDE
                     PLSNLDAKLRNQMRAEIIKLRQRINTTFVYVTHDQTEAMTLGDRIVIMKDGFVQQIGT
                     PQEVFEKPINIFVAGFIGTPQMNFFNARLEKKGGDYSVEYAGASFPLDGEMSAKLAGK
                     GQQSADVILGVRPEHIAVQKDPSASAIEASIEVSELMGSELYLHATVEGGQEVVIRTP
                     TVELPAEYRGGVPYGTKLRFTFSPSLIHLFDPESEKSLLV"
     gene            complement(3234..>3981)
                     /locus_tag="ACZPAQ_03910"
     CDS             complement(3234..>3981)
                     /locus_tag="ACZPAQ_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR0903160.1"
                     /translation="MEVVSVGVMYYAAIVPCVVSSLVSVQLARFFGLHIGTGYLIGNV
                     AVLGPWSMAQVCVMGVLCALLSILFCKVMHAAPKLYAKYFPNPLVRAAVGGALVLLLT
                     LLVGEQTYNGAGDAVIRRMLAGETISEAFLLKLVFTALTLGAGFRGGEIVPVLFTGAA
                     FGTVVGPLLGLSQSFGGALGMTAVFCGATNCPITSILLAYELFTSRGLPLYALCCAIS
                     YMLSGYYGLYSEQKIIYSKFKATWVDRKAE"
BASE COUNT          856 a         1173 c         1262 g          690 t
ORIGIN      
        1 atcaaaaata cactgaacag caagggattc accttggctg ttatgctacg cagtgttgcc
       61 tcctcctcta atctgctcaa aggcgtaatc tccggggcca agaccaaggg atacgcggtg
      121 ctactctttg atagctcctg ctcccaggag caggaggact ccaacttccg ggagctgtcc
      181 cgccaccatg tggacggcct gctgtgggag ccggtctcct gccgaacaga ggagcgggaa
      241 cgggaactgg cagaggcaca catctgtctt cagatgtgca gcggctctga gccggcctgg
      301 tatcaggaca atttccgtca gttcggtcgg ctcatgaccc agcggctggt aaacctgtca
      361 cacacctcca ttgcctttgt gaagggagag aactgtgtgg cctcctccac actgttggag
      421 gggtatgagc agtgcctgta tgagaacaaa atcccctatg atgcggcccg ggtgctggag
      481 gtggaagggc agctgcccga gcaaatctcc gttggggtca gcgctttcgt gtgtgccgac
      541 cccctttccg cccagcgggt gatttgtcgg cttactgcgg cgggcttcac cgtgccgggg
      601 gacgcatctg ttattgccct ggcctcggat gtaccttgcc cctcccatga gatatctctg
      661 gagcgggtgc cctatgacac ctatggcttg gcgctggtgg acagtctcat tgcccgatgc
      721 gaagaccggg acgagggaca gccgcccccc gcccctgcgg aggagctaac cagcttggct
      781 acgctggcgc gggccttttc ggcccggaag aaacgggtca tcgtcctggg cagcatcaac
      841 gcagatatca ccctgaacgt cagccagctg cctcagattg gcagcagcgt ggtgagtcat
      901 aatatgtccg tgaatttggg tggcaagggg gccaaccagg ccatgggcgt agcgaagttg
      961 ggatgggagg catgcctgat tgccaaggtg ggcaatgacg ttgacgccac catggccttt
     1021 caggcgctta acagtgaagg agtgaatacc accggggtcc gccgagagtt gggcagcgac
     1081 acaggaaagg catacatcca tgttcaggag gacggggaga gcatgatgac cctgttccca
     1141 ggggcaaacg gaaccatgac ggcggaatat gtcagatcta tgtcccattt gttccaaaac
     1201 gcggggtact gcctgctcag caccggacct gcgccggact gcgtgctcca ggccgccaga
     1261 ctgtccaagg ggcacggggc ccagaccatt tttaagcctt cctcgggggt ggaaatcccg
     1321 gaggagctgt atcgccttac cgacatcttc gtgcccaacc gccatgcggc ggccaaactg
     1381 gcccccttcc ctgaggtgga gcggcaggcg gaatattttc tggaacaagg agtgcgaaat
     1441 gtcatcataa ccctagggca ccggggctgt tacctgcgta ccccggagca ggcattttgg
     1501 tatgacgcgc ccccggtggc cactgtggac gccaccggag cggcagatgc cttcatcgcg
     1561 gcactggcgg tctatctact gcagaattac tctttggagg atgcggtgga aatcgccagc
     1621 catgcggcag ggttttgtgt caccaaacag ggggtcatcc cggcgctggt ggaccggaac
     1681 tccctggacc agtatctggc gaccagaccg agacctctgc gctgctcccc ctctccactt
     1741 cccgcttccc ataagagaag gtaaaggaag aacaacatcc atcgtgcggg aagcggcagg
     1801 gatgatctcg gctgccatcg ggaagtactg ctgatctaac gagaccaggg ccgtggaact
     1861 actccattct tcatagtcct cggacccata aaaaaggagg aagaaaagac cgccggccgg
     1921 cggtgcggca gtgtggagac ctgtcgtaag ccccgggatg gacagcccgt ccgggggagg
     1981 ggcctgctgc ggaggcagag gtctcacggc cagaagccat gtcaactgtc agtctgaaga
     2041 acatctacaa gatctatgac aactcggtca ccgcagtgag cgacttcaac ctggagatcg
     2101 cggacaagga gttcatcgtg ctggtgggcc cctccggctg cggcaagtcc accaccctgc
     2161 ggatggtggc gggcctggag gagatcagca agggagagct gtacatcgac ggcaagctgg
     2221 tcaacgacgt ggagcccaaa gaccgggaca tcgcgatggt gttccagagc tacgcactgt
     2281 atccccacat gacggtgcgg gagaacatgg aattccccct gaagctgaag aaagtgccca
     2341 aggaggagat ccagcagcgg gtggaccagg cggcggagat cctgggcatc acccagtatc
     2401 tggaccggaa gcccaaggcg ctgtccggcg gccagcggca gcgtgtggcc atcgggcgag
     2461 ccattgtccg ggagcccaag gtgctgctga tggatgagcc cctgtccaac ctggacgcca
     2521 agctgcgcaa ccagatgcgt gcggagatca tcaagctgcg gcagcggatc aacaccacct
     2581 tcgtgtacgt gacccacgac cagacagagg ccatgaccct gggcgaccgg atcgtgatca
     2641 tgaaggacgg ctttgtgcag cagatcggca ccccccagga ggtctttgag aagcccatca
     2701 acatcttcgt ggccggcttc atcggtacgc cccagatgaa cttcttcaac gcccgcctgg
     2761 agaagaaggg cggcgactac tcagtggagt atgccggggc gagcttcccg ctggacgggg
     2821 agatgagcgc caagctggcc gggaagggac agcagagcgc cgacgtgatc ctgggcgtgc
     2881 gtccggagca catcgcggtg cagaaggacc cttccgcctc cgccatcgag gcatccatcg
     2941 aggtgtccga gttgatgggc agcgagctgt acctccacgc caccgtggag ggcggccagg
     3001 aggtggtcat ccgtaccccc accgtagaac tgccggcgga gtaccggggc ggcgtgccct
     3061 atggcaccaa gctccgcttc accttctccc ccagcctcat ccatctcttc gaccccgaat
     3121 ctgagaagag cctgctcgtc tgaaaaaact tttttggaaa cagaagcccc cctgtgccgg
     3181 cctgaggacc ggcacagggg ggtgatggta tttcgggaga cagacgggag aggctactcc
     3241 gcctttcggt ccacccaggt ggccttgaat ttggagtaga tgatcttctg ctcgctgtaa
     3301 aggccgtagt acccggagag catgtaggag atggcacagc acagggcgta aagaggcagg
     3361 ccccggctgg taaatagttc ataggccagg aggatggagg tgatggggca gttggtagcg
     3421 ccgcagaata ccgccgtcat cccaagcgcc ccgccgaagg actgggacag acccagcagc
     3481 ggtccgacca ccgtgccaaa ggcggcacca gtgaagagca ctggcacgat ctcgccgccg
     3541 cggaagccgg cccccagagt gagggcggta aagaccagct tgagcaggaa cgcctccgag
     3601 atggtctcgc ccgccagcat ccggcggatg acagcatccc cggcgccgtt gtaggtctgt
     3661 tcccccacca gcagggtcag cagcagcacc agggcgccgc caacagccgc ccggaccagg
     3721 ggattgggga agtatttggc atagagcttg ggggccgcat gcatcacttt gcagaacagg
     3781 atggagagca gagcgcacag cacccccatc acgcacacct gggccatgga ccagggaccc
     3841 aggacagcca catttccgat gagatagcct gttcctatat gaaggccgaa gaagcgggcc
     3901 agctggaccg agaccaaaga ggagacgaca caggggacga tggcggcgta gtacatcact
     3961 cccacgctca ccacctccat g
//
LOCUS       DBMDSJ010000114         3969 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5915_length_3969_cov_1.796019, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000114 DBMDSJ010000000
VERSION     DBMDSJ010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5915_length_3969_cov_1.796019"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="ACZPAQ_03915"
     CDS             complement(<1..250)
                     /locus_tag="ACZPAQ_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR0903161.1"
                     /translation="MPDVDMRVELAGVTLKNPVCVASGTFGFGREYGAFYDLGELGAI
                     CAKGLTLHRREGNPGPRIAETPMGILNSVGLQNPGVDAF"
     gene            complement(243..1028)
                     /locus_tag="ACZPAQ_03920"
     CDS             complement(243..1028)
                     /locus_tag="ACZPAQ_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR0903162.1"
                     /translation="MKVEQKCGIISRTMLNDAAISLTLEVGDMVRTAFRAPGQFVHIK
                     CGHSRLLRRPISVCACQAGEGDAPDRLTVVFEVRGEGTAWLAGRREGQSLDVMGLLGN
                     GFPIEREGRYLLVGGGIGVPPMLGCAQYAPGRCTAILGFRSGDRVMLEEQFRSVCSQV
                     QVATDDGSYGFHGYVDALVRRELEGDRGYDAVLACGPRPMLRNVARAAEDFGVRCLVS
                     MEERMGCGVGACLVCACDMADGSRKHVCKDGPVFEAREVDWDA"
     gene            complement(1088..1564)
                     /locus_tag="ACZPAQ_03925"
     CDS             complement(1088..1564)
                     /locus_tag="ACZPAQ_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903163.1"
                     /translation="MAKRACVLCGEMAGSWSLCYITLCGTSQTLCEKCMRRYRDAAGE
                     EKIALREYMARSPHLREGEEVRERLPQARVQWQRELEQARERRQQEEQRQAQVKKTAR
                     CCGQDMTKLGVSTFQLGEHTFLGGDLDHLLSGSMKMAVFRCEVCGRLKFFDPEWVQ"
     gene            complement(1608..2531)
                     /gene="pyrF"
                     /locus_tag="ACZPAQ_03930"
     CDS             complement(1608..2531)
                     /gene="pyrF"
                     /locus_tag="ACZPAQ_03930"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR0903164.1"
                     /translation="MSFDVLQDKIRAMKNPTVAGLDARIDYVPEHIRQAAFAEHGTGL
                     EGACEAIYQFNVGLIDALCDIVPAVKPQSAYYENLGWRGMEMLERTIRYAKSKGLFVI
                     ADIKRGDIGSTASAYAEGWLSGVKIGEETFKTFDADCVTLNGYMGSDSINPFLKAAQG
                     EDKCVFVLVKTSNPGSGELQDMVAGDRLVYQVMGDLNQRIAKGTEGKYGYTMAGAVTG
                     ATYPSDIRALRKRLEHTFFLVPGYGAQGGTAEDVQYAFDKYGHGAIVNSSRGIMCAWQ
                     KTGGDGRDFAQAARDAAIAMRDDIRRFVTIV"
     gene            complement(2618..2983)
                     /locus_tag="ACZPAQ_03935"
     CDS             complement(2618..2983)
                     /locus_tag="ACZPAQ_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903165.1"
                     /translation="MPTLFFVMGNLLIIGASAQLIRLGYIPLPGLIAAFFWGETWVFW
                     GITDLLGWPWRLKRSVREAPWRKEYQRRIGPIQIITGGLIMPASLLELMPAFLLSLLV
                     TVAGGLAAYNIRCQYPGSW"
     gene            complement(3057..>3969)
                     /locus_tag="ACZPAQ_03940"
     CDS             complement(3057..>3969)
                     /locus_tag="ACZPAQ_03940"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR0903166.1"
                     /translation="IAAVSVGLRGEEPTDAEALKKAGAVALSDDGCNVDNANLMRDAM
                     IRAKRQELPVLCHCEDTSMVEGRAVNEGSVSRQLWLEGRPAIAEELEVMRAAMLAEET
                     GARVHICHISTAKAVDIVRRMKEHGVPVTCETCPQYFTLTEDEILTQGSLARINPPLR
                     TQQDVKGIIAGLRDGTIDAIATDHAPHSAEEKARPLTRAPSGMVGLETSLAVTLTALY
                     HTGKMELTEIVRRMTVNPASILGLSKGRLALGNDADIVIFDPNEEWTVDPEQFASKGR
                     NTPFAGWKVKGRVKYTIVGGTVIYEAP"
BASE COUNT          650 a         1419 c         1162 g          738 t
ORIGIN      
        1 caaaggcgtc cacccccggg ttctggaggc ccaccgagtt gagaatgccc atgggagtct
       61 ctgcgattcg ggggcccggg ttgccctccc ggcggtggag ggtcaggccc ttggcgcaga
      121 tggcgcccag ttcgcccagg tcatagaacg ccccgtactc ccggccgaag ccgaaggtcc
      181 cagaggccac gcacaccggg tttttcagag tcaccccagc cagctccacc ctcatatcca
      241 catcaggcat cccaatccac ctccctggct tcaaagacgg gtccgtcctt gcacacatgt
      301 ttccggctgc cgtccgccat gtcgcaggca cagaccaggc aggcgcctac gccgcagccc
      361 atccgctcct ccatggacac caggcagcgc acgccgaagt cctccgccgc ccgggccacg
      421 tttctcagca tgggtcgggg gccgcaggcc agcaccgcgt catagccccg gtctccctcc
      481 agctcccgcc gcaccagcgc atccacatag ccgtggaagc cgtaggagcc gtcatctgtg
      541 gccacctgca cctgggagca cacggagcgg aactgctcct ccagcatcac cctgtccccg
      601 gagcggaacc ccaggatggc ggtgcaccgc ccgggggcat actgggcgca gcccagcatg
      661 gggggcacgc cgatgccgcc ccccaccagc agatagcggc cctcccgctc tatggggaag
      721 ccgttgccca gcaggcccat cacgtccagg ctctgtccct cacggcggcc ggccagccag
      781 gcggtcccct ctccccggac ctcaaagacc acggtcagcc ggtcgggggc gtccccctcc
      841 cccgcctgac aggcgcagac ggagatgggc cgacggagca gccgggaatg tccgcacttg
      901 atgtggacga actgccccgg ggcccggaag gcggtccgga ccatgtcccc cacctccaga
      961 gtgaggctga tggcggcgtc attgagcatc gtcctgctga tgatgccgca tttctgttct
     1021 actttcatta tggcgtcaca ccctctgtgg aaagtttagt gtctcgcggg cacacctggg
     1081 gcggccctta ttggacccat tccgggtcaa aaaatttcag ccggccgcag acctcgcagc
     1141 ggaacaccgc cattttcatg ctgcctgaca gcagatggtc caggtcaccg cccaaaaagg
     1201 tgtgctcccc cagctgaaag gtggataccc ccagcttggt catgtcctgg ccgcagcagc
     1261 gggcggtctt tttcacctgg gcctggcgct gctcctcctg ctgccggcgc tcccgcgcct
     1321 gctccagctc ccgctgccac tggacccggg cctgggggag gcgctcccgc acctcctccc
     1381 cctctctcag gtgaggggac cgggccatat actccctgag ggcgatcttc tcctcccccg
     1441 ccgcatcccg gtatcgccgc atgcacttct cgcacagggt ctgactggtg ccgcacaggg
     1501 tgatgtagca cagggaccag cttcccgcca tctctccgca gagcacacag gcccgctttg
     1561 ccatagtcct tcctccttcc cccagccccg cccttttgcg ggagctgtta cacgatggtg
     1621 acgaagcgcc tgatgtcgtc ccgcatggcg atggccgcat cccgggccgc ctgggcaaaa
     1681 tcccggccgt ccccgccggt cttctgccac gcacacatga tgccccggga ggagttgacg
     1741 atggccccgt ggccgtactt gtcaaaggca tactgtacgt cctccgcagt ccccccctgg
     1801 gcgccgtagc cgggcaccag gaagaaggtg tgctccaggc gcttgcgcag ggcccggatg
     1861 tcggaggggt aggtggcccc ggtgacggcc ccagccatgg tatagccgta cttgccctcg
     1921 gtccccttag cgatccgctg attcaggtcg cccatcacct gatagaccag ccggtcccca
     1981 gccaccatat cctggagctc tccggagccg ggattggagg tcttgaccag cacgaagaca
     2041 cacttgtcct ccccctgagc tgccttcagg aaggggttga tagagtcaga gcccatataa
     2101 ccgttgaggg tgacgcagtc ggcgtcaaag gtcttgaagg tctcctcccc gatcttcacc
     2161 ccggacagcc agccttccgc gtaggcggag gcggtggaac cgatgtctcc ccgcttgatg
     2221 tcggcgatga cgaacagccc cttgcttttg gcatagcgga tggtgcgctc cagcatctcc
     2281 atcccccgcc agcccagatt ttcatagtag gcggactgtg gcttcactgc ggggacgatg
     2341 tcgcacaggg cgtcgatcag ccccacgttg aactgataga tggcctcaca ggctccctcc
     2401 agcccggtgc cgtgctccgc gaaggcggcc tggcggatgt gctcgggcac atagtcgatc
     2461 cgggcgtcca gcccggccac tgtagggttc ttcatggccc ggatcttgtc ctggagaacg
     2521 tcaaaagaca tcaaatatca ttccttttcg ttattttcat ctttatttct actatttgtt
     2581 ttgcattttg ggggcctggc ccctcttccg gccggtctca ccagcttccc ggatactgac
     2641 agcggatatt atatgcggcc aggccccccg ccacagtcac caacagggac agcagaaagg
     2701 ccggcatgag ctccagcaga gaggctggca tgatgagccc ccctgtgatg atctgaatgg
     2761 gcccgatccg ccgctgatac tctttcctcc acggggcctc ccgcaccgac cgtttcagcc
     2821 gccagggcca tcccagcagg tctgtgatcc cccagaagac ccaggtctct ccccaaaaga
     2881 aggccgcgat cagtcctggg agagggatat atcccagccg gatcagctgg gccgatgccc
     2941 ctatgatcag gagattcccc atcacaaaaa acagcgtcgg cacccagctg ggcacgctcc
     3001 tccgcgtctt catgccgatc ccacccttct gctccccctc cgccgcaggc cggctctcag
     3061 ggggcctcgt agatgaccgt tccgcccacg atggtgtact tcacccgccc ctttaccttc
     3121 catccggcga agggggtgtt gcgccccttg gatgcaaact gctccgggtc taccgtccac
     3181 tcttcgttcg ggtcgaagat gacgatgtcc gcgtcatttc ccagagccag ccgtcccttc
     3241 gacagcccca ggatagaggc ggggttcacc gtcatccggc ggacgatctc cgtcagctcc
     3301 atctttccgg tgtgatacag ggcggtcagg gtgactgcca gggaggtctc cagcccaacc
     3361 atgccgctgg gcgcccgggt gaggggcctg gccttctcct cggcggagtg gggggcgtgg
     3421 tcggtggcga tggcgtcgat ggtgccgtcc cgcaggccgg cgatgatccc cttcacatcc
     3481 tgctgtgtcc gcaggggggg attgatcctg gccagggacc cctgcgtcag gatctcgtcc
     3541 tctgtgaggg taaaatactg ggggcaggtc tcgcaggtaa cgggcacccc gtgctccttc
     3601 atccggcgca cgatgtccac cgcctttgcc gtcgagatat ggcagatgtg gacccgggcg
     3661 ccggtctcct ccgccagcat ggccgcgcgc atcacctcca gctcctcagc gatggcgggc
     3721 cgtccctcca gccagagctg gcgggatacg ctgccctcgt tcaccgcccg gccctccacc
     3781 atggaggtgt cctcacagtg gcagaggaca ggcagctcct gccgcttggc ccttatcatg
     3841 gcgtcccgca tcaggttggc gttgtccacg ttgcagccgt catcggagag ggccaccgcc
     3901 cccgcctttt tcagcgcctc tgcgtcggtg ggctcctccc ctctcagccc cacggacacc
     3961 gccgcgatg
//
LOCUS       DBMDSJ010000115         3944 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5954_length_3944_cov_1.695030, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000115 DBMDSJ010000000
VERSION     DBMDSJ010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5954_length_3944_cov_1.695030"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="ACZPAQ_03945"
     CDS             complement(<1..569)
                     /locus_tag="ACZPAQ_03945"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR0903167.1"
                     /translation="MAEQRQDRRVRRSKAQLRQALIQLLQEKPVEEITVRELTERADV
                     NRGTFYSHYQNIYHMLEQVENELFDELGRLLDAYPPHLLRDELTPILVDVFRFVRSNS
                     QLCLALMGAWGEGDFFRRLDAMIYARCLREWQGIYPLGSLDGPNYCLEFVIAGAVGMA
                     RSWALQGFPQAEEELARLADRLISSGLAGT"
     gene            731..1432
                     /locus_tag="ACZPAQ_03950"
     CDS             731..1432
                     /locus_tag="ACZPAQ_03950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903168.1"
                     /translation="MAYISLQHVGKRYQMGEITITAVDDISFEIEKGQFAVIVGPSGA
                     GKTTVLNILGGMDACDSGAIRVDGQEISGYGARQLTTYRRYDIGFVFQFYNLVQNLTA
                     LENVELAAQICRDPLDAEEVLRHVGLGERLNNFPAQLSGGEQQRVAIARALAKNPKLL
                     LCDEPTGALDYVTGKSILKLLQDTCRQQGMTVVVITHNSAIAPMADQVIHVKNGRVER
                     IDHNDRPVPVETIEW"
     gene            1495..>3944
                     /locus_tag="ACZPAQ_03955"
     CDS             1495..>3944
                     /locus_tag="ACZPAQ_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR0903169.1"
                     /translation="MSRKKAPSRLLTDAVREVRNTRSRFLSLLVLSALAVCFLAGLRA
                     TAPDMKETGDLYFDQQRLMDLRVVSTLGLTEEDVELLARQEGVEEAEGAYTIDAAVPL
                     KDQEMIVKVHSFAEEGSNRPRLVEGRLPRKAGECLVEPLFLEKSGLSIGDTIRLDTGT
                     GNFEDALARREFTIVGTADSPLYISVERGSSSLGTGAVSAFVLLPQAAFSLDYYTDAY
                     LRAEGAEELLCYSEDYTELVNSLSDALEPLAEERSALRGESIREEAEQALADARRELS
                     DGEAEVSQELSEGEAELTDARRELDDGWAEYYDGLNTFEQETADAQREIDDGEAELAD
                     ALTELRDGEQEYQDALPELEDARREVADARQELADARAELDDGWAEYRKGYAKYEYGL
                     AAYTEGMAQYEQGLAGVEQMRAGAQQARQGAAQAQQGAVQAQQGAAQAGAAAQQYAAQ
                     AQQAAAMGNLLLAQQLQAQAQQYEAQAQQLTAQAQQLQAQAAQALAQAEALEVQAVQV
                     EAQLSDGLPRLQSSALELNASLGLLTQAKRELDDGEAEYADGAARLADGEQELADGER
                     ELADARQELDEGWQEYRDGQAELADARETLERESEDGRAELADALTELQNGEAEYADG
                     LADYEEGKAEAEQELADARKELEQARRDLDALEDCSWYLLGRGTNTGYASYEMDADRM
                     GSLAAVFPLIFFLVAALVCLTTMTRMVEEQRITIGGMKALGYSKGAIAVKYVGYGFLA
                     SAAGAALGLAVGLTLLPWIICNAWAILYTVGEIQYGFYPATSLLACGAAVGTVTLSAL
                     AACFSTLAAVPAELMRPKA"
BASE COUNT          717 a         1212 c         1416 g          599 t
ORIGIN      
        1 gtgccggcga gaccagagga gatgagccga tcggccaggc gggccagctc ctcctctgcc
       61 tgaggaaagc cctgaagggc ccaggagcgg gccatgccca ccgccccggc gatgacgaac
      121 tccagacagt agttggggcc gtccagactg cccagggggt agatgccctg ccactcccgc
      181 aggcagcggg catagatcat ggcgtccagc cggcggaaga agtcgccctc cccccatgcg
      241 cccatcaggg ccagacacag ctggctgttg ctccgcacga agcggaacac gtccaccagg
      301 atgggggtca gctcgtcccg gagcaggtgg ggcggatagg catccagcag ccgccccagc
      361 tcgtcgaaga gctcgttttc cacctgttcc agcatgtggt agatgttttg atagtggctg
      421 taaaaggtcc cgcggttcac atcggcccgt tcagtcagct cccggacagt gatctcctcc
      481 accggctttt cctggagcag ctggatcagg gcctgccgca gctgggcctt ggacctgcgg
      541 accctccggt cctggcgctg ttccgccatg gacgcacctc ctccgttttt cgacacagag
      601 gacagaacct gtcggatttt ccacagaagc cgcccttttg cccattgcaa tcctctgtta
      661 tcccctatta taataaaaaa tacaagatat tcaacacctg ttttataacg gacaacagga
      721 gggatgacct atggcctata tctcccttca gcatgtgggg aaacggtatc agatggggga
      781 gatcaccatc accgcagtgg acgacatctc ctttgagatt gagaaaggac agttcgccgt
      841 catcgtgggg ccctccggcg cgggcaagac cacggtgctg aacatcctgg gggggatgga
      901 cgcctgtgac agcggggcta tcagggtgga cggccaggag atcagcggct atggggcccg
      961 acagctcacc acataccgcc gatatgacat cggcttcgtg ttccagttct acaacctggt
     1021 gcagaatctg acggccctgg aaaatgtaga gctggccgcc cagatctgcc gggaccccct
     1081 ggacgcggag gaggtgctgc gccatgtggg gctgggggag cggctgaaca actttcctgc
     1141 ccagctctcc ggaggggagc agcagcgggt ggccattgcc cgggcgctgg ccaaaaaccc
     1201 caagctgctg ctgtgcgacg agcccacggg ggcactggac tatgtcaccg gcaagtctat
     1261 cctgaagctg ctccaggaca cctgccgcca gcagggcatg acggtggtgg tcatcaccca
     1321 caacagcgcc atagccccca tggcagacca ggtgatccac gtgaagaacg ggcgggtgga
     1381 gcgcatcgac cacaacgacc gcccggttcc cgtggagacc attgagtggt aatgcctcta
     1441 caaaatgcga ttttgtgaag aaaaaataca aaaaggagag gaggggaaca gcccatgtcc
     1501 cgaaaaaaag cccccagccg cctgctgacc gacgcggtcc gggaggtccg gaacacccgc
     1561 agccgcttcc tctccctgct ggtcctctcc gccctggcgg tgtgcttcct ggccggactg
     1621 cgggccactg cccccgacat gaaggagacc ggtgacctgt attttgatca gcagcggctg
     1681 atggacctgc gggtggtctc caccctgggc ctgacggagg aggatgtgga gctgctggcc
     1741 cggcaggagg gggtggagga agccgagggg gcctacacca tcgacgcagc tgtccccctg
     1801 aaggatcagg agatgatcgt aaaggttcac tcctttgcag aggaggggtc caaccgcccc
     1861 cggctggtag aggggcggct gccccggaaa gcgggggagt gcctggtgga gcccctgttt
     1921 ctggagaagt cgggcctgtc catcggggac accatccgcc tggacaccgg gaccggcaac
     1981 tttgaggacg ccctggcccg gcgggaattc accatcgtgg gaacggcgga ttcccctctg
     2041 tatatctccg tggagcgggg ttcttcctct ctggggacgg gggcggtctc agcctttgtt
     2101 ctgctgccgc aggcggcctt ttccctggac tactacaccg acgcctacct tcgggcggag
     2161 ggggcggagg agctgttgtg ttacagtgag gactacaccg agctggtgaa cagcctttcc
     2221 gacgccctgg agcctctggc ggaggagcgc tccgccctgc ggggggagtc catccgggaa
     2281 gaggccgagc aggccctggc ggacgcccgg cgggagctgt ctgacgggga ggcggaggtg
     2341 tctcaggagc tctccgaggg ggaggcggag ctgacggatg cccggcggga gctggatgac
     2401 ggctgggcgg agtactatga cgggctgaac acctttgaac aggagaccgc cgatgcccag
     2461 cgggagatcg acgacgggga ggcggagctg gcggacgctc tgactgagct gcgggacggg
     2521 gagcaggagt accaggatgc cctgcccgag ctggaggatg cccgccggga ggtggcggac
     2581 gcccggcagg agctggcgga cgcccgggcg gagctggacg acggctgggc ggagtaccgc
     2641 aaggggtacg ccaaatatga atacggcctg gccgcctaca ccgaggggat ggcccagtat
     2701 gagcaggggc tggcgggcgt ggagcagatg cgcgccgggg cccagcaggc ccgccagggg
     2761 gcggcccagg cccagcaggg ggcggtccag gcccagcagg gggccgccca ggccggtgcg
     2821 gcggcccagc agtatgcggc ccaggcccag caggccgcag ccatgggaaa tctgctgctg
     2881 gcccagcagc tccaagccca ggcccagcag tacgaggccc aggcccagca gctgacggct
     2941 caggctcagc agctccaggc ccaggcggcc caggccctgg cccaggcgga ggcactggag
     3001 gtccaggccg tccaggtgga ggcccagctg tccgacggac tgccccggct ccagtccagc
     3061 gccctggagc tcaacgcctc cctggggctg ctgacccagg ccaagcggga actggacgac
     3121 ggggaggcgg agtacgccga cggcgcggcc cggctggcgg atggggagca ggagctcgcc
     3181 gacggggaac gagagctggc ggatgccagg caggagctgg acgagggctg gcaggagtac
     3241 cgggacggcc aggcggaact ggcggacgcc cgggagaccc tggagcggga gtcggaggac
     3301 ggccgggcgg agctggcgga tgccctgact gagctgcaga acggcgaggc ggagtatgcc
     3361 gacggcctgg ccgactatga ggaggggaag gcggaggcgg agcaggagct ggcggacgcc
     3421 cgcaaggagc tggagcaggc ccggcgggac ctggatgcct tggaggactg ctcctggtat
     3481 ctcctgggac ggggcaccaa caccggctac gccagctacg agatggatgc cgaccggatg
     3541 ggcagtctgg cggcggtgtt ccccctgatc ttcttcctgg tggccgccct ggtgtgcctg
     3601 accaccatga cccggatggt ggaggagcag cggatcacca tcggcgggat gaaggccctg
     3661 ggctactcca agggggccat cgcggtgaaa tatgtgggct acggcttcct ggccagcgcg
     3721 gcgggggcgg ccctggggct ggcggtgggg ctgaccctgc tgccctggat catctgcaac
     3781 gcctgggcca tcctctacac agtgggggag atccagtacg gcttttaccc cgctacctcc
     3841 ctgctggcct gcggggccgc ggtggggacg gtgaccctct ccgccctggc ggcctgcttc
     3901 tccaccctgg cagcggtgcc ggcggagctg atgcggccca aggc
//
LOCUS       DBMDSJ010000116         3925 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_5981_length_3925_cov_1.689331, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000116 DBMDSJ010000000
VERSION     DBMDSJ010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5981_length_3925_cov_1.689331"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="ACZPAQ_03960"
     CDS             <1..742
                     /locus_tag="ACZPAQ_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR0903170.1"
                     /translation="AALLIGVYTLGFVLPFLAVGLFTGGVLSFFKRHKQVVRWTGKAG
                     AALLILMGVMTFTGFMNGFTEYLSQTGSAVPQSQSVGSGEEPSASTDADNGASEPPVV
                     PAPDFTLVDQYGEEHSLSDYKGKTIFLNFWATWCGPCRGEMPDIQALYEEYGGNQGDL
                     VVLGVAAPGLGQEGTQEEIAIFLEENGYTFPTVMDTEYQLTYQYGIRAYPTTWMIDGE
                     GSLYGYVESAMTGEIMRDIVEQTMEAQP"
     gene            complement(807..2708)
                     /locus_tag="ACZPAQ_03965"
     CDS             complement(807..2708)
                     /locus_tag="ACZPAQ_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HIR0903171.1"
                     /translation="MRSVTSCFNSTLYRKNLSRFWPIWALYSVIWLFALPLNLLTTIQ
                     RGQNWALEDLTHSLDYFSRVSVGYSLNLGTVLAVIFGLLAAMAVFSYLDASRSACMMH
                     ALPLRREALFFTNYLSGLSFLLLPNAAIFGLTLAGEAVGNCLDLKALAIWLLVQSASC
                     LFFYSFAVFCAMFTGHLLALPVFYGVLNCLAAIISTLMDSVLYLFLYGYNGLSGTTQQ
                     AVAWLTPLEKLGGAVSWTQAADSGAYQLAQPAVVAVYAGVGVALSAAALLIYRARQME
                     SAGDVVSVRVVRPIFKYGVAVCAGLSGGTFTYYVLFGDGQLSLVLSILFWAAVGYFLA
                     EMLLRKSFRVFSAWPGCLVLLALLSALFLCIRLDLTGYQDYIPASGEVSQVTVDLEGS
                     TYPSDGFGGSVQITDPEQVAQITALHQAIVAARSREDSIYDDSFYISLSYTLSNGQSI
                     RRYYHGVPLYRAEQGMEGTVTWALDQLVSDRSFVRAGYGLDQSADWSLTEAYVYDLWD
                     IQTQSHESASLSAAQAEQLWQAVLTDFDAGTIGTRYLFEDQDRLENTCEADLDFSFYL
                     TEQDSMVHRGGSVTITLTPQASHTLAVLEELGVLTDTRIARTHAQADRWYAMTDGAED
                     TSSLYTASP"
     gene            complement(2683..3585)
                     /locus_tag="ACZPAQ_03970"
     CDS             complement(2683..3585)
                     /locus_tag="ACZPAQ_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903172.1"
                     /translation="MIKVNNVVKTFDGFRALDGLTMEVEKGSIYGLVGPNGAGKSTIL
                     RHITGIYRPDSGTITVDGAPVYENPAVKSRVAFIPDDLYYFLSASTRDMMKFYRGLYP
                     AFDDKRYQVLKEAFPAVDEKQPIRRLSKGMQKQSAFWLALCCNPDLLVLDEPVDGLDP
                     VMRRQVWSLLMGDVAQRGTTVLVSSHNLRELEDVCDHVGILSRGKVLLQRSLTDLQDN
                     VVKMQIVFQEPEMPKLPEDLDLLHVTQVGRIHTLIVRGSSDVIKSRLAAFQPILMEAL
                     PLTLEEIFIYELGGEDYAVRDIML"
     gene            complement(3582..>3925)
                     /locus_tag="ACZPAQ_03975"
     CDS             complement(3582..>3925)
                     /locus_tag="ACZPAQ_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0903173.1"
                     /translation="RPIYEQVRDNLRRLMVSGAIQEGEKLPSVRSLASNLAINPNTIQ
                     RAYESLEAEGYVYSIPGKGSFAAPRTGVDEERRDRLLGQFDSLTAELLYLGVTRDRLI
                     ARIREKGGEVQ"
BASE COUNT          779 a         1176 c         1325 g          645 t
ORIGIN      
        1 agcggcgctg ctcatcgggg tgtacaccct gggctttgtg ctgcccttcc tagcggtggg
       61 gctgttcacc ggcggggtgc tgtccttctt caagcggcac aagcaggtgg tgcgctggac
      121 ggggaaggcg ggggcggctc tgctgatcct gatgggggtg atgaccttta ccggctttat
      181 gaacggcttc accgaatatc tgtcccagac cggctccgcc gtgcctcaga gccagagcgt
      241 cggcagcggg gaggagccgt ccgcctccac agatgcagac aacggtgcgt cggagccgcc
      301 ggtggtccct gcccctgact tcaccctggt ggaccagtac ggggaggagc acagtctgtc
      361 cgattacaag ggcaagacca tttttctcaa tttctgggcc acttggtgcg gcccctgccg
      421 gggggaaatg ccggatatcc aggcgctgta tgaggagtat ggcgggaacc agggggacct
      481 ggtggtactg ggagtggccg cccctgggct ggggcaggag gggacccagg aggagatcgc
      541 catctttctg gaagagaacg gctatacctt ccccacagtg atggacacgg agtaccagct
      601 cacctatcag tacggcatcc gggcctaccc caccacctgg atgatcgacg gggagggcag
      661 tctctacggc tatgtggaga gcgccatgac aggggagatc atgagggata tcgtggaaca
      721 gaccatggag gcccagccat aaaaaagaaa catgccccgc ccgctccaat gggagcgggc
      781 ggggcatgtt tgactgtgtg ggtccatcag ggggaggcgg tgtagaggga agaagtgtcc
      841 tcagctccat ccgtcatggc gtaccagcga tcggcttggg catgggtgcg ggcgatgcgg
      901 gtgtcggtga gaacgcccag ctcctccaga acggccaggg tgtgggaggc ctggggggtg
      961 agggtgatgg tcacagagcc gccccggtgc accatgctgt cctgctccgt caggtagaag
     1021 ctgaagtcca ggtcagcctc acaggtgttc tccaggcggt cctggtcctc aaaaaggtag
     1081 cgggtgccga tggtgcccgc gtcgaaatcg gtcagcacgg cctgccacag ctgctccgcc
     1141 tgggcggcgg agagggaggc ggactcgtgg ctctgggtct ggatgtccca gagatcgtag
     1201 acgtacgcct ccgtcaggct ccagtccgcg gactggtcaa gtccatagcc ggcccggaca
     1261 aagctccggt cagagaccag ctgatccaga gcccatgtga cggtgccctc catcccctgc
     1321 tccgcccggt acagcggaac gccgtggtag tagcggcgga tgctctgtcc gttggacagg
     1381 gtgtaggaga gggagatgta gaaggagtcg tcatagatgc tatcctccct ggagcgggca
     1441 gctacgatgg cctggtggag ggcggtgatc tgggcgacct gctccgggtc ggtgatctgg
     1501 acgctcccgc cgaagccgtc gctgggatag gtgctgccct ccagatccac agtcacctgg
     1561 ctcacctcgc cggaggcggg gatgtagtcc tgatagccgg tgagatccag ccggatgcac
     1621 aggaacaggg cggagagcag cgccagcagg accaggcagc ccggccaggc ggagaagacg
     1681 cggaaggact tgcgcagcag catctccgcc agaaaatagc ccactgcagc ccagaagagg
     1741 atggacagca ccagggagag ctggccgtct ccgaacagca catagtaggt aaaggtgccg
     1801 ccggagagac cggcgcagac ggccaccccg tacttgaaga tggggcggac cacccgcacg
     1861 gagaccacgt ctcccgccga ctccatctgg cgggcgcggt agatcaacag ggcggccgca
     1921 gacaaggcca cccccacacc ggcgtagacc gccaccacgg cgggctgggc aagctgatag
     1981 gccccgctgt ccgcagcctg ggtccaggat acagcaccgc ccagtttctc cagaggggtg
     2041 agccaggcca cagcctgctg ggtggtgccg ctcaggccgt tgtagccgta gagaaacagg
     2101 tagagcaccg agtccatcag cgtgctgatg atagccgcca ggcagttcag caccccgtag
     2161 aagacaggca gggccagcag atggccggtg aacatggcac agaagacggc aaaggagtag
     2221 aagaagaggc aggaggcgga ctggaccagc agccatatgg ccagcgcctt cagatccaga
     2281 cagttcccca cagcctcgcc ggccagggtg aggccaaaga tggcggcgtt gggcagcagc
     2341 aggaagctga gcccggacag atagttggtg aaaaacagag cctcccgccg caggggcagg
     2401 gcgtgcatca tgcaggcgga gcggctggca tccagatagc tgaacaccgc catagcggcc
     2461 agcaggccaa agatgacggc cagcaccgtg cccaagttca gggagtagcc caccgagacc
     2521 cgggaaaaat agtccaggct gtgcgtaagg tcctccagag cccagttctg cccccgctgg
     2581 atcgtggtga gcagattgag gggcagggca aacagccaga tcaccgagta cagggcccag
     2641 atgggccaga agcggctgag gtttttgcgg tagagggtgg aattaaagca tgatgtcacg
     2701 gaccgcatag tcctcacctc ccagttcata gataaaaatc tcctccagcg tcagaggcag
     2761 ggcctccatc aggatgggct ggaacgcggc gagccggctt ttgatgacgt cggagctgcc
     2821 ccggacgatc agggtgtgga tgcggcccac ctgggtgaca tggagcaggt ccaggtcctc
     2881 cggcagcttg ggcatctccg gctcctgaaa tacgatctgc atcttgacca cgttgtcctg
     2941 aagatcggtg agggagcgct gcaggagcac cttgccccgg ctgaggatgc ccacgtggtc
     3001 gcacacgtct tccagttccc gcagattgtg ggaggagacc agcaccgtgg tcccccgctg
     3061 ggccacgtcc cccatgagca gggaccagac ctgacgccgc atcaccgggt ccaggccgtc
     3121 taccggctcg tccaagacca gcaggtcggg gttgcagcac agagccagcc aaaaggcgga
     3181 ctgcttctgc atccccttgg acaggcggcg gatgggctgt ttctcgtcta ctgccgggaa
     3241 ggcctccttc agcacctgat agcgcttgtc atcaaaggcg ggatacagcc cccggtaaaa
     3301 cttcatcatg tcccgggtgg aggcggacag aaagtagtac aggtcgtcgg ggataaaggc
     3361 cacccgggac ttcaccgccg gattttcgta caccggcgcc ccgtccaccg tgatggtgcc
     3421 cgagtcgggg cggtagatgc cggtgatgtg ccgcaggatg gtggactttc ccgcgccgtt
     3481 agggcccacc agcccataga tggagccctt ctccacctcc atggtgagcc catccagggc
     3541 gcggaagccg tcgaaggtct tgacgacgtt gtttaccttg atcattggac ctctccccct
     3601 ttttcccgga tgcgggcgat cagccgatcc cgcgtgaccc ccaggtacag cagctcggct
     3661 gtcagactgt cgaactgccc cagcagccgg tcccgccgct cctcatccac cccggtgcgg
     3721 ggggccgcga agctgccctt cccagggata gaatagacat agccctccgc ctccagggac
     3781 tcataggccc gctggatggt gttggggttg atggcaagat tgctggccag ggaccgcaca
     3841 gaggggagct tttccccctc ctggatggcg ccgctcacca tcagccggcg cagattgtcc
     3901 cgcacctgct cgtagatggg ccggg
//
LOCUS       DBMDSJ010000117         3894 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6039_length_3894_cov_1.488795, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000117 DBMDSJ010000000
VERSION     DBMDSJ010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6039_length_3894_cov_1.488795"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="ACZPAQ_03980"
     CDS             <1..476
                     /locus_tag="ACZPAQ_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HIR0903174.1"
                     /translation="PEYPTINTIVAHQDALSLGSQALINMQLEGMSIGLEPSPENLHG
                     LTHRELGDYTDTLALLMETSNPSQGRLRGKTDENLVVTGQDKYYLKAAGYGRLYDFGY
                     DADGAPISERVARHVTGVAQFAVAFNELGLGNLVINGIPSYVDMAADIGQFLAPD"
     gene            502..1452
                     /locus_tag="ACZPAQ_03985"
     CDS             502..1452
                     /locus_tag="ACZPAQ_03985"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR0903175.1"
                     /translation="MKKQMLSLFLALALTGCAAPGPGSTSAPAESGAAPAGSEAPASA
                     SQEESGYDRREAIERPEIGAEVVIDPPYDFDPAEMVPGDFGDYFTDCDYPTQVSTYKL
                     AEGTDIENEVTVLQGEEEGPTIYIVAGVHGDEIAGWMTGNLVKKIGIRAGTVHILSPA
                     NRWGAEADPRVRYVTEQEDLNRSFPGDPEGNMAQRVADSIYQDIQRVDPVFLFDLHEA
                     RANAENKDFLGSSLIYTDLSEMSDMYLEMLMATESGELCSERFNFYGPGPIGSINNTV
                     TTQLGIPTITVETYRGYELERRIGDQLDIVEYVLRYYGLI"
     gene            1574..3004
                     /locus_tag="ACZPAQ_03990"
     CDS             1574..3004
                     /locus_tag="ACZPAQ_03990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903176.1"
                     /translation="MDLKREHELGQTAEARKRTDRDLRCIGWGLVLLGVLMPALVTVE
                     NTGIYRTLMNGIYQGQEIYVLLAALKLVLLNAVRAYPHYMGVFFLVETFRGMELRRRL
                     LASIAAVGILIPGVYLLINRIYGIRYDFGIPALSMIAMIWVLAKIDFSFVGLMKKGLM
                     VAFFITALQFLDMMPLLWNFPFGRGESSYDIKLVSKFLQADSFLQAMATIFFALFLFM
                     SVLLLLLVVDENNIRRISEQKELNEHMLMETRMRILENRTYMELRHLVHDLKSPLTSM
                     QALVGVVKLSAGRRGDQTEGEYLERIETMIDRMSSMISEILHEEQRSVVTTQELLDAV
                     MAQISSAEYADLVHTENRAPEAKIRINKIRFSRVLINLVENAAYAVRRPDGKIWIRVE
                     ETEGAEIRFQVEDNGTGIPRELLGEIWEKGFSIRSSSGLGLNFVQQVVEQCGGSVELE
                     SVEREGTVVTVSLPPCEEDVCETGPV"
     gene            2985..3554
                     /locus_tag="ACZPAQ_03995"
     CDS             2985..3554
                     /locus_tag="ACZPAQ_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903177.1"
                     /translation="MRQDQYELKGAVMEAPLEWQGLRLVPGRGGVLVTGLEGTDQRRF
                     QRQLTVLKGYAALAADRRGERPLRLRQEDLPEREGPETLAAVWYGRAAEAEDQHGTLV
                     ACYNGFGALPGGFAAVEESLARWAGRPLGEKLLDYVQAVRREEGDLAEKAEPFQTLPS
                     WTDRETGELLRALLRALCEGSGGRGSAQG"
     gene            3567..>3894
                     /locus_tag="ACZPAQ_04000"
     CDS             3567..>3894
                     /locus_tag="ACZPAQ_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903178.1"
                     /translation="MEKRAKQLEYLITSMILLGIGMLFFFMNESVNTYTVLRFGLFQI
                     MQSVLLLICAILLWYRAYVVRREQETAEGGAETLGAAPAAPEEAGEEKREAKRDAVDS
                     VNATMLL"
BASE COUNT          756 a         1101 c         1379 g          658 t
ORIGIN      
        1 ctcccgagta ccccaccatc aacaccatcg tggcccatca ggatgccctg tcactgggct
       61 cccaggcgct gatcaacatg cagctggagg gaatgagcat cggcctggag ccctctcccg
      121 agaacctgca cggcctgacc caccgggagc tgggcgacta caccgatacc ctggccctgc
      181 tgatggagac ctccaacccc tcccagggcc gcctgagagg aaagaccgac gaaaacctgg
      241 tggtcaccgg acaggataag tactatctga aggcggctgg ctacggccgt ctgtatgact
      301 tcggctatga cgcggacggc gcccccatct ctgagcgggt agcccgtcat gtcaccggcg
      361 tggctcagtt cgcggtggcc ttcaacgagc tgggattggg caacctggtc atcaacggca
      421 tcccgtccta tgtcgatatg gcggcggaca tcgggcagtt cctcgctccc gactgagcgc
      481 ggaaagttgg gaggtgaagg catgaaaaag cagatgttga gtctcttcct ggccctggcc
      541 ctcaccggct gcgccgcccc agggccgggg agcacctccg ccccggcgga gagcggagcg
      601 gcccccgcag gcagcgaggc gccggcctcc gcctcccagg aggagagcgg ctatgaccgc
      661 cgggaggcca tcgaacggcc ggagatcggt gcggaggtgg tcatcgaccc gccctatgat
      721 ttcgaccccg cggagatggt gcctggcgac ttcggggact acttcacaga ctgcgattat
      781 cccacccagg tgagcaccta caagctcgcc gaggggaccg acatcgaaaa tgaggtgacg
      841 gtgctccagg gggaggagga gggacccacc atctacatcg tggcgggagt tcacggggat
      901 gagattgccg gctggatgac cgggaacctg gtcaagaaga tcggcatccg ggccgggacg
      961 gtccatatcc tgtccccagc caaccgctgg ggcgcggagg cggaccccag agtccgctat
     1021 gtgacggagc aggaggatct gaaccggtcc ttcccggggg acccggaggg gaatatggcc
     1081 cagcgggtgg ccgactccat ctaccaggac atccagaggg tggacccggt gttcctcttt
     1141 gacctgcacg aggcccgggc caacgcggag aacaaagatt tcctgggcag ttcgctgatc
     1201 tacaccgacc tgagcgaaat gagcgacatg tacctggaga tgctgatggc cacggagtcc
     1261 ggcgagctgt gctccgagcg attcaacttc tatggacccg gccccatcgg gagcatcaac
     1321 aatacggtga ccacccagct gggtatcccc accatcacag tggagaccta ccggggctat
     1381 gagctggagc gccggatcgg agaccagctg gacatcgtgg agtacgtgct gcggtactac
     1441 ggcctgattt gagtgtgcag cggcctgaag ccggactcgg gatacaaaag aaaagacacg
     1501 gcaagccttg ccccgggcat ccagggcaag gcttgcggtg tattccttgg gcggaccgca
     1561 ggggaagagg gggatggact tgaaacgaga gcatgagctg ggacagacgg cagaggccag
     1621 aaaaagaacg gaccgggacc tgcggtgcat cggctggggc ctggtgcttc tgggggtgct
     1681 gatgcccgcc ctggtgacgg tggagaacac cggcatctac cgcacgctga tgaacggcat
     1741 ctatcagggg caggagatct acgtgctgct ggcggcgctg aagctggtgt tgctcaatgc
     1801 ggtgcgggcc tatccccact acatgggcgt gttttttctg gtggagacct tccggggcat
     1861 ggagctgaga cggcggctgc tggcctccat cgctgcggtg ggcatcctga tccccggagt
     1921 gtatctcctc atcaaccgga tctatggcat ccgctatgac ttcgggatcc cggcactgtc
     1981 catgatcgcc atgatctggg tgctggccaa gatcgatttc agctttgtgg ggctgatgaa
     2041 gaaggggctc atggtggcct ttttcatcac ggcgctgcaa ttcctggaca tgatgcctct
     2101 gctgtggaat tttcccttcg gcagaggaga gagttcctat gacatcaagc tcgtctccaa
     2161 gttccttcag gcggacagct ttctccaggc catggccacc atcttttttg ccctgttcct
     2221 cttcatgtct gtgctgctcc tgctgctggt ggtggatgaa aacaacatcc gccgcatcag
     2281 cgagcagaag gagctcaacg agcacatgct gatggagacc cggatgcgca tcctggagaa
     2341 ccggacctat atggaactgc gccacctggt ccatgatctg aagtcccctc taaccagcat
     2401 gcaggccctg gtgggggtgg tcaaactgtc cgccggcagg agaggggacc agacggaagg
     2461 ggagtatctg gagcggatcg agactatgat agaccggatg agcagcatga tttccgagat
     2521 cctgcacgag gagcagcgga gcgtggtgac cacccaggag ctgctggacg ccgtgatggc
     2581 ccagatatcc agcgcggagt atgcggacct ggtccacacg gagaaccggg ctccagaggc
     2641 gaagatccgg atcaacaaga tccgattctc ccgggtgctc atcaacctgg tggagaacgc
     2701 cgcctatgcg gtccggaggc cggacggcaa gatctggatc agggtcgagg agacggaggg
     2761 ggcagagatc cgctttcagg tggaggacaa cgggacaggg attcccaggg agctgctggg
     2821 cgagatctgg gagaagggct tctccatccg cagctccagc ggactgggac tgaatttcgt
     2881 acagcaagtg gtggagcagt gcggcggcag tgtcgaactg gagagcgtgg agcgggaggg
     2941 aacggtggtc accgtctccc tgcccccctg tgaggaggat gtgtgtgaga caggaccagt
     3001 atgagctgaa gggagcggtg atggaggcac cgctggagtg gcagggcctc cgtctggttc
     3061 ccggacgggg gggcgtcctg gttacggggc tggaggggac ggaccagcgg cggttccagc
     3121 ggcagctgac cgtactgaaa gggtacgccg ccctggcggc ggacaggcgg ggggaacgcc
     3181 ccctccgcct gcggcaggag gacctgccgg agcgggaagg gccggagacc ctggccgcag
     3241 tctggtacgg ccgggcggcg gaggcggagg accaacacgg caccctggtg gcctgctaca
     3301 acgggttcgg cgccctgccg gggggctttg ccgctgtgga ggagagcctg gcacgctggg
     3361 ctgggcggcc cctgggggag aagctgctgg actatgtcca ggcggtccgg cgggaggagg
     3421 gggacctggc cgagaaggcg gagcccttcc agaccctgcc cagctggacg gaccgggaga
     3481 cgggggagct gctgcgcgcg ctgctgcggg cgctgtgtga gggatctggc gggcgcggga
     3541 gcgcccaggg ataagggggg agcggagtgg agaagcgagc caagcagctg gaatacctga
     3601 tcaccagcat gatcctgctg ggcatcggga tgctgttttt cttcatgaac gagagcgtca
     3661 atacctatac ggtgctgcgt tttggcctgt ttcagatcat gcagagcgtg ctgctgctga
     3721 tctgcgctat cctgctctgg tatcgggcat atgtggtccg gcgggagcag gagacggccg
     3781 aagggggggc ggagacgctg ggagcagccc cggctgcgcc ggaggaggcc ggagaggaaa
     3841 agcgggaggc caagcgggac gcggtggaca gcgtcaatgc caccatgctg ctgg
//
LOCUS       DBMDSJ010000118         3890 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6047_length_3890_cov_1.558459, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000118 DBMDSJ010000000
VERSION     DBMDSJ010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6047_length_3890_cov_1.558459"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(61..666)
                     /locus_tag="ACZPAQ_04005"
     CDS             complement(61..666)
                     /locus_tag="ACZPAQ_04005"
                     /inference="COORDINATES: protein motif:HMM:NF022636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIR0903179.1"
                     /translation="MEHNVVVRMAKVLYGMVLAVFVFNLLVLPLVPGYTMMAYEGMGM
                     GHPSISSLMGTMRSLLGAGVPAWEILVVRPLAMLGGDWSGYGPEVWWSAAFFLGCGIC
                     TAVLLWQARCILSTIIVQTPFQRSNARSMKRAAASCWGIALLAAVRMAVWFLRLRSAA
                     PLFTFTALFIPVSLMAGLLFLVMSALFRQAAELKEDQDLTI"
     gene            complement(972..1535)
                     /locus_tag="ACZPAQ_04010"
     CDS             complement(972..1535)
                     /locus_tag="ACZPAQ_04010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR0903180.1"
                     /translation="MEQLRITDTPAGPISYLLTRKRVKNLNLRVRNGQVSLSIPLGCP
                     AEEADAMVRAKCRWIIQALERQWAPGPELPPEPARRECLRLLGEALDRVYPLVQPLGV
                     AWPELRLRRMKSQWGNCHWRQGYITLNTALARCPETLRDYVALHELVHFVHPDHGPGF
                     YGLMDRLMPDWRQRRKELRRYGGALAG"
     gene            complement(1902..2540)
                     /locus_tag="ACZPAQ_04015"
     CDS             complement(1902..2540)
                     /locus_tag="ACZPAQ_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903181.1"
                     /translation="MKKLKISSLMNEYQDQESEPVPQPGLVDGERVRQRVLGQVAGRR
                     RRKLRRAVIAAVAAAACLGLVAWTWGERIYQMASGGQVTIGEGYGSVTMSDGKGEEGE
                     PLVVSLEAGRLWFVAGGERTDITDLVDEDTPYVHTYTDGEGVLHYFLVGGTPEDYGWF
                     EGLKAPDGSGGGAGVLSSVVPEPGTVETAEEPGWYTAGTAQVQALWRAQQGK"
     gene            complement(2485..3081)
                     /locus_tag="ACZPAQ_04020"
     CDS             complement(2485..3081)
                     /locus_tag="ACZPAQ_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903182.1"
                     /translation="MTPIQEQEMWRALRRGDPRGLEELMSRYTPYVAAVAARVLPGRP
                     QDWEELTADVFLSAWEHRKALRPEQIRGWLGKVARNRALNRLRASREVLALEEDVLCL
                     AEDGPQRLLEEREQAELVRRALGELERPDRELFVRHYYYGQTVARAAEEMGLNPSTAK
                     TRLRRGREKLKEHLRKAGFCVEEAEDLKPDERVSGSGE"
     gene            complement(3169..>3890)
                     /locus_tag="ACZPAQ_04025"
     CDS             complement(3169..>3890)
                     /locus_tag="ACZPAQ_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HIR0903183.1"
                     /translation="SPSCCARAGRGYGLLFQHEGEGRTLHAHMDRFRDGQALDAQILL
                     TQEPEESIVMCTPFEKPGQFYFNQKINCMRAQGKVTLGDREYVFDPEDSFAVLDWGRG
                     VWTCRSTWYWGSASGMVDGVPFGFNIGYGFGDTSAATENALFYGGKLHKLSQVTFHIP
                     RKNGQEHYLAPWTFDSDDRRFRMKFRPILDRAACPSAGVVRSGQHQVFGSFTGTAVLD
                     DGTAVRLKELLGFAEKVDDRW"
BASE COUNT          647 a         1440 c         1136 g          667 t
ORIGIN      
        1 acatcttgcg ccgggccatc atcacgtcca ggttgactac gatcttcatg ctccgccccc
       61 tcagatggtc aggtcctggt cctctttcag ctccgccgcc tggcggaaca gggcagacat
      121 caccaggaaa agcaggcccg ccatcagcga caccggaatg aacaaggctg tgaaggtgaa
      181 cagcggggct gcgctcctca gccgcaggaa ccacaccgcc atccgcactg ccgccagcag
      241 ggcgatgccc cagcagctgg ccgccgcccg cttcatggac cgggcattgg accgctgaaa
      301 gggagtctgg acgatgatcg tggacaggat gcaccgggcc tgccacagga gcacggcggt
      361 acagatgccg cagcccagga aaaaggccgc gctccaccac acctccggcc cgtaccccga
      421 ccagtcgcct cccagcatgg ccaggggccg caccaccagg atctcccatg cgggcacacc
      481 agcccccagc agggagcgca tggtccccat cagggagctg atgctggggt gtcccatccc
      541 catcccctca taggccatca tggtatagcc cggcaccaga ggcaggacca gcagattgaa
      601 gacaaatacc gccagcacca tcccatacag gaccttggcc atgcggacga ccacgttgtg
      661 ttccatgagc gcggctccct cctttgtaat tgcccatatc ctaccacctc aaaacacgat
      721 tgtcaatata tttttatcgt ttttcgataa atccaaaagg aaaagaaacc tccggacggg
      781 ggagtccgga gacgcatccc ttcaaaaacc gctctctctt tctctcacca gcgcacgcgg
      841 cgccacactg actgttccta ttacagagat aactgtttct tttccattac tatctctgtt
      901 actgttacgg ggccctattt gcacgggatc gtacaaccag agccccccgc cgcgccgggg
      961 tgagggggcc gtcatcccgc cagtgccccg ccatagcgcc gcagctcctt ccgccgctgc
     1021 cgccagtccg gcatcagccg gtccatcagc ccgtaaaagc ccggcccgtg gtccgggtgg
     1081 acaaagtgga ccagctcatg gagggccaca tagtcccgca gggtctccgg gcagcgggcc
     1141 agggcggtgt tcagggtgat gtagccctgc cgccagtggc agttgcccca ctggctcttc
     1201 atacggcgca gcctcagctc cggccaggcc accccaaggg gctgcaccag aggatagacc
     1261 cggtccagcg cctcccccag cagccgcagg cactcccgcc gggcgggctc cggcggcagc
     1321 tccggccccg gggcccactg ccgctccagc gcctggatga tccaccggca tttggcccgg
     1381 accatggcgt ccgcctcctc tgccgggcag cccagcggga ttgacaggct cacctgaccg
     1441 ttccgcaccc ggaggttgag gtttttcacc cgctttcggg tgagcagata ggagatgggc
     1501 cccgccggag tgtccgtgat ccgcagctgc tccatcctgt tccgccccct ctctgtaccc
     1561 ggtctttcca tggaggataa cagaattttg gagaaaatgc aagggcggtg agtgcctcag
     1621 ctccgcccgg tgtgctcccc ccataccgct ctcccggcgg ccggttcggg tcgtacaacg
     1681 gcgcccctct cctcagcccc tgtgcagggc ggcatatgct gtgccggatg aggagggcaa
     1741 tcgccccaga aggccgggcg ggtctgggcc tcgcccctgc acaggggccg ggaggggtgc
     1801 gcaccataca gggcccgaag ccacggcaca cggcccgtgg gccggcattt ctccccgaac
     1861 aaaggccgcg ggcggcctga cagccgcccg cttctcccat tttattttcc ctgctgggcc
     1921 cgccagaggg cctggacctg cgccgttccc gccgtgtacc atccgggctc ctccgccgtc
     1981 tccacggttc ccggctccgg caccacgctg ctcagcacac cggcgccgcc gccgctgccg
     2041 tccggggctt tcagcccttc aaaccagccg tagtcctccg gggtgccccc caccaggaaa
     2101 tagtggagca ccccctcccc gtcggtgtag gtgtggacgt agggggtgtc ctcgtccacc
     2161 agatcggtga tatcggtccg ctcgccgccg gccacgaacc acagccgtcc cgcttccaga
     2221 gagaccacca gcggctcccc ctcttctccc ttcccgtccg acatggtgac ggacccatag
     2281 ccctctccga tggtcacctg gcctccggag gccatctgat agatcctctc tccccaggtc
     2341 caggccacca ggcccaggca ggccgccgcg gccacggccg cgatcacggc ccggcggagc
     2401 ttccgtcttc tccgtccggc cacctgtccc agcacccgct ggcgcacccg ctccccatcc
     2461 accagtcccg gctgggggac aggctcactc tcctgatcct gatactcgtt catcaggctt
     2521 gagatcttca gcttcttcaa cacaaaaccc cgctttccgc aaatgttcct tcagcttctc
     2581 ccggccccgg cggagccggg tcttggcggt ggacgggttg agccccatct cctccgccgc
     2641 ccgggccacc gtctggccgt agtagtagtg ccggacgaag agctcccggt ccggcctctc
     2701 cagctctccc agggcccgcc ggaccagctc cgcctgctcc cgctcctcca gcaggcgctg
     2761 ggggccgtcc tccgccagac agagcacgtc ctcctccagc gccaggacct cccggctggc
     2821 ccgcaggcgg ttcagcgccc ggttccgggc cacctttccc agccagcccc ggatctgctc
     2881 cgggcgcagc gccttccggt gctcccaggc cgacaggaac acgtccgctg tcagctcctc
     2941 ccagtcctgg ggccggccgg gcagcacccg ggcggccacc gcggccacat agggggtata
     3001 gcggctcatc agctcctcca gcccccgggg gtccccccgg cgcagggccc gccacatctc
     3061 ctgttcctga atgggtgtca catcctttcc ctgaaacgcg tttcacccct ataaacacag
     3121 gagacgggct tttggtttca atttgcggcg tatttgtcgg gatcttgcct accagcggtc
     3181 gtccaccttc tccgcgaagc ccagaagctc cttcagccgg accgccgtgc cgtcgtccag
     3241 caccgcggtg ccggtgaagc tgccgaacac ctggtgctgg ccggaccgca ccacgccggc
     3301 actggggcag gccgcccggt ccaggatggg gcggaacttc atccgaaagc gccggtcgtc
     3361 gctgtcgaag gtccaggggg ccaggtagtg ctcctggccg ttcttccgtg ggatatggaa
     3421 ggtcacctgg gagagcttgt ggagtttccc gccataaaac agggcgttct ccgtggcggc
     3481 ggaggtgtct ccgaagccgt atccgatgtt gaagccgaat gggaccccgt ccaccatgcc
     3541 ggaggcggag ccccagtacc aggtgctgcg gcaggtccac acccctctgc cccagtccag
     3601 caccgcgaag gagtcctccg ggtcaaagac atactcccgg tcccccagag tgacctttcc
     3661 ctgggcccgc atgcagttga tcttctggtt gaaatagaac tgccccggct tttcaaaggg
     3721 cgtgcacatc acgatggact cctccggctc ctgggtgagc aggatctggg cgtccagcgc
     3781 ctgcccgtcc cggaagcggt ccatatgggc gtggagggtg cgcccctctc cctcatgctg
     3841 gaacagcagg ccgtagcccc gcccggccct ggcgcagcat gagggagaga
//
LOCUS       DBMDSJ010000119         3880 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6059_length_3880_cov_1.684043, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000119 DBMDSJ010000000
VERSION     DBMDSJ010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6059_length_3880_cov_1.684043"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="ACZPAQ_04030"
     CDS             <1..193
                     /locus_tag="ACZPAQ_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903184.1"
                     /translation="RAKGGRESLTLHVYRENSPAVRFYQREGFAIRAAEREENTGREE
                     YHMEWHRGGQHLPPRRGEP"
     gene            302..982
                     /locus_tag="ACZPAQ_04035"
     CDS             302..982
                     /locus_tag="ACZPAQ_04035"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0903185.1"
                     /translation="MAEELFGGQDVGATPSYKEQAYRLIKEAILFNRFRVGAIYSQDD
                     ICKELGISRTPVREALLELQKDGYVSFSRGKGVQVVPVSDRDARDILETRMYIECINA
                     KLAANRAAQRDLQAMRESLDRLRDQLSTRDGQHLYRLDHIFHRAVAAATQNRLLYREC
                     ELLLDHYLRFEVKSVYNNSIDAKIVFDEHVAICDAIAEGSAERAEQAMARHLTNSYRR
                     TVSRFWAE"
     gene            1356..2741
                     /locus_tag="ACZPAQ_04040"
     CDS             1356..2741
                     /locus_tag="ACZPAQ_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR0903186.1"
                     /translation="MLIATWIVTFVIIVGIGVWSGTKIKSANQWSGGDKTMGPLTLGC
                     VFAAWQIGGMAVVGAAQNGYTLGISGAWYSIAGSFYFIALALFAKLIRNNMPGDSVPI
                     YLQKRFDTKTSRLYSYAWIVYGFLYIPYQLKTVASIIQIAIPGLNITLAMLIGLTIAV
                     IYTSFSGMRGMSAVGRIVCFGIYILLIAFVLMTLPKFGGFTVMLSELPAEYSSMGNMP
                     TQRIIAWILGGCLSTAVMQSVLQPLLAAKSASAARTGSILGYVLAAPICLLTATCGIF
                     AKASGADLGDGATAFAYSIKTFSNPAFAGIIFAFATMIIAATMATMMLATGTIITNVY
                     KNDINPNVDDAKLLKLSKIITFVFAYLTLIPAVIMPSAALTNLFLTLQHVAAAPVSFA
                     ILVGLTWKRCTRQGAFWAMASGMVVGVAWMLLGLTDTLEAVYPTVLVSYTVGVVVSLA
                     TSKKTHGLAKE"
     gene            2807..>3880
                     /locus_tag="ACZPAQ_04045"
     CDS             2807..>3880
                     /locus_tag="ACZPAQ_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR0903187.1"
                     /translation="MSEAKKQQYMWPSRWSKPSAHGDCGAVSCNNIYATRAGLEMLRA
                     GGNAFDAAVAVSLTLSVVEPHHSGIGGGCFSLLYSQRDQKVLALDARGVAPAKAGEDL
                     FLKDGEVQDEWKDLGGQSVAIPGLLRAMETMLREYGTMTLAQAAAPAIRCAREGFGTS
                     YTGELTMEDDSVRRKLDLSPQFRRLYLKPDGSRYRFGEKQVNSELAGLLELVAAEGAD
                     AFYTGPVARKMVEQINQRGGCFTLEDLSRYQPKYRTPVKTTYRGLEVAAFAPPSGGCA
                     VVEMLNILEHSDLAAMGHNTAASIHAIAEAMKLGFADRSVALGDPDFVQVDVERLTSK
                     AHAAERYALSSPETAGEYAPVMRG"
BASE COUNT          761 a         1171 c         1227 g          721 t
ORIGIN      
        1 gcgggccaag ggggggcggg agagcctgac actccacgtg taccgggaga acagcccggc
       61 ggtccgcttt taccagcggg agggctttgc catccgggcc gcggagcggg aggagaacac
      121 cggccgggaa gagtaccata tggagtggca caggggagga cagcaccttc caccgcggcg
      181 cggggagcct tgatccgcta agaccccgct gccccgggag caggtccgca gctccgcttg
      241 acaccgggcg tccggggatg gtaagatggg gaaaagctgg catgggacgg gaggagagct
      301 tatggcagag gagctgtttg gcggacagga tgtgggggcc acgccctctt ataaagaaca
      361 ggcataccgc ctgatcaagg aggcgatcct cttcaaccgg tttcgggtag gggccatcta
      421 ctcccaggac gacatctgca aggagctggg catcagccga accccggtgc gggaggctct
      481 gctggagctc cagaaggacg gctatgtctc cttctcccgg ggcaaggggg tgcaggtggt
      541 gccggtgtct gaccgggacg cccgggacat cctggagacc cggatgtata tcgagtgcat
      601 caacgcgaag ctggcggcga accgtgctgc ccagcgggac cttcaggcca tgcgggagag
      661 cctggaccgc ctccgggacc agctctccac cagggatggg cagcacctgt accgcctgga
      721 ccacatcttc caccgggcag tggcggcggc cactcagaac cgcctgctct accgggagtg
      781 cgagctgctc ctggaccact atctccggtt tgaggtcaag tcggtgtata acaactccat
      841 cgacgccaag atcgtctttg atgagcatgt ggccatctgc gatgccatcg cggaagggag
      901 cgccgagcgg gcagagcagg ccatggcccg acacctgacc aactcctacc gccgcaccgt
      961 cagccgtttc tgggcggaat agtggaagaa aacatggacg gccgtgtccc gggaggatgc
     1021 ggccgtttga cttgtctgaa tatgtaaata tatcccatat aaaatgtttt caaccagagc
     1081 tcgaaaaagt tcttgacatt gcccctaaaa tatcttatga taaggccata aaacaagtgt
     1141 attggagagc gaacagaata gcggttctgc gcgctctcct tttttagagc tcaagtaata
     1201 tattacatat ttaataaaat aggaaaaatg taatatgttg caaagggagg gcacaggcag
     1261 gtttgagcag ctgcggtggg cggtatctgt tccgcatccg gaggcggggc ggcaccggca
     1321 tccgctgcaa ataaacaaaa gggagagaga accgtatgtt aatcgcaaca tggatcgtga
     1381 cctttgtcat tatcgtgggg atcggtgtct ggtccggcac caagatcaaa agtgccaacc
     1441 agtggtcggg cggtgacaag accatgggcc ccctgactct gggctgtgtc tttgccgcct
     1501 ggcagattgg cggcatggcc gtggtagggg cggctcagaa cggctacacc ctgggcatct
     1561 ccggggcctg gtactccatc gccggctcct tctacttcat cgcgctggcc ctgttcgcca
     1621 agctgatccg caacaacatg cccggcgact cggtgcccat ctatctgcaa aagcgcttcg
     1681 acaccaagac cagccgcctg tactcctacg cctggatcgt ctacggcttc ctgtacatcc
     1741 cctatcagct caagaccgtg gcttccatca tccagatcgc catccccggt ctgaacatca
     1801 cgctggccat gctcatcggc ctgaccatcg ccgtgatcta caccagcttc tccggcatgc
     1861 ggggcatgtc cgctgtgggg cgcatcgtat gcttcggcat ctacatcctg ctcattgcct
     1921 tcgtgctcat gacgctgccc aagttcggcg gcttcaccgt gatgctctct gaacttcccg
     1981 cagagtactc ctccatgggc aatatgccca cccagcgcat catcgcctgg atcctgggcg
     2041 gctgcctgtc caccgcagtg atgcagtccg tgctccagcc cctgctggcc gcaaagagcg
     2101 cttccgccgc ccgcaccggc tctatcctgg gctatgtgct ggctgccccc atctgcctgc
     2161 tcaccgccac ctgcggcatc tttgccaagg cgtccggtgc ggacctgggc gacggtgcca
     2221 ccgcatttgc ctactccatc aagaccttca gcaatccggc tttcgctggg atcatttttg
     2281 cctttgccac catgatcatc gcggctacca tggccaccat gatgctggcc acaggcacca
     2341 tcattaccaa tgtctataaa aacgacatca atcccaacgt ggacgacgcc aaactgctga
     2401 agctgtccaa gatcatcacc ttcgtctttg cctacctgac tctgatccct gcggtcatca
     2461 tgcccagcgc cgccctcacc aacctgttcc tcaccctgca gcacgtggcc gccgccccgg
     2521 tgagcttcgc catcctggtg ggcctgacct ggaagcgctg cacccggcag ggggccttct
     2581 gggccatggc cagcggcatg gtggtaggtg tggcctggat gctgctgggc ctgaccgaca
     2641 cactggaggc ggtctacccc acagtgctgg tcagctatac agtgggtgtt gtggtatctc
     2701 tggcgaccag taaaaagacg catggtttgg cgaaggaata atggaatccg aacaagactc
     2761 gatgaaaaca ctgtccggcg cggccgggaa gggaagcgga tcaagtatga gtgaagcaaa
     2821 gaagcagcag tacatgtggc cctcgcggtg gagcaagccc tccgcccacg gggactgcgg
     2881 agcggtcagc tgcaacaata tctatgccac ccgggcgggg ttggagatgc tgcgggccgg
     2941 agggaacgcc tttgacgccg ccgtggcggt ctctctgacc ctctctgtgg tggagcccca
     3001 ccactccggc atcgggggcg gctgtttcag cctgctgtac tcccagcggg atcagaaggt
     3061 cttggcgctg gacgcccggg gcgtggctcc cgccaaggcg ggggaggacc tgttcctcaa
     3121 ggatggagag gtccaggacg agtggaagga cctggggggc cagtcggtag ccattccggg
     3181 gctgctgaga gccatggaaa ccatgctgcg ggagtacggc actatgacgc tggcgcaggc
     3241 ggcggctccc gccatccgct gtgcccggga gggcttcggc accagttata ccggagagct
     3301 caccatggag gacgactcgg tccggcggaa gctggatctt tcaccccagt tccggcggct
     3361 gtatctgaag ccggacggga gccgctaccg cttcggtgag aagcaggtga actctgagct
     3421 ggccggcctg ctggagctgg tggcggcgga gggggcggac gccttttaca ccgggccggt
     3481 ggcccggaaa atggtggagc agatcaacca gcggggcggg tgctttaccc tggaggacct
     3541 gagccgatat cagcccaagt accgtacccc ggtgaagacc acctaccggg ggctggaggt
     3601 ggcggccttc gcgccgccca gcggcggatg cgccgtggtg gagatgctga acattctgga
     3661 gcacagcgat ctggccgcca tgggacacaa caccgccgcc tccatccatg ccatcgcgga
     3721 ggccatgaag ctgggctttg cggaccgaag cgtggctctg ggcgatcccg acttcgtcca
     3781 ggtggacgtg gagcggctga ccagcaaggc ccacgcggcg gagcggtatg ccctctcatc
     3841 accggagacg gcgggggaat acgctccggt gatgagaggg
//
LOCUS       DBMDSJ010000120         3875 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6067_length_3875_cov_1.739269, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000120 DBMDSJ010000000
VERSION     DBMDSJ010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6067_length_3875_cov_1.739269"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="ACZPAQ_04050"
     CDS             <1..463
                     /locus_tag="ACZPAQ_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR0903188.1"
                     /translation="LTPFLVAALVLVLTQLINHTKVGMAMRAVAKDFETSQLMGIKIN
                     SVISVTFVIGSALAAVGSMLYFTTYPAIVPTAGAMPGLKAFVAAVFGGIGSIPGAVIG
                     AFLIGICENFVKIMPFDGATTFVDAFTFALLIIILVFKPTGLFGEKATDKV"
     gene            479..1849
                     /locus_tag="ACZPAQ_04055"
     CDS             479..1849
                     /locus_tag="ACZPAQ_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR0903189.1"
                     /translation="MIQDRKSKKGAAAGLIFAAVVLVLVVLLENLSSLLPSVPAVLQP
                     TSQLFTVLKKGAVYSLVAVSMNLLNGFTGLFSLGQAGFMLLGAYTYAILTVPVSAKDS
                     VYYLYGGSAVPFSLPDLFGGVDTPAGLILGVLLAILLGGCVAALFAWLIGLPVLRLKS
                     DYLAIATLGFAEIIRAVFQWQALGPVTNGANMITQIPSFNNFNLTDGSGKVFLRLSTF
                     FPFLIAILCIIVIVMLINSSYGRAFKAIREDEIAAEAMGINLAKHKMLSFCISSFFAG
                     VGGALFAMFVNNAQAKVYTSTMTYEILLIVVIGGIGSISGSCLATFLYVACAEWWLRF
                     LDSPMTFTSVTDTRRILFILVFAALVIGGALLLIRRERRTTKKVWKELLVSLAALALL
                     VWLAFFIISGSLTVPLLRTGFRMVVFSVIIMIVVLFFRRGIMGDKEFSDILFARRNAS
                     GKGAVK"
     gene            1846..2700
                     /locus_tag="ACZPAQ_04060"
     CDS             1846..2700
                     /locus_tag="ACZPAQ_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903190.1"
                     /translation="MSQVENVLRLEHVTMQFGGVVAVNDLSLEVNQGEIVALIGPNGA
                     GKTTAFNCITGVYEPTNGRISFLGEPIVENHPQGRMQKLYQGENQGMFSQVLSPTPDR
                     ITQKGIARTFQNIRLFSALTVFENVLIAKHMRARQNVFSAAFRLNRAEEKRMREEAMS
                     LLEEQNLAHLKDEVAGSLPYGLQRHLEIARALATAPKLLLLDEPAAGMNPQETQELTD
                     FIHKIRDEYDLSILMIEHHMDLVMRISDRIYVLDFGKQIAQGTPAEVQNDPRVIEAYL
                     GVADDAED"
     gene            2687..3385
                     /locus_tag="ACZPAQ_04065"
     CDS             2687..3385
                     /locus_tag="ACZPAQ_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903191.1"
                     /translation="MLKIDELKVSYGGIEAVKGITFEVPERQIVTLIGANGAGKSTTL
                     RTIAGLVKPAHGRIHLQGEDITGLSPDRIVSKGVTLVPEGRRVFPDLSVVENLKIGAY
                     LRKDDLEDDLNWVYDLFPRLKERSWQAAGTLSGGEQQMLAVARALMSRPKIIMMDEPS
                     LGLAPLVVKGIFEIIQEINKQGVTVLLIEQNANMALKIADLGYVLETGRITLTGSGRD
                     LLKNEDVKKAYLGT"
     gene            complement(3477..>3875)
                     /locus_tag="ACZPAQ_04070"
     CDS             complement(3477..>3875)
                     /locus_tag="ACZPAQ_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903192.1"
                     /translation="GQPVVLSEARNGDMAFFLYQEGSSLRYTYYENEPGLDFGYHFRA
                     SGTGHEETGVYVVQHGETLLVVSLNRVEEVEGIEVTDPESGTVRQTYPVAPGEPFAVL
                     VTLGEGAEDLALRAVDRRGETVPFENYYVR"
BASE COUNT          686 a         1304 c         1112 g          773 t
ORIGIN      
        1 cctcaccccc ttcctggtgg ccgccctggt gctggtgctc acccagctca tcaaccatac
       61 caaggtgggc atggccatgc gggctgtggc caaggatttc gagaccagcc agctcatggg
      121 catcaagatc aactcggtga tctccgtcac cttcgtcatc ggctccgccc tggctgcggt
      181 gggctccatg ctctacttca ccacctaccc cgccattgtc cccactgcgg gcgccatgcc
      241 cggcctgaag gccttcgtgg ccgcggtgtt cggcggcatc ggctccattc caggggcggt
      301 gatcggggcc tttctcatcg gcatctgtga gaactttgtg aagatcatgc cctttgacgg
      361 cgccaccacc ttcgtggacg cctttacctt cgccctgctc atcatcatcc tggtcttcaa
      421 acccaccggt ctcttcgggg aaaaggccac cgacaaggtg taaggaggga atgacgacat
      481 gatccaagac agaaaaagca aaaagggcgc cgcggcgggg ctgatcttcg ccgctgtggt
      541 gctggtgctg gtggtcctgc tggagaacct gagcagcctg ctgcccagcg tccccgccgt
      601 ccttcagccc accagccagc tgttcactgt actgaaaaag ggggcggtct actccctggt
      661 ggccgtgtcc atgaacctgc tcaacggctt caccggcctg ttctcactgg ggcaggcggg
      721 cttcatgctg ctgggcgcct atacctacgc catcctcacc gtccccgtct ccgccaaaga
      781 ttcggtgtac tacctgtacg ggggctcagc ggtgcccttc tccctgcctg acctgttcgg
      841 cggggtggac acccccgcgg gcctgatcct gggggtgctg ctggccatcc tgctgggcgg
      901 atgcgtggcc gccctctttg cctggctcat cggcctgcct gtgctgcgtc tgaagagcga
      961 ctacctggcc atcgccaccc tgggctttgc agagatcatc cgagcggtgt tccagtggca
     1021 ggcccttggg cccgtcacca acggcgccaa tatgatcact cagatcccct ccttcaacaa
     1081 cttcaacctc accgacggga gcgggaaggt gttcctccgc ctgtccacct tcttcccctt
     1141 cctcatcgcg attctctgca ttatcgtcat cgtgatgctc atcaactcct cctacggccg
     1201 ggctttcaag gccatccggg aggacgagat cgccgccgaa gccatgggca tcaacctggc
     1261 caagcacaag atgctctcct tctgcatctc ttccttcttc gccggagtgg ggggcgccct
     1321 gtttgccatg ttcgtcaaca acgcccaggc caaggtgtac acctccacca tgacctatga
     1381 gatcctgctc atcgtggtca tcggcggcat cggctccatc tccggctcct gcctggccac
     1441 cttcctgtat gtggcctgcg ccgagtggtg gctgcgcttt ctggacagcc ccatgacctt
     1501 cacctccgtt accgacaccc ggcgcatcct cttcatcctg gtcttcgccg ccctggtcat
     1561 cggcggggcc ctcctgctga tccgccggga gcgccgcacc accaaaaagg tttggaagga
     1621 gctgctggtt tccctggcgg ccctggccct gctggtctgg ctggctttct tcatcatcag
     1681 cggctccctc accgttcccc tgctgcgcac cggcttccgt atggtggtct tctcggtcat
     1741 catcatgatc gtggtcctct tcttccgccg gggcataatg ggggacaagg aattctctga
     1801 cattctgttt gcccgccgca atgcgtccgg aaagggggcg gtgaaatgag ccaagtggaa
     1861 aatgtcctcc ggctggagca cgtcaccatg cagttcggcg gcgtggtggc ggtgaacgac
     1921 ctgtccctgg aggtgaacca aggggagatc gtggctctga tcggccccaa tggcgccggc
     1981 aagaccactg ccttcaactg catcaccgga gtgtatgagc ccaccaacgg gcggatctcc
     2041 tttctgggag aacccatagt ggaaaaccac ccccaaggca gaatgcagaa gctctatcag
     2101 ggggagaacc agggtatgtt ttcccaggtc ctctccccca ccccggaccg gatcacccag
     2161 aagggcatcg cccgtacctt ccagaacatc cgcctgttct cggccctgac ggtatttgaa
     2221 aacgtgctca tcgccaagca catgcgtgcc aggcagaacg tgttttcggc cgccttccgc
     2281 ctcaacaggg cggaggagaa gcggatgcgg gaggaggcca tgtccctgct ggaggagcag
     2341 aacctggccc acctgaagga cgaggtggcc ggctccctgc cctatggcct ccagcgccat
     2401 ctggagattg cccgggccct ggccaccgcc cccaagctcc tgctcctgga cgagccggcc
     2461 gccggcatga acccccagga gacccaggag ctcaccgatt tcatccacaa gatccgggat
     2521 gagtacgacc tgtccatcct gatgatcgag caccacatgg acctggtcat gcggatctcc
     2581 gaccgcattt acgtcctgga cttcggcaag cagatcgccc agggcacacc ggcggaggtc
     2641 cagaacgatc ctcgggtcat cgaggcatat ttgggggtgg cagacgatgc tgaagattga
     2701 tgaactgaag gtgagctatg gcggtatcga ggccgtgaag ggcatcacct ttgaagtgcc
     2761 cgagcggcag atcgtcaccc tgatcggcgc caacggagca ggcaagtcca ccaccctgcg
     2821 caccatcgcc gggctggtaa agcccgccca cggccgcatc catctccagg gggaggacat
     2881 caccggtctg tcccccgacc gcatcgtctc caagggggtc accctggtcc ccgagggacg
     2941 ccgggtgttt cccgacctgt cggtggtgga gaatctgaag atcggggcct atctgcggaa
     3001 ggacgacctg gaagacgacc tgaactgggt gtacgatctg tttccccggc tgaaggagcg
     3061 ctcctggcag gcggcgggca ccctgtccgg cggtgagcag cagatgctgg ccgtggcccg
     3121 ggccctcatg tcccgaccga agatcatcat gatggatgag ccctccctgg ggctggcccc
     3181 cctggtggtc aaggggatct ttgagatcat ccaggagatc aacaagcagg gggtcaccgt
     3241 gctgctcatc gagcagaacg ccaacatggc cctgaagatc gcggacctgg gctatgtgct
     3301 ggagaccggc cgcatcaccc ttaccggctc cggccgggat ctgctgaaaa acgaggacgt
     3361 gaagaaggcc tatctgggca cctgagaacc cagtctctat ccaaatcgta aagcaggggc
     3421 aggacaatac attttgtatt gtcctgcccc tgctttttct gcggtttcgt tcccgttcac
     3481 cgcacgtagt agttctcaaa tggaacggtc tcccctctgc ggtccactgc ccggagggcc
     3541 aagtcctccg ccccttctcc cagagtcacc agcaccgcga agggctcccc cggggccacc
     3601 ggatatgtct gccggaccgt gccgctctcc gggtcggtca cctcgatccc ctccacctcc
     3661 tccactcggt tcagcgaaac caccagcagt gtctccccgt gctgcaccac atacaccccg
     3721 gtctcctcat ggccggtgcc gctggcccga aagtgatagc cgaagtccag cccaggctcg
     3781 ttttcatagt aggtgtagcg caggctggag ccctcctgat agaggaaaaa ggccatgtcc
     3841 ccgttccgcg cctctgacag caccaccggc tgccc
//
LOCUS       DBMDSJ010000121         3850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6114_length_3850_cov_1.982929, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000121 DBMDSJ010000000
VERSION     DBMDSJ010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6114_length_3850_cov_1.982929"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2087)
                     /locus_tag="ACZPAQ_04075"
     CDS             complement(<1..2087)
                     /locus_tag="ACZPAQ_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR0903193.1"
                     /translation="MPKKKNPDETKPKVTNPNVMGLRAEVLEQPITETLEINYMPYAM
                     SVIVSRAIPEIDGFKPSHRKLLYTMYQMKLLGGARTKSANVVGQTMKLNPHGDAAIYD
                     TLVRLSRGYGALLHPLVDSKGNFGKVYSRDMAWAASRYTEVRLDPICAELFRDIDQDT
                     VDFVDNYDGKLKEPTLLPTTFPNVLVSANQGIAVGMASNLCGFNLGEVCDTTIAYLKD
                     PDHDLMSTLKAPDLPTGGELLYDASELASIYRTGRGSFKVRAKWRYLKADNIIEVYEI
                     PYSTTVEAIMDKVAELIKSGKIREIADMRDETDLSGLKLTIDLKRGTDPDKLMAKLFK
                     STPLMDSQSCNFNILIAGMPRVMGVREILEEWTAWRSDCVRRRTYFSLKKKQEKLHLL
                     KGLKRILLDIDRAIRIIRETEEDAEVVPNLMIGFGIDQIQAEFVADIRLRNINKEYIL
                     KRTQEVDSLEEEIADLQDLVNSPRRIRQVIIGELEQVKKKYALPRRTDIVYDHQTEEL
                     LTAEEEVPDYPVNLFLSREGYLKKITPQSLRMSSEQKYKDGDGPFLTWEASNRDELLV
                     FTDRQQCYKTRLSEFEDTKASVLGDYLPTKLAMDQEERVVWACIPGDYRRQLLFFFDN
                     GKAARVELSAYQTQTRRKKLTGAYSDKAPLVSAFLLEEDQEMAVYSTEGRCMIFHTSA
                     LSPKTTRTTQGVNV"
     gene            complement(2153..2518)
                     /locus_tag="ACZPAQ_04080"
     CDS             complement(2153..2518)
                     /locus_tag="ACZPAQ_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903194.1"
                     /translation="MKRLTHLFFYTLASIAFLNVFSHSFPSLPALYACMTIFSLLFGV
                     SFLTASGMPSAWTSRKVSSAVWDHWKKILGALFLLNGALCPTGFLLRFDQDLILCTQI
                     FGSLSICFASLIPLYRANA"
     gene            complement(2520..3383)
                     /gene="sdaAA"
                     /locus_tag="ACZPAQ_04085"
     CDS             complement(2520..3383)
                     /gene="sdaAA"
                     /locus_tag="ACZPAQ_04085"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR0903195.1"
                     /translation="MAFDSIAQMLQDTEQSGLPLWENILESDCRRQDLPRARSLERMG
                     AMLDAMVQADESYQAQDRSHSGLSGGDGAKMTLYAAGDPLCGPLLSQVMAGALRMGEC
                     NACMKRIVAAPTAGACGVLPAVLLPCARHFSLSREQLVQALYVASGFGMVIAARASIS
                     GAEGGCQAEIGSAAAMAAPALVHLRGGTPSMMAHACAMAVKNLLGLVCDPVGGLVEVP
                     CVKRNVIGAMDALSAAQMALAGIESRVPPDQVLDAMAEVGRSLPPSLRETGKGGLAAT
                     PFGMAYAPKEG"
     gene            complement(3383..>3850)
                     /locus_tag="ACZPAQ_04090"
     CDS             complement(3383..>3850)
                     /locus_tag="ACZPAQ_04090"
                     /inference="COORDINATES: protein motif:HMM:NF046878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR0903196.1"
                     /translation="DDDRVPGSFALAEERGLSFTFSTRDLRDVHPNSALIRLEDAGGG
                     QLEVGASSPGGGRIRVFQVDGLHTSFSGELPTLVVHNTDQPGCVSQVTGVLAGRGLNV
                     ATLQLNRGGRGGSAVMVIECDQPIPEDAARDVRALPGILRVTRYTPEEQEVPL"
BASE COUNT          684 a         1148 c         1306 g          712 t
ORIGIN      
        1 acgttgaccc cctgggtggt gcgggtagtc ttgggggaga gggcggaggt gtggaagatc
       61 atgcagcgcc cctcggtgga gtagaccgcc atctcctggt cctcctccag caggaaggcg
      121 gagaccaggg gggccttgtc ggaataggcc ccggtgagct ttttccggcg ggtctgggtc
      181 tggtaggcgg acagttccac ccgggccgcc ttgccgttgt caaagaagaa gagcagctgg
      241 cggcggtagt ccccggggat gcaggcccag accacccgct cctcctggtc catggcaagc
      301 ttggtgggca ggtagtcccc cagcacgctg gccttggtgt cctcaaattc gctgagccgg
      361 gtcttatagc actgctgcct gtcggtaaag accagcagct cgtcccggtt ggaggcctcc
      421 caggtaagga aggggccgtc gccgtcctta tacttctgct cgctggacat ccgcagggac
      481 tggggggtga ttttcttcag atatccctcc cgggacagga agagattcac cgggtagtcg
      541 gggacctcct cctcagcggt gagcagctcc tcggtctggt ggtcatagac gatatcggtg
      601 cggcggggga gggcgtactt tttcttgact tgctccagct caccgatgat gacctgacgg
      661 atgcggcggg ggctgttcac caggtcctgg aggtcagcga tctcctcctc cagggagtcc
      721 acctcctggg tgcgcttgag gatgtactcc ttgttgatgt tccgcaggcg gatgtcggct
      781 acgaactcgg cctggatctg gtcaatgcca aagccgatca tcaggttggg cactacctcc
      841 gcgtcctcct cggtctcccg gatgatgcgg atggcccggt cgatgtccag caggatgcgc
      901 ttgaggccct tgagcaggtg cagcttctcc tgcttcttct tcaaggagaa gtatgtgcgg
      961 cggcgcacgc agtcgctgcg ccaggcggtc cactcctcca ggatctcccg cacccccatc
     1021 acccggggca tcccggcgat gaggatgttg aagttgcagg actggctgtc catcaggggg
     1081 gtggacttga acagcttggc catcagcttg tccgggtcag tgccccgctt caggtcgata
     1141 gtcagcttca ggccggacag gtcggtctcg tcccgcatgt cggcgatctc acggatcttg
     1201 cccgacttga tgagctcagc caccttgtcc atgatggcct ccacggtggt ggagtagggg
     1261 atctcgtaga cctcgatgat gttgtccgcc ttgaggtagc gccacttggc gcgcaccttg
     1321 aaggagcccc gtccggtgcg gtagatggag gccagctccg aggcgtcgta gagcagttca
     1381 ccaccggtgg gcaggtcggg ggccttcagg gtggacatga gatcgtggtc cgggtccttc
     1441 agataggcga tggtggtgtc gcacacctcc cccaggttga agccgcacag gttgctggcc
     1501 atgccaacgg cgatgccctg gttggcggag accagcacgt tgggaaaggt ggtgggcagg
     1561 agggtgggct ccttcagctt gccgtcgtag ttgtccacaa agtccacggt gtcctggtcg
     1621 atgtcccgaa acagctcggc gcagatgggg tccagccgca cctcggtgta tcggctggcg
     1681 gcccaggcca tgtctcggga gtacaccttt ccaaagttgc ccttggagtc cacaaggggg
     1741 tggagcaggg ccccgtagcc ccgggagagg cggaccaagg tgtcgtagat ggcggcgtcc
     1801 ccgtgagggt tgagcttcat ggtctggcct actacgttag cgctcttggt acgggccccg
     1861 cccagcagtt tcatctgata catggtgtag agcagcttcc ggtgggaggg cttgaagccg
     1921 tcgatctccg ggatggcccg ggagacgatg accgacatgg catagggcat atagttgatc
     1981 tccagcgtct cggtgatggg ctgctccagc acctcggccc gcaggcccat gacgttgggg
     2041 ttggttacct tgggtttggt ctcgtcgggg tttttcttct ttggcatagg gatatccgtc
     2101 ctatcttctg ccccagctta gggggacatt ttcaatcaaa cactgaaaga gatcacgcat
     2161 ttgcccgata tagagggatc aggctggcaa aacaaatgct caaagatcca aagatttgag
     2221 tacataaaat aagatcctgg tcgaaccgca gaagaaaacc tgtggggcag agcgctccat
     2281 tgagcaaaaa cagagctccg aggatctttt tccagtgatc ccatacggcg gaggaaactt
     2341 tccgacttgt ccaggcggaa ggcattccgc tggccgtcag aaaggataca ccgaataaca
     2401 gagagaaaat ggtcatgcag gcgtacagag cggggagaga gggaaaactg tgtgagaaga
     2461 cattcagaaa agcgatggat gccagtgtat agaaaaagag atgtgtgagc cgtttcatat
     2521 cacccctcct tgggtgcgta cgccatccca aagggcgttg cggccaggcc gccctttccc
     2581 gtctcccgga gggagggggg cagggaacgg cccacctctg ccatggcgtc cagaacctgg
     2641 tccgggggga cccggctctc gatgccggcc agagccatct gggccgccga aagggcgtcc
     2701 atggccccga tgacgttgcg cttgacgcag gggacctcca ccagcccgcc cacaggatcg
     2761 cacaccagcc ccagcagatt cttcacagcc atggcgcagg cgtgagccat catggaggga
     2821 gtgccgcccc ggaggtggac cagggcgggg gcggccatgg cggcggcaga gccgatctcc
     2881 gcctgacagc cgccctcggc cccggagatg gaggcccggg cggcgatgac cattccgaag
     2941 ccggaggcca catacagggc ctggaccagc tgctccctgg agagagagaa gtgccgggcg
     3001 caggggagga gcaccgcggg cagcacgccg caggcccccg ccgtgggggc ggccacgatg
     3061 cgcttcatgc aggcgttgca ctcccccatt cgcagagccc cggccatcac ctgggagaga
     3121 agggggccgc acagggggtc cccggcggcg tacagggtca ttttggcccc gtcacccccg
     3181 gacaggccgc tgtgggagcg gtcctgggcc tgatagctct cgtccgcctg caccatggcg
     3241 tccagcatgg cccccatccg ctccagagag cgggcgcggg gcaggtcctg ccgccggcag
     3301 tcggattcca gaatgttttc ccagaggggc aggccggact gttccgtatc ctggagcatc
     3361 tgggcgatgg agtcaaaggc gatcaaaggg gcacctcctg ttcttccggg gtgtagcggg
     3421 tgacccgcag gataccgggc agggcccgca cgtcccgggc ggcgtcctcg gggatgggct
     3481 ggtcgcactc gatgaccatg acggcgctgc cgccccggcc gccccgattg agctggaggg
     3541 tggccacgtt gaggccccgc ccggccagca cgccggtgac ctgactgacg cagccgggct
     3601 gatcggtgtt gtggaccacc agggtgggca gctccccgct gaaggaggtg tgcagtccat
     3661 ccacctgaaa gacccggatg cgcccgcccc caggggagga ggcccccacc tccagctgtc
     3721 cgcccccggc atcctcaaga cggatgaggg cgctgttggg gtggacgtcc cggaggtccc
     3781 gggtggagaa ggtgaaggac aggccccgct cctccgccag ggcaaagctg cccggcactc
     3841 ggtcgtcgtc
//
LOCUS       DBMDSJ010000122         3846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6120_length_3846_cov_1.954606, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000122 DBMDSJ010000000
VERSION     DBMDSJ010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_3846_cov_1.954606"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /gene="cysS"
                     /locus_tag="ACZPAQ_04095"
     CDS             complement(<1..1049)
                     /gene="cysS"
                     /locus_tag="ACZPAQ_04095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR0903197.1"
                     /translation="MLLYNSATHKKEEFQTHTPGHVEMYTCGPTVYHFAHIGNLRSYI
                     MEDVLEKFLRWSGYDVNRVMNITDVGHLSSDADTGEDKMLKGARREHKTVMEIAQYYT
                     DAFFEDCRKLNIKRPDTVQPATGCIDEYIQIITRLLEKGYAYQAGGNVYFDSSKLERY
                     YVFNDHDEEDLAVGVRDGVEEDTNKRNKNDFVLWFTKSKFEDQALKWDSPWGVGYPGW
                     HIECSGISMKYNGEYLDLHCGGIDNAFPHHTNEIAQSESYLGHPWCRQWFHVHHLNTN
                     SGKMSKSSGEFLTVSLLEEKGYDPLVYRFFCLQSHYRKGLVFSWENLDNARAAFDKLI
                     ARIAALDPQDGSVDEA"
     gene            complement(1111..1764)
                     /gene="epsC"
                     /locus_tag="ACZPAQ_04100"
     CDS             complement(1111..1764)
                     /gene="epsC"
                     /locus_tag="ACZPAQ_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR0903198.1"
                     /translation="MFQSLSELLGAYQKRDPAARSKLEIFLLYPGVHAVIFHRVSHWL
                     YCHHLPFLARLNSQLARHITGIEIHPGAKIGRRLVIDHGMGIVIGETAEIGDDCLIYH
                     GVTLGGTGKDHGKRHPTIGNNVLISCGAKVLGPFRVGDNARIASNAVVLSEVPEDATA
                     VGIPAQIVRIAGRVTHYADDVDQTSVENPTLERLEALSQRVEFLEKLLDDQFLKERA"
     gene            complement(2236..3183)
                     /locus_tag="ACZPAQ_04105"
     CDS             complement(2236..3183)
                     /locus_tag="ACZPAQ_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014542.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family putative permease subunit"
                     /protein_id="HIR0903199.1"
                     /translation="MKDPRIPLYLITGFLDSGKTTLLTDTLNMDYFADGQRTVLLMCE
                     DGETEYDPEQLTRRNTRLVPVQDQAQLNTSFLQEIDRRYQPERVLLEYNGMWPIQILR
                     DLKLPKTWGLYQAIDVIDGSTFEMYRSNMQSMVIDMVTEADMVLFNRCTPDMPLSGWK
                     RSIRAVNRMCEIVFEDEKGEELEVEDILPYSLEADTIVLEDEDYGIWYLDIQEHPERY
                     TGKTVVFRAQAMTSMKLPKGTFIPGRNAMTCCAEDIRFFGFLCKYDRSRSLKKGEWVM
                     VAARIQWEESPVYQGEGVVLYAQRVERADRPDDPLVYFR"
     gene            complement(3195..>3846)
                     /locus_tag="ACZPAQ_04110"
     CDS             complement(3195..>3846)
                     /locus_tag="ACZPAQ_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140827 - zinc chaperone activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobW C-terminal domain-containing protein"
                     /protein_id="HIR0903200.1"
                     /translation="NAKAAILTTPWDQLTGAQIVSAMEGGHDLARELMEEIGHHHDHG
                     EDEPCSCGHDHHHDHEHKHEHEHEHHHDHDHGDSCSCGHHHTQEELDAIHAELGCEGA
                     CGSCDLCDSCIHSTFHHHHHADEVFTSWGRETPRKYSREELQDILSVLAMTEDYGTIL
                     RSKGMVPDQDSEGWLHFDLVPGEFQIREGSADYTGRLCVIGAGLDEARLEKLFLLA"
BASE COUNT          667 a         1126 c         1281 g          772 t
ORIGIN      
        1 gcttcgtcca cagatccgtc ctgggggtcc agggcggcga tgcgggcgat gagcttgtca
       61 aaggcggccc tggcgttgtc caggttctcc caggagaaca ccagcccctt gcggtagtgg
      121 ctctggaggc agaagaaccg gtagaccagg ggatcatagc ccttctcctc cagcagggag
      181 acagtgagga attcgccgga ggacttggac atcttgccgg aattggtgtt caggtggtgg
      241 acatggaacc actggcggca ccaagggtgg cccagatagg actcggactg ggcgatctca
      301 ttggtgtggt gggggaaggc gttgtcgatg ccgccgcagt gcaggtccag atactccccg
      361 ttgtacttca tggagatgcc ggaacactcg atgtgccagc cggggtagcc cacgccccag
      421 ggggagtccc acttcagggc ctgatcctcg aatttggact tggtgaacca gaggacgaag
      481 tcatttttat tgcgcttgtt ggtgtcctcc tccacgccat cccggacgcc caccgccaga
      541 tcctcctcat cgtggtcgtt gaacacgtag tagcgctcca gcttggagga atcgaagtac
      601 acattgcccc cggcctgata ggcgtagccc ttctccagca ggcgggtgat gatctggata
      661 tactcgtcga tgcagccggt ggcgggctgc accgtgtcag ggcgcttgat gttcagcttt
      721 cggcagtcct cgaagaaggc gtcggtgtaa tactgggcga tctccatcac ggtcttgtgc
      781 tcccggcggg cgcccttgag catcttgtcc tcgccggtgt cagcgtcgga gctgaggtgg
      841 ccaacgtcag tgatgttcat cacccggttc acgtcatagc cggaccagcg caggaatttt
      901 tccagcacgt cctccatgat gtaggagcgc aggttgccga tgtgggcgaa gtggtacacc
      961 gtgggtccgc aggtgtacat ctccacgtgg ccgggggtgt gggtctggaa ctcctccttc
     1021 ttgtgggtgg cggaattata taacagcatg ataaaaaacc tccattttct tcggtgaggg
     1081 cggcctgggc cgcccgggat cgtgagagaa ctatgcccgt tccttcagga actggtcatc
     1141 cagcagcttc tccaggaatt ccacacgctg agacagggct tccagccgtt ccagggtggg
     1201 attctccacg ctggtctggt ctacgtcatc ggcgtagtgg gtgacccgtc cggcgatgcg
     1261 gacgatctgg gcggggatgc ccacggcagt ggcgtcctcc ggcacctcgc tgagcaccac
     1321 ggcgttggac gcgatccggg cgttgtcacc cacccggaag ggccccagca ccttggctcc
     1381 gcagctgatg agcacgttgt ttccgatggt gggatggcgc ttgccgtggt ccttgccggt
     1441 gccgcccagg gtgacgccat gatagatcag gcagtcgtcg ccgatctcgg cggtctcgcc
     1501 gatgacgatg cccatcccgt ggtcaatgac caggcggcgg ccgatcttgg cgccggggtg
     1561 gatctcaatg ccggtgatgt ggcgggccag ctgggagttc aggcgggcca ggaagggaag
     1621 gtggtgacag tagagccagt gggagacgcg gtggaagatg acggcgtgga cgccggggta
     1681 gaggagaaag atctccagct tgctgcgggc ggccgggtcg cgcttctgat aggcgcccag
     1741 caattcactc agtgactgaa acatgatgtc ctccttgggg gagatccatt ccgtctcccc
     1801 atttattgtg ggcgggaaga acagcacccg ggacatcgcg gtgcgctccg gcggctgtgc
     1861 cggagccggg gaaagggaag atgcaagggg aacgcctcct tttcgcagct tggacacaaa
     1921 caaaagcgcc cctcatgccc acaggcataa gaggcgatcg ctcgcggttc cactctcatt
     1981 tttcactcaa aggccggtat cggggccaga ccgggcggca ttacccgccg cgctcccgga
     2041 cgcacttcac atcccgcgga ctgggaccgc tctcagtcgg tggcgttccc tctctgtcga
     2101 ccggtggagg atgctacttt tccgttcaca gcgcttacaa tttattatac tatcatctga
     2161 aggaaggggt gtcaaggagg agggagggct tttcaaaaat tttcccgacg ccccgccggg
     2221 gcgccgggaa aagggtcatc ggaaatagac caggggatcg tccgggcggt cggcccgctc
     2281 cacccgctgg gcgtagagga ccacgccctc cccctgatag acgggggact cctcccactg
     2341 gatgcgggcg gccaccatga cccattcccc cttcttcagg gagcgggacc ggtcatactt
     2401 gcacaggaag ccgaagaagc ggatgtcctc ggcgcagcag gtcatggcgt tgcggccggg
     2461 gatgaaggtg cccttgggca gcttcatgct ggtcatggcc tgggcccgaa agaccacggt
     2521 cttgccggtg tagcgctctg ggtgctcctg gatgtccaag taccagatgc cgtagtcctc
     2581 gtcctccagg acgatggtgt ccgcctccag ggagtagggg aggatgtcct ccacctccag
     2641 ctcctccccc ttctcgtcct caaagacgat ctcgcacatg cggtttaccg cccggatgga
     2701 gcgcttccag ccggacaggg gcatgtccgg cgtgcagcgg ttgaagagga ccatatcggc
     2761 ctcggtgacc atgtcgatga ccatggactg catgttggag cggtacatct cgaaggtgga
     2821 gccgtcgatg acgtcgatgg cctggtagag cccccaggtc ttgggcagct tcaggtcccg
     2881 gaggatctgg atgggccaca tgccgttgta ctccaggagc acccgctccg gctgatagcg
     2941 gcggtcgatc tcctggagaa agctggtgtt cagctgggcc tggtcctgga cagggaccag
     3001 gcgggtgttc cgcctggtca gctgctccgg atcatactcc gtctcgccgt cctcacacat
     3061 cagcaggacg gtgcgctgcc catcggcaaa ataatccata ttcagggtgt cggtgagcag
     3121 agtggtcttg ccgctgtcca gaaagccggt gatgaggtac agcgggatac ggggatcttt
     3181 catgcagcct cacctcacgc cagcaggaac agcttttcca gcctggcctc gtccaggccc
     3241 gcgccgatga cgcacaggcg gccggtgtag tcggcgctgc cctcccggat ctggaactcg
     3301 ccggggacca ggtcgaagtg gagccagccc tcgctgtcct gatcggggac catgcccttg
     3361 gagcggagga tggtgccgta atcctccgtc atggccagga cggaaagaat gtcctggagt
     3421 tcctcccggc tgtacttccg aggagtctcc cggccccagc tggtgaacac ctcgtcggca
     3481 tggtgatggt ggtggaaggt ggagtggatg cagctgtcgc acaggtcgca ggagccgcag
     3541 gccccctcgc agcccagctc agcgtggatg gcgtccagct cctcctgggt gtggtgatgg
     3601 ccgcaggagc aggagtcccc gtggtcatgg tcgtggtggt gctcgtgctc atgctcatgc
     3661 ttatgctcgt ggtcatggtg gtggtcatgg ccgcaggagc agggctcgtc ctccccgtgg
     3721 tcgtggtggt gtccgatctc ctccatcagc tcgcgggcca agtcgtggcc cccctccatg
     3781 gcagagacga tctgggcgcc ggtgagctgg tcccaggggg tggtgaggat agccgccttg
     3841 gcgttg
//
LOCUS       DBMDSJ010000123         3827 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6146_length_3827_cov_1.695115, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000123 DBMDSJ010000000
VERSION     DBMDSJ010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6146_length_3827_cov_1.695115"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="ACZPAQ_04115"
     CDS             complement(<1..241)
                     /locus_tag="ACZPAQ_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903201.1"
                     /translation="MSTVKTRGAKGAREPREPKLGVLGGMGPQATQVFYQYVLDRTEA
                     SCDQEHLPALILSDTGVPDRTASILTGNTEPMYQRL"
     gene            complement(260..1477)
                     /locus_tag="ACZPAQ_04120"
     CDS             complement(260..1477)
                     /locus_tag="ACZPAQ_04120"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR0903202.1"
                     /translation="MSRTVSFQPLLFGGDINVYSVARAFHEAYGVKSVAFGKFHTFPC
                     AFSSIIDYRTCPDNEDPAVFLSNVEKVALEFSHKTVIVLGCGDSYVQLCAHLKDQFPR
                     NVVAPYIDGALLDRLINKEHFYELCDQHGIDHPATFIYDGSMGHDFTLPFGPPYVAKP
                     ADSVAYWEHEFEGVKKVFICQTWEELLHALDLVYASGYPDHMVIQEFIPGDDTYMRVM
                     TNYSDRSGQVKLMCLGHVLLEEHSPHGIGNHAVIITEYDEALSLKIKQLLEELHYTGF
                     SNFDIKYDSRDGKYKLFEINCRQGRSNYYVTGAGYNIAKLLVEDRIEHRELPFVITKN
                     HALWRMVPKKVAFKFIPKAYHPQMKALIRAGASTHSLEYHRDYTPARIWRVWKTHIGN
                     MVRFDRHNKVPEN"
     gene            complement(1512..2576)
                     /locus_tag="ACZPAQ_04125"
     CDS             complement(1512..2576)
                     /locus_tag="ACZPAQ_04125"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR0903203.1"
                     /translation="MRVLVIEKAAVKQNIRVVKRRAGGAAVYGVLSGDAGGAGIAEMA
                     ELLRAEGIGRFAVSEAEEAEALRKAGFVDEEILMLRSTTDREVLERLVDLNVVCTISS
                     VDTGLALNALAENRSTVAEAHIQVDTGMGFGGFLVGEPEKILLAYRSLPNVALSGIYT
                     QIHAAPAGQEAEHQLQQFQRVLDAIHAAGFETGTVHAAGSYALMHCDLARMDAVRAGS
                     VLLGRCRRTKDDGLSTVGHGEAGIAEMRWLPKGHTVGAEKTAVLKAPTRVAVVPVGYQ
                     HGFGVERPRPAGLLGTLGRWRRGRRLYVRVNGQRARVIGSVGVSETLVDVTDLKCSAG
                     DVVSFDLDPMYARGCVRQYR"
     gene            2880..>3827
                     /locus_tag="ACZPAQ_04130"
     CDS             2880..>3827
                     /locus_tag="ACZPAQ_04130"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR0903204.1"
                     /translation="MRQQTTRISPEDISRQLQYCREVAALSAARPAQPLAFVDTYGCQ
                     QNEADSERIRGYLREMGYGFTQDEEQAQIIVLNTCAIREHAEQRVLGNLGALVHVKRR
                     HPEQIICVCGCMAQEAHVAEKIKQSFRHVDLVFGPHALWRFPEFIHTLLTQRGRVFQV
                     AEEAGSISEGIPVVRQDRVKAWVSIMYGCNNFCSYCIVPYVRGRERSRRPEDILAEVE
                     QLAAEGYRDITLLGQNVNSYGKDLEEPLDFADLLERVNAVPGDFLIRFMTSHPKDATQ
                     KLFETMARCGKVAPVLHLPFQAGNDRVLRAMNRHYDRAQY"
BASE COUNT          662 a         1220 c         1274 g          671 t
ORIGIN      
        1 gcaggcgctg gtacataggc tcggtgttcc cggtgaggat ggaggcggtc cggtccggga
       61 ccccggtgtc agacaggatc agggcgggca gatgctcctg gtcgcaggag gcctctgtcc
      121 ggtccagcac gtactgatag aacacctggg tggcctgggg ccccatccca cccagcacgc
      181 ccagcttggg ctcccggggc tctctggcgc ccttggcgcc gcgggtcttg acagttgaca
      241 tggcttccct ccagtctgct cagttctccg ggaccttgtt gtggcggtca aagcggacca
      301 tgttgccgat gtgggtcttc catacccgcc agatgcgggc cggggtatag tcccggtggt
      361 actccaggct gtgggtggac gcccccgccc ggatcagggc cttcatctgg gggtgatatg
      421 ccttggggat gaatttgaag gccacctttt tgggcaccat ccgccacagg gcgtggtttt
      481 tggtgatgac gaagggcagc tcccggtgtt cgatccggtc ctccaccagc agcttggcga
      541 tgttgtatcc cgcgccagtg acgtagtaat tgctgcgccc ctggcggcag ttgatctcga
      601 agagcttgta cttgccgtcc cggctgtcgt acttgatgtc aaagttggag aagccggtgt
      661 aatgcagctc ctccagcagc tgtttgatct tcagggaaag ggcctcgtcg tactcggtga
      721 tgatgacggc atggttgccg atgccgtggg gggagtgctc ctccagcagc acatggccca
      781 ggcacatcag cttcacctgg ccgctgcggt cggagtagtt ggtcatcacc cgcatgtagg
      841 tgtcgtctcc ggggatgaac tcctggatca ccatatggtc ggggtagccg gaggcgtaca
      901 ccaggtccag agcgtggagc agctcctccc aggtctgaca gatgaagacc ttcttcaccc
      961 cctcgaattc gtgctcccag taggccacgc tgtcggccgg cttggccaca tagggggggc
     1021 cgaagggcag ggtgaagtcg tggcccatgg agccgtcgta gataaaggtg gccgggtggt
     1081 cgatgccgtg ctggtcgcac agctcgtaga agtgctcctt gttgatgagg cggtccagca
     1141 gggcgccgtc gatgtagggg gccaccacgt ttcggggaaa ctggtccttc aggtgggcgc
     1201 acagctgcac atagctgtcc ccgcagccca ggacgatgac ggtcttgtgg gagaactcca
     1261 gggccacctt ctctacattg gagaggaaga cggcggggtc ctcgttgtcg gggcaggtgc
     1321 ggtagtcgat gatggagctg aaagcacagg ggaaggtgtg gaacttgccg aaggccacgc
     1381 tcttcacccc ataggcctcg tgaaaggcac gggccacact gtaaacattg atatcgccgc
     1441 cgaagagcag cggctgaaaa gacacggttc tggacatagc aaacaccttt ttcttgaaat
     1501 cgcagggagg atcaccggta ctggcggaca cagccccggg cgtacatggg gtccaggtcg
     1561 aaggagacca catctccggc ggagcacttc agatcggtga catccaccag ggtctcgctc
     1621 acgcccacag agccgatgac ccgggcccgc tggccgttca cccggacata aagccgcctg
     1681 ccccgccgcc agcggcccag ggtgcccagc aggccggcgg gacggggccg ctccaccccg
     1741 aagccgtgct gatagcccac agggaccacc gctacccggg tgggcgcttt caaaacggcg
     1801 gtcttttccg cacccacggt gtgccccttg ggcagccagc gcatctcggc aatgccggcc
     1861 tccccgtggc ccacggtgga gagcccgtcg tctttggtgc ggcggcagcg gcccagcagg
     1921 accgagcccg cccgcaccgc gtccatgcgg gcaagatcgc agtgcatcag ggcgtaggac
     1981 cccgcggcgt ggacggtgcc ggtctcaaag ccggcggcgt ggatggcgtc cagcacccgc
     2041 tggaactgct ggagctggtg ctccgcctcc tggccggctg gggcggcgtg gatctgggtg
     2101 taaatgccgg acagggccac gttgggcagg gagcggtagg ccagcaggat cttctccggc
     2161 tcccccacca ggaagccgcc gaagcccatg ccggtgtcca cctggatgtg ggcctcggcc
     2221 acggtggagc ggttctccgc cagggcgttg agggccaggc ccgtgtccac ggaggagatg
     2281 gtgcacacca cgttcaggtc caccagccgc tccagcacct cccggtcggt ggtggagcgg
     2341 agcatgagga tctcctcgtc cacaaagccg gccttccgca gcgcctcggc ctcctccgcc
     2401 tcgctgacgg cgaagcggcc gatgccctcg gcccggagca gctcggccat ctcggcgatg
     2461 ccggcgcccc cggcgtctcc cgagaggacg ccgtagacgg cggcccctcc ggcacggcgc
     2521 ttcaccaccc tgatgttctg ctttacggcc gccttctcaa tgaccagcac ccgcatggca
     2581 cagccccctt tttctctctg ttgggaagga gagagatacg tctccctcca gtgtaaccat
     2641 ttaacgattc attctatcac ccttttgaga gaaacgcaac ggaaaaccgc gagggggccc
     2701 gcatttttcc agggaggggc ggagagccgc gccctgcggg gaccgggtgg aaaagcggcg
     2761 aaaagcaaaa gtccggcctt gtgctttttc gggaaaaatg gtatgattat tcgatcggtg
     2821 tctccatgca gcaggagggg ccggacgacc atcaaatccc cctcaggagg ttgcgatcta
     2881 tgagacagca gaccacccga atttccccgg aggacatctc ccgccagctc cagtactgcc
     2941 gggaggtcgc ggccctcagc gccgcccggc ccgcccagcc cctggccttt gtggacacct
     3001 acggctgcca gcagaacgag gcggactccg agcgcatccg gggctatctc cgggagatgg
     3061 gctacggctt cacccaggac gaggagcagg cccagatcat cgtgctcaac acctgcgcca
     3121 tccgggagca cgcagagcag cgggtgctgg gcaacctggg cgccctggtc cacgtgaagc
     3181 gccgccaccc ggagcagatc atctgcgtgt gcggctgcat ggcccaggag gcccatgtgg
     3241 cggagaagat caagcagtcc ttccgccacg tggacctggt gttcgggccc cacgccctct
     3301 ggcgctttcc cgagttcatc cacaccctgc tcacccagcg ggggcgggtg ttccaggtgg
     3361 cggaggaggc gggctccatc tccgagggca tcccggtggt gcggcaggac cgggtaaagg
     3421 cctgggtgtc catcatgtac gggtgcaaca acttctgctc ctactgcatc gtgccctatg
     3481 tgcggggccg ggagcgctcc cgcaggccgg aggacatcct ggccgaggtg gagcagctgg
     3541 cggcggaggg gtaccgggac atcaccctgc tgggccagaa cgtgaactcc tacggcaagg
     3601 acctggagga gcccctggac ttcgcagacc tgctggagcg ggtgaacgcc gtgcccgggg
     3661 acttcctgat ccgctttatg acctcccacc ccaaggacgc cacccagaag ctgttcgaga
     3721 ccatggcccg gtgcgggaag gtggccccgg tgctccacct gcccttccag gcgggcaatg
     3781 accgggtgct ccgggccatg aaccgccact atgaccgggc gcagtac
//
LOCUS       DBMDSJ010000124         3827 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6147_length_3827_cov_1.424852, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000124 DBMDSJ010000000
VERSION     DBMDSJ010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_3827_cov_1.424852"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(137..634)
                     /locus_tag="ACZPAQ_04135"
     CDS             complement(137..634)
                     /locus_tag="ACZPAQ_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR0903205.1"
                     /translation="MKKIDVKSLQDNVFSLIGDRWMLITAGTAERCNTMTASWGGLGV
                     LWGAPAATIYLRPQRYTKEFVDREEYFTLAFFGEEQRKALTLCGSKSGREVDKVKECG
                     FTVAQAECGAPYFEEAELVLVCRKRYAQEMDPQAIPQDAKDRFYPEKDYHTMYIGEVV
                     EALRK"
     gene            complement(769..972)
                     /gene="rpmE"
                     /locus_tag="ACZPAQ_04140"
     CDS             complement(769..972)
                     /gene="rpmE"
                     /locus_tag="ACZPAQ_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR0903206.1"
                     /translation="MKAGIHPDYQQTTIRCACGNVIETGSTKKDIRVEVCSKCHPFYT
                     GKQKMVDTGGRVSRFNKKFGLDK"
     gene            complement(1106..>3827)
                     /locus_tag="ACZPAQ_04145"
     CDS             complement(1106..>3827)
                     /locus_tag="ACZPAQ_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR0903207.1"
                     /translation="RKKGVEDLEAAERVKGLRTRCKKQMDKLFEDLGGDSGQLLADLA
                     LARPAVRGLMELTARFQEDYAREKARRGILDFSDLEHLALRLLTQDGDGGPGPLARYW
                     SGRFAEVMVDEYQDTNQVQNAIFTAVSDGGRKLFMVGDVKQSIYRFRLADPTIFLDKY
                     RRFKPWDQAAEGEERTVVLTRNFRSRPEVLEGTNDLFRNIMSTEFGELDYTQDQALAP
                     GAAFPPEGDWRVELDLEDLSFLGDQEEGERADKNLLEARWAARRIRELLDGELMLTEG
                     SGQRRAEPSDVLLLLRSPGAVLHHYIRALNEAGLPWSAEGGEDFFASTEINVALSLLQ
                     IVDNPRQDVALIAALRSPVYGFSGDQLALLRAEGEGDFYSAVVQAAGRGDQACRDFLT
                     ELEELRFGAGDRTCRQLIWHIYERTNLLGLFGAMDGGQERQGRLLTLYALAGRLEEAG
                     CRSLFQFLLRLERMRQTGGLSLSAPGREGGGVTIMSIHRSKGLEKPVVLLCGLSRRLN
                     RDDLQRPVLFHPVLGVGPRGLDRERMVEYPTLARKAVARKLEREMMAEELRLLYVAMT
                     RAKEKLILSIPLRRGAEKLEKLGEDLSVPPSPMALERQQSVGGWVLLHALARPEASAL
                     RSMAGLPEVSPGTLGPAWDIRWVDGAALAQIPEQRGRFADLPGESETEPEGLREALAW
                     HYPFSLSSQTPSKLTATQLKGRSLDQETREEAAPAALPPQPLSILRPQFVAEERGLTP
                     AQRGTALHLAMQYLPLDTDTPERAAAELERLVSGGFLTPQQGAAADPGQLSAFFNSPL
                     GREMASAPVCRREFKFSVLLPAEEFCPGLEPGEEVLLQGVVDAWFETLEGITVVDFKS
                     DRVSRSGAAARGEEYRPQLEAYSRALEEITGKQVVRKALWFFEPGELVLL"
BASE COUNT          634 a         1381 c         1137 g          675 t
ORIGIN      
        1 tcctccaggg ccaggacgcg ggaggacagg cccaccagca ccgccctgtt tttcttttcc
       61 gtgttgtttt ctgtcataga tcctccaaaa tgggattttc cccgtctgtc aggaggcggt
      121 ccgggcgctg aggcccttat ttcctcagcg cctccacgac ctctccgata tacatggtat
      181 ggtagtcctt ctcaggataa aatctgtcct tggcatcctg ggggatggcc tgggggtcca
      241 tctcctgggc atagcgcttg cggcagacca gcaccagctc cgcctcctca aaataggggg
      301 ccccgcactc ggcctgggcc acggtgaagc cgcactcctt caccttgtcc acctcccggc
      361 cgctcttgct gccgcacagg gtgagggcct tgcgctgctc ctccccgaaa aaggccaggg
      421 tgaaatactc ctcccggtcc acgaactcct tggtgtaccg ctggggccgc aggtaaatgg
      481 tggccgccgg ggcgccccac agcacgccca gtccgcccca gctggcggtc atggtgttgc
      541 accgctccgc cgtgccggcg gtgatgagca tccaccggtc cccgatgagg gaaaatacgt
      601 tgtcctgcaa gctcttcacg tcgatcttct tcatgataca gcctcctaac tcatttttga
      661 tctcatctgt tagtttatgc ccctccctgg ggcgaggata ctccgggccg acagcaaaat
      721 acgacaaaaa gccgcaccga tccacggtgc ggcttttgag gcgcaatctc acttgtccag
      781 gccgaacttc ttgttgaagc ggctgacacg accaccggtg tccaccatct tctgcttacc
      841 ggtgtagaag gggtgacact tggaacacac ttccacgcgg atatccttct tggtggagcc
      901 agtctcgatc acattgccgc aagcgcaccg gatggtggtc tgctgataat cgggatggat
      961 gcctgctttc attgtatctt cacctctttc tccatggagg gatcatctgt aaacgcaact
     1021 gatataatag catggatctc ccgcaaaagc aactattttt tcaaaaaatt atcctgactt
     1081 ttttctcggc ccttttgtcg ccctttcaca gaaggaccag ctcccccggt tcgaagaacc
     1141 agagcgcctt tcggaccacc tgttttccgg tgatctcctc cagggcccgg ctgtacgcct
     1201 ccagctgagg gcggtactcc tctccccggg ccgccgcccc ggaccgggag acccggtcgc
     1261 tcttgaagtc caccaccgtg atgccctcca gagtctcgaa ccaggcgtcc actacgccct
     1321 ggagcagcac ctcctccccc ggctccaggc cggggcagaa ctcctccgcc ggcagcagca
     1381 cggaaaattt gaattcccgg cggcacacgg gggccgacgc catctccctg cccaggggcg
     1441 agttaaagaa cgcggacagc tgtcccggat cggccgccgc cccctgctgg ggggtgagga
     1501 agccgccgga caccaggcgc tccagctccg ccgctgcccg ctccggggtg tccgtgtcca
     1561 gggggagata ctgcatggcc aggtgcaggg ctgtgccccg ctgggccggg gtgagtcccc
     1621 gctcctccgc cacgaactgg ggccgcagga tggagagcgg ctggggcgga agggccgccg
     1681 gggcggcctc ctcccgggtc tcctggtcca ggctccgccc cttgagctgg gtggcggtga
     1741 gcttggaggg ggtctgggag gaaagggaga acggatagtg ccaggccagc gcctctctca
     1801 gcccctccgg ttccgtctcc gactctccgg gcagatccgc gaagcggccc ctctgctccg
     1861 gaatctgggc cagtgccgcc ccatctaccc atcgaatatc ccaggcgggc cccagagtcc
     1921 ccggggacac ctccggcagc cccgccatgc tccgcagggc ggacgcctcg ggccgggcca
     1981 gggcgtgaag cagcacccag ccgcccacgc tctgctggcg ctccaacgcc atgggggagg
     2041 gtggaacaga caggtcctcc cccagcttct ccagcttctc cgctccccgc cgcagcggga
     2101 tggagaggat cagcttctct ttggcccggg tcatggccac atagagcaga cgcagctcct
     2161 ccgccatcat ctcccgctcc agcttccggg ccaccgcctt gcgggccagg gtgggatact
     2221 ccaccatccg ctcccggtcc aggccccggg ggcccacccc cagcaccgga tggaagagga
     2281 caggccgctg gaggtcgtcc cggttcagcc gccgggacag gccgcacagc agcaccaccg
     2341 gtttctccaa ccccttggag cggtggatgg acatgatggt cacaccgccc ccctcccggc
     2401 ctggggcgga cagggacagt cccccggtct ggcgcatccg ctccaggcgc agcaggaact
     2461 ggaagagaga ccggcacccc gcctcctcca gacgccccgc cagggcatag agggtgagca
     2521 ggcggccctg ccgctcctgc cccccgtcca tggccccgaa caggcccagc aggttggtcc
     2581 gttcatagat gtgccagatc agctggcggc aggtgcggtc ccctgccccg aagcgcagct
     2641 cctccagctc cgtcaggaaa tcccggcacg cctggtcccc ccggccggcg gcctgaacca
     2701 cagcggaata gaagtccccc tccccctccg cccggagcag agccagctga tctcctgaaa
     2761 agccgtacac cggggagcgc agggccgcga tcagcgccac atcctggcgg ggattgtcca
     2821 cgatctggag aagggacagg gccacattga tctcggtgga ggcaaagaag tcctccccgc
     2881 cctcggcgga ccagggcagc cccgcctcgt tgagggcccg gatatagtgg tggagcaccg
     2941 ccccagggga gcgcaggaga aggagcacgt cagagggctc cgcccgccgc tgcccgctcc
     3001 cctccgtcag catcagctcc ccgtccagca gctcccggat acggcgggcc gcccagcggg
     3061 cctccagcag atttttgtcc gcccgctccc cttcctcctg gtcccccagg aaggacaggt
     3121 cctccaggtc cagctccacc cgccagtctc cctccggcgg gaaggcggct cccggcgcca
     3181 gcgcctggtc ctgggtgtag tccagctccc cgaattcagt tgacataata tttcggaaga
     3241 ggtcgttggt cccctccagg acctccggcc gggaacggaa gttgcgggtg agcaccactg
     3301 tccgctcctc cccctccgcc gcctggtccc aaggtttgaa acggcggtac ttgtccagga
     3361 agatggtggg gtcggccagc cggaagcggt agatggactg cttcacgtct cccaccataa
     3421 acagcttccg ccccccatcg gacacggcag tgaagatggc gttctgcacc tggttggtgt
     3481 cctgatactc gtccaccatc acctcggcga accggccgct ccagtaccgg gccagcgggc
     3541 cgggccctcc gtccccgtcc tgggtgagca gccgcagggc cagatgttcc aggtcggaaa
     3601 agtccagtat gccccggcgc gccttctccc gggcgtagtc ctcctgaaag cgggccgtca
     3661 gctccatcag gccccgcacc gcgggccggg ccagggccag atcggccagc agctgcccgc
     3721 tgtccccgcc caggtcctca aagagcttgt ccatctgctt cttgcagcgg gtgcgcagtc
     3781 ccttcacccg ctccgccgcc tccaggtcct ccacgccctt tttccgc
//
LOCUS       DBMDSJ010000125         3821 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6155_length_3821_cov_1.656183, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000125 DBMDSJ010000000
VERSION     DBMDSJ010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6155_length_3821_cov_1.656183"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="ACZPAQ_04150"
     CDS             <1..499
                     /locus_tag="ACZPAQ_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR0903208.1"
                     /translation="NMDLIAGLPQDSVEGFRDSLEEVLALRPENITVHTLAMKKGSRL
                     MEEGGALPSSEETGRMVNLSLEALEGAGYRPYYLYRQKYMSGGLENVSWCLPGTENHY
                     NIIMMEELQSVLSLGAGGVTKLVDRSSGRIVRLTNPKYPHDYLAMSAKVLEQKDEILR
                     FYREV"
     gene            504..1442
                     /locus_tag="ACZPAQ_04155"
     CDS             504..1442
                     /locus_tag="ACZPAQ_04155"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR0903209.1"
                     /translation="MAYQLQEINRRIKTDVTEFLEECDQAYAQRVSLAADRILSNLDK
                     SPVVLLSGPSGSGKTTTAMKIAEELQRRGVNTHAVAMDNYFKSLNRKTAPRTPEGDID
                     YESPQCLDMELLDQHFAALSRGEEIIIPKFEFARQMRNDARGTPLKLGKNEIAIFEGI
                     HALNDDIAGPHPEAAKLYISARSNVNDGAALRFKGTWMRLTRRAVRDYSFRGTGVADT
                     LEMWANVRRGEKLYISPYKNRADIIFDSSLPYEVSVMKNFALPLLQAVPEENARRGEL
                     LELIEAFQWFEPIDPELVPVDSLLREFIGGGSYKYH"
     gene            1527..3401
                     /locus_tag="ACZPAQ_04160"
     CDS             1527..3401
                     /locus_tag="ACZPAQ_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR0903210.1"
                     /translation="MSDPLVYDSRSVRFKSPYGAVPSGTQVTFTLRPLRSEGYSRGKL
                     TARLEQRDNQIITVELPWTDTELGRDAFSGVLDTGDYVGLVWYTFQLETITGRRWNIK
                     EEYQLTVYDGSEIVPRWFGEGVSYQIFPDRFRRTRVPDPAGLVGGRTVHQSWQEEPEY
                     RPDANGEIRNRDFFGGDLRGVIEELDYLQSLGVETLYFNPIFEAAENHRYGTADYSRV
                     DPMLGTNEDFSELCRQAHRRGMRVMLDGVFNHTGFVSRYFNGDGYYPEPGAVQSESSP
                     YRPWFQFRHWPDQYESWWGIYTLPAVEESCPGYREFIFGDENSVVRRWLRAGADGWRL
                     DVADELPDDFVAGIHTAARAEKPGALLIGEVWEDGTTKIAYGVRRKHILGRHCDGLMN
                     YPFRNAALNFLRGGDGAKFVSAMEALRENYPSFAFYSAMNSLGTHDTPRIITLLGVGS
                     ECRDQSKDWRACFRMSMEQYQRGKELLKVGAVLLYAFPGSPTVYYGDEAGLEGFEDPF
                     NRRPYPWGREDRELVGWFAALGRLRKECPALRRGDIRYLAGTGHLLAFLRSTEGERLL
                     TALNAGDEPAVLALPWTEEPERLLGAGEWALTGEGPVLMLPPRSGGIWRVGEAEPAQM
                     "
     gene            3545..3775
                     /gene="secG"
                     /locus_tag="ACZPAQ_04165"
     CDS             3545..3775
                     /gene="secG"
                     /locus_tag="ACZPAQ_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR0903211.1"
                     /translation="MKTAVTVIQVLCGLFLIAVVLLQSGKSAGLSGAIAGGADTFLSK
                     NKAKTVDAKLAKWTKWVAIAFMLLTLSLSLIP"
BASE COUNT          718 a         1136 c         1289 g          678 t
ORIGIN      
        1 caacatggac ttgatcgccg gcctgcccca ggacagcgtg gagggcttcc gggactctct
       61 ggaggaggtg ctggccctga ggcctgaaaa catcaccgtc cacaccctgg ccatgaaaaa
      121 ggggtccagg ctgatggagg aggggggcgc gcttccttcc agtgaggaga cgggccgaat
      181 ggtgaacctc agcctggagg ccctggaggg ggcaggctac cggccctatt acctctaccg
      241 gcagaagtac atgtcaggcg ggctggaaaa tgtgagctgg tgtttaccgg gaacggaaaa
      301 ccactataat atcatcatga tggaggaact gcagagcgtc ctgtccctag gggctggcgg
      361 ggtcaccaag ctggtggacc ggagttcggg gcggatcgtc cgcctcacca accccaagta
      421 tccccacgac tatttggcga tgtccgccaa agttctggag cagaaggacg agatcctgcg
      481 cttctaccgg gaggtttgaa ccaatggcct atcaacttca ggagatcaac cgaaggatca
      541 agaccgatgt caccgagttt ctggaggagt gcgaccaggc gtatgcccag cgggtgtccc
      601 tggccgcaga ccgcatcctg agcaatctgg acaagagccc cgtggtgctg ctctccgggc
      661 cctcgggctc gggcaagacc accaccgcca tgaagatcgc ggaggagctc cagcgccggg
      721 gtgtcaatac ccacgcggtg gctatggaca actacttcaa gagcctgaac cgcaagaccg
      781 cgccccgcac ccccgaggga gacatcgact acgaatctcc ccagtgcctg gatatggagc
      841 tgctggacca gcacttcgcc gccctgagcc ggggggagga gatcatcatc cccaaatttg
      901 agtttgcccg ccagatgcgc aacgacgccc ggggcacccc actgaagctg gggaagaatg
      961 agatcgccat ctttgagggc atccatgccc tgaacgacga catcgcaggt cctcacccgg
     1021 aggccgccaa gctgtatatc tccgcccgtt ccaatgtgaa cgacggggcg gccctccgat
     1081 tcaaggggac ctggatgcgg ctgacccggc gggcggtgcg ggattacagc ttccgcggca
     1141 caggtgtggc tgacacactg gagatgtggg ccaacgtgcg ccggggggag aagctgtata
     1201 tctcccccta taaaaaccgg gcggacatca tcttcgactc ctccctgccc tatgaggtgt
     1261 cggtgatgaa gaattttgcc ctgcctctgc tccaggcggt gccggaggag aacgcacggc
     1321 gtggcgagct gctggagctg atcgaggcgt tccagtggtt cgagcccatc gatccggagc
     1381 tggtgccggt ggactctctg ctgagggagt ttatcggcgg cggcagctat aagtaccatt
     1441 gaacgaagaa gagagagctg gggcggtagc ccgcgggctg tgtggcccgg ggcacgccgt
     1501 gccagaaaaa tgtggagtga tgtttgatgt ctgatcctct tgtctatgac tcaaggagcg
     1561 tgcggttcaa atccccctat ggagcggtcc cttccgggac ccaggtgacg ttcaccctcc
     1621 gccccctgcg gtcggagggc tactcccggg ggaagctcac cgcccggctg gagcagcggg
     1681 acaatcagat catcacggtg gagctgccct ggacagacac cgagctgggg cgggatgcct
     1741 tctccggcgt cctggacact ggggactatg tgggactggt gtggtacacc tttcagctgg
     1801 agaccatcac cggtcggcgc tggaacataa aggaggagta ccagcttacc gtctacgacg
     1861 ggagcgagat cgtgccccgc tggttcgggg agggggtgag ctaccagatc ttccccgacc
     1921 gcttccgccg cacccgggtg cctgaccccg cggggctggt aggggggcgc actgtccacc
     1981 agagctggca ggaggagccg gagtaccgac ccgacgccaa cggggagatc cgcaaccggg
     2041 acttcttcgg gggcgacctg aggggcgtga tagaggagct ggactacctc cagtccctgg
     2101 gggtagagac gctgtacttc aaccccatct tcgaagcggc ggagaaccac cgctacggca
     2161 cagctgatta cagccgggtg gacccaatgc tggggaccaa cgaggacttc tccgagctgt
     2221 gccggcaggc ccaccgccgg gggatgcggg tgatgctgga cggggtgttc aaccacaccg
     2281 gctttgtcag ccgctacttt aacggggacg gctattaccc ggagccaggg gcggtgcaga
     2341 gcgagagctc cccctaccgg ccctggttcc agttccggca ctggcctgac cagtacgaga
     2401 gctggtgggg gatctatacc ctgcctgctg tggaggagag ctgccccggc taccgggagt
     2461 tcatttttgg ggatgagaac tcggtggtcc gccgctggct gcgggcgggg gcggacggct
     2521 ggcgcctgga tgtggctgat gagctgcccg atgactttgt ggccgggatc cacaccgccg
     2581 cccgggcgga gaagcccggg gccctgctca tcggcgaggt gtgggaggac ggtaccacca
     2641 agatcgccta tggggtccgg agaaagcaca tcctgggccg ccactgcgac gggctgatga
     2701 actacccctt ccgaaatgcc gccctcaact ttctgcgggg cggggacggg gccaagttcg
     2761 tttccgccat ggaggccctg agggagaact acccctcttt tgccttttac tctgccatga
     2821 actctctggg cacccacgac accccccgca tcatcactct gctgggggtg ggcagcgagt
     2881 gcagggacca gagcaaggac tggcgggcct gcttccgtat gagtatggag cagtaccagc
     2941 ggggaaagga gctgctgaaa gtgggggcgg tactccttta cgcctttccc ggttccccca
     3001 cagtctatta cggcgatgag gcgggactgg agggctttga ggaccccttc aatcggagac
     3061 cctacccctg gggccgggag gatcgggagc tggtgggctg gttcgccgcc ctgggcaggc
     3121 tgcggaagga atgccccgcc ctgcggcggg gggatatccg gtatctggcc gggacgggcc
     3181 atctgctggc tttcctccgg agcacggagg gggagcgcct gctcaccgcg ctgaacgccg
     3241 gggacgagcc ggcggtcctg gctctgccct ggacggagga gccggagcgc ctgctgggcg
     3301 ctggggagtg ggccttgacc ggggagggcc cggtgctgat gctgcccccc cgcagcggcg
     3361 ggatctggcg cgtgggggag gcggagccgg cccagatgta gaagtgccgc cagtgggacg
     3421 aaaaagttca aacgccccct tgcatttccc tgaaaaacat gctatacttt tccctacgtc
     3481 cgggcataga cccgctgaac aaacgatttg cccgctctgg gctttgaaag gaacgagaaa
     3541 tgccatgaaa actgctgtta ccgtgatcca ggtgctgtgc ggactgtttc tgatcgccgt
     3601 ggtcctgctg cagtccggca agagcgcggg cctgtctggt gccatcgccg gcggcgcgga
     3661 taccttcctg tccaagaaca aggccaagac tgtggacgcc aagctggcca agtggaccaa
     3721 gtgggtggcc atcgccttta tgctgctcac cctgtccctg agcctgatcc cctgactgta
     3781 aaaagccccg cctgttttgt tttcaaaaca ggcggggctt t
//
LOCUS       DBMDSJ010000126         3819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6159_length_3819_cov_1.391608, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000126 DBMDSJ010000000
VERSION     DBMDSJ010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_3819_cov_1.391608"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(148..576)
                     /locus_tag="ACZPAQ_04170"
     CDS             complement(148..576)
                     /locus_tag="ACZPAQ_04170"
                     /inference="COORDINATES: protein motif:HMM:NF020464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HIR0903212.1"
                     /translation="MSKLVDMLMRPDTPNVQKDLPRADYELVRLSELYGEPFVLQLKA
                     VPYAKALELQQMEEYEVQTVLVGDDSGIWKDAELQADKGAATPAELVKLLLLPGEIRS
                     ISVAVEQLSGYRKPVLRQWDPKAKAPADPKRAVAEEIEKN"
     gene            complement(592..1020)
                     /locus_tag="ACZPAQ_04175"
     CDS             complement(592..1020)
                     /locus_tag="ACZPAQ_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR0903213.1"
                     /translation="MSSIDSAKRVMSGTFGELWWDGELIAEYYKFTAKYTQSKEAVNL
                     ARELIEDSKVMSTKGTGSMGIYKVYSRFKEYADQVQAGKDPRGTFVSKLDDPDAYGAE
                     RVALYNVSLDEVPLVNWERKTIQKDEVPFTFTRHKYMETT"
     gene            complement(1017..2117)
                     /locus_tag="ACZPAQ_04180"
     CDS             complement(1017..2117)
                     /locus_tag="ACZPAQ_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903214.1"
                     /translation="MADLGMPTLTITIKKAAETVVTRLKEGVVAMIVRDATATPGLYT
                     VGDVADIPDKLGANVAASVERALIGGINRPKKVLLVVIGAEDDLVQKGAAALAASDFN
                     YLSAPDDVDEDEKEALVTWLDGVRAKYCVGKLVLPDHAADNMAVVNFSASGIVAGGTT
                     YTGAQYCSRIAGLLAGTPLSNSATSSSLPEVTAVAEVANADAAVAAGKLILIHDGRKV
                     KLGRAVNSLTTVPPDQSEALKKIKIVDAVDLIRRESKILIEDQYVGQGNSYDNKLVLL
                     AAFQDFLTTLEKEGVLQEGSGYAELNLEKQRAWLKEQGVDVTDMTDQDILKADTGSFL
                     WIKLGGTILDGMEDFDLEFYMGGNTVTGGMAA"
     gene            complement(2110..2355)
                     /locus_tag="ACZPAQ_04185"
     CDS             complement(2110..2355)
                     /locus_tag="ACZPAQ_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903215.1"
                     /translation="MEALGDRLTLVLELFSCPAVQVGDRYLDLSAVHGDYFNDYAQVT
                     FTLSWQDDRETEQAEAALAKYYDLTIEVNKKEETHNG"
     gene            complement(2243..2581)
                     /locus_tag="ACZPAQ_04190"
     CDS             complement(2243..2581)
                     /locus_tag="ACZPAQ_04190"
                     /inference="COORDINATES: protein motif:HMM:NF044614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator family protein"
                     /protein_id="HIR0903216.1"
                     /translation="MLRINEILDAVEELMKERFPNSDAYRNLTPKDFKRPAFMVEMGK
                     QTMETFTRDLVERTAEVTDPVRGGRRLAQLPGGSSGRPAYPGAGAVFLPGGPGGRQIS
                     GPVCSPRGLF"
     gene            complement(2585..3040)
                     /locus_tag="ACZPAQ_04195"
     CDS             complement(2585..3040)
                     /locus_tag="ACZPAQ_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903217.1"
                     /translation="MQSFHLDDHDLSKLDERLAAAARRTPEIKREALQDVGEDLLAQV
                     QSKIGGSGKVRRWQHVHTGSGGGYVAVHAKEKETDKYGRAVGAVTNAIESGHRFPSPT
                     GKNKRYKFRNQSGRARVPGKHMYQSSSPEAAASAASARLGERLTQNLEE"
     gene            complement(3040..3603)
                     /locus_tag="ACZPAQ_04200"
     CDS             complement(3040..3603)
                     /locus_tag="ACZPAQ_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903218.1"
                     /translation="MSQVSIDAGRLDQRLEVLELKRVGDRWSWQPIRRTRANVTMSTR
                     SNLFSSVGIGARGAEIILRRQELTLHNALRWGDQHLFLTSILPEGRLHWKVSAALVEI
                     AQCQADMDEGGGARFPGVLTEKYMKFQQESPYSTNTITYVLVTPKAVTLQRGGVVAVN
                     GTDYAVQLAHVLDPYKNEYEICRTEDL"
     gene            complement(3600..>3819)
                     /locus_tag="ACZPAQ_04205"
     CDS             complement(3600..>3819)
                     /locus_tag="ACZPAQ_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903219.1"
                     /translation="KQPPASREDRHALWLSVACALTLDEYDQRGAQLESGKLQDNPSF
                     QRKLNQLKLTKPAANVSKLDTRGQEGVL"
BASE COUNT          611 a         1305 c         1080 g          823 t
ORIGIN      
        1 ggtcccggcg ctcctccagc tcatagagga ggaacgcccg gaccatggct ctctccccgg
       61 cggtcatgtt ccagtaccgt ccgggctccc accccttgat cacaaagagc aggtacagca
      121 tcaccaggtc gtcatccccg ccgtccctta gtttttttca atctcctccg cgacagcccg
      181 cttcgggtca gccggcgcct tggccttggg gtcccactgc cggagtaccg gcttgcggta
      241 gccgctcagc tgttccaccg ccacggaaat ggatcggatc tccccgggga gcagaaggag
      301 cttcaccagc tccgccggtg tggccgctcc cttatccgcc tgaagctcgg cgtccttcca
      361 aatcccgctg tcgtcgccaa ccaggaccgt ctggacctca tactcctcca tctgctgcag
      421 ctccagcgcc ttggcgtaag gaaccgcctt cagctggagc acaaagggct cgccgtacag
      481 ctcgctcagc cggaccagct cataatcggc cctggggagg tccttctgga cgttgggcgt
      541 gtccggacgc atcagcatat ctaccagttt gctcatatca aggctcctct cttatgtggt
      601 ctccatgtac ttgtgccggg tgaacgtgaa ggggacctcg tccttctgga tggtcttgcg
      661 ctcccagttg accaggggca cctcgtccag ggacacgttg tacagcgcca cccgctcggc
      721 gccgtaggcg tccggatcat ccagcttgct gacaaaggtg ccgcgggggt ccttgccggc
      781 ctgcacctga tcggcgtact ctttgaagcg gctgtagacc ttgtagatcc ccatggagcc
      841 ggtgcccttg gtgctcatca ccttggagtc ctcgatcagc tcccgggcca ggttgaccgc
      901 ctccttgctc tgggtgtact tcgcggtaaa cttgtagtac tcagcgatca gctcgccgtc
      961 ccaccacagt tccccgaagg tccccgacat cactctcttt gcgctgtcaa tggaactcat
     1021 gccgccatac ctcctgtcac ggtgttgccg cccatgtaaa actcaaggtc aaagtcctcc
     1081 atgccgtcca ggatcgtgcc gcccagcttg atccacagga aggagccggt atcggccttc
     1141 aggatgtcct ggtccgtcat atcggtcacg tccacgccct gctccttcag ccaggcccgc
     1201 tgcttctcca gattcagctc ggcatagccg ctgccctcct gcaggacgcc ttctttctcc
     1261 agggtggtca ggaagtcctg gaaggccgcc agcaggacca gcttgttgtc gtagctgttg
     1321 ccctggccca catactggtc ctcgatcagg atcttggact cccggcggat caggtccacc
     1381 gcgtccacga tcttgatttt cttcagggcc tcgctctgat ctgggggaac tgtggtcaga
     1441 ctgttcaccg cccgccccag cttcactttg cgcccatcgt ggatcaaaat cagcttcccg
     1501 gcggccacgg cggcgtcagc gttggccacc tcggccactg ccgtcacttc gggcaaagac
     1561 gaggaggtag cggaattgct caggggcgtt ccggccagca ggccggcgat ccggctgcag
     1621 tactgggcgc cggtgtaggt ggtgccgccg gccacgatgc cggaggcgct gaagttgacc
     1681 accgccatgt tatccgcagc gtgatccggc aggaccagct tgcccacgca gtatttggcc
     1741 cggaccccat ccagccaggt gaccagcgcc tctttctcat cctcgtccac atcgtccggg
     1801 gcggacaggt aattgaaatc gctggcggcc agggcagcgg cgcccttctg caccagatca
     1861 tcttcggcgc cgatcaccac cagcaggacc ttcttggggc ggttgatgcc gccgatcagc
     1921 gcccgctcca cactggcggc tacatttgcc cccagcttgt ccgggatgtc cgccacgtct
     1981 cccacggtgt agagtccggg ggtagccgtg gcgtcccgca caatcatggc cacaaccccc
     2041 tccttcagcc gggtcactac cgtttccgcg gccttcttga tggtgatggt cagggtaggc
     2101 atacccaaat cagccattgt gtgtctcctc cttcttgttg acctcaatgg tgaggtcgta
     2161 gtattttgcc agggccgcct ctgcctgctc cgtctcacgg tcgtcctgcc aggacagggt
     2221 aaaggtcacc tgggcgtaat cgttaaaata gtccccgtgg actgcagaca ggtccagata
     2281 tctgtcgccc acctggaccg ccgggcagga aaacagctcc agcaccaggg taagccggtc
     2341 tcccagagct tccacctggg agttgtgcca gacgtctacc tcctcgaaca gggtcagtca
     2401 cctcggccgt ccgttccacc agatcccggg taaaggtctc catggtctgc tttcccatct
     2461 ccaccatgaa agcgggccgc ttaaagtcct tgggcgtcag gttgcggtaa gcgtcgctgt
     2521 tcgggaaccg ctccttcatc agctcctcca ccgcgtccag tatctcgttg attctcagca
     2581 caggtcattc ctccaggttt tgtgtcagcc tctcgcccag acgggcggaa gcggcggagg
     2641 ccgcggcctc cggactgctc gattggtaca tatgcttgcc gggcactctg gccctcccgc
     2701 tttgattgcg gaatttatac cgtttgttct tccctgtcgg gctggggaac ctgtggccgg
     2761 attcaatcgc gttggtcacg gcccccacag cccggccata tttgtccgtt tctttctcct
     2821 tcgcgtggac cgccacataa ccgccgccgg agccggtgtg gacatgctgc catctccgaa
     2881 ccttccccga gccgccgatc ttgctctgca cctgggccag aagatcctcg cccacatcct
     2941 gcagggcctc ccgcttgatc tccggggtcc tgcgggcggc ggccgccagg cgctcgtcca
     3001 gtttggacag gtcatggtcg tcaagatgaa aggactgcat cacagatcct ccgtccggca
     3061 gatctcgtac tcgttcttgt acgggtccag cacatgggcc agctgtacgg cgtagtcggt
     3121 cccgttgacg gccaccacgc cgccccgctg gagcgtcaca gccttgggcg tcaccagcac
     3181 ataggtgatg gtgttggtgg aatacgggct ttcctgctgg aatttcatgt acttctccgt
     3241 caggacgccc ggaaacctcg cgccgccgcc ctcgtccatg tccgcctggc attgagcgat
     3301 ctccaccagc gcggccgaca ctttccagtg cagccggccc tctggcagga tggaagtgag
     3361 gaacaggtgc tggtcccccc agcgcagggc attgtgcaga gttagttcct gccggcggag
     3421 gatgatctcc gcccctcggg cgccgatccc caccgagctg aagagattgc tccgggtact
     3481 catcgtcaca ttggcccgcg tcctccggat gggctgccag ctccaccggt ctccgacgcg
     3541 cttcagctcc agcacctcca gccgttggtc cagcctgccg gcatcaatgc ttacctggct
     3601 catagaactc cctcctggcc cctagtgtcc aacttggaca catttgccgc cggcttcgtc
     3661 agcttcagct ggttgagctt tctctggaag gatggattgt cctgtagctt gccgctctcc
     3721 agctgggcgc cccgctggtc atactcgtcc agggtcaggg cgcaggccac actgagccag
     3781 agggcgtgcc ggtcctcgcg ggaggccggc ggctgcttc
//
LOCUS       DBMDSJ010000127         3802 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6190_length_3802_cov_1.560389, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000127 DBMDSJ010000000
VERSION     DBMDSJ010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_3802_cov_1.560389"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="ACZPAQ_04210"
     CDS             complement(<1..1722)
                     /locus_tag="ACZPAQ_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024589.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HIR0903220.1"
                     /translation="MLNVTMLQTPSVTLDGVPVSFPFKRADALLYYMLVRRSATRQEL
                     ISLLWESCDEATGLKNLRNTLYTLKKTLGGDFLLSPQKSLVVVNSAWEVDCDYDRFTQ
                     QGDFSAYRGPFLQGFAVKHAFSFDEWLDRTREKLHEQYLGRLAQQAQAALEAGDPEEA
                     ARLATEYLREDPFDESMAAFLMERLRAARKYSRAAQVYQRLKEQLSEELGVDPLESTT
                     MLYYEIMNQWNDTTQAPGEAEAPQVPVGRETLYAALRAAAGSFAEGAVRRCSQLFIGE
                     VGSSKSELISHFLRWSDLSALLVLRCGCLQSEEGLPLAPWDRILLSLREFIQEEQLSL
                     PVPVQVRLGQAFPLFRSENAWPGGVSARAMRQWDQGLEDSLLLLFSAVTRRKKVLLVL
                     EDLQWADEESLRLGDALLRRLEGRGLMTILTARDDLSPSALARLESLEADGLLRRHRL
                     PPLTPEQTEELLRRELGGEAARRMAPQFHRETGGNLYLLTELTQAYRRSGDVEATLQT
                     LGDILMDRLSGLSGAALQVAELISLFSEEVPSRLLLELMDQNDRLLTAGLDELRGRGI
                     ILEHHTEG"
     gene            1912..2811
                     /gene="ftcD"
                     /locus_tag="ACZPAQ_04215"
     CDS             1912..2811
                     /gene="ftcD"
                     /locus_tag="ACZPAQ_04215"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - L-histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR0903221.1"
                     /translation="MKLDKWIESVPNFSEGRDLKKVERIVDSFRAKPGVKLLDYSTDP
                     DHNRCVVTVLGQPEPLKRAMVEAIGTAVELIDMTRHEGQHPRIGCVDVIPFIPVRDCT
                     IQEADQLAREIAQEAALLYGQPFYLYEKSATAPHRVDLANIRKGQFEGLAEKMKDPLW
                     KPDFGPDTIHPTGGATAIGARMPLICFNINLDTPNVEIARQIARRVRNIGGGLHYVKA
                     MGIALEERNLAQVTMNLTDYTKSSIYSVFEMVKMEARRYGVRVVGSEVVGIVPMKALL
                     DCAEYYLQIEDFSSEQVLELHIE"
     gene            complement(2875..>3802)
                     /locus_tag="ACZPAQ_04220"
     CDS             complement(2875..>3802)
                     /locus_tag="ACZPAQ_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4317 domain-containing protein"
                     /protein_id="HIR0903222.1"
                     /translation="LADITFSTQQVVDSEEHRLLMALRGSSLADEEAMQALFQRVISS
                     LTLEGNYLILLAHDTYDVPYRAKDGERMDDASDQVFSYILCSICPVKLTKPALSYFVY
                     ENAFHDLKADWVVSPPEAGFLFPAFDERTTNLYNALYYSRDIGENHPELIDALFRTPP
                     PMPAAVQKETFESLLGETLAEDCSYHVVQAVHSQLRDMIEEHKASKEEQPLLLSKGEV
                     RSVLSSCGVAAPHVDAFEAQYDQAFGPEAGLSPRNLVDPKQLEVRTPDVTIHVNPERS
                     DLVETRMIEGTRYILIRANEGVEVNGVSIQIS"
BASE COUNT          681 a         1164 c         1338 g          619 t
ORIGIN      
        1 cccctcggtg tggtgctcca ggatgatgcc ccggccccgc agctcgtcca gaccggcggt
       61 gagcaggcgg tcattctggt ccatcagctc cagcagcagg cggctgggca cctcctcgga
      121 gaagagggag atgagctcgg ccacctggag ggccgcgccg gacaggccgg agagccggtc
      181 catcaggata tcccccaggg tctggagggt ggcctccaca tcgccgctgc ggcggtaggc
      241 ctgggtcagc tcggtgagca gatagaggtt gcctccggtc tcccggtgaa actggggagc
      301 catgcgccgg gcggcctcac cgcccagctc cctgcggagc agctcctctg tctgttccgg
      361 ggtgagaggg ggcaggcgat ggcggcggag caggccgtcc gcctccagcg actccagccg
      421 ggccagggca gagggggaga ggtcgtcccg ggcggtgagg atggtcatca gccccctgcc
      481 ctccaggcgg cggagcaggg cgtcccccag acgcaggctc tcctcgtcag cccattgcag
      541 gtcctccagc accaggagca cctttttccg gcgggtgaca gcggagaaga ggagcaggag
      601 gctgtcctcc agcccctggt cccactggcg catcgcccgg gcggagactc cgcccggcca
      661 ggcgttctca gagcggaaga gagggaacgc ctggcctaat cggacctgga ccgggaccgg
      721 gagggagagc tgctcctcct ggatgaactc ccgcagggac agcaggatgc ggtcccaggg
      781 ggccagaggg aggccctcct ccgactggag gcagccgcac cgcagcacca gcagggcgga
      841 cagatcggac cagcggagga agtggctgat gagctcgctc ttgctggagc ccacctcccc
      901 gatgaacagc tgggagcagc ggcgcaccgc cccctcggcg aaggagcccg ccgccgcccg
      961 cagggcggca tagagggtct cccgtcccac ggggacctgg ggcgcctctg cctccccggg
     1021 ggcctgggtg gtgtcgttcc actggttcat gatctcgtaa tagagcatgg tggtgctctc
     1081 cagggggtcc acacccagct cctcggagag ctgctccttc agccgctggt acacctgggc
     1141 ggcccgggag tacttccggg ccgcccgcag gcgctccatg aggaaggcgg ccatggactc
     1201 gtcaaagggg tcctcccgca gatactcggt ggccaagcgg gcagcctcct ccgggtcgcc
     1261 ggcctccagg gcggcctggg cctgctgggc cagccggccc agatactgct catggagctt
     1321 ctcccgggtg cggtccagcc actcgtcgaa ggagaaggcg tgcttgacgg cgaagccctg
     1381 gaggaagggg cctcggtagg cggagaaatc cccctgctgg gtgaagcggt cgtagtcgca
     1441 gtccacctcc caggcgctgt tcaccaccac cagggacttc tggggggaga ggagaaagtc
     1501 gccccccagg gtctttttca gcgtatataa ggtgttgcgg agatttttca ggccggtggc
     1561 ctcgtcgcag ctctcccaca gcagagagat gagctcctgc cgggtggcgc tgcggcgcac
     1621 cagcatgtag tacagcaggg cgtccgcccg cttgaaggga aaggagaccg ggaccccgtc
     1681 cagcgtgacg ctgggggtct ggagcatggt cacattgagc atcagaaagc ccccctcttt
     1741 ggtctgggtc cagtatatcg gatttggggc cgggtgtgca actgttcccg ggcaaaaatt
     1801 tgccgggggc gcgtttttgc cttgcctggg ggaggggaaa acactataat aatataaaaa
     1861 ggtaaaagag gggggcgtcc ccgtggccca ggacagagag aggagagaga catgaagctg
     1921 gataaatgga tcgaatccgt ccccaatttc agcgaggggc gggacctgaa gaaggtggag
     1981 cgcatcgtgg actccttccg ggccaagccg ggagtgaagc tgctggacta ctccacagac
     2041 ccagaccaca accgctgcgt ggtgacggtg ctgggccagc cggagcccct gaagcgggcc
     2101 atggtggagg ccatcggcac ggcggtggag ctgatcgata tgacccggca cgaggggcag
     2161 cacccccgca tcggctgcgt ggacgtgatc cccttcatcc cggtgcggga ctgcaccatc
     2221 caggaggcgg accagctggc ccgggagata gcacaggaag ccgctctgct ctacggacag
     2281 cccttctacc tctatgagaa gtctgccact gccccccacc gggtggacct ggccaacatc
     2341 cgcaagggac agttcgaggg gctggcggag aagatgaagg accccctgtg gaagccggac
     2401 ttcgggccgg ataccatcca ccccactggc ggggccaccg ccatcggggc ccggatgcct
     2461 ctgatctgct tcaacatcaa cctggacacc cccaatgtgg agatcgcccg gcagatcgcc
     2521 cgccgggtgc ggaacatcgg cggcggcctg cactatgtga aggccatggg catcgccctg
     2581 gaggagcgga acctggctca ggtgaccatg aacctgaccg actacaccaa aagttccatt
     2641 tacagtgtct ttgagatggt gaagatggag gcccggcgct atggcgtccg ggtggtgggc
     2701 agcgaggtgg tgggcattgt ccccatgaag gccctgctgg actgcgcgga gtactacctc
     2761 cagatcgagg acttctcctc cgagcaggtg ctggagctgc atatcgaata gacggacaag
     2821 caaaaggagc ggcccctctg gaacagaggg gccgctctcc ctttttcgac agtttcagga
     2881 gatctggatg ctgacgccgt tgacctccac gccctcgttg gcccggatga ggatgtaccg
     2941 ggtgccctcg atcatgcggg tctccaccag gtcgctgcgc tccgggttga cgtggatggt
     3001 gacgtcggga gtgcggacct ccagctgctt ggggtccacc agattgcggg ggctcaggcc
     3061 ggcctccggg ccgaaggcct ggtcatactg ggcctcaaag gcatccacat ggggggcggc
     3121 cacgccgcag gaggacagga cggaccgcac ctcccccttg gagagcagga ggggctgctc
     3181 ctccttgctg gccttgtgct cttcgatcat gtcccgcagc tggctgtgga cggcctggac
     3241 cacgtgatag ctgcaatcct ccgccagcgt ctcccccagg agggattcaa aggtctcctt
     3301 ctggacggcg gcaggcatgg gaggaggggt gcggaacagg gcgtcaatga gctccgggtg
     3361 gttctccccg atgtcccggg agtagtagag ggcattatag aggttggtgg tgcgctcgtc
     3421 aaaggcgggg aagaggaagc ctgcctccgg cggagagacg acccagtccg ccttcaggtc
     3481 gtggaaggca ttttcataga caaagtagct cagggcgggc ttggtcagct tcaccgggca
     3541 gatggagcac aggatgtagg agaacacctg gtcggaggcg tcgtccatcc gctccccgtc
     3601 tttggcccgg taggggacgt cgtaggtgtc gtgggccagg aggatgagat agttcccctc
     3661 cagggtgagg gaggagatga cccgctggaa cagggcctgc atggcctctt cgtccgccag
     3721 ggaggagccc cgcagggcca tgaggagccg gtgctcctcg ctgtccacca cctgctgggt
     3781 ggagaaggtg atgtccgcca gg
//
LOCUS       DBMDSJ010000128         3801 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6192_length_3801_cov_1.593784, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000128 DBMDSJ010000000
VERSION     DBMDSJ010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6192_length_3801_cov_1.593784"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="ACZPAQ_04225"
     CDS             complement(<1..1394)
                     /locus_tag="ACZPAQ_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit domain-containing
                     protein"
                     /protein_id="HIR0903223.1"
                     /translation="MEERDEESGRFVCDFVERLPTTDTGKPFRLYEWQRETLMEFYST
                     MERDEESGRLLRKYQYLYLEIPKKNGKSELSAALGLYHLFGDGELNAEVYLCAADKDN
                     AGIVFRAAVFMLETAPWTAKMIARGELKVVRSQKRIEYRRRVKAENGGLRWVTVGLMQ
                     VLSSESYSKHGYKPSCVIFDELHAQPDRKLWDVMTGAAGAAHDQPVWIVLTTAGDDPD
                     RHSIGWEIHERAVAIRDARQLRRILAEGGDPRQVLSLRRAEEDDLPRAQAALLERDES
                     NWLPVLYGLTAMFGDDPDDLDRVDIWDEALWYRCNPSLGKHLTLRALRLEAQAAKKSP
                     AAEKLFRWLRLNQWISVKAVGWLPLTLYDKTQWNRPEWKVLSAPERRAAVREYLRGKK
                     CYGGLDLSTTTDLTALALLFPPQAGLESWVVLFWAWRPEDGTLEAEQRDHVPYRDWAR
                     AGFLELCPGDMVDFS"
     gene            complement(1398..1904)
                     /locus_tag="ACZPAQ_04230"
     CDS             complement(1398..1904)
                     /locus_tag="ACZPAQ_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HIR0903224.1"
                     /translation="MPGPRQPTDLVKARGKKHFSQTEEDQRRDQEVHVQPPDRAEPPR
                     WLGKKFHAEFREIGEILRQAGLYTELDRDVLGQFLVARERWLRSDRLASAAIRARDEK
                     LAKEWTAVQSSYFRQCRQCAEVMGLSISSRCRLVVPEVLANAAKDEGEEDEFTRALKA
                     RQARAAGG"
     gene            complement(1925..2677)
                     /locus_tag="ACZPAQ_04235"
     CDS             complement(1925..2677)
                     /locus_tag="ACZPAQ_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR0903225.1"
                     /translation="MDRETIKRQIQLYHGDCLVGMERVESGSVDLILCDLPYGTTDCS
                     WDTVIPFEPLWAQYRRVLKPRGAAVLMAAQPFATDLINSARKLFRYDLVWEKSAPVGF
                     ANCHRQPLRSHELILVFYRHLPTYHPQGLIKLEKPIQKAAARRGGNVYHSLGRACVQT
                     YTNYPRSILRFPFRGRRENPTQKPLELMEYLVNTYTDPGDLVMDNCMGSGTTAVAALN
                     TGRRFIGFERELNYYQTACRRVADRAEELTAA"
     gene            complement(2867..3229)
                     /locus_tag="ACZPAQ_04240"
     CDS             complement(2867..3229)
                     /locus_tag="ACZPAQ_04240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR0903226.1"
                     /translation="MALKPLRPCRHPGCAALTREGYCPKHKPQKAPRRVSAEYHSWYS
                     LPIWTDDLRPAQLLREPFCRACAKRGDRIRATVVDHIKPHRGSWPLFIDPANHQSLCK
                     RCHDRKTALEQRKTGGFF"
     gene            complement(3300..3488)
                     /locus_tag="ACZPAQ_04245"
     CDS             complement(3300..3488)
                     /locus_tag="ACZPAQ_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025568.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HIR0903227.1"
                     /translation="MRCGKIIVRDGWVICPVCQKGKLLKVRADTTARNLPRKCKRCGQ
                     ETLVNIEAPEPASKVTSA"
     gene            complement(3574..>3801)
                     /locus_tag="ACZPAQ_04250"
     CDS             complement(3574..>3801)
                     /locus_tag="ACZPAQ_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rolling circle replication-associated protein"
                     /protein_id="HIR0903228.1"
                     /translation="ERTWTPSLGLARTEPETETVPDYVTLTAPPEAIILQNQGPVRNG
                     YGEFAWIKYMLPYQPDRRRARSKRRRRRKKE"
BASE COUNT          606 a         1355 c         1106 g          734 t
ORIGIN      
        1 gagaagtcca ccatatcccc ggggcacagc tccaggaagc ccgcccgggc ccagtcccgg
       61 tatgggacgt gatcccgctg ctccgcctcc agggtcccgt cctccggccg ccaggcccaa
      121 aacaggacca cccagctctc cagccccgcc tggggcggga agagaagggc cagggccgtc
      181 aagtcggtgg tggtggacag gtccagcccg ccgtagcact ttttccccct cagatactcc
      241 cgtaccgccg cccgccgctc cggcgcgctg agcaccttcc actccggccg gttccactgg
      301 gtcttgtcgt acagggtcag gggcagccag cccaccgcct tgacgctgat ccactgattg
      361 agccgcagcc atcggaacag cttttccgcc gccgggctct ttttggctgc ctgggcctcc
      421 agccgaagcg cccgcagcgt cagatgcttc cccagggacg ggttgcaccg gtaccacagc
      481 gcctcgtccc agatgtccac ccggtccagg tcgtccgggt cgtccccaaa catggcagtc
      541 aggccgtaca gcaccggcag ccagttgctc tcgtcccgct ccagcagggc cgcctgggcc
      601 cgggggaggt cgtcctcctc cgccctccgc agggagagca cctgtcgggg gtcacccccc
      661 tccgccagga tgcggcgcag ctgccgggcg tcccggatgg ccaccgcccg ctcgtggatc
      721 tcccagccga tgctgtgccg gtccgggtcg tcgcccgcag tggtcagcac gatccaaacc
      781 ggctggtcgt gggcggctcc ggccgcgccg gtcatcacgt cccacagctt ccggtcaggc
      841 tgggcgtgga gctcatcgaa gatcacgcag ctgggcttgt agccgtgctt ggagtaggac
      901 tccgaggaga gcacctgcat cagccctacc gtgacccagc gcaggccgcc attctccgcc
      961 ttcacccgcc ttcggtattc gatccgcttt tgggatcgga cgaccttcaa ctctcctcgg
     1021 gcgatcattt tggcggtcca gggggcggtc tccagcatga acaccgccgc ccggaacacg
     1081 atgcccgcat tgtccttatc cgcggcgcag agatacacct ccgcgttcag ctccccgtca
     1141 ccgaacaggt gatacaggcc cagggcggcg gacagctcgc tctttccgtt cttcttgggg
     1201 atctccagat agagatattg atacttcctc agcagccggc cgctttcctc gtcccgctcc
     1261 atggtggagt agaactccat cagcgtctcc cgctgccact catacagccg gaagggcttc
     1321 cccgtgtccg tagtgggcag gcgctccaca aagtcgcaca caaagcgccc gctctcttcg
     1381 tcccgctcct ccatggctca gccccccgcc gccctggcct gcctggcctt cagcgcgcgg
     1441 gtgaactcgt cttcctcgcc ctcgtccttg gccgcgttgg ccagcacctc cggcaccacc
     1501 agccggcacc gggaggagat ggacagcccc atcacctcgg cgcactgccg gcactgccgg
     1561 aaataggagc tctggacagc cgtccactcc ttagccagct tctcgtccct ggcccggatg
     1621 gcggcggagg ccagcctgtc cgaccggagc caccgctccc gggctaccag gaactggccc
     1681 agcacatccc ggtccagctc cgtgtaaagg ccggcctggc gcaggatctc cccgatctcc
     1741 cggaactcag cgtggaactt cttccccagc caccggggcg gctccgcccg gtccggcggc
     1801 tgcacatgga cctcctggtc cctgcgctgg tcctcctccg tctgggaaaa atgctttttc
     1861 cccctggcct tcaccagatc cgtgggctgt ctcggtcccg gcatcccgca tcacctccct
     1921 gctcctatgc cgccgtcagt tcttccgccc ggtccgccac ccgccggcag gccgtttggt
     1981 agtaattcag ttcccgctca aagccgatga acctccgccc ggtattcagc gccgccacag
     2041 ctgtggtccc gctccccatg cagttgtcca tcaccaggtc tcccgggtcc gtgtaagtgt
     2101 tcaccagata ctccatcagc tccagcggct tctgcgtggg attctcccgg cgcccccgga
     2161 aggggaagcg aaggatcgag cgggggtagt tggtgtaggt ctgcacacat gcccgcccca
     2221 ggctgtgata gacatttcct ccccggcggg cagccgcctt ctggattggc ttctccagct
     2281 tgatcagccc ctgggggtga taggtcggaa ggtgcctgta aaataccagt atcagctcat
     2341 gagaccgcag gggctgcctg tggcagttgg caaaacccac tggagccgat ttctcccaga
     2401 ccagatcata gcggaacagt tttctggcgc tgttgatcag gtccgtcgca aagggttggg
     2461 ccgccatcag caccgcggcc cctctcggct tcagcacccg gcggtactgc gcccacagcg
     2521 gttcaaacgg gatcacagta tcccaggagc agtctgtagt gccatacggc aggtcgcaga
     2581 ggatcagatc cacgcttccg ctctccaccc gctccatgcc caccaggcag tcgccgtggt
     2641 agagctgtat ctgccgcttg atcgtttctc gatccatcac tttcgcctcc catctcatgg
     2701 ggaaattttt tcgcgcagcg ggatgcctgc ggtcttgcac actttcacgc agactttttt
     2761 gacccggggg gagggtctga ggccgaaggc ctccctgcgc ccggataggc gcatgcccag
     2821 ccgcacgccc gcgcccaagc gtcccgccat gtctcccgtg cagtgctcaa aaaaagcccc
     2881 ccgttttccg ctgctccaag gccgtcttgc ggtcatggca gcgcttgcac aggctctggt
     2941 ggttggccgg gtcgatgaac agcggccagc tcccccggtg cggcttgatg tggtccacta
     3001 ccgtggcccg gatacggtca ccacgcttgg cgcacgcgcg gcagaacggc tcccgcagga
     3061 gctgcgccgg gcgtaggtcg tccgtccaga taggcaggct gtaccagctg tgatattccg
     3121 ccgagacccg gcgaggggct ttctggggct tgtgcttggg acagtacccc tcccgggtca
     3181 gggcggcaca ccccgggtgt cggcacggcc tgagcggctt cagggccatg ggctatcacc
     3241 tccaggcaaa acaaaaagcg cccaagccac gacacccgac tgctcgggtt gtcatcggct
     3301 caggcgctgg tcactttgga cgcgggctca ggcgcttcga tattcacgag ggtctcctgt
     3361 ccgcagcgtt tgcacttgcg gggcaggttt cgggcggtcg tgtctgcgcg gaccttcagg
     3421 agtttcccct tttggcacac cgggcagatc acccatccat ccctcacgat gattttacca
     3481 caacgcatgt tcgattgcaa atctttttgc cccctttttc tgagaaatca aagagaatat
     3541 acttaccccc gagaccgaaa agaatagaaa agcctattct ttcttccgtc tgcgccgccg
     3601 cttgctcctg gcccgtctcc ggtccggctg gtagggcagc atatatttga tccaggcaaa
     3661 ttcgccgtat ccattgcgga caggtccctg attttgcagg ataatagcct cgggtggagc
     3721 cgtcagcgtc acatagtccg ggacggtctc cgtctccggc tctgtccggg ccagccccag
     3781 ggagggggtc caggtccgct c
//
LOCUS       DBMDSJ010000129         3771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6244_length_3771_cov_1.721526, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000129 DBMDSJ010000000
VERSION     DBMDSJ010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6244_length_3771_cov_1.721526"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="ACZPAQ_04255"
     CDS             complement(<1..268)
                     /locus_tag="ACZPAQ_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HIR0903229.1"
                     /translation="MSIILIHPYPGRPEADVRLSGILSHALADREGRTIRTAEELDLR
                     PGDRVLFALALDGAGQNLEYYRMLSRLRREPDLLEGCTAALIVDG"
     gene            complement(314..718)
                     /locus_tag="ACZPAQ_04260"
     CDS             complement(314..718)
                     /locus_tag="ACZPAQ_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903230.1"
                     /translation="MREKILAHRIRWLIGLCTFLVLFGCSGFPIYNFTTGRQLGSWYF
                     MLMLCIGYFEIGQLLGVLYSRNKVWRSAALALGMTLLGLACRFLMEFGEVSNTCNFTP
                     PNVALHLSVAAVITALGSLSFVDGRRPPLGKD"
     gene            complement(802..2040)
                     /locus_tag="ACZPAQ_04265"
     CDS             complement(802..2040)
                     /locus_tag="ACZPAQ_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HIR0903231.1"
                     /translation="MFYYYEKAGKLLASERRDLPYLKAEPAPGTPICYLVEGDPVLGR
                     GSFKVNCPGQLRAPHGLEVLDASRLPDFPLDAPLSAALEEGLLTAVNTGRAGWEQVLS
                     QRAGTEKKRVNILAIGDVGSTLLTGLKLLGGDVISSIGICDLSDQITARWEFEMGQIS
                     LPWDYGAFPEVEVISLEKLFDCDVFVFVASRGIPPVGSQVKDVRMAQFENNAAIVKQY
                     ARMARAADFQGLWCAVSDPVDPLAKTAYLESNRDENGVWDGKGLRPEQIQGFGLGVMN
                     ARAAYFAKRDPRLASFLTEGRSFGPHGTGLFIANSIDHYDEEVSRELTHRTVTANLVM
                     REIGFKPYVAPALSSGALSILLTLRGAWHCGSVFLDGVFMGVKNRYTPAGVETELLPR
                     IPDPLFGHIREAAEHLKSVL"
     gene            complement(2095..>3771)
                     /locus_tag="ACZPAQ_04270"
     CDS             complement(2095..>3771)
                     /locus_tag="ACZPAQ_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase-related domain protein"
                     /protein_id="HIR0903232.1"
                     /translation="PEGKELGITGKVIQLQLPPHLEDISSTRIRENVDMNRDISTFID
                     PVIQDFIYQNGLYLRDTQDKPLLNARDLSFQWVERPDPLLVADVTAGHRERESLRTAI
                     LRDGDQLLVLRHRREGGEPMLGWLSFRYLSTADLYSAFQNADLADQVRLRAGGKTLFI
                     TGLAAEEEQEKDYAQLLVSEVLSHALEAECVYAVFHPHEGLCPPETEELFRRQGFIRR
                     DGGPLEADLRAPVVLLQNLETTIQEPLSRNPRVLAAIRRGHQRLETALTGLYPGNLVL
                     TLSADVIHHRLLRMITQFNQVPQEPTTPRKLGKYMCVPFGKLLRGKTVPNTVTKTIHT
                     DRVYSPDLSESTIEAFPNYASIPCQVRTIKSFDRPVILVDDLMHPGVRIKALDPVLRQ
                     EGVDIQLVLVGILSGYGRDLMSQQKRPVDSVYFLPSLRQWFVEATLYPFIGGNTVRRS
                     RPPVPGLLPGINHILPYAVPPFAKECGEEAVFQLSRCCLESARDVIGTLEREYRALYG
                     RNLTLGRLAEAVILPLCPDKGTCLGYDPNLAASVYLENDLEQLLRTHPRG"
BASE COUNT          712 a         1195 c         1224 g          640 t
ORIGIN      
        1 ggccatccac gatcagcgcg gcggtgcatc cctccagcag gtccggctcc cgccgcagcc
       61 gggagagcat gcggtaatac tccaaattct gtcccgcccc gtccagggcc agagcaaaca
      121 ggacccggtc tcccggcctc aggtccagct cctccgcggt gcggatggtc ctcccctccc
      181 ggtccgccag ggcgtgggag aggataccgg acaggcggac gtccgcctcc ggccggcccg
      241 ggtatggatg gatcaaaatg atggacatga gatcgccttc ttcaaaagtt gatgtgcgga
      301 gagaccggaa agttcagtct tttcccaggg gtggccgccg accgtccaca aaggacaggg
      361 accccagggc ggtgatcacc gcagcgacag agaggtggag cgccacattg gggggcgtaa
      421 agttgcaggt attggacacc tcaccaaact ccatcaaaaa ccggcaggcc agccccaaaa
      481 gcgtcatacc gagggcgagg gcagctgatc tccacacctt gttccggcta tacagcacac
      541 ccaggagctg tccgatttca aagtaaccga tacagagcat tagcataaaa taccagctgc
      601 caagctgccg ccctgtggtg aaattataga tggggaagcc agaacagccg aaaagtacca
      661 gaaatgtaca gagaccgata agccagcgga tgcggtgggc caaaattttt tctctcatgg
      721 gacaccccct ttgtcatgca gggcaagtta ttccattcgg atgcaggggc acaacaaatc
      781 gcgtccttac cggttttccc tctataatac cgactttaaa tgctccgccg cctcccggat
      841 atggccgaac agggggtccg ggatacgggg cagcagctcg gtctccaccc cggcaggggt
      901 atagcggttc ttgaccccca taaagacccc gtccaggaag acggagccgc agtgccacgc
      961 cccccgcagg gtaagcagga tggacagggc tccggaggac agcgcagggg ctacataggg
     1021 cttaaagccg atctcccgca tgaccagatt ggcggtgacc gtcctgtggg tcagctcccg
     1081 ggagacctcc tcgtcgtagt ggtcgatgga gttggcgatg aacagtcccg tgccgtgggg
     1141 gccgaaagag cgcccctccg tcaggaagga ggccagcctg gggtcccgct ttgcaaagta
     1201 ggccgcccgg gcgttcatca cccccaggcc gaagccctgg atctgctctg gccgcaggcc
     1261 ctttccatcc catacgccgt tctcgtcccg gttgctctcc agataggcgg tcttggccag
     1321 ggggtccacc gggtcggaca ccgcgcacca cagcccctgg aagtcggccg cccgtgccat
     1381 gcgggcgtac tgcttcacaa tggcggcgtt gttttcgaac tgggccatgc gcacgtcctt
     1441 cacctgggag cccaccggcg ggatgccccg ggaggccaca aagacgaaca catcgcagtc
     1501 aaacagcttt tccagggaaa tgacctccac ctccggaaat gccccgtagt cccagggcag
     1561 gctgatctgc cccatctcga actcccaccg ggcggtgatc tggtcggaga ggtcgcagat
     1621 gccgatggat gagatcacat ccccgcccag cagcttcagc ccggtgagca gggtggagcc
     1681 cacgtccccg atggccagga tattgacccg ctttttctcc gtcccagccc tctgggacaa
     1741 cacctgctcc caccccgccc ggccggtatt gacggcagtg agcagcccct cctccagggc
     1801 ggcggacagg ggcgcgtcca ggggaaagtc cggcaggcgg gaggcgtcca gcacctccag
     1861 gccgtggggg gccctcagct ggcccggaca gttcaccttg aaggagcccc ggcccagcac
     1921 cgggtccccc tccaccagat agcagatcgg agtccccggg gcgggctccg ccttgagata
     1981 ggggagatcc ctccgctcag aggccaggag ctttccagcc ttttcgtaat aatagaacat
     2041 aatattacac ctcgcaatgg tttcccgcag atcatttcca gcagtcggcg gaggtcagcc
     2101 ccgggggtgg gtcctcagca gctgctccag gtcgttctcc agatagaccg acgcggccaa
     2161 gttggggtca taacccaggc aggtgccctt gtcgggacac aggggcagga tgaccgcctc
     2221 agccagccgg cccagagtca gattccggcc gtagagtgcc cggtactccc gctccagggt
     2281 gccgatcaca tcccgggcgg actccaggca gcagcgggag agttggaaga cggcctcctc
     2341 cccgcactct ttggcgaagg gagggacggc atagggcagg atgtggttga ttcccggcag
     2401 caggccgggg actgggggcc gggagcggcg cactgtgttg cccccgatga aggggtacag
     2461 ggtggcctcc acgaaccact gccgcagaga gggcaggaag tacacgctgt ctaccggccg
     2521 cttctgctga ctcatcaggt cccggccgta gccggagagg atgcccacca ggacaagctg
     2581 gatgtccacc ccctcctggc gcaggacggg gtccagggcc ttgatgcgca ccccggggtg
     2641 catcaggtcg tccaccagga tgacgggccg gtcaaaggat ttgatggtgc gcacctggca
     2701 ggggatggag gcgtagttgg gaaatgcctc gatggtgctc tcgctgaggt caggggagta
     2761 cacccggtcg gtgtggatgg tcttggtgac cgtgttgggc acagtcttgc cccggagcag
     2821 cttaccgaag gggacgcaca tatatttccc cagctttcgg ggggtggtgg gctcctgggg
     2881 cacctggttg aactgggtga tcatccgcag cagccggtgg tggatcacgt cggcagacag
     2941 ggtgaggacc aggttgcctg gatacaggcc ggtgagggcg gtctcgagcc gctggtgtcc
     3001 ccgccggatg gcggcgagca ccctggggtt gcggctcagg ggctcctgaa tggtggtctc
     3061 caaattctgg agcagcacca ccggggcccg cagatcggcc tccagcggcc ccccatcccg
     3121 gcggatgaag ccctgccgcc ggaagagctc ctctgtctcc ggggggcaca gcccctcgtg
     3181 ggggtggaag acagcgtaga cgcactccgc ctccagggcg tgggagagca cctcactgac
     3241 cagcagctgg gcatagtcct tctcctgctc ctcttcggcg gccaggccgg tgatgaacaa
     3301 agttttacct cccgcccgca ggcgcacctg gtccgccaga tcggcgtttt gaaacgcgga
     3361 gtagaggtcg gctgtagaca gatagcggaa gctgagccaa cccagcatgg gctccccgcc
     3421 ctcccgacgg tggcgcagca ccagcagctg gtccccgtcc cggaggatag cggtccgcag
     3481 gctctcccgc tcccggtgcc cagcagtgac atcggccacc agcagggggt ccggccgctc
     3541 cacccactgg aaggacaggt cccgggcgtt gagcaggggc ttgtcctggg tgtcgcggag
     3601 gtacaggccg ttctgataga tgaagtcctg gatcaccggg tcgatgaagg tggagatgtc
     3661 ccggttcatg tccacgttct cccggatacg ggtagagctg atgtcctcca ggtggggggg
     3721 cagctggagc tggatcactt ttccggtgat gcccagctcc tttccctccg g
//
LOCUS       DBMDSJ010000130         3758 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6271_length_3758_cov_1.463768, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000130 DBMDSJ010000000
VERSION     DBMDSJ010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6271_length_3758_cov_1.463768"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="ACZPAQ_04275"
     CDS             <1..1877
                     /locus_tag="ACZPAQ_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903233.1"
                     /translation="DSNNVESIAQYTQIMTELKGADWAKENIRLTGYPSEIYAYWGES
                     DHSTTRINGWYFDDAAFYGPDLEIVDGQIVYNTKLSDGDTYTLDCRGSAAGSSKQGYK
                     YTVYDELYQEWALDKKGASVTAGAPAADQITSIQATTVANFYGYKVGTVDITYAAGTD
                     LTGVTADSYTLYDRGFNNPEFGEVEITGASVDGNVVTLTVDQGTDKVTDRSRETYGTL
                     CTSSSWYVDSEGNIHYGDTQTTDALGITIYPNTIKKGLQWRENMDLVLCVNSDDITTG
                     IRSTDGAGTMLADTVWEPTILADDLDKVELEMVDVGWKAADYTMLGDQGEVPVHVIYP
                     DNYDPNRAEPYPVIDYQCGGGVCYWEVTDGSTTPANNLGCNVVYDVMMTEWHRQMPDA
                     IIMSVNVHSSTVENSAKEIAGVLDYAIENWNADKDRIILVGNSQGTLISSDVIRQRPD
                     LVAGYVECNGNLGGMASADAVDGTLANSSLGSWTEEEVQAMIDNEVSVWMFNGETDGD
                     NPAAQQDVIEIVKDLYREAGKSESWIEEHVRASGLQSWKFKDWGETDHSVTKVVAWYY
                     LGQPYMDVEKGTSLKAGDTYQYSGKEESYEKYQYTMDYVYTVYPESVAQWAKDVFASV
                     "
     gene            2071..2772
                     /locus_tag="ACZPAQ_04280"
     CDS             2071..2772
                     /locus_tag="ACZPAQ_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HIR0903234.1"
                     /translation="MKREKQMGEAPAVLWREEKKTLTLCGEPVLEETLAWPEFEGKGR
                     AVRRMTGYYRRLVRSCRLRWERETYCLACLRLAACREESRVFRPWQAGLTGEVAMEGA
                     DLLSIRMELREVRGDGRPLLSCSGVTWDWKAGAPVPLGMLLPERSRRRQLLEGIRQAG
                     EGELRSGSCLLDPDWKETARRKFRASRFCLRPEGIELFYPQCSLAPAVEGVPSFRVPL
                     AGAAWTAGEGTASET"
     gene            2782..>3758
                     /locus_tag="ACZPAQ_04285"
     CDS             2782..>3758
                     /locus_tag="ACZPAQ_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR0903235.1"
                     /translation="MAAYLLFEVMVMLLSRLNDVVWSPWLLGLFLAAGLYYSLRTGFF
                     QLFRIPLWLRTTFGAMLRRRGRGNGRGISQFQALATALASTIGTGSIAGVATAIWFGG
                     PGAVFWMWVSALLGMMTGCAEKLLSVRYRRAGPAGWLGGPMYYIRDGLKSPLLAGWFA
                     LACLPATLTGGDMVQSSSIAAALQATFGWDRLAVGAAVAVLTGLVMVGGIGRIARVSS
                     GLVPAMALLYLGGGAAVLAARADQIPEAMALIFCSALRPEAAVGGGMGYTLAAALRYG
                     VARGVFTNEAGLGTSAMAHAAAEVDCPARQGMWGIVEVCVSTLLVCTPPR"
BASE COUNT          679 a         1105 c         1313 g          661 t
ORIGIN      
        1 aggactccaa caacgtggag agcatcgctc agtacaccca gatcatgacc gaactcaagg
       61 gggccgactg ggcgaaggag aacattcgtc ttaccggtta tccctctgag atctatgcct
      121 actggggtga gagcgaccac tccaccaccc gtatcaacgg ctggtacttt gatgatgccg
      181 ctttctatgg ccccgacttg gaaatcgtgg acggccagat cgtctacaat accaagctga
      241 gcgacgggga tacttatacc ctggactgcc ggggcagcgc cgccggcagt agcaagcagg
      301 gctataagta caccgtgtac gacgaactgt atcaggagtg ggctctggac aagaaaggcg
      361 cctccgtcac tgccggagct cccgccgctg accagatcac ctctatccag gccacaaccg
      421 tggctaactt ctatggctac aaggtgggta cagtggacat cacctacgcc gcgggtactg
      481 acctgaccgg cgtcaccgcc gacagctaca ccctgtatga ccggggcttc aataatccgg
      541 agttcggcga ggtggagatc accggcgcct ctgtggatgg gaacgtggtc accctgaccg
      601 tcgatcaggg gacggataag gtcaccgacc gcagccgtga gacttacgga actctgtgca
      661 ccagctccag ctggtatgtg gattcagagg ggaacatcca ctatggcgat actcagacca
      721 ctgatgccct gggtatcacc atctacccca acaccattaa aaagggcctg cagtggagag
      781 agaatatgga cctggtgctg tgcgtcaaca gcgacgacat caccaccggt atccgctcca
      841 ctgacggcgc tggcactatg ctcgccgata ccgtctggga gcccaccatc ctggccgatg
      901 acctggacaa ggtggagctg gagatggtgg atgtaggctg gaaagctgca gactacacta
      961 tgctgggcga tcagggcgag gtccccgtcc atgttatcta ccccgacaac tatgacccca
     1021 accgggcgga gccctacccc gtcatcgact atcagtgcgg cggcggcgtc tgctactggg
     1081 aggtcactga cggcagcacc accccggcca acaacctggg ctgcaacgtg gtctatgacg
     1141 tgatgatgac cgagtggcac cgccagatgc ctgacgccat cattatgagt gtcaacgtcc
     1201 actcctccac agtagagaat tctgccaagg agatcgccgg cgtgctggat tacgccatcg
     1261 agaactggaa cgcggacaag gaccgcatca tcctggtggg caactcccag ggcaccctga
     1321 tctcctccga cgtgatccgc cagcgccccg atctggtggc gggctatgtg gagtgcaacg
     1381 gcaacctggg cggcatggca tccgctgatg cggtggacgg caccctggca aacagcagcc
     1441 tggggagctg gaccgaggaa gaggtccagg ccatgatcga caacgaggtg tccgtgtgga
     1501 tgttcaacgg cgagaccgat ggtgacaacc ccgctgccca gcaggacgtg atcgagatcg
     1561 tgaaggacct gtaccgggaa gccgggaaga gcgagtcctg gatcgaggag catgtccggg
     1621 cctccgggct ccagtcctgg aagttcaagg attggggcga gaccgaccac tccgttacca
     1681 aggtggtggc ttggtactat ttgggccagc cctacatgga tgtggagaag ggcacctctc
     1741 tgaaggccgg cgacacctac cagtattccg gcaaggagga gagctacgag aagtatcagt
     1801 acaccatgga ctatgtgtac acggtgtacc cggagagcgt ggcccagtgg gcgaaggacg
     1861 ttttcgcctc tgtgtaagcg cggatagagc cggtcccgca ggccggcgga aggaagagca
     1921 ttactttgct tcctctctca cgctctgcgg agcgtctccc taaaagagcg ccgggcagac
     1981 ccgtaagggc ctgcccggcg ctcttttagg gaaagggacc gttttttctg ccccgtcata
     2041 ggatagggaa agaaatgggg gcggtttggc atgaaaaggg aaaaacagat gggggaggcc
     2101 ccagcggtcc tgtggcggga ggagaaaaag accctgactc tgtgcgggga gccggtgctg
     2161 gaggagaccc tggcctggcc ggagtttgag gggaaggggc gggctgtgcg ccgcatgacg
     2221 ggctactacc ggcgcctggt ccggtcctgc cgcctgcgct gggagcggga gacctactgc
     2281 ctggcctgcc tgcggctggc cgcctgccgg gaggagtccc gggtcttccg gccctggcag
     2341 gcagggctga cgggagaggt agccatggag ggggcggacc tgctgagcat ccggatggag
     2401 ctgcgggagg tgcggggaga cgggcggccc ctcctgtcct gttccggggt gacctgggac
     2461 tggaaagcgg gcgcgccggt tccgctgggt atgctgctgc cggagcggag ccgccggcgg
     2521 cagctgctgg aggggatcag gcaggcaggg gagggggagc tccggagtgg gagctgtctc
     2581 ctggacccgg actggaagga gacggcgagg cggaaatttc gggccagccg cttctgcctg
     2641 cgcccggagg gcatcgagct cttttatccc cagtgttctc tggccccggc ggtagagggg
     2701 gtgccctcct tccgggtgcc cctggcgggg gctgcgtgga cggcggggga agggacagcc
     2761 tcagagacat aaagctgggg catggccgca tacctcttat ttgaggtgat ggtcatgctg
     2821 ctctcccgtc tgaatgacgt cgtctggtcc ccctggctgc tggggctgtt cctggcagcg
     2881 gggctgtatt actccctgcg gaccggcttt ttccagctgt tccgcatacc cctgtggctc
     2941 cgcaccacct tcggagccat gctgcgccgg cgggggcggg ggaatgggcg gggtatctcc
     3001 cagttccagg ccctggccac cgccctggct tccaccatcg gtacaggcag catcgccggg
     3061 gtggctaccg ccatctggtt cggcgggccg ggggcggtgt tctggatgtg ggtctccgcc
     3121 ctgctgggca tgatgacagg gtgcgcggag aagctgctct ccgtgcgcta ccgccgcgcc
     3181 ggccccgcag ggtggctggg agggcccatg tactacatcc gggacggcct gaaaagcccg
     3241 ctgctggcgg gctggtttgc cctggcatgc ctgccggcca ccctgaccgg gggagacatg
     3301 gtccagtcca gctccattgc cgccgccctc caggccacct tcggatggga ccggctggcg
     3361 gtgggggcgg ccgtcgcggt cctgacgggg ctggtgatgg tgggggggat cggacgcatc
     3421 gcccgggtga gcagcggtct ggtcccggcg atggcgctgc tctatctggg gggcggggcg
     3481 gcggtgctgg ctgcccgggc cgaccagatc ccggaggcga tggcactgat cttctgctcg
     3541 gcgctccgac cggaggccgc agtagggggc ggaatgggat acaccctggc tgccgctctg
     3601 cggtacgggg tggcccgggg ggtgttcacc aatgaggcgg ggctggggac ttccgccatg
     3661 gcccacgctg cggcagaggt ggactgtcca gcccggcagg ggatgtgggg gatcgtggag
     3721 gtgtgcgtct ccaccctgct ggtatgcaca cctccacg
//
LOCUS       DBMDSJ010000131         3752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6279_length_3752_cov_1.442618, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000131 DBMDSJ010000000
VERSION     DBMDSJ010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6279_length_3752_cov_1.442618"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /gene="yabQ"
                     /locus_tag="ACZPAQ_04290"
     CDS             complement(<1..220)
                     /gene="yabQ"
                     /locus_tag="ACZPAQ_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HIR0903236.1"
                     /translation="MTISVAQQAAALAQAAALGLAVGLLYDLFRVLRVRVALPLLGAV
                     LDLSFWIIVTVALFVWSQQAWRGEIRLYG"
     gene            complement(222..509)
                     /gene="yabP"
                     /locus_tag="ACZPAQ_04295"
     CDS             complement(222..509)
                     /gene="yabP"
                     /locus_tag="ACZPAQ_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HIR0903237.1"
                     /translation="MAYEENMPRMEGAHHVILEGREQLSVSGVEEVESFDENTIIMTT
                     VKGTLVVRGEDLHIEKLSLDGGDLRVEGVVDSLSYEDDSRERGGFFARLLR"
     gene            824..1624
                     /locus_tag="ACZPAQ_04300"
     CDS             824..1624
                     /locus_tag="ACZPAQ_04300"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR0903238.1"
                     /translation="MREGELSGHLAGCLVGSRLLCLDTIDSTNTEAKRQAMAGAREGL
                     VVLAEEQTGGRGRLGRSFQSPRGCGLYLTALLRPQLPPIEVVDFTAWVAVAVCDGIQA
                     ACGVRPRIKWTNDIVLEGKKLCGILTEMGLENEINALQYLVTGIGVNVNQRPEDFSPD
                     VRPIAASLFNVLGRPVRRSELAVQIIRALDRMYSAFPQGKADYLARYRADCLTPGNRV
                     QLITPASRTEATALSIDDEFRLVVEYDDGRREALSAGEVSVRGMYGYV"
     gene            complement(1674..3110)
                     /locus_tag="ACZPAQ_04305"
     CDS             complement(1674..3110)
                     /locus_tag="ACZPAQ_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR0903239.1"
                     /translation="MRGPALLAALSMVLTAVPAALAVETAAGTPPAVTEAPYSDVPAE
                     AWYAPFAADLKQDGAVSGYEDGTFRPDNAVTVGEALVMVLAAAGFDRREATGEHWSSG
                     NADFALEKGFVTREQVTDLDSAVSRLTVAQMAAKALNLRPSRNETPFGDVDDGYVTVL
                     YEKIQLSGELEEDGTLSYHPEDSITRSELVAIIWRMKNTDVHAGQIAYEGYSQTFYLD
                     VLETVPAFSYIPQLFRMEGDYLTYDDGTVQGHLGVDVSQFQGEIDWEQAADAGVEFAI
                     VRVGGRGYTEGKLYEDTYFDRNVQGALDAGLAVGAYFFSQAITVEEALEEARFVLERV
                     RDYDLTMPVVFDWEMVSDSSARTNAIDSDTMNEVALAFCGAIQEEGYESMIYFNSYQG
                     YVKYDLSKLLDHSFWFAQYQAQPSFYYDFQMWQYSSKGSVPGIQGEVDMNLYFGALEG
                     GERPKLPNGQEAPLDPGINPETGRPYGY"
     gene            3370..>3752
                     /locus_tag="ACZPAQ_04310"
     CDS             3370..>3752
                     /locus_tag="ACZPAQ_04310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR0903240.1"
                     /translation="MWKDCQALQDEITALRRELHQIPEVGAQLPETAACLSAHLDRLG
                     IPFVRSPRDSGLIASIQGGLPGRTVALRADMDALPIQEETGLPFSSRRPGVMHACGHD
                     AHAAMLMGAAQVLQKHRKELRGTVRL"
BASE COUNT          647 a         1321 c         1101 g          683 t
ORIGIN      
        1 tcccgtacag ccggatctcc ccccgccatg cctgctgaga ccagacgaac agcgccactg
       61 tcacaatgat ccagaaggac agatccagca ccgcccccag caggggcagg gccacccgga
      121 cccggaggac acggaacagg tcatacagca gccccaccgc cagccccagc gccgccgcct
      181 gggccagggc ggcggcctgc tgcgccaccg aaatggtcat gtcagcgcag cagacgggcg
      241 aagaatccgc cccgctcccg gctgtcgtcc tcataggata gggaatccac cacgccctcc
      301 acccgcaggt ctcccccgtc cagagagagc ttctcgatgt gcagatcctc cccccggacc
      361 accagggtcc ccttcactgt ggtcatgatg atggtgtttt catcgaagct ctccacctcc
      421 tccacgccgg agacggacag ctgttcccgc ccctccagaa tgacatggtg tgccccctcc
      481 atcctgggca tgttctcttc atacgccatt gcgttcaccc tttctcgttt ttcccattta
      541 tatgggacag gacctggggt tatgcctgtc tgccagcctc agttgacacc acccagcccc
      601 cacgggtata ataaagacca caagttctga tcgccgattt aaaggagggc cctgccttga
      661 gccgagaaac cgttctctcc ctgctgctgg cgcacccggg ggaatatctg tccggggcgg
      721 ccatgagccg cacactgggt gtcagccgcg ccgccgtgtg gaaggccatc gaggctctcc
      781 gccaggaggg atatgagacc gcctggagtc cgcccccgac cgggtgcggg agggggagct
      841 gtccggccac ctggccggct gtctggtggg gagccgcctg ctctgtctgg acaccatcga
      901 ctccaccaac acggaggcca agcgccaggc tatggcgggc gcgcgggagg gcctggtggt
      961 gctggcggag gagcagaccg gcggccgggg ccggctgggc cgctccttcc agtccccccg
     1021 gggctgcggg ctgtatctga ccgcccttct ccgcccccag ctccccccca tagaggtggt
     1081 ggacttcact gcctgggtgg cagtggctgt atgcgacggc atccaggccg cctgcggcgt
     1141 ccgccccagg atcaagtgga ccaacgacat cgtgctggaa gggaaaaagc tgtgcggtat
     1201 tctcaccgag atggggctgg agaacgagat caacgccctc cagtatctgg tcaccggcat
     1261 tggcgtgaat gtcaaccagc gccccgagga cttcagcccc gacgtgcggc ccatcgcagc
     1321 ctccctcttc aatgtcctgg gccgcccggt gcgccgctcc gagctggcgg tccagatcat
     1381 ccgggctctg gaccggatgt actcagcctt tccccagggc aaggctgact atctggcccg
     1441 gtaccgggcc gactgcctca cgccgggcaa tcgggtacag ctcatcaccc ccgcctcccg
     1501 caccgaggcc accgccctgt ccatcgacga cgagttccgt ctggtggtgg agtacgatga
     1561 cggccgccgg gaggccctct ccgcgggaga ggtgtccgtc cgcgggatgt acggatatgt
     1621 gtaacaaaat cagtcaagcg gtccgccccg tagggcggac cgctcgcttt ctctcaatat
     1681 ccgtagggcc ggccggtctc cgggttgatg cccgggtcca gcggggcctc ctggccgttg
     1741 ggcagtttgg ggcgctctcc gccctccagg gctccgaaat agaggttcat gtccacctcg
     1801 ccctggatac ccggcacact ccccttggag ctgtactgcc acatctggaa gtcatagtag
     1861 aaactgggct gcgcctggta ctgggcgaac cagaagctgt gatccagcag cttgctcagg
     1921 tcgtacttca catacccctg ataggagttg aagtagatca tagactcata gccctcctcc
     1981 tggatggcgc cgcagaaagc cagtgccacc tcattcatgg tgtcgctgtc gatggcgttg
     2041 gtgcgggcgc tgctgtcgct gaccatctcc cagtcaaaga ccaccggcat ggtcaagtca
     2101 tagtctcgga cccgctccag gacgaaccgc gcctcctcca gcgcctcctc cacggtaatg
     2161 gcctgggaga agaagtaggc ccccaccgcc agcccggcat ccagcgcgcc ctggacgttc
     2221 cggtcaaagt atgtatcctc atagagcttg ccctcggtgt agccccggcc gcccacccgg
     2281 acgatagcga actccacacc ggcatcggcc gcctgctccc agtcgatctc cccctggaac
     2341 tgggacacat ccaccccaag gtgcccctga accgtcccat catcataggt gaggtagtct
     2401 ccctccatgc ggaacagctg ggggatgtag gagaaggcgg ggactgtctc cagcacatcc
     2461 aggtagaagg tctggctgta cccctcatag gcgatctgcc ccgcgtggac atcggtgttc
     2521 ttcatccgcc agatgatggc gaccagctcg gagcgggtga tgctgtcctc cggatggtaa
     2581 gacagcgtgc catcctcctc cagctccccg gagagctgta tcttttcata gagcacggtg
     2641 acatagccat cgtccacgtc tccgaagggc gtctcgttcc gggagggcct caagttcagg
     2701 gctttggccg ccatctgggc caccgtcagc cggcttaccg cactgtccag atcggtcacc
     2761 tgctcccggg tgacaaagcc cttctccagg gcaaagtccg cattgccgct gctccagtgc
     2821 tccccggtgg cctcccggcg gtcaaagcct gccgccgcca gcaccatcac cagggcctcc
     2881 cccaccgtaa cggcgttgtc cggccggaag gtgccgtcct catagccgct caccgccccg
     2941 tcctgtttca ggtccgccgc gaagggggcg taccacgcct ccgccggcac atccgaatag
     3001 ggggcctccg tgactgcggg cggggtcccg gccgctgtct cgaccgccag ggccgcgggc
     3061 accgctgtca gcaccatgga cagagccgcc agcagagccg gtcccctcat gtttcttctc
     3121 ttctgctgca atgggatcat cctttcctgc ccggcgccgc gggcccgtga tatgttgaca
     3181 taataccgca ttttttgacc cattgcaacc atatcccacc ttcgaagaag agaaattttt
     3241 tcctgctgat ctccctgggc accgtccccc ttgcggtcct gcccggcgcc gcttataata
     3301 gggacatgtg ccggcaggca gtcctgccgg aaattttaga tcttcacacc tggaaggagc
     3361 ccgtctaaca tgtggaagga ttgtcaagcc ctgcaagatg agatcactgc cctgcgccgg
     3421 gagctgcacc agatcccgga ggtgggggca cagctgcccg agaccgccgc ctgtctctcc
     3481 gcccatctgg accgcctggg tatccccttc gtccgctctc cccgggacag cggcctgatt
     3541 gcctccatcc agggcggcct gcctggtcga acggtggccc tgcgggcaga catggacgcc
     3601 ctgcccatcc aagaggagac cggacttcct ttttcctccc gccgccccgg cgtgatgcac
     3661 gcctgcgggc atgatgccca cgccgccatg ctcatggggg ccgcccaagt cctgcagaaa
     3721 cacaggaagg agctccgggg caccgtccgc ct
//
LOCUS       DBMDSJ010000132         3742 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6302_length_3742_cov_1.584729, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000132 DBMDSJ010000000
VERSION     DBMDSJ010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6302_length_3742_cov_1.584729"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="ACZPAQ_04315"
     CDS             complement(<1..588)
                     /locus_tag="ACZPAQ_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR0903241.1"
                     /translation="MAFVEFEQRGHVGLITINRPEVLNALNLEVIEQLDDILLRAESQ
                     EDVHVIVITGAGRSFVAGADIGEMVNYTADDAKRFSHHGNNTMMHITRFPSPVIAAVN
                     GFALGGGCELAMSCDIRVASEKATFGMPEVGLGITPGFGGTQRLQRIVGMSTAMELVL
                     TSRTIDAQEALQIGLVKHVYPADVMLDKALELADLI"
     gene            879..2414
                     /locus_tag="ACZPAQ_04320"
     CDS             879..2414
                     /locus_tag="ACZPAQ_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR0903242.1"
                     /translation="MLRHDEWSAGSSALFLTALAAVSQGLSFCYRVALSRLVGAEVMG
                     LYQLLMPVCSVLLSLTAVGLTAAMSNLSSQYLALGNGKGVAQTRRMCLTALGVGLLPV
                     SAAVVAFYDPISVYLLGDARTQLGLILLLPCVALTGVENIHKHFFYGTGVVRPPAVVE
                     LMEQFIRAAAVLGLLVLFLPQNPERTVGLIVTGMVICEIFSAVALTVLYRRRMARIGR
                     TGPGERGGTLARRVTSIAVPVGATALLGNLMSAVNAALIPQKLVESGMDRAAAMSEFG
                     VVCGMTLPMLALPTVFLGALNLVLVPRLAYSTALNRPEEVRRRAGRAMLAVSVLILPS
                     MALMVVLGPDLARMMFGQPSAGEHLLPLAAAMALSCYQSTLGGVLNGVGRPGSHAMAD
                     LICDGIQLAFTFTVGLPGVGIRGFVAGTLVSAAAGALLNGWLVVRYTGLRPSLFRWVT
                     APGLAALLAALTSNLLFRWLKDSGVSLLAGGAASLLFGGVLYLAALSAQGVYLSQVFR
                     LRK"
     gene            complement(2457..3017)
                     /locus_tag="ACZPAQ_04325"
     CDS             complement(2457..3017)
                     /locus_tag="ACZPAQ_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR0903243.1"
                     /translation="MRKNFGPKPYLYPQPVLIIATYGDDGTPDAMNAAWGGISDSNRV
                     AMYLSAGHKTVQNILSRKAFTVSMADEDHVVECDYVGIVSANQAPDKLARAGLHTTRS
                     QFVDAPLIDELPMALECQFLSFDEETELLLGEIINVSAEEYILGEDGKIDPAKLRPIS
                     FDPVNHDYLALGEKVGRAFQDGKRLK"
     gene            complement(3168..3548)
                     /gene="rplL"
                     /locus_tag="ACZPAQ_04330"
     CDS             complement(3168..3548)
                     /gene="rplL"
                     /locus_tag="ACZPAQ_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR0903244.1"
                     /translation="MASEKITALIEEVKGLTVLELSELVHALEDEFGVSAAAMAAPAA
                     GAGAAAPAAEEKTEFDVVLASFDAAAKIKVIKAVREITGLGLAEAKAMVEGAPKTLKE
                     AVSKDEAEELKKKLEEAGAKVELK"
BASE COUNT          625 a         1108 c         1248 g          761 t
ORIGIN      
        1 gatcaggtcg gccagctcca gagccttgtc cagcatcacg tcggcagggt agacatgctt
       61 gaccaaaccg atctgaaggg cctcttgggc gtcgatggtg cggctggtga gcaccagctc
      121 catggcggtg gacatgccta cgatgcgctg aaggcgctgg gtgccgccga aaccgggagt
      181 gatgcccagc ccaacctcag gcatgccgaa ggttgccttt tcactggcta cccgaatgtc
      241 gcaggacatg gccagctcac agccgccgcc cagggcgaag ccgttgactg cggcgatcac
      301 cgggctgggg aagcgggtga tgtgcatcat ggtattgttt ccatgatggc tgaagcgctt
      361 ggcgtcgtcg gcggtatagt tgaccatctc gccgatgtcg gcaccggcca cgaaggagcg
      421 tccggcgccg gtgataacga tgacgtggac atcctcctgg gattctgcgc gcagcaggat
      481 atcgtccagc tgttcgatga cctccaggtt cagggcgttc aggacttccg ggcggttgat
      541 ggtgatgagg cccacgtggc cgcgttgctc aaattctaca aaagccatag gggtatcctc
      601 ctttttacag tgtctcattc ttcttccaga aaatcgcggg gataaagggg acgggatctc
      661 tttcttaatc gtactataaa cgatttcccg ggcggaagca aggcgtctgg tgacaaaggt
      721 aaagatacgc cagtttttgg tcaattcagg catgattctc ccctggaacg agagaaatat
      781 gtgaaaaaga taacgaattt cttgcaggaa tggcggggcg gcggcgtgaa tacagtgaag
      841 ggaggaccgc ccgctggggc ggggaggagg gactggattt gctgcgtcac gatgaatggt
      901 ccgctggaag cagcgccctg tttctgaccg ccttggcggc agtatcacag gggctgtcgt
      961 tctgttaccg ggtggccctg tcccggctgg tgggggcgga ggtgatgggg ctgtatcagc
     1021 tgctgatgcc ggtgtgctcg gtactgctgt ccctcaccgc tgtggggctg accgcagcca
     1081 tgtccaacct cagctcccag tatctggccc tgggaaacgg aaagggagtg gcgcagacca
     1141 ggcggatgtg cctcaccgcc ctgggagtgg ggctcctgcc ggtatcggcg gcagtggtgg
     1201 ccttctatga ccccatctcg gtctatctgc tgggggatgc ccggacccag ctggggctga
     1261 tcctgctgct gccctgcgtg gctctcaccg gggtggagaa catccacaag cactttttct
     1321 atgggaccgg agtggtccgc ccgcctgctg tcgtggagct gatggagcag ttcatccggg
     1381 cggcggcggt gctggggctg ctggtgctgt ttctgcccca gaacccagag cggacggtgg
     1441 ggctgatcgt caccggcatg gtgatttgtg agatcttttc cgccgtggcg ctgacagtgc
     1501 tctaccgccg gcggatggcg cggatcggcc ggacggggcc gggggagcgg ggcgggactc
     1561 tggcccggcg ggtgacctcc atcgccgtcc ctgtgggggc caccgccctg ctgggtaatc
     1621 tgatgtcggc ggtgaacgcc gcccttatcc cacagaagct tgtggagagc ggcatggacc
     1681 gggcggcggc catgtcggag ttcggcgtgg tgtgcgggat gaccctcccc atgctggccc
     1741 tgcccactgt ctttctgggg gcgctgaatc tggtgctggt ccccaggctg gcctactcca
     1801 cggcactgaa ccgcccggaa gaggtgaggc ggcgggccgg gcgggccatg ctggcggtgt
     1861 cggtgctcat tcttccctcc atggcgctga tggtggtgct tgggccggac ctggcccgga
     1921 tgatgttcgg ccagccctcg gcgggggaac acctgctccc cctggcggcg gccatggcgc
     1981 tgagctgcta ccagtccacc cttgggggcg tgctcaacgg cgtgggacgg ccggggagcc
     2041 acgccatggc ggacctgatc tgtgacggca tacagctggc cttcaccttc accgtgggcc
     2101 tcccgggggt gggcatccgc ggcttcgtgg cggggactct ggtctcagcg gcggcggggg
     2161 ccctgctcaa cggctggctg gtggtgcgtt acaccgggct gcggcccagc ctgttccggt
     2221 gggtcaccgc gccggggctg gccgcactgc tggcggccct gacctccaac ctcctctttc
     2281 ggtggctgaa ggacagcggc gtctccctgc tggcaggcgg ggcggccagc ctactcttcg
     2341 gcggagtgct gtatctggcc gccctgagcg cccagggagt ctatctcagc caggtgttcc
     2401 ggctgcggaa atagggcggc cgctccagaa agggccccaa acccctcccg gagagctcac
     2461 tttagccttt ttccatcctg gaaagcgcgg cccaccttct ctcccagggc cagatagtcg
     2521 tgattgaccg ggtcgaagga gatggggcgc agcttggcgg gatcgatctt cccatcttcc
     2581 cccaggatgt actcctcggc gctgacgttg atgatctccc ccaggagcag ctccgtctcc
     2641 tcgtcaaagc tgaggaactg gcactccagc gccattggca gttcgtcaat gaggggggcg
     2701 tccacaaatt ggcttctggt ggtgtggagg ccggctcggg ccagcttgtc aggcgcctgg
     2761 ttggcggaca cgatacccac atagtcacac tccaccacat ggtcctcgtc cgccatgctt
     2821 acagtgaaag cctttcggga cagaatgttc tgtacggtct tgtgtcccgc gcttagatac
     2881 atagcgacac ggttgctgtc gctgatcccg ccccaggcgg cgttcatggc atctggggtc
     2941 ccgtcatctc cataggtggc gatgatcagc accggctggg gatagaggta gggcttgggg
     3001 ccaaagtttt tccgcatgat ggattcctcc cattatatta agatcgggga tgctgtgatg
     3061 gtactaggat aacacgaaac agcggcggag gacagaggga gaaaaagaaa aaacgggccc
     3121 cggggcattt tgccctgggg cccgttgact tctgtctggg gaaaagctta cttcagctcg
     3181 accttggcgc cggcctcttc cagcttcttc ttcagctcct cggcctcatc cttggagaca
     3241 gcctccttca gagtcttggg agcgccctcg accatagcct tggcctcagc caggcccagg
     3301 ccggtgatct cacggacagc cttgatgacc ttgatcttag cggcggcgtc aaagctggcc
     3361 aggaccacgt caaactcggt cttctcctca gcagcgggag cggcagcgcc ggcaccagca
     3421 gcgggagcgg ccatagcggc ggcggaaacg ccgaactcgt cctccagggc atgcaccagc
     3481 tcggacagct ccagaacggt caggcccttg acttcctcaa tcagagcggt aatcttctcg
     3541 ctagccataa tgaatgtacc tcctaaaagt tgttttcagt caaaatgtta tgggtgccga
     3601 tacttactcg gcgggggcgg cctcgggctc ggcagcggcc tctacgaagc cgcccttctc
     3661 ctcggcaatg gccttcagga cgatggccag gctggtgatg ggggccagca tggtgcccag
     3721 gacctgggcc tgcaggacct cc
//
LOCUS       DBMDSJ010000133         3740 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6305_length_3740_cov_2.056334, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000133 DBMDSJ010000000
VERSION     DBMDSJ010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_3740_cov_2.056334"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="ACZPAQ_04335"
     CDS             <1..486
                     /locus_tag="ACZPAQ_04335"
                     /inference="COORDINATES: protein motif:HMM:NF019128.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR0903245.1"
                     /translation="RIALPLEVLAEAAGLQLTWDEVEGAAWLSTDQAQPASASYPAED
                     LYWLSRIISAESRGEPLLGQIAVGNVILNRVESSQYPDTVEGVVFDTKYGVQFQPVSN
                     GTIYDAPASSSLVAAKLCLEGTDVVGESLYFFSPALSAGRWIVSNATYYTTIGGHQFY
                     V"
     gene            complement(615..2273)
                     /locus_tag="ACZPAQ_04340"
     CDS             complement(615..2273)
                     /locus_tag="ACZPAQ_04340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR0903246.1"
                     /translation="MAEKLKIISLGGLNEIGKNLTVYEYGGDIVVVDCGMGFPDDDMY
                     GIDVVIPDVSYLIKNQNKVRGIFITHGHEDHIGALPYVLRSLNAPIYATRMSAGLIRL
                     KLEEHRLLDKTKIITCEAGDVIKAGKFTVEFIHVNHSIADAVAFAIKCPVGTCVHTGD
                     FKIDATPIQGGMIDLARLGQLGNEGVLALLADSTNVERPGYTRSERSVGNSFDALFKG
                     CEERIIVTTFASNVDRLQQIIDVAARYGRRVAVTGRSMENAMKVSTELGYMNIPDGVL
                     MDLNQIKSLPKNRVCIITTGSQGETMSALSRMAFSTHRQVDILPGDRIIISASAIPGN
                     EHSIGNVINELYRKGAEVLNERELALHVSGHACQEELKIIHALVKPKFFIPVHGEQRM
                     LQTHAKLAREMGMDSNHILISDIGRVMELTQNSARLAGTVTAGQVFVDGLGVGDVGSV
                     VLRDRRHLAQDGMIVVVMSMSGEDGSLVSGPDLITRGFVYVKESEGLMEELRQVALEA
                     INSVDARYSTDWSAIKGAVKGDLSNYLYKKTKRSPMILPVIMEV"
     gene            complement(2666..>3740)
                     /locus_tag="ACZPAQ_04345"
     CDS             complement(2666..>3740)
                     /locus_tag="ACZPAQ_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR0903247.1"
                     /translation="IAAAGGTPLAHDLECAAQAAWLAEHHQIPVSLFLEQEGDRIYLH
                     LFGRSGLPLGRARERKLEHAVAQGDAPRVPAGQIGEVVRVHAGCDDYAADAARRSRLH
                     RFPMRSITVAVPGSAPADRAIRQALSSLGCTVLDRWRKGVPAFSGLHGGLYLSAQDES
                     GTLLDPGQLLTLVCLIEMEDGGGRVAVPDGASAAVDLVAAGFHGTALRLGRDGEQALS
                     LYAALPWLRDAAFAAARICSRMGSSGEKLEALMSKTPRFSSWKREVPLHGNRGLLMQT
                     LAEGKAAAGGEGVRIHTGNGWVYLVPLSRRPALRILAESPDLEVAAELCDFYAGRAAQ
                     LDRELSRQLPPPEQRESPAGERL"
BASE COUNT          656 a         1193 c         1226 g          665 t
ORIGIN      
        1 aggatcgccc tgcccctgga ggtgctggca gaggccgccg gtctccagct gacctgggac
       61 gaggtggagg gagccgcctg gctgtccact gaccaggccc agcccgcctc tgccagctac
      121 ccggcggagg acctgtactg gctgtccagg attatctcgg cagagagccg gggggaaccc
      181 ctgctgggcc agatcgcggt gggcaacgtg atcctaaacc gggtggagag cagccagtac
      241 cccgacactg tggagggggt ggtctttgac acaaagtacg gcgtccagtt ccagccggtg
      301 tccaacggga ccatctatga cgccccggcc tcctccagcc tggtggcggc gaagctctgc
      361 ctggagggga ccgacgtggt gggcgagagc ttgtattttt tctcccctgc cctgtcggca
      421 ggcagatgga tcgtgagcaa cgccacctat tataccacca tcggtggaca ccagttttat
      481 gtctgaaaaa ctgaggcgcg gagcagatgc tccgcgcctc agtttactct tcttccagat
      541 atccccttcg gcctccggcg gggacacaca ggggaaaaca ggacggaaag ggcccaaccg
      601 ccgggttgac cggctcagac ctccataata acaggcagga tcatggggga gcgcttggtc
      661 ttcttataga ggtagttgga caggtccccc ttgaccgcac ccttgatggc ggaccagtcg
      721 gtggaatagc gggcgtccac gctgttgatg gcctccaggg ccacctggcg cagctcctcc
      781 atcagcccct cggactcctt cacatagacg aagcctcggg tgatcaggtc ggggccggag
      841 accaggctgc cgtcctcccc agacatggac atgaccacca cgatcatgcc gtcctgggcc
      901 agatggcggc ggtcccgcag cactacgctg cccacgtcgc ccactcccaa gccgtccaca
      961 aagacctggc ccgcagtgac agtgcccgcc agacgggcgc tgttctgggt cagttccatg
     1021 acccggccga tgtcgctgat gaggatatga ttggagtcca tccccatctc ccgggccagc
     1081 tttgcgtggg tctggagcat gcgctgctct ccgtggaccg ggatgaagaa cttgggcttg
     1141 accagggcgt ggatgatctt caactcctcc tggcaggcgt ggcccgacac gtggagggcc
     1201 agctcccgct cgttgagcac ctccgccccc ttgcggtaga gctcgttgat gacgttgcca
     1261 atagagtgct cgttgcccgg gatggcggag gcagagatga tgatccgatc cccggggagg
     1321 atatccacct gccggtgggt ggagaaggcc atgcgggaca gggcggacat ggtctcccct
     1381 tgactgccgg tggtgatgat gcacaccctg ttcttgggca gggacttaat ctggttcagg
     1441 tccatcagca ctccatctgg gatgttcata tagcccagct cggtggacac cttcatggcg
     1501 ttctccatgc tgcggccagt gaccgctacc cggcggccat agcgggcggc cacatcgatg
     1561 atctgctgga gccggtccac attgcttgca aaggtagtga cgatgatgcg ctcctcacag
     1621 cccttgaaaa gtgcgtcaaa ggaattgccc acgctgcgct cactgcgggt atagccggga
     1681 cgctccacgt tggtggagtc ggccagcagg gccagcaccc cctcgttgcc cagctggccc
     1741 agccgggcca ggtcgatcat gccgccctgg atgggggtgg cgtcaatctt gaagtcaccg
     1801 gtgtggacgc aggttcccac cgggcacttg atggcaaagg ccaccgcgtc ggcaatggag
     1861 tggttcacat ggatgaattc cacggtgaat ttgccggcct tgatcacgtc gcccgcctcg
     1921 caggtgatga tcttggtctt gtccagcagc cggtgctcct ccagcttcag gcggatcagg
     1981 ccggcgctca tccgggtggc atagatgggg gcgttcaggg agcggaggac atagggcagc
     2041 gcgccgatat ggtcctcgtg tccgtgggtg atgaaaatcc cccggacctt gttctggttt
     2101 ttgatcaggt agctgacgtc ggggatgacc acgtcgatgc cgtacatgtc gtcatcgggg
     2161 aaacccatgc cgcagtctac caccacgatg tcaccgccgt attcatagac ggtgaggttt
     2221 ttgccgatct cgttgagacc gccgagagaa atgatcttca gtttttcagc catacgatac
     2281 tccttgtttt tgatttgtca agttctggtc tatgtacggc tgggcgcaaa acaaccgggc
     2341 gaaggacgcc cctgctttcc ggccctgcct cgccgggaaa cggggtgtct cttcgcctga
     2401 tggagcgggg gccaggggcc gcgccgctcc cgggaggccc gccggggcat ccggcggctg
     2461 cgctctcgtc cgtactgtgt gtccccatgg acgggtgacc gcctgcgtgc gggccctgca
     2521 tgggttatat ttaggcgcgg ggagggggcg cctgccaccc tgacgcgcat aaatccaaaa
     2581 ataagcaaaa tacagtataa cacatggggc tggaaaagta cagcctaaat tttccgccct
     2641 ttttcctgac aggcaggtcc tcggcttaca gccgctcccc ggccgggctc tccctctgct
     2701 ccggcggagg gagctgccgg gacagctccc ggtccagctg ggcggcccgg cccgcgtaga
     2761 agtcgcacag ctcagcggct acctccaggt cgggactctc cgccaggata cgcagggcgg
     2821 gccggcggga cagggggacc agatagaccc acccgttccc cgtgtggatg cggaccccct
     2881 cgccgcctgc agcggccttc ccctccgcca gggtctgcat cagcaggccc cggttcccgt
     2941 ggaggggaac ctcccgcttc caggaggaga agcggggtgt cttgctcatc aaggcctcca
     3001 gcttctcacc ggaggagccc atcctggaac agatgcgggc agcagcaaag gcggcgtccc
     3061 gcagccaggg cagggcggca tacagggaca aggcctgctc accgtcccgg cccagccgca
     3121 gagcggtgcc gtggaatcct gcggccacca ggtccaccgc ggcgctggcc ccatcgggca
     3181 ccgctacccg ccccccgccg tcctccatct cgatcaggca gaccagggtg agcagctgcc
     3241 ctgggtccag aagagtgcca gactcgtcct gagcggagag gtagagcccg ccgtggagcc
     3301 cagagaaggc gggcacccct ttccgccagc ggtccaatac cgtgcagccc aggctggaaa
     3361 gggcctgccg gatggcccgg tcagcgggag cggatccggg gacggccacg gtgatggagc
     3421 gcatggggaa acggtggaga cggctgcgcc tggccgcgtc ggcggcgtag tcatcacagc
     3481 ccgcatggac ccggaccacc tcgccgatct gaccggcggg gacccggggg gcgtccccct
     3541 gggctacggc gtgttccagc ttccgctccc tggcccggcc cagaggcagg ccgctgcggc
     3601 cgaagagatg gagatagatg cggtccccct cctgctccaa aaagagagag acagggatct
     3661 gatggtgctc cgccagccag gcggcctggg cggcgcactc caggtcatgg gccagagggg
     3721 tcccgccggc cgcggcgatg
//
LOCUS       DBMDSJ010000134         3732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6320_length_3732_cov_1.635362, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000134 DBMDSJ010000000
VERSION     DBMDSJ010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6320_length_3732_cov_1.635362"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="ACZPAQ_04350"
     CDS             <1..778
                     /locus_tag="ACZPAQ_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR0903248.1"
                     /translation="EYIARSVRQGMEEADLLPQLTAAVTRFDGMDYRSITSEAGGEEK
                     ELRRVDEGTEIPSTTVKVQENLVKLNKRGRMLVASYEAVRCQKLDLFSVTLRQIGAHI
                     SRMHLEDAIQVLMEGDGNGNAAPVDSTATEDTVTYEDLVDFWAKFDPYEMNTLLVGND
                     VMVKMLKLEEFQNPLTGLNFQGTGRLSTPLGASLLRTSAMPAGTIIGLDRRFALEMVQ
                     GSDVLVEYDKLIDRQLERAAITTISGFAKLFQGAARVLEL"
     gene            775..1113
                     /locus_tag="ACZPAQ_04355"
     CDS             775..1113
                     /locus_tag="ACZPAQ_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903249.1"
                     /translation="MMEEQILAFVELMGGHGNEELLRPLCQAAEREMAGRLREGLSPE
                     ACGPAFPVAAAWLVLAGLEAGTDGVTSFSAGDLTVHTGGDGAGAMRRQAERLMAPYLA
                     DGFAFRGVLG"
     gene            1110..1442
                     /locus_tag="ACZPAQ_04360"
     CDS             1110..1442
                     /locus_tag="ACZPAQ_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903250.1"
                     /translation="MRVETISALLDRYGQCVTLYRGGVEAGREVRAFLQPLREAGREQ
                     ARPSPLGLRREDRYLMLGEPDVPAAPGDRVVWRESGYEVRTAHPIYLGERLSHWWAVL
                     APWGKEET"
     gene            1439..1942
                     /locus_tag="ACZPAQ_04365"
     CDS             1439..1942
                     /locus_tag="ACZPAQ_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903251.1"
                     /translation="MSGGLEQIREGLTAHLNGKGIRAVPAWSGTARTFPCGPVAAVSL
                     RGCRWGPAGFQDYLGERYDPEAGRWEELYGRRVKLTFGLDLYGERAQGDQALQAAFDA
                     LAGALHRKGPPGMRLLELSCGETEYDQGGQLLRRPVEAVYEACLYAAAEPGGLFQDFE
                     IRGGLEP"
     gene            1939..2997
                     /locus_tag="ACZPAQ_04370"
     CDS             1939..2997
                     /locus_tag="ACZPAQ_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HIR0903252.1"
                     /translation="MSVTTHERPGVYSEYDASSVVQGRQGRKMVGMAAIHATAPAGVP
                     QTVTSYEGALIAFGSTGGQDMTELIRLALKNGAAGVVAVPVADEEGYEAAFAALAAMD
                     DIGVVICDSVDREVQQSLRDSAAAASAARRERIAVAAGGAGETVTELLERAKALNSER
                     VVLVAPGGTDEEGKAVSGLSLAAAVAGAIAGTTDPALPLGGAVLSGLYGLETTYGDND
                     LDLLIRGGVTPAERTGGVTSVVRGVTTRTTTDGTADTTWRELSTILVVDDVIPGIRSA
                     LRSKFHRAKNTEQSRGAIRSQVVLELENRKEREIISGYDNVAVSADPDNPTVCQVEFS
                     FSAAQGLNQIWLTAHITV"
     gene            3038..3427
                     /locus_tag="ACZPAQ_04375"
     CDS             3038..3427
                     /locus_tag="ACZPAQ_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903253.1"
                     /translation="MSIAGFPTSSDIYLEVDGVKVAVVQSYSAKASKTSSAVEAFGES
                     EPVATVPGQTSHVLELTRLYATDEAIRDGIDFYGLSGFSLVICKPDRKIIYSDCQWSS
                     ISEAGTLGAMVVEKVTIVASKRIETGV"
     gene            3463..>3732
                     /locus_tag="ACZPAQ_04380"
     CDS             3463..>3732
                     /locus_tag="ACZPAQ_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903254.1"
                     /translation="MELENGMELRLLSALEVLEARREAAQLAESQGERALCSNACLLA
                     RALESGEKPVFDSGRAVLAGLTVSEIAALAGRWREFDRKENPSAAG"
BASE COUNT          643 a         1066 c         1466 g          557 t
ORIGIN      
        1 ggagtatatc gcccgctcgg tgcgccaggg tatggaggag gcggacctgc tgccccagct
       61 caccgccgcg gtgacacggt tcgacggcat ggactaccgc tccatcacct ccgaggcggg
      121 aggcgaggag aaagagctgc gccgagtaga cgaggggaca gagatcccca gcaccacggt
      181 caaggtgcag gagaatctgg tgaagctgaa caagcggggc cggatgctgg tggccagcta
      241 tgaggcggtg cgctgccaga agctggacct gttttccgtc acgctgcggc agatcggcgc
      301 ccacatcagc cggatgcatc tggaggacgc catccaggtg ctgatggagg gggatgggaa
      361 cggcaacgcc gccccggtgg acagcacggc aacggaggac acggtgacct atgaggacct
      421 ggtggacttc tgggccaagt tcgaccccta tgagatgaac acccttctgg tgggcaacga
      481 cgtgatggtg aagatgctca agctggagga gttccagaac ccgctcaccg ggctgaactt
      541 ccagggcacc ggcaggctgt ccacccccct gggggccagc ctgctgcgca cctccgccat
      601 gcctgccggg accatcatcg gcctggaccg gcggttcgcc ctggagatgg tgcagggcag
      661 cgatgtgctg gtggagtacg acaagctcat cgaccggcag ctggagcggg cggccatcac
      721 caccatcagc ggctttgcca agctgttcca gggcgcggcc cgggtgctgg agctgtgatg
      781 gaggaacaga tcctggcctt tgtggaactg atgggggggc acgggaatga ggagctgctc
      841 cgccccctgt gtcaggcagc ggagcgggag atggcgggcc ggctgaggga ggggctgtcc
      901 cccgaggcat gcgggcccgc cttcccggtg gccgcagcct ggctggtgct ggccggactg
      961 gaggcaggga cggacggcgt gacctccttt tccgctggcg acctgacggt acataccgga
     1021 ggagacgggg cgggagccat gcgacgccag gcggagcggc tgatggctcc ctatttggcg
     1081 gacggatttg cctttcgggg ggtgctgggg tgagagtgga aaccatttcc gccctgctgg
     1141 accggtatgg gcagtgtgtg acgctttacc gcggcggcgt ggaggcggga agagaggtgc
     1201 gggccttcct ccagcccctg cgggaggcgg ggagggaaca ggcccgcccc tcccccctgg
     1261 ggctgcggcg ggaggaccgg tatctgatgc tgggggagcc ggacgtcccg gcggcccccg
     1321 gggaccgggt ggtgtggcgg gagagcggat atgaggtccg caccgcccac cccatctatc
     1381 tgggggagcg gctgtcccac tggtgggcag tgctggcccc ctggggcaag gaggagacat
     1441 gagcggcgga ctggaacaga tccgggaggg gctgaccgcc cacctcaatg ggaagggtat
     1501 ccgggcagta cctgcctgga gcgggacggc gcggacgttc ccctgcgggc cggtggccgc
     1561 agtatccctg cgggggtgcc gctggggccc ggccggcttt caagactatc tgggggagcg
     1621 atatgacccg gaggcggggc gctgggagga gctgtatggg cgcagggtga agctcacctt
     1681 cggtctggac ctgtacgggg agcgggcgca gggggaccag gcactccagg cggccttcga
     1741 tgccctggcg ggggcgctcc atcggaaagg tcccccgggg atgcggctgc tggagctctc
     1801 ctgcggggag acggagtatg accagggagg gcagctgctg aggcggccgg tggaggcggt
     1861 atatgaggcg tgcctctatg ccgcggcgga gccgggcggc cttttccagg actttgagat
     1921 cagaggagga ttggagccat gagcgtgacc acacatgagc ggccgggggt gtattcggag
     1981 tatgatgcct cctcggtggt gcagggcaga caggggcgaa agatggtggg gatggctgcc
     2041 atccacgcca cggccccggc aggggtgccc cagacggtga ccagctacga gggcgcgctg
     2101 atcgccttcg gcagcaccgg ggggcaggat atgacagaac tgatccggct ggcactgaag
     2161 aacggggcgg ccggcgtggt ggcggtgccg gtggcggacg aggagggtta tgaggcggca
     2221 tttgccgccc tggcggcgat ggacgatatc ggagtggtca tctgtgacag cgtggaccgg
     2281 gaggtgcagc agtccctgcg ggacagcgcc gccgccgcct ctgccgcccg ccgggagagg
     2341 atcgcggtgg ccgccggcgg ggcgggcgag acggtgacag agctgctgga gcgggccaag
     2401 gcgctgaaca gcgagcgggt ggtgctggtg gcccccgggg gcaccgacga ggaagggaag
     2461 gccgtctccg gcctctccct ggccgcagct gtggccgggg ctattgccgg gacaaccgac
     2521 cccgccctcc ccctgggggg cgcggtgctc agcggactgt atgggctgga gacaacctat
     2581 ggggacaacg acctggacct gctcatccgg ggcggcgtga ctccggcaga gcggacgggt
     2641 ggggtgacct cggtggtgcg gggagtgacc acacgcacca ctaccgacgg gacggcggac
     2701 accacctgga gagagctgtc caccatcctg gtggtggacg acgtgatccc cggcatccgc
     2761 tccgccctgc gcagcaagtt ccaccgggcc aagaacacgg agcagagccg gggggccatc
     2821 cgctcccagg tggtgctgga gctggagaac cgaaaggagc gggagatcat ctccggctat
     2881 gacaatgtgg cggtgtccgc cgacccggac aaccccacgg tgtgccaggt ggagttctcc
     2941 ttctcggcgg cccaggggct gaaccagatc tggctgacgg cccacatcac cgtatgacag
     3001 gccgccggca aaggacagac agtgaggagg gagcagagtg agtatcgcag gatttcccac
     3061 cagcagcgac atctatctgg aggtggatgg agtcaaggtg gcagtggtgc agagctattc
     3121 cgccaaggcc agcaagacca gctcggcggt ggaggccttt ggagagagcg agccggtggc
     3181 cacggtgccg ggccagacca gccatgtgct ggagctgacc cggttgtacg ccaccgacga
     3241 ggccatccgg gacggcatcg acttttacgg gctgagcggt ttctctctgg tgatctgcaa
     3301 gccggaccga aagatcatct attccgactg ccagtggagc agcatcagcg aggcgggcac
     3361 cctgggtgcc atggtggtgg agaaggtgac catcgtggcc tccaagcgca ttgagacggg
     3421 ggtgtgatag tggtgtggtc gattttggcg ggcgcagacc gcatggaact ggagaatggc
     3481 atggagctga ggctgctgtc cgccctggaa gttctggagg cccggcggga ggcggcccag
     3541 ctggcggaga gccaggggga gcgggccctg tgctccaacg catgcctgct ggcccgggcg
     3601 ctggagagcg gagaaaagcc ggtatttgac agtggacggg cggtgctggc agggctgacg
     3661 gtgagtgaga tcgccgctct ggccgggcgg tggagagagt tcgaccggaa ggagaacccc
     3721 tccgccgcag ga
//
LOCUS       DBMDSJ010000135         3726 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6330_length_3726_cov_1.719724, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000135 DBMDSJ010000000
VERSION     DBMDSJ010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_3726_cov_1.719724"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="ACZPAQ_04385"
     CDS             <1..840
                     /locus_tag="ACZPAQ_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903255.1"
                     /translation="KACIGYLPEQPPLYVDMTVREYLEFAAGLKRIPGGERAAQLDRI
                     LEMTRTADVSGRLIRNLSKGYRQRVGLAQALIGFPHILILDEPTVGLDPKQILEIRAL
                     IRELAQEHTVILSSHILSEVQEVCDHIFIIHHGRLVAQGTPAQLERELAGTPAITLLL
                     KAAPEEGRVLLSGVPGVAEVAPLPCGEAGAALLRLTPAEGADPREAVFRACAGADLPI
                     LEMRRESLSLEQIFLELTSDASAAGQAGGRRRPFGRRDSAEPEPAPAPETAITEHGEE
                     AEP"
     gene            837..2873
                     /locus_tag="ACZPAQ_04390"
     CDS             837..2873
                     /locus_tag="ACZPAQ_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7088 domain-containing protein"
                     /protein_id="HIR0903256.1"
                     /translation="MTAVYKRELRSYFTSMVGYVFIAILIFFVGIYFMAYNLFGGYPS
                     FGYVLLSCTIIFMVAVPILTMRSMAEDRRGKTDQLLLTSPVSLTGIVLGKYLAMVTVL
                     LVPILLICFCPLIIAMNGSATLTADYAAILAFFCMGCVYIAVGMFVSALTESQIIAAV
                     GTFAALLVLYLWTDLVSFLPDSLAQLLSSFDFQGVLDNFAYYSVFDLGGLLLYLSMAA
                     VFVFLTVQVLQRRKGITSAATTAVVLAIAVVVNLVVGQLPSDLVERDISDNSLYTVSD
                     TSVDYLSALERDVELVVLASEDTTDQRITKFLHNYAALSGHLSLSFVDPVEHPSALTE
                     YEADQNTVVVRCADTGRQRVVPFSDILVADLMSYYTYGTYTYSEFDAEGQLTSAVDYV
                     TSDNSHILYLAENHGESALGGSVTDAISKANLSTSTVSLLLDNGVPDDCSLLVFNQPQ
                     TDLSAGEAQMVRDYLEGGGQVMILLTRTDLANFNAILADYGLAMAQGYIGDTARYYAQ
                     YGRFYFSATLSASSPITAQFGDDDLTLIYGAHGMTQCDPVRDTITVTPFMTTTESGYS
                     DAGGQTGTYILGAQAEEETDGGTARLTVFTTESVVDQSILTFNPSMVNLDIFLNAATS
                     GMEDISAISIPAKSLEITYNTVLNPGMWSTLYLAVIPLGVLIGGLAYWIKRRKL"
     gene            2873..>3726
                     /locus_tag="ACZPAQ_04395"
     CDS             2873..>3726
                     /locus_tag="ACZPAQ_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025598.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR0903257.1"
                     /translation="MNRQTKQLLALAGVLAVGLGAYAALRVWNAGEEEREAAQADYVL
                     QLSDVTALSFDSGDGPLSFTLEETGWTWDGDSEFPVNGTELDALREDLAALKAERIIP
                     EPDALADYGLEEPAYTLTARTDSGESTTLLLGSLDPGGSHRYAMLDGGTEVYTVDGSI
                     TSSLDSELLDLMELPQLPDLDEKNITSVQLELTDGTVRQLTSAVETRETEVETETGET
                     DENGEPIYETTTETEEITLWSLDGQPLPEGAVGLEDWLYDLSTLYLDSCSAFKADQEE
                     LSACGMDGA"
BASE COUNT          620 a         1330 c         1119 g          657 t
ORIGIN      
        1 aaggcctgca tcggctatct gccggagcag ccgcccctct atgtggacat gacggtgcgg
       61 gagtatctgg agttcgccgc cgggctcaag cggatccccg ggggggagcg ggccgctcag
      121 ctggaccgca tcctggagat gacccggacc gccgacgtgt ccggccggct gatccgcaac
      181 ctgtccaagg gctaccgcca gcgggtgggg ctggcccagg cgctgatcgg cttcccacac
      241 atcctgatcc tggatgagcc cactgtaggc ctggacccca agcagatcct ggagatccgc
      301 gccctgatcc gggagctggc ccaggagcac actgtgatcc tcagctccca catcctctcc
      361 gaggtccagg aggtctgcga ccacatcttt atcatccacc acggccggct ggtggcccaa
      421 ggcactccgg cccagctgga gcgggagctg gcaggcaccc ctgccatcac cctgctgctc
      481 aaggccgccc cggaggaagg ccgggtcctg ctctccggcg tacccggcgt agcggaggtg
      541 gcgccgctgc cctgcgggga ggcgggcgcc gccctgctgc gcctgactcc cgcggagggg
      601 gccgatccca gggaggccgt cttccgcgcc tgtgcggggg cggacctgcc gatcctggag
      661 atgaggcggg agtccctgtc cctggagcag atcttcctgg agctgacctc cgacgcctcc
      721 gccgccggac aggctggcgg ccgcaggcgc cccttcgggc gcagagattc ggcggagccg
      781 gagcccgctc ccgctccgga gacagccatc acggaacacg gagaggaggc ggagccatga
      841 ctgcggtcta caagcgggag ctgcgctcct atttcacctc catggtgggg tatgtgttca
      901 tcgccatcct catctttttt gtaggcatct acttcatggc ctacaacctg ttcggcggct
      961 acccctcctt cggctatgtc ctgctcagct gcaccatcat cttcatggtg gccgttccca
     1021 tcctcaccat gcgctccatg gcggaggacc ggcggggcaa gaccgaccag ctgctgctca
     1081 cctcccccgt ctccctcacc ggcatcgtcc tgggcaaata tctggccatg gtcaccgtgc
     1141 tgctggtccc catcctgctg atctgtttct gcccgctcat catcgccatg aacggctccg
     1201 ccactctgac ggcggactac gccgctatcc tggccttctt ctgcatgggc tgcgtgtaca
     1261 tcgccgtagg catgttcgtc tccgccctca ccgagagcca gatcatcgcc gccgtgggca
     1321 cctttgccgc gctgctggtc ctctacctgt ggaccgatct ggtgtccttt ctccccgact
     1381 ccctggccca gctcctctcc agcttcgact tccagggcgt gctggacaac ttcgcctatt
     1441 acagcgtgtt cgatctgggg ggactgctgc tctatctgtc catggcggcc gtcttcgtct
     1501 tcctcaccgt gcaggtcctc cagcgccgga aggggatcac ctccgccgct accactgcgg
     1561 tggtgctggc catcgccgtg gtggtcaacc tggtggtggg gcagctgccc tctgacctgg
     1621 tggagcggga catctcggac aacagtctct acaccgtcag cgacacttct gtggactacc
     1681 tctccgccct ggagcgggac gtggagctgg tcgtcctggc cagcgaggac accacggacc
     1741 agcgcatcac caaatttctg cacaactatg ccgccctgtc cggccatctc tccctctcct
     1801 tcgtggaccc tgtggagcac ccctccgccc tgacggagta tgaggcagac cagaacactg
     1861 tcgtggtccg ctgtgccgac accggcaggc agcgggtggt ccccttctcc gacattttgg
     1921 tggccgatct catgtcctac tacacctatg gcacctatac ctattccgag ttcgacgccg
     1981 aggggcagct cacttccgcg gtggactacg tcacctcaga caacagccac atcctctatc
     2041 tcgccgaaaa ccacggggag agcgccttgg gcggctcggt caccgacgcc atcagcaagg
     2101 ccaacctgtc tacctccacc gtcagcctcc tgctggacaa cggggtgcct gacgactgct
     2161 ccctgctggt cttcaaccag ccccagaccg acctgtccgc cggcgaggcc cagatggtcc
     2221 gggactacct ggaggggggc ggccaggtga tgatcctgct gacccgcacg gacctggcca
     2281 acttcaatgc catcctcgcc gactacggcc tcgccatggc ccagggctat atcggggaca
     2341 ccgcccggta ctatgcccaa tacggccgct tctacttcag cgccaccctg tccgcctcca
     2401 gccccatcac tgctcagttc ggggacgacg acctgactct gatctacggc gcccacggca
     2461 tgacccagtg cgatccggtc cgtgacacca tcaccgtgac ccccttcatg accaccacgg
     2521 agagcggcta ctccgacgcc ggcgggcaga ccggcaccta tatcctgggc gcccaggcgg
     2581 aggaggagac cgacggcggc accgcccgcc tcaccgtctt caccaccgag agcgtagtgg
     2641 accagagcat cctcaccttc aaccccagca tggtgaacct ggacatcttc ctcaacgcgg
     2701 ccacctccgg catggaggac atctccgcca tctccatccc cgccaagagc ctggaaatca
     2761 cctataacac cgttctcaac ccgggcatgt ggagcaccct ctatctggcc gtcatccccc
     2821 tgggggtcct gatcggcggc ctggcctact ggatcaagcg gaggaaactg tgatgaaccg
     2881 acagacaaaa cagcttttgg ctctcgccgg tgtcctcgcc gtcgggctgg gcgcctacgc
     2941 cgccctgcgg gtctggaacg cgggggagga ggagcgggag gccgcccagg ccgactatgt
     3001 gctccagctc agcgatgtca ccgccctctc ctttgattcc ggggacggcc ccctctcctt
     3061 caccctggag gagacgggct ggacctggga cggggacagc gagttcccgg tgaacggcac
     3121 ggagctggac gccctccgag aggacctggc cgccctgaag gcggagcgga tcattccgga
     3181 acccgacgca ctggctgact acggcctgga ggagcccgcc tacaccctca ccgcccgcac
     3241 tgattccggg gagtccacca ccctgcttct gggcagcctg gaccctgggg gcagccaccg
     3301 gtacgccatg ctcgatggag ggacggaggt ctacacagtg gacggctcca tcacctcctc
     3361 cctggacagc gaactgctgg acctgatgga gctgccccag ctgcctgacc tggacgagaa
     3421 gaacatcacc tccgtccagc tggagctgac cgacggcact gtccgccagc tgacttccgc
     3481 tgtggagacc cgggagacgg aggtggagac cgaaaccggc gagaccgacg agaacgggga
     3541 gcccatctat gagaccacca cggagacaga ggagatcacc ctatggtcgc tggacgggca
     3601 gccgctgccc gagggggccg tcgggctgga ggactggctg tacgacctgt ccaccctata
     3661 cctggacagc tgctccgctt ttaaggcgga ccaggaggag ctgtccgcct gcggcatgga
     3721 cggcgc
//
LOCUS       DBMDSJ010000136         3721 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6345_length_3721_cov_1.388398, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000136 DBMDSJ010000000
VERSION     DBMDSJ010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_3721_cov_1.388398"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            107..1303
                     /locus_tag="ACZPAQ_04400"
     CDS             107..1303
                     /locus_tag="ACZPAQ_04400"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR0903258.1"
                     /translation="MTVMIEPGLLAGAVTPPPSKSQAHRLLIARALAGENLDDPAHRA
                     DSQDIRATRDCLKSLREGEGKLPVLECGESGSTLRFLIPVALALQGGGVFRGRGRLMD
                     RPQEPYFRIFDGMGITHRREGDSLTVRGRLRPGEYPMPGDVSSQFVTGLLFALPLLEG
                     ESIIRLTSPLESRGYVDMTLQVLRDRGIRVEELPEGFRVPGGQQYGRGTLGEEADYSQ
                     AAFWLAAAGLGSPLTVEGLDPDSTQGDRCVVSYFQQLAGPGRVELDIAQCPDLAPALA
                     VRAALRPGQTARLVNAGRLRLKECDRLAAIPEELNKLGGQLTEGADFLEIRGVERLLG
                     GVADSHNDHRIAMMLAVAATRAEGTVTIRGAESVAKSYPDFWEVYESLGGRVLRQREE
                     REACDM"
     gene            1291..2268
                     /locus_tag="ACZPAQ_04405"
     CDS             1291..2268
                     /locus_tag="ACZPAQ_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR0903259.1"
                     /translation="MRYVIFGESHGPAIGVVLEGVPAGLELDLERVSAEMARRAPGGS
                     PLSTARKEADVPEILSGLFEGRTTGTPLCAVIANTDTRSRDYAKLKDLPRPGHGDYAG
                     YVRYQGCNDYRGGGHFSGRLTAPLVFAGAVAKQLLEQKGVQVAARISNVAGIPDPTPE
                     QYEQAVLEARSQLDSVGGCIRCAVTGLPAGLGAPDYGENVEGIFSQYLFAVPAVKAVG
                     FGDGVGFASLRGSQANDSFYMDGDTVKTRTNHSGGVNGGITNGMPVEFEVCIRPTPSI
                     AQPQQTVDLVQRKDAELTITGRHDPCILHRALPVIEAAAALAACQLMDL"
     gene            2297..3091
                     /locus_tag="ACZPAQ_04410"
     CDS             2297..3091
                     /locus_tag="ACZPAQ_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013375.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR0903260.1"
                     /translation="MSDLEQYRQEIDELDEQLVRLFLRRMEVTGKVGEYKLAHGMQVL
                     DRERERRVLAKRASLAVDIRQEEDVKELFQTIVAISRRRQQELIDRSGTGTVRKTNVV
                     LIGMPGSGKSCVGAALAERLGMPLVETDAMVVEKTGRSIPDIFAQDGEAAFRREETAA
                     ARRAAAMEGTVISTGGGIILRPENMEILSATGTVYFLDRDPRDIANTELDGRPLLSGG
                     RDRVFLLYDQRINLYRKYARYTVPSTTVDETVDSVLALVRRGQDVR"
     gene            3167..>3721
                     /locus_tag="ACZPAQ_04415"
     CDS             3167..>3721
                     /locus_tag="ACZPAQ_04415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020090.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR0903261.1"
                     /translation="MMEKTEQGLGRLCVIGDPVEHSKSPLIQNAMIAKLGIPYHYTKR
                     LVHQGDTQEWLDTARREGFAGFNATMPHKVALVPLMDELGEDARLYGAVNTVCIRGKR
                     CIGHNTDGMGFYQALTDLGVEVPGRRITLLGAGGAAKAVALKLVQQGAGPITVCNRTP
                     QKAEALCNLAKGRMGHIGFALEDLR"
BASE COUNT          621 a         1145 c         1397 g          558 t
ORIGIN      
        1 ggccctctcc gtgtgatcag atctggatgc tgatgcgggg gacacatcga tttcatcggt
       61 gtgtcccccg tttttttgca gcacagcgag aaaaggagcg aaacatatga cagtgatgat
      121 cgagcctggg ctgctggccg gggcggtgac gccgcctccc tccaagagcc aggcccaccg
      181 gctgctgatc gcccgggcgc tggcggggga gaacctggac gaccccgccc accgggcgga
      241 ttcccaggat atccgggcca cccgggactg cctgaagagc ctgcgggaag gggaggggaa
      301 gctcccggtg ctggagtgcg gagagtcggg ctccacactg cggttcctga tcccggtggc
      361 gctggccctg cagggcggag gcgtgtttcg gggacggggg aggctgatgg accggcccca
      421 ggagccctac ttccggatct ttgacgggat ggggatcacc caccgccggg agggggactc
      481 cctgacggtc cggggccggc tgcgtcccgg ggagtatccc atgccggggg atgtgtcttc
      541 tcagttcgtc acgggcctgc tctttgccct gccgctgctg gagggggaga gcatcatccg
      601 gctcacctcc ccgctggagt ccagagggta tgtggacatg accctccagg tgctgcggga
      661 ccggggcatc cgggtggagg agctgcccga gggcttccgg gtgcccggag gccagcagta
      721 cggccgcggc actctggggg aggaggcgga ctactcccag gccgccttct ggctggctgc
      781 cgccgggctg gggagcccgc tgacggtgga gggcctggac ccggactcca cccaggggga
      841 ccggtgtgtt gtatcctatt tccagcagct ggccgggccg ggccgggtgg agctggacat
      901 tgcccagtgt ccggacctgg ctccagccct ggcggtgcgg gccgccctcc ggcccgggca
      961 gacggcccgg ctggtcaacg cggggcggct gcggctgaag gagtgtgacc gcctggcggc
     1021 tatcccggag gagctgaaca agctgggcgg ccagctcacc gagggggcgg attttctgga
     1081 gatccgcggc gtggaacggc tgctgggggg cgttgcggac agccacaacg accaccgcat
     1141 cgccatgatg ctggcggtgg ccgccacccg ggcggaaggg acagtgacca tccggggcgc
     1201 ggagagcgtg gccaagtcct atcccgactt ctgggaggtc tatgagagcc tgggcgggcg
     1261 cgtcctgcgg cagagagagg agcgagaagc atgcgatatg tgatctttgg agagtcccac
     1321 ggccccgcca tcggtgtggt gctggagggg gtgcctgcgg ggctggagct ggacctggag
     1381 cgggtgtccg ccgagatggc ccgccgggcc ccggggggaa gtcccctgtc caccgcccgg
     1441 aaggaggcgg atgtgccgga gatcctctcc ggcctgtttg agggcaggac cacgggcacc
     1501 ccgctgtgcg cggtgatcgc aaataccgac acccgttcca gggactacgc gaagctgaag
     1561 gacctgcccc gtccaggaca cggggactac gccggctatg tgcgttatca ggggtgcaac
     1621 gactaccggg gcggaggcca tttctccggc cggctcactg cgccgctggt ctttgcaggg
     1681 gcggtggcca agcagctgct ggagcagaag ggagtccagg tggccgcccg catctccaac
     1741 gtggcgggca tcccggaccc cacgccggag cagtatgagc aggcggtgct ggaggccagg
     1801 agccagctgg actcggtagg cgggtgcatc cgctgtgccg tcaccggtct gcccgccggc
     1861 ctgggggcgc cggactacgg ggagaacgtg gagggcatct tctcccagta cctcttcgcg
     1921 gtgcccgcgg tgaaggcggt gggcttcgga gacggagtgg gctttgcctc cctgcggggg
     1981 agccaggcca acgacagctt ttatatggat ggagacacag tgaagacccg gaccaaccac
     2041 tccggcgggg tgaacggggg catcaccaac gggatgccgg tggagttcga ggtatgcatc
     2101 cggcccaccc cctccatcgc ccagcctcag cagacggtgg atctggtcca gcgaaaagac
     2161 gcggagctga ccatcaccgg ccggcacgac ccctgtatcc tgcatcgggc gctgcccgtg
     2221 atcgaggccg ccgccgccct ggctgcctgc cagctgatgg acttatgacc gatttggaca
     2281 gagagaggga cgcgatatga gcgatttgga acagtacaga caggagatcg acgagctgga
     2341 cgaacagctg gtccggctct tcctccggcg gatggaggtc actggcaagg taggggagta
     2401 caagctggcc cacgggatgc aggtgctgga ccgggagcgg gaacgccggg tgctggccaa
     2461 gcgggccagc ctggcggtgg acatccggca ggaggaggac gtaaaggagc tattccagac
     2521 catcgtggcc atcagccgcc gccggcagca ggagctgatc gaccgcagcg gcactggcac
     2581 ggtccgaaag accaatgtgg tgctcatcgg catgccgggc agcgggaagt cctgcgtggg
     2641 ggcggccctg gcggagcggc tggggatgcc cctggtggag acggacgcca tggtggtgga
     2701 aaagacgggg cgctccatcc cggacatttt tgcccaggac ggggaggccg ccttccgccg
     2761 ggaggagacc gccgccgccc gccgggccgc ggccatggag gggacggtca tctccaccgg
     2821 cggcgggatc atcctccggc cggagaatat ggagatcctc agcgccaccg gcaccgtcta
     2881 tttcctggac cgggacccca gggacatcgc caacacggag ctggacggcc ggcccctgct
     2941 cagcgggggg cgggaccggg tattcctgtt gtatgatcag cgcatcaacc tgtaccgcaa
     3001 atatgcccgg tacaccgtcc ccagcaccac ggtggatgag acggtggact ccgtcctggc
     3061 cctggtgcgg cggggccagg acgtgcgctg accgagaagg agcgccgccg tgtcccgccg
     3121 cccggagagg ggcgggagac cggcgtggga aagtgaggct gacatcatga tggaaaaaac
     3181 ggagcagggc ctgggcaggc tgtgcgtcat cggtgacccg gtggagcact ccaaatcgcc
     3241 cctgatccag aacgcgatga tcgcgaagct gggcatccct tatcactaca ccaagcgcct
     3301 ggtccaccag ggggataccc aggagtggct ggataccgcc cgccgggagg gcttcgcggg
     3361 ctttaacgcc actatgcccc acaaggtggc cctggtccca ctgatggatg agctggggga
     3421 ggatgcccgg ctgtacggcg cggtcaacac cgtgtgcatc cggggaaagc ggtgcatcgg
     3481 ccacaacacc gacgggatgg gtttctacca ggctctcacc gacctggggg tggaggtgcc
     3541 gggccgccgc atcaccctgc tgggcgcggg gggcgcggcc aaggctgtgg ccctgaagct
     3601 ggtgcagcag ggggcgggcc ccatcaccgt gtgcaaccgg accccccaga aggcggaggc
     3661 cctgtgcaat ctggcaaagg gccggatggg gcacatcggt ttcgcccttg aggacctgcg
     3721 c
//
LOCUS       DBMDSJ010000137         3715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6354_length_3715_cov_1.609297, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000137 DBMDSJ010000000
VERSION     DBMDSJ010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6354_length_3715_cov_1.609297"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(423..1043)
                     /gene="spoIIR"
                     /locus_tag="ACZPAQ_04420"
     CDS             complement(423..1043)
                     /gene="spoIIR"
                     /locus_tag="ACZPAQ_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HIR0903262.1"
                     /translation="MDRKMKRWELALMCGVLCALALGGWLDREQERLADQVIRLHVIA
                     NSDSPEDQALKLEVRDQVLAHAEELYPAGADRTAAAAAIQEHLPELAAAGQRVLEERG
                     CDYAVRAELTETWFPTKEYEDFALPAGEYTALRVVIGEGAGQNWWCVAFPPLCLGAAS
                     ETVEEAAQAGRFTDDQAALMTGETQGYVLKFKAIELWEGLKRLTGG"
     gene            complement(1117..1965)
                     /gene="ispE"
                     /locus_tag="ACZPAQ_04425"
     CDS             complement(1117..1965)
                     /gene="ispE"
                     /locus_tag="ACZPAQ_04425"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR0903263.1"
                     /translation="MERFAPAKLNLTLDVLGKRPDGYHDLKMVMQSITLGDTLTLEPG
                     GEGVRVQTDLHFLPTGPKNLAAAAALRFYETLGRPVPGLEISIRKRIPVCAGMAGGST
                     DAAAVLRALNEKEGNPFSMEELARVGEGVGSDVPYCVLGGTALAEGRGEMLTPLPPLP
                     RCWVAVCKPDFPVSTPELFARIDGMRVRCRPDTAGLLAALAAGDLPGVARRMYNVFEK
                     VLPERQEVRVAEIKHTLIQCGALGATMSGTGPTAFGLFQRKELAEEACALLSEQNRDT
                     FLAETV"
     gene            complement(1985..3271)
                     /locus_tag="ACZPAQ_04430"
     CDS             complement(1985..3271)
                     /locus_tag="ACZPAQ_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR0903264.1"
                     /translation="MNDLFETLPFRPADILLPQNCDLGLWSVVACDQYTSQPEYWQRV
                     EERVGRAPSSLRLILPESCLDGPSVETDIMEINNTMSRYLREERFALLPQTMIYVERT
                     LHNGKLRRGLVGMVDLEQYDYEPGSPAQVRATEGTVLSRIPPRVAVRKNAPIELPHVM
                     LLTDDPERTVIEPLAREKAGMEQVYDFDLMERGGHLAGWKLGREQLSAVAGALQALAD
                     PAAFNARYGTQDQPVLLFAVGDGNHSLATAKECYERQKRITPQDQWETLPSRYALAEL
                     VNLHDESLEFEPIHRVVFGAEPAQVLSALRMAYPDAREGEGDGHVIRYLYGNARGALT
                     VPDPAAQLAVGTLQSFLDRWMEGRSGVSLDYIHGAEVVEELTRRPKAIGFLLPPMKKE
                     ELFPTVIHDGVLPRKTFSMGEAHDKRFYLEARKIRL"
BASE COUNT          615 a         1261 c         1088 g          751 t
ORIGIN      
        1 ccgccatcag gccgtagtag ttggcgtcca tcacatcctc gtagtaggat gcgctccggc
       61 tcacgtcctg gaacttctgc tccggctgat acgccgcgct tccaggcacc gccatggcca
      121 gagacagcgc cgccgcagtg gacacagtca caaatctctg catctttctt ttcatcttgt
      181 ttgcttcctc cctaaatttg ttcacgctct gcaaaataga tgtcttgttc ctccgcttta
      241 ccgcctccca tttttcgttg ttttccataa tttttgctgc ttggactgat gtctttcatt
      301 atatcctgtt caatacattt tttctagtgt taaattatag atagtaccat gcaaaagatt
      361 cattgaatct tttgcaaatg acaggcgggg gaggccctcc ggcctccccc gcccgttccc
      421 tttcaacccc cggtcagtct tttcagcccc tcccagagct cgatggcctt gaacttcagc
      481 acatatccct gggtctcgcc agtcatcagg gcggcctggt catcggtgaa gcgtcccgcc
      541 tgggccgcct cctccaccgt ctcgctggcc gcacccaagc acaggggcgg gaaggccacg
      601 caccaccagt tctgccccgc tccctcgccg atgaccaccc gcagggccgt gtattctcct
      661 gccggaaggg caaagtcctc atactccttg gtggggaacc aggtctctgt cagctccgcc
      721 cggaccgcgt agtcgcagcc ccgctcctcc agcacccgct gtcctgccgc tgccagctca
      781 ggcagatgct cctggatggc cgccgccgcc gccgtgcggt ctgcccccgc cgggtacagc
      841 tcctccgcgt gggccaggac ctggtcccga acctccagtt tcaatgcctg gtcctccggg
      901 ctgtctgaat tggcgatgac atggaggcgg atcacctggt ccgccagccg ctcctgctcc
      961 cggtccagcc atccccccag tgccagtgcg cacagcacgc cgcacatcag cgccaactcc
     1021 cagcgcttca tttttcggtc catatacaac accatccata cctttcgtat tttccgagtg
     1081 gaattctacg aagagtatgg acagtgtctt tccttcttaa accgtttccg ccaaaaatgt
     1141 atcccggttc tgctctgaca gaagggcaca ggcctcctct gccagctcct tccgttggaa
     1201 caggccaaag gcggtggggc ccgtcccgct catggtggcg cccagggctc cgcactggat
     1261 cagtgtgtgc ttgatctctg ccacccttac ctcctgccgc tccggcagca ctttctcaaa
     1321 cacgttgtac atgcgccggg ccacaccagg caggtccccc gccgccagcg ctgccagcag
     1381 tccggcggtg tcgggccggc agcggacccg catcccgtcg atgcgtgcaa acagctctgg
     1441 cgtggagacg gggaaatccg gcttgcacac agccacccag caccggggca gcgggggcag
     1501 tggggtcagc atctcccccc gcccctcggc caaggcggtg ccgcccagta cgcagtaagg
     1561 gacgtcggag cccacccctt cccccacccg tgccagctcc tccatggaga agggattccc
     1621 ctccttctcg ttcagggccc gcagcacagc ggcggcatcg gtgctgccgc cggccatccc
     1681 ggcgcacacc gggatgcgct tgcggatgga gatctccagt cccggcaccg gtcgtcccag
     1741 cgtctcatag aagcggagcg cggcggcggc ggccaggttc ttgggccccg tgggcaggaa
     1801 atgcaggtcg gtctgcaccc gcacgccctc cccgcccggc tccagggtga gggtatcccc
     1861 cagggtgata gactgcatca ccatcttcag gtcgtggtag ccgtcaggcc gctttcccag
     1921 cacatccagc gtcaggttca gcttggcggg ggcaaatcgt tccatcgtcc gcacctccac
     1981 cggttcacag ccggatcttc cgggcctcta agtaaaagcg cttgtcgtgg gcctccccca
     2041 tggagaaggt ctttcggggc agcactccgt cgtggatcac ggtggggaac agctcctcct
     2101 ttttcatggg cggcaggagg aagccgatgg ccttgggccg ccgggtcagc tcttccacta
     2161 cctcggcccc gtggatatag tccaggctga cgccggaccg cccctccatc caccggtcca
     2221 ggaaggactg aagggttccc accgccagct gggcggcggg gtcagggacg gtgagagccc
     2281 cccgggcgtt cccgtagagg tagcggatca catgtccgtc cccctccccc tcccgggcat
     2341 cggggtaagc catgcgcagg gcggagagca cctgggccgg ctccgcgccg aagaccaccc
     2401 ggtggatggg ctcgaactcc aaggactcgt cgtggaggtt gaccagctcc gccagggcgt
     2461 accgggaggg cagggtctcc cactgatcct ggggcgtgat gcgcttctgc cgctcatagc
     2521 actccttggc ggtggccagg gagtggttcc cgtctcccac agcgaacagg aggacaggct
     2581 ggtcctgggt cccgtagcgg gcgttgaagg cggcgggatc agccagagcc tggagggcgc
     2641 cggccacggc ggagagctgc tccctcccca gcttccaacc ggccaggtgg ccgccccgct
     2701 ccatcaaatc gaagtcatag acctgctcca tgcccgcctt ctcccgggcc aggggctcga
     2761 tgacggtgcg ctccggatcg tcagtgagga gcatgacgtg aggcagctca atgggggcgt
     2821 tcttccgcac cgccactcgg ggcgggatac gggagaggac ggtgccctcg gtggcccgca
     2881 cctgggccgg ggagcccggc tcatagtcgt actgctccag gtccaccatg cccaccagtc
     2941 ctctgcggag ctttccgttg tgcagggtgc gctctacata gatcatggtc tggggcagaa
     3001 gggcaaacct ctcctcccgc agatagcggg acatggtgtt gttgatctcc atgatgtcgg
     3061 tctccacgct gggaccgtcc agacagctct ccggcaggat gagccgcagg gatgagggag
     3121 cccgccccac cctctcttct acccgctgcc agtattccgg ctgtgaggtg tactggtcac
     3181 aggccaccac gctccacagg cccaagtcgc agttttgggg gagcaggata tccgccggac
     3241 gaaaggggag ggtttcaaat aaatcattca tcaatggtca ctcactttaa tcgttctctt
     3301 gatgcagggg gatttttccg gccttggccg ttttggttct ttccgcaggg cgcgatgacg
     3361 tggcgcgccg tcttgggtag ttttcgcagg tgggtcgccg cctgcgggcg gcaagtgact
     3421 ttcccagcgc cgggaaagtc accaaaggat cgccgggggg cagctcagga tgatcgcttc
     3481 acgctcatat ccgccttacc ccgggtcctt cattacgggg acacgctcct gtttggtcat
     3541 acgttatttc cggctcgcaa aatctgagtg gctggtctaa attcccaccg ggccactggg
     3601 ccctggtctg cagaaattca gggcgcatgc aattccacaa ctgcgcctgg ctttgctaag
     3661 ccgacactcc cggtgcgaat cctggcatgc cggtctggga ccggcctcta cggta
//
LOCUS       DBMDSJ010000138         3707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6369_length_3707_cov_1.684138, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000138 DBMDSJ010000000
VERSION     DBMDSJ010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6369_length_3707_cov_1.684138"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="ACZPAQ_04435"
     CDS             complement(<1..1077)
                     /locus_tag="ACZPAQ_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016800.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR0903265.1"
                     /translation="MNRAIEIWNSSILGRVLTAICLWFSGQWQRSRVVRAFVAPMAGR
                     QAAESSVFFRLWDLIRGGLCALYRRLGLERLFGGSIFLCLTLWCSAAVVLAPLLPTMA
                     VLALAAVAFASLALNLVRDRARPLAYSPVNRYILLYAAVYLAATATSVTPRQSLFPGL
                     LMVFFMAFALVVENAVDSWERAEQLVFLMVLAGGVVALLGVGQYVLGATGDAAWVDSD
                     MFSSVTIRVYSTLQNPNVLAEYLILMISLGGAALLTAKGTGKRLVLLACCGVMCLCMV
                     LTMSRGGWLGLLIAGMIFLVLLNPRVLVLAPVVLPVALVALYFVLPDTVTSRFTSIGN
                     LEDTSTSYRVSIWMGTIAMLKDYWL"
     gene            complement(1082..2641)
                     /gene="murJ"
                     /locus_tag="ACZPAQ_04440"
     CDS             complement(1082..2641)
                     /gene="murJ"
                     /locus_tag="ACZPAQ_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR0903266.1"
                     /translation="MEERQDHATRTISIVMIIALVGKVLGLYRDHLLAVYYGTGMAAN
                     AFNTASRIPRVFFDAVFASAISACFIPVFSEYLTRKGREPAFRFAGCFITVMGLLSAV
                     LSVLGILFAGPLVSLFADGYDAQTAELAVSLTRVMFPTVLFTGVAFSFVGVLQSLGEF
                     NVPALISVVSNLVIILYYLTLDQQFGIYGLAVAFLVGWFLQAAVQVPSLRRLGFRYRP
                     GLELRSEGMRKVFALMLPVMVSTWVQPINLTINSKFGSRLYEGAGVSAIEFSTNLYLV
                     IAGVFVLSVTNVIFPKLSRLTGREQEGEFRETLRTTLHGCLFFVLPMSAGLMVLSRPL
                     ISFIYGGGAFDDFSVSITSQGLFWVSLGMTGYAVQNILSRAYFARQKGKVPLAAGAAS
                     ILVNVLLCMALAQRMGVAGLALSSAVSSTVYALLLDIPLELRGEGVLSRSFLLDLGKM
                     ALATALMAVGAWAVAGLTEGLLPGKAGELLTLGASAAAGVAVYFPAAVLLRLDEGQLA
                     LKMVKRVWKRG"
     gene            2855..>3707
                     /locus_tag="ACZPAQ_04445"
     CDS             2855..>3707
                     /locus_tag="ACZPAQ_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903267.1"
                     /translation="MKHRHFTQAARRLTALTLALLLALPSAYAAAGSPQLRTTRELAD
                     GLTYSNTITSHSAAGRVESFSLELSPDSREVEPIFLQASGTAYGAGSINLAVSYAQSL
                     GYHVLGAINTDFFAPSTGVPLGISIEDGIYKSSPEAEAAVVITDGEVELVEQSQVTLT
                     LTNESTGGQTVLTHLNKYRADSGGLYLFNYDFSTVSTHTSTPGWMVRMELTDPDDTLS
                     VSGRLELEVTELLRTDTSMVIGENEYILTAADAGGLESVFQSFQVGDRVTLHTDCTSS
                     TLEDAQWA"
BASE COUNT          785 a         1296 c         1064 g          562 t
ORIGIN      
        1 cagccagtag tccttcagca tggctatggt gcccatccag atggacaccc ggtaggacgt
       61 ggaggtgtcc tccaggtttc cgatgctggt gaagcggctg gtgacggtat ccggcagcac
      121 aaagtagagg gccaccagcg ccactgggag caccaccggc gccagcacca gcacccgggg
      181 gttgaggagc accaaaaaga tcatccccgc gatcagcagg cccagccagc cgccgcggga
      241 catggtgagc accatgcaca ggcacatcac cccgcagcag gccagcagca ccagccgttt
      301 cccggtcccc ttggcggtga gcagggccgc cccgcccagg gagatcatca aaatcagata
      361 ctcggccagc acattggggt tctggagggt ggagtagacc cggatggtga ccgaggagaa
      421 catgtcgctg tccacccagg cggcatcccc cgtggcgccc agcacatact ggcccacgcc
      481 cagcagagcc accacgcctc ccgccagcac catgagaaac accagctgtt ccgcccgctc
      541 ccagctgtcc acggcgttct ccaccaccaa ggcgaaggcc atgaaaaaga ccatcagtag
      601 gccggggaac agactctgcc ggggcgtgac ggaggtggcc gtggccgcca gatacaccgc
      661 cgcgtacagc aggatatacc ggttgaccgg ggagtaggcc aggggccgtg cccggtcccg
      721 gaccagattc agtgccaggg aggcgaaagc caccgccgcc agagccagta ccgccatagt
      781 gggcagcagg ggggccagca ccaccgccgc agaacaccag agggtcaggc acaaaaagat
      841 gctccccccg aacaggcgct ccagtcccag cctgcggtac agggcgcaca gcccgccccg
      901 gatcaggtcc cacagtcgga aaaagacact ggattccgcc gcctgtcggc ccgccattgg
      961 ggccacgaag gcccggacca cccgacttct ctgccactgt ccgctgaacc acagacagat
     1021 ggcggtgagc acccgcccca gtatgctgct gttccagatc tctatcgctc gattcattgc
     1081 atcagcctcg tttccacact cgtttcacca tcttcagggc cagctgcccc tcgtccaatc
     1141 tcagcagcac cgccgccggg aagtagacgg ccacgccagc cgccgccgaa gcccccaggg
     1201 tgagcagctc ccccgccttg ccgggcagca ggccctctgt caggccggcc accgcccagg
     1261 cgcccaccgc catcagcgcg gtggccagcg ccatcttccc caggtccagc aggaaactcc
     1321 ggctgaggac cccctccccc cgcagctcca gggggatgtc cagcaacagg gcgtagacgg
     1381 tggaggatac agcggaggag agggccagac cagccacccc catcctctgg gccagcgcca
     1441 tgcacagcag gacattgacc aggatggagg ccgcaccggc cgccaggggc accttcccct
     1501 tctgccgggc gaagtaggcc cggctgagga tattctggac cgcgtatccc gtcatcccca
     1561 gcgagaccca gaacagtccc tgggaggtga tggagacgga aaaatcatca aaagcgcctc
     1621 ctccataaat aaaggagatc agcgggcggg agagcaccat caaccccgcg gacatgggca
     1681 ggacgaagaa cagacagccg tgcagggtgg tccgcagcgt ctcccggaac tccccctcct
     1741 gctcccggcc ggtgaggcgg gacagcttgg gaaatatcac attggtgacg gacaggacga
     1801 acaccccggc gatcaccaga tacaagttgg tggaaaactc gatggcggac actccggccc
     1861 cctcgtacag tcgggacccg aacttggagt tgatagtcag gttgatgggc tgcacccagg
     1921 tggagaccat cactggcagc atcagggcaa agaccttccg catcccctcg gagcgcagct
     1981 ccagccccgg ccggtagcgg aagcccagcc ggcgcagtga gggcacctgg accgccgcct
     2041 ggaggaacca gcccaccaga aaggcaacgg ccaggccgta gatgccgaac tgctggtcca
     2101 gcgtcaggta atagagaatg atgaccaggt tggacaccac gctgatcagg gcagggacat
     2161 tgaactcccc cagggactgg agcactccca caaaggagaa ggccaccccg gtgaacagca
     2221 ccgtggggaa catcacccgg gtgagggaga cggccagctc cgccgtctgg gcatcatatc
     2281 cgtcggcaaa caggctgacc aggggccccg cgaacaggat gcccagcacc gacaggaccg
     2341 cagagagcag ccccatcacg gtgatgaagc agcccgcgaa gcggaaggcc ggctcccgcc
     2401 ccttccgggt gagatactcg ctgaacaccg ggataaagca ggccgagata gcggaggcga
     2461 acaccgcatc aaaaaacacc cttgggatcc ggctggctgt attgaaggcg ttagccgcca
     2521 tccccgtccc gtagtaaacc gccagcagat ggtcccggta cagccccagc accttgccca
     2581 ccagggcgat gatcatcacg atgctgatgg tccgtgtggc atggtcctgc cgctcctcca
     2641 tcgcgccacc tcctatggat ccccaataaa atcgttttat tttacaccac ccctccgact
     2701 ttttcaaccg ttggcccgtg tgttcatagg atgttcacat tttcccgaaa tctgtacact
     2761 tttttccatt gaaagtgccc tgctccactg ctataataag gaattagttt tgctgtcccc
     2821 accacctgtc ctaaccatgt gaaaaggagt atccatgaaa catcgtcatt tcacccaagc
     2881 tgcccgccgc ctcaccgcct tgacgctggc gctgctgctg gcactgccct cggcctacgc
     2941 cgccgcaggc tccccccagc tgcgcaccac ccgggagctg gctgacggcc tgacctattc
     3001 caacaccatc accagccact ccgccgcggg ccgggtggag agcttctccc tggaactctc
     3061 tccggatagc cgggaggtgg agcccatctt cctccaggca tccggcaccg cctacggggc
     3121 cggctccatc aatctggcgg tcagctatgc ccagagcctg ggctaccacg tgctgggcgc
     3181 catcaatacc gacttctttg ctccctccac cggcgtgcct ctgggcatct ccattgagga
     3241 cggcatttat aaatccagcc cggaggccga ggccgccgtg gtcatcaccg acggtgaggt
     3301 ggagctggta gagcagtccc aggtcaccct caccctcacc aacgagtcca ccggaggcca
     3361 gaccgttctg acccacctga acaaataccg ggccgactcc ggcgggctct acctgttcaa
     3421 ctacgatttt tccaccgtct ccacccacac cagcaccccc ggctggatgg tgcgcatgga
     3481 gctgaccgat cccgacgaca ccctctccgt ctccggtcgt ctggaattgg aggtcactga
     3541 gctgctccgc accgacacct caatggtcat tggcgagaac gagtacatat tgactgccgc
     3601 cgacgccggc ggcctggagt ccgtattcca gagcttccag gtgggcgacc gggtcaccct
     3661 ccacaccgac tgcacctcct ccaccctcga ggacgcccag tgggcca
//
LOCUS       DBMDSJ010000139         3706 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6373_length_3706_cov_1.589736, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000139 DBMDSJ010000000
VERSION     DBMDSJ010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6373_length_3706_cov_1.589736"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="ACZPAQ_04450"
     CDS             <1..702
                     /locus_tag="ACZPAQ_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0903268.1"
                     /translation="NGLLKPTSGQVLFQGEDIWADAKRTRQTRFQVGLVFQYPEYQLF
                     EETVYKDISFGPKNMGLDEGEVDRRVREAARFVGLRDDQLEKSPFELSGGQKRRVAIA
                     GVIAMEPRVLILDEPTAGLDPVGVESILGNIRDYHQSNNATIILVSHSMEEVARTVDR
                     LVVVNDGRIPFQGPPREVFRHGAELERMGLGVPQMTRVFSRLRAMGVDIDASVYIIPQ
                     AKETILRRLREKGVE"
     gene            703..1512
                     /locus_tag="ACZPAQ_04455"
     CDS             703..1512
                     /locus_tag="ACZPAQ_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR0903269.1"
                     /translation="MALKDITLGQYFPGNSIVHRLDPRTKLIAVVLYIVALFLAKSFV
                     TYGIMFLLLAVSIAVSKVPVKSIFRGMKPVVFIVIFTAILNLFYTPGDTVLVRFWIFT
                     ITLEGVFNAFFMVVRILMLIAGTFLLTYTTSPILLTDGLENLLGPLKKIHVPVHELAM
                     MMSIALRFIPLLIEETDKIMSAQRARGADFESGNLIQRAKALVPLLVPLFISAFRRAD
                     ELAIAMECRCYHGGEGRTRLRQLKYRRNDAAVILLFLVLTAGVGVLGRFGL"
     gene            complement(1517..1744)
                     /locus_tag="ACZPAQ_04460"
     CDS             complement(1517..1744)
                     /locus_tag="ACZPAQ_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903270.1"
                     /translation="MSNEIYKFCNGVPDYVRAEREYLQIARQVADRLGYDLYNEFEEK
                     LNDYMAQQLDACYLFGLGLRRELAAGLMLEE"
     gene            1877..2098
                     /locus_tag="ACZPAQ_04465"
     CDS             1877..2098
                     /locus_tag="ACZPAQ_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903271.1"
                     /translation="MENFSARLRLLRKERGLKQREMAEICGLKLRSYQDYEYQVSYPS
                     ALGLVALADFFGVSLDYLMGRTDKREINR"
     gene            2142..2894
                     /gene="truA"
                     /locus_tag="ACZPAQ_04470"
     CDS             2142..2894
                     /gene="truA"
                     /locus_tag="ACZPAQ_04470"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR0903272.1"
                     /translation="MERNIALKLMYVGTAYHGWQVQKNAVTVAETLERALSTVVCHPV
                     KCTGAGRTDAGVHAQVYVANFRTTSTIPCDRLPLAVNTRLPDDIVVVRAVQVPMEFNA
                     IGSCRKKEYTYRIYNSRLGNAFYVDRAWFYPKHLDESVMQRAASQFVGTHDFRAVRSV
                     GTETRTTVRTVHYFQVERKGELIECRVCADGFLYNMVRAMVGTVVYAAEGKLAPEDIP
                     AILDSGNRTLAGPTVPPGGLYMTRLWYDEDVV"
     gene            2937..>3706
                     /locus_tag="ACZPAQ_04475"
     CDS             2937..>3706
                     /locus_tag="ACZPAQ_04475"
                     /inference="COORDINATES: protein motif:HMM:NF039834.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HIR0903273.1"
                     /translation="MKDQTTKQASASQKPNILVRLLALLVTAALALGALTLVVYRDQF
                     NLDAVKRWLALRSVETDASGAAAPFTHAGGDSISFAYLKGGILQCSAAGAHYYSFSGD
                     QYAEEVTPLSQPVLSASSSAGVVYDAGGESLFLFQDGQEAFSLDVDGTLLSARVNDSG
                     WLAVTAQQSGYKGSVTVYNVNSTSRKEIIQLNFSSVFVADAAVSPDSKTVAVITIGQE
                     NGSFFSQLLLYPVDSSEPSATLSLGNLAVLDLDYEAEQL"
BASE COUNT          651 a         1224 c         1113 g          718 t
ORIGIN      
        1 aacggcctgc tcaagcccac ctccggccag gtgctcttcc agggggagga catctgggcg
       61 gatgccaagc gcactcgtca gacccggttc caggtggggc tggtgttcca atacccggag
      121 taccagctct ttgaggagac cgtctacaag gacatctcct tcggcccgaa aaacatgggg
      181 ctggacgagg gggaggtgga ccgccgggtg cgggaggccg cccgcttcgt gggactgcgg
      241 gacgaccagc tggaaaagtc cccctttgag ctctccggcg gccagaagcg gcgggtggcc
      301 atcgcggggg tcatcgccat ggagcccagg gtgctcattc tggacgagcc caccgccggg
      361 ctggacccgg tgggagtgga gtccatcctg ggcaacatcc gggactatca ccagtccaac
      421 aatgccacca tcatcctggt ctcccactcc atggaggagg tggcccgcac ggtggaccgg
      481 ctggtggtgg tcaacgacgg ccgcatccct ttccagggcc cgccccggga ggtgttccgc
      541 cacggtgcgg aactggagcg catggggctg ggcgtgcccc agatgacccg ggtgttcagc
      601 cgtctgcggg ccatgggcgt ggacatcgac gcctccgtct acatcatccc ccaggccaag
      661 gagaccattc tgcgcaggct tcgggaaaag ggggtggagt aaatggcttt gaaggatatc
      721 acgctggggc agtacttccc cggcaattcc atcgtccacc gcctggaccc ccgcaccaag
      781 ctgatcgcgg tggtgctgta tatcgtggcc ctgttcctgg ccaagtcctt tgtcacttac
      841 ggcatcatgt tcctgctgct ggccgtgtcc atcgccgtct ccaaggtgcc ggtgaagtcc
      901 attttccggg gcatgaagcc ggtggtgttc atcgtcatct tcaccgccat cctcaacctg
      961 ttctacacgc cgggggacac ggtgctggtc cgcttctgga tcttcaccat cacgctggag
     1021 ggcgtgttca acgctttctt catggtggtc cgcatcctga tgctcattgc cggcaccttc
     1081 ctgctgacct acaccacctc ccccatcctg ctcaccgacg ggctggagaa cctgctgggc
     1141 ccgctgaaaa agatccatgt gccggtccat gagctggcca tgatgatgtc catcgccctg
     1201 cgcttcatcc ccctgctgat cgaggagacg gacaagatca tgtccgccca gcgggccagg
     1261 ggggcggatt ttgagtcggg caacctgatc cagcgggcca aggcgctggt ccccctgctt
     1321 gtccccctgt tcatctccgc cttccgccgg gcagacgagc tggccatcgc catggagtgc
     1381 cgctgctacc acggcggcga ggggcgcacc cgcctgcgtc agctgaaata ccggcgcaat
     1441 gacgcggccg tcatcctgct cttcctggtc ctcacggcgg gggtgggggt gctgggccgc
     1501 ttcgggctgt gagccctcac tcctccagca tcagcccggc ggccagctcc cgccgcagcc
     1561 ccagcccgaa gaggtagcag gcgtcgagct gctgggccat atagtcgttc agcttctcct
     1621 caaattcgtt atagaggtca taccccagcc ggtccgccac ctgccgggcg atctggagat
     1681 attcccgctc cgccctgaca taatcgggga cgccgttgca aaatttgtaa atttcatttg
     1741 acaaaagcgg attatcctga aatactgcgt ataacagcgt attcatggtt cattccttcc
     1801 tcacatttta cacggtataa tcgtgtttat tatagcacgg ttttaccgcg tgtcaactat
     1861 gtatttagga gttattatgg agaatttttc tgcccgcctg agacttcttc ggaaagaacg
     1921 cggcttgaag cagcgggaaa tggcggagat ctgtggtttg aagctgcgca gctatcagga
     1981 ctatgagtat caggtgtctt acccttccgc actgggcctg gtggccctgg cggacttctt
     2041 cggcgtcagc ctggactatc tcatgggccg caccgacaag cgggagatca accgctgacc
     2101 gcccccaaaa aagacaagat ccgcccccct ttggtgataa aatggaacga aacattgctt
     2161 taaaactgat gtatgtgggc accgcctacc acggctggca ggtccagaaa aacgccgtca
     2221 ccgtggccga gacgctggag cgggccctgt ccaccgtggt gtgccacccg gtcaaatgca
     2281 ccggcgccgg gcgcaccgac gccggcgtcc acgcccaggt ctatgtggcc aacttccgca
     2341 ccacctccac catcccctgc gaccgcctgc ccctggcggt caacacccgc ctgcccgacg
     2401 acatcgtggt ggtccgggcc gtccaggtgc ccatggaatt caacgccatc ggctcctgcc
     2461 ggaaaaagga atacacctac cgcatttata actcccgcct gggcaacgct ttctatgtgg
     2521 accgggcctg gttctacccc aagcatctgg acgagtcggt gatgcagcgg gccgccagcc
     2581 agttcgtggg cacccacgac tttcgggcgg tgcgctccgt gggcaccgag acccgcacca
     2641 ccgtgcgcac cgtccactac ttccaggtgg agcggaaggg ggagctgatc gagtgccggg
     2701 tgtgcgccga cggcttcctc tacaacatgg tccgggccat ggtgggcact gtggtctatg
     2761 ccgccgaggg caagctggcc ccggaggaca tccccgccat cctggactcg ggcaaccgca
     2821 ccctcgccgg ccccaccgtc ccacccggcg ggctgtatat gaccaggctg tggtatgacg
     2881 aggacgttgt gtaagggggc ctggcgcccc ctcccgcccg gggggagaga ggtaacatga
     2941 aggatcagac aacaaagcag gcatccgcct cccaaaaacc gaatattctg gtccggctgc
     3001 tggctctgct ggtcaccgcc gctttggccc tgggagccct gaccctggtg gtctaccggg
     3061 accagttcaa cctggacgcg gtgaagcgct ggctcgccct gcgctctgtg gagaccgacg
     3121 cctccggcgc cgccgccccc ttcacccacg cgggcgggga cagcatctcc ttcgcctatc
     3181 tgaagggggg catccttcag tgctccgccg ccggcgccca ctactactcc ttctccggcg
     3241 accagtacgc tgaggaggtc acccccctca gccagcccgt cctgtccgcc tcctcctccg
     3301 caggcgtggt ctatgacgcc ggcggagaga gcctgtttct ctttcaggac ggtcaggagg
     3361 ccttttccct ggacgtggac ggcaccctcc tctccgcccg ggtcaacgac agcggctggc
     3421 tggctgttac cgcccagcag agcggctata agggctcggt cacggtgtat aacgtcaaca
     3481 gcaccagccg gaaggagatc atccagctga acttttccag cgtgttcgtg gccgacgccg
     3541 ctgtctctcc ggacagcaag acggtggccg tgatcaccat cgggcaggag aacgggtcct
     3601 ttttcagtca gctcctcctc tatccggtgg acagcagtga gccctccgcc accctctccc
     3661 tgggcaacct ggcggtgctg gacctggact atgaggcgga gcagct
//
LOCUS       DBMDSJ010000140         3693 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6396_length_3693_cov_1.448775, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000140 DBMDSJ010000000
VERSION     DBMDSJ010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6396_length_3693_cov_1.448775"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /gene="xseB"
                     /locus_tag="ACZPAQ_04480"
     CDS             <1..277
                     /gene="xseB"
                     /locus_tag="ACZPAQ_04480"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR0903274.1"
                     /translation="RQPGVPGGGKEVPVMAGKKLDFEQSMARLEEIVGLLERGDAPLD
                     EAMALFEEGAKLMLECTKRLDQAEQKVTLLTAGEDGQPREEPFGGGL"
     gene            279..1163
                     /locus_tag="ACZPAQ_04485"
     CDS             279..1163
                     /locus_tag="ACZPAQ_04485"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR0903275.1"
                     /translation="MSLEFERRLAGDQKRIEEALGAIFREDDRYADLQEAMEYSLMAG
                     GKRVRPVLTLECCRLCGGDPERALPFACGVEMVHTYSLIHDDLPCMDDDDLRRGRPTN
                     HRVYGEATAVLAGDGLLTAAFQMLTARRDLLSPEQMAEGVDCLSRAAGPSGMVGGQVL
                     DMAGEGRSLSLSELELLQSLKTGALISAAAELGCIAAGGSPEQRRAVARYAQKLGRAF
                     QIRDDMLDVTGTTEEFGKPVGSDRSREKSTFVTALGLEGSGALVEQLTEEAVSALNGF
                     PQPDFLIWLARELASRRK"
     gene            1195..1659
                     /locus_tag="ACZPAQ_04490"
     CDS             1195..1659
                     /locus_tag="ACZPAQ_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR0903276.1"
                     /translation="MFEGRTGFLTGNLVLDLSILAWFLAQLIKTVANLVVNRNLDLKR
                     MVGSGDMPSSHSSFICAATASIGQIYGWQAPLFSLAAVVALVVMYDACNVRRAAGEQA
                     KVLNYVMAHWKEMPEELKPKKKLNEHLGHTLPQVLMGALLGTAIGITGPMIW"
     gene            1711..3585
                     /gene="dxs"
                     /locus_tag="ACZPAQ_04495"
     CDS             1711..3585
                     /gene="dxs"
                     /locus_tag="ACZPAQ_04495"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR0903277.1"
                     /translation="MDKRDKTTSYGPILAGMGDQEARELCGQLRQELIRDVARTGGHL
                     SSNLGVVELTVAIHRVFDTSRDRLVFDVGHQSYPHKMLTGRREQMSTIRQLGGIAGFP
                     KPSESVHDAFIAGHASNSVAVAVGMARARDMLGEDYRVLALIGDGALTGGLAYEGLST
                     AGQSRGQLLVILNDNGMSITKNVGGVAEHLARQRLKPQYLTFKKYYRKVMNATWFGRK
                     IYRVTHRLKKAVKESLLPSSLFENMGFTYLGPVNGHDLRELTEILRYARDLKEPVLLH
                     VRTIKGKDFTPAEENPDAYHGVSPFDPETGEPLKQGRASFSSVFGREVARLAREDPRV
                     CAVTAAMQTGTGLDYFALQFPDRFFDVGIAEGCAVSMAAGMAKQGAVPVVAVYSTFLQ
                     RAYDMLLHDVALDRLHVVLAVDRAGLVGEDGETHQGVFDVSYLNSVPGMRVYAPANFA
                     ELKAMLEEAVLRWDGPAAVRYPRGGEGEYREDSGRAPAAVLRRGSHITLVSYGVEIND
                     VLQAARLLEERGVEAEVVKLNIITPLDAAPVLDSVRRTGALLVAEDSVSAGSVGERLA
                     CALEQAGISARVALCSSGDTFTPHGSVGELKRLLELDGAGLAQRAMEVLRFDKTAAGC
                     "
     gene            3560..>3693
                     /locus_tag="ACZPAQ_04500"
     CDS             3560..>3693
                     /locus_tag="ACZPAQ_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR0903278.1"
                     /translation="MTKQRLDVELTARGLAESRQKAQAMIMAGQVYVDGRKVDKAGTQ
                     V"
BASE COUNT          639 a         1081 c         1387 g          586 t
ORIGIN      
        1 acggcagcct ggcgtgccgg gtggaggaaa ggaagtgcct gtgatggcgg gaaaaaagct
       61 ggattttgag cagtccatgg cccggctgga ggagatcgtg ggcctgctgg agcgggggga
      121 cgccccgctg gatgaggcca tggcgctctt tgaagagggg gcgaagctga tgctggagtg
      181 caccaagcgc ctggatcagg cggagcagaa ggtgaccctg ctcaccgcag gggaggacgg
      241 acagccccgg gaggagccct ttggaggagg actgtgatat gagcctggaa tttgagcgcc
      301 ggctggccgg cgaccagaag cggatcgagg aggccctggg ggccatattc cgggaggacg
      361 accggtacgc ggacctccag gaggccatgg agtacagcct gatggcgggg ggaaagcggg
      421 tccggcccgt gctgaccctg gagtgctgcc gcctgtgcgg cggcgacccg gagcgggccc
      481 tgccctttgc ctgcggggtg gagatggtgc atacctactc cctgatccat gacgacctgc
      541 cctgcatgga cgacgacgac ctgcgccggg ggcgccccac caaccacagg gtgtatgggg
      601 aggccaccgc ggtgctggcc ggggacggcc tgctcaccgc ggcctttcag atgctgactg
      661 cccggcggga tctgctctcc ccggagcaga tggcagaggg ggtggactgc ctctcccggg
      721 cggccggtcc ctccggcatg gtggggggac aggtgctgga tatggcgggg gaggggcgga
      781 gcctgtccct gagtgagctg gagctgctcc agagcctgaa gaccggcgcc ctgatctccg
      841 ccgcggcgga gctggggtgt attgccgcgg gggggagccc ggagcagcgg cgggccgtgg
      901 cccgctatgc tcagaagctg ggccgggcct tccagattcg ggacgacatg ctggacgtga
      961 cgggcaccac cgaggagttt ggcaagccgg tgggctcgga ccgctccaga gagaagagca
     1021 ccttcgtcac ggccctgggg ctggagggga gtggcgcgct ggtggagcag ctgacggagg
     1081 aggcagtgtc cgcactgaac ggattcccgc agccggactt cctcatctgg ctggcccggg
     1141 agctggcctc ccggcggaag tgaaaacaga cgggacagaa gggagcgaag gcaaatgttt
     1201 gaggggcgta ccggtttttt aacggggaat ctggtgctgg atctgtccat cctggcgtgg
     1261 ttcctggccc agctgatcaa gacagtggcc aatctggtcg tgaaccggaa cttggacctg
     1321 aaacggatgg taggcagcgg cgacatgccc agctcccact cctccttcat ctgcgcggct
     1381 acggcgtcca tcgggcagat ctacggctgg caggcgcccc tgttctccct ggcggcggtg
     1441 gtggccctgg tggtgatgta cgacgcctgt aatgtgcgcc gggccgctgg ggagcaggcc
     1501 aaggtgctca actatgtgat ggcccactgg aaggagatgc cggaggagct caagcccaaa
     1561 aagaagctga atgagcacct gggccacacg ctcccccagg tgctgatggg ggctctgctg
     1621 ggcacggcca ttggcatcac cggacccatg atctggtaaa ccgggagggg aggcgctgcg
     1681 gcgccgccca ggaagctgtg agggattttt ttggataaga gagacaaaac aacctcatat
     1741 ggccccatcc tggcggggat gggggaccag gaggcccggg agctgtgcgg ccagctccgg
     1801 caggagctga tccgggacgt agcccggacc ggagggcatc tgtcctccaa cctgggggta
     1861 gtggagctca ccgtggccat ccaccgggtg ttcgacacca gccgggaccg gctggtcttt
     1921 gacgtgggcc atcagagcta cccacacaag atgctcacag gccggcggga gcagatgtcc
     1981 accatccggc agctcggcgg gatcgccggc ttccccaagc catctgagag cgtccatgac
     2041 gcattcatcg ccggacacgc ctccaactcg gtggcggtgg cggtggggat ggcccgggcc
     2101 agggacatgc tgggggagga ctaccgggtg ctggctctca tcggagacgg agcgctcacc
     2161 ggcggactgg cctatgaggg cctgtccacc gcggggcaga gcaggggcca gctgctggtc
     2221 atcctcaacg acaacggcat gtccatcacc aagaacgtgg gcggcgtggc ggagcacctg
     2281 gcccgccagc gcctgaagcc ccagtacctc accttcaaga agtactaccg gaaggtgatg
     2341 aacgccacct ggttcgggcg gaagatctac cgggtgaccc accggctgaa gaaagcggtg
     2401 aaggagagcc tgctccccag cagcctgttt gagaatatgg gcttcaccta tctggggccg
     2461 gtgaacggcc acgacctgcg ggagctgacg gagatcctcc gctacgcccg ggacctgaag
     2521 gagccggtcc tgctccatgt caggaccata aagggaaaag acttcacacc tgctgaggag
     2581 aatcccgacg cctaccacgg cgtgtccccc tttgatccag agacagggga gcccctgaag
     2641 caggggcggg ccagcttctc ctccgtgttt gggcgggagg tggcccggct ggcccgggag
     2701 gacccgcggg tctgcgccgt cacggcggct atgcagaccg ggactgggct ggattacttt
     2761 gccctccagt tcccggaccg ctttttcgat gtgggcattg ccgaaggctg cgcggtgtcc
     2821 atggccgccg gcatggccaa gcagggggcg gtcccggtgg tggcggtcta ttccaccttc
     2881 ctccagcggg cctatgacat gctgctccac gacgtggctc tggaccggct gcatgtggtg
     2941 ctggcagtgg accgggccgg gctggttggg gaggatggcg agacccacca gggggtcttt
     3001 gacgtgtcct atctcaacag cgtccctggg atgcgggtct atgcccccgc caattttgcc
     3061 gagctgaagg caatgctgga ggaggcggtc ctgcgctggg acgggccggc ggcggtgcgc
     3121 tatccccggg gcggcgaggg ggagtaccgg gaggacagcg gccgggcccc ggcggccgtc
     3181 ctgcgacggg ggtcccacat cacgctggtg tcctacggtg tggagatcaa cgatgtgctc
     3241 caggcggccc ggctgctgga ggagcggggc gtagaggcgg aagttgtaaa gctgaacatc
     3301 attacaccgc tggatgccgc tccggtgctg gactctgtgc gccggacggg cgccctgctg
     3361 gtggcggagg acagcgtctc cgccggcagc gtgggggagc ggctcgcctg cgcgctggag
     3421 caggccggca tcagcgcccg ggtggcgctg tgcagctccg gcgatacctt taccccccac
     3481 ggcagcgtgg gagaattgaa gcggctcctg gaactggacg gagccgggct ggcccagcgg
     3541 gccatggagg tgctgcgatt tgacaaaaca gcggctggat gttgaactga ctgcccgggg
     3601 actggcggag agccgccaga aggcccaggc catgatcatg gcggggcagg tctatgtgga
     3661 cggacggaag gtggacaagg cgggcaccca ggt
//
LOCUS       DBMDSJ010000141         3684 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6410_length_3684_cov_1.660340, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000141 DBMDSJ010000000
VERSION     DBMDSJ010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6410_length_3684_cov_1.660340"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..954
                     /gene="sstT"
                     /locus_tag="ACZPAQ_04505"
                     /pseudo
     CDS             1..954
                     /gene="sstT"
                     /locus_tag="ACZPAQ_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411309.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            complement(1007..1825)
                     /locus_tag="ACZPAQ_04510"
     CDS             complement(1007..1825)
                     /locus_tag="ACZPAQ_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR0903279.1"
                     /translation="MSKFFSLAYLTLPNTHPVDQIEIAAQCGYEGVGLRTISQHLPGE
                     PDYALANGRMFQMVRAALERTGVRLMDIELARVADGVDVMSYEKEFALGAELGAKYAI
                     ASVWSADRDFSRVQFEKMCDLADKYGMKLNLEFVPFSNIPGLTEALAWMDLMDRPNVQ
                     VLVDTLHAHRVKVTPEQLREVAAERFGFVHLCDGPAFIPPVDHPDMVGVARGGRLYVG
                     EGGIDLAGMLKNIPDPPYYSIELPNAAEVAIRGKLEHARRCLETAKTFFAAHQI"
     gene            complement(1989..3107)
                     /locus_tag="ACZPAQ_04515"
     CDS             complement(1989..3107)
                     /locus_tag="ACZPAQ_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR0903280.1"
                     /translation="MKKAIALILTLAMSLSLAACSGGGSGSGAASNPPAASGGSSTSG
                     TVSSGSASGAYADLEPVELSLADAAAVGAAGETFDRLFAEKVEEITGGKLTVYLYQNG
                     ELGSDMDILSQMQDGHLDLVGSQIAAVVNFVPEMAIFDLPMVFAQYDGETIDQVLNGD
                     SQTHAAMSAAYETAGLELLGIQQYATYRLTTANRDLKTLADFKGLQIRTMENGNHMAF
                     WTAIGAAPTPLAWAEVFIALQNGTIDAHENAADTVLGANIQEVQKYLACTNHILYANQ
                     LSMNKEKYDSLDPAYQAAIVQAVEEAIEEMKPEMKNIDETSKQALVDGGMTLIEYDDS
                     FYQEILALDTVQALYDKIDADVNGLGTTLQEELAAAQG"
     gene            complement(3186..>3684)
                     /locus_tag="ACZPAQ_04520"
     CDS             complement(3186..>3684)
                     /locus_tag="ACZPAQ_04520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR0903281.1"
                     /translation="IDTLVATAGIIRHCPIDEMTEAQWQDVVDINLTGVFHAVKSVVP
                     SMKERKYGHIVVISSIGGRTGRPGVGVNYAATKAGVNGLVMCLGYELGPWQITVNSVA
                     PGPLKGKMFAGMTQDRIESLSAGIRLQRMGELSDVAAAIAYLGSDDAAWTTGEVLDVN
                     GGLQY"
BASE COUNT          646 a         1201 c         1081 g          756 t
ORIGIN      
        1 cgctgtgtgc ggtggttgcc agctttctgt ttccctccac cctggcgctg gcccaggagg
       61 cggcctccag cgcccccggc ggcatcggcg agatcctcca gaccctgctc aacaactcgg
      121 tgagcaaccc catctccgct atggcggagg ccaactatat cggggtgctg ctgtgggctg
      181 tgggctgtgg tcttcggcat cgccctgcgg gacgcctccg accgcaccaa gcaggtgctg
      241 ggcgacctgt ctgacgccct ctccaccgcc atccgctggg tcatcagctg cgcccccttc
      301 ggcatcctgg gtctggtgtt caccacggta tctgagaccg gcttctcctc cttcatggac
      361 tacggccgcc tgctctgcgt cctggcgggc tgtatgttct tcgtggcctt gatcgtcaac
      421 cccattctgg tcttcctgtg catccggcgc aacccctatc ccctggtgtt ccgctgcctg
      481 aaggagagcg gcatcatggc cttcttcacc cgcagctccg ccgctaatat cccggtcaac
      541 ctgcggctgt gcaaggagct gggcctggac gaggagaagt actccgtctc catccccttg
      601 ggagccacca tcaacatggc tggggccgcc atcaccatct cggtcctcac cctggccgct
      661 gccaacaccc tgggcatctc ggtggacttt cctaccgcct tcctgctcag cgtggtagcc
      721 tccatcgccg cctgcggctc ctccggtgtg gcggggggct ccctgctgct gattcccatg
      781 gcgtgcagcc tgttcggcat tcccaacgac atcgccatgc aggtggtggg catcggcttc
      841 atcatcggcg tcgtccagga ctcctgcgag acggcactga actcctcctc cgacgtgctg
      901 ttcaccgcgg ccgccgagtt ccgcagccgc cgcctggccg ctcgtcagga ctgaccaaac
      961 gccaaaaaga ggcccgggat cagaagatcc cgggcctctt ttctcttcag atctggtgcg
     1021 ccgcgaagaa ggttttggcg gtctccaggc agcgcctggc gtgctccagc ttgccccgga
     1081 tcgccacctc cgccgcgttg ggcagctcga tggaatagta ggggggatca gggatgttct
     1141 tcagcatccc cgccaggtcg atgccgccct ctcccacgta cagccgcccg ccccgggcca
     1201 cgcccaccat gtccgggtgg tccaccggcg ggatgaaggc cggaccgtcg cacaggtgga
     1261 cgaagccgaa tcgctccgcc gctacctccc gcagctgttc tggggtgacc ttcacccggt
     1321 gggcgtgcag agtgtccacc agcacctgca cattgggccg gtccatcagg tccatccagg
     1381 ccagggcttc ggtcaggccg gggatgtttg agaaggggac gaactccaga ttgagcttca
     1441 tgccgtattt gtccgccaga tcgcacatct tctcgaactg cacccgggag aagtcccggt
     1501 ccgcactcca cacgctggcg atagcgtatt tcgcccccaa ctcggccccc agggcgaact
     1561 ccttctcgta gctcattacg tccaccccgt ccgccacccg ggccagttcg atgtccatca
     1621 gcctcacgcc ggtgcgctcc agggcagcgc ggaccatctg gaacattcgg ccgttggcca
     1681 gggcgtagtc cggctctccg ggcaggtgct gggagatggt ccgcaggccc accccctcat
     1741 agccgcactg ggcggcgatc tcgatctgat ccaccgggtg ggtgttgggc agagtcagat
     1801 aggccaggga aaaaaacttg ctcataagag agacctcctt attctgtggg gcgttcgccc
     1861 gtctgtttga ttgctggaag gggaatgccc ccttacggcc cgctctgacc agcggaccgc
     1921 aaagaggcat tcggtttaag aggagcaaca ggaagaaact gctgctaagc ggaatatcgc
     1981 gggatagatc agccctgagc ggcggcaagc tcctcctgaa gggtggtgcc cagaccattt
     2041 acatctgcat cgatcttatc gtagagggcc tggacagtat ccagcgccag gatctcctga
     2101 tagaaagaat catcatattc gatgagagtc atacccccgt ccaccaaagc ctgcttgctg
     2161 gtctcatcaa tattcttcat ttcaggtttc atttcctcaa tggcttcctc caccgcctgc
     2221 acgatagccg cctgataggc ggggtccaga gagtcatact tctccttgtt catggacagc
     2281 tggttggcgt acaggatgtg gttggtgcag gccaggtact tctgcacctc ctgaatattg
     2341 gccccgagaa cagtatccgc cgcattttcg tgggcatcga tggtcccgtt ctgcagggca
     2401 ataaacacct cagcccaggc cagaggggtg ggggcggcac cgatggcagt ccagaaggcc
     2461 atgtggttgc cgttctccat ggtgcggatc tgaagcccct taaagtctgc caaggtcttc
     2521 aagtcccggt tggcggtggt gaggcggtag gtggcgtact gctggatgcc cagcagttcc
     2581 agaccggcgg tctcataggc ggcggacatg gcggcatggg tctggctgtc gccgttgagc
     2641 acctggtcga tggtctcgcc gtcatactgg gcaaacacca tgggcaggtc gaagatagcc
     2701 atctcaggga caaagtttac gaccgctgcg atctgggagc ccactaaatc aaggtgtcca
     2761 tcctgcatct gggaaagtat gtccatgtca ctgcccagtt ctccattctg atagagatag
     2821 actgtcagtt ttccgccagt gatctcctcc actttctccg caaacaggcg gtcgaaagtc
     2881 tctccagccg cccccaccgc ggcagcatct gccagagaca gctccacagg ctccagatcc
     2941 gcataggccc cggaagcaga accggaagag accgtaccag atgtgctgct accgccggaa
     3001 gcagccgggg gattggaagc ggctccggag ccgcttccac cgcctgagca ggcggccagg
     3061 ctcagggaca tggccagggt cagaatcagg gcaatcgctt ttttcattgt ctttcctcct
     3121 cgttttatgt atcagctatg gatcagcgcc gttttcacgg cttttgcaca aaggatctgc
     3181 cggcctcaat actggaggcc gccgttcaca tccagcacct cgccggtggt ccaggcggcg
     3241 tcgtcgctgc ccagataggc gatggcggcg gccacgtcgc tcagttcgcc catcctctgg
     3301 aggcggatac cggcggacag ggattcgata cggtcctggg tcatgccggc gaacatcttt
     3361 cccttcaggg ggccgggggc cacgctgttc accgtgatct gccagggccc cagctcatag
     3421 cccaggcaca tcaccaaccc gttcacaccg gccttggtgg cagcgtagtt gacacccacg
     3481 ccgggacggc ccgtccgacc tccaatggag gagatgacca cgatatgtcc gtatttccgc
     3541 tccttcatgg aggggaccac cgacttcacc gcgtggaaca cgccggtgag gttgatgtcc
     3601 acaacatcct gccactgggc ttcagtcatc tcgtcgatag gacagtggcg gatgatgccg
     3661 gcggtggcta ccagggtgtc gatc
//
LOCUS       DBMDSJ010000142         3666 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6452_length_3666_cov_1.433100, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000142 DBMDSJ010000000
VERSION     DBMDSJ010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_3666_cov_1.433100"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /gene="ispH"
                     /locus_tag="ACZPAQ_04525"
     CDS             complement(<1..753)
                     /gene="ispH"
                     /locus_tag="ACZPAQ_04525"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR0903282.1"
                     /translation="MKIRIANSAGFCYGVRRAVELAEGAAQSGEPCVMLGPIIHNQDM
                     MDHLARHGLRAVVRPEEVPEGSEVIIRSHGESRAVHEGLHARGVRILDATCPNVTRIH
                     QIVARAEEQGRQPIIIGTRDHPEVLAIAGWCCRPVVLSGAEELENWLAEDPSRRQMPL
                     TFVSQTTAIRRVWDSCVKKAKKECTNAEFFDTICGATSKRQEEAVQLAAQCGAMVVIG
                     DRQSSNTRKLAELCREVCPSVQLIERVDDLELS"
     gene            complement(750..1349)
                     /locus_tag="ACZPAQ_04530"
     CDS             complement(750..1349)
                     /locus_tag="ACZPAQ_04530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR0903283.1"
                     /translation="MSTEQFYRFLYLVVWPFFNLFHPVRTVGREHIPEGAAVICPNHT
                     ALSDPFFVAFAFQKKNPLRVMAKIQLMRVPVIGWLLGKAGIFGVDRGHADMHAAKTAL
                     RCLKEGHKLLIFPEGTRVEEGENVDAKAGAAMFATRTGVPIVPMYIPAQKHWFRPTTV
                     VIGEPYYPQIQGRRATAEENQEIAREIMRRIHALGGLPQ"
     gene            complement(1346..2023)
                     /gene="cmk"
                     /locus_tag="ACZPAQ_04535"
     CDS             complement(1346..2023)
                     /gene="cmk"
                     /locus_tag="ACZPAQ_04535"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR0903284.1"
                     /translation="MQEKSVAIDGPAGAGKSTLARALARELGYLYVDTGAIYRTVALR
                     AWEAGADPSDPEQIAPLLKGLDLRMDYGADGVQRMYLSGRDVTEAIRVHQISGLASQV
                     AALPPVRDFLLDFQRRQAMEHDVVMDGRDIGTVVLPHAGAKVFLTAAPEARARRRLLE
                     LKQRGQETELETVLRDIVQRDEQDRNRAVAPLRQAEDAVLLDTTELGLEESLKALISL
                     VKGRLAQ"
     gene            complement(2076..3341)
                     /locus_tag="ACZPAQ_04540"
     CDS             complement(2076..3341)
                     /locus_tag="ACZPAQ_04540"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR0903285.1"
                     /translation="MADELIVVGGGAAGMMAAISAAETGAAVRLLERNPKVGRKLYIT
                     GKGRCNVTNHCPPEEVLASTPRNGRFLYGAVTRTPPAWVEDFFTGLGVPLKVERGNRV
                     FPVSDRAADVIDALFFHMKKLNIPVTQARASQICLEEGRVSGVETDCGFFPCRAAVLA
                     TGGCSYPATGSTGDGYEMARALGHTVVSPVPSLVPLEAKEDFCGKMQGLALKNVSLRV
                     KNQKGKVVYQEQGELLFTHFGLSGPLVLSASAHMRDFAREQYTCVIDLKPALEEQKLD
                     ARLVRELSEQANRDFQNVLGSLVPRLMVPTVIALTGIPGDRKAHDMTKGERRRLLEVL
                     KGLVIHIRGPRPIAEAIITSGGVKVGEIDPKTMGSKKVPGLFFAGELIDVDAYTGGFN
                     LQIAWATGRAAGESAARTCLGQDLGGAGA"
     gene            complement(3345..>3666)
                     /locus_tag="ACZPAQ_04545"
     CDS             complement(3345..>3666)
                     /locus_tag="ACZPAQ_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR0903286.1"
                     /translation="EDVVIGVSFRRYSSRTVQAMNFARDRGATTIAITDSEASPLAPI
                     SNYTLKARSDMASFVDSLVAPLSLVNALLVAVSRKKNDDLAHTFQTLEDIWDEYGVYE
                     KVQD"
BASE COUNT          616 a         1267 c         1113 g          670 t
ORIGIN      
        1 agacaactcc aggtcatcta cccgctcgat cagctggaca gatggacaga cttcccggca
       61 gagctccgcc agcttcctgg tattggagct ctggcggtcg ccgatgacca ccatcgcgcc
      121 gcactgggcg gccagctgga cggcctcttc ctggcgtttt gatgtcgctc cacatattgt
      181 atcaaaaaat tctgcgtttg tacactcttt ttttgctttt ttcacgcagg aatcccaaac
      241 cctgcggatg gccgtggtct gggacacaaa agtgagtggc atttggcgtc gggacgggtc
      301 ctccgccagc cagttttcca gctcctccgc tccagacagg accaccgggc ggcagcacca
      361 gcccgcaatg gccagcacct ccgggtggtc ccgggttccg atgatgatgg gctgcctccc
      421 ctgctcctca gcccgggcca cgatctgatg gatacgggta acattgggac aggtggcgtc
      481 caggatgcgg acgccccggg catgcaggcc ctcgtggaca gcgcggctct ccccatggga
      541 gcggatgatg acctcgctcc cctccgggac ctcctccggc ctgaccaccg cccgcaggcc
      601 atggcgggcc agatggtcca tcatgtcctg gttgtggatg atgggaccca gcatcacaca
      661 gggctcgccg gactgggcgg ccccctccgc cagctccacc gcccggcgca cgccgtagca
      721 gaagccggcc gaatttgcga tccttatctt cactggggaa gtccccccag tgcgtggatg
      781 cgccgcatga tctcacgggc gatctcctga ttttcctccg ccgtggcccg ccgcccctgg
      841 atctggggat agtaaggctc gccgatcacc acagtggtag ggcggaacca gtgcttctgc
      901 gccgggatgt acatggggac gatgggcacc cccgtccggg tggcaaacat ggccgctccc
      961 gccttggcgt ccacgttctc cccctcctcc acgcgggtcc cctcggggaa gatgagcagc
     1021 ttgtgcccct ctttcaggca gcgcagagcc gtcttggccg cgtgcatgtc cgcatggccc
     1081 cggtccactc cgaagatgcc cgccttgccc agcagccagc cgatcacggg cacccgcatc
     1141 agctggatct tggccatcac ccgcagaggg ttctttttct gaaaggcgaa agccacaaaa
     1201 aacgggtcgc tgagggcggt atggttgggg cagatcaccg ccgccccctc tgggatgtgc
     1261 tcccgcccca cggtgcgtac cggatggaac agattgaaaa agggccagac gacgagatag
     1321 aggaatcggt agaactgttc tgtactcatt gagccagcct ccctttcacc agggagatca
     1381 gggccttcag gctctcctcc agtcccagct ccgtggtatc caacagcaca gcgtcctccg
     1441 cctgacggag aggggccacc gcccggttcc ggtcctgttc atcccgctgg acgatgtccc
     1501 ggagaacggt ctccagctcc gtctcctgtc cccgctgctt cagttccagc aggcggcggc
     1561 gggcccgggc ctccggcgcg gcggtaagga agacctttgc ccccgcgtga gggagcacca
     1621 ccgtcccgat gtcccggccg tccatgacca cgtcgtgctc catggcctga cggcgctgaa
     1681 agtccagcag gaagtcccgc accgggggca gggcagccac ctgggaggcc agtccggaga
     1741 tctggtggac ccggatagcc tctgtcacgt cacgtccaga caagtacatc cgctgtacac
     1801 catctgcccc ataatccatg cgcaggtcca gccccttcag cagaggggcg atctgctccg
     1861 ggtcggatgg atccgcccct gcctcccagg cccgcagggc caccgtgcgg tagatggccc
     1921 ccgtgtctac atacaaatac cccagctccc gggccagggc ccgggccagg gtgctctttc
     1981 cggccccggc ggggccgtcg atggcaacgc ttttttcctg catttcttat ttcgctccaa
     2041 aatttgattg attctccgct ccggtccgac ggtactcagg cccctgctcc ccccaggtcc
     2101 tgccccaggc aggtccgggc ggcgctttcc cctgccgccc ggccggtggc ccaggcgatc
     2161 tggaggttga agccccctgt ataggcatcc acgtcgatca gctccccggc gaagaacagg
     2221 ccgggcacct ttttcgaccc catggtcttg gggtcgatct cccccacctt caccccgccg
     2281 gaggtgatga tggcctccgc aatgggccgg ggaccccgga tatggatcac aaggcccttc
     2341 aggacctcca gcaggcggcg gcgctccccc ttggtcatgt cgtgggcctt tcggtccccg
     2401 gggatgccgg tgagggcgat gacggtgggc accatcagcc tgggcactaa actgcccagg
     2461 acgttctgaa agtcccggtt ggcctgctcc gagagctccc ggaccagccg ggcgtccaac
     2521 ttctgttcct ccagagccgg cttcaggtcg atgacacagg tgtactgctc cctggcaaag
     2581 tcccgcatat gggcgctggc gctgagcacc aggggaccgg agaggccaaa atgggtgaac
     2641 agcagctccc cttgctcctg atagaccacc tttcccttct ggttcttgac ccggagggac
     2701 acatttttca gcgccaggcc ctgcattttt ccgcagaagt cctcctttgc ctccaagggg
     2761 accagggatg gaacgggcga gaccacagta tggcccaagg cccgggccat ctcatatccg
     2821 tcgccggtag agccggtggc cggataggag cagccgccag tggccaggac cgcagcccgg
     2881 cagggaaaga agccgcaatc ggtctccacc ccggacaccc gtccctcctc cagacagatc
     2941 tgggaggccc gggcctgcgt caccgggata ttcagctttt tcatatgaaa aaacagggcg
     3001 tcgatgacat ccgccgcccg gtccgacacc ggaaagaccc ggttgccccg ctctaccttc
     3061 agcgggaccc ccaggccggt gaaaaagtcc tccacccacg ccggcggcgt acgggtcacg
     3121 gccccgtaga ggaaccggcc gttccggggc gtgctggcca gcacctcctc cggcggacag
     3181 tggttggtca cgttgcaccg gcccttgccg gtgatgtaca gcttgcgccc taccttgggg
     3241 ttgcgctcca gcagccggac ggccgctccc gtctccgcag cggaaatggc ggccatcatg
     3301 ccggcggccc cgccgccgac cacgatcagt tcatcggcca tcgttcagtc ctgcaccttc
     3361 tcatagaccc cgtactcatc ccagatgtcc tccagggtct ggaaggtatg ggccaggtcg
     3421 tcatttttct tccggctcac cgccaccagc agggcgttga ccaggctgag tggggccacc
     3481 agggagtcca cgaaggaggc catatcgctg cgggccttca gtgtgtagtt ggagatgggg
     3541 gccaggggcg aggcctcgct gtccgtgatg gcaatggtgg tggcgccccg gtcccgggcg
     3601 aagttcatgg cctggacggt gcggctggag taccgccgaa agctcacgcc gatgaccacg
     3661 tcctcc
//
LOCUS       DBMDSJ010000143         3650 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6480_length_3650_cov_1.663567, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000143 DBMDSJ010000000
VERSION     DBMDSJ010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6480_length_3650_cov_1.663567"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            78..428
                     /locus_tag="ACZPAQ_04550"
     CDS             78..428
                     /locus_tag="ACZPAQ_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HIR0903287.1"
                     /translation="MQTIDEALEKLQASPFRAKFRLSERERAYIGEKGLDTIREHARG
                     FIETRLAPAELPNDGRQTPMRGHPVFLAQHACACCCRKCLYKWYKIPPGIQLNRRQQE
                     KIVNLLMAWIQRQL"
     gene            465..878
                     /locus_tag="ACZPAQ_04555"
     CDS             465..878
                     /locus_tag="ACZPAQ_04555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR0903288.1"
                     /translation="MTEVVAALIWEGETFLICQRPAHKARGLLWEFVGGKVEPGETRE
                     EALVRECREELDIRVAVGEPFMEVIHAYPDLTVHLTLFHASIAEGTPRKLEHQDIRWI
                     RAAEIPQYRFCPADEAILERLREVAGCGGPTQVGR"
     gene            complement(1041..3161)
                     /gene="vanT"
                     /locus_tag="ACZPAQ_04560"
     CDS             complement(1041..3161)
                     /gene="vanT"
                     /locus_tag="ACZPAQ_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HIR0903289.1"
                     /translation="MSARRDVPVLDRFRLLAAFLAVCNHTAPLAGLAPEADFWLTRVL
                     ARIAVPFFLMVSGYFLSRDGWRGTGRFLKRTLTLYAAAVLLYLPLNWYNGGFSPLEWL
                     KKLLIDGTLYHLWYFPAVLLGVVIARGLARLGLPAALAAALLLYLIGLGGDSYYGLTA
                     QLPALDALYGGLFRLCSYTRNGLFLAPLFLLLGAAGVRLGRGISALGTLLSLAGMSLE
                     AFWLRAQGWPRHDSMYLFLPLTMLFLFSLLLNGGRGQDRTARRLSLLIYLLHPWSIVL
                     VRGAAKLTGLEALLIQNSLAHFCAVAALTLCAALVLDALRPLRPAPGGRAWKELDLDA
                     LRHNARTLRSALSPGCTLMAVVKADGYGHGAVPVARCLRRDGVRAFGVACLAEGIALR
                     KAGVLGTILILGYTPPREVPLLRRWHLTQTVADEAHGRALAAQGGLVRVHLALDTGMH
                     RLGLPAGDRSAIARMYRLKNLRIDGVFSHLCVSDSLKKEDMDATLEQLTLFYDTLDWM
                     RTQNLSPGRVHIQASYGVLNLPPQPCAWARAGIALYGVYSSLSPVQRRLDLKPVLSLR
                     ARVASVRTLRPGERAGYGLAFHARQETRLAVVTIGYADGLPRQLPQQGGRVLIRGAFC
                     PMAGRMCMDQLLVDVTALPGVQAGDVVTLIGRDGARELRAEELAARCGTITNELLSRL
                     GRRLELQKVPRSSQSSGRSAETRG"
     gene            complement(3158..>3650)
                     /locus_tag="ACZPAQ_04565"
     CDS             complement(3158..>3650)
                     /locus_tag="ACZPAQ_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0903290.1"
                     /translation="TRAREGDAFTRQYVALPGCSEHQTGLAIDLGEDVPDLDFIRPSF
                     PDSGACGAFRRAAARYGFLQRYCREKEALTGIAAEPWHFRYVGAPHAQLMEEHGLCLE
                     EYVPFLEVSPRLCRLGNGRTARVSRLPCPSAVLELEPPAGCWQLSGDNCGGFILTVWE
                     GEP"
BASE COUNT          659 a         1168 c         1348 g          475 t
ORIGIN      
        1 gagctgtcct ctccctccaa agggaaagca aggaagtccg atgagccggg ggaggacaca
       61 gagaggagga atgaggggtg cagaccatcg acgaggcact ggagaagctt caggcgtccc
      121 ccttccgggc gaaattccgg ctctcagagc gggagcgggc ctatataggg gagaaggggc
      181 tggacaccat tcgggagcac gcccgggggt tcattgagac ccggctggct ccggcggagc
      241 tgcccaacga cgggcggcag acccccatgc ggggacaccc ggtgttcctc gcccagcacg
      301 cctgtgcctg ctgctgccgc aagtgcctgt acaagtggta taagatcccg ccgggcatcc
      361 agctgaaccg gcggcagcag gagaagatcg tgaatctgct gatggcctgg atccagcggc
      421 agctgtgacc gggctgcgga ggagacagag aggagcgcac agcgatgaca gaagtagtgg
      481 cggccctgat ctgggagggg gagaccttcc tcatctgcca gcgcccggcc cacaaggccc
      541 gggggctgct ttgggagttc gtgggcggaa aggtggagcc gggggagacc cgggaggagg
      601 ccctggtccg ggagtgccgg gaggagctgg acatccgggt ggcggtgggg gagcccttca
      661 tggaggtcat ccacgcctat cccgacctta ccgtccactt gaccctgttt catgcctcca
      721 tcgcggaggg aacgccccga aagctggagc atcaggacat ccggtggatc cgggcggcag
      781 agatcccgca gtatagattc tgccccgcag acgaggccat cctggagcgg ctgcgggaag
      841 tggccggatg cggcggtccc acacaggttg gaagataaga caaggccagc gcctgaatgg
      901 cgctggcctt gatgcgtcgg gagtacggct ccgccatatg acagacgggg tcctcacagg
      961 ggatcagaac tacagaaggg cagggggaca ggacccatgc ccttctcatc ttatcctgtc
     1021 ttgcttccgg tcggggtcgg tcatccccgc gtctctgcgc tccgcccact actctgggaa
     1081 gaacgaggga ccttttgcag ctccagccgc ctgcccagcc gggagagaag ctcattggtg
     1141 atggtgccgc accgggcggc cagctcctcg gcccggagct cccgcgctcc gtcccggccg
     1201 atgagggtga ccacatcccc ggcctggaca ccgggcaggg cggtgacatc caccagcagc
     1261 tggtccatgc acatccgtcc cgccatggga cagaaagcgc cgcggatcag cacacgcccg
     1321 ccctgctggg gcagctggcg gggcaggccg tcggcgtagc cgatggtgac cactgcaagc
     1381 cgggtctcct ggcgggcatg gaaggccagc ccatacccgg cccgctcccc ggggcgcagg
     1441 gtgcgcaccg aggccacccg ggcccggagg gagaggacgg gcttcaggtc caggcggcgc
     1501 tggaccgggg agagggagct gtacacaccg tacagggcga tgccggcccg ggcccaggcg
     1561 catggctggg gcggcagatt gaggaccccg tagctggcct ggatgtggac gcggcccgga
     1621 gagaggtttt gggtgcgcat ccagtccagc gtgtcataga acagggtgag ctgttccaga
     1681 gtcgcgtcca tgtcctcttt tttcaggctg tcggagacgc acagatggga gaacaccccg
     1741 tcgatgcgca gatttttcag acggtacatc cgggcgatgg cggagcggtc cccggcgggc
     1801 agccccagcc ggtgcatgcc ggtatccagg gccaggtgga cccggacgag tcctccctgg
     1861 gcggcaagcg cccggccgtg agcctcgtcg gccaccgtct gggtgagatg ccagcggcgg
     1921 aggaggggga cctcacgggg cggggtgtag cccagaatga ggatggtgcc cagcaccccg
     1981 gccttccgaa gggcgatgcc ctcagccaga caggccaccc cgaaggcccg caccccgtcc
     2041 cgccgcaggc agcgggccac cggcaccgcc ccgtggccgt agccgtccgc tttgaccacc
     2101 gccatcagcg tgcagccggg ggacagggcg gagcggaggg tgcgggcgtt gtgccgcagg
     2161 gcgtccaggt ccagctcctt ccaggcccgg ccgccggggg cggggcggag gggacgcagg
     2221 gcgtccagca ccagggcggc gcacagggtg agcgccgcca cggcgcagaa gtgggccagg
     2281 ctgttctgga tgagcagggc ctccagccct gtcagctttg cagcgccccg caccagcacg
     2341 atgctccagg ggtggaggag gtagatcagc agggacagcc tgcgggcggt gcggtcctgc
     2401 ccccggccgc catttagcag cagggagaac aggaagagca tggtcagggg gaggaaaaga
     2461 tacatgctgt cgtggcgggg ccagccctgg gcgcggagcc agaacgcctc cagggacata
     2521 ccggccaggg agagaagggt gcccagggcg gagatccccc ggcccagccg gactcccgcc
     2581 gcccccagaa gcaggaaaag gggggccagg aacagcccgt tccgggtata ggagcacagg
     2641 cggaacagcc cgccatagag ggcgtccagg gcgggcagct gggcggtcag gccgtagtag
     2701 ctgtccccgc ccaggccgat caggtagagg agcagggcag ccgccagggc ggcgggcagc
     2761 cccagccggg ccaggccgcg ggcgatcacc acccccagca ggaccgccgg gaaataccac
     2821 aggtggtaca gggtgccgtc tatgagcagc tttttgagcc actccagggg ggagaagccc
     2881 ccgttgtacc agttcagggg cagatagagc agaactgcgg cggcgtagag ggtcagcgtc
     2941 cgcttcaaaa atctgcccgt gcccctccag ccgtcccggg agagaaagta cccgctgacc
     3001 atgaggaaaa aagggaccgc gatccgggcc agcacccggg tgagccagaa gtcggcctcc
     3061 ggggccagtc cggccagagg ggcggtgtgg ttgcacaccg ccaggaacgc cgccagcagg
     3121 cggaagcggt ccagcacagg gacatcccgg cgggcgctca tggctctccc tcccagacgg
     3181 tgaggatgaa cccgccgcag ttgtctccgg agagctgcca gcaccccgcc ggcggctcca
     3241 gttccaggac ggcgctgggg caggggagtc gggacacccg ggcggtcctg ccgttgccca
     3301 gccggcagag ccggggggac acctccagga aggggacgta ctcctccaga cagagcccgt
     3361 gctcctccat cagctgggcg tggggcgccc ccacatagcg gaagtgccag ggctcagcgg
     3421 cgatgccggt gagggcctcc ttctcccggc agtaccgctg gaggaagccg taccgggccg
     3481 ctgcccgtcg gaaagcgccg caggccccgg agtcggggaa gctggggcgg atgaagtcaa
     3541 gatcggggac gtcctccccc aggtcaatgg ccaggccggt ctggtgctcg ctgcaccccg
     3601 gaagggccac gtactgccgg gtaaaggcat ccccctcccg ggcccgggtg
//
LOCUS       DBMDSJ010000144         3643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6486_length_3643_cov_1.719650, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000144 DBMDSJ010000000
VERSION     DBMDSJ010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6486_length_3643_cov_1.719650"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="coaBC"
                     /locus_tag="ACZPAQ_04570"
     CDS             complement(<1..1008)
                     /gene="coaBC"
                     /locus_tag="ACZPAQ_04570"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR0903291.1"
                     /translation="MLSGKTILLGVTGGIAAYKAAALASALVKLHASVEVVMTRNATQ
                     FITPLTFEQLTGNRTMVDTFDRNFSHQVEHIALADRTDLVIVAPATANVCAKLAHGLA
                     DDMLTTTVLACPCPKLIAPAMNTKMYENPVTQDNLDLLRRYGWEVIAPASGRLACGAV
                     GPGKMPEPDTLVQHILRVLALPHDLAGRRVLVTAGPTQESLDPVRYLTNHSTGKMGYA
                     IARMAMLRGAEVTLISGPTALEPPPFVTVVPVISAEDMFQAVSRTCQQADFIFKAAAV
                     ADYTPAQYSGDKMKKKDGDLSIPLSRTHDILSYLGEHRRPGQFICGFSMEKPQMNWPG
                     RR"
     gene            1276..2058
                     /locus_tag="ACZPAQ_04575"
     CDS             1276..2058
                     /locus_tag="ACZPAQ_04575"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR0903292.1"
                     /translation="MLIAIDIGNTNIVIGGIQGDAIQFEARIATDRIKTSDQYGVEIK
                     NILALFGVKPCDVDDCIISSVVPPVFNSVYTGLVKMTGKHPLVVGPGIKTGLNIQMDV
                     PSQVGSDRIVIAVAALAEYEPPLILMDLGTATTIEVVDRGSTYLGGCIIPGVRTSLDA
                     LTSRAAQLPGIRLDRPGRVIGKNTVDCMRSGIMYGTAAMLDGMLDRVEEELGSRATVV
                     ATGGMAQFIVPLCRREIKLEKDLLLKGLNIIYQKNAAKRGGR"
     gene            complement(2143..2622)
                     /locus_tag="ACZPAQ_04580"
     CDS             complement(2143..2622)
                     /locus_tag="ACZPAQ_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903293.1"
                     /translation="MDSHMTAVESNKRHKAAGLLAALVLVLILVGWIYYVFSPKELSN
                     VLDFQPSEATDYTVTEMPNAVSSKSRSLDDTPLIEMIDALNIRYCGPSFGVCRFETGQ
                     HIYEIWFFKVNDQLEEIGNISVFENGYIYLGESKYEAVDQERFDELLDFLRSVTTAS"
     gene            2896..3444
                     /locus_tag="ACZPAQ_04585"
     CDS             2896..3444
                     /locus_tag="ACZPAQ_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903294.1"
                     /translation="MTDQELVELFWQRREEALTGLQDKYGGWCRSIAGRILGEERDVE
                     ECLGDCWLQLWNAIPPARPQHFRGYLGAVVRNRALLLRRRRGRQGDTVSLAALELAEC
                     LPSREGPEEQAEAAELGRAISAFLRELGEGPRTAFLRRYWYADTVEETAGHLGWSLSR
                     TKSVLFRTRKKLKAYLEQEGML"
     gene            3441..>3643
                     /locus_tag="ACZPAQ_04590"
     CDS             3441..>3643
                     /locus_tag="ACZPAQ_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903295.1"
                     /translation="MNRGERMMEAVGLVDPALVEQAAGPARRHSARRPLRTGLIAACL
                     CAALLGSAWAANQIGGFRSLKFF"
BASE COUNT          720 a         1014 c         1220 g          689 t
ORIGIN      
        1 ccgccgccct ggccagttca tctgcggctt ctccatggag aagccgcaga tgaactggcc
       61 agggcggcgg tgctccccca gataggagag gatgtcatgg gtgcgggaca gagggatgga
      121 cagatccccg tccttctttt tcatcttgtc cccgctgtac tgggccgggg tgtagtccgc
      181 cacggcggcg gccttaaaga taaagtcggc ctgctggcag gtgcgggaca ccgcctggaa
      241 catgtcctcc gcggagatta cagggaccac tgtgacgaag ggagggggct ccagggcggt
      301 gggaccagag atcagggtga cctccgctcc ccggagcatg gccatcctgg cgatggcgta
      361 acccattttt ccggtggagt ggttggtgag gtagcgcacc gggtccaggg actcctgggt
      421 gggaccggcg gtgaccagga cccgccggcc cgccaggtcg tgggggaggg ccaggacgcg
      481 gaggatgtgc tggaccagag tgtccggctc cggcatcttg ccgggcccca cagcgccgca
      541 ggccagccgg cccgaggcgg gggcgatgac ctcccaccca tagcggcgca gcagatccag
      601 gttgtcctgg gtcaccgggt tctcatacat cttggtgttc atggccggag cgatcagctt
      661 ggggcaggga caggccagca ctgtggtggt gagcatatcg tcggccagcc cgtgggccag
      721 cttggcgcac acattggcgg tggcgggggc gacgatcacc aggtcggtgc ggtcggccag
      781 ggcgatgtgc tccacctgat gggagaagtt gcggtcaaag gtgtccacca tggtccggtt
      841 cccggtgagc tgttcaaaag tgaggggggt gatgaactgg gtggcgttcc gggtcatcac
      901 cacctccact gaggcgtgga gcttcaccag cgcggaagcc agtgccgcgg ccttataggc
      961 ggcgatgccg ccggtgacgc ccaaaaggat ggtttttccg ctgagcattg caaatcgctc
     1021 ctttgcatag agcggcgcgg ggcgccgctg tcgttttttt cattatagct gaccaggcgg
     1081 cagattgtaa agggcagggg gatattttgc gcgagaggaa aagaattgca aagtttcggt
     1141 taattcctgc cttgtattcc caccggcggt cgggtataat gtggcagaat aaggacccgt
     1201 gggcgcgtct ccggcaaagg ggggctggcg ggtcagagaa aaaacggggg ccctccccgc
     1261 tgaggtgaac agatcatgct gatcgccatt gatatcggca ataccaatat cgttatcgga
     1321 ggcatccagg gcgacgccat ccagttcgag gccaggatcg ccaccgaccg catcaagacc
     1381 tccgaccagt acggggtgga gatcaagaac atcctggccc tgttcggggt gaagccctgc
     1441 gatgtggacg actgcatcat ctcctcggtg gtgccgccgg tcttcaactc ggtctacacc
     1501 ggactggtga agatgacggg aaagcacccc ctggtggtgg ggccgggcat caagaccggc
     1561 ctgaacatcc agatggacgt cccctcccag gtgggcagcg accgcatcgt catcgccgtg
     1621 gccgcgctgg cggagtatga gccccccctg atcctgatgg acctggggac ggccaccacc
     1681 atcgaggtgg tggaccgggg cagcacctac ctggggggct gcatcatccc gggggtgcgc
     1741 acctccctgg acgccctcac ctcccgggcg gcccagctgc ccggcatccg gctggaccgg
     1801 ccgggccggg tgatcggcaa gaacacggtg gactgcatgc gcagcggcat catgtacggc
     1861 accgccgcca tgctggacgg catgctggac cgggtggagg aggagctggg cagccgggcc
     1921 acggtggtgg ccacgggggg gatggcccag ttcatcgtgc ccctgtgccg ccgggagatc
     1981 aagctggaga aggacctgct gctcaagggc ctgaacatca tttaccagaa gaacgccgcc
     2041 aagcgggggg ggcgctgaga caaggaagaa aaaacttgca gctgatcata aaaatgggat
     2101 atgccaggaa ggcatatccc atttttaatg gaattatctg tatcaagatg ctgtcgtcac
     2161 ggagcgaagg aagtccaaaa gttcatcaaa tcgttcttga tccaccgcct catatttgct
     2221 ctcgcccaga taaatatatc cgttttcgaa aacggatata tttccgatct cttcaagctg
     2281 atcatttacc ttgaaaaacc agatctcata tatatgctgt ccggtttcaa atcgacacac
     2341 accgaaagag gggccgcagt atcgaatgtt cagtgcatcg atcatttcga tcagcggggt
     2401 gtcgtccagg ctgcgcgact ttgatgaaac tgcgtttggc atctccgtga cagtgtagtc
     2461 tgtggcctca gatggctgga aatccaggac attggacagt tctttgggag agaacacata
     2521 atagatccag ccaactaaaa tcaataccaa aaccagagca gccaatagac ccgccgcttt
     2581 atggcgctta ttgctttcaa ccgctgtcat atgggagtcc atcaacaacc gacttccttc
     2641 aatttatttt ggtcaatttc aataataaca tagattgtca atgcgtgtca atatgtcggt
     2701 ataagacaaa tttgttttgc ggctcccctg ctaggccatt tgtgagcaaa gagcaccgtg
     2761 atcagaagaa cgcttccggc cggaaggccg gcgttttttt ctataggcgg aaaaattttt
     2821 tcccctggcg gcgcgacctg ccggggcggg acatgctcta tataagtgaa gagacaacgg
     2881 aaaagaggtg aggccatgac ggatcaggaa ttggtagagc tgttctggca gcggcgggag
     2941 gaggccctga ccgggctcca ggacaagtac gggggctggt gccgctccat cgccggccgc
     3001 atcctggggg aagagcggga cgtggaggag tgtctggggg actgctggct ccagctgtgg
     3061 aacgccatcc cgcccgcccg gccccagcac ttccggggct atctgggggc ggtggtgcgc
     3121 aaccgggccc tgctcctccg ccggcggcgg ggacggcagg gggacacggt ctctctggcc
     3181 gctctggagc tggctgagtg cctccccagc cgggaggggc ccgaggaaca ggccgaggcg
     3241 gcggagctgg ggcgggccat ctccgccttc ctgagagagc tgggggaggg gccgcgtacc
     3301 gccttcctgc ggcggtactg gtacgcagac accgtggagg agaccgccgg gcacctgggc
     3361 tggtcgctgt ccaggaccaa gagcgtcctg tttcggacgc ggaagaagct gaaagcttat
     3421 ttggaacagg agggaatgtt gtgaaccgag gagaacgcat gatggaggct gtgggccttg
     3481 tggacccggc cttggtagaa caggccgccg gtccggcccg gcggcacagc gcccgccggc
     3541 ccctgcggac cggcctgatc gccgcctgct tgtgtgcggc cctgctgggg tcggcgtggg
     3601 cggccaacca gatcggaggg ttccggtccc tgaaattttt tga
//
LOCUS       DBMDSJ010000145         3641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6491_length_3641_cov_1.975424, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000145 DBMDSJ010000000
VERSION     DBMDSJ010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_3641_cov_1.975424"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="ACZPAQ_04595"
     CDS             complement(<1..453)
                     /locus_tag="ACZPAQ_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0903296.1"
                     /translation="MELNFDALVVGAGYAGAVCARELAERGGKRVLVLERRDHIGGNA
                     YDRLDEAGVLIHQYGPHIFHTNDEAVFRYLCRFTRWRDYQHRVVADLPAPDGGRMQMP
                     VPFNLTSLRKAFGPEAGKRLGEKLIAAYGAEKKVTILELRQNQDPELAK"
     gene            682..1050
                     /locus_tag="ACZPAQ_04600"
     CDS             682..1050
                     /locus_tag="ACZPAQ_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903297.1"
                     /translation="MKRFAAGLALLFLAITGCSAQEAASSTPHIYPPLIYVNDCLYHD
                     LGRFRAVTKLPDGWILWGEVEQRVPQTEEIPEENFTSNSLDPGTELFANETDDTKIYA
                     KLEEKYYLLYEQVIEEDADS"
     gene            complement(1169..1771)
                     /locus_tag="ACZPAQ_04605"
     CDS             complement(1169..1771)
                     /locus_tag="ACZPAQ_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR0903298.1"
                     /translation="MPNKAETFARSETHINLMRAFAGESQARNRYTIAAGMAREKNLY
                     VVQQVFLFTADQELTHAKQFYRQLADLSGQTVRADGTYPVDLYPDLLSHLRAAQHNEF
                     QEWEHDYAHFAKTASEEGFPLVAKLFDSIAGIERIHGERFGRFADLLEREELFVSSVT
                     CQWMCLNCGYVVESTMAPASCPVCHHPQGYFVRMEMVPFH"
     gene            2053..3501
                     /locus_tag="ACZPAQ_04610"
     CDS             2053..3501
                     /locus_tag="ACZPAQ_04610"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR0903299.1"
                     /translation="MKEKTNLTMLCDFYELTMGNGYFQTGLGERICYFDVFFRSVPDQ
                     GGFAIAAGLAQVIEYIQKLRFDEEDIAYLRSKGCFAEPFLDYLRRFRFTGDIWAVPEG
                     TPIFPGEPILTVRAPAIQAQFIETFVLLCLNHQSLIATKSNRIVRAAEGRPVAEFGSR
                     RAQGAEGAILGARASYIAGCSATACTMADQHYGSPATGTMAHSWVQMFPDEYTAFKTY
                     CQLYPNNATLLVDTYNVLRSGVPNAIRAFKEVLEPLGVTKCGIRLDSGDLTYLSRKAR
                     EMLDEAGLTGCKIVASNALDEYIIRDLIRQGARIDSFGVGERLITSRSEPVFGGVYKL
                     SAIEDDQGHILPKIKISENPAKITTPHFKKVYRIFSRDTGKAEADLICVHDEEIDFTH
                     PLELFDPAATWKRKVYTNIEARELLVPIFRGGELVYQVPELQTSRAYCQRQVDALWDE
                     VKRFENPHNYYVDLSQKLWDIKQSLLTSHEMA"
BASE COUNT          729 a         1149 c         1054 g          709 t
ORIGIN      
        1 cttggccagc tccgggtcct gattctggcg cagctccagg atggtgacct tcttctctgc
       61 gccgtaggcg gcgatcagct tctcccccag acgcttcccc gcctcgggtc cgaaagcctt
      121 ccgcagggag gtcaggttga agggcaccgg catctgcatc cggccgccgt ccggggccgg
      181 caggtcggcc accacccggt gctggtagtc ccgccaccgg gtgaagcggc acagatagcg
      241 gaataccgcc tcgtcattgg tgtggaaaat atgggggccg tactggtgga tcagcacgcc
      301 cgcctcatcc agccggtcat aggcgttgcc gccgatgtgg tcccggcgct ccagcaccag
      361 gacccgcttt ccgccccgct cagccagctc ccgggcacac accgcgccgg catagcccgc
      421 acccaccacc agggcgtcaa aattcaattc caaggggtct cccacctttt tctgtttttt
      481 ggggacaacg ccccatgccg ggacagtata tcataaattt ccctgaaagg aaataaagaa
      541 tcgattaaat gtggattgaa atggtgccgg acaacgggcg cagatataaa ttttacgatg
      601 taaaaatcct tatttacaaa tatcatatag attggtaata tatgggcaga tccaatttat
      661 aattggggat gatttgaaaa aatgaagcga tttgccgccg gcctcgcact tttattcctt
      721 gccatcacag gctgttctgc ccaggaagcg gcaagcagta cgccgcatat atatccccct
      781 ctcatttatg tgaatgattg tctttatcat gacctaggac ggttcagagc cgtcacaaaa
      841 ctgccagacg gatggatatt atggggagag gtggagcagc gtgtgcccca aaccgaggaa
      901 attccagaag aaaatttcac atccaactct ttagacccgg gaactgagct gtttgccaat
      961 gaaacggatg acacgaagat ctatgcaaaa ctggaggaaa agtattatct tttgtatgaa
     1021 caggttatag aagaggatgc ggactcctaa tgccgcagcg agtcaatcac tttaacagat
     1081 cgctgcaaca tatattgcag cgatcgtcct aaaagaggcg cggccctcct gctctccagg
     1141 agggccgcgc ctgtcttagt ctgggggatc agtggaaggg caccatctcc atgcgcacga
     1201 aatagccctg agggtggtgg cacacggggc agctggcagg ggccatggtg gactccacca
     1261 catagccgca gttcagacac atccactgac aggtcacgga ggagacgaac agctcctccc
     1321 gctccagcag atcggcgaag cggccgaacc gctcgccgtg gatgcgctcg atgcctgcaa
     1381 tggagtcgaa cagcttggcc accaggggga agccctcctc actggctgtc ttggcgaagt
     1441 gggcgtagtc gtgctcccac tcctggaact cattgtgctg ggccgcccgc aggtgggaga
     1501 gcaggtcggg atacaggtcc accggatagg taccgtccgc ccggacggtc tggccggaca
     1561 ggtccgccag ctgccggtaa aactgcttgg cgtgggtcag ctcctggtct gcggtgaaca
     1621 gaaagacctg ctggaccacg tacaggtttt tctctctggc cataccggcg gcgatggtat
     1681 agcggttccg ggcctggctc tcccccgcga aggcccgcat caggttgatg tgggtctcac
     1741 tgcgggcgaa ggtctctgct ttgtttggca tgagattacc tccttttctg aactggttcc
     1801 cgttcagtat gcccgcgggg gaaactcctt attccagtat caggaagagc ctccctgtcc
     1861 gacatggcat ctgccgcacc acctttctgg ggatgtttgg cgagatatcc ccccgctgag
     1921 gccgggcccc gcgccctttt cccttgcctt ttccctcttt tccaggtata ataatgaaaa
     1981 tacacacggg tgttgacacc cgcgggggcg ggagcggagc atccccaccc cgaagaggag
     2041 gcatccccag ggatgaaaga aaagacaaat ctgaccatgc tgtgcgactt ctatgagctc
     2101 accatgggca acgggtactt ccagaccgga ctgggcgagc gcatctgcta ctttgacgtg
     2161 ttcttccgct cggtcccgga ccagggaggc tttgctatag cggcggggct ggcccaggtc
     2221 atcgagtata tccagaagct gcgctttgac gaggaggaca tcgcctacct ccgctccaag
     2281 ggctgctttg ccgagccctt cctggactat ctgcgccggt tccgtttcac cggcgacatc
     2341 tgggccgtgc cggagggcac ccccatcttc cccggggagc ccatcctcac cgtgcgggcc
     2401 ccggccatcc aggcccagtt catcgagacc ttcgtgctac tgtgcctgaa ccaccagagc
     2461 ctcatcgcta ccaagtccaa ccgcatcgtc cgggcggcgg agggccgtcc ggtggccgag
     2521 ttcggctccc gccgggccca gggggcggag ggagccatcc tgggcgcccg ggcctcctac
     2581 atcgctggct gctccgccac cgcctgcacc atggccgatc agcactacgg ctcccccgcc
     2641 accggcacca tggcccactc ctgggtgcag atgttccccg acgagtacac cgccttcaag
     2701 acctactgcc agctctaccc caacaatgcc accctgctgg tggacaccta caatgtactg
     2761 cgctccggcg tgcccaacgc catccgtgcc ttcaaggagg tgctggagcc cctgggcgtc
     2821 accaagtgcg gcatccggct ggactccggc gacctgacct atctgtcccg gaaagcacgg
     2881 gagatgctgg acgaggccgg cctcaccggc tgcaagatcg tggcctccaa cgccctggac
     2941 gagtacatca tccgggatct gatccgccag ggggcccgca tcgattcctt cggggtgggc
     3001 gagcggctca tcacctcccg ctccgagccc gtcttcggcg gcgtgtataa gctgtccgcc
     3061 atcgaggatg accaggggca catcctcccc aagatcaaga tcagcgaaaa ccccgccaag
     3121 atcaccaccc cccatttcaa gaaggtgtac cgcatcttct cccgggacac cggcaaggcg
     3181 gaggccgacc tgatctgcgt ccacgacgag gagatcgact tcacccatcc cctggagctc
     3241 tttgacccgg ccgccacctg gaagcggaag gtgtacacca acatcgaggc ccgggagctg
     3301 ctggtcccta tcttccgggg aggcgagctg gtctatcagg tgccggagct ccagaccagc
     3361 cgggcctact gccagcggca ggtggacgcc ctgtgggatg aggtcaagcg ctttgaaaac
     3421 cctcacaatt actacgtgga cctgtcccag aagctgtggg acatcaagca gtccctgctc
     3481 accagccacg agatggcata agaggacccc cggcggcggg agcttcccgc cgccgggggt
     3541 ttttttgcgc cttttgggga atgaggagcc ccctgtcacc tgccggtcac agacagctcc
     3601 aggcccggat aggcggccgc cgggtccgtg gggacgccgt t
//
LOCUS       DBMDSJ010000146         3630 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6509_length_3630_cov_1.494191, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000146 DBMDSJ010000000
VERSION     DBMDSJ010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6509_length_3630_cov_1.494191"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="ACZPAQ_04615"
     CDS             <1..480
                     /locus_tag="ACZPAQ_04615"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR0903300.1"
                     /translation="VAAVAVHGRTRAQMYAGRADWDHIRAVKEAVKIPVIANGDVFKP
                     EDAPRILAYTGADMAMIGRGVFGNPWLFAQCRAALEGREIPPMPPLAERCDTAVRQFQ
                     LSAANKGEKIACLEARRHYAWYLKGVPHAGYYKEQIVKITTLEDVYRVTKGIKRDLC"
     gene            527..1141
                     /locus_tag="ACZPAQ_04620"
     CDS             527..1141
                     /locus_tag="ACZPAQ_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903301.1"
                     /translation="MTDQELAARARDGDQDAFEQLVRDNEKRIYTLALRMTGSREDAL
                     DLAQDAFFQAWKALPTFQGESSFATWLYRLATNLCLDHLRTQKRRTQSMGPALSLDDE
                     ENGPVQVADQQLQPQEAVERSERRRALERGLASLPDHHRQVLIMRELSGLSYQEIAQV
                     LDLDLGTVKSRIARARLSLRKILLEDGNFFDSPASMEIERHRKE"
     gene            1148..1978
                     /locus_tag="ACZPAQ_04625"
     CDS             1148..1978
                     /locus_tag="ACZPAQ_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024881.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR0903302.1"
                     /translation="MSGCERALDLIGLRLDGPLSPEEQRELDAHLEQCPACRAAAREL
                     EELEGALVQLGECDPPPQLVSGVMERVRADSAPAKVVPLWKRPAFRGALGMAACAALC
                     IGLLPQMAGLSSGGGNSTGAAPASASESQAASSTPAAGAYAAGGESSPSAEVRSSNAG
                     RPEAEDTAPMEAESPASIAPFSLQEQGAGAPEDFSALAPALSEALAGQLGQSPGRLLL
                     FSTIPEELEDRDWQETDGFRWTALEAEGDHSLLDALTAQAAALWESGVEGPAVALVYP
                     "
     gene            2215..>3630
                     /locus_tag="ACZPAQ_04630"
     CDS             2215..>3630
                     /locus_tag="ACZPAQ_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR0903303.1"
                     /translation="MSYSVQNIRNVCLLGHGGSGKTSLAESLLYMTGAIDRMGKPADG
                     NTVCDYDAEETRRQISISTSVAPLEYKGCKINILDTPGSFDFSGEVLEALQVSDAAII
                     VCSAKDGISVGLEKAWRYCEERDMPRFIYISKTDEENSDYNATFDALRERFGNKIAPI
                     VVPIWDDNKKVTGIIDVLNKRAYEMQNGKRVEIELPEGKEDVVTQFNDALKESVAETS
                     EEFMDKFFSGEDFTYVEMIQGLRQGVRELSLFPVLCGSAVSTMGSLMLMDNIVELLPN
                     PVEGNMRKGMLPSGDKEEYAVSPGGVPSAFVFKTVSDQYGKYSFIKVISGTITSDLAL
                     VNARTGSPEKLGRLYTMRGKKAVEVKELSCGDIGAIGKLEKVKTGDTLCDARKVFFMP
                     PIPFPEPCYSVAIVPKTRGQEDKIAQGLARLNEEDPTFTLVNNGETHQMVLSGTGDMQ
                     MDVLVSKLKSRFNVEAELKAPR"
BASE COUNT          662 a         1185 c         1156 g          627 t
ORIGIN      
        1 gtggccgccg tggccgtcca cgggcggacc cgggcccaga tgtacgccgg ccgggcggac
       61 tgggaccaca tccgcgccgt gaaggaggcg gtgaagatcc ctgtgatcgc caacggcgac
      121 gtgttcaagc cggaggacgc cccccgtatc ctggcctaca ctggggcgga catggccatg
      181 atcggccggg gggtattcgg caacccctgg ctgttcgccc agtgccgggc agctctggag
      241 ggccgcgaga tccctcccat gccgccgctg gcggagcgtt gcgacacggc ggtgcgccag
      301 ttccagctct ccgccgccaa caagggggag aagatcgcct gtctggaggc ccggcgccac
      361 tacgcatggt atctgaaggg ggtcccccac gcgggatatt ataaggagca gatcgtgaag
      421 atcactaccc tggaggacgt gtaccgggtg acaaagggga tcaagcggga cctgtgctga
      481 ccccggccgc ggggccggag ggtttggatt tggaaggggt gaaggtatga ctgaccagga
      541 gctggcggcc cgcgcccggg acggggacca ggacgccttc gaacagctgg tccgggacaa
      601 cgaaaaacgg atctataccc ttgcgctgcg gatgacgggc agccgtgagg atgctctgga
      661 tctggcccag gatgcctttt tccaggcgtg gaaagccctc cccacctttc agggggagag
      721 cagctttgcc acctggctct accgcctggc caccaacctg tgcctggacc atctgcggac
      781 tcagaagagg cggacccagt ccatggggcc cgccctctcc ctggacgacg aggaaaacgg
      841 tcccgtccag gtggcggacc agcagctcca gccccaggag gcggtggagc gctcagagcg
      901 ccgccgggcc ctggagcggg ggctggcctc cctcccggat caccaccgcc aggtgctgat
      961 catgcgggag ttgtccggac tctcctacca ggagatcgcc caggtcctgg acctggatct
     1021 ggggactgtg aaatcccgta tcgcccgggc tcgtctctcc ctgcgaaaaa ttttgctgga
     1081 ggatgggaac ttttttgact ctccggcgtc tatggagata gaacgacaca gaaaggagtg
     1141 acccggcatg tcaggctgtg aacgtgcgct ggacctgatc ggcctgcgtc ttgacggccc
     1201 cctgtccccg gaagagcagc gggagctgga cgcccacctg gaacaatgcc ctgcctgccg
     1261 ggccgccgcc cgggagctgg aggagctgga gggggccctc gtccagctgg gtgagtgcga
     1321 tcccccgccc cagctcgtct ccggcgtgat ggagcgggta cgcgccgaca gcgcccctgc
     1381 caaggtcgtc ccgctctgga aacgccctgc cttccgggga gcactgggca tggcggcctg
     1441 cgccgccctg tgcatcggcc tgcttcccca aatggcgggt ctctcctccg gcggcgggaa
     1501 cagcaccggc gccgcccctg ccagcgcctc ggagtcccag gccgcttcct ccactcccgc
     1561 tgccggtgcc tatgctgccg gcggggagtc ctccccctct gccgaggtcc gctccagcaa
     1621 tgcgggccgt ccggaagccg aggacaccgc cccgatggag gcggagtccc ccgcctccat
     1681 cgctcccttc tccctgcagg aacagggagc gggcgcaccg gaggacttct ccgcccttgc
     1741 ccccgccctg tccgaagccc tggccggaca gctgggccag agtcccggcc ggctgctcct
     1801 gttctccacc attccagagg aactggagga ccgggactgg caggagacgg acggctttcg
     1861 ctggaccgcc ctggaggcgg agggggacca ctccctgctg gacgccctga ccgcccaggc
     1921 cgccgccctg tgggagagcg gggtggaggg ccccgcagtg gctctggtgt atccctgaat
     1981 cttacttcag ccctggaccg ggtgcgattt gtgcccggtc cagggctctt ttctgctttt
     2041 tttcgtggga gagccgcttt ttcttcattt ttgtttcatt ttacgtctgc gacaaaaaag
     2101 aggttgtcag atgtgcattt ttacgatata atagccaata gcagatcacc tcgccacggc
     2161 ggcttccgcc tggggagtct gtatgagtca gtccaaacat aaggagtggt aacaatgagc
     2221 tattctgtac aaaacatccg caatgtctgt ctgctgggac acggtgggag cggaaagacc
     2281 tccctggcgg agagcctgct ttacatgacc ggcgctatcg accgcatggg caagcccgcc
     2341 gacggcaaca ccgtctgcga ctatgatgcg gaggagaccc gccgccagat ctccatctcc
     2401 acttccgtgg ctcccctgga atataagggc tgcaagatca acattctgga caccccgggc
     2461 agcttcgact tctcaggtga ggtgctggag gccctccagg tctctgacgc cgccatcatc
     2521 gtctgctccg ccaaagacgg catctctgtg gggctggaaa aagcctggcg ctactgcgag
     2581 gagcgcgaca tgccccgctt tatctatatt tccaagacag atgaggaaaa ctccgactat
     2641 aacgccacct tcgacgccct gcgtgagcgc ttcggcaaca agatcgcccc catcgtggtc
     2701 cccatctggg acgacaacaa gaaggtcacc ggcatcatcg acgtgctcaa caagcgcgcc
     2761 tacgagatgc agaacggcaa acgggtggag atcgagctgc ccgagggcaa agaggacgtg
     2821 gtcacccagt tcaacgacgc tctgaaggaa tccgtggccg agaccagcga ggagtttatg
     2881 gacaaattct tctccggcga ggacttcacc tatgtggaga tgatccaggg cctgcgccag
     2941 ggcgtgcggg agctgtccct cttccccgtg ctgtgcggct ccgccgtgtc caccatgggc
     3001 tccctgatgc tgatggacaa tatcgtggag cttctgccca acccggtgga gggcaacatg
     3061 cgcaagggca tgctccccag cggcgacaag gaggaatacg cagtctcccc cggcggcgta
     3121 cccagcgcct tcgtgttcaa gacggtctcc gaccagtacg gcaagtactc cttcatcaag
     3181 gtcatctccg gcaccatcac ctccgacctg gccctggtca acgcccgcac cggctccccc
     3241 gagaagctgg gccgactgta caccatgcgg ggcaagaagg ccgtggaggt caaggagctc
     3301 tcctgcggcg atatcggcgc catcggcaag ctggagaagg tcaagaccgg tgataccctg
     3361 tgtgacgccc gcaaggtctt cttcatgccc cccatcccct tccccgagcc ctgctactcc
     3421 gtggccatcg tgcccaagac ccgggggcag gaggacaaga tcgcccaggg cttggcccgc
     3481 ctgaacgagg aggacccgac cttcaccttg gtgaacaacg gcgagaccca ccagatggtc
     3541 ctcagcggca ccggcgacat gcagatggat gtgctggtgt ccaagctgaa gagccgcttc
     3601 aatgtggagg cggagctgaa ggctccccgg
//
LOCUS       DBMDSJ010000147         3598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6561_length_3598_cov_1.935945, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000147 DBMDSJ010000000
VERSION     DBMDSJ010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6561_length_3598_cov_1.935945"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="ACZPAQ_04635"
     CDS             <1..803
                     /locus_tag="ACZPAQ_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0903304.1"
                     /translation="PGRIYGLIGRNGAGKTTLLGILTGQNTKDSGQVTCDGEPVWENC
                     RALEQICFSRELQPTLFSGPNNQKVRYYLKSAAIFYPNWDQEYADRLLNEFQLEPKKK
                     ISQLSKGQMSMVTILIALASRAPITILDEPAAGLDVVMRERFYQLLLEDFAQTGRTFL
                     VSTHIIEEAASVFEQVIILDQGRILENSPTEELVEQFRYISGREDVVDQACAGLEVLS
                     VQQMGRHKTAAVRGSDAQFRQARQADVDVSPMNLQNVFVALCGHGDAQ"
     gene            816..1478
                     /locus_tag="ACZPAQ_04640"
     CDS             816..1478
                     /locus_tag="ACZPAQ_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903305.1"
                     /translation="MRKEFRFLLQSARINLALVAGYAAVLTLGAILFPAPEGERNLFY
                     NYFISAPITVSFILFIMSFSMGANNLNISLTLGGRRRDYFWALQPVLALYVGTSWAIQ
                     LLMSAVPKALRWTDPWGWDLSLLGGWPLWVYPLVCWTVLAFGTLCGLLMVESRVWRGI
                     VVALAAMVMTAALIFLMVMAAGPKIPLWGDLPYILGGVLAVVLAVSEFFLWRRITLFS
                     VR"
     gene            1483..2208
                     /locus_tag="ACZPAQ_04645"
     CDS             1483..2208
                     /locus_tag="ACZPAQ_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903306.1"
                     /translation="MMMKMIRANWVDLAQGCGVVAGIFLAIHLITAGVLFWGHPDSSV
                     TISGIALPAAAGLMILVVTATYLAITFDQMVRFSLTRRRALGLLMGMAGAQALCSMGL
                     AALLTWVERATAPMLWKAISGAASVTLERSGQVIPELGRTAAAEAVPSLLVVDMYLEW
                     WWYPAIVLAAIALGLIIGGVIQRFGGKGGWFLWAIWMAVIFGDSLFGGFVFLKDLVPL
                     MVVLLAGSLVWSVWSLLHAVVKN"
     gene            2224..2430
                     /locus_tag="ACZPAQ_04650"
     CDS             2224..2430
                     /locus_tag="ACZPAQ_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903307.1"
                     /translation="MIPAGAIRKRAIIRQFLEAGAVSEETARTPEEAGVFQGLGIKFD
                     QLVERGVLVPCAGGRYYVDQCKSG"
     gene            complement(2566..>3598)
                     /gene="thrS"
                     /locus_tag="ACZPAQ_04655"
     CDS             complement(2566..>3598)
                     /gene="thrS"
                     /locus_tag="ACZPAQ_04655"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR0903308.1"
                     /translation="TPIMLNRQLWERSGHWDHYKNNMYTTVIDDVDFAVKPMNCPGGM
                     LVYASQPHSYKELPLRVGEIGLVHRHELSGALHGLFRVRCFNQDDAHVFMTRDQMQDV
                     IQETVRLFDEVYSVFGLSYTIELSTMPEDHIGTVEEWERNQDILKNAITAMGKDFVIN
                     EGDGAFYGPKLDFHLADSLGRTWQCGTIQLDSQLPERFELEYTGEDGQKHRPVMIHRV
                     VLGSIERFIGVITEHFAGAFPAWLAPVQVKVLPVTDRAAQYADQISKDLDGAGFRVEV
                     DHRNEKIGKKIREATMEKVPYMLVVGDRDMEGQTVSVRMRSGEDLGAMSCVQFQALLA
                     EDVRTKAIK"
BASE COUNT          607 a         1030 c         1229 g          732 t
ORIGIN      
        1 agccgggcag gatctatggg ctgatcggac gaaacggggc gggtaagacc accctgctgg
       61 gcatcctcac aggccagaac accaaggaca gcgggcaggt gacctgcgat ggagagccgg
      121 tgtgggagaa ctgcagggcg ctggagcaga tctgcttttc ccgggagctc cagcccaccc
      181 tgttctcagg tcccaacaac cagaaggtcc ggtactacct gaagtcggcc gccatattct
      241 accccaactg ggaccaggag tatgccgacc gcctgctgaa cgagtttcag ctggaaccaa
      301 aaaagaagat cagtcagctg tccaaggggc agatgtctat ggtgaccatc ctgatcgccc
      361 tggccagccg ggcacccatc accattctgg atgagccggc ggcggggctg gatgtggtga
      421 tgagggagcg gttctatcag ctgctgctgg aggactttgc ccagacgggg cggaccttcc
      481 tggtctctac tcacatcata gaggaggccg cctccgtctt tgagcaggtc atcatcctgg
      541 accaggggcg catcctggaa aacagcccca cagaggaact ggtggagcag ttccgctata
      601 tctccggccg ggaggacgtg gtggaccagg cctgtgccgg cctggaggtc ctctccgtcc
      661 agcagatggg gcggcacaag accgctgccg tccgggggag cgacgcccag ttccgtcagg
      721 cccggcaggc cgatgtggat gtgtccccca tgaaccttca gaacgtgttt gtggccctgt
      781 gcggccacgg tgatgcccag taaggagggg aaggtatgag aaaggaattc cgcttcctgc
      841 ttcagtcggc caggatcaat ctggccctgg tggcgggata tgcggcggtg ctcaccttgg
      901 gagccatcct gttcccggcc ccggagggcg agcgaaatct gttctacaat tatttcattt
      961 ccgcgcccat caccgtctcg ttcatcctgt ttatcatgag cttttccatg ggcgcaaaca
     1021 acctaaacat ctccctcact ctgggcgggc ggcggcggga ctacttctgg gcccttcagc
     1081 cggtcctggc gctctatgta gggaccagct gggccatcca gctgctgatg tcggcggtgc
     1141 ccaaggcgct ccggtggacc gatccctggg ggtgggatct ctcactgctg ggaggctggc
     1201 ccctgtgggt gtaccccctg gtgtgctgga cagtcctggc ctttgggacc ctctgcggtc
     1261 tgctgatggt agagtccagg gtatggaggg ggatcgtggt tgctttggcc gcaatggtaa
     1321 tgacggcagc cctgattttc ctgatggtga tggcggcggg gcccaagatc cctctgtggg
     1381 gcgatcttcc ctatatcctg gggggcgttc tggcagtggt cctggcggtc agcgagttct
     1441 tcctgtggcg taggatcacc cttttttctg tgaggtgagc atatgatgat gaaaatgatc
     1501 cgggccaact gggtggatct ggcccagggc tgcggagtgg tggcggggat tttcctggcc
     1561 atccacctga tcacggcggg ggtgctcttc tggggccatc cagacagcag cgtcaccatc
     1621 tccgggatcg ctctgcccgc ggcggcgggg ctgatgatcc tggtcgttac cgccacctat
     1681 ctggccatca cctttgacca aatggtgcgc ttcagcctga cacgccgccg ggcactgggg
     1741 ctgctgatgg ggatggccgg ggcacaagcg ctgtgctcca tggggctggc cgccctgttg
     1801 acctgggtgg agcgggccac ggcgcccatg ctgtggaagg ccatttccgg cgcggcgtcg
     1861 gtgacgctgg agcggagcgg acaggtgatc ccggagctgg ggcgcacggc ggcagcggag
     1921 gcggtgccct ccctgctggt ggtggatatg tatttggaat ggtggtggta tccagccatc
     1981 gtcctggccg ccattgcgct ggggctgatt atcggaggcg tgatccagcg ctttggagga
     2041 aaaggaggct ggttcctgtg ggccatctgg atggcggtca tattcggtga ttccctcttc
     2101 ggcggctttg tgttcctgaa ggatctggtc cccctgatgg tggtcctgct ggcggggtcc
     2161 ttggtctggt ccgtgtggtc cctgctccac gcagtggtga aaaactgaaa ggagggggag
     2221 gccatgatac cggcgggtgc catccgaaag agagccatca tcaggcagtt cctggaggcg
     2281 ggggcggtct cggaggagac ggccagaacg ccggaggagg cgggggtctt ccaggggctg
     2341 ggcatcaaat ttgaccagct ggtggagcgg ggcgttctgg tcccctgtgc tggaggacgg
     2401 tattacgtgg accagtgcaa aagcggttga aaaagggagg cgcttccgcc ggaagcgcct
     2461 cccttttttc attatgccgg tattccccgg cgccgcagtc atttcccgcg ccgggaccag
     2521 acttctatgg tatccaaggg atggatcgaa tgctctgaaa gccgcttatt tgatggcctt
     2581 ggtccgcacg tcctcggcca gaagggcctg gaactggacg caggacatgg cgcccaggtc
     2641 ctcaccggag cgcatgcgga cagagacggt ctgaccctcc atgtcccggt cgcccaccac
     2701 cagcatatag gggaccttct ccatggtggc ctcccggatc ttcttgccga tcttctcgtt
     2761 gcggtggtcc acctctaccc ggaaaccggc cccatccagg tccttggaga tctggtcggc
     2821 gtactgggcg gcccggtcgg tgacaggcag gaccttcacc tggacggggg cgagccatgc
     2881 ggggaaagcg ccggcaaagt gttcggtgat gacgccgatg aaccgctcga tggagcccag
     2941 caccacccgg tggatcatca cggggcggtg cttctggccg tcctcgccgg tgtactccag
     3001 ctcgaagcgc tcaggcagct ggctgtccag ctggatggtg ccgcactgcc aggtgcggcc
     3061 cagggagtcg gccagatgga agtccagctt ggggccgtag aaggcgccgt ccccctcgtt
     3121 gatgacgaaa tccttgccca tggcggtgat ggcgtttttc aggatgtcct ggttgcgctc
     3181 ccactcctcc acggtgccga tgtggtcctc tggcatggtg gacagctcaa tggtgtagct
     3241 caggccgaag acggagtaga cctcgtcgaa caggcggacg gtctcctgga tcacgtcctg
     3301 catctggtcc cgggtcataa agacatgggc gtcgtcctgg ttgaagcagc gcacccggaa
     3361 caggccgtgg agggcgccgg acagctcgtg gcggtggacc aggccgatct cgcccacccg
     3421 caggggcagc tccttatagg agtggggctg gctggcatag accagcatcc cgccggggca
     3481 gttcatgggc ttgacggcga agtccacatc gtcaatgacg gtggtgtaca tattgttctt
     3541 atagtggtcc cagtggccgg agcgctccca cagctgacgg ttgagcatga tgggggtg
//
LOCUS       DBMDSJ010000148         3596 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6567_length_3596_cov_1.284979, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000148 DBMDSJ010000000
VERSION     DBMDSJ010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_3596_cov_1.284979"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="ACZPAQ_04660"
     CDS             <1..1237
                     /locus_tag="ACZPAQ_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR0903309.1"
                     /translation="ENDNDVAAVFDRAACRVCRTCSLRSSCWERDYVATFNALNDATA
                     PMLERGRGVASDYPRYFADRCLHFPAFVEAVNQELTALLYRRQYNSRIRESRAAVCRQ
                     YGELSALLGTAAAELSRELIPDPAGNQKLRRHLALLGLEMETAVYRDEYGRLRAELKG
                     EGCQTLDTPEELAALSDLLGVPTRAEAGEESLTLIQLEPLMAVAGVAARKKDGETVSG
                     DAGTYFKREDGTLYVLLCDGMGSGPGAGRESSLAVRLLEQFLMAGVETGHALATLNSA
                     LALRGEEEGGFTTVDLLQVDLFTGEGAVYKLGAAPTYVRRGSSVRRIAGSSLPAGLAP
                     SEHAVPDHTRLRLEAGDCVLMVSDGIAGTGDDAWVRERLAKFDGESPKELARSLITDS
                     PEGATDDRTALVVRIARRT"
     gene            1565..1840
                     /locus_tag="ACZPAQ_04665"
     CDS             1565..1840
                     /locus_tag="ACZPAQ_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 2"
                     /protein_id="HIR0903310.1"
                     /translation="MVKGITRQVILVKSPDPKLFEEAIFIVKEEALAREGVSADQVIR
                     EARQAADGYLRRSRAWNARGRQLPGPAWGAAGAVLASLAWGLGLFLL"
     gene            1938..>3596
                     /gene="asnB"
                     /locus_tag="ACZPAQ_04670"
     CDS             1938..>3596
                     /gene="asnB"
                     /locus_tag="ACZPAQ_04670"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR0903311.1"
                     /translation="MCGICGFCDFTRDNAGPVWREAGERMGETLAHRGPDGKGLWQGA
                     DCVLVHRRLAVIDPERGQQPMARVRDGVEYVLIYNGELYNTDELRRELRKLGFSFETE
                     SDTEVLLNACIAWGDGAAQRLEGIFAFAFWDGRRRRLMCCRDRFGVKPFFYAWKDGAL
                     VFASELKALFQYPGLEPRLGRQGLCEVLGIGPARTAGAGVFEGVRELKGGCQLTAGPE
                     GLQIRRYWALESRPHEDGYEETVEKVRHLLEGAIRRQLVSDVPLCTFLSGGLDSSIIT
                     AVAARAYQEQGREPLETYSFDYVGNRKHFKASAFQPDADAPWVKKAVRALGTRHRVLR
                     CDIPSLLQLLPEAVEAKDLPGMADVDSSLLYFCRKVAERHTVALSGECADEVFGGYPW
                     FERSELLEADTFPWCPDLEFRRAVVKPELWESLQVEDYVQARYQQSLAEMPALPGEAL
                     WERRRREVGWLSLNWFMSTLLDRKDRMSMAAGLEVRVPFCDHHLVEYVWNIPWAMKAM
                     NGQRKQVLRDAAEGLLPEELLRRPKSPYPKTHDPLYEQLVRARLL"
BASE COUNT          598 a         1067 c         1350 g          581 t
ORIGIN      
        1 ggagaacgac aacgatgtgg cggcggtctt tgaccgggcg gcctgtcggg tgtgccggac
       61 ctgctccctc cggtccagct gctgggagcg ggactatgtg gccaccttca acgccctcaa
      121 cgacgccacg gcccccatgc tggagcgggg gcggggagtg gccagcgact acccccgcta
      181 cttcgccgac cgctgcctcc acttccccgc ctttgtggag gcggtgaacc aggagctcac
      241 cgccctgctc taccgccggc agtacaacag ccgcatccgg gagagccggg cggcggtgtg
      301 ccggcagtat ggggagctgt ccgcccttct ggggacggcg gcggcagagc tgagccggga
      361 gctgatcccg gacccggcag gcaatcagaa gctgaggcgg cacctggctc tgctgggact
      421 ggagatggag acggcagtgt accgggacga gtacggccgt ctgcgggcgg agctgaaggg
      481 ggagggatgc cagactctgg acacaccgga ggagttggcc gccctatcgg atctgctggg
      541 tgtgcctacc cgggcggagg cgggggagga gagcctgact ctgatccagc tggagccgct
      601 gatggcggta gcgggcgtgg cagcccggaa gaaggacggg gagacggtga gcggggacgc
      661 cgggacctac ttcaagcggg aggacggcac cctctatgtg ctgctgtgcg acggcatggg
      721 gagcgggccc ggggcgggcc gggagagctc cctggctgtc cgcctgctgg agcagttcct
      781 gatggcgggg gtggagacgg gccacgccct ggccacactg aattctgccc tggcccttcg
      841 gggagaggag gagggggggt tcaccacagt ggacctgctc caggtggacc tgttcacagg
      901 ggagggggcg gtgtacaagc tgggagctgc ccccacctat gtgcggagag ggagctcggt
      961 gcggcggatc gcaggcagct ccctgcccgc tgggctggcc ccttcggagc acgcggtgcc
     1021 cgaccatacc cggctgcggc tggaggcggg ggactgcgtc cttatggtca gcgacggcat
     1081 cgccggaact ggggacgacg cctgggtccg ggagcggctg gctaaattcg atggggagag
     1141 ccccaaggag ctggcccgga gtctgatcac cgacagcccg gagggagcca ccgatgaccg
     1201 caccgccttg gtggtgcgga tcgcgcggag aacgtagagg tcaagtgcgc ggaccctttg
     1261 cctggcagca aatgcccctg gcggccgtat ggatgcggcc gccaggggca ttttcctacg
     1321 ggatcaaagc cgcccctgga gcaccgggat gagaaggatc acggccagat tgaccgcaca
     1381 taccagcgcc agggacagca gctgctcctt ggattcatcg tccatctccg cccctccttc
     1441 agatctcttg gcaagatcca gtgtacggcg ggccgggccg gaatataccc ggtaaaattg
     1501 ggcaagaata actgtgtaca cagggggctc acggcagggg ccccggaaac ggaggaggta
     1561 tctcatggtc aaaggcatca cacgccaggt catcctggtg aaatccccgg accccaagct
     1621 cttcgaggag gccattttta tcgtgaaaga agaggcattg gccagagagg gggtcagcgc
     1681 ggaccaggtc atccgggagg ccagacaggc ggcggacggc tatctccgcc gcagccgggc
     1741 atggaacgcc cggggcaggc agcttcccgg accggcctgg ggagctgcgg gggcggtcct
     1801 ggcctcgctg gcctgggggc tgggtctgtt tctgctgtaa cagacctgag acgcatgcat
     1861 acaatgggat gtggaccggg cggcggcgga cccgtctgcc cgggctcccg gtccaaggct
     1921 gataaaggag cttacttatg tgcggtatct gcggattttg tgattttacc cgggacaacg
     1981 cgggcccggt ctggcgggaa gcgggagagc ggatggggga gacccttgcc caccgggggc
     2041 cggatggaaa gggactctgg cagggagcgg actgcgtcct ggtccaccga cgcctggcgg
     2101 tcatcgaccc ggagcggggg cagcagccta tggcccgggt gcgggacggc gtggaatatg
     2161 tgttgatcta caacggcgag ctctataaca ccgatgagct gcgccgggag cttcggaagc
     2221 tgggcttctc ctttgagacg gagtcggaca ccgaggtgct gctcaacgcc tgcatcgcct
     2281 ggggggatgg ggcggcccaa cggctggagg gcatcttcgc ctttgctttc tgggacggga
     2341 ggcggaggcg gctgatgtgc tgcagggacc ggttcggcgt caagccattc ttctatgcct
     2401 ggaaggacgg ggcactggtg tttgcatctg agctgaaggc actgttccaa tatccgggcc
     2461 tagagccacg gctgggccgg caggggctgt gcgaggtgct gggcatcggc cccgcccgta
     2521 cagcgggggc aggtgtattc gagggggtcc gggaactgaa ggggggctgt cagctgacgg
     2581 ccggcccgga gggcctccag atccgtcggt actgggcact ggagagccgc ccccacgagg
     2641 acggctacga ggagacggtg gagaaggtgc gccatctcct ggagggagcg atccggcggc
     2701 agctagtcag cgacgtgccc ctgtgcacct tcctgtctgg cgggctggac tcctccatca
     2761 ttaccgcggt ggccgcccgg gcctatcagg agcaggggcg ggagcccctg gagacctact
     2821 cctttgacta tgtgggcaac cggaagcatt tcaaggcgtc cgctttccag ccggacgccg
     2881 acgctccctg ggtaaagaag gcggtccggg cactgggtac ccgacaccgg gtgctgcggt
     2941 gcgacatccc ctccctgctc cagctgctcc ctgaggccgt ggaggccaag gacctgccgg
     3001 ggatggcgga tgtggactcc tccctgctgt atttctgccg gaaggtggcg gagcggcaca
     3061 cggtggccct ttccggggag tgcgctgacg aggtgtttgg cggctacccc tggtttgagc
     3121 ggtcggagct gctggaggcg gacaccttcc cctggtgtcc ggacctggag ttccgaaggg
     3181 cggtggtgaa gccagagctg tgggagagcc tccaggtgga ggactatgtc caggcccgct
     3241 accagcagtc cctggcggag atgccggccc tgccggggga ggccctgtgg gaacgccgcc
     3301 gccgggaggt cggctggctg tccctgaact ggttcatgtc caccctgctg gaccggaagg
     3361 accgcatgag tatggccgcc gggctggagg tgcgggtccc cttctgtgac caccatctgg
     3421 tggagtatgt gtggaacatc ccctgggcaa tgaaggctat gaacgggcag cgcaagcagg
     3481 tgctgaggga cgcggccgaa ggcctgctcc cggaggagct gctccgccgg cccaagagcc
     3541 cctaccccaa gacccacgac cccctgtatg agcagctggt ccgggccagg ctgctc
//
LOCUS       DBMDSJ010000149         3586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6585_length_3586_cov_1.632425, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000149 DBMDSJ010000000
VERSION     DBMDSJ010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6585_length_3586_cov_1.632425"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="ACZPAQ_04675"
     CDS             <1..728
                     /locus_tag="ACZPAQ_04675"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR0903312.1"
                     /translation="YGRETQGALDITAYSAVHAWGFTTGEYRVPGPEELAELAARIDY
                     TQVELDPEGCRVRLPQGMQLDLGSIAKGWASDLLAELAGGEPAIFNLGGNVRTAGDKP
                     DGSPWRIGIQTPEAGSSGYLGVLELTGSQAVITSGGYERYFEEDGQTYWHIMDPETAA
                     PARSGLKSVTIVSPLGVYCDALSTAVFVRGADWGADFWRTHQDFEMVLVLEDGSLWVT
                     AGLAGSFTLAEDYQDLEVTVIQL"
     gene            725..1099
                     /locus_tag="ACZPAQ_04680"
     CDS             725..1099
                     /locus_tag="ACZPAQ_04680"
                     /inference="COORDINATES: protein motif:HMM:NF018685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR0903313.1"
                     /translation="MKRTRFWVGVLAAAALLSVGAILLLGRWSGGGHMARIYQDGVLI
                     REIDLDSLSAPLTFTVEFQGRTNTITAQPGRIRVESADCPDQVCVDQGWISDGTVPVV
                     CLPNRLVIQIEGGGDGLDSATG"
     gene            1074..1598
                     /locus_tag="ACZPAQ_04685"
     CDS             1074..1598
                     /locus_tag="ACZPAQ_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002848564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR0903314.1"
                     /translation="MVLTPPQDKRPSQARRLTRAALLTAIALTIFLAEAQLPPPVPIP
                     GIKLGLANIVTVYAMFVLGPRDTLLILLSRIFLGAVFSGQMMTLLYSLGGGLLSYLAL
                     LLLRRVLGRGQLWVSSPLSAVCHNLGQLLAAAAIAKSWVVLVYLPYLVLAGLITGTLT
                     GLCAQLVVGRLSRN"
     gene            1726..3126
                     /locus_tag="ACZPAQ_04690"
     CDS             1726..3126
                     /locus_tag="ACZPAQ_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0903315.1"
                     /translation="MNPMYDVVILGCGEAGIFAAYELARLHPDLKITALDQGRDIYHR
                     SCPIVAGKVKECIHCPVCDTMCGFGGAGAFSDGKFNFTTQFGGWLTDFMDAREVMELI
                     DYVDSINVAHGATTECFSTSTPEARALERRALAYDLHLLQARCKHLGTENNLRILQNI
                     YEGLKDKLEFRFNTPVTHIAREGEDFRLTLGKGEELSCRWLIAAPGRSGAEWFAEECK
                     GLGIHLINNQVDIGVRVELPAQVFEHITDVVYESKLVYRTKGYGDQVRTFCMNPYGHV
                     VAENVEGINTVNGHSYADPKLRSENTNFALLVSNRFTQPFNEPYRYGKHIASLSNMLA
                     GGVLVQRFGDLVKGVRTNEHRLGKSFTRPTLTAAVPGDLSLALPKRQLDDIIEMIYVL
                     DKIAPGTANHDTLLYGAEVKFYSSRLELSGELETSIPGFFAAGDGAGVTRGLAQAGAS
                     GVQAARAISRRISQQV"
     gene            complement(3357..>3586)
                     /locus_tag="ACZPAQ_04695"
     CDS             complement(3357..>3586)
                     /locus_tag="ACZPAQ_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903316.1"
                     /translation="GQTQDADSPRPGGVATAAMVSVVLSIRTILLPGGALRQETQRFL
                     QKIFLCKLGGKNPLTGVMKTAGLHTDSREST"
BASE COUNT          559 a         1244 c         1075 g          708 t
ORIGIN      
        1 cctatggccg ggagacccag ggggctctgg atatcaccgc ctactccgcc gttcatgcct
       61 ggggcttcac caccggcgag taccgtgtcc ccggaccgga ggagctggcg gagctggccg
      121 cccgcatcga ctacacccag gtggagctgg acccggaggg ctgccgggtc cgcctgcccc
      181 aggggatgca gctggatctg ggctccatcg ccaagggctg ggccagcgat ctgctggcgg
      241 agctggccgg cggggaaccc gccatcttca atctgggcgg caacgtgcgg accgcagggg
      301 acaagccgga cggctctccc tggcgcattg gcatccagac cccggaggcg gggagctccg
      361 gctacctggg ggtcctggag ctcaccggca gccaggcggt catcacctcc ggcggctatg
      421 agcgctactt tgaggaggac ggccagactt actggcacat catggacccg gagaccgccg
      481 ctcccgcccg gtccggcctg aagagcgtca ccatcgtctc ccctctgggg gtgtactgcg
      541 acgctctctc caccgccgtg ttcgtccggg gggcagactg gggggccgat ttttggcgga
      601 cccatcagga ctttgagatg gtgctggtgc tggaggacgg ttccctgtgg gtcactgccg
      661 gtctggctgg ttccttcacg ctggcagagg actatcagga cctggaggtg acggtgatcc
      721 agctatgaaa cgcacgagat tctgggtagg cgtgctggcc gccgccgccc tgctgtccgt
      781 cggcgccatc ctcctgctgg gccgctggtc cgggggcggg cacatggccc gtatctatca
      841 ggacggtgtc ttgatccggg agatcgatct ggacagtctc tccgcccctc tcacctttac
      901 agtggagttc cagggacgga ccaacaccat caccgcccag ccgggccgga tcagggtgga
      961 gtccgcggac tgcccggacc aggtctgtgt ggaccaggga tggatctccg acggcaccgt
     1021 ccccgtggtc tgcctgccca accggctggt cattcaaatc gagggaggag gggatggtct
     1081 tgactccgcc acaggataag cgcccctccc aggcccgccg cctcacccgg gccgcccttc
     1141 tcaccgccat cgccctgacc atctttctgg ctgaggccca gctgccccct ccggtcccca
     1201 tccccggcat caagctgggg ctggccaaca tcgtcaccgt ctacgccatg ttcgtcctgg
     1261 ggccccggga caccctgctc atcctcctct cccggatctt tctgggcgcg gtgttttccg
     1321 gccagatgat gaccctgctg tacagcctgg ggggcggcct gctcagctat ctcgccctgc
     1381 tcctcctgcg ccgggtgctg ggccggggcc agctctgggt cagcagcccc ctgtccgccg
     1441 tatgccacaa cctgggccag ctgctggccg ccgccgccat cgccaagagc tgggtggtgc
     1501 tggtctatct cccttatctg gtgctggccg ggctcattac cggcaccctc accggcctat
     1561 gcgcccaact ggtggtgggc cgcctgtccc ggaattgatc tccttccctc ctgtttttgt
     1621 ccaattccct tttctttttc tcatttctgt gctatcatag atgtgtctgt cctgttgggc
     1681 gggcctgcca tctgttttct actacactat gagaggagct ctcccatgaa ccccatgtac
     1741 gacgtcgtca tcctgggctg cggcgaggcg ggcatctttg ccgcctatga gctggcacgc
     1801 ctccatcccg acctgaagat caccgccctg gaccaggggc gggatatcta ccaccgcagc
     1861 tgccccatcg tggcgggcaa ggtgaaggag tgcatccact gcccggtgtg cgacaccatg
     1921 tgcggcttcg gcggggccgg ggccttctct gacggaaagt tcaacttcac cacccagttc
     1981 ggcggctggc tcaccgactt catggacgcc cgggaggtga tggagctgat cgactatgtg
     2041 gactccatca acgtggccca cggcgccacc accgagtgct tctccacctc cacccccgag
     2101 gccagggccc tggagcgccg ggcgctggcc tatgacctgc atctgctcca ggcccggtgc
     2161 aagcacctgg gcacagagaa caatctgcgc atcctccaga acatctatga ggggctgaag
     2221 gacaagctgg agttccggtt caacaccccc gtcacccaca tcgcccggga gggcgaggat
     2281 ttccgcctga ccctgggcaa gggtgaggag ctctcctgcc gctggctcat cgccgccccc
     2341 ggccgctccg gcgccgagtg gttcgccgag gagtgcaagg gccttggcat ccacctcatc
     2401 aacaaccaag tggatatcgg cgtacgggtg gagctgcccg cccaggtctt tgagcacatc
     2461 actgacgtag tctacgagtc caagcttgtc taccgcacca aagggtacgg ggatcaggtg
     2521 cgcaccttct gcatgaatcc ctacggccac gtggtggcgg agaacgtgga gggcatcaac
     2581 accgtcaacg gccactccta cgccgacccc aagctgcgca gcgagaacac caatttcgcc
     2641 cttctggtgt ccaaccgctt cacccagccc tttaacgagc cctaccgcta cggcaagcat
     2701 atcgcctccc tgtccaacat gctggcaggc ggcgtgctcg tccagcgctt cggcgatctg
     2761 gtgaagggcg tgcgcaccaa cgaacaccgg ttaggcaagt ccttcacccg gcccaccctc
     2821 accgctgccg tccccgggga cctgtccctg gccctgccca agcggcagct ggatgacatc
     2881 attgagatga tctacgtcct ggacaagatc gccccgggca ccgccaatca cgacaccctg
     2941 ctctacgggg cggaggtgaa gttctactcc tcccgtctgg agctgtccgg cgagctggag
     3001 acctccatcc ccggcttctt tgccgccggt gacggggcgg gcgtcacccg cggcctggcc
     3061 caggcgggcg cctccggcgt ccaggccgcc cgggccatca gccgccgcat ctcccagcag
     3121 gtctgagcat cccctcacag tcaggaccgc cgtattcgcc gcatggcgga cacggcggta
     3181 ttctttcatt ttttcctcgc tcttttttcc gctccgtccc tgtcctcact ccgcctccgg
     3241 attttttgac atgcggatat acagggcgat gcatacccct ggaaactgcc tctccctcct
     3301 tcttttccac cgccgggcgg gttttccaca gtttccacag ggttttccac agggtgttat
     3361 gttgactctc tgctgtccgt atgcagcccg gcggttttca taacgcctgt caaggggttt
     3421 tttcctccca gtttgcacaa aaatattttt tgcaggaacc gctgcgtttc ctgtcgaagc
     3481 gcgccgccag gaaggagtat cgttctgatg gagagaacca ccgacaccat tgccgccgtg
     3541 gccaccccgc cgggccgggg gctatcggca tcctgcgtct gtccgg
//
LOCUS       DBMDSJ010000150         3581 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6602_length_3581_cov_1.518966, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000150 DBMDSJ010000000
VERSION     DBMDSJ010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6602_length_3581_cov_1.518966"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="ACZPAQ_04700"
     CDS             complement(<1..555)
                     /locus_tag="ACZPAQ_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HIR0903317.1"
                     /translation="MSRLWVLLAAAALDWLLGDPAWMPHPIRLMGWTISRLETGLRRL
                     FPRTPGGELLAGGVLVLTMVLLFGGGSLLLLAALYALSPAAGTAAEVWLSYQLLAARC
                     LRDESMAVYRALTGRSLMDAQRAVGRIVGRDTQCLDRDGVTRAAVETVAENTSDGVIA
                     PLFWLALGGLPLGMVYKAVNTMDSM"
     gene            complement(552..1133)
                     /locus_tag="ACZPAQ_04705"
     CDS             complement(552..1133)
                     /locus_tag="ACZPAQ_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012521.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR0903318.1"
                     /translation="MELLMIRHSSTRGNLLRQYVGSQDHPLAPEGVALAERRRGEMPP
                     IDGLWVSPMLRCRQTAEILFPGVEQRLVDALKECDFGTFEGKTWAELKDDPIYQAWLA
                     GDPHIAFPGGEVLGEHIARCRRGVAHVVEQARALGMSRPGILAHGGTLMSAMSGFAVP
                     HQDFYSWLPSNCGGYLVRVEEGEDMAFTLIETL"
     gene            complement(1155..1481)
                     /locus_tag="ACZPAQ_04710"
     CDS             complement(1155..1481)
                     /locus_tag="ACZPAQ_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR0903319.1"
                     /translation="MILIVGGLGAGKRDFARRELGVREEELSPVLGPGTQVLYDLHAL
                     DPLPDLEELMGLRAVICNEVGCGLVPIQAEERQRREAVGRLCCQLAERAEAVYRISCG
                     LAIRLK"
     gene            complement(1478..2251)
                     /locus_tag="ACZPAQ_04715"
     CDS             complement(1478..2251)
                     /locus_tag="ACZPAQ_04715"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR0903320.1"
                     /translation="MRYLRAVSMAFSMYSRLPMPWVRWSDDCRAWVLCAFPLVGAVLG
                     LLLLAWSALADLLTLTPQLRAAGAVLLPLAYTGGIHMDGFCDTCDALASHQDRARKLE
                     ILKDSRVGAFAVLGCVSYLLLQYALWCQPGWEKEDLWVLALVPVLSRAMSGYGALTQP
                     GARADGLLAAFTEQEQGRGRQRFLLFLCALLALAIGMLGVGCAAVAAAILVYLWYIQM
                     ARREFGGLTGDLAGWFLQVCELGCLAAMVLAREIAEVLI"
     gene            complement(2248..2778)
                     /locus_tag="ACZPAQ_04720"
     CDS             complement(2248..2778)
                     /locus_tag="ACZPAQ_04720"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015563727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR0903321.1"
                     /translation="MEALVYGGAASGKSRYAEDLLCALSCEGPRIYLATMEAMDEESR
                     ARVRRHRALRRNKGFSTLECPRDLGGLTIPVGSSVLLEDLGNLWANELFSPSGFDPDG
                     ALLRTAEGLERLSVQAQNLVVVSADVFGEEVPCSPETAVYLDGLARLHRLAAGRCGRV
                     IEVVCGLPVIWKEECC"
     gene            complement(2780..>3581)
                     /locus_tag="ACZPAQ_04725"
     CDS             complement(2780..>3581)
                     /locus_tag="ACZPAQ_04725"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR0903322.1"
                     /translation="AASMAWGEACVCRMAWTAGAQVFPVDIGIAHPVEEGGLLRRHVT
                     RGTRSIAKGPAMTRDEAVAAVVTGITLAEELKGRGYRLLAAGEMGIGNTTTAAAVTAV
                     LLGRQVEEVTGRGAGLSDEGLERKIAVIRRAIEVNAPDPRDPLDVLSKVGGLDIAGMA
                     GFYLGAALHHVPVVLDGAISAAAALLAVRLCPDAAKALIASHRSAEPSSGLLLDALGL
                     RPIVSADLKLGEGTGAVTIMPMLDMAMSIYRDMATFQGLGIPPYRRQS"
BASE COUNT          650 a         1361 c         1066 g          504 t
ORIGIN      
        1 catggagtcc atggtattga cagctttgta caccatgcct aggggcagcc cgcccagagc
       61 cagccagaac aggggagcga tcacgccgtc agaggtgttt tctgccaccg tctccactgc
      121 ggcccgggtc acgccgtccc ggtccaggca ctgggtatcc cgtcctacaa tgcggccaac
      181 cgccctctgc gcgtccatga ggctcctgcc ggtcagggcc cgatacaccg ccatactctc
      241 atcccggagg caccgggcgg ccagcagctg atagctgagc cagacctccg ccgcagtccc
      301 cgccgccgga gagagggcat atagagcggc caggagcagc aggctccccc ctccgaagag
      361 caggaccatg gtcagcacca gcaccccgcc agccagcagt tctcctcccg gcgtcctggg
      421 aaaaagccgc cgcaggccgg tctccaggcg gctgatggtc catcccatca gccgtatggg
      481 gtggggcatc cacgccgggt cccccaggag ccagtccagc gcggcagcgg ccagaaggac
      541 ccacaggcgg ctcacagcgt ctcaatcagg gtgaaggcca tgtcctcccc ctcctccacc
      601 cggaccagat acccgccgca gttgctgggc agccagctgt aaaaatcctg atgtggcacc
      661 gcaaagccgg acatagcgga catcagggtc ccgccgtggg ccagaatccc gggccgggac
      721 atgcccaggg cacgggcctg ctccaccaca tgggccaccc ctcggcggca gcgggcgatg
      781 tgctccccca gcacctctcc tccgggaaag gcgatgtggg ggtcccccgc cagccacgcc
      841 tgatagatgg ggtcgtcctt cagctctgcc caagtcttgc cctcaaaggt tccgaaatcg
      901 cactccttca gcgcgtccac cagccgctgc tccacgccgg gaaacaggat ctcagccgtc
      961 tgccggcacc ggagcatagg tgagacccac agcccatcga tgggaggcat ctccccccgg
     1021 cggcgctccg ccagcgccac gccctccggg gccagggggt ggtcctggga gcctacatac
     1081 tgccgcagca gattccctct ggtggagctg tggcggatca tcagcaattc catgtctatt
     1141 cctctctgtc tgttctattt tagtcttata gccagcccgc aggagatccg gtacaccgcc
     1201 tcggccctct ccgccagctg gcagcagagg cggcccaccg cctcccgccg ctgccgttcc
     1261 tccgcctgga tggggaccag gccgcagccc acctcgttgc agatcactgc ccgcagtccc
     1321 atcagctcct ccagatccgg gagggggtcc agcgcatgga ggtcgtagag cacctgtgtc
     1381 ccaggcccca ggaccgggga cagctcctcc tcccggaccc ccagctcacg ccgggcgaag
     1441 tcccgttttc ctgccccaag gccgcccacg atcaggatca tatcaacacc tccgctatct
     1501 ctctcgccaa caccatcgcc gccagacagc ccaattcgca cacctgaagg aaccaccccg
     1561 ccaggtcacc cgtcaaaccg ccgaactccc gacgggccat ttggatatac cagagataga
     1621 cgaggatggc ggcggccaca gcggcgcagc ccacccccag catcccgatg gccagcgcca
     1681 gcagagcgca cagaaacagc aaaaaccgct gccgccccct cccctgctcc tgctccgtaa
     1741 aggcggccag caggccgtcc gccctggccc ccggctgggt cagcgccccg taccccgaca
     1801 tggcccggga gagcacgggg accagagcca gtacccacag atcctccttc tcccagcccg
     1861 gctggcacca cagggcgtat tgcaggagga gataggacac acatcccagc accgcgaagg
     1921 cccccacgcg ggagtccttc aggatctcca gcttccgggc ccggtcctgg tgggaggcca
     1981 gcgcatcaca ggtgtcgcag aagccgtcca tatggatacc acctgtgtag gccagcggca
     2041 ggaggaccgc ccccgctgcc cggagctgcg gcgtcagcgt cagcagatcc gccagcgcgg
     2101 accaggccag caggagcaga cctaggacag cccccacaag gggaaacgcg cacaaaaccc
     2161 aggcccggca gtcgtcggac caccggaccc agggcatggg aagacgggag tacatggaga
     2221 aggccataga gaccgcccgc agatacctca acagcactcc tccttccaga tcaccggcag
     2281 tccgcagacc acctctatta cccggccgca ccgccctgca gccagccggt gcaggcgggc
     2341 cagcccgtcc agatagaccg ccgtctcggg agaacagggg acctcctctc cgaacacatc
     2401 ggcgctcacc accaccaggt tctgggcctg gacgctgagc cgctccaacc cctccgccgt
     2461 ccggagcagg gccccatccg ggtcgaagcc cgaaggggaa aacagctcat tggcccacag
     2521 attgcccaga tcctccagca gcacgctgct ccccaccggg atggtcagcc cgcccaggtc
     2581 ccgcgggcac tccagggtgg agaagccctt gttccgccgc agggcccggt ggcgccggac
     2641 ccgcgcccgg ctctcctcat ccatcgcctc catggtggcc agatagatcc gaggcccctc
     2701 acaggacagt gcacacagaa gatcttccgc gtaccggctc ttgccgctgg ccgcgccgcc
     2761 gtaaaccagt gcctccatct cagctctggc ggcgataggg cgggatgccc aggccctgga
     2821 aggtggccat gtcccgatag atggacatgg ccatatccag catgggcatg atggtcacgg
     2881 cgccggtccc ctctcccagc ttcaggtccg ccgaaacaat agggcgcagc cccagggcgt
     2941 ccaggagcag gccgctggag ggctccgctg acctgtggct ggcgatgagg gccttggccg
     3001 cgtccgggca gagccgcaca gccagcaggg cggccgcggc ggagatggcg ccgtccagta
     3061 ccacaggcac atggtggagg gcggccccca gataaaagcc cgccataccg gcgatatcca
     3121 gccctcccac cttggagagc acatccagcg ggtcccgggg gtcaggggcg ttgacttcga
     3181 tggccctgcg gatcacggcg atcttccgct ccagcccttc atctgacagg ccggcgcccc
     3241 ggcccgtcac ctcctccacc tgccgcccca gcagcactgc tgtcacggcg gcggcggtgg
     3301 tggtgttgcc gatgcccatc tcccctgccg ccaacaggcg atatcccctg cccttgagct
     3361 cctctgccag cgtgatgccg gtgaccactg cggccaccgc ctcgtcccgt gtcatggcag
     3421 ggcccttggc gatgctcctg gtcccacggg tcacgtggcg gcgcagcagg ccgccctcct
     3481 ccaccgggtg ggcgatcccg atgtccaccg ggaacacctg tgctccagcg gtccaggcca
     3541 tgcggcagac acaggcctcc ccccaggcca tggaggccgc c
//
LOCUS       DBMDSJ010000151         3547 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6664_length_3547_cov_1.574869, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000151 DBMDSJ010000000
VERSION     DBMDSJ010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6664_length_3547_cov_1.574869"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            297..1067
                     /locus_tag="ACZPAQ_04730"
     CDS             297..1067
                     /locus_tag="ACZPAQ_04730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR0903323.1"
                     /translation="MDLHKCLLTENDCYKAGRKIVPKGVMVHSTGANNPNLRRYVGPD
                     DGLLGQNPNSNHWNMSGVGACVHAFIGKMADGSVATYQTLPWTARGWHCGTGSKGISA
                     NNTHISFEICEDGLKDRDYFDLVYREAVELTAYLCREYGLDPLEDGVVICHQEGARRG
                     IASNHADVLHWFPMHGMTMDDFRADVAQEMEAETVTYEQWLEYMERYRREMAAEKPAM
                     PELLAEAVELNLTDGSRPRDLMTREEGAIMARAAAKAR"
     gene            1473..2006
                     /locus_tag="ACZPAQ_04735"
     CDS             1473..2006
                     /locus_tag="ACZPAQ_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR0903324.1"
                     /translation="MGFIQDEMDLKLLVLYIMARIAAPITFLQLLELALCDAGVDYFS
                     LTQAVEHLVETEHLSREGERYAITEKGRRNSEICESSLPYSVRRRCDDNLVRVNETLM
                     REQQVQGEVLPNPDGTCTVRLRLADDSGPLLELSLLMPAAGQGERAAARFKQAPEQLY
                     HQLVQLLSVPEEREETP"
     gene            2175..2684
                     /gene="nusB"
                     /locus_tag="ACZPAQ_04740"
     CDS             2175..2684
                     /gene="nusB"
                     /locus_tag="ACZPAQ_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR0903325.1"
                     /translation="MTRSNAREVAVHMIYELGFGSRSAEELLEHEMTQERFAQLGEEE
                     PLYAQFPNEKQRQYIRDLVQGVYVHGPELDDYISRYSVGWSFARIPRMAAAVMRTAMY
                     EVLYMPAIPNSAAINEAVEIAKNYEPAEVVSFINGILGSFVRAEFADSAPKPEKKEEE
                     AAGPEEPEE"
     gene            2681..>3547
                     /locus_tag="ACZPAQ_04745"
     CDS             2681..>3547
                     /locus_tag="ACZPAQ_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kae1-like domain-containing protein"
                     /protein_id="HIR0903326.1"
                     /translation="MTMPVLGLDTSNYTTSAAVFDGADGCNAGRLLAVRPGELGLRQS
                     DALFQHVKQLPALLEELEGRGLLRDLKAVGASTRPRAVEGSYMPCFLAGESQGRALAA
                     ALGVPFFACSHQQGHLAAAAWSAGRTDLLDGPFLAWHLSGGTTELLLVRPQGTNVAAE
                     KLGGTSDISAGQLIDRTGVLLGLQFPAGKALDELYPQADACMEYRVKLKDLTFSLSGM
                     ENQVKRLLTEGEAPANVARFTLDTVCSVVLRATQEAQRRCPGLPVLCSGGVASNRQLR
                     AAMERSCGALFAQ"
BASE COUNT          695 a         1004 c         1232 g          616 t
ORIGIN      
        1 cctgtggtcc tattaccgct ttgccatcac cggctggatg atcgtggcgc tgatcgccct
       61 catcttcctg gtggccttct ccgcaatgtt cggttatgac aaactggtgc agctgatgga
      121 gcaggcaggg tggatcaagg cacagaaata aaaaagcacc cgcgtagcgg gtgcctgggc
      181 cctgcttggc ttcgttcggc tccggcagtt acgcagggag ctctatgacg catgatgcgg
      241 tgatcgcacg atgcagggcc aaatcatact accatactga gacaggaggt gtcaacttgg
      301 atctgcacaa gtgcctgttg acggagaacg actgctacaa ggccggacgg aagattgtac
      361 ccaagggggt catggtccac tccaccgggg ccaacaaccc caatctgcgc cgatacgtag
      421 ggccggatga cggcctgctg ggccagaatc caaacagcaa ccactggaat atgagtgggg
      481 ttggggcttg cgtccatgct ttcatcggca agatggctga tggctctgtg gccacctatc
      541 agacactgcc ctggacggcc cggggatggc actgcggaac agggagcaag ggcatcagcg
      601 ccaacaacac ccacatcagc tttgagatct gtgaggatgg gctgaaggac cgggactact
      661 ttgacctggt atatcgggag gcggtggagc tgacggccta cctgtgccgg gaatacggtc
      721 tggacccgct ggaggacggg gtggtgatct gccaccagga gggagcccgc cgggggatcg
      781 ccagcaatca cgctgacgtg ctccactggt tcccgatgca cgggatgacc atggatgact
      841 ttcgggccga tgtggcccag gaaatggagg ctgagactgt gacctacgag cagtggctgg
      901 agtacatgga gagatatcgc agggagatgg ctgccgaaaa gcctgccatg ccggagctgt
      961 tggcggaggc cgtggagctg aacctgacag acggcagccg cccccgggat cttatgaccc
     1021 gagaggaagg cgcaattatg gcccgggcag cggcgaaggc cagatagaca gaagaaaatt
     1081 tgtttataaa gcgctatatc ttcatctttg caggaatcaa aaattttttt cgtgtgtatg
     1141 cacacggcct cccggatgca gcgatcatcg ctcacgggac cgggcgcgac cttttcaggc
     1201 atatggatac ctcctcgtca gattgagtgg agtggacggg cacgggggtg ccgggcccgc
     1261 cggaagtgaa gggcttccat accatactat gaccgacagg cgggatggtg ccagaggagc
     1321 cggaaatgcg gggttgtcct ttcgcgcgtt ttgttctata ataaaataga aagaccatgg
     1381 gcacgttccc accaaaggtg gagcggtgtc cgggacgccg ccggcccgcc agggccgggg
     1441 caagaagaga gaggtgtgct tggatggccc ggatgggatt tattcaggat gagatggatc
     1501 tgaagctgct ggtcctgtat attatggccc gcattgcggc gccgatcacg ttccttcagc
     1561 ttctggagct ggcgctgtgc gacgccgggg tggactattt ctccctgacg caggcggtgg
     1621 agcatctggt ggagacagag cacctgagcc gggaggggga gcgctacgcc atcacggaga
     1681 aggggcggcg caacagcgag atctgtgaga gcagcctgcc atactcggtg cgccgccggt
     1741 gcgacgacaa tctggtgcgg gtgaatgaga ccctgatgcg ggagcagcag gtgcaggggg
     1801 aggtgctccc aaacccggac gggacgtgca cggtgcgact gcggctggcg gacgacagcg
     1861 gccccctgct ggagctgagc ctgctgatgc ccgcggcggg acagggggag cgggcggccg
     1921 cccgcttcaa acaggcgccg gagcagctct accaccagct ggtccagctg ctcagcgtcc
     1981 cggaggagcg ggaggagacg ccgtgaggcg cagcgccccg gaacccggga gaaaaatccc
     2041 gtaaaacata ttttagggtt ttctttttgt atatacatgt atataatgga tctggatgaa
     2101 aagtcggagc cccggagacg cgggccagag agaaagattg ggacagaggg cccaagtgac
     2161 ggagggatca cacaatgacg agaagcaacg cccgtgaggt cgcggtgcat atgatctatg
     2221 aactgggctt tggcagccgc agcgcggagg agctgctgga gcacgagatg acccaggagc
     2281 ggttcgcaca gctgggggag gaggagcccc tctatgccca gtttcccaac gagaagcagc
     2341 ggcagtacat ccgggatctg gtacaggggg tctatgtcca cgggccggag ctggatgact
     2401 acatctcccg ctattccgtg ggctggagct ttgcccgcat cccccgcatg gcggctgcgg
     2461 tgatgcgcac cgccatgtat gaggtgctgt atatgccggc gatccccaac tccgccgcca
     2521 tcaacgaggc ggtggagatc gccaagaact atgagccggc cgaggtggtc tcctttatca
     2581 acggcatcct gggctccttt gtccgggcag agtttgccga ttccgccccc aagccggaaa
     2641 agaaggagga ggaagcggcg ggaccggagg agccggagga atgaccatgc cggttctggg
     2701 actggatacc agcaactata ccacctccgc agccgtcttt gacggggcgg atggctgcaa
     2761 cgccggccgc ctgctggcgg tgcggcccgg agagctggga ctgcggcaga gcgacgccct
     2821 gttccagcat gtgaagcagc tcccggcgct gctggaggag ctggagggcc gcggactgct
     2881 ccgggacctg aaggcagtgg gggccagcac ccggccccgg gctgtggagg gctcctacat
     2941 gccctgcttc ctggcggggg agtcccaggg aagggcgctg gccgccgccc ttggggtgcc
     3001 gttttttgcc tgctcccacc agcaggggca cctggcggcg gcggcctggt ccgccgggcg
     3061 gacggacctg ctggacggtc ccttcctggc ctggcacctg tccggcggca ccaccgagct
     3121 gctgctggtc cggccccagg ggaccaatgt ggcggcggag aagctgggcg ggaccagcga
     3181 catctccgct ggccagctca tcgaccgcac cggcgtcctg ctggggcttc agttcccagc
     3241 tggcaaagcg ctggacgagc tctatcctca ggcggatgcc tgtatggagt accgcgtgaa
     3301 gctgaaggac ctcacgttct ccctgtccgg gatggagaat caggtgaaga gactgctgac
     3361 ggagggagag gccccggcca acgtggcccg cttcaccctg gacacggtgt gcagcgtggt
     3421 cctccgggcc acccaggagg cccagcgccg ctgccccggc ctgccggtgc tgtgctccgg
     3481 cggtgtggcc tccaaccgac agctgcgggc cgccatggag aggagctgcg gcgccctgtt
     3541 cgcccag
//
LOCUS       DBMDSJ010000152         3546 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6666_length_3546_cov_1.379681, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000152 DBMDSJ010000000
VERSION     DBMDSJ010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6666_length_3546_cov_1.379681"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="ACZPAQ_04750"
     CDS             complement(<1..371)
                     /locus_tag="ACZPAQ_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR0903327.1"
                     /translation="MVHTFSALGRNLAVDVNSGAVHVLDKLCCDLLSLVEGPMGDACP
                     QDLKDRLSQYDARELDEAWTELLELQRAGLLFSDDDYIDPAAAMALPKAAVVKALCLH
                     VSHDCNLRCRYCFASTGDFGTG"
     gene            complement(469..615)
                     /gene="scfA"
                     /locus_tag="ACZPAQ_04755"
     CDS             complement(469..615)
                     /gene="scfA"
                     /locus_tag="ACZPAQ_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR0903328.1"
                     /translation="MKHIQTLNGATLKNSAAHGGCGECQTSCQSACKTSCTVANQKCE
                     NDNK"
     gene            complement(710..1129)
                     /locus_tag="ACZPAQ_04760"
     CDS             complement(710..1129)
                     /locus_tag="ACZPAQ_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HIR0903329.1"
                     /translation="MKTKGKREEAESPWLGTMCGMLKGGALAFLTAVAILFLCALAVS
                     NRWLGQGMMDSAVMAACALGALAGGLLAVRQGRGRSLLMGFGAGLILFLLLLTAGFLF
                     YESADLRNGGLGLLLSCGCGGALAGVLGGKPKKKRRR"
     gene            complement(1245..1517)
                     /gene="yajC"
                     /locus_tag="ACZPAQ_04765"
     CDS             complement(1245..1517)
                     /gene="yajC"
                     /locus_tag="ACZPAQ_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR0903330.1"
                     /translation="MYSIVLLVLMFALLYFFMIRPENKRKKQAQAMRDSLTVGDEITT
                     IGGIIGTICAVKENTIVIETGADRVRIEFTKWAVSTKGAQTSETTK"
     gene            complement(1651..2316)
                     /locus_tag="ACZPAQ_04770"
     CDS             complement(1651..2316)
                     /locus_tag="ACZPAQ_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR0903331.1"
                     /translation="MADRLLFSKTGRAKYISHLDLMRTFQRAFFRSGIQIRHTEGFNP
                     HPFVSIALPLSVGYSSQCEILEFGLVGGASYEEVPARLTAAMPEGIVIHSCYPAQRPV
                     KELSRVNYIINMEYEEGRPLEAETALSRLLGQESLVVRKKSKKAKAGFTEVDLIPLIH
                     SWNLEVQRDTMTLDAVLAAQNPGLNPELIRAAFCETYPDLQPDFVTFHRRAVLDGEGK
                     EFR"
     gene            complement(2309..>3546)
                     /locus_tag="ACZPAQ_04775"
     CDS             complement(2309..>3546)
                     /locus_tag="ACZPAQ_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR0903332.1"
                     /translation="SFYEPVYRPDGTLEEMRIREGSGAPELVRKRVVEDMNKSYFLVK
                     TIIPSTEIVHDRVMLELFRGCIRGCRFCQAGYVYRPVRSRDPKQLAEYGKAACEDSGY
                     QEMTLSSLSSTDYPCLLELCDDLLDYCAPRDIGLSLPSLRADTFSMNLMERLQRVRKG
                     GLTFAPEAGTQRLRDAINKNLREEDLLQSCRTAFAGGWSGVKLYFMLGLPTETDEDVL
                     GIADLARKVYFAWREYTPNPKRGVRITVSTSWFVPKPHTAFQWEAQIPVEEYQRRVGL
                     LRDALKRDKSITYNWHDPQTSYLEAIFSRGDRRLADVLEWAWQHGAKFDSWSEYFDYQ
                     RWMDALEACGLDGDFYAHRERPRSEVFPWCRIDPMVTPAFLWHERELCYQSQTTPDCR
                     TRCSGCGANRLLKGGVCNG"
BASE COUNT          675 a         1160 c         1047 g          664 t
ORIGIN      
        1 ccggtaccga agtcgccagt agaggcaaag cagtagcggc agcgcaggtt gcagtcgtgg
       61 gagacatgca gacacagtgc cttcaccacc gccgccttgg gcagggccat ggcggcagct
      121 gggtcgatgt agtcgtcatc tgagaagagc agccccgccc gctggagctc cagcagctcc
      181 gtccaggcct cgtccagttc cctggcgtcg tactgagaga ggcggtcctt cagatcctgg
      241 ggacaggcat ctcccatggg gccctccacc agagaaagca ggtcacagca cagcttgtcc
      301 agcacatgga ccgccccgct gttcacatcc accgccaagt tccggcccag cgcggaaaat
      361 gtgtgtacca taatgatatt cctccgaatt tgcaaagatt ggcggccgat tccagccgcg
      421 aaaaaagggc aggctgacgc ccgccccttt ccggtcagga tgttgtcctt acttgttgtc
      481 gttctcgcac ttctgattgg cgaccgtgca ggaagtcttg caggcggact ggcaagaggt
      541 ctggcactcg ccgcagccgc cgtgggctgc gctgttcttc aaagtggctc cgttcagagt
      601 ctggatgtgc ttcatcggaa tcttcctccc taatatgctg tcatagggcg gagcatggcc
      661 ccgccaatat gtatggtagg attataccat acacaggccg ggatttcaat catcttctgc
      721 gttttttctt tggttttccc cccagtacgc ccgccagggc accgccgcag ccgcaggaga
      781 gcagcagtcc cagcccgccg ttgcgcagat ccgctgactc atagaacagg aaaccggcag
      841 tgagcagcag caggaacagg atcagacctg ccccgaagcc catcagcagg ctcctgcccc
      901 ggccctgacg gacggccagc aggccgccgg cgagagcccc cagggcacag gccgccatca
      961 ctgcgctgtc catcatcccc tgccccagcc agcggttgga caccgccagc gcacacagga
     1021 acaggatggc caccgccgtc agaaacgcca gtgcgccccc cttcagcatc ccgcacatgg
     1081 tccccagcca ggggctctcc gcctcttccc gctttccttt cgttttcaca aaaaacaccc
     1141 tccccgcaag ttccaggtat ggaatcttat gcgggaaggg tgcttgtcat gcctgtcatt
     1201 tctgccccag ggcacagcag cccggaagca cggcacggag tcctttactt cgtggtctcg
     1261 gaagtctggg cccccttggt ggacacggcc cacttggtga attcgatccg cacacggtcg
     1321 gcaccggtct cgataacgat ggtgttctcc ttcacagcac agatggtgcc gatgatgccg
     1381 ccgatggtgg tgatctcgtc cccaacggtc agagagtccc gcatggcctg cgcctgcttc
     1441 ttccgcttgt tctccgggcg gatcataaag aagtagagca gcgcaaacat caggactaac
     1501 aaaacaatgc tgtacatccc actatccaag gtagattccc tccaaaaatt cggtagtgct
     1561 gccaaaaggc gtctttgtca ttatacctgt ttccccttcg gttgacaagc cttttttgca
     1621 aattttctca aacctggttt tacacgccgg tcagcggaac tcctttccct ccccgtccag
     1681 caccgcgcgg cggtggaagg tgacgaaatc cggctggaga tctgggtaag tctcgcaaaa
     1741 agccgcccgg atcagttctg gattcagtcc cggattctgg gccgccaaca ccgcatccag
     1801 ggtcatggtg tcccgctgca cctccaggtt ccagctgtgg atcagaggga tcaggtccac
     1861 ctcggtgaag ccggccttgg ccttcttcga cttcttgcgc accaccaggc tctcctgccc
     1921 cagcaggcgg ctcagggcgg tctctgcctc caaaggccgc ccctcctcgt actccatatt
     1981 gatgatgtag ttgacccgag acagctcctt caccggccgc tgggccggat agcagctatg
     2041 gatcacgatg ccctccggca tggcggcagt gagccgcgcc ggcacctcct cataggatgc
     2101 gccccccacc agcccgaact ccaggatctc gcactggctg gagtagccca ctgacagggg
     2161 cagcgcgatg gagacgaagg ggtggggatt gaagccctcc gtgtgcctga tctggatgcc
     2221 ggagcggaaa aaggcccgct ggaaggtgcg catcaggtcc agatgggaga tgtacttggc
     2281 tctcccagtc ttggagaaga gcaggcggtc agccattgca cacccctcct ttcagcagac
     2341 ggttggcccc gcagccggaa caacgggtgc ggcagtccgg ggttgtctgg gactggtagc
     2401 acagctcccg ctcatgccac aggaaagcgg gggtcaccat ggggtcgatg cggcaccagg
     2461 gaaacacctc gctgcgcggc cgctcccggt gggcgtagaa gtccccatcc aacccgcagg
     2521 cctccagggc atccatccac cgctggtagt cgaaatactc gctccaggag tcgaacttgg
     2581 ccccatgctg ccaggcccac tccaacacgt cggccaggcg gcggtccccc cgggagaaga
     2641 tagcctccag ataactggtc tgggggtcgt gccagttgta ggtgatggac ttgtcccgct
     2701 tcagggcgtc ccgcagcagg cccacccgcc gctggtactc ctccacgggg atctgggcct
     2761 cccactggaa ggccgtgtgg ggcttgggca cgaaccagga ggtggacacc gtgatccgca
     2821 ccccccgctt ggggttggga gtgtactccc gccaggcgaa atagaccttg cgggccagat
     2881 ccgcgatccc cagcacatcc tcatcagtct cggtgggcag gcccagcatg aagtagagct
     2941 tcacgccgct ccacccgccg gcgaaagcgg tgcggcagga ttggagcagg tcctcctccc
     3001 gcaggttctt gttgatggcg tcccgcagcc gctgggtccc cgcctccggc gcgaaggtga
     3061 ggcctccctt cctcacccgc tggagccgct ccatcagatt catggagaag gtgtccgccc
     3121 gcagggaggg cagtgagagc ccgatgtccc ggggggcgca gtagtccagc aggtcatcgc
     3181 acagctccag caggcaggga tagtcggtgg aggacaaaga agacagggtc atctcctgat
     3241 agccggagtc ctcacaggcc gcctttccat actctgcgag ctgtttgggg tcccggctgc
     3301 gcaccgggcg gtacacgtat ccggcctgac agaaccggca gccccggata cagccccgga
     3361 acagttccag catcacccgg tcgtggacga tctctgtaga ggggatgatg gtcttcacca
     3421 ggaaatagga cttgttcatg tcctctacca cccgcttgcg caccagctcc ggcgcgccgg
     3481 agccctcccg gatgcgcatc tcctccaggg ttccgtcggg gcggtagacc ggctcgtaga
     3541 aggagg
//
LOCUS       DBMDSJ010000153         3518 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6722_length_3518_cov_1.897571, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000153 DBMDSJ010000000
VERSION     DBMDSJ010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6722_length_3518_cov_1.897571"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(112..261)
                     /locus_tag="ACZPAQ_04780"
     CDS             complement(112..261)
                     /locus_tag="ACZPAQ_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903333.1"
                     /translation="MKQHILTTASFYFTNSWARFMSTGYFVLGKYREMEWMCRSQSGT
                     AALSV"
     gene            complement(373..1101)
                     /locus_tag="ACZPAQ_04785"
     CDS             complement(373..1101)
                     /locus_tag="ACZPAQ_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903334.1"
                     /translation="MKGIRAAVLALGGAFLLAGCGGESGVLAPIPAEGFSQGVYDALE
                     ENWTAWEAVDELERMLSSTLPGHCAQEFDSWAQCADFLGPSIPDPAADCGWLEEATYA
                     GMPLEASDAPRVKAEWYGTREGQVEWVSVQSGWRDGAVRVMVSAALYGTRAEDMPSYK
                     GWGTELARQAYLEAAEAGTPVVTAESGASWCSIQAEHARGSVLYTIRAVGESGTEEQV
                     RTSLDRVLETWLLAPEGEKTSKIC"
     gene            1389..1847
                     /gene="rimI"
                     /locus_tag="ACZPAQ_04790"
     CDS             1389..1847
                     /gene="rimI"
                     /locus_tag="ACZPAQ_04790"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR0903335.1"
                     /translation="MEYIIVPMDRGHIPQIAALERECFSTPWSENMLSDALFDPKASF
                     IVAEDGEGGVLGYAGLQVVLDEGYIDNIAVEEAARRHGVGDALLDVFCRFGQAHLSFL
                     TLEVRASNAPAIALYRKHGFQEAGRRKNYYQQPREDAIIMTREFEHHEAE"
     gene            1834..2463
                     /locus_tag="ACZPAQ_04795"
     CDS             1834..2463
                     /locus_tag="ACZPAQ_04795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903336.1"
                     /translation="MKLNELSPQQLAQLYRQELTSAFPPEELKPLRSMLSLMEQGRYQ
                     ALGLYDGEDLVAYALIWLEPGCPFALLDYLGTMAGLRDRGLGGRMLDLLAEHYAHFRG
                     IFGEAEAPENGDPAGEPLRRRRLAFYQRNGFRYGGYDCALFGVHYRALIRGAEDVTAE
                     ELLQVHQSIYRRHFPPALYQRFMQIPLRPGERPNPPGRWMEEEKDGVSQ"
     gene            2460..3470
                     /gene="tsaD"
                     /locus_tag="ACZPAQ_04800"
     CDS             2460..3470
                     /gene="tsaD"
                     /locus_tag="ACZPAQ_04800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR0903337.1"
                     /translation="MNILAIESSCDETAAAVVRDGRTVLSNCVASQIEMHTIYGGVVP
                     EIASRKHVEAVSGLAREALERAGLSREELDGVAVTYAPGLIGAVLVGVNFAKGAALAL
                     DRPLIPVHHVRGHIAANYLTHPDLEPPFVCLCVSGGTTAIVDVRSYTEMEVLGGTRDD
                     AAGECFDKVARVLGIGYPGGAPMDRLADGGDDAKYELPRAHVQGAELDMSFSGLKTAC
                     LNLIHNAQQKGDALDLPSFAASFGRAVSESLVPRAMEASRRKGYGRLAVAGGVAANRR
                     IRADLEAACRQSGDRLFLPELKYCGDNAAMIGCQGYYEFLAGRTAGLDLNAYATRDIS
                     LG"
BASE COUNT          587 a         1218 c         1042 g          671 t
ORIGIN      
        1 aggctcgatc atcactgtca tatgttccgc tccttttctc gctgtgctgc aaaaaaacgg
       61 gggacacacc gatgaaatcg atgtgtcccc cgcatcagca tccagatctg atcacacgga
      121 gagggccgcc gtcccactct gggaccggca catccattcc atctctctgt attttcccag
      181 cacgaaatag ccggtgctca taaatcgagc ccagctgttg gtaaaataga aagatgcagt
      241 tgtgaggatg tgctgcttca tgtggcacct cactgaaaat cattttttct tagtttaacc
      301 cgataaagtg aaaattgcaa tcgccgtttc atccataatt ccagattata atcactaaat
      361 ttttgtatgt atttaacata ttttagatgt tttttcccct tccggggcca aaagccaggt
      421 ctccagcacc cgatccagag acgtccgcac ctgctcctcc gtcccagact cccccaccgc
      481 gcggatggta tagagcacgc tgcctcgggc atgctccgct tggatggagc accagctcgc
      541 cccgctctct gcggtcacca ccggcgtccc cgcctccgcc gcctccagat aggcctgccg
      601 cgccagttct gtcccccagc ccttgtacga gggcatgtcc tccgcccggg tcccgtacag
      661 ggccgcggat accatcaccc ggactgctcc gtcccgccag cccgactgga cgctgaccca
      721 ctccacctgg ccctcccgtg tgccgtacca ctcggccttc acccgcgggg cgtcggaggc
      781 ttccaggggc atgcccgcat aggtggcctc ctccagccag ccgcagtccg ccgccgggtc
      841 cgggatggac ggccccagaa aatccgcgca ctgcgcccag ctgtcaaact cctgggcgca
      901 gtggcccgga agtgtgctgc tcagcatgcg ttccaactcg tccaccgcct cccaggccgt
      961 ccagttctcc tccagtgcgt cgtacacccc ctgagagaag ccctctgccg ggatgggcgc
     1021 cagaacgccg ctctccccac cgcatccagc cagtaggaag gcgcccccca gtgccaggac
     1081 cgccgctctg attcccttca tctccaccga tcccttcttc tctgtggttt ggctgtattt
     1141 taccatatga gatacccccg caacaagccc gtctttcctg gcggaggcct cccggcatcg
     1201 tgtcctgctt agtgcgcatg gctcccgggc cccctctcta cgccctgcag caccgatcat
     1261 actggactgg cgggctttgc gccgccggtc ctcttgaaaa gccccccctt ctccctttac
     1321 aaaacgcctg cctctggtat atgataatat gacatctacc cgtcacaccg ggagaacagg
     1381 aggaccctat ggaatacatc atcgtcccca tggaccgggg ccatatcccc cagatcgcgg
     1441 cgctggagcg ggagtgcttc tccacgccct ggagcgagaa catgctctcg gacgcgctct
     1501 ttgaccccaa ggccagcttc attgtcgccg aggacgggga gggcggcgtg ctgggctacg
     1561 ccggtctcca ggtggtgctg gacgagggct acatcgacaa catcgccgtg gaggaggccg
     1621 cccgccgtca cggggtgggg gatgcccttc tggacgtgtt ctgccgcttc gggcaggccc
     1681 atctctcctt cctgaccctg gaggtgcggg cctccaacgc ccccgccatc gccctgtacc
     1741 gcaagcatgg ctttcaggag gcgggccgcc ggaaaaacta ctaccagcag ccccgggagg
     1801 acgccatcat catgacaagg gagtttgaac accatgaagc tgaatgagct ctccccccag
     1861 cagctggccc agctgtaccg tcaggagctg acctccgcct ttcccccaga ggagctcaag
     1921 cccctgcgct ccatgctctc cctcatggag cagggacgct accaggccct gggcctgtac
     1981 gacggggagg atctggtcgc ctacgccctg atttggctgg agccgggctg tccctttgcc
     2041 ctgctggact acctgggcac catggccggc ctgcgggacc ggggcctggg cggccggatg
     2101 ctggacctgc ttgcggagca ctacgcccac ttccggggca tcttcgggga ggcggaggcc
     2161 cctgaaaacg gggaccccgc cggggagccc ctgcgccgca ggcggctggc cttctatcag
     2221 cgcaacggct tccgctacgg cgggtacgac tgcgccctgt tcggcgtcca ctaccgggcc
     2281 ctgatccgtg gggcggagga tgtgacggct gaggaactgc tccaggtcca tcagtccatc
     2341 tatcgccggc acttcccgcc ggcactctat cagcgcttca tgcagatccc cctccggccc
     2401 ggggagcggc ccaatccccc cggacgctgg atggaagagg aaaaggacgg tgtttcccaa
     2461 tgaacattct ggccattgag tcctcctgtg acgagaccgc cgctgcggtg gtgcgggacg
     2521 gcaggactgt gctgtccaac tgtgtggcct ctcagatcga gatgcacact atctacggcg
     2581 gcgtggtgcc cgagatcgcc tcccgcaaac atgtggaggc agtgagcggt ctggcccggg
     2641 aggccctgga gcgggccggc ctgagccggg aggagctgga cggtgtggcg gtgacctatg
     2701 ccccgggcct gatcggtgcg gtgctggtgg gggtcaactt tgccaagggg gccgcgctgg
     2761 ccctggaccg gcccctgatt cctgttcacc atgtgcgggg ccacatcgcc gccaactacc
     2821 ttacccaccc ggacctggag ccccccttcg tctgtctgtg cgtctccggc ggcaccaccg
     2881 ccatcgttga tgttcgctcc tatacagaga tggaggtgct gggcgggacc cgggacgacg
     2941 ccgccgggga gtgcttcgac aaggtggcca gggtgctggg catcggctat cccggcggtg
     3001 cccccatgga ccgcctggct gacggggggg acgacgccaa atatgagctg ccccgggccc
     3061 acgtccaggg ggccgagctg gacatgtcct tctccggcct gaagaccgcc tgcctgaacc
     3121 tgatccacaa cgcccagcag aagggggatg ccctggacct gccctccttt gccgcctctt
     3181 tcggccgggc ggtgagcgag agcctggtcc cccgggccat ggaggcctcc cgccggaagg
     3241 gatacggcag gctggcggtg gccggagggg tggcggccaa ccgccgcatc cgggccgacc
     3301 tggaggctgc ctgccggcag agcggtgacc gcctcttcct cccggagctc aaatactgcg
     3361 gcgacaacgc tgccatgatc ggctgccagg ggtactacga atttctggcc ggccgcactg
     3421 cgggcctgga cctgaatgcc tatgccactc gggacatctc cctgggatga cacctttttt
     3481 gtcattatgt cacctgcgtt tcgtttaccg gccctcct
//
LOCUS       DBMDSJ010000154         3507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6755_length_3507_cov_1.392249, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000154 DBMDSJ010000000
VERSION     DBMDSJ010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6755_length_3507_cov_1.392249"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /locus_tag="ACZPAQ_04805"
     CDS             <1..115
                     /locus_tag="ACZPAQ_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HIR0903338.1"
                     /translation="RVEFDQPQRAVTPGQAVVFYDGDLVIGGATIQKGRES"
     gene            112..426
                     /locus_tag="ACZPAQ_04810"
     CDS             112..426
                     /locus_tag="ACZPAQ_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903339.1"
                     /translation="MSKKRSQRKNSNYNYRPGQKAGEDPQALQENSRTKKFSPAARNL
                     LFVDLIFLAVCQMMESGGLLDDVLSAWLTLVGLILLLVAVGIQFQFWRGPKSKIPKGP
                     RS"
     gene            434..1000
                     /locus_tag="ACZPAQ_04815"
     CDS             434..1000
                     /locus_tag="ACZPAQ_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HIR0903340.1"
                     /translation="MKREYRRSEPLFSQCGLNCGLCPMYHIREESHCPGCGGPGRPSC
                     PILRCAGEHGGVEFCSLCPDWPCQRYVQEQADSFIPHRNARRDLETVKRVGLEHYRET
                     LGKKMEGLRFLLERCNDGRRKSLFCTAVNLLELEDVEAVIARLEEEFGPEYSPEEQKE
                     RGSRAAELFRERAQTRGVSLKLERQRKP"
     gene            1036..1797
                     /locus_tag="ACZPAQ_04820"
     CDS             1036..1797
                     /locus_tag="ACZPAQ_04820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012059059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0903341.1"
                     /translation="MNIQWEAGKYTENFDFVHRYGESVLELVEAEAGGLVVDLGCGNG
                     ALTQKLADRGYRVLGVDDSAEMVAEARALHPGLPFQKGDAVTFQLEEKADVIFSNAVL
                     HWIDGSRQQALARNVAAQLKPGGQFIFEFGGKGCAERVHSSLERQFARRGLAYPRTFY
                     FPTIGEYAPILERAGFRVEYAVLFDRPTEQKTGDGLADWVRMFVKKPFEGLEPALGDE
                     LLRAAVEELRPALFRDGKWFVDYVRIRMKARKTAG"
     gene            2002..2436
                     /locus_tag="ACZPAQ_04825"
     CDS             2002..2436
                     /locus_tag="ACZPAQ_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903342.1"
                     /translation="MKLKLILLGSLSPLIGLLIFFLQLQVPFHGIKYLVILAIMSAVF
                     LAYFFFCAFFFSSKQHKKNSLYFFVTPFILFLVNFIGWIQKYVVLALIVSGVSAPFHY
                     VLPDLIFPGDKYHLIMSIFPLVICWIAFKIGERVGIYFKERI"
     gene            complement(2646..>3507)
                     /gene="cgtA"
                     /locus_tag="ACZPAQ_04830"
     CDS             complement(2646..>3507)
                     /gene="cgtA"
                     /locus_tag="ACZPAQ_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HIR0903343.1"
                     /translation="FAKPGLPGESREVVLELKLLADVGLVGFPNVGKSTLLSVVSRAQ
                     PKIANYHFTTLFPNLGVVYVEEGVSFVMADIPGIIEGAADGAGLGHDFLRHVDRCRLL
                     IHVVDVSGSEGRDPVEDFEAINAELKQYSPELAGRKMIVAANKVDIMEDPALLDRLRA
                     HVESQGMELFEISAAAHQGTRELVKRAAQELQQLPPVVVYEPTYVERPPEVDTNGSVS
                     IEKFDDTWVVEAPWLQQLIASVNFGDFESRNWFDQKLRQSGLFDKLEEMGIQDGDIVS
                     LYDLEFEYQR"
BASE COUNT          749 a          935 c         1122 g          701 t
ORIGIN      
        1 gcgcgtggag ttcgaccagc cccagcgggc ggtcaccccg ggccaggcag tggtctttta
       61 cgacggcgac ctggtgatcg gcggggccac cattcagaaa ggaagagagt catgagcaaa
      121 aagcgcagcc agagaaagaa ttccaactac aattaccgcc ccggccagaa ggcaggagag
      181 gacccccagg cgctccagga gaacagccgg accaagaaat tcagccccgc ggcccggaat
      241 ctgctgttcg tggacctgat cttcctggcg gtgtgccaaa tgatggagtc cggcggcctg
      301 ctggacgacg tgctctctgc gtggctcacc ctggtgggcc tgatcctgct gctggtggcg
      361 gtgggcatcc aattccagtt ctggcggggc cccaagtcca agatccccaa ggggcccagg
      421 agctgaacgg cctatgaagc gggagtatcg gaggagcgag ccgctcttct cccagtgcgg
      481 gctcaactgc ggcctgtgcc ctatgtacca catccgtgag gagtcccact gtccgggctg
      541 cggcgggccg ggacggccat cctgccccat actccggtgt gccggtgagc acggcggggt
      601 ggagttctgc tccctgtgtc cggattggcc ctgccagcgg tatgtccagg agcaggccga
      661 ctccttcatc ccccaccgga acgcccgcag ggacctggag acggtgaagc gggtgggcct
      721 ggagcactac cgggagacgc tgggcaagaa gatggagggg ctgcgcttcc tgctggagcg
      781 gtgcaatgac ggacggcgga agtcgctgtt ctgcaccgct gtcaacctgc tggagctgga
      841 ggatgtggag gcggtcatag ccaggctgga ggaggagttc ggcccagagt attccccgga
      901 ggagcagaag gaacggggga gccgggcggc tgagctgttc cgggagcggg cgcagaccag
      961 gggggtctcc ctgaagctgg agcggcagcg gaagccctga actggaaaga acgtggaaaa
     1021 ggtggagaaa gaccgatgaa tattcagtgg gaagcgggaa agtacacaga aaactttgat
     1081 tttgtccacc gatacggtga gagcgtgctg gagctggtgg aggcggaagc aggaggccta
     1141 gtggtggatc tggggtgcgg caacggggcc ctgacccaga agctggcgga ccgaggatac
     1201 cgggtgctgg gggtggacga ctccgccgag atggtggccg aggcccgggc ccttcatccc
     1261 ggactgccct tccaaaaggg ggatgcggtc accttccagc tggaggagaa ggcagacgtc
     1321 attttttcta atgctgtgct ccactggatc gacgggagcc ggcagcaggc cctggcccgc
     1381 aacgtggccg cccagctgaa gccgggcggg cagtttatct ttgaattcgg cgggaagggc
     1441 tgcgcggagc gggtccactc gtcgctggag cgacagttcg cgcggagggg gctggcctat
     1501 cccaggacct tctacttccc tactatcggg gagtacgccc ccattttgga gcgggcgggc
     1561 ttccgggtgg aatacgcggt gctgtttgac cggcccacag agcagaagac gggggacggc
     1621 ctggccgact gggtccgcat gttcgtcaaa aaaccctttg aggggttgga gcctgccctg
     1681 ggggatgagc tcctccgggc ggcggtggag gagctgcggc ctgccctgtt ccgcgacggg
     1741 aagtggttcg tggactacgt ccgcatccgc atgaaggcaa ggaagacggc cggatgacca
     1801 agctggccgg gggcaccggt gtgcggtgca cacggagacg tggtgcggag gggagagcca
     1861 aagcagtaga tgaattattc ctttacagaa aaggaaatta ttattaaata tatccaaaaa
     1921 ttttctgaaa tgtaaataaa tatatgttac aatgaaagaa gaaaagaagg gactgtagag
     1981 cctaaaaagg agatggaaaa aatgaagctg aaattgatac ttttggggag cttgtccccg
     2041 ctgattggac tgttgatttt tttccttcaa ttacaggtgc ccttccatgg aataaaatac
     2101 ttagtgatcc tggcgatcat gtctgcggta tttttagcct atttcttttt ctgtgcattc
     2161 tttttttcca gtaaacaaca caaaaagaac agcctatact tttttgtgac tccctttata
     2221 ttgttcttgg tcaattttat aggatggatc caaaaatatg ttgtattggc gctgatcgta
     2281 tcaggggtat ccgcaccatt tcactatgtt ctgccagatt tgatatttcc aggagacaaa
     2341 tatcacttaa taatgagcat atttcctttg gtcatttgtt ggatcgcgtt taaaataggg
     2401 gagagagtgg gtatttactt caaagaaaga atttaatttg acatttaagt cttaccactt
     2461 ctaacatctt aatatctgct cgtttccttc tctaggccca tagatttgct gactgcgcga
     2521 cctccagaac gaaaaacaga agggtatgcc atctgaggca tacccttctg cgttctcgta
     2581 aagatgtgtg tgggaggaac ctataagggg acaaagaaag aggagactga gaaagctgtc
     2641 ggcggttatc tctggtactc gaactccagg tcatacaggg agacgatatc tccgtcctgg
     2701 atgcccatct cctccagttt gtcgaacaga ccggactggc gcagcttctg gtcaaaccag
     2761 ttgcgggact cgaaatcccc aaagttgacg ctggcaatga gctgctggag ccagggggcc
     2821 tccaccaccc aggtgtcatc gaacttctcg atggacacgc tgccgttggt gtccacctcg
     2881 gggggacgct ccacgtaggt gggctcgtag accaccacag ggggcagctg ctggagctcc
     2941 tgggcagcac gcttcaccag ctcccgggtg ccctggtggg cggcggcgga gatctcgaag
     3001 agctccatgc cctgggactc cacatgggcg cgcagccggt ccagcagggc ggggtcctcc
     3061 atgatgtcca ccttgttggc ggccacgatc attttccggc cggccagctc gggggagtac
     3121 tgcttcagct cggcattgat ggcctcgaag tcctccaccg ggtcccggcc ctcggagccg
     3181 gacacatcca ccacgtggat cagcagccgg cagcggtcca catggcgcag aaagtcgtgg
     3241 cccaggccgg caccgtcggc ggcgccctcg atgataccgg ggatatcggc catgacgaag
     3301 gagaccccct cctccacata aaccaccccc agattgggga acagagtggt gaagtggtag
     3361 ttggcgatct tgggctgggc ccggctgacc acgctgagca gggtggactt gcccacgttg
     3421 ggaaagccca ccagccccac gtcggccagc agcttcagct ccagcacaac ctcccggctc
     3481 tcaccgggca ggccgggctt ggcgaag
//
LOCUS       DBMDSJ010000155         3504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6761_length_3504_cov_1.549221, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000155 DBMDSJ010000000
VERSION     DBMDSJ010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6761_length_3504_cov_1.549221"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(167..928)
                     /locus_tag="ACZPAQ_04835"
     CDS             complement(167..928)
                     /locus_tag="ACZPAQ_04835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR0903344.1"
                     /translation="MREEWITSRKNPLLVQIRRLASSRGARRSAGMFLGDGVKLLEEA
                     LKWGAPLETVVYSEGLDGPELPPEVRTVRVPEDVMASISPMESPQGALFLCRMWDTAP
                     PPRLEGGRYLVLDGLQDPGNVGTIWRTADALEADGLLLVHGCADPFGPKVVRATMGAC
                     FRLPVWETGPERLAGLLERSGLALYATALREDTEDIKTADLSRSAVVIGSEGRGVSPE
                     LLAMSQRALKIPMRERCESLNAAAAAAVVLWEMGR"
     gene            complement(925..1557)
                     /locus_tag="ACZPAQ_04840"
     CDS             complement(925..1557)
                     /locus_tag="ACZPAQ_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR0903345.1"
                     /translation="MIGLGRFGTSLACELCALGHEVMAVDLREERVQEVAGSVTHAAA
                     GDARDPEVLRALGARNFDCAVVSAGDDVGTSALIVLNLKELGVPQVVGKARSAVHHRV
                     LEKIGADRVVFPEQEMALRLARNLANGDILNFIELSEEYSIMECRCPRQWQGKSIREL
                     DVRARHRLNIIAIRRGGGMTISPGGEYALQEEDELVVLGRNEDIREVERL"
     gene            complement(1583..3001)
                     /locus_tag="ACZPAQ_04845"
     CDS             complement(1583..3001)
                     /locus_tag="ACZPAQ_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR0903346.1"
                     /translation="MLFCIGTGEPGAPAPAEGGGSVLRLLSNQNFNPTRLIVVSFGAI
                     ILLGTFLLHLPISARDGQFTPWLTCLFTATSATCVTGLVLVDSALHWTPFGQGVILAM
                     IQLGGLGFVSLISLIPLLLRRRIGLSQRLAIASAFNLNGMSGVVRVVRHALMGTFLLE
                     GCGAILLATRFVPLFGWGRGLWYSIFHSVSAFCNAGFDLMGEHTGAFSSLAGFREDPV
                     VLGTIGCLIVLGGLGFFVWEDCLEHRSWRRLSFYSRMVLAVTGALILGGWLFFLGAEW
                     NNLETLGGMTAGEKVGNALFQSVTLRTAGYSVLDQGALREGAAALSVVLMLIGGSSGS
                     TAGGIKTATAGVLLISLWAGLRGRDQVVLRRRTIPQARVLNAMTLTLVVTCLFLAGSI
                     ALTLADGVPYLAAAFEVASAMGTVGLTMGITTGLSPLSQGIIIAMMFLGRVGVLSFSI
                     AFLIRDRGENKIRYPSVDVMIG"
     gene            3276..>3504
                     /locus_tag="ACZPAQ_04850"
     CDS             3276..>3504
                     /locus_tag="ACZPAQ_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014898.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR0903347.1"
                     /translation="MKDHHVPQEHLSAFARRLAEEERSAGTVEKYLRDAGAFLEWLAG
                     APVNREAVTGWKAHLVRSGYRPATINSMLAAL"
BASE COUNT          649 a         1257 c         1053 g          545 t
ORIGIN      
        1 acgctcttgg gatgctcctc ttcggcccca tcccgggcct gctggctgac cgcttccaca
       61 gctatgttcc ggcctatgcc ctgttcgccc tgtgtctggc cgtctcactg gtcatcatcc
      121 agcgggccta ccgaaagctg gacgcaggac accggccccg ccgggattag cggcccatct
      181 cccagagcac caccgcggcg gccgcggcgg cgttcaggga ctcacaccgc tcccgcatag
      241 ggatcttcag ggcccgctgg ctcatagcga gcagttccgg cgagaccccg cgcccctcgc
      301 tcccgatcac cacggcggac cggctcaggt ccgccgtttt gatatcctcc gtatcctccc
      361 gcagggcggt ggcgtacagc gccagtcctg accgctccag caggcccgcc agcctctccg
      421 ggccggtctc ccacaccggg agccggaaac aagcccccat ggtggcccgc accaccttgg
      481 ggccgaaggg gtccgcacat ccgtggacca ggagcaggcc gtccgcctcc agggcatcgg
      541 cggtgcgcca gatagtgcct acgttccccg ggtcctggag accgtccagc accagatagc
      601 gcccgccctc caggcggggc ggcggggcgg tgtcccacat ccggcacagg aacagcgccc
      661 cctgggggga ctccatgggg gagatggagg ccatcacatc ctccggcacc cggaccgtcc
      721 gcacctccgg cgggagctcc ggcccgtcca gcccctccga gtacaccacc gtctccaggg
      781 gcgctcccca cttcagggcc tcctccagca gcttgacgcc gtcccccagg aacatccccg
      841 ctgagcgccg ggcgccgcgg ctggaggcca ggcggcggat ctggaccagg agagggttct
      901 tccggctggt gatccactcc tctctcacag ccgctccacc tctctgatat cctcgttccg
      961 tcccagcacc accagctcgt cctcctcctg aagggcgtac tcacctccgg gggagatggt
     1021 catgccgccc ccccggcgga tagcgatgat gttcaggcgg tgtctggccc gcacgtccag
     1081 ttcccggatg ctcttcccct gccactggcg gggacagcgg cactccatga tggagtactc
     1141 ctccgacagc tcgataaagt tgagaatgtc cccgttggcc aggttccggg ccaggcgcag
     1201 ggccatctcc tgctccggaa agaccacccg gtcggcgccg atcttctcca gcacccggtg
     1261 gtggacggcg ctgcgggcct tgcccaccac ctggggcact cccagctcct tcaggttgag
     1321 gacgatgagg gcgctggtgc ccacgtcgtc ccctgcggac accacagcac agtcaaagtt
     1381 cctggctccc agggcccgga ggacctccgg gtcccgggca tctccggcgg cggcgtgggt
     1441 cacagagccc gccacctcct ggacccgctc ctcccgcaga tccaccgcca tcacttcgtg
     1501 gcccagggca cacagctcac aggccagcga tgtgccgaat ctgcccagtc cgatcaccaa
     1561 aagcttttca tagacatctc cttcagccga tcatcacatc cacactgggg tagcggatct
     1621 tattctctcc ccggtcccgg atcaggaacg caatggagaa ggacagcacg cccacccggc
     1681 ccaggaacat catcgcgatg atgattccct ggctcagcgg ggacaagccg gtggtgatcc
     1741 ccatggtcag ccccacggtc cccatggccg aggccacttc gaaggcggca gccaggtagg
     1801 ggaccccgtc cgccaatgtc agggcgatgg acccggccag gaacaggcag gtcaccacca
     1861 gggtcagggt catggcattg agcacccggg cctgggggat ggtcctccgc cggagcacca
     1921 cctggtctct cccccgcagg ccggcccaca gtgagatcag cagcacgccg gcggtggccg
     1981 tcttgatgcc gccggcggtg gagccggagg agcccccgat cagcatcagc accacggaca
     2041 gggcagccgc cccttcccgc agggccccct ggtccagaac gctgtaccct gctgtgcgca
     2101 gggtcaccga ctgaaagaga gcattgccca ccttctctcc cgccgtcata ccacccaggg
     2161 tctccagatt gttccactct gcccccagaa agaacagcca gccccccagg atcagcgccc
     2221 cggtgacggc aagcaccatt ctcgagtaga aggacagccg ccgccagctc cgatgctcca
     2281 ggcagtcctc ccaaacaaaa aagcccaggc cgcccagcac gatcaggcac ccgatggtgc
     2341 ccagcaccac cgggtcctcc cggaagcccg caaggctgga gaaggccccg gtgtgttccc
     2401 ccatcaggtc gaatccggca ttgcagaaag cggacacgct gtgaaagatg gagtaccaca
     2461 gcccccgccc ccagccgaag agagggacga agcgggtagc cagcaggatc gcaccacagc
     2521 cctccagcag aaaggtgccc atgagggcgt ggcgcaccac ccgcactacc ccggacatcc
     2581 cattcagatt aaatgcggag gcgatggcca gccgctggga caggccgatg cgccgccgca
     2641 gcagcagagg gatcaggctg atcaatgaga caaatcccag cccgcccagc tggatcatgg
     2701 ccaggatcac cccctgaccg aagggagtcc agtggagcgc gctgtccacc aggaccagac
     2761 cggtgacgca ggtggcggag gtggcggtga acaggcaggt cagccagggg gtgaactgcc
     2821 cgtcccgggc ggagataggc aggtggagca gaaaagtccc cagcaggatg atggccccga
     2881 agctcaccac gatcaggcgg gtagggttaa aattctgatt ggacaacagg cgcagcacgc
     2941 tgcctccccc ttctgccggg gccggagccc ccggctcccc tgtcccgatg cagaacagca
     3001 taccggcagt tccccctgtc agtatgctgt gcggcccggg ccctccgtgg attcatacaa
     3061 agttgagtat agcataccct cccaggatat tcaagtcaac gatttttctc ccccgccccg
     3121 cattttcata ccttatatat aaaaaggagt gtccggtgcc ggaggttggg ctgcaaattg
     3181 aacagccttc ctccttttct tcccctaggt ttcggataaa cgccccccgt ttccggtaaa
     3241 atagtaccac actggaagaa aaggggagtt tttgtatgaa agatcaccat gtgccccagg
     3301 aacatctgtc tgcctttgcc cggaggctgg cggaggagga gcggagcgcg gggacagtgg
     3361 agaagtacct gcgggacgca ggggcctttc tggagtggct ggcgggggcg ccggtgaacc
     3421 gggaggcggt cacgggctgg aaggcccact tggtccgctc cggctaccgc cccgccacca
     3481 tcaactccat gctggccgcc ctca
//
LOCUS       DBMDSJ010000156         3492 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6787_length_3492_cov_1.508699, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000156 DBMDSJ010000000
VERSION     DBMDSJ010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6787_length_3492_cov_1.508699"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="ACZPAQ_04855"
     CDS             <1..271
                     /locus_tag="ACZPAQ_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HIR0903348.1"
                     /translation="DVLEYRLRSEYNVELYKEGLPFEYLRWIVKEDEDAPPLKEEDLL
                     LPNDAKLVEDYKGNQLLLFASQWSIQWVQDRNKGLQLAEFGGAKF"
     gene            complement(326..2185)
                     /locus_tag="ACZPAQ_04860"
     CDS             complement(326..2185)
                     /locus_tag="ACZPAQ_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR0903349.1"
                     /translation="MFSKLALRNVRRSFRDYGVYLLTLTFGVCLFYTFNSIDGQGVMV
                     YLAQSQNPMAQLIQTILDIFSVFVAVVLACLILYANTFLIRRRKRELGTYLLLGLSQR
                     QVSGLLVLETLVIGLISLAAGLLLGLAASQGLSALTLSMFSIRVDAFALAFSPRAAVR
                     TVGYFGLIFLLVMVFSGIQVSRARLIDLMQGERKNEELRERPLGLAVVQFVLGAVCLA
                     AAYAILLTFGMAIAVAVLPICAAMLFLGTLGTLLIFKSLSGFVVKLVQSRPGLYFRGL
                     NLFTLRQWLSRVHSTYLSMTVICILLLLAVGITASSIGLNSTLEAAADEEAPFDLTVQ
                     NQSADSAGLVDFPAVLAEGGFDLDRLGEQLDVLFYYNDPAVTGVEEVSAAIRLSDYNA
                     LLAHGGRGSCHEEVPMVPDDPERRAVTGGLSLSYAVVPDELAGQLEVRRQVWFVDYAG
                     GDEERTEAELIQLIRPLDLDYALHFSSRLDAYQSIMGSKILALFLGLYLGFTFLLAAA
                     AVLALQQLSQAADNQKRYAMLRRLGADDGLIARSASQQVALAFLMPLGLALIHSMVGM
                     KAANDIIATAGEVDSVGSSLATAAVLILVYGGYFLATALACRRMARQSWTKLE"
     gene            complement(2175..2942)
                     /locus_tag="ACZPAQ_04865"
     CDS             complement(2175..2942)
                     /locus_tag="ACZPAQ_04865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903350.1"
                     /translation="MEEILKVTDLKKVYGKRGAATTALNGVSFSLRGGEYVGIMGASG
                     SGKTTLLNCVSTIDRPTSGSILVDGKELSDLRGKELARFRRERLGFIFQDCNLLDTLT
                     AFENIALALSIVRTPAGEIREQVEENARLLGIQDCLDKYPYQMSGGQQQRCAAARAIV
                     TRPALVLADEPTGALDSRSARMLLERLEELNQIRQTTILMVTHDAFTASCCHRVIFLR
                     DGQIFLELSRGGMDRRAFFAQIIRVVSQLGGEMEDVL"
     gene            complement(3155..>3492)
                     /locus_tag="ACZPAQ_04870"
     CDS             complement(3155..>3492)
                     /locus_tag="ACZPAQ_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012936.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR0903351.1"
                     /translation="MKAMNGQRKQVLRDAVEGLLPEELLRRPKSPYPKTHDPLYEQLV
                     RARLLTILDDRDAPIHSLVDEKALRAGLLSDAGDYGKPWFGQLMAGPQMLAYLIQINE
                     WMSRYHLTV"
BASE COUNT          703 a         1124 c         1090 g          575 t
ORIGIN      
        1 cgacgtgctg gagtaccgcc tgcgcagcga gtacaacgtg gagctgtaca aggagggcct
       61 gcccttcgag tacctgcgct ggatcgtgaa ggaggacgag gacgcccccc cgctgaagga
      121 ggaggacctg ctgctcccca acgacgccaa gctggtggag gactacaagg gcaatcagct
      181 gctgctgttc gcctcccagt ggtccatcca gtgggtccag gaccggaaca agggccttca
      241 gctggcagag ttcggcggcg caaaatttta aaaacatcaa atcagcggct ccgtggaagc
      301 tctcttccgc ggagccgctg ttctttcatt caagcttggt ccagctctgc cgggccatcc
      361 gacggcaggc cagggctgtg gccaggaagt agcctccgta gaccaggatc agcacagcgg
      421 ctgtggccag gctgctcccc accgagtcca cctcgcccgc cgtggcaatg atgtcgttgg
      481 ccgccttcat ccccaccatg gagtggatca gggccagccc caggggcatc aggaaggcca
      541 gagccacctg ctgggaggcc gagcgggcga tgaggccgtc gtccgccccc agccggcgga
      601 gcatggcgta ccgcttctgg ttgtccgccg cctggctcag ctgctggagg gccagcaccg
      661 cggccgccgc cagcaggaag gtgaagccca gatacagccc cagaaacagg gccaggatct
      721 tggagcccat gatgctctga taggcgtcca ggcggctgga gaagtggagg gcgtagtcca
      781 ggtccagggg gcggatcagc tggatcaact cggcctccgt ccgctcctcg tcccctccgg
      841 cgtagtccac aaaccacacc tgccgccgca cctccagctg tccagccagc tcgtctggga
      901 ccactgcgta gctcagggac agcccgccgg tgaccgcccg gcgctctggg tcgtcaggca
      961 ccatgggcac ctcctcatga cagctccccc gcccgccgtg ggccagcagg gcgttatagt
     1021 cggagagccg gatagccgcc gacacctcct ccaccccggt gaccgctggg tcattgtagt
     1081 aaaacagtac gtccagctgc tctcccagac ggtccaggtc aaagccgccc tccgccagca
     1141 cggccgggaa gtccacaagg cccgcgctgt cggcggactg attctgtacc gtcagatcaa
     1201 agggagcctc ttcatcggcc gccgcctcca aggtggagtt gagtccgatg gagctggcgg
     1261 tgatgccgac ggccagcagc agcaggatgc agatgaccgt catggacagg taggtggagt
     1321 ggaccctgct cagccactgg cgcagggtga ataggttcag cccccggaag tagagcccgg
     1381 gccggctctg gaccagcttc accacaaagc cggacaggga cttaaagatc agcagagtgc
     1441 ccagggtgcc caggaacagc atggccgcac agatgggcag gaccgccaca gcgatggcca
     1501 tgccaaaggt cagcaggatg gcataggcgg ccgccaggca gaccgccccc agcacgaact
     1561 ggaccaccgc cagccccagg ggcctctccc gcagctcctc atttttccgc tccccctgca
     1621 ttaggtcgat aagccgggcc cgggacacct ggatgccgct gaacaccatc acgagcagaa
     1681 agatcaggcc gaaatagccc actgtgcgca ccgctgcccg aggggagaag gccagggcga
     1741 aggcgtctac ccggatggag aacatggaca gagtgagggc agacaggccc tgggaggccg
     1801 ccaggcccaa cagcagcccc gccgccagag agatcaggcc gatgaccagc gtctccagca
     1861 ccagcagtcc tgacacctgc cgctggctca ggcccagcag cagataggtc cccagctccc
     1921 gcttgcggcg gcggatgagg aacgtgttgg catacaggat caaacaggcc agcaccaccg
     1981 ccacaaagac ggagaaaata tccagaatgg tctggatcag ctgggccatg gggttttggc
     2041 tctgagccag atagaccatt accccctgcc cgtcgatgga attaaaggta taaaacagac
     2101 acacgccaaa ggtgagggtg agcagataca ccccgtagtc ccgaaaggag cggcggacgt
     2161 tccgcagggc cagcttagag aacatcttcc atctctcctc ccagttggga cactacccgg
     2221 atgatctggg cgaagaacgc ccgccggtcc atcccgcccc ggctcagttc caggaagatc
     2281 tgcccgtccc gcaggaagat cacccggtgg cagcagctgg cggtgaaggc gtcgtgggtc
     2341 accatcagaa tggtggtctg ccggatctgg ttcagctcct ccagccgctc cagcagcatc
     2401 cgggcggatc tggagtccag cgcgccggtg ggctcgtcgg ccagcaccag ggcgggtcgg
     2461 gtgacgatgg cccgggccgc agcgcagcgc tgctgctgcc cgccggacat ctggtagggg
     2521 tatttgtcca gacagtcctg gatgcccagc agccgggcgt tctcctccac ctgttcccgg
     2581 atctctcccg ccggcgtcct gacgatggac agggccaggg cgatattctc aaaggccgtc
     2641 agggtgtcca gcaggttgca gtcctgaaag ataaagccca gccgttcccg gcggaagcgg
     2701 gccagttcct ttcccctcag gtcggacaat tccttcccat ctaccaagat ggagccggag
     2761 gtgggccggt cgatggtgga gacacagttg agcagagtgg tcttgcccga gccggaggcc
     2821 cccatgatgc ccacatattc tccgcctctc agggaaaagc tgaccccgtt cagggccgtc
     2881 gtagcggccc cccgctttcc atagaccttt tttaggtctg tgaccttcaa gatttcttcc
     2941 atattcatgc gctccttgcc gcaggcggct ccgccgcccc aaaatggttt attgaaactg
     3001 tatgaatgat accatacagt tgcttcaaat gctctaaagg agctcttagg gttttcttaa
     3061 gaaatcataa agccagggac aaaaaagcgc gccccggatg gggcgcgcca gaaagcagga
     3121 ggaacaaaca ggagaaacgc actgtcaccg caggtcagac agtcaagtgg tatcgggaca
     3181 tccattcgtt gatctgaatg aggtaggcca gcatctgggg cccggccatc agctgcccga
     3241 accagggctt tccatagtcc cctgcgtcgg acaacagccc tgcgcgcagg gctttctcgt
     3301 ccaccaggct gtggatgggg gcgtcccggt cgtccaggat ggtgagcagc ctggcccgga
     3361 ccagctgctc atacaggggg tcgtgggtct tggggtaggg gctcttgggc cggcggagca
     3421 gctcctccgg gagcaggcct tcgaccgcgt ccctcagcac ctgcttgcgc tgcccgttca
     3481 tagccttcat tg
//
LOCUS       DBMDSJ010000157         3490 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6794_length_3490_cov_1.521393, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000157 DBMDSJ010000000
VERSION     DBMDSJ010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_3490_cov_1.521393"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="ACZPAQ_04875"
     CDS             complement(<1..125)
                     /locus_tag="ACZPAQ_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR0903352.1"
                     /translation="MAKVKEEKTNVMRVLDQKKIPYTPHAYPHGDGEAPDGVTVAR"
     gene            complement(125..568)
                     /locus_tag="ACZPAQ_04880"
     CDS             complement(125..568)
                     /locus_tag="ACZPAQ_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903353.1"
                     /translation="MEHTYLLRPGRWRAEGTYYDETGRSFPLAGETELVQEGAERHLD
                     GWMEVSGPAPVRFTNRYVIRETDRPFTLTWQSFNPVLGVLEGTFELAGESILSRYVSR
                     DRVYSGCEVLTLQADGSYHNAGVSMKNGRRMSAWTAVLSEERGRT"
     gene            complement(574..741)
                     /locus_tag="ACZPAQ_04885"
     CDS             complement(574..741)
                     /locus_tag="ACZPAQ_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903354.1"
                     /translation="MKETVTKCARSPVWQKGYHDHIVRGEAEYLRIWNYIDTNPAKWR
                     EDCYYCQNEGV"
     gene            complement(1088..3061)
                     /gene="uvrB"
                     /locus_tag="ACZPAQ_04890"
     CDS             complement(1088..3061)
                     /gene="uvrB"
                     /locus_tag="ACZPAQ_04890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR0903355.1"
                     /translation="MPRFEVVSEYTPSGDQPEAIEALASGLEMGLDEQTLLGVTGSGK
                     TFTMAKIIEKVQRPTLVLAHNKTLAAQLCAEFREFFPHNAVEYFVSYYDYYQPEAYIP
                     HTDTFIEKDSAINEEIDRLRLSATASLMERRDVIVVSSVSCIYGLGEPEDFAKMMVSL
                     RVGATLERDELLKKLVAIRYERNDIAFDRNMFRVRGDTVEIWPAYWKDTALRVEFFGD
                     EIDRISEINVVTGTPIRRLNNIPIWPATHYVTPKEKMDAAIQEIYKELEERVAFFEGE
                     GKLVEAQRLKQRTMYDVEMMQELGYCSGIENYSRVIEGRPVGSPPHTLLDYFPKDFVL
                     FIDESHVTLPQVRAMYNGDRARKTTLVDYGFRLPCAFDNRPLKFEEFEKRLNQVVYVS
                     ATPGEYERTRSGQIVEQVIRPTGLLDPRIEVRPVEGQIDDLIGEINQRTQRSERVLVT
                     TLTKRMAEDLTAYLQNAGIKVRYMHHDIDTIERMEIIRDLRLGTFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTIGRAARNADGMVVMYADTITPSMRRAIDET
                     ERRREKQDAFNKAHGIVPRTVIKSVRELLEISATAEDVASNQRQGRVKAMTRQEKSAE
                     IARLEKAMKEAAKMLEFELAATLRDQIIELRGK"
     gene            complement(3166..>3490)
                     /locus_tag="ACZPAQ_04895"
     CDS             complement(3166..>3490)
                     /locus_tag="ACZPAQ_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR0903356.1"
                     /translation="GARNEMIYLLALDGKNKPLGVRKVAEGSICAANINLRRIMEEAA
                     ALRAPQIYLAHNHISNLAFPSPVDISTTQTMRTALAGVGIELLDHLVFVDGDMVSMRD
                     SGMMK"
BASE COUNT          591 a         1114 c         1052 g          733 t
ORIGIN      
        1 cgggccaccg tcaccccgtc gggggcctcg ccgtccccgt ggggataggc gtggggggtg
       61 tagggaatct tcttctggtc caggacccgc atgacgttgg ttttttcctc ttttacctta
      121 gccattatgt ccttcccctc tcctcgctca gcacggcggt ccaggcggac atgcgccgtc
      181 cgttcttcat agacacgccc gcgttgtgat agctgccgtc cgcctggagg gtcagcacct
      241 cacagccgga gtacacccgg tcccgtgaga catagcggga caggatactc tctcccgcca
      301 gctcgaaagt accctccagc acgcccagca ccgggttgaa ggactgccag gtcagggtaa
      361 aggggcggtc cgtctcccgg atgacatagc ggttcgtgaa tcggaccgga gcgggcccgg
      421 acacctccat ccagccgtcc agatgccgct ccgccccctc ctggaccagc tctgtctccc
      481 cggccagggg aaaggagcgg cccgtctcgt cgtagtaggt cccttccgcc cgccagcggc
      541 cgggacggag cagataggtg tgttccacag taactacact ccctcattct ggcagtagta
      601 acagtcctcc cgccacttgg caggattcgt gtcgatgtag ttccaaatgc ggagatactc
      661 cgcctcaccg cggacgatgt ggtcgtgata gcccttttgc cagacggggg acctggcaca
      721 tttggtgacc gtctctttca ttgctttgat gatccgggga accgctccga cgggagtagg
      781 ggcgcacagt gtgcgcccgg ttccctcgat acgcaacata aggtggatat tattgggcat
      841 gactacatat ttttccacaa aaacatacgg ataatgattt ggaatacttt gaattgcccg
      901 ttccgtaatt tccccgattt ctgtgagagg cggaatttcc gatgcgggcg cacactgtgc
      961 gcccctacgc aaaattggct gccgcccagc tgtgcaaatg gtgatgaaat aacaccccgg
     1021 ctggctgtaa tcgtatccgg tcaaacggtt cggcttccgt ttcgggagca gctccataga
     1081 attcttctca cttcccccgc agttcgatga tctggtctct caaagttgcc gccagctcga
     1141 actccagcat cttggccgcc tctttcatgg ctttctccaa gcgggcgatc tcggcggact
     1201 tctcctgcct ggtcatggcc ttgaccctgc cctggcgctg gttggaggcc acgtcctccg
     1261 ccgtggcgga gatctccagc agctcccgca ccgacttgat gaccgttctg gggacgatgc
     1321 cgtgggcctt gttgaaggcg tcctgcttct cccggcggcg ctccgtctcg tcgatggccc
     1381 ggcgcatgga gggggtgatg gtgtccgcgt acatcaccac catgccgtcg gcgttccggg
     1441 cggcccggcc gatggtctgg atgagggaag tctcggaccg gaggaagccc tccttgtccg
     1501 cgtccaggat ggccaccagg gagacctcgg gcaggtccag gccctcccgg agcagattga
     1561 tgcccaccag cacgtcgaag gtgcccagcc gcaggtcccg gatgatctcc atccgctcga
     1621 tggtgtcgat gtcgtggtgc atatagcgca ccttgatgcc cgcgttctgg aggtaggcgg
     1681 tcaggtcctc cgccatccgc ttggtcaggg ttgtgaccag cacccgctcg ctccgctggg
     1741 tgcgctggtt gatctccccg atcaggtcgt cgatctggcc ctccaccggc ctgacctcga
     1801 tcctggggtc cagcagcccg gtgggccgga tcacctgctc cacgatctgg cccgagcggg
     1861 tgcgctcata ctcccccggg gtggcggaga catagaccac ctggttgagc cgcttttcaa
     1921 attcctcaaa tttcagcggc cggttgtcaa aggcgcaggg gagccggaag ccgtaatcca
     1981 ccagggtggt ctttcgggcc cggtccccgt tatacatggc ccgcacctgg ggcagggtga
     2041 cgtggctctc gtcgatgaag aggacgaagt ctttggggaa gtagtccagc agggtgtggg
     2101 ggggcgagcc cacgggccgc ccctcaatga cccgggaata gttctcgatg ccggagcagt
     2161 agccaagctc ctgcatcatc tctacgtcgt acatggtgcg ctgcttcagc cgctgggcct
     2221 ccaccagctt gccctctccc tcgaagaagg ccacccgctc ctccagttcc ttgtagatct
     2281 cctggatagc ggcgtccatc ttctccttgg gggtgacata gtgggtggcc ggccagatgg
     2341 ggatgttgtt cagccggcgg atgggggtgc cggtgaccac gttgatctcg ctgatgcggt
     2401 cgatctcgtc cccgaaaaac tccacccgca gggcggtatc cttccaatag gcgggccaga
     2461 tctccaccgt gtccccccgg acccggaaca tgttgcggtc aaaggcgata tcgttgcgtt
     2521 catagcggat ggccaccagc tttttcagca gctcgtcccg ctccagggtg gcccccaccc
     2581 gcagggagac catcatcttg gcgaagtcct ccggctcgcc cagaccgtag atacaggaga
     2641 cggaggagac cacgatcacg tccctccgct ccatcaggga ggcggtggcg gacagcctca
     2701 gccggtcgat ctcctcgttg atggcggaat ccttttcgat gaaggtgtct gtgtggggga
     2761 tataggcctc cggctggtag tagtcgtagt aggagacgaa gtactccacc gcgttgtggg
     2821 gaaagaactc ccggaactcg gcacacagct gggcggccag ggtcttgttg tgggccagca
     2881 caagggtggg ccgctggacc ttttcgatga tcttggccat ggtgaaggtc ttgcccgagc
     2941 cggtgacgcc cagcagcgtc tgctcgtcca gccccatctc cagtccgctg gccagggcct
     3001 cgatggcctc cggctgatcc ccgctggggg tgtattctga aaccacttca aatctgggca
     3061 tagccgtccc tcctgttcct cccgggccgc tcttcgtccg ccctattata ataaaacaaa
     3121 tgttctaagt caaggccctg tccacagagg gacagggccc aggcatcact tcatcatccc
     3181 gctgtcccgc atggacacca tatccccgtc cacaaagacc aggtgatcca gcagctcgat
     3241 gcccaccccc gccagggccg tccgcatggt ctgagttgtg gagatgtcca ccggggaggg
     3301 gaaggccagg ttgctgatgt ggttgtgggc cagatagatc tggggcgccc ggagggcggc
     3361 tgcctcctcc atgatccggc gcagattgat gttggcggcg cagatgctgc cctccgccac
     3421 cttccgcacc cccagaggct tgttcttccc atccagcgca agcaggtaga tcatctcgtt
     3481 cctggccccg
//
LOCUS       DBMDSJ010000158         3452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6891_length_3452_cov_1.530588, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000158 DBMDSJ010000000
VERSION     DBMDSJ010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6891_length_3452_cov_1.530588"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(188..1546)
                     /gene="glmM"
                     /locus_tag="ACZPAQ_04900"
     CDS             complement(188..1546)
                     /gene="glmM"
                     /locus_tag="ACZPAQ_04900"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR0903357.1"
                     /translation="MGKLFGTDGIRGVVNAGLDADLAYKVGLAAAEVLAKGKQPGEKP
                     LVTIGKDTRISGDLLKGSLIAGLCTAGADVLDLGTLPTPGVAWVTVDEGADAGIVISA
                     SHNPFEHNGIKIFNGQGFKLSDELEEKIEEIVLFGHNDVPRKTHGEIGKVSYVAPKAS
                     EDYIDHLESTIDSTLGGLRILVDCANGAASATAARLFDRFSKLRTDVINADPDGVNIN
                     EKCGSTHMDALAAMVRAGGYDIGIAFDGDADRCLAVDERGELIDGDQIMAACGLDMKR
                     KGKLPGNAVVATVMSNLGLHLFAKEHGMDLECTSVGDRNVLERMLEKGYAIGGEQSGH
                     MIFLEHATTGDGQLTALQVLALLKESGKRASEVFGICQRYPQVLINIPVADNDRKKAV
                     MASQALADAVAAEEEKLAGEGRVLVRPSGTEALMRVMVEARDQDTAQAAAQRLADQIA
                     AL"
     gene            complement(1553..1930)
                     /locus_tag="ACZPAQ_04905"
     CDS             complement(1553..1930)
                     /locus_tag="ACZPAQ_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HIR0903358.1"
                     /translation="MGFWLFCTASSLLIPLVMLFFGRRFLTKPPKSINSLYGYRTARS
                     MKNQQTWDFAHRVCGKLWSRAGAVMLPLSLLAMLPALGRGTEETGMWCIPVTGVQLAV
                     LVGSMFPVERALKENFDQFGRKR"
     gene            complement(1951..3024)
                     /gene="ruvB"
                     /locus_tag="ACZPAQ_04910"
     CDS             complement(1951..3024)
                     /gene="ruvB"
                     /locus_tag="ACZPAQ_04910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR0903359.1"
                     /translation="MAIDFSNTEFEAEGLEEPLVTTSLTREDEGEYSLRPKTLREYIG
                     QEKAKGNLEVFIQAAKMRHEPLDHVLLHGPPGLGKTTLSGIIANEMGVNVRITSGPAI
                     EKAGDLAALLTNLNENDILFVDEIHRLNRAVEEVLYPAMEDYAIDIIIGKGPSANSIR
                     LDLPRFTLIGATTRAGQLSAPLRDRFGVTLRLELYTPEELALIVTRSAGILEVPIEPE
                     GAMEIARRSRGTPRIANRMLRRVRDFAQVRAGGVITRNVADQALTALEIDHLGLDAID
                     HRMLRSIMENYRGGPVGLETLAATINEEAVTLEDVYEPYLMQLGFLTRTPRGRCVTPK
                     AYQHLGLPMPGGAGGGGLDQLSF"
     assembly_gap    3082..3091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3214..>3452)
                     /locus_tag="ACZPAQ_04915"
     CDS             complement(3214..>3452)
                     /locus_tag="ACZPAQ_04915"
                     /inference="COORDINATES: protein motif:HMM:NF040311.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HIR0903360.1"
                     /translation="ETEGARFIVRVFERFSLTDRDLVSLSLLLADLGDGRIRLTGITA
                     GCGRLEKLWTYGEEAFLKKLTKLADGFDAAHPQQ"
BASE COUNT          602 a         1153 c         1028 g          659 t
ORIGIN      
        1 gacggcagag cgattcatcc gagtaagact gtgtttggga ctgcaccaaa tccatatact
       61 atggctccta tacataataa acaaaaccga cggatttgtc aagaaaaatt ttgaatcgat
      121 ttgtgaaagt ctcacagagc aggaaaactg ccccttgtca cgaacttcca caccttccag
      181 ttttctgtca cagggcggcg atctggtccg ccagccgctg cgccgccgcc tgggccgtat
      241 cctgatccct ggcctccacc atgacccgca tcagggcctc agtgcccgag gggcgcacca
      301 gcacccggcc ctcgccggcc agcttctcct cctccgcagc cacggcatcg gccagggcct
      361 ggctggccat aacggccttt ttcctgtcgt tgtcggcgac cgggatgttg atgagcacct
      421 ggggataccg ctggcagatc ccgaacacct cagaggcccg tttcccgctc tccttcagca
      481 gagcgagcac ctggagggcg gtgagctgcc cgtctccggt ggtggcgtgc tccaggaaga
      541 tcatgtgtcc cgactgctct cctccaatgg catagccctt ctccagcata cgctccagca
      601 cgttgcggtc gcccacggag gtgcactcca ggtccatccc gtgctctttg gcgaacagat
      661 gcagccccag attggacatg acggtggcca ccaccgcgtt tcctggcagc ttccccttcc
      721 gcttcatgtc cagaccgcat gccgccatga tctggtcgcc gtcgatcagc tccccccgct
      781 catccactgc caggcagcgg tctgcgtcgc cgtcaaaggc gataccgatg tcatagcccc
      841 cagcccttac catggcggcc aaggcgtcca tgtgggtgga accgcacttt tcgttgatgt
      901 tgaccccgtc cgggtcggcg ttgatgacgt cggtgcgcag cttggagaag cggtcgaaca
      961 ggcgggcggc ggtggcggag gcggcaccat tggcacagtc caccaggatg cgcaggccgc
     1021 ccagagtgga gtcgatggtg gactccaggt ggtcgatgta gtcctctgac gccttggggg
     1081 ccacataact cactttgccg atctccccgt gggtctttcg gggcacgtcg ttgtggccga
     1141 agaggacgat ctcctcgatc ttctcctcca gctcgtcgga cagcttgaag ccctggccgt
     1201 tgaagatctt gatgccattg tgctcgaagg ggttgtgact ggcggagatg acgatgcccg
     1261 catccgcccc ctcgtccacc gtcacccagg ccacgccggg ggtgggcagg gtgcccaggt
     1321 ccagcacgtc ggcccccgcg gtgcacagcc ccgcgatgag ggagcccttc agcaggtcgc
     1381 cggagatgcg ggtatccttg ccgatggtga ccaggggctt ctcccccggc tgttttccct
     1441 tggccagcac ctccgccgcc gccaggccca ccttataagc caagtccgca tccagaccgg
     1501 cgttgaccac gccccggatg ccgtcagttc cgaatagttt acccatatat gattacctct
     1561 ttcttccgaa ttgatcaaag ttttccttca atgcccgctc cacagggaac atactgccca
     1621 caagcactgc cagctgcacc cctgtcaccg gaatacacca catcccggtc tcctctgtgc
     1681 cccgccccaa ggcaggcagc atagccagca ggctcagagg aagcatgaca gcccccgccc
     1741 gagaccagag cttcccgcac actcggtggg caaagtccca ggtctgctga tttttcatgg
     1801 atcgtgcggt gcggtaaccg tagagggagt tgatgctctt gggcggcttt gtcaaaaaac
     1861 gccggccaaa aaacagcatc accagcggga tcagcaggga cgaggcggtg cagaaaagcc
     1921 aaaagcccat ggtcctctcc cctttccgat ttagaagctc agctggtcca gtccgccgcc
     1981 gcccgcgccc ccgggcatcg gcagccccag gtgctgatag gccttgggag tgacgcagcg
     2041 gccccggggg gtcctggtga ggaagcccag ctgcatcagg tagggctcat acacgtcctc
     2101 cagggtgacc gcctcctcgt tgatggtggc ggccagggtc tccagcccca ccgggccgcc
     2161 ccggtagttt tccataatgc tgcgaagcat ccggtgatcg atggcgtcca gccccagatg
     2221 gtcgatctcc agggcggtaa gggcctggtc tgccacgttc cgggtgatga cgcccccagc
     2281 ccgcacctgg gcgaagtccc gcacccggcg gagcatccgg ttggcgatgc ggggcgtccc
     2341 ccgggagcgg cgtgcgatct ccatggctcc ctccggctcg atgggcacct ccaggatgcc
     2401 ggcggaacgg gtgacgatca gggccagctc ctccggagtg taaagctcca gccgcagcgt
     2461 cacaccgaat cggtcccgca ggggggcgga cagctgtccg gcccgggtag tggccccgat
     2521 gagggtgaac ctgggcaggt ccaggcggat ggagttagct gagggcccct tgccgatgat
     2581 gatgtcgatg gcgtagtcct ccatggcagg gtagagcacc tcctccaccg cccggttcag
     2641 gcggtggatc tcgtctacaa agaggatgtc gttctcgttc aggttggtaa gcagggcggc
     2701 caggtccccc gccttctcga tggctgggcc ggaggtgatg cgcacgttca cccccatctc
     2761 attggcgatg atgccgctga gggtggtctt gcccagtcca ggggggccgt ggagcagcac
     2821 atggtccagg ggctcatggc gcatcttggc cgcctgaatg aacacctcca ggttgccctt
     2881 ggccttctcc tggccgatgt actcccgcag ggtcttgggc cgcagggagt actctccctc
     2941 gtcctcccgg gtgagggagg tggtcaccag gggctcctcc agcccctccg cttcaaattc
     3001 tgtgttggaa aaatcaatgg ccataggtcc acttcctgtt cattggtccg atcccggccg
     3061 cacgcgcccc gccggtcagg gnnnnnnnnn ntattcccga taggccccct ccgggagctc
     3121 catgccgaag ccgtcgaaaa agatcttagg cggctcggcg ctgatgacct ctagcaaggt
     3181 gccagcggcc gccagcagag ggcgccctct cccctactgt tgtgggtggg cggcatcgaa
     3241 cccgtctgcc agcttcgtca gcttcttcaa aaaggcctcc tcgccatagg tccatagctt
     3301 ctccagcctg ccgcagccgg cggtgatccc tgtgagccga atcctgccgt cccccaggtc
     3361 ggccagcagc aggctcagcg agaccagatc ccggtctgtc aggctgaacc gctcaaacac
     3421 ccggacaata aagcgcgccc cctctgtctc at
//
LOCUS       DBMDSJ010000159         3434 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6929_length_3434_cov_1.537654, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000159 DBMDSJ010000000
VERSION     DBMDSJ010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6929_length_3434_cov_1.537654"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="ACZPAQ_04920"
     CDS             complement(<1..1409)
                     /locus_tag="ACZPAQ_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR0903361.1"
                     /translation="MLELLAPAGSMEAVTAAVQNGADAVYLGYGDFNARRNAKNFTGD
                     EAAAAVSYCHLRGVKVFLTLNTLLTDRELAGAAQEAAQASAMGVDAVLVQDLGVLRML
                     RQTVPDLPVHASTQMTVHSLDGVKQCADLGMSRVVLSRELSRDQIEYICARSPVEIEV
                     FAHGALCMCYSGQCFFSSVIGGRSGNRGLCAQPCRLKYGWGNKADGSPLSLKDMSLAG
                     HLRELRKMGVACVKIEGRMKRPEYVAVVTGIYARAIREGREPTAEELEQLRSAFSRDG
                     FTDGYFLGQTGPEMFGTRQDEAEPRELFTQARATYESGENRKEPVRMYAMLRRGEPAQ
                     VAVQDAQEHLASVTGPVPEEAVNVPLTREKVEGQLSRTGGTPFSCEKVTVHVDEGLSL
                     PLSALNALRRQALEELGRQRTALPERRCEPFRPGVRYENRKQPPVYTLSVRAAEQVSP
                     ELLRLAPALLYLPAGEGAA"
     gene            complement(1499..1813)
                     /locus_tag="ACZPAQ_04925"
     CDS             complement(1499..1813)
                     /locus_tag="ACZPAQ_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR0903362.1"
                     /translation="MKNKITVSIAGQEYTMVAAEGEDYVRRVAAHVDSQMREVLSQGR
                     LSQADGAVLAAMNIADQYFKEQEAAENLRRQIKEYLEEATKLKMELSEAKREIFKLQN
                     HK"
     gene            1997..2947
                     /locus_tag="ACZPAQ_04930"
     CDS             1997..2947
                     /locus_tag="ACZPAQ_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR0903363.1"
                     /translation="MTGPLMPGSILSMPAAAAERLIRLDSGDAALLYLYLLGHGNAAG
                     LNWPRERLQGALDLLSAQGMAGAEAAPPPPAPPVQEALPPEYTTQDVTAALQEAGSTF
                     SALADEVERRLGKRLSANDLKILLTLYDHLALPGEVILMLVTWCIEETERKYGPGRRP
                     FLSQVRKEGFAWSRQGIDTVEAAEAHLKKLAQLHTREREVLRLLDIPARPLVERERTY
                     IAAWQDMGFDNEALRLAYEKTVMKKQSMDWGYMNGILRRWHEKGLHTVAAIQAGDGLR
                     RNRGGAPAQGRTPQPGEERRVREDMERMRRLMEQMKREEE"
     gene            2951..>3434
                     /locus_tag="ACZPAQ_04935"
     CDS             2951..>3434
                     /locus_tag="ACZPAQ_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR0903364.1"
                     /translation="MSYDPAVLRRATARLEGERRAREERQQALRRAACQRQPRLSEID
                     RQLRGTMTELFAASLRRGEGGAPTVEEVRRKNLALQQERAELLVRMDLAEDALDDKPA
                     CPLCGDTGWRGTEMCRCLRALCAQEQIRELSKLLDLGEQSFDTFRMDYYSTSFWPEWG
                     T"
BASE COUNT          549 a         1140 c         1194 g          551 t
ORIGIN      
        1 tgggccgccc cctccccggc ggggaggtag aggagggcgg gggcgaggcg gagcagctcc
       61 gggctgacct gttcggcggc gcgcacagag agggtgtaga cagggggctg cttccggttc
      121 tcatagcgca ccccggggcg gaagggctcg cagcggcgct ccggcagtgc ggtgcgctgc
      181 cggcccagct cctccagagc ctgccggcgc agggcgttga gagccgacag ggggagggag
      241 agcccttcat ccacgtggac ggtgaccttc tcacaggaga agggagtccc tccggtgcgg
      301 gagagctgtc cctccacctt ctcacgggtc agggggacgt tcaccgcctc ctccggcacc
      361 gggccggtga cggaggccag atgctcctga gcgtcctgga cggccacctg ggcgggctcc
      421 ccccggcgga gcatggcgta catccgcacc ggctccttcc ggttctcccc gctctcatag
      481 gtggcccggg cctgggtgaa cagctcccgg ggctccgcct cgtcctgacg ggtgccgaac
      541 atctccggcc ccgtctgccc cagaaagtag ccgtccgtga agccgtcccg ggagaaggcg
      601 gagcgcagct gctccagctc ctcggccgtg ggctcccgcc cctcccggat ggcccgggca
      661 tagatgccag tgaccacggc cacatactcg gggcgcttca tccgcccctc gatcttaaca
      721 caggccaccc ccatcttcct cagttcccgg aggtggcctg ccagggacat gtccttcagg
      781 gacagggggc tgccgtctgc cttgttcccc cagccgtact tcagccggca gggctgggca
      841 cacagccccc ggttgccgct gcgtcccccg atgacggagg agaagaagca ctggccggag
      901 tagcacatgc acagggcccc gtgggcgaac acctcgatct ccaccggaga gcgggcgcag
      961 atgtactcga tctggtcccg ggacagctcc cgggagagga ccacccggct catccccagg
     1021 tcggcgcact gcttcacccc gtccaggctg tggactgtca tctgggtgga ggcatggacc
     1081 ggcaggtcgg gcaccgtctg ccgaagcatc cgcagcaccc ccagatcctg gaccagtacc
     1141 gcgtccaccc ccatggcgct ggcctgggcg gcctcctgag cggcgccggc cagctcccgg
     1201 tcggtgagca gggtgttcag ggtgaggaac accttgacgc cccgcagatg gcaataagaa
     1261 accgccgccg cagcttcatc cccggtgaag ttcttcgcgt tgcggcgggc gttgaaatcc
     1321 ccatatccca gatagacggc gtctgccccg ttctgcacgg cggcggtgac cgcctccatg
     1381 gacccggcag gggcgagaag ctccagcata aggcacctcc cgtaaagatc cgccctcagg
     1441 cggcgcgtcc gcagggaaga ggggcccccg tccccggcgg ggaacccctc tcctgcgctt
     1501 atttgtggtt ctggagcttg aagatctccc gcttggcctc ggagagctcc attttcagct
     1561 tggtggcctc ctccagatac tccttgatct ggcggcgcag gttttcggcg gcctcctgct
     1621 ccttgaaata ctgatccgcg atgttcatgg cggccagaac ggcgccgtcc gcctgggaga
     1681 gacggccctg ggagaggacc tcccgcatct ggctgtccac atgggcggcc acccggcgga
     1741 catagtcctc gccctcggcg gccaccatgg tgtattcctg tcccgcgatg gagacggtga
     1801 tcttgttttt catcgtgaag cccctcccgg tctctgctgt ttcaagatgg caatcattat
     1861 accacagaac aggccgcttg aaaaacaaat ttaccatgaa ttttttattt ttttgccctt
     1921 gggattttgg aggtttacgc cggggcgatt ttagagtaaa atggtcgcag acttttgtgg
     1981 aagggagggg catgggatga ccggacccct gatgccgggc agcatcctgt ccatgccggc
     2041 ggcggcggcc gagcggctga tccggctgga cagcggagac gccgccctgc tgtatctcta
     2101 cctgctgggc cacgggaacg ccgccggcct gaactggccc cgggagcggc tccagggggc
     2161 gctggacctg ctctccgccc agggaatggc gggggcggag gccgctccgc cgcccccggc
     2221 accccctgtt caggaggcgc tgccccccga atacaccacc caggacgtca ctgccgccct
     2281 ccaggaggcg ggctccacct tctccgccct ggcggacgag gtggagcgcc gcctgggcaa
     2341 gcggctgtct gccaatgacc tgaagatcct gctcaccctg tatgaccact tggccctgcc
     2401 cggcgaggtg atcctgatgc tggtcacttg gtgcatcgag gagacggagc ggaagtacgg
     2461 gccggggcgg cgccccttcc tctcccaggt gcgcaaggag ggctttgcct ggtccaggca
     2521 gggcatcgac accgtggagg cggcggaggc ccacctgaaa aagctggctc agctgcacac
     2581 ccgggaacgg gaggtgctgc ggctgctgga catcccggcc cgccccctgg tggagcggga
     2641 gaggacctat atcgccgcct ggcaggatat gggctttgac aacgaggccc tgcgcctggc
     2701 ctatgagaag acggtgatga aaaagcagtc tatggactgg ggctatatga acggcatcct
     2761 gcgccgctgg cacgagaagg ggctgcacac cgtggcggcc atccaggccg gggacggcct
     2821 gcgccggaac cggggcgggg cgccggccca ggggcggact cctcagcccg gagaggagcg
     2881 ccgggtgcgg gaggacatgg agcggatgcg ccgcctgatg gagcagatga agcgggagga
     2941 ggaataagag atgtcatatg accctgcggt cctgcgccgg gccaccgccc ggctggaggg
     3001 ggagcgccgc gcccgggagg agcggcagca ggccctgcgg cgggccgctt gtcagcgcca
     3061 gccccggctg tcggagatcg accggcagct gcggggcacc atgaccgagc tgtttgccgc
     3121 ctccctgcgc cggggggagg ggggcgctcc cacggtggag gaggtgcggc ggaagaacct
     3181 ggccctccag caggagcggg cggagctgct ggtccgcatg gatctggcgg aggacgccct
     3241 ggatgacaag cccgcctgcc ccctgtgcgg ggacacgggc tggagaggca ctgagatgtg
     3301 ccgctgcctg cgggcgctgt gcgcccagga gcagatccgg gagctgtcca agctgctgga
     3361 cctgggggag cagtccttcg acaccttccg gatggactat tacagcacca gcttctggcc
     3421 cgaatggggc acct
//
LOCUS       DBMDSJ010000160         3426 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6954_length_3426_cov_1.616241, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000160 DBMDSJ010000000
VERSION     DBMDSJ010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6954_length_3426_cov_1.616241"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="ACZPAQ_04940"
     CDS             complement(<1..506)
                     /locus_tag="ACZPAQ_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0903365.1"
                     /translation="MDYTVYQLLWLFLFYSFLGWLAETVLAALKRRRLLNRGFLSAPF
                     SPTYGLGALLFAIFLPELREDPFFLFLLGMILATFLEFVTGVFLERIFHQKWWDYSGQ
                     RFQFEGHICLKYSVIWGLSALLAIFVLNPIFLTLVGLIPRTAGEIALLVVYILLGVDF
                     VGSWAAVL"
     gene            complement(586..1740)
                     /locus_tag="ACZPAQ_04945"
     CDS             complement(586..1740)
                     /locus_tag="ACZPAQ_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-tRNA(Ala) deacylase AlaX"
                     /protein_id="HIR0903366.1"
                     /translation="MEKLYQQDPFLTRFTAQVVSCAADKGGWSVVLDRTAFYPEGGGQ
                     PWDTGTLNGIPVTQVQEQDGAVVHTCAARLEPGTQVEGAVDWARRFDLMQQHSGEHIV
                     SGLIHSTYGYDNVGFHLGSDLVTIDFNGPLEEEDLRDIELRANQYIWEDRPVSVTYPT
                     TKELAALDYRSKKAISGQVRIVSFPGADTCACCGTHVASAGQVGLVKLLSVQKFREGV
                     RVELLCGGRALSYLDRVLGQNRRISNLLSAKPFETAGGVERLLGENQDLKTRLARMEE
                     ARFAQLADGLAGAGDVILFEEGLSPDGLRRLCDAVLQVCGGRCACFSGGDGEGYKYAI
                     GLRGGDLRSFVRELNQALDGRGGGRPDFVQGSVRAGRTAIEEFLSAEKQR"
     gene            complement(1930..3003)
                     /locus_tag="ACZPAQ_04950"
     CDS             complement(1930..3003)
                     /locus_tag="ACZPAQ_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR0903367.1"
                     /translation="MKRTFSALVISGALLCTLAVPGMAAEAEGQPGDSAAASAPQAQT
                     LPASVLYFGQVTQVIRDEAGTVTRLVLSSERYGEYIMNISSDTVWIDSGNRTASDPAD
                     LKEGESVYVFHSPISTRSLPPQSAAYAVVRNIPQDISCAQYHVVEEITEGEDGGLIVT
                     TDNGGLLIYLDGDTQTVRYAGGSAALEDLKAGDRIMAWYQAVALSYPGQTHADGLMLL
                     PSEKEEPSSVLPENGTALDLVLEGDMVLPVTGLVENGTAMVPVAAVAQALGYQVIYTP
                     GEDGEGDRIAVESDQFSVQMTVGEDQIIGVTKIKGAVGMTAPADYGMAPYIAAPGTTW
                     APARLFEMLGRTVTLEGDALSIR"
     gene            3187..>3426
                     /locus_tag="ACZPAQ_04955"
     CDS             3187..>3426
                     /locus_tag="ACZPAQ_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903368.1"
                     /translation="MTDESFTSAVRTYGDTVYRVAYHALKNRADAEDVMQTTLLRLLE
                     HRQPFESEEHMKHWLLRVAVNESRKLLRSFWRRHAV"
BASE COUNT          730 a         1073 c          998 g          625 t
ORIGIN      
        1 tggagcacag cggcccagga gccaacaaag tccactccca gcagaatata caccaccagc
       61 agtgcgatct ctcccgccgt cctggggatc aggcccacca gtgtaaggaa gatgggattc
      121 agaacaaaga tggccagcag agcggacaag ccccagataa cagagtattt caggcagata
      181 tgtccttcaa actgaaaccg ctggccggaa tagtcccacc atttctgatg aaagatgcgc
      241 tccaggaata caccggtaac aaactccaga aatgtggcga ggatcatgcc caaaaggaag
      301 aggaaaaatg ggtcttcccg cagttcaggc agaaaaatgg caaaaagcag cgcccccagg
      361 ccataggtgg gagagaaggg ggcgctgagg aagccgcggt tgaggaggcg gcggcgtttc
      421 agcgcggcta gaacggtttc ggccagccag cccaggaagg aatagaacag gaagagccag
      481 agcagctgat agacagtata atccatagga tctccttatc aagatggtcg aaaggcagta
      541 tgccgttttt cccgacctct gtgccgggag gcgtacacca tttgtttatc tctgtttttc
      601 ggcggacagg aactcttcta tggcagtcct cccggcccgg accgaaccct gaacaaagtc
      661 cggcctgcct ccgccccggc cgtccagggc ctgattcagt tccctcacaa aggagcgcag
      721 atctccgccc cgcaggccga tggcgtattt atacccctcc ccatcgcccc cggaaaagca
      781 ggcacagcgg cctccgcaga cttggaggac tgcgtcacac aggcggcgca gtccgtcggg
      841 ggacagcccc tcctcgaaaa ggatcacatc ccccgcccca gccagaccat cggccagctg
      901 ggcaaaccgt gcctcctcca tacgggcgag gcgggtcttc aggtcctgat tctcccccag
      961 caggcgctcc accccgccgg ccgtctcaaa gggcttggcg gaaagcagat tggagatccg
     1021 ccggttctgg cccagcaccc gatccagata gctcagggcc cggccgccgc agagcagctc
     1081 cacccgcacg ccctcccgga acttctgtac ggagagaagc ttgaccagcc ccacctgtcc
     1141 ggcggatgcc acatgggtcc cgcagcaggc gcaggtgtcc gccccaggga aggagacaat
     1201 gcggacctgc ccagagatcg cctttttgct ccgatagtcc agcgcagcca gctccttcgt
     1261 cgtggggtag gtgaccgata cggggcggtc ctcccagatg tactggttgg cccgcagctc
     1321 gatgtcccgc aggtcttcct cctccagcgg cccgttgaaa tcgatggtca ccaggtcgct
     1381 gcccagatgg aagcccacgt tgtcatagcc gtaggtactg tggatcaggc cggagacgat
     1441 atgctccccg gagtgctgct gcatcaggtc gaagcgccgc gcccagtcta cagccccctc
     1501 cacctgagtc cccggctcca ggcgggcggc gcaggtgtgg accaccgctc catcctgctc
     1561 ctggacctgg gtgactggga tgccgttcag cgtaccggtg tcccagggct gccctccccc
     1621 ttcaggatag aaggccgtgc ggtccagcac cacagaccac ccacccttgt cagcagcaca
     1681 ggacaccacc tgggcagtga agcgtgtcag gaaggggtcc tgctggtaca gcttttccat
     1741 atgaaaaact cctctcggat cgaaatggca gaaacagcat accacattcc gtaggaaaat
     1801 gcaaaaaaag agagacggct gatctgggcg gtatcgcctt ctaaatccac gacagccggc
     1861 actgtccaaa agacagtgcc ggctgagcgt tctcgatatt ccagagctcc tttttcaggg
     1921 ggccccggct caccggatgg acagggcatc gccctccagg gtgactgtgc ggcccaacat
     1981 ctcaaagagc cgggcaggag cccaggtggt gccgggggcg gcaatgtagg gggccatccc
     2041 atagtccgca ggggcggtca tccccactgc ccccttgatc ttcgtcacgc caatgatctg
     2101 gtcctctccc accgtcatct ggacagagaa ctgatcgctc tccacggcga tcctgtcacc
     2161 ctctccatct tcgccggggg tgtagatcac ctgatagcca agcgcctggg ccacagcggc
     2221 cacggggacc atggcggtgc cgttttcaac gaggccggta acgggcagga ccatatctcc
     2281 ctccagcacc aggtccagcg ccgtgccgtt ctccgggaga acggaggacg gttcctcctt
     2341 ctcactgggc aggagcatca ggccgtcagc gtgggtctgt ccgggatagg acagggccac
     2401 cgcctgatac caggccatga tccggtctcc tgccttcagg tcctccagtg cggcagagcc
     2461 gcccgcataa cggacagtct gggtatcacc gtccagatag atcagcagtc cgccgttgtc
     2521 tgtcgtgacg atcaggccgc cgtcctcccc ctctgtgatc tcctccacca catggtactg
     2581 ggcgcaggag atatcctgag ggatattccg gaccaccgca taggctgcgg actggggcgg
     2641 aagggagcgg gtggaaatgg ggctgtggaa cacatagacg ctctccccct ccttcaggtc
     2701 cgcgggatct gatgcagtgc ggttcccgct gtcgatccat actgtatcag aagaaatatt
     2761 catgatatac tcaccgtagc gctcagagga gaggaccagg cgggtgactg tgcccgcttc
     2821 atcccgaatg acctgggtca cttgtccaaa gtacagcacg ctggccggga gagtctgggc
     2881 ctgaggtgca gaggcggcgg cggaatctcc aggctgacct tcggcttccg ctgccatacc
     2941 ggggaccgca agggtgcaga gcagggcgcc gcttatcacg agagcagaga aggttcgttt
     3001 catatcataa cactcctttt tctttcaaac gctcggaaat ttttccttgc actgagatag
     3061 acggtcaaag ggtaaaaaag ttccccctct ggagaaattt ttttgttccc tatgagatat
     3121 ttttccgtgt ccggggatat tcctggtgaa aagcttttca agatcccgca aaggaggtga
     3181 gctcccctga cagacgagtc ctttacctcg gctgttcgga cctatggaga cacagtttac
     3241 cgcgtggcct atcacgccct aaaaaaccgg gccgacgccg aggatgtgat gcagaccacc
     3301 ctgctccggc tgctggagca ccggcagccc tttgagagcg aggagcacat gaagcactgg
     3361 ctgctccggg tggccgtcaa cgagagccgg aagctgctgc gctccttctg gcgcaggcac
     3421 gccgtc
//
LOCUS       DBMDSJ010000161         3419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_6978_length_3419_cov_1.717601, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000161 DBMDSJ010000000
VERSION     DBMDSJ010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6978_length_3419_cov_1.717601"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..523
                     /locus_tag="ACZPAQ_04960"
     CDS             2..523
                     /locus_tag="ACZPAQ_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR0903369.1"
                     /translation="MASKLLGHLLMEQGYDVKVSEVHGMSQRGGSVVTYVRYGDRVNS
                     PVIDKGEADYIVSFELLEAARWLSYLKPDGQIVTSTQQIDPMPVITGAAQYPENLVEK
                     MKAAGAKVDALDCLALAEEAGSSKAVNIVLMGRLSHYFDLPDEAWQKSLEVCVPSKFL
                     ELNKKAFQLGKNA"
     gene            539..1102
                     /locus_tag="ACZPAQ_04965"
     CDS             539..1102
                     /locus_tag="ACZPAQ_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024812.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR0903370.1"
                     /translation="MAEERVPLEIRRAEPEDAAELLSIYAPYVEESWVTFECVPPTVE
                     EFRRRMEEFSRHYPYLVCCRAGEILGYAYAHRHMEREAYQWNAELTVYVRRGREGLGI
                     GTALYGALIPKLREMGLVNLYGVLGLPNPGSEALHRRFGFTRQGLYPRMGFKLGEWHD
                     VAQYGLRLTPPDRPPEPLFRTEGGTQG"
     gene            1099..1302
                     /locus_tag="ACZPAQ_04970"
     CDS             1099..1302
                     /locus_tag="ACZPAQ_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903371.1"
                     /translation="MIWKYLLRIWGWVCLLAAALHGALTLYAAAVPYNIYQPGSTGLA
                     EWLGMGLISHGLAHVMARLDKLG"
     gene            1348..1560
                     /locus_tag="ACZPAQ_04975"
     CDS             1348..1560
                     /locus_tag="ACZPAQ_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903372.1"
                     /translation="MKFVIVIEWLLGLWGAFALYVALVCGIAFFDASTPENLAMFLLS
                     GCAALFSFALIAVLELLRKKQQTSPK"
     gene            1633..2934
                     /locus_tag="ACZPAQ_04980"
     CDS             1633..2934
                     /locus_tag="ACZPAQ_04980"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR0903373.1"
                     /translation="MERYYQPEIETASREQIKAWQDERLVKQVQHVWDNVPYYRKKME
                     EKGVTPADIKSSDDLYKLPFLTKSDLRDAYPYGLVGMPLKDCVRIQSTSGTTGKRVVA
                     FYTQEDIDLWEDCCARAIMAAGGTNEDVCQVSYGYGLFTGGPGLNGGSHKVGCLTIPT
                     SSGNTERQIMFIKDLNATILCCTPSYAAYIGETMKEMGMSPEEIPLKAGIFGAEAWSE
                     EMRQDIQNTLGIKAYDIYGLTELSGPGVSFECSAQRGMHINEDHFIAEIIDPDTGEVL
                     PEGSEGELVFTSITKKAFPLLRYRTRDICTLTREKCPCGRTHVKMSKPRGRTDDMLII
                     RGVNVFPSQIETVLLNYGYPANYQIIVDRVHSTDTLDVQVEMTPEMFTDKVGEVDRRQ
                     KELVDGLRSMLGLSAKVTLVAPKSIVRSEGKAVRVIDKRKI"
     gene            2959..3390
                     /locus_tag="ACZPAQ_04985"
     CDS             2959..3390
                     /locus_tag="ACZPAQ_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HIR0903374.1"
                     /translation="MSVKQLSVFLENRPGALYGMTGILSQNKIDMRALSVAETRDFGI
                     LRIIVDDPYKTATVLKDAGYVYNITPVLGVAIPDVPGGLNKVLQVLTDARINVEYMYA
                     FLGGKTEAAYMIFRVEDNNAAAEALASRGIKTLEEADIQRM"
BASE COUNT          695 a          987 c         1102 g          635 t
ORIGIN      
        1 gctggccagc aagctgctgg gccatctgct gatggagcag ggctatgacg taaaggtgtc
       61 cgaggtccac ggcatgtccc agcggggcgg cagcgtggtc acctatgtgc gctacggcga
      121 tcgggtaaat tcccctgtta tcgacaaggg ggaggctgac tacatcgtct ccttcgagct
      181 gctggaggcc gcccgctggc tcagctatct gaagcccgac ggccagatcg tcacctccac
      241 ccagcagatc gatcccatgc ccgttatcac cggcgcggcc cagtacccgg aaaacctggt
      301 ggagaagatg aaagcggccg gcgccaaggt ggacgccctg gactgcttgg ctctggccga
      361 ggaggcaggc tcctccaagg cggtgaacat cgtgctgatg ggccggctct cccactactt
      421 cgatctgccc gacgaggcgt ggcagaagtc cctggaggtc tgtgtgcctt ccaagttcct
      481 ggagctgaac aagaaggcat tccagctggg taagaacgcc tgaaaggagg gcggagagat
      541 ggcggaggag cgcgtccctt tggagatccg cagggcagag ccggaggacg cggcggagct
      601 gctgtccatc tatgccccat atgtggagga gagctgggtg acctttgagt gcgtcccgcc
      661 cacggtggag gagttccggc gccgtatgga ggaattttcc cggcactacc cctatctggt
      721 gtgctgccgg gcgggggaga tcctgggcta tgcctacgcc caccgccaca tggagcggga
      781 ggcctaccag tggaacgcgg agctcactgt ctatgtgcgc cggggtcggg agggcttggg
      841 gatcggcacc gccctctacg gggcgctcat ccccaagctg cgggaaatgg ggctggtcaa
      901 cctgtacggg gtgctgggac tgcccaaccc gggcagtgag gccctgcacc gccgcttcgg
      961 ctttacccgg caggggctgt accctcggat gggattcaag ctgggggagt ggcacgacgt
     1021 ggcccagtac ggcctgcgcc tcaccccccc ggaccggccg ccggaacccc tttttcggac
     1081 agaagggggg acgcagggat gatctggaag tatctgctgc ggatctgggg ttgggtgtgc
     1141 ctgctggcag ccgccctgca tggggctttg accctgtatg cggccgctgt gccgtacaat
     1201 atttatcagc cgggaagcac cggactggcg gagtggctgg gcatggggct gatctcccac
     1261 ggcctggccc atgtgatggc ccgcctggac aagctgggat gatcgcaggg ggtccataaa
     1321 aaaatcagga cagagggggg gacatggatg aaatttgtca ttgtcataga gtggcttttg
     1381 gggctttggg gagcatttgc cctctatgtg gctctggtgt gcggaattgc tttttttgat
     1441 gcgagcacgc cggaaaatct ggccatgttc ctgttgagcg ggtgtgccgc cctgttttcc
     1501 tttgctctga tcgcggtgct ggaactgctt cggaaaaagc agcagacatc gcctaaataa
     1561 aagccgagaa tttctttgac tcttttttgc atgaaagaga agaaatatga aaacgagaga
     1621 gggatgattc cattggaacg ctattatcag ccggagatcg agactgcgtc ccgggaacag
     1681 atcaaggcct ggcaggacga gcgcctggta aagcaggtcc agcatgtgtg ggacaacgtg
     1741 ccctactacc gcaaaaagat ggaggagaag ggggtcacac cggcggatat caagtccagc
     1801 gacgacctgt acaagctgcc cttcctcacc aagtctgacc tgcgggacgc ttacccctac
     1861 gggctggtgg ggatgcccct gaaggactgc gtccgcatcc agtccacctc tggcaccact
     1921 ggcaagcggg tggtggcctt ctacactcag gaggacatcg atctctggga ggactgctgt
     1981 gcccgagcta tcatggctgc ggggggcacc aatgaggatg tgtgccaggt gtcctacggc
     2041 tacggactgt tcaccggcgg ccccggcctc aacggaggca gccacaaggt gggctgcctc
     2101 accatcccca cctcctcggg caacaccgag cgtcagatca tgttcatcaa agacttgaac
     2161 gccaccatcc tgtgctgcac cccctcctat gccgcctaca tcggcgagac catgaaggag
     2221 atgggaatga gtccggagga gatccccctg aaggcgggca tattcggtgc ggaagcctgg
     2281 tccgaggaga tgcgccagga cattcagaat accctgggca tcaaggcata tgatatctac
     2341 gggctcaccg agctgtccgg ccccggtgtc tccttcgagt gctccgccca gagagggatg
     2401 cacatcaacg aggaccactt catcgctgag atcatcgacc cggacaccgg cgaggtgctg
     2461 cccgagggca gcgagggcga gctggtattc acctccatca caaagaaggc gtttcctctg
     2521 ctgcgctacc gcacccggga catctgcacc ctcacccggg aaaagtgtcc ctgcggccgc
     2581 acccatgtga agatgtccaa gcccaggggc cgcaccgacg acatgctcat catccggggg
     2641 gtcaacgtat tcccctccca gatcgagacg gtgctcctca attacggtta tcccgccaac
     2701 taccagatca tcgtggatcg ggtccactcc accgacaccc tggacgtgca ggtggagatg
     2761 accccggaga tgtttaccga caaggtgggc gaggtggacc gccgccagaa ggagttggtg
     2821 gacggcctgc gctccatgct ggggctgtcc gccaaggtca ccctggtggc tcccaagtcc
     2881 atcgtccgca gcgagggcaa ggcggtccgt gtcatcgaca agcgcaagat ctgactgatt
     2941 tgacaggagg gaagagttat gagcgtcaag cagctttccg tgttcctgga gaataggccc
     3001 ggtgccctgt atgggatgac cggtatcctc tcccagaaca agatcgacat gcgggccctc
     3061 tcggtggcgg agacccggga tttcggcatc ctccgcatca tcgtggacga cccgtacaag
     3121 acggccactg tcctgaagga cgcgggctat gtgtacaaca tcacccccgt gctgggggtg
     3181 gcgatccctg acgtgccggg cggattgaac aaggtactcc aggtgctcac cgatgccagg
     3241 atcaacgtgg agtacatgta tgcattcctg gggggcaaga ccgaagccgc ctatatgatc
     3301 ttccgggtag aggacaacaa tgccgcggcg gaggccctgg cctcccgggg catcaagacc
     3361 ctggaggaag cggatatcca gcgaatgtaa agcgaaacag ctttcaagaa aaaggacag
//
LOCUS       DBMDSJ010000162         3411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7004_length_3411_cov_1.430514, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000162 DBMDSJ010000000
VERSION     DBMDSJ010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7004_length_3411_cov_1.430514"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="ACZPAQ_04990"
     CDS             complement(<1..591)
                     /locus_tag="ACZPAQ_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR0903375.1"
                     /translation="MYAIIDIGSNTIRLNVYQVEEDGAIHSIFQSKETAGLAGYVQED
                     GSMSREGLDRAADALLRFRSILDALHIENRAVFATASLRNITNSEEAVFQLQERTGLM
                     IRLLSGREEAILDYVGATSGMEEREQGMVLDIGGGSTEWVCFSQGRIRHAASIPLGSL
                     NLYSRCVETLLPSPKEQAKIRKQVKKALDSAPLPDFP"
     gene            831..1856
                     /locus_tag="ACZPAQ_04995"
                     /pseudo
     CDS             831..1856
                     /locus_tag="ACZPAQ_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
     gene            1996..2460
                     /locus_tag="ACZPAQ_05000"
     CDS             1996..2460
                     /locus_tag="ACZPAQ_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903376.1"
                     /translation="MKKSVRNFIKIYWEVLLVVAAGIAGVILLKVKGEDWPAALNFPT
                     EAVAAGYWILILGMTALEGILIFRFLVKGLKYVIYTVLPLWLYLMFASPGGLLFPSLI
                     WTYSLVILIARLVMLIRCRLKKQDRQFSHLSSMICIDVISTINILREFVLFG"
     gene            complement(2508..>3411)
                     /locus_tag="ACZPAQ_05005"
     CDS             complement(2508..>3411)
                     /locus_tag="ACZPAQ_05005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0903377.1"
                     /translation="YTADQFYITVGAAASLCCVFNGLTCPGDEFVVFSPYFPEYKVFI
                     EGAGAKMVLIPPEIEGFQIDFDAFEAAISEHTKGVVVNSPNNPSGVVYSRETLERLAA
                     ILTEKSRAYGHPIYLISDEPYREIVFSGFQVPWIPQLYPDTIVCYSFSKSLSLPGERL
                     GYVLVPASVTDSREVYAAVAGAGRSLGYVNAPSLFQQVTSLCCDLTSDLAVYERNAKL
                     LVPALREMGYHCVEPGGAFYLFPRSLEPDDMAFSERAKQFDLLLVPGSGFGAPGHVRI
                     AYCVQTDMIQRALPKFKALADSYR"
BASE COUNT          719 a          879 c         1153 g          660 t
ORIGIN      
        1 gggaaagtcg ggcagggggg cgctgtccag cgcctttttc acctgcttgc ggatcttggc
       61 ctgttctttg ggggagggga gcagggtctc cacacagcgg ctatacaggt tcagggagcc
      121 cagggggatg gaggcggcgt ggcgtatcct gccctgagag aagcagaccc actcggtgga
      181 gccgccgccg atgtccagca ccatgccctg ctcccgctcc tccatgccgg aggtggctcc
      241 cacatagtcc aggatggcct cctcccggcc ggagaggagg cggatcatca gtccggtgcg
      301 ctcctggagc tggaagaccg cctcctcgct gttggtaatg ttgcgcagag aggcggtggc
      361 gaagaccgcc cggttctcaa tgtggagggc atccaggatg gaccggaagc ggagcagggc
      421 atctgcggcc cggtcgaggc cctcccggct catggagccg tcctcctgaa catagccggc
      481 caggccggcc gtctccttgc tttgaaaaat gctgtggata gctccgtcct cctccacctg
      541 atagacattc aggcggatgg tattggagcc gatgtcgata atagcgtaca tgcaagctct
      601 cctctgtggc tgcggtccgc tcggaagcgg gaccggatca tctgagtcgt atgatatcaa
      661 atgcaggggg ctgggtccag aaataggatg cagaattttg gttaaatgta tgtaaaatca
      721 ctggctgaac agacaagacg atgatttttg acggcagagt atagagatcc ccggggaaat
      781 gtggtaaact tccttcatct ggagatctta cagagaggaa ggaactgagc atgctgtctt
      841 ttgtcaacga ctactctgag ggggcccacg agcaggtgct ccggcggctg gcggagacca
      901 atcgggagca gcatcccgga tatgggaccg atccatactg cgcccgggca aaggagaaga
      961 ttcggcgggc ctgcggctgc ccggaggggg acgtcttttt cctggtgggc gggacccaga
     1021 ccaatcaggc ggtgatctcc gccctgctgg ccccctggga gggggtgatc acccccgcca
     1081 cgggacacat ctgtacccat gaggcgggag ccatcgaggc aaccgggcat aaggtactgg
     1141 agctgcccca gcgggagggg aagctgtcag cggcggagct gcggacttat ctggagggct
     1201 tttacgggga cgagaaccac gaacacatgg tctttccggg catggtgtac ctgtcctgac
     1261 ccaccgagct gggcaccctc tatacccggg cggagctggg ggagatccac caggtgtgcc
     1321 ggcaatacaa gataccgctg tacctggacg gcgcccggat gggctatggg ctggccagcc
     1381 gggggagcgg catgaccctg gcggacctgg cggccagctg cgacgtgttc tacatcgggg
     1441 ggaccaaggt gggggccctg ttcggagagg cggtggtctt cccccggcac aacgcccccg
     1501 ggcgctttat gaccgccgtg aagcagcggg gcgccctgct ggccaagggc tggctgctgg
     1561 gggtgcagtt cgacgccctg ttcaccgatg gactctacca gaagctgggc aaaaatgcca
     1621 tcgagacagc ggagcggctg agagagggat tccaggaaaa ggggttctcc ttctatttgg
     1681 acacccccac caaccagata ttcctggtgg tggaccgggc ccagcgggag gacctggccc
     1741 ggaaggccgc attcagcttc tgggagcagc tggacgagga gcggacggtg atccgcttcg
     1801 tcaccagctg ggccacccgg atggaggacg tggaggagtt gatccgtcgg ctgtgaagcg
     1861 ggctgtttga atcatttggg aggaaaaaag agattgttct tgttggaaat agacaaaaag
     1921 attgacaaat aaaagaaaaa gatacatatt attctgaaag aaggcggggg cgatggtcag
     1981 ataacggaga gatgtatgaa aaaatcagtg agaaatttca taaaaatcta ttgggaagtt
     2041 ctgcttgtag tggctgctgg aattgcaggt gtgatacttt taaaagtaaa aggtgaagat
     2101 tggcctgctg cgttgaattt tcccacagag gcagtagcgg ctggctattg gatcttgata
     2161 ctgggaatga cggcattaga aggaatcctg attttccgtt ttctggtgaa agggctgaaa
     2221 tatgtaatat atactgttct gccgctatgg ctctacctta tgtttgcctc gccaggagga
     2281 ctgcttttcc caagcctgat ttggacatat agcttggtga tcctgatcgc ccggcttgtg
     2341 atgctgataa ggtgcaggct aaaaaaacag gaccggcagt tttctcatct gtcatctatg
     2401 atatgtattg atgtgatcag caccataaac atccttcgag aatttgtatt gtttggatag
     2461 cggtaaaaag catcggcccc atccatcacg gatggggccg atgcttttta tcggtaggaa
     2521 tcggccaggg ccttgaattt gggaagggcc cgctggatca tgtcggtctg gacgcagtag
     2581 gcgatgcgca cgtggccggg ggcgccgaag ccggagccgg ggaccagcag cagatcgaac
     2641 tgcttggccc gctcgctgaa ggccatatcg tcgggctcca gggagcgggg gaagaggtag
     2701 aaggcgccgc cgggctccac acagtggtag cccatctccc gcagggcggg gaccagcagc
     2761 ttggcgttgc gctcatagac cgccaggtcg gaggtgaggt cgcagcacag ggaggtgacc
     2821 tgctggaaca ggctgggggc gttgacgtag cccagagacc gtccggcgcc ggccacggcg
     2881 gcgtagacct cccggctgtc ggtgacggag gcggggacca gcacgtagcc caggcgctca
     2941 ccagggaggg agagggactt gctgaaggag tagcacacga tggtgtctgg gtagagctgg
     3001 gggatccagg gcacctggaa gccggagaac acgatctccc ggtagggctc gtcggagatc
     3061 aggtagatgg ggtggccgta ggcccggctc ttttccgtca ggatggcggc cagccgctcc
     3121 agcgtctccc gggagtagac cacgccggag gggttgttgg gggagttgac caccacgccc
     3181 ttggtgtgct cgctgatggc ggcctcaaag gcgtcaaagt cgatctggaa tccctcgatc
     3241 tccgggggaa tgagcaccat cttggcccca gcgccctcga taaagacctt atactccggg
     3301 aagtaggggg aaaagacgac gaactcgtcc ccagggcagg tcaggccgtt gaacacacag
     3361 cacagggagg cggcggcccc cacggtaatg tagaactggt cggcggtata g
//
LOCUS       DBMDSJ010000163         3406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7016_length_3406_cov_1.419062, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000163 DBMDSJ010000000
VERSION     DBMDSJ010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7016_length_3406_cov_1.419062"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="ACZPAQ_05010"
     CDS             <1..528
                     /locus_tag="ACZPAQ_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HIR0903378.1"
                     /translation="EEGVDEAEKHDHSHYRDWERAGFVELCEGDIVDYSRVEEVIREA
                     AAMFRLEILGMDAAMAWTLSQRLMTAAPGSKALELVTIPQTMLGMSPATKKLELLIRE
                     HKMLHEHNTCARYCFGNVRCAVDGNENMKPMKNQSRGRIDITVAWIIAMAAAMLKEQQ
                     KPDLAEVMRTRNYHL"
     gene            553..732
                     /locus_tag="ACZPAQ_05015"
     CDS             553..732
                     /locus_tag="ACZPAQ_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903379.1"
                     /translation="MKNLVNCLAKHLGELVLVGGAAVVAVGAGMIYLPAGLITGGGLA
                     IAGAVLSLWGAGEEK"
     gene            729..2105
                     /locus_tag="ACZPAQ_05020"
     CDS             729..2105
                     /locus_tag="ACZPAQ_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR0903380.1"
                     /translation="MSLRKGLARAGKSSAVRKGLAGVGRLLTLDNPEGWLSGEELVGL
                     SRDRAMKISTVNRCVELLSTSMAVLPVYIMEERTKNRRPDHHLGRVLWERPNEAMTPF
                     DFRRLLMCNQLLRGNAYAWIYRDAVSGLPMELIPLPPDYVSMHLDLSGKVWYLFTHPV
                     TGEVTRIRCEDMLHYKAYSEDGLEGISVLRRASLTLDTARAAQLYENSIWRNGGQPSG
                     ILTTETDLGGEYEVDLEDGTTVKRDPKEQLRQSWEAIHSGPGNAFKVAVLDLGLKYQP
                     ISMNNTDAQFVESSEIRVADVCRFFGVPLHLAYAGKQSYQSNEQNGIEYVTYTLMGYD
                     TQWGQEDTYKLLLPGERAKGLRIKREMKVFLRGDTAAQAAWLKAMREIGAYCSDEIRA
                     LDDLPAIPGGKEYYSSLNYVPLELWRILSIIRALGKAADVPEEPPLGEDPEGAEDPPA
                     KEGEKANE"
     gene            2098..2790
                     /locus_tag="ACZPAQ_05025"
     CDS             2098..2790
                     /locus_tag="ACZPAQ_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903381.1"
                     /translation="MNEILKAAHVEKQAVSEEELALINAQTLRALTADEVFTFRLAAC
                     DDQVDRDYERFTLAALEGLAPLFVGRPVLMDHKWSAGTQTARIYAAGVEEAENVHRLV
                     LRCYMPRTEQTASTITAIESGILRECSVGCAVERALCSICGADQVQTCCQHWPGREYD
                     GRLCVMELDGAKDAYEVSLVAVPAQPGAGIVKSKRYGGRESPDEPGDDEVFQLAMARQ
                     EQEEMRYGGNEL"
     gene            2787..>3406
                     /locus_tag="ACZPAQ_05030"
     CDS             2787..>3406
                     /locus_tag="ACZPAQ_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR0903382.1"
                     /translation="MTYQEYLELKAKRAGKLKEGEALLAKKDFDGHKALMGEVAKMNQ
                     ELDAAEAQLAEEGRFAEDDEGMKLRHKAFQAQKADKAKGAAIDEIRRSNEYAAAFVKA
                     LRNGVKVNKVWGMEGYEPLAKALTETGGSPEGADGGFLVPQDFDDMIHEYEKEYVDLS
                     QFFAVENVRSLSGWRAVEKGKRKALPKITEMGTIGKDDQPKFTKVT"
BASE COUNT          708 a          965 c         1217 g          516 t
ORIGIN      
        1 gaggagggcg tggacgaggc ggagaagcac gaccacagcc attaccggga ctgggagcgg
       61 gccggcttcg tggagctgtg cgagggcgac atcgtggact acagccgggt ggaggaggtc
      121 atccgtgagg cggccgccat gttccgcctt gagatcctgg gcatggacgc cgccatggcg
      181 tggaccctgt cccagcggct gatgacggcg gcaccgggga gcaaggcact ggagctggtg
      241 actatcccgc agaccatgct ggggatgtcc cccgccacca agaagctgga gcttctgatc
      301 cgggaacaca aaatgctcca tgaacacaac acctgcgccc ggtactgctt cggcaacgtg
      361 cggtgcgcgg tggacggcaa tgagaacatg aagcccatga agaaccagag ccgcggccgc
      421 atcgacatca cggtggcctg gatcatcgcc atggcggcgg ccatgctgaa ggagcagcag
      481 aagccggacc tggccgaggt aatgaggacg agaaactatc acctgtaggc cgatggccgg
      541 agaggaggag acatgaagaa tcttgtgaac tgcctggcaa agcacctggg tgagctggtg
      601 ctggtgggcg gtgccgccgt ggtggcggtg ggcgcgggga tgatctatct gcccgcgggt
      661 ctgatcactg ggggcggcct ggccatcgcc ggcgcggtgc tgtccttgtg gggagcgggt
      721 gaggagaaat gagcctgcgg aaaggactgg cgcgggccgg gaagtcaagt gccgttcgga
      781 aagggctggc cggcgtcggc cggcttctga ccctggataa cccggagggc tggctgagcg
      841 gcgaggagct ggtcggcctg agccgggacc gggccatgaa gatctccaca gtcaaccggt
      901 gcgtggagct gctgtccacc tccatggcgg tgctgccagt ctacatcatg gaggagcgga
      961 ccaagaatcg gaggccggat caccatctgg gccgggtgct gtgggagcgg cccaacgagg
     1021 ccatgacccc cttcgacttc cggcggctgc tgatgtgcaa ccagctcctc cggggaaacg
     1081 cctacgcctg gatctaccgg gacgccgtga gcggcctgcc catggaactg atccccctgc
     1141 ccccggacta tgtatccatg cacctggacc tgtccggcaa ggtgtggtac ctcttcaccc
     1201 atccggtcac cggcgaggtg acccgcatcc ggtgcgagga tatgctgcac tacaaggcct
     1261 attcggaaga cggtctggag gggatcagcg tactgcgccg ggcctccctg accctggaca
     1321 ctgcccgggc ggcccagctg tatgagaaca gcatctggcg caacggaggc cagcccagcg
     1381 gcatcctgac cacggagaca gacctgggcg gtgagtacga ggtggatctg gaggacggca
     1441 ccacggtgaa gagagatccc aaggagcagt tgaggcagtc ctgggaggcg atccacagcg
     1501 gaccgggcaa cgcctttaaa gtggcggtgc tggacctggg cctgaagtat cagcctatct
     1561 ccatgaacaa caccgacgcc cagtttgtgg agagcagcga gatccgggtg gcggatgtgt
     1621 gccgattctt cggggtgccc ctgcacctgg cctacgcagg caagcagagt taccagagca
     1681 acgagcagaa cggcatcgag tatgtgacct acaccctgat gggctatgac acccaatggg
     1741 gacaggagga cacctacaag ctgttgctcc cgggggagcg ggccaaagga ctgcggatca
     1801 agcgggagat gaaggtattc ctgcggggcg ataccgccgc ccaggccgcc tggctcaaag
     1861 ccatgcgtga aatcggcgct tactgctcgg atgagatccg ggcgctggac gacctgccgg
     1921 ccatcccggg cggcaaggaa tactactcca gcctgaacta tgtccccctg gagctgtggc
     1981 gaatcctgag catcatccgg gcgctgggca aggcggccga cgtcccggag gagccgccgt
     2041 tgggcgagga cccggaggga gcggaagatc cccccgcgaa ggaaggagag aaagccaatg
     2101 aatgagatcc tgaaagccgc ccatgtggag aagcaggcgg tgagcgagga ggaactggcc
     2161 ctgatcaacg cccaaaccct gcgggcgctg accgccgacg aggtgttcac cttccgtttg
     2221 gcggcctgtg acgaccaggt ggaccgggac tatgaacggt ttaccctggc ggccctggag
     2281 ggcctggctc ccctgtttgt ggggcggccg gtgctgatgg accacaagtg gtccgccggt
     2341 acccagaccg cccggatcta cgccgccggc gtggaggaag ctgagaacgt ccaccggctg
     2401 gtcctgcggt gctatatgcc caggacagag cagacagctt ccacgatcac cgccattgag
     2461 agcggcatcc tgcgggagtg cagcgtgggc tgcgcggtgg agcgggcgct ctgctccatc
     2521 tgcggcgccg accaggtaca gacctgctgc cagcattggc cgggccggga gtacgacggc
     2581 aggctgtgcg taatggagct ggatggggca aaggacgcct acgaggtgtc gctggtggcg
     2641 gtcccggctc aaccgggcgc cgggatcgtg aaaagcaagc ggtacggcgg ccgggagtcc
     2701 cccgatgagc ccggggacga tgaagtgttt caactggcga tggccagaca ggaacaagaa
     2761 gaaatgcgat atggagggaa cgaactatga cgtatcagga atatctggag ctgaaggcca
     2821 agcgtgccgg gaagctgaag gagggcgagg cccttctggc caagaaggac tttgacggcc
     2881 acaaggccct gatgggtgaa gtggccaaga tgaaccagga gctggacgcc gctgaagccc
     2941 agctggcaga ggagggccgt tttgccgagg atgacgaggg tatgaagctg cgccacaagg
     3001 cattccaggc ccagaaagcg gacaaggcca agggcgcggc catcgacgag attcggagaa
     3061 gcaacgagta tgctgccgcc ttcgtcaagg ccctgcgcaa cggggtgaag gtcaacaagg
     3121 tgtggggcat ggagggctac gagcctctgg ccaaggccct gaccgagact ggcggctccc
     3181 ctgaaggggc ggacggcggc ttcctggtgc cccaggactt tgacgacatg atccacgagt
     3241 atgagaagga gtatgtggat ctgagccagt tcttcgctgt ggaaaatgta cgcagcctaa
     3301 gcggctggcg ggccgtggag aagggcaagc gcaaggctct gcccaagatc acggagatgg
     3361 gcaccatcgg caaggatgac cagcccaagt tcaccaaggt caccta
//
LOCUS       DBMDSJ010000164         3396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7036_length_3396_cov_1.720789, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000164 DBMDSJ010000000
VERSION     DBMDSJ010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7036_length_3396_cov_1.720789"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="ACZPAQ_05035"
     CDS             <1..405
                     /locus_tag="ACZPAQ_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903383.1"
                     /translation="EADEMIPVMLISGQMDVSDLANGFDSENLKGWGVNMLRVNGIGE
                     ELTRENANKVTNIDSRHPELYTWSKTTDGNEIPMVQWAQCLLRPHNCYPSDMPMLWDF
                     MEHFSFEKAADGTVTRYYSPSAFEKDDAVALN"
     gene            complement(523..1743)
                     /locus_tag="ACZPAQ_05040"
     CDS             complement(523..1743)
                     /locus_tag="ACZPAQ_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR0903384.1"
                     /translation="MERKKIELLAPAGDMERLQMAAAYGADAVYLAGTTFGMRSFAGN
                     FTPEALKEAVELCHRRGIRVHVTCNTMPRNNEVARLPEWLEYLDSLGVDAIILADVGT
                     LALAAAHAPHVERHISTQASVVNYQSARAWHQLGAKRVILARELSLDEIREIRDKTPP
                     ELELEVFAHGAMCVSYSGRCLLSNYMTGRDSNRGACAQPCRYQYALMEEKRPGEYFPV
                     YEENGETYIMNSRDMCMIDHVDELMDAGLDSLKIEGRAKSAYYAAIVTGAYRHAIDAA
                     WSGVPLDPVWRDEVEHISHRHYSTGFFYGQPGQFTEDSRYIRDWQITAKVVSCDQEGR
                     ALLTLNNKFSLGDRLELVGPGVRPQPLEVTALWDEDGLPLTQVRKPQMRFRMQLPQSV
                     PPLSILRRQADLTP"
     gene            complement(1838..2977)
                     /gene="mltG"
                     /locus_tag="ACZPAQ_05045"
     CDS             complement(1838..2977)
                     /gene="mltG"
                     /locus_tag="ACZPAQ_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR0903385.1"
                     /translation="MPQERRNSDYREAGRRSRSSSPAPRRRRSAARSASFAVLYAVAV
                     IGVSALLACVGWIAASDVLALNKEEHSVTVTITPEDSYDDVVDMLKDNGLIEYKSLFK
                     LFSAITGGKDKIASSGTFTLNSDMDYRALISGISANSASRAVVDVTIPEGYTVAQIFQ
                     LLEEKGVATVEDLNETAATHDYAFSFLQDIPLGDPERLEGYLFPATYEFYTPHNPLYA
                     INKMLQTFDAQFTDEMRQEVADSGRTIHEVLTVASMIEKETDGEDRGKIASVIYNRLN
                     NPGASAGTNGYLQIDATLAYLNGGKVPTEADKSIDSPYNTYLYPGLPPGPIANPGLES
                     IKAAMEPESTSYYYYTLGDDNKHHFFNTYGEMTSFMSTQALYSGG"
     gene            3100..3282
                     /locus_tag="ACZPAQ_05050"
     CDS             3100..3282
                     /locus_tag="ACZPAQ_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903386.1"
                     /translation="MCFGNNGCGGNSCLWIILILIIIWGCGGNSWGCNSNCGCNSCGC
                     GNNCGNSGCGCGDSCC"
BASE COUNT          642 a          954 c         1152 g          648 t
ORIGIN      
        1 gaagcagacg aaatgatacc ggttatgctg attagtggac agatggatgt cagcgatttg
       61 gcaaacggtt ttgattccga aaatctcaag ggatggggtg tcaacatgct gagagtgaac
      121 ggaatcggtg aagaactgac tagggaaaat gccaataaag tgaccaacat tgattcccgc
      181 catccagaac tgtatacttg gagcaaaaca actgatggca atgagattcc catggttcag
      241 tgggcgcagt gcctcctgcg tccccacaac tgctatccct ctgatatgcc tatgctgtgg
      301 gacttcatgg agcacttcag ctttgagaag gccgcggacg gcactgtgac ccgctattac
      361 agcccctccg cctttgaaaa ggacgatgcg gtggcgctga actgagagaa gcaaatttta
      421 aaaagacaac ggttcgcttc catctgtcag agaacaaaaa ggaaagcgca gggcttctcc
      481 ccacacgggg agaagccctg cgctcgtttg cacagaaaga actcaagggg tcaggtccgc
      541 ctgacggcgc aggatggaga gggggggcac cgactggggc agctgcatgc ggaagcgcat
      601 ctggggctta cgtacctggg taaggggcag gccgtcctcg tcccacagcg ccgtgacctc
      661 cagaggctgg gggcggacgc cggggcccac cagctccagc cggtcgccca gggaaaactt
      721 gttgttcagg gtgagcaggg cgcggccctc ctggtcacag ctgaccacct ttgcggtgat
      781 ctgccagtcc cggatgtagc gggaatcctc tgtgaactgg cctggctggc cgtaaaagaa
      841 accggtggag tagtgccggt ggctgatgtg ctctacctca tcccgccaga cggggtccag
      901 aggcacgccg ctccaggcgg cgtcgatggc gtggcggtaa gctccggtga cgatggcggc
      961 gtagtaagct gatttggccc gcccctcgat cttgagggag tccaggccgg cgtccatcag
     1021 ctcgtccacg tggtcgatca tgcacatatc ccgggagttc atgatgtagg tctccccatt
     1081 ctcctcatag acggggaagt attctcctgg ccgcttctcc tccatcaggg cgtactgata
     1141 gcggcagggc tgggcgcagg ccccccggtt ggagtcccgg cccgtcatgt agttggacag
     1201 caggcaccgg ccggagtagc tgacgcacat ggcgccgtgg gcaaagacct ccagctccag
     1261 ctcggggggc gtcttgtccc ggatctcccg gatctcgtcc aggctgagct cccgggccag
     1321 gatgacccgc ttggccccca gttggtgcca tgcccgggcg gactggtagt tgaccacact
     1381 ggcctgggtg gagatgtgcc gctccacatg gggggcgtgg gcggcggcca gagccagggt
     1441 gcccacatcg gccaggatga tggcgtccac ccccaggctg tccagatatt ccagccactc
     1501 cggcaggcgg gccacctcat tgtttcgggg catggtgttg caggtaacat ggacccggat
     1561 gccccggcgg tggcagagct ccaccgcctc cttcagtgct tccggcgtaa agttgccagc
     1621 aaaggagcgc atgccgaaag tagtaccggc cagatagacc gcgtctgccc cataggcggc
     1681 ggccatctga aggcgctcca tgtcgccggc cggagccagc agttcgatct tctttcgttc
     1741 catcaggatc agcctcccat cagagcggca taggagccgc gccttttcaa caggtcgagc
     1801 gggccccgga agtcgttccg gggcccgctg taaatcgtca gccgccgctg taaagcgcct
     1861 gggtggacat gaaggaggtc atctctccgt aagtgttgaa gaaatggtgc ttgttgtcgt
     1921 cgcccagggt ataataatag taggaggtgc tctccggctc catagcggcc ttgatggatt
     1981 ccaggccggg gttggcgatg gggccggggg gcaggccggg atacagatag gtattgtagg
     2041 gggagtcgat ggacttgtcc gcctcggtgg gcaccttgcc cccgttgagg taggccaggg
     2101 tggcgtcgat ctgaaggtag ccgttggtgc cggcggaggc accggggttg ttcaggcggt
     2161 tgtagatgac ggaggcgatc ttcccccggt cctcaccgtc agtctccttt tcgatcatgg
     2221 aggccacagt gagcacctcg tggatggtgc gtccgctgtc cgccacctcc tggcgcatct
     2281 catcggtgaa ctgggcgtcg aaggtctgaa gcatcttgtt gatggcgtac aggggattgt
     2341 ggggcgtgta gaactcgtag gtggcgggga acaggtagcc ctccagccgc tcggggtcac
     2401 ccaggggaat gtcctggagg aaggagaagg cgtagtcgtg ggtggccgcc gtctcgttca
     2461 ggtcctccac cgtggccacc cccttctcct ccaggagctg gaagatctgg gctaccgtgt
     2521 agccctccgg gatggtgaca tccaccactg cccgggaggc ggaattggcg ctgatgccgg
     2581 agatcagggc gcggtagtcc atgtcggagt tcagggtaaa ggtgccggaa gaggcgatct
     2641 tgtccttgcc gccggtgatg gcggagaaga gcttgaagag agatttgtac tcgatcaggc
     2701 cgttgtcctt gagcatatcc accacgtcgt catagctgtc ctccggggtg atggtgacgg
     2761 tgacggagtg ctcctccttg ttcagggcca gcacgtcgct ggcggcgatc cagcccacac
     2821 aggccagcag agcggatacg ccgatgacgg ccacagcgta gagcacagca aagctggcgg
     2881 agcgggcggc gctgcgccgg cgccgggggg cgggggagga ggaccggctg cgccggccgg
     2941 cctcccggta gtctgagttt ctgcgttcct gaggcatagg gtcagctcct aacaaaattt
     3001 ccttccccgt ggaaccgggg acaggggtgg gccatagaat ggtacagagg cgagagaccg
     3061 gcgacggtct cggccgactt tgagtgaggt gaatgtttca tgtgctttgg aaacaacggt
     3121 tgcggcggta acagctgtct gtggatcatc ctgatcctga tcatcatctg gggctgcggc
     3181 ggaaacagct ggggctgcaa cagtaactgc ggctgcaaca gctgcggctg cgggaacaac
     3241 tgcggcaaca gcggctgcgg ctgcggcgac agctgctgct gaggcggact acatccgctg
     3301 gagacaggcg gggccctccg gccctgcctt tttcctgcgg ggccggcgga caggcagggg
     3361 ctcagaagcg gaggacccgt cggtccgtca ccagaa
//
LOCUS       DBMDSJ010000165         3339 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7164_length_3339_cov_1.644534, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000165 DBMDSJ010000000
VERSION     DBMDSJ010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_3339_cov_1.644534"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            124..744
                     /locus_tag="ACZPAQ_05055"
     CDS             124..744
                     /locus_tag="ACZPAQ_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family nuclease-associated radical SAM
                     protein"
                     /protein_id="HIR0903387.1"
                     /translation="MTITYEYEDALYVNLTNKCNCNCEFCLRHGRKEGSIYTEDSLWL
                     EREPTRKEALDSFLSRDVCSYREIVFCGYGEPTYRLDDILWLVDQLKERFGRRLPPVR
                     INTNGHANLILGRDVVPELKGRIDTLSISLNAVTAAEYTALCHPAQGEAAYQAMLDFA
                     REATRYVPHVVLTIVDKDKSSEEIETCWQIAAELGVKLRIRSFIDS"
     gene            complement(1412..1588)
                     /locus_tag="ACZPAQ_05060"
     CDS             complement(1412..1588)
                     /locus_tag="ACZPAQ_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903388.1"
                     /translation="MKVARFVLKIVALSLTTAAVVCAVIAYWDKVAEFCGCAGEKLRK
                     KTGCPSEYDDYVEE"
     gene            complement(1695..2822)
                     /locus_tag="ACZPAQ_05065"
     CDS             complement(1695..2822)
                     /locus_tag="ACZPAQ_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HIR0903389.1"
                     /translation="MTKAYSIFLTVLFTVFIGGVLVISALLPKEEFSEMENRFLQKPP
                     ELSIETVKNGKFMENAEKYVSDHIAGRDLWVAAKAWGERLSGKQENNGVYFAAQDTLI
                     NRVEEPDGETWEQLDQNLEYLDQLVSNAGVPVYFGLIPSAAQVWHDRLPDGAPTAGEA
                     AIIETLYSRTAARTVDLLSVLSAHSGEELYYRTDHHWTSLGAYYGYTALMEAMGMEPV
                     DLADYERTTVTDQFYGTIFSASGVRWVAPDRIDTYIPADGVKVTSYFDGTPSQGSLYV
                     DSYLEVKDKYSYFLGGVQPLCVVETGLTDAPRLLLIRDSYSDSLTPFLTQSFSQIHLF
                     DPRYNLSSIQGYIQDNGIDAVAVLYSIPNYIADEYQFVLSR"
     gene            complement(2835..>3339)
                     /locus_tag="ACZPAQ_05070"
     CDS             complement(2835..>3339)
                     /locus_tag="ACZPAQ_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR0903390.1"
                     /translation="RCSKGRWVLNLFLVWAATGIWHGASWNFVLWGLYFWVLLLVEKF
                     VLLRWLKRAPGAVGHLYTLFFVLVSWTIFAIEDFGQLGEYLKVMFGLGGVPLIDGAFK
                     YYAANYLPVLCISALAATPLGAAVYRRLNVRTAHILGAVLILAGLLLCTAYLVDGTYN
                     PFLYFRF"
BASE COUNT          667 a         1030 c          968 g          674 t
ORIGIN      
        1 gaggtcgctg cggtgacgct ggaaaatggc cggcggtttt ttggcatttc ctaaggtgac
       61 gcatcaagca ggggccgcca gaacggcggc ccctgggcta ttttaaaaaa aggatgtttt
      121 ctcatgacca taacctacga gtatgaagac gccctctatg tgaatctcac caacaagtgc
      181 aactgcaact gcgaattctg cctgcgtcac ggccggaagg aaggctccat ttatacggag
      241 gactccctct ggctggaacg ggagcccacc cggaaggagg cgctggacag cttcctcagc
      301 cgggacgtct gctcctaccg ggagatcgtc ttctgcggct atggggagcc cacctaccgc
      361 ctggacgata tcctctggct ggtagaccag ctgaaggagc gtttcggccg aaggctgcca
      421 cctgtgcgca tcaataccaa cggccacgcc aacctgatcc tcgggcggga cgtggtgccc
      481 gagctgaagg gccgcatcga cacgctctcc atctccctca atgcggtcac tgctgcagaa
      541 tatacggctc tgtgccatcc tgctcaggga gaggcggcct atcaggctat gctggacttt
      601 gcccgggagg ccacccggta tgtcccccat gtggtgctta ccattgtgga caaggacaag
      661 agctcggagg agatcgagac ctgctggcag atcgccgctg agctgggggt gaagctgcgt
      721 atccgcagct tcatcgactc ctgaggtgtt gtccggccct gcacccatgc aataaaagag
      781 gctgtccatc ccgccggggt atggacagcc tcttttactg tgatttttac gccgtcagct
      841 ccagctcctc cagcgcttcc cgttcattgt cggccgagaa gcaaaaccgg ccggcccagt
      901 cgtagacctc cacgtggccc cttacccata cgatgttata atccatactg tcttgctcct
      961 ttccgttttc tgtgctctca gtatagcgga aaagaagtcc tataaaccgg acttttatcg
     1021 cggcggagga agagagtatt ttgttttttg caggacgtca cggggacatg gtcttacagg
     1081 actgtcctct cttgatgctc gcgcagggac agacttggtc cttcacgccg gtttcggtcc
     1141 ggcgcgggga aaggcccccc atccgtcacc gctcacgccg tgccaccttc ccccaggagg
     1201 ggggggaggg acttgagggc agctctggga gccgccctta tatggcacct tcagtcactt
     1261 cgtaggggcg cgcattgcga gcccgccagc ttcctaccgc cccctgttct gccagggcag
     1321 gcgcaatgcc gccgtggggt gtgcacgcca tgcgccccct cccaagcgca aaagaagatc
     1381 ggctgaactt gaaaagttca gccgatcttc tttactcctc tacgtagtcg tcatactcgg
     1441 agggacagcc ggttttcttt ctcagcttct ctccggcgca gccgcagaac tccgccacct
     1501 tatcccagta ggcgatgacc gcgcatacca cggcggcggt ggtaagcgac agggccacga
     1561 tcttcagtac gaaacgggcg accttcataa acgggatgcc tccttttccg gtgattcgtg
     1621 tctctagtgt acacgattct ttggaaaatt acaatcgttt ttccaaattt tcatgtgccc
     1681 ttttcttccg ggcatcagcg ggacagcacg aattgatact catcggcgat atagttgggg
     1741 atgctgtaca gcaccgccac cgcatcgatg ccgttgtcct ggatatagcc ctggatgctg
     1801 ctcaggttat acctggggtc aaacaggtgg atctgggaga agctctgagt caggaagggg
     1861 gtcagactgt cggagtagga gtcccggatc agcagcaggc ggggggcgtc ggtcaggccg
     1921 gtctccacca cgcacagggg ctggacaccc cccagaaagt aggagtattt gtcctttact
     1981 tccagatagc tgtccacata gaggcttccc tgggatgggg tcccgtcaaa ataggaggtc
     2041 accttcaccc catccgccgg gatgtaggtg tcgatccggt ccggggccac ccagcgcacc
     2101 ccggaggcgg agaagatggt cccatagaac tggtcggtca cagtcgtcct ctcatagtcc
     2161 gccagatcca ccggctccat ccccatggcc tccatcaggg cggtgtagcc gtaataggcg
     2221 cccaggctgg tccagtggtg gtcggtgcgg tagtagagct cctccccgct gtgggcggag
     2281 aggacggaga gcagatccac cgtgcgggcc gcggtccggc tgtaaagggt ctcgatgatg
     2341 gccgcctcgc cggcagtggg ggccccgtca gggagccggt cgtgccacac ctgggcggcg
     2401 gaggggatca ggccgaagta aaccggcacc ccggcgttgc tcaccagctg gtccagatac
     2461 tccagattct ggtccagctg ctcccaggtc tccccgtcag gctcctccac ccggttgatc
     2521 agggtgtcct gggccgcaaa atagaccccg ttgttctcct gcttgccgct gagccgctcc
     2581 ccccacgcct ttgcggctac ccagaggtcc cggcctgcga tgtggtcgct gacatacttc
     2641 tctgcattct ccatgaattt cccatttttc acggtctcta tgctcagctc cggcggcttc
     2701 tggaggaagc ggttctccat ctcagagaac tcctccttgg gcagcagggc gctgatcacc
     2761 aggacgcccc caatgaacac cgtgaagagt acggtgagaa aaatgctgta cgctttggtc
     2821 atgatatctc ctcctcagaa ccggaagtac aggaaggggt tataggtccc gtccaccaga
     2881 taggcagtgc acaacaacag ccctgccagg atcagtaccg cccccaggat atgggccgtg
     2941 cggacattca gcctgcggta gaccgccgcc cccagcgggg tggcagccag ggcagagatg
     3001 cagaggaccg gcagatagtt ggccgcgtag tacttgaaag ccccatcgat caggggcacg
     3061 cctcccagac cgaacatcac cttcaggtac tcccccagct gtccgaagtc ctcgatggca
     3121 aagatggtcc agcttaccag cacaaaaaac agggtgtaga ggtggcctac cgcaccgggg
     3181 gcccgcttca gccaccggag caggacaaat ttctccacca gcagcagtac ccaaaagtac
     3241 aatccccaga gcacgaagtt ccagctggcc ccgtgccaga tgccggtggc cgcccacacc
     3301 aggaacagat tcaggaccca ccgccccttg gagcagcgg
//
LOCUS       DBMDSJ010000166         3337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7171_length_3337_cov_2.135352, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000166 DBMDSJ010000000
VERSION     DBMDSJ010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7171_length_3337_cov_2.135352"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="ACZPAQ_05075"
     CDS             complement(<1..255)
                     /locus_tag="ACZPAQ_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982193.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HIR0903391.1"
                     /translation="MADNIGNKAAHDYHLDIAPVQEGFYVKGAAHCDWGMKNRLSRLF
                     DPKSGNTVMLAFDHGYIMGPTAGLERIDVVIPPLIPYVNVL"
     gene            complement(259..1257)
                     /locus_tag="ACZPAQ_05080"
     CDS             complement(259..1257)
                     /locus_tag="ACZPAQ_05080"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HIR0903392.1"
                     /translation="MKMKKFINSPETITDEELVGLGLAYPDILEVDGHLVISKDLADA
                     DRVTIVTYGGSGHEPAQAGFVGKGMLDVQAVGDIFAAPNGKLVFDAMKLADKGHGVLL
                     LTLNYAGDQLAGKQAIKLAQEAGLNFRQVVTGEEIQYDPSGEDNKRGLAGAVALYHIA
                     AAAAREGKSLDEVAEIAQRYADSMASVTVKSTDATHPQNGMSFGDLGETDLMEIGAGQ
                     HGEGGGVRVPMMSSKDTVATVAEALSKKLELKAGDKAFVMINGCGATTMMEMLVLFKD
                     TVEFLKAKGVEVVASMVGEILTVQEAAGFQMNIAKWDDEFIRLWNTPCHTPAYSKE"
     gene            complement(1297..1905)
                     /locus_tag="ACZPAQ_05085"
     CDS             complement(1297..1905)
                     /locus_tag="ACZPAQ_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR0903393.1"
                     /translation="MTKQEFASRLQNACAAVTAAEQELTEIDSRFGDADHGLTMSKIA
                     KAVSAAAGESEGGIQAMLDDAAMAVMVLNGGSAVPLWNTWLDGLQEEAPEGNEIDTAG
                     IQAIFAKALKALSDMSGAKVGDKTMMDALIPASQAIAAYSGSDEAGLFDAAAQAALQG
                     AEASKQFVSKFGRAKSYGEQTIGTPDAGAASMAYFFQGLARP"
     gene            complement(2121..3053)
                     /locus_tag="ACZPAQ_05090"
     CDS             complement(2121..3053)
                     /locus_tag="ACZPAQ_05090"
                     /inference="COORDINATES: protein motif:HMM:NF016114.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HIR0903394.1"
                     /translation="MAPSKEKLLRLAHVARRYYLEDWKQIDIARELGVSRPLVSRMLG
                     EARELGVVHITVYEPGEESAVLLDRLRLSTSLQGGVLVEDGRDDDATNQLLSQGAVDL
                     LRQIGARRLGVGWGHLIGQLVTWLEENPQPSSTVTDIFPLVGNASIPARNYQSNENVR
                     LMAQQLGAAPHFLYLPALPESLEEKQLLCSTEVYRQIQDQWVQMDTALVNIGNYPSSP
                     DFASLVRYGSLLQQQHTCGRLLVYYFNEAGTVIQSDQDFAIQIPLELLKQCPHIVGVC
                     SANTSEKALRGALRSGIFTHIAARAELVKSLLGQ"
BASE COUNT          589 a         1048 c         1061 g          639 t
ORIGIN      
        1 gagcacgttc acatagggga tcaggggagg gatcaccacg tcgatgcgct ccagcccggc
       61 agtggggccc atgatgtagc cgtggtcaaa ggccagcatc accgtgttgc ccgacttggg
      121 atcaaagagg cgggacagac ggtttttcat gccccagtcg cagtgggcgg cccccttcac
      181 atagaagccc tcctggaccg gggcgatgtc caggtgatag tcgtgggcgg ccttgtttcc
      241 gatgttgtca gccatgtctt actccttgct gtacgcaggg gtgtggcagg gggtgttcca
      301 caggcggatg aactcgtcgt cccacttggc gatgttcatc tggaagccgg cggcctcctg
      361 cacggtgagg atctctccca ccatgctggc caccacttcc acgcccttgg ctttcaggaa
      421 ctccacggtg tccttgaaca gcaccagcat ctccatcatg gtggtggcgc cgcagccgtt
      481 gatcatcacg aaggccttgt cgcccgcctt cagctccagc ttcttggaca gggcctcggc
      541 cacagtggcc acggtatcct tggaggacat catgggcacc cggacgccgc cgccctcgcc
      601 gtgctggccg gcaccgatct ccatcaggtc ggtctcaccc aggtcgccga aagacatgcc
      661 gttctggggg tgggtggcgt cggtggactt gacggtgaca gaggccatgc tgtcggcgta
      721 ccgctgggcg atctcggcca cctcgtccag gctcttgccc tcccgggcgg cggcggccgc
      781 aatgtgatac agggccaccg caccggccag gccccgcttg ttgtcctccc cgctggggtc
      841 gtactggatc tcctcgccgg tgaccacctg gcggaagttc agcccagcct cctgggccag
      901 cttgatggcc tgcttgcccg ccagctggtc gccggcgtag ttcagagtga gcagcagaac
      961 gccgtggccc ttgtcggcca gcttcatggc gtcaaagacc agcttgccgt tgggggcggc
     1021 gaagatatcg cccaccgcct gcacgtccag cattcccttg cccacaaaac ccgcctgggc
     1081 gggctcgtgg ccggagccgc cgtaggtgac gatggtcacc cggtctgcgt cggccaggtc
     1141 cttgctgatg actagatggc cgtccacctc cagaatgtcg gggtaggcca ggccaagacc
     1201 caccagctcc tcgtcggtga tggtctcggg ggagttgata aacttcttca ttttcatata
     1261 tggagtacct cccgttcaaa atcaatggag atgcgctcag ggccgtgcca gcccctggaa
     1321 gaaataggcc atggaggcgg ccccggcatc cggggtcccg atggtctgct cgccgtagct
     1381 cttggcacgg ccgaacttgg agacgaactg cttgctggcc tcggccccct ggagcgcggc
     1441 ctgggcggcg gcgtcgaaca gcccggcctc atcgctgccg gagtaggcag cgatggcctg
     1501 actggcgggg atcagggcgt ccatcatggt cttgtccccc accttggcgc cggacatgtc
     1561 gctgagggcc ttcagcgctt tggcaaagat ggcctggatg cccgccgtat cgatctcatt
     1621 cccctcgggg gcctcctcct ggagaccatc cagccaggtg ttccacaggg ggacggcact
     1681 gccgccgttc aggaccatga cagccatggc cgcatcgtcc agcatggctt ggatgccgcc
     1741 ctctgactcc cctgctgcgg cggagacggc tttggcgatc ttgctcatgg tcaggccgtg
     1801 gtcggcgtcg ccgaaccggg agtcgatctc cgtcagctcc tgctctgcgg cggtcactgc
     1861 cgcacaggcg ttctgaagcc gggatgcaaa ttcctgcttg gtcatatggt gtcacctccc
     1921 tcctggacaa aacttaggaa agctcctgct gggctgtgtt acttctgtgc atagatacta
     1981 ctataaaagg taacaaaaat caatagatag atcttttttc tctatcattc acgtaatgat
     2041 atattgaaat ttatacaaaa cagacaaaaa tagaactccc tgtggaagaa cgatcctcca
     2101 caggaagtta catatgttac ttattggccc agcagggact tgaccagctc cgcccgggct
     2161 gcgatgtggg tgaagatccc ggaccgcagg gcgccccgca gggctttctc actggtgttg
     2221 gcggagcaga cgcccacgat atgagggcac tgcttcagca gctccagagg gatctggatg
     2281 gcgaagtcct gatccgactg gatcaccgtg cctgcctcgt tgaagtagta gaccagcagc
     2341 cgtccacagg tgtgctgctg ctggagcagg ctgccgtacc ggaccaggga ggcaaagtcg
     2401 gggctggagg ggtagtttcc gatgttgacc agggcggtgt ccatctgaac ccactggtcc
     2461 tggatctggc ggtagacctc ggtggagcac agcagctgct tctcctccag gctctccggc
     2521 agggcgggga gatagaggaa gtggggggcg gcgcccagct gctgggccat gaggcggacg
     2581 ttttcgttgg actggtagtt gcgggcaggg atgctggcat ttcccaccag ggggaagata
     2641 tcagtgacgg tgctgctggg ctggggattt tcctccagcc aggtcaccag ctggccgatc
     2701 aggtggcccc agcctacccc caggcggcgg gcgccgatct gccgcagcag gtccacggcc
     2761 ccctgggaga gcagctgatt ggtggcgtcg tcgtcccggc cgtcctccac cagcacgccc
     2821 ccctggaggg aggtggacag ccgcagccgg tccagcagga cagcgctctc ttcccccggt
     2881 tcgtagacgg tgatatgcac cacgcccagc tcccgggcct cccccagcat ccggctcacc
     2941 aggggacggg acacccccag ttcccgggca atgtcgatct gcttccagtc ctccagatag
     3001 tagcgccggg ccacatgggc cagacggagg agcttttcct tgctgggcgc caagccgctc
     3061 acctgccttt ctacaccgcg gctgttacat aagtcaactg taccatatcg gcataacaaa
     3121 tgcaacggga agggggaaga aaatccgccc ggcagggaag ggcctctgcc ggacggggga
     3181 aatcaggggg acaccagagc gttgagcacc cgggaggcca catctccggc cgcggaggag
     3241 ccgctcccgc cgttctccac cagcacgaga aaggcgtagg ggtggtcctc atcccggagg
     3301 aagccggtga accaggcgtg gggggcctgg ccgcccc
//
LOCUS       DBMDSJ010000167         3334 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7181_length_3334_cov_1.461182, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000167 DBMDSJ010000000
VERSION     DBMDSJ010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_3334_cov_1.461182"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="ACZPAQ_05095"
     CDS             <1..999
                     /locus_tag="ACZPAQ_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR0903395.1"
                     /translation="YEEKNRQFTQEIGEKTARLQELRAENQVRSEEIRALGEEKLALE
                     GQRNQADKDSRAKNDELLAMEREVSILEQRKVAAAMEEKQLLDKLWETYELSHEAAKQ
                     HRVEIESVPKASRRIGELKRAISSLGSINLGAIEEYQRVNERYTYLTDQRDDVQRAKG
                     ELEEIIADITGEMKTIFAREFQTIDQAFGQTFTELFGGGKATLELEDPEDILNCGIEI
                     KVQPPGKALKIITLLSGGEKAFVAIALYFAILKVRPTPFVVMDEIEAALDDNNVARFA
                     HYMRSMADKTQFIVITHRRGTMEEADVLYGVTMQEQGVSRMLTINLNDVEKELKIR"
     gene            1331..1969
                     /locus_tag="ACZPAQ_05100"
     CDS             1331..1969
                     /locus_tag="ACZPAQ_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903396.1"
                     /translation="MKEKEKGGWLPIVGLILLIAVLFAGVKLVFAGLGYVIPQDEGPE
                     ETGTWYQMYNEQGEAVRYTLREPGEIDLSAPDIAQWMEQAAGELEQTPETEGIVFWLY
                     RQDTDEYLLYLPDQDRSLLEGGLTASEETEEDSEVCLVLRARTPENSKPVAPEETLYC
                     FATTSDSWNGIRVRVILDGREQEVRKLTSIGDRLYSTEETYIGRDRLEGSGT"
     gene            1966..2280
                     /locus_tag="ACZPAQ_05105"
     CDS             1966..2280
                     /locus_tag="ACZPAQ_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903397.1"
                     /translation="MSRRTLRCLRCGGTMELLRREFLQLGKTGWVLGDLDNLLAGALD
                     VDILCCSDCGKLEFFRGEWSELEDPEGSGIARMECPSCGRSYELDSPKCPFCGKKNTR
                     LF"
     gene            2296..3165
                     /gene="ftsY"
                     /locus_tag="ACZPAQ_05110"
     CDS             2296..3165
                     /gene="ftsY"
                     /locus_tag="ACZPAQ_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR0903398.1"
                     /translation="MGFFDKLKKINIFASFGSELTDDFYDELEESLILADTGMDTTLE
                     LVEELRRRVREERIKTVEEARTCMCRCIAGAMQVGDSALDLSTKPSVVLFIGVNGVGK
                     TTTIGKIGAQLKKERKKVLLCAADTFRAAAAEQLTIWADRSGLEIIRQHEGADPAAVV
                     FDAASAAKARNTDVVLVDTAGRLHNKQNLMNELNKISRVIDRELPGCAKETLLVLDAT
                     TGQNGLIQAKQFREAAGITGIVLTKLDGTAKGGIAIAIARELGVPVKYAGVGEGIDDL
                     RPFDALEFAQALI"
BASE COUNT          728 a          920 c         1147 g          539 t
ORIGIN      
        1 tatgaggaga agaaccgcca gttcacccag gaaatcgggg agaagacagc acgcctccag
       61 gagctgcggg cggagaatca ggtccgcagc gaggagatcc gcgccctggg cgaggaaaag
      121 ctggccctgg agggacagcg caaccaggcg gacaaagaca gcagggccaa gaacgacgag
      181 ctgctggcca tggagcggga ggtctccata ctggagcagc ggaaggtggc tgccgctatg
      241 gaggagaagc agcttttgga taagctgtgg gagacctacg agctgtccca cgaggcggcc
      301 aagcagcacc gggtggagat cgaatcggtg cccaaggcca gccggcgcat cggggagctg
      361 aagcgggcca tctccagcct gggctccata aatttggggg ccatcgagga gtatcagcgg
      421 gtcaacgaac gatacaccta cctcaccgac cagagagacg atgtccagcg ggccaagggg
      481 gagctggagg agatcatcgc ggacatcacc ggggagatga agaccatctt cgcccgggag
      541 ttccagacca tcgaccaggc attcgggcag accttcacag agctcttcgg gggcggcaag
      601 gccacgctgg agctggagga cccggaggac atcctcaact gcggcatcga gatcaaagtg
      661 cagcccccgg gcaaggcgct gaagatcatc accctgctct ccggcgggga aaaggcattc
      721 gtggccatcg ccctgtattt cgccattttg aaggtccggc ccaccccctt cgtggtgatg
      781 gacgagatcg aggccgccct ggatgacaac aatgtggccc ggttcgccca ttacatgcgt
      841 tccatggcgg acaagaccca gtttatcgtc atcacccacc ggcgcggcac tatggaggag
      901 gccgacgtcc tctacggcgt caccatgcag gagcagggcg tgagccgtat gctgacgatc
      961 aatctcaacg acgtggagaa agagctgaag atccgatgaa cttcctttcc ctgctcctgc
     1021 atggcgggac cccaggccgg gcccagcgaa gcgggccgga ctgggagagg aggcgcaagg
     1081 gagcgggcga gggatgcccg gaggggcgtc ccgagcagaa gcggactttg caccgacgaa
     1141 ggagcagggc gtgagccgta tgctgacgat caatctcaac gacgtggaga aagagctgaa
     1201 gatccggtga gatctcattc ctccccctga atgaggacga ttttctggat cactgggcct
     1261 atatcgaccc caatactgcc cgctgggcgg agaacagata ttttacggca tagaaccggg
     1321 gaggatacat ttgaaggaga aggaaaaggg cgggtggctg cccattgtgg ggctgatcct
     1381 gctgatcgcg gtcctttttg cgggggtgaa gctggtattc gcggggctgg gatatgttat
     1441 cccccaggac gaaggtcccg aagagacagg gacttggtac cagatgtaca acgagcaggg
     1501 ggaggcggtg cgctacactc tgcgggagcc gggagagatc gacctgtccg cgccggacat
     1561 cgcccagtgg atggagcagg cggccggaga actggagcag accccggaga cagaagggat
     1621 tgtcttctgg ctctaccgcc aggacaccga cgagtatctg ctctatctgc cggaccagga
     1681 ccgttccctg ctggagggcg ggctgaccgc ctccgaagag acggaggagg acagtgaggt
     1741 gtgcctggtc ctgcgggccc gcaccccgga gaacagcaag ccggtggcgc cggaggagac
     1801 gctgtactgc ttcgccacta cctccgacag ctggaacgga atccgggtcc gagtcatcct
     1861 ggacggccgg gagcaggagg tccgcaagct gacctccatt ggagaccggc tgtactccac
     1921 ggaggagacc tatatcggcc gggaccgact ggaagggagc gggacatgag ccggaggacc
     1981 ctgagatgcc tgcgctgcgg cgggacgatg gagctgctgc ggcgggaatt tctccagctg
     2041 ggaaagacag ggtgggtcct gggagatctg gacaacctgc tggccggggc gctggatgtg
     2101 gatatcctgt gctgttcaga ctgcgggaag ctggaatttt tccggggaga gtggtctgag
     2161 ctggaggacc cggagggcag cggcatcgcc cggatggagt gtcccagctg cggcaggagc
     2221 tatgagctgg acagccccaa gtgccccttc tgcggaaaga agaataccag attgttttaa
     2281 gaaaggatca aacgtatggg cttttttgac aagctgaaaa agatcaatat ttttgccagc
     2341 ttcggttcgg agctgaccga cgacttttac gatgagctgg aggagtccct gatcctggcg
     2401 gacaccggca tggacaccac cctggagctg gtggaggaac tgcgccgccg ggtgcgggag
     2461 gagcgcatca agacggtgga ggaggcccgg acctgcatgt gccggtgtat cgccggcgcc
     2521 atgcaggtgg gcgactcagc cctggacctg tccaccaagc cttcggtggt actgttcatc
     2581 ggggtgaacg gggtgggcaa gaccaccacc atcggcaaga tcggggcaca gctgaagaag
     2641 gagcggaaaa aggtgctgct gtgcgccgcc gacacattca gggccgccgc cgcagagcag
     2701 ctgaccatct gggcggaccg ctccggcctg gagatcatca ggcagcacga gggcgcggac
     2761 ccggcggctg tggtctttga cgctgcctcc gccgccaagg cccggaacac cgacgtggtg
     2821 ctggtggata ccgccgggcg gctgcacaac aaacagaacc tgatgaacga gctgaacaag
     2881 atctcccggg tcatcgaccg ggagctgccg ggctgcgcca aggagaccct gctggtgctg
     2941 gacgccacca cggggcagaa cggactgatc caggccaagc agttccggga ggccgccggc
     3001 atcaccggca tcgtcctcac caagctggac ggcaccgcca aggggggcat cgccatcgcc
     3061 atcgcccggg agctgggcgt gcctgtgaag tacgccgggg taggcgaggg gatcgacgac
     3121 ctcagaccct ttgacgcgct ggagtttgcg caggcgctca tttgagttag ggattaggaa
     3181 ttgggagccg gtcgtcctgg cgctcgccgt tgtaagacca tgtcatcggc gggacagctg
     3241 ccgcctcaga gttaagaaaa attaagagtc atattcatat ctatttcaac atttttcctc
     3301 agcgatatgg taaaataaaa tgacgtctcc cgga
//
LOCUS       DBMDSJ010000168         3328 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7194_length_3328_cov_1.879764, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000168 DBMDSJ010000000
VERSION     DBMDSJ010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7194_length_3328_cov_1.879764"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="ACZPAQ_05115"
     CDS             <1..675
                     /locus_tag="ACZPAQ_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903399.1"
                     /translation="YQKEKGGVFMGSRAADWDGELVLSYRDEPLIIRTVGESNGRYTT
                     TSIRVRLAIHLERDYELRIRPKSAMSTGLNTVLGVLDRGLEKLPSGPDLHEDYGCPEV
                     TRGRTITTTDRSFTKLVLRDLELRNALLASPKDGLLILPGPGREGLHLVESYSYPDSM
                     YTDWYSEPMNTGPDWTDTEEERAEKEEKLAELAQDTFFPRLEKVIGLTKAARGAALTW
                     RMGTPG"
     gene            848..1936
                     /locus_tag="ACZPAQ_05120"
     CDS             848..1936
                     /locus_tag="ACZPAQ_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HIR0903400.1"
                     /translation="MEKLRAFLKRKNIEISAKRYGIDALGAMAQGLFCSLLIGTIVKT
                     LGQQLGMEFLIEVGGYASAMSGAAMAVAIGYALKAPPLVLFSLVTVGYAANALGSVTL
                     SGGSGAGGPLAVLFIAVIAAELGKAVSKETKIDILVTPLVTILAGVALSAWWAPAIGT
                     AASAVGDFVKWATVLQPFWMGILVSVVIGVALTLPISSAAICAAIGLTGLAGGAAVAG
                     CCANMVGFAVLSFRENRWSGLISQGLGTSMLQMGNIVRNPRIWLPAILASAVTGPLAT
                     CVFQFEMNDPAGGVAAGMGTCGMVGPIGVYAGWVNDVATGAKAAITGMDWLAMALICF
                     VLPAVLAWLFGLFFRRIGWIREGDLTLE"
     gene            2177..2611
                     /locus_tag="ACZPAQ_05125"
     CDS             2177..2611
                     /locus_tag="ACZPAQ_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR0903401.1"
                     /translation="MNSDFCVAVHALVYLNRRGCVLSSEELARNICTNPARVRKVLAR
                     MKKDGLVETKEGSSGGGYRFAKGAGEVSLAQVARALEVRFVSTSWHSGSQEVKCLVAT
                     GMAQVMDALFDDLDDRCMARLEEITVEQVDRKLSGGPCPGGG"
     gene            2648..>3328
                     /locus_tag="ACZPAQ_05130"
     CDS             2648..>3328
                     /locus_tag="ACZPAQ_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR0903402.1"
                     /translation="MGFDLEISVPALTVFIQGILSFFSPCVLPLVPLYVGYLAGGAKT
                     VEVDGTIRYPRGRVMWNTLFFVLGISATFFLLGFGFTALGQFFSDNRLWFARISGIIM
                     VLFGLYQLGVFGHSMALEREHRLPFRLGGRTMGPLVALVLGFTFSFAWTPCVGPVLGS
                     VLLMASSSASAPAAALLIGVYTLGFVLPFLAVGLFTGGVLSFFKRHQQVVRWTGKAGA
                     ALLILMGVM"
BASE COUNT          511 a          987 c         1180 g          650 t
ORIGIN      
        1 tatcaaaagg agaagggggg cgtatttatg ggcagccggg cagcagactg ggacggggag
       61 ctggtgctga gctaccggga cgaaccgctc atcatccgga cggtggggga gagcaatggc
      121 cggtacacta cgacctccat ccgcgtccgg ctggcgatcc atctggagcg ggactatgag
      181 ctgcgcatcc gccccaaaag tgccatgtcc accggcctca acacggtgct gggcgtcctg
      241 gaccggggac tggagaagct gccctccgga ccggacctcc acgaggacta tggctgcccg
      301 gaggtgaccc ggggccggac catcaccacc acggaccggt cctttaccaa gctggtgctg
      361 cgggacctgg aactgcgcaa tgccctgctt gcctccccca aggacggcct gctcattctg
      421 cccgggccgg ggagggaggg gctgcacctg gtggagagtt actcctatcc ggactccatg
      481 tacacagatt ggtactcgga gccgatgaat accgggccgg actggacgga tacagaggag
      541 gagcgcgccg agaaggaaga gaagctggcg gagctggccc aggatacctt ttttccccgg
      601 ctggaaaagg tgatcggcct gaccaaggct gcccgggggg cggccctgac ctggaggatg
      661 gggacgccgg gatgattcga aggagcggct gtggtgagct gcggccgggc ggggcttgac
      721 tgccggaggg aaaagttttc gttgacaaac aggacagagc cgctataata ggccatacaa
      781 gtgtcttgac acctgtatgg cctttttgcc ggcaggtgcg ggactatcac gaaaaaaggt
      841 gagagaaatg gaaaagctcc gcgcgttttt aaagcgcaaa aacattgaga tctcggccaa
      901 gcggtatggc atcgacgccc tgggggccat ggcccagggc ctgttctgct ccctgctcat
      961 cggcaccatc gtcaagacgc tgggccagca gctggggatg gagttcctca tcgaagtggg
     1021 gggatacgcc tcggccatga gcggggcggc tatggcggtg gccatcggct acgccctgaa
     1081 ggcccccccg ctggtcctct tttccctggt cacggtgggc tatgcggcca atgccctggg
     1141 cagcgtcacc ctgtccgggg gcagcggcgc cggaggccct ctggcggtgc tgtttatcgc
     1201 ggtcatcgcc gcggagctgg gcaaggcggt gtctaaggag accaagatcg acatcctggt
     1261 cacccctctg gtgaccatcc tggcgggggt ggccctgtcg gcctggtggg ccccggccat
     1321 cggcacggcg gcctccgcgg tgggggactt cgtcaagtgg gccactgtgc tccagccctt
     1381 ctggatgggc atcctggtgt cggtggtcat cggcgtggcc ctgaccctgc ccatctcctc
     1441 tgccgccatc tgcgccgcca tcgggctgac cggcctggcc ggcggggccg cagtggcggg
     1501 gtgctgcgcc aacatggtgg gcttcgccgt cctgtccttc cgggagaacc gctggagcgg
     1561 cctgatctcc caggggctgg gcacctccat gctccagatg ggcaacatcg tccgcaatcc
     1621 ccggatctgg ctccccgcca tcctcgcctc cgcggtcacc ggccccctgg ccacctgtgt
     1681 gttccagttt gagatgaatg accccgccgg gggtgtggcg gctggaatgg ggacctgcgg
     1741 tatggtgggc cccatcgggg tctatgccgg ctgggtgaac gatgtggcca cgggggccaa
     1801 ggctgccatc accggcatgg actggctggc catggcgctg atctgctttg tactgcccgc
     1861 cgtcctcgct tggctgttcg ggctgttttt ccgtcggatc ggctggatcc gggaggggga
     1921 cctgaccctg gagtgagccg ggggagattt gcaggaccgt ttgggcggcc ggcacgcttg
     1981 cgtgccggcc gcctgtccgt ccctggcgac cgcctgctcc gcaggtccct ggccagtttc
     2041 ctggtgatgg gccgggtgtg ctgctggcgg caggattggc agggcctgtc cctgatggtg
     2101 ggacgaacac cctcttgaca gcctcttctg tatctgataa agttacagaa aacggctccc
     2161 tgggaggaga accattatga acagcgattt ctgcgtggcg gtccacgctc tggtctatct
     2221 caaccgaaga gggtgcgtcc tgtccagcga ggagctggcc cggaacatct gcactaaccc
     2281 cgcccgggtg cggaaggtgc tcgcccgtat gaaaaaggac ggcctggtgg agaccaagga
     2341 gggcagctcc ggcggaggct accgctttgc caagggggcg ggggaggtgt ccctggccca
     2401 ggtggcccgg gcgctggagg tgcgcttcgt ctccacctcc tggcacagcg gcagccagga
     2461 ggtgaagtgt ctggtggcca ccggcatggc tcaggtgatg gatgcccttt ttgatgacct
     2521 ggacgaccgg tgcatggccc ggctggagga gatcacagtg gaacaggtgg accggaagct
     2581 ctccggcgga ccctgccccg gcggcggatg atcccgccgc agagaacagt gcgaatggag
     2641 gttttttatg gggtttgatc tggagatcag cgttccggcg ctgacggttt tcatacaggg
     2701 catcctcagc ttcttctccc cctgcgtcct gcctctggtc cccttatatg tgggctatct
     2761 ggcaggcggg gccaagacgg tggaggtgga cggcaccatc cgctacccca gggggcgggt
     2821 gatgtggaac accctgtttt ttgtgctggg gatcagcgcc acctttttcc tgctgggctt
     2881 cggcttcacc gctttggggc agtttttcag cgataaccgg ctgtggtttg cccggatcag
     2941 cggcattatc atggtgctgt tcgggctgta ccagctgggg gtgtttggac attccatggc
     3001 gctggagcgg gagcacaggc tgcccttccg gctgggcggc cggaccatgg ggccgctggt
     3061 ggccctggtc ctgggcttta ccttcagttt cgcctggacc ccctgtgtag ggccggtgct
     3121 ggggagcgtg ctcctgatgg cctcatcctc ggcttcggcc ccggcagcgg cgctgctcat
     3181 cggggtgtac accctgggct ttgtgctgcc cttcctagcg gtggggctgt tcaccggcgg
     3241 ggtgctgtcc ttcttcaagc ggcaccagca ggtggtgcgc tggacgggga aggcgggggc
     3301 ggctctgctg atcctgatgg gggtgatg
//
LOCUS       DBMDSJ010000169         3327 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7196_length_3327_cov_1.522009, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000169 DBMDSJ010000000
VERSION     DBMDSJ010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7196_length_3327_cov_1.522009"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="ACZPAQ_05135"
     CDS             <1..1171
                     /locus_tag="ACZPAQ_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903403.1"
                     /translation="AQEVPGADLCIQDPDLEGFRMGGDGQPLFYLTARSDDREDSAAD
                     AVSGAEHLYIWSAGTFTRYNPYDLHRLPPLVPAEECLDLDPPLWRQWHFAGEKPEGGF
                     TSLALPVSPGTPRPPALTEEAEQMVLDYFASGRFRPVSGRAWFLGEGLSPQAGDLVIQ
                     DIQPAALYPTYETLCGIYRIRYLLYGSGDRLTDPHDLEDYLFFDLDQSENFLGVRTVM
                     SVPGGHLLTAALTDSWEGVLSDVYAALWRDGFPMPAGLWSSEISFYGEEDRPVHTVLK
                     SWRGSDSSDSLQLEQLSWEGFTSVTYCQPEYDRRSVWYLETTDPAFYTARGIRVGSTR
                     AEVENAYPTLQDPPFNGQEFPGVWYSSQEGRLKPAILFFFEDGLVSRIAVDFRTH"
     gene            1314..2255
                     /locus_tag="ACZPAQ_05140"
     CDS             1314..2255
                     /locus_tag="ACZPAQ_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR0903404.1"
                     /translation="MQLDIGMVFSSLIGLFLLMGVGWVSVKTKIVPPSASAPMSSLLL
                     KVTLPCTIFSSLATREFDSAFLVDGVIIIVIGLVLFPLYAALSWGISKVLKVPAGRRG
                     IWSFGATFCNNGFMGFPVALALFGEEGLALAVMFGVPFNLLVYTLGVRMVCADCGTDA
                     QPVNLVRTLLSNINIALILSLIFYVGQIPLPEALMTPVTHLSNVTTPLSMFVTGMTLA
                     SGKGTELFRDKDAYTATGMRLIVVPLLTWAVMSLLPLSNPLIASIVLITMAMPAPSVT
                     TILAETYNGNRGLAAKIVFLTSLFCMITLPLVAMFLL"
     gene            2507..2614
                     /locus_tag="ACZPAQ_05145"
                     /pseudo
     CDS             2507..2614
                     /locus_tag="ACZPAQ_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            3090..>3327
                     /locus_tag="ACZPAQ_05150"
     CDS             3090..>3327
                     /locus_tag="ACZPAQ_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903405.1"
                     /translation="MIYSGHNSPDLQEVHDGKIVNEQQRDTSLEALRENFDFGQVIAT
                     FLDRTLGHLVRGLFPEKQSEQVRALDHAIGFEVEV"
BASE COUNT          617 a         1046 c          918 g          746 t
ORIGIN      
        1 ggcccaggag gtccccgggg ccgacctctg catccaggac ccggacctgg agggcttccg
       61 catgggtggg gacggccagc ccctcttcta cctcaccgcc cgctccgacg accgggagga
      121 cagcgcggcg gacgccgtca gcggggcgga gcacctctat atctggtccg ctgggacctt
      181 cacccgctat aatccgtacg acctccaccg cctgccccct ctggtcccag cggaggagtg
      241 cctggacctg gacccgcccc tgtggcggca atggcacttc gcgggcgaga agccggaggg
      301 cggcttcacc tccctcgccc tccccgtctc ccccggcacg ccccgccctc cagcgctcac
      361 ggaggaggcg gagcagatgg tgctggacta cttcgcctca ggccgcttcc ggccggtctc
      421 aggtcgggcc tggttcctgg gggaggggct ctccccccag gccggcgacc tggtaatcca
      481 ggacatccag cccgccgccc tctaccccac ctacgagacc ctgtgcggga tctaccgcat
      541 ccgctatctg ctgtacggca gcggggaccg tctgaccgat ccgcacgatt tggaggacta
      601 cctctttttt gatctggacc agtcggagaa ctttcttggg gtgcgcaccg tcatgtcggt
      661 gccgggcggt catctcctca ccgccgccct gacggattcc tgggagggcg tgctgtccga
      721 cgtgtacgcc gccctctggc gggacggctt ccccatgccc gccggcctct ggtccagcga
      781 gatctccttt tacggggagg aggaccgtcc cgtccacacc gtcctgaaga gctggcgggg
      841 cagcgactcc agcgacagcc tccagctcga gcagctgagc tgggagggct ttacctccgt
      901 cacctactgc cagccggagt atgaccgccg ttcagtctgg tatctggaga ccactgaccc
      961 ggccttttac accgcccggg gcatccgagt gggctccacc cgcgcggagg tggagaacgc
     1021 ctaccccacg ctccaggatc cccccttcaa cggccaggag tttccgggcg tctggtacag
     1081 ctcccaggag gggcgcctca agccggccat tctctttttc tttgaagacg gcctggtcag
     1141 ccgcatcgcg gtggatttca ggacccactg agtactggca tgtctgtttt tcatagatcc
     1201 aggaaaaatc ttcacgctcc cggccccggt atctctggac agccctcctc ttctttgata
     1261 aactaggctt acagaaagaa cgccggccat cggccggccc gggaggggtt cctatgcagc
     1321 tagacatcgg aatggtattt tccagcttga tcggcctgtt tcttctcatg ggcgtgggat
     1381 gggtctccgt aaagacaaag attgtccccc cctccgcctc cgcccccatg tccagccttt
     1441 tgctgaaggt caccctgccc tgcaccatct tctcctccct ggccacccgg gagtttgact
     1501 ccgccttcct ggtggacggg gtcatcatca tcgtcatcgg actggtcctc ttccccctgt
     1561 acgctgccct gtcctggggc atctccaagg tgctgaaggt accggcgggc cggcggggca
     1621 tctggtcctt cggtgccacc ttctgcaaca acggctttat gggcttcccc gtggccctgg
     1681 ccctgtttgg cgaggagggc ctggccctgg ccgtcatgtt cggcgtaccc ttcaacctgc
     1741 tggtctatac cctgggtgtg cggatggtgt gcgctgactg cggcaccgac gcccagcctg
     1801 tcaatttggt ccgcaccctg ctgagcaaca tcaacatcgc ccttatcctc agcctcatct
     1861 tctatgtggg gcagatcccc ctgccggagg ccctgatgac ccccgtcacc catctgtcca
     1921 acgtgaccac gcccctgtcc atgttcgtca ctggcatgac cctggcctcc ggcaagggga
     1981 ctgagctgtt ccgggacaag gatgcctaca ccgccaccgg tatgcggctc atcgttgtcc
     2041 ccctgctcac ctgggcggtg atgtccctgc tccccctgtc taacccgctg atcgcctcca
     2101 tcgtcctcat caccatggcc atgcccgccc cctcggtcac caccattctg gcggagacct
     2161 acaacggcaa ccggggcctt gccgccaaga tcgtgttcct caccagtctg ttctgcatga
     2221 taaccctgcc cctagtggcc atgttcctcc tgtgatcctg ctgttcccgt gctgccgtcc
     2281 ccctgggacg gcagcacttt tttctccatg gagagcctcc gtcaaggcgg agaccctcca
     2341 tgtgtctctc atatcaaagc aaaaaagcct cctgccgcaa ggcgctctcg cgccggatct
     2401 tacgactctg cccgcaaagt ccttccgcaa atttttgcat aggcaaccag attttgcgga
     2461 tactactctc tgtctacaaa atgcaaccgc aggaggaatt tgatatatga aagccactgg
     2521 aatcgtacgg cgcatcgatg acttgggccg ggtggtcatc cccaaggaga tccgccgcac
     2581 gatgcgtatt cgggaaggtg acccgtcaca gatgacatgg acatggtggg aggggttctg
     2641 ttttggtatg ctggctttga ataatcgcct ggggttttag cggtgcacag cgggaaaaga
     2701 cgttcttccg acggtacata ttgggggata aaaatactgc acaaattgag tttgtgcagt
     2761 gattacacgg ataagggtta aaaatatctc catattgaca cactaatcct ggttatatat
     2821 gttccctaat ctaatacagt atcttgatat gactgtgatt gggaaggaac ctgtcttggg
     2881 tatcggcggg aatgtgccga attcggcagc ctgaatggac tttgttgcgg aggctgctgg
     2941 ggcataaaaa cagaaaatcc tacgcagcga tggaggacag aagttgatga gttggtctgc
     3001 acgattgggc tttttatact gtctgtatat cttcgcaaac atgaaagaag caaaaatgaa
     3061 cttagaatga cactatatgt cttcctagat tgatatactc cggccataac agtcccgatc
     3121 ttcaggaggt tcatgatgga aaaattgtta atgaacagca aagagatact tctctcgagg
     3181 cgctgcgcga aaacttcgat ttcggtcaag ttattgcaac ctttttagat cgcactctgg
     3241 ggcacttggt tagaggatta tttccagaaa aacagtctga acaggtacga gcattagacc
     3301 atgctattgg gtttgaggtt gaggtgg
//
LOCUS       DBMDSJ010000170         3303 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7255_length_3303_cov_1.793254, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000170 DBMDSJ010000000
VERSION     DBMDSJ010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7255_length_3303_cov_1.793254"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="ACZPAQ_05155"
     CDS             <1..1263
                     /locus_tag="ACZPAQ_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903406.1"
                     /translation="LEGLAQEGCNPWQTEGLPAAWQIRALTPADGRYLLSSPAVSEEA
                     DYLLTAQADSDPELCLTARLRLRHGTGEVPQAVEWTIGTPQELGLDGEPPRCPLPLSD
                     GRTLTLEFDESPIDGMESTVLIRQVKVWDGPVLLQSITAGSVLEDGLHLYEGLYRLLD
                     ADKVPGAPDLRDLNGDGSADLGLLVNCGLPHNVNYAHFLWNEAMGQLVFSGLFFAPLE
                     LEDTGGVVETEHVSNYPDIRRRYTFSRYGVPLLTGVVSRGALPPEESTLPIPEEGVHF
                     TFSSGAGAWSTDLTLFPDGTFSGKFHDRDMDITYLCQFQGAFSMLTPLDSRTWSLTLT
                     DLEVTTGRPEGMEWTEGGIRYVAAAPYGLDGGNEFCLYLPGTPAENISPRCRSWSGGY
                     YGLFEAGAVLSGYGLCDLRWGYGFFSFS"
     gene            1312..3261
                     /locus_tag="ACZPAQ_05160"
     CDS             1312..3261
                     /locus_tag="ACZPAQ_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903407.1"
                     /translation="MARSQCPSTPFSTPLSGSAQQAEARIRNLFQGPKRRPPVWLMVL
                     VLLLILSYGGLVACQSQEAPPLEGDCVQTGEHDAPSARLIQAILDQGLSALDLTSPPV
                     SQVLATLPGNGCTLAAVSLSDGSSHYILAIGVVQESNGAVGPVFLVQGQGCQPHAVPF
                     QQDGTSYLLYTANSLSQWLSLGESGLLAFDGTQFTWVWPVEGDVREEGSAARAAYDSY
                     WENALALLAPGGVDVFARTGYAVIDGDGPQWLPDHNEQFYTAPEDQLNIGIYYQTRVW
                     LEDFTRSGRNPWNAHNASASWQILSLAPTDGSYPGRNDMAEASYDLLARADADSDLYL
                     AARIRYSPEEGCISVVQGWSMGSYSEVEAAMQALPEEDTVPSGLNSREFYALVRQYLS
                     ARLGADQIFFSGWDQLSPPQEGDLLLTLVDYLGSAALADNTVGSVYTMEAMQFRAGSW
                     VPQEAPFTLVLSRSAGDGVFLSVAGEAISNPAAMSLDEVARQPFRDLYDPEIALFRDG
                     WPTPAGPGLQDTALFFSQAGVREELPGEGLVQNEGDMWVRYSWDGFSADYYHLASDGS
                     ETLRGVETTRTDVYTLRGIRVGDSRQQVLASYPALKSESTRNPSLNEMDCMWYCEDMD
                     NDQGLALLFFFKGEHVSRIVLTRLL"
BASE COUNT          511 a         1140 c         1010 g          642 t
ORIGIN      
        1 ctggaggggc tcgctcagga gggctgcaat ccctggcaga cggagggcct ccccgccgcc
       61 tggcagatcc gtgccctcac ccccgccgat ggccgctacc tgctgtcctc ccctgccgtc
      121 tccgaggaag ctgactatct gttgacagcc caggcagact cggacccgga gttgtgcctc
      181 actgcccgcc tgcgcctccg acacgggacc ggggaagtac ctcaggccgt agagtggaca
      241 ataggcacgc cacaagagct ggggctggac ggggaacctc cccgctgccc cctccccctc
      301 tcggatggcc gcactctcac cctggagttt gacgagtctc ccatcgacgg catggagagc
      361 acggtgctca tccgtcaggt gaaggtgtgg gacggccctg tcctgctcca gtccatcacc
      421 gccggctccg tcttggagga cgggctccac ctctatgaag gcctgtaccg cctcctggat
      481 gcggacaagg tccctggcgc tcccgacctg cgggacctga acggggacgg ctccgcggac
      541 ctgggcctgc tggtcaactg cggtcttccc cacaacgtga actatgccca cttcctctgg
      601 aacgaggcga tggggcagct ggtgttctcc ggcctgtttt ttgcccccct ggagctggag
      661 gacacaggcg gcgtggtgga gacggagcat gtctccaact atccggacat ccgccgtcgg
      721 tacactttct ccaggtacgg cgtccccctg ctcaccggcg tggtatcccg gggggctctt
      781 cctccggagg agagcacgct ccccatccca gaggagggcg tccatttcac cttttccagc
      841 ggggctgggg cttggagcac cgacctgacc ctgttccctg acggcacctt ctccgggaag
      901 ttccacgacc gcgatatgga tatcacctat ctgtgccagt tccagggggc cttctccatg
      961 ctgacccccc tagacagccg cacctggtcc ctcaccctca cggacctgga ggtgaccacc
     1021 ggccgcccgg aggggatgga gtggacggag ggcggcatcc gctatgtggc cgccgccccc
     1081 tatgggctgg atggtggaaa cgagttttgt ctctacctcc ccggcactcc tgcggagaat
     1141 atttccccgc gctgccgctc ctggagcggc ggctattacg ggctgtttga ggcgggcgct
     1201 gttctctctg ggtacggcct gtgtgatctg cggtggggat acggcttctt ctccttctcc
     1261 tgacttcccg cccctccccc tgctccatcc tcactgaaag gacgtgcttt tatggccaga
     1321 tcccagtgtc cctccacccc cttctccacg cccctctccg gctccgccca gcaggcggag
     1381 gcgcgcatcc gcaacctgtt ccagggcccc aagcggcgac cgccagtctg gctgatggtc
     1441 ctggtcctgc tccttatcct gtcctacggc gggctggtgg cctgccagag ccaggaggct
     1501 ccgccgctgg aaggggactg cgtccagaca ggggagcatg atgcccctag tgcccggctg
     1561 atacaggcca ttctggacca ggggctctcc gccctggatc tcacctctcc tccagtcagc
     1621 caggtcctgg ccacgctccc gggaaacggg tgcaccttgg ccgctgtctc tctctccgac
     1681 gggtcctccc actatatact ggccatcggc gtggtgcagg agtccaacgg ggctgtcggc
     1741 cctgtctttc tggtgcaggg ccagggatgc cagccccatg ccgttccctt ccagcaggac
     1801 gggacctcct atctcctgta caccgccaac agcctttccc agtggctctc tctgggagaa
     1861 tccggtcttc tcgctttcga cggcactcag ttcacctggg tctggccggt ggagggagac
     1921 gtccgggagg agggctccgc cgcccgggcg gcctatgact cctactggga gaacgctctg
     1981 gcgctccttg ctccaggcgg cgtggacgtg tttgcccgga cgggctacgc cgtcattgac
     2041 ggagacggcc cccagtggct ccccgatcac aatgagcagt tctatacggc ccctgaggac
     2101 cagctgaaca tcggcatcta ttaccagacc cgggtgtggc tggaggattt cacccgcagc
     2161 gggcgtaacc cctggaacgc ccacaatgcc tccgcctcct ggcagatcct ctccctcgcc
     2221 ccgacggacg ggtcctatcc agggcggaac gacatggcgg aggcctccta cgacctgctg
     2281 gcccgggcgg acgcagacag tgacctctac ttggccgccc gcatccggta cagccccgaa
     2341 gagggatgca tctctgtcgt ccagggctgg tccatgggct cttattcgga ggtagaggcc
     2401 gccatgcagg ctctgccgga ggaggacacc gtaccctccg gcctgaacag cagagaattt
     2461 tatgccctgg tccgtcagta tctgtctgcc cgtctggggg cggaccagat cttcttttcc
     2521 ggctgggacc agctttctcc tccccaggag ggggatctgc tcctgactct ggtggactat
     2581 ctcggctccg ccgccctggc ggacaacact gtgggctcgg tctataccat ggaggccatg
     2641 cagttccgtg ccggcagctg ggtccctcag gaggcgccct tcaccctcgt cctcagccga
     2701 agcgccgggg acggagtctt tctgtccgtg gcgggggagg ccatctccaa tcctgccgcc
     2761 atgtctctgg acgaggtcgc ccgccagccc ttccgggatc tgtacgatcc ggagatagcc
     2821 ctgttccggg acggatggcc cacccccgct gggcccgggc tccaggatac agctctgttt
     2881 ttctcccagg ccggcgtccg ggaggagctg cctggtgagg ggctggtcca gaacgagggg
     2941 gacatgtggg tccgatacag ctgggacggc ttttccgccg actattacca cctggcctcc
     3001 gacggctccg agaccctccg gggagtggag accacccgga ccgatgtgta caccctccga
     3061 ggcatccggg tgggagacag ccgccagcag gtcctggcct cctatccagc tctgaagtcc
     3121 gagagcacca ggaacccctc cctgaatgag atggactgca tgtggtactg tgaggacatg
     3181 gacaacgatc aggggctggc tcttctgttc ttttttaaag gggagcatgt cagccgcatc
     3241 gtcttgaccc ggctgctctg agccccctcc gcataccgaa aggagatgag accatgaaac
     3301 agc
//
LOCUS       DBMDSJ010000171         3297 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7269_length_3297_cov_1.553817, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000171 DBMDSJ010000000
VERSION     DBMDSJ010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7269_length_3297_cov_1.553817"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="ACZPAQ_05165"
     CDS             complement(<1..474)
                     /locus_tag="ACZPAQ_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR0903408.1"
                     /translation="MSIEKVRQYFQPLGREQDILEFETSSATVELAAQAAGVIPARIA
                     KTLSFLVDEGCVLIVTAGDAKIDNSKFKAMFHAKAKMLSPEQVYSFTGHAVGGVCPFA
                     NPDGVRTYLDVSLQRFETVFPAAGSSNSAIQLTCDELAEYSHSLGWIDVCKGWQET"
     gene            complement(480..872)
                     /locus_tag="ACZPAQ_05170"
     CDS             complement(480..872)
                     /locus_tag="ACZPAQ_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR0903409.1"
                     /translation="MSVEIGLKGRAEETVTDANTAQAACSGALPVYGTPFMTALMEKA
                     AWTSIAPRLAPGESTVGTAMNITHISASPVGIRVWAESEVTLVDGKRIELKVAAYDQT
                     GKIGEGTHQRFIVTDDRFLAKAARKLEG"
     gene            complement(952..2232)
                     /locus_tag="ACZPAQ_05175"
     CDS             complement(952..2232)
                     /locus_tag="ACZPAQ_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR0903410.1"
                     /translation="MDIHVVKPGDTLYSIALSHGVPMSRLLEDNQLPDPSRLVVGQTI
                     VIQYPERTYVVRSGDTLFSIAQANGLTVKTLLRNNPSLAGEDRIFPGQELVLAYRQEK
                     QGTLTVNGYAYPHIDRALLQRTLPYLSGLLPFSYEATALGELTPLDDVALVDAAKQMG
                     VVPIMNITNLTPDGMFSPELAHIILSDPAIQEKLAANVMEVIRARSYQGLDVDFESIY
                     AEDAQSYVSFISRLRELAAPMGIPVLVAVAPKSYANQPGLLYEGMDYAGLGRAADLIF
                     LMTYEWGYSYGPPMAISPIRSMRTVIEYALKEIPAEKLLLGIPNYGYDWQIPYSQSRK
                     AVSISNQYAVSLAARYYAAIRFDESVQAPWFRYVDERGQEHEVWFEDARSIRAKLSLV
                     PEYGLRGVGYWNLMRPFPQNWVILNALYHIREGL"
     gene            complement(2248..2988)
                     /locus_tag="ACZPAQ_05180"
     CDS             complement(2248..2988)
                     /locus_tag="ACZPAQ_05180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR0903411.1"
                     /translation="MPIIYLSPSTQEWNYYVNGGTEEEYMNLLADKMVPYLDASGIRY
                     DRNTPSMTAADSIAASNAGNFDLHLALHSNAAPEGQYGTSRGSIAFYYPGSIQGKWAA
                     DIIANNLRSIYPLPDKVRAVSTTTLGEVRKVRAPAVLLELAFHDNPDDAAWIKNNLDE
                     IARNLVLSVTEFFGLPFLTPIPARSGMVDVNWGSLNIRSRPSLDAPVLAQAPDGALLT
                     IINQWQDWYLVNYNGTIGYAKDSFVTLN"
BASE COUNT          634 a          910 c         1124 g          629 t
ORIGIN      
        1 tgtctcttgc cagcccttgc acacgtcgat ccatcccagg ctgtgggagt actccgccag
       61 ctcgtcacag gtgagctgga tcgcggagtt gctgctgccc gctgcgggga agacagtctc
      121 gaagcgctgg agggacacat ccaggtaggt acgcaccccg tccgggttgg caaaagggca
      181 gacgccgccc actgcatggc ctgtaaagga atatacctgc tcaggactga gcatctttgc
      241 cttggcgtgg aacatggctt tgaacttgga attgtcgatt ttggcgtccc ctgcggtcac
      301 gatcagcaca cagccctcgt ccaccaggaa ggagagggtc ttggcgatcc gggcggggat
      361 gacgccggcg gcttgggcgg ccagctccac cgtggcgctg gaggtctcaa attccaggat
      421 atcctgctca cggcctaaag gctggaaata ttgccggact ttttcaatgg acatgccgct
      481 cagccctcca gtttccgggc cgccttggcc agaaagcggt catccgtgac gataaagcgc
      541 tggtgggtgc cctcaccgat cttgcccgtc tggtcatagg cggccacctt cagctcgatg
      601 cgcttgccgt ccaccagggt gacctcgctc tccgcccaga cccggatgcc tacgggggag
      661 gcggagatgt gggtgatgtt catggcagtg cccaccgtgc tttcgcccgg cgccaggcgg
      721 ggggcgatgg aggtccaggc cgccttttcc atcagagcgg tcataaaggg cgtaccgtag
      781 acgggcagag cgccagagca ggcggcctga gcggtattcg cgtcggtcac cgtctcctcc
      841 gcgcggccct tcaggccaat ttcaacagac atgaaaatgt cctcctttgc acaaatgata
      901 cctcatttta ctacaaagga ggacaaaaat ccagcggcgg agagaaaaat gttacagccc
      961 ttcccgaatg tgatacaggg cgttgaggat cacccagttc tgggggaagg gacgcatcag
     1021 gttccagtac cccaccccac gcaggccgta ttccgggacc agactcagct tggccctgat
     1081 gctgcgggca tcctcgaacc agacctcgtg ctcctgtccc cgctcgtcca cgtaccggaa
     1141 ccagggggcc tggaccgact cgtcgaaccg gatggcagca tagtagcggg cggccaggga
     1201 gacggcgtac tggttggaga tggagaccgc ctttctggac tgggagtagg ggatctgcca
     1261 gtcatagcca taattcggga tgcccagcag gagcttctct gctgggatct ccttcagggc
     1321 gtactcgatc acggtgcgca tgctgcggat gggggagatg gccatagggg gaccgtagga
     1381 gtagccccac tcataggtca tgaggaatat caaatcggca gcccggccca gcccggcgta
     1441 gtccatcccc tcatagagca gacccggctg gttggcgtag gacttggggg ctacagccac
     1501 aaggaccggg atgcccatgg gcgcggcaag ctcccgcagg cgggagatga aggacacata
     1561 agactgggca tcctcggcgt agatggattc aaagtccacg tccagcccct gatagctgcg
     1621 ggcacggatg acctccatca cgttggcggc cagtttctcc tggatggcgg gatcggagag
     1681 gatgatatgg gccagttccg gcgagaacat gccgtccggt gtcaggttgg tgatgttcat
     1741 gatggggacc acccccatct gtttggccgc atccaccagg gccacgtcgt ccagcggggt
     1801 gagctccccc agggcggtgg cctcgtagga gaaggggagc aggccgctga gataggggag
     1861 ggtgcgctgg agcagggcgc ggtcgatgtg ggggtaggcg tatccgttga cggtgagggt
     1921 cccctgtttc tcctggcggt aggcgaggac cagctcctgc cctggaaaga tgcggtcctc
     1981 ccccgccaga gaggggttgt tgcggagcag ggtcttgaca gtcaggccgt tggcctgagc
     2041 gatggagaag agcgtatcgc cggagcggac cacataggtg cgctccgggt actggattac
     2101 gatggtctgt cccaccacca gccgggaggg gtcagggagt tgattgtcct ccagcagacg
     2161 ggacatgggc acgccgtggg aaagggcgat ggagtacagg gtatcgcccg gtttgacaac
     2221 atggatatcc atagaaacct ccttgcatca gttcagcgtc acgaagctgt ctttggcgta
     2281 gccaatggtg ccgttatagt tgaccagata ccagtcctgc cattggttga tgatggtgag
     2341 gagcgcaccg tccggggcct gggccagaac gggggcgtcc agggaggggc ggctgcggat
     2401 attgagggag ccccagttca cgtccaccat gccggagcgg gcggggatcg gagtcaggaa
     2461 gggcagtccg aagaactcag tgaccgagag gaccaggttg cgggcgatct catccaagtt
     2521 gtttttgatc caggcggcgt cgtccgggtt gtcgtggaag gccagctcca ggaggacggc
     2581 aggggcccgg accttccgca cttcgcccag cgttgtggtg gataccgccc ggaccttgtc
     2641 cggcaggggg tagatggagc gcagattgtt ggcgatgatg tcggcggccc acttgccctg
     2701 gatgctgccg gggtaataga aagcaatgga acctctggaa gtgccgtact gtccctcagg
     2761 ggcggcgttg gagtggaggg ccaggtggag gtcaaagttt cccgcgttgg aggcggcgat
     2821 ggagtcggcc gccgtcatac tgggggtgtt ccggtcatac cggatgccgg aggcgtccag
     2881 ataggggacc atcttgtccg ccaggagatt catgtattcc tcctcggtcc cgccgttgac
     2941 gtagtagttc cactcctgtg tggaggggga gagatagatg ataggcatac gattacctcc
     3001 cgattttttc ccattctatg aaaaaaaggg ggcggaggtg cggaactttt tccaggcgga
     3061 ggcgtctaat cggacagaga gacctgatct ttgccttgcg gaaacgatga aaaggaggag
     3121 cgataccatg aagcgaacca tgaaacgcgc gctttttccc ctgatgctgg ccctggtcct
     3181 gctgctgccc gcctgcggaa gcggcggcgg gaatggggcg ccggcggatc agactgcctc
     3241 cagcacgccg ggggctgccg gcggggagac cgcggaattt ggcgggatct atgaggt
//
LOCUS       DBMDSJ010000172         3290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7291_length_3290_cov_1.622139, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000172 DBMDSJ010000000
VERSION     DBMDSJ010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7291_length_3290_cov_1.622139"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(7..1329)
                     /locus_tag="ACZPAQ_05185"
     CDS             complement(7..1329)
                     /locus_tag="ACZPAQ_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903412.1"
                     /translation="METAALTRGSIPPQLVSLALPLIFGNILQQLYNTIDAMVIGRFA
                     GETAFAAIGVAGTVMNLFLFLLSGCCTGISVLFAQQYGSRDLAGFRQEGFLASVFGGL
                     FTLVLSLAALLLLRPLLTLMQTPEDVARLAADYLVVIFGGLLATFFYNLCAAALRSVG
                     DTRSALLALLAAMAANLALDLLFVARLGMGIAGAAWATVLAQLLSVALCLLYLARRYP
                     QLLFRREDRRLDRGLLRRTVSYGAVSALHQSSLYIGKLLVQGAVNSMGTPMISAYTAT
                     TRIEGFANSFGDSGAQAVSVFTAQNTGAGEERRVREGFRTSFLMMLALGAVMSVLLYA
                     AAPAAIALLAGETSSFTQTNGVAYLRMVGCFYILCFLGNTFVGYFRGRGQVTVPVYGT
                     IIQISARVILSYALIRPMGLSGVALATGLGWVLVVSFHSLMYWRTRNR"
     gene            complement(1514..2263)
                     /locus_tag="ACZPAQ_05190"
     CDS             complement(1514..2263)
                     /locus_tag="ACZPAQ_05190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0903413.1"
                     /translation="MTQELLSGLNGYDIPCRHTIQGGESMVCLITHGFGSSKDSPTAQ
                     LLAGELPRHGIGTLALDLPAHGDSPVDGDALSIRHCLDDLARAEARALELAPGARVVY
                     FSSSFGAYLNLIYLSTRPHRGTRSFLRSAAVEMPLLFHSPTPEQAEELARQGFLMLDQ
                     GYVRPLKLTPAFLADLDAHDLFQLYRSGEERVCMIHGDEDQTASPEAARRFAQTFGAR
                     LIQVPGGDHSLSGPGMPQLVVREALEFYLAP"
     gene            complement(2381..3046)
                     /locus_tag="ACZPAQ_05195"
     CDS             complement(2381..3046)
                     /locus_tag="ACZPAQ_05195"
                     /inference="COORDINATES: protein motif:HMM:NF020904.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HIR0903414.1"
                     /translation="MIPLEERNFVISACQKLQERGWFPGKEFVSSSITEQDIATFEQA
                     HQVKLPSLYRAFLTSFRLPQNIRNDNFLCSIIEDKGDLGPLWLAIDCPQSMNEVSKNI
                     EILQEIRDFCELPEDCFRNLIPIGDWGGGWGPLCIDLSIPEEEIDENDPLTWSLVWFD
                     HEEFDWNEEYLSEDGLLHGSKALPDLKKLLELYFYGALEKRFEQEEGIKPTYEWYQDT
                     LKR"
BASE COUNT          726 a          937 c         1047 g          580 t
ORIGIN      
        1 gagggatcag cggttccggg tgcgccaata catgagggag tggaagctca ccaccagcac
       61 ccagcccagg ccggtggcca gggccacgcc ggacaggccc atggggcgta tcagggcgta
      121 ggagaggatg acccgggcgg agatctggat gatagtgccg tagacgggga cggtcacctg
      181 tccccggccc cggaagtagc ccacgaaggt gtttcccagg aagcagagga tgtaaaagca
      241 ccccaccatc cgcaggtaag ccacaccatt ggtctgggtg aaggaggagg tctcccccgc
      301 cagcagggcg atggcggcgg gggcggcagc gtacagcagc acagacatca ctgcgcccag
      361 ggccagcatc atgagaaagc tggtacggaa cccctcccgc acccgccgct cctccccggc
      421 tccggtattc tgggcggtga agacagagac agcctgggcc ccgctgtccc cgaaggagtt
      481 ggcgaagccc tcgatgcggg tagtggcggt gtaggcggag atcatggggg tccccatgga
      541 gttcaccgcc ccctggacca gcagcttgcc gatgtagagg ctggactggt gcagggcgga
      601 caccgcccca tagctcaccg tccggcggag caggccccgg tccagccgcc ggtcctcccg
      661 gcggaacagg agctggggat agcgccgggc cagatagagc aggcacagcg ccacggagag
      721 cagctgggcc agcaccgtgg cccaggccgc cccggcgatg cccatgccca gccgggccac
      781 gaacagcaga tccagagcca gattggcggc catagccgcc agcagggcca gcagggcgga
      841 gcgggtgtct cccacagagc gcagggcggc ggcgcacagg ttgtaaaaga aggtggccag
      901 caggccgccg aagatgacca ccagatagtc cgcggccagc cgggccacgt cctccggggt
      961 ctgcatcagg gtcagcaggg gccgcagcag gagcagggcg gccaggctca gcaccagggt
     1021 gaacaggccg ccgaagacag aggccagaaa cccctcctgc cggaagccgg ccagatcccg
     1081 gctgccatac tgctgggcaa agaggacgga gatgcctgtg cagcagccgc tgaggagaaa
     1141 aaggaacagg ttcatcaccg tccccgccac cccgatggcg gcaaaggcgg tctccccggc
     1201 gaagcggccg atgaccatgg cgtctatggt gttgtacagc tgctggagga tgttcccaaa
     1261 aatcaggggg agggccagcg acaccagctg gggcgggatg gagccccggg tcagggcggc
     1321 ggtctccaca gcgtgtcacc tcacagtttc atcaggtgag atcaggatag cacagggaga
     1381 aacagttgta aaataattgt tttctcccta taatatataa aaaagttgta ttttggggtg
     1441 agacaggtga cagagctgga caccctgcgg gcggagctgg tccaggtcga gggcatcgag
     1501 ctgtgctgag ggctcagggg gccaggtaga actccagggc ctcccggacc accagctggg
     1561 gcatgccggg gccggacagg ctgtggtccc cgccgggcac ttggatgaga cgggcgccga
     1621 aggtctgggc aaagcgccgg gccgcctccg gcgaggcggt ctggtcctca tccccgtgga
     1681 tcatacagac ccgctcctcc ccggagcggt acagttgaaa gaggtcgtgg gcatccagat
     1741 ccgccaggaa ggcaggggtc agcttcaggg ggcggacgta gccctggtcc agcatgagga
     1801 agccctgccg cgccagctcc tccgcctgct ccggcgtggg ggaatggaaa agcaggggca
     1861 tctccaccgc cgcgctgcgc aggaaggacc gcgtcccccg gtgggggcgg gtggacaggt
     1921 agatcagatt caggtaggcc ccgaaactgg aggagaaata gaccacccgg gccccggggg
     1981 ccagctccag ggcccgggcc tctgcccggg ccaggtcgtc caggcagtgg cggatgctca
     2041 gagcgtcccc gtccaccggg ctgtccccgt gagcggggag gtccagtgcc agggtgccga
     2101 tgccgtgccg ggggagctcc ccggccagca gctgggcggt ggggctgtcc ttgctgctgc
     2161 cgaagccgtg ggtgatgagg cagaccatgg actcgccgcc ctggatggtg tgccggcagg
     2221 ggatgtcata tccgttgagt ccggagagaa gttcctgtgt cataagcgct cctttcaaag
     2281 ggcgagggcc cctttttctg ctgcactgcc aaaagctgat ttgaaaatac cacagcaaaa
     2341 tgaccaggcg ggcaaccgac ggtcaaactg gcagttgcag ttatcgtttc aaggtatcct
     2401 gataccattc gtaagtgggt ttgataccct cctcttgctc aaagcgtttt tccaatgctc
     2461 cgtaaaaata taactccagt aatttcttca aatccggcag agccttactt ccgtgaagca
     2521 gtccatcttc actcaggtat tcttcattcc aatcaaattc ctcgtggtca aaccagacta
     2581 aagaccaggt gagtgggtcg ttttcatcaa tctcctcctc tggtatggac aaatcaatgc
     2641 acagcggccc ccaaccacct ccccaatcgc caatgggaat caaatttcga aagcagtcct
     2701 ccggtagttc acaaaaatca cggatctctt gcaaaatttc tatgtttttt gacacttcgt
     2761 tcatagattg tgggcagtcg attgccaacc agagtgggcc aaggtcgcct ttgtcttcaa
     2821 taatagagca tagaaaattg tcattgcgaa tgttttgagg aagccggaat gaagtcagaa
     2881 aagccctgta gagagagggc agcttgacct gatgcgcttg ctcaaatgta gcaatgtcct
     2941 gttcagtaat ggaagatgac acaaattcct tgccagggaa ccaaccccgc tcttgtaatt
     3001 tttgacatgc gcttatcaca aaatttcttt cttccaaagg tatcatggtg tcccttgctc
     3061 cttccttggg agtccaaaca acctccaatt tgtcttttta cttactttac catattttta
     3121 tgtcttaatc aatgtgtagt gaaatggcca gagttggaac caatcagtca aagataaaaa
     3181 tggagaggcg ggacccgccg ttgtcagcca ggacagactc tgctagaata ggagagagac
     3241 cgaccggggc tctctggaga ggaggaatgg aaatggaggg atgggacctg
//
LOCUS       DBMDSJ010000173         3290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7292_length_3290_cov_1.335215, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000173 DBMDSJ010000000
VERSION     DBMDSJ010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7292_length_3290_cov_1.335215"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="ACZPAQ_05200"
     CDS             complement(<1..825)
                     /locus_tag="ACZPAQ_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR0903415.1"
                     /translation="MITVTDLGLQYGGQPLFSHVDLQFTRGNCYGIIGANGAGKSTFL
                     KILSGDLESTSGTVSILPNVRMSVLKQDQNAYDAYNVMDTVIMGNQRLYEVGREKEAI
                     YAKEEMTDEDGMRACELEEEYAELGGWEAESDASRILQGLGIGTEYHTSDMATLDARL
                     KVKVLLAQALFGNPDILMMDEPTNNLDIDAINWLEDFLLDFEGTVIVVSHDRHFLNTI
                     CTHIVDIDYNKIKMYVGNYDFWYGSSQLVQQLIKNQNKKNEEKIKELQDFISRFSAN"
     gene            complement(1107..2408)
                     /locus_tag="ACZPAQ_05205"
     CDS             complement(1107..2408)
                     /locus_tag="ACZPAQ_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024948.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR0903416.1"
                     /translation="MNQSRSRLVDLAGLLSNVTALHTLFSIFAFRQTGRSDPAGLLIW
                     CVCLSLTCGGLALFLRRPRTQRSVLLLALAALALQGAVVWLWGVRYPSLLSWLCLLAM
                     WGFSYFRCYELLIRPTPAEQLMVSFETASLVLLAAALCTAAGVLPLDTALAPAAGTLL
                     ALAALAGQRASNSRPGGQAAGSLRGRLLLLLLLAALGGGAALCVLLFTGTAAQALTAL
                     TGWLRELASGALALLDRFFRWLFSLFPAPETGSLNLPDPISAAGGPAGEAVQSSDAVL
                     YVLAGLVLLAGVGAILWALRRGGSRTRSHPPVRRGRVVDRRSPRDTLAMLWSRLADRV
                     RFQLTYLARRNTPAGVLVWLDRRLKRRRKGRRPGETCRAFLDRAAAELPGCAQELGEL
                     ARCLDQLYFGGGAPAFSPARASELRRALRLASRPPERPARH"
     gene            complement(2405..>3290)
                     /locus_tag="ACZPAQ_05210"
     CDS             complement(2405..>3290)
                     /locus_tag="ACZPAQ_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR0903417.1"
                     /translation="ALRQSFSFVGSWQTIQVESTWTAQRRGLYRVHRLVARSGDGFGL
                     VQGEQPLPAGQIPAIAVYPRPVEVDLSLFLRPQWDCAAGRQGWMEDNTVLRGCREYQP
                     GDSWKHINWRMAAREQGTPVNLYQTIQPRGLRFLLDGESFCGLSQDWRELEGALEVLA
                     SVLSGLSGAGVDCSLSLPHSRLFGPMTVSSGEGGGDDLLFYLAGYRCLAQRVPEESRS
                     PQGPAFLPSRFAADAAPQSGTAYLITCSGSRLPAALMERLDPGQLWVLCAQDWEVPAR
                     AGFRSLSLDSLRKGGSRP"
BASE COUNT          597 a         1028 c         1190 g          475 t
ORIGIN      
        1 gttggcggag aagcgggaga tgaagtcctg gagctccttg atcttctcct cgttcttctt
       61 gttctgattc ttgatgagct gctgcaccag ctgagaggac ccataccaga agtcgtagtt
      121 gcccacgtac atcttgatct tgttgtagtc gatatccacg atgtgggtgc agatggtatt
      181 gaggaaatgg cggtcgtggc tgaccacgat gacggtgccc tcaaagtcca gcaggaagtc
      241 ctccagccag ttgatggcgt cgatgtccag gttgttggtg ggctcgtcca tcatcagaat
      301 gtccggattg ccgaacaggg cctgggccag cagcactttc accttcaaac gggcatccag
      361 ggtggccatg tcgctggtgt gatactccgt gccgatgccc agcccctgga gaatgcggct
      421 ggcgtcactc tccgcctccc agccgcccag ttcggcgtac tcctcctcca gctcacaggc
      481 ccgcattccg tcctcgtcgg tcatctcctc cttggcgtag atggcctctt tctccctgcc
      541 cacctcatac aggcgctggt tgcccatgat gacggtgtcc atgacgttgt aggcatcata
      601 ggcgttctgg tcctgcttca gtaccgacat gcgcacgttg ggcaggatgg acacggtgcc
      661 gctggtggat tccaggtccc cggacaggat cttcaaaaag gtggacttgc cagccccgtt
      721 ggcgccgatg atgccgtagc agttgccccg ggtgaattgc aggtccacgt gagaaaacag
      781 gggctgtccg ccgtactgca gccccagatc ggtgacggtg atcatagtcg tctcgctcct
      841 tacactccat ggtaaaaaca gtttgcgtcc gctcagtgca caaagggcgg cacgcccgcg
      901 gtctgcccgc cccgaaacgc cgtgcggtcc tctgcacact gaggttacta tcttatcaca
      961 aacaggagga aaaggataga gtattttccg gaaaattggg ggcatttttt cgtttacagc
     1021 tgtttttttg accaacagga cagcggaaag ggagaaaaag cgccccagga ctctccactg
     1081 ggagatctcg gggcgcggag ggggagtcag tgccgtgcgg gcctctccgg cggacgggag
     1141 gccaggcgca gggctcggcg gagctctgag gctcgggctg gggagaaggc gggggctccg
     1201 ccgccgaagt agagctggtc cagacagcgg gccagctccc ccagctcttg ggcacagccg
     1261 ggcagctccg ccgccgcccg gtccaggaag gcgcggcagg tctcccccgg ccggcggccc
     1321 ttacggcggc gcttcaggcg gcggtccagc cacaccagca cgccggcggg ggtattccgc
     1381 cgggccaggt aggtgagctg gaagcggacg cggtccgcca gccgggacca cagcattgcc
     1441 agggtgtccc gtgggctgcg gcggtccacg acgcggcccc tgcggaccgg agggtgggac
     1501 cgggtgcggc ttccgccccg gcggagggcc caaaggatcg cgccgacgcc ggccagcagg
     1561 accaggccgg ccagcacata gaggaccgca tcgctgctct ggacggcctc gccggccggg
     1621 ccgcctgcgg cggagatggg atcgggcagg tttagggagc cggtctccgg ggcagggaag
     1681 agggagaaga gccagcggaa gaaccggtcc agaagggcca gcgccccaga ggccagctca
     1741 cgcagccagc cggtgagggc ggtgagggcc tgggcggcgg tgccggtgaa cagcagcacg
     1801 cacagggctg cgcccccgcc cagggcggcc agcagaagga gaaggagcag acgcccccgg
     1861 agggagccgg cggcctgtcc gccgggacgg ctgttggagg ctctctgtcc ggccagggct
     1921 gccagggcca gcagagtgcc ggcggcgggg gccagggctg tgtccagggg gaggacaccg
     1981 gcggcggtgc acagggcggc ggccagcagg accagggagg cggtctcgaa ggagaccatc
     2041 agctgctccg cgggcgtggg gcggatcagc agctcatagc agcggaagta ggagaagccc
     2101 cacatggcaa gcaggcacag ccaggacagc agggagggat agcgcacccc ccacagccag
     2161 acgacggccc cctggagggc cagagcggcc agcgccagaa gcaggacgga gcgctgggtc
     2221 cgggggcgcc ggagaaacag ggccagaccc ccacaggtca gggagaggca gacgcaccag
     2281 atgagcaggc cggccggatc agaccggccg gtctgacgga aggcgaagat ggagaacagg
     2341 gtgtgcaggg cagtcacatt ggacagcagg ccggccaaat cgacaaggcg ggaacggctc
     2401 tggttcacgg gcggctcccc cctttccgca gggagtccaa ggagagggac cggaacccgg
     2461 cccgggcggg gacctcccag tcctgggcgc acagcaccca cagctgccct gggtccagcc
     2521 gctccatcag ggcggcgggg aggcggctgc cggagcaggt gatcagatag gcggtgccgg
     2581 actggggggc tgcgtcggcg gcgaagcggg aggggagaaa ggccggcccc tggggggagc
     2641 gactctcctc tgggacccgc tgggccaggc agcggtagcc cgccagatag aagagcaggt
     2701 cgtccccgcc gccctccccg gaggagacgg tcatgggacc gaagaggcgg gagtggggga
     2761 gggagaggga gcagtccacc cctgcgccag acaggccgga aaggacggag gccagtacct
     2821 ccagggcccc ctccagctcc ctccagtcct gggagagacc gcagaaggat tccccatcca
     2881 gcagaaagcg cagccctctg ggctggatgg tctggtacag gttcaccggc gtgccctgct
     2941 cccgggcggc catgcgccag ttgatatgct tccagctgtc tccgggctgg tactcccggc
     3001 agccccggag cacggtgttg tcctccatcc acccctgacg gccggcggcg cagtcccact
     3061 gggggcggag aaagagggag aggtccacct ccaccggccg gggatagacg gcgatggccg
     3121 ggatctgccc ggcggggagg ggctgttccc cctggaccag cccgaagccg tccccggagc
     3181 gggccaccag tcggtggacc cggtacagtc cccgccgctg ggcagtccag gtgctctcaa
     3241 cctggatggt ctgccagctc cccacaaagg agaaggactg gcgcagggcg
//
LOCUS       DBMDSJ010000174         3263 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7349_length_3263_cov_2.018027, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000174 DBMDSJ010000000
VERSION     DBMDSJ010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7349_length_3263_cov_2.018027"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="ACZPAQ_05215"
     CDS             <1..282
                     /locus_tag="ACZPAQ_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0903418.1"
                     /translation="LFSRKARANWEMLDLMVTQPHIPTEALARLPMPALVVAGERDMI
                     REGHTRAIAAAIPDSRLAILPGDHFVARRNWQTFNPLVLDFLLDGAQTP"
     gene            305..1066
                     /locus_tag="ACZPAQ_05220"
     CDS             305..1066
                     /locus_tag="ACZPAQ_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0903419.1"
                     /translation="MDDWFTLERIDEDTDILSEYRHWEETHCYLLRGRERSLLIDTGL
                     GICDIRQAVDRLTDRPVAAVATHIHWDHIGGHRYFPEFYAHEAELNWLSGGFPLSVEA
                     VRAMVADRCCLPEDFDVSRYTLFQGHPARVLRDGDRIELGGRTLEVLHTPGHSPGHMC
                     FWEAERGYLFSGDLVYRGTLFACYPSTNPEEYLSSLERTACLPMERLLPAHHALDIGP
                     ELLGRVRDAFRGLKEQGKLRHGGGTFDYGDWAVRL"
     gene            1113..1946
                     /locus_tag="ACZPAQ_05225"
     CDS             1113..1946
                     /locus_tag="ACZPAQ_05225"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HIR0903420.1"
                     /translation="MQQNKNILIINDMPGYGKVALAAMLPILSHMGHNIYNLPTALVS
                     NTLDYGKFTILDTTDYMKKSIAVWKELGFSFDCITTGFLASAEQVDIIRAFIESQRKE
                     DLLVMTDPIMGDEGKLYNGVTEETVENMRRLIGVADVIVPNLTEAEFLTERYRGAEAL
                     DRHQAREVVDALLACGPRSVVITSGLEAETGRHVVWGYCHRQGRYFTLPFDFIKVHFP
                     GTGDVFSAVLVGELLAGTGLEQAARKAMDTVERLVCLDRNEVEKNKGIRIERFLKELD
                     L"
     gene            1982..2395
                     /locus_tag="ACZPAQ_05230"
     CDS             1982..2395
                     /locus_tag="ACZPAQ_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR0903421.1"
                     /translation="MWAVFALLSAVFAALTGILAKLGMEGINSNLATAIRTVVVLVMA
                     WAIVFLTGKQHEIGDISTRSWVFLLLSGLATGLSWLCYFHALQIGQASRVIPIDKFSV
                     VISMVLAFVVLREAVTVQTVIGGLLITAGTLVLIL"
     gene            2427..>3263
                     /locus_tag="ACZPAQ_05235"
     CDS             2427..>3263
                     /locus_tag="ACZPAQ_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR0903422.1"
                     /translation="MAFALTEEQSRAVHDRGGGLLVSAAAGSGKTRVLVERLLDQVTG
                     EGADIDDFLVITYTRAAAAELRARIAQELSDRLAERPNDRHLRRQTTLVYKAQISTVH
                     AFCAALLRESGHQIGLDPDFRLCDEGEGAVLMARVLGETLDRQYEDLDPEGDFAALVD
                     TMAAGRDDSRLEQIVLDVFGRIQSHPDPAGWLADQSRLWELDGVTDAGDTPWGALLLA
                     DARRQGERCLELLLRALELTGRDSVLSQNYGPSLSQSIQGVRALLAADSWDGVYRALP
                     VEF"
BASE COUNT          574 a          958 c         1155 g          576 t
ORIGIN      
        1 ctgttcagcc ggaaggccag ggcgaattgg gagatgctgg acctgatggt gacccagccc
       61 cacatcccca cggaggcgct tgcccggctc cctatgcccg ccttggtggt ggcaggggag
      121 cgggacatga tccgggaggg ccatacccgg gccatcgccg ccgccatccc ggacagccgg
      181 ctggccatcc tgcccgggga ccacttcgtg gctcggcgga actggcagac cttcaatccc
      241 ctcgtgctgg atttcctgct ggacggcgca cagaccccgt aggaattgga aaggaaagac
      301 agagatggat gattggttca cgctggagcg gatcgatgag gatacggaca tcctcagtga
      361 gtaccgccac tgggaggaga ctcactgcta cctgctgcgg ggaagggagc gcagcctgct
      421 catcgatacc gggctgggga tctgcgacat ccggcaggcg gtggaccgtc tgacggatcg
      481 gccggtggcg gcggtagcca cccacatcca ctgggaccac atcgggggcc accggtactt
      541 cccggagttt tacgcccacg aggcagagct gaattggctg tcgggcgggt tcccgctgtc
      601 cgtggaggcc gtccgggcca tggtggcgga ccgctgttgc ctgccggaag attttgatgt
      661 gagccggtac accctgttcc aggggcatcc cgcccgcgtg ctgcgggacg gagaccgcat
      721 cgagctgggc gggcggacct tggaggtgct ccacacgccg ggccactctc ctggccacat
      781 gtgtttttgg gaggcggagc ggggctatct tttttccgga gatctagtct atcgaggcac
      841 cctgttcgcc tgctacccct ctaccaaccc ggaggaatac ctgagttccc tggagcggac
      901 cgcctgcctg ccgatggagc gcctgctccc tgcccaccac gccctggaca tcgggccgga
      961 gctcctgggc cgtgtgcggg acgctttccg cgggctgaaa gagcagggaa agctccgcca
     1021 cggcggcggc acctttgatt acggggactg ggcggtccgg ctgtgacagg aaaaccggaa
     1081 aagcagaacg atgaaaaaag aaggaatgga ccatgcagca gaacaagaat attttgatca
     1141 tcaacgacat gccgggatat ggaaaggtcg ctttggccgc catgctgccg attttgtccc
     1201 acatggggca taatatctat aacctcccca ccgccttggt gtccaacacc ctggattatg
     1261 ggaagttcac catcttggat accaccgact atatgaaaaa gtccattgcc gtgtggaagg
     1321 agctgggctt ctcctttgac tgcatcacca cgggctttct ggcctcggcg gagcaggtgg
     1381 acattatccg cgccttcatc gagagccaga ggaaggagga cctgttggtg atgaccgacc
     1441 ccatcatggg ggatgaaggg aagctctata acggcgtcac cgaggagacg gtggagaaca
     1501 tgcgccgcct catcggggtg gcggacgtga tcgtccccaa tttgacggag gcggagttcc
     1561 tgacagagcg gtaccggggg gcggaggccc tcgaccggca tcaggcccgg gaggtggtgg
     1621 acgccctgct ggcctgcggg cccagatcgg tggtcatcac cagcgggctg gaggcggaga
     1681 ccggccgcca cgtggtgtgg ggctactgtc accggcaggg gcggtacttc accctgccct
     1741 tcgacttcat caaggtccac tttcccggca ctggcgacgt gttctctgcc gtcctggtag
     1801 gagagctgct ggccgggacc ggcctggagc aggcggcccg gaaggccatg gatacggtgg
     1861 agcgcctggt ctgtctggac cgcaacgagg tggagaagaa caagggtatc cgcatcgagc
     1921 ggtttttgaa ggaattggat ctctgagcct ccagagggcg gaagaaagga agaaaaggga
     1981 tatgtgggca gtttttgcgc tcctgtctgc ggtgttcgct gcgctgaccg ggatattggc
     2041 caagctgggg atggagggca tcaactccaa cctggccacc gccatccgca ccgtggtggt
     2101 gctggtgatg gcctgggcta ttgtgttcct gacagggaag cagcatgaga tcggggatat
     2161 ttccacccgg agctgggtgt tcctcctcct gtccggcctg gccaccggcc tgtcctggct
     2221 gtgctatttc cacgcccttc agatcggcca ggccagccgg gtcatcccca tcgacaagtt
     2281 cagcgtggtc atctcaatgg tgctggcctt tgtggtgctg agggaggcgg tgaccgtcca
     2341 gaccgtcata ggaggcctgc tcatcacggc agggacgctg gtgttgatcc tgtgaatgga
     2401 gcggacagag agaggaggac gcgcagatgg cctttgcctt gacagaggaa cagagcagag
     2461 cagtccatga ccgggggggc ggactgctgg tgtcggctgc ggccggctcg ggcaagaccc
     2521 gggtgctggt ggagcggctg ctggaccagg tgaccgggga gggagcggat atcgacgact
     2581 tcctggtcat tacatatacc cgggcggctg cggcagagct gcgggcccgg attgctcagg
     2641 agctgtccga ccggctggcg gagcggccca acgaccggca cctgcggcgg cagaccacgt
     2701 tggtctacaa ggcccagatc tccacggtgc acgccttctg tgccgctctg ctgcgggaga
     2761 gcggccacca gatcgggttg gacccggact ttcggctgtg cgacgagggg gagggcgcgg
     2821 tcctgatggc ccgggtgctg ggggagaccc tggaccgcca gtacgaggat ctggacccgg
     2881 agggggactt tgccgccctg gtggacacca tggccgccgg gcgggacgac agccgcctgg
     2941 agcagatcgt gctggacgtg ttcggccgca tccagagcca cccggacccg gcgggctggc
     3001 tggcagacca gagccgcctg tgggagctgg atggggtcac cgacgcgggg gacaccccct
     3061 ggggggcgct gctgctggcc gacgccaggc ggcaggggga gcgctgcctg gagctgctgc
     3121 tccgggcgct ggaactcacc ggccgggaca gcgtgctcag ccagaattac ggccccagtc
     3181 tctcccagtc catccagggg gtgcgggccc tgctggcggc ggacagctgg gacggcgtgt
     3241 accgggccct gccagtggag ttc
//
LOCUS       DBMDSJ010000175         3261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7356_length_3261_cov_1.592405, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000175 DBMDSJ010000000
VERSION     DBMDSJ010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7356_length_3261_cov_1.592405"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            170..997
                     /locus_tag="ACZPAQ_05240"
     CDS             170..997
                     /locus_tag="ACZPAQ_05240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR0903423.1"
                     /translation="MCDIIEVTDLDAPELDVYARLTQAQLRSRREPEKGIFIAESPKV
                     IGHALDAGCVPLSLLMERRQLEGQGRDLVERCGQVPVYTGDRAVLAGLTGYELTRGIL
                     CAMRRPRLPSVEELCARASRVAVLEGIVDPTNVGAIFRSAAALHVDAVLVTPTCCDPL
                     SRRAVRVSMGTLFQIPWTFLGTDASQWPQPGLERLKELGFQTAAMALDSTALSIEDPR
                     LRAAEKLAILLGTEGDGLAPRTIAQCDFTVCIPMARGVDSLNVAAASAVAFWELRAR"
     gene            complement(1297..1986)
                     /locus_tag="ACZPAQ_05245"
     CDS             complement(1297..1986)
                     /locus_tag="ACZPAQ_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0903424.1"
                     /translation="MSAPSAVQTPSRPKMDTKTMVSIAMLTGIAYIVMLLSKLMPSVM
                     FLDFDFKDVVVCIGGFTFGPFAAAIISILVAFIEMLTISHTGPIGFIMNVLATCAFSC
                     TACFIYKKVHSKKGAVLGLACGVACLVAVMLLWNYLITPLYMTGYSRADVAGLLPTLF
                     LPFNLAKGGMNMAATLLLYPPVVSAMRRAGVVPSSQSTQEKRINAGFVLFALSLLATF
                     VVFALVLAGVI"
     regulatory      complement(2100..2226)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2418..3137
                     /locus_tag="ACZPAQ_05250"
     CDS             2418..3137
                     /locus_tag="ACZPAQ_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR0903425.1"
                     /translation="MTGIGTLVNVCAIILGGFLGLLFKGGLKQHYQDAVLRCLGLCTL
                     FIGAAGGLSGLLSVSGTRLESGGSLGMILSLVLGTLVGERLNLEGRLGQLGEWLKARA
                     SRGEDSRFVEGFVTASLVVCVGAMAIVGSLQDALDHDPSTLFTKAILDFMIIMVFSST
                     YGRGAIFSALPVGVLQGSVTLFAGLLAPLLSGTVIDSLSFIGSLLIFCVGVNLAFGDR
                     FRVANMLPALIFGGIWASLMG"
BASE COUNT          581 a          976 c         1015 g          689 t
ORIGIN      
        1 tttaaaaaaa ataacatttg tcatcaattt tcttcctgaa aatgcgtttt tttccgctgc
       61 ttcaaaaaat acagcagaag gggacggagc agctctcttt ctgctggctt cttgcggatt
      121 ccgttcctgt tctgtataat taagatatat tgagttttgg ggagatccta tgtgtgatat
      181 cattgaggtg acagacctgg acgcgccgga gctggacgtg tacgcccggc tgacacaggc
      241 tcagctgcgc agccggcggg agccggaaaa aggtattttt attgcggaaa gccctaaggt
      301 gatcggacat gccctggacg cgggctgcgt ccccctctct ctgctgatgg agcgcaggca
      361 gctggaggga cagggccggg acctcgtaga gcggtgcggc caggtgccgg tctacaccgg
      421 ggaccgtgcg gttctggccg ggctgaccgg ctatgagctg acccggggca tcctgtgcgc
      481 catgcgccgg cccaggctcc cctctgtgga ggagctgtgc gcccgggcca gccgggtggc
      541 ggtgctggag ggcatcgtgg accccaccaa tgtgggggct atcttccgct ccgccgctgc
      601 cctgcatgtg gacgccgtgc tggtgacccc cacctgctgt gaccccctca gccgccgggc
      661 ggtgcgggtc agcatgggca ccctgttcca gatcccctgg acttttctgg gcaccgacgc
      721 atcccagtgg ccccagccgg ggctggagag gctgaaggag ctgggcttcc agactgcggc
      781 catggccctg gacagcaccg ccctcagcat cgaggacccc cggctccgcg cggcggagaa
      841 gctggccatc ctcctgggga cagaggggga cggcctggcc ccccggacca tcgcccagtg
      901 cgacttcacc gtgtgcatcc ccatggcccg gggggtggac tccctgaacg tggcggcggc
      961 cagtgctgtt gccttctggg agctccgggc ccggtagcgt ccccattgta tggaaaaacg
     1021 gcggaccgtc tggcggtccg ccgttttccg gcttgtgaaa aaagcctcgc agagttcgcg
     1081 cccgcaggcg cgaatccagt tttgatctgt tttccccggc gacacgtgcg tcggggaaaa
     1141 cacttctcag gctgcaagtg tggggtttgc ccgccgcagg cggtcaaacc ctgcgcgcgg
     1201 cagactgctg ttttctcgca aacgtggcac agccactttt gcgacagtct caaaaacggc
     1261 ggaccgtctg gcggtccgcc gtttttcttg tttgattcag atcacgcccg ccagcaccag
     1321 ggcaaagacc acgaaggtgg ccagcaggga cagggcaaac agcacgaatc cggcgttgat
     1381 ccgcttctcc tgggtgctct gggaggaggg caccactccg gctctccgca tggcggacac
     1441 caccgggggg tagagcagca gggtggccgc catgttcatc ccgcccttgg ccagattgaa
     1501 ggggaggaac agggtgggca gcagcccggc cacgtcggcc cgggaatagc cggtcatata
     1561 caggggggtg atgaggtagt tccacagcag catcaccgcg accaggcacg ccacgccgca
     1621 ggccaggccc aggaccgcgc ccttcttgga atggaccttc ttgtagatga agcaggcggt
     1681 gcaggagaag gcgcaggtgg ccaggacatt catgataaag ccaatggggc cggtgtggct
     1741 gatggtgagc atctcgatga aggccaccag gatagagatg atggcggcgg cgaaggggcc
     1801 gaaggtgaag ccgccgatgc agaccaccac gtccttgaag tcgaagtcca aaaacatgac
     1861 gctgggcatc agcttggaca ggagcatcac gatgtaggcg atgccggtga gcatggcgat
     1921 gctcaccatg gtcttggtgt ccatcttggg acgggagggg gtctggactg cggaaggtgc
     1981 ggacataaaa aacactcctt tttgttttgg tacacctgaa agcccatgtc ttccaggtgt
     2041 aaaacaataa aggaggtgcg caggaccgcg cgcctgacgg cgcacgacat tgttttgaca
     2101 cacatcttct tccatccgga ctttaaccgt tggtcccgga gtttcaccgg gtcagcgggc
     2161 gcattgctgc gcccggtcgc ggactgtacc gccagtgggg gatctcaccc cgccctgaag
     2221 acattcgatt cagttgtccg aactcattat agtgcatccc cgcgccaaat gcaacaaaaa
     2281 ttttgttccc ccctcccaca gcccctgcgg ttccgcgcct ccttccatat gccgcttcct
     2341 tttttcgtcg aaatctgata tagtagtcag gtcacagccc cggccctgcc ggtctggggc
     2401 cgcatgggag ggatcttatg accggcatcg gtacgctcgt caatgtgtgc gccattattt
     2461 taggcggatt cctgggcctg ctttttaaag gcggcctgaa gcagcactac caggatgcgg
     2521 tcctgcgatg tctgggcctg tgcaccctgt tcatcggcgc ggccggcggt ttgtccggcc
     2581 tgctgtccgt ctccggcacc cgcctggagt ccggcggctc cctggggatg atcctctctc
     2641 tggtgctggg caccctggtg ggggagcgct tgaacttgga gggccggctg gggcagttgg
     2701 gcgagtggct caaggccagg gcctcccgcg gggaggacag ccgctttgtg gagggcttcg
     2761 tcactgcctc cctggtggtg tgtgtaggcg ccatggctat cgtgggttcc cttcaggacg
     2821 cactggacca cgatccctcc acccttttca caaaggccat cctggacttc atgattatca
     2881 tggtattctc ctccacctac ggcagggggg ccatcttttc tgcccttccc gtaggggtcc
     2941 ttcaggggag cgtcaccctc tttgccggcc ttctcgcacc cctgctcagc ggcaccgtta
     3001 tcgacagtct ctcctttatc ggctccctgc tcatcttctg cgtgggcgtc aacctggctt
     3061 tcggggatcg gttccgcgtg gccaatatgc tccccgcgct gattttcggc ggcatctggg
     3121 cttccctcat gggatgattt aaaaaatggc atgccggcag actgacccaa acacttgttc
     3181 agtaacatga gtcattcaaa tcgctccaat cttgtactga atgggctgaa tcgctcttat
     3241 catgtcccat acgaatgact t
//
LOCUS       DBMDSJ010000176         3254 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7376_length_3254_cov_1.794481, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000176 DBMDSJ010000000
VERSION     DBMDSJ010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7376_length_3254_cov_1.794481"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            266..1207
                     /locus_tag="ACZPAQ_05255"
     CDS             266..1207
                     /locus_tag="ACZPAQ_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0903426.1"
                     /translation="MDLFQLKCFVNVVDQRSFTKAAFEVSSSQSALSKQISKLEDELN
                     VRLFDRSRRVVTLTPAGREFEPHARKLLADYDEMMASIKRFSNSGHLHIGSVDHMGRV
                     GLTTPISTFLKQYPDGGVTIDIERGTSLGVMDQLVAGKLDMAFIAHIISPFSKASNID
                     AYQLDHYRLYTLVVDNYHAIVSRRHRFAGLDVVTWQDLAEEKLVILDKSYGLNAIIRE
                     SFRQCGLRPNIAFECDQVDAILGMVEGNFGISILSKRIAATQYDVEAIPIDPPISRNT
                     VLVVPKEVEARQRLAGGFVRHIVDYYRDNTPGPCGEP"
     gene            1370..2065
                     /gene="vanR"
                     /locus_tag="ACZPAQ_05260"
     CDS             1370..2065
                     /gene="vanR"
                     /locus_tag="ACZPAQ_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HIR0903427.1"
                     /translation="METNILIVDDEAAIADLVEVYLKNEGYTVHKFGRAGDALACVAS
                     TPLDLAILDVMLPDMDGFTLCQKIREEHLFPILMLTARVEDMDKIMGLTLGADDYLTK
                     PFNPLELVARVKTQLRRYTRYNQGGQPGGEVQEHDFRGLQISRATHKCVLFGEELPLT
                     PLEFSILWHLCQHRGQVVSSEELFESVWGEKYMDSNNTVMSHIARLREKMHEPSRKPK
                     FIKTVWGVGYTIE"
     gene            2058..3212
                     /locus_tag="ACZPAQ_05265"
     CDS             2058..3212
                     /locus_tag="ACZPAQ_05265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903428.1"
                     /translation="MSKKRKPRLAQRLQTDRGLYRALQRWGYYLLALMAWVTVVLAVS
                     ALGVLVCSRLTWYETDPLYHLLLWVRDYYFFFCIAVVLLGWVVISWHFISRPVRQLDA
                     LIGASAQLTRPTEEPIRLPASMKSVEDQLNLAREEALRAQRAAREAEQRKNDLVVYLA
                     HDLKTPLTSVIGYLTLLRDEPQLSPELRARYTGIALDKAERLEELINEFFEITRFNLS
                     HLELEKQPVDLCRMLQQVVSEFAPMLGERDLTCALELPPSLEYDCDPDKLARVFDNLL
                     RNACHYSTPGSVVRISGGEEGDSIVLRFLNSGRTIPPEKLARIFDQFFRLDSSRATRT
                     GGAGLGLAIAKEILTLHGGTITAWSREDQVCFQVTLPRGGRREEGPAREP"
BASE COUNT          601 a         1052 c          979 g          622 t
ORIGIN      
        1 tttcttctgt tgtttggcgt cattttaacc aaaattcggg acaaaaagca atctcacttt
       61 ggaaagtcat atttccgaaa cggaatcgga ttctattttc aaggcatatt ccattccaaa
      121 acagcatatc attctgtttt ggaattttta ttttccaatt tggtatcata ctgcctggga
      181 ttttgggaaa ctctcgttgt ctttctccgc cccccatggt atcatagggc atatcacggc
      241 aatctgcaca agggatggat gtcagatgga cctgtttcag ttgaaatgct ttgtcaatgt
      301 ggtggatcag cggagcttta caaaggccgc ctttgaggtc tccagctccc agtccgccct
      361 gtccaagcag atcagcaagc tggaggatga gctgaacgtc cgtctgtttg accgcagccg
      421 ccgggtggtg acgctgacgc ccgcaggccg ggagttcgag ccccacgccc gaaagctgct
      481 ggccgactat gacgagatga tggcctccat caagcggttc tccaacagcg ggcatctgca
      541 catcggcagt gtggaccaca tgggccgggt ggggctgact acccccatct ccaccttttt
      601 gaagcagtat cccgacggcg gcgtcaccat cgacatcgag cgggggacct ccctgggggt
      661 gatggaccag ctggtggcgg gaaagctgga tatggccttc atcgcccaca tcatctcccc
      721 cttttccaag gcgtccaaca tcgacgccta tcagctggac cactaccgcc tctataccct
      781 agtggtggac aactaccacg ccatcgtcag ccgccgccat cggttcgccg ggctggatgt
      841 ggtcacctgg caggatctgg cggaggaaaa gctggtcatt ctggacaaga gctacggtct
      901 caatgccatc atccgggaat ccttccgcca gtgcggcctg cggcccaaca tcgccttcga
      961 gtgcgaccag gtggacgcca tcctggggat ggtggaggga aatttcggga tctccatcct
     1021 ctccaagcgc attgccgcca cccagtatga cgtggaggcc atccccatcg acccgcccat
     1081 ctcccgcaac accgtgctgg tggtccccaa ggaggtggag gcccgccagc ggctggccgg
     1141 cggctttgtc cgccacatcg tggattatta cagggacaac acccccggcc cctgcgggga
     1201 gccgtagcct ccgccctccg cgcccgggcg gttcccggca ttctcccccc tcgcgcactg
     1261 tccgtcggac ggagcgcgag gggggagctt tctgtccggc cctgcctctc gacagggcct
     1321 cctccccgtg gtacaatgga caaaaccaca aaggagactg tcccgggaca tggagacaaa
     1381 cattttgatc gtagacgacg aggccgccat cgccgacctg gtggaggtct atctgaaaaa
     1441 tgagggctac accgtccaca agttcggccg cgcgggggac gccctggcct gtgtggcctc
     1501 cacgccgctt gacctggcca tcctggatgt gatgctcccg gatatggacg gcttcaccct
     1561 gtgccagaag atccgggagg agcacctgtt ccccatcctg atgctcactg ccagggtgga
     1621 ggacatggac aagatcatgg ggctgaccct gggggcggac gactacctca ccaagccctt
     1681 caatcctctg gagctggtgg cccgggtcaa gacccagctc cggcggtaca cccggtacaa
     1741 ccagggcggc cagccggggg gagaggtgca ggagcacgac ttccgggggc tccagatctc
     1801 ccgggccacc cacaagtgcg tgctgttcgg cgaggagctc cccctgaccc ccctggaatt
     1861 ttccatccta tggcacctgt gccagcaccg ggggcaggtg gtgtccagcg aggagctgtt
     1921 cgagtcggtg tggggagaga agtatatgga cagcaacaac accgtcatgt cccacatcgc
     1981 ccgcctgcgg gagaagatgc acgaacccag ccgaaagccg aaattcatca agactgtgtg
     2041 gggggtgggc tacaccattg agtaagaaac gcaagccccg tctggcgcag cggctccaga
     2101 cagaccgtgg gctctaccgc gccctccagc gctgggggta ttatctgctg gccctgatgg
     2161 cctgggtgac cgtggtgctg gcggtctccg ccctgggggt cctggtgtgc agccgcctta
     2221 cctggtatga gaccgaccct ctctaccacc tgctcctgtg ggtccgggac tactacttct
     2281 ttttctgcat agcggtggtc ctgctgggct gggttgtcat cagctggcac ttcatctccc
     2341 gcccagtccg ccagctggat gcgctgatcg gcgcctccgc ccagctcacc cgtcccacgg
     2401 aggagcccat ccgcctgccc gcctccatga agagcgtgga ggaccagctg aacctggccc
     2461 gggaggaggc cctgcgtgcc cagcgggccg cccgggaggc ggaacagcgg aaaaacgacc
     2521 tggtggtcta tctggcccac gacctgaaga cccccctcac cagcgtcatc ggctatctga
     2581 cactgctccg tgacgagccc cagctctccc cggagctgcg cgcccgctac accggcatcg
     2641 ccctggacaa ggcggagcgt ctggaggagc tcatcaacga attctttgag atcacccgct
     2701 tcaacctcag ccatctggag ctggagaagc agcccgtgga cctgtgccgg atgctccagc
     2761 aggtggtcag cgagttcgcc cccatgctgg gggagcggga cctgacctgc gccctggagc
     2821 ttccccccag cctggaatat gactgcgacc cggacaagct ggcccgggtg tttgacaacc
     2881 tgctccgcaa cgcctgccat tacagcactc ccggcagcgt ggtccgcatc tccggcgggg
     2941 aggaggggga ttccattgtc ctcaggttcc tcaactccgg gcggaccatc cccccggaga
     3001 agctggcgcg catctttgac cagttcttcc gcctggacag ctcccgcgcc acccggacgg
     3061 ggggcgcggg ccttggcttg gccatagcca aggagatcct gactctccac ggcgggacca
     3121 ttaccgcctg gagccgggag gatcaggtct gcttccaggt gacgctaccc cggggcggac
     3181 ggcgggagga gggtccagcc cgtgagccat gacgccgccc ccctgttcgc aggctgggag
     3241 aaggggatga tcct
//
LOCUS       DBMDSJ010000177         3253 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7385_length_3253_cov_1.355647, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000177 DBMDSJ010000000
VERSION     DBMDSJ010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7385_length_3253_cov_1.355647"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>465
                     /locus_tag="ACZPAQ_05270"
     CDS             <1..>465
                     /locus_tag="ACZPAQ_05270"
                     /inference="COORDINATES: protein motif:HMM:NF020075.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR0903429.1"
                     /translation="QVRVGAYLSKEDALDAADELGEGEVVGTSAYAVTVIQTGTDRVL
                     FQFDGGEDLALGIMPDVTGEDEVRTWFQGYKYHGGFRYERIGGGDLTVVSVVDMETYI
                     KGVIPFEMSNDWPLEALKAQAICARSYAYNNISQNKHSAHHFDVCSSTDCQV"
     assembly_gap    466..475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <476..1314
                     /locus_tag="ACZPAQ_05275"
     CDS             <476..1314
                     /locus_tag="ACZPAQ_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR0903430.1"
                     /translation="GSNVSSYQSTDRTDRAVEETAGEYALYDGTVIEAFYSSSHGGAS
                     EDVYNVWGSSREKYPYLCGVEDPYEQDVASLNSYSSWTVRYTASELTSLLQKWGGVNA
                     SVESLTLTYSDLGNVIQVKVNYTNGESSTIRQRSTDLRNLFGLPSIRFTINGAEAEPG
                     SPSGGSLSGEGLSGRSRLYTISGTGALSREDRDDLYVISGAGSIDRMGEQTGSSGGDT
                     GAVAGTEVTVSGSQYVFQGSGWGHQLGMSQYGAYAMAERGFDYDEIVEFYFPGVEVDA
                     LD"
     gene            1339..2391
                     /gene="queA"
                     /locus_tag="ACZPAQ_05280"
     CDS             1339..2391
                     /gene="queA"
                     /locus_tag="ACZPAQ_05280"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR0903431.1"
                     /translation="MKTSDFDFELPEELIAQTPLERRDASRLLTLDKYTGETGHHHFY
                     ELPRFLRPGDCLVLNDSRVLPARLIGRRSTGGACEVLLLIDKGEGLWECLVRPGRKMK
                     PGAQLSFGEGKLTATVEAELPGGNRLVRFHYQGIFLEILEELGRMPLPPYIKAELQDN
                     ERYQTVYSKVLGSAAAPTAGLHFTPELLRQVEEMGVRLCYVTLHVGLGTFRPVKEEEI
                     TDHEMHSEYCMVPGETARIINETRKNGGRVICVGTTSCRTVESFAAEDGTMEESAGWT
                     NIFIYPGYRFKVLDGLITNFHLPESTLIMLVSALAGREHVLAAYREAVRERYRFFSFG
                     DAMLIADFKEQEREKG"
     gene            2470..>3253
                     /gene="tgt"
                     /locus_tag="ACZPAQ_05285"
     CDS             2470..>3253
                     /gene="tgt"
                     /locus_tag="ACZPAQ_05285"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR0903432.1"
                     /translation="MFQILKTEGRARRGVFTCAHGTAQTPVFMNVGTQGAIKGAVSSH
                     DLKTLGCQIELSNTYHLHLRPGDGVVRQLGGLHQFMDWDGPILTDSGGFQVFSLSGLR
                     KITEEGVTFASHIDGRRIFMGPEESMQIQSNLGSDIAMAFDECIENPAPFDYVEASCA
                     RTYRWLERCVAEHQRLNRQPGCVNPQQMLFGINQGGTYPELRVRHMEQIAQLPCDGFA
                     IGGLAVGEPTQTMYDIIDAVEPHMPREKPRYLMGVGTPSNIIE"
BASE COUNT          617 a          980 c         1102 g          544 t
ORIGIN      
        1 tcaggtccgg gtgggagcct acctctccaa ggaggacgcc ctggacgccg ccgatgagct
       61 gggcgaaggg gaggtcgtgg ggaccagcgc ctatgccgtc acagtgatcc agacggggac
      121 cgaccgggtc ctgttccagt ttgacggggg agaggacctg gccctgggca tcatgccgga
      181 cgtgaccggc gaggatgagg tgcggacctg gttccagggg tacaagtacc acggcggctt
      241 ccgctatgag cgcatcggcg gcggagacct gacggtggtc agcgtggtgg acatggagac
      301 ctacatcaag ggggtgatcc cctttgagat gagcaacgac tggcccctgg aggcgctgaa
      361 ggcccaggcc atctgcgcca ggagctatgc ctacaacaac atctctcaga acaagcacag
      421 cgcccaccac ttcgacgtgt gcagttccac ggactgccag gtctannnnn nnnnnccggc
      481 tccaacgtca gcagctatca gtccacagac cgcactgacc gggcggtgga ggagacagcg
      541 ggggagtacg ccctctatga cggcacggtg atcgaggcgt tctactcctc cagccacggc
      601 ggggccagcg aggacgtgta caacgtctgg ggctcctccc gggagaaata tccctacctg
      661 tgcggggtgg aggaccccta tgagcaggat gtggccagcc tgaattccta ctcctcctgg
      721 accgtgcgct atacggcctc ggagctgacc agcctgctcc agaaatgggg gggcgtgaac
      781 gcctcggtgg agtcgctgac cctgacctac tcggacctgg gcaacgtgat ccaggtgaag
      841 gtcaattata ccaacgggga gagcagcacc atccgccagc ggagcactga cctgcgcaat
      901 ctgttcggcc tgccctccat ccgcttcacc atcaacgggg cggaggcgga gcccggttcc
      961 ccctccggcg ggagcctgag cggggaaggg ctgagcggca ggagccgcct ctacaccatc
     1021 agcggaacag gagccctgag ccgggaggac cgggatgacc tgtatgtcat ctccggggcg
     1081 gggtccatcg accggatggg agagcagacg gggagctccg gaggagacac aggcgctgtg
     1141 gcgggcaccg aggtcacggt gagcggcagc cagtatgtat tccaaggctc cggctggggc
     1201 caccagctgg gcatgagtca gtacggtgcc tacgccatgg cggagcgggg attcgactac
     1261 gacgagatcg tggaattcta tttcccgggg gtcgaggtcg atgccctgga ctaagagaaa
     1321 agaggacaag gaagtacatt gaagacatct gattttgatt ttgaactgcc ggaggagctg
     1381 atcgcccaga ctcctctgga gcgcagggat gcttcccgcc tgctgacgct ggacaagtac
     1441 acgggcgaaa cgggccacca ccatttctat gagctccccc ggtttttgag accaggggac
     1501 tgcctggtgc tcaacgactc ccgggtactg cccgcccggc tgatcggccg ccggtccacc
     1561 ggcggcgcct gcgaagtgct gctcctcatc gacaaagggg agggcctgtg ggagtgtctg
     1621 gtccggccgg ggcggaagat gaagcccggc gctcagctct ccttcgggga ggggaagctc
     1681 accgccacgg tggaggcgga gctgcccggc gggaaccggc tggtccgctt ccactaccag
     1741 gggatctttc tggagatcct ggaggagctg ggccggatgc ctctgccccc ctatatcaag
     1801 gcggagctcc aggacaacga gcggtatcag accgtctact ccaaagtgct ggggtccgcc
     1861 gccgctccca ccgccggcct gcacttcacg ccggagctgc tgcgccaggt ggaggagatg
     1921 ggggtcaggc tgtgctatgt gacgctccat gtggggctgg ggaccttccg cccggtgaag
     1981 gaggaggaga tcaccgacca tgagatgcac tccgagtact gcatggtccc cggggagacc
     2041 gcccggatca tcaacgagac ccggaagaac ggggggcggg taatctgtgt gggcaccacc
     2101 tcctgccgca cggtggagag ctttgcagcg gaggacggga ccatggagga gtctgccggc
     2161 tggacgaaca tcttcatcta tcccggctac cgcttcaaag tgctggacgg gctgatcacc
     2221 aatttccacc tgccggagtc cacgctgatc atgctggtct ccgccctggc aggccgggag
     2281 cacgtgctgg ccgcctaccg ggaggcggtg cgggagcgat accggttttt ctccttcgga
     2341 gacgcgatgc tgatcgcgga cttcaaagag caggaaaggg aaaagggctg acagaaagct
     2401 gcgggctttt tccggcggat caaaggcccc gccccggcgg ggaacaagat acggtaaagg
     2461 agtttttttg tgtttcagat cctgaagacg gagggcaggg ccaggcgtgg cgtgttcacc
     2521 tgcgcccatg gaactgccca gacccctgtt tttatgaatg tcgggaccca gggggccatc
     2581 aagggggcgg tctcctccca cgacctgaag acgctgggct gtcagatcga gctgtccaac
     2641 acctaccacc tccacctgcg gccgggagac ggggtggtgc ggcagctggg cggactgcac
     2701 cagtttatgg actgggatgg ccccatcctc accgacagcg gcggatttca ggtcttttct
     2761 ctgtccggcc tgcgtaagat aacggaggag ggcgtcacct tcgcctccca tatcgacgga
     2821 cgtcggatct tcatgggacc ggaggagtcc atgcagatcc agtccaatct gggcagcgac
     2881 atcgccatgg cctttgacga gtgcatcgag aaccccgccc ccttcgacta cgtggaggcc
     2941 tcctgtgccc ggacctaccg ctggctggag cgctgcgtgg cggagcacca gcggctgaac
     3001 cggcagcccg gctgtgtcaa tccccagcag atgctcttcg gcatcaacca aggcgggacc
     3061 tacccggagc tgcgggtgcg gcacatggag cagatcgccc agctgccctg tgacggtttc
     3121 gccatcgggg ggctggcggt gggggagccc acgcagacca tgtacgacat catcgacgcg
     3181 gtggagcccc atatgccccg ggagaagccc cgctacctca tgggggtggg gaccccctcc
     3241 aacatcatcg agg
//
LOCUS       DBMDSJ010000178         3249 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7396_length_3249_cov_2.132783, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000178 DBMDSJ010000000
VERSION     DBMDSJ010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7396_length_3249_cov_2.132783"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(81..797)
                     /locus_tag="ACZPAQ_05290"
     CDS             complement(81..797)
                     /locus_tag="ACZPAQ_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0903433.1"
                     /translation="MNKPHILMVEDDAAVGNLIATTLETQNYQFHRAKTGASALVDAL
                     SYRPDVMLLDLGLPDMDGVDIIKKVRTWSNLPIIVISARSEDSDKVAALDAGADDYLT
                     KPFSVDELLARLRVALRRVRYDSQKVSEEASLYENGNLKIDYAAGCAYRDGVEIHLTP
                     IEYKLLCLLARNTGKVLTHNFILNEIWGSSAFSDTPSLRVFMATLRKKLEVDPSHPQY
                     IQTHIGVGYRMIRQKQEDEA"
     gene            complement(790..>3249)
                     /locus_tag="ACZPAQ_05295"
     CDS             complement(790..>3249)
                     /locus_tag="ACZPAQ_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR0903434.1"
                     /translation="TLALAGGLERLPPLIPPGGGQGEFDLDGALRRRPQLILVDELAH
                     SNNKGARHAKRYQDIAELLRAGIDVYSTMDVQQLESLSDVVSSITGFSAPERVPDQVF
                     DSADQVEIVDLEPSDLLARLRAGTIGGGEGHLLTEQTLAALRELSLRRTADRLERKPS
                     GGDRYPPRAREHILICLSGSPSNEKVIRTAARMAEAFHGAFTALFVETPSFQGQSDKE
                     RRQLRNNLHLAEELGARISTVYGDDPAIQIAEYAKVSGISKIVLGRSPQKRGPLLGAK
                     SLMDRLGELAPDLDIYIIPDQVQRPARHSWQPYLLQERFSVRDLFKALLILAACTAVG
                     FLFQRAGFSTENVIMVYILGVLTISMATTGHSYSLLASLCSVFIFNYFFTYPYFTLMS
                     DPSYIATFAVMLTVALLGSSLTTRVKRQAIQSAQKAYRTEILLETSQKLQKAEGSREI
                     LAVTATQLKKLLERDVLIYSADEDGLEQEAMLFPVSKGGDMSAYLTSSERAVAEWVWN
                     NNKHAGATTHTLPSSKCLYIAVRGSDKVLAVVGIAIPNAQKVDSFEKNLTVAILDECG
                     LALEKELTARAKQQIEELAHQETLRANLLRAISHDLRTPLTSISGNAGILMENGAVLD
                     EEKRRYLYRSIYDDSMWLINLVENLLSLTRMGNGSVSLNIRPELLDEVFHEALSHLDR
                     NAEQHHISVELEDDLLMADMDARLIVQVVINLVNNAIKYTPAGSHISLSAVRAGPFVQ
                     VEVSDDGPGIADSAKQKIFDMFYTADNSHGDGRRGLGLGLSLCRSIITAHGGEISVQD
                     HQPHGSVFRFTLRASEVKMYE"
BASE COUNT          637 a          923 c         1055 g          634 t
ORIGIN      
        1 caccgcccgc tgggaagaaa aagactgccc gccccctgca agacgcaggg aacggacagt
       61 cagggacgca ggtgcagagg tcaggcctcg tcctcctgct tctggcggat catgcggtag
      121 cccacgccga tgtgggtctg gatatactgg gggtggctgg ggtccacctc cagctttttg
      181 cgcagagtgg ccatgaagac gcggagagag ggggtatccg aaaaggcgga gctgccccaa
      241 atctcgttga ggataaaatt gtgggtgagg accttcccgg tatttcgggc caaaaggcag
      301 agtagcttgt actcgatggg ggtgagatgg atctccaccc catctcgata ggcacagccg
      361 gcagcatagt cgatcttcag gttcccgttc tcgtagaggc tggcttcctc acttaccttc
      421 tggctgtcat agcggacccg gcgcagggcg acccggagcc gggccaggag ctcgtccacg
      481 gagaaaggtt tagtcagata gtcatcggcg ccggcgtcca gcgcggccac cttgtcgctg
      541 tcctcgctcc gggcggagat gacgatgatg ggcaggttgc tccaggtgcg gacctttttg
      601 atgatgtcta ccccgtccat gtcgggcagg cccaggtcca gcagcatgac gtccgggcgg
      661 taagagaggg cgtccaccag agcactggcc cctgtcttgg cccgatggaa ctgatagttc
      721 tgggtttcca gagtggtggc gatgaggttt cccaccgctg cgtcgtcttc taccataaga
      781 atgtgcggct tattcataca tttttacctc cgaggccctc agggtgaaac gaaatactga
      841 cccgtggggc tgatgatcct gaactgatat ctcaccgccg tgagcggtga tgatggacct
      901 gcacagggac agccccaggc ccagcccccg ccggccatct ccgtgggagt tgtccgcggt
      961 ataaaacata tcaaatattt tctgcttggc gctgtccgca atacctggac cgtcatcgga
     1021 gacctcgacc tgcacaaaag gaccggcccg gacagcggac aaggagatgt gggaaccggc
     1081 aggggtgtac ttgatggcgt tgttgaccag attgatgacc acctggacga tgagccgggc
     1141 gtccatatcc gccatcaaca gatcgtcctc cagttctaca gagatatggt gctgctccgc
     1201 attgcggtcc agatgggaca gggcttcatg aaagacctca tccagaagct ccgggcggat
     1261 gttcagagaa acagagccgt tgcccatccg ggtgagggag agcagatttt ccaccaggtt
     1321 gatgagccac atggagtcat cgtagatgga gcggtacagg tagcgccgct tctcctcatc
     1381 cagcacggcc ccgttctcca tcaggatgcc ggcgttcccg gatatactgg tcagtggggt
     1441 gcgcaggtcg tgggagatgg cccgcagcag attggcgcgc agggtctcct gatgggccag
     1501 ctcctcgatc tgctgcttgg cgcgcgccgt cagctccttt tccagcgcca gtccgcactc
     1561 gtccaggatg gctactgtca gatttttttc aaaggagtcc accttctgag cattggggat
     1621 ggcaatgcca accaccgcca gcaccttgtc agagccgcgg accgcaatgt agaggcattt
     1681 ggagctggga agggtgtggg tggtagcgcc ggcgtgcttg ttgttgttcc agacccattc
     1741 cgccaccgcc cgctctgagg aagtgagata tgcagacata tccccgcctt tggaaacggg
     1801 gaacagcatg gcctcctgct ccagtccgtc ctcgtcggcg gaatagatca ggacgtcccg
     1861 ctccagcagc tttttcaact gggtggccgt gaccgccagg atctcccggc tcccctccgc
     1921 cttctggagc ttctggctgg tctccagcag gatctcagtg cggtatgcct tttgggcgct
     1981 ctggatggcc tgccgtttga cacgggtggt aagagagctg cccagcagcg ctacggtcag
     2041 catgacggca aaggtggcga tatagctggg gtcgctcatc aaagtaaaat aggggtaggt
     2101 aaaaaagtaa ttgaagatga agacggagca gagggatgcc agcaggctgt agctgtgccc
     2161 cgtggtggcc atggagatgg tgaggacgcc caagatatag accatgatca cattttcagt
     2221 ggaaaagccc gctcgctgga agagaaagcc cacggcggtg caggcagcca ggatcaggag
     2281 ggccttgaaa agatctcgga cggagaagcg ctcctggaga agatagggct gccaggagtg
     2341 gcgggcgggc cgctggacct ggtccgggat gatgtagata tccaggtcag gagccagctc
     2401 tcccagacgg tccatcagac tcttggcgcc caggaggggg ccgcgtttct ggggactgcg
     2461 gcccagcacg atcttggaga tcccgctgac tttagcgtat tccgcgatct gaatggcggg
     2521 gtcatccccg tagaccgtgc tgatccgggc gcccagctcc tctgcaagat gcagattgtt
     2581 ccgcagctgc cggcgttcct tgtcgctctg gccctgaaag gaaggagtct ccacgaaaag
     2641 cgcggtaaag gcgccgtgaa atgcctctgc catcctggca gcagtgcgga tgaccttctc
     2701 gttggaggga gagccggaca ggcagatgag aatgtgttcc cgggccctgg ggggatatct
     2761 gtccccaccg gagggcttcc gctccaggcg gtcagcggtc ctgcgcaggg agagctcccg
     2821 cagggcggcc aaggtctgct cggtcaaaag gtgcccttcc cctccgccga tggtcccggc
     2881 ccgcaggcgc gccagaaggt cggagggctc cagatcgaca atctccacct ggtcagcaga
     2941 gtcaaagacc tggtccggga cgcgctccgg ggcggagaag ccggtgatgg aggagaccac
     3001 atcgctcagg ctttccagct gctgcacatc catggtggaa tagacgtcaa tgcccgcgcg
     3061 aagcagctcg gcaatatcct ggtagcgctt ggcgtggcgg gctcccttat tgttggagtg
     3121 ggccagctca tccactagga tcagctgggg acggcggcgg agggcgccgt ccaggtcaaa
     3181 ttcgccctgc cctccaccgg gagggatgag gggcgggagc cgttccagtc ctccggccag
     3241 tgccagggt
//
LOCUS       DBMDSJ010000179         3244 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7408_length_3244_cov_2.013363, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000179 DBMDSJ010000000
VERSION     DBMDSJ010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7408_length_3244_cov_2.013363"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="ACZPAQ_05300"
     CDS             complement(<1..2232)
                     /locus_tag="ACZPAQ_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase-related domain protein"
                     /protein_id="HIR0903435.1"
                     /translation="MHEKLIREINGEIADALLARKMPFAPSRKAALALLKVPGWTEGL
                     EQMLPIRGRLECAHVLELCSCVLPRLAPRPEEGWLAFCTQYARERMYPGQGFAPDREE
                     YEAGALFFLTVLQVMLDRERRAVPFDPLKDFQFLSSEEMGEYECGEEYRRFLAAFREE
                     YVYEMMRLSEETTPFRTLGHIAGVHYIAMTVARGIREAGENVDLALVSAAAAAHDLGK
                     FGCRPGERVPLLHYYYTDQWLLGRGMPAVSHIAANHSTWDLELDTLSMESLCLIYADF
                     RSKQGRDEQGRETTILYPLEESFHIILRKLENVDQAKRRRYEFVYGKLHDFEDYMRSL
                     GVDVDLTGQPGARKPPKDPALMPPDETVDSLILLSVEHNLKLMHQLSSERKFGNIIEA
                     ARSAKSWQQLRAYLDVFDEYCTYLSVRQKTQALAFLYELLIHREGDIRRQAASLIGKI
                     IAKFHLVYRKEVPADARTDPAEEVPFTLWEEYLDKLIYPDHKTTPQQRGQIGYTLKLV
                     VDALLEYGRSETMPRFIGALLRYYDRPEEKDEDTAFTLLDAIRYLPAQQYDQETRGRL
                     IRFAAWFAASGNLRLQAAALQFLREAQRSLPQDHPQMAQIAAAARSAAGGSLPLLFLR
                     YKVLQRAGEDVSDYQAALYEQDVTSEIFLDNLKSATHWIIKVVGIELLQDQVEHGLRE
                     HMLHIATHFSNLVKVSERVGVRHAAGEALVRLIPLLRREQRNEVVVELGKGLEMGQYQ
                     ISKY"
     gene            2477..3133
                     /locus_tag="ACZPAQ_05305"
     CDS             2477..3133
                     /locus_tag="ACZPAQ_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR0903436.1"
                     /translation="MKVLLINGSPHKEGCTYTALSEVAAQLQKHEIETEIFWIGNQPI
                     RGCIDCRSCWGKGRCVFDDDSVNQCIQKIIQADGLVVGSPVYFGGIAGGLKCLLDRVF
                     YDAKRLFFYKPAAAVVTCRRGGAGNTFDQLNKYFTISSMPVVSSQYWNGVHGDRPEEV
                     RADLEGMQTLRLVGDNMAWLLRSMTAAREAGVVPYEREVRVKTNFYKPEAAPNVQGEG
                     "
BASE COUNT          546 a         1060 c         1036 g          602 t
ORIGIN      
        1 atacttggag atctggtact ggcccatctc caggcccttg cccagctcca ccaccacctc
       61 gttgcgctgc tcccggcgca gcagggggat caggcggacc agggcctcgc cggcggcgtg
      121 gcggacgccc acccgctcgg acaccttcac caggttggag aagtgggtgg cgatgtggag
      181 catgtgctcc cgcaggccgt gctccacctg gtcctggagc agctcgatgc ccactacctt
      241 gatgatccag tgtgtggcgc tcttcaggtt gtccaggaag atctcactgg tcacgtcctg
      301 ctcatacagg gccgcctgat agtcggacac gtcctccccc gcccgctgga ggaccttata
      361 ccgcaggaag agcagcggca ggctcccgcc ggcggcagac cgggcggccg ccgcgatctg
      421 agccatctgg gggtgatcct ggggcaggga gcgctgggcc tcccgcagga actggagggc
      481 cgccgcctgg aggcgcaggt tgccggaggc ggcgaaccag gccgcaaagc ggatgaggcg
      541 gccccgggtc tcctggtcat attgctgggc gggcagatag cggatggcgt ccagcagggt
      601 gaaggcggta tcctcgtcct tctcctccgg ccggtcatag tagcgcagca gcgccccgat
      661 gaagcggggc atggtctcgc tccggccgta ctccagcagg gcgtccacca ccagcttcag
      721 ggtatagccg atctggcccc gctgctgggg ggtggtcttg tggtcggggt agatgagctt
      781 gtccagatac tcctcccaca gggtgaaggg cacctcctcc gccgggtcag tgcgggcgtc
      841 ggcaggcacc tccttgcggt agaccaggtg gaatttggcg atgatctttc cgatgaggct
      901 ggctgcctgg cgccggatat ccccctcccg gtggatcagc agctcataga ggaaggccag
      961 ggcctgggtc ttctgccgca cggacagata ggtgcagtac tcgtcgaaca catccagata
     1021 ggcccgcagc tgctgccagc tcttggccga gcgggcagcc tcgatgatgt tgccgaactt
     1081 ccgctcgctg ctcagctggt gcatcagctt caggttgtgc tctaccgaca gcaggatcag
     1141 gctgtccacc gtctcgtccg ggggcatcag ggccgggtcc ttggggggct tccgcgcccc
     1201 cggctgcccg gtcaggtcta cgtccacccc cagagagcgc atgtagtcct cgaaatcgtg
     1261 gagcttgccg tagacgaact cataccggcg gcgctttgcc tggtccacat tctccagctt
     1321 cctcaggatg atgtggaagg actcctccag gggatagagg atggtggtct cccgcccctg
     1381 ctcatcccgt ccctgcttgc tgcggaagtc ggcgtagatc agacacaggg actccatgga
     1441 gagggtgtcc agctccaggt cccaggtgga gtggttggca gcgatgtggc tgacggcggg
     1501 catcccccgc cccagcagcc actggtcggt gtagtagtag tggaggagcg ggacacgctc
     1561 cccgggccgg cagccgaact tgcccaggtc gtgggcggcg gcggcggcgg acaccagcgc
     1621 caggtctacg ttctcccccg cctcccggat gccccgggcc acggtcatgg cgatgtaatg
     1681 cacccccgcg atgtgcccca gggtgcggaa gggggtggtc tcctcgctca gccgcatcat
     1741 ctcatagacg tactcctccc ggaaggcggc caggaagcgg cggtactcct ccccgcactc
     1801 atattccccc atttcctctg aggagaggaa ctggaagtcc ttcagggggt cgaagggcac
     1861 cgccctccgc tcccggtcca gcatcacctg gaggacggtg aggaaaaaca gggcccccgc
     1921 ctcatactcc tcccgatcag gggcgaagcc ttgaccgggg tacatccgct cccgggcgta
     1981 ctgggtgcag aaggccagcc acccctcctc cggccggggg gcgagccggg gcagtacaca
     2041 ggagcacagc tccagcacat gggcgcactc cagccgcccc cggatgggca gcatctgctc
     2101 cagcccctcc gtccatcccg gcaccttcag cagggccagc gccgccttcc ggctgggggc
     2161 aaagggcatc ttccgcgcca gcagcgcgtc ggcgatctcc ccgttgatct ctcggatcag
     2221 cttctcatgc ataggctccg cctctcttct cctccgaggc cggcctgtcc gcgctcaggg
     2281 ccgcctccgg gttatttttt tattgtatca cgcggagcac gcccggtaaa gaatctttcg
     2341 cctgggagac tcccattttt tcccgctttt gcccgatctc tcccgtgttt tcgattctca
     2401 ttgcaaatcc tggcccggcg cggtacaatg ggagcatatg ggaaagggct caagatgccc
     2461 aaaggaggag atacgtgtga aagtcctgct catcaacggc agcccccaca aagagggctg
     2521 cacctacacc gccctgtccg aggtggcggc tcagctccag aagcatgaga tcgagaccga
     2581 gatcttctgg atcggcaatc agcccatccg agggtgtatc gactgcagat cctgctgggg
     2641 caagggccgc tgtgttttcg acgatgacag cgtcaatcag tgcatccaga agatcatcca
     2701 ggccgacggc ctggtggtgg gctccccagt ctatttcggc ggcatcgcgg gggggctgaa
     2761 gtgcctgctg gaccgggtgt tctacgacgc caagcggctg ttcttctaca aacctgccgc
     2821 cgccgtggtc acctgccgcc gtggaggggc cggcaacacc tttgaccagc tcaacaagta
     2881 cttcaccatc tcctccatgc cggtggtgtc ctcccagtat tggaacggcg tccacgggga
     2941 ccggccggag gaggtccggg cagatctgga ggggatgcag accctgcgcc tggtggggga
     3001 caatatggcc tggctgctcc gctccatgac cgccgcccgc gaggccggcg tggtccccta
     3061 tgagcgggag gtccgtgtga agaccaactt ctacaagccg gaggccgccc ccaatgtcca
     3121 gggggagggc tgagctcctc atcccctctt cttttacatc ctgcatggaa tatccaaaaa
     3181 aacatcggga gcagccttag gctgctcccg atgtttttgc acttattcgg cggagatcat
     3241 gtaa
//
LOCUS       DBMDSJ010000180         3220 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7468_length_3220_cov_1.677140, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000180 DBMDSJ010000000
VERSION     DBMDSJ010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7468_length_3220_cov_1.677140"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            122..1045
                     /locus_tag="ACZPAQ_05310"
     CDS             122..1045
                     /locus_tag="ACZPAQ_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0903437.1"
                     /translation="MHSFYLFLWIFFIYSFLGWCLEVCFFALNTGKFVNRGFLNGPVC
                     PIYGFGVVIVVLCLTPLMENTLLLFLGSVLLTSLLELGVGFALEKLFHQRWWDYTNEP
                     FNLGGYICLRFSLAWGLACLLVMEIIHPSVLWLILHIPHTLGLVLLACFVSVMAVDLT
                     ATVRTIARINRSLGQIDELAGRIKALSNDLGENLADRVLDAAEKGADWKESLADRRED
                     LMEDLASWKEELAEQRSEYQLDLALRRAQRLEELEQLRKSLSELLDRHTFGQRRLLRA
                     FPQMRSIDHKQALERLRQRQKERQEKKGGSV"
     gene            1114..1647
                     /locus_tag="ACZPAQ_05315"
     CDS             1114..1647
                     /locus_tag="ACZPAQ_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR0903438.1"
                     /translation="MDELLDLKQRLEQERPTPWAELPDLALYMDQIISYMPRQLIHFD
                     DGEVLTSAMVNNYIKDGLVPRADGKRYGPIHLGYLTAVCALKRVLSVRDMKVLVSAGE
                     KREKPPEELYAYFLQALDRALNETAQSLPPQAAPEDLPRLALDLALRSYASQLACARI
                     LDILQPPCEEAPKKAKK"
     gene            1708..2580
                     /locus_tag="ACZPAQ_05320"
     CDS             1708..2580
                     /locus_tag="ACZPAQ_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR0903439.1"
                     /translation="MSDFIILTDSSADLSGEMAQAADVQVLPLTFTIGGADYHNYPDN
                     REMDPHLFYQRLRAGEVATTAAVNMNQYVEAIEPLLQAGRDVLVLAFSSGLSATFQSS
                     KLAVEELSEKYPERKLYTVDTLCASLGQGLLVWYAAQERKKGRTIEEVRDWAEANKLS
                     LCHQFTVDDLHFLKRGGRISATTAVVGSMLQIKPVLHVDDEGHLINIGKARGRQSSLK
                     ALVDKMEKTVTEAGKQTVFISHGDCLEDAEAVAQMVRERFGTQDIRINYVGPVIGAHS
                     GPGTLALFYLGTQR"
     gene            2616..>3220
                     /locus_tag="ACZPAQ_05325"
     CDS             2616..>3220
                     /locus_tag="ACZPAQ_05325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR0903440.1"
                     /translation="MDEIKWLEVAVNTTPDRLDEVTAKLTAAGMTGLVIEDEGEFQQF
                     LEQNRQYWDYVDQELMDRMRGVTRVKFYVTDDGAGRVQLEQYTRGLDAEYTVTPLTDN
                     DWAYSWQKYYKPLEVGRRLYVVPEWMRDEPVPEGRSPLYLNPGLTFGTGSHASTQLCL
                     EGVEEHTLTGRPVLDLGCGSGILSIAALCLGASTAAAVDIDP"
BASE COUNT          584 a         1050 c          959 g          627 t
ORIGIN      
        1 gtccttttcg tcctgtcaag tattcccaca aattttctgg actttccccg gcaaatcggc
       61 tatacttctt aacagacaga catggacgca tcccacgcag cctgagggca ggaggtatct
      121 tttgcattct ttttatttgt ttttatggat cttttttatt tattcctttc tcggctggtg
      181 cctggaggtc tgctttttcg ccctgaacac cggtaaattt gtcaaccgcg gctttctcaa
      241 cggcccggtc tgtcccatct acggctttgg cgtggtcatc gtggtcctct gcctcacccc
      301 tcttatggag aacacactcc tgctctttct gggctcggta ctcctcactt ccctgctgga
      361 actgggggtt ggcttcgcgc tggagaagct tttccatcag cgctggtggg actacaccaa
      421 cgagcctttc aacctgggcg gctatatctg cctgcgcttc tccctggcct gggggctggc
      481 ctgcctgctg gtgatggaga tcatccaccc ctcggtgcta tggcttatcc tccacatccc
      541 ccacaccctg ggcctggtcc tgctggcctg cttcgtctct gtcatggcgg tggacctgac
      601 cgccaccgtg cgcaccatcg cccggatcaa ccgcagcctg ggccaaatcg acgagctggc
      661 cggccgtatc aaggctctct ccaatgattt gggcgaaaat ttggctgacc gggtgctgga
      721 tgcagccgag aaaggtgcgg actggaagga gtccctggcc gaccggcgtg aggatctgat
      781 ggaggatctg gccagttgga aagaggagct ggccgagcag cgcagcgaat atcagctgga
      841 tctggcgctg cgccgggccc agcgtctgga ggagctggaa cagctgcgca aatccctgag
      901 cgagctgctg gaccgccaca ccttcggtca acgccgcctg ctgcgggctt tcccccagat
      961 gcgctccatc gaccacaagc aggccctgga gcggctgcgc cagcgtcaga aggagcgtca
     1021 ggagaaaaaa ggcgggtctg tgtgatcttc ctttgcagta tctgttattt tgtgatatac
     1081 taacgataac cacttttgaa agggggctct cccttggatg agcttttgga cctgaagcag
     1141 cggctggagc aggagcgtcc caccccctgg gcggagctgc ctgatctggc cctttacatg
     1201 gatcagatca tctcctacat gccccgccag ctgatccatt tcgacgacgg cgaggtcctc
     1261 acctctgcca tggtcaacaa ctatatcaag gacggtctgg tcccccgggc ggacgggaag
     1321 cggtacggtc ccatccatct gggctacctc accgccgtgt gcgccctgaa gcgggtgctc
     1381 tccgtgcggg acatgaaggt gctcgtctcc gccggggaaa agcgggagaa gccgccggag
     1441 gagctgtacg cctacttcct ccaggccctg gaccgggcgc tgaatgagac cgcccaatcc
     1501 ctgccacccc aggccgcccc cgaggacctg ccccgcctgg ccctggacct ggccctgcgc
     1561 agctatgcca gccagctggc ctgtgcacgc atcctggata tcctccagcc cccgtgcgag
     1621 gaggccccaa aaaaggcaaa aaagtgatct catcgcgatc ccttcggagc ggcaccgctc
     1681 cagttcaaat atcaggaggt caatcttatg tccgacttca tcattctcac cgactcctcc
     1741 gccgacctga gcggagagat ggcccaggct gcggacgtgc aggtacttcc cctcactttc
     1801 accatcggcg gggccgatta ccacaactac cccgacaacc gggagatgga cccccatctc
     1861 ttctaccagc gcctccgtgc cggcgaggtg gctaccaccg ccgcagtcaa catgaaccag
     1921 tatgtggagg ccatcgagcc cctgctccag gctggccggg acgtgctggt actggctttc
     1981 tcctccggtc tgtcggccac cttccagtcc tccaagctgg cggtggagga gctgagtgag
     2041 aagtacccgg agcggaagct gtacactgtg gacaccctgt gcgcctccct gggccagggg
     2101 ctgcttgtgt ggtacgccgc ccaggagcgg aagaagggcc gcaccatcga ggaggtccgg
     2161 gactgggccg aggccaacaa gctctccctc tgccaccagt tcacggtgga tgacctgcac
     2221 ttcctcaagc ggggcgggcg catctccgcc accaccgccg tggtgggctc catgctccag
     2281 atcaagcctg tgctccacgt ggacgacgag gggcacctca tcaacatcgg caaggccagg
     2341 ggccggcagt cctccctgaa ggccctggta gacaagatgg aaaagaccgt caccgaggcg
     2401 gggaaacaga ccgtcttcat cagccacggc gactgcctgg aggacgcgga ggccgtggcc
     2461 cagatggtcc gggagcgctt cggcacccag gacatccgca tcaactatgt gggccccgtc
     2521 atcggcgccc actccggccc cggcaccctg gccctgtttt atctgggcac tcagcgctga
     2581 gccaacttct attttcatca caaaggagcg tctccatgga cgagatcaaa tggctggaag
     2641 tggccgtcaa caccaccccg gaccgcctgg acgaggtgac cgccaagctg accgccgcgg
     2701 gcatgaccgg gctggtcatc gaggatgagg gggagttcca gcagtttctg gagcagaacc
     2761 ggcagtactg ggactatgtg gaccaggagc tgatggaccg gatgcggggc gtcacccggg
     2821 tgaagttcta cgtcaccgac gacggggccg gccgggtcca gctggagcag tacacacgtg
     2881 gcctggacgc ggagtacacc gtcacccccc tgacggacaa cgattgggcg tacagctggc
     2941 agaagtacta taaacctctg gaggtcggcc ggcgcctcta tgtagtgccc gagtggatgc
     3001 gggatgagcc cgttccagag ggccgcagcc ccctgtacct caaccccggc ctcaccttcg
     3061 gcaccggctc ccacgcgtcc acccagctct gcctggaggg ggtggaggag cacaccctca
     3121 ccggccggcc cgtgctggac ctgggctgcg gcagcggcat cctctccatc gctgctctgt
     3181 gcttgggtgc cagcaccgcc gccgcggtgg acatcgaccc
//
LOCUS       DBMDSJ010000181         3203 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7503_length_3203_cov_1.859123, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000181 DBMDSJ010000000
VERSION     DBMDSJ010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_3203_cov_1.859123"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="ACZPAQ_05330"
     CDS             complement(<1..574)
                     /locus_tag="ACZPAQ_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR0903441.1"
                     /translation="MELDRDLFLPVSREDMARRGWDFYDFLLITGDAYVDHPSFGPAI
                     IGRVLEAEGYRVAVLSQPDWRRPEAFSALGRPRLGVLISGGNLDSMVAHYTAAKKRRH
                     DDAYSPGRKAGLRPDRATIVYSNRVREVFGDIPIVIGGLEASLRRFAHYDYWEDKVRR
                     SILFDAQADLLTYGMGETASREIAARLASGT"
     gene            complement(614..1042)
                     /locus_tag="ACZPAQ_05335"
     CDS             complement(614..1042)
                     /locus_tag="ACZPAQ_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR0903442.1"
                     /translation="MHHTQEREQKIRLWLDMWLRREDLGILELFAPDAVYLESWGPEY
                     HGAAAIRHWFQEWNTRGRVEVWDSGRFFHREDATIVEWRFRCVMNDGAVQTFDGLSLV
                     EWTQEGTIRRLQEFGCNLDTYDPYRNGDPPMFREERALWF"
     gene            complement(1098..1964)
                     /locus_tag="ACZPAQ_05340"
     CDS             complement(1098..1964)
                     /locus_tag="ACZPAQ_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR0903443.1"
                     /translation="MSAKFGPAGNSDAFSAVHKSSLAAPQWIAEQGLDCYEYQCGKGV
                     HVGEDTARRVGENARKHGISLSLHAPYFINLANPDPDSLQKTIGYITSACLVASQMGA
                     SRVVIHSGALMKRTRREALDTALRSLREVIRTCDGQGFGHIALCPETMGKINQLGDLE
                     EVLELCTLDERLIPCIDFGHLYARSLGADDGAEAFGRMLDRVESVLGQSRASVFHSHF
                     SHIQFTPGGGEKCHRTFDDDDGYGPDWAPLARAIARRGWSPTFICESAGTQAKDALTM
                     KRIYQEVLTAQG"
     gene            complement(1961..2467)
                     /locus_tag="ACZPAQ_05345"
     CDS             complement(1961..2467)
                     /locus_tag="ACZPAQ_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR0903444.1"
                     /translation="MRFSPIPRGVYPGFSALDPEALAARGIRLVLADLDNTLVPYRVA
                     QPSPQLTAWRDALAAQGIALFILSNSRKPGRAQNFAQALGVPFQGRSGKPRRRGYLQA
                     MERMGCTPAQTVMVGDQIFTDTLGANNAGVTPLLVEPIQLAGNPGRYLRYAAETPFRL
                     LGKRRPFL"
     gene            2631..>3203
                     /locus_tag="ACZPAQ_05350"
     CDS             2631..>3203
                     /locus_tag="ACZPAQ_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903445.1"
                     /translation="MSMEPLIVTVPPARIGELRGLRAVPAHQGYRIGRGPHLFRAAAG
                     PVPQGGLMALDCVGFDGRGEAAPLCDEVVRECAARGFRGAVCDFEQGGVPLLDQVVRR
                     LGEGFARRGWTLYVTEPYGHSSPRARVMIPSALSGGSLSRRLEEAAERFGRERVTLAV
                     QRVAEDFFLPSPTESGVPLSRRELEEKLEQL"
BASE COUNT          525 a          975 c         1167 g          536 t
ORIGIN      
        1 gtgtgccgga ggccagccgg gcggcgatct cccgggaggc ggtctccccc atgccgtagg
       61 tgagaaggtc cgcctgggca tcgaagagga tggagcggcg caccttgtct tcccagtagt
      121 cgtagtgggc gaagcggcgc aggctggcct ccagcccacc gatgacgatg gggatgtcgc
      181 cgaacacctc ccgcacccgg ttggagtaga cgatagtggc ccggtcgggg cgcaggcccg
      241 ccttccggcc gggagagtag gcgtcatcgt ggcggcgctt tttcgccgcc gtatagtggg
      301 ccaccatgga gtccaggttc ccgccggaga tcagcacccc cagccggggg cgtcccaggg
      361 cggagaaggc ctccggccgc cgccagtcgg gctgggagag aacggccacc cggtagccct
      421 ccgcctccag tacccggccg atgatggcgg ggccgaagga ggggtgatcc acataggcat
      481 ccccggtgat gaggagaaag tcgtaaaagt cccagccccg gcgggccatg tcctcccggg
      541 agaccgggag aaaaagatcg cggtcaagtt ccatggtgaa cctcgtttct cagtgtgatc
      601 aggcatgggt ccctcagaac cagagggccc tttcctcccg gaacatgggc ggatctccgt
      661 tccggtaggg gtcataggtg tccaggttgc agccaaactc ctggagacgg cggatggtgc
      721 cctcctgggt ccactccacc agagagaggc cgtcaaaggt ctggaccgcg ccgtcgttca
      781 tcacacagcg gaagcgccac tccacaatgg tggcgtcctc ccggtggaag aagcggccgc
      841 tgtcccagac ctccacccgg ccccgggtgt tccactcctg gaaccagtga cggatggcgg
      901 cggcgccgtg gtactcgggg ccccagcttt caagataaac ggcatcagga gcaaagagct
      961 ccagaatacc caggtcttcc cgccgcagcc acatgtccag ccagaggcgg atcttttgtt
     1021 cccgttcctg cgtatggtgc atatgggtct ctcctgaaaa tataaagatt tttgagatcg
     1081 gggtctcagg agcgtcctta tccctgtgca gtcaggacct cctggtagat gcgcttcatg
     1141 gtaagggcat ctttggcctg cgtgccggcg ctctcgcaga tgaaagtggg ggaccagccc
     1201 cggcgggcga tcgcccgggc cagcggagcc cagtcagggc cgtatccgtc gtcgtcgtca
     1261 aaggtgcggt ggcacttttc tcccccgccg ggggtgaact ggatgtggga gaaatggctg
     1321 tgaaagacgc tggccctgga ctgccctaga acggactcca cccggtccag catccgcccg
     1381 aaggcctccg ctccgtcatc cgcccccagg gagcgggcgt acaggtggcc gaagtcgatg
     1441 caggggatca agcgctcgtc cagggtgcac agctccagca cctcctccag atcccccagt
     1501 tggttgatct tgcccatggt ctcgggacac agggcgatgt ggccgaagcc ctggccgtcg
     1561 caggtccgga tcacctccct cagggagcgg agggctgtgt ccagcgcctc ccgacgggtg
     1621 cgcttcatca gggcgccgga gtggatcaca acccgggagg cccccatctg actggccacc
     1681 aggcaggcgg aggtgatata gccgatggtc ttttgcaggc tgtccgggtc cgggttggcc
     1741 agattgatga aataaggggc atggagagag agggagatcc cgtgcttccg ggcgttctcc
     1801 cctaccctcc gggcggtgtc ctcccccaca tggaccccct tgccgcattg atattcgtag
     1861 cagtccagcc cctgctccgc gatccactgg ggggcggcca gggaggactt gtggacggcg
     1921 gagaaagcgt cgctgttgcc ggcgggcccg aatttggcgc tcataggaag ggcctccttt
     1981 tgcccagcag gcggaagggg gtctccgccg cgtagcggag atagcgcccc gggttgcccg
     2041 ccagctggat gggttccacc agcaaagggg taactccggc gttgttggcc cccagagtgt
     2101 cggtgaagat ctggtcgccc accatcaccg tctgagcggg ggtgcagccc atgcgctcca
     2161 tggcctgaag atacccccgg cggcggggct tgcctgagcg gccctgaaag ggcacaccca
     2221 gggcctgggc gaagttctgg gcccggcccg gcttgcggct gttggagagg atgaacaggg
     2281 cgatgccctg ggcggccagg gcgtcccgcc aggcggtcag ctggggggag ggctgggcga
     2341 cccggtaggg gaccagagtg ttgtccaggt cagccagcac cagccggatg cccctggcgg
     2401 ccagggcctc tgggtccagg gcggaaaaac cggggtagac cccccgggga atgggtgaga
     2461 atcgcaatga aggtcgtcct cctgtcggtg ttctaggatg gagcgggacg gcatagagtg
     2521 ggggtgtgag gcggcggggc cgggcggaag cccgggccgg accgctccgt tgaggttgag
     2581 atcatatcat tatatcattt ccaaagaaaa tggacaaggg ggagctagat atgagcatgg
     2641 aaccgctgat cgtcaccgtc ccgccggccc ggatcgggga gctgaggggg ctgcgggccg
     2701 tgccggccca ccagggctac cggatcggcc gggggcccca cctcttccgg gcggcggcgg
     2761 ggccggtccc tcagggcgga ctgatggccc tggactgcgt gggctttgac ggcagagggg
     2821 aggccgctcc cctgtgtgat gaggtggtgc gggagtgcgc cgccaggggc ttccggggcg
     2881 cggtgtgcga ctttgagcag ggcggggtgc cgctgctgga tcaggtggtg cggcggctgg
     2941 gggagggctt cgcccgccgg ggctggacgt tgtatgtgac ggagccctat ggacacagtt
     3001 ctccccgggc ccgggtgatg atcccgtcgg ccctgtccgg aggctcgctg tcccggcgtc
     3061 tggaggaggc ggcggagcgc ttcggccggg agcgggtgac cctggcggtg cagcgggtgg
     3121 cggaggactt ttttctgccc tcccccacag agagcggggt gcccctgagc cggcgggagc
     3181 tggaggagaa gctggagcag ctg
//
LOCUS       DBMDSJ010000182         3201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7514_length_3201_cov_1.637097, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000182 DBMDSJ010000000
VERSION     DBMDSJ010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7514_length_3201_cov_1.637097"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            32..403
                     /locus_tag="ACZPAQ_05355"
     CDS             32..403
                     /locus_tag="ACZPAQ_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0903446.1"
                     /translation="MQWQLEGDRPIYQQLVDQIMMQIVSGQRKAGDKVPAVRELAAEA
                     GVNPNTMQRALADLERQGLLYTNRTSGRYVTEDEEMITKIRERIAEERICAFLESMSQ
                     LGFTQEQVLGLIQREREEKNA"
     gene            400..1107
                     /locus_tag="ACZPAQ_05360"
     CDS             400..1107
                     /locus_tag="ACZPAQ_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903447.1"
                     /translation="MRQDNDLVVCQGLTKRFGRQTALDRLDLNLPRGKFIGLLGPNGS
                     GKTTMIKLINGLLTPTEGTVLVDGQAPGTYTHSIISYLPDRPYLSDWMRVSDLLAFFS
                     DFYKDFDQVKADEMLKDLKIGPGQRLKVLSKGTKEKVQLILTMSRKAQLYVLDEPIAG
                     VDPAARDYILGTILSNYSEEASVLLSTHLIADIERVLDEVVFIREGVMVLHKDVDAIR
                     EETGKSVDTLFREVFAC"
     gene            1101..1901
                     /locus_tag="ACZPAQ_05365"
     CDS             1101..1901
                     /locus_tag="ACZPAQ_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903448.1"
                     /translation="MLSKLIRYEWKAVARVCIPMYGGLILAAMLTHFLLGNLERFSST
                     LYDIITMAMSTLCFGLFMAAFVLTLIIQIQRFSKNLLGDEGYLMFTLPASVSQHITAK
                     LVVAVLLDVLSVAAAILAVLALALDGTMWLDLPGDFFDMIVYVDWSDWLLLLELLLLC
                     LAAGAVCMLHIYASIAVGHLARKHRTLAAFGAYFGFAVALNILFTGMLEIFVSSPWMA
                     EIGRWFMELKGDTGGHLLMWLLILGIAVLGAGFFVLTRYLLKNHLNLE"
     gene            2042..2308
                     /locus_tag="ACZPAQ_05370"
     CDS             2042..2308
                     /locus_tag="ACZPAQ_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HIR0903449.1"
                     /translation="METRIAELRYKEVISVTDGSRFGYVGDMEVDLESGQVRALVVPG
                     RLRLFGLLGREEDRYIPWDRVRRFGEDIILVEQEPQPRALRRRR"
     gene            complement(2389..>3201)
                     /locus_tag="ACZPAQ_05375"
     CDS             complement(2389..>3201)
                     /locus_tag="ACZPAQ_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR0903450.1"
                     /translation="ATYTVSVDSSRLGDVTVSPKSASKGTTVTITVKPDDGYELDELT
                     VTDKDGDSIKLKDKGDGRFTFTMPASKVTVEAVFTALEQEEEQPLFSDVAEDDWYYDA
                     VAYVAENGIMSGTDGSRFSPNGTLTRAMLSQILYAMEDKPAVSGAATFSDVAAGAWYA
                     DAVNWTAAQGIVAGMGENSFAPDAPVTREQLSLILYGYARYKGYDTSASVSLSGYADR
                     DSVAVWAADSMGWAVSEGLISGRPGGYLDPAGTATRAEVAQILMNFCEDLAR"
BASE COUNT          590 a          903 c         1098 g          610 t
ORIGIN      
        1 tacaataata cgaacggaaa ggagcacaga catgcaatgg cagctggaag gcgaccggcc
       61 catctatcag cagctggtgg accagatcat gatgcagatc gtcagcgggc agcggaaggc
      121 gggggacaag gtccctgccg tgcgggagct ggcggcggaa gccggggtaa atcccaacac
      181 catgcagcgg gcgctggccg atctggagcg ccagggcctt ctgtacacca atcggaccag
      241 cggacggtat gtgacggagg atgaggaaat gatcacaaag atcagggaac ggatcgcgga
      301 ggagcggatc tgtgcctttt tggagagcat gtcccagctg ggcttcaccc aggagcaggt
      361 cctggggctg atccagcggg agagagagga gaagaacgca tgagacaaga caacgatttg
      421 gtggtctgtc agggactgac caagcggttc ggccgccaga cggccctgga ccgtctggac
      481 ctgaatttgc ccaggggcaa attcatcggc cttctgggcc ccaacgggag tggaaagacc
      541 accatgatta agctgatcaa cgggctgctg actcccaccg aggggaccgt tctggtggac
      601 ggacaggcgc cggggaccta cactcacagc atcatcagct atctgccgga ccgcccctac
      661 ctcagcgact ggatgcgggt gtccgacctg ctggcctttt tttccgactt ttacaaggat
      721 tttgaccagg tgaaggcgga cgagatgctc aaggacctga agatcggccc ggggcagaga
      781 ctgaaggtgc tctccaaggg caccaaggag aaggtgcagt tgatcctgac tatgagccgg
      841 aaagcccagc tctatgtgct ggatgagccc atcgccgggg tggacccggc ggctcgggac
      901 tacatcctgg gcactatatt gtccaattac agcgaggagg ccagtgtcct gctctccacc
      961 cacctgatcg ccgacatcga gcgggtgctg gacgaggtgg tctttatccg ggagggcgtc
     1021 atggtcctgc acaaggacgt ggacgccatc cgggaggaga cgggcaagtc ggtggatact
     1081 ctgttccggg aggtgttcgc atgctgagca aactgattcg atacgagtgg aaggcggtcg
     1141 cgcgggtgtg tatccccatg tatggcggcc tgatcctggc ggcgatgctg acccacttcc
     1201 tgctggggaa cctggagcgg ttcagctcca cgctctatga tatcatcact atggcgatga
     1261 gcaccctgtg cttcggcctc tttatggctg ccttcgtgct gaccctgatc atccagatcc
     1321 agcgcttttc caaaaatctg ctgggggacg agggatacct gatgttcacc ctgcccgcca
     1381 gtgtgtccca gcatatcact gccaagctgg ttgtggcggt gctgctggac gtgctttctg
     1441 tggcggccgc catcctggcg gtgctggccc tggccctgga cggcaccatg tggctcgacc
     1501 tgcccggtga tttttttgac atgatcgtat atgtggactg gagcgactgg ctgctcctcc
     1561 tggagctgct cctcctgtgt ctggccgccg gcgccgtgtg catgctgcac atctatgcca
     1621 gcatcgcggt gggccacctg gcccggaagc accgtaccct ggcagccttc ggcgcctatt
     1681 ttggctttgc agtggcgctg aatatcctct ttaccggtat gctggagatc ttcgtctcct
     1741 ccccctggat ggcggagatc ggccgctggt ttatggagct gaagggggat acggggggac
     1801 acctgctcat gtggctgctg atcctgggca tcgcggtgct gggtgccggg ttctttgttc
     1861 tgacccggta tctcctgaaa aaccatctga acctggaata agggatgccg gaccgttttc
     1921 aaggggggcg ccgcggactg cgcggcgccc ccctcttttt tgcgggcagg cggggcatag
     1981 gacgggcccg cagctcataa tctgagggca ggaagaacat cccctggaag gaggtccgag
     2041 catggagacc aggatcgcgg agctgcggta caaggaggtc atcagcgtca ccgacggcag
     2101 ccggtttgga tatgtggggg acatggaggt ggacctggag agcgggcagg tccgggccct
     2161 ggtggtgccg gggcggctgc ggctgttcgg cctgctgggc cgggaggagg accgctatat
     2221 cccctgggac cgggtgcggc ggtttgggga ggacatcatc ctggtggagc aggagcccca
     2281 gcctcgggca ctgcgccgcc gccggtgaga gaggcgcgga acgggaaagg cccgccggtc
     2341 acagaccggc gggcctttcc ctatgagggg aacaaaaagg gggggaggtc agcgagcaag
     2401 atcctcacag aagttcatga ggatctgggc cacttcggcc cgggtggcgg tgccggcggg
     2461 gtccagatag cctccgggtc tgccggagat caggccctcg gacacggccc agcccatgga
     2521 gtcggccgcc cagacggcga cgctgtcccg gtcggcgtaa ccgctcaggc tgacggaggc
     2581 gctggtgtca tagcccttgt atctggcgta gccatagagg atcagggaca gctgctcccg
     2641 ggtcaccggg gcatcaggag cgaaggaatt ttcacccatg ccggccacga tgccctgggc
     2701 cgcggtccag ttcaccgcat cggcgtacca tgcgccggcg gccacatcgg agaaggtggc
     2761 cgcgccggag acagcgggct tgtcctccat ggcatagagg atctgggaca gcatggcacg
     2821 ggtcagtgtg ccgttggggg agaagcggga gccatcggtg cccgacatga tgccgttttc
     2881 ggccacatag gccactgcat catagtacca gtcgtcctct gccacatcgg agaagagggg
     2941 ctgttcctcc tcctgctcca gagccgtaaa gacggcctct accgtgacct tggaggcggg
     3001 catggtgaag gtgaacctgc cgtcgccctt gtccttgagc ttgatggagt ccccgtcctt
     3061 gtcggtgacg gtcagctcat ccagttcata gccgtcatcg ggcttgacag tgatggtgac
     3121 cgtggtgccc ttggaggcgc tcttggggga gacagtgacg tcgccgaggc ggctgctgtc
     3181 cacgctgacc gtgtaggtgg c
//
LOCUS       DBMDSJ010000183         3193 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7536_length_3193_cov_1.570181, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000183 DBMDSJ010000000
VERSION     DBMDSJ010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_3193_cov_1.570181"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2874)
                     /locus_tag="ACZPAQ_05380"
     CDS             complement(<1..2874)
                     /locus_tag="ACZPAQ_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR0903451.1"
                     /translation="MNREGYQQRGLYDPAFEHDACGIGAVVDIKGRQSYETVDSALKI
                     VEKLEHRAGKDAEGKTGDGVGILLQISHQFFQKAATECGIQLGEARDYGVGMFFFPQD
                     TLRRNQAKKMFEVIVAKEGMEFLGWRKVPVCPEVLGQKALSKMPNIQQAFVKRPGDAA
                     RGLEFDRRLYVARRVFEQSNDNTYVPSLSSRTVVYKGMFLVGELRKFYLDLQNRDYHS
                     AIACVHSRFSTNTNPSWERAHPNRLILHNGEINTIRGNADRMLAREETMSNPVIDDAL
                     DKIYPVVNGAGSDSAMLDNTLEFLVMSGMDLPLAVMVTIPEPWTKDGSISRAKRDLYQ
                     YYAIMMEPWDGPASILFSDGDAMGAVLDRNGLRPSRYYITDDGKLILSSEVGALDLDP
                     RKIVKKSRLEPGKMLLVDTVQGRIIDDAELKETYAARQPYGEWLDRGLIRLKDLPVPN
                     KKVPGYSQEELTRLEKAFGYTYEDVKNTILPMARTGAEPTAAMGVDIPLAVLSDHEQP
                     LFNYFKQLFAQVTNPPIDAIREEVVTDTTVYLGNDGNLLEERADNAHALQVGNPILTS
                     VDLMKIRDMKQPGFQVETVSILYYKNTPLKRALDHLFVAVDRAYRNGANIVILSDRGV
                     DENHVAIPSLLAVSAVERYLIRTKKCTAVSIVLESAEPRDVNHFATLLGYGARAVNPY
                     LAHAAIGSLIDQGLLDKDYHTAVKDYNQAILSGIVKIASKMGISTIQSYQSAQIFEAV
                     GIDRVVVDEYFTGTLSRVGGVGLKEIAQLVDDHHSRAFDPLGLGGDPTLDSRGEHKAR
                     AGQEDHMYSPQVIHLLQEATRRGDYKLFKEYTALVDDASKPHTLRGLMEMQYLDTPIP
                     LDEVESVDSIVKRFKTGAMSFGSISAEAHECMAVAMNTLGGKSNCGEGGEDPARFGTL
                     KNSAIKQVASGRFGVTSEYLVSAQDLQIKMAQGAKPGEGGHLPG"
BASE COUNT          558 a          984 c          972 g          679 t
ORIGIN      
        1 ccccggcagg tggccgccct cgccgggctt ggccccctga gccatcttga tctggagatc
       61 ttgggcggac accagatact cgctggtcac gccgaagcgg ccggaggcca cctgcttgat
      121 ggcggaattt ttcagggtgc cgaagcgggc ggggtcttcg ccgccctccc cgcagttgga
      181 tttgcccccc agggtgttca tggccaccgc catgcactcg tgggcctcag cggagatgga
      241 gccgaaggac atagccccgg tcttgaacct cttaacgatg gagtccacgc tctccacctc
      301 gtccaaggga atcggggtgt ccagatactg catctccatc aggccgcgga gggtgtgggg
      361 cttggaggcg tcgtccacca gggccgtgta ctccttgaag agtttgtagt cccccctgcg
      421 ggtggcctcc tggaggagat ggatgacctg ggggctgtac atgtggtcct cctggccagc
      481 gcgggccttg tgctcgcccc ggctgtccag ggtagggtcg ccgcccaggc ccagggggtc
      541 gaaggcccgg ctgtggtggt catccaccag ttgagcgatc tccttcagac ccacgccccc
      601 cacccggctg agggtaccag tgaaatactc gtccaccacc acccggtcaa tgcccactgc
      661 ctcgaagatc tgggcagact ggtaggactg gatggtagag atacccatct tggaggcgat
      721 cttcacgatg ccgctgagga tggcctggtt atagtccttc accgcggtgt ggtaatcctt
      781 gtccagcaaa ccttggtcaa tgagggagcc gatggccgca tgggccagat aggggttcac
      841 cgcccgggca ccgtagccca gcagcgtggc gaagtggttc acgtcccggg gttccgctga
      901 ctccagtacg atggacacgg cggtgcactt cttggtgcgg atcaggtatc gctccacggc
      961 tgagacagcc agcagggacg ggatggccac atggttctcg tccacccccc ggtcggagag
     1021 gatgacgatg ttggccccgt tgcggtaggc ccggtccacc gcaacgaaca ggtggtccag
     1081 cgcccgcttc agaggggtgt tcttgtagta taggatggag acggtctcca cctggaagcc
     1141 gggctgcttc atgtcccgga tcttcatcag gtctaccgag gtgaggatag ggttgcccac
     1201 ctgaagggcg tgagcgttgt ccgcccgctc ctccagcagg ttgccgtcgt tgcccagata
     1261 gacggtagtg tcggtgacca cctcctcccg gatggcgtcg atggggggat tggtcacctg
     1321 ggcgaagagc tgcttgaagt agttgaacag gggctgttcg tggtcggaga ggaccgccag
     1381 tgggatgtcc acccccatgg cggcggtggg ctccgccccg gtgcgtgcca tagggaggat
     1441 ggtgtttttc acatcctcat aggtataacc gaaggccttc tccagccggg tcagctcctc
     1501 ctgggagtag ccgggcacct ttttgttggg gaccggcagg tccttcagcc ggatgaggcc
     1561 ccggtccagc cactcgccgt agggctggcg ggcggcgtag gtctccttca gctccgcgtc
     1621 atcaatgata cgcccctgga ctgtatccac cagcagcatc ttccccggct ccagccggga
     1681 tttcttcacg atcttcctgg ggtccaggtc cagggcaccc acctcggagg agaggatcag
     1741 cttaccgtcg tcagtgatat agtaccggga ggggcgcagc ccgttccggt ccagcaccgc
     1801 tcccatggcg tcgccgtcgg agaacaggat ggaggccgga ccatcccagg gctccatcat
     1861 gatggcgtaa tactggtaca ggtcccgctt ggcccggctg atactgccgt ccttggtcca
     1921 gggctccggg atggtgacca tcaccgcgag gggcaggtcc atgccgctca tgaccaggaa
     1981 ctccagggtg ttgtccagca tggccgagtc ggacccggcc ccgttgacca cggggtagat
     2041 cttgtccagg gcgtcgtcga tcaccgggtt ggacatggtc tcctcccggg ccagcatccg
     2101 gtctgcgttg ccccggatgg tgttgatctc gccgttgtgg aggatcaggc ggttagggtg
     2161 ggcccgctcc caggagggat tggtgttggt ggagaagcgg gagtgaacgc atgcgatggc
     2221 gctgtggtag tcccgattct gcaggtccag gtagaacttc cgcagttccc ccaccaggaa
     2281 catccccttg tataccactg tccggctgga cagggagggg acataggtgt tgtcgttgga
     2341 ctgctcaaag actcggcggg ccacatacag cctgcggtcg aactccagtc cccgggccgc
     2401 atcccccggc cgcttcacga aggcctgctg gatgttgggc atcttggaca gtgccttctg
     2461 gcccagcacc tctgggcaga caggcacctt ccgccagccc agaaattcca tgccttcctt
     2521 ggccacaatg acctcgaaca tcttcttagc ctgattgcgc ctgagagtgt cctggggaaa
     2581 gaaaaacata cctacgccat agtctcgcgc ctcccccagc tggatgccgc actccgtggc
     2641 cgccttctga aaaaactggt gggagatctg gaggaggatg cccacgccgt caccggtctt
     2701 tccctcagcg tcctttcccg ctcgatgctc cagcttctcc acgattttca gcgcactgtc
     2761 caccgtctca tagctctggc ggcccttgat gtccaccacg gctccgatgc cgcaggcatc
     2821 gtgctcaaac gccgggtcat acaagccgcg ctgctgatag ccttctctgt tcatctcgat
     2881 ttcccctttc caggtcagct tacaaaaata cggcagtcaa actcccggac tgccgccgcg
     2941 agactgcgct gtctctccca tacgcacata gccgtctttc accgcagggc tcctgtccca
     3001 agcctagtca ttttgcatga acttcgcctc cttctttcaa gtttaggcat ctcgaaatta
     3061 tgtctctatc agagacatga ggccctctgt tttcagctat gggcgaaagt atagtacact
     3121 acttcgtgaa agtcaatatt ttttgaaatt atattttatc tttattgcaa atatatggcc
     3181 aactccagga gcg
//
LOCUS       DBMDSJ010000184         3185 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7556_length_3185_cov_1.885863, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000184 DBMDSJ010000000
VERSION     DBMDSJ010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7556_length_3185_cov_1.885863"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="ACZPAQ_05385"
     CDS             <1..663
                     /locus_tag="ACZPAQ_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903452.1"
                     /translation="AASAAITLIPGDVATPIADKLADLSSHFLLVLCAVFLEKYLLTI
                     TGTVTFSFLIPVSCLLYVLYVLFDWKSLRLLAARLTAFGLLIFLVIPASVYVSDLIED
                     SYQASIEATIETARDAAAELESESETAGEGEKGFFSGLISTVTDGVSGITDKVGEMVN
                     RFMEALAVMLVTCCAIPVLVLLSFLWFAKILLSVELSVSYSGIHHGLKDRLFRKKRGE
                     PQ"
     gene            760..1482
                     /locus_tag="ACZPAQ_05390"
     CDS             760..1482
                     /locus_tag="ACZPAQ_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0903453.1"
                     /translation="MQITAVFHRKGLRLATGVLLAMLLLAAGFFWYVSDYYRAEDAAL
                     DVLASGENIEVRGSLTILSPSYPTDTAIIFYPGAKVEGAAYLPLLDQLRRTGLTCILV
                     EMPFRMAIFDVNAAEDVMAQFPEIRHWYLAGHSMGGAMASQFAAGHRDQVDGLILLGA
                     YLYGEYPSADTLTVYGSLNQSVEDKIDYTEHVVEIDGGNHAQFGSYGLQRGDPPAEIT
                     PQEQQARTVEAISDFLAQRQER"
     gene            1567..>3185
                     /locus_tag="ACZPAQ_05395"
     CDS             1567..>3185
                     /locus_tag="ACZPAQ_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR0903454.1"
                     /translation="MQTSTDLRNLLARIDRKGYPAYKDTRGAYQFPAYVLSIDHVQGD
                     PFAAPSKLSIHVNGKQAGFPRQLYQTPCRRIALQDALTRQFGRRAAQASLQAKGSGHS
                     GLISISRCGQEVLERTACALDPEHGDLLLRLEVGFPANGRTINARELEKILFDLLPRC
                     VEESLLYRNLDGKRLQEAADLAEDQQDIRRQLPALGLCAFVADGSILPRTSGVSSLPM
                     KQAVPFRSPRELSVTLELPHRGTITGMGIPKGITLIVGGGYHGKSTLLKALELGVYDH
                     IAGDGREYVITDRTAVKIRAEDGRSIRNTDISMFINNLPNGKDTVHFTTEDASGSTSQ
                     AAGVVEAMEAGTSLLLMDEDTSATNFMIRDELMQRVIHRDMEPITPFLDRIRELYGIH
                     GISTVIVAGSSGAFFHAADRIIQMDRYVPRDITASAKEEARAFPAGSAVMEPAPAPGL
                     CRCPRPSPQFRGDRIKLKALGRDGISIDRETIDLRYVEQLADGEQSAALGWCMVYAQR
                     RLMDGRRTLRQIVDELDRLMEEQGLPALCGSP"
BASE COUNT          574 a         1068 c          960 g          583 t
ORIGIN      
        1 gccgcttctg cggcgatcac gctgatcccc ggtgacgtcg ccaccccgat cgccgacaag
       61 ctggccgatc tgagctccca ttttctgctg gtgctgtgtg ccgtgttcct ggaaaaatat
      121 ctgctgacca tcaccggaac ggtgaccttt tccttcctga tccccgtgtc ctgcctgctg
      181 tatgtgctgt atgtcctctt tgactggaaa agcctccgtc tgttggcggc aaggctgacc
      241 gcgtttggac tgctgatctt tttggtcatc ccagccagcg tctatgtctc tgatctgatc
      301 gaggactcct accaggcatc catcgaggcc accattgaaa cagccagaga tgccgccgca
      361 gagctggaga gcgaatcaga gacggcgggc gagggagaaa aaggcttctt ttccgggctg
      421 atctccaccg tgacagacgg ggtctccggg atcacggaca aagtgggcga gatggtcaac
      481 cgctttatgg aggcgctggc cgtgatgctg gtgacctgtt gcgccatccc ggtcctggtc
      541 ctgctgtcct tcctctggtt tgccaagatc ctgctgtccg tggagctgtc ggtcagttac
      601 agcggcatcc accacgggct gaaggatcgg cttttccgaa aaaaacgggg agagccccag
      661 taaaggcctc ccgcaggtcg ggtgtgcgga gatacttcgc acgagccccc gccagcaagg
      721 aaactgatcc gatcataaca gatgggaagt ggctctctta tgcagatcac agctgttttt
      781 caccggaagg ggctgcggct ggccacgggt gtcctgctgg cgatgcttct tctggcggcc
      841 ggatttttct ggtatgtatc cgactactac cgggcagagg atgcggccct ggatgtattg
      901 gccagcggcg agaacattga ggtccggggc agcctgacca tcctctcccc gtcctatccc
      961 acggacactg ccatcatctt ttaccccggg gccaaggtgg agggggctgc ctacctgcct
     1021 ctgctggacc agctgcggag gaccggcctc acctgtattc tggtggagat gcccttccgc
     1081 atggccatct ttgatgtcaa cgccgccgaa gacgtgatgg cccaatttcc ggagatccgg
     1141 cactggtatc tggccggaca ctccatgggc ggagccatgg cctcccaatt cgccgccggg
     1201 caccgggacc aggtggacgg cctcatcctg ctgggggcct atctctacgg cgaatacccg
     1261 tccgcagaca ccctcaccgt ctacggctcc ctgaatcaga gcgtggagga caagatcgac
     1321 tatacggaac atgtggtgga gatcgacggc ggcaaccatg cccagttcgg cagctacggc
     1381 ctccagcggg gcgacccgcc ggcggagatc acgcctcagg aacagcaggc ccggactgtg
     1441 gaggccatct ccgacttcct cgcccagcgg caggagagat aggcgctccc cccatccttt
     1501 cccacaggca cttcaggccc tgtgccgctc tccggggcac aaaagcaaat tgacaggagt
     1561 atcgctatgc agacttcaac agatctgaga aacctattgg cccgcatcga ccgcaagggc
     1621 tatccggcct ataaggacac ccggggcgcc tatcagttcc ccgcctacgt gctgtccatc
     1681 gaccacgtac agggggaccc ctttgccgct ccctccaaat tgagcatcca tgtgaacgga
     1741 aagcaggccg gattccccag gcagctctat cagaccccct gccggcgcat cgcccttcag
     1801 gatgcgctca cccgccagtt tggccggcgg gcggcccagg cttcccttca ggctaagggc
     1861 tcgggacaca gcggcctcat ctctatcagc cggtgcggcc aggaggtgct ggagcggacc
     1921 gcctgcgccc tggacccgga gcacggcgat ctgctgctgc ggctggaggt gggcttcccc
     1981 gccaacggtc gcaccatcaa cgcccgggag ctggaaaaga tcctcttcga cctgctgccc
     2041 cggtgcgtgg aggagtccct gctctaccgc aatctggacg gaaagcgcct ccaggaagcg
     2101 gctgacctgg cagaggacca gcaggatatc cgccggcagc tgcccgccct gggcctgtgc
     2161 gcctttgtgg ccgacggcag catcctgccc cggacctccg gcgtctcctc cctgcccatg
     2221 aagcaggccg tccccttccg ctcccccaga gagctctcgg tcaccctgga gctgccccac
     2281 cgggggacca tcaccggcat gggcatcccc aaggggatca ctctgatcgt gggcggcggc
     2341 taccacggga agtctaccct gctcaaggcc ctggagctgg gggtgtacga ccacatcgcc
     2401 ggggatgggc gggagtatgt catcaccgac cgcacagcgg tcaagatccg tgccgaggac
     2461 gggaggagca tccggaacac cgacatctcc atgtttatca acaacctgcc caatggaaag
     2521 gacactgtcc atttcaccac cgaggacgcc agcggcagca cctcccaggc cgccggcgtg
     2581 gtggaggcca tggaggcggg gacctccctg ctcctcatgg atgaggacac cagcgccacc
     2641 aacttcatga tccgggatga gctgatgcag cgggtcatcc accgggacat ggagcctatc
     2701 acccccttcc tcgaccgcat ccgggagctc tatggtatcc acggcatctc cactgtcatc
     2761 gtggcaggca gctccggcgc cttcttccac gccgccgacc gcatcatcca gatggaccgg
     2821 tatgtccccc gggacatcac cgcctccgcc aaggaggagg cccgggcctt ccccgccggg
     2881 agcgccgtca tggagcctgc tcccgccccc ggtctctgcc gctgtcccag accctccccc
     2941 cagttccggg gagaccggat caagctcaaa gccctgggcc gggacggcat atccatcgat
     3001 cgggagacca tcgatctgcg gtatgtggag cagctggctg acggcgagca gtctgctgcc
     3061 ctgggctggt gcatggtcta cgcccagcgg cgcctgatgg acggcaggcg caccctgcgc
     3121 cagatcgtgg acgagctgga ccggctgatg gaagaacagg gcctgcccgc cctgtgcggc
     3181 tcccc
//
LOCUS       DBMDSJ010000185         3148 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7643_length_3148_cov_1.665245, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000185 DBMDSJ010000000
VERSION     DBMDSJ010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7643_length_3148_cov_1.665245"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="ACZPAQ_05400"
     CDS             <1..472
                     /locus_tag="ACZPAQ_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903455.1"
                     /translation="SQGIQPLVSTAHGQDRPGLIRQYLRYALITLLVLSAALYLLLFL
                     FAGPVALIFNSEGDPRLQQIAEEGLRLYFTGALFAGFNIILSMYFTSVERALPAHITS
                     LLRGFLLILPMAFLLAACWGMTGVWLAFPLTEAVTAALGAGFYALYARRGRARS"
     gene            complement(557..946)
                     /locus_tag="ACZPAQ_05405"
     CDS             complement(557..946)
                     /locus_tag="ACZPAQ_05405"
                     /inference="COORDINATES: protein motif:HMM:NF025634.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HIR0903456.1"
                     /translation="MGEMKRLWAAVGILAALLAAALGTGWWVDGLTADYVSRLEQAQA
                     MSERGDWERAAQVTGGVYRNWQERSFWLHALLRHSDTDQVLLLFRSVEEYLKLEEMDQ
                     YAAANAQLIAQLELLAEMEDPSLENVL"
     gene            complement(950..1672)
                     /locus_tag="ACZPAQ_05410"
     CDS             complement(950..1672)
                     /locus_tag="ACZPAQ_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HIR0903457.1"
                     /translation="MERSARRAGGGQEEAMFTALIRTVILYILIIAGVRLMGKRQVGE
                     LEPSELVLSLLIADLASVPMQDLGIPLHAGVVPILALLSLTMILSVLTMKSIRFRTLM
                     CGRPSIVVRDGKVDQGEMRRNRLTVDELLEELRGQGYTDLAGVKYGILETNGRLSVLP
                     YAEQRPATARQLGLESRETGLPVVIISDGRILQDNLKRRGLDLSWLDKQLAEHGYPDF
                     RDVFLLTVDEAGGVYFVPREGK"
     gene            1888..2970
                     /locus_tag="ACZPAQ_05415"
     CDS             1888..2970
                     /locus_tag="ACZPAQ_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR0903458.1"
                     /translation="MKATLILANGSVFSGTSIGGTSDRICEMVFNTSMTGYQEILTDP
                     SYAGQGVVMSYPLIGNYGVNHEDNESRRPWVEALVVRHLSPQGSNFRCEDTLDSYLKQ
                     YDITGIQGVDTRALTKILRSQGSMNGMLTCAEHFNVAEVMERLRSYEVKDTIRRVTRT
                     EPEVFDAYGEETHLVAVMDYGVKQNIIECLRRRGCKVVALPAYTPASEVLSSGFDGVM
                     LSNGPGDPAENTEIIAELRKLYDSTIPIFGVCMGHQLLALATGAKTGRLAYGHRGGNH
                     PVRDLEKERVFITSQNHGYMVLSDTVAPAVAEVSHINVNDGTCEGLKYKRPNCFTTQF
                     HPEANAGPKDTEYLFNRFIDSMGGAK"
     gene            2970..>3148
                     /locus_tag="ACZPAQ_05420"
     CDS             2970..>3148
                     /locus_tag="ACZPAQ_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase preATP-grasp
                     domain-containing protein"
                     /protein_id="HIR0903459.1"
                     /translation="MPKYTDIKKVLVLGSGPIVIGQAAEFDYAGTQACRALKEEGLEV
                     VLINSNPATIMTDKD"
BASE COUNT          577 a         1077 c          892 g          602 t
ORIGIN      
        1 ctctcagggc atccagcctc tggtgagcac cgcccacggc caggaccgtc ccggtctgat
       61 ccgccagtac ctgcgctatg ccctgatcac cctcctggtc ctgtccgccg ccctctacct
      121 cctgctgttc ctctttgccg gaccggtggc cctgatcttc aacagtgagg gggacccccg
      181 gctccagcag atcgccgaag agggcctgcg gctctatttc accggcgcgc tctttgccgg
      241 gttcaacatc atcctgtcaa tgtatttcac ctcggtggag cgggccctgc ctgcccatat
      301 cacctccctg ctgcggggtt tcctcctgat cctccccatg gccttcctgc tggccgcctg
      361 ctggggcatg acgggcgtct ggctggcctt tcccctcacc gaagccgtca ccgcggccct
      421 gggcgctgga ttttacgccc tctatgcccg caggggcagg gcacggtcgt aaaaatcgtg
      481 agaagcgccc ccttgtcttc acgtcatgca aagagaccgg cccggacatg gatgccatgt
      541 ccgggccggt cctctcttac agcacatttt ccagagaggg gtcctccatc tccgccagca
      601 gctccagctg ggcgatgagc tgggcgttgg cggctgcgta ctggtccatc tcttccagct
      661 tcaggtactc ttccaccgag cggaacagga gcagcacctg gtccgtgtcg ctgtgtcgca
      721 gcagggcatg gagccagaag ctcctctcct gccagttccg gtacacccct cccgtcacct
      781 gggcagcccg ctcccagtcg cccctctcac tcatggcctg ggcctgctcc agccgggaaa
      841 cataatctgc agtcagtccg tccacccacc agccggtacc cagggcggcg gccagcagag
      901 cggccaggat gcccaccgcc gcccaaaggc gcttcatctc tcccattccc tactttccct
      961 ccctggggac gaaatacacg cccccggcct cgtccactgt cagcaggaac acgtcccgga
     1021 agtcgggata cccgtgctcc gccagctgct tgtccagcca ggacaggtcc agcccccgcc
     1081 gcttcaggtt gtcctgcaaa atgcgcccgt cgctgatgat gaccacaggc agcccggtct
     1141 cccggctctc cagccccagc tgccgggcgg tggccggcct ctgctcggcg tagggcagga
     1201 cggacagccg gccgttggtc tccaggatgc cgtatttcac cccagccaga tcggtgtagc
     1261 cctgcccccg cagctcctcc agaagctcat ccaccgtcag ccggttgcgg cgcatctccc
     1321 cctggtccac ttttccgtcc cggaccacga tgctgggccg gccgcacatc agggtgcgga
     1381 agcggatgct cttcatggtc agcaccgaga ggatcatggt cagggacagc agggccagga
     1441 tgggcaccac cccggcatgg agggggatgc ccaggtcctg catgggcacc gaggccaggt
     1501 ccgcgatgag cagggagagc accagctccg agggctccag ctcccccacc tggcgcttgc
     1561 ccatcagccg caccccggcg atgatgagga tgtataggat cacggtacgg atcagcgcgg
     1621 tgaacatggc ttcctcctgt ccgcccccgg cccgccgggc gcttcgttcc atccctctca
     1681 ttcttgccct tctccggccc tattattctt taattcttcg gtgcgttttc tccaaaagcc
     1741 gccggtgttt tttttcgaaa aaaggagaaa cgacatcttg cattgcgggt cgttttccga
     1801 taaaatatta agaagcaaac tggagggtca tgccctcctg aagcaatgcg tatgagaacc
     1861 tggaacggaa ggcgtgtgag agaattgatg aaagcgactt tgatcctggc caacggaagc
     1921 gttttttccg ggaccagcat cggcggcacc tccgaccgga tctgtgagat ggtgttcaac
     1981 acctccatga ccggctatca ggagatcctg accgacccct cctatgctgg acagggcgtg
     2041 gtgatgtcct atcccctcat tggcaattac ggcgtcaacc acgaggacaa cgagtcccgc
     2101 cgcccttggg tggaggccct tgttgtccgc catctgtctc cccaggggag taatttccgc
     2161 tgtgaggaca cactggacag ctatctgaaa caatacgaca ttaccggcat ccagggcgtg
     2221 gatacccggg cactgacgaa aatactccgc agtcagggga gcatgaacgg tatgctcacc
     2281 tgtgcggagc attttaatgt ggccgaggtc atggagcggc tgcgctccta tgaggtgaaa
     2341 gataccatcc gcagggtgac ccggaccgag cccgaggtgt ttgacgccta tggcgaggaa
     2401 acgcacctgg tggccgtgat ggactacggc gtgaagcaga acatcatcga gtgcctgcgc
     2461 cgccgcggct gtaaggtcgt ggccctgccc gcctacaccc ccgccagcga ggtgctctcc
     2521 agcggctttg acggcgtgat gctctccaac ggccccggcg atcccgctga gaacactgag
     2581 atcatcgcag agctgcgcaa gctctatgac agcaccatcc ccatcttcgg cgtgtgcatg
     2641 ggccaccagc ttctggccct ggccaccggc gccaagacag gccggctggc ctatggccac
     2701 cggggcggca accaccccgt ccgtgacctg gagaaggaac gtgtgttcat caccagccag
     2761 aaccacggct atatggtcct ctctgacacg gtggcccctg ccgtggccga ggtatcccac
     2821 atcaacgtca acgacggcac ctgcgaggga ctgaagtaca agcgccccaa ctgctttacc
     2881 actcagttcc atcccgaggc caacgccggc cccaaggata cggaatacct cttcaaccgc
     2941 tttatcgact ccatgggagg tgccaagtaa tgccgaagta caccgatatc aagaaggtcc
     3001 tggtgctggg ttccggcccc atcgtcatcg gccaggccgc cgaattcgac tatgccggca
     3061 cccaggcctg ccgcgcgctg aaggaggagg gcctggaggt cgtcctgatc aactccaacc
     3121 ccgccaccat tatgacggac aaggacat
//
LOCUS       DBMDSJ010000186         3148 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7644_length_3148_cov_1.651132, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000186 DBMDSJ010000000
VERSION     DBMDSJ010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7644_length_3148_cov_1.651132"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="ACZPAQ_05425"
     CDS             <1..1119
                     /locus_tag="ACZPAQ_05425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903460.1"
                     /translation="YNRQAWRHGVLGMLAARELELPVQKRLSRSAGLVSLSCLLLNCV
                     TAGLLIWCSSWSWGSVRLETAWLLCLPPLLLAAVTAAFLFRQRRLWGDLGALTGQIAA
                     VRAGELARPLELPRDHDLSQAADDLNHIQQGLQAAMEERTRSERMKVELVTNVSHDLK
                     TPLTSIISYAELLEQEHLEPPAGEYVTILSQKAQRLKGMVQDVFEVSKAASGQLPVSL
                     KRLDLARLLRQTLADMDQPIQESGLQLRTQLPDHSVLIQGDSDRLYRVFQNLLGNALK
                     YSLAGSRVYLTLTVEEGQAVAALRNTSAAELRPGLDYTQRFVRGDESRTDGGSGLGLS
                     IASSFVQACGGQFQVETQADLFTALVSFPLDASPSDPR"
     gene            complement(1212..2606)
                     /locus_tag="ACZPAQ_05430"
     CDS             complement(1212..2606)
                     /locus_tag="ACZPAQ_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903461.1"
                     /translation="MTRDLTTGSPMRLIAGFTLPTLLGLLFQQLYNLVDTMIVGKLLG
                     SAALAAVGSTGSISFLVIGFCMGICNGFAIPVAQRIGAGEPSKMRRYVANAAYLGAGI
                     AVVLTAATGLLCRTILTAMQTPEEIFADAYSYIFVIFMGIPASILYNLLAAIMRSLGD
                     SKTPVYFLALASILNIGLDFAFILLFRAGVAGAAIATVLSQALSGLACLAFMVRRFPI
                     LRMQGAEKRFDLHSAKVLCWMGIPMGLQYSITAVGSIILQSAVNGLGTAAIASVAAGA
                     KLFQLICCPYDAMGSSMATYCGQNVGACKLDRLGQGMRACAWLGLGYAVLAVGAMLLF
                     APQCAMLFLNQDEADLARLVSLTSQYITTLTAFFFPLALVNIVRFSIQGMGFSQLAIL
                     AGVLEMAARTVMGQVFVPLFGFTAACFASPVAWISADLFLIPAAILCIRHLRKLYPDQ
                     TEEAALRPVRAEGP"
     gene            complement(2798..>3148)
                     /locus_tag="ACZPAQ_05435"
     CDS             complement(2798..>3148)
                     /locus_tag="ACZPAQ_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903462.1"
                     /translation="PGVLARIFTPEQELIQLVSRVMPLFFGGIWAFGAQMACQSTFMA
                     LGQARTSLFLALLRKVILLVPLALILPRVTGSVMGIYAAEPIADILASATTLTLFLSQ
                     RKKLLPTEKDAPKG"
BASE COUNT          581 a         1004 c         1061 g          502 t
ORIGIN      
        1 tacaacaggc aggcatggcg gcacggagtg ctggggatgc tggccgcccg ggagctggag
       61 ctccctgtcc agaagcggct cagccgctcc gccggcctgg tctccctgtc ctgtctgctg
      121 ctgaactgcg tcacagccgg tctgctgatc tggtgctcca gctggtcctg gggctccgtc
      181 cggctggaga cggcctggct cctctgtctg ccgccccttc tgctcgctgc tgtgacagcg
      241 gcattcctgt tccgccagcg ccggctgtgg ggggatctgg gggcgctcac cggtcagatc
      301 gccgccgtcc gggcggggga gctggcccgc cccctggaac tgccccggga ccacgacctg
      361 tcccaggcgg cggatgacct gaaccacatc cagcaggggc tccaggccgc catggaggag
      421 cgcacccgca gcgagcggat gaaggtggag ctggtcacca atgtgtccca tgacctgaag
      481 acccccctca cctctattat cagttatgcc gagctgctgg agcaggaaca tctggagccc
      541 cctgccgggg agtatgtgac catcctctcc caaaaggccc agcggctgaa ggggatggtg
      601 caggatgtgt tcgaggtgag caaggcggcc tccggccagc tgccggtgag cctcaaacgg
      661 ctggacctgg cccgcctgct ccgccagacc ctggcggata tggaccagcc catccaggag
      721 agcgggctgc agctgcgcac ccagctcccc gaccactcgg tgctcatcca gggggacagt
      781 gaccgcctct accgggtgtt ccagaatctg ctgggcaacg ccttgaaata ctccctggcc
      841 ggttcccggg tgtacctgac cctcacggtg gaggagggac aggcggtggc cgccctgcgc
      901 aacacctctg ccgcagaact ccgtcccggc ctggactaca cccagcgctt cgtccggggg
      961 gacgagagcc gtaccgacgg cggcagcggg ctgggcctgt ccattgcctc cagctttgtc
     1021 caggcctgcg gcggtcagtt ccaggtagag acccaggccg acctcttcac cgccctggtc
     1081 tccttcccct tggacgcctc cccgtccgat cccaggtgat ccgcatttcg gaaacagccg
     1141 tacgggcgga cttttttgac aaatggaaag gcccaggcgc ttttgcgcct gggccctctg
     1201 tgtcaatggg gttacggtcc ttccgcgcgg acggggcgga gggcggcctc ctcggtctga
     1261 tcgggataca gcttccgcag atggcggatg cagaggatgg cggcggggat caggaacaga
     1321 tcggcgctga tccaggccac cggggaggca aagcaggcgg cggtgaagcc gaacaggggg
     1381 acgaagacct ggcccatcac cgtccgggcg gccatctcga gcacccccgc caggatggcc
     1441 agctgactga agcccatccc ctggatggag aagcgcacga tattgaccag ggccaggggg
     1501 aagaagaaag cggtgagggt agtgatatac tggctggtga gggacacaag ccgggcaaga
     1561 tcggcctcat cctggttcag gaacagcatg gcgcactggg gagcgaagag cagcatggcc
     1621 cccacggcca gcacggcata gcccagcccc agccaggcgc aggcccgcat cccctgtccc
     1681 agccggtcca gcttgcaggc ccccacgttc tggccgcagt aggtggccat agaggagccc
     1741 atggcgtcat aggggcagca gatcagctgg aacagcttgg ctccggcggc caccgaggcg
     1801 atagcggcag ttcccaggcc gttgacggcg gactggagga tgatggagcc gacggcggtg
     1861 atggagtatt ggaggcccat ggggatgccc atccagcaca gaaccttggc ggagtggagg
     1921 tcaaaccgct tctccgcccc ctgcatgcgc aggatgggga agcgcctgac catgaaagcc
     1981 agacaggcca ggccggacag agcctgggag agcacggtgg cgatggccgc ccccgccact
     2041 cccgcacgga agagcagaat gaaagcgaaa tccaggccga tattcaggat ggaggccagg
     2101 gccaggaaat agaccggcgt cttgctgtcc cccagagagc gcatgatggc agccagcagg
     2161 ttgtagagga tggaggccgg gatacccatg aagatcacga agatataact gtacgcatcc
     2221 gcgaagatct cctccggcgt ctgcatggcg gtcaggatcg tacggcacag caggccggtg
     2281 gctgcggtga gcaccacggc gatgcccgcc cccagatagg cggcgttggc tacgtatcgg
     2341 cgcattttgg agggctctcc cgccccaatg cgctgagcta ccgggatggc gaaaccgttg
     2401 cagatgccca tgcagaagcc gatgaccaga aaactgatgg agccggtgga gcccaccgcc
     2461 gccagcgcgg cagaccccag cagtttgccc acgatcatgg tatccaccag attgtagagc
     2521 tgctggaaca acaggcccag cagcgtgggc agggtgaagc cggcgatcag ccgcatgggg
     2581 ctgccggtgg tgagatcgcg ggtcataggg ggtccctcct tctgatgcgg agcggggcgc
     2641 ctgcgcccgt ctcctggact gcaatattat agcggtccgc ccccctttgg gatagagctt
     2701 tttttcacaa tcttttcccc gctcccctcc ggccggtctg tcaaattgtc atgaccggga
     2761 ccgtggggcc ttctgccaca gcggcccgtc tcgcccctca gcccttcgga gcgtccttct
     2821 cggtgggcag cagctttttc cgctgagaga ggaacaaagt cagggtggtg gcactggcca
     2881 ggatgtcggc gataggctcg gccgcataga tccccatgac gctgccggtg actctgggca
     2941 ggatgagggc caggggcacc agcaggatca ccttccgcag cagggccagg aacaggctgg
     3001 tcctggcctg accaagggcc atgaaggtgc tctggcaggc catctgggcg ccgaaggccc
     3061 agatgccccc gaagaagagg ggcatgaccc gggacaccag ctggatcagc tcctgctcag
     3121 gggtgaagat acgggccagc acccccgg
//
LOCUS       DBMDSJ010000187         3147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7647_length_3147_cov_1.806960, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000187 DBMDSJ010000000
VERSION     DBMDSJ010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7647_length_3147_cov_1.806960"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="ACZPAQ_05440"
     CDS             complement(<1..161)
                     /locus_tag="ACZPAQ_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HIR0903463.1"
                     /translation="MSKRIAILVSGGGTNLQALIDAQGRGELVNGRISAVISSTPDAF
                     ALERAKNAG"
     gene            complement(309..1352)
                     /gene="purM"
                     /locus_tag="ACZPAQ_05445"
     CDS             complement(309..1352)
                     /gene="purM"
                     /locus_tag="ACZPAQ_05445"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR0903464.1"
                     /translation="MKNSHSESYAAAGVDVTAGYEAVERIKPMVESTYIPGVMGTLGG
                     FGGMFAPDLTGMKKPVLVSGTDGVGTKLKLAFLMDKHDTVGIDCVAMCVNDIICGGAA
                     PLFFLDYIACGKNYPERIADIVSGITEGCRQAECALVGGETAEMPGFYPEEEYDLAGF
                     SVGLVDEEKIIDGRRLSEADVLIGLASSGVHSNGFSLVRKVFDVEHADLKTPREDLGG
                     KSLGEALLAPTRIYVRSVKALLRAGVDIHAVSHITGGGFYENVPRMMTQGLTAQIDLS
                     TFPRLPIFDLIQKTGDIPERDMYNTYNMGIGMILALPAEQAEQALSVLKGAGETAYQI
                     GRVIPGEAGVELV"
     gene            complement(1568..2992)
                     /locus_tag="ACZPAQ_05450"
     CDS             complement(1568..2992)
                     /locus_tag="ACZPAQ_05450"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR0903465.1"
                     /translation="MGGFFGAVSHQDVTLDIFFGVDYHSHLGTRRGGMIIHDAKDGFQ
                     RQIHSIENTPFRTKFEKDLSDFHGCSGIGCISDTDPQPLLVRSHLGLYAITTVGIINN
                     AEELIQRYFSDHGHQFMAMSSGKVNSTELAAALINQKDDLVSGILHAQEEIDGSLTLL
                     LLTEQGEIIAARDRLGRLPVLVGQNDSGCCVSFESFAYHKLDYHDAYELGPREIVRIT
                     ADGFETLSPAGQEMKICGFLWTYYGYPNSNYEGINVEVMRYRNGEIMARDEVARGQLP
                     KVDYVAGVPDSGVPHAIGFANRSGKPFARPFVKYTPTWPRSFMPTNQDVRKQVAKMKQ
                     IPVPELIEGKKLLFVDDSIVRGTQLRETVEFLYESGAEEVHMRSACPPIMHSCKYLNF
                     SRGNSDMDLLARRTIQELEGDEGQQHLDEYADVHTERGQCMLKAICEKLGFDSLGYQS
                     LEGLLESIGLDRDKVCTYCWNGRE"
BASE COUNT          604 a          946 c         1009 g          588 t
ORIGIN      
        1 atgccggcgt tcttggcacg ctccagggcg aaggcgtccg gagtggagga gatgacggcg
       61 gagatccggc cattcaccag ctctccccgc ccctgggcgt cgatcagggc ctgcagattg
      121 gtcccgccgc cggagaccaa gatggcgata cgtttggaca tagcgaacct ccgagtgagt
      181 taggaattag aaagttaggg attaagaatt tggaatggat ggagtgcgcc tggggcgcac
      241 ggtttgaaat cggcccggcg gagccggaga cacaattccc aactcctcat ttctcattcc
      301 taatgtgatc agaccagctc cacgccggcc tcgccgggga tgacgcggcc gatttgatag
      361 gcggtctcac cggccccctt cagaacggac agagcctgct cggcctgctc tgcgggcagg
      421 gccaggatca tgccgatgcc catattgtag gtgttgtaca tgtcccgctc cgggatgtcc
      481 ccggtcttct ggatcagatc gaagatgggc aggcggggga aggtgctcag gtcgatctgg
      541 gcggtgagcc cctgggtcat catccggggc acgttctcat agaagccgcc gccggtgatg
      601 tgactgacgg catggatgtc caccccggcc ctcagcaggg ccttcaccga tcttacatag
      661 atgcgggtgg gggccagcag ggcctcgccc aggctcttgc cgcccagatc ctcacggggc
      721 gtcttcaggt cggcgtgctc cacgtcaaac accttccgca caagggagaa gccgttggag
      781 tggaccccag aggaggccag gccgatgagc acatccgcct cgctcaggcg tctgccgtca
      841 atgatctttt cctcgtctac cagcccgacg gagaagccgg ccaggtcgta ctcctcctcg
      901 gggtagaagc cgggcatctc tgcggtctcg ccacccacca gggcacactc cgcctggcgg
      961 cagccctcgg tgatgccgga gacgatgtcc gcgatgcgct cggggtagtt cttgccgcag
     1021 gcgatgtagt ccaggaagaa gaggggggca gcgccgccgc agatgatatc gttgacacac
     1081 atggccacgc agtcgatgcc cacggtgtca tgcttatcca tcaggaaggc cagcttcagc
     1141 ttggtaccca cgccatcggt gccggacacc agcacaggct tcttcatgcc ggtcagatca
     1201 ggggcaaaca tgccgccgaa gccgcccagg gtgcccatca ccccggggat ataggtggac
     1261 tccaccatgg gcttgatgcg ctccaccgcc tcatatccgg cggtgacgtc caccccggcg
     1321 gcggcgtagg actccgaatg ggagtttttc atcttaaaaa cctccaaatc actgtgttaa
     1381 agcctcgcag actttccgcc gaaagcggaa aggacaacaa agagtccgca ggcgaatcca
     1441 tttcgccgca ggacatcatg acccctggcc gtggcggcca gggccgcctt tcctgtggaa
     1501 aggcgagggg atatcgacga ggaagcgacg ttgcatccag gggggacatc gcccccctgg
     1561 aggggcctta ttccctgccg ttccagcagt aggtgcaaac cttgtcccgg tccaggccga
     1621 tggactccag cagtccctcc agggactggt agcccaggga gtcgaagccc agcttctcgc
     1681 agatggcctt cagcatgcac tggccccgct cagtatgtac gtcggcgtac tcatccagat
     1741 gctgctgccc ctcatccccc tccagctcct ggatggtgcg gcgggccagc aggtccatgt
     1801 cggagttgcc ccgggagaag ttcagatact tgcagctgtg catgatgggg gggcaggcgg
     1861 agcgcatatg gacctcctcc gcgccggact catagaggaa ttccacggtc tcccgcagct
     1921 gggtgccccg gacgatggag tcgtccacaa agaggagctt tttgccctcg atgagctcag
     1981 gcaccgggat ctgcttcatc ttggccacct gcttgcggac atcctgattg gtgggcatga
     2041 aggagcgggg ccaggtcggg gtgtacttga cgaaggggcg ggcaaagggc ttgccgctgc
     2101 ggttggcaaa gccgatggcg tgggggacgc cggagtcagg caccccggcc acatagtcca
     2161 ccttgggcag ctggccccgg gccacctcgt cccgggccat gatctccccg ttgcggtagc
     2221 gcatgacctc tacattgatc ccttcgtagt tggagttggg atagccgtag taggtccaca
     2281 ggaagccgca gatcttcatc tcctggccgg cgggggagag ggtctcgaag ccgtcagcgg
     2341 tgatgcggac gatctcacgg gggcccagct cgtaggcgtc gtggtagtcc agcttgtggt
     2401 aggcaaagga ctcgaaggag acacagcagc cgctgtcgtt ctgccccacc aggaccggca
     2461 gacggccgag ccggtcccgg gcggcgatga tctcaccctg ctctgtaagg agcagcaggg
     2521 tgagggagcc atcgatctcc tcctgggcgt gcaggatgcc ggagaccagg tcatccttct
     2581 ggttgatgag ggcagcggcc agctcggtgg agttgacctt tccggagctc atggccataa
     2641 actgatggcc gtggtcggaa aaataccgct ggatcagctc ctcggcgttg ttgatgatgc
     2701 ccaccgtggt gatggcgtac agtcccaggt gggaccgtac cagcaggggc tgtgggtcag
     2761 tgtcgctgat gcagccgatg ccgctgcacc cgtggaaatc ggacagatcc ttctcaaact
     2821 tggtgcggaa gggggtgttc tcaatggagt ggatctgccg ctggaaccca tctttggcgt
     2881 catggatgat catgccgccc cgccgggtgc ccaggtggga gtgatagtcc actccaaaga
     2941 agatatccag ggtcacatcc tggtgtgaaa ctgcgccaaa aaagccgccc atgtggtcct
     3001 cctaaaaagt ttagagtgaa gagttgcgcg tggagaaaag ggaaagagaa gaacatactc
     3061 cctcgctccc caccccctgc gcctgcccag ggagatctat ttcctgtgtt tgcggatcaa
     3121 aagccaggcc agggtccccg cacccag
//
LOCUS       DBMDSJ010000188         3146 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7649_length_3146_cov_1.371757, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000188 DBMDSJ010000000
VERSION     DBMDSJ010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_3146_cov_1.371757"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            124..690
                     /locus_tag="ACZPAQ_05455"
     CDS             124..690
                     /locus_tag="ACZPAQ_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR0903466.1"
                     /translation="MSQITKRALASALKVLLEHKPLNKITIADITEQCGVNRQTFYYH
                     FQDIYALLEWIYTTDAQRLLEGKRDGDTWQQGFLQVLEYIRDNRALVRNTYHSVSREL
                     LEGYLYRVTYQLMRDVVEERAAGTQVPEEDRAFLAHFYKYAFVGLTVEWMQGGMKEEP
                     ARLVSRVNTVMEGNIAAALERMARRKEP"
     gene            809..2581
                     /locus_tag="ACZPAQ_05460"
     CDS             809..2581
                     /locus_tag="ACZPAQ_05460"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR0903467.1"
                     /translation="MYYSSGNYEAFARPRKPEGVERKSAWLVGSGLASLAAACFLVRD
                     GQMKGENIHILEELKLPGGACDGIQDPTRGFVIRGGREMENHFECLWDLFRSIPSLEH
                     PENSVLDEYYWLNKEDPNYSLCRATVDRGKDAHTDGKFTLSDKAALEIMKLFLTRDED
                     LYDRTITDVFTEEFFRSNFWLYWRTMFAFETWHSALEMKLYLQRFIHHIGGLPDFSAL
                     KFTQYNQYESLILPMVHYLKEHNVQFQYDTQVTNVIFDIAPGRKVAKQLVWVREGREE
                     VRELTEDDLVFVTNGSCTENSSLGSNDRAPEFLIEPVGGCWQLWRNIAAQDPAFGRPD
                     KYCTKPGLTNWESATVTTLDDKIPPYLEKVCKRDPFSGKVVTGGIVTVKDSAWMMSYT
                     FNRQPHFKAQPEGQLVGWIYGLFTDVPGDYVKKPMRACTGREICMEWLYHLGVPEEEI
                     PLLAEQSASTIPCMMPYITAFFMPRTRGDRPDVVPEGCVNFAFIGQFAETARDTIFTT
                     EYSVRTGMEAVYTLLDIDRGVPEVWGSCYDVRCLLDAASKMMDGKKLTEMDLPFLYGF
                     VEKKALKHVSGTVIEELLLRYHLI"
     gene            2711..3091
                     /locus_tag="ACZPAQ_05465"
     CDS             2711..3091
                     /locus_tag="ACZPAQ_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0903468.1"
                     /translation="MHGTLSDQSLIYLQIAQMLEDDILRGVYREEEQVPSTNELSRVY
                     HINPATAAKGLNLLSAEGILYKRRGIGMFVAPGAEQAVRAKRKAAFYDSYVRPLVKEG
                     ASLAVTGEELLAMVRRAIEEGEAK"
BASE COUNT          645 a          920 c         1022 g          559 t
ORIGIN      
        1 agaagggcga tcaccaggag ctgctggacc agaagggacg ttactatcaa ctctacaccg
       61 gtcagttcca gctgtcctga cccccggacg tgggcggaag gcaccggaga caggaggagc
      121 gccatgtcgc agatcacaaa gcgggccctg gcctctgcgc tgaaggtgct gctggagcac
      181 aagccgctga ataagatcac catcgccgac atcaccgagc agtgcggggt gaaccgccag
      241 accttttact accactttca ggatatctac gccctgctgg agtggatcta caccaccgac
      301 gcccagcgcc tgctggaggg aaagcgggac ggggacacct ggcagcaggg gttcctccag
      361 gtcctggagt acatccggga caaccgggcc ctggtccgca atacctacca ctccgtcagc
      421 cgggagctgc tggagggcta cctgtaccgg gtgacctacc agctgatgcg ggacgtagtg
      481 gaggagaggg cggcggggac ccaggtcccg gaggaggacc gggccttcct ggcccacttc
      541 tacaagtacg cctttgtggg gctgacggtg gagtggatgc aggggggcat gaaggaggag
      601 cccgccaggc tggtgagccg ggtcaatacc gtgatggagg gaaatatcgc cgcggccctg
      661 gagcgcatgg cccggcggaa agagccctga gatccagaca aagggggccc gctgtcgcaa
      721 tttgcgacag cgggccccct ctatccattg gcaggcaggg ccgggggcgc tatactgacc
      781 tcacaaactc gatctggagg tcatggatat gtattacagc agcggaaatt acgaggcatt
      841 tgcccgcccc cgtaagccgg agggcgtgga acggaaatcc gcctggctgg tgggttccgg
      901 cctggccagc ttggcggccg cctgcttcct ggtgcgggac gggcagatga agggggagaa
      961 catccacatc ctggaggagc taaagctccc cggcggggcc tgcgacggca tccaggaccc
     1021 cacccggggc ttcgtgatcc ggggcggccg ggagatggag aatcacttcg agtgcctgtg
     1081 ggacctgttt cgctccatcc catccctgga gcacccggag aattctgttt tggacgaata
     1141 ctactggctg aacaaggagg accccaacta ttccctgtgc cgggccacgg tggatcgggg
     1201 aaaggacgcc catacagacg ggaagttcac tctgagcgac aaggccgccc tggagatcat
     1261 gaagcttttc ctcacccggg acgaggacct gtacgacagg accatcaccg acgtgttcac
     1321 cgaggagttc ttccgctcca acttctggct ctactggcgg accatgttcg ccttcgagac
     1381 ttggcactct gccctggaga tgaagctgta tctccagcgg ttcatccacc atatcggcgg
     1441 cctgcccgac ttctcggccc tgaaatttac ccagtacaac cagtatgagt ccttgatcct
     1501 gcccatggtg cactacctga aggagcacaa tgtgcagttt cagtatgaca cgcaggtcac
     1561 caacgtgatc ttcgacatcg ccccgggccg aaaggtggca aagcagctgg tgtgggtccg
     1621 ggagggccgg gaggaggtca gggagctcac tgaggacgac ctggtgttcg tcacaaacgg
     1681 ctcctgtacg gagaactcct ctctgggcag caacgaccgc gccccggagt tcctcattga
     1741 gccggtgggg ggctgctggc agctgtggcg gaacatcgct gcccaggacc cggccttcgg
     1801 ccgcccggac aagtactgca ccaagccggg ccttaccaac tgggagtcgg ccaccgtcac
     1861 caccctggac gacaagatcc cgccctatct ggaaaaggta tgcaagcggg accccttctc
     1921 cgggaaggtg gtcaccggcg gtatcgtcac ggtgaaggac tccgcctgga tgatgagcta
     1981 taccttcaac cgccagcccc acttcaaggc ccagccggag ggacagctgg tggggtggat
     2041 ctacggcctg ttcaccgatg tccccgggga ctatgtgaaa aagcccatgc gggcgtgtac
     2101 cggccgggag atctgcatgg agtggctgta ccacctgggg gtgccggagg aggagatccc
     2161 cctgctggcg gagcagtctg ccagcaccat cccctgcatg atgccctaca tcaccgcctt
     2221 cttcatgccc cggacacggg gggaccggcc cgacgtggtg ccggagggct gtgtgaactt
     2281 cgcctttatc ggccagttcg ccgagacggc acgggacacc atcttcacca ccgagtactc
     2341 cgtccgaacc ggtatggagg ccgtctacac cctgctggac atcgaccggg gggtgcctga
     2401 ggtgtggggg tcttgctacg atgtgcgctg tctgctggat gccgcatcca aaatgatgga
     2461 cggcaaaaag ctgacggaga tggacttgcc cttcctgtat ggattcgtgg agaagaaggc
     2521 cctgaagcat gtgtccggga ccgtgataga ggaactcctc ctccgctacc acctgatttg
     2581 atggctcccg ccccagcctg gtgctggggc gggacttttt ttgccttttt tattgacaaa
     2641 aagggatata ggtgatatgg ttaattacac aagtaatgaa ccaataaacc gaaggacagg
     2701 aggcggtgag atgcatggga ccctcagcga tcagtcactg atctatctcc agatcgctca
     2761 gatgctggag gacgacatcc tgcggggcgt ctaccgggag gaggagcagg tgccctccac
     2821 caatgagctc tcccgggtct atcacatcaa cccggccacg gcggccaaag ggctcaacct
     2881 cctctcggcg gaggggatcc tgtataagcg ccggggcatc ggcatgttcg tggccccggg
     2941 ggcggagcag gccgtgcggg ccaagcggaa agcggcgttt tacgacagct atgtgcggcc
     3001 cctggtaaag gagggggcct ccctggctgt gaccggggaa gaactgctgg ccatggtgcg
     3061 ccgggccatc gaggaaggag aagccaaatg aatacaggca ttttgaaagc agaggggctg
     3121 tgcaagtcct atgggagcaa gcgggt
//
LOCUS       DBMDSJ010000189         3131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7693_length_3131_cov_1.652805, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000189 DBMDSJ010000000
VERSION     DBMDSJ010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7693_length_3131_cov_1.652805"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="ACZPAQ_05470"
     CDS             complement(<1..237)
                     /locus_tag="ACZPAQ_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024076.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HIR0903469.1"
                     /translation="MSKREEQGKQGEPSLEELGHGVGSPFWGELHSFLRESYGVSPTV
                     EFSGCSLAPGWNVKYRARGRALCTIYPDTGTFTCM"
     gene            complement(257..967)
                     /locus_tag="ACZPAQ_05475"
     CDS             complement(257..967)
                     /locus_tag="ACZPAQ_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0903470.1"
                     /translation="MAKILIVDDERVLVKGIKFNLEHEGYQVQDAYDGEEAVELAREG
                     GFDLIILDVMMPKIDGLQACMRIREFSNVPIIMLTAKGEDTDKIIGFECGADDYLTKP
                     FNILELKARVRALLRRAGAAQQQRSGNELAAGHIILNTGERTASRDGVSVELTAKEFD
                     LMELLMRNPGRVYSRENLLNVVWGYEYIGDFRTVDVHIRRLREKLELDPANPEYIRTK
                     WGVGYYLSARGPRRLENV"
     gene            complement(981..1382)
                     /locus_tag="ACZPAQ_05480"
     CDS             complement(981..1382)
                     /locus_tag="ACZPAQ_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR0903471.1"
                     /translation="MTRFDKIVLGLTAAFLVVTGGYFVSGQLGEAEYQVTVSKQVPAV
                     SASLPEVEDGYPDSLLEGEVIDLNTASAADLTRLPGIGEKRAQAIVDYRESSGPFQSV
                     EELTEVKGIGEGILAQVYDYVTVGGPDSPAE"
     gene            1522..2889
                     /locus_tag="ACZPAQ_05485"
     CDS             1522..2889
                     /locus_tag="ACZPAQ_05485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0903472.1"
                     /translation="MKKYRLSSLLVTFVLALTLLAPFSALAADAPHITSRHAVLMDAN
                     YGEILYEKAAREKAYPASITKVMTALLVSEAIDRGELSQDQMITVSSTSQFDLSADGS
                     TANLKPGEVISVKDLMYCLLLPSANEAANILAEAVCGDVASFIELMNQRARELGCTGT
                     HFANTHGLHDDDHYTTAYDICLFTREALKHDLIREVVGTSVYTVPATNLSEPREFYNT
                     NALLSNWHYMGYVYDKAIGVKTGTTPEAGRCLVSAAVDGDEYLIAVILGAEPAVREDG
                     STDLKQFSESTALLKWGFRNFQRTTISQEDTPVAAVNVTLSQDANQVLVKPVGTLERT
                     LPVDMDLEAIEPTISLFQDTVEAPVKEGQVMGTMTLTYDGEVFGTLDLVATTSVDRSE
                     LLYRQEQIRQFFANSGTKLILAAVLVVAVLVLLRLLVFRKRRRYRAGAGAGGRRGSYH
                     GRRRR"
     gene            complement(2960..3118)
                     /locus_tag="ACZPAQ_05490"
     CDS             complement(2960..3118)
                     /locus_tag="ACZPAQ_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024051.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HIR0903473.1"
                     /translation="MTGGCSGNLQGISSLLKGMSVQEAIQRMEGIRCGMKSTSCPDQM
                     AQALKQFL"
BASE COUNT          557 a         1079 c          871 g          624 t
ORIGIN      
        1 catacaggta aaggtgccgg tatccggata gatggtacac agcgctcgtc ctctggctcg
       61 atattttaca ttccagccgg gggccaggct gcacccgctg aactccacag tgggggagac
      121 cccataggac tcccgaagaa agctgtgcag ctccccccaa aagggcgatc ccactccatg
      181 gcccagctcc tccagggatg gctcaccctg cttgccctgt tcttctcgct ttgacatctc
      241 ttccacacgc ctttcctcaa acgttttcca ggcggcgcgg gccacgggcg ctgagatagt
      301 accccactcc ccacttggtc cggatatact ccgggttggc cgggtccagc tccagctttt
      361 cccgcaggcg gcggatatgc acatccacgg tgcggaaatc cccgatatac tcatatcccc
      421 aaaccacgtt cagcaggttc tcccggctgt acacccggcc agggttgcgc atcagcagct
      481 ccatcaggtc gaactccttg gcggtcagct ccacggagac cccgtcccgg ctggccgtcc
      541 gctcccccgt gttgaggata atgtggcctg cggccagctc attgccgctt cgctgctgct
      601 gggcagcccc ggcccggcgg agcagagccc gcacccgggc tttcagctcc aagatgttga
      661 agggctttgt gagatagtcg tccgccccgc actcgaagcc gatgatcttg tccgtgtcct
      721 cccccttggc ggtgagcatg atgataggca cgttggagaa ctcccggatg cgcatgcatg
      781 cctggaggcc gtcgatcttg ggcatcatca cgtccaggat gatcaggtcg aagcccccct
      841 cccgggccag ctccaccgct tcctctccgt cataggcgtc ctggacctgg tagccctcgt
      901 gctccaggtt gaacttgatg ccctttacca gcacccgctc gtcgtccacg atcagaatct
      961 ttgccattgc gtctttctcc ttactcagcg ggtgagtccg gtccccccac tgtcacatag
     1021 tcatatacct gggccaggat gccctcgccg atccccttca cctcagtgag ctcttccacg
     1081 ctttggaagg ggccgctgct ctcccggtag tccacgatgg cctgggcccg cttctccccg
     1141 atccccggca gccgggtcag gtcggctgcg gaggcggtat tcagatcaat gacctctccc
     1201 tccagcaggc tgtccggata gccgtcctcc acctccggca aggaggcgct gaccgcaggc
     1261 acctgttttg agacggtcac ctggtactcc gcctccccca gctgtccgga gacaaaatag
     1321 ccccccgtca ccaccagaaa ggcggcggtg agccccagaa cgatcttgtc aaatctggtc
     1381 atggcggcca agttgttccc tcccagctgt tgcggaattc gtcagatcct gctataatga
     1441 ttgtaattat aatctcacca ggagcgggag cgctctgctc ccgcgctgat ggtagtatat
     1501 cataaatatg aggattttcc tatgaaaaaa tatcgtcttt cttccctgct ggtcaccttt
     1561 gtgctggccc tcaccctgct ggcccccttc tccgccctgg cggccgacgc cccccacatc
     1621 acctcccgcc atgccgtttt gatggacgcc aattacggtg agatcctcta cgagaaggct
     1681 gcccgggaga aggcctaccc ggccagcatc acaaaggtga tgaccgccct gctggtgtcg
     1741 gaggccatcg accggggcga gctgtcccag gatcagatga tcacggtgag cagcacctcc
     1801 cagttcgacc tctccgccga cggctccacc gccaacctga agccggggga ggtcatctcg
     1861 gtgaaggacc tgatgtactg tcttctcctc ccctccgcca acgaggcggc caacatcctg
     1921 gccgaggccg tgtgcggcga cgtagcctcc tttatcgagc tgatgaacca gcgggccagg
     1981 gagctgggct gcaccggcac ccatttcgcc aatacccatg gcctgcacga cgacgaccat
     2041 tacaccaccg cctacgacat ctgcctgttc acccgcgagg ctctgaagca cgacctgatc
     2101 cgggaggtgg tgggcacctc ggtctacacc gtccccgcca ccaacctgtc tgagccccgg
     2161 gagttctaca acaccaacgc cctcctctcc aactggcact acatgggcta tgtctatgac
     2221 aaggccatcg gcgtcaagac tggtaccacg ccggaggcgg gacgctgcct ggtctctgcc
     2281 gccgtggacg gggatgagta tctgatcgct gtcatcctgg gagcggagcc ggcggtccgg
     2341 gaggatggct ccaccgacct gaagcagttc tcggagagca ccgccctgct gaaatggggc
     2401 ttccggaatt tccagcgcac caccatctcc caggaggaca cccccgtggc cgcagtgaac
     2461 gtcaccctgt cccaagacgc caaccaagtc ctggtcaagc cggtgggcac cctggagcgc
     2521 accctcccgg tggatatgga cctggaggcc attgagccca ccatttccct gttccaagac
     2581 acggtagagg ccccggtgaa ggaggggcag gtgatgggga ccatgaccct cacctacgac
     2641 ggggaggtat tcggcaccct ggacctggtg gccaccacct ctgtggatcg ctccgagctg
     2701 ctctatcggc aggagcagat ccgtcagttt tttgccaact ccggtacgaa gctgatcctg
     2761 gccgcagtcc tggtcgtggc cgtcctcgtc ctgctccgcc tgctggtctt ccgtaagcgc
     2821 cgccgttacc gagcgggcgc cggagcaggc gggcgccggg gcagctatca tggccggcgg
     2881 cgccgctgac ctgccgaccg attcaaaaaa agaggtccgc gggcccgtct cacagacggg
     2941 atccgcggac ctttctcttt tacaggaact gtttgagcgc ctgggccatc tggtcgggac
     3001 aggatgtgga tttcatcccg cagcggatcc cctccatgcg ctggatcgcc tcctgcaccg
     3061 acatcccttt cagcagactg gagatgccct ggagattccc gctgcatccc cccgtcacct
     3121 gtacagactg g
//
LOCUS       DBMDSJ010000190         3128 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7702_length_3128_cov_1.610505, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000190 DBMDSJ010000000
VERSION     DBMDSJ010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7702_length_3128_cov_1.610505"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="ACZPAQ_05495"
     CDS             complement(<1..356)
                     /locus_tag="ACZPAQ_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR0903474.1"
                     /translation="MANVQPRTLSGFMELLPARQVQFDRMVSILRETYSLYGFTPLDT
                     PLIEASEVLLAKGGGETEKQIYRFLKGDTDLSLRFDLTVPLAKYVALNYGQLTFPFRR
                     FQIGKVYRGERAQRGR"
     gene            complement(414..635)
                     /locus_tag="ACZPAQ_05500"
     CDS             complement(414..635)
                     /locus_tag="ACZPAQ_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903475.1"
                     /translation="MANFAERIRALRKEKGLSQEEVGSVIGLKKYAVYSYEKGRACPD
                     MRGLIALADYFNVSMDYLAGRTDNPDINR"
     gene            723..1076
                     /locus_tag="ACZPAQ_05505"
     CDS             723..1076
                     /locus_tag="ACZPAQ_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903476.1"
                     /translation="MEQIYEKLYEAFVPPWATPEETAAGEAGEPYFDRLCQRFPPQEA
                     EDIWDAALSAGAAGREECFLRGFRLGALLMLDLLSGGEGERGTKTRSRPLRLGRERFK
                     HFVVPPKFRRADTPL"
     gene            complement(1281..1697)
                     /locus_tag="ACZPAQ_05510"
     CDS             complement(1281..1697)
                     /locus_tag="ACZPAQ_05510"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR0903477.1"
                     /translation="MNIALMSHDHKKELMVQFCTAYCGVLSKHTVCATNTTGRMVAEA
                     TGLPVNLFLSHAHGGIEQIGQRIIYNEIDMVLFFNDPRSGELDDSVLSISKLCDQHSV
                     PMATNVATAELLIHGLARGDLDWREVMNPNRIPFAI"
     gene            complement(1738..2472)
                     /gene="minD"
                     /locus_tag="ACZPAQ_05515"
     CDS             complement(1738..2472)
                     /gene="minD"
                     /locus_tag="ACZPAQ_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR0903478.1"
                     /translation="MGTAIVVTSGKGGTGKTSITGGVAGSLARLGHSVLCIDMDIGLR
                     NLDISLGLSDRALMDFSDVAEGRCPLVRAAVNHPDLPGLALLTAPMTLSPALTEEAVR
                     RMLDTARSLYDYVLIDSPAGLGAGFRMACCGADRAIVVSTNDASSLRDAQRTVGELDF
                     LRQIHLVMNRIQPKLLRQLRTTIDDAMDTAGLPLLGVVPEDPQVMLSANLGRPLILGG
                     RQGAANACLNIAKRLEGRRVPIMRIR"
     gene            complement(2642..>3128)
                     /gene="rsmG"
                     /locus_tag="ACZPAQ_05520"
     CDS             complement(2642..>3128)
                     /gene="rsmG"
                     /locus_tag="ACZPAQ_05520"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR0903479.1"
                     /translation="GTGAGFPGLPLKILVPSLQVTLLDSLNKRVDWLAQVSAQLSLTG
                     IRVIHARAEEQALEKGFRDSFDLVTARAVAELRLLCELCLPYVKVGGAFLAMKSVDCG
                     RELEEAAHCIKLLGARLEECRDYTIPGTDVIHRLVVLRKVAPTLKGYPRRWAKIQKAP
                     L"
BASE COUNT          574 a          924 c         1058 g          572 t
ORIGIN      
        1 aagcggcccc gctgggcccg ctctccccgg tagaccttgc cgatctggaa gcgacggaag
       61 gggaaggtga gctggccgta gttcagcgcc acatacttgg ccagggggac ggtgaggtca
      121 aagcggaggg acaggtcggt gtctcccttg agaaagcggt agatctgctt ctccgtctcg
      181 ccgccgccct tggccagcag gacctcgctg gcctcgatga ggggggtgtc caggggggtg
      241 aagccgtaga gggagtaggt ctcccgcagg atggagacca tccggtcgaa ctggacctgc
      301 cgggcgggga gcagctccat aaagccggac agggtgcggg gttgtacgtt tgccatatcg
      361 attctccttt ttggaatggt tgggggagtc aaacaacaga tcagggtgca gggtcagcgg
      421 ttgatgtcgg ggttgtcggt gcggccggcc aggtagtcca tggagacgtt gaagtagtcc
      481 gccaaggcga tgaggccccg catatcggga caggcccggc ccttttcata ggagtagacc
      541 gcatattttt tcagcccgat gacgctgccg acctcttcct gagagagtcc tttttctttg
      601 cggagggctc gaatccgctc tgcgaaattg gccatagttc ctcccaattt ttacttgaca
      661 gtagcaaatg aaaccactat actaaaagta gtttaaaatg ctacttttag ggggttgaca
      721 taatggagca gatatatgaa aaactgtatg aagcctttgt cccgccctgg gccacgccgg
      781 aggagaccgc agccggtgag gccggtgagc cctattttga ccggctctgc cagcgtttcc
      841 cgccccagga ggcggaggac atctgggacg cggcgctgtc agcgggggcc gctggccggg
      901 aggaatgctt cctccggggc ttccgcctgg gggccctgct gatgctggac ctcctctccg
      961 gcggggaagg ggagcggggg acaaaaacgc gctcccgtcc cctgcgattg ggacgagagc
     1021 gtttcaaaca cttcgtggtg ccacccaaat tcaggcgtgc ggacacgccc ctttgaccct
     1081 gtgttgcggc agggggaccg cgcccgtctc cgggcccgct ggtggcgctg tcttctccgg
     1141 ggccgtctcc gtaaggtcct ttcagccgag ggacctctct ctgtccggcg gtgtcccgga
     1201 tactcatgcg ccgtcttggc ggtggacgct gtccgcagcc cgcgcggggg cgggccgggg
     1261 gaatggggag aacgtctccc tcagatggcg aaggggatgc ggttggggtt catcacctcg
     1321 cgccagtcca agtctccccg ggccagaccg tggatcagca gctcagcggt ggccacgttg
     1381 gtggccatgg ggacggagtg ctggtcacac agcttggaga tggacagcac gctgtcgtcc
     1441 agttctccgg agcgggggtc gttgaagaac agcaccatgt cgatctcgtt gtagatgatg
     1501 cgctgaccga tctgctcgat gccgccgtgg gcgtgggaca ggaacagatt gacaggaaga
     1561 ccggtggcct ccgcgaccat gcggccggtg gtgttggtgg cgcatacggt gtgcttggac
     1621 aggacgccgc agtaagcagt gcagaattgg accatcagtt ccttcttatg gtcgtggctc
     1681 atcagtgcga tgttcatggt gtggcaaacc tcctttatac agatgccgga agggagttca
     1741 gcggatccgc atgataggga cccggcgccc ctccagacgc ttggcaatgt tcaggcaggc
     1801 gttggccgcc ccctgccgtc cgcccaggat caggggccgc cccaggttgg cggacagcat
     1861 cacctggggg tcctccggca ctacgcccag cagtggcagg ccggcggtgt ccatggcgtc
     1921 gtcaatggtg gtgcgcagct gacgcagcag cttgggctgg atgcggttca tcaccagatg
     1981 aatctgccgc agaaagtcca gctcacccac ggtgcgctgg gcgtcccgaa gggaagaagc
     2041 gtcgttggtg gagacgacga tggcccggtc ggccccgcag caggccatcc ggaagccggc
     2101 gcccaggccc gcgggggagt cgatgaggac atagtcatac agggatcggg cggtatccag
     2161 catgcggcgg acagcctcct ccgtcagggc aggggagagg gtcatgggcg cggtgagcag
     2221 ggccagaccc ggcagatcgg ggtggttcac cgccgcgcgg accagagggc agcgtccctc
     2281 cgccacatcg gagaagtcca tcagggcccg gtcgctgagg cccagggaga tgtccaggtt
     2341 gcgcaggccg atgtccatgt cgatgcacag cacgctgtgc cccaggcggg ccagactgcc
     2401 ggccacaccc ccggtaatgg aggtttttcc ggtgccgccc ttcccggagg tgacgacgat
     2461 tgcggttccc atgtgtgcgc agacctccca tcacaccatt ttaagtgtat cacaaatccc
     2521 acccacgtca atgggtttat gagaaatttt gaaaaatgga gccgaaaatc caggtaaagc
     2581 cctaccctct gtacactgag ggtatcgatc ctggagggct ggcggagaca tcgctccggt
     2641 ctcacagcgg ggctttctgg atcttggccc accgccgggg atagcccttc agggttgggg
     2701 ccaccttccg caggactacc agacggtgaa tgacgtcggt gccgggaatg gtgtagtccc
     2761 ggcactcctc cagacgggca cccagcagct tgatgcagtg ggcggcctcc tccagctccc
     2821 ggccgcagtc caccgacttc atggccagaa aagcgccccc taccttgaca tagggcaggc
     2881 acagctcaca cagcaggcgg agctcggcca ccgcccgggc agtgaccaga tcaaagctgt
     2941 cccggaagcc cttctccaga gcctgctcct ccgcacgggc atggatgacc cggatgccgg
     3001 tgagggagag ctgggcgctg acctgagcca gccagtccac ccgcttgttc aaactgtcca
     3061 gcagagtgac ctggagggag gggaccagga tcttcagggg cagacccgga aagccggccc
     3121 cggtgccc
//
LOCUS       DBMDSJ010000191         3120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7722_length_3120_cov_1.340841, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000191 DBMDSJ010000000
VERSION     DBMDSJ010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7722_length_3120_cov_1.340841"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(136..504)
                     /locus_tag="ACZPAQ_05525"
     CDS             complement(136..504)
                     /locus_tag="ACZPAQ_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR0903480.1"
                     /translation="MSGGESRLLGRWGEALAAEELRRRGCRLVEAGWRCRFGEVDLIA
                     EEGPFLCFVEVKLRKDGRVAQAREFVDRRKQEKLRTTAQLYLAEHPTQLQPRFDVVEI
                     YAPQGLATRAPEIYVIPDAF"
     gene            complement(501..1106)
                     /locus_tag="ACZPAQ_05530"
     CDS             complement(501..1106)
                     /locus_tag="ACZPAQ_05530"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR0903481.1"
                     /translation="MGPGEYEQKAVEAGFVRVCGCDEAGAGPLAGPVYAAAVILAQGA
                     DIPGLNDSKKLTEKKREALFPLIQQSALAWSVARVEAEEIDRTDILSARIRAMQLAIE
                     GLSLRPDFALIDGNRDHGRSAAITVPHWCVVKGDSISPSIAAASILAKVSRDRFMLEM
                     AERYPQYGFERHKGYGTRLHYEMLRRYGPSPIHRRTFLKDL"
     gene            complement(1133..2017)
                     /gene="ylqF"
                     /locus_tag="ACZPAQ_05535"
     CDS             complement(1133..2017)
                     /gene="ylqF"
                     /locus_tag="ACZPAQ_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HIR0903482.1"
                     /translation="MNIQWYPGHMTKTRRQIEADLKHVDIVAEIIDARIPISSRNPDI
                     DAICADKPRLIVLNRADQADPAQNRAWGEYFRKKGYAVLETDAKTGRGVNQFSSVTQE
                     VLKEQISKWREKGQVGRPVRAMVVGVPNVGKSTFINKVARKKSAKAGDRPGVTRGKQW
                     VNVDSALDLLDTPGILWPKFEDPHTGMVLAFTGAVKDEIMDVETLGCHLMAYLGEHYP
                     QVLTQSYKLPEVPARQEEENDIAYGYRLLEAGAAKRGMRISGGELDTERMARVLLDEY
                     RAGKLGRFTLETPEGTSI"
     gene            complement(2121..2462)
                     /gene="rplS"
                     /locus_tag="ACZPAQ_05540"
     CDS             complement(2121..2462)
                     /gene="rplS"
                     /locus_tag="ACZPAQ_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR0903483.1"
                     /translation="MDLMQAFTQKHLKAEPPVVEVGDTVRVHIKVKEGNRERIQVFEG
                     TVIAKKHGGLEETFTVRRVSYGVGVEKVFPVHAPSIEKIETVRHGKVRRAKLYYLRDR
                     VGKSAKIKEAL"
     gene            complement(2613..>3120)
                     /locus_tag="ACZPAQ_05545"
     CDS             complement(2613..>3120)
                     /locus_tag="ACZPAQ_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR0903484.1"
                     /translation="CAHTNLDAAQGGVNDCLARALELDGVELLCRDGTDGQGRPYGIG
                     RVGTAPQAGCSAAEFAAFIKEKLSACSVRFVETGRPVRRVAVGGGACASMLSDAMAAG
                     CDTFVTADVKYDQYLEAKALGINLMDAGHFATEDVVCPALVQFLSRHFPKVEVRRTET
                     HREAYDSV"
BASE COUNT          525 a         1070 c          883 g          642 t
ORIGIN      
        1 caatccaatg ctttattatg ccagttcttt tctctccggt caagcgtttg tcgcaaaaaa
       61 gaggggcagt ctccccttct ccccgatttt ctccccccgg gccccgcccg ttttccagat
      121 tggggcagag ggacgtcaaa aagcgtcagg gatgacatag atctccgggg cccgggtcgc
      181 cagcccctgg ggcgcgtata tctccactac gtcgaacctg ggctggagct gcgtgggatg
      241 ctccgccaga tagagctggg ccgtggtccg cagcttctcc tgcttcctcc ggtccacgaa
      301 ctcccgcgcc tgggccaccc gtccgtcctt ccgcagcttg acctccacaa agcagaggaa
      361 aggcccctcc tccgcgatca ggtccacctc gccaaaccgg cagcgccagc cggcctccac
      421 caggcggcac ccgcgccggc gcagctcctc cgccgccaga gcctctcccc accggcccag
      481 cagcctgctc tctcccccgc tcacaggtct tttaaaaagg tgcggcggtg gatgggactg
      541 gggccgtacc ggcgcagcat ctcatagtgc agtcgggtcc cgtacccctt gtgccgctcg
      601 aacccgtatt gtggatagcg ctccgccatc tccagcatga agcggtcccg gctcaccttg
      661 gccagaatgg aggcggcggc gatggagggc gagatgctgt cccctttcac cacacaccag
      721 tggggaacgg taatggcggc agagcgcccg tggtcccggt ttccgtcgat cagggcaaaa
      781 tccgggcgga gggagagccc ttctatggcc agctgcatgg ccctgatccg cgcgctgagg
      841 atatcggtcc ggtcgatctc ctcagcctcc acccgggcta ccgaccaggc cagcgcactc
      901 tgttggatga gtggaaagag cgcctcccgt tttttttctg tcagcttttt ggagtcattc
      961 aggccgggga tgtccgcgcc ctgggccagg atcaccgccg cggcgtacac cggtccggcc
     1021 agaggtccgg cccccgcctc gtcacagccg cagacccgga caaagccggc ctccactgct
     1081 ttctgctcat actctcccgg tcccatgcct tccttccttt tctccaggct gctcatatag
     1141 acgttccttc tggtgtctct agtgtaaagc gtcccagctt cccggccctg tattcatcca
     1201 gcaggacccg agccatccgc tccgtgtcca actcgccgcc ggagatgcgc atcccccgct
     1261 ttgccgcgcc ggcctccagc agccggtagc cgtaggcgat gtcgttttcc tcctcctggc
     1321 gggcaggaac ttccggcagc ttgtaactct gggtcaggac ctggggatag tgctcaccaa
     1381 gataggccat tagatgacag cccagggtct ccacatccat gatttcgtct ttgacagcgc
     1441 ctgtaaaggc aagtaccatg ccagtgtgcg ggtcctcaaa tttcggccac aggatgcccg
     1501 gtgtgtccag cagatccagg gcactgtcca cgttgaccca ctgcttgccc cgggtaacgc
     1561 cggggcggtc ccccgcctta gccgacttct tccgggccac cttattgata aaggtggact
     1621 ttcccacgtt ggggactccc accaccatgg cccgcaccgg acggcctacc tggcccttct
     1681 cccgccattt tgagatctgc tccttcagca cttcctgggt cacagaggag aactggttta
     1741 caccccggcc ggtcttggca tcagtctcca gcaccgcata tccttttttc cggaaatact
     1801 cgccccaggc ccggttctgg gccgggtcgg cctgatctgc ccggttcagc acgatcagcc
     1861 ggggcttatc ggcgcagatg gcatcgatgt ccgggttccg gctggagatg gggatgcggg
     1921 cgtcgatgat ctccgccacg atgtccacat gcttcaggtc cgcctcgatc tgccgtcggg
     1981 tcttggtcat atgtcccgga taccactgaa tattcaactc tcgtcttctc cttccctacg
     2041 gcggagggcg tgctccgctc tccccgccgc catggtataa aaagggagcg gtaccagtac
     2101 cgctcccttt ttatgttgga ttacagggct tccttgatct tggcggactt gcccacgcgg
     2161 tcgcgcaggt agtacagctt ggcgcggcgg accttaccgt ggcggacggt ctcgatcttc
     2221 tcaatggagg gagcgtggac ggggaacacc ttctccacac cgacgccgta ggacacgcgg
     2281 cggacggtga aggtctcctc cagaccgccg tgcttcttgg cgatgacggt gccctcaaag
     2341 acctggatac gctcgcggtt gccttccttg accttgatgt gcacccgcac ggtgtcgccc
     2401 acctctacga cggggggctc agccttcagg tgcttctgag taaaagcctg catgagatcc
     2461 ataatgattc ctcctgttta tataggcgtt cttgccggcc gtctgccccg ctgtcgctgg
     2521 ggcgccgcag aggaccgccc gttatcagac caaaggatta taccacagca tttttcaaaa
     2581 tgcaagtaga atttccctgt ttttcgcctt tctcacaccg aatcataagc ctcccggtgg
     2641 gtctccgtcc gccggacctc caccttcggg aagtgccggg agaggaactg caccagcgct
     2701 gggcacacta catcctctgt ggcaaaatgg cccgcgtcca tcagattgat gcccagggcc
     2761 tttgcttcca ggtattggtc gtacttcacg tcggcagtga cgaaagtgtc acaccccgcc
     2821 gccatggcgt cggagagcat ggaggcgcag gcgccgcccc ccaccgccac ccggcgcacc
     2881 ggccggcccg tctccacgaa acggacgctg caggcggaga gcttttcctt gataaaggcg
     2941 gcaaattcag cggcggaaca gccggcctga ggggccgtcc caacccggcc gatgccgtag
     3001 ggccgcccct gtccgtcagt cccgtcccgg cagagcagtt ccacaccgtc cagctccagg
     3061 gcccgggcca gacagtcatt gacgcccccc tgggcagcgt ccagattggt gtgggcacac
//
LOCUS       DBMDSJ010000192         3106 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7761_length_3106_cov_1.440266, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000192 DBMDSJ010000000
VERSION     DBMDSJ010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7761_length_3106_cov_1.440266"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(91..849)
                     /gene="yaaA"
                     /locus_tag="ACZPAQ_05550"
     CDS             complement(91..849)
                     /gene="yaaA"
                     /locus_tag="ACZPAQ_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR0903485.1"
                     /translation="MKILISPAKKMRTDTDTLSPQALPAFLPETERLLSALRSLSRQE
                     LKQLWRCSDAITDLNVERLARMELGKGLTPALLSYQGIQYQYMAPGVFETGQFTYLQE
                     HLRILSGFYGMLRPFDGVTPYRLEMQARLSVNGCPDLYAFWGDRLARALAGEAGLVVD
                     LASQEYSRAVLPHLPPSVEVLTCTFGELRKDGRVVEKGTLCKMARGQMVRWMAERGVR
                     RSEELKDFQDLGYRYDAAHSGHDHYVFLKEKEEF"
     gene            complement(846..1547)
                     /locus_tag="ACZPAQ_05555"
     CDS             complement(846..1547)
                     /locus_tag="ACZPAQ_05555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0903486.1"
                     /translation="MDTMTLTLNHASVPAVLYGSPSPQVWLCLHGKGGHKEEAESFAQ
                     VVCPKGWQVLAIDLPGHGARSGGPELFTPWHAVPELRDLLSLAGQKWNRLALRAVSLG
                     AWFSLLAFQDRPLDRALFVSPVLDMERLIQEMMSWAGVTESRLEAEGEISTEFGETLS
                     WPYLQYVRAHPVSRWETPTRILWAQKDHLVPRDTVDRFARRFRCSLTAAEGMEHWFHT
                     PEQLAVLRQWEDEST"
     gene            complement(1602..2636)
                     /locus_tag="ACZPAQ_05560"
     CDS             complement(1602..2636)
                     /locus_tag="ACZPAQ_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR0903487.1"
                     /translation="MLAYTYLERGKFALLDKPKPVLLDPGDAVVRVTLASICTSDLHI
                     KHGSVPRAVPSVTVGHEMVGVVEETGPDVRTLRPGDRVAVNVETFCGSCFFCRHGWVN
                     NCTDPSGGWALGCRIDGGQAEYVRVPYADQGLTKIPHAVTDRQALFVGDILATGYWAA
                     QISQISPEDTVLVLGAGPTGLCTLQCVLLENPRRVIVCERDPQRLAFVRAHYPQVLAL
                     EPEGLREAVLAHSAHGGADVVLEVAGSEDTFRLAWECARPNAVVTVVALYDRPQLLPL
                     PDMYGKNLTFKTGGVDGCNCAQTLDLIAAGKLDTTPLITHTYPLKDIDAAYQLFESRR
                     EGVIKVAIQP"
     gene            complement(2869..>3106)
                     /locus_tag="ACZPAQ_05565"
     CDS             complement(2869..>3106)
                     /locus_tag="ACZPAQ_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903488.1"
                     /translation="ENKAIDALKFKEELCGPHGVLLLGKLLDPNGNELEATDSTIIKL
                     AQEQSEASLSYQDEIPWGVCRLLQLFQGTNYQDI"
BASE COUNT          582 a          954 c         1029 g          541 t
ORIGIN      
        1 attgcttcca tcagcgcagg taatatgaga ccgggtttgg caagagcgcc aaacccggtc
       61 gtttttcagc catcccagct tccagtatgt tcaaaattcc tctttttcct tgagaaagac
      121 atagtgatcg tgcccggaat gggcggcgtc ataccggtaa cccagatcct gaaagtcttt
      181 cagctcctcg ctccggcgga cgccacgctc tgccatccat cggaccatct gcccccgggc
      241 catcttgcac agcgtgccct tttccaccac ccggccgtcc ttacgcagct cgccgaaggt
      301 gcaggtgagc acctccacag aggggggcag gtgggggagc accgcccggc tgtactcctg
      361 ggaggccagg tccaccacca agccggcctc tccggccagc gctcgggcca gcctgtctcc
      421 ccagaaggcg tacagatcag ggcagccgtt caccgacaga cgggcctgca tctccaggcg
      481 gtagggcgtc acgccgtcga aggggcggag catgccataa aagccggaga ggatgcgcag
      541 atgttcctga agataggtga actggccggt ctcaaagaca ccgggggcca tgtactgata
      601 ctggatgccc tgataggaga gcagggcggg ggtgaggccc ttccccagct ccatgcgggc
      661 cagccgctcc acattcagat cagtgatggc atcgctgcac cgccacagct gcttcagctc
      721 ctgccgggac agggaacgca gagcggagag caggcgctcc gtctctggga gaaaggcggg
      781 cagagcctgg ggagagagcg tgtcggtgtc ggtccgcatc tttttggcgg gggagatgag
      841 gatcttcatg tgctctcatc ctcccactgc ctcagaaccg ccagctgttc cggggtatgg
      901 aaccagtgct ccatcccctc cgccgcagtc agggagcagc ggaagcggcg ggcaaagcgg
      961 tccactgtgt cccggggcac aagatggtcc ttttgggccc agaggatgcg ggtgggggtc
     1021 tcccagcggg agacagggtg ggcgcggacg tactggagat agggccagga cagggtctca
     1081 ccgaactcag tggaaatctc tccctcggcc tccaggcgtg actcggtcac cccggcccag
     1141 gacatcatct cctggatcag gcgctccatg tccagcaccg gggagacgaa gagggctcgg
     1201 tccagggggc ggtcctgaaa ggccagcagg gagaaccagg cccccaggct gacggcccgg
     1261 agggccaggc ggttccactt ctgtccggcc agggagagca ggtctctcaa ctcggggaca
     1321 gcgtgccagg gggtgaagag ctccggcccg ccggaccgtg ccccgtggcc gggcaggtcg
     1381 atggccagta cctgccagcc cttggggcag accacctggg cgaaggactc ggcctcctcc
     1441 ttgtggccgc ccttgccgtg gaggcagagc cagacctgag gggacgggga gccgtagagg
     1501 acagcgggga cggaggcgtg gttcagagtc agagtcatgg tatccataag gttcctcccg
     1561 ctccctaggg agcgatctgt cgtaggatgg ctggggaggc gtcagggctg gatggccacc
     1621 ttgatgacgc cctcccgccg ggactcgaag agctggtagg ccgcgtcgat gtccttgaga
     1681 gggtaggtgt gggtgatgag gggagtggtg tccagcttcc cggcggcgat caggtccagg
     1741 gtctgggcac agttgcagcc atccaccccg ccggtcttga aggtgagatt cttgccgtac
     1801 atgtcgggca gagggagcag ctgcggccgg tcgtaaaggg ccaccaccgt caccaccgca
     1861 ttgggcctgg cgcactccca ggccagacgg aaggtgtcct cgctgcccgc cacctccagc
     1921 accacatccg cccctccgtg ggcgctgtgg gccaggaccg cctcccggag cccctccggc
     1981 tccagtgcca gcacctgagg atagtgggca cggacgaaag ccaggcgctg ggggtcccgc
     2041 tcacagacga tgacccgcct ggggttttcc agcaggacgc actggagagt gcacaggccg
     2101 gtggggccgg cgcccaggac gagcaccgtg tcctccggtg agatctggga gatctgagcg
     2161 gcccagtatc cggtggccag gatgtccccc acgaacaggg cctgccggtc cgtcacggcg
     2221 tgagggatct tggtcagccc ctggtcggca taggggaccc ggacatactc cgcctgcccg
     2281 ccgtcgatgc ggcagccaag ggcccagccg ccgctgggat cggtgcagtt gttgacccag
     2341 ccgtgtcggc agaaaaagca ggagccgcag aaggtctcca cattgacggc cacccggtct
     2401 ccggggcgga gggtacggac gtccgggcct gtctcctcca ccacgcctac catctcgtgt
     2461 cccaccgtga cgctgggtac cgcccgggga acagagccgt gcttgatgtg cagatcgctg
     2521 gtgcagatgc tggccagcgt gacccggacg accgcgtccc cggggtccag caggaccggc
     2581 tttggcttgt ccagcagggc gaatttcccc cgctccagat aggtgtaggc gagcatggat
     2641 cagacctcct gtgttttgag atggatgaag gcggcgccgg atcggagaga aggtgccggt
     2701 gcccatgtca cacagcgcgc cggaacccag ggcgcgccct aacagcgccg cccggcctgc
     2761 ccattatacc tcaggaagag aaaaacagca actcgggaag ttagggggag cggcagggga
     2821 catgtgatga taaaacctga gaggaactaa ggccgtcagg cagtttggtc aaatgtcctg
     2881 atagtttgtt ccctgaaaaa gctgcagtaa tctgcagact ccccaaggaa tttcatcctg
     2941 ataggacaga gaggcttctg actgctcctg cgctagtttt atgatggtgc tgtctgttgc
     3001 ctcgagttca tttccgtttg gatctaaaag ctttcccaat aacagcactc cgtgcggccc
     3061 gcagagttcc tctttaaatt tcaaggcgtc aatagcctta ttttct
//
LOCUS       DBMDSJ010000193         3095 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7792_length_3095_cov_1.686039, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000193 DBMDSJ010000000
VERSION     DBMDSJ010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7792_length_3095_cov_1.686039"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="ACZPAQ_05570"
     CDS             complement(<1..255)
                     /locus_tag="ACZPAQ_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR0903489.1"
                     /translation="MDILVSACLLGTPCRYDGASRPCGELARLEVLGHRLIPVCPEVM
                     GGLPTPRPPAELQRDGRVVDRAGKDVTEAYRLGAEEVLELA"
     gene            complement(324..575)
                     /locus_tag="ACZPAQ_05575"
     CDS             complement(324..575)
                     /locus_tag="ACZPAQ_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR0903490.1"
                     /translation="MDEMDFTRKFRLGDQRDLEIKAILTTVYQALQEKGYNPVNQIVG
                     YILSEDPTYITNHNNARALIRKVDRDELLQTLVKYYLTH"
     assembly_gap    649..658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(690..1844)
                     /locus_tag="ACZPAQ_05580"
     CDS             complement(690..1844)
                     /locus_tag="ACZPAQ_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903491.1"
                     /translation="MDELEFAQRLKRYRRERGLTQQELADRIGVSNKSVSRWESGSYP
                     DVPTLLSLARALDVTVDALLDPGAPVPTLQKSDWQNLLSFAFAIGGGLAFYLLRRFVP
                     VPVCWALYLGCLAYGIYLQAHYTYHARWFRLGSWAMCFCVSWTLAEELGGVVFQVLFP
                     TVLSQWKNMIFQLLRGDAGALLQLVLVLGPLWLAGACGLTVLTQLAARRLAGERGDLP
                     VRLPDLRGHPRLARAPFAWSKAMPALCPIVLAGFWYLYRQTLLPRFWYQHQQELYLAV
                     WTMAGVLALLPCLKRRHWGMLLPVLAMMLACLGFRGMLAYPQVYSLFSGRLMEEKAEM
                     STQYYLRFGQPSEELAVAALLVAAVYLLCCFLRVEWVSAEQKGRQEPEES"
     gene            complement(2335..>3095)
                     /locus_tag="ACZPAQ_05585"
     CDS             complement(2335..>3095)
                     /locus_tag="ACZPAQ_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR0903492.1"
                     /translation="LNPLGEDTSIRRTTVLPSILEILTRHYNFRNKCAKLYEVGRIYL
                     PGGADGLAVEKKVLSLGAYGEGMDFFALKGAVEAILGEIRAADVTFEAPQIPNPSYHP
                     GRCAQVLVNGQEVGVFGQIHPLVAQNYGVDAEFYCAELDFDQLMCGQGPDPQYVPLPK
                     FPAVTRDIAVVCGEAVTVGALEDCIRRGAKGLLKEVTLFDIYRGKGVAEGKKSVAFNL
                     VLRADDRSLTAEEADADVKAILETLEKELGAVLR"
BASE COUNT          636 a         1016 c          888 g          545 t
ORIGIN      
        1 ggccagctcc agcacctctt cggctccgag gcggtacgcc tccgtcacgt cctttcccgc
       61 ccggtccacc acccggccgt ctctctggag ctccgccggc gggcggggcg tgggcaggcc
      121 gcccatcacc tccgggcaca cggggatcag ccggtgcccc agcacctcca gccgcgccag
      181 ctccccgcag ggcctggagg ccccgtcgta ccggcaggga gtccccagca ggcaggcact
      241 gaccaaaata tccacctgtc tctccccttc cccaacatca gaaaagggcc cgctgcgcct
      301 tgcagcgggc cctcgatctg ctgtcaatgg gtcaaataat atttcaccaa agtctggagc
      361 agctcatccc ggtccacttt tcggatcagc gccctggcgt tattgtggtt ggtgatatag
      421 gtgggatctt ctgagaggat atagcccacg atctgattga ctgggttata gcccttctcc
      481 tgaagcgcct gatacaccgt agtcaagatc gccttgatct ccagatcccg ctgatcgccc
      541 agccggaact tccgagtgaa atccatctca tccatcgaac acacctcctg cagcgttgaa
      601 gtggaacact tgtgcttagt ttactccaaa aaggaggcgg tgtaaaggnn nnnnnnnnaa
      661 agggtatggg gcaaaatttt aagaaaaatt taggactcct ccggttcctg ccggcccttc
      721 tgctccgccg acacccactc cacccgcagg aagcagcaca gcaggtacac agcggccacc
      781 agaagggcgg ccaccgccag ctcctcgctg ggctggccga agcgcagata atactgggtg
      841 gacatctctg ccttttcctc catcagcctg ccggaaaaca gggaatagac ctggggatag
      901 gccagcatcc cccggaaccc caggcaagcc agcatcatgg ccaggaccgg gagcagcatc
      961 ccccaatgcc tccgcttcag gcaggggagc agagccagca cccctgccat cgtccagacc
     1021 gccagataga gttcctgctg gtgctggtac cagaatctcg gcaggagagt ctgccggtag
     1081 agataccaga acccggccag gacgatgggg cacagagcgg gcatggcctt gctccaggcg
     1141 aagggggccc gggccagccg gggatgcccc cgcaggtcgg gcagccgcac cgggagatcc
     1201 cctctctccc ccgccagccg ccgggcagcc agctgagtca gaacggtgag gccgcaggcg
     1261 cccgccagcc ataggggccc cagaacaagg accagctgaa gcagcgcgcc ggcatcccct
     1321 ctcagcagtt ggaaaatcat gttcttccac tggctcagaa ctgtgggaaa aagcacctga
     1381 aacaccacgc cccccagttc ttccgccagg gtccaactga cgcaaaagca catggcccag
     1441 ctgcccaggc ggaaccagcg ggcgtgatag gtgtagtgag cctggagata gatgccgtag
     1501 gccagacagc ccagatagag ggcccagcac accggcacgg ggacgaacct ccgcagtaaa
     1561 taaaaggcca gtcctccgcc gatggcgaag gcaaaggaga gcagattctg ccagtcgctt
     1621 ttctgcaatg tggggacagg ggcacctggg tccagcaggg catccaccgt cacatccagt
     1681 gcccgggcca gagagagcag ggtagggaca tccgggtagc tcccgctctc ccaccgggag
     1741 actgatttgt tggacacgcc gatgcgatcg gccagctcct gctgggtgag cccccgctcc
     1801 cggcggtacc gtttcagccg ctgggcaaat tccagttcat ccatggtgtc tcctcctgtg
     1861 atcctttctt gcctctatca taccggaaaa acgttcccgc cgccaccccg gagggtgaga
     1921 atctgctctc gcgggacagg aatttgttcc cggaagtgga aaatcaggtc tcaggccgca
     1981 gggggcacat aggatgcgtc ccctgcaaga tgttgtaaac ctccggctca cagcactgtg
     2041 cattgccgac cggctcagct gtctgtacag gtctcgcgat ctcgtccatc actctttctt
     2101 tccgggatca caaataaaag gatcaaatgc caataggaat aatataaaaa cagacaacat
     2161 atcccataca tcaggtacag gatcgcccgc cctgctgccg tctctggctt cgtactgctt
     2221 tatggttaca tcgggcataa aaagcggggg cgaaactaag tttcgccccc gccaaaactt
     2281 ttctgcggga aaacgcttgt acgcgctgcg cgccccgtct gccacggggc ggcctcagcg
     2341 cagtaccgcg cccagctcct tttccagagt ttccaaaatc gctttcacat cggcgtccgc
     2401 ctcttcggcg gtcaggctgc ggtcgtcagc ccggaggacc aggttgaagg ccacagactt
     2461 cttgccctcg gccacgccct tgccccgata gatgtcgaac agggtgacct ccttcagcag
     2521 tcccttcgcc ccccgacgga tgcagtcctc caaggctccc acggtgaccg cttcgccgca
     2581 caccacagcg atatcccggg tgacggcagg gaacttgggc aggggcacgt actggggatc
     2641 ggggccctgc ccgcacatca gctggtcaaa gtccagctcg gcgcagtaga actctgcgtc
     2701 cacgccgtag ttctgggcca ccagggggtg gatctgacca aacacgccca cctcctggcc
     2761 gttgaccagc acctgggcac agcggcctgg gtggtaggag ggattgggga tctgaggggc
     2821 ctcaaaggtg acgtccgccg cccggatctc tcccaggatg gcctccaccg cccccttcag
     2881 ggcaaagaag tccattcctt cgccgtaagc gcccagagag agcaccttct tctccaccgc
     2941 caggccgtcg gccccgccag ggagatagat gcggcccacc tcatacagct tggcgcactt
     3001 gttgcggaag ttgtagtgcc gggtcaggat ctccagtatg gaggggagca ccgtggtgcg
     3061 cctgatggag gtatcctccc ccagagggtt gagga
//
LOCUS       DBMDSJ010000194         3085 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7824_length_3085_cov_1.985255, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000194 DBMDSJ010000000
VERSION     DBMDSJ010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7824_length_3085_cov_1.985255"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            339..1238
                     /locus_tag="ACZPAQ_05590"
     CDS             339..1238
                     /locus_tag="ACZPAQ_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903493.1"
                     /translation="MRKDIALPVLALAGGGACLALRRWQLAAALDPETELFRSVPATP
                     VLIGVLALLALLFLVLIQGGGKPEDYPAAFRCPSSLYMTLVTAGSLLLLAAAALGTLG
                     FFRAFQLWRDGLGQGLPVMELLAAVLCIPSAAALMILGRGHYRGGLPACYPGLTTLPG
                     YAALPWLVGIYQEYSRDPVVLRFADMILAVVCAVLALYFAASFAFRHPRPRLCLFFSL
                     MGIVLNLEALANGQSLFFTALTAAAVLVLLAQSWALLRTCFGPAWPECLLEDRMPSGA
                     LERAEGDNAPGGAGVENHHREDD"
     gene            1241..>3085
                     /gene="metG"
                     /locus_tag="ACZPAQ_05595"
     CDS             1241..>3085
                     /gene="metG"
                     /locus_tag="ACZPAQ_05595"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR0903494.1"
                     /translation="MERKRFYITTPIYYPSDKLHIGHTYCTVATDAMARYKRLTGCEV
                     MFLTGTDEHGQKIEDKAKEAGVTPKEYVDRIVEGERGVLDLWKLMNISNDRFIRTTDG
                     YHVSAIQKIFKKMYEKGDIYKGKYVGKYCKPCESFWTESQLVDGKCPDCGRDVVDAEE
                     EAYFFRLSKYADRVQHLLEDTDFLEPRSRVNEMVNNFIKPGLEDLCVSRTSFTWGVPV
                     DFDPGHVVYVWVDALFNYTTALGFLNDRYDDYEKFWPADVHFVGKEIVRFHSIIWPAM
                     LMSMEMPLPKKVFGHGWLLLDGGKMSKSKGNVVDPYILAEKFGVDALRFFLMRTFPFG
                     SDGNFSNELLIQTINTDLANDLGNLLSRTVSMVGKYFGGTLPEEQAADEEKDGELVEL
                     ISSLRTRYEEQMEHYAFQNALAEIFKVIGRANKYIDENTPWTLAKDMEANGARLARVM
                     YNLLEVLRVSAILLTPFIPQSAAEVLRQIGACETCSTWESAAGYGALSHTVTVSRGEN
                     LFPRIDAEKALSELEEAAAAAKKAALPDLEVEPQLTEKVDFDTFCKSDFRAVKVKACE
                     TVKKSNKLLRFTLDDGTGTDRQILSGIAMWYKPEELVGKTLVAIVNLPP"
BASE COUNT          565 a          944 c          982 g          594 t
ORIGIN      
        1 caacgacctc agccgcggct gcaacgccca ggaggtctac tccatggcca tcatcaccgc
       61 cgctctggcg gagtaaacgg ccagacgacc acagactgcg ccggcgggcc ccagtggggc
      121 ccgtcgtttt gcgtcttgtg aggggacagg gaagaaacgg caggcacatg ggagatgcgg
      181 ttttataaaa tcgtttcggg ggcctgtacc gctttttaag atgaccacct tgaaaggact
      241 gggaaaaaaa cggcggattt tttccctctg tgttgcattt tctctgcggg taaggtatga
      301 taataaaagc gcattctccc agttttagga gtgttgtcat gagaaaagat atcgctttgc
      361 ccgtgctggc cctggcgggg ggcggcgcct gcctcgccct gcgccgctgg cagctggccg
      421 ccgcgctgga cccagagacc gaactgttcc gctccgtccc ggccacgccg gtgctgatcg
      481 gtgtgctggc cctgctggcg ctgctgttcc tggtcctgat ccagggggga gggaagccgg
      541 aggactatcc cgcagccttc cgctgtccca gctccctgta tatgaccctg gtgacggcgg
      601 ggagcctgct cctgctggcc gccgcggcgc tgggcaccct gggctttttc cgggcgtttc
      661 agctgtggcg ggacggactg ggccagggac tgcccgtgat ggagctgctg gctgccgtat
      721 tgtgcatccc ctccgctgca gcgctgatga tcctggggcg gggccattac cggggaggcc
      781 tgcccgcctg ttatcccggc ctgaccaccc tgcccggcta cgccgccctg ccctggctgg
      841 tggggatcta tcaggagtac tcccgggacc cggtggtgct gcggttcgca gatatgatcc
      901 tggcggtggt gtgtgcggtg ctggcgctgt acttcgccgc ctccttcgct ttccgccacc
      961 ccaggcccag gctgtgcctg tttttttccc tgatggggat cgttctgaac ctggaggcgc
     1021 tggccaacgg acagtccctc ttctttaccg cgctgaccgc tgctgcggtg ctggtcctgc
     1081 tggcccagag ctgggcgctg ctgcgcacct gtttcggccc ggcttggccg gagtgcctgc
     1141 tggaggatcg aatgcccagc ggggcattgg aacgggcaga aggggacaat gccccgggag
     1201 gagccggggt cgagaatcat catcgggagg acgactgaat atggaacgca agagatttta
     1261 tatcaccacc cctatctact atcccagcga caagctgcac atcggccaca cctactgcac
     1321 cgtggccacc gacgctatgg cccggtataa gcgtctcacc ggctgtgagg tcatgttcct
     1381 gacgggcacc gacgagcacg gccagaagat cgaggacaag gccaaggagg ccggcgtcac
     1441 ccccaaggag tatgtggacc gcatcgtgga gggggagcgg ggcgtgctgg atctgtggaa
     1501 gctgatgaac atcagcaacg accggttcat ccgcaccacc gatggctacc acgtgtccgc
     1561 catccagaag atatttaaga agatgtatga gaagggggac atctacaagg gcaagtatgt
     1621 agggaaatac tgcaagccct gcgagtcctt ctggaccgag agccagctgg tggacggcaa
     1681 gtgccctgac tgcggccggg atgtggtgga cgcggaggag gaggcctact tcttccgcct
     1741 gtccaagtac gccgaccggg tacagcatct gctggaggac accgactttt tggagccccg
     1801 cagccgggtg aatgagatgg tgaacaactt catcaagccc ggcctggagg acctgtgcgt
     1861 cagccgcacc tccttcacct ggggcgtgcc ggtggacttt gacccgggcc acgtggtcta
     1921 tgtatgggtg gacgccctgt tcaactacac caccgccctg ggcttcctca acgaccggta
     1981 tgacgactat gagaagttct ggcccgcgga cgtccacttt gtgggcaagg agatcgtccg
     2041 gttccactcc atcatctggc ccgccatgct gatgagcatg gagatgcccc tgcccaagaa
     2101 ggtgttcggc cacggctggc tgctgctgga cggaggaaag atgtccaagt ccaagggcaa
     2161 cgtggtagat ccctatatcc tggcggagaa gttcggagtg gatgccctgc gcttcttcct
     2221 gatgcgcacc ttccccttcg gctctgacgg caacttctcc aacgagctgc tgatccagac
     2281 catcaacacg gacctggcca acgacctggg caatctcctc tcacgcaccg tgtccatggt
     2341 gggcaagtat ttcggcggca ccctgcctga ggagcaggcg gcggacgagg agaaggatgg
     2401 ggagctggtg gagctgatct cctccctgcg caccaggtat gaagagcaga tggagcacta
     2461 cgccttccag aacgccctgg cggagatctt caaggtgatc ggccgggcca acaagtatat
     2521 cgacgagaac accccctgga ccctggccaa ggacatggag gccaacggcg cccggctggc
     2581 ccgggtgatg tacaacctgc tggaggtgct gcgggtctcc gctatcctgc tcaccccctt
     2641 tatcccccag tctgctgccg aggtgctccg gcagatcggc gcctgcgaga cctgctctac
     2701 ctgggagagc gccgccggct atggggccct gtctcacact gtcactgtct cccggggcga
     2761 gaacctgttc ccccgcatcg acgcagaaaa ggccctttcc gagctggagg aggctgcggc
     2821 ggccgccaag aaggcagctc tgcccgattt ggaggtggag cctcagctca cagagaaggt
     2881 ggattttgac accttttgca agagcgattt ccgggccgtt aaagtcaagg cctgcgaaac
     2941 agtgaagaag tccaacaagc tgctgcgctt caccctggac gacggcaccg gcaccgaccg
     3001 gcagatcctc tccggcatcg cgatgtggta caagcccgag gaactggtgg gcaagaccct
     3061 ggtcgccatc gtcaacctgc ccccc
//
LOCUS       DBMDSJ010000195         3053 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7921_length_3053_cov_1.564024, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000195 DBMDSJ010000000
VERSION     DBMDSJ010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7921_length_3053_cov_1.564024"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            280..474
                     /locus_tag="ACZPAQ_05600"
     CDS             280..474
                     /locus_tag="ACZPAQ_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HIR0903495.1"
                     /translation="MYRYEYVTYRTGGGFWMDNSACEHREIIDEYAAKGWRYVGYLPT
                     MFSGEGGTKEVDLIFEREEP"
     gene            478..846
                     /gene="crcB"
                     /locus_tag="ACZPAQ_05605"
     CDS             478..846
                     /gene="crcB"
                     /locus_tag="ACZPAQ_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR0903496.1"
                     /translation="MWSNCLFVALGGGLGAVGRYLLSLIAAPWSFPVMTLLTNFLGAV
                     VIGAVAQAAGQLPMSKSAVLFLKTGLCGGFTTFSTFSLETVELLEQGRWAAGGGYILA
                     SVGLCLAGVLLGRALVWKIL"
     gene            1000..1434
                     /locus_tag="ACZPAQ_05610"
     CDS             1000..1434
                     /locus_tag="ACZPAQ_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024212.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0903497.1"
                     /translation="MDLRDSLNRYYYDVTVSDLRQISRTGGGELSYNSIMYLDVISYQ
                     RAQGECTVSTLANTLHITKSAVTIKVNELERLGMVTKTRSETDRRVIQLDVSPQMTQS
                     LRAYEGPFERAIRLVERSFTPEQIAVFCSILNTFTSEYIKDF"
     gene            1508..2809
                     /locus_tag="ACZPAQ_05615"
     CDS             1508..2809
                     /locus_tag="ACZPAQ_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903498.1"
                     /translation="MISMAMLSLYTVADGIFVGRYVGPNALAAVNLVMPLIMMSFALV
                     DLVAVGSSVQISIRLGEKNERAANRIFSFCTGLIVALSCVIGLGGFFLAGPMVSLMGA
                     EAEVTAMGAEYMQVYAVFAPVIMVFFAVDNYLRICGRVRYSMILNVVTALLNIVLDFL
                     FLVVFRWGVAAAAAASCLSIALGTVLGFLPFVMGRLPLRFVRGTISARQLGNILANGS
                     SEFFSSIAGSVMMVILNSVLLRLSGSMAVAAFSIVMYVDSIVGSLLFGMADSIQPAIS
                     YNYGARRRDRMLALEKRVLLTGAAVSLAALLWMQLGGQYVIPLFIQGDDPALLEMSLR
                     AMRLFSLSYLLGWAGTCLSSFFTALNRPGRSLILAFSKTLVFPLACLAVLPAALGLDG
                     VWLTAPAAGVLTAALAVWFLTAVLRQERAQDRQEGEGTPQA"
BASE COUNT          552 a          784 c         1030 g          687 t
ORIGIN      
        1 gagtccaacg gtatgctgat ctccgccgtc cacaccgaga agggcgagga gcagctcaac
       61 ctgctgatga tcgacgacaa gatccccgct ggggccaaga tgtgctgaac gggactgtgg
      121 gacagatcga aaaagatctg cgtcatttta atggggcggg cctttggccc gccccatttt
      181 tgtgctatac tgaaagcaga cgccgggggg agaaactggc tccggccccg cctccgggta
      241 ccggaacggg aaagcatctt tgagaaagga aggatggaca tgtaccgata tgaatatgtg
      301 acctatcgca ccgggggcgg cttctggatg gacaacagcg cctgtgagca ccgggagatc
      361 atagacgagt acgcggccaa gggttggcgt tacgtgggat atctgcccac catgttttcc
      421 ggggagggcg gcaccaagga agtggacctg atctttgaac gggaggagcc ctgagccatg
      481 tggtccaact gtttgttcgt ggccctgggg ggcggcctgg gcgccgtggg gaggtatctg
      541 ctcagcctga ttgccgcccc atggtccttt ccggtgatga ccctgctcac caattttctg
      601 ggggcggtgg tcatcggcgc cgtggcccag gcggcgggac agctgcccat gtccaaaagt
      661 gcagttctgt ttttgaagac cggactttgc ggtggtttta ccaccttttc cactttttca
      721 ctggagacgg tggagctgct ggagcagggg agatgggccg ccgggggcgg gtacatcctg
      781 gccagcgtgg gtctgtgcct ggcgggggtg ctgctggggc gggccctggt gtggaagatc
      841 ctttgaggat ggagacccgc atctgagggc ggcgcctcta tccaaatgag ggctgccgcg
      901 gggagaaaat cgtgcggacg gggttgacag agaaaggatc ttctgctata ttagttaagc
      961 aaatttatat aaattcgctt aatgaaatga ggggaacgga tggacctgcg ggatagtctg
     1021 aaccggtatt actacgatgt aacggtcagt gatctgaggc agatcagccg caccggcggc
     1081 ggagagctgt cttataatag catcatgtat ctggatgtga tctcctacca gcgggcccag
     1141 ggggagtgta cggtgagcac cctggccaat accctgcaca tcacaaagtc ggcggtgacc
     1201 atcaaggtca acgagctgga gcggctgggg atggtgacca agacccgcag cgagaccgac
     1261 cggagggtga tccagttgga cgtatccccg cagatgacac agtcgctccg agcctatgag
     1321 ggcccgtttg agcgggcgat ccggctggtg gaaaggagct tcacaccgga acagatcgcg
     1381 gtgttctgtt ccattttgaa tacgtttact tcggaatata taaaggattt ttaaaatgac
     1441 acagcatgaa aaattatttg agagccttcc gcccactcgg ctgtttttcc ggtgcgcctt
     1501 gcccagcatg ataagcatgg cgatgctctc cctgtacaca gtggcggacg gcatctttgt
     1561 ggggcgctat gtgggcccca acgctctggc ggcggtcaat ctggtgatgc cgctgatcat
     1621 gatgagtttc gcactggtgg acctggtggc ggtggggtcc tccgttcaga tctctatccg
     1681 ccttggggaa aaaaatgaga gggcggccaa ccggatcttc agcttctgca ccgggctgat
     1741 tgtggcgctg tcctgcgtca tcgggctggg cggctttttc ctggccgggc cgatggtgtc
     1801 cctgatgggg gcggaggcgg aggtcaccgc catgggggcg gagtatatgc aggtatatgc
     1861 cgtattcgcg ccagtcatta tggtattctt tgcagtggac aactatctgc gcatctgcgg
     1921 ccgggtgcgg tacagcatga tactcaatgt ggttacagca ctgttgaaca tcgtgctgga
     1981 ttttctattt ttggtagttt tccgctgggg ggtggccgcg gcagcggcgg cctcctgtct
     2041 gagcattgcc ctgggtacag tgctgggctt cctgcccttt gtgatgggcc gtctccctct
     2101 gcgttttgtt cgggggacca tctctgcccg tcagctgggg aatattttgg ccaatggcag
     2161 ctcggagttc ttttccagta ttgcgggatc tgtgatgatg gtcattctca acagcgtact
     2221 gctgaggctg tccgggtcta tggcggtggc ggctttctct atcgtgatgt atgtggacag
     2281 catcgtgggc tctctgctgt ttggcatggc agattccatt cagcccgcca tcagctacaa
     2341 ctacggcgcc cggcgccggg acaggatgct ggccctggaa aagagggtgc ttttgacagg
     2401 cgcggcggtg tcgctggcgg ctttgctgtg gatgcagctg gggggacagt atgtcatccc
     2461 tctctttatc cagggggatg accccgccct gctggagatg agcttgcggg ccatgaggct
     2521 gttctccctg tcttatctgc tgggctgggc agggacctgt ctgagctcct tcttcaccgc
     2581 attgaaccgg ccgggacgct ccctgatcct ggccttcagc aagacgctgg tctttccgct
     2641 ggcctgtctg gcggtgctgc cagccgcgct ggggctggac ggcgtgtggc tgacggcgcc
     2701 ggcggccgga gtgctgaccg ccgctctggc agtctggttt ttgaccgcag tgctgcggca
     2761 ggagagagcc caggaccggc aggaggggga gggtacaccc caggcgtgag acccggtccc
     2821 atcatcagaa aaaacaggtc ctgcgctcag aggagcgcag gacctgtttt aatgaatcac
     2881 ggcggttcac gcctcctggg ccatgacggc cagggcctca tccacggcgg agaggaggta
     2941 ctggatgtcc tcctctgtgt gggcggggga gaagcagagg cggctgttgt gcaggatgcc
     3001 gcggtccttc agcagggcca taaagcggct gtgcttgggg aagctggcgt tgg
//
LOCUS       DBMDSJ010000196         3049 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7931_length_3049_cov_1.740841, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000196 DBMDSJ010000000
VERSION     DBMDSJ010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7931_length_3049_cov_1.740841"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="ACZPAQ_05620"
     CDS             <1..368
                     /locus_tag="ACZPAQ_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR0903499.1"
                     /translation="AALSAQIDRIGVITAKIFEYQKSHPERSAQLHAFLSYYLPTTLK
                     ILRAYAQLESQGVEGENIAAAMSRIEGMMGKVVEGFEKQLDQLFQGDAMDITTDVAVL
                     EQMLQKDGLSSSGGMQIGI"
     gene            complement(405..785)
                     /locus_tag="ACZPAQ_05625"
     CDS             complement(405..785)
                     /locus_tag="ACZPAQ_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR0903500.1"
                     /translation="MSVVSGWLIPLLVGAATGVLSGFGVGGGTLLLVYMTTLAGLDQH
                     LAQGINLLYFLPAGLTALPAHLKNGYVEKGLLFPAISAGLLCAALASWAATALDVDLL
                     RKLFGGFLILIGLSELFGRVKEET"
     gene            complement(773..1132)
                     /locus_tag="ACZPAQ_05630"
     CDS             complement(773..1132)
                     /locus_tag="ACZPAQ_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR0903501.1"
                     /translation="MKHRSTWKSVLAGAAAGLTNGFFGGGGGSVLVPMLTRVCGLDQR
                     RAFATSVAVILPLCALSAAVYLLRGGLDLSAALPYLIGGTVGGWAGGRLFKGVRLPWL
                     KRGFGALLIWGGLRCLL"
     gene            complement(1210..1473)
                     /locus_tag="ACZPAQ_05635"
     CDS             complement(1210..1473)
                     /locus_tag="ACZPAQ_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR0903502.1"
                     /translation="MKADHAQVARLLKTARGQIDGILKMVEEDRYCLDVSNQLMATQS
                     ILKKANRLVLKAHMNCCVREAVDSGDPAPKLEELAALLDKLAD"
     gene            complement(1470..>3049)
                     /locus_tag="ACZPAQ_05640"
     CDS             complement(1470..>3049)
                     /locus_tag="ACZPAQ_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-transporting ATPase"
                     /protein_id="HIR0903503.1"
                     /translation="ASSSKAPISKLADKVAGVFVPTVMVIAAIAAGAWWFATGSATSA
                     LTAGVAVLVISCPCALGLATPVAIMVGTGKGAEQGILIKSAEALETLHLVDTVVLDKT
                     GTLTQGRPVVTDILPGEDMDEEGLLILAACLEGPSEHPLAAAIVEESRRRGLPITSVG
                     GFTAVHGRGVRAALGDRTCMGGNRAMMEEAGVELGSFPARAEELAAQGKTPLYFADEH
                     RVLGLVAVADTPKPTSAQAVAAFRRLGIDVIMLTGDNQRTAGAIGAQLGVSQVMAEVL
                     PQDKERKVKQLQDQGKKVAMVGDGINDAPALARADVGLAIGAGTDVAIESADIVLMKS
                     DLLDAAAAVELSKATIRNIKQNLFWAFFYNSLGIPLAAGLFYALGWTDVMLNPMFAAA
                     AMSLSSVCVVTNALRLRFFKPKSVPASPVPCPSGTCPVQPVPDPSEQPTYEPQHQEER
                     KGANETMEKKIMINGMMCAHCTAHVEKALGTLEGVASVKADLEGKCATVTLTGAVSDQ
                     SLTDAVTEAGYEVVSIQ"
BASE COUNT          535 a          978 c         1027 g          509 t
ORIGIN      
        1 acgcggccct ctcggcgcag atcgaccgca tcggggtcat caccgccaag atcttcgagt
       61 atcagaagag ccacccggaa aggtctgccc agctccatgc ctttctgagc tattacctgc
      121 ccacaaccct gaagatcctc cgggcctatg cccagctgga gtcccagggc gtagaggggg
      181 agaacattgc agccgccatg agccggatcg aggggatgat gggcaaggtg gtggaaggct
      241 tcgagaaaca gctggatcag ctctttcagg gggatgctat ggacatcacc accgacgtgg
      301 cggttctgga gcagatgctc cagaaggatg gactgtcctc ctccggcggg atgcagatcg
      361 ggatttgact gattcaaagg ccgcggggat cttccccgcg gccttcacgt ttcttctttt
      421 acccgaccga acagctcgga caggccgatg aggatgagaa agcccccaaa gagctttcgg
      481 agcaggtcca cgtccagggc tgtggcggcc catgaggcca gcgcggcgca gagcaggccg
      541 gcggagatgg cagggaacag aagccccttt tccacatatc cgtttttcag gtgggcgggc
      601 agggcggtga gtccggcggg gagaaagtac agcagattga tgccctgagc caggtgctgg
      661 tccagtccgg ccagggtggt catatagacc agcagaaggg tgccgccccc cacgccgaag
      721 ccggacagga ccccggtagc cgcccccacc agcaggggga tcagccagcc gctcacaaca
      781 gacacctcag gccgccccag atgagcaggg ccccgaagcc ccgcttcagc cacgggagcc
      841 ggacgccctt gaacagccgt cctccggccc agccgcccac agtgccgccg atgagatagg
      901 gcagggcggc ggagagatcc agtccgcccc ggaggagata gacggctgcc gacagggcgc
      961 acaggggcag gatgaccgcc actgaggtgg caaaggcccg ccgctgatcc agtccgcaca
     1021 cccgggtgag catggggacc agcaccgaac cgccgccccc gccgaaaaag ccgttggtca
     1081 gcccggctgc cgccccggca agcacggact tccaggtaga tctatgcttc aaaaaaacgc
     1141 ccccttgccg gtctagtgtg ccaggcaggg gggcgtttta tccattttgc ctgccggggg
     1201 agcgggatct cagtcggcca gcttatccag cagggcggcc agctcctcca gcttgggggc
     1261 ggggtccccg ctgtccacgg cctcccgcac gcagcagttc atgtgtgcct tcagtaccag
     1321 gcggttggct tttttcagaa tggactgggt ggccatcaat tggttggaga cgtccagaca
     1381 gtaccggtcc tcctccacca tcttcaggat accgtcgatc tggccacggg ccgtcttcag
     1441 cagccgggcg acctgggcgt ggtccgcttt cactgaatgg acaccacctc gtatccggcc
     1501 tcagtgactg cgtcggtgag ggactggtcg ctcaccgcac cagtgagggt gactgtggcg
     1561 cacttgccct ccaggtcggc tttgacggag gccaccccct ccagggtgcc cagggctttc
     1621 tccacgtggg cggtgcagtg ggcgcacatc atgccgttaa tcatgatctt cttttccatc
     1681 gtttcattcg ctccttttcg ttcttcctga tgttgtggtt catatgtggg ctgctccgag
     1741 gggtcaggga caggctggac cgggcaggtg ccgctggggc agggaacagg ggaggcaggg
     1801 acgctcttgg gcttgaagaa ccgaagacgc agggcgttgg tcaccacgca taccgagctc
     1861 aggctcatag cggcagcggc gaacatgggg ttgagcatga catccgtcca ccccagggca
     1921 tagaacaggc cggcggccag ggggatgccc aggctgttgt agaaaaaggc ccagaatagg
     1981 ttctgcttga tgttgcggat ggtggccttg gacagttcca ccgcagcggc agcgtccagc
     2041 aggtccgact tcatcagcac gatgtcggcg ctctcgatgg ccacgtcggt gccagcaccg
     2101 atggccaagc ccacgtcggc ccgggccagc gccggggcgt cgttgatgcc gtcccccacc
     2161 atggccacct tcttgccctg atcctggagc tgcttcacct tgcgctcctt gtcctggggg
     2221 agcacctccg ccatcacctg ggagacgccc agctgtgcgc cgatggcgcc ggcggtgcgc
     2281 tggttgtcgc cggtgagcat gatgacgtcg atccccagcc gccggaaggc ggctaccgcc
     2341 tgggcgctgg taggcttggg ggtgtcggcc acagccacca ggccaagcac ccggtgctcg
     2401 tcggcaaagt agaggggggt cttgccctgg gcggccagct cctcggcccg tgcggggaag
     2461 gagcccagct ccaccccggc ctcctccatc atggcgcggt tgccgcccat gcaggtgcgg
     2521 tctcccaggg cggcccggac gccccggccg tggacggcgg tgaacccgcc cactgaggtg
     2581 atggggaggc cacgccttct gctctcctcc acgatagcgg cggccagagg gtgctccgag
     2641 gggccctcca ggcaggcggc caggatgagc aggccctcct cgtccatgtc ctcgcccggc
     2701 aggatgtcgg tgaccaccgg cctgccctgg gtcagggtgc cggtcttgtc cagtaccacg
     2761 gtatccacca gatggagggt ctccagggcc tcagcggatt tgatgaggat gccctgctcc
     2821 gctcccttcc cggtgcccac catgatggcc acgggggtgg ccagacccag ggcgcagggg
     2881 caggaaatga ccagcacggc cacgcccgcg gtgagggcgg aggtggcgct gccagtggcg
     2941 aaccaccagg ccccggcagc gatggcggcg atgaccatga cggtggggac aaagacgccg
     3001 gccaccttgt cggccagctt ggagatgggg gccttggagg aggaggctt
//
LOCUS       DBMDSJ010000197         3037 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7960_length_3037_cov_1.655995, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000197 DBMDSJ010000000
VERSION     DBMDSJ010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7960_length_3037_cov_1.655995"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(16..555)
                     /locus_tag="ACZPAQ_05645"
     CDS             complement(16..555)
                     /locus_tag="ACZPAQ_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR0903504.1"
                     /translation="MAYSLFETAELGPLISKNRTVRSATNEHLSDPSGQITPAWVDSL
                     EALARGGVGIIITGHLSVDRDQRADEGQAVLDQETDPTLLSLAAQRVHQHGALLIAQI
                     SHSGGKARPGVNGRPPLSPGDLTPQLLDRLVEQFRHAARTAQMAGFDGVQVHCAHNYL
                     LSSFLNLRWNHREDAYGGR"
     gene            916..1929
                     /locus_tag="ACZPAQ_05650"
     CDS             916..1929
                     /locus_tag="ACZPAQ_05650"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR0903505.1"
                     /translation="MKRKLFSTLLCAFALLLLMGAGVEQDSAAAAIVIEEESASADGS
                     AQVLETGIQTGIEAQDTEEISADEIYLEAINSDGTQTSVTAEPAVALDHTLLVNGQPV
                     PADTGKTVEQGTTYVALAAMAQTLDPSVTITWDGGSSTAAVQSAVLDLTAKVGQQYLV
                     ANGRYLYVPEGVRMEAGRVVVPLKELVKAFDAKLSWDPADGTTSITTGSGGIASGDSF
                     YNQDDLFWLSRVVYAESGNQSLDGKIAVANVVLNRVKSPIFPNTIEGVLAQKNQFPTY
                     GSGSLAKRTPNEGSIIAAKLAMDGAVVEEAAGALYFDSASNSWASRNKTHITTIGGHK
                     FYG"
     gene            complement(2068..>3037)
                     /locus_tag="ACZPAQ_05655"
     CDS             complement(2068..>3037)
                     /locus_tag="ACZPAQ_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0903506.1"
                     /translation="DRVQLYGMEQFPLDHLTVLEGDLSKLSEPGGRYVAAVYSDDDYG
                     RPEMGSHWARLGDTVTIRYVEEYEYYNPDTGEVYGPWENVPDGANWVDRAVKYRDVEY
                     EVAALVTVPMALSYRYYGADEFIMNDQTFIQDTGTDSVMYYAFDTTDEANAAMESFLA
                     DYTENVNPQFDYESKSTYAAEFEGMRSMFLLLGGALSGIVGLVGVLNFFNAILTGIIT
                     RKREMAVLQSIGMTGKQLKTMLVCEGLLYALSSVLLSLVLAVVLGPLAFSALQSMFWF
                     FTYRFTLAPILAVAPVFALLGALVPLAVYRSISKHTVVERLRESDN"
BASE COUNT          617 a          915 c          965 g          540 t
ORIGIN      
        1 ccactgtgcc cacaattatc tgccgccata ggcatcctcc cggtggttcc accggaggtt
       61 caggaagctg gagagcagat aattgtgggc acagtggacc tggaccccat caaagcccgc
      121 catctgggcc gtccgggccg catggcggaa ctgttccacc agcctgtcca gcagctgggg
      181 cgtgagatcg ccgggggaga ggggcggccg cccgttgacg ccggggcgcg ccttcccgcc
      241 gctgtggctg atctgggcaa tgagcagggc cccgtgctgg tggacccgct gggcggccag
      301 agagaggagg gtggggtccg tctcctgatc cagcaccgcc tgcccctcat ctgcccgctg
      361 gtcccggtcc accgacaggt ggcccgtgat gatgatgccc actccacccc gggccagcgc
      421 ctccagggag tccacccagg cgggagtgat ctgtccactg ggatcggaga ggtgctcgtt
      481 ggtggcggaa cgcacggtcc tgtttttgga tatgagcggg cccagctccg cagtctcgaa
      541 cagcgaatag gccataagaa cgcctccttt tttctcaatt gtacccggaa aaaaggggat
      601 gtcaaggaat tatcagagga aaaggccaaa ggtggacgtg cctggcgggt aggaagacag
      661 cgagtaatgg gcagaaaggc agaagagcgc ggatgtaaaa atgcgtcaga ggagggggaa
      721 agagggctta accggtcatt ctaaggctta tactaggaat attccgaaaa aatgcacaag
      781 atttcagaga gaactctagt caattttgcc cttgcgcctg tttgaggaat tggttacaat
      841 gtggtaacaa aagtaacaaa aacgttgctt ttggtgtcac cgtttcgaca caaattgtaa
      901 tagggagtga agacaatgaa acgcaagctg ttttcaacac tgctgtgcgc atttgccttg
      961 ctcctcctga tgggcgccgg tgtggagcag gactccgccg ccgcggccat tgtgatcgag
     1021 gaggagagcg cctccgccga cggatcggcg caggtgcttg agaccggaat ccagaccggt
     1081 atcgaagcgc aggatacaga agagatctcc gctgacgaga tatatttgga ggcgatcaat
     1141 tccgacggga cccagacctc ggtcacggcc gagcccgctg tggccctgga ccacaccctg
     1201 ctggtgaacg gccagcccgt ccccgcggac acgggaaaga ctgtggagca ggggaccacc
     1261 tatgtggcgc tggccgccat ggctcagacc ctggacccca gcgtgaccat cacctgggat
     1321 gggggctcca gcaccgctgc ggtccagagc gccgttctgg acctgacggc caaggtgggc
     1381 cagcagtatc tggtggccaa cggccgctat ctctatgtcc ccgagggcgt ccgcatggag
     1441 gccgggcggg tagtcgtccc gctgaaggag ctggtcaagg cctttgatgc caagctgagc
     1501 tgggacccgg cagacgggac cacctccatc accacgggga gcggcggcat cgcctccggc
     1561 gactccttct acaaccagga cgacctgttc tggctgtccc gcgtggtcta cgcggagagc
     1621 gggaaccaga gcctggatgg taagatcgcc gtggccaacg tagtcctgaa ccgggtcaag
     1681 agcccgatct tccccaatac catcgaagga gtgctggccc agaagaacca gttccccacc
     1741 tacgggtccg gcagcctggc caagcgcacc cccaacgagg gcagcatcat cgccgccaag
     1801 ctggcgatgg acggcgccgt ggtggaggag gccgccgggg cgctctactt tgactcagct
     1861 tccaacagct gggcttcccg gaacaaaaca catatcacca ccatcggggg ccataagttc
     1921 tatggctgag ccctgatgga caggaggagc cgctcgatcc cggctcctcc tttttgtcat
     1981 ctccccatcc ggtgggccct atggagagat atacaacggg acagctcctc cgcacaggcg
     2041 gaggagctgt cccgttgtat ggtttggtca gttgtccgac tcccgcaggc gctccaccac
     2101 ggtgtgtttg gagatggagc ggtacaccgc cagggggacc agggcgccca gcagggcaaa
     2161 caccggagcc accgccagga tgggggccag ggtgaagcgg taggtgaaga accagaacat
     2221 gctctgaagg gcggagaagg ccagggggcc caggaccacc gccaggacca gggacaggag
     2281 cacagagctc agggcgtaca gcaggccctc acagaccagc atggtcttca gctgctttcc
     2341 ggtcatgccg atggactgga ggacggccat ctcgcgtttc ctggtgatga tgccggtgag
     2401 gatggcgttg aagaagttca gcacccctac caggcccacg atgccgctga gggccccgcc
     2461 cagcagcagg aacatggatc gcatcccctc aaactccgcg gcgtaggtgg acttgctctc
     2521 atagtcgaac tggggattca cattttccgt gtagtccgcc aggaaggact ccatggcggc
     2581 gttggcctca tccgtggtgt cgaaggcata gtacatgacg ctgtccgtgc ccgtgtcctg
     2641 gatgaaggtc tggtcgttca tgatgaactc atccgcgccg taatagcggt agctcagggc
     2701 catgggcacg gtcaccaggg cggccacctc gtactccacg tcccggtact tcaccgcccg
     2761 gtccacccag ttggcaccat cgggcacgtt ctcccatggg ccgtacacct ctccggtgtc
     2821 gggattatag tactcatatt cctccacata ccggatggtg acggtgtccc ccagccgggc
     2881 ccagtgggag cccatctcgg gccggccgta gtcgtcatcg ctgtacacag cggctacata
     2941 ccgcccgccg ggttcactga gcttggacag gtctccctcc agcaccgtca ggtggtccag
     3001 ggggaactgc tccatgccgt agagctgcac ccggtcg
//
LOCUS       DBMDSJ010000198         3036 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7964_length_3036_cov_1.804770, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000198 DBMDSJ010000000
VERSION     DBMDSJ010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_3036_cov_1.804770"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="ACZPAQ_05660"
     CDS             complement(<1..708)
                     /locus_tag="ACZPAQ_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015746.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR0903507.1"
                     /translation="MEMEVKKKGLLERFLRGVEVLGNKIPNPMLLFISLCIIVVLASA
                     ICSFFNVSAVNPVTGETVEVVNLLTKDGFIRMLTNFVTNFTGTSAMGLTLTCMLGVGV
                     AEASGLFQVALRGLAKAKGADLKIIIVFITVCVLADCAGGAGFVVLPPLGAMLWSAMG
                     RNPFAGMFAAYASVTGAFASNILITSMDVINMGFTEAAAQMLVPEISLSPSMNWYFSA
                     VSAVCLVIVSTLVTLKVV"
     gene            1220..2248
                     /locus_tag="ACZPAQ_05665"
     CDS             1220..2248
                     /locus_tag="ACZPAQ_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HIR0903508.1"
                     /translation="MKFAILGAGNTGKAYSAFLSHGGHQVLLYDRSQERLAPIQARGI
                     SATGVVEGRFPVPVTTQLEDCGDSDVILVCTVAAGHRPLAAGLRGVLRPGQIILVTNC
                     CWGAVEFDMELGAEAERKGCPIGETGGQLILCSSPAPDAVYLKTVKKSMGLACVRPED
                     TAGVLERLAPVFPQLCPAANVLETSLNNSNPISHGPLALFNITRMENGEDFLLFGTGV
                     TPRVASFMEKIDRERVAVVRACGVSAPTELEMLNSFWPEPQESLYNVLHNTPAYSVTK
                     GPRTLDHRYLTEDLPYGLVPYVRLGRKLGVETPCLEALIGMFGLYMDQDYFQQGPEVE
                     QLDLSRYR"
     gene            2618..>3036
                     /locus_tag="ACZPAQ_05670"
     CDS             2618..>3036
                     /locus_tag="ACZPAQ_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HIR0903509.1"
                     /translation="MQHEITQAIPLLDEQGELTQPGWARRLLPVYDRSRVRGVPLRLK
                     ERDCYLVMNRQCALALTISDNRYMGMDSISLIDLWEGWQVTRSPMRLMPLGGTGLPQS
                     SLRGSCARAGRGYGLLFQHEGEGRTLHAHMDRFRDGQA"
BASE COUNT          680 a          850 c          956 g          550 t
ORIGIN      
        1 cacgaccttt agggtgacca gggtggagac aatgaccaag cagactgccg ataccgcgga
       61 gaagtaccag ttcatggagg gacttaaaga aatctcggga accaacatct gggcagctgc
      121 ctcggtgaag cccatgttga tcacgtccat ggaggtaatt agaatattgg aagcaaatgc
      181 gccggtgaca gaagcgtagg ccgcaaacat gccagcaaag ggattgcggc ccatggcaga
      241 ccagagcatg gcacccagag ggggaagaac gacaaagcca gcgccgcccg cacagtcggc
      301 cagaacgcac acagtgatga aaacaataat gatcttcagg tcagcgccct tcgccttcgc
      361 caggccgcgc agagcaacct ggaaaaggcc ggaggcctcg gccacgccga cgcccagcat
      421 acaggtcagg gtcagaccca tggcagaggt gccagtgaag ttggtaacaa aattggtcaa
      481 catgcggata aagccatctt tggtgagcag gttgacaacc tctacggtct caccagtgac
      541 ggggtttacc gcgctgacat taaaaaagga gcaaattgca gaggcaagaa cgacgataat
      601 acaaagtgag ataaacagca gcattggatt ggggatcttg tttcctaaga cttcaacacc
      661 ccgtaaaaac cgttccaaca atcctttctt tttgacttcc atttccatag atggatgacc
      721 tccctaagct tttcttgtaa tcacacacat tgcaggggaa tggaagattc cggacggctc
      781 ggcttccgac acggaacctt gtgccgaaaa gggtgggacc ccacttttga acagaatgtt
      841 cccatctgtt catgataaag ctattttagt gccagacaca aactaagtct aacagaaaaa
      901 tgaaaaaatc aacaagcaat tggttgtgac attgaggaag ccataggttt ttcccccaga
      961 gcggcccggg gagctgcaaa aaatgtctaa aaccggcgga taaattttgt atgcttctgc
     1021 atcccatgct tgtggtcagg ctcccagctg gggccggggc tggaaaacat gagggaatat
     1081 gcctggataa ggcggcccgg ggggtgagat cttggatatt ccgcacaaag atgggaagat
     1141 gcacggaaag agaggagttg aaaaattccc cgctttatgc tctaatagaa caaactttca
     1201 aaggtagggg aagcagaata tgaagtttgc gattttggga gcgggaaata ccggaaaggc
     1261 gtacagcgca tttttgtccc acggggggca tcaggtcctt ctctatgacc ggagccagga
     1321 gcggctggcg cccatccagg cccgggggat ctccgccacc ggcgtggtgg aggggcgctt
     1381 ccctgtcccg gtgaccacac agctggagga ctgcggggac agcgacgtga tcctggtgtg
     1441 cacggtggcg gcgggacacc gcccgctggc ggcggggctg aggggcgtgc tgcggccggg
     1501 gcagatcatc ctggtgacca actgctgctg gggcgcggtg gaatttgata tggagctggg
     1561 agcggaggcg gagcggaagg gctgccccat cggggagacg gggggacagc tgatcctgtg
     1621 cagctccccc gcgccggacg cggtctatct gaagaccgtc aaaaagagca tgggactggc
     1681 atgcgtcagg ccggaggata ccgccggggt gctggagcgg ctcgcccctg tattccctca
     1741 gctgtgccct gcggccaatg tgctggagac ctcactgaac aactccaacc ccatctccca
     1801 cgggccgctg gccctgttca acatcacccg gatggagaac ggggaggact tcctgctctt
     1861 cgggacagga gtgaccccca gggtggccag ctttatggag aagatcgaca gggagcgggt
     1921 ggcggtggtc cgcgcctgcg gcgtgtccgc ccccacagag ctggagatgc tcaattcctt
     1981 ctggccggag ccccaggaga gcctgtacaa tgtgctgcac aacacgccgg cctattccgt
     2041 caccaagggc cccaggaccc tggaccaccg gtatcttacg gaggacctgc cctatggcct
     2101 ggtgccctat gtgcgcctgg gccggaagct gggggtggag accccgtgcc tggaggcgct
     2161 gatcgggatg ttcggactgt atatggacca ggattatttc cagcagggac cggaggtgga
     2221 acagctggac ctgtcccggt accggtaagg ccagcggggg ccgcacggat gtgcggcccc
     2281 cgctgcagct tgtcgacccc cccccctccc acgtgaagcc tcgcagagtt ccgtcgcccg
     2341 caggcgacgg aattcaattt aaatccgtca tttccgagga catgagcctc ggaaatgaca
     2401 cttctcatgg cgggcggggc gactatttcg catgaaatcg agtcccacac gccatgcagc
     2461 cctcttctcg aaaaaggcac ttgtgccttt ttcgagagtc tccaacgggg gccgcacgga
     2521 tgtgcggccc ccgttggccc ttccttttcc ctgagatcga tggtaaaata gccctgtgga
     2581 acgccggcgg gactccggcg gaaaggaggc acgactgatg cagcatgaga tcacacaggc
     2641 catcccgctg ctggacgagc agggggagct gacccagccg gggtgggccc ggaggctgct
     2701 gcctgtgtac gaccgctcca gggtgagggg cgtccctctc cgtctgaagg agagggactg
     2761 ctatctggtg atgaaccgcc agtgcgccct ggccctcacc atttcggaca accgctacat
     2821 ggggatggac tccatctccc tgatcgacct ctgggaggga tggcaggtga cccgcagccc
     2881 catgcggctc atgcctctgg gcgggacagg gctgccccag agctctctcc ggggcagctg
     2941 cgccagggcc gggcggggct acggcctgct gttccagcat gagggagagg ggcgcaccct
     3001 ccacgcccat atggaccgct tccgggacgg gcaggc
//
LOCUS       DBMDSJ010000199         3027 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_7992_length_3027_cov_1.718387, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000199 DBMDSJ010000000
VERSION     DBMDSJ010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7992_length_3027_cov_1.718387"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="ACZPAQ_05675"
     CDS             complement(<1..694)
                     /locus_tag="ACZPAQ_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016424.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR0903510.1"
                     /translation="MNFLKKTWVAIALTVIMVVAAIGVGQLRAPVPGLPTDTSNSASG
                     ALDTSLSTSEYDTWISDQAGVLSSSAEEQISLYNANWDYRYNSLIAVLMVDSLDGQDI
                     ESYAYDQGTQIGLGQGDALLVAAVDDELYYVATGNDFSTILTSSVTSRLDGMLSSNFG
                     AQRFEEGVLSFFAEMNQVYIDNFGLGNAGGGGSYQVNERTMGVISLLVLLVAALMVLS
                     AIDRARYNSYRAR"
     gene            complement(731..1936)
                     /locus_tag="ACZPAQ_05680"
     CDS             complement(731..1936)
                     /locus_tag="ACZPAQ_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HIR0903511.1"
                     /translation="MSDNLDMTPQLTLTPEADTAAAAAPAAPEAPTLTLEPAAADPAD
                     EAAAQKERDAHAVKLDESQLTEAERKMVDEFSQKIDITDSSVVLQYGAAAQKNIAGFS
                     ENALNNVRTKDLGEVGEALSSLVVELKGFGKEEDEGGFLGFFKKKRDKLEAMKTSYAK
                     AEVNVDKIVRVLENHQVVLMKDIAMLDQMYDLNTKYYKELTMYIIAGKKRLEHVRAHD
                     LEELKKKAAETGAQEDAQKYNDYANLCNRFEKKLHDLELTRMISIQMGPQTRLIQNND
                     TLMLEKIQSSLVNTIPLWKSQMVLALGLEHSRQATAAQSAVTDMTNQLLQKNADLLKM
                     GTIETARESERSVVDIQTLQHTNQQLISTLDEVMKIQQEGSQKRKEAELELGRIEGEL
                     KQKLLELRG"
     gene            complement(2100..2804)
                     /locus_tag="ACZPAQ_05685"
     CDS             complement(2100..2804)
                     /locus_tag="ACZPAQ_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HIR0903512.1"
                     /translation="MAKHVKRSVVPIYLVGAVWLAYALLFPLYQPVHYLLCGALSAAA
                     FIVGKGLFPNKVYEVPGEPEENKQEEPEKKEPPKSTGDPEIDALIKERDRAVGEMRRL
                     NDSIEDEEISAQIDHLEEVTRKIIDLVVKQPKKLPQIRRFLDYYLPTTLKILNAYDRM
                     DAAGISGENIDATKKKVEQMMATISQAFDRQLDALFGDEALDISTDITVMEQMLQREG
                     LGGQKKDGDGGVTLEL"
BASE COUNT          513 a          980 c          831 g          703 t
ORIGIN      
        1 aacgtgcccg gtagctgttg tagcgcgccc ggtcgatggc ggagagcacc atcagggccg
       61 ccaccagaag gaccagcagg gagatcaccc ccatggtccg ctcattgacc tggtagctcc
      121 ctccccctcc ggcgttgccc agtccgaagt tgtctatata tacctggttc atctccgcaa
      181 agaaggacag cacaccctct tcaaagcgct gggcaccgaa gttgctggaa agcatcccat
      241 ccagccggga agtcacactg ctggtcagga tggtggagaa gtcgttgcct gtagccacgt
      301 aatagagctc gtcatccacc gcagccacca gcagagcgtc gccctggccc aggccgatct
      361 gggtcccctg gtcgtaagca tagctttcga tgtcctgccc atccaggctg tccaccatca
      421 gtacggcgat cagactgttg taccgatagt cccagttggc gttatagagg ctgatctgct
      481 cctctgcgga ggaggacagc accccggcct gatctgagat ccaggtgtca tactccgacg
      541 tggacaggga ggtgtccagc gcgccggagg cactgttgga tgtgtctgtg ggcagtccgg
      601 ggacgggagc ccgcagctga cccacgccga tagccgccac gaccatgatc acagtcagcg
      661 ctatggccac ccaagtcttt ttcaaaaagt tcatcgtttc acctcacgca gcgggacagg
      721 accgccggcc tcagcctctc agctccagca gcttctgctt cagctcgccc tcaatgcggc
      781 ccagctccag ctcggcctcc ttccgcttct ggctgccctc ctgctggatc ttcatcacct
      841 cgtccagggt ggaaatcagc tgctggttgg tgtgctggag ggtctggata tccaccacgg
      901 agcgctcaga ttcccgggcg gtctcgatag tgcccatctt cagcaggtcc gcgttcttct
      961 ggagcagctg gttggtcatg tcggtaactg cggactgggc ggccgtggcc tggcggctgt
     1021 gctccagccc cagggccagc accatctggc tcttccacag ggggatggtg ttgaccaggg
     1081 aggactggat cttctccagc atcagggtgt cgttgttctg gatcagccga gtctgagggc
     1141 ccatctggat ggagatcatc cgggtgagct ccaaatcgtg gagcttcttc tcaaagcggt
     1201 tacacaggtt ggcatagtcg ttgtatttct gtgcgtcctc ctgggccccg gtctcggcgg
     1261 ccttcttctt cagctcctcc aagtcgtggg cccggacatg ctccagccgc tttttccccg
     1321 cgatgatata catagtcagc tctttgtagt atttggtgtt gaggtcatac atctggtcca
     1381 acatggcgat gtccttcatc agcaccacct ggtggttctc cagcacccgc acgatcttgt
     1441 ccacgttgac ctcggccttg gcgtagctcg tcttcatggc ctccagttta tcccgcttct
     1501 ttttgaagaa gcccaggaag ccgccctcgt cttcctcctt gccgaagccc ttcagttcca
     1561 ccactagaga ggacagggcc tcccctacct cgcctagatc cttggtccgc acgttgttca
     1621 gtgcgttttc cgagaagccg gcgatgttct tctgggcggc ggcgccgtac tggagcacca
     1681 cgctggagtc ggtgatgtcg atcttctggg agaactcatc caccatcttc cgttccgcct
     1741 ccgtgagctg gctctcatcc agcttgacgg cgtgagcgtc ccgctccttc tgggctgcgg
     1801 cctcgtcagc cgggtccgcc gctgcaggct cgagggtcaa ggtgggggcc tccggggcgg
     1861 ccggtgccgc ggccgcagcg gtgtccgcct ccggcgtcag agtcagctga ggggtcatat
     1921 ccagattgtc gctcattgtc gtttcctcct gtatcgtcac gtttctgatc tcattgtcct
     1981 agatatcacg ggacctcggt ccccgctttc cagaagtcat atcccgtcag cacggccatc
     2041 tccatcgccc catgatcagt ttctccacct gttccacggc ctcccccact cttctcggct
     2101 cacagctcca gggtcacgcc tccgtctccg tccttcttct ggccgcccag cccctcccgc
     2161 tggagcatct gctccatgac ggtgatatcg gtagagatgt ccagtgcctc atccccgaac
     2221 agggcgtcca gctgcctgtc aaaagcctgg gagatggtag ccatcatctg ctccaccttc
     2281 ttcttggtgg cgtcgatatt ttcccccgag atgccggccg catccatacg gtcataggca
     2341 ttcaggatct tcagggtggt gggcagatag taatccagaa accggcggat ctggggcagc
     2401 tttttaggct gcttcaccac caggtcgatg atcttccggg tgacctcctc cagatgatcg
     2461 atctgggcgg atatctcctc gtcctcgatg ctgtcgttga ggcggcgcat ctcacccacc
     2521 gcccggtccc gctctttgat gagggcgtcg atctccggat cgccggtact tttaggaggt
     2581 tccttcttct cgggctcctc ctgtttgttc tcctccggct ctccgggcac ctcatacacc
     2641 ttattgggga agagtccctt ccccacgatg aaggccgccg ccgacagcgc cccgcacagc
     2701 aggtaatgga ccggctggta cagtgggaac agcagcgcat aggccagcca gaccgcaccc
     2761 accagataga tggggaccac cgaccgcttt acatgttttg ccatatctga acttcctcca
     2821 cttccgcagg ccggcccctt ccccggtccg gcctgcaaag ctgccttcat atgtcctgtt
     2881 gtgagatatt gtacccccac ggagatgggg tgtcaaggaa attagccgca acttccggcc
     2941 ggctgtccat tatcattaaa cgcacagaga ctcaactgag tcggctgtgc gttttttctt
     3001 tactacaacc ccataggacg gaggtga
//
LOCUS       DBMDSJ010000200         3020 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8012_length_3020_cov_1.919836, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000200 DBMDSJ010000000
VERSION     DBMDSJ010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8012_length_3020_cov_1.919836"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="ACZPAQ_05690"
     CDS             complement(<1..472)
                     /locus_tag="ACZPAQ_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR0903513.1"
                     /translation="MVFLKRFSARDLIFYLLAILVMVFTFNAVQQMNEPDDPTYNEIR
                     LLFEQEKVKYFSVEDNVLTLTLRGEGEDTSTLKYHLADFNVFYNDLHELIDSQRQAGI
                     IQDYDYPAGWAEPWWFVYVPYLVILLGFGILMYTMYLRQNTGGGGGGGPSRFGHA"
     gene            complement(530..1072)
                     /gene="hpt"
                     /locus_tag="ACZPAQ_05695"
     CDS             complement(530..1072)
                     /gene="hpt"
                     /locus_tag="ACZPAQ_05695"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR0903514.1"
                     /translation="MLEKDIQEILFSEEQLAQRVKEIAGEINRDYVGQEIMLVSVLRG
                     SFVFMADLCRRIDLPCTVDFMAVSSYGGGTSSSGQVQITKDLSSDITGKNIIVVEDIL
                     DSGNTLSYLLKVLEQRSPASIRLCTLLDKPERRVKPVEVHYSGFTIPDAFVVGYGLDY
                     AEHYRNLPYIGILKPEVYGG"
     gene            complement(1112..2476)
                     /gene="tilS"
                     /locus_tag="ACZPAQ_05700"
                     /pseudo
     CDS             complement(1112..2476)
                     /gene="tilS"
                     /locus_tag="ACZPAQ_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
     gene            complement(2479..>3020)
                     /locus_tag="ACZPAQ_05705"
     CDS             complement(2479..>3020)
                     /locus_tag="ACZPAQ_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HIR0903515.1"
                     /translation="ESDWEKVAAAADALNRTKILIDDDSTVSVADINAKCRRVEDLGL
                     VVIDYLQLMTSAGGKERSGDNRQQIVSDISRALKIMAKELNVPVLCLSQLSRANESRT
                     NKRPMLSDLRESGAIEQDADIVMFLYREDYYEKESENHNLAECIIAKNRHGETRTVEL
                     QWLPEYTTFSSIDRTHEEY"
BASE COUNT          505 a         1024 c          944 g          547 t
ORIGIN      
        1 gggcatggcc gaagcgggac ggcccccctc ctccgccgcc cccagtgttc tgccgcagat
       61 acatggtgta catcaggatg ccgaagccca gcaggatcac caggtagggc acatagacga
      121 accaccaagg ctccgcccag ccggcggggt agtcatagtc ctggatgatc ccagcctggc
      181 gctggctgtc gataagctcg tgcaggtcgt tatagaacac attgaagtcg gccaggtggt
      241 acttcagggt ggaggtgtcc tccccctccc cccgcagagt cagagtgagc acattgtcct
      301 ccacggagaa gtacttcacc ttctcctgct caaagagcag gcggatctca ttataagtgg
      361 ggtcatccgg ctcgttcatc tgctgcaccg cattgaaggt gaacaccatc accaggatgg
      421 ccagcagata gaagatcagg tcccgcgcgc tgaaccgttt caggaaaacc acgtcctttc
      481 cggcggatca caaatttcag gaggccgccg ccctccaagt ctctccggct cagccgccgt
      541 agacctccgg cttcaggatg ccgatgtagg gcaggttccg gtaatgctct gcgtaatcca
      601 gcccatagcc caccacaaag gcgtctggaa tggtgaagcc ggagtagtgg acctccactg
      661 gcttgacccg gcgctccggc ttgtccagca gggtacacag ccggatggag gccgggctgc
      721 gctgctctag caccttcagc agatagctca gggtgtttcc gctgtccagg atatcctcca
      781 ccacgatgat gttcttcccc gtgatgtcgc tggacaggtc cttggtgatc tgcacctggc
      841 cgctggagga ggtcccgccg ccataggagg acaccgccat gaagtccacc gtacagggca
      901 gatcgatgcg ccggcacagg tccgccatga acacaaagga acctcggagc acgctgacca
      961 gcatgatctc ctgtccgacg tagtcccggt tgatctcacc ggcgatctcc ttcacccgct
     1021 gggccagctg ttcctctgaa aacagtattt cctggatatc cttttccaac atgaaatatt
     1081 ctccttttct tttcgccgta atatgccagc agggctcccc caggcggggc agaaaggccg
     1141 cgtccggccc cagctccgcc accgcagcca ccctgccgcc gctgtccagc acagggacgg
     1201 tatcccgcag gtgcctgggc agcttctggt cgatcatgat ttttttcacg gttcggcccg
     1261 gacggcccgg acgctccagc cggtccccgg tcctccgggg acggacggtg agcccctctg
     1321 ccccagccag ccagaattcc cggcccgact ggggctcccc ttggtacacc gctgccgcgc
     1381 aggtcagggt ccactccccc gtccgcagct gcccgggcat ggggagggat gcctcctcca
     1441 gtgccggggg cgcagtctcc cgggtgagca ccagcatctc atactcccgt cgggcggtga
     1501 tgccctccgg cagatccagc cgggcagagg ggtccctccc ccggcacagg gccaccaccc
     1561 cccccagatg gggggcggcc agatcggtat ctccgtcccg caggcgggcc agcagcagcc
     1621 gggccgcccg gggcgccaag gcgtcaggca gatcccccac cgctgccgcg ggcaggcgga
     1681 gggtccccgc gcgggggagg aggtccgcca ccgccttttc cgccaggccg gtgaggtagt
     1741 cctcgtctgt ccgcagcagg gctgcggtct ccgccgtccg tctggcgaag ccggggcaca
     1801 tgtcctccag ctccggcacc acctgccagc gcaggcggtt gcgggaaaag gactcgtccc
     1861 tgttggagct gtcctccaca tggggcagtc cgtagaggcg cagatattcc tccacctggg
     1921 cccgtgtggt ggtgagcatg gggcggatca gcccctcccc caccgggcgg atgccggtca
     1981 gcccccgcag gccgctgccc cggagcaggt ggagcaggat ggtctctcca ttgtcatccg
     2041 cggtgtgggc ggtggcgatc agggctccgc ccagctctgc cgcggtctcc cggaggaagg
     2101 cgtaacgcat ctcccgggcg gtctcctcca ggcctcgtcc cagctctccg gcctgggcag
     2161 ccacgtcgcc ccggcccaca tagaggggga caccgggcag ctcatgtccg tccacctggg
     2221 cggggccgca ccactgggcg acgaagcggc ggacgaatgc ttcgtcccgc cgggattcct
     2281 ccccccgcag ctgatggttg tagtgggcgg cagccagacg gaaccccatc cgctcccgga
     2341 gcaggtacag ccggtgcagg agatacaccg agtccgcccc cccagagcag gcgcacagca
     2401 ccgtgctccc cggcgggatc attccgtagg tctcgatgag ggcgttcagc ccatggaacg
     2461 ggctggaatg ggccatggtc aatactcctc atgggtccgg tcgatggagg agaaggtggt
     2521 gtactcaggc agccactgga gctccaccgt ccgggtctcc ccgtgccggt ttttggcgat
     2581 gatgcactcg gccaggttgt ggttttctga ttctttctcg tagtagtcct cccggtagag
     2641 gaacatgacg atatcggcgt cctgctcgat ggcccccgat tcccgcagat ctgagagcat
     2701 gggccgctta ttggtccggc tctcgttggc acgggagagc tgagacaggc acagcaccgg
     2761 cacgttcagt tccttggcca tgatcttcag ggcccgagag atgtcggaga cgatctgctg
     2821 ccggttgtcg ccgctgcgct cctttccgcc ggcggaggtc atcagctgga ggtagtcgat
     2881 gaccaccagc cccaggtcct ccacccggcg gcatttggcg ttgatatcgg ccaccgacac
     2941 cgtagagtcg tcgtcgatga ggatcttagt gcggttcagg gcgtcggcgg cggcggccac
     3001 tttctcccag tcgctctcat
//
LOCUS       DBMDSJ010000201         3016 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8022_length_3016_cov_1.505660, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000201 DBMDSJ010000000
VERSION     DBMDSJ010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8022_length_3016_cov_1.505660"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(384..731)
                     /locus_tag="ACZPAQ_05710"
     CDS             complement(384..731)
                     /locus_tag="ACZPAQ_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903516.1"
                     /translation="MNLGSLLLGLTAWGIPAVYFSRRKPGGDRAGLACLLSMASCAGA
                     LFLQVLYNKHLVELQDWSALMDTSGAVAWVSALLLAVTLILDLFCLWAEGKLNGEERG
                     KGGPEEPEIRREA"
     gene            complement(950..1387)
                     /locus_tag="ACZPAQ_05715"
     CDS             complement(950..1387)
                     /locus_tag="ACZPAQ_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903517.1"
                     /translation="MNAAEMKRAAGRALELGLTAVLTVPVVLVILAAAAAVVWLWASR
                     MLPQPVYEPETFTQARWEELDDRGVLVDSLLEQYDGLEGMDLAEVQALLGEHPYTRED
                     PEVGMYRDGYYIGAGRENSGREEVMVLQFRDGEVIRVEFEDAG"
     gene            complement(1409..1942)
                     /locus_tag="ACZPAQ_05720"
     CDS             complement(1409..1942)
                     /locus_tag="ACZPAQ_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903518.1"
                     /translation="MDTVSWLSFFTASGLGAALGVLSLTALGACLYLRAPGQRRLAIL
                     AAALGCVLVLQYGGGALLEERGLFWRGWVQTALSLTGGGLAVLLLAGTIRGLGALCLG
                     KDWVTAVCVLGCAAALLGAAACAGAGLVGSGGREQVVRLAGETLVVRDADLLHQVGWR
                     YVNLFVHGEPLACWRVS"
     gene            complement(1948..2409)
                     /locus_tag="ACZPAQ_05725"
     CDS             complement(1948..2409)
                     /locus_tag="ACZPAQ_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903519.1"
                     /translation="MCGRTETVERRRMVRTLAGAAAVLTVRYGGGFLLALAGLGWRCW
                     VRNLMVLALCVLLFQGIWYGLQALGERPRVRGRWPEIVLGLAGGAALLTLAPFLLLFV
                     FFLRDYDRVSEWEGQAVVAEYRGIDRGSHQCPRVCRYESWLLRGEELGPGR"
     gene            complement(2393..2902)
                     /locus_tag="ACZPAQ_05730"
     CDS             complement(2393..2902)
                     /locus_tag="ACZPAQ_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903520.1"
                     /translation="MPVVMLLLILMPLTMAGELCMLCQCLFLGRGRARRRLALLAGAV
                     LLLLAVWLGGGALLETAGLGWRRWVSGGLVAAAAIAAAALALLLPRELHTLLPGAESL
                     GRICSYLTVLVLAACLGLLSMLGLWRDRVREWEGQTVVAEYSGIFRETGYRYVNWFVH
                     GEQVYVWED"
BASE COUNT          514 a         1217 c          831 g          454 t
ORIGIN      
        1 tcctccgttc cgtaggccag gatgccccgg tggttctcga tccgcagctc ccggtccccc
       61 accagctcca ccttgggcag tccggccagg gcgtccgcag gcagatcgaa cagctctgcc
      121 gtcttttcca aaagcccctc ccggcgcggt tttttctcca tggcgcatca cctctgctta
      181 tgtgtatgag ggaggatggg aaaagttgta cccgattttt ccgcgccggc cccgccccag
      241 cagggcgggc agtcccggac aggaacagcc gccgcccggt ttccgggcgg cggctgtcct
      301 cagcagtccc acggggctgc tggccgtctt tttctctctg ttcatgggat cggcggcgcg
      361 ggatgggcgg ccggagcgcc gcctcacgcc tcccttcgta tctccggctc ctccgggccg
      421 cccttgcccc gctcctcccc attcagcttt ccctccgccc acaggcagaa caggtcaagg
      481 atcagcgtca ccgccagcag gagggcggac acccaggcca ccgctccgct ggtgtccatc
      541 agggcggacc agtcctggag ctccaccaga tgcttgttgt agaggacctg caaaaacagg
      601 gccccggcgc aggacgccat gctgaggaga caggccagcc ctgcccggtc cccgcccggc
      661 tttctccggg agaaatacac cgctgggatg ccccaggcgg tcaggcccag cagcaggctc
      721 cccaagttca tccatccgta ccccacttca ttcgtccttt ctgtgtcctg gcgcttttgt
      781 cctcccatag gtctccccca aaggggcggc cgtttcttcg gggcccgttt ttctccgcag
      841 ggtccaattc cctcatcggg ccctgtgcac cgccctccgg cctctgtgta ggggcggggc
      901 ctgcccctcc gtcccattgg accggtccgg cggaaggcgc cccctccggt cagccggcgt
      961 cttcaaactc cacccggatc acttccccgt cccggaactg gagcaccatg acctcctccc
     1021 ggccgctgtt ctcccggccc gcgccgatgt agtagccgtc ccggtacatc cccacctccg
     1081 ggtcctcccg ggtatagggg tgctccccca gcagggcctg cacctccgcc aggtccatgc
     1141 cctccagccc gtcgtactgc tccagtaggc tgtctaccag cacgccccgg tcatccaact
     1201 cctcccaccg ggcctgggtg aaggtctccg gctcatagac cggctggggc agcatgcggc
     1261 tcgcccacag ccacaccacc gccgccgcgg ccgccagtat gaccaggacc acggggacgg
     1321 tcagcactgc cgtcagcccc agttccagag cccggcccgc cgccctcttc atttccgctg
     1381 cgttcatcgc tgtgttcctc ctctcttttt atgagacccg ccaacaggcc aggggctctc
     1441 catgcacgaa caaatttaca taacgccatc ccacctggtg gagcagatcc gcatcccgga
     1501 ccaccagggt ctctcccgcc aggcggacca cctgttctct cccgccgctt cccacaagcc
     1561 ccgctccggc gcaggcggcc gcccccagca gggctgccgc acagcccagg acgcacaccg
     1621 ctgtcaccca gtctttcccc aggcagagcg cccccaggcc ccggatggtc cctgccagca
     1681 ggaggaccgc cagcccgccc ccggtcagag acagggcggt ctggacccac ccccgccaga
     1741 agagcccccg ttcttccagc agggcaccgc ccccatactg gaggaccagc acacagccca
     1801 gcgccgcggc caggatggcc agccgcctct gccctggggc tctcagatac aggcaggccc
     1861 ccagcgccgt cagggacagc acccccaggg ccgcccccag gccggaggcg gtaaaaaagc
     1921 tgagccagct cacagtgtcc atccccgtca cctcccgggc cccagctcct ccccccggag
     1981 gagccagctt tcatatcggc agaccctggg gcactgatgg ctcccccggt cgattcccct
     2041 gtactccgcc accacagcct gtccctccca ctcgctgact cggtcataat cccgcaggaa
     2101 aaagacgaac aggagaagga agggcgccag cgtcagcagg gccgcgccgc cggccagacc
     2161 caggacgatc tccggccagc gcccccggac ccgggggcgc tcccccaggg cctggagccc
     2221 ataccagatc ccctggaaga ggaggacgca cagtgccagc accatcaggt ttcggaccca
     2281 gcaccgccag cccaggcccg ccagcgccag caggaagccg cccccgtacc ggactgtcag
     2341 gaccgccgcc gcccccgcca gggtccggac catccgccgc cgctccactg tctcagtcct
     2401 cccacacata gacctgctcc ccgtggacga accagtttac ataacggtat ccagtctccc
     2461 ggaaaatgcc gctgtactcc gccaccacgg tctgcccctc ccactccctg acccgatccc
     2521 tccacagccc cagcatggac agcagcccaa gacaggccgc cagcaccagc acagtcagat
     2581 aggagcagat cctgcccagg ctctctgccc ccgggagcag cgtatgcagc tcccggggca
     2641 ggaggagggc cagcgcagcc gcggcgatgg cagctgccgc caccagtccg ccggacaccc
     2701 accgccgcca gcccaggccc gccgtctcca gcagggcgcc gccccccagc cagaccgcca
     2761 gcaggagcag gacggccccg gccagaaggg ccagccttcg gcgcgcccgg ccccgcccca
     2821 gaaacagaca ctggcacagc atgcacagct cccccgccat ggtcaggggc atcaggatca
     2881 aaagcagcat caccacaggc atgtgtttcc tccatagttt accatattat aatttatgtt
     2941 accttaaagc gcacaccggg ccgcgccgtt ggagcggtca gaaccgcatc tcaaagcaga
     3001 accacccggg ctccag
//
LOCUS       DBMDSJ010000202         2988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8106_length_2988_cov_1.541046, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000202 DBMDSJ010000000
VERSION     DBMDSJ010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8106_length_2988_cov_1.541046"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="ACZPAQ_05735"
     CDS             <1..1092
                     /locus_tag="ACZPAQ_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019561.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR0903521.1"
                     /translation="TGETILESQGLTIAAVSDTAVDLKITAPISILNKLSNRNLSVTV
                     NVSQCTEGEQILQYDVNWPRDVSTAEITKESQEPAAITVMVARLYSKTFEIQPVLKAS
                     IAPGYQAGQITISPETVTISGSEEAVSRVAKVEAVLEGEELNTRFSGNVPLVLLDAEG
                     NELTDLEINMDTDTTYVTMPVYVVKEVKLTVDYTPGGGATGEDVTSAIIFPDSITIAG
                     SEEDVSKVEEISLGSIDLSKVVGTNSFTLPIELDSSLENVSGITEAVVTVTISGLSTR
                     TFNVTNIDYDNAPEGYHVEVATESCSVVVRGSEERLEDISASQLRIVADLSNVMTVGS
                     VSVPARVYLDYNSEVGVIGEYSVVVNISR"
     gene            1135..1560
                     /locus_tag="ACZPAQ_05740"
     CDS             1135..1560
                     /locus_tag="ACZPAQ_05740"
                     /inference="COORDINATES: protein motif:HMM:NF016160.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HIR0903522.1"
                     /translation="MSQNAIVKKVVHPGVVEVSLMRQMECGLSCSSCEGCPQRPKEEI
                     LALASDPQGGAKPGDWVEVEPASGSAIGFALLVFFLPCVFLLGGYLIGRLLGLGDGGS
                     IGMAAAGLVLGFVPAVLINRTITRRKTPEFTVLGYLHSV"
     gene            1571..2443
                     /locus_tag="ACZPAQ_05745"
     CDS             1571..2443
                     /locus_tag="ACZPAQ_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR0903523.1"
                     /translation="MFGYVRPVREELKCKDFDLYRATYCGLCRELRRKYGLLAPMFLN
                     FDFTFLALLLWPAEQRFVPCRGRCHANPLARKEMCPADPALEAAADESIILTYWKLRD
                     SARDDGPFSGLPARGLALLLAPAYRKAAVCRPEFDRTVRSCLEELTGLEAENCPSIDR
                     TADTFARILQAAAPPSEDRSRDRALEQLLYHLGRWIYLIDARDDLEEDRASGCYNPVR
                     ARYGEGGDDQALALTLEHSLNLARSAFCVPDFGCRTPVLENILYLGLPMIQQAVFDGS
                     WSRLKKEKIWRKPT"
     gene            2440..>2988
                     /locus_tag="ACZPAQ_05750"
     CDS             2440..>2988
                     /locus_tag="ACZPAQ_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR0903524.1"
                     /translation="MNDPYSVLGVSPNASDEEIKKAYRELARKYHPDNYQNNPLADLA
                     EEKMKEINEAYETITKQRSGGGGSYQRPSGGYGGQSYGGGYQYSHQQSGSANPTYARI
                     RNLINSGDLGTAERLLNEVPQKNGEWYFLSGSIAYRKGWLDEAMQNYSLACQMDPGNM
                     EYRQALAMMQQGGQAYRPYGYSG"
BASE COUNT          614 a          877 c          939 g          558 t
ORIGIN      
        1 acgggagaga ctatcctgga gagtcagggg ctcactatcg ctgcggtctc tgacacggcg
       61 gtggacctga agatcaccgc ccctatcagt atcctgaaca agctgagcaa ccgcaatctc
      121 agcgtgaccg tgaacgtgtc ccagtgtacc gagggggagc agatcctcca gtatgacgtg
      181 aactggcccc gggacgtgag cacagcggag atcaccaagg agtctcagga gcctgccgct
      241 atcaccgtta tggtggcacg gctgtactcc aagacctttg agatccagcc cgtgctgaag
      301 gccagtatcg ccccgggcta ccaggcgggc cagatcacca tcagtccaga gaccgtgacc
      361 atcagtggct cggaggaggc ggtctccagg gtggcgaagg tggaggcggt gctggagggc
      421 gaagagctca acacccgctt ctccggcaat gttcctctcg ttctgctgga cgcagagggg
      481 aacgagctga cggatctgga gatcaacatg gacacagata ccacctatgt gaccatgccg
      541 gtctatgtgg tgaaggaagt taagctgacc gtagactaca cgccgggcgg cggtgccacc
      601 ggcgaggatg tgacctcggc catcatcttc ccggactcta tcaccatcgc cggctcagag
      661 gaggacgtgt ccaaggtaga ggagatctcg ctgggcagta tcgacctgtc caaagtggtg
      721 gggaccaaca gcttcaccct acccatcgag ctggactcca gcctggagaa cgtcagtggc
      781 atcacagagg ccgtcgtcac ggtgaccata tccggcctgt ccacccgtac cttcaatgtg
      841 accaacatcg attatgacaa tgcaccggag ggctatcatg tggaggtggc taccgagtcc
      901 tgctctgtgg tggtgcgggg cagcgaggag cgcctggagg acatctctgc cagccagctg
      961 cgcatcgtgg cagacctgtc caatgtgatg acggtgggct ccgtctccgt cccagccaga
     1021 gtgtatctgg actataacag tgaggtaggc gtcattggag agtactctgt tgtggtcaac
     1081 atcagcagat agtaaaaacg gtttttgagg caaaactttg gaggcagcag catcatgagt
     1141 caaaatgcca ttgtaaaaaa agtggtccat cccggtgtgg tggaggtgtc ccttatgcgc
     1201 caaatggagt gcggcctgag ctgttccagc tgcgagggct gcccccagcg ccccaaggag
     1261 gaaatcctgg ccctggcctc tgatccgcag gggggggcca agccggggga ctgggtggag
     1321 gtggagcccg cctccggcag cgccatcggc ttcgcccttc tggtcttttt cctcccctgc
     1381 gtctttcttc tggggggata cctcatcggc aggcttctgg gtctgggaga cgggggttcc
     1441 atcggaatgg cagccgcggg gctggtcctg ggcttcgttc cggcggtcct gatcaaccgg
     1501 accatcaccc ggcggaagac cccggaattc accgtgctgg ggtatcttca cagcgtgtga
     1561 ggagagccct gtgtttggat atgtccggcc tgtccgggag gaactgaagt gtaaagattt
     1621 tgacctttat cgtgccacat actgtgggct ctgccgggaa ctgcggcgga aatacggcct
     1681 gctggcaccc atgttcctca attttgattt cacctttctg gccctgttgc tctggccggc
     1741 agaacagcgg ttcgtcccct gccgcggcag gtgccacgcc aatccgctgg ccagaaagga
     1801 gatgtgtccc gctgatccgg ccctggaggc ggcggcggac gagagcatca tcctaaccta
     1861 ctggaagctg cgggattctg ctcgggacga cggccccttc agcggcctgc cagccagagg
     1921 gctggccctg ctcctagctc ccgcctaccg aaaggcggcg gtctgccggc cggaatttga
     1981 ccggacagtc cgttcctgcc tggaggagct gacagggctg gaggcagaaa actgcccctc
     2041 aatcgaccgg acggccgaca ccttcgcccg catcctccag gcagccgcgc ccccctctga
     2101 ggaccggagc cgggatcggg cgctggagca gctcctctac cacttggggc ggtggatcta
     2161 tctcatcgac gcccgggacg atctggagga ggaccgcgcc tccggctgct ataaccctgt
     2221 ccgcgcccga tatggggagg gaggggacga ccaggcgctg gccctgactc tggagcactc
     2281 cctaaatttg gcccgctccg cgttctgcgt accggacttt ggctgccgaa cccctgtgct
     2341 ggagaatatc ctctatttgg ggctccccat gatccagcag gccgtctttg atggcagctg
     2401 gtcccgtctg aaaaaagaaa agatttggag aaaacctaca tgaacgatcc gtacagtgtt
     2461 ttgggggtca gccccaatgc cagcgatgaa gagatcaaaa aggcctatcg tgagctcgcc
     2521 cggaagtacc atccggacaa ttatcagaac aatcccctgg ccgatctggc tgaggagaag
     2581 atgaaagaga tcaacgaggc ctatgagacc atcaccaagc agcgcagcgg aggtgggggc
     2641 tcttatcaac gtccgtccgg cggatacggc gggcagagct atggcggcgg ataccaatac
     2701 agtcatcaac agagcggctc cgccaaccct acctacgccc gcatccggaa cctgatcaac
     2761 agcggagatc tggggacggc ggagcgcctg ctcaacgagg tgccccagaa aaacggggag
     2821 tggtatttcc tttccggcag catcgcctac cggaagggct ggctggatga ggcgatgcag
     2881 aactactccc tggcctgcca gatggaccca gggaacatgg agtaccgcca ggccctggcc
     2941 atgatgcagc aggggggaca ggcataccgc ccctacggat acagcgga
//
LOCUS       DBMDSJ010000203         2987 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8109_length_2987_cov_1.541927, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000203 DBMDSJ010000000
VERSION     DBMDSJ010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8109_length_2987_cov_1.541927"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            240..440
                     /locus_tag="ACZPAQ_05755"
     CDS             240..440
                     /locus_tag="ACZPAQ_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903525.1"
                     /translation="MDDFWIERAPEKLSSISRTIRLDAALFERIMEESEKTGVSFNKI
                     VNQCITYAFQHYREPKVTPDGR"
     gene            430..870
                     /locus_tag="ACZPAQ_05760"
     CDS             430..870
                     /locus_tag="ACZPAQ_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903526.1"
                     /translation="MEDKTILYSVRYPSAQEEGERIKVRGDRQAAEAKLTEFLRRMRE
                     ERTYEPIPGREEKGRRFAALARELSQIYEVGLDILRTPDSIEAALHLYCSAYSRDMTR
                     RLAELLGMCDVCAASRPRTEPSDLTLSLMLYTHRCYVSGRLISG"
     gene            complement(930..1961)
                     /gene="mreB"
                     /locus_tag="ACZPAQ_05765"
     CDS             complement(930..1961)
                     /gene="mreB"
                     /locus_tag="ACZPAQ_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR0903527.1"
                     /translation="MNWFSKDIGIDLGTASVLVYVKGKGVVLREPSVVAMDKNTGKLL
                     KVGAEANAMLGRTPGNIIAMRPLREGVISDYDMTERMLKEFIKKVTSFRLFKPRVVIC
                     VPSGITEVEERAVIDAGIQAGARRVYLIEEPVAAAIGAGIDIAKPDGHMVVDIGGGTA
                     DIAVISLSGVVESASIKIAGDKFDEAVIKYIRRKHNVLIGERTAEELKMTIGCVFPRP
                     EEVTMDIKGRCLMTGLPRVFTVSSSEMIEAFEEPSTRILEAIHGVLERTPPELVADIS
                     TNGIVMTGGGSLIWGFDKLVESHTGIETHVADDAISCVAYGTGKSLDDLDHMQDGTMN
                     LSRRKQMNY"
     gene            complement(2081..2758)
                     /locus_tag="ACZPAQ_05770"
     CDS             complement(2081..2758)
                     /locus_tag="ACZPAQ_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR0903528.1"
                     /translation="MGSALLDLLFPPRCPFCRRLLTERHALLCPDCQRTLPWLEGDSA
                     RRTGEFFGVCVSPLAYRGPVREAVHRYKFSGSRSYARPFGQLMAQCVRDQLDGRFDCV
                     TWAPLSARRLRQRGYDQARLLAEEAARVLEVPALPLLSKTRDTPPQSGLEEAGQRRAN
                     VLGVYRAPDAAAGLRVLLADDVVTTGSTLSECARTLLTAGAAEVVCVTLAQARKDRPE
                     KPRKTAK"
BASE COUNT          579 a          897 c          956 g          555 t
ORIGIN      
        1 tcggaccctg cgtggtttgt accattcctg ctccctgtcc gtgatattgc tttaaatttt
       61 ctttccgtcc agctacatgg accattctct acctgtaccg atatataatg cataacagag
      121 tcctgtctgt gtacaagtat gtactcgaac aggcacaccg gaaaagtctt ttttgtacat
      181 taccttagtt tgaaaagtat ggtaaattgg atgcagaaca tgtcgaagcg ggtgatttta
      241 tggatgattt ttggatcgag cgggccccgg agaaactttc cagcatcagc cggaccatcc
      301 gcttggatgc ggcgctcttt gagcgcatca tggaggagag cgaaaaaaca ggggtctcat
      361 tcaataagat agtcaaccag tgcattacat atgcgttcca gcactaccgg gaaccgaagg
      421 taacaccgga tggaagataa gacgatcctg tattctgtgc gctatccttc ggcccaggag
      481 gagggggagc gcatcaaggt ccggggggac aggcaggcgg cagaggcgaa gctgaccgaa
      541 tttctgcgcc gcatgaggga ggagcggact tatgagccca tcccggggcg ggaggagaag
      601 ggcaggcgct tcgccgccct ggcccgggag ctgtcccaga tctatgaggt gggcctggac
      661 atcctgcgga cgcccgactc catcgaggcc gccctgcacc tgtactgttc cgcctattcc
      721 agggatatga cgcggcggct ggcagagctg ttggggatgt gtgatgtctg tgccgccagc
      781 cggcccagaa cggagcccag tgatcttacc ctctccctca tgctgtacac ccaccgctgt
      841 tatgtgtccg gccggctgat cagcggctga ccggcccaag ggataaaaag gcgcgccgcc
      901 cggctgggcg gctcgcctgt ctgcgtccat caatagttca tctgcttgcg gcgggacagg
      961 ttcatggtcc cgtcctgcat gtggtccagg tcatccaggg acttgcccgt gccgtaggcc
     1021 acacaggaga tggcgtcgtc ggccacatgg gtctcgatgc cggtgtggga ctccaccagc
     1081 ttgtcgaagc cccagatcag ggagccgccg ccagtcatca cgatgccgtt ggtggagatg
     1141 tcggccacca gctcgggggg cgtgcgctcc agcacgccgt ggatggcctc caggatgcgg
     1201 gtggagggct cctcaaaggc ctcgatcatc tcgctggagg agacggtgaa cacccggggc
     1261 aggccggtca tcagacagcg gcccttgatg tccatggtga cctcctccgg gcgggggaag
     1321 acacagccga tggtcatctt cagctcctca gcagtacgct caccgatgag cacgttgtgc
     1381 ttgcggcgta tgtacttgat gacagcctcg tcgaacttgt cgccggcgat cttgatggag
     1441 gcggactcca ccacgccgga cagggagatg accgcgatgt cggcggtgcc gccgccgatg
     1501 tccaccacca tgtggccgtc gggcttggcg atatcgatgc ccgcgccgat ggcggcggcc
     1561 acaggctcct caatcaggta gacccggcgg gcgcccgcct ggatgcccgc atcaatgacg
     1621 gcgcgctcct ccacctccgt gatgccggag gggacgcaga tgaccacacg gggcttgaac
     1681 aggcggaagg aggtgacctt cttgatgaac tccttcaaca tccgctccgt catatcatag
     1741 tcggagatga cgccctcccg cagggggcgc atggcgatga tgttgccggg ggtgcggccc
     1801 agcatggcgt tggcctcggc ccccaccttc agcagcttgc cggtgttctt gtccatggcc
     1861 accacagagg gctcgcggag aaccacgcct tttcccttga catataccag aacggaggcg
     1921 gtacccaggt cgataccgat gtccttgcta aaccaattca tatcattacc tcattcgatt
     1981 tatcttgtat tccaggagag tataaccctg aaactggaaa atatgcaaaa acaggagctt
     2041 tcttatttat tataacggca agcaggctgc atttcaactg ttatttcgca gtttttcgcg
     2101 gcttttccgg cctgtccttc cgggcttgtg ccagggtgac gcacaccacc tccgccgctc
     2161 cggcggtgag gagggtcctg gcacattcgg acagggtgga gccggtggtg accacgtcgt
     2221 cggccagcag gacccggagg ccggcggcgg catcaggcgc tctgtacacc cccagcacgt
     2281 tggcccggcg ctggcccgcc tcctccagac cggactgggg cggggtatcc cgggtcttgc
     2341 tcagcagggg gagggcgggg acctccagca cccgggcggc ctcctccgcc agcaggcggg
     2401 cctggtcata gccccgctgc cgcaggcgcc tggcgctgag aggggcccag gtcacgcagt
     2461 cgaaccgtcc gtccagctgg tcccggacgc actgggccat gagctggccg aaggggcggg
     2521 catagctccg gctgccggag aatttgtagc ggtggacggc ctcccgcacc gggccccggt
     2581 aggccagggg agagacgcac accccgaaaa attcccccgt gcgccgggcg gagtccccct
     2641 ccagccaagg cagcgtccgc tgacaatccg ggcagagcag ggcgtgccgc tccgtcagga
     2701 gccggcggca gaagggacag cggggcggga acaggaggtc gaggagcgcg ctgcccatct
     2761 tcacggaaca tcagcccttt ccggtccggc ctgccgcggg ggcacggcag ccggggatca
     2821 tgccctcatg ataatacgca tcggaaaaag acgcaagtga aagcggagaa aaatgacaga
     2881 gaccggggaa aaagcaaggg tgcccgcccc gtgggcgggc acccttgctt cttaaaaaca
     2941 ccctccccgg aggagagcgg aggtcaggtg gactggacag aggggtc
//
LOCUS       DBMDSJ010000204         2979 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8128_length_2979_cov_1.868311, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000204 DBMDSJ010000000
VERSION     DBMDSJ010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8128_length_2979_cov_1.868311"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            114..1904
                     /locus_tag="ACZPAQ_05775"
     CDS             114..1904
                     /locus_tag="ACZPAQ_05775"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903529.1"
                     /translation="MKQKKPSSLARILGYAGGHKNLTILGCILSALSAVLGLAPYLCV
                     WLVARSVLSAWPSLDGAGDLGRWGWMAVWTAVGSILLYFAALMSTHIAAFRTARNIRR
                     AAMTHVLKLPLGFFTGNQSGRLRKLIDDNAGLTEDLLAHKLPDLAATAVTPIAAIVVL
                     FLFDWRMGLLCLLTMVLALLSMCMMMGGKNAGFFHRYQQEIERMSGEAVEYVRGIPVV
                     KMFQQTVYSFNAFYAAIQDYSDLASQYAMSCRTGQTCFLTFINGAFALLIPAVLLIAS
                     GGDVRAALVNLIFYALFAPACGQMINRIMYMSEAVMEADEAIGRLDEILSQQPMEEPK
                     VQKSFASDAVAFDHVTFTYPGADRPALKDVSFSVQPGEAVALVGPSGGGKTTAASLIP
                     RFWDVDSGSVTVGGADVRELDSTALMGQVAFVFQDTRLFKESLLENIRAARPDASRDE
                     VLAAAHAAQCDDILEKLPQGLDTVVGARGVYLSGGEQQRIALARAILKDAPIVVLDEA
                     TAFADPENEHQIQKAFETLIRNKTVLMIAHRLSTVQNADNIIVLSEGKIAEQGSHSAL
                     LEKSGVYAAMWADYQRSAQWKVKVGKEAAV"
     gene            1901..>2979
                     /locus_tag="ACZPAQ_05780"
     CDS             1901..>2979
                     /locus_tag="ACZPAQ_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012869.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR0903530.1"
                     /translation="MTKKLQHIFALSEKGARDLVKAVIWCFVCNLALMFPVGAVLFTV
                     QHLLGCLESSGSPMDGFWLYVGFALAVLLLLFVLHWFQYASLYIATYQESANRRVSLA
                     ETLRRLPLSFFGNRDLSDLTSTMIADCSSLDQMFSHYVPQLFASIGSTLVIGACMFVC
                     DWRMALAVLWVVPVAVALTAGSKKIQDAFGTKNILNKRAVADCIQEGLETIRDIKACH
                     RQERYLDGLEAKLSAMEGSSIRSELATGVFVCSAQAFLRLGLATTVLTGGALLLVGEL
                     SFLYFLGFLFAAARLYDPLGVVLQNIAATFNTKLQIERMRSILEQPVQTGTEDFCPSS
                     YDITFDHVSFAYREGAGVLEDVSFTA"
BASE COUNT          517 a          918 c          945 g          599 t
ORIGIN      
        1 gctgaagctg atcggccagt cataaggcgc accactttcc agtaagatcg gggcttgccc
       61 ctttcttttt acactatcag ttagtaattg ctaacctaaa gggaggaata tttgtgaagc
      121 aaaagaaacc aagcagcctt gcccgcatcc tgggctatgc gggcgggcac aagaacctaa
      181 cgatcctggg ctgtatcctg tccgccctgt cggcggtgct ggggctggcg ccgtatcttt
      241 gtgtctggct ggtggccaga agcgtgctgt cagcctggcc cagtctggac ggggccgggg
      301 atctgggccg ctggggctgg atggcggtgt ggactgccgt tggcagcatc ctgctttact
      361 ttgccgccct catgtccacc catatcgcgg ccttccgcac cgcccggaac atccggcgcg
      421 ccgccatgac ccatgtacta aagctcccct tgggcttttt caccgggaac cagtcggggc
      481 ggctgcgcaa gctcatcgac gacaacgccg ggctcaccga ggacctgctg gcccacaagc
      541 tccccgacct ggccgctacg gcggtgacgc ccatcgccgc catcgtggtg ctgttccttt
      601 ttgactggcg aatgggcctt ttgtgcctgc tcaccatggt gctggccctg ctgtccatgt
      661 gcatgatgat gggcggcaaa aacgccggct tcttccaccg ctatcagcag gagatcgagc
      721 gcatgagcgg cgaggcagtg gagtatgtcc ggggcatccc ggtggtgaag atgttccagc
      781 agacggtcta ctccttcaat gccttctacg ccgccatcca ggattacagc gacttggcca
      841 gtcagtacgc catgagctgc cggacgggcc agacctgttt ccttaccttc attaacgggg
      901 ccttcgccct gctgatcccg gcggtgctgc tcatcgcctc cggcggggat gtgcgggccg
      961 cgctggtgaa cctgatcttc tacgccctgt tcgcccctgc ctgcggccag atgatcaacc
     1021 gcatcatgta tatgagcgag gcggtgatgg aggccgacga ggccattggc cgcctggacg
     1081 agatcctgag ccagcagccc atggaggaac cgaaggtcca aaagagtttt gccagcgacg
     1141 ccgtggcctt tgaccatgtg accttcacct accccggcgc ggaccggccc gcgctgaagg
     1201 atgtgagttt ctccgtccag cccggtgaag cggtagccct ggtgggaccc tccggtgggg
     1261 gcaagaccac cgccgccagc ctgattcccc ggttctggga tgtggacagc ggcagcgtca
     1321 ccgtgggcgg cgcggacgtg cgggagctgg acagcaccgc tctcatgggg caggtggcct
     1381 ttgtgttcca ggatacccgg ctgttcaagg agagcttgct ggagaatatc cgggccgccc
     1441 ggccagacgc ctcccgggat gaggtgctgg ccgccgccca cgctgcccag tgcgatgaca
     1501 tcctggaaaa actgccccag ggactggaca cggtggtggg tgccaggggc gtctatctct
     1561 ctggcgggga gcagcagcgc atcgccctgg cccgggccat tttgaaggac gctcccattg
     1621 tggtgctgga cgaggccacc gcctttgccg acccggagaa cgagcatcag atccaaaagg
     1681 cctttgagac cctgatccgg aataagacgg tgctgatgat cgcccaccgg ctgtccacag
     1741 tgcagaacgc ggacaacatc atcgtcctca gcgagggaaa gatcgcggag cagggcagcc
     1801 acagcgccct gctggagaaa agcggtgtct acgccgccat gtgggcggac tatcagcgca
     1861 gcgcccaatg gaaggttaag gttggaaagg aggcagcagt atgacaaaga aattacagca
     1921 catcttcgct ctcagtgaga agggggccag agacctggtg aaggccgtga tttggtgctt
     1981 tgtgtgcaat ctggccctca tgttccccgt gggggcggtc ctgttcacgg tccagcatct
     2041 tctgggctgc ctggagagca gcggcagtcc catggacggg ttctggctct atgtgggctt
     2101 tgccctggcg gtgctcctcc tgctgttcgt cctccactgg ttccagtacg cctcccttta
     2161 catcgccacc taccaggaga gcgccaaccg ccgggtgagc ctggcggaga cgctgcggag
     2221 actccccctg tccttcttcg gcaaccggga tctcagcgac ctgacctcca ccatgatcgc
     2281 cgactgctcc agcctggatc agatgttctc ccactatgtg ccgcaactgt tcgcctctat
     2341 cggctccacg ctggtaatcg gggcgtgcat gttcgtgtgc gattggcgca tggccttggc
     2401 ggtgctgtgg gtggtgcccg tggcggtggc gctgacagcg ggaagtaaga agatccagga
     2461 cgcctttggg acgaaaaata tcctgaacaa acgggcagtg gccgattgca tccaggaggg
     2521 gttggagacc atccgggaca tcaaggcctg ccaccggcag gagcggtatc tcgacggcct
     2581 ggaggcaaaa ctgtccgcca tggagggcag ctccatccgc tccgagctgg ccaccggcgt
     2641 gtttgtgtgc tccgctcagg ctttcctgcg gctggggctt gccaccacgg tgctcaccgg
     2701 cggggcgttg ctgctggtcg gggagctctc cttcctctat ttcctgggct tcctctttgc
     2761 cgctgcccgg ctgtacgatc ctctgggggt ggtgctccag aacatcgccg ccaccttcaa
     2821 caccaagctg cagattgaac ggatgcgctc cattctggag cagccggtgc agacggggac
     2881 agaggacttc tgcccatcca gttacgacat caccttcgac cacgtctcct ttgcctaccg
     2941 ggagggcgcg ggggtgctgg aggatgtgag cttcaccgc
//
LOCUS       DBMDSJ010000205         2972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8154_length_2972_cov_1.653779, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000205 DBMDSJ010000000
VERSION     DBMDSJ010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_2972_cov_1.653779"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="ACZPAQ_05785"
     CDS             complement(<1..455)
                     /locus_tag="ACZPAQ_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0903531.1"
                     /translation="MEPVKIIGAGLAGCEAAWQLAQRGIPVELHEMKPEKMTPAHHSP
                     EFAELVCSNSLRSDQLENAVGLLKEELRRCGSLIMSCADAHRVEAGGALAVDRRAFSQ
                     AVTQAIRYHPAVTVVEGEVERIPEEGQVIVAAGPLASDALTEEISRLFP"
     gene            complement(917..1123)
                     /locus_tag="ACZPAQ_05790"
     CDS             complement(917..1123)
                     /locus_tag="ACZPAQ_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903532.1"
                     /translation="MYGERLKFLRKERKIPQKDLAELLGISIRGYQFYESENNEPNIA
                     ALVTLADFYGVTIDFLVGRTDEEA"
     gene            1202..1468
                     /locus_tag="ACZPAQ_05795"
     CDS             1202..1468
                     /locus_tag="ACZPAQ_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903533.1"
                     /translation="MTDLMEALFEYINKVTLQNYLAVDSDYRISSTRRDTWSLEILDH
                     LDEEGRTLFDRYEKAAFQENSCLLEAMFLATVDLCRELNGRLTA"
     gene            complement(1487..>2972)
                     /locus_tag="ACZPAQ_05800"
     CDS             complement(1487..>2972)
                     /locus_tag="ACZPAQ_05800"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR0903534.1"
                     /translation="TVGLITYMRTDSLRLSEEAIAAAKGFIESRYGKEYYPAQARRYK
                     TKGNAQDAHEAIRPSDVTLTPEDVKKDLTSEQYRLYRLIWGRFLACQMASAVYDSVSI
                     EAQSAGYTFRASHSELKFSGFTAVYEEGRDEDDEAPQSPLPDLREGEPLDRKGLKQDQ
                     HFTQPPTRYTEATLIKALEEKGIGRPSTYAPTISTILNREYVVKEGKYLRTTPLGEVV
                     TGLMKDKFNDIVDYAFTAQMEERLDKVEEGEANWKKVLSDFYGGFEAELHQAEQDLDG
                     ERIKVPDEVSEEICPQCGRNLVIKSGRFGRFLACPGWPECDFTMPLVVEMPGKCPKCG
                     GRLFKRTGKSKKTNKQYTYYCCEHLNSKDESKRCDFMTWDVPVKDNCPVCGWTMFKKA
                     GRGAKKPFCINEACSNFTPEEKRGGWRKKTEESGEEAPAQEAAEEGKTAEKKAPAAKK
                     TTAKAAAKKPAAKAAASKKASAAKKPTAKKTAGKKTAAKKTEEG"
BASE COUNT          449 a          978 c          910 g          635 t
ORIGIN      
        1 tccgggaaca ggcgggagat ctcctccgtc agggcgtcgg aggccagcgg gccggcggcg
       61 acgatgacct gcccctcctc cggaatgcgc tctacctccc cctccaccac cgtgacggcg
      121 gggtggtagc ggatggcctg ggtcactgcc tgtgagaagg cgcggcggtc caccgccagg
      181 gcgccccccg cctccacccg gtgggcatcc gcacaggaca tgatgagaga gccgcagcgg
      241 cgcagctcct ccttcagcag tcccaccgcg ttctccagct ggtcggagcg cagggagttg
      301 gagcacacca gctccgcaaa ctccgggctg tggtgggcgg gggtcatctt ctccggcttc
      361 atctcgtgca gctccaccgg gatgccccgc tgggccagct gccaggccgc ctcgcagccc
      421 gccagaccgg cgccgatgat cttcactggt tccatagctc tcctcctcga gatccgcccg
      481 ccctgccgga gggcggcgat gaagtgtttt tgggggttcc gccctgcggc cagaggcggg
      541 cggaggaccg ccccctcatc cgtcgcggca tacgccgcga caccttcccc ccagaagggg
      601 gaaggctttt cgggcggctg aaagccgccc ctgcgggttc ccttcggcct ttgcgtaggg
      661 gcgcgctttg cgcgcccgcc gtccccatga gcggtcctcg tctctgtgca gggacgggtc
      721 ctagacctgc ccgccctata gatgcgggac gtgggaagga agccccctca tccgtcgcgg
      781 catacgccgc gacaccttcc ccccaggagg ggggaaggct tttgggggga tgcggcatcc
      841 gctgggcacc tctttcccga ggggaaaggc tttgggcagc ggtgtgccgg gccgctgcgc
      901 cctatacgca gtaccgttat gcttcctcgt ccgtgcgccc aaccagaaag tcgatggtca
      961 ccccgtaaaa atccgccaat gtcaccaatg cggcaatatt gggctcattg ttctcgctct
     1021 cgtagaactg atatccacgg atgctgatcc ccagcagctc cgcaaggtct ttctgcggaa
     1081 tcttccgttc ttttctcaaa aattttaatc gttctccata catctcttta cttcctcttg
     1141 acacgaataa tctatacgtg ttataacatg tataaatcat tcgtgtttgg agttgatcac
     1201 aatgaccgat cttatggaag ctctctttga gtacatcaac aaagttaccc ttcaaaatta
     1261 cctggctgtg gactccgatt accggatcag cagcacccgg cgggatacct ggtccctgga
     1321 gatcctcgat catctggatg aagaggggcg caccctgttc gaccgctatg aaaaggccgc
     1381 ctttcaggag aactcctgcc tgctggaggc catgttcctg gccaccgtgg acctgtgccg
     1441 ggagctgaac ggaaggctga ccgcctgagc gttcagcccc cgccggtcat ccctcctccg
     1501 tcttcttggc ggcggtcttt ttcccggcgg tcttcttagc cgtgggcttt ttcgccgcgg
     1561 atgccttttt cgaggccgcg gctttggcag caggcttctt ggccgccgcc ttcgcggtgg
     1621 tctttttggc ggccggagcc ttcttctcgg cggtcttgcc ctcctccgcc gcctcctggg
     1681 caggggcctc ctccccgctc tcctccgtct ttttccgcca gccgccccgc ttctcctccg
     1741 gggtgaagtt ggagcatgcc tcgttgatgc agaagggctt tttcgccccc cggcccgcct
     1801 tcttgaacat ggtccagccg cagacgggac agttgtcctt caccgggacg tcccaggtca
     1861 tgaagtcgca tcgcttggac tcgtccttgc tgttgaggtg ctcacagcag taataggtgt
     1921 actgcttgtt ggtctttttg ctcttgcccg tgcgcttgaa cagacgccct ccgcacttgg
     1981 ggcacttgcc cggcatctcc accaccagcg gcatggtgaa gtcgcactcc ggccagccgg
     2041 gacaggccag gaagcggccg aaccggccgg acttgatgac caggttccgc ccgcactggg
     2101 gacagatctc ctccgaaacc tcgtctggca ccttgatgcg ctccccgtcc aggtcctgct
     2161 ccgcctggtg gagctctgcc tcaaagccgc cgtagaagtc agagagcacc ttcttccagt
     2221 tggcctctcc ctcctccacc ttgtccagcc gctcctccat ctgggcggtg aaggcgtagt
     2281 ccacgatatc gttgaatttg tccttcatca ggccggtgac cacctcgccc agaggggtgg
     2341 tgcggaggta cttcccctcc ttcaccacgt actcccggtt caggatggtg gagatggtgg
     2401 gggcgtaggt ggaggggcgt ccgataccct tctcttccag agccttgatg agggtggcct
     2461 cggtgtaccg ggtggggggc tgggtgaagt gctggtcctg cttcagcccc ttccggtcca
     2521 gcggctcccc ctcccgcagg tcggggaggg gggactgggg tgcctcgtcg tcctcgtccc
     2581 ggccctcctc atacacggcg gtaaagccgg agaatttcag ctccgagtgg ctggcccgga
     2641 aggtgtagcc ggcggactgg gcctcgatgg atacgctgtc atagaccgcg cttgccatct
     2701 ggcaggccag gaaccggccc cagatcagcc ggtagaggcg gtactgctcc gaggtcaggt
     2761 ccttcttcac gtcctccggg gtgagggtca cgtcggaggg acggatggcc tcgtgggcgt
     2821 cctgggcatt gcccttggtc ttgtagcgtc gggcctgggc gggatagtac tccttgccgt
     2881 agcggctctc gataaagccc ttggcggcgg cgatggcctc ctcggacagg cgcagagagt
     2941 cagtacgcat ataggtgatc aggcccacgg tg
//
LOCUS       DBMDSJ010000206         2958 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8204_length_2958_cov_1.379769, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000206 DBMDSJ010000000
VERSION     DBMDSJ010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_2958_cov_1.379769"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="ACZPAQ_05805"
     CDS             complement(<1..1039)
                     /locus_tag="ACZPAQ_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903535.1"
                     /translation="MGGLGRKGWRIGPWQRRLLIGLAVALTSQLYLTVWAEGFRVSTA
                     AILYPALLVTMMRESHRPDTGLVTGLCVVIMRLALDLAAGMPPGEALRMEYPGGVFYL
                     CYDCVLCLLIRDRRTASLGRMGAAFLVSDFISNVINLYLSSGVRGLETEARALLPLIG
                     LAVARSFTAAMILWGSRYYHRLLLRQEHERRYQRLFLMTAELKNELYFLKKNSDDIEQ
                     VMTNAYRLYERLGEKEGESGELSALALSIARDVHEVKKDNLRIIRGIEGEVEEVYDQE
                     QMEFQDLMHILENSTRRMLGENRASIRLECRRTADFSTQEHYRLLSILKNLVTNAIEA
                     IQGAGGRGIVLV"
     gene            1242..2462
                     /gene="ilvA"
                     /locus_tag="ACZPAQ_05810"
     CDS             1242..2462
                     /gene="ilvA"
                     /locus_tag="ACZPAQ_05810"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR0903536.1"
                     /translation="MPTLEQFKNARSVLKGVIRETSLIDSPALSKGCGNRVYLKPENM
                     QVTGAYKIRGAYYKISTLTQEQRERGLVTASAGNHAQGVAYAAQAAGVPATIVMPTTT
                     PLVKVNNTKDYGAKVILHGEVFDESAELAAKLAEEEGLTYIHPFNDLEVATGQGTIAY
                     EIFQDLPDVDIILVPIGGGGLAAGVSTLAKLLNPNVKVIGVEPEGASCMKASLEAGHV
                     VSLPTANTIADGTAVKTPGDKVFPYIRDHVDQIITIPDTELVEAFLDVMEKHKMVVEN
                     SGLLSVAALRHLSCQGLNVVCVLSGGNMDVITMASLVQHGLIMRGRIFTFSVLLPDRP
                     GALLSIADILARENGNVIKLEHNQFVNINRQSGVELKVTLEAFGHDHKDRILEALSQA
                     GYQAHEVDTTDFYH"
     gene            2545..>2958
                     /locus_tag="ACZPAQ_05815"
     CDS             2545..>2958
                     /locus_tag="ACZPAQ_05815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR0903537.1"
                     /translation="MDFEALQATRESCRVYSDRPVPRETLAHLVDVARLAPSGCNAQP
                     WRFIIIDEPEARAKLIEALDDDGLTGCPWGDRVPNFILICEDEAHVKPGVAEHYGAQH
                     FAQMDIGMAAMALCYEATSMGLGTCLIGTMNQAKLH"
BASE COUNT          557 a         1030 c          849 g          522 t
ORIGIN      
        1 ccaccagcac gatcccacgg ccgccagctc cctgaatggc ctcgatggcg ttggtcacca
       61 ggttcttcag gatggacagc aggcggtagt gctcctgggt ggagaagtcg gcggtgcgcc
      121 tgcactccag gcggatgctg gcccggttct cccccagcat ccgccgggtg gagttctcca
      181 gaatgtgcat cagatcctgg aattccatct gctcctggtc atagacctcc tccacctccc
      241 cctcgatgcc ccggatgatg cgcaggttgt cctttttcac ctcgtggacg tcccgggcaa
      301 tggacagggc cagggcactc agctcaccgc tctccccttc cttctccccc agccgctcat
      361 agaggcggta tgcgttggtc atcacctgtt caatgtcgtc ggagttcttt tttaaaaagt
      421 acagctcgtt tttcagctcc gccgtcatca ggaacaggcg ctggtagcgc cgctcgtgct
      481 cctgccgcag cagcaggcgg tggtagtacc ggcttcccca caggatcatg gccgcagtga
      541 aggatcgggc caccgccagt ccgatcaggg ggagcagggc ccgggcctcc gtctccagcc
      601 cccgcactcc ggaggacagg tacagattga tcacattgga gataaagtca ctgaccagaa
      661 aggccgctcc catccgcccc agagaggcgg tgcgccggtc ccggatcagc aggcagagca
      721 cacagtcata gcagaggtag aacacgccgc cgggatactc catccgcagc gcctcccccg
      781 gcggcatccc tgccgccagg tccagtgcca gccgcattat caccacgcac agtccggtga
      841 ccagcccagt gtccgggcgg tggctctccc gcatcatggt caccagcagg gccgggtaga
      901 ggatggcggc ggtggagacc cggaagccct ccgcccacac tgtcagatac agctgggagg
      961 tcagggccac tgccagcccg atcagcagcc tgcgctgcca cgggccgatc ctccagcctt
     1021 ttctccccag tccccccatg gcgctctccc cccgtttttt tctattttac tccatccccc
     1081 ggcagctgcg caagcgtccc cctccccctt cccgcaaaat tccacccttt tctaaaaaaa
     1141 tccgctcccg ctcccgcccc ctccattgac tttcctcccg gaattgggta tgattcactt
     1201 tatgtaatac gtcgtccaag cgcggaaagg aagttttgac catgcccaca ctggaacagt
     1261 tcaaaaacgc ccggagcgtc ttaaagggcg tgatccggga gaccagtctc atcgactccc
     1321 cggccctgtc caagggctgc ggcaaccggg tctatctcaa gcccgaaaat atgcaggtca
     1381 ccggcgccta caagatccgg ggcgcttact ataagatcag caccctgacc caggaacagc
     1441 gggaacgggg cctggtcacc gcctccgccg gcaaccacgc ccagggcgtg gcctacgctg
     1501 cccaggccgc cggcgtgccc gccaccatcg tcatgcccac caccaccccc ctggtgaagg
     1561 tgaacaatac caaggactac ggggccaagg tcattctcca cggtgaggtc tttgacgagt
     1621 ccgccgagct ggccgccaag ctggcggagg aggaggggct gacctatatc caccccttca
     1681 acgacctgga ggtggccacc ggccagggca ccatcgccta tgagatcttc caggacctgc
     1741 ccgacgtgga catcatcctg gtgcccatcg gcggcggcgg gctggcggcg ggtgtgtcca
     1801 ccctggccaa gctgctcaac cccaacgtaa aagttatcgg cgtggagccg gagggggcgt
     1861 cctgcatgaa ggccagcctg gaggcgggcc atgtggtctc cctgcccacc gccaacacca
     1921 tcgctgacgg caccgccgtc aagacccctg gagacaaggt gttcccctac atccgggacc
     1981 atgtggacca gatcatcacc atcccagata cggagctggt ggaggctttc ctggatgtga
     2041 tggaaaagca taagatggtg gtggagaact ccggcctgct gtccgtggcc gctctgcgcc
     2101 acctgtcctg ccaggggctg aacgtggtct gcgtcctctc cggcggcaac atggacgtga
     2161 tcaccatggc ctccctggtc cagcacggcc tgatcatgcg gggccgcatc ttcaccttct
     2221 ccgtcctcct ccccgaccgt cccggtgccc tgctcagcat cgccgatatc ctggcccggg
     2281 agaacggcaa cgtcatcaag ctggagcaca accagttcgt gaacatcaac cgccagagcg
     2341 gcgtggagct gaaggtcacc ctggaggcct tcggccacga ccacaaggac cgcatcctgg
     2401 aggcgctgag ccaggccggc taccaggccc acgaggtgga caccaccgat ttctatcact
     2461 gatccggccc tgttgactcg cgctcttttt ggggaagccc ccacgtcaga ccgtatccaa
     2521 caaaacagaa aggggatctt tgaaatggat tttgaggctt tgcaggcgac ccgggagagc
     2581 tgccgtgtgt acagcgaccg ccccgtgccc cgggagaccc tggcccacct ggtggacgta
     2641 gcccggctgg cccccagcgg ctgcaacgcc cagccctggc gcttcatcat catcgacgag
     2701 ccagaggccc gggccaagct cattgaagcg ctggacgacg acgggctcac cggctgcccc
     2761 tggggagacc gggtgcccaa cttcatcctg atctgtgagg atgaggcaca cgtcaagccc
     2821 ggcgtcgcgg agcactacgg tgcccagcac ttcgcccaga tggacatcgg catggctgcc
     2881 atggccctgt gctatgaggc cacttccatg gggctgggca cctgcctcat cggcaccatg
     2941 aaccaggcca agctgcac
//
LOCUS       DBMDSJ010000207         2950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8237_length_2950_cov_1.363285, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000207 DBMDSJ010000000
VERSION     DBMDSJ010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8237_length_2950_cov_1.363285"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="ACZPAQ_05820"
     CDS             <1..609
                     /locus_tag="ACZPAQ_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR0903538.1"
                     /translation="AVERWPIRLEGAVCADIGSSTGGFTDCMLQRGAEKVYAVDSGYN
                     QLDWRLRSDPRVVCMERTNARYLTGEQIPEPLDFFSVDVAFISLRLILPPMRPLVREG
                     AQAVCLVKPQFEAGREKVGKKGVVRDPAVHLEVLEHFLDHAREAGFSVKDITFSPITG
                     PEGNIEYLGWLGTEGEGPVWSGDLRELVEESHRSLQGKEGQP"
     gene            606..1478
                     /locus_tag="ACZPAQ_05825"
     CDS             606..1478
                     /locus_tag="ACZPAQ_05825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR0903539.1"
                     /translation="MRVLLSSNPYRDRGLRVALEARRILNNAGAETALCLPFAPRKGD
                     RLDLPRQVKLGSMEEELKRADVLLCFGGDGTILHAARDATLHDVPILGVNMGSVGFMA
                     ELERGELSLLTLLAAGEYRTEERMMLSVRVLRGEKVLSEDLALNDAVISKGSMARVAE
                     MEVRADGVLVTNLMGDGVIVSTPTGSTAYSMSAGGPIVEPTSRDIIVTPVCAHQLSTR
                     SMVLAPERLVTVQLPRGSRKHLYLAVDGGKALRITGTERVEISCARQSTRLIRLTERN
                     FYQIIYQKLGGYEL"
     gene            1475..1927
                     /locus_tag="ACZPAQ_05830"
     CDS             1475..1927
                     /locus_tag="ACZPAQ_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR0903540.1"
                     /translation="MKRARQAEILNVIRMMDVETQEQLLAELKLRGFTTTQATISRDI
                     KQLRLVKELAPSGSYRYVAADRQEGKSSAGRLRNIFKEGVVSVEAAQNLVVVKTMPGL
                     ASAACSALDGMEIAGMVGSLAGDDTGMLIMRDSASAEAFCSEAHKLLE"
     gene            1955..>2412
                     /locus_tag="ACZPAQ_05835"
     CDS             1955..>2412
                     /locus_tag="ACZPAQ_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0903541.1"
                     /translation="MLSLLHIENIAVIERADIQFDPGFNALTGETGAGKSIVIDAISA
                     VLGERTSRELIRTGAKSALVNAVFTGVSGLGWLEENGVPAGEELLLQREIQGDGRNIC
                     RVDGRLVTVAQLRELGRQLLNIHGQHDGQQLLDPACHLGYLDRSGHTEGEL"
     assembly_gap    2413..2422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2423..>2950
                     /locus_tag="ACZPAQ_05840"
     CDS             <2423..>2950
                     /locus_tag="ACZPAQ_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR0903542.1"
                     /translation="LASFREAYGALAELRRKIGELEMDEAERSRRVDTLTFQIGELER
                     ADLKPGEDRELDGRRQLLRSAGKLMDAVQTAQYALSGDEDGRGAADLLAEAEGAVQGV
                     ARYSDRLSQLQEKLSQLRCEADDAVELLRDLEEELDFSPEELDELEGRLDTIYRLKKK
                     YGSTVEEMLDYLERSR"
BASE COUNT          500 a          867 c         1112 g          461 t
ORIGIN      
        1 gcggtggagc gctggcccat ccgtctggag ggggcggtgt gtgccgacat cggctcctcc
       61 accggcggct tcaccgactg catgctccag cggggcgcgg agaaggtgta cgccgtagac
      121 tccggctaca accagctgga ctggcggctg cgcagcgacc ccagagtggt gtgcatggag
      181 cggaccaacg cccgctacct caccggggag cagatcccgg agcccctgga cttcttctcc
      241 gtggacgtgg ccttcatctc tctccgactg atcctgcccc ccatgcgccc cctggtgcgg
      301 gagggggccc aggcggtgtg cctggtcaag ccccagttcg aggccggccg ggaaaaggtg
      361 ggcaaaaaag gggtggttcg ggacccggcg gtccatctgg aggtgctgga gcacttcctg
      421 gaccatgccc gggaggcggg attttctgta aaggatatca ccttttcccc cattaccggc
      481 ccggagggca atattgaata cctgggctgg ctggggaccg agggggaggg cccggtctgg
      541 tccggagacc tgagggagct ggtggaggag tcccaccggt ccctccaggg gaaggaggga
      601 cagccgtgag agtgctgctc agttccaacc cctatcggga caggggcctg cgggtggcgc
      661 tggaggcccg caggatcttg aacaacgccg gggcggagac ggccctctgc ctcccctttg
      721 cccccaggaa gggggaccgg ctggatctgc cccggcaggt gaagctgggc agcatggagg
      781 aggagctgaa gcgggcggat gtgctgctct gcttcggcgg agacgggacc atcctccacg
      841 cggcccggga cgccaccctc cacgacgtgc ccatcctggg ggtgaacatg gggagcgtgg
      901 gctttatggc ggagctggag cggggcgagc tgagcctgct caccctgctg gccgccggcg
      961 agtatcggac cgaggagcgg atgatgctct ccgtccgggt gctccggggg gagaaggtgc
     1021 tctcggagga cctggccctg aacgacgcgg tgatctccaa gggctccatg gcccgggtgg
     1081 ccgagatgga ggtccgggcg gacggggtgc tggtcaccaa cctgatgggg gatggggtca
     1141 tcgtctccac ccccaccggt tccaccgcct actccatgtc agcgggcggg cccatcgtgg
     1201 agcccacctc acgggacatc atcgtcactc ctgtgtgcgc ccaccagctg tccacccggt
     1261 ccatggtgct ggcgccggag cggctggtca ccgtccagct cccccggggg agccggaagc
     1321 atctgtacct ggcggtggac gggggcaagg ctctgcgcat caccggaacg gagcgggtgg
     1381 agatctcctg tgcccgtcag tccacccggc tgattcggct gacggaacgg aacttttatc
     1441 agatcatcta tcagaagctg ggagggtatg agctgtgaag cgagcgagac aggcggagat
     1501 tttgaatgtg atccgcatga tggatgtgga gacccaggag cagctgctcg ctgagctgaa
     1561 gctgcggggg ttcaccacca cccaggccac catttcccgg gacatcaagc agctgcggct
     1621 ggtgaaggag ctggccccct ccggcagcta ccgctatgtg gcagcggacc ggcaggaggg
     1681 aaagagctcc gcgggccggc tgcggaacat cttcaaggag ggggtggtct cggtggaggc
     1741 ggcccagaat ctggtggtgg tcaagaccat gcccgggctg gcctcggcgg cctgctccgc
     1801 cctggacggg atggagatcg ccgggatggt gggcagtctg gcgggggacg acaccgggat
     1861 gctcatcatg cgggacagcg cctcggcaga ggcattttgc agcgaggccc acaagctgct
     1921 ggagtgagtt tgagacaggt caggaggggc aaagatgctt tctctgcttc acattgagaa
     1981 tatcgcggtc atcgagcggg ccgatatcca gttcgatcct ggcttcaacg ctctgaccgg
     2041 tgagaccgga gcgggcaagt ccatcgtcat tgacgccatc agtgcggtgc tgggggagcg
     2101 gaccagccgg gagctgatcc gcaccggggc gaagtcggcc ttggtcaacg cggtgttcac
     2161 cggcgtgtcc gggctgggct ggctggagga gaacggggtc ccggcggggg aggagctgct
     2221 cctccagcgg gagatccagg gggacggccg gaatatctgc cgggtggacg ggcggctggt
     2281 gaccgtggcc cagctgcggg agctggggcg tcagctgctg aacatccacg gccagcacga
     2341 cggacagcag ctgctggacc cggcctgcca tctgggctat ctggaccgct ccggacacac
     2401 ggagggggag ctnnnnnnnn nnctggcctc cttccgggag gcatacggag ccttggcgga
     2461 gctgcggaga aagatcgggg aactggagat ggacgaggcg gagcgctccc gccgggtgga
     2521 caccctcacc ttccagatcg gggagctgga gcgggccgac ctgaagccgg gggaggaccg
     2581 ggaactggac gggcggcgcc agcttctgcg cagcgccggg aagctgatgg atgcggtcca
     2641 gaccgcccag tacgccctgt ccggggacga ggacggccgg ggagcggccg acctgctcgc
     2701 agaggccgag ggggctgtcc agggggtggc ccggtacagc gaccgcctct cccagctcca
     2761 ggagaagctg tcccagctgc ggtgtgaggc ggacgacgcg gtggagctgc tgcgggatct
     2821 ggaggaggag ctggacttct ccccggagga actggacgag ctggaggggc ggctggatac
     2881 catctaccgg ctgaaaaaga agtacggctc cacagtagag gagatgctgg attacctgga
     2941 gcggagccgc
//
LOCUS       DBMDSJ010000208         2947 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8245_length_2947_cov_1.815179, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000208 DBMDSJ010000000
VERSION     DBMDSJ010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8245_length_2947_cov_1.815179"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..1552)
                     /gene="spoIVA"
                     /locus_tag="ACZPAQ_05845"
     CDS             complement(74..1552)
                     /gene="spoIVA"
                     /locus_tag="ACZPAQ_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR0903543.1"
                     /translation="MEDRKIYEEIALRTEGDIYIGVVGPVRTGKSTFIKRFMETLVIP
                     NIENVYRRERARDELPQSGSGRTIMTAEPKFVPEEAVSISMDGGAAFQVRLIDCVGYM
                     VDGAVGQLEGEEQRMVTTPWFDREIPMSEAAEIGTRKVIAEHATIGMVITTDGSITDI
                     PREDYLEAEERVIAELKELGKPFLVVLNSAYPNSERARAIRADIAQRHQVACVSANCL
                     ELGEEDISGLLKQVLYEFPLKELDLFLPPWVDALPQEHPIKAALYGAIRQGAGQLHRI
                     RDVDGAIGAMRECEQVSQARVTNIDLGTGLAAAVLELPRALFYDTLSQQSGFQIGDDG
                     DLMALLTQLAQVKAEYDKVADALKEVEETGYGIVVPSIDSLVLEEPEIVRQGGRYGVR
                     LKASAPSIHMIRADIETEVSPIVGSEKQSEEMIHYLLQEFEGDTAKIWQSNIFGKSFH
                     ELVGEDLNAKVKRMPEDAREKLRETLQRIINEGSGGLICIIL"
     gene            complement(1713..>2947)
                     /locus_tag="ACZPAQ_05850"
     CDS             complement(1713..>2947)
                     /locus_tag="ACZPAQ_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR0903544.1"
                     /translation="EGQGRVHACVRLRGAEARALNPRKVLLRADLAVDVTAFQPRERV
                     CCQGVLEPEEHGVQQRVTEQESYQLEAVQEKPFTFSEQVRLNVGQGEPVQLAACRAVP
                     LCGESRLIGSKLIFKGAVELQLLVQEAGGGLNACRENLPFSQIMEVPGLGEVGECRMA
                     VELTQLSCQPAGEDGRTVEVALDLLAQAQVWSRRPVTLLQDLYSTALQTQVEREEQVL
                     WQLVEQSSRTQNVRELLETGSLPRSVTDSWVSLGEIRRNREGDQLVLEGEARVTILYL
                     DENDALQALQRPLTVSCRLDAPEGARCCCRTACSGEVFAAPSAGGIEVRFAVDFQFLI
                     LREQHTTAVCGAALGEPRTRGEGDQPSVVLRLASPGEGLWELAKAYGTTTEQIVQANG
                     LEEGQLPLGRMLLIPSVR"
BASE COUNT          450 a         1088 c          898 g          511 t
ORIGIN      
        1 ctcccctcca ataactcata gaatggcggc aggcagaggt gggctcccac ctccgcctgc
       61 cgcttgcaga ctgtcacaga atgatgcaga tcaggccgcc ggatccctcg ttgatgatcc
      121 gctgaagggt ctccctcagc ttctcccggg cgtcctccgg catcctcttg accttggcgt
      181 tcaggtcctc ccccaccagt tcgtggaagg attttccgaa aatgttggac tgccagatct
      241 tggccgtgtc cccctcaaac tcctggagca gatagtggat catctcctcg ctctgtttct
      301 cgctgcccac gatgggggac acctccgtct cgatgtccgc ccggatcatg tgaatggagg
      361 gtgcgctggc cttcaaacgc acgccgtacc gcccgccctg gcggacgatc tccggctcct
      421 ccagcaccag ggagtcgatg gagggcacca cgatgccgta tccggtctcc tccacctcct
      481 tcagagcgtc ggccaccttg tcgtactccg ccttcacctg ggccagctgg gtgagcaggg
      541 ccatcaggtc cccgtcgtcc ccgatctgga accccgactg ctgggacagc gtatcataga
      601 ataaggcccg gggcagctcc agcacggcgg cggccaggcc ggtgcccagg tcgatgttgg
      661 tcacccgggc ctggctgacc tgctcgcact cccgcatggc cccaatggcc ccgtccacat
      721 cccggatgcg gtggagctgc cccgccccct gccggatggc cccgtacagg gcggccttga
      781 tggggtgctc ctggggcagt gcgtccaccc aggggggcag aaacaggtcc agctccttca
      841 gcgggaactc atacagcacc tgcttgagca ggccgctgat gtcctcctcc cccagctcca
      901 ggcagttggc cgacacacag gccacctggt gccgctgggc gatgtccgcc cggatggccc
      961 gggcccgctc ggagttgggg taggcggagt tgagcaccac caggaagggt tttccaagct
     1021 ccttcagttc ggcgatcacc cgctcctccg cctccagata gtcctcccgg gggatgtccg
     1081 tgatggagcc gtcggtggtg atgaccattc cgatggtggc gtgctcggcg atcaccttcc
     1141 gggtgccgat ctccgccgcc tcggacatgg ggatctcccg gtcgaaccag ggggtggtca
     1201 ccatccgctg ctcctccccc tccagctggc ccaccgcgcc gtccaccata tagcccacgc
     1261 agtcgatgag ccgcacctgg aaggccgcgc cgccgtccat ggagatggac accgcctcct
     1321 ccggcacgaa cttgggttcc gccgtcataa tggtgcggcc cgagccgctc tggggcagct
     1381 catcccgggc ccgctcccgg cggtagacat tttcgatgtt ggggatgacc agagtttcca
     1441 tgaaccgctt gataaaggtg gattttccgg tgcgcaccgg ccccaccacc ccgatgtaga
     1501 tgtcgccctc ggtccgaagc gcgatctcct cgtagatttt gcgatcttcc acttgaattc
     1561 ctccttcggc ccgcgagccg gtcatttccc tacactatat ggggataagc tgtgagatat
     1621 gacggctccc cgccgccatt ccagaccgaa aaccgccccg gccgcacaag cggccggggc
     1681 gggatggcgc gtcaaaacgc cctgtaaggc ggtcagcgca cgctggggat caggagcatc
     1741 cggcccaggg gcagctgtcc ctcctccagg ccgttggcct ggacgatctg ctccgtggtg
     1801 gtgccgtagg ccttggccag ctcccacagg ccctccccgg gggaggccag ccggagcacc
     1861 accgagggct gatccccctc gccccgggtc cggggctccc ccagggcggc cccgcacacc
     1921 gccgtggtgt gctgctcccg gaggatgagg aattggaagt ccaccgcgaa gcgcacctcg
     1981 atccccccgg cggagggggc ggcgaacacc tccccggagc aggccgtccg gcagcagcac
     2041 cgggccccct ccggggcgtc caggcggcag gagacggtca gaggcctctg gagggcctgg
     2101 agggcgtcgt tttcgtccag atagaggatg gtgacccggg cctctccctc cagcaccagc
     2161 tggtccccct cccggtttct ccggatctcg cccagggaca cccagctgtc cgtcacgctg
     2221 cggggcaggg aacctgtctc cagcagctcc cgcacgttct gggtgcggga agactgctcc
     2281 accagctgcc acaggacctg ctcctcccgc tccacctggg tctggagggc ggtgctgtac
     2341 aggtcctgga ggagggtgac cgggcggcgg ctccacacct gggcctgggc cagcaggtcc
     2401 agcgccacct ccacggtgcg gccgtcctcc ccggcgggct ggcagctcag ctgggtcagc
     2461 tccaccgcca tgcggcactc gcccacctcg cccagccccg ggacctccat gatctgggaa
     2521 aaggggaggt tctcccggca ggcgttcagc ccgccgccgg cctcctgcac cagcagctgg
     2581 agctccaccg cccccttgaa gatcagcttg ctgccgatga gacggctctc accgcacagc
     2641 ggtacggcac ggcaggcggc cagctgcacc ggctccccct gccccacgtt cagcctgacc
     2701 tgctccgaaa atgtaaaggg tttttcctgc acagcctcca gctgatagct ctcctgctcc
     2761 gtcacccgct gctgcacccc gtgctcctcc ggttccagca ccccctggca gcacacccgc
     2821 tccctgggct gaaaagcggt cacatccacc gccagatcgg cccggagcag cacctttctt
     2881 ggattcaggg cccgggcctc cgccccccgg agccggacgc aggcgtggac ccggccctgc
     2941 ccttctc
//
LOCUS       DBMDSJ010000209         2938 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8272_length_2938_cov_1.876983, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000209 DBMDSJ010000000
VERSION     DBMDSJ010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_2938_cov_1.876983"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="ACZPAQ_05855"
     CDS             <1..1665
                     /locus_tag="ACZPAQ_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR0903545.1"
                     /translation="TALVRFLFPILALLVLVRAIRGLLRVPHTPEQWGQLSLPGGGSL
                     PIDHWENILGRSSSADIRLNFSTVSRQHAALLRDESGSWWVTDLGSKGGTQVNGVQVF
                     QRTPIRVGDTLTVGGVDLLFLPLSREEGEQLSRRRQEEAPLPMWPSLLWLTLFQLLAA
                     LQLAVSAGASVSPSLFLLFPGLPTVMWTYYLALRRCGARGFEMETIAFFLSTLSLAVT
                     ASSAPGSVLKQFIAILLGLTALVVLGVWLRDTSRTQRLRWLMAAAAIALLSVTLVLGQ
                     TRFGAANWIILGPLSFQPSEVAKIFYIFAGSATLERLFHRRNLGLFMVLTGVCLLCLA
                     LMSDFGTALIFFATFLVIAYLRSGDFATLSLICGGALFAGLLVLNFKPYIFRRFASWG
                     HAWEFASTTGYQQTRAMSAAASGGLVGVGAGEGWLHTVPAADTDLVFGMLCEEWGLLI
                     ALLAVVSIVTLALFAARACRVGRSAFYTIAACAAGSLMVVQTALNIFGAMDLLPLTGV
                     TLPFVSNGGSAMLASWGLLAFLKASDTREKSSFAIRSPAGKEVPVP"
     gene            1662..>2938
                     /locus_tag="ACZPAQ_05860"
     CDS             1662..>2938
                     /locus_tag="ACZPAQ_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR0903546.1"
                     /translation="MKKIEHRAVLCLVLAGLLLAGLALFLFRFFSDGGHWASSPFNRH
                     LYNSAGQLISGTLLDRDGEVLSAVDQSGSRTYPDGETRRKSILHVVGDPYGSIGTGAL
                     TAFADRLTGFDLLNGAFGAQRGNQVYLTIDADLNETAYRALNGRKGTVAVYNYQTGEI
                     LCLVSSPSFDPRNIPSGLETDPSYDGVYLNRFLSSTFPPGSIFKTVTLAAALEELPGL
                     EERTWVCEGSAQIGDGTVTCTAAHGEQDIREALANSCNVVFGQLAVELGGSTLERYAG
                     KAGLTSRYSVNGIPTAAGSFSLTGISDNDLAWAGVGQYHDAVNPCSMLVYMGAIANGG
                     RAAVPCLLLQVDTPGLPDLPQFTRRTGRLIARDTAETLADMMAYNVTAAYGTSRFPNM
                     DLCAKSGTAEVGGGQAPHAWFTGLLRDEDHPYA"
BASE COUNT          377 a         1118 c          882 g          561 t
ORIGIN      
        1 accgccctgg tccgcttcct cttccccatc ctcgccctgc tggtgctggt gcgggccatc
       61 cgcggcctgc tgcgggtccc ccacaccccg gagcagtggg gacagctcag cctgcccggc
      121 ggcggctccc tccccatcga ccactgggaa aatatcctgg gccgctcctc ctccgcagat
      181 atccgcctca atttttccac cgtctcccgc cagcacgccg cccttctccg ggacgagtcc
      241 ggttcctggt gggtcacgga cctgggctct aagggcggca cccaggtcaa cggcgtccag
      301 gtcttccagc gcacccccat ccgggtgggg gacaccctca ccgtgggggg cgtggacctg
      361 ctcttcctcc ccctgtcccg ggaggagggg gagcagctct cccgccggcg gcaggaggag
      421 gcccctctgc ccatgtggcc ctccctgctg tggctgactt tgttccagct gctggctgcc
      481 ctccagctgg ccgtcagtgc gggggcgtcg gtctccccca gcctgtttct cctcttcccc
      541 ggcctcccca ccgtcatgtg gacctactat ctggctttgc gccgctgcgg cgcccggggg
      601 ttcgagatgg agaccatcgc cttctttctc tccaccctct ccctggccgt caccgcctcc
      661 tccgcccctg gctctgtgct gaagcagttc atcgccatcc tgctggggct gaccgccctt
      721 gtggtgctgg gggtgtggct gcgggacacc agccgcaccc agcggctgcg ctggctgatg
      781 gctgcggctg ccatcgccct gctgtccgtc accttggtgc tgggacagac ccgctttggg
      841 gcggccaact ggatcatcct gggccccctc tcctttcagc cctctgaggt ggccaagatc
      901 ttctacatct ttgccggctc cgccactctg gagcggctgt tccacaggcg caacctgggg
      961 ctgttcatgg tgctcaccgg ggtatgtctg ctctgtctgg cgctgatgag cgacttcggc
     1021 accgccctca tcttcttcgc caccttcctg gtgatcgcct acctccgctc cggcgacttc
     1081 gccaccctgt ccctgatctg cggcggggcc ctgtttgccg gcctgctggt cctcaacttc
     1141 aagccctaca tcttccgccg cttcgcctcc tggggccacg cctgggagtt cgcctccacc
     1201 accggctatc agcagacccg ggccatgtcg gccgccgcct ccggcggcct ggtgggggtg
     1261 ggggccggag agggctggct gcacaccgtg ccggcggcgg acaccgacct cgtattcggc
     1321 atgctgtgcg aggagtgggg cctgctcatc gccctgctgg cggtggtctc tatcgtcacc
     1381 ctggccctct ttgccgcccg ggcctgccgg gtgggccgct ccgctttcta taccatcgcc
     1441 gcctgcgccg cgggctcact catggtggtg cagaccgccc tgaacatctt cggggccatg
     1501 gacctgctcc ccctcaccgg cgtcaccctc ccctttgtct ccaacggcgg ctccgccatg
     1561 ctggcctcct ggggcctgct ggccttcctg aaggcctctg acacccggga aaagtccagc
     1621 tttgccatcc gctctcccgc cggaaaggag gtacctgtcc cgtgaaaaaa atcgaacacc
     1681 gggccgtgct ctgcctggtg ctggccggcc tgctgctggc cggcctggcc ctcttccttt
     1741 tccgcttctt ctccgacggg gggcactggg cctcctcccc cttcaaccgc cacctgtaca
     1801 acagcgccgg acagctcatc agcggcaccc tgctggaccg ggacggtgaa gtgctctctg
     1861 ccgtggacca gtcgggcagc cgcacctatc ccgatgggga gacccgccgc aagtccatcc
     1921 ttcacgtggt aggcgacccc tacggcagca tcggcaccgg ggccctcacc gcttttgctg
     1981 accgcctcac cggctttgac ctgctcaacg gcgccttcgg ggcccagcgg ggcaatcagg
     2041 tgtacctcac catcgatgcg gacctgaatg agaccgccta ccgggccctc aacggccgga
     2101 agggcacggt ggccgtctac aactaccaga cgggggagat cctgtgcctg gtgtcctccc
     2161 cctccttcga tccccggaac atcccctccg gcttggagac cgatcccagc tatgatggtg
     2221 tctatctcaa ccgctttctc tcctccacct tcccgcccgg ctccatcttc aagacggtga
     2281 ccctggcggc agcgctggag gagctgcccg gcctggagga gcgcacatgg gtctgtgagg
     2341 gctcggccca gatcggggac gggacggtca cctgcaccgc cgcccacggg gagcaggaca
     2401 tccgagaggc cctggccaac tcctgcaatg tggtgttcgg ccagctggcg gtggagctgg
     2461 gcggctccac cctggagcgc tatgccggga aggcgggcct gaccagccgc tactctgtca
     2521 acggcatccc cactgccgcc ggcagctttt ccctcaccgg aatttcggac aacgacctgg
     2581 cctgggcagg cgtgggccag taccacgacg cggtgaaccc ctgctccatg ctggtctaca
     2641 tgggggccat cgccaatggt ggaagggccg ccgtcccctg cctgctcctt caggtggaca
     2701 cgccgggcct gcccgatctg ccccagttca cccgccgcac cggccgcctc attgcccggg
     2761 acaccgccga gaccctggcc gacatgatgg cctacaacgt caccgccgcc tacgggacca
     2821 gccgctttcc caatatggac ctgtgcgcca agtcggggac agcggaggtg gggggcggcc
     2881 aggcccccca cgcctggttc accggcctcc tccgggatga ggaccacccc tacgcctt
//
LOCUS       DBMDSJ010000210         2937 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8278_length_2937_cov_2.070874, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000210 DBMDSJ010000000
VERSION     DBMDSJ010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8278_length_2937_cov_2.070874"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="ACZPAQ_05865"
     CDS             <1..416
                     /locus_tag="ACZPAQ_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR0903547.1"
                     /translation="ADLQPAPTRAQAERAVYEKYRRGEGLTPAEARLLMPSRAEFVGL
                     WRYLQRRCAGLGRVEDTASRIARGASRATGQREAAERVLLCLEVLAERGLIDLETGPQ
                     GVAVSLRQVERKVDLEASPILGRLRQAIRGGSAEA"
     gene            489..2723
                     /locus_tag="ACZPAQ_05870"
     CDS             489..2723
                     /locus_tag="ACZPAQ_05870"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR0903548.1"
                     /translation="MDPILERYQALEDKVSAYTPGLDTQRLYRAFTYADAEHKGQLRK
                     DGTPYITHPLAVAEIVADLGLDADSVIAALLHDTIEDTNATHEEVAKQFGETVAALVE
                     GVTKLSRVQYTSKEEEQMENLRKMFMAMAQDIRVILIKICDRLHNMRTMEYQSPAKQR
                     EKSLETMEIYAPIAHRLGMQKLKWELEDLSLRYLDPVGYKEIEDELAARSSAHEEFLA
                     AVQQRITQRMWEEGIRCKVYGRVKHTYSIYRKMFAQNKTLDEIFDLYAFRVIVDDIPE
                     CYNVLGCIHDMFKPVLGRFKDYIGTPKPNMYQSLHTTVIGREGVPFEVQIRTWAMHQT
                     AEYGVAAHWKYKQGLANKQLGTEHDFEWVRKLLESQQDTDPDEFVRTLKVDMFADEVF
                     VFTPNGDVKSLPAGATPIDFAYSVHSAVGNRMVGAKVNGRMVPYDHQLSNGDIVEVLT
                     SKTAKGPSRDWLKIAKSNEARNKIRQWFKKEKREENIATGRAAFESELKHVGLKLEAI
                     TAPEVLPSILRKVRFGTLDELYASIGYGGTTAVKAVARIRDEMLRLGRLQAEKAAAAS
                     KTTAESVIVPASSQEVPVLKGSNKHSDSGIIVEGLGNCLVKFAKCCTPVPGDPVVGFI
                     TRGYGVSVHRQDCPNADPGKRKPEEAARWVKVSWVEGSLPSYQTSLELSAKDRDGLTL
                     DVAMALSTAKVKVSALSARSMPDGYASVSVVLEVKDREELSSVINKLNNIQGVYQVVR
                     ASGK"
     gene            2752..>2937
                     /locus_tag="ACZPAQ_05875"
     CDS             2752..>2937
                     /locus_tag="ACZPAQ_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIR0903549.1"
                     /translation="MVWEILGILFAWTWPIWAFVFVFCLAAAISRAVQEAGDGKSRGS
                     GWYAFGAALALAVILGGL"
BASE COUNT          555 a          899 c         1012 g          471 t
ORIGIN      
        1 tggcggacct gcagccggcc cccacccggg ctcaggcgga gcgggctgtc tatgagaaat
       61 accgccgggg ggagggcctg accccggcag aggcccggct gctgatgcct tcccgggcgg
      121 agtttgtggg gctgtggcgc tatcttcagc gccggtgcgc cgggctgggc cgggtggagg
      181 acaccgcctc ccgcattgcc cggggggcct cccgggccac cggacagagg gaggccgccg
      241 agcgggtcct gctgtgcctg gaggtgctgg cggaacgggg gctgatcgac ctggagaccg
      301 gcccgcaggg ggtggcggtc tccctgcggc aggtggagag gaaggtggac ctggaggcct
      361 cccccatcct gggccgtctg cggcaggcca tccgcggcgg cagtgcggag gcgtagcgcc
      421 gaggagagaa ggcgctgcgc gctttaggcg gcccccgctt tactctaaac ttttgcgagg
      481 tgatccgaat ggaccctatt ctggaacgat atcaggcgct ggaagacaag gtgagcgcct
      541 ataccccggg actggacacc cagcgcctgt accgggcgtt tacctatgcg gacgcggagc
      601 acaaggggca gctgcgcaag gacggcaccc cctatatcac ccatcccctg gcggtggcgg
      661 agatcgtggc cgacctgggg ctggacgcgg actcggtgat cgcggccctg ctccatgata
      721 ccattgagga caccaacgcc acccacgaag aagtggccaa gcagttcggc gagacggtgg
      781 ccgccctggt ggaaggggtg accaagctgt cccgggtgca gtacacctcc aaggaagagg
      841 agcagatgga gaacctgcgg aagatgttca tggccatggc ccaggacatc cgggtgatcc
      901 tcatcaagat ctgtgaccgg ctgcacaaca tgcgcaccat ggaataccag tcccccgcca
      961 agcagcggga gaagtctctg gagaccatgg agatctacgc gcccatcgcc caccgcctgg
     1021 gcatgcagaa gctgaagtgg gagctggagg acctgtccct gcgctacctg gacccggtag
     1081 gctataagga gatcgaggac gagctggcgg ccaggtcctc tgcccatgag gagttcctgg
     1141 ccgccgtcca gcagcgcatc actcagcgga tgtgggaaga gggcatccgc tgcaaggtct
     1201 acggccgggt gaagcacacc tattccatct accgcaagat gttcgcccag aacaagacac
     1261 tggatgagat ttttgacctg tatgctttcc gggtcatcgt ggacgacatc ccggagtgct
     1321 acaatgtgct gggctgcatc cacgacatgt tcaagccggt gctgggccga ttcaaggact
     1381 atatcggcac ccccaagccc aacatgtacc agtccctgca caccaccgtc attggccggg
     1441 agggggtccc cttcgaggtg cagatccgca cctgggccat gcaccagacg gcggaatacg
     1501 gcgtggccgc ccactggaag tataagcagg ggctggccaa caagcagctg ggcacggagc
     1561 acgacttcga gtgggtgcgc aagctgctgg agagccagca ggacaccgac ccggacgagt
     1621 tcgtccgcac cctgaaggtg gatatgtttg ccgacgaggt gttcgtcttc actcccaacg
     1681 gggacgtaaa gagcctgccc gccggcgcca cccccatcga cttcgcctac tcggtccact
     1741 ccgcggtggg caaccggatg gtgggggcca aggtcaatgg ccggatggtc ccctatgacc
     1801 accagctctc caacggcgac atcgtggagg tgcttacctc caagacggcc aaggggccca
     1861 gccgggactg gctgaagatc gccaagagta acgaggcccg caacaagatc cgtcagtggt
     1921 tcaaaaaaga gaagcgggag gagaacatcg ccaccggccg ggcagctttt gagtcggagc
     1981 tgaagcacgt gggcctgaag ctggaggcta tcaccgcccc ggaggtgctg cccagcatcc
     2041 tgcgcaaggt gcgcttcggc accttggacg agctgtacgc ctccatcggt tacggcggca
     2101 ccaccgcggt gaaagcggtg gcccgcatcc gggacgagat gctgcgcctg ggccgcctcc
     2161 aggcggagaa ggcggcggcc gcctccaaga ccacggcgga gagcgtcatc gtccccgcca
     2221 gcagccagga ggtcccggtg ctgaaggggt ccaacaagca ctcggactct ggcatcatcg
     2281 tggagggact gggcaactgt ctggtgaagt tcgctaagtg ctgcaccccc gtgcccggcg
     2341 acccggtggt gggcttcatc acccggggct acggggtgtc cgtccaccgg caggactgtc
     2401 ccaacgccga ccccggcaag cgcaagccgg aggaggccgc ccgctgggtc aaggtctcct
     2461 gggtggaggg cagcctgccc agctaccaga ccagtttgga gctgtccgcc aaggaccggg
     2521 acggcctgac cctggatgtg gccatggccc tgtccaccgc caaggtgaag gtgtccgccc
     2581 tgtccgcccg ctctatgccc gacggctacg ccagcgtcag tgtggtgctg gaggtcaagg
     2641 accgggagga gctctcttcc gtcatcaaca agctgaataa catccagggt gtctaccagg
     2701 tggtccgggc cagcggaaaa taatatagat ggacaaaggg ggcaggatgt catggtatgg
     2761 gagatcttag ggatactgtt cgcttggacc tggccgatct gggcctttgt attcgtgttc
     2821 tgcttggcgg ccgccatctc cagggccgtt caggaggcgg gagacgggaa gagccggggg
     2881 agcggctggt atgcttttgg agcggcgctg gccctggcgg tcatcctggg cgggctg
//
LOCUS       DBMDSJ010000211         2929 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8308_length_2929_cov_1.270863, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000211 DBMDSJ010000000
VERSION     DBMDSJ010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8308_length_2929_cov_1.270863"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /gene="rpsP"
                     /locus_tag="ACZPAQ_05880"
     CDS             complement(<1..181)
                     /gene="rpsP"
                     /locus_tag="ACZPAQ_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR0903550.1"
                     /translation="MVKIRLRRMGAKKAPFYRIVVADSRYPRDGRFIEEIGTYNPLTT
                     PAELKVDAERAQAWIK"
     gene            complement(242..490)
                     /locus_tag="ACZPAQ_05885"
     CDS             complement(242..490)
                     /locus_tag="ACZPAQ_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903551.1"
                     /translation="MKKWTFFDQMLASFLVIVISSILSALLHLPIVGRLAFLLVGAGF
                     VLHPVVPVNISIQWRARALWFPRLLGASFLLFGLLAQF"
     gene            complement(520..1899)
                     /gene="ffh"
                     /locus_tag="ACZPAQ_05890"
     CDS             complement(520..1899)
                     /gene="ffh"
                     /locus_tag="ACZPAQ_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR0903552.1"
                     /translation="MAFEGLSEKLSAAFKKLRGKGRLSEADVKEAMREIRLALLEADV
                     SYKVVKQFIAQVTERAVGSDVLEALSPAQTIIKIVNEELTALMGGTSTKLEISSKPPT
                     VVMLVGLQGAGKTTNSAKLAGLMKRQNGKRPLLAACDIYRPAAIKQLEVVGGQLDIPV
                     FQMGQTDPVDIARAAIEHARQHGNDMVFLDTAGRLHVDEELMDELKRIKAAVEPTEIL
                     LVVDAMIGQDAVNAAKAFDDALDIDGVMLTKLDGDARGGAALSIKAVTGKPIKFVGVG
                     EKLDQIEVFHPDRMASRILGMGDMLSLIEKAEQNFDRQKALEFQEKLRKNKFTLTDFY
                     EQMAQLKNMGSLSEIAGMLPGVKASDLEGASMDESLLGRMEAIILSMTPYERENPNVL
                     NSSRKRRIAAGSGTQVVDINRLLKQFEMMQAMTKQFAGGKMPKSMRRLMGKKGGKGML
                     GGKMPGLPF"
     gene            complement(1902..2267)
                     /gene="ylxM"
                     /locus_tag="ACZPAQ_05895"
     CDS             complement(1902..2267)
                     /gene="ylxM"
                     /locus_tag="ACZPAQ_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR0903553.1"
                     /translation="MKSQAYRMALLYDFYGDVLTERQKEFYDLYYNEDLSLSEIAENS
                     GITRQGVRDVIVRAEAILTDLEDKTGLIKRFHAMHKQLQQIAEDAEKILERNGSYDDP
                     DIEAAAREIRELAQVLQKE"
     gene            2452..>2929
                     /locus_tag="ACZPAQ_05900"
     CDS             2452..>2929
                     /locus_tag="ACZPAQ_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903554.1"
                     /translation="MSASNTAHVPMDEPHLSRYGANAARALELEGAVRPGTVAREMRR
                     ALAEVERARQELARWAEESGRTPGAVEWLLDNAYLAAREGRMAERAFRRGRPLRRCRN
                     GQSVLQCAARTALWAVPDLDRRRLTVFLSAFQSVLPLTERELSLLVPALTWALLCQL"
BASE COUNT          508 a          919 c          922 g          580 t
ORIGIN      
        1 tcttgatcca ggcctgagcc cgctcggcgt ccaccttcag ctcggcgggg gtggtcagag
       61 ggttgtaggt gccgatctcc tcgatgaaac ggccgtcacg gggatagcgg gaatccgcca
      121 ccacgatacg gtagaaggga gccttcttcg cgcccatgcg gcgcagtctg attttaacca
      181 tttatattca cctccaaaaa tttcttgcgt atatggaaaa caccgaagtg tttgccagcg
      241 ttcaaaattg ggccagcagc ccgaacagga gaaaggatgc ccccagcagg cgggggaacc
      301 atagggcccg cgctctccac tggatggaga tgtttactgg gaccaccggg tggagaacga
      361 agccggcccc caccagaaga aaggccagac ggcccacaat ggggagatgg agcagggctg
      421 acaggatgga cgagataacg atgaccagga agctggcaag catctgatcg aaaaaggtcc
      481 actttttcat ggtccgtgct cctttccgcc gtcggggagt cagaagggga ggccgggcat
      541 cttgccgccc agcatgccct tgccgccctt tttgcccatc aggcggcgca tggacttggg
      601 catcttgccg ccggcgaact gcttggtcat ggcctgcatc atctcgaact gcttcagcag
      661 ccggttgatg tccaccacct gggtgccgga cccggcggcg atgcgccgct tgcggctgga
      721 gttgagcaca ttgggatttt cccgctcata gggggtcatg gaaaggatga tggcctccat
      781 ccgccccagc agggactcgt ccatggaggc cccctccaga tcggaggcct tgacgccggg
      841 gagcatcccg gcgatctcgg acagggagcc catattcttc agctgagcca tctgctcata
      901 gaaatcggtc agcgtgaatt tgtttttccg cagcttctcc tggaactcca gggccttctg
      961 cctgtcgaag ttctgctccg ccttctcgat gagggagagc atgtcgccca tgcccaggat
     1021 acggctggcc atccggtcag ggtggaagac ctcgatctgg tccagctttt cccccacacc
     1081 cacgaacttg atgggcttgc cggtgactgc cttaatggag agggccgcgc cgccccgggc
     1141 gtctccgtcc agcttggtga gcatgacgcc gtcgatgtcc agtgcgtcgt caaaggcctt
     1201 ggccgcgttc actgcgtcct ggccgatcat ggcgtccacc accagcagga tctccgtggg
     1261 ctccacggcg gccttgatgc gcttgagctc gtccatcagc tcctcgtcca catggagccg
     1321 gccggcggtg tccaggaaca ccatgtcgtt gccgtgctgc ctggcgtgct cgatggcggc
     1381 tctggcgatg tccaccgggt cggtctgacc catctggaac accgggatgt ccagctgtcc
     1441 gcccaccacc tccagctgct tgatggcggc ggggcggtag atatcgcagg cggccagcag
     1501 gggccgcttg ccgttctgac gcttcatcag gccggccagc ttggcgctgt tggtggtctt
     1561 gcccgcaccc tgaaggccca ccagcatcac caccgtgggg ggcttggagg agatctccag
     1621 cttggtgctg gtgccgccca tcagggcggt aagctcctcg ttgacgatct tgatgatcgt
     1681 ctgggcgggg gagagggcct ccagcacgtc ggagcccacg gcccgctcgg tcacctgagc
     1741 gatgaactgc ttgaccacct tgtagctgac gtccgcctcc agcagggcca ggcggatctc
     1801 ccgcatagcc tccttgacgt cggcctcgga gagacggccc ttgccccgca gctttttgaa
     1861 tgcggcggag agcttttcag aaagtccttc aaacgccatg gttactcctt ttgaagcacc
     1921 tgggccagct cccggatctc ccgggccgcc gcctcgatgt ctgggtcgtc atagctgccg
     1981 ttgcgctcca ggatcttttc cgcgtcctca gcgatctgct ggagctgctt gtgcatggcg
     2041 tggaaccgct tgatcaggcc ggtcttatcc tccagatcgg tgaggatggc ctcggcccgg
     2101 acgatcacgt cccggacgcc ctggcgggtg atgccgctgt tctccgcgat ctcggagagt
     2161 gaaagatcct cgttatagta gaggtcgtaa aactccttct gccgctcggt gagcacgtcg
     2221 ccgtaaaaat cgtacagcag ggccatgcga taggcctgac ttttcaaact gttccacctc
     2281 ctgttgagat gtcgtaaagc tcaaaaactt tacattcgga atcataccat aacagagccc
     2341 ccttgtcaag tgaaaaatct gtacacttgg cgggggtata ttcccacggc accggggcaa
     2401 aatccataat gatcgcccaa aaaacggtat gatgattcag gaggagccgc catgtctgct
     2461 tcgaacaccg cccatgttcc catggatgaa ccccatctgt cccgatacgg ggccaacgcc
     2521 gcccgggcac tggagctgga gggagcggtc cgcccgggga ctgtggcccg ggagatgcgg
     2581 cgcgcgctgg ccgaggtgga gcgcgcccgg caggagctgg cccgctgggc cgaggagagc
     2641 gggcgcacgc cgggagcggt ggagtggctg ctggacaacg cctatctggc ggcgcgggag
     2701 gggcggatgg cggagcgggc cttccgcagg ggcaggcccc tgcggcgctg ccggaacggc
     2761 cagtctgtcc tccagtgtgc cgcccggacg gcgctttggg cggtccccga cctggaccgc
     2821 cgccgcctga cggtctttct ctccgcgttc cagtccgtac tgcccctcac cgaacgggag
     2881 ctgtccctgc tggtccccgc cctgacctgg gcgctgctgt gtcagctgc
//
LOCUS       DBMDSJ010000212         2928 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8309_length_2928_cov_1.629643, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000212 DBMDSJ010000000
VERSION     DBMDSJ010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8309_length_2928_cov_1.629643"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="ACZPAQ_05905"
     CDS             complement(<1..306)
                     /locus_tag="ACZPAQ_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR0903555.1"
                     /translation="MKRNWKSLMVSGTVLTVLAGALTLPALASVGQRTAALHYQNIKV
                     TLDGRTLDLTDSRGNEVEPFTIDGTTYLPLASISKALGMDVRWDADTNTVILNTKGTG
                     "
     gene            complement(469..1086)
                     /locus_tag="ACZPAQ_05910"
     CDS             complement(469..1086)
                     /locus_tag="ACZPAQ_05910"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR0903556.1"
                     /translation="MSGAEHLGPYLLEVKEGVFPLGGDSLALGAFATVKRGWRVCDLG
                     TGSGCLLLMLAAREGGLSLTGVEREERAAQCARENLVHSGLEGTVLQADWRQVTLPAG
                     GFDLVISNPPYFQPGHGGDGGGARQADCSLEELCRTAARLLRNRGRFALCHRPERLAD
                     LMCALRGSGLEPKRMRLLAHGPEHPPSCVLMEAVRRGRPGLRIEP"
     gene            complement(1083..1706)
                     /locus_tag="ACZPAQ_05915"
     CDS             complement(1083..1706)
                     /locus_tag="ACZPAQ_05915"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR0903557.1"
                     /translation="MTLEQLEEKLRGREPGLMDASGQYAVLVPLVEREGKLHILYEVR
                     SQDMRRQPGEVCFPGGRIEGDETPEACAIRETEEELGIPTASITVLGRLDFVAHRANF
                     IMYPVLARVETAAAERMALNPAEVDHTFLVPLEHIVHNPPLEYSYALEPRPPADFPYQ
                     LIGIPRGYRWQQGRETVPVYLWQGHAIWGLTGRITRHLAGLLGGEMG"
     gene            complement(1724..2041)
                     /locus_tag="ACZPAQ_05920"
     CDS             complement(1724..2041)
                     /locus_tag="ACZPAQ_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903558.1"
                     /translation="MPQKKGALDRIRDRLQANRNRLTYVLLLLGGLLGLAGWALLPEV
                     VAIHAQQAANIGKNTILLADLALTWGFTAAFWVRPREIIYFVAACLGAFLSLSVLIIN
                     VML"
     gene            complement(2123..>2928)
                     /gene="argS"
                     /locus_tag="ACZPAQ_05925"
     CDS             complement(2123..>2928)
                     /gene="argS"
                     /locus_tag="ACZPAQ_05925"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR0903559.1"
                     /translation="SQEQVDKLDLKDDVLCRANGFYTYFAADIAYHRNKLEVRGFDKA
                     INIWGADHHGHVARLQAALDGLGLDGSHRLVVVLMQLVNLMQDGKPVRMSKRSGKAIA
                     LHDLLDEISVDAARYFFNSRASTSPLDFDLDLAVREDSENPVYYVQYAHARICSLVSR
                     LAEEGAAVPAAAGVDAGVLNAPEELALIKALSQYPEELHLAARDYDPSRINRYLVSLA
                     GDFHRFYNACRIKGEEPGVLSARLKLADSVRAVLANGLGLLGVTAPEKM"
BASE COUNT          476 a         1045 c          881 g          526 t
ORIGIN      
        1 gccggtgccc ttggtgttca ggatcacagt gttggtgtcg gcatcccacc gcacgtccat
       61 tcccagcgcc ttgctgatgg aagccagcgg cagataggtc gtcccgtcga tggtgaaggg
      121 ctccacctcg ttgcccctgc tgtccgtcag atccagggtc ctgccatcca gcgtcacctt
      181 gatgttctga taatgcaggg cggccgtccg ctggcccacg gaggccaggg caggcagagt
      241 cagcgctccc gccaggaccg tcagtacagt tccgctcacc atcaggctct tccagttccg
      301 tttcatttca tgttcctccc aaatgtcatt ttatgttgtg tccggctgta tctcgggctt
      361 atctgtttgc cgttcaacta cataatgcgt gggaggtctc gtttattgca tggggcccaa
      421 aatttttctg aaatttttca gcactccagt tgggagcacc tgcgcccctc acggttcgat
      481 ccgcagaccg ggccgccccc ggcgcaccgc ctccatcaga acacaggagg ggggatgctc
      541 cggcccatgg gccagcagcc gcatccgttt gggctccagg ccgctccccc gcagggcgca
      601 catcaggtcc gccagccgct ccggccggtg gcacaaggcg aaccgccccc tgtttctcag
      661 caggcgggcc gcggtgcggc acagctcctc aagggagcag tcggcctgcc gggccccgcc
      721 cccgtccccg ccgtgtccag gctggaagta ggggggattg gagatgacca ggtcgaaccc
      781 gccagccggc agggtcacct gccgccagtc cgcctggagg accgtcccct ccaggccgct
      841 gtggaccagg ttctcccggg cacactgggc cgcccgctcc tcccgctcca ctcccgtgag
      901 agagagcccc ccctcccggg ccgccagcat caggagcaga cagccgctcc ccgtccccag
      961 gtcgcacacc cgccagcccc gcttcactgt agcgaaggcc cccagggcca ggctgtctcc
     1021 ccccagggga aagaccccct cctttacctc cagaaggtag gggcccaggt gctccgcccc
     1081 gctcatccca tctctccgcc cagcaggccc gccagatgcc gtgtgatccg cccggtcagc
     1141 ccccagatgg cgtggccctg ccacagatag acgggcaccg tctccctccc ctgctgccac
     1201 cgatatcccc gtgggatgcc gatcagctgg taggggaagt ccgccggcgg gcggggctcc
     1261 agggcgtagc tgtactccag cggcggattg tgcacgatgt gctccagcgg caccagaaag
     1321 gtgtgatcta cctccgccgg gttcagggcc atcctctctg ccgcagccgt ctccaccctg
     1381 gccagcaccg gatacatgat gaaattggct cggtgggcca caaaatccag ccgtcccaga
     1441 acggtgatgg atgctgtggg gatgcccagc tcttcctccg tctcccggat ggcgcaggcc
     1501 tccggggtct catctccctc tatccgccct ccgggaaagc agacctcccc gggctggcgg
     1561 cgcatgtcct gtgagcgcac ctcatagagg atatgcagct tcccctcccg ctccaccaga
     1621 gggaccagaa ctgcgtactg tcccgaggcg tccatcagcc ccggctctct gcccctcagt
     1681 ttctcctcca gctgttccag cgtcattccg ttccccacct ctttcagagc atcacattga
     1741 tgatgagcac gctcagagac aggaacgccc ccaaacatgc tgctacaaaa tagatgatct
     1801 ccctagggcg gacccagaag gcggcggtga atccccaggt cagggccagg tccgccagca
     1861 ggatggtgtt tttcccgatg ttggccgcct gctgggcgtg gatggctacc acctccggca
     1921 gcagggccca ccccgccagt cccagcaggc cccccagcag cagcaggacg taggtcagcc
     1981 ggttccggtt ggcctggagt ctgtcccgga tccggtccag ggcccctttt ttctgcggca
     2041 tggtatcacg ttcctctctc atggtttgga aaaagccggc cctcagccct gggggctgag
     2101 ggccggctca acgtccagat cgttacatct tctccggcgc ggtgacgccc agcagaccca
     2161 ggccgttggc caggacggcc cgcacgctgt ccgccagctt cagccgggcg gagagcacgc
     2221 cgggctcctc ccccttgatg cggcaggcgt tgtagaagcg gtggaagtcc ccggccaggg
     2281 acaccagata ccggttaatg cggctggggt cataatcccg agccgccaga tggagctcct
     2341 ccggatactg ggacagagcc ttgataaggg ccagctcctc cggggcgttc agcacaccgg
     2401 cgtccacccc cgcagcggcg gggaccgccg ccccctcctc cgccagccgg gacaccaagg
     2461 agcagatgcg ggcgtgggca tactggacat agtacaccgg gttctcgctg tcctcccgca
     2521 ccgccaggtc caggtcaaag tccagggggc tggtgctggc ccgggagttg aagaagtacc
     2581 gggccgcgtc cactgagatc tcgtccagca ggtcgtggag ggcgatcgcc ttgccggagc
     2641 gcttggacat ccgcaccggc ttgccgtcct gcatcaggtt gaccagttgc atcagcacca
     2701 ccaccagccg gtgggagccg tccaggccca gcccgtccag ggcggcctgg agccgggcca
     2761 catggccgtg gtggtcggcg ccccagatgt tgatggcctt gtcaaacccc cgcacctcca
     2821 gcttgttgcg gtggtaggcg atatcggcgg caaagtaggt atagaagccg ttggcccggc
     2881 agagcacgtc gtccttcagg tccagcttgt ccacctgctc ctggctct
//
LOCUS       DBMDSJ010000213         2910 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8377_length_2910_cov_1.588466, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000213 DBMDSJ010000000
VERSION     DBMDSJ010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8377_length_2910_cov_1.588466"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="ACZPAQ_05930"
     CDS             <1..477
                     /locus_tag="ACZPAQ_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR0903560.1"
                     /translation="AALEFWSDISAGRFVNPGHDIECSWFLMEEAKYEDDPELRAKAM
                     EVFDMAIERGWDKEYGGLLYFVDCMGFPPEAYEHDMKLWWPHNEILIASLMMYRDSRD
                     EKYLDWFYKTVDYGYLRRDGKPTEPACKGCTFKGPFHVPRSLIMADQLIGQILEQN"
     gene            518..1372
                     /locus_tag="ACZPAQ_05935"
     CDS             518..1372
                     /locus_tag="ACZPAQ_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR0903561.1"
                     /translation="MTAHNIFETISSQYFQLTNSEKKVADYVLRHRIDVQYMSISELA
                     EECTVADATISRFCRRLGLTGYNAFKLELAKASMTRDSHRADAAILQESSGFHAMCKK
                     LLGENVAVLEQTIQLLDPRQVSRAVDLLRRSRRVVCMGQGSSMVLSEEAWTLFSTVSP
                     KFLFVPDSHLQMNTLALMDPDKLVLFFSYSGSTRDFQDLLEVARQRQVKVVLVSRFPK
                     SPGGQLADVVLQCGADEGPLQAGSVNARMAQLFVLDVLFQEFCMRAPEETEQSREQIA
                     EAVARKHL"
     gene            complement(1539..>2910)
                     /locus_tag="ACZPAQ_05940"
     CDS             complement(1539..>2910)
                     /locus_tag="ACZPAQ_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HIR0903562.1"
                     /translation="ENGTLIPELQALAPKGNRRMGANPHANGGLLLRDLRTPDFRDYA
                     VDVPQPGAVEAQDMTVMGTYVRDVMELNMESRNFRVFGPDETASNRLSPVFEVTGRRW
                     EDPIDEHLDENLDRDGRVMDSMLSEHLCQGWLEGYLLTGRHGFFNSYEAFIRIVDSMV
                     AQHAKWLKVCNQLPWRQKIASLNYVLSSNVWQQDHNGFTHQDPGFLDHVANKKADVVR
                     LYLPPDANCLLSCFDHCIKSRNYVNVMVASKHPRPQWLTMEQAVKHCTQGIGIWQWAS
                     NDQGCEPDVVMACCGDTPTLETLAAVTILRHYLPDLKIRVVNVVDLMKLQPHTEHPHG
                     LTDEDYDALFTKDKPIIFAFHGYPTLVHELTYRRRNKNLHVRGYKEEGTITTPFDMRV
                     QNDIDRFDLVIDTVKRLPQLGNTASYLIQEMQDKLVEHRQYITTYGQDLPEVRDWKWN
                     DGKGNY"
BASE COUNT          527 a          834 c         1005 g          544 t
ORIGIN      
        1 gctgcactcg agttctggtc cgacatctcc gccggccgct tcgtcaatcc gggccacgac
       61 atcgagtgca gctggttcct gatggaggag gccaagtacg aggacgaccc ggagctgcgg
      121 gcgaaggcca tggaggtctt tgatatggcc atcgagcggg gctgggacaa ggagtacggc
      181 ggcctgctct acttcgtgga ctgcatggga ttcccgccgg aggcctatga gcacgacatg
      241 aagctgtggt ggccccacaa cgagatcctg atcgccagtc tgatgatgta ccgggacagc
      301 agggacgaga agtatctgga ctggttctac aagaccgtgg actacggcta tctccgccgg
      361 gacggcaagc ccaccgagcc cgcctgcaag ggctgcacct tcaaagggcc cttccacgtg
      421 ccccgcagcc tgatcatggc ggatcagctg atcgggcaga tcctggagca gaactgaggg
      481 ccggaggacg gccgggggag aagaggaagg agagagcatg acggcgcata acatatttga
      541 gaccattagc agccagtatt ttcagctgac caactcggaa aagaaggtgg ccgactatgt
      601 gctgaggcac cggatcgatg tccagtacat gtccatctcc gagctggcgg aggagtgcac
      661 cgtggctgac gccaccatct cccgcttctg ccggaggctg gggctgactg gatacaacgc
      721 cttcaagctg gagctggcca aggcatcgat gacccgggac agccaccggg cggacgcggc
      781 catcctccaa gagagcagcg gctttcacgc catgtgcaaa aagctgctgg gagagaatgt
      841 ggcggtgctg gagcagacca tccagctgct ggaccccaga caggtgtccc gggccgtgga
      901 cctgctccgg cgatcccgcc gggtggtgtg catgggacag ggcagctcca tggtgctctc
      961 agaggaggcc tggaccctct tctccaccgt gtcccccaaa ttcctgtttg tgcccgactc
     1021 ccaccttcag atgaacaccc ttgcgctgat ggatccggac aaactggtcc tgttcttctc
     1081 ttactccggc tccacccggg acttccagga cctgctggag gtggcccggc agcggcaggt
     1141 gaaggtggtg ctggtgtccc gtttccccaa atcgccgggg ggccagctgg cggatgtggt
     1201 gctccagtgc ggcgccgacg aggggccgct ccaggccggc tccgtcaatg cccggatggc
     1261 ccagctcttt gtgctggacg tgctgttcca ggagttctgc atgcgggccc ctgaggagac
     1321 agagcagagc agggagcaga tcgctgaggc cgtggcgaga aaacacctgt gaccggggcc
     1381 agattgatca gaattcagaa tagataagag ccgtccccct gagaccagtg ggacggctct
     1441 ttctgtctta taacaaaagg acagggggcg gtatgcgatg ccataccgcc ccctgtccct
     1501 gttccgcatt gcacggatgg actgcgatgg gggagggatc aatagttccc cttgccgtcg
     1561 ttccacttcc agtcccggac ctcgggcagg tcctggccgt aggtggtgat atactggcgg
     1621 tgctccacca gcttatcctg catctcctga atgagatagg aggcagtgtt gcccagctgg
     1681 ggcagacgct tgacggtgtc gatgaccagg tcgaagcggt cgatgtcgtt ctgcacccgc
     1741 atgtcgaagg gagtggtgat ggtgccctcc tccttgtagc cgcggacgtg cagattcttg
     1801 ttgcggcggc ggtaggtcag ctcatgcacc agggtgggat agccgtggaa ggcgaagatg
     1861 atgggcttgt ccttggtgaa gagggcgtca tagtcctcgt cagtgaggcc gtgggggtgc
     1921 tcggtgtggg gctggagctt catcaggtcc accacgttga ccacccggat cttcaggtcg
     1981 ggcaggtagt ggcgcaggat ggtgacagca gccagggtct ccagagtggg ggtgtcgccg
     2041 cagcaggcca tgaccacgtc gggctcacag ccctggtcat tggaagccca ctgccagatg
     2101 ccgatgccct gggtgcagtg cttgacggcc tgctccatgg tcagccactg gggacggggg
     2161 tgcttggagg cgaccatgac gttgacgtag ttgcggctct tgatgcagtg gtcgaagcag
     2221 ctgagcaggc agttggcgtc cgggggcagg tagaggcgca ccacgtccgc cttcttgttg
     2281 gccacgtgat ccaggaagcc ggggtcctgg tgagtgaagc cgttgtggtc ctgctgccac
     2341 acgttggagg agaggacgta gttcagggag gcgatcttct gccgccaggg cagctggttg
     2401 cacaccttca gccacttggc gtgctgggct accatggagt ccacaatgcg gatgaaggcc
     2461 tcgtagctgt tgaagaagcc gtggcggccg gtgagcagat agccctccag ccagccctgg
     2521 cacaggtgct cggagagcat ggagtccatg acccggccgt cccggtccag gttctcgtcc
     2581 aggtgctcgt cgatggggtc ctcccagcgg cggccggtga cctcaaagac gggggagagg
     2641 cggttggagg cggtctcgtc ggggccgaag acccggaagt tccgggactc catattcagc
     2701 tccatcacgt cccggacata ggtgcccata acggtcatgt cctgggcctc caccgcgccg
     2761 ggctggggaa catccaccgc gtagtcccgg aagtcggggg tgcgcaggtc ccggaggagc
     2821 aggccgccgt tggcgtgggg gttggcgccc atgcggcggt tccccttggg ggccagggcc
     2881 tggagctcag ggatcagagt gccgttctca
//
LOCUS       DBMDSJ010000214         2906 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8387_length_2906_cov_1.571480, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000214 DBMDSJ010000000
VERSION     DBMDSJ010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8387_length_2906_cov_1.571480"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="ACZPAQ_05945"
     CDS             complement(<1..298)
                     /locus_tag="ACZPAQ_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR0903563.1"
                     /translation="MSTIAIQLLVQVILILINAFFAATEISVISLNANKLRMLEESGD
                     KLAPRLLKMLEQPAGFLSTIQIGITLAGFLGSAFAADSFSEYLVRWICEDLGFTA"
     gene            complement(587..1648)
                     /gene="pyrB"
                     /locus_tag="ACZPAQ_05950"
     CDS             complement(587..1648)
                     /gene="pyrB"
                     /locus_tag="ACZPAQ_05950"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR0903564.1"
                     /translation="MRHLIDFGDLPRHEWDALYARASQIMDDPSQFVDVCKGRVSCNL
                     FYEPSTRTNFSFQTAMLRLGGTVFGFADPNSSSVAKGETLKDTIKMVSGYADVVVMRT
                     PWEGSAKAASLYSAVPVVNAGDGGHMHPTQTMADLTTITRLRGGVDGLSLGLCGDLKN
                     GRTVHSLIKAMAKFRDIKFFLISPRDLAVPEYMRVFMRENNMWFTEVTGLESVIPQLD
                     VLYMTRIQRERFVDPLEYERNKGIYILTRRKLERAKPSMLVMHPLPRVDEISIDVDDD
                     PRAVYFQQARYGMFARMALLEHLALQPRDDAPPAVEIGTKPVCTNPRCITQTEHYLPP
                     LVKKIGGVDCCGFCDAALC"
     gene            1885..2430
                     /locus_tag="ACZPAQ_05955"
     CDS             1885..2430
                     /locus_tag="ACZPAQ_05955"
                     /inference="COORDINATES: protein motif:HMM:NF018271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0903565.1"
                     /translation="MIWVWNFILYSFFGFLLEVAYARATGGRGDRKSLLVLPLCPVYG
                     VGACLILLLPRTVIQNPFTLFLLGGLAATAAEYGMAILYERGLGVSFWDYTGLPGSIQ
                     GKICLPFSLAWGALALPLIYFVHPAVARLTALIPPGVTWTAAATVAADMAVSGVLMKV
                     TGNRDCLRWYQKRAEKAREGG"
     gene            2669..>2906
                     /locus_tag="ACZPAQ_05960"
     CDS             2669..>2906
                     /locus_tag="ACZPAQ_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903566.1"
                     /translation="MNRAVRGWTTLTAGKVLNLLAAALALQGLGDVIPFQAGCRPLEL
                     SGLILKHLQLKKGDSKYTGPRMLCIRGPVYLTGKR"
BASE COUNT          622 a          785 c          935 g          564 t
ORIGIN      
        1 gggcggtaaa gccaagatcc tcacagatcc agcggaccag atactcggag aagctgtccg
       61 ccgcaaaggc ggagccgagg aaaccggcca gcgtgatgcc gatctggatg gtggagagga
      121 agccggctgg ctgctccagc attttcagca gcctgggagc cagtttgtcc ccgctctctt
      181 ccaacatccg cagcttgttt gcgttgaggg agatcaccga gatctcggta gccgcaaaaa
      241 aggcattgat caggatcagg atgacctgga ccagcagttg tatggcaata gttgacacgg
      301 atcgttcctc ctcaaaattg gacatcagga tgcaaaaaag cacagccggc cctatcctgg
      361 atagggcagc tgtgcttttt tacagtaata aatcgggtcg ggcgcagtcg ctgtctgtgt
      421 ccataaacgc ggataactcc ttcgagccgg tccgaccccg aatgggagca gccggcgatc
      481 ctttcccgct gaggggcggg atgataaaaa ggataaagga aagagaggcc tgcgtcaaga
      541 gaaaagaggc ggaattaacg gcatttttac agggagagat caaatcttag cacagagcgg
      601 cgtcacagaa gccgcagcag tccacgccgc cgatcttttt gaccagaggc gggagatagt
      661 gctcggtctg ggtgatgcag cgggggttgg tacagacggg cttggttccg atctccacag
      721 cgggaggagc gtcatcccgg ggttggaggg ccagatgctc cagaagggcc atccgggcaa
      781 acatcccgta gcgggcctgc tggaaataga cggcccgggg atcgtcatcc acgtcgatgg
      841 agatctcatc cacccggggg aggggatgca tcaccagcat gctgggtttg gcccgttcca
      901 gcttgcgccg ggtgaggata tagattccct tgttgcgctc atactccagg gggtctacaa
      961 accgctcccg ctggatgcgg gtcatgtaga ggacatccag ctgggggatg acggactcca
     1021 gaccggtgac ctcggtgaac cacatattgt tctcacgcat gaagacccgc atatattcgg
     1081 gaacggccag atcccgggga gagatgagga aaaacttgat atcccggaac ttggccatgg
     1141 ccttgatgag ggagtgtaca gtgcgtccgt tcttcaggtc gccgcacagg ccgagagaga
     1201 ggccgtctac gcccccccgc aggcgggtga tggtggtcag atcggccatg gtctgggtgg
     1261 ggtgcatgtg gccgccgtcg ccggcattga ccacggggac ggcggagtag agggaggcgg
     1321 ccttggccga gccctcccaa ggggtgcgca tcaccactac atcggcgtag ccggagacca
     1381 tcttgatggt gtccttcagg gtctctccct tggcaacgga agaggagtta ggatcggcga
     1441 agccgaacac cgtcccgccc aggcgcagca tggcggtctg gaaggagaag ttggtgcggg
     1501 tggagggctc gtaaaacaga ttgcacgaca cccggccctt acacacgtcc acaaattggg
     1561 atgggtcatc catgatctgg ctggcccggg catacagggc gtcccactca tggcggggca
     1621 ggtccccaaa atcgatcaaa tgacgcatcg cgctccactc tcctgttatc cgtattcttc
     1681 gagtaatcct accacaaacc gcccttttcc gcaaggcatc atggaaaagt ctgccccact
     1741 ttttccaagg atggcctcag cttccgggaa aacagaccgc aggcagaaaa aggcccggga
     1801 cctggccgtt aatgacaaaa tttgcccgca tgaaagccac tcattttcgc taccctatgt
     1861 agcagagaaa tggagggctg acctttgatc tgggtttgga actttatcct atacagcttt
     1921 ttcggcttcc tgctggaggt ggcctacgcc cgggcaactg gagggcgggg ggaccgaaaa
     1981 agcctgcttg tgctcccgct gtgcccggtg tacggcgtgg gggcctgcct gatcctgctg
     2041 ctcccgcgga ctgtcatcca gaatcccttt acgctgttcc tgctgggggg cctggcggcc
     2101 acggcggcgg aatatgggat ggcaattttg tacgagcggg gactgggcgt ctccttctgg
     2161 gattataccg gcctgccggg cagcatccag gggaagatat gcctcccctt ttccctggcc
     2221 tggggggcgc tggccctccc actcatctac ttcgtccatc cggcggtggc gcggctgacc
     2281 gccctgatcc cgcctggggt gacctggacg gcggccgcca cggtggcagc ggacatggcg
     2341 gtgtctgggg tgctgatgaa ggtgactggg aaccgggact gtctgcggtg gtatcaaaag
     2401 cgggcggaaa aggcccggga gggtggttga cagccccctg agaaaccggt acactgaagc
     2461 tgaccgcggc aagcgggatc ggagctggaa ggactggagg gccggaaatg gaggcaattt
     2521 actataccgt gagcaatatt aatggagatt atgccgaaat ggtatcggat gatgggcaga
     2581 aacactccct gaccatgttt ctgctcccgg aggggaccac ggtggggagc cggctgaagc
     2641 tggagaactt tcaatggagc ctgtgtggat gaacagagcg gtccgcggat ggaccactct
     2701 gactgcggga aaagtcctca atttgctcgc cgcagccctt gctctgcaag ggctagggga
     2761 tgtgattcca ttccaggcgg gctgccgccc cctcgagctc tccggtctca tcctgaaaca
     2821 tctgcaactg aaaaaagggg attcgaaata tacagggccg cggatgctgt gcatccgcgg
     2881 ccctgtatat ttgacgggaa aaagga
//
LOCUS       DBMDSJ010000215         2902 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8401_length_2902_cov_1.846483, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000215 DBMDSJ010000000
VERSION     DBMDSJ010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8401_length_2902_cov_1.846483"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            75..1430
                     /gene="rpoN"
                     /locus_tag="ACZPAQ_05965"
     CDS             75..1430
                     /gene="rpoN"
                     /locus_tag="ACZPAQ_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR0903567.1"
                     /translation="MELVQRQTQQLTQQTLQSLALLQMSTVELEAYVQELAQENPIID
                     LEPPRSEQEAPRDQELFRRVKWLEDSDRQNRYYQRLDAEELDPLAQVGTDGGLEESLS
                     NFLFRQIQQLDLDESAKQLLCYLSACLDEDGYFRIPLDELSRTSGISQRRLARTLELL
                     RSLEPAGVGASSLSQCLELQLLRIHEEGPALAIVRDCLEELAKKHYHAISARLSISQE
                     EVRSAERLIRELNPRPGSIFALPSEISYIQPDILIEEMDGHFTARLRNSDRPYFQINS
                     YYRSLLTQSDDAQVREYLTLKLRQAENVLWAIGQRETTLLRCAQAIAGRQQDFFRSGP
                     SALRPLRLSDIAQELEIHESTVSRAIRDKYVQCPRGVFPLRHFLSRRAAGESGAEVGS
                     CAAKELLRRLIREEDSTKPLSDQKLCTLLTEQGCPISRRTVAKYREELNIPSASGRKR
                     P"
     gene            complement(1442..2188)
                     /locus_tag="ACZPAQ_05970"
     CDS             complement(1442..2188)
                     /locus_tag="ACZPAQ_05970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR0903568.1"
                     /translation="MKLKDQIAVVTGGSRGIGYAVVRAFLKEGAEVVLCASRSETAER
                     AVSQLKAELPEARVWGIWPSLEDPEQVRTAFDRIGAEHGRIDILVNNAGVSESTPFGA
                     YTGELFDRVMDLNVKGMFHCARGAVPWMERQGRGVILNTSSMVSFYGQPAGVAYPVSK
                     FAVNGFTLSLARELAPKGIRVNAVAPGIIETDMLRAVPREVLAPLEAQIPLGRVGQPG
                     EVAEAFVFLASEQASYITGVVLRVDGLARA"
     gene            complement(2431..>2902)
                     /locus_tag="ACZPAQ_05975"
     CDS             complement(2431..>2902)
                     /locus_tag="ACZPAQ_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903569.1"
                     /translation="ELFAYFARRNLRYHPLAQSPAYGVVRRTSSLYHPETVPPSIRYD
                     EALLMLNVTYFDPIGVHFQPERSPLPPAKGICHGVGRAGNLDMLESMDHLVMISCHIH
                     SKIMARNELAAVPIRPASMDYEYGFLTRADVRLSLEEQALLEKIEGEVQREFEA"
BASE COUNT          511 a         1023 c          812 g          556 t
ORIGIN      
        1 cgtcatgcct tatgcctatc ttgtttcccc ttggcatatt tgctataata gctctgaaaa
       61 aggaggcgtc ccttatggaa ctggtccaac ggcagactca acagctcacc cagcagacgc
      121 tccagagcct cgcgctcctt cagatgagca cagtggaatt ggaggcttat gttcaggagc
      181 tcgcccagga gaaccctatc atcgatctgg agccgccccg ctccgagcag gaagctcccc
      241 gggatcagga gctcttccgc cgtgtgaagt ggctggagga cagcgaccgt caaaatcgct
      301 attaccagcg cttggacgcc gaggaattgg acccgctggc ccaggtgggg acggatggcg
      361 gtctggagga atccctctcc aattttctct tccgccagat ccagcagctc gatctggatg
      421 agtctgccaa gcagcttctg tgctacctgt ccgcctgtct ggacgaggat gggtatttcc
      481 gcatcccact ggacgagctc tcccggactt ctggcatctc ccaaaggcgg ctcgcccgca
      541 ctctggagct actgcgctcc ctggaacccg caggtgtggg ggcttcctcc ctctcccagt
      601 gtctggagct tcagttgctc cgcatccatg aggagggccc cgcccttgcc attgttcggg
      661 actgcctgga ggagctggcc aaaaaacatt atcatgccat ctccgcccgt ctctccatct
      721 ctcaggagga ggtccgctcc gccgaacggc tgatccgaga gctgaatccc cgtcccggtt
      781 ccatcttcgc gctccccagc gagatctcct atattcagcc ggatattttg atcgaagaga
      841 tggacggaca tttcactgcc cgcctgcgga acagtgaccg tccctacttc cagatcaact
      901 cctattaccg cagtctcctg acccagtccg atgacgccca ggtgcgggaa tatcttacct
      961 tgaagctccg ccaggccgaa aacgtgctct gggccattgg tcagcgggag accacgcttc
     1021 tccggtgtgc ccaggctatc gccggccgcc agcaggactt cttccgatcg gggcccagcg
     1081 ccctgcggcc cctgcgcctg tccgacatcg cccaagagct ggagatccat gaatccacgg
     1141 tcagccgcgc cattcgggac aaatatgtcc agtgtccccg gggggtcttt cccctccgtc
     1201 attttctctc ccgccgggcc gccggggaga gcggtgccga ggtcggcagc tgtgccgcca
     1261 aggaactgct ccgccgcctg attcgggagg aggacagcac caagcctctc tccgaccaga
     1321 agctatgcac cctgctgacg gagcagggct gccccatctc ccgccgtacc gtggccaaat
     1381 accgcgagga gctgaacatc cccagtgcct ctgggagaaa gcggccgtag cccggcactg
     1441 ttcaggcccg ggccagtccg tccacccgga ggaccacgcc ggtgatatag gatgcctgct
     1501 ccgaggccag gaatacgaag gcctccgcga cctcccccgg ctgccccaca cgccccaggg
     1561 ggatctgggc ctccagagga gccagcacct cccgcggcac tgcccgcagc atgtccgtct
     1621 cgatgatgcc cggggccacc gcattgaccc gtatcccctt cggcgccagc tcccgggcca
     1681 gggacagggt gaagccgttg accgcgaact tggagaccgg ataggcgacg ccggcgggct
     1741 gtccatagaa gctcaccatg gaggaggtgt tcaagatcac gcctctgccc tgcctctcca
     1801 tccagggcac cgccccacgg gcacagtgga acattccctt tacgttcagg tccatcaccc
     1861 ggtcaaagag ctccccggtg tacgccccaa agggcgtgct ctctgagacc cccgcgttgt
     1921 tgaccaggat gtcgatccgc ccatgctccg ccccgatccg gtcaaaggcc gtccggacct
     1981 gctccgggtc ctccaggctg ggccagatgc cccatacccg ggcctccggc agctccgcct
     2041 tcagctggga taccgcccgc tccgccgtct cgctccggct ggcgcacagc accacttccg
     2101 ccccctcctt caggaaggct ctcaccaccg catatccgat cccccggctc ccgccggtca
     2161 ccaccgcgat ctgatccttc agtttcatgg ccctcttccc ctttcttctg tacgttccaa
     2221 ttccgttttg tgtgcccggg gaaacgcttc ccctgccgtt ccagccccct acgggatgag
     2281 aactcaccaa cggatcggga cttttggtat catgccccct atgcccccct ttcatccctt
     2341 cctagacgca agaggagcgg cgcgcttccc ccacaggaag tgcgccgctc ctctcttcaa
     2401 taatatctga tccgggcacc gatctggccc tcacgcctcg aactcccgct gcacctcccc
     2461 ttcgatcttc tccagcagtg cctgctcttc caggctgagc cggacgtccg cccgggtcag
     2521 gaagccatat tcatagtcca tggacgcggg ccggatgggc accgccgcca gctcattccg
     2581 cgccatgatc ttggagtgga tgtgacagga gatcatcacc aggtggtcca tggactccag
     2641 catatccagg ttgcccgccc ggcccacgcc gtggcagatc cctttggccg ggggcagcgg
     2701 ggagcgctcc ggctgaaaat gcacccctat ggggtcgaaa taggtcacat tcagcatcaa
     2761 aagggcctca tcgtaccgga tggacggcgg gaccgtctcc gggtggtaga ggctgctggt
     2821 ccggcggacc accccgtagg ccgggctctg ggccaggggg tggtagcgca ggttccgccg
     2881 ggcaaagtag gcgaaaagct ct
//
LOCUS       DBMDSJ010000216         2901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8407_length_2901_cov_1.667500, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000216 DBMDSJ010000000
VERSION     DBMDSJ010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8407_length_2901_cov_1.667500"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            285..2150
                     /gene="mnmG"
                     /locus_tag="ACZPAQ_05980"
     CDS             285..2150
                     /gene="mnmG"
                     /locus_tag="ACZPAQ_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR0903570.1"
                     /translation="MTRYDAGEFDVAVIGAGHAGIEAALACARMGLRTVCFTINLDAV
                     GNMPCNPAIGGTGKGHLVRELDALGGEMGRAADRACIQYRLLNRGKGPAVWSLRAQAD
                     RREYQKVMKHTLERQENLWVKQSEVVDILTDGGAVSGVVTYTGAVYRVKAAVVATGTY
                     LGGRTIVGEVVRDSGPDGLAAAVPLTACLTGLGLTIRRFKTGTPPRVNARSVDFSRME
                     RQPGDEEGLAFSFQTECPPENRAVCWLTYTNQRTHDIIRANLDRSPLYDGTIEGVGPR
                     YCPSIETKVVRFPDKERHQLFVEPMGLDTEELYIQGFSSSLPEEVQLEMLHTVPGLER
                     AEMTRCAYAIEYDCVDPTQLEATLESKQVPGLYGAGQFNGSSGYEEAAVQGFVAGVNA
                     ALKLLGRPPLILRRDQGYIGVLIDDLVTKGTNEPYRMMTSRTEYRLLHRQDNADRRLT
                     PIGRGLGLVSREQAEAVQVKYEAVDREAARLERAGTPPSAALDTLLAEKGEPPVRDGA
                     RLADLLRRPRITYEELAPFDPERPPLPREIVQEVEITIKYQGYIDRQLRQVAEMKKLE
                     DRPLPPDLDYEAIGGLRLEARQKLAQIRPRNLGQASRVSGVSPADVAVLMVYLGK"
     gene            2168..>2901
                     /locus_tag="ACZPAQ_05985"
     CDS             2168..>2901
                     /locus_tag="ACZPAQ_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0903571.1"
                     /translation="MKLTQVKGSTWVLEANELIPLYLLDDRRCVLLDSGLAKEREEIE
                     KTLLEAGLEPAGILCSHAHVDHCANNRYFQEKYHLPVALTAPEAGMCSSILNLKCYRL
                     LVSPDAAEQEMSEMVHVPDLILPPVDGPIRLAGAMFRILHTPGHSSGHICAVTPDNVC
                     YTGDALMSQALLDAKLPYGLSIQLAMESRERLQELEDCDFFIMAHKGVCTGREIGPLI
                     DANQELVRRRAGEIRDLITEPMDFSQ"
BASE COUNT          533 a          898 c         1021 g          449 t
ORIGIN      
        1 tgcggaacga tggtaacgtc tatttatgta ctgatcaatg tgtcccatcc attttataaa
       61 cagaaccggg aaaagtcaag gaaaacaagc ggtatgggag gcccagacaa ggggaattct
      121 atatcctcag ccacgccgcg gcagcggacc tggtaggctg aggcggggcg agaccgggcc
      181 ttttccttgc gcgtgggccg attttgtgtt acaatgaccc gaaatgcaca gagcaggcgg
      241 cagaggatca tccacgccgc ctgccgggga gacgagaaag agcgatgaca cgatacgacg
      301 caggagaatt tgatgtggcc gtcatcggcg cgggccacgc tggcattgag gccgccctgg
      361 cctgcgccag aatggggctg cggacggtgt gcttcaccat caacctggat gcggtgggca
      421 acatgccctg caacccggcc atcggcggca ccggtaaggg ccatctggtc cgggagctgg
      481 acgccctggg gggcgagatg ggccgggcgg ccgaccgggc ctgcatccag taccgcctgc
      541 tcaaccgggg caaggggccg gcagtctggt ccctgcgggc ccaggcggac cggcgggaat
      601 atcagaaggt gatgaagcac accctggagc gccaggaaaa cctgtgggtg aagcagtccg
      661 aggtggtgga catcctcaca gacggagggg cggtgtcggg cgtggtcact tacaccgggg
      721 cggtctaccg ggtcaaggcg gctgtggtgg ccaccgggac ctatctgggg ggacgcacca
      781 tcgtggggga ggtggtccgg gactccgggc ctgacggcct ggctgccgcc gtccccctga
      841 cagcgtgcct gacggggctg ggcctgacca tccgccgctt caagacgggc acgccccccc
      901 gggtcaatgc ccgcagtgtg gacttctccc gcatggagcg gcagcctggg gatgaggagg
      961 ggctggcctt ctccttccag acagagtgcc cgccggagaa ccgggcggtc tgctggctga
     1021 cctataccaa ccagcgcacc cacgacatca tccgggccaa tctggaccgc tcccccctgt
     1081 acgatggcac catcgagggg gtagggcccc ggtactgccc ctccatcgag accaaggtgg
     1141 tccgcttccc ggataaggag cgccaccagc tattcgtgga gcccatgggg ctggacacgg
     1201 aggagctgta catccagggg ttctcctcct ctctgcctga ggaggtgcag ctggagatgc
     1261 tgcacaccgt tccggggctg gagcgggcgg agatgacccg gtgcgcctat gccatcgaat
     1321 atgactgcgt ggaccccacc cagctggagg ccaccctgga gagcaagcag gtgccgggac
     1381 tgtacggagc gggacagttc aacggctcct ccggctatga ggaggcggcg gtgcagggct
     1441 ttgtggcggg ggtgaacgcc gccctgaagc tgctgggccg cccgccattg atcctccgcc
     1501 gggaccaggg gtacatcggg gtgctcatcg acgacctggt gactaagggg accaacgagc
     1561 cctaccgcat gatgacctcc cgcaccgagt accggctgct ccaccgccag gacaacgccg
     1621 accgccgcct gactcccatc ggccgcgggc tggggctggt gagccgggag caggcggagg
     1681 cggtgcaggt caagtatgag gcggtggacc gggaagcggc ccggctggag cgggccggga
     1741 cacctccctc cgctgccttg gacaccctgc tggcggagaa aggggagcct ccggtgcggg
     1801 acggggcccg gctggccgac ctgctgcgcc ggcccaggat cacctatgag gagctggctc
     1861 ccttcgaccc ggagcgcccc ccccttccca gggagattgt ccaggaggtg gagatcacca
     1921 tcaagtatca gggttatatc gaccggcagc tgcggcaggt ggcggagatg aagaagctgg
     1981 aggaccggcc tctcccgccg gacctggact atgaggccat tggcggcctg cggctggagg
     2041 cccggcagaa gctggcccag atccggcccc ggaacctggg gcaggccagc cgggtgtccg
     2101 gcgtctcccc cgctgatgtg gcggtgctga tggtctatct gggcaagtga ggacaaagga
     2161 gaagaagatg aagctgacac aagtgaaggg cagcacctgg gtgctggagg ccaacgagct
     2221 gatccccctg tacctgctgg acgaccggcg gtgtgttctc ctggacagcg ggctggccaa
     2281 ggagcgggag gagatcgaga agaccctgct ggaggcgggg ctggagccgg caggcatcct
     2341 gtgctcccac gcccatgtgg accactgcgc caataaccgg tattttcagg agaagtacca
     2401 cctccccgtg gcactcaccg ccccggaggc ggggatgtgc agcagtatcc tgaatttgaa
     2461 gtgctaccgg ctgctggtct cccccgatgc ggcagagcag gagatgagcg agatggtgca
     2521 cgtcccggac ctgatcctcc caccggtgga cgggcccatc cggctggcgg gagccatgtt
     2581 ccgcatcctg cacacaccgg ggcactcctc cggccacatc tgtgctgtca ccccggacaa
     2641 cgtgtgctac accggggacg ccctgatgtc ccaggcgttg ctggacgcca agctgcctta
     2701 cggcctgtcc atccagctgg ccatggagag ccgggagcgg ctgcaggagc tggaggactg
     2761 cgacttcttt atcatggctc acaagggggt gtgcaccgga cgagagatcg gccccctcat
     2821 cgacgccaac caggagctgg tgcgccgccg ggccggagag atccgggacc tgatcacaga
     2881 gcccatggac ttcagccaga t
//
LOCUS       DBMDSJ010000217         2888 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8453_length_2888_cov_1.593828, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000217 DBMDSJ010000000
VERSION     DBMDSJ010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8453_length_2888_cov_1.593828"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="ACZPAQ_05990"
     CDS             complement(<1..199)
                     /locus_tag="ACZPAQ_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR0903572.1"
                     /translation="MNYAEESLRLHEAWGGKIEVTARVPVATRDDLSLAYTPGVAQPC
                     LEIQKDIDKSYELTRRSNLVAV"
     gene            462..1550
                     /locus_tag="ACZPAQ_05995"
     CDS             462..1550
                     /locus_tag="ACZPAQ_05995"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR0903573.1"
                     /translation="MYELITGQTLAEYESFIASHPKGHFAQSSLWGKQKSAWTWRAVA
                     VRGQDGRIRGSIAFLIRRMPVFGVSMLYACRGPVCDLEDRETFAALMEGARALAKEYR
                     GYVIKIDPDVPCRNTQFRQLLESFGFRLMQEGKNFEGIQPKFVFRLNVEGKTEEEMLA
                     SFAQKHRYNIRLAVKKGVQVRVCGQEMVHDFTQIMIETGMRDHFVTRNEAYFSNLLKN
                     LGEHARLYMAFHEGTPIAGTLAIWFGDKVWYLYGASSNRHRNLMPNYLLQWNMIQWAL
                     EKKCRIYDFRGVSGDLSEDNPLYGLYKFKKGFHGEFTEFIGEMDLILSKPLYVMIDKA
                     TKLYQHRNVLKYRRENRDKAPDAAKQDG"
     gene            1566..2702
                     /locus_tag="ACZPAQ_06000"
     CDS             1566..2702
                     /locus_tag="ACZPAQ_06000"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR0903574.1"
                     /translation="MEPKYIVREKDLDQNLQVILEKAKGKIIWGVVKGDGYGLGLAYL
                     AQKLNSCGITHFAVTSLPEARGVRDAGLTENPVLMLRGATDPQEAEELLRLDVICSVG
                     SREDAEVLEAAAERLGVTARAHVEIDTGMGRYGFLPAELPWILPLYRGEYAHIRVTGT
                     YTHFHTAGHPKVTAHQFERFQQVLRFLQFMECDPGMIHCCNSLAFWKYPQYHMDAVRL
                     GSVFLGRVSYAEEAGLSRVGWVEAPIQEVREVQAGSNVGYGSACTVQEDTTLAVVGVG
                     YYHGFAVERGYDVYRPQDCLRNMARYLKYMLTGRKLKVYIADTLCPVLGHVGMLNLVA
                     DVTGLECRPGDPVYIPVNPLDRKGMEIEYRPADQEGKPSSRFRP"
BASE COUNT          571 a          852 c          937 g          528 t
ORIGIN      
        1 ccaccgccac cagattggag cggcgggtca gctcatagct tttgtcgata tccttttgaa
       61 tttccaggca gggctgcgcc acgccggggg tataggccag ggacagatcg tcccgggtgg
      121 ccacggggac tctcgccgtc acttcaattt tcccgcccca tgcttcatgg agccggaggg
      181 attcttctgc atagttcatg tgatgtttcc tccttacggg cccaaggccc gtacaaattt
      241 cattgtacca tgaccgccgc cccaggtcaa gagcgccgcc cggaacgagg cggggagggc
      301 ggggggagaa tttctttcgg gcgggggcgg gagaaactgc ggtttttcgt tgcagaaacg
      361 tagaaactat ggtatgataa ttctgtctat ggccttgcgg ggccgtagcc tggaagcaga
      421 ccgctccgta gacaaaatag gaaagaagtg gagttatcga tatgtatgag ctgatcacag
      481 gtcaaaccct ggccgagtat gaatccttta ttgcctccca tcccaagggc catttcgccc
      541 agtccagcct ctggggcaag caaaaatccg cctggacctg gcgggccgtg gccgtccggg
      601 ggcaggacgg aaggattcgg ggctccatcg ccttcctcat ccggaggatg ccggttttcg
      661 gcgtgagcat gctgtacgcc tgccggggtc ccgtgtgcga tctggaggac cgggagacct
      721 ttgccgctct catggagggg gcccgggccc tggccaagga gtaccggggc tacgtcatca
      781 aaatcgaccc cgacgtgccc tgccggaaca cccaattccg gcagctgctg gagtccttcg
      841 gcttccgcct gatgcaggag gggaagaact ttgagggcat ccagccgaaa tttgtcttcc
      901 gcctgaacgt ggaggggaag accgaggagg agatgctggc ctcctttgcc cagaagcacc
      961 ggtacaacat ccgcctggct gtgaagaaag gggtgcaggt ccgggtctgc ggccaggaga
     1021 tggtccacga tttcacccag attatgatcg agaccggcat gcgggaccac ttcgtcaccc
     1081 ggaacgaggc ctatttctcc aacctgttga aaaacctggg cgagcacgcc cggctgtaca
     1141 tggccttcca cgaggggacc cccattgccg gaaccctggc catttggttc ggagacaagg
     1201 tgtggtacct gtacggcgcc tcgtccaacc ggcaccggaa cctgatgccc aattacctgc
     1261 tccagtggaa catgattcaa tgggccctgg agaagaagtg ccggatttac gacttccggg
     1321 gggtctcggg cgacctgtcg gaggacaacc ccctgtacgg cctgtacaaa tttaaaaagg
     1381 gcttccacgg ggagtttacg gagttcatcg gggagatgga cctgattctc agtaaacccc
     1441 tgtacgtcat gatcgacaaa gccaccaagc tctaccagca ccgcaacgtg ctgaaatacc
     1501 gccgggaaaa ccgggacaaa gcgccggacg ccgccaagca ggacggataa agagaggaaa
     1561 ggcatatgga accgaaatac attgtgcgag aaaaggacct ggatcaaaac ctgcaggtga
     1621 ttctggagaa ggccaagggt aagatcatct ggggcgtcgt caagggtgac ggctacggcc
     1681 tgggcctggc ctacctggcc caaaagctga acagctgcgg catcacccat tttgccgtca
     1741 ccagcctgcc ggaggcccgg ggcgtccggg acgccgggct cacggaaaat cccgttttga
     1801 tgctccgagg cgccacggat ccccaggagg ccgaggagct tctccggctg gacgtcatct
     1861 gctccgtggg cagccgggag gacgcggagg tcctggaggc ggcggcggag cggctggggg
     1921 tgaccgcccg ggcccatgtg gagatcgaca ccggcatggg ccggtacggc ttcctccctg
     1981 cggagctccc ctggatcctg ccgctgtatc ggggggaata cgcccatatc cgggtcaccg
     2041 gcacctacac ccattttcac accgccgggc atcccaaggt caccgcgcac cagtttgagc
     2101 gctttcagca ggtgctgcgt ttcctccagt ttatggagtg cgatcccggc atgatccact
     2161 gctgcaactc cctggccttt tggaagtacc cccagtatca tatggacgcg gtgcgcctgg
     2221 gctcggtgtt tctggggcgg gtgtcctatg ccgaggaggc cggtctgagc cgggtcggct
     2281 gggtggaggc cccgatccag gaggtgcggg aggtgcaggc cggcagcaac gtgggctacg
     2341 gcagcgcctg cacggtgcag gaggacacca ccctggccgt ggtgggcgtg ggctattacc
     2401 acggctttgc cgtggagcgg ggctacgacg tgtaccggcc ccaggactgc ctgcgcaaca
     2461 tggcccggta cctcaaatac atgctcaccg gccgcaaact gaaggtatat atcgcagaca
     2521 ccctgtgtcc cgttttgggc catgtgggga tgctgaacct ggtggccgac gtcacgggcc
     2581 tggagtgccg gcccggcgac ccggtctaca ttccggtcaa tcccctggac cggaagggaa
     2641 tggagatcga gtaccgcccg gcggatcagg agggaaagcc ctcgtcccgc ttccgcccct
     2701 aggccgttgc gcgcaagcgg gacccggaga gagatttgga aggagatcca catgaaaaaa
     2761 acgatgttgt ccctgctatt ggcggccctg ctcctgctca ccggctgcgg gcagctggag
     2821 gccctggccg cccggatggc gcaggagcgg gatgcgcagc tgaacgccct ggccgacctg
     2881 ggcagaaa
//
LOCUS       DBMDSJ010000218         2881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8475_length_2881_cov_1.583094, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000218 DBMDSJ010000000
VERSION     DBMDSJ010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_2881_cov_1.583094"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /gene="mnmA"
                     /locus_tag="ACZPAQ_06005"
     CDS             complement(<1..945)
                     /gene="mnmA"
                     /locus_tag="ACZPAQ_06005"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016324024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR0903575.1"
                     /translation="MGERIAVAMSGGVDSSVAAWLLQREGHELMGVTLRLFDRETPEG
                     TCGASDDLADARAVAERLGMPHQVLDCRTCFRERVIRPFAAAYEAGCTPNPCVDCNRG
                     IKFGELLRRSLELGAEAVATGHYVRMERDGATGRCLLKKALHPEKDQSYVLYSLTQEQ
                     LSRSRFPLGGLSKEEIRAIAARQGFGNAEKKESQDICFIPDGDYAAFLRRYMGREYPP
                     GPFLDQNGRRLGTHEGIVSYTVGQRRGLGISSEGRLYVKELRPEDNAVVLSDNAALFT
                     RVLTADRLNLIPTDSLSAPIRAKARIRYRQQEQPCTIEQ"
     gene            1245..>2881
                     /gene="nrdD"
                     /locus_tag="ACZPAQ_06010"
     CDS             1245..>2881
                     /gene="nrdD"
                     /locus_tag="ACZPAQ_06010"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR0903576.1"
                     /translation="MSAIKVIKRDGTIVDYDRSKIKLAIQKANAEVPEEDRIQEARID
                     SIIDSIEARRRQRLLVEDIQDMVEQALVAENKFLLAKTYIIYRYTRALVRKQNTTDES
                     ILSLLRNENKELAEENSNKNTMIAATQRDYIAGEVSRDLTRRILLPEYISKAHDEGAI
                     HFHDADYFIQPIFNCCLINIGDMLDNGTVMNGKLIESPKSFQVACTVTTQIIACVASN
                     QYGGQSVDMSHLGKYLRRSKEKFRKHITYECAGQVDDATIERLVADRLKDELKSGVQT
                     IQYQINTLMTTNGQSPFVTLFLNLQEGDPYLEENAMIVEEVLRQRLEGIKNEKGVYIT
                     PAFPKLVYVLDEHNCLKGGKYDYITELAVKCSAKRMYPDYISAKKMRENYEGNVFSPM
                     GCRSFLSPWKDENGNYKFEGRFNQGVVSLNLPQIGILARGDEEKFWQLLDQRLQLCFE
                     ALMCRHNALKNVRSDSSPIHWQYGAIARLPKGAPIEPLLYGGYSSISLGYIGLYEVTK
                     LVKGCSQTTPEGQAFSLKVIERLRSACDTWKKETNIG"
BASE COUNT          580 a          987 c          820 g          494 t
ORIGIN      
        1 ctgctcaatg gtgcagggct gctcctgctg gcggtagcgg atacgggcct tggcccggat
       61 aggcgcactg aggctgtctg tggggatcag gttcagccgg tccgctgtca ggacgcgggt
      121 gaacagggcg gcgttgtccg acaggaccac cgcattgtcc tccggccgca gctccttcac
      181 atacagccgc ccctccgagg agatccccag tcccctgcgc tggcccaccg tataggagac
      241 gatcccctca tgggttccca gacgcctgcc gttctgatcc aggaagggcc ccggagggta
      301 ctccctcccc atgtaccggc ggaggaaagc ggcataatcc ccgtctggga tgaagcagat
      361 atcctggctc tccttcttct ccgcgttgcc gaagccctgc cgtgcggcaa tggcccggat
      421 ctcctccttg gacagccccc ccagggggaa tcggctgcgg gacagctgct cctgggtcag
      481 gctgtacaac acgtagctct ggtccttctc cgggtggagg gcctttttca gcagacagcg
      541 gccggtggcg ccgtcccgct ccatgcgcac atagtggccg gtggccaccg cctccgcccc
      601 cagctccagg gacctgcgga gcagctcccc gaacttgatc ccccggttgc agtccacgca
      661 ggggttaggg gtgcagcccg cctcataggc cgccgcgaag gggcggatca cccgctcccg
      721 gaagcaggtc cggcagtcca gcacctggtg gggcatcccc agccgctccg ccaccgcccg
      781 agcgtccgcc aggtcgtcac tggcgccgca tgtcccttcc ggcgtctccc ggtcgaacag
      841 cctcagggtc acccccatca gctcatgccc ctcccgctgg agcagccatg ccgccaccga
      901 gctgtccact cctccgctca tcgccactgc gatccgctct cccatgggtc tctccttccc
      961 agatgaaaat cgtcaaaacg ctgaaaattt gagtccagta tatcacgttt ttcccgccgg
     1021 ggcaacctcc cgccggcgga aaactcccgc accccccgat tttccgccct ttccctggca
     1081 aaacagattt tgtggtcttt ttttgttttt aaaaaaatca cccacaagat atagtggaca
     1141 ccacatgccg cttatggtat aatcagggcc cggcagagtt ttatgcgggc ggccccagcc
     1201 cccgctcacc cacttacaaa ggattcaagg gagaggagta cgccatgagc gccatcaaag
     1261 tcatcaagcg ggacgggacc atcgtggact acgaccgcag caagatcaag ctcgccatcc
     1321 aaaaggccaa cgccgaggtc ccggaggagg accgcatcca ggaggcgcgc atcgactcca
     1381 tcatcgacag catcgaggcc cgcaggcgcc agcgcctgtt ggtggaggac atccaggaca
     1441 tggtggagca ggccctggta gcggagaata agttcctgct ggccaagacc tacatcatct
     1501 accgctatac ccgcgccctg gtccgtaagc agaacaccac cgacgagtcc atcctctccc
     1561 tgctgcgcaa cgagaacaag gagctggcgg aggagaactc caacaagaac accatgatcg
     1621 ctgctaccca gcgggactac atcgccggcg aggtgagccg ggacctgacc cggcgcatcc
     1681 tgctgcccga gtacatctcc aaggcccacg acgagggagc catccacttc cacgacgcgg
     1741 actatttcat ccagcccatc ttcaactgct gcctcatcaa catcggggac atgctggaca
     1801 acggcaccgt gatgaacggc aagctcattg agagccccaa gagcttccag gtggcctgca
     1861 ccgtcaccac ccagatcatc gcctgtgtgg cctccaacca gtacggcggg cagtccgtgg
     1921 atatgagcca cctgggcaag tacctgcgcc gcagcaagga gaagttcaga aagcacatca
     1981 cctatgagtg cgccggtcag gtggacgacg ccaccatcga gcgactggtg gccgaccggc
     2041 tgaaggacga gctgaagagc ggcgttcaga ccatccagta ccagatcaac accctgatga
     2101 ccaccaacgg ccagtccccc ttcgtcaccc tgttcctgaa ccttcaggaa ggcgacccct
     2161 atctggagga gaacgccatg atcgtagagg aggtgctgcg ccagcggctg gagggcatca
     2221 agaacgagaa gggggtctac atcacccccg ctttccccaa gctggtgtac gtgctggacg
     2281 agcacaactg cctgaagggc ggcaagtatg actacattac cgagctggcc gtcaagtgct
     2341 ccgccaagcg gatgtacccc gactatatct ccgccaagaa gatgcgggag aactacgagg
     2401 gcaacgtgtt ctcccccatg ggctgccgct ccttcctctc cccctggaag gacgagaatg
     2461 ggaactacaa gttcgagggc cgcttcaacc agggcgtggt ctccctgaac ctgccccaga
     2521 tcggcatcct ggcccggggg gatgaggaga agttctggca gctgctggac cagcggctcc
     2581 agctgtgctt cgaggccctc atgtgccgcc acaacgccct gaagaacgtg cgctccgact
     2641 ccagccccat ccactggcag tacggcgcca ttgcccgcct gcccaagggg gcccccatcg
     2701 agcccctgct ctacggcggc tactcctcca tctccctggg ctatatcggc ctgtacgagg
     2761 tgaccaagct ggtgaagggc tgctcccaga ccacccctga gggacaggcc ttctccctga
     2821 aggtgattga gcggctgcgc tccgcctgcg acacctggaa gaaggagacc aacatcggct
     2881 t
//
LOCUS       DBMDSJ010000219         2877 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8485_length_2877_cov_1.784942, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000219 DBMDSJ010000000
VERSION     DBMDSJ010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8485_length_2877_cov_1.784942"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="ACZPAQ_06015"
     CDS             complement(<1..448)
                     /locus_tag="ACZPAQ_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR0903577.1"
                     /translation="MNIKIALAGNPNCGKTTLFNALTGSNQYVGNWPGVTVEKKEGKL
                     KGHKEVIIQDLPGIYSLSPYTLEEVVARNYLVNEKPDAILNIVDGTNIERNLYLTTQL
                     IELGLPVVVAVNMIDLVRKNGDKIDLQKLGQALGCQVVEMSALKGEG"
     gene            complement(510..731)
                     /locus_tag="ACZPAQ_06020"
     CDS             complement(510..731)
                     /locus_tag="ACZPAQ_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005927392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR0903578.1"
                     /translation="MKTLKDAKVGDTVTVAKLHGEGAVKRRIMDMGITKGVEIHIRKV
                     APLGDPMELNVRGYELSVRKADAEMIEIL"
     gene            complement(746..958)
                     /locus_tag="ACZPAQ_06025"
     CDS             complement(746..958)
                     /locus_tag="ACZPAQ_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR0903579.1"
                     /translation="MMPLTMARPGETVVIRKITGRDEVRQHLAELGFVVDESVTVISE
                     MAGNLILQVKDSRVAVDRTMANRIMI"
     gene            complement(1214..>2877)
                     /locus_tag="ACZPAQ_06030"
     CDS             complement(1214..>2877)
                     /locus_tag="ACZPAQ_06030"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903580.1"
                     /translation="TSKIFRNSFLVGVAVFFLSIALFMGVLYQYFGSQLLIQLESEAA
                     LAARGVEMGSMDYLDGLSSANRITWIDAGGTVLFDNQADPAQMENHADREEVRAALES
                     ETGTASRYSTTLSQRTLYFAQRLADGTVLRVSSEQRSLPSLLLSMVQPILIILVLAVA
                     LSAVLASRLSKHIIKPVLAIDLEHPEDSDTYEELTPLLSRIKQQNQTIAQQMAQLRQK
                     QNEFTAITENMSEGFLLLDRQGRILSHNSGALRLLHAAAPEERASYLTLNRTERFRQA
                     VDAALTGRRDEQLVDLGGRCCQLLANPVIQDGQVAGAVLVILDVTEREQREELRREFT
                     ANVSHELKTPLTSISGIAEIIKGGLVKPQDIPGFADDIYGEAQRMIRLVEDILRLSQL
                     DEGGSSLQTEEVDLYHLAQEVAGRLTPAAVKGQVTLEVTGGPVRISGVRQLLDEMISN
                     LCDNAIKYNRPGGRVTISVSRSDRGAELSVSDTGIGIPPEDQSRVFERFYRVDKSHSK
                     AIGGTGLGLSIVKHGAAFHGADLSLESQPDRGTTIRLTFPGQNVK"
BASE COUNT          517 a          875 c          906 g          579 t
ORIGIN      
        1 cgccctcgcc cttcagggcg ctcatctcca ccacctggca gcccagggcc tggcccagct
       61 tctggaggtc aatcttgtcg ccgttcttcc gcaccagatc tatcatgttg accgccacta
      121 ccacgggcag acccagctcg atgagctggg tggtcaggta caggttgcgc tcgatgttgg
      181 tgccgtccac gatgttgagg atggcgtccg gcttctcatt gaccaggtag ttgcgggcca
      241 ccacctcctc cagcgtatag ggggacaggg agtagatgcc aggcaggtcc tggatgatga
      301 cctccttgtg gcccttcagc ttgccctcct tcttttccac cgtgacgccg ggccagttgc
      361 ccacgtactg gttggagccg gtcagggcat tgaacagggt ggtcttaccg cagttcgggt
      421 tgcccgccag cgcgatcttg atgttcatgg tatttctccc ttcagaaagt tagaagtgac
      481 taactaacag tgaaaaacga aagcggagct cagaggatct cgatcatctc cgcgtctgcc
      541 ttgcggacgg acagctcata gccccgcaca ttcaactcca tagggtcccc cagcggggcg
      601 acctttcgga tgtggatctc cactcccttg gtgatcccca tatccataat gcggcgcttc
      661 acggctccct cgccgtggag cttagccacg gtgacggtgt ctcccacctt ggcgtctttc
      721 agcgttttca tcctttctct cctcctcaaa tcataatacg gttggccatg gtcctgtcca
      781 cagcgacccg gctgtccttt acctgaagga tcaggttgcc ggccatctcg ctgatgacgg
      841 tcaccgactc gtccaccaca aagccgagct ctgccagatg ctggcgcacc tcgtccctgc
      901 cggtgatctt tcggatgacc acggtctcgc cgggtctggc cattgtcagg ggcatcataa
      961 gactccctcc cagatcaggt tgacttttgc tgctagaatt gcttgaaaag ctggttagct
     1021 taatctaacc atcggaacag atagagttta cttctgctaa ccggatttgt caagcccttt
     1081 tctgaaaaaa ttgggccgtg ctccgcactt ttccagccgc gacaaaagca gccggtgaac
     1141 agagcaggat cccgtctatt tttcccagag agaaaaaagc atggcccgcc gtaccagcgg
     1201 cgggccatgt gccttatttc acgttttggc cggggaaggt gaggcggatg gtggtcccgc
     1261 ggtccggctg gctctccagg gagaggtcgg ccccgtggaa ggccgccccg tgcttgacaa
     1321 tggacagtcc cagaccggtg cctccgatgg ctttggagtg gctcttgtcc acccggtaga
     1381 accgctcaaa gacccggctc tggtcctcag gagggatacc gatgcccgtg tcggacacag
     1441 agagctccgc accccggtcg gagcgggaga cggagatagt gacccggccc ccaggacgat
     1501 tgtacttgat ggcgttgtcg cacaggttgc tgatcatctc gtccaaaagc tggcgcacgc
     1561 cagagatccg caccggtccg cccgtcacct ccagggtgac ctgccccttg acagcggcgg
     1621 gggtcaggcg gccggccacc tcctgggcca ggtggtacag gtccacctcc tccgtctgga
     1681 gggagctgcc cccttcatcc agctgggaca ggcggaggat gtcctccacc aggcggatca
     1741 tgcgctgggc ctcgccgtag atgtcgtctg cgaagccggg gatgtcctgc ggcttgacca
     1801 gcccgccctt gatgatctcc gcaatgccgg agatggaggt gaggggggtc ttgagctcat
     1861 gagatacatt ggcggtgaac tcccggcgca gctcctcccg ctgttcccgc tcggtgacat
     1921 ccaggatgac cagcacggcg ccggccacct ggccgtcttg gatcacgggg ttggccagca
     1981 gctggcagca gcggcctccc aggtccacca gctgctcgtc ccgcctgccg gtgagggcgg
     2041 catccaccgc ttggcggaag cgctcggtgc ggttcagagt gaggtagctg gcccgttcct
     2101 caggggcggc ggcgtggagc aggcgcaggg caccggagtt gtgggagagg atgcgcccct
     2161 gccggtccag caggaggaag ccctcgctca tgttctcggt gatggcggtg aactcgttct
     2221 gcttctggcg cagctgggcc atctgctggg cgatggtctg gttctgctgc ttgatgcggg
     2281 aaagcagggg ggtgagctct tcgtaggtgt cggagtcctc tgggtgctcc aggtcgatgg
     2341 caaggacggg cttgatgatg tgcttggaaa ggcgggaggc cagcaccgcc gacagggcca
     2401 cggccagcac caggatgatg aggatgggct ggaccatgga caggagcagg gaagggaggc
     2461 tgcgctgctc actggacacc cgtagcaccg tcccgtcagc cagccgctgg gcaaagtaca
     2521 gggtccgctg agagagggtc gtggagtagc gggaggcggt gcctgtctcg ctctccagag
     2581 cggctcgaac ctcctcccgg tcggcgtggt tctccatctg ggcggggtcg gcctggttgt
     2641 caaagaggac agtgccgccg gcgtcgatcc aggtgatacg gttggcggag gagagaccgt
     2701 ccagataatc catgctgccc atctccaccc cacgggcggc cagggcggcc tccgactcca
     2761 gctggatcag cagctggctg ccaaaatact ggtacagcac gcccatgaac agagcgatag
     2821 agagaaagaa caccgcaacg cccaccagaa aggagttgcg gaagatttta gatgtca
//
LOCUS       DBMDSJ010000220         2863 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8536_length_2863_cov_2.069515, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000220 DBMDSJ010000000
VERSION     DBMDSJ010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_2863_cov_2.069515"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="ACZPAQ_06035"
     CDS             complement(<1..884)
                     /locus_tag="ACZPAQ_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0903581.1"
                     /translation="MNASIDVLTLFLFNIVLVNIPLLYGTVGEIVTEKAGSLNLGVEG
                     TMAIGAIFGYLAGCMANSLLVGLLVSFLAAGLCGALFALLTVSLQANQNVTGLTITTF
                     GLGLYFFIGKGLGDRWPVMTSADALVSGFSAREIPLLSSIPVVGKALFSHNILVYLGI
                     LVAAAVWWYLTCTKTGLRLRSVGENPGAADSVGVNILLYKYVHLILGSGIMGLGGFYM
                     ALNMGGSFEGSNCWINGYGWIAIALVIFANWNPLYAILGTLVFGFFNTLQIYSGSLAG
                     AFPDALGWLNSIPSHLFKA"
     gene            complement(881..2008)
                     /locus_tag="ACZPAQ_06040"
     CDS             complement(881..2008)
                     /locus_tag="ACZPAQ_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0903582.1"
                     /translation="MNRKEPLFRVVKRESSSPVRQALAYVAAVVIALLIGAALLSSMD
                     VDPLTFYQKMVTMGIPGNKFPWRIVENFVKLFVPLLITSLALSLAFRMRFWNIGGEGQ
                     FIMGAICAAAAAHKLGPVLPQPLVVLVMALCGILGGGLYGLIVALLKVRYNTNETLLT
                     LMLNYIALYLLQFLGETKAGWNFFLDPDSARPKFAGFPDNADMITFPLGPFNLNLSLI
                     IAIVLCVLLFGYLKYTKHGYEIAVVGDSPNTARYAGMKVGRIVVRTIFLSAALIGLAG
                     AFYVSSAGTLSTSVTNDVGWTGIVVAWLAKLNTVGIVITALLITVLQFGCQAASTTYS
                     SIDANFADLIQGIILFAVLAADFFTRFRLARRAAAPKEVSK"
     gene            complement(2005..>2863)
                     /locus_tag="ACZPAQ_06045"
     CDS             complement(2005..>2863)
                     /locus_tag="ACZPAQ_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0903583.1"
                     /translation="KVDLNIERPVVEKTRPLLEIRDLTIRSDEGAVAIDRVNFYIRGG
                     EILGVAGIAGCGQKELCEAIAGLRPIEGGQMIHKGENIVGLSPKAILDKGISMSFIPE
                     DRLGMGLAPSLSITDNMLLKTYSDGRGIFVDRKRGRAEAEHVIQELEVVTPSTETPVR
                     RLSGGNVQKVLLGREIKAGPNVIVTAYPVRGLDINSSYAIYHILNEQKKSGVGILFVG
                     EDLDVMMALCDKIMVLCHGRVMGVVHAHKVTKEELGLMMTGALDLTRKYEDKPAGIAR
                     DTLIEEVDQ"
BASE COUNT          551 a          843 c         1002 g          467 t
ORIGIN      
        1 gccttgaaga ggtgggaggg gatgctgttg agccagccca gagcgtccgg gaatgcgccg
       61 gccagagagc cggagtagat ctgaagggtg ttgaagaagc caaagaccag ggtgcccagg
      121 atggcataca gggggttcca gttggcgaag atgaccaggg cgatggcgat ccagccgtag
      181 ccgttgatcc agcagttgga gccctcgaag gagccgccca tgttcagtgc catatagaag
      241 ccgcccagcc ccatgatgcc ggagcccagg atcaggtgga cgtacttata cagcaggatg
      301 ttgacgccca ccgagtcggc agcgccgggg ttctccccca cggagcgcag gcgcaggccg
      361 gtcttggtgc aggtgaggta ccaccacacc gccgccgcca ccaagatgcc cagatagacc
      421 aggatgttgt gggaaaacag ggccttgccc accaccggga tggaggagag cagggggatc
      481 tcccgggcgg agaagccgct gaccagggcg tcggcgcttg tcatgacggg ccagcggtcc
      541 cccaggccct tgccgatgaa gaagtagagg cccaggccga aggtggtgat ggtcaggccg
      601 gtgacgttct ggttggcctg gagggagacg gtgagcaggg caaacagggc cccgcacagg
      661 ccggcggcca gaaaggagac cagcaggccc accagcaggc tgttggccat acagcctgcc
      721 aggtagccga agatggcgcc gatggccatg gtgccctcca cgcccagatt caggctgcca
      781 gccttctcgg tgacgatctc gcccactgtg ccgtagagca gggggatgtt caccagcacg
      841 atgttgaaca ggaagagggt caatacgtcg atggatgcgt tcatttggac acctccttgg
      901 gggctgcggc gcgccgcgcc agacggaagc gggtgaagaa gtccgcggcc aggacggcga
      961 acaggatgat gccctggatc aggtccgcga agttggcgtc gatggaggaa taggtggtgc
     1021 tggcagcctg acagccgaac tggagcactg tgatgagcag ggcggtgatg acgatgccca
     1081 ccgtgttcag cttggccagc caggccacca cgatgccggt ccagcccaca tcgttggtga
     1141 cggaggtgga cagggtgccg gcggaggaga catagaaggc gccggccagg ccgatgaggg
     1201 cagcggacag gaagatggtg cgcaccacga tccgccccac cttcatgccc gcatagcggg
     1261 cggtgttggg gctgtccccc accacggcga tctcatagcc gtgcttggtg tacttcagat
     1321 agccgaagag gagcacgcac aggacgatgg cgatgatcag ggacaggttc aggttgaagg
     1381 ggcccagggg gaaggtgatc atgtccgcgt tgtcggggaa gccggcgaac ttgggtcggg
     1441 cggaatcggg gtccaggaag aagttccagc cagccttggt ctcccccagg aactggagga
     1501 gatagagagc gatgtagttg agcatcaggg tgagcagggt ctcgttggtg ttgtagcgca
     1561 ccttcagcag ggccacgatc aggccgtaca ggccgccgcc caggatgccg cacagggcca
     1621 tgaccagcac caccaggggc tggggcagga ccgggcccag cttgtgggcg gccgcggcgg
     1681 cacagatggc gcccatgatg aactgcccct ctccgccgat gttccagaag cgcatccgga
     1741 aggccagcga cagggccagg gaggtgatga gcagggggac aaagagcttg acgaagttct
     1801 ccacgatgcg ccaggggaat ttgttgcccg ggatgcccat ggtcaccatc ttctggtaga
     1861 aggtgagggg gtccacatcc atggaggaga ggagggccgc gccgatgagc agggcgatga
     1921 ccaccgccgc cacataggcc agggcctggc ggacggggct ggacgactcc cgcttgacca
     1981 cgcggaagag cggttctttc cggttcattg gtccacctcc tcgatcagag tgtcccgggc
     2041 aatgcccgcg ggcttgtcct catacttgcg ggtcaggtcc agggcgccgg tcatcatcag
     2101 gcccagctcc tccttggtga ccttgtgggc gtggactacg cccatcactc tgccgtggca
     2161 gaggaccatg atcttgtcgc acagggccat catcacgtcc aggtcctcgc ccacgaacag
     2221 gatgcccacg ccggacttct tctgctcgtt gagaatgtgg taaatagcat aggaagagtt
     2281 gatgtccagg ccgcggacag gataggcggt gacgatgacg ttggggccgg ccttgatctc
     2341 gcggcccagc agcacctttt gcacgttgcc gccggacagg cggcgcaccg gggtctcggt
     2401 ggagggggtg accacctcca gctcctggat gacgtgttcc gcctccgctc ggccccgctt
     2461 ccggtccaca aagatgcccc tgccgtcgct gtatgttttc agcagcatgt tgtccgtgat
     2521 ggacagggag ggggccagac ccatgcccag ccggtcctcc gggatgaagg acatggagat
     2581 gcccttgtcc aggatcgcct tgggggagag gcccacgatg ttctccccct tgtggatcat
     2641 ctggccgccc tcgatgggcc gcaggccggc gatggcctca cacagctcct tctggccgca
     2701 gcccgcgatg ccggccacgc ccaggatctc gccgccccgg atatagaagt tcacccggtc
     2761 gatggccacg gcaccctcgt cgctgcggat ggtcaggtcc cggatctcca gcagggggcg
     2821 ggtcttttcc accacggggc gctcgatgtt caggtccacc ttg
//
LOCUS       DBMDSJ010000221         2863 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8537_length_2863_cov_1.503621, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000221 DBMDSJ010000000
VERSION     DBMDSJ010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8537_length_2863_cov_1.503621"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            100..723
                     /locus_tag="ACZPAQ_06050"
     CDS             100..723
                     /locus_tag="ACZPAQ_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HIR0903584.1"
                     /translation="MYRDQCSKTLYRTPLQPGWWLEGNQAMKSLKLLMFAALMVAMAR
                     ALSLIPGIPIAHTKMTWGFLARALCALVCGPVMGLVFGFVEDILGFILQPTGDFFPGY
                     TLSTMAGVLVYALCFYRARITVLRIVLANLVVNLLVNALMGSLWNLMLRGGVYWGWFL
                     PSLGKNLVTVLPKTLVIYVLFQALLPILQQMGVIPRQLDEKGRLPLI"
     assembly_gap    848..857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <858..>2863
                     /locus_tag="ACZPAQ_06055"
     CDS             <858..>2863
                     /locus_tag="ACZPAQ_06055"
                     /inference="COORDINATES: protein motif:HMM:NF015714.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR0903585.1"
                     /translation="LLPLSCALAALCAVALLCLRGRARVRGALICGGLALGLLWTAAY
                     GAVFYAPARALDDRTIRLSGTVMEYPQETDYGWSVLVRADGEPGAPVPTLVYADEQGA
                     DLRPGDRVETVAHCTLAERTFGGEEITYYTAKGIFLRAEAYGELRVTRPERLPLRYLP
                     AELAHQLKQGVDAAFPEDAAPLIRALTTGNRDNLTDHYTSSLQRTGLSHTVAVSGMHL
                     SFLAALVSGLLGRGKRRSALVTCGVVLVFTLISGCTPSVVRAAVMLILLQLAALLGRE
                     RDPLTALALALMLLLAWNPFSAAHIGLQLSFASVAGILLFSDRLQERMLRGWKRPPKD
                     SIPLRLLGALVRFGAAALSATLGAMVFTTPLTALYFGSLSLISPLANLMTLWAVSAAF
                     SGGLIAGAAGAVLPELGRVLALPVLPFVRYLDWVVPRLARAPFSALSTSSFYYLAWLL
                     FVYVLLLLGLLYRGKKGWKTPVCACLAALCASLLFHARSFQAGDMTAAVLDVGQGQSV
                     LIRLGDHYTLVDCGGDGPDDPGDTAADCLQGLGRDRLDLLVLTHFHDDHANGVPELLE
                     RLSVSAIALPDVEEDSPLRREILTLAEEKGIPLWWVRRDTTVELEKGQLTLYPPLGAG
                     EANELGLSVLAGAGGLDLLVTGDMSGDVEGMLVEHAGLRDVELL"
BASE COUNT          381 a          918 c         1028 g          526 t
ORIGIN      
        1 atcccgcatc ccgctgccgc ataccggggc gggacctccg cttcctctgt gcggcaatcc
       61 cgaaagggct gccggatcgc agaaggaggc gttttcttta tgtatcgaga ccaatgttcc
      121 aagaccctgt accgcacccc gctccagccc ggctggtggc tggaggggaa tcaggccatg
      181 aaaagcctga agctgctgat gttcgcggca ctgatggtgg cgatggcccg ggcgctgtcc
      241 ctcatccccg gcatccccat cgcccacacc aagatgacct ggggctttct ggcccgggcg
      301 ctgtgcgccc tggtctgcgg gccggtgatg ggactggtgt tcggctttgt ggaggatatc
      361 ctgggcttta tcctccagcc caccggggat ttcttccctg gctacaccct gtctaccatg
      421 gcaggggtgc tggtctatgc cctgtgcttt taccgggcgc ggatcacggt gctgcgcatc
      481 gtgctggcca acctggtggt gaacctgctg gtcaacgccc tgatgggctc cctgtggaac
      541 ctgatgctcc ggggcggggt ctactggggg tggttcttgc ccagcctggg caagaacctg
      601 gtgaccgtcc tgcccaagac cctggtgata tatgtactgt tccaggcgct gctgcccatc
      661 ctccagcaga tgggggtcat ccccagacag ctggatgaga aggggcggct ccccctgatc
      721 tgagagaacg gacaaaaggc ggacagagag gaggagcggg ctgtgcgggt gctggcaacg
      781 gcggcgggct ccttctctct ggccgtcttt gccgccaact gcatcccgct gctggagggg
      841 gcgctgcnnn nnnnnnncgc tgctcccgct gagctgtgcc ctggcggcgc tgtgcgccgt
      901 ggccctcctg tgcctccgtg ggcgggcacg agtaagaggg gccctgatct gcggcgggct
      961 ggccctggga ctgctgtgga cggcggccta tggggcggtg ttttatgccc cggcccgagc
     1021 gctggacgac aggaccatcc ggctgtccgg cacggtgatg gagtaccccc aggagaccga
     1081 ctacggctgg tcagtgctgg tccgggcgga cggggagccg ggggccccgg tgcccacgct
     1141 ggtctatgcg gacgaacagg gggcggacct gcggccaggg gaccgggtgg agacggtggc
     1201 ccactgcacc ctggcagagc ggaccttcgg cggggaggag atcacctact acaccgcaaa
     1261 gggcatcttt ctgcgggcgg aggcttacgg cgaactgcgg gtgacgcggc cggagcggct
     1321 cccgctgcgg tatctcccgg cggagctggc ccaccagctc aaacaggggg tggacgccgc
     1381 cttcccagag gatgcggcgc ccctgatccg ggcactgacc accgggaacc gggacaacct
     1441 caccgaccac tacacctcct cccttcagcg aaccggcctg tcccacacgg tggcggtgtc
     1501 cgggatgcac ctgtccttcc tggccgccct ggtctccggc ctgctgggcc ggggaaagcg
     1561 gcgctccgcc ctggtcacct gcggggtggt gctggtcttt acgctcattt ccggctgcac
     1621 gccctcagtg gtgcgggcgg cggtgatgct gatcctgctg caactggccg ccctgctggg
     1681 gcgggagcgg gacccgctga cggccctcgc cctggccttg atgctcctgc tggcctggaa
     1741 ccccttttcc gcagcccata tcggacttca gctctccttt gcatcggtgg cgggcatcct
     1801 gcttttttca gaccggctcc aggagcggat gctgcggggg tggaagcggc cgcccaagga
     1861 ctccatcccg ctccggctgc tgggggcgct ggtccgcttc ggggcggcgg ccctgtccgc
     1921 caccctgggg gccatggtgt tcaccacgcc gttgacggcc ctctatttcg ggagcctgtc
     1981 cctgatctcc cccctggcta atctgatgac cctgtgggcc gtgtctgcgg ccttcagcgg
     2041 cgggctgatc gctggagcgg caggggcagt gctgccggaa ctgggcaggg tgctggctct
     2101 cccggtgctc ccctttgtcc gctatctgga ctgggtggtg ccccggctgg cccgggcccc
     2161 cttctcggca ctgagcacca gctccttcta ctatctggcc tggctgctgt tcgtctatgt
     2221 cctgctgctc ctggggctgc tgtaccgggg gaagaaggga tggaagaccc ctgtctgtgc
     2281 ctgcctggct gcgctgtgcg ccagcctgct gttccacgcc cggagctttc aggcggggga
     2341 tatgacggcg gcggtgctgg atgtggggca ggggcagagc gtcctgattc ggctggggga
     2401 ccactacacc ctggtggact gcggcgggga cggaccggac gaccccgggg acacggcagc
     2461 ggactgcctt caggggctgg ggcgggaccg gctggatctg ctggtgctca cccacttcca
     2521 cgacgaccac gccaacggcg tgcccgagct gctggagcgg ctgtcggtgt ccgccatcgc
     2581 cctgccggac gtggaggagg acagccccct gcggcgggag atcctgacgc tggcggagga
     2641 gaagggcatc ccgctgtggt gggtccgccg ggataccaca gtggagctgg agaaggggca
     2701 gctgacgctg tatccccctc tgggagcggg agaggccaac gagctggggc tgtccgtgct
     2761 ggccggcgcc ggcggactgg acctgctggt caccggagat atgagcgggg atgtggaggg
     2821 catgctggtg gagcacgcgg ggctccggga cgtggagctg ctg
//
LOCUS       DBMDSJ010000222         2858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8559_length_2858_cov_1.636561, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000222 DBMDSJ010000000
VERSION     DBMDSJ010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_2858_cov_1.636561"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /gene="trxA"
                     /locus_tag="ACZPAQ_06060"
     CDS             complement(<1..262)
                     /gene="trxA"
                     /locus_tag="ACZPAQ_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR0903586.1"
                     /translation="MSLKHFTRETFEKELNGGALMLVDFWAEWCGPCRMLGPVIEGLA
                     DEYEGRAVIGKVNVDEEGELAMRYGVMNIPTVILFKNGREIDR"
     gene            complement(284..1144)
                     /locus_tag="ACZPAQ_06065"
     CDS             complement(284..1144)
                     /locus_tag="ACZPAQ_06065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0903587.1"
                     /translation="MNYDVLVVGSGPAGLAAAVAARGRDKSVLVVGNPWQESPLARAE
                     RIDNYLGLPGVNGRELMEHFSAHAQRVGVEFHTGRVVAVMAWEGFALTAGSEVFQGRT
                     LVLAPGVARLAKYPGEAELLGRGVSYCATCDGMLYRGRAVTVVGRSKDAPQEANYLKS
                     LGCQVVYVSPQEPKGLDREIPFVRAGKLEVRGTDRVSALVADGAELPCEGVFILRDAL
                     APTDLLPDLELRNGAIAVDREMATSLPGVFAAGDCTGGPLQVAKAVGEGHTAGLSAAE
                     YVDRKRNEHQ"
     gene            1419..>2858
                     /locus_tag="ACZPAQ_06070"
     CDS             1419..>2858
                     /locus_tag="ACZPAQ_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR0903588.1"
                     /translation="MSDETIISTQSREGPAPSQNFIHEFIAEDIAPGGRFAGMQVHTR
                     FPPEPNGYLHIGHCKALIIDFGSAEKFGGICNLRMDDTNPAKEDTEYVDAIQEDIHWL
                     GFDWGDRFFYGSDYFEKTYELAVGLIKKGLAYVCELTPEQFREYRGDVTTPARSPWRD
                     RPIEESLDLFERMKNGEFPEGRYTLRAKIDLESGNFNMRDPVLYRIRYIEHHRQGTKW
                     CIFPMYDFAHPIQDALEGITHSLCSLEYEAHRPLYDWVIQNTDVPSKPRQIEFARLGI
                     NYTVMSKRKLRKLVEEDYVSGWDDPRMPTLCGLRRRGYTPVSIRNFCDRIGVAKATST
                     VEYAFLEHCLREDLNEHAQRAMAVLHPIKLTITNYPEGQSETLEVENNPNDPGAGTRT
                     VSFSRHLYLESDDFLETPVPKYKRLFPGGPECRLKGAYLITCTGCVKDEAGNVVEVLA
                     EYDPLSRGGDPADGRKVKGATLHWVDAATA"
BASE COUNT          497 a         1038 c          794 g          529 t
ORIGIN      
        1 tgcggtcgat ctccctgccg ttcttgaaga ggatcacggt ggggatgttc atcacgccat
       61 agcgcatggc cagctccccc tcctcgtcca cgttcacctt gccaatgacg gcccggccct
      121 catactcgtc ggccagtccc tcgatcactg ggcccagcat acggcagggg ccgcaccact
      181 ccgcccagaa atccaccagc atcagcgcgc cgccgttcag ttccttctca aaggtctctc
      241 tggtaaaatg cttcaatgac atttttgatc cttcccttct gtctcattgg tgttcattgc
      301 gtttccggtc cacatactcc gccgcgctca ggcccgcggt gtggccctcg cccaccgcct
      361 tggccacctg gagagggccg ccggtgcagt cccccgcagc gaacacgccg ggcaggctgg
      421 tggccatctc ccggtccacc gcaatggccc cgttcctcag ctccagatca gggagcagat
      481 cggtgggggc cagggcgtcc cgaaggatga acaccccctc gcagggcagc tccgcgccgt
      541 cggccaccag ggcgctcact cggtcggtcc cccgcacctc cagctttccg gcccggacga
      601 atgggatctc ccggtccagc cctttgggct cctgggggga gacatagacc acctgacagc
      661 ccaagctttt caggtagttg gcctcctggg gggcgtcctt gctccggccc accactgtga
      721 ccgcccggcc ccggtagagc atcccgtcgc aggtggcgca atagctcacc ccccggccca
      781 gcagctccgc ctcccccggg tacttggcca gccgggccac ccctggcgcc agcaccagag
      841 tgcgcccctg gaacacctcg ctgcccgccg tgagggcaaa cccctcccag gccatcaccg
      901 ccaccacccg gccggtgtgg aactccaccc ccacccgctg ggcatgggcg ctgaagtgct
      961 ccatcagttc ccgtccgttg accccgggca gccccagata gttgtcgatc cgctccgccc
     1021 gggccagggg gctctcctgc cagggattgc ccaccaccag gacgctcttg tcccgtcccc
     1081 gggcggccac agccgccgcc aggcccgccg ggccgctgcc cactaccagc acatcgtagt
     1141 tcaaggccgc gcctcctctc cgcctccggg tgctctcccg gacctgtgtt ctggtttgtt
     1201 ttattatacc tgccttttgt caaaaaggca acgcttttcc gcggcgcgcc aggccggtcc
     1261 cgcccgcttt tctccgaact gaacggagct gtctcctctc ttcccctctt ttctgtatgt
     1321 tcccccggtt ttctcccatt tacgtgggaa tcggttgatt attcctctga aatatggtat
     1381 aaaattagag ttaagtttac gcaagaggag gacccgatat gtccgacgaa accattattt
     1441 ccactcagag cagggaaggc cccgccccca gccagaattt catccatgaa tttatcgcgg
     1501 aggacatcgc ccccggcggc cgctttgccg ggatgcaggt ccacacccgt ttcccgcccg
     1561 agcccaacgg ctatctccac atcggccact gcaaggcgct tatcatcgac ttcggctctg
     1621 ccgagaagtt tggcggcatc tgcaacctgc gcatggatga caccaacccg gccaaggagg
     1681 acaccgagta cgtggacgcc atccaggagg atatccactg gctgggcttc gactggggcg
     1741 accggttctt ctatggctcc gattactttg aaaagactta cgagctggct gtgggcctaa
     1801 tcaagaaggg cctggcctat gtttgtgagc tcacccccga gcagttccgg gagtaccggg
     1861 gcgatgtgac cacccccgcc cgctccccct ggcgtgaccg ccccatcgag gagtctctgg
     1921 acctgtttga gcggatgaag aacggcgagt tccccgaggg caggtacacc ctgcgggcca
     1981 agatcgacct ggagagcggc aacttcaaca tgcgggaccc ggtgctctac cgcatccgct
     2041 acatcgagca ccaccgccag ggcaccaagt ggtgcatctt ccccatgtac gacttcgccc
     2101 accccatcca ggatgccctg gagggcatca cccactccct gtgctccctg gagtatgagg
     2161 ctcaccgccc cctgtacgac tgggtcatcc aaaacaccga cgtccccagc aagccccggc
     2221 agatcgagtt tgcccgcctg ggcatcaact atacagtcat gtccaagcgg aagctgcgca
     2281 agctggtgga ggaggactac gtctccggtt gggacgaccc ccgcatgccc accctgtgcg
     2341 gcctgcgccg acggggctat acccccgtct ccatccgcaa tttctgcgac cgcatcggcg
     2401 tggcgaaggc cacctccacc gtggagtatg cctttttgga acactgcctg cgggaggatc
     2461 tgaacgagca tgcccagcgg gccatggcgg tgctccatcc catcaagctg accatcacaa
     2521 actaccccga gggtcagagc gagaccctgg aggtggagaa caaccccaac gatcccggcg
     2581 ccggcacccg cacagtctcc ttctcccgtc acctgtatct ggagtccgat gactttctgg
     2641 agaccccggt ccccaagtac aagcgtctct tccccggcgg gcctgagtgc cgcctgaagg
     2701 gggcctacct catcacctgc accggctgcg tcaaggacga ggcgggcaac gtggtggaag
     2761 tgctggcgga gtatgatccc ctctcccggg gcggagaccc cgccgacggc cgcaaggtga
     2821 agggggccac cctccactgg gtggacgccg ccaccgcc
//
LOCUS       DBMDSJ010000223         2836 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8624_length_2836_cov_1.667642, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000223 DBMDSJ010000000
VERSION     DBMDSJ010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8624_length_2836_cov_1.667642"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="ACZPAQ_06075"
     CDS             complement(<1..1133)
                     /locus_tag="ACZPAQ_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR0903589.1"
                     /translation="MNHSPAFRRMLAPVLALALVCTLSLPASAFFWNKKTDVPSVSDF
                     SKNGMVGQVISFSAGDFPVSGGSGGTALAAITLKTLPDIGTGMLTVGGQPLTEGALVD
                     ASALSGLQFQALPTPSVNETAFTFLPSFTTGETGEEVTVTLYLLTEANEAPIARNMDL
                     STYKNVAITGWFDAEDGEGDSLTFQLTSTPARGAVTVAEDGSSQFVYTPYENKTGKDS
                     FTYVAVDPAGNVSPEAKVSIQIEKPDTKVTYADMEGNPAHKASIRLAEEGIFVGSYVN
                     GSYFFDPDQPVSRAEFLTMAMAATGLEPLEDVTLTGFYDDEAIPTWAKGYVSSALKAG
                     AIQGSLDQEGQPVFGSGETVTRGEATVMLDNLMGILDVPAEVFA"
     gene            1575..2435
                     /locus_tag="ACZPAQ_06080"
     CDS             1575..2435
                     /locus_tag="ACZPAQ_06080"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR0903590.1"
                     /translation="MKTGLVLEGGALRTIFSSGVCDALLDGGVMVDYVIGVSAGIAYG
                     VSYISRQPRRNLEIVTRYAGDRRYMGMNNLADRHNRSYFGLEFVYDTIPNELVPFDYQ
                     TFAAYPGQVEAVVTNLSTGEAEYMPVPREDDKFLLLQATCALPLMFPIYHLNGQPYLD
                     GGVADSIPYERAFQQGCDRVVVVLSKTRSYVRRPEKLQPVIERTYREYPNFCRAMARR
                     AEQYNTCRERLFQLEREGRVLVLAPVSTHKVSRIERDTKKLRMLWGDGYQQAVDRMDE
                     IRAFLTPDRA"
     gene            complement(2416..2622)
                     /locus_tag="ACZPAQ_06085"
     CDS             complement(2416..2622)
                     /locus_tag="ACZPAQ_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903591.1"
                     /translation="MSHMFSRNSYILLAAAVLLAVLLAWEADVDERGRMAAFFTVLGD
                     ALALLALQPSCPAAEEDEVTPCPA"
BASE COUNT          579 a          803 c          952 g          502 t
ORIGIN      
        1 gcaaagacct ccgcgggcac gtccaggatg cccatgaggt tatccagcat caccgtggcc
       61 tccccccggg tcaccgtctc accggagccg aagacgggct ggccctcctg gtccaaactg
      121 ccttggatag cccctgcctt cagggcagag gagacatagc ccttggccca ggtggggatg
      181 gcctcgtcgt catagaagcc ggtgagggtc acgtcctcca ggggttccag gccggtggcc
      241 gccatggcca tggtcagaaa ttccgcccgg ctgaccggct ggtctgggtc aaagaaatag
      301 cttccgttga cataggagcc cacaaagatg ccctcttcgg ccaggcggat ggaggccttg
      361 tgggcagggt tgccctccat atcagcgtag gtcaccttgg tgtccggctt ttcgatctgg
      421 atggatacct tggcctcggg agatacgttg cccgccgggt ccaccgccac ataggtgaaa
      481 ctatccttgc cagtcttgtt ctcatagggg gtatagacga actggctgga gccgtcctcc
      541 gccacagtca cggccccccg ggcgggggtg gaggtgagct ggaaggtgag ggagtcccct
      601 tcgccgtcct ccgcgtcgaa ccagccggtg atagccacat ttttataggt ggacaggtcc
      661 atattccggg cgatgggggc ctcgttggcc tcggtgagca gatatagggt gacggtcacc
      721 tcctcaccgg tctctccggt ggtaaaggag ggaaggaagg tgaaggcagt ctcgttgaca
      781 gagggggtgg gcagagcttg gaactggagc ccgctcaggg cggaggcgtc caccagggcg
      841 ccctcggtca ggggctgtcc tcctacggtg agcatccctg tgccgatatc gggcagagtt
      901 ttcagcgtaa tggcggccag cgcggtccca ccgctgccgc cggagacagg gaagtccccg
      961 gcagaaaagg aaatgacctg ccccaccatg ccgtttttgg agaaatcgga aacgctgggg
     1021 acatcagttt tcttgttcca gaagaaggcg gaggcgggca gggacagggt acagaccagc
     1081 gccaacgcca gcaccggggc gagcatcctg cggaaagctg gggaatgatt caaaggaatg
     1141 acctccttac tttgttgatg tgcccttatt ttctgtgcta acaggaaaaa tatgcgggcc
     1201 cacatgccgg aaaagcccat ggaagatatt gagaaccagc aaaaggaagt gcaaaaggag
     1261 ctttgaggca gatcctccac atcatcttat ttagatacag aacgggcaga acgaagtgct
     1321 gccgtgatga atgaggggga tgttcttcca tcagaacaaa tcgccgggat cgggcgtgta
     1381 tgggacacgc ccctgtgtga gacggcaaga cctggcgcgc cgcgccgggc tcagcggtca
     1441 aaaccatccg aaagagggaa gataatgaca ggtgccttga cggggggcgg caaaggggat
     1501 acaatgaaaa aaactatgga tgtccacggg atgccgccgg gggcgcggca ccggcctgga
     1561 aaggaagttt catcatgaag actggattgg tgctggaggg cggagctctc cggacgattt
     1621 tttccagcgg ggtgtgcgac gccctgctgg acggcggcgt gatggtggac tacgtgatcg
     1681 gggtgtccgc cgggatcgcc tatggggtga gctatatctc ccgccagccc cgccgcaacc
     1741 tggagatcgt gacccggtac gccggggacc ggcggtatat ggggatgaac aacctggcgg
     1801 accggcacaa caggagctac ttcggcctgg agttcgtcta tgacaccatc cccaacgagc
     1861 ttgtcccctt tgattaccag acctttgccg cctatcccgg tcaggtggag gcggtggtga
     1921 ccaacctgag cacgggggag gcggagtata tgccagtccc gcgggaggat gacaaattcc
     1981 tgctgctcca ggccacctgc gccctgcctc tgatgttccc catctaccat ctgaatggac
     2041 agccctatct ggatggcggc gtggccgact ctatccccta tgaacgggcc ttccagcagg
     2101 ggtgcgaccg ggtggtggtg gtgctgagca agacccggag ctatgtgcgc cggccggaga
     2161 agctccagcc ggtgatcgag cggacctacc gggaatatcc caacttctgc cgggctatgg
     2221 cgcggcgggc agagcagtac aacacctgcc gggagcgcct gttccagctg gagcgggagg
     2281 gcagggtact ggtgctggcc ccggtgtcca cccacaaggt atcccgcatc gagcgggata
     2341 ccaaaaagct gaggatgctg tggggagatg ggtatcagca ggcggtggac cgaatggacg
     2401 agatccgggc tttcctcacg ccggacaggg cgtgacctcg tcctcctccg cagctggaca
     2461 gctgggctgg agggccagca gagccagggc gtcccccagc acagtgaaga aggcggccat
     2521 gcggccccgc tcgtccacgt cggcctccca ggccaggagg acggccagca ggacggccgc
     2581 cgccagaagg atgtaggaat ttcgggaaaa catatgggac aaaaaagacc accccccgca
     2641 gtccattcta tgtggacggc ggcggggcgg tctcttttta gtcagaaagt ggggcggtac
     2701 agtagaccgt cacacccccg gcagtgagat agagggcggc ctcccggctg ccatcggtgt
     2761 gctctgtgat accatacacc gcccagcctg cctcggcgcc ggggaaggac agggggtagg
     2821 cggtagtgcc gtcgga
//
LOCUS       DBMDSJ010000224         2827 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8666_length_2827_cov_1.559428, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000224 DBMDSJ010000000
VERSION     DBMDSJ010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8666_length_2827_cov_1.559428"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="ACZPAQ_06090"
     CDS             complement(<1..1104)
                     /locus_tag="ACZPAQ_06090"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR0903592.1"
                     /translation="MTVHTENEALFQRASRCLAGGVLSNFKKAKGVAPVYVKSAEGCR
                     MTDYDGNVYYDFSLSAGPAILGHSNEGLRRALREQLDQAYTNRDCLAAIEAAEKIQQV
                     VPCAELVRFAVSGVDAVLNAIRVARAYTGRPLYVKFKGQYNGGMDFVLGGRTDRGDLQ
                     ARDGVDPDDFYSAMCYTEGRAPHALDDCLLAEYNDLEGLAALFQQQGDQIACVIMEPV
                     TLNMTGCMPEPGFLEGLRELCTRYGVLMVFDETLTGFRLALGGAQEYFGVTPDLCTFA
                     KAIGGGFPVAAFAGRREVMQVIEDNRVLAVGTYNGHPLAAAAVLETIRQLEANDGEAF
                     RLIRRYTQMAKDGMEALARKHGVDMIVQGMPGAL"
     gene            complement(1248..1877)
                     /locus_tag="ACZPAQ_06095"
     CDS             complement(1248..1877)
                     /locus_tag="ACZPAQ_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0903593.1"
                     /translation="MPVKEKKTSRAEFAYDTLKRRILDNQLPPGAMLSEVALAQKLNM
                     SRTPIREALKMLMRDDLVEIRDGVGTFVKSATQKDIEDAYAVRQALEVLAARTAIGAF
                     SSEELDALEARFRSLQVRLEQGETVRVEEFADADWALHDQILQKSGNRYAQKATNDLR
                     AVLRRYQYLSVRLLSRVEESLTEHLAILDCIRRRDLARLTELLENHIQY"
     assembly_gap    2032..2041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2042..>2827)
                     /locus_tag="ACZPAQ_06100"
     CDS             complement(<2042..>2827)
                     /locus_tag="ACZPAQ_06100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR0903594.1"
                     /translation="PTLKANPGGAPGNLLAALSRYGRRTAFLGKVGEDAFGRLLLESL
                     RRAGVQTRGIRLTSSAFTTLAFVTLDAAGDRSFSFARKPGADTQLRWEEVDRSLIQRC
                     RLFHFGSLSLTDEPARSATRAAVAYARELGRTVTFDPNLRLPLWPSPAEARAQILWSL
                     CQADVVKLSDEEVDFLWGCSPQEGARRLLEECGVSLAMVTLGPRGCYLQNRRGSCALT
                     APQVSPVDTTGAGDIFGGSALHRLLDFRVPPEELDRGGLEEIAR"
BASE COUNT          494 a          885 c          974 g          464 t
ORIGIN      
        1 gagcgccccc ggcatcccct ggacgatcat gtccacgccg tgctttctgg ccagagcctc
       61 catgccatcc ttggccatct gggtgtaccg gcggatcagg cggaacgcct ctccgtcgtt
      121 ggcctccagc tgccggatag tctccagcac agccgcggcg gccaggggat ggccattgta
      181 ggtgcccacg gccaggacgc ggttgtcctc gatgacctgc atgacctccc gccggccggc
      241 gaaggcggcc acagggaagc cgccgccgat ggccttggca aaggtgcaca ggtcgggggt
      301 gacgccgaag tactcctggg cgcctcccag cgccaggcgg aagccggtga gggtctcgtc
      361 aaagaccatc agcacgccat accgggtaca cagctcccgc agtccctcca gaaagccggg
      421 ctcaggcatg cagccggtca tattcagggt cacaggctcc atgatgacgc aggcgatctg
      481 atccccctgc tgctggaaca gggcggccag cccctccaga tcgttgtatt ctgccaggag
      541 gcagtcatcc agggcgtggg gggcgcggcc ctcggtgtag cacatggcgg agtagaagtc
      601 atccgggtcc acgccgtccc gggcctggag atcgccgcgg tcggtgcggc cgcccagaac
      661 gaagtccatg cccccgttgt actgcccctt gaactttaca tacagggggc gtcctgtata
      721 agcccgggcc acccggatgg cgttgaggac tgcgtccacg ccggagacgg cgaagcggac
      781 cagctcggcg caggggacca cctgctggat cttttccgcg gcttcgatgg ccgccaggca
      841 gtcccggttc gtgtaggcct ggtccagctg ctccctgagg gcccggcgca gcccctcgtt
      901 gctgtggccc agaatggcgg gcccggcgga gagggagaag tcgtagtaca cattgccgtc
      961 atagtcggtc atgcggcagc cctcggcgct tttgacatag accggagcga cgcctttggc
     1021 cttcttgaaa ttggacagga caccgccggc cagacagcgg ctggcgcgct ggaacagagc
     1081 ttcattttcc gtatggacag tcatgtgggt ggcctccttg tggatcgttg tgctctgagt
     1141 ataacagatg catacatctt ggtcaataga aaaagtctaa attaaaaaag aaaattataa
     1201 gatgcataca ataagagatc ccccggcagt gtcgggggat ctccaaatca atattgaatg
     1261 tggttctcca gcagctctgt cagccgcgcc agatcccggc ggcggatgca gtccaggatg
     1321 gccaggtgct ccgtaaggct ctcctccacc cgggagagca ggcggaccga caggtactga
     1381 taccggcgca gcacggcccg caggtcgttg gtggccttct gggcatagcg gttgccgctc
     1441 ttttgcagga tctggtcgtg gagggcccag tccgcgtcgg caaactcctc cacgcggaca
     1501 gtctccccct gttccagccg tacctggagg gagcggaagc gggcctccag ggcgtccagc
     1561 tcctctgagg agaaagcgcc aatggcggtc cgggcggcca gcacctccag ggcctggcgc
     1621 accgcgtagg cgtcctcgat gtccttctgg gtggcagatt tgacgaaggt gcccacgccg
     1681 tcccggatct ccaccaggtc gtcccgcatc agcatcttca gggcctcccg gatgggggtg
     1741 cggctcatgt tcagcttctg ggccagggcc acctcgctga gcatggcgcc gggggggagc
     1801 tgattgtcca ggatacggcg cttcagggtg tcataggcga actctgcgcg gctggtcttt
     1861 ttctctttga cgggcaaagg gccacctcct tcaagacgga atgactgatt ataacacgga
     1921 aagggagagg gaacaagagg attcataggg agaggggggc gggctctccc atccgggcca
     1981 ccacctgggc cagctccggg atgctgggga tgccgccggg gtgctgagtt gnnnnnnnnn
     2041 ncgggcgatc tcctccaggc cgccccggtc cagctcctcc ggggggacgc ggaagtccag
     2101 caggcggtgg agggcgctcc cgccgaagat gtcgcccgcg ccggtggtat ccacagggga
     2161 gacctggggg gcggtcaggg cacagctccc cctccggttc tggaggtagc agccccgggg
     2221 acccagggtg accatggcca gggagacgcc gcactcctcc agcaggcggc gggccccctc
     2281 ctggggggag cagccccaga ggaagtccac ctcctcatcg ctgagcttga ccacatccgc
     2341 ctggcacagg ctccacagga tctgggcccg ggcctccgcg ggggagggcc agagggggag
     2401 acgcaggttg ggatcaaagg tgacggtccg gcccagctcc cgggcgtagg ccaccgcggc
     2461 ccgggtggcg gatcgggcgg gctcgtcagt gaggctgagg gagccgaagt ggaagagacg
     2521 acagcgctgg atgagggagc ggtccacctc ctcccaccgg agctgggtgt ccgcccccgg
     2581 cttgcgtgca aagctgaagg agcggtcccc cgcggcatcc agggtgacaa aggccagggt
     2641 ggtgaaggcg gaggaggtga ggcggatgcc ccgggtctgg actccagccc ggcgcaggga
     2701 ctccagaagg aggcggccga aggcatcctc tcccaccttg cccagaaagg cggtgcgccg
     2761 gccatatctg ctcagggcgg ccagaagatt gcccggcgcg cccccgggat tggccttgag
     2821 cgtggga
//
LOCUS       DBMDSJ010000225         2818 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8690_length_2818_cov_1.656607, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000225 DBMDSJ010000000
VERSION     DBMDSJ010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8690_length_2818_cov_1.656607"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /gene="feoB"
                     /locus_tag="ACZPAQ_06105"
     CDS             <1..1392
                     /gene="feoB"
                     /locus_tag="ACZPAQ_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HIR0903595.1"
                     /translation="ELVERALALPEPVQEQLDRVVAEYESASDLGDRETLVADSRYQY
                     IERVVAASVTKGQKPGELTLSEKVDKVVTGKYLALPLFLCAMLVMFVITFGPFGSWLS
                     NLVAAGIDTCSAALEETLLGAGVSQVLVSLICEGIIAGVGGVLSFLPQIALLFFFLSF
                     LEDIGYMSRAAFIMDRLLRRFGLSGKAFIPMLMGFGCSVPAIMGARTMENEKDRRMTI
                     LLVPFMSCSAKLPVYGLLSSAFFGPWAGLVVFGLYVIGMAAGILSGLLFKHTLFAGEP
                     APFVLELPPYRLPSLHNMLTHVWQKVKGFLIKAGTLILLMSILLWLLQSFDFSLHMVE
                     NEADSMLGALGSVIAPIFKPLGFGFWQAAVALLTGLIAKEMVVSSLSMFYAFPLTATG
                     AQVAAAMTGFTPLSAFSMLVFILLYVPCVAAVSTLAKEMNSTKWTLFSIGWQLGVAYV
                     ASLLVYQVGSLFL"
     gene            1414..2241
                     /locus_tag="ACZPAQ_06110"
     CDS             1414..2241
                     /locus_tag="ACZPAQ_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR0903596.1"
                     /translation="MIRLIALDLDGTLLDPAGRITQASKDAIARARAAGVRVVLNTGR
                     PIQEAVWFAGEAGCDALLSCLGGAAVVDGVRGETLRRWDMPEESGRRALELCLGREIE
                     LMIFAGEEIVVDPFSKESLSRTYPFPIFHSAAVVAEDPRAYLEERGLPLTKVHGDWNQ
                     ARYPLEELGALPGVQLTASNDHDFELVPDRVDKGRAMALIALLYGVPLGETAAVGDSD
                     NDLSMLRAAGLPIAMGNAPAAVREAAQRTAPSNQEEGVAWAISRCLEENEWERGEKG"
     gene            2238..>2818
                     /locus_tag="ACZPAQ_06115"
     CDS             2238..>2818
                     /locus_tag="ACZPAQ_06115"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HIR0903597.1"
                     /translation="MNFDELIQKEEEALLRDLRELVRIPSVSVPGTGRAPYGAECRRA
                     LDWFLSRASEMGFQTRDVDGHCGWCEYGEGEEMVAVLCHLDVVPAGDGWHYPPYDCTW
                     AGGRIYGRGVIDDKGPAAAALYALKAVRDSGAPLHRRVRLLVGCNEEKGSSCIRHYVE
                     AGGEIPVMGFTPDGMYPIINGEKGIAVVDFRRAL"
BASE COUNT          413 a          863 c         1015 g          527 t
ORIGIN      
        1 gagctggtgg agcgggcact ggccctgcct gagccggtgc aggagcagct ggaccgggtg
       61 gtggcggaat acgaaagcgc cagtgacctg ggtgaccggg agaccctggt ggcggacagc
      121 cgctaccagt acatcgagcg ggtggtagcc gcctcagtga ccaaggggca gaagccgggg
      181 gagctgaccc tcagcgagaa ggtggacaag gtggtgacgg ggaaatatct ggccctgccc
      241 ctgttcctgt gcgccatgct ggtgatgttc gtcatcacct tcggcccctt cggctcctgg
      301 ctgtccaatc tggtggcggc gggcatcgac acatgctccg ccgcgctgga ggagactctg
      361 ctgggggcgg gggtgtccca ggtactggtg agcctgatct gtgagggcat cattgccggc
      421 gtggggggcg tgctctcctt cctgccccag atcgctctgc tgttcttctt cctgtccttt
      481 ctggaggaca tcggctatat gtcccgggcg gccttcatca tggaccgcct gctgcggcgc
      541 ttcggcctga gcgggaaggc gttcattccc atgctcatgg gcttcggctg ctcggtgccc
      601 gccatcatgg gtgcccggac catggagaac gagaaggacc ggcggatgac catcctgctg
      661 gtgcccttta tgagctgctc cgccaagctg ccggtgtacg ggctgctctc ctcggctttc
      721 tttggaccct gggccgggct ggtggtcttc ggcctgtatg tcatcggcat ggcggcgggc
      781 atcctgtccg gcctgctgtt caagcatacc ctcttcgccg gggagcccgc cccctttgtg
      841 ctggagctgc ccccttaccg cctgccctcc ctgcacaaca tgctcaccca tgtgtggcag
      901 aaggtgaagg gcttcctcat caaggcgggc accctgatcc tgctgatgag catcctgctc
      961 tggctgctcc agtcctttga cttctccctg catatggtgg agaacgaggc ggacagcatg
     1021 ctgggtgccc tgggcagcgt catcgccccc attttcaagc ccctgggctt cggcttctgg
     1081 caggcggcgg tggctctgct caccggcctg atcgccaagg agatggtggt cagctccctg
     1141 tccatgttct acgctttccc gctcactgcc accggcgccc aggtggcggc ggccatgacg
     1201 gggttcaccc ccctgtctgc cttctctatg ctggtgttca tcctgctgta cgtcccctgc
     1261 gtggcggcgg tgtctacgct ggccaaggag atgaacagca cgaagtggac cctgttttcc
     1321 atcgggtggc agctgggggt ggcctatgta gcctctctgc tggtctacca ggtggggagc
     1381 ctgttcctat aattgctcaa ggagaagatg cccatgatac gactgatcgc gctggacctg
     1441 gacgggaccc tgctggaccc ggcgggccgg atcacccagg cgtccaagga tgccatcgcc
     1501 cgggcccggg cggcgggggt gcgggtggtg ctgaatacgg gacgcccgat ccaggaggcg
     1561 gtttggttcg ccggggaggc gggctgcgat gcgctgctct cctgcctggg gggcgccgcc
     1621 gtggtggacg gtgtccgggg ggagaccctg cgccgctggg acatgccgga ggagtcgggc
     1681 cgccgggcgc tggagctgtg cctgggccgg gagatcgagc tgatgatctt tgccggggag
     1741 gagatcgtgg tggacccctt ttccaaggaa tccctttccc ggacctaccc cttccccatc
     1801 ttccactccg ccgccgtggt ggcggaggac ccgcgggcct atctggagga gagggggctg
     1861 ccgctgacca aggtgcacgg ggactggaac caggcccgct accccctgga ggagctgggc
     1921 gcgctgcccg gggtgcagct gactgcctcc aatgaccacg actttgagct ggtgcccgac
     1981 cgggtggaca aggggcgggc catggccctg atcgcgctgc tgtacggcgt ccccctgggg
     2041 gagaccgccg ccgtggggga cagcgacaac gacctgtcta tgctccgggc ggcggggctg
     2101 cccatcgcca tgggcaatgc cccggcagcg gtgcgggagg cggcccagcg gaccgccccc
     2161 tccaaccagg aggagggagt agcctgggcc atctcccgct gtctggagga aaatgagtgg
     2221 gaaaggggag aaaagggatg aattttgatg aattgatcca aaaagaagag gaggccctgc
     2281 tccgggatct gagagagctg gtccgcatcc ccagcgtctc ggtccccggg acggggcggg
     2341 ccccctatgg ggcggagtgc cggcgggcgc tggactggtt cctgtccagg gcgtcggaga
     2401 tgggcttcca gacccgggac gtggacggcc actgcggctg gtgtgagtac ggggaggggg
     2461 aggagatggt ggctgtcctg tgccatctgg atgtggtccc cgccggagac gggtggcact
     2521 acccccccta tgactgtacc tgggcagggg ggcggatcta tggccggggg gtcatcgacg
     2581 acaaagggcc cgcggcggcg gccctgtacg ccctgaaagc ggtgcgggac agcggcgccc
     2641 ccctgcaccg ccgggtgcgc ctgctggtgg ggtgcaacga ggagaagggc tcctcctgta
     2701 tccgccacta tgtagaggcc ggcggagaga tcccagtgat gggcttcacc ccggacggca
     2761 tgtaccccat tatcaacggg gagaagggga tcgccgtggt ggacttccgt cgggcact
//
LOCUS       DBMDSJ010000226         2816 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8696_length_2816_cov_1.863352, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000226 DBMDSJ010000000
VERSION     DBMDSJ010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8696_length_2816_cov_1.863352"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="ACZPAQ_06120"
     CDS             <1..839
                     /locus_tag="ACZPAQ_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903598.1"
                     /translation="LLLVAGFHMGAAGAALATVAAQTVSVLVSLFLIRLRRRSLPFAF
                     SRRHLRFHRDIIGAELRLGTPVALQELLVGTSFLLIQAIVNSFGVAASAGVGVAEKVC
                     VFIMLVPSAYMQSMSSFVAQNMGAGNPIRAKKALKYGILTSVAAGLVMALLTFFHGDL
                     LSAIFSPDPEVVAASHSYLKAYAIDCMLTPFLFCFTGYYNGCEKTLFVMIQGIIGAFF
                     VRIPVAYLMSRMAGATLFQIGLSTPASSVVQILLCLFMFLFLERRSAGQSHASLKGSA
                     DP"
     gene            836..1459
                     /locus_tag="ACZPAQ_06125"
     CDS             836..1459
                     /locus_tag="ACZPAQ_06125"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR0903599.1"
                     /translation="MKIITVSREFGSGGRELGKRLSDCLGWPYYDREIISAVARRNAL
                     DEGYVEDALEQGDFRGVPLTIGRTFAYLPSMPHDSRQLLQEQRRVLRALAAHGDCIIV
                     GRNADLILAEHRPLKLFVYADLESRLRRCRERSSDDERFTPRELERKLRQVDQHRARH
                     HAMLSDLPWGRREGYHLCVNTTGLSLKTLAPLVAEYARSWFSQQGTP"
     gene            complement(1650..1859)
                     /locus_tag="ACZPAQ_06130"
     CDS             complement(1650..1859)
                     /locus_tag="ACZPAQ_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903600.1"
                     /translation="MTTMQATVLQVGNGQLLVQDQSTSQQVLVNTSIAGCFAVGNQVF
                     ITYNGVMTASIPPQISAMSICLIRS"
     gene            2052..2495
                     /locus_tag="ACZPAQ_06135"
     CDS             2052..2495
                     /locus_tag="ACZPAQ_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903601.1"
                     /translation="MSLYPNKIQVDGVPCGLILRGSANGTYLAVFEREMAELARIEQI
                     DWSRPAVVGESPLPAGYGFQVRDIRYAHATRSYTVLLQTAEQYLGDVAGFQAQLGELE
                     DTLQEKQALLQEQESMIAALEQAGSAAQLRSELEDAYQKGVELCG"
     gene            2488..>2816
                     /locus_tag="ACZPAQ_06140"
     CDS             2488..>2816
                     /locus_tag="ACZPAQ_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903602.1"
                     /translation="MADIVTLALEELEHRGGADALALAGRSVSGEADGTALIAEEQAI
                     PTWRPRSYLTEETPVGKPYRWNGQVYRLWQQHDATGQPGWSPDLAFSLWDICHTTDPA
                     RAKDYLPP"
BASE COUNT          463 a          944 c          886 g          523 t
ORIGIN      
        1 acctgctcct ggtggccggg ttccacatgg gggccgccgg ggccgccctg gccaccgtgg
       61 ccgcccagac cgtcagcgtg cttgtctccc tgttcctgat ccgcctgcgc cgccggtctc
      121 tgcccttcgc tttctcccgg cgacacctgc gcttccaccg ggacattatc ggcgctgagc
      181 tgcggctggg cacgccggtg gccctccagg agctgctggt ggggacctcc ttcctgctca
      241 tccaggccat cgtcaactcc ttcggcgtgg cggcctccgc cggcgtgggg gtggcggaaa
      301 aggtgtgcgt cttcattatg ctggtcccct ccgcctatat gcagtccatg tcctcctttg
      361 tggcccagaa catgggggcg ggcaacccga tccgggcgaa aaaggccctg aagtatggca
      421 tcctcacctc tgtggcggcc ggcctggtga tggccctgct caccttcttc cacggtgacc
      481 ttctctccgc catcttctcc cccgacccgg aggtcgtggc ggcctcccac agctatctga
      541 aggcctatgc catcgactgc atgctcactc cctttctctt ctgcttcacc ggctactaca
      601 atggctgtga aaagaccctg ttcgtcatga tccagggtat cataggtgcc ttcttcgtcc
      661 gcatccctgt agcctatctc atgagccgca tggcgggggc caccctcttc cagatcggtc
      721 tgagcactcc tgcctcctct gtggtccaga tcctgctctg tctgttcatg ttcctctttc
      781 tggagcgccg ctccgctgga cagagccatg cctctttgaa aggaagtgcc gatccatgaa
      841 gatcattacc gtcagccggg agttcggcag cggcggccgg gagctgggca agcggctgtc
      901 cgactgcctg ggctggccct actacgaccg cgagatcatc tccgccgttg cccggaggaa
      961 tgcactggac gagggctatg tggaggatgc gctggagcag ggcgactttc gtggcgtccc
     1021 actgaccatc ggccgcacct ttgcctatct cccctccatg ccccatgact cccggcagct
     1081 gcttcaggag cagcgccgcg tcctccgggc cctggccgcc cacggggact gcatcattgt
     1141 gggccgcaac gcggacctga ttctggcgga gcaccgcccc ctgaagctgt tcgtctacgc
     1201 cgatttggag tccaggctcc ggcggtgccg ggagcgcagc tccgacgacg agcgtttcac
     1261 tccccgagag ctggagcgga agctccggca ggtggaccag caccgggccc ggcaccacgc
     1321 catgctctcc gacctgccct gggggagacg ggaggggtat cacctgtgcg tgaacaccac
     1381 cggcctgtcc ctgaagacgc tggcccccct ggtggcggag tatgcccgct cctggttctc
     1441 ccagcagggg accccctgac ccgcgcccga aaaagagact gtcgaaaaag tctcaggctg
     1501 caagtgtttc ccccgacgca catgccgccg gggaaaacag gtcagaactt gattcgcgcc
     1561 tgcgggcgcg aactacgcga gtctttttcc acaagctgag cgccccggac gccttttcag
     1621 gcgtccgggg cgcttttccc atcaccgcgt cagctgcgga tcaggcagat gctcatcgcg
     1681 ctgatctggg gcgggatact ggcggtcatc acaccgttat aggtgatgaa cacctgattg
     1741 cctacggcaa aacagcccgc gatggacgta ttcacaagga cctgctggga tgtgctctgg
     1801 tcttggacca ggagctgacc gttccccacc tggaggacgg tggcttgcat cgttgtcaaa
     1861 aggatcacct cacatcaagt ggatgcaccc ccattatatg ccggagccgg gcgggtgtgc
     1921 ctcccggggc ccgccaggag atcctggggc aaggtgtacg gcaccgggca actgagaggg
     1981 ccgtcccgcc acacttaggg ggatatcccc ggccgcatgg cggcacaaca gttcagagag
     2041 gagagaacac aatgagcttg tatccaaaca agatccaggt ggacggcgtt ccctgcgggc
     2101 tgatcctgcg gggaagcgcc aacgggacct atctggccgt ctttgagcgg gagatggcgg
     2161 agctggcccg tatcgagcag atcgactggt cccgccccgc cgttgtgggg gaaagcccgc
     2221 tgcccgcagg gtatggcttt caggtccggg acatccggta cgcccacgct acccgcagct
     2281 acacggtgct tctccagaca gcggagcagt atctggggga tgtggccggc ttccaggccc
     2341 agttggggga gctggaggat actcttcagg agaagcaggc gctcctccaa gagcaggagt
     2401 cgatgatcgc ggcgcttgag caggcaggca gcgccgccca gctgcgctca gagctggagg
     2461 atgcctatca gaagggggtg gagctctgtg gctgacatcg tgaccctggc cctggaggag
     2521 ctggaacacc ggggcggggc agacgccctg gccctggcag ggcggtctgt gtccggcgag
     2581 gccgacggca ccgccctgat cgcggaggag caggccatcc ccacctggcg cccccgctcc
     2641 tatctcacgg aagagactcc ggtgggcaag ccctaccggt ggaacggaca ggtctaccgc
     2701 ctctggcagc agcacgacgc caccggtcag ccgggctgga gcccggactt ggctttctcc
     2761 ctgtgggaca tctgccacac cacggacccc gcccgggcca aggactacct cccgcc
//
LOCUS       DBMDSJ010000227         2809 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8723_length_2809_cov_1.804284, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000227 DBMDSJ010000000
VERSION     DBMDSJ010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8723_length_2809_cov_1.804284"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="ACZPAQ_06145"
     CDS             <1..243
                     /locus_tag="ACZPAQ_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR0903603.1"
                     /translation="HLCDCEAPIPTDEAALIHTGRAERLYPGEGAIPIAEIISRIPQV
                     VCGVEVPHLTRVEKIGYETHARRALEGAKACLGEQR"
     gene            261..1013
                     /locus_tag="ACZPAQ_06150"
     CDS             261..1013
                     /locus_tag="ACZPAQ_06150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR0903604.1"
                     /translation="MKRYEHKVVLITGAGDIAGATARRLLSEGGKVALTDFSREALDQ
                     EARMLKEEGWDEDRVMTIPGDVRSLEDCQAAAAEVKEKWGRIDVLVATAGILRHFPID
                     ELTEAQWQDVIDINLTGVYHSVKAVVPAMKEQNYGRIVLISSIGGRTGRPGVGVNYAA
                     AKAGINGMAMCLGYELGPWNITVNSVAPGPLKGKMFLSLSQDKVDKLSAGVRIPRLGE
                     LDDIAGAIAYLGSDDASWTTGEVLDVNGGLQY"
     gene            complement(1135..1794)
                     /gene="deoC"
                     /locus_tag="ACZPAQ_06155"
     CDS             complement(1135..1794)
                     /gene="deoC"
                     /locus_tag="ACZPAQ_06155"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR0903605.1"
                     /translation="MDYSELLRHVDHTLLTPTATWDQVKTVCDEGLTCRTASVCIPPR
                     YVKRAAAYVGNQLKICTVVGFPNGYSTPEVKVFETEDAIRGGADEIDMVADLGLVKSG
                     DWEGVLEEIKAVKASCKGRILKVIVEACLLTCEEKQAMCRIVSMSGADFIKTSTGFST
                     GGATYEDVKLFREHCSPDVRIKAAGGIRTLEEAQTYLELGADRIGSSGLVALVQAQSN
                     G"
     gene            complement(1998..>2809)
                     /gene="metK"
                     /locus_tag="ACZPAQ_06160"
     CDS             complement(1998..>2809)
                     /gene="metK"
                     /locus_tag="ACZPAQ_06160"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR0903606.1"
                     /translation="GAGDQGMMFGYACDETPELMPLPISLAQRLARRLTQVRKEGLVD
                     YLRPDGKTQVTVEYDGENRPLRVDAVVVSTQHGPEASLDQIRQDMIQLVIKPIIPAEL
                     LDDQTKIYVNPTGRFVIGGPQGDSGLTGRKIIVDTYGGSAPHGGGAFSGKDPTKVDRS
                     AAYAARWVAKNVVAAGLARRCQVQLAYAIGVARPVSVRVDTFGTGTVPDEQLSDAVEQ
                     VFDLRPAAIIRDLGLRRPIYRQLAAYGHFGRDDLDLSWEKTDRAEALKAAL"
BASE COUNT          538 a          846 c          911 g          514 t
ORIGIN      
        1 cacctgtgcg actgtgaggc cccgatcccc acggatgagg cggcactgat ccacaccggc
       61 cgggcggaac gcctgtatcc cggggagggt gcaatcccca tcgcggagat catttccagg
      121 atcccgcagg tggtgtgcgg tgtggaagtg ccccacctga caagagtaga aaagataggc
      181 tatgagaccc atgccagacg ggccctggaa ggggcaaagg catgtctagg ggagcaacga
      241 taagactgga gggatatgca atgaagcgat atgaacataa agttgtgctg atcactggcg
      301 cgggggatat cgccggcgcc actgcccggc ggctgctgtc tgagggcgga aaagtggcgc
      361 tgaccgattt ttcccgggag gcactggacc aggaggccag gatgctgaaa gaggaaggct
      421 gggatgagga ccgggtgatg accatcccgg gcgacgtccg aagcttggag gactgccagg
      481 ccgccgcagc cgaagtaaag gaaaagtggg gcaggatcga cgtcttggta gccacggcag
      541 gtatcctccg tcactttccc atcgatgagc tgacagaagc ccagtggcag gatgtgatcg
      601 acattaactt gacaggggtc tatcattcgg tgaaagcggt ggtccccgcc atgaaggagc
      661 agaactacgg ccggatcgta ctgatctcct ccattggggg ccgcacgggc cgcccggggg
      721 tgggggtgaa ttacgctgcc gcaaaggctg ggatcaacgg catggccatg tgcctgggat
      781 atgagctggg cccctggaac attaccgtga acagcgtagc gcctgggccg ctgaagggga
      841 aaatgttcct ctctctctct caggataagg tggataagct ctctgcgggt gtccgcatcc
      901 cgagactggg agagctggat gacatcgccg gcgccatcgc atacctgggc agcgatgacg
      961 catcctggac cactggtgag gtgctggatg tgaacggagg cctgcaatac tgaccgatcc
     1021 agaaccgtgg gcccaacgag aggtcactcc tgcgggatac gcctgcagaa cgcaaaaaga
     1081 tccccgtcca ttttcacaat ggacggggat ctttcggtgt gaaaagggct gggctcagcc
     1141 gttgctctga gcctggacca gggcaaccag accgctgctg ccgatacggt cggcccccag
     1201 ttccaggtag gtctgggcct cctccagggt gcggatgccg ccggccgcct tgatcctcac
     1261 atcggggctg cagtgctccc ggaacagctt cacatcctca taggtagcgc cgccggtaga
     1321 gaagccagtg gaggtcttga taaagtctgc tccggacatg gagacgatac ggcacatggc
     1381 ctgcttctcc tcacaggtca gcaggcaggc ctccacgatg accttcagga tacgtccctt
     1441 acaggatgct ttgaccgcct tgatctcctc cagcaccccc tcccagtcgc cgctcttcac
     1501 caggcccaga tcagccacca tgtcgatctc gtccgctccc ccgcggatgg cgtcctcggt
     1561 ctcgaacacc ttgacctcag gagtggagta gccgttgggg aaacccacca cggtgcatat
     1621 cttcagctga ttgcccacat aggcggcggc ccgcttcaca tagcggggcg ggatgcagac
     1681 ggaggcagtg cggcaggtga ggccctcgtc acagaccgtc ttgacctggt cccaggtggc
     1741 ggtgggggtc agaagtgtgt gatccacgtg gcgaagcagt tcgctgtaat ccatattcta
     1801 tgatctcctt cctgtatggg gcgcagagat acccattttc ggctgttttc tctctatttt
     1861 accctatggc gcggcgattg tccagccccg gggccggtca ttcagaaaga aggggccgga
     1921 ggatctcctc cggcccttat ttgcgcctgt acagtctatg aggcgccaac tgaaaagatt
     1981 cctggccgct gacgccgtta cagagccgcc ttcagtgcct ctgcccggtc ggtcttctcc
     2041 caggacaggt ccaggtcgtc ccgcccaaag tgcccatagg ccgccagctg gcggtagatg
     2101 gggcggcgca gccccagatc ccggatgatg gcagccggac gcaggtcaaa gacttgttcc
     2161 actgcgtcgg agagctgttc gtccggcacc gttccggtgc cgaaggtatc cacgcggacg
     2221 gagacggggc gggccacacc aatggcataa gccagctgga cctggcagcg ccgggccagc
     2281 ccggcggcca ccacgttctt cgccacccag cgggcggcat aggcggccga ccggtccacc
     2341 ttagtggggt ccttgccgga gaaagcgccg cctccgtggg gagcactgcc gccgtaggtg
     2401 tccacgatga tcttccgccc ggtgaggccg gagtctccct gaggaccgcc gatgacgaac
     2461 cggccggtgg ggttgacgta gatcttggtc tgatcgtcca gcagctcggc agggatgata
     2521 ggcttgatga ccagctggat catatcctgc cggatctggt ccagggacgc ctccggcccg
     2581 tgctgggtgg agaccaccac cgcgtccacc cgcagggggc ggttctctcc gtcatactcc
     2641 accgtgactt gggtcttgcc atcagggcgc aggtagtcca ccagtccctc cttgcggacc
     2701 tgggtcaggc ggcgggccag ccgctgggcc agagagatgg gcagcggcat cagctcgggg
     2761 gtctcgtcgc aggcgtagcc gaacatcatg ccctggtccc cagccccgt
//
LOCUS       DBMDSJ010000228         2807 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8727_length_2807_cov_2.024390, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000228 DBMDSJ010000000
VERSION     DBMDSJ010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8727_length_2807_cov_2.024390"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="ACZPAQ_06165"
     CDS             <1..598
                     /locus_tag="ACZPAQ_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP synthase beta subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR0903607.1"
                     /translation="SAAQKEVPGRRGYPGYLYTNLATIYERAGRQLGKEGSITLIPIL
                     TMPEDDKTHPIPDLTGYITEGQIILSRELFRRGINPPVDVLPSLSRLKDKGIGVGRTR
                     EDHSGTMNQLFAAYATGKENKELMSILGEAALSPTDLLYAKFADEFEKRYVSQGTEEN
                     RSIQETLDLGWELLSILPTAELKRIKPELIEKYLPQKG"
     gene            611..1324
                     /locus_tag="ACZPAQ_06170"
     CDS             611..1324
                     /locus_tag="ACZPAQ_06170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR0903608.1"
                     /translation="MPSTTINPTRMELTRLKGRLRTAQRGHKLLKDKRDELMKQFMEM
                     VRENRALRRRVEEQLMRAQSSFTVAAAVMSPEMLEQSLLYPKQSVELDMTFQNIMSVD
                     VPQYHFRTHSQDPGEIYPYGFANTSGELDDAVSAMSQVFQDMLKLAEIEKTSQLLAEE
                     IEKTRRRVNALEYVKIPEMQENIKYITMKLDENERANTIRLMKVKELLLRDAIEERRE
                     ADAKAVEAFAGYPEGPAGV"
     gene            complement(1396..2304)
                     /locus_tag="ACZPAQ_06175"
     CDS             complement(1396..2304)
                     /locus_tag="ACZPAQ_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0903609.1"
                     /translation="MTLNQLYYFRTAAGQLNFHRTAEQLMISQPSLSAAIASLERELG
                     VPLFLRKGRHLELSKYGLLYFKEVDALLGRLDSVNATLKRAARPGQGHIDVGYIAPLS
                     PKFMPETVRAFLQQPGYEQVTFGFRELPTRELLDGLKTFLYDVVFCIYEEGEPEVEFV
                     PLLDQELVAIVPPDHPLAAEDQILLSQLAPYPFITYMPHVQIYRDIMAYISQSGWTPQ
                     VFCNATGEASISSLVASGFGVSIVAKTDVLDNSQVKLLHLKDRLYKRTIYMAYRRDEE
                     QPPSMQAFLRFVLQRARAQSDSQRHS"
     gene            2549..>2807
                     /locus_tag="ACZPAQ_06180"
     CDS             2549..>2807
                     /locus_tag="ACZPAQ_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012334.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR0903610.1"
                     /translation="MERYQDKVVLITGAGDVAEAAAKRLLCEGAKVAFSDFSQEALDA
                     AISGLKAEGWDGERLMGIKCDVRKYEECEAAANAVLARWGQI"
BASE COUNT          634 a          782 c          890 g          501 t
ORIGIN      
        1 ctccgccgcc cagaaggagg tcccgggccg ccggggctac cccggctatc tgtacaccaa
       61 cctggccacc atctacgagc gggcgggccg ccagctgggc aaggagggct ctatcaccct
      121 catccccatc ctgaccatgc cggaggatga caagacccac cccatccctg atctgacggg
      181 gtatatcacc gagggacaga tcatcctctc ccgggagctg ttccgccggg gcatcaatcc
      241 gccggtggac gtgctgccct ccctgtcccg cctgaaggac aagggcatcg gcgtgggcag
      301 gacccgggag gaccactcgg gcaccatgaa ccagctcttc gccgcctatg ccaccggtaa
      361 ggagaacaag gagctgatgt ccatcctggg cgaggccgcc ctcagcccca ccgacctgct
      421 gtatgccaag ttcgcggacg agtttgaaaa gcgctatgtt tcccagggaa cagaggagaa
      481 ccgctccatc caggagacac tggacctggg ctgggagctg ctgtctatcc tgcccaccgc
      541 ggagctcaag cgcatcaagc cggagctgat cgagaagtat ctgccccaaa agggctaagg
      601 ggggaccaga atgccttcca cgaccataaa tcccacccgt atggagctga cccgcctgaa
      661 gggccgcctg cgcaccgccc agcggggcca caagctgctc aaggacaagc gggatgagct
      721 gatgaagcag ttcatggaga tggtgcggga gaaccgggcc ctccgccgcc gggtggagga
      781 gcagctgatg cgcgcccaga gctccttcac ggtggctgcc gctgtcatgt ccccggagat
      841 gctggagcag tccctgctct atcccaagca gagcgtagaa ctggacatga cttttcagaa
      901 tatcatgtcg gtggatgtgc ctcagtacca cttcaggacc cacagccagg acccggggga
      961 gatctacccc tacggcttcg ccaacacctc aggagagctg gatgacgcgg tctctgccat
     1021 gtcccaggtg ttccaggata tgctgaagct ggccgagatc gaaaagacct cccagctgct
     1081 ggcggaggag atcgaaaaga cccgccgccg agtcaatgcc ctggagtatg taaagatccc
     1141 ggagatgcag gaaaatatca aatatatcac catgaagctg gacgaaaacg agcgggccaa
     1201 caccatacgc ctgatgaagg tgaaggagct cctgctcaga gacgccattg aggagcggag
     1261 agaggccgac gccaaggcgg tggaggcctt cgccggatac ccggagggac cggcaggcgt
     1321 ttgacgccca gcaggatctg catgaaagag cccggatggg accatagggt cccatccggg
     1381 cttttcgcag cccgttcagc tgtgccgctg agagtcggac tgcgcccggg cccgctggag
     1441 gacgaaacgc aggaaggcct gcatagaggg gggctgctcc tcatcccggc gataggccat
     1501 atagatggtg cgcttgtaga ggcggtcctt caggtgaagg agcttcactt ggctgttgtc
     1561 cagcacatcc gttttggcca cgatggagac tccgaagccg ctggccacca gagaggagat
     1621 gctggcctct ccggtggcat tgcagaatac ctgaggcgtc cagccgctct gggagatgta
     1681 cgccatgata tcccggtaga tctggacatg gggcatatag gtgataaagg ggtagggggc
     1741 gagctgactg agcaggatct ggtcctccgc agccagcgga tggtccggcg gcacgatggc
     1801 caccagctcc tggtccagca gggggacgaa ctccacctcc ggctcgccct cctcgtagat
     1861 acagaagacc acgtcataga ggaaggtctt cagcccgtcc agcagctccc gggtgggcag
     1921 ctcccgaaag ccgaaggtga cctgctcata gcctggctgc tggagaaagg cccgcaccgt
     1981 ctcgggcatg aatttggggg agaggggggc gatgtagccc acgtcgatgt ggccctgccc
     2041 cgggcgggcg gcccgcttca gggtggcgtt cacagagtcc aggcggccca ggagggcgtc
     2101 cacctcctta aaatagagca ggccgtattt gctcagttcc aggtggcgtc cctttcggag
     2161 aaagaggggg actcccagct cccgttccag gctggcgatg gcggcgctga gggagggctg
     2221 ggagatcatc agctgttccg cggtccggtg gaagttcagc tgtccggctg ctgtcctaaa
     2281 ataatagagc tgattcaatg tcaaattgct gcacctcccc aaaaatgcgg aaaaatatcc
     2341 tatatatttc cctatatttt attaaaaatt gagaagtttg tcaagaaata aacaaatatt
     2401 gataaaatta aatctatcaa aaaggcaatg aattggtatt ttactttttg acggcttggc
     2461 tggataatag agacaggaaa aggaaaagaa aacccaatta gatttgtgat aatatccaaa
     2521 caaagagctt tagaaatgag ggatcgatat ggagcgttat caggataaag tggtgctgat
     2581 caccggtgcg ggtgacgtgg ccgaggcggc tgctaagcgg ctgctgtgtg agggagcaaa
     2641 ggtggctttc tctgacttct cccaggaggc gctggacgca gccatctccg ggctgaaggc
     2701 cgaggggtgg gacggggagc gccttatggg cataaagtgc gacgtgcgga agtatgagga
     2761 gtgcgaggcg gcggccaatg ccgtcctggc caggtggggg cagatcg
//
LOCUS       DBMDSJ010000229         2783 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8807_length_2783_cov_1.755406, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000229 DBMDSJ010000000
VERSION     DBMDSJ010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8807_length_2783_cov_1.755406"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="ACZPAQ_06185"
     CDS             complement(<1..219)
                     /locus_tag="ACZPAQ_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903611.1"
                     /translation="MGGKRRGSGLRALRRGAALGLALTALWIFGLTADLSGAWDALGR
                     LGQTPSFVLGALSQELEGAPAGDGLSPDL"
     gene            complement(347..1402)
                     /locus_tag="ACZPAQ_06190"
     CDS             complement(347..1402)
                     /locus_tag="ACZPAQ_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016999.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HIR0903612.1"
                     /translation="MGWTLLTVAAAAGGGLLLMRLRVPGGMLVGAILGAVLVNLTTGQ
                     AYIWPQTRVVAQVLTGAYIGCLVTTKDLRHLPRVIRPYLAVMSTFLALNLAVGFFIWR
                     VTDLDLLTSLFCAAPGGMTDTPLIALDMGADASMVAVMQFVRMVFGMACLPSIIVLAD
                     RKIEGDQMVPEEGQGTHSLERKKAHVSLRKFLPTFAVALAAGILGKLSGVPAGTLSAS
                     LIAVIVLKFAGSAPPMPMWVRRLAQVVSGCCIGAGITRDQIFQLRQLILPALVLIAGY
                     ILVCVGMGRAISRAFRMDLKESMLSLSPAGATEMALIAADLGVHSTNLVVLQICRLLG
                     VTVIFPQIFALLSNLIG"
     gene            complement(1407..2366)
                     /locus_tag="ACZPAQ_06195"
     CDS             complement(1407..2366)
                     /locus_tag="ACZPAQ_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR0903613.1"
                     /translation="MLGEFLNAVSAVVVLLMLMAVGYFMGTKGWMKAEEKKFLSKYII
                     NIAVPCNCINGLLNNLDQSMLAQAGLMLVSAIIGVVITILLGMGLATLLRLPKNRWGV
                     FAAMVGVSNTLFVGLPLSTQLFGDVCVPYVMIYYLANTIFTQSVILMLVERSGTASHS
                     RGIKGFLKDVFTKPPILTVIASVLMLIVGFRPPEVFMSFAKYISGSVSPLALIYCGFI
                     IYELGLSNLRPSQLRQMKGLPTMLAARLVISPLICAGMCRLFGITGLAYQVFVVESAL
                     PVVSQVTVMAGDFGADDKYAATGATLSTLACFITIPVLMVLMG"
     gene            complement(2450..>2783)
                     /locus_tag="ACZPAQ_06200"
     CDS             complement(2450..>2783)
                     /locus_tag="ACZPAQ_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903614.1"
                     /translation="PLAAALEAVFNGERPPEPEGERPRGEGEGRRRRRRGGRSEGASG
                     GESRSQGEGQQRSRREWERRPEKPRDKEPGTAEAEGVQEGGERKPSRRRRRRPRGNRP
                     APGPEGNG"
BASE COUNT          542 a          957 c          819 g          465 t
ORIGIN      
        1 caggtcggga gagagcccgt cccccgctgg ggccccctcc agttcctgac tcagcgcgcc
       61 cagaacgaag gagggggtct gtcccagccg ccccagggcg tcccaggccc cggacagatc
      121 tgccgtcaat ccaaagatcc acagtgccgt cagcgccagc cccagggccg ctccccggcg
      181 cagagcgcgc agtccgctcc ccctcctctt accgcccatg cgatcactcc ttttgtaagc
      241 aagcctgaag catcctatgc ccagatgggg agatttatga cgggcctgtc cttttgctcc
      301 gcggaaaaac tgtgcccgcc tccataggag gcgggcacag catattttat cctatcaaat
      361 tggagagcag ggcgaagatc tggggaaaga tgaccgtcac tccaagcagg cggcagatct
      421 ggagcaccac cagattggtg ctgtggaccc ccaggtcggc ggcaatgagg gccatctccg
      481 tggctcctgc gggggagagg gagagcatgg actccttcaa gtccatccga aacgccctgg
      541 agatcgcccg gcccatcccc acacagacca ggatataccc ggcgatcagc accagggcgg
      601 gcaggatcag ctggcgcagc tggaagatct gatcccgggt gattccggca ccgatgcagc
      661 agcccgacac cacctgggcc agccgccgca cccacatggg cattgggggc gcgctgcccg
      721 caaatttcag cacgatcact gcgatcagag aggcactgag ggtacccgcc ggcacaccgg
      781 acagcttgcc caggatgccc gctgccagcg ccacagcgaa ggtaggaaga aacttccgca
      841 ggctgacatg cgcctttttt ctttccaggg agtgcgttcc ctgaccctcc tccggcacca
      901 tctggtcccc ctctatcttc cggtcggcca ggacaatgat ggagggcaga caggccatgc
      961 cgaataccat ccgcacgaac tgcatcaccg ccaccatgga ggcgtccgcc cccatgtcca
     1021 gggcgatgag cggggtgtcc gtcataccgc cgggagcggc acaaaacagg gaagtgagca
     1081 gatccaggtc tgttacccgc cagatgaaaa atcccaccgc cagattcagg gcgagaaagg
     1141 tggacatcac cgccagatag ggccgaatga cccggggcag atgtctcagg tccttcgtag
     1201 tcaccagaca gccgatgtag gccccggtca gcacttgggc caccacccgg gtctgaggcc
     1261 agatgtacgc ctgtcctgta gtcaggttca ccagtaccgc gcccaggatg gcacctacca
     1321 gcatcccgcc ggggacccgc agcctcatca gcagcaggcc gccccccgcc gcggcggcca
     1381 cggtcaaaag tgtccatccc atccactcac cccatcagca ccatcagcac agggatggtg
     1441 ataaagcagg ccagggtgga cagggtggca ccggtggcgg catacttgtc gtccgccccg
     1501 aagtcccccg ccattacggt cacctgactg accaccggca gggcgctctc cactacaaag
     1561 acctgatagg ccaggccggt aatgccgaac aggcggcaca tgcctgcaca gatcagcggt
     1621 gagatcacca gccgggccgc cagcatggtt ggcaggccct tcatctgccg cagctggctg
     1681 ggccgcaggt tgctcagccc cagttcatag atgatgaagc cgcagtagat cagcgccagg
     1741 ggggagacgg agccgctgat gtacttggca aagctcatga acacctcggg aggacggaag
     1801 cccactatca gcatcagcac cgaggcgatc acagtgagga tggggggttt ggtgaacaca
     1861 tccttcaaaa agcccttgat tccccggctg tgggaggcgg tgcccgaccg ctccaccagc
     1921 atcagaatta ccgactgggt gaagatggtg ttggccagat agtagatcat gacgtagggg
     1981 acgcacacgt ccccgaacag ctgggttgac aggggcagcc cgacgaacag ggtgttggac
     2041 acccccacca tggcggcaaa gacgccccac cggtttttgg gcaggcgcag cagggtggcc
     2101 agccccatgc ccagcaggat ggtgatgacc actccaatga tggcggatac cagcatcagc
     2161 cccgcctggg ccagcatgct ctggtccaga ttgttcagca ggccgttgat gcagttgcag
     2221 gggaccgcga tgttgatgat gtacttgctc aggaactttt tctcctccgc tttcatccag
     2281 cccttggtcc ccataaaata gcccaccgcc ataagcatca gcaggaccac cacggcggag
     2341 accgcattta aaaattctcc cagcatccgg gacacctcac tttatcgatc caagatgggt
     2401 cagccccgga catcctctct cctatgatgt ccggggcttt ttttctcttt cagccattcc
     2461 cctctggtcc cggagcgggc cggttccctc tgggccggcg gcgccggcgg cggctgggct
     2521 tccgctctcc gccctcctgg acgccctccg cctccgctgt cccgggctcc ttgtccctcg
     2581 gcttctccgg ccggcgctcc cactctcttc tggagcgctg ctggccctca ccctgggaac
     2641 ggctctcccc gccggacgca ccctcgctcc ggccgcctct gcggcggcgg cggcgcccct
     2701 cgccctcgcc tctgggacgc tctccctccg gctccggggg acgctcccca ttgaacaccg
     2761 cctccagggc ggcggccagt ggg
//
LOCUS       DBMDSJ010000230         2779 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8817_length_2779_cov_1.657954, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000230 DBMDSJ010000000
VERSION     DBMDSJ010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8817_length_2779_cov_1.657954"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="ACZPAQ_06205"
     CDS             <1..368
                     /locus_tag="ACZPAQ_06205"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903615.1"
                     /translation="VYENIVLPIQLDGGKVDQSYVNQVIEALGLEQKLQNLPSQLSGG
                     QQQRVAIARALVTKPAIILADEPTGNLDSKTSQDVLSLMKVTGQKFAQTMVMITHNEE
                     IAQMADRIVRIEDGRIVKR"
     gene            382..>2779
                     /locus_tag="ACZPAQ_06210"
     CDS             382..>2779
                     /locus_tag="ACZPAQ_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0903616.1"
                     /translation="MNVSNGKCIRDLSRKSLRANRTRNLIAVLAIALTTVLFTSLFTI
                     AMSINDGIQQNNFRQVGGFSHGGFKYLTEEQFHELKDDPLIDQWGLRRFVGMPSEVPF
                     NKSHVEVGYSDANEAHWMYCDPVEGRLPQEGTDEAATDTHVLELMGVTPEIGAKFTLT
                     FDVDGHETTQTFTLCGWWEYDEAIVANHILIPESRADAILTETGVVPGQTTDGMTGTY
                     NMDVMLKNGARNIAGDLDQILENHGYQAESSAQPGYIHTGVNWGYTGAQLSGSMDPAT
                     FIAICAVLLLIIFTGYLIIYNVFQISVTNDIRFYGLLKTIGTTPRQLRRIIRHQALAL
                     SLAGIPLGLVLGWLIGGQLTPVIVRQVNGVSNVVSMDPIIFTASTLFSLVTVLLSCRK
                     PGKMAARVSPIEAVRYTEGGNLKRKAKRRAKGVSLFSMAWANLGRSRGKTAVTVLSLS
                     LAVVLLTVTVTFTQGFDMDKYVSNFTASDFIVADAGQFQTGGDAYNDEMDVTEDVIAA
                     IDAQGGITGGGLVYGKTSAALEFVTEEYYRSVWSRWNTPEQLDSMVHFKDRNEEGLLA
                     DRVQLYGMEQFPLDHLTVLAGDLSKLNEPGGRYVAAVYSDDDYGQPEMDSHWARLGDT
                     VTLRYVEEYEYYNPDTGEIYGPWENVPEGANWVDRAVKYRDIEYEVAALVTVPMALSY
                     RYYGADEFIMNDQTFIQDTGTDSVMYYAFDTTDEANPAMESFLADYTENVNPQFDYES
                     KSTYAAEFEGMRSMFLLLGGALSGIVGLVGVLNFFNAILTGIITRKRELAVLQSIGMT
                     G"
BASE COUNT          550 a          878 c          849 g          502 t
ORIGIN      
        1 gcgtgtatga aaacatcgtc ctgcccatcc agctggacgg cggcaaggtg gaccagtctt
       61 atgtaaatca ggtcatcgag gccctggggc tggagcagaa gctccagaac ctgcccagcc
      121 agctctctgg gggacagcag cagcgcgtgg ccatcgcccg ggctctggtc accaagcccg
      181 ctatcatcct ggccgacgag cccacaggca acctggactc caaaaccagt caggacgtgc
      241 tctccctgat gaaggtcacc gggcagaagt tcgcccagac catggtgatg atcacccaca
      301 acgaggagat cgcccagatg gccgaccgca tcgtccgcat tgaggacggc aggatcgtca
      361 agcgctgagg gggtggattc catgaacgtc tccaacggca agtgtatccg agacctctcc
      421 cgtaagagcc tgcgagccaa ccgcacccgg aacctgatcg cggtgctggc catcgccctg
      481 accacggtgc tcttcacctc cctgttcacc atcgccatgt ccatcaacga cggcatccag
      541 cagaacaact tccgccaggt gggcggcttc tcccacggcg ggttcaagta cctgacagag
      601 gagcagtttc acgaattgaa ggacgacccc ctcatcgacc agtggggtct gcggcgcttt
      661 gtgggcatgc ccagtgaggt gcccttcaac aaaagccatg tggaggtggg ctactccgat
      721 gccaacgagg cccactggat gtactgcgac ccggttgagg ggcgcctgcc ccaggagggc
      781 accgatgagg ccgccacgga cacccatgtg ctggagctga tgggggtcac gccggagatc
      841 ggcgccaagt tcacgctcac ctttgacgtg gacggccatg agaccaccca gactttcacc
      901 ctgtgcggct ggtgggagta cgacgaggcc attgtggcca accacattct gatcccggag
      961 agccgggcgg acgccatcct gacggagacc ggcgtggtgc ccgggcagac cacggacggc
     1021 atgaccggga cctataacat ggatgtgatg ctgaaaaatg gggcccgaaa tattgcaggc
     1081 gacctggacc agatcctgga aaaccatggc tatcaggccg agagctccgc gcagccgggc
     1141 tatatccata ccggcgtcaa ctggggctac accggcgctc agctgtccgg cagcatggac
     1201 cccgccacct tcatcgccat ctgcgcggtg ctgctgctca tcatcttcac cggctacctc
     1261 atcatttaca atgtgttcca gatctccgtc accaacgaca tccgcttcta tggcctgctg
     1321 aagaccatcg gcaccacccc ccggcagctg cggcgcatca tccggcatca ggccctggcc
     1381 ctgtccctgg cgggcatccc cctggggctg gtgctgggct ggctcatcgg cgggcagctg
     1441 acgccggtga tcgtccggca ggtgaacggg gtgtccaatg tggtgtcaat ggacccgatc
     1501 atttttacgg cctccaccct gttttccctg gtgaccgttc ttctctcctg ccgcaagccg
     1561 ggaaaaatgg ccgcccgggt ctcccccatc gaggcggtgc gctacaccga gggcgggaac
     1621 ctgaaacgca aagccaagcg cagagccaag ggcgtctccc tcttctccat ggcctgggcc
     1681 aacctgggcc gcagccgggg caagacggcg gtcaccgtcc tctctctgtc cctggcggtg
     1741 gtgctgctca ccgtgacggt caccttcacc cagggctttg acatggacaa gtatgtctcc
     1801 aacttcactg ccagcgactt catcgtggcc gacgcgggcc agtttcagac cggcggcgac
     1861 gcctataacg acgagatgga cgtgacggag gacgtgatcg ccgccatcga cgcccagggc
     1921 ggcatcaccg gcggcggctt ggtctacgga aagacctccg ctgcgctgga gttcgtcacc
     1981 gaggagtact accgctccgt ctggagccgc tggaacaccc ccgagcagct ggacagcatg
     2041 gtccacttca aggaccgcaa tgaggagggc ctgctggccg accgggtgca gctctacggc
     2101 atggagcagt tccctctgga ccacctgaca gtgctggcgg gggacctgtc caagctcaac
     2161 gagcctggcg ggcggtatgt ggccgctgtg tatagtgacg acgactatgg tcagcccgag
     2221 atggactccc actgggcccg gctgggggac acagtcaccc tgcgctatgt ggaagagtac
     2281 gagtattata accctgacac tggcgagata tatggcccct gggagaatgt gcccgagggc
     2341 gccaactggg tggatcgggc cgtaaagtac cgagatatcg agtacgaggt ggccgccttg
     2401 gtgacggtgc ccatggccct gagctaccgc tattacgggg cggacgagtt tattatgaac
     2461 gaccagacct ttatccagga cacgggcaca gacagcgtca tgtactatgc cttcgacact
     2521 acggatgagg ccaaccccgc tatggagtcc ttcctggcgg actacacgga gaatgtgaat
     2581 cctcagttcg actatgagag caagtctacc tatgccgcgg agttcgaggg gatgcgctcc
     2641 atgttcctac tgctaggcgg ggccctcagc ggcatcgttg gactggtggg ggtgctgaac
     2701 tttttcaatg cgatcctcac cggcatcatc acccgaaagc gggagttggc cgtcctccag
     2761 tccatcggca tgacaggtg
//
LOCUS       DBMDSJ010000231         2772 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8847_length_2772_cov_1.054287, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000231 DBMDSJ010000000
VERSION     DBMDSJ010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8847_length_2772_cov_1.054287"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /gene="cbiD"
                     /locus_tag="ACZPAQ_06215"
     CDS             <1..860
                     /gene="cbiD"
                     /locus_tag="ACZPAQ_06215"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR0903617.1"
                     /translation="ALLGGLGVGRVTRPGLDQPVGEAAINSVPRRMIARAAEEEAAAL
                     GYAGGLEITISVPGGEEAARRTFNPQLGVEGGISILGTSGIVEPMSAQALVETIALEL
                     RQTAAEGTDRVVLTPGNYGADFLAESGLITSDIRIVKCSNFIGEALDRAAEAGIRQLI
                     LVGHLGKLVKVAGGVMNTHSRWADCRMEILCAHAALCGAGQETARALMGSATTDAGLD
                     ILAEAGLLDRTMDSLLSALQAHLERRAGTSCRCGGVLFTNTRGLLGMTGTAKEILNIW
                     RAKEEHFTP"
     gene            827..1519
                     /gene="cobI"
                     /locus_tag="ACZPAQ_06220"
     CDS             827..1519
                     /gene="cobI"
                     /locus_tag="ACZPAQ_06220"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HIR0903618.1"
                     /translation="MESERGTFYAVGVGPGDPELMTRKAVRVLEECPVLAVPRTPAGD
                     SLALEIAQAGADLTDKEIHFIDFAMSRDEEKRRQAHRRAAEAVRALLDRGTDVAMPVL
                     GDVSLFASSAYVAQLLEEEGCRCVRVPGVPSFCAAAARLGRSLTEMDLPVHIVPAGGF
                     PLEEALDLPGTKVLMKSGRALPQVLEALESRGLLESSAMVQNCGLPGEQAAESLKDAP
                     QSGSYFTTLLVP"
     gene            1521..2048
                     /locus_tag="ACZPAQ_06225"
     CDS             1521..2048
                     /locus_tag="ACZPAQ_06225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR0903619.1"
                     /translation="MGVSGLLLLFPFLLLRFGLLGRLGRDGLGRAAHIPPMGGRERLA
                     YWVYQLSSAGVLVCLCAQRAEVDGAWPFWAGLVLYLLGLALCAGAVVSFGRPSPGPFR
                     EDGLYRFSRNPMYVSYFVYFAGCVLLTRSRLLAALVLLFQLSEHWIILAEERWCREQF
                     GAPYEAYRKRVRRYL"
     gene            2074..>2772
                     /gene="cobM"
                     /locus_tag="ACZPAQ_06230"
     CDS             2074..>2772
                     /gene="cobM"
                     /locus_tag="ACZPAQ_06230"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR0903620.1"
                     /translation="MVHFVGAGPGAPDLITLRGAELLRGADCVIYAGSLVNPALLGLC
                     RDSARIYDSARMTLEQVLEAVERAEAAGQETVRLHTGDPCLYGAVREQMDALDRRGIP
                     WDVTPGVSSFCGAAAALGAEYTLPGVSQTVIITRMAGRTPVPQGEEIASLARHGATMV
                     IFLSAGMLEALSARLMEGGYRSDAPAALVYKATWPEEKVVRCTVGELAQAGRDHQISR
                     TALVVVGDILAGPYD"
BASE COUNT          392 a          855 c         1067 g          458 t
ORIGIN      
        1 cggccctcct gggggggctc ggggtgggcc gggtcacccg gccggggctg gaccagccgg
       61 tgggggaggc ggccatcaac tcggtgcccc ggaggatgat cgcccgggcg gcggaggaag
      121 aggcggcggc cctgggctac gccgggggac tggagatcac catctccgtt cccggggggg
      181 aggaggccgc ccgccggacc ttcaatcctc agctgggggt ggaggggggc atctccatcc
      241 tgggcacctc cggcatcgtg gagcccatga gcgcccaggc cctggtggag accatcgccc
      301 tggagctgcg ccagacggcg gcggagggga cggaccgggt ggtgctgacc ccaggcaact
      361 atggggccga ttttttggcg gagagcggcc tgatcacttc tgatatcagg atagtaaagt
      421 gttctaactt tatcggtgag gccctggacc gcgccgcgga ggcggggatc aggcagctga
      481 tcctggtggg ccatctgggc aagctggtga aggtggcggg gggcgtgatg aacacccact
      541 cccgctgggc ggactgccgc atggagatcc tgtgcgccca cgcggccctg tgcggggccg
      601 gccaggagac tgcccgggcc ctgatgggca gcgccaccac cgacgccgga ctggacatcc
      661 tggcggaggc cggcctgctg gaccggacca tggacagcct gctgtccgcc ctccaggcac
      721 acctggagcg gcgggcgggg acgtcctgcc gctgcggcgg cgtactgttc accaataccc
      781 ggggcctgct ggggatgacc gggactgcaa aggagatctt gaacatatgg agagcgaaag
      841 aggaacattt tacgccgtag gggtgggacc cggggacccg gaactgatga cccggaaggc
      901 ggtgcgggtg ctggaggagt gtcccgtcct ggctgtcccc cgcacgccgg cgggggactc
      961 cctggccttg gagatcgccc aggcaggtgc ggatctgact gacaaggaga tacactttat
     1021 agactttgcc atgtcccggg atgaggagaa acgccgccag gcccaccggc gagcggcgga
     1081 ggcggtgcgc gccctgctgg accgggggac agacgtggcc atgccggtgc tgggggacgt
     1141 gtctctcttc gccagcagcg cctatgtagc ccagctgctg gaggaggagg gctgccgctg
     1201 tgtcagggtc ccgggggtcc ccagcttctg cgccgccgcg gcccggctgg gccggagcct
     1261 gacggagatg gacctgccgg tccacatcgt cccggcgggg ggcttccccc tggaggaggc
     1321 cctggacctg ccggggacaa aggtgctgat gaagtccgga cgggccctgc cccaggtgct
     1381 ggaggcgctg gagagccggg gcctgctgga gagctcggcc atggtgcaga actgcggcct
     1441 gccgggggag caggcggcag agagcctgaa ggacgctccc cagtccggca gctacttcac
     1501 cacccttctg gtgccgtgac atgggcgtgt ccggtctgct cctgctgttc cccttcctgc
     1561 tgctccgatt tgggctcctg ggccggctgg gaagggacgg actgggccgc gccgcccaca
     1621 ttccccccat ggggggcagg gaacggctgg cctattgggt ctatcagctc tcctccgccg
     1681 gggtgctggt gtgtctctgc gcacagcggg cggaggtgga cggcgcctgg cccttttggg
     1741 cgggacttgt cctgtacctt ttggggctgg ccctgtgcgc gggggcggtg gtgagtttcg
     1801 gaaggccctc cccgggccct ttccgggagg acggcctcta ccggttctcc cgcaacccca
     1861 tgtatgtgtc ctatttcgtg tattttgcgg ggtgcgtcct gctgacccgc tcccggctcc
     1921 tggccgccct ggtcctgctg ttccagctct cggagcactg gatcatcctg gcggaggagc
     1981 ggtggtgcag ggagcagttc ggcgcaccct acgaggcgta tcggaagcgg gtcaggcggt
     2041 atctctgatc cgtgtgggaa ggaggctttt tccatggtcc attttgtagg ggccgggccg
     2101 ggtgcgcccg acctgatcac cctgcgggga gcggagctgc ttcggggggc ggactgcgtc
     2161 atctatgcgg gcagcctggt caacccggcc cttctggggc tgtgcaggga cagcgcgcgg
     2221 atctatgaca gtgcccgcat gacgctggag caggtgctgg aggcggtgga gcgcgcagag
     2281 gcggcggggc aggagacggt acgcctccac accggcgacc cctgtctgta cggtgcggtg
     2341 cgggagcaga tggacgccct ggaccggcgg ggcatcccct gggacgtgac cccgggggtg
     2401 tcctccttct gcggggcagc ggcagccctg ggggcggagt acaccctgcc cggggtgagc
     2461 cagaccgtta tcatcacccg catggcgggc cggacccccg tgccgcaggg ggaggagatc
     2521 gcctccctgg cccggcacgg ggccaccatg gtcatcttcc tctccgccgg gatgctggag
     2581 gcactgtctg cccggctgat ggagggggga taccggtcag atgctccagc tgccctggtg
     2641 tataaggcga cttggccgga ggagaaggta gtgcgctgca cagtgggaga actggcccag
     2701 gccgggcggg accaccagat ctccaggacc gcgctggtgg tggtggggga catcctggcc
     2761 ggaccctatg ac
//
LOCUS       DBMDSJ010000232         2771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8852_length_2771_cov_1.529213, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000232 DBMDSJ010000000
VERSION     DBMDSJ010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8852_length_2771_cov_1.529213"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="ACZPAQ_06235"
     CDS             complement(<1..808)
                     /locus_tag="ACZPAQ_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903621.1"
                     /translation="MSDLPYWILALVFLFAFPPVGVLMIALRLFGGGKRRVARGRHPY
                     YMQRDGQAPVGARTTVSEPARSQEKTGRREKKRQEGKAEQDMSPVCLDVVEKMAKRGR
                     RLIQVGGIITAVFAFTVLACLGDASYWLLHGDAIWFWDQLLNAIPLLCFLGGGAGVLW
                     AGLRKKKQVRTYRNYLAMIGRRQTISISTLASATGASPAKVRDCLEDMLDDGLFPLGF
                     LDYGGDRLVLSGEGLNDPAPAQEEKTEEKKAAPVDEENAVLAEIRAVNDSI"
     gene            1211..>2771
                     /locus_tag="ACZPAQ_06240"
     CDS             1211..>2771
                     /locus_tag="ACZPAQ_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016202.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR0903622.1"
                     /translation="MDGARVQKKSGGKPGPAVWVPAAILAVLLAAYLGLCGWVSASDT
                     ILPNVTAAGLDLSGMTQAEAEQAINENLSVAQQAGSGWAVDLSCDGYIGTLDGSSFQV
                     DAASLAQASLSVGRGSFFTGGAQYLRHLFGAAQDLGADQVQLTEAGRTELERQLQAAD
                     SALGSGGSEDGYTADLEAGTLTLVKGHTHRSVDRQGAEEAVKSAYAQLLAQGQADTVQ
                     LPILDSAPQEPDFQAIHGELYAEAANAAIDPDTCEVLPHTVGVDFDTAQAQRLFQQAQ
                     EGETVEVPLTVTQPDITQEILADRLFADLLGQGTSQVSGSSNRKFNVKLSAEACNGVI
                     LMPGEEFSYNNTTGSRSADKGYLPAPVYSGGASVDETGGGICQTSSTIYYAVLHTTLE
                     IVERHAHMYSVGYVPDGMDATVYFGLSDFRFKNNTDYPVKIVTESYDKNGLRYLTVKL
                     YGTNVDGRYAVPERTQFDFVSPTTQYRADESIPQGTTKVDAKQNAYTGRSARAWRVIY
                     EKDGTLVEKQDL"
BASE COUNT          522 a          952 c          795 g          502 t
ORIGIN      
        1 cgatggagtc gttcaccgcc cggatctcgg ccagcaccgc gttctcctcg tccaccgggg
       61 cagccttctt ctcctccgtc ttttcctcct gtgccggggc cgggtcgttc agcccctcac
      121 cggagaggac cagccggtcc ccgccgtagt ccaggaaacc cagggggaac agcccgtcat
      181 ccagcatatc ctccaggcag tcccgcacct tggccgggga ggcaccggtg gcggacgcca
      241 gcgtcgagat ggagatggtc tggcggcggc cgatcatggc cagatagttc cggtaggtgc
      301 gcacctgctt ctttttccgc aggccggccc acagcacgcc ggcaccgccg cccaggaagc
      361 agagcagggg gatggcgttc agcagctggt cccagaacca gatcgcgtcg ccgtggagga
      421 gccagtagga cgcatcaccc aggcaggcca gcaccgtgaa ggcgaagacc gccgtaatga
      481 tcccgcccac ctggatcagc ctccggcccc gcttggccat cttctccact acgtccaggc
      541 acaccgggga catatcctgt tccgctttcc cttcctggcg ctttttctcc ctccgaccgg
      601 tcttttcctg gctccgtgcc ggctcactga cagtggtccg ggcccccacg ggggcctgcc
      661 catcccgctg catataatag ggatggcgcc cccgggccac gcggcgcttc ccgccgccga
      721 agagcctcag agcgatcatc agcacgccca ccggcgggaa ggcaaacaga aagaccagcg
      781 ccaagatcca gtacggcagg tcactcatcc ctccgccaga gctcctgcgg ggctcccatt
      841 ttccgccgta gttctcattt ccctgatctc cgtaatatgc catcattctg cctcccctgg
      901 tgttctgctg tatagaatac cagacccatc ccaaaacaac attgattttt tcttaaaatt
      961 ccttgaaaat ttctcttctg ggtctggccc tctctcttcc acatacacca ttctatcata
     1021 ctgccgggaa ggtgaaaagt aaacctccta aaaatatttt tcaaaattga ggaaatccgg
     1081 ggaaatttgg acggtcaact gtaactgttc tgtaattaca ttctgaaacg taggatgcta
     1141 taatatagtc tgaaattatt tgacctgtcc gtgggttctt gggctgagtg ggacaggaag
     1201 gggagttttc atggacggag cacgtgtaca gaaaaaaagc ggcggaaagc ccggcccagc
     1261 cgtctgggtc ccggccgcca tcctggctgt cctccttgcc gcctacctgg gcctgtgcgg
     1321 ctgggtcagc gcctcggaca ccatcctgcc caacgtcacc gccgccggac tggacctgtc
     1381 cggcatgacc caggctgagg cggagcaggc catcaacgag aatctttccg tcgcccagca
     1441 ggctggctcc ggctgggccg tggacctgag ctgcgacggc tatattggta cgctggacgg
     1501 ctcatccttt caggtggacg ccgcctccct ggcccaggcg tccctgtctg tgggacgcgg
     1561 cagcttcttc accggggggg cccagtacct gcgccacctg ttcggcgctg cccaggacct
     1621 tggtgccgat caggtccaac tgacagaggc gggccgtaca gagctggagc gccagctcca
     1681 ggccgctgac agtgccctgg gcagcggcgg cagcgaggat ggctacaccg cagacttgga
     1741 ggcgggcacc ctgaccctgg taaaggggca cacccaccgc tcggtggacc gccagggagc
     1801 ggaggaggcg gtgaagtctg cctacgctca actcctggcc cagggtcagg cggacacggt
     1861 ccagctcccc atcctggatt ccgctcctca ggagccggac ttccaggcca tccacgggga
     1921 gctgtacgcg gaggcggcca acgcagccat cgatccggat acctgcgagg tgctgcccca
     1981 caccgtgggg gtggactttg acaccgccca ggcccagcgc ctgttccagc aggcccagga
     2041 gggagagacc gtggaggtcc ccctcaccgt cactcagccc gacatcaccc aggagatact
     2101 ggccgaccgg ctctttgccg acctgctggg gcagggtacc agccaggtga gcggctcctc
     2161 caaccggaag ttcaacgtga agctgtccgc tgaggcctgc aacggcgtga tcctgatgcc
     2221 cggcgaggaa ttctcctaca acaacaccac cggaagccgt tccgccgaca agggctatct
     2281 ccccgctccc gtctactccg gcggcgcctc cgtggacgag accggcggcg gcatctgcca
     2341 gacctcctcc accatctact acgccgtcct ccacaccacc ctggagatcg tggagcgcca
     2401 cgcccacatg tactccgtgg gctacgtccc tgacggcatg gacgccaccg tctacttcgg
     2461 cctgtccgac ttccgcttca aaaacaacac cgactacccg gtgaagatcg tcactgagag
     2521 ctacgacaag aacggcctgc gctacctcac tgtcaagctc tacggcacca atgtggacgg
     2581 ccggtacgcc gtgccggagc gcacccagtt cgactttgta tcccccacca cccagtaccg
     2641 ggccgacgag tccatcccac agggcaccac taaggtggat gccaagcaga acgcctacac
     2701 cggccgctct gcccgcgcct ggcgtgtcat ctatgagaag gacggcacct tggtggagaa
     2761 gcaggatctg g
//
LOCUS       DBMDSJ010000233         2724 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_8990_length_2724_cov_2.452535, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000233 DBMDSJ010000000
VERSION     DBMDSJ010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8990_length_2724_cov_2.452535"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="ACZPAQ_06245"
     CDS             complement(<1..423)
                     /locus_tag="ACZPAQ_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HIR0903623.1"
                     /translation="MGKGEELHMELEFDRDVIHCWEIAADGTLCQEETLETIVPDACP
                     DILRIVAVCGQATLNGKQAREGAAAVSGSVRAVLLYQPEEGGGLRRMEAALPFSAQLE
                     APGLTEQGRVHACVRLRGAEARALNPRKVLLRADLAVDV"
     gene            complement(739..2034)
                     /locus_tag="ACZPAQ_06250"
     CDS             complement(739..2034)
                     /locus_tag="ACZPAQ_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903624.1"
                     /translation="MTTGQRIAAKRKERELSQESLGAELGVSRQTVYKWESDTSLPEI
                     EKLVALSRLFQVPVGWLLGVEEVPEATEFSEDQLKLLEELLGRYQRVEQAELSQGQRA
                     QVEALVGERLSGSQRPRRRWPWALAALAVVWAGWSVFSRLDRMDGQYNSLANSVNNVS
                     HSVNVQVGSIASRVEEILKAQNDLTADYATQLVSADLAENTVRFSLRAVPKTYTPGMS
                     VVFLADSGGEVREMEAAEEENGAFTGEMVCPLTDSITLSAVFVTGNTRQTQLLDQYQG
                     LYQDSFPQVEPMDLSSVMYSKRGKNGRFALPEGYITVGGERASVPVVNEALGVSRLRE
                     VRVGLFHNFKLVTWLEPCEKPDSFEGFAGQDFYRLDPMELDLAEGDDLAFAAVVTDEY
                     GRRWVSPSIPEYQVQDGEILWPEESVVYQKNTLADWGLE"
     gene            complement(2120..2722)
                     /locus_tag="ACZPAQ_06255"
     CDS             complement(2120..2722)
                     /locus_tag="ACZPAQ_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR0903625.1"
                     /translation="MTIREAIVVEGRYDKNTLSQVVDTLILETNGFGIFKDPERMSLL
                     RRAAERRGLIVLTDSDGAGFVIRNRIRGSIPAEHLKHAYIPDVYGKEKRKRQRGREGK
                     LGVEGMSPQVLEQVLRRAGATVLDAQAPERARPGLTKADLMAAGLTGGADSARRRQAL
                     LKEMELPEHTSANALLQVLNGCYSREEALDLLGLKDLDGM"
BASE COUNT          445 a         1030 c          761 g          488 t
ORIGIN      
        1 cacatccacc gccagatcgg cccggagcag cacctttctt ggattcaggg cccgggcctc
       61 cgccccccgg agccggacgc aggcgtggac ccggccctgc tccgtcagcc ccggggcctc
      121 cagctgggcg gagaagggca gggccgcctc catgcggcgc agcccgcccc cctcctccgg
      181 ctggtagagg aggacggccc ggacgcttcc gctcaccgcc gccgccccct cccgggcctg
      241 cttcccgttc agtgtcgcct gtccgcacac cgccacgatg cggaggatgt ctggacaggc
      301 gtcgggcaca atggtctcca gggtctcctc ctggcagagc gtcccgtccg ccgcgatctc
      361 ccagcagtgg atcacatccc gatcaaattc caattccatg tgcagttcct ctccctttcc
      421 cataggatgg gcctccggtc gccggggccc gggccttctt tgggacacta tatgtccggg
      481 agcaggagaa tatgtccatc tgtctcagcc cttcagaccg aacagcctgc gcagcagccc
      541 gcgcaggcat ttgggcgaac gtaccaccac gatacccatt ccgcgaccct ccctccaaca
      601 gattgtagtc cagtatatgg cccggcagaa ccaaatgaca ccgccgggac gagccaaagc
      661 tccgggggcc gcctataaaa atgcagcgcc acccaaagag cggcgctgcg tttttccaat
      721 atgcgttgac ttccggactt actccaggcc ccagtctgcc agggtgtttt tctgatagac
      781 caccgattcc tccggccaga ggatctcccc atcctgcacc tgatactccg ggatcgaggg
      841 ggacacccac ctccgaccat actcgtcggt gaccacggcg gcgaaggcga gatcatcccc
      901 ctccgccaag tccagctcca tggggtccag ccggtagaag tcctgtcccg caaagccctc
      961 gaagctgtcc ggcttctcac agggctccag ccaggtcacc agcttaaaat tgtggaacag
     1021 gcccacccgg acctcccgca gccggctcac ccccagcgcc tcgttgacca cggggacgga
     1081 ggccctctcc ccgcccacgg tgatgtatcc ctccggcagg gcgaagcggc cgtttttccc
     1141 ccgcttcgaa tacatcacag aggacagatc catgggctcc acctgtggga aactgtcctg
     1201 gtacagcccc tggtactggt ccagcagctg ggtctgccgg gtgttcccgg tgacaaagac
     1261 ggcggagagg gtaatgctgt ctgtcagggg acagaccatc tccccggtaa aagctccgtt
     1321 ctcctcctcc gcagcttcca tctcccgcac ctccccgcca ctgtccgcca gaaataccac
     1381 cgacatcccg ggggtatagg tcttgggcac cgcccgcagg gagaatcgta ccgtattttc
     1441 tgccaaatcg gcggagacca gctgggtggc atagtcggcg gtcaaatcgt tctgggcctt
     1501 caggatctcc tccacccggc tggcgatgga gcccacctga acgttcaccg agtggctgac
     1561 gttattcacc gagttggcca gactgttata ctgcccgtcc atccggtcca gacggctgaa
     1621 tacgctccat ccggcccaga ccaccgccag ggcggccaag gcccagggcc accgccgccg
     1681 gggccgctgg ctcccggaca gcctctcccc caccagagcc tccacctgcg cccgctggcc
     1741 ctggctcagt tcggcctgct ccacccgctg atagcggccc agcagttcct ccagcagctt
     1801 cagctggtcc tcagagaact ctgtcgcctc cgggacctcc tccactccca gcagccagcc
     1861 cacaggcacc tggaacaggc ggctcagggc caccagcttc tcgatctccg gcagggaggt
     1921 gtcactctcc cacttataga cggtctgccg ggataccccc aactccgccc ccaggctctc
     1981 ctgggacagc tcccgctctt tccgcttggc ggcgatgcgt tgtcctgtgg tcatggttca
     2041 tcatctcctc gcctccaccc tagcaggcgg ccgctttttc tgccaccacc cagcggtttt
     2101 ctccctgcta acccggggtt tacatcccgt ctaagtcctt gagccccaac aggtccagcg
     2161 cctcctcccg ggagtagcag ccgttgagca cctggagcag ggcgttggcc gaggtgtgct
     2221 ccggcagctc catctccttc agcagggcct gccgccgccg ggcgctgtcc gcccctccgg
     2281 tgagccccgc ggccatcaga tccgccttgg tcaggccggg ccgggcccgc tccggggcct
     2341 gggcgtccag gacggtggcc cccgcccggc ggagcacctg ctccagcacc tggggtgaca
     2401 tcccctccac ccccagcttg ccctcccggc cccgctggcg cttccgcttc tccttcccgt
     2461 acacgtccgg gatataggcg tgcttcaaat gctccgccgg gatggagccc cggatccggt
     2521 tccggatcac aaagcccgcc ccgtcggagt cggtgagcac gataagcccc cgccgctccg
     2581 ccgcccggcg gaggagggac atccgctccg ggtccttgaa gatgccgaag ccgttggtct
     2641 ccaggatcag ggtgtccacc acctgggaga gtgtattctt gtcgtaccgc ccctccacca
     2701 cgatcgcttc tcgtatggtc agca
//
LOCUS       DBMDSJ010000234         2718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9010_length_2718_cov_2.093619, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000234 DBMDSJ010000000
VERSION     DBMDSJ010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9010_length_2718_cov_2.093619"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="spoIIP"
                     /locus_tag="ACZPAQ_06260"
     CDS             <1..979
                     /gene="spoIIP"
                     /locus_tag="ACZPAQ_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HIR0903626.1"
                     /translation="GLSPDLDRWGRLLLAQSALLSGGRPAVDAFLAEQAQAEEPPAEP
                     IDQEENDQEEPELSPVQHGEVVEYTNLGKDDGSYLTTDQIYVKNSTQGQEVDVAALAS
                     QPVDLKLGAGPQILIVHTHGSEAYTQDEQDVYVESDPYRTTDCTKNVVRVGEEMAQVF
                     RSQGFEVVHDTTLYDYPAYNGAYERSCAGVEKWLEQYPTIKLVLDVHRDALVSADGAA
                     YKLVSEVEGQKVAQVMLVVGSNDSGMEHPNWRENLALAVKLQLELTRDWGQLARPITL
                     RSSRYNQHLSPGSLLVEVGGHGNTLQEAIAGARLFAQSIGQVLKTMEEP"
     gene            complement(1152..2036)
                     /locus_tag="ACZPAQ_06265"
     CDS             complement(1152..2036)
                     /locus_tag="ACZPAQ_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0903627.1"
                     /translation="MKKTVSYSKSQLLDDLGRLALMVLASVIMAVNLKSFVQAGDLVP
                     GGFNGLTLLIQRVALRFWGLSVPFSAINFLLNAVPAVISFKLIGKRFTLFSCVVILCT
                     SLLTDLIPPMPITDDVLLICIFGGLINGFAISLCLRGRATSGGTDFISIALSQQYDVD
                     AWNYIFMGNVVMLAVSGALFGWDKALYSILFQFASTQVIKLLDPSGRRATLFIVTRRE
                     TAQAVWQQIRDTHHSATLFQGTGLYDGEERVMLYSVVGGNQVRALIHRVRQTDPHAFV
                     NVMRTDQVAGRFYRAPRD"
     gene            complement(2230..>2718)
                     /locus_tag="ACZPAQ_06270"
     CDS             complement(2230..>2718)
                     /locus_tag="ACZPAQ_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR0903628.1"
                     /translation="RVPFLGICLGMQMAVVEYARHMCGWADAHSSELDPATTHPVIDL
                     MPDQRGVTAKGGTMRLGAYPCKVVSRGSRTYQAYGAEEISERHRHRYEFNNEYRDALT
                     QAGLELAGLSPDGRLVEIVELPGHPWFVGVQFHPELKSRPNKAHPLFRAFIAAAKENR
                     SC"
BASE COUNT          528 a          830 c          938 g          422 t
ORIGIN      
        1 cgggctctct cccgacctgg accgatgggg ccggctgctg ctggcccagt ccgccctgct
       61 ctccggcggc cgcccggcgg tggacgcctt tctggcagag caggcgcagg cggaggagcc
      121 gcccgccgag cccatcgacc aggaggaaaa cgaccaggag gagccggagc tctccccggt
      181 ccagcacggc gaggtggtgg agtacaccaa tctggggaag gacgacggca gctacctcac
      241 caccgaccag atctatgtga agaacagcac ccagggccag gaggtggacg tggctgccct
      301 ggccagccag ccggtggacc tgaaactggg ggcggggccc cagatcctga tcgtccacac
      361 ccacggcagc gaggcctata ctcaggacga acaggacgtc tatgtggaga gcgaccccta
      421 ccgcaccacc gactgcacca aaaacgtggt ccgggtggga gaagagatgg cccaggtgtt
      481 ccgaagccag ggctttgagg tggtccacga caccaccctg tacgactacc ccgcctataa
      541 cggggcctat gagcgctcct gcgcaggggt ggagaagtgg ctggagcagt atcccacgat
      601 caaacttgtt ttggatgtcc accgggacgc cctggtgtcg gcggacgggg cggcctataa
      661 gctggtgagc gaggtggagg ggcagaaggt agcccaggtg atgctggtgg tgggcagcaa
      721 cgactccggg atggagcacc ccaactggcg ggaaaatctg gccctggccg tcaagctcca
      781 gctggagctc acccgggact ggggacagct agcccggccc atcaccctcc gctccagccg
      841 ctacaaccag cacctctccc caggttccct tctggtagag gtggggggac atggaaatac
      901 gctccaggag gccatcgccg gggcacgcct cttcgcccag agcatagggc aggtgctcaa
      961 aaccatggag gagccgtaaa aagggctgag ggcccccgct taatggcggg ggccctcagc
     1021 atgtcaaaaa agttacagtc tgccgcgcgc agggtttgtc cgccgcaggc ggacaaaccc
     1081 tgcgcacgcg aactctgtga ggcttattcc acaagctaaa ggccgcctac tccggggggc
     1141 ggccccttgc tttaatccct gggggcccgg tagaaccggc cggccacctg gtccgtgcgc
     1201 atcacgttga cgaaggcgtg ggggtcagtc tggcgcaccc ggtggatcag ggcccgcacc
     1261 tggttgcccc ccaccacgga gtagagcatc acccgctcct caccgtcata gaggccggtg
     1321 ccctggaaca gcgtggcgct gtggtgggtg tcccggatct gctgccagac cgcctgggcg
     1381 gtctcccgcc gggtgacgat gaagagggtg gcccgccggc cgctggggtc cagcagcttg
     1441 atcacctggg tggaggcaaa ctgaaagagg atggaataca gggccttgtc ccagccgaac
     1501 agagcgcctg agacggccag catcaccacg ttgcccataa atatataatt ccaggcatcc
     1561 acatcgtact gctgggagag ggcgatggag atgaagtcgg tgcccccgct ggtggctctc
     1621 ccccggaggc acaggctgat ggcgaagccg ttgatgaggc cgccgaagat gcagatgagc
     1681 agcacatcgt cggtaatggg catagggggg atcagatcgg tgagcaggct ggtgcacagg
     1741 atgaccacgc aggagaacag ggtgaaccgc ttgccgatga gtttgaagct gatgacggcg
     1801 ggcaccgcgt tgagcaggaa gttgatggcg gagaagggga cggacaggcc ccagaaccgc
     1861 agcgccaccc gctggatcag cagggtcagg ccgttgaagc ctcccggcac caggtccccg
     1921 gcctggacaa aactcttcag gttgacggcc atgatgacag aggccagcac catcagggcc
     1981 agccgcccca ggtcgtccag gagctgggat ttggagtaag agacagtttt tttcatggcg
     2041 tgacctcctg cgtgtttccc ggggaatgtg ctcccattgt atccgctccc ggtccctttg
     2101 gcaaggggga agggcggaaa agaccgggaa tgtgggaagt atccgacatc ccccctcccc
     2161 cgcagagagg gagcagccgc tccgcatccc cggacgggga gatgtggggt ggagggaaat
     2221 gtggaaaaat cagcaggagc ggttctcctt ggcggcggcg atgaaggcgc ggaacagggg
     2281 gtgtgcctta ttgggccggg atttcagctc cgggtggaac tgtaccccca cgaaccaggg
     2341 gtggccgggc agctccacga tctccaccag ccgcccgtcg ggggacaggc cggccagctc
     2401 cagccccgcc tgggtcaggg catcccggta ttcgttgttg aactcgtagc ggtgacggtg
     2461 ccgctcggag atctcctcgg ctccataggc ctgataggtg cgggagccgc ggctgaccac
     2521 tttgcagggg taggccccca ggcgcatggt accgcccttg gcggtgaccc cccgctggtc
     2581 gggcatcagg tcgatcaccg gatgggtggt ggcggggtcc agttcgctgg agtgggcgtc
     2641 ggcccagccg cacatgtgac gggcatactc caccaccgcc atctgcatgc ccaggcagat
     2701 gcccaggaag gggacccg
//
LOCUS       DBMDSJ010000235         2718 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9012_length_2718_cov_1.854031, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000235 DBMDSJ010000000
VERSION     DBMDSJ010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9012_length_2718_cov_1.854031"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            128..835
                     /locus_tag="ACZPAQ_06275"
     CDS             128..835
                     /locus_tag="ACZPAQ_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0903629.1"
                     /translation="MRILIVEDEVRLAEALAQIMAEQKYQVDQVHNGADGLDYALTAQ
                     YDVIVLDVMLPKLDGLEVARRLRSAHVSTPILMLTARDEISDKVKGLDCGADDYMTKP
                     FDTSELLARVRALTRRQGEVVSEELAFGDLSLHLSSHCLRRGDKSVRLGFKEFEVLRL
                     LLQNAKLVTPKDDIIAKVWGLDSEAEDNNVEVYISFLRKKLSFLASQVSIGTVRKVGY
                     FLTTSDEPGSFGGGGMP"
     gene            832..2127
                     /locus_tag="ACZPAQ_06280"
     CDS             832..2127
                     /locus_tag="ACZPAQ_06280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903630.1"
                     /translation="MIRTLRRKFILVNMLLVSLVLLMVFGVFMASSAQRLKDQSLLAM
                     RAALMWGEEGSPLRPEIAFPLDGSRRPSSGQGAQRSLLPVFTVTVEDGVVTAINDGDQ
                     VDVSLEVAQQAAQAALEDGRRDGTVSSLRYLREDAEGGAVRLAFADRTWERENLLALL
                     RTCLLVGLLGLACFFVISLLLSTLALRPAERSWQQQKQFVADASHELKTPLTVILANA
                     GILLAHSGDMADGQRKWVEYIQAEAQRMKSLVEDLLFLARSDDRRSPLRLQTVSFSDL
                     AWEALLPFESVAFEAGVALDSQITPDLELLGDPEQLRRLVAILVDNAVKYAGPRGSAS
                     LILRPQGDEAVLSVHNTGEPIPPEHLPRLFERFYRTDSDRARSQGGYGLGLSIAKSIV
                     DGHRGRISVSSSAISGTLFTVRLPLPGRGLGHLRTRSLG"
     gene            2410..>2718
                     /locus_tag="ACZPAQ_06285"
     CDS             2410..>2718
                     /locus_tag="ACZPAQ_06285"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HIR0903631.1"
                     /translation="MEFLWEGLLSITWQQLLMYVVGGLLIWLAIEKGFEPALLLPMGF
                     GAILVNLPLSGVINQFSEGVGETHGIIQWLFEVGIEASEAFPILLFIGIGAMIDFRPL
                     L"
BASE COUNT          460 a          864 c          844 g          550 t
ORIGIN      
        1 cccttgatgc tcttctgggg ccagtataac aggagaagct gaaattactc tgaatttttc
       61 agggcatttt cagttttttg tggtatagtg agaaaaaaca gagcccaatt gggaaaggag
      121 gcccattatg cgtatcctga tcgtggagga cgaggtccgg ctggcggagg ccctggccca
      181 gatcatggca gagcagaaat atcaggtgga ccaggtccac aacggtgccg acggcctgga
      241 ttatgcgctc accgcccagt acgatgtcat cgtcctggat gtgatgctgc ccaagctgga
      301 cgggctggag gtggcccgcc gcctccgctc cgcccacgtg tccactccca tcctcatgct
      361 caccgcccgt gacgagatct ccgacaaagt gaagggcctg gactgtgggg cggacgacta
      421 catgaccaag ccctttgaca cctccgagct gctggcccgg gtccgggccc tcacccgccg
      481 ccagggggag gtggtgtccg aggagctggc cttcggagac ctgtccctcc acctctcctc
      541 ccactgcctg cgccggggag acaagtcggt gcggctgggc ttcaaggagt tcgaggtgct
      601 gcgtctgctt ctccagaacg ccaagctggt cacccccaag gacgacatca ttgccaaggt
      661 gtgggggctg gactcggagg cggaggacaa caatgtggaa gtgtacatct ccttcctgcg
      721 gaagaagctg tccttcctgg cctcccaggt ctccatcggc accgtgcgga aggtgggcta
      781 tttcctcact acctcggatg agcccggcag ctttggcgga gggggcatgc catgatccgt
      841 accctgcggc gcaaattcat tctggtgaac atgctgctgg tgtccctggt cctccttatg
      901 gtctttgggg tctttatggc aagcagtgct caacgcctga aggaccagag cctgctggcc
      961 atgcgtgccg ccctgatgtg gggagaggaa ggcagtcccc tccggccgga gatcgctttt
     1021 cccctggatg ggtcccgccg cccctccagc ggccagggcg ctcagcgctc cctgctcccg
     1081 gtgttcaccg tcacagtgga ggacggcgtg gtcaccgcta tcaacgacgg agaccaagtg
     1141 gatgtctctc tggaagtggc ccaacaggcc gcccaggccg ctctggagga cggccgccgg
     1201 gatggcaccg tctcctccct ccgctatctc cgggaggatg cggagggagg cgcggtccgc
     1261 ttggcctttg ccgaccggac ctgggagcgg gaaaatcttc tggccctgct gcggacgtgc
     1321 ctgctggtgg ggctgctggg gctggcctgt ttctttgtca tctccctgct cctctccacc
     1381 ctggccttac gccccgccga gcggtcctgg cagcagcaga agcagttcgt agccgacgcc
     1441 tcccacgagt tgaagacccc cctcactgtc atcctggcca acgccggtat cctcctggcc
     1501 cactccggag acatggctga tggacagcgc aagtgggtgg agtacatcca ggcagaggcc
     1561 cagcggatga agtcactggt ggaggacctg ctcttcctgg cccgctctga tgaccggcgc
     1621 tctcccctcc gtctccagac ggtgtccttc agcgacctgg cctgggaggc cctgctcccc
     1681 tttgagtcgg tggcctttga ggccggcgtg gccctggaca gccagatcac gcctgatctg
     1741 gagctcctgg gcgatccgga gcagctgcgc cgcctggtgg ccattctggt ggacaacgcg
     1801 gtcaagtatg ccgggccccg cggctcggcc tcactgatcc tccgccccca gggagacgag
     1861 gcagtcctct ccgtccacaa caccggcgag cccatccctc cggagcatct gccccgcctg
     1921 ttcgagcgct tctaccgcac tgacagcgac cgggccagga gccagggggg ctacggtctg
     1981 gggctgtcca tcgccaagag catcgtagac ggccatcggg gccggatctc cgtctccagc
     2041 tccgccatct ccggcaccct cttcaccgtc aggctgcccc tgcccggccg cggcctgggg
     2101 cacctccgga cccgctccct ggggtgaatg gcccgccgtt tctgcatggt gaaaaaagcg
     2161 gagatttctc ttgacagccc caggggaaga tgataaaatc cgtaaataca aaggggagta
     2221 gtcggcgcgg ttcccgcgcg gaaaggccaa caagcatgga tggagcttgt tccattctgg
     2281 tttttcctga aatgaccaga cctgcgttcc aatgggaacg caggtctttt tttgcgggct
     2341 gtctgccggg cgcgggccga tttttctccg aaagggtaca catactataa agaaatggag
     2401 gaagaaagca tggaatttct ttgggagggg cttctgtcta tcacatggca gcagctgctg
     2461 atgtacgtcg tgggcggcct tttgatctgg ctggccatcg agaaggggtt tgagcccgcc
     2521 ctgctgctgc ccatgggctt cggcgccatc ctggtcaacc tgcccctgtc cggcgtcatc
     2581 aatcagttca gcgagggcgt gggtgagacc cacggcatca tccagtggct ctttgaggtt
     2641 ggcatcgagg ccagcgaggc cttccccatc ctgctcttca tcggcatcgg cgctatgatc
     2701 gacttccgtc ccctgctg
//
LOCUS       DBMDSJ010000236         2716 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9019_length_2716_cov_1.437859, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000236 DBMDSJ010000000
VERSION     DBMDSJ010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9019_length_2716_cov_1.437859"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            232..777
                     /gene="hpf"
                     /locus_tag="ACZPAQ_06290"
     CDS             232..777
                     /gene="hpf"
                     /locus_tag="ACZPAQ_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR0903632.1"
                     /translation="MKFVFTDKKVNLPAVIHRYAEKKVGKLDRFFKEDATAAVTFSIE
                     RDRNRVEVTIRSGGTIFRASESTSDMHASIDAAVATLDRQIRKNKTRLEKRLRQGAFE
                     RSVDEEISTFVPDGPEEGEYNLVRSKRFPIKPMTRDEAILQMNLLGHSFFAFRDEDAD
                     GAFAVVYCRNDGDYGLIEDEA"
     gene            888..1838
                     /locus_tag="ACZPAQ_06295"
     CDS             888..1838
                     /locus_tag="ACZPAQ_06295"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR0903633.1"
                     /translation="MNPKALIDLHCDTLTDCRPTPAGNPDTLDDPERVLSLSAMPEDV
                     HWAQFYAVFIPDEYRGQAAIDYFEQNRENFFRQMKKFSQRAAPCRTYADMKRAWAGNQ
                     TAAFLTIENGSALAGDLSRVQVLADQGVRAVTLVWNGENEIGSGHTTDHGLSAFGKAV
                     IPELERCGILVDVSHLNDPGFYDLLEVAQKPFLATHSNARAVCGHRRNLTDEMIREMV
                     DRDCLIGLNYFVKFLRDEGRVESLDDLYRHVSHFLELGAAKNLALGSDFDGADLPDYL
                     NSPAKAAGIYEYLLSRGIGQELADGIMFGNASRFFQTHLT"
     gene            1922..2389
                     /locus_tag="ACZPAQ_06300"
     CDS             1922..2389
                     /locus_tag="ACZPAQ_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AlbA family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR0903634.1"
                     /translation="MEGLDDLEFLSEYREDNRIEAKRAQGGLPHSLWETYSAFANTLG
                     GVILLGVAEVKDGSLYSVPLLDPEELVEEFWSIVQDPKLVSANVLRTEDVRLMRSGRN
                     TVVAIFVPRADFRQRPVYLGSDPYRGTYYRCGEGDRRSPRHEVEAMLQDRGND"
     gene            complement(2453..>2716)
                     /locus_tag="ACZPAQ_06305"
     CDS             complement(2453..>2716)
                     /locus_tag="ACZPAQ_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HIR0903635.1"
                     /translation="QDKQISPELERLMEGRQIYQHKPGAETIPLCADGTAFHVGTAAP
                     ILSEGDVLGCVIFAEDGESQSPDEIDYKLAQAAAAFLGKHMES"
BASE COUNT          542 a          770 c          907 g          497 t
ORIGIN      
        1 acggtgacgt ggtggtgatg agcggccatc ccctcaactg gctcagccgg gtctccgccg
       61 tggtggtgga cggccggcgg gtgacggaat gatgttctgt tagaagaggg aaactcttga
      121 gaaaaattca caaaaaagtc tagaattttt tgacgcctgt ggtataattg actcatcgga
      181 ggccggatct ccgatgaagt tggccctccg gggcgaaagg agcggaatgt tatgaagttt
      241 gtgtttacgg acaaaaaggt gaacctgccc gccgtgattc atcggtacgc ggagaaaaag
      301 gtggggaagc tggaccgttt ctttaaagag gacgccaccg cagctgtcac attcagcatc
      361 gagagggacc ggaacagagt ggaggtcacc atccggtctg gcggcaccat cttccgcgcg
      421 tcggagagca cctccgacat gcacgcatcc atcgacgccg ctgtggccac gctggatcgc
      481 cagatccgca aaaataagac ccggctggag aagcggctgc gccagggggc ttttgagcgc
      541 agcgtggatg aggagatctc cacttttgtg cccgacgggc cggaggaggg ggagtacaac
      601 ctggtccgca gcaagcgctt tcccatcaag cccatgaccc gggacgaggc catccttcag
      661 atgaatctgc tgggccacag cttcttcgct ttccgggacg aggatgcgga cggtgctttc
      721 gcggtggtct actgccgcaa tgacggagat tacggcctga tcgaagacga ggcctgaccc
      781 agcttgaata gagcggaaag ggccggacgg acagaagtcc gcccggccct tcttccgtga
      841 tccaaaacgg gaggggagtt tccctccgga cagaatggag tggctttatg aatcccaaag
      901 cgctgatcga tctgcactgt gacacgctga cagactgccg gcccacccct gcggggaatc
      961 cggacacgct ggacgacccg gaacgggtgc tgtccctgtc cgccatgccg gaggatgtgc
     1021 actgggctca gttttatgcg gtctttattc ccgatgagta tcgaggacag gcggccatcg
     1081 actattttga gcagaaccgg gagaactttt ttcgccagat gaagaagttt tctcagcggg
     1141 cagccccctg ccgcacctat gcggacatga agcgggcatg ggccggaaat cagacagcgg
     1201 cctttctcac catcgagaac ggatcggcgc tggccggaga cctgagccgc gtccaggtgc
     1261 tggcagacca gggggtgcgg gcggtgacgc tggtgtggaa cggcgagaat gagatcggct
     1321 cgggccacac cacggaccac gggctgtccg ccttcggaaa agcggtgatc ccagagctgg
     1381 agcgatgcgg catcctggtg gacgtgtctc acctcaacga cccgggcttc tatgatctgc
     1441 tagaggtggc ccaaaagccc tttctggcca cccattccaa cgccagggcg gtgtgcggcc
     1501 acagacgcaa cctcacagac gagatgattc gggagatggt ggaccgggac tgtctgatcg
     1561 gcctgaacta ctttgtgaaa ttcctgcggg acgagggcag ggtggagagc ttggatgacc
     1621 tgtaccgcca tgtgtcccac ttcctggagc tgggggcggc caaaaacctg gccctgggct
     1681 ccgactttga cggggccgac ctgccggact acctgaacag cccggccaag gcggcgggga
     1741 tctatgagta tctgctgtcc cggggtatcg gccaggaact ggccgacggg atcatgttcg
     1801 gcaacgccag ccgctttttc cagacccatt tgacgtaaca agacgccccc tccctccacg
     1861 gagggggcgg acttcttctg tagggcgtga gtgccgacac gctagagaaa ggagcgaagt
     1921 gatggaaggg ctggatgatc tggaattttt gtcagagtac cgggaggaca accgtatcga
     1981 ggccaagcgg gcccagggcg gcctgcccca cagcctctgg gagacctact ccgctttcgc
     2041 taacaccctg ggcggggtga tcctgctggg ggtggcggag gtgaaggacg gctccctgta
     2101 ttcggtgcca ctcctggacc cggaggagct ggtggaggag ttctggtcca tcgtgcagga
     2161 ccccaagctg gtcagcgcca acgtgctccg gacggaagac gtgcggctga tgcggagcgg
     2221 gcggaacacg gtggtggcca tctttgtgcc cagggcagat ttccgccagc ggccggtgta
     2281 tctgggctcc gacccctatc ggggcaccta ctaccgctgt ggggaagggg accgccgcag
     2341 cccccggcac gaggtggagg cgatgctcca agaccgggga aacgactgag cgtagggaga
     2401 atagcgagga cgcagggggg agccctgcgt cctcgctgta tccaggagct gatcagctct
     2461 ccatgtgttt tcccaggaag gcggccgccg cctgggccag cttgtagtcg atctcgtcgg
     2521 gagactggct ctccccgtcc tccgcaaaga tgacgcagcc cagcacatcg ccctcggaca
     2581 ggatgggggc ggcagtgccc acatggaacg cggtcccgtc ggcgcagagg ggaatggtct
     2641 cggctcccgg cttgtgctga tagatctgac ggccctccat cagccgctcc agctcagggg
     2701 agatctgctt gtcttg
//
LOCUS       DBMDSJ010000237         2712 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9031_length_2712_cov_1.836844, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000237 DBMDSJ010000000
VERSION     DBMDSJ010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9031_length_2712_cov_1.836844"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(125..201)
                     /locus_tag="ACZPAQ_06310"
     tRNA            complement(125..201)
                     /locus_tag="ACZPAQ_06310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(165..167),aa:Pro,seq:cgg)
     gene            316..1326
                     /locus_tag="ACZPAQ_06315"
     CDS             316..1326
                     /locus_tag="ACZPAQ_06315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR0903636.1"
                     /translation="MYSQSTQAARRRRPAAQGRRTGKQEESGRRERVRLVQLLACLAL
                     FLTVFIGKGVFPQRLDQLGEDILALIGSNTDFRAAFSDLGESLAEQDSVLGDIGEFCI
                     QVFGSGQDSGGEASPALAGGVEEESRFLNSRPDMAELTAHYLRLDRVPDSWLPGEQAE
                     GEAAQQTPQAQTAAPAVGTVLLKANYNGPELPANYTMDQISLGDLETVTPVFGPLWST
                     YGYRDHPIDGEYRFHNGVDIGAYQGDPILAFAAGTVEYVGENDDHGLYFQIDHGNGIK
                     SFYAHCSRICVTTGQTVSAGEKVAEVGSTGNSTGPHLHLELKYAGTHLDPAYYIDYKQ
                     AV"
     gene            1327..1947
                     /locus_tag="ACZPAQ_06320"
     CDS             1327..1947
                     /locus_tag="ACZPAQ_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR0903637.1"
                     /translation="MGRGRITVTPGFLFLMAWCNYADQQGLLLPALAGCALHELGHLA
                     AIWGLGGSVRRLRLTAVGAEIQLERPMSYPGELAAALAGPAANLAAAWLCCRAEGGAL
                     FAGLNLALGCFNLLPVGRLDGGRALHCLLSMSIGPAAEEAEAVLDRVFIAFLLALGVL
                     LIGWGGSFTLLLVALWLLAKEKEGKKGRNRSCQGLQKRVKWFLHSK"
     gene            1968..>2712
                     /locus_tag="ACZPAQ_06325"
     CDS             1968..>2712
                     /locus_tag="ACZPAQ_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family protein"
                     /protein_id="HIR0903638.1"
                     /translation="MNRTLERILPKVQKPARYTGGEYNAVVKDRAQVDTRYALCFPDT
                     YEIGMSNLGVRILYGAMNQVEGLWCERAFAPWGDMEEEMRREGIQLYGLESGDPLCDF
                     DIIGFSLGYEMAYTNVLNMLDLAGLPLRSEERRDLAPLVVAGGTCAYNPEPLAPFVDF
                     FVLGEGEEVTLEYIALYRRARDEDWTKEELLREAARIPGIYVPSFYEPVYRPDGTLEE
                     MRIREGSGAPELVRKRVVEDMNKSYFPVKT"
BASE COUNT          527 a          751 c          935 g          499 t
ORIGIN      
        1 cacgacagac tacaaagtat actactatta ttaacagaat aatagcatat taagtgagag
       61 ttttcaaata taacattccc aatgtctaaa taaaaaatgg gtgagtccta taaggactca
      121 cccatggtcg gagtgccggg attcgaaccc ggggcctctt ggacccgaac caagcgcgat
      181 accaaacttc gccacacccc gatgcagtta gcttatctat tatattttct tttctaagat
      241 ctgtcaagag gaatatagca tcttttctcg gaatagattg ggggtatcca tcaaacagga
      301 cagggagggg catctatgta cagccagtcc acacaggcgg cgcgtcggag gagaccggcc
      361 gcgcagggga ggcgcacagg gaaacaggag gagagcggca ggagggagcg tgtccggctg
      421 gtccagctgc tggcgtgtct ggcactgttc ctcaccgtgt tcatcggaaa gggagtcttt
      481 ccccagcggc tggaccagct gggggaggat atcctggcgc tgatcggctc caacactgat
      541 ttccgggcgg cattttccga cctgggtgag tcgctggcgg agcaggactc cgtgctggga
      601 gacatcgggg agttctgcat ccaggtgttc gggtccgggc aggactccgg cggagaggca
      661 tctcccgccc tggctggcgg ggtggaggag gagagccgct tccttaacag ccggccggac
      721 atggcggagc tcaccgccca ctatctgcgg ctggaccggg tgcccgacag ctggctgccg
      781 ggggagcagg cggagggaga ggcagcccag cagacaccgc aggcccagac ggcagcgcct
      841 gcggtgggaa cggtgctgct gaaagccaat tataacggcc cggagctgcc ggccaactat
      901 actatggacc agatctccct gggggacctg gagaccgtga ccccggtgtt cggccccctc
      961 tggtccacct acggctaccg ggaccacccc attgacggag aatacagatt tcacaacgga
     1021 gtggatatcg gcgcctatca gggggacccc attctggcat tcgccgcagg cacggtggag
     1081 tatgtggggg agaacgacga ccacggactg tatttccaaa tcgaccacgg caacggcatc
     1141 aaatcatttt acgcacattg cagcaggatc tgtgtgacca cgggacagac cgtgtccgcg
     1201 ggggagaagg tggcagaggt ggggtccaca gggaactcca ctggtcccca cctgcacctg
     1261 gagctgaaat acgcggggac ccatctggac ccggcatact atatcgacta caagcaggcc
     1321 gtctgagtgg ggcgggggag gatcacggtc acgcccggat tcctgttcct gatggcctgg
     1381 tgcaactatg cggaccagca ggggctcctg ctcccggccc tggcagggtg cgcgctccac
     1441 gagttgggcc acctggcggc catctggggg ctgggcggat cggtgcggcg cctgcggctg
     1501 acggctgtgg gggcggagat ccagctggag cggcccatgt cctatccggg ggagctggca
     1561 gcggcgctgg cgggcccggc ggccaacctg gcggcggcct ggctgtgctg ccgggcagag
     1621 ggcggggccc tcttcgccgg gctgaatctg gcgctgggct gcttcaatct gcttccagtg
     1681 ggcaggctgg acgggggacg ggcgctgcac tgtctgctgt ccatgtccat cgggccggct
     1741 gcggaggagg cggaggcggt cctggaccgg gtcttcatcg cctttttgct ggcgctgggc
     1801 gtgctcctga tcggctgggg aggcagtttt accctcctcc tggtggcgct ctggctgctg
     1861 gcaaaagaaa aagaggggaa aaagggaaga aataggtctt gtcaagggct tcagaaacgg
     1921 gtaaaatggt tcctgcactc gaaatgagag ttacctggag ggacgtcatg aaccggacac
     1981 tggagcgtat tctgccaaaa gtacaaaagc ccgcccgtta caccggcgga gaatataatg
     2041 cggtggtgaa ggatcgggcc caggtggata cccggtatgc cctctgcttt ccagacacct
     2101 atgagatcgg tatgtccaac ctgggggtgc gtatcctgta tggggccatg aaccaggtgg
     2161 agggcctgtg gtgcgagcgg gcttttgctc cctggggaga catggaggag gagatgcgcc
     2221 gggagggcat ccagctctac ggcctggaga gcggtgatcc tctgtgcgac tttgacatta
     2281 tcggattctc gctgggctac gagatggcct acaccaatgt gctgaacatg ctggatctgg
     2341 cgggcctgcc tctgcgctcg gaggagcggc gggacctggc cccgctggtg gtggcggggg
     2401 ggacctgcgc ctataacccg gaacccctgg ccccctttgt ggacttcttc gtgctggggg
     2461 agggggagga ggtcaccctg gagtacatcg ccctctaccg ccgggcccgg gacgaggact
     2521 ggaccaagga ggagctgctc cgggaggcgg cccgcatccc gggcatctat gtgccctcct
     2581 tctacgagcc ggtctaccgc cccgacggaa ccctggagga gatgcgcatc cgggagggct
     2641 ccggcgcgcc ggagctggtg cgcaagcggg tggtggagga tatgaacaag tcctatttcc
     2701 cggtgaagac ca
//
LOCUS       DBMDSJ010000238         2691 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9099_length_2691_cov_1.628571, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000238 DBMDSJ010000000
VERSION     DBMDSJ010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9099_length_2691_cov_1.628571"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="ACZPAQ_06330"
     CDS             complement(<1..403)
                     /locus_tag="ACZPAQ_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0903639.1"
                     /translation="MIFRCSDQGCGYFGEGPRLPEFCPRCGKRMLQAAEGEMTGDDWS
                     ALGVFWISRPDGKERGLACFRRSAGMGSGWGTCNLGICMEQGIGVEADPRQAFWLYQQ
                     AVEMGSLSAVCNLGVCYEQGIGTTSDQKQAVE"
     gene            complement(428..1891)
                     /locus_tag="ACZPAQ_06335"
     CDS             complement(428..1891)
                     /locus_tag="ACZPAQ_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR0903640.1"
                     /translation="MARSIMIQGTMSNAGKSVLAAGLCRIFRQDGYSVAPFKSQNMAL
                     NSFITREGLEMGRAQVMQAEAAGVEPSVRMNPVLLKPTSDVGSQVIVNGEVVGSMSAA
                     AYLSYKKQLIPTILESYHALAQTHDIVVLEGAGSPAEINLKAEDIVNMGMARMAGAPV
                     LLAGDIDRGGVFAALYGTMALLEPDERAMVKGTIINKFRGDVEILRPGLTQLEELTHV
                     PVVGVVPWMELDLDEEDSLSQHFSARREGKPLDVAVIRLPRISNFTDFAPLERHPAVG
                     LRYVDRVQALGRPDLVILPGTKSTLADLSWLRQSGLEGTILRLVGKGVPVIGICGGFQ
                     MLGRTLEDPQGVEGGGSMHGMGLLPVATTFSRKKSREQKYCQADLPDGFYGRLRGCPL
                     EGYEIHMGTSGGESIFTVEGRVLGTYLHGLFDSGAFTCGLVDLLLDEKGVEWNGEQSC
                     TEDARTFKERQYDRLADMLRRSLDMEAIYRILEQGLE"
     gene            complement(1884..2690)
                     /locus_tag="ACZPAQ_06340"
     CDS             complement(1884..2690)
                     /locus_tag="ACZPAQ_06340"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0903641.1"
                     /translation="MVRALRPDHALVLAPTFSEYHRALYKNNCKVKFHYANMHEDFRF
                     SSALLGKISQEIDAVFLCDPNNPTGTLISHQLLRALLARCREAGALLVVDQCFLELSP
                     GEPDRLVRELECGGLLLLRALTKSYAMAGLRLGYCLSDDRALLAAMAEEGPPWPVSQL
                     AQQAGISALQYAPDWPFRVRQQLEEERRYLSQELSRLRFHVYPSDSNFLLFQGEEGWR
                     ERLLDRGILIRSCADFQGLGPGFYRVCVRSRGENKRLIQAIQQEREAFHG"
BASE COUNT          520 a          944 c          739 g          488 t
ORIGIN      
        1 gctccaccgc ctgtttctgg tctgaagtgg tgccgatccc ctgctcatag cagacgccca
       61 gattgcacac cgccgacagg ctccccatct ccacggcctg ctgataaagc cagaaagcct
      121 ggcgggggtc ggcctccact ccgatcccct gttccataca gatgcccagg ttgcaggtcc
      181 cccagccact tcccatgccg gcggagcggc ggaagcaggc cagtcccctc tctttcccat
      241 cgggccggct gatccagaag acaccaaggg cggaccagtc atctccggtc atctccccct
      301 ccgccgcctg gagcatccgc ttgccgcagc ggggacagaa ctccggcagc cggggcccct
      361 ctccaaaata gccgcacccc tggtctgaac agcgaaaaat catactgctc tcctttctct
      421 ggccagccta ctccaggccc tgctccaaaa ttcgatagat ggcctccatg tccaagctcc
      481 gccgcagcat gtcagccagc cggtcatact gccgttcctt aaaggtacgg gcgtcctcgg
      541 tgcaggactg ttctccgttc cactccaccc ccttctcatc caggagcaga tccaccagcc
      601 cgcaggtgaa cgcaccgctg tcaaacagtc cgtggagata ggtcccaagc acccggccct
      661 ccacggtgaa gatgctctct cccccgctgg tccccatatg gatctcatag ccctccagag
      721 gacaccccct gagccgtcca taaaatccgt cagggagatc cgcttgacaa tacttctgtt
      781 ctctggactt tttacgagaa aaggtggtgg ccaccggcag gagtcccatc ccatgcatgg
      841 agccgcctcc ctccacgccc tgggggtcct ccagagtgcg gcccagcatc tggaatccgc
      901 cgcagatccc gataactggg acaccctttc ccaccagccg gaggatcgtc ccctccagcc
      961 cgctctgccg aagccaggac aggtcggcca gggtgctctt cgtacctggg aggatgacca
     1021 gatccggcct tcccagtgcc tggaccctgt ccacatagcg cagtcctacc gccggatggc
     1081 gctccagagg ggcgaaatcg gtgaagttgg agatgcgtgg caggcggatc accgccacgt
     1141 ccaggggctt cccctcccgt ctagcagaaa agtgctggga gaggctgtcc tcttcatcca
     1201 gatccagctc catccagggc accaccccta ccaccggcac atgggtcagc tcctccagct
     1261 gggtcagacc ggggcggagg atctccacgt cgccccggaa tttgttgatg atagtcccct
     1321 tcaccatagc ccgctcatca ggctccagca gggccatggt cccgtaaagg gcagcaaaga
     1381 caccgccccg gtcgatgtca cccgccagca gcacaggcgc ccccgccatc cgggccatcc
     1441 ccatattcac gatgtcctcc gccttcaggt tgatctctgc ggggctgccg gccccctcca
     1501 gtacgacgat atcgtgggtc tgggccaggg cgtggtagga ctccagaata gtggggatca
     1561 gctgcttttt gtaggacaag taggcagcgg cggacatgct gcccaccacc tccccgttga
     1621 cgatcacctg gctccccaca tcgctggtgg gcttgagcag gacgggattc atccgcacag
     1681 agggctccac gccggccgcc tccgcctgca tcacctgggc ccgccccatc tccagtccct
     1741 cccgggtgat gaaactgttc agcgccatat tctggctctt gaagggagcc acagagtacc
     1801 cgtcctgccg gaagatacgg cacagccccg ccgccagaac gctctttccc gcattggaca
     1861 tggtcccttg gatcataatg gatctagcca tggaaagcct ccctctcctg ctgaatggcc
     1921 tggatcagcc tcttattttc cccgcggctc ctgacgcaga cgcggtagaa gccaggaccc
     1981 agcccctgga agtcggcgca gctccggatc aggatgcccc ggtccagcag gcgctcccgc
     2041 cagccctcct ccccctggaa cagcaggaaa ttggagtcgc ttgggtagac gtggaaccgc
     2101 agcctgctca gttcctgaga gagatagcgg cgctcctcct ccaactgctg gcggacgcga
     2161 aatggccagt ccggcgcata ctgtaaagca gatatccctg cctgttgtgc cagctgtgac
     2221 accggccagg gcgggccctc ctccgccata gcggccagca gtgcccggtc atcggacagg
     2281 cagtaaccca gccgcagccc ggccatggca tagctcttgg tcagggcccg cagcagcagc
     2341 aaccctccgc actccagctc ccgaaccagc cggtccggct cccctggaga caactccaga
     2401 aagcactgat ccaccaccag cagtgctccg gcctcccggc agcgggccag aagggcccgc
     2461 aacagctggt gagagatcag tgtgcctgtg gggttgttgg gatcacagag gaacacagca
     2521 tcaatttcct gggaaatttt tccgagcagg gcgctggaaa agcggaaatc ttcgtgcata
     2581 tttgcgtaat ggaatttaac tttacaatta tttttgtaca gggccctgtg gtactccgaa
     2641 aaggtcgggg ccaggaccag ggcgtggtcc ggacgcaggg ctcggaccag c
//
LOCUS       DBMDSJ010000239         2674 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9156_length_2674_cov_2.085115, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000239 DBMDSJ010000000
VERSION     DBMDSJ010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9156_length_2674_cov_2.085115"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="ACZPAQ_06345"
     CDS             complement(<1..279)
                     /locus_tag="ACZPAQ_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003396712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HIR0903642.1"
                     /translation="MLLVNIEYIPGKEIEALGMVKGTVVQSKNFGKDFMAGMKTLVGG
                     EITGYTEMLNEARQIAVKRMVDEAEALGADAVINVRYGSSSVMQGAAEV"
     gene            complement(260..532)
                     /locus_tag="ACZPAQ_06350"
     CDS             complement(260..532)
                     /locus_tag="ACZPAQ_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903643.1"
                     /translation="MPKNIDPGQRAQKKDARKGAAFFALVQLAMALLLLWGRSQLAGL
                     EGLDWLRLLLLILAVGDLVTIPAVWIVLKQRMKEIEGGELDAARKY"
     gene            complement(516..776)
                     /locus_tag="ACZPAQ_06355"
     CDS             complement(516..776)
                     /locus_tag="ACZPAQ_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903644.1"
                     /translation="MRSNTDRKPSRLAPVLAALGTILVVFILVGTMLWAFLSAGGADT
                     IAVGVMALYGVVGLAVVGGVLAALIQRLREIKRGEEEDAKKY"
     gene            complement(760..1413)
                     /locus_tag="ACZPAQ_06360"
     CDS             complement(760..1413)
                     /locus_tag="ACZPAQ_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR0903645.1"
                     /translation="MKINEVEALVGITKKNIRFYEEQGLLKPGRNSQNGYREYGEPEV
                     ELLRQIKLLRKLGVPLEEIRQMQTGSRTLGDGMRRHLVTLERERKNLDQAVQFCQALK
                     DREVRLDSLDAGGLLREMEQMEQEGATFLDRQQGDTRRIRYVAPVVVTLLTVFLMGGL
                     IALMLWGFSIDAEDAPPLPLLVVLVAIPGVVILGVLLAMIQRFREIEKGEAEDAKQY"
     gene            complement(1484..2278)
                     /locus_tag="ACZPAQ_06365"
     CDS             complement(1484..2278)
                     /locus_tag="ACZPAQ_06365"
                     /inference="COORDINATES: protein motif:HMM:NF021592.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HIR0903646.1"
                     /translation="MEGYGTMPGAAMACMTASLLIAFGPPLGLGLFFRRRGGRWRAFL
                     LGAAVFLVFALVLEQRAHQLILGGPAGGVITGDLGLYALYGGLMAGLFEETGRFAAFQ
                     LLRRSGKGGTPQDALIYGAGHGGMEAVLLVGLTNVSNLLLSFWLNTGTLEQHMGQLDD
                     AAAQALAAAVAAPPHTYLWGGLERIVAVAIHISLSVLVWRAVERRASLWLFPAAIALH
                     AGVDACAILAAAHMPLAGVELVALVWAVALAVLARWIWRQREKKSL"
BASE COUNT          467 a         1018 c          695 g          494 t
ORIGIN      
        1 gacctcagct gccccctgca tcacagagga ggagccgtag cgcacgttga tgaccgcgtc
       61 ggcccccagg gcctccgcct catccaccat ccgcttcacc gcgatctgcc gggcctcgtt
      121 gagcatctcc gtatagccgg tgatctcgcc gcccaccagc gtcttcatac ccgccatgaa
      181 gtcctttcca aaattcttgg actgcaccac agtccccttc accatgccca gtgcctcgat
      241 ctcttttcca gggatatatt caatatttac gagcagcatc cagctctcct ccttcaattt
      301 ctttcattcg ttgcttcagc acgatccaca cggctgggat ggtcaccagg tcgcccactg
      361 ccaggatcaa caggagcagc cgcagccagt ccagcccctc cagtccggcc agctgggatc
      421 tgccccacag caggagcagt gccatggcca gctggaccag ggcaaaaaag gccgcgccct
      481 tcctggcgtc cttcttctgg gcgcgctggc ccgggtcaat attttttggc atcctcttcc
      541 tcccctcttt taatttccct cagccgctgg atcagggcgg ccagcactcc tcccaccacc
      601 gccagcccca ccacgccgta cagggccatg acgcccaccg cgatggtatc cgccccgcct
      661 gcgctgagga aggcccacag catggtgcct accaggatga acaccaccaa aatggtgccc
      721 agcgccgcca ggaccggggc cagccggctg ggttttcggt cagtattgct tcgcatcctc
      781 cgcctctcct ttctcgatct cccgaaatcg ctggatcata gccagcagga cccccaggat
      841 caccacgccc gggatagcca ccagcaccac cagcaggggc agcgggggcg catcctccgc
      901 atcgatggag aagccccaca gcatcagggc gatcagcccc cccatcagga acacggtgag
      961 cagcgtcacc accaccgggg ccacataccg gatcctgcgg gtgtctccct gctggcggtc
     1021 cagaaaggta gccccctcct gctccatctg ttccatctcc cggagcagtc cccccgcgtc
     1081 cagactgtcc aaccggacct cccggtcctt cagcgcctga cagaactgga ccgcctggtc
     1141 cagattcttc cgctcccgct ccagcgtgac cagatgccgg cgcatcccgt cccccagggt
     1201 ccggctgccc gtctgcatct gccggatctc ctccaggggg actcccagct ttctcagcag
     1261 cttgatctgc cgcaggagct ccacctccgg ctcaccgtac tcccgatagc cgttctggct
     1321 gttccgtccc ggcttcagca gcccctgctc ctcatagaag cggatgttct tctttgtgat
     1381 gcccaccagg gcctcgactt cgttgatctt catcggtatc cccctcgctc tgtggttagg
     1441 ttataccctc cagcgagggg aaggtcaagc attttccgtc atttcataaa gattttttct
     1501 ctctctgccg ccagatccac cgtgccagca cggccagggc cacagcccaa accagcgcca
     1561 ccagctccac ccccgccagg ggcatatggg ccgcggccag gatggcacag gcgtccaccc
     1621 ccgcgtggag ggcaatggcc gcggggaaga gccacaggga cgcccggcgc tccacagccc
     1681 gccagaccag caccgacagg gagatgtgga tggcaacggc cacgatccgc tccagcccgc
     1741 cccacagata ggtgtggggc ggcgcggcca ccgcagccgc cagcgcctgg gccgccgcgt
     1801 catccagctg gcccatgtgc tgctccagcg tgcctgtgtt cagccagaag gagagcagca
     1861 ggttgctgac gttggtcagc cccaccagca gcaccgcctc catgccgccg tggcccgccc
     1921 cgtagatcag ggcgtcctgg ggcgtgcccc ccttcccgct ccgcctcagc agctgaaagg
     1981 cggcaaatct ccccgtctcc tcaaacagcc ccgccatcag gcccccatac agtgcgtaga
     2041 gtcccagatc gcccgtaatg accccgcccg cagggccgcc caggatgagc tggtgggctc
     2101 tctgctccag caccagggca aataccagaa acaccgccgc ccccagaaga aaggcccgcc
     2161 agcgcccgcc tctccggcgg aaaaacagcc ccagtcccag gggcgggcca aaggcgatta
     2221 gcagagaggc cgtcatgcag gccatggcgg ccccgggcat cgttccgtat ccttccattt
     2281 ttatccctcc tttttcgacc ggccccggcg ttttgtcccg gccggctttc gggcccaccc
     2341 tacccctttc ccccggtaga aagtcaaggg tttctgcaaa aaaagtttcc ttccccctgg
     2401 tggaaacttg tgtttctgcc ggttttaccc ctgtttttcc caaaaacggg tgccccggcg
     2461 cgctctgcat tgagatgcgg gcccggagag gaaaacagcg gacgcctgcc atgcgcccgc
     2521 cgttctctat ccggaacctc ggccctgtac gcgccgcggt ccttcctgtt ctctgtcagc
     2581 ccttctcttt tacttccacc accgtcagcc gctctccccg cacctggaaa cggatctccc
     2641 agccggcgaa ctccatgccg tagatccgct ccgg
//
LOCUS       DBMDSJ010000240         2673 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9160_length_2673_cov_1.739114, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000240 DBMDSJ010000000
VERSION     DBMDSJ010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9160_length_2673_cov_1.739114"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            44..1360
                     /locus_tag="ACZPAQ_06370"
     CDS             44..1360
                     /locus_tag="ACZPAQ_06370"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0903647.1"
                     /translation="MPTPLYDALRGYAAKAPLRFHMPGHKGAFLPCPELAPLARLDVT
                     EIPSTGNLYEDLSPIREAQALWARRMGAEYCQFLTGGSTMGIHTALTLCCPPGSRVLV
                     DRSCHRSTFHALALLDLDPIFLPRPWLAEEGVAGPFSPLDVKKMLDQDPTIKTVCITS
                     PTYCGLLSDIPAIAGVVHAHGGKLVVDGAHGAHLQFLGMDAYRGADAVVVSAHKTLPA
                     MGQAALLFTNGIDPGQVRRTAAIYGTSSPSYPILASLDTAREWLEGPGAGEYGRVARR
                     TAALRCRFPSLCPPLPLDPTRFTLKVKNGPAFTGQLESRCNIWPEMEDGGHVVFILTA
                     CDGEEALDRLESVLEGMEEQMGDSAPLPPPPLPERVLSPRQAQFAPAEVLPLERTEGR
                     VCTCQLAPYPPGVPVVAPGERIGKKELAYFRQIGYNNREVTVAAMP"
     gene            1394..2317
                     /locus_tag="ACZPAQ_06375"
     CDS             1394..2317
                     /locus_tag="ACZPAQ_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR0903648.1"
                     /translation="MELTALAGNAQIKAQLAHREGDLAHAYIVAGPAGSGRHTLAAQM
                     AAALVCSAQPQLRPCGRCSHCRKAAGGIHPDITVIAGSGGKPITVDQVRALRTDAYVR
                     PNEAERKVYLLERADRMNASAQNAMLKLLEDGPAYAVFFLLAENGSALLPTVRSRCET
                     LELAPVSPAQAEEWLAKAFPGRTAEEIRRAALECQGILGRAAALLEGGQEEDGLEETA
                     RSLSAALEGEDELALFEATQALDKLSREELTAVLDRTLIQIGARLPGSQRPRRLLAAA
                     ELLRRLRDGADLNANPGQVAGWLCAGMFLRP"
     gene            2382..>2673
                     /locus_tag="ACZPAQ_06380"
     CDS             2382..>2673
                     /locus_tag="ACZPAQ_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 C-terminal domain-containing protein"
                     /protein_id="HIR0903649.1"
                     /translation="MVEIIGVRFKSGGKQYYFDPAGLEVQPGQGVIVETSRGLEYGEC
                     AQGNTQVEEETVVQPLRPLVRIATEEDEKTVEKNREKEAKAFQICQEKIAAHG"
BASE COUNT          470 a          882 c          889 g          432 t
ORIGIN      
        1 tgaaatcccg ccgcgccttc ttacaccgga aaggagacca gtcatgccca caccgctcta
       61 tgatgccctg agaggatacg ccgcaaaagc tcccctccgc ttccatatgc ccggccacaa
      121 gggagccttt cttccctgcc ccgagctggc ccccctggcg cggctggatg tgacagagat
      181 cccgtctaca gggaatctgt atgaggatct ctctcctatc cgggaggccc aggccctgtg
      241 ggcccgccgc atgggagcgg agtactgcca gtttctcacc ggcggctcca ccatgggtat
      301 ccataccgct ctgaccctgt gctgtccccc ggggagccgg gtgctggtgg accggagctg
      361 ccaccgctcc accttccacg ccctggctct tctggatctg gacccgatat ttcttccacg
      421 cccctggctg gcggaagagg gggtggccgg cccgttttcc cccctggatg tgaaaaaaat
      481 gctggatcaa gatccaacta tcaaaactgt ttgtattaca tcaccaactt attgcggatt
      541 gttgtcggat attcccgcca ttgcgggagt agtccacgcc cacggtggaa aactggtggt
      601 ggacggtgcc cacggagccc atctgcaatt tttggggatg gatgcctacc ggggagcgga
      661 cgcggtggtg gtgagcgccc acaagaccct gcccgccatg gggcaggcgg ccctgctgtt
      721 caccaacggc atcgacccgg gacaggtgcg gcgaactgcc gccatctacg gcacctccag
      781 cccctcctac cccatcctgg cctccctgga cacggcccgt gagtggctgg agggaccggg
      841 tgccggagag tatgggcggg tggcccggcg gaccgccgcc ctgcggtgcc ggttcccctc
      901 cctgtgccct cccctgcccc tggaccccac ccgctttact ctgaaggtga agaacgggcc
      961 ggccttcacc gggcagctgg agagccgatg caatatctgg ccggagatgg aggacggggg
     1021 ccatgtggtc tttatcctca ccgcctgcga cggggaagag gctctggacc gcctggagtc
     1081 cgtcctggaa gggatggagg agcagatggg ggacagtgcg cccctgccgc cccctcccct
     1141 gccggagcgg gtgctctccc cccggcaggc ccagttcgcc ccagcggagg tccttcctct
     1201 ggagcggaca gaggggcggg tatgcacctg ccagctggcc ccctatcccc caggtgtgcc
     1261 ggtggtggcc cccggggaac gaattgggaa aaaagagctg gcctatttca gacaaatagg
     1321 ctataataat cgggaagtaa ccgtcgcggc catgccatga aaagggggcg gcggcgcaag
     1381 tgaggaagcg accatggaat tgacagccct ggcgggcaat gcccagatca aggcccagct
     1441 ggcccaccgg gagggggacc tggcccacgc ctatatcgtc gccggcccgg cgggcagcgg
     1501 ccggcatacc ctggccgccc agatggcggc ggccctggtg tgctccgccc agcctcagct
     1561 ccggccctgc ggccgttgtt cccattgcag aaaggccgcc gggggcatcc atcccgacat
     1621 caccgtgatc gctggcagcg gcggaaaacc cattaccgtg gatcaggtcc gtgccctgcg
     1681 caccgacgcc tatgtccgtc caaacgaggc ggagcggaag gtgtacctgc tggagcgggc
     1741 agaccggatg aacgccagcg cccagaacgc catgctcaag ctgctggagg acggccccgc
     1801 ctatgcggtc tttttccttc tggcggagaa cggctccgcc ctgctgccca cggtgcgctc
     1861 ccgatgtgag accctggagc tggcccccgt ctccccggct caggcggagg agtggctggc
     1921 caaggcattt cccgggagaa cggcggagga aatccgccgg gccgctctgg agtgccaggg
     1981 catcctgggc cgggccgcgg cgctgctgga gggcggtcag gaggaggacg gactggagga
     2041 gaccgcccgc tccctgtccg ccgctctgga gggggaagat gagctggccc tcttcgaggc
     2101 cacccaggcc ctggacaaac tgagccggga ggagcttacc gctgtactgg accggacatt
     2161 gatccagatc ggggcccgcc tgcccggctc ccagcggccc cgccgtctgc tggcggccgc
     2221 cgagctgctg cgccgcctgc gggacggggc ggacctgaac gccaacccgg gccaggtggc
     2281 cggatggctg tgcgcaggga tgtttctgcg cccgtgactc cctcgcgccg gggtgccgtt
     2341 cccaccccgt gcgatcccca atatagcaac aggaggacct tatggtagag atcatcggcg
     2401 tccgcttcaa aagcggcgga aagcaatatt attttgaccc cgccgggctg gaggtgcagc
     2461 ctggacaggg agtgatcgtg gagacctccc ggggcctgga atacggagag tgtgctcagg
     2521 ggaacaccca ggtggaggag gagactgtgg tccagcccct gcgcccactg gtacgcatcg
     2581 ccacggagga agacgagaag acggtggaaa aaaaccggga gaaggaggca aaggccttcc
     2641 agatctgcca ggagaagatc gccgcccacg gcc
//
LOCUS       DBMDSJ010000241         2660 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9211_length_2660_cov_1.579523, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000241 DBMDSJ010000000
VERSION     DBMDSJ010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9211_length_2660_cov_1.579523"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="ACZPAQ_06385"
     CDS             <1..644
                     /locus_tag="ACZPAQ_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR0903650.1"
                     /translation="YVNVLMGTKGILRSCISPAAPVAKCVRVTYDTTVLFDEMSNGGG
                     IACDIENAVRMNADCMAVQTFIGAPGESRSLELLCRTADAGARYGIPTLGVVAVGKQM
                     ERTEKFFQLATRVLAENGANVVKSYYCDGFERVAAACPVPIVIAGGKKLPELEALEMA
                     YRSIAEGARGVDMGRNIFQSDCPAGMAQAIGKVVHGGYTAQQAFEVYQEIKNA"
     gene            661..984
                     /locus_tag="ACZPAQ_06390"
     CDS             661..984
                     /locus_tag="ACZPAQ_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903651.1"
                     /translation="MSAEYMHIGIPITNKKPGMTYNEGAKFWVSNVDDYDYKIEYLKF
                     EEGAPFPEVLHRCPHVAYKVDSLEKYIADADSVICGPMQANDQGDQLAFVWKDGAILE
                     LYQPK"
     gene            1314..2273
                     /locus_tag="ACZPAQ_06395"
     CDS             1314..2273
                     /locus_tag="ACZPAQ_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0903652.1"
                     /translation="MDIAQLADRLTAETAKIIYGKETQIRLIVMALLSQGHVLLEDLP
                     GVGKTTLIKTLAAVSGCRSKRIQFVPDLLPADILGMNIYDRNRGEFRRLPGPVFTNLL
                     LADEINRAVPRTQSALLEAMEERQVTIDGETLPLPTPFVVMATQNPVESESTFRLPAA
                     QMDRFLIRLSMGYPAPEEERRMLFSLGDSIPLDGLETVTSPEELVAAQDSLSQIRVSP
                     EVAGYLVSLVGATRSHPMVRMGASPRASRALFRAARAWAAMAGRSFVTPDDIQALIHP
                     VLAHRLLLTNEARLSGVTADGVLDQIAAQLPVPPAAGDEFHGA"
     gene            2263..>2660
                     /locus_tag="ACZPAQ_06400"
     CDS             2263..>2660
                     /locus_tag="ACZPAQ_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903653.1"
                     /translation="MERKALFGRGSSLLISSPALVLFGAGVLLALRLGSGGVAGVCLF
                     FLLLGLAARWWGSRAIRGVSIQVECPRPRLFPGQQTQLIYHLENGKSLPLIWLELSQN
                     APEEGCLTPDDAFESYLQPAPDQEGGAPVPA"
BASE COUNT          438 a          937 c          836 g          449 t
ORIGIN      
        1 cctatgtgaa cgtgctcatg ggcaccaagg gcatcctccg ctcctgcatt tcccccgccg
       61 ccccggtggc caagtgcgtc cgggtgacct atgacaccac cgtcctcttc gatgagatgt
      121 ccaatggcgg cggcatcgcc tgcgacatcg agaacgcggt ccggatgaac gcggactgta
      181 tggctgtcca gaccttcatc ggcgcccccg gcgagtcccg ctccctggag ctgctgtgcc
      241 gcaccgccga tgccggcgcc cggtacggta tccccactct gggtgtagtg gcggtgggca
      301 aacagatgga gcgcaccgaa aagttcttcc agctggccac ccgtgtgctg gcggagaacg
      361 gggccaacgt ggtcaagagc tattactgcg acggcttcga gcgggtggcc gccgcctgcc
      421 cggtgcccat cgtcatcgcc ggcggcaaga agctgcctga gctggaggcc ctggagatgg
      481 cctaccggtc cattgcggag ggggcccgcg gggtggacat gggccggaac atcttccagt
      541 ccgactgccc cgccggcatg gcccaggcca tcggcaaggt ggtccacggg ggctacaccg
      601 cccagcaggc ctttgaggtc taccaggaaa tcaaaaacgc ctgatcagga gggaactttc
      661 atgtccgctg agtatatgca cattggtatc cccatcacca acaaaaagcc cggcatgacc
      721 tacaacgagg gggccaagtt ctgggtgagc aacgtggacg actacgacta caagatcgag
      781 tatctgaaat ttgaggaggg cgcccccttc cccgaggtgc ttcaccgctg cccccacgtg
      841 gcctacaagg tggacagcct ggagaagtac attgccgacg cggacagcgt catctgcggc
      901 cccatgcagg ccaacgacca gggcgaccag ctggccttcg tgtggaagga cggggccatc
      961 ctggagctgt atcagcccaa gtagaaccat actctcaccc acacgccaag agcggggcgc
     1021 tccttcggga gcgccccgct ctgctgtctg cccctccggg gaacttcgcc agccgtcccc
     1081 gtccggcttg ctgaggatgg ctcccgcccc ctgacagggc agatatttca gaaagcggcg
     1141 caccgccatg cgggcgctct tccggctccg gaaggcccgg gcactgtccg cccagccctt
     1201 cggggcgggg tcgggcactt ctttacaaat tggggataga attcccaaaa aatcgtggta
     1261 aaatggagct tatctgtaaa accggcgaga gccggcacaa ggaggagctc cctatggata
     1321 tcgctcagct ggcagaccgc ctgactgcgg agaccgccaa gatcatctac ggcaaggaga
     1381 cgcagatccg cctgatcgtc atggcactgc tctcccaggg ccacgtgctt ctggaggacc
     1441 tgcccggcgt aggcaagacc accctgatca agaccctggc cgccgtgtcc ggctgccgat
     1501 ccaagcgcat ccagttcgtc cccgacctgc tgcccgccga catcctgggc atgaacatct
     1561 acgaccggaa ccggggggag ttccgccgcc tgcccggccc cgtgttcacc aatctgctgc
     1621 tggccgacga gatcaaccgg gcggtccccc gcactcagtc cgccctgctg gaggccatgg
     1681 aagagcggca ggtcaccatc gacggcgaga ctcttcccct ccccaccccc tttgtggtga
     1741 tggccaccca gaacccggtg gagtcggaga gcaccttccg cctccccgcc gcccagatgg
     1801 accgctttct catccgcctc tccatgggct accccgcccc tgaggaggag cggcggatgc
     1861 tcttctccct gggagacagc atcccgctgg acggtctgga gactgtcacc tccccggagg
     1921 agctggtggc cgcccaggac tccctctccc agatccgggt gtccccggag gtggccggct
     1981 acttggtttc cttggtgggg gccacccgca gccaccccat ggtccggatg ggggccagcc
     2041 cccgggcctc ccgggccctg ttccgggccg cccgggcctg ggccgccatg gcgggccgct
     2101 ccttcgttac cccggacgac atccaggccc tgatccaccc cgtgctggcc caccggctgc
     2161 tgctcaccaa cgaggcccgg ctctccggtg tcaccgctga cggggtgctg gaccagatcg
     2221 ccgcccagct ccccgtcccc ccggcggcgg gagatgagtt ccatggagcg taaggccctg
     2281 ttcggccggg gctccagcct gctgatctct tcccctgccc tggtgctgtt cggggccggg
     2341 gtcctgctgg ccctgcgcct gggcagcggc ggggtggcgg gggtatgcct gttcttcctc
     2401 ctgctggggc tggccgcccg gtggtggggg agcagagcca tacgcggcgt ctccatccag
     2461 gtggagtgcc cccgcccccg gctctttccc gggcagcaga cccagctcat ctaccatctg
     2521 gagaacggga agtctctgcc gctgatctgg ctggagctgt cccaaaacgc cccggaggag
     2581 gggtgcctca ccccggacga tgcctttgag tcctatctgc agcccgcccc ggatcaggaa
     2641 gggggcgccc cggtccccgc
//
LOCUS       DBMDSJ010000242         2660 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9212_length_2660_cov_1.547089, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000242 DBMDSJ010000000
VERSION     DBMDSJ010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_2660_cov_1.547089"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(22..837)
                     /locus_tag="ACZPAQ_06405"
     CDS             complement(22..837)
                     /locus_tag="ACZPAQ_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR0903654.1"
                     /translation="MGMKTSAVWADGTFESNTAPLMGPLEDCARRAAELGYDALSLTV
                     NRPGELDAAYVRRVLGEYGLTASGLATGRIYTVDGLGLGLADQERRQAAVDRMLSHAE
                     LCAQLGGAKLIVGAIRGWTRDAGGREAYEPLFRSSMESILARAEQLGIQVAFEAISRI
                     DSDAYCSLSETAEFIRSFRSPALRLQVDSIHLHTNGETDFYQGLLKAGDLIGQVDISD
                     VDRMAPDGRHFDFPLLIQALKETGFQDYLVFEFRANPPADAAKAGLDYIRALL"
     gene            complement(1236..1772)
                     /locus_tag="ACZPAQ_06410"
     CDS             complement(1236..1772)
                     /locus_tag="ACZPAQ_06410"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR0903655.1"
                     /translation="MRRTVLYISMSLDGCLARPDGDVSWLAGQDPDDPDPGTYPAFLS
                     TVDTVVMGWTTYHQVVTQISPGSWPYRGLDCYVLTHRPLPPQTEKIQAFSGPAHQLVE
                     QLKREPAGRDIWICGGADTAAQLIRKNAIDRYVVSVIPVILGEGIPLFSRGLPQQDLR
                     LVSAAHSNGIAELTYEPR"
     gene            complement(1772..2269)
                     /gene="rimM"
                     /locus_tag="ACZPAQ_06415"
     CDS             complement(1772..2269)
                     /gene="rimM"
                     /locus_tag="ACZPAQ_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980137.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR0903656.1"
                     /translation="MKEQYLEVGKITNVHGIMGEVRVQPWADSPDFLCQFKTLYVDSS
                     HWPIQVERARVHKNMVILKLQGVTDVNSALAMRNAVLYIDRKDVQLPEGSFFLADLMG
                     MEVRDAASGAVLGQIADILTLPANNVYVVRGGARELMIPAVDQFIAEVNVDEGYLRVN
                     MMEGL"
     gene            complement(2331..2561)
                     /locus_tag="ACZPAQ_06420"
     CDS             complement(2331..2561)
                     /locus_tag="ACZPAQ_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR0903657.1"
                     /translation="MKELLTYVAQNLVDHPDQVSVTQHESDGETVLELRVAPEDMGKV
                     IGRQGRIAKEIRTLIRSVAQRTGQRVSVEIVD"
BASE COUNT          457 a          847 c          901 g          455 t
ORIGIN      
        1 agcggaacaa actgggcccg ttcacagcag agcgcggatg tagtccagcc cggccttggc
       61 cgcgtcggca gggggattgg cacggaactc aaagaccaga tagtcctgga atccggtctc
      121 cttcagggcc tggatcaaca gcggaaaatc aaagtgcctg ccgtctgggg ccatgcggtc
      181 cacatcggag atgtccacct gtccgatgag gtcgcccgcc ttcagcagcc cttggtagaa
      241 atccgtctcc ccgttggtgt ggagatggat ggagtccacc tggagccgga gggcagggga
      301 gcggaaggag cggatgaact ccgcggtctc ggacagggag cagtaggcgt cggagtcgat
      361 gcggctgatg gcctcgaagg ccacctggat gcccagctgt tccgcgcggg ccaggatgga
      421 ctccatgctg gagcggaaca gaggctcata ggcctcgcgg ccgccggcat cccgggtcca
      481 gccccggatg gcgcctacga tcagcttggc gccccccagc tgggcacaca gctccgcgtg
      541 ggacagcatc cggtccaccg cggcctggcg ccgctcctgg tccgccaggc ccaggcccag
      601 gccgtctacg gtgtagatcc gtcccgtagc cagtcccgat gcggtcaggc cgtactctcc
      661 cagcacgcgg cggacatagg cggcatccag ctcgccgggg cggttgaccg tcagggagag
      721 ggcgtcatag cccagctccg ccgcccggcg ggcgcagtcc tccaaaggcc ccatcagggg
      781 ggcggtgttg ctctcaaagg tgccgtctgc ccagacggcg ctggtcttca ttcccatagg
      841 tatctcctgt tccgggggca agtccccctg catgtgtgat ggtttattat agcaccgggg
      901 ccggccgggg tcaattcccg gccgggaaag agagcggctc cgcgggagcg gacagggcct
      961 gatgccccca tcgagccgga cggcgggcgc acgatgtgcg cccctgcgca aaagctgaag
     1021 atatctcgca ggggcagccc ccggagccgc cctcaaagcc ttcccccctg ggtggggaag
     1081 gtgtcacggc ggacgccgtg acggatgagg ggggtgtccc ctgccggtcc aatcaacact
     1141 ccgctgagtc cgatcagggg cggggcggct ccccgcccct gtgcaggaaa cggaaaaccc
     1201 cgcctgcgtt tcatacaggc ggggctagcg gcgggtcagc ggggttcata ggtcagctcg
     1261 gcgatcccgt tgctgtgggc ggcggagacc agacgcaggt cctgctgggg cagcccgcgg
     1321 gaaaagaggg ggatgccctc ccccaggatc acggggatca cagagaccac atatcggtcg
     1381 atggcgtttt tccggatcag ctgggcggcc gtgtcggcgc cgccgcagat ccagatgtcc
     1441 cgcccggcgg gctcccgctt cagctgttcc accaactggt gggccggacc gctgaaagcc
     1501 tggatcttct ccgtctgggg agggaggggg cggtgggtga gcacatagca gtccaatccc
     1561 cggtagggcc agctgccggg agagatctgg gtgaccacct ggtggtaggt ggtccagccc
     1621 atgaccacgg tgtccactgt ggagagaaag gcggggtagg tccccgggtc cgggtcatca
     1681 gggtcctggc cggccagcca ggagacatcc ccgtccgggc gggccaggca gccgtccagg
     1741 ctcatggaga tgtagaggac ggtgcggcgc atcacaggcc ctccatcatg ttgacccgga
     1801 ggtatccctc gtccacgttg acctccgcga tgaactggtc caccgcgggg atcatcagct
     1861 cccgggcgcc gccgcggact acatacacat tgttggcggg cagggtgagg atgtccgcga
     1921 tctgtcccag caccgcgccg ctggcggcgt cccggacctc cattcccatc aggtcagcca
     1981 ggaaaaagct gccctccggg agctggacat ccttccggtc gatgtacagc acggcgttgc
     2041 gcatggccag agcactgttg acatcggtga cgccctgcag cttcaggatg accatgtttt
     2101 tgtggacccg ggcccgctcc acctggatgg gccagtggga gctgtccaca tagagggtct
     2161 tgaactggca gagaaagtcc ggggagtccg cccagggctg tacccgcacc tcgcccataa
     2221 tgccgtggac gttggtgatc tttccgacct ccagatactg ttctttcatg gggattctcc
     2281 tttcctccgg ggtaagacaa tggcgcagga tctccctgcg ccataactgt ctagtcgacg
     2341 atctcgacgg agacacgctg accggtgcgc tgggccacgg agcggatcag ggtgcggatc
     2401 tccttggcga tgcggccctg acggccgatc accttcccca tatcctccgg agccacacgg
     2461 agctccagca cggtctcgcc gtcgctctca tgctgggtga cagacacctg atcggggtgg
     2521 tccaccagat tctgggcgac ataggtcaaa agctctttca tttgcctgac agacctccag
     2581 ccttacagcg cgccggcctt tttcagaagg gagcggacgg tgtcggtggg ctgagcaccg
     2641 gtcttgatcc aggcctgagc
//
LOCUS       DBMDSJ010000243         2657 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9222_length_2657_cov_1.730438, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000243 DBMDSJ010000000
VERSION     DBMDSJ010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9222_length_2657_cov_1.730438"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="ACZPAQ_06425"
     CDS             complement(<1..621)
                     /locus_tag="ACZPAQ_06425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0903658.1"
                     /translation="MYRIAFFSIYAHGHTNPTLPVVRELTCRGHQVRYYSFAPFREKI
                     QAAGAQFVPCDSYLPPAPPDLERRVGRDFAALVEMITQTLLAMDGPVGRDLAQFQPHC
                     VVSDSLCFWGKLWAAKLACPYVCSTTTFAFNRHTAKLMKQTPGQFLRLLAGRGRIRRC
                     MAQLCQAGYPVKGLLSLIENDGHTYTIVYTSREFQPLADTFSSRYAF"
     gene            complement(649..945)
                     /locus_tag="ACZPAQ_06430"
     CDS             complement(649..945)
                     /locus_tag="ACZPAQ_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR0903659.1"
                     /translation="MSDNEKYMSSVKVGPKGQIVIPKEIRDMFQISPGDTLLILADAQ
                     RGIALERYQVFEQIAESIFQGRGRDIYPDHSESESRAFAQAIRQLSTPEEPEER"
     gene            1146..1913
                     /gene="aroD"
                     /locus_tag="ACZPAQ_06435"
     CDS             1146..1913
                     /gene="aroD"
                     /locus_tag="ACZPAQ_06435"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR0903660.1"
                     /translation="MKTKTVLLRGVEIGAGRPKIIVPIVGKTEQEILDKAAELVQAKP
                     DVVEWRVDYFQGVGDVQRVVDTARELNEALGEIPILFTFRTKREGGEKEIGMEDYTAL
                     NRAVAQSSYVDAVDVEIFSGDEVVRKNIAAIHDAGAAVVGSSHEFHRTPEHAELIYRL
                     RKMQDMGADILKVAVMPQNKADVLTLLSATEEMYRLYAQRPLLTMSMASDGVISRLCG
                     EVFGSAATFGMVGQGSAPGQIPADQLEQVLEILHRAL"
     gene            2222..2632
                     /locus_tag="ACZPAQ_06440"
     CDS             2222..2632
                     /locus_tag="ACZPAQ_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903661.1"
                     /translation="MKKELIALGAALALVGTLTACSSTPADQNKGSAGSGATGSTTGS
                     AAGTAYPRSTRYGMNGTAGGHGYTYDGRYTAFPDGRVAPGRGNAARDMERVGKGVADG
                     TRDLVNGVGDVVRDAGNGLRDAGQAVKNAADRDM"
BASE COUNT          546 a          754 c          908 g          449 t
ORIGIN      
        1 aaaggcatac cggctggaaa aggtgtcggc caggggctgg aattcccggg aagtgtagac
       61 gatggtgtag gtgtgtccgt cgttctcaat gagggagagc agtcccttca ccggatagcc
      121 cgcctggcac agctgggcca tgcagcggcg gatgcggccc cgacccgcaa gcagccgcag
      181 gaactgcccc ggcgtctgct tcatcagctt ggcggtatgg cggttgaagg cgaaggtggt
      241 ggtggagcag acataggggc aggccagctt ggcggcccac agctttcccc agaagcacaa
      301 ggagtcggat accacgcagt ggggctggaa ttgggccaga tcccggccca cggggccatc
      361 catggccagg agggtttggg tgatcatctc caccagggcg gcgaagtcgc ggcccaccct
      421 tcgctccaga tccggaggcg ccggaggcag ataggagtcg caggggacga attgggcgcc
      481 ggcggcctgg atcttctccc gaaagggggc gaaggagtaa tagcggacct ggtgcccacg
      541 gcaggtcagc tcccgtacca ccggaagagt gggattggtg tgcccgtgag catagatgga
      601 gaaaaaggcg atgcggtaca tgaagtcccc tcctttccct ggcagagctt accgttcctc
      661 aggctcttcc ggtgtgctca gctgccggat ggcctgggca aaagccctgg actccgactc
      721 gctgtggtca ggatagatat cccggccccg cccctggaag atggactcgg cgatctgctc
      781 aaagacctgg tagcgctcca gggcgatgcc ccgttgtgcg tcggccagga tcagcagggt
      841 gtccccgggg ctgatctgga acatgtcccg gatctcctta ggaatgacga tttggccttt
      901 cggtcccacc ttgacggagc tcatatactt ctcattgtca gacatcttga tccctaaatc
      961 tccttaaagt aatacttgta taactaatct taccttcgaa aggcggaaat gtcaagaaaa
     1021 atctgtcccc tctcccgcgc gggggtggac ggccgcctgg cgctctgcta taatgcacct
     1081 gttgatccaa aagagcggcc cccggcgggg ctggccgatt cagaagaagg agcgggtgag
     1141 aactgatgaa gacaaagacc gtactgttgc gcggggtgga gatcggtgca ggcagaccga
     1201 agatcatcgt ccccattgtt gggaagacgg agcaggagat cctggacaaa gccgccgagc
     1261 tggtccaggc caagcccgat gtggtggaat ggcgggtgga ctacttccag ggcgtgggcg
     1321 atgtccagcg ggtggtggat actgccagag agctgaacga ggcactgggg gagatcccca
     1381 tcctcttcac cttccgcacc aagcgggagg gcggcgaaaa ggagatcggt atggaggact
     1441 acaccgcttt gaaccgggcc gtcgcccagt cgagctatgt ggacgcggtg gatgtggaga
     1501 ttttttccgg tgacgaggtg gtgcgcaaga acatcgccgc catccatgat gccggcgcgg
     1561 cggtggtggg gtccagccat gaattccacc ggacaccgga gcacgccgag ctcatctacc
     1621 ggctgcggaa gatgcaggat atgggggccg acatcctgaa ggtggctgtg atgccccaga
     1681 acaaggcgga cgtgctgacc ctgctctccg ccaccgagga gatgtatcgg ctgtacgccc
     1741 agcggccgct gctgaccatg tccatggcgt cggacggcgt catcagccgc ctgtgcggcg
     1801 aggtgttcgg ctccgccgcc accttcggca tggtgggcca aggctccgcc cccggccaga
     1861 tcccggcgga tcagctggaa caggtgctgg agatcctcca cagagcgctg tgaggtttga
     1921 ccggcccgcc tgtctccagg cgggccggtg attttgccag ggaaaatgga ctcaccagca
     1981 taacggacga gctgcgctgc cccataatgc tgctgatctg tgcaaaaaaa ttgtaaaaca
     2041 cagccatgtc tgccccgatt taccggcagt aaagggcaac gctgtgggca caataaagct
     2101 cggatggaac ttccgtatga gaaaagtgtg ggatcaggtc ccgtctggaa cgtgtgccag
     2161 gcgggacctg acagccccgg ccggaccggc cgggctggaa cggaaaacgg gaggatttgg
     2221 aatgaaaaaa gagctgatcg ccctgggggc ggctctggcc ctggtgggga ccctgaccgc
     2281 gtgcagcagc acgccggcgg accagaataa ggggagcgcc ggcagcggcg ctaccggcag
     2341 tactaccggc agcgccgccg ggactgccta tccccggtct acccgctacg ggatgaacgg
     2401 gaccgctggc ggccatggct acacctatga cggccgctat accgccttcc ccgacggcag
     2461 agtggcaccg ggccggggca atgcggcccg ggacatggag cgagtgggaa aaggcgtggc
     2521 tgacgggacc cgggacttgg tgaacggcgt gggcgacgtg gtgcgcgacg ccgggaacgg
     2581 tctgcgggat gccggccagg cagtgaaaaa cgcagctgac cgggacatgt gacccataaa
     2641 acgctgagaa aagaccg
//
LOCUS       DBMDSJ010000244         2641 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9296_length_2641_cov_1.989764, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000244 DBMDSJ010000000
VERSION     DBMDSJ010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9296_length_2641_cov_1.989764"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /gene="rlmD"
                     /locus_tag="ACZPAQ_06445"
     CDS             complement(<1..1366)
                     /gene="rlmD"
                     /locus_tag="ACZPAQ_06445"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR0903662.1"
                     /translation="MAEVRKNTVHPLHIEGYTAEGMGVARLDGRVVFIPGTIRGEDWE
                     VQLLKVNKGVAWGKGTRLISPSPVRQEPDCPYSGRCGGCQYRHMSYEEELGAKGQRVR
                     DALERVGGVHLELPPVLGAESPLRYRNKVQFPVSRGKCGLSIGFYRPRSHDVVDTEDC
                     LLQPEADTALRLAFKGWMEDFQIPAYDESTGRGLIRHLYIRTNRAGEALCCVVANGRE
                     LPHAGELSGALRAAVPKLAGLVLNVNLRDTNVILGDAYRTLWGRDWLEEELCGLTFRL
                     SVPSFFQINRDQTERLYQLALEFAGLTGEETVLDLYCGIGTISLCLARRAGHVIGAEV
                     VPQAIEDARANALRNGVTNAEFFCGDAGEVAARLAGQGVRPQVICVDPPRKGLAPEVP
                     AVLASMAPDHIVYVSCDPATLARDVKRLEELGYRAVQAQPVDLFPRTAHVETVCLLSK
                     LQSKE"
     gene            complement(1510..2337)
                     /locus_tag="ACZPAQ_06450"
     CDS             complement(1510..2337)
                     /locus_tag="ACZPAQ_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024748.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR0903663.1"
                     /translation="MKRIPQNEIYRILSGLAGTVGLYVEDCQSGEIFTINPEHVFPSA
                     SVIKVPMLALLMRDAQQGLVDLDAPRTIDPRNRVGGTGILCELGTDYTPSLRDLAKLM
                     IVLSDNIATNEIMDVIGMERFNSFCREMGYQNITLMRKMMDFEAIQQGKNNYMCAGET
                     GRLLSAISRGEFVNPEISQTIFDIMASQQCRNKLPALLPAVASYSGSKSAIPEGQVLV
                     ANKTGDLVGIQHDVGIFELPDGRRYLIAMFTGDLENDSAGIAAISQVSLAVYQALRD"
     gene            complement(2373..>2641)
                     /locus_tag="ACZPAQ_06455"
     CDS             complement(2373..>2641)
                     /locus_tag="ACZPAQ_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR0903664.1"
                     /translation="INGAAVAQAMMIDSLFSSFLPTLGISMELWGMIVLIGHQITSFA
                     YPGVDMIGQMGLAHASNIKPMMKLSYFAIIPGTVILAVLASFIL"
BASE COUNT          491 a          847 c          835 g          468 t
ORIGIN      
        1 gttccttgct ttgaagtttg gacaacaaac acaccgtctc cacatgggcg gtcctgggaa
       61 acagatccac cggctgggcc tggacggccc ggtagcccag ctcctccagc cgtttcacat
      121 cccgggccag ggtggccggg tcgcaggaga cgtagacgat gtggtccggc gccatggagg
      181 ccagcaccgc cggcacctcc ggggcgagcc ctttgcgggg cgggtccacg cagatgacct
      241 ggggacgcac gccctggccg gccaggcggg ccgccacctc ccccgcgtcc ccgcagaaga
      301 attccgcgtt ggtgacaccg ttgcgcaggg cgttggccct ggcgtcctcg atggcctggg
      361 ggaccacctc cgccccgatg acatgcccgg cccggcgggc caggcacagg gagatggtgc
      421 cgatgccgca gtacaggtcc agcaccgtct cctccccggt gagcccggcg aattccagcg
      481 ccagctggta cagccgctct gtctgatccc ggttgatctg gaagaaggag ggaacggaca
      541 ggcggaaagt gagcccgcac agctcctcct ccagccagtc ccggccccag agggtgcggt
      601 acgcatcgcc caggatcaca ttggtgtccc ggagattcac attgaggacc agcccggcca
      661 gcttggggac agcggcgcgc agggcaccgg acagctctcc ggcgtggggc agctccctcc
      721 cgttggccac tacgcagcac agggcctccc ccgcccggtt ggtacggata tacaggtgcc
      781 ggatcagccc ccggccggtg gactcatcat aggcggggat ctgaaagtcc tccatccagc
      841 ccttgaaggc cagacgcagg gccgtatccg cctccggctg gagcaggcag tcctccgtgt
      901 ccaccacgtc atggctcctg gggcggtaaa agccgatgga cagcccgcac tttcctcggg
      961 agacagggaa ctgcaccttg ttgcggtagc gcagggggga ctccgccccc agcaccggag
     1021 gcagttccag atggacgccg cccacccgct ccagggcgtc ccgcacccgc tggcccttgg
     1081 cccccagctc ctcctcatag gacatgtggc ggtactggca ccccccgcac cgcccgctgt
     1141 aagggcagtc cggctcctgc cggacggggg agggggagat cagacgtgtc cctttgcccc
     1201 aggccacccc cttgttcacc ttgagcagct ggacctccca gtcctccccc cggatagtcc
     1261 cgggaatgaa gaccacccgg ccgtccagac gggccacccc cattccttcc gcggtatagc
     1321 cctcgatatg gaggggatgc actgtgtttt ttctgacctc ggccatggga ttccctcgct
     1381 tcaaaaccgt ttttcttatc atatcatggc cggatggagc cgtaaagccc attttgcctc
     1441 aggacggcag agagaacagg agcgccgccc gaaagggcgg cgctccgcat agggcgcaga
     1501 gggcggcggt cagtcccgaa gggcctgata caccgccagg gagacctggg agatggcggc
     1561 gatgcccgcg ctgtcgttct ccagatcccc ggtgaacatg gcaatcaggt agcggcggcc
     1621 gtcaggcagc tcaaagatgc ccacgtcgtg ctggatgccc accaggtcgc ctgtcttgtt
     1681 ggccaccagg acctgccctt ccgggatggc ggacttggag ccggagtagg aggcaactgc
     1741 cggcagaagg gcgggcagct tgttgcggca ctgctgagat gccatgatat cgaagatggt
     1801 ctgggagatc tccggattga cgaattcgcc ccgggagata gcagaaagca gacgcccagt
     1861 ctccccggcg cacatgtagt tgttctttcc ctgctggatg gcttcaaaat ccatcatttt
     1921 ccgcatcagg gtaatattct gatagcccat ctcccggcag aagctgttga accgctccat
     1981 tccgatcaca tccatgatct cattggtggc gatgttgtcg ctgagtacga tcatcagctt
     2041 ggccagatcc cgcagggaag gggtgtaatc ggtgcccagc tcgcacagga tgccggtccc
     2101 gcccacccgg ttgcgggggt cgatggtgcg gggggcgtcc aggtccacca gcccctgctg
     2161 tgcgtcccgc atgagaaggg ccagcatagg gaccttgatg acgctggccg agggaaacac
     2221 atgctccggg ttgatggtga agatctcgcc ggactggcag tcctccacat agaggcccac
     2281 cgtcccggcc agtccggaga ggatgcgata gatttcattt tgagggatac gtttcatgag
     2341 aatatgcctc ctgtcctatt tccgaaagct agttacagaa tgaacgacgc cagcactgcg
     2401 aggatcactg tgccgggtat aatggcaaag tacgagagct tcatcatcgg cttgatgttg
     2461 ctggcatggg ccagtcccat ctgtccgatc atgtccacgc cgggataggc aaatgaggtg
     2521 atctgatgac cgatgaggac gatcatgccc cacagctcca tggaaatgcc cagggtcggg
     2581 aggaaggagc tgaacaggct gtcgatcatc atggcctggg ccacggcggc accgttgatg
     2641 c
//
LOCUS       DBMDSJ010000245         2639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9302_length_2639_cov_1.795902, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000245 DBMDSJ010000000
VERSION     DBMDSJ010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9302_length_2639_cov_1.795902"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /gene="hisH"
                     /locus_tag="ACZPAQ_06460"
     CDS             complement(<1..579)
                     /gene="hisH"
                     /locus_tag="ACZPAQ_06460"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR0903665.1"
                     /translation="MGTVAIVDYGVGNLKSVANALSYLGCSSCITADPAELERADAII
                     LPGVGAFPDAADRLRSGGLDQHVLAQGQKKPVLGICLGMQLLLEQGREVRPCPGLGVI
                     RGTVDRIPTQSKLPHIGWNSLRFFHHSPLFRGLDEGAYVYFVHSFSAQDTDPAQVIAA
                     TDYGPAVTAAVSSGNFYGTQFHPEKSGEVGLTI"
     gene            complement(579..1190)
                     /gene="hisB"
                     /locus_tag="ACZPAQ_06465"
     CDS             complement(579..1190)
                     /gene="hisB"
                     /locus_tag="ACZPAQ_06465"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR0903666.1"
                     /translation="MEIPVYEAEESPCRAYVIQRRTKETRISVDLCLEGGEIRISTGI
                     GFFDHMLTAFAFYGGLGLKIEAEGDLEVDGHHTVEDTGIVLGQALNRALGDRKGLRRF
                     ASACIPMDEALCFTVLDFSNRPFLVFDADMPQPMIGTYDPCLTEEFLRALAVNSGLTL
                     HMKCLYGKNAHHITEALFKSLGAAVKEAVQITGTGVTSTKGVL"
     gene            complement(1190..2050)
                     /locus_tag="ACZPAQ_06470"
     CDS             complement(1190..2050)
                     /locus_tag="ACZPAQ_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR0903667.1"
                     /translation="MSKRADPHGLRSKHRSALRLCLGTAYYSARRYLLWMSGKYRFAS
                     QRQKDRLPCVHMAHHTPLLRKLKDVEMQYQHNKITNLKLAVARLDGIVVQPGETLSYW
                     KLIGRPTYRKGYLDGVLLKNGTVQYGCGGGLCQLSNLIFWMTLHTPLEVKERHRHGYD
                     VFPDSNRTQPFGSGATCFYPYGDLMIYNPTDRPFQLCLRVGETDLEGEWRSDRPPQFR
                     YQIVERHHEMRHEWWGGCSRHNELYLQVFDLEGELLEDRLVVENHAMMMYTPFLEDNA
                     PDGGREEEEK"
     gene            complement(2092..>2639)
                     /locus_tag="ACZPAQ_06475"
     CDS             complement(2092..>2639)
                     /locus_tag="ACZPAQ_06475"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR0903668.1"
                     /translation="KYVAADLLSQAEHDRLASAVLLTDSWELAQAVDREVVRQTGYLE
                     RSEIMEESLRDFGCAIVCDSLERCVELANEIAPEHLEIMTEEPRALLPLVKNAGAVFL
                     GAYTPEPLGDYLAGPDHVLPTSGTARFFSPLSVDSFLKTMSVLEFDRAALAPIAGEII
                     TLAETEHLTAHANSIRVRMDG"
BASE COUNT          467 a          882 c          795 g          495 t
ORIGIN      
        1 gatggtcagc cccacctcgc cgctcttctc cgggtggaac tgggtcccgt agaagttccc
       61 gctggacacc gccgctgtca ctgccggccc gtaatcggtg gcagcgatca cctgggcggg
      121 atcggtgtct tgggcgctga aggagtggac gaagtagaca tacgccccct cgtccagccc
      181 ccggaacagg ggcgagtggt gaaaaaaccg caggctgttc catccaatgt ggggcagctt
      241 gctttgggtg ggtatccggt ccactgtgcc gcggatcacc cccagaccgg gacaggggcg
      301 cacctcccgc ccctgctcca gcagcagctg catccccagg cagatgccca gcaccggctt
      361 cttctgaccc tgggccagca catgctggtc cagccctccg ctccgcagcc ggtccgccgc
      421 gtccgggaag gcccccactc caggcaggat aatggcgtca gcccgttcca gctccgccgg
      481 gtcggcggtg atgcagctgc tgcagccgag atagctcagg gcgttggcca cactcttcag
      541 attgcccacc ccgtagtcca cgatggcaac tgtgcccatc acagcacccc cttggtggaa
      601 gtgacgccgg taccggtgat ttgaacggcc tccttcaccg ccgctcccag ggacttgaag
      661 agggcctctg tgatgtggtg ggcatttttt ccataaaggc acttcatatg cagagtgagg
      721 ccgctgttca ctgcaagggc ccgcagaaac tcttcggtga ggcagggatc ataagtcccg
      781 atcataggct gaggcatatc agcgtcaaag accaggaagg gccggttaga aaagtccaga
      841 accgtgaagc acagggcctc gtccattggg atacaggcgg aggcgaagcg gcggagcccc
      901 ttccgatccc ccagggcccg gttcagagcc tggccaagga cgatgcccgt gtcctccacc
      961 gtgtggtggc cgtctacctc cagatctccc tcggcctcta ttttcagccc cagtccgccg
     1021 taaaaagcga aggctgtgag catgtggtca aaaaagccta tgccggtgga tatgcggatc
     1081 tcgccgcctt ccaggcacag gtccacgctg atccgggtct ccttggtcct gcgctggatg
     1141 acataggccc ggcaagggct ctcctccgcc tcatatactg ggatttccat tatttttcct
     1201 cctcttcccg ccccccgtcg ggagcattgt cctccaaaaa cggggtgtac atcatcatcg
     1261 cgtggttctc caccaccagc cggtcctcca gcagctctcc ctccagatca aagacctgga
     1321 gatacagctc attgtggcgg ctgcatccgc cccaccactc atggcgcatc tcatgatgac
     1381 gctccacgat ctgataccgg aactggggcg gccggtcgga gcgccactcc ccctccaggt
     1441 ccgtctctcc cacccggagg cacagctgga agggccggtc cgtgggatta tagatcatca
     1501 ggtctccgta gggatagaag caggtggctc cgctcccaaa gggctgggtc cggttggagt
     1561 cgggaaagac gtcgtagcca tggcggtgac gctccttcac ctccagcggg gtgtgcaggg
     1621 tcatccaaaa gatgagattg gacagctgac acagcccccc gccgcatccg tactgcacgg
     1681 tcccgttttt cagcagcacg ccgtccagat accccttccg gtaggtgggg cggccgataa
     1741 gcttccaata gctcagcgtc tcccccggct ggaccacgat cccgtccagc cgggccaccg
     1801 ccagcttcaa attggtgatc ttattgtgct gatactgcat ctccacatcc ttcagctttc
     1861 ggagcagagg ggtgtggtgg gccatatgga cgcagggcag cctgtccttc tgccgctggg
     1921 aggcgaaccg atacttgccg gacatccaca gcaggtaccg ccgcgccgag tagtaggcgg
     1981 tccccaggca caggcgcagg gcggagcggt gcttggaccg caggccgtgg ggatctgccc
     2041 gcttggacat tctcatcccg atctcctttc ccgttccccc tccgggggct gtcagccgtc
     2101 catccgcacc cggatggagt tggcgtgggc agtcagatgc tcggtctctg ccagtgtgat
     2161 gatctccccg gcaatgggcg ccagggcggc tcggtcgaat tccagcacgc tcatggtctt
     2221 caaaaagctg tccacgctca ggggagagaa gaagcgggcg gtgccgctgg tgggcagcac
     2281 atggtccggg ccggccagat agtcccccag gggttccggc gtataggccc ccaggaagac
     2341 cgctccggcg ttcttcacca gaggcagcag ggccctgggc tcctccgtca tgatctcaag
     2401 gtgctccggg gcgatctcgt ttgccagctc cacacagcgc tccaggctgt cacagacgat
     2461 ggcgcacccg aagtcccgca gggattcctc catgatctcc gagcgctcca gatacccggt
     2521 ctgccggacc acctcccggt ccaccgcctg ggccagctcc cagctgtcgg tgagcagcac
     2581 ggcggaggcc agcctgtcgt gctccgcctg gctgagcagg tccgcggcca cgtacttcg
//
LOCUS       DBMDSJ010000246         2639 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9305_length_2639_cov_1.549645, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000246 DBMDSJ010000000
VERSION     DBMDSJ010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_2639_cov_1.549645"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="ACZPAQ_06480"
     CDS             <1..234
                     /locus_tag="ACZPAQ_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR0903669.1"
                     /translation="VFIMFEKVKEVILDTLSCEPEQITMEASLVDDLEADSLDAVELN
                     LALNDVLGFSLTDEELKSIVTVGDIVRLLEEHQ"
     gene            238..669
                     /gene="accB"
                     /locus_tag="ACZPAQ_06485"
     CDS             238..669
                     /gene="accB"
                     /locus_tag="ACZPAQ_06485"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR0903670.1"
                     /translation="MDLNEIKALMDRFDTSACTVLELEEGNLRLRLEKGTAPVSVAPE
                     IQAAPAAPGAAAPQPAPAEEGQTLNAPLVGTFYAAPAPGEAPFVKAGDQVRKGQTVCV
                     LEAMKMMSEVPAPADCIIEEVLVKDGELVGFDAPMFRIREV"
     gene            671..2041
                     /gene="accC"
                     /locus_tag="ACZPAQ_06490"
     CDS             671..2041
                     /gene="accC"
                     /locus_tag="ACZPAQ_06490"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR0903671.1"
                     /translation="MFRRVLIANRGEIAVRIIRACRELEIETVAVYSVADRDALHVQL
                     ATRAVCIGPARAAESYLNQAAILTAAVEKGCDAVHPGYGFLSENPDFADLCAKCGLKF
                     IGPSGDVIRQMGSKAAAKALMRRSGVPVVPGSEGAVDLEEARRVADEVGYPVLVKASA
                     GGGGRGMRRVDRPEDLPALFQAAQSEAVACFGNGELYVEKLILHPRHIEFQILADSQG
                     NVIHLGERDCSIQRKNQKLIEESPSRALTPQLREEMGQAAVRAARAAHYESAGTIEFV
                     LDEDGRFYFIEMNTRIQVEHPVTEFVTGLDLVREQLRIAAGLPLSVGQEEVRLSGCAM
                     ECRINAEDPREGFRPSPGRVEFLHLPGGPGVRVDTALFGGYQMPPYYDSLCAKVVVWA
                     PTRLEAVRRMRRALEELMIQGMVTTAELCHLILYQPDFVKGQYHTGFLEENLETLMAW
                     DCGTEG"
     gene            2157..>2639
                     /locus_tag="ACZPAQ_06495"
     CDS             2157..>2639
                     /locus_tag="ACZPAQ_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005921023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR0903672.1"
                     /translation="MEEIFARRKARLMELRARRKGEDGEPKRLAARPVFDKCPSCGAV
                     VPKTELSHSLYVCTKCGYHHTIGAYLRLAMLLDSKSFRELDQDLVAPNVLGFPGYEEK
                     LERQRDKTGLMEAVVTARGRVEGRSVAAAALDSRFFMGSMGAAVGEKVTRCVEYAQRN
                     R"
BASE COUNT          446 a          790 c          967 g          436 t
ORIGIN      
        1 gtttttatta tgtttgagaa ggtcaaagag gtcattctgg ataccctgag ctgtgagccc
       61 gagcagatca ccatggaagc cagcctggtg gacgacctgg aggccgattc tctggacgcc
      121 gtggagctga atctggcgct gaacgacgtg ctgggcttct ccctcaccga cgaggagctg
      181 aagagcatcg tcactgtggg cgatatcgtc cgtctgctgg aggagcatca gtaagccatg
      241 gatctgaatg agatcaaggc tctgatggac cggttcgaca cctccgcctg taccgtgctg
      301 gagctggagg agggcaacct gcgcctgcgc ctggagaagg ggaccgcccc cgtttcggtt
      361 gccccggaga tccaggccgc cccggcggcc cctggagcgg ctgcgcccca gcccgctccg
      421 gcggaggagg ggcagaccct gaacgccccc ctggtgggca ccttctacgc cgcccccgcc
      481 ccgggggagg ccccctttgt caaagcgggc gaccaggtgc ggaagggaca gaccgtctgt
      541 gtgctggaag ccatgaagat gatgagcgag gtccccgctc cggcggactg catcattgag
      601 gaagtgctgg tgaaggacgg agagctggtg ggctttgacg cccccatgtt ccgcatccgg
      661 gaggtctgaa atgttccgga gggttttgat cgccaaccgg ggcgagatcg cggtgcgaat
      721 catccgggcc tgccgggagc tggagatcga gacagtggcg gtgtactccg tcgccgaccg
      781 ggacgccctc catgtccagt tggccacccg ggcggtgtgc atcggtccgg cccgggcagc
      841 ggaaagctat ctgaaccagg ccgccatcct gacggcggcg gtagagaaag gctgtgacgc
      901 tgtccatccc ggatacggct tcctctctga aaatccggac tttgccgacc tgtgcgccaa
      961 gtgcgggctg aagtttatcg gtccctccgg cgacgtgatc cggcagatgg ggagcaaggc
     1021 ggcggccaag gcgctgatgc gccgctccgg cgtgcccgtg gtcccgggct ccgagggggc
     1081 tgtggacctg gaggaggccc gccgggtggc ggacgaggtg ggctaccccg tgctggtgaa
     1141 ggcctctgcc ggcggcggag gccgcgggat gcgccgggtg gaccggccgg aggacctgcc
     1201 ggccctgttc caggccgccc agtctgaggc agtggcctgc ttcggcaacg gggagctgta
     1261 tgtggagaag ctgatcctcc acccccgcca catcgagttt cagatcctgg ccgactccca
     1321 gggcaatgtg atccatctgg gggagcggga ctgctccatc cagcggaaga atcagaagct
     1381 gatcgaggag tccccctccc gggccctcac cccccagctg cgggaggaga tgggccaggc
     1441 cgccgtccgg gcggcccggg ccgcccatta cgagagcgcg ggtaccatcg aattcgtgct
     1501 ggatgaggac gggcggttct actttattga gatgaacacc cgcatccagg tggagcaccc
     1561 ggtgaccgag ttcgtcacgg ggctggacct ggtgcgggaa cagctgcgca tcgcggcggg
     1621 cctgcccctg agcgtggggc aggaggaggt ccggctgtcc ggctgtgcca tggagtgccg
     1681 catcaacgcg gaggacccca gggagggctt tcggcccagc ccgggacggg tggaatttct
     1741 tcatctcccc ggcgggcccg gggtgcgggt ggacaccgcc ctgttcggcg gctaccagat
     1801 gccgccctac tatgactccc tgtgcgccaa ggtggtggtg tgggccccca cccggctgga
     1861 ggcggtgcgc cggatgcgcc gggcgctgga ggagctgatg atccagggga tggtgaccac
     1921 cgcagaactg tgccatctga tcctctatca gcccgacttt gtaaaggggc agtaccacac
     1981 gggattcttg gaagaaaatc tggagaccct gatggcctgg gactgcggga cggaggggtg
     2041 agtcccgtcc ccgccggatg gtgagatagg aaccttttca gccggaggac ctggctgaac
     2101 ggcacggagc ggagggggag tccctccgct ccggcctgtg cgatggaagg agcgctatgg
     2161 aagagatctt tgcccgccgg aaggcccggc tgatggagct gcgggcccgg cggaaggggg
     2221 aggacggcga gcccaaaagg ctggcggccc ggcctgtgtt cgataagtgc cccagctgcg
     2281 gggcggtggt gcccaagacg gaactgtccc acagcctgta tgtctgtacg aagtgcggat
     2341 accaccacac catcggggcc tacctgcggc tggccatgct gctggactcc aagagcttcc
     2401 gggagctgga ccaggacctg gtggccccca atgtgctggg tttccccgga tatgaggaga
     2461 agctggagcg ccagcgggat aagactggcc tgatggaggc tgtggtcacc gcccggggcc
     2521 gggtggaggg ccgcagcgtg gccgccgccg ccctggacag ccgcttcttt atgggcagca
     2581 tgggagcggc cgtgggggag aaggtgacac gctgcgtgga atatgcccag cggaacagg
//
LOCUS       DBMDSJ010000247         2630 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9339_length_2630_cov_1.611704, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000247 DBMDSJ010000000
VERSION     DBMDSJ010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9339_length_2630_cov_1.611704"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="ACZPAQ_06500"
     CDS             complement(<1..383)
                     /locus_tag="ACZPAQ_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR0903673.1"
                     /translation="MPDTIPNLHQWERGPKITAIGGGTGLSTMLRGLKKYTKNLTAVV
                     TVADDGGGSGVLRRDIGMPPPGDIRHCMESLANVEPIMERLLTYRFQEGSLAGQSFGN
                     LILAALNGVTGSFEEAVAQMSQVLA"
     gene            complement(420..1280)
                     /gene="rapZ"
                     /locus_tag="ACZPAQ_06505"
     CDS             complement(420..1280)
                     /gene="rapZ"
                     /locus_tag="ACZPAQ_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR0903674.1"
                     /translation="MEFIIISGLSGAGKSKAASFMEDMGFFCVDNLPAPLIPKFAELG
                     MAGTGEYDRVVLVTDVRSGTNFSALFQSLEALKGMKCPYRILYMDASDDVIIKRYKET
                     RRSHPLAEECDSLEGAIALERRMLAPLRERAEFVVDTSDLSTAKLRGELLRLFGRGSQ
                     EGAMTVSVTSFGFKHGLPREADLVFDVRFLPNPYYVQELRPRTGLDDGVRDYVFSGGA
                     AGEFLEKLQDLVGFLLPKYVEEGKTALVVAVGCTGGHHRSVAIAHALAAYIRGRGYPV
                     TESHRDLGRT"
     gene            complement(1294..1494)
                     /locus_tag="ACZPAQ_06510"
     CDS             complement(1294..1494)
                     /locus_tag="ACZPAQ_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903675.1"
                     /translation="MDGYTREELDEALTAVQSIIHKCEAARKKFPRGTAHHTLLERRL
                     RAMYLSRALICRELSGADGRER"
     gene            complement(1496..2428)
                     /gene="murB"
                     /locus_tag="ACZPAQ_06515"
     CDS             complement(1496..2428)
                     /gene="murB"
                     /locus_tag="ACZPAQ_06515"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR0903676.1"
                     /translation="MDRFELLRTELTRRCPGLELREEEPMARHTSFRIGGPARLMALP
                     RTEEEARETLQAAWALEIPPFFLGNGSNLLVADRGYEGFVVKPAGALEEIRALPPERP
                     GEKGRLEAGGAALLSRLSKAALDQGLTGLEFAQGIPGSVGGGVTMNAGAYGGETAQVL
                     LQVTALERDGTQRLLSGADCGLSYRHSRFSEGDLLICRAVFALPEGNREEIAGRMADF
                     AQRRREKQPLEYPSAGSTFKRPQGHFAAALIDQCGLKGRAVGGAQVSEKHAGFLINRG
                     GATCADVLELADQVRQEVLRQTGVELELEIRVLG"
BASE COUNT          422 a          953 c          798 g          457 t
ORIGIN      
        1 atggccagca cctggctcat ctgagccacc gcctcctcaa aggagccggt caccccgttg
       61 agggcggcca ggatcaggtt cccgaaggac tgtccagcca gggagccctc ctgaaagcgg
      121 taggtgagca gccgctccat gatcggctcc acgttggcca gggactccat gcagtggcgg
      181 atatccccgg ggggcggcat gccgatgtcc cggcgcagca cgccggagcc gcccccgtcg
      241 tcggccacag tgaccaccgc cgtcaggttt ttggtgtatt ttttcagtcc ccgcagcatg
      301 gtggacagcc ctgtcccccc gccgatggcg gtgatcttgg ggccgcgctc ccactggtgc
      361 agatttggaa tggtgtccgg catagcggga cctcccttca agtttgctct gtccgctcct
      421 tacgtcctgc ccaggtcccg gtggctctcc gtcaccgggt aacccctgcc ccggatatag
      481 gccgccagcg catgggcaat ggccaccgag cggtggtgtc cgccggtgca gcccaccgcc
      541 accaccagcg ccgtcttccc ctcctccaca tatttgggga ggagaaagcc caccaggtcc
      601 tgaagctttt ccaggaactc cccggccgct ccgccggaaa acacgtagtc ccttaccccg
      661 tcgtccaggc cggtgcgggg gcgcagctcc tgaacatagt aggggttggg caggaagcgc
      721 acgtcaaaga ccagatccgc ctctcggggc agtccgtgct tgaagccgaa ggaggtgacg
      781 ctcaccgtca tagccccctc ctggctcccc cgtccgaaga gtcggagcag ctcgccccgc
      841 agcttggcgg tggacaggtc ggaggtgtcc accacgaact ccgcccgctc ccgcaggggg
      901 gccagcatcc gccgctccag ggcgatggct ccctccaggg agtcgcattc ctccgccagg
      961 gggtggctgc ggcgggtctc cttataccgc ttgatgatca cgtcgtccga ggcgtccata
     1021 tacaggatgc ggtaggggca cttcatcccc ttcagcgcct ccagggactg gaacagggcg
     1081 ctgaaattgg tgccggagcg cacgtctgtg accagtacca cccggtcgta ctcccccgtc
     1141 ccggccatac ccagctcagc aaatttgggg atgagggggg cgggcagatt gtccacacag
     1201 aaaaagccca tgtcctccat gaaggaggcc gccttgctct tccccgcccc ggacaggccg
     1261 gaaatgatga taaattccat ggctgtacac ctcttatctc tctctgccgt ccgccccgga
     1321 cagctcccgg cagatcagcg cccgggacag gtacatggcc ctcagccgcc gctccagcag
     1381 ggtgtggtgg gcggtgcccc gcggaaattt cttccgggcc gcctcacact tatggatgat
     1441 ggactggacg gccgtcagcg cctcgtccag ctcctctctc gtatatccgt ccatcttacc
     1501 ccagcacccg gatctccagc tccagctcca cgccggtctg gcgcagcacc tcctgccgca
     1561 cctggtccgc cagctccagg acatcggcgc aggtggcgcc cccccggttg atgaggaagc
     1621 ccgcgtgctt ctccgacacc tgggcgcccc ccaccgccct gcccttcagg ccgcactggt
     1681 cgatgagggc ggcggcgaag tgcccctggg ggcgcttgaa ggtggagccg gcgctgggat
     1741 attccagagg ctgcttctct ctccgccgct gggcaaagtc tgccatccgc ccggcgatct
     1801 cctcccggtt tccctccggc agggcgaata ccgcccggca gatcagcagg tccccctctg
     1861 aaaaccggct gtgccggtag ctcaggccgc agtcggcccc ggagagcagc cgctgggtcc
     1921 cgtcccgttc cagcgcggtg acctggagga gcacctgggc ggtctccccg ccgtaggccc
     1981 ctgcgttcat cgtcaccccg ccgcccacgc tccccgggat gccctgggcg aactccaggc
     2041 cggtgaggcc ctgatccagt gcggccttgg agagccggga cagcagcgcg gcccctcccg
     2101 cctccagccg ccccttctcc cccggccgtt ccggggggag ggcccgtatt tcctccagcg
     2161 ccccggccgg cttgaccaca aagccctcat atccccggtc cgccaccagc agattggagc
     2221 cgttccccag gaaaaatggc gggatctcca gcgcccaggc cgcctgaagg gtctcccggg
     2281 cctcctcctc cgtcctgggc agggccatca gtcgggccgg gccgccgatg cggaaggagg
     2341 tgtgtcgggc cataggctcc tcttcccgca gctccagccc gggacagcgt cgggtcagct
     2401 ctgtgcgcag cagttcaaaa cgatccatag ggtcctcccg tccaatcaga atgaggtcgc
     2461 gcggagactt attttattat tatagcaaat ctctccggaa aatagaatac cccaaaacat
     2521 ctgttctttt ggtcagaagc gccagcggga gatccgcagg gctgaacggg gaccctgccc
     2581 cgcgtctgca cggggtgtgc cgcagcttcc acgcccccat gcatggcccg
//
LOCUS       DBMDSJ010000248         2617 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9401_length_2617_cov_1.733307, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000248 DBMDSJ010000000
VERSION     DBMDSJ010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9401_length_2617_cov_1.733307"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="ACZPAQ_06520"
     CDS             complement(<1..291)
                     /locus_tag="ACZPAQ_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR0903677.1"
                     /translation="MLDQEQELRFSKARRGAIAREFAHLNPEQQRAVLATEGPLLLLA
                     GAGSGKTTVLIHRIANLMKYGRGSDSPEVPERVTEDDLRFLEEYAASGAGDRA"
     gene            complement(432..587)
                     /locus_tag="ACZPAQ_06525"
     CDS             complement(432..587)
                     /locus_tag="ACZPAQ_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903678.1"
                     /translation="MAQLQLKGIDIGQSSLSALEGQRREASDREVLALSQIFQVPMER
                     LFQPHEE"
     gene            779..1132
                     /locus_tag="ACZPAQ_06530"
     CDS             779..1132
                     /locus_tag="ACZPAQ_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903679.1"
                     /translation="MSDSQTNFTLTDPAIEPIDLNRMDEFLFSAMLRASIHLGGSHTP
                     LQLSRMVRKSLLKLSLEKDLTFDLAYAKEFTPVSKLSPLALQIYEMLIAYTVQIVRNG
                     RCPASIPTPIQEETP"
     gene            complement(1350..>2617)
                     /gene="murC"
                     /locus_tag="ACZPAQ_06535"
     CDS             complement(1350..>2617)
                     /gene="murC"
                     /locus_tag="ACZPAQ_06535"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR0903680.1"
                     /translation="AHASGAVEHLRALGVPVTIGHLPESVEGADCVIRTAAVHDDNPE
                     IAAARRLGIPVFERAQAWGAIMRHYHNALCVAGTHGKTTTTSMCTHIFLAAGLDPTVM
                     IGGTLPAIGSGYRVGKGETIILESCEYCNSFLSFFPTVAVILNVDADHLDFFKDLEDV
                     EHSFRRFAELVPADTGVVVADRDDQNTMETLAGLERPLITFGLEEGDVHAAGLEWVNG
                     FPSFDIVFRGEKVTRVELQVPGEHNVRNALAAAAAALALRVPVRGIREGLGAFRGAGR
                     RFEHKGTYRGAQVYDDYAHHPEELRALLTMAQSLGYGRVICAFQPHTYSRTHELFSDF
                     VEVLKLPDQVLLAEIFAAREQNELGISSRDLAEQIPGAEFCPGVEQVAERLAQLAQPG
                     DLILTVGAGDIFRAGELLLAGQEKGPAGN"
BASE COUNT          461 a          896 c          753 g          507 t
ORIGIN      
        1 tgccctgtct cccgcgccgg aggcagcata ctcctccagg aagcgcaggt catcctccgt
       61 cacccgctcc ggcacctccg ggctgtcgga gccccggccg tatttcatca ggttggcgat
      121 gcggtggatg agcacggtgg tctttccact gccagccccg gccagcagca gcagcggccc
      181 ctcggtggcc agcaccgccc tctgctgctc cgggttcaga tgggcgaact cccgggcgat
      241 ggccccccgg cgggccttgg aaaatctcag ttcctgttct tgatccagca tatctctctg
      301 tcctcggttc ttcagtttca cagttaactc ctattgtacc gtaatccgtg gggcaggtca
      361 accgaaaacc cggccttccc tgggccgaag gggcggcatc cggtgggatg ccgccccctc
      421 tgaaatggga ctcactcctc atgcggctgg aacagccgct ccatgggcac ctgaaagatt
      481 tgggagagag ccagcacctc ccggtcactg gcctcccggc gctgaccctc cagggcggag
      541 aggctggact gcccgatgtc gatccccttc agttggagct gcgccaacag tattttctgt
      601 tttatgttct tttccttgcg aagcgaaacc acatttcttc caatacaatt cttatctcca
      661 tagtccctcc tgactctcac gggggatacc tccattttta tctggtaaac agattatatg
      721 gagaattccc gttgttatta tcggaaaaac agattataat ggtgacgagg tgattttaat
      781 gtcagatagt caaaccaact ttaccttgac tgatcccgcc atcgagccga tcgatctgaa
      841 ccggatggac gagttcctct tttcagctat gctgcgggcc tccatccacc tcggcggctc
      901 tcacacccct cttcagctgt ccaggatggt cagaaagtcg cttttgaagc tctctcttga
      961 gaaggatctg acctttgacc tggcttatgc caaagaattc acccccgtct ccaagctatc
     1021 ccccctggcg ctccagatct acgaaatgct tatcgcctac accgtccaga ttgtccggaa
     1081 cggccgctgc cccgcttcca tcccaacgcc gatacaggaa gagaccccct gaaccgcgca
     1141 gtttgaaata gacagcgatc cccacagggg ccccgccgct cctgtgggga tcattctatc
     1201 ctccgcctct tccagcagag atcgctccca aaagccttct cccggggaag gtgatacggc
     1261 gcatgccgcg acggatgtgg gggcggcttt ccgccgggcc gcatcacagc agcaggggcg
     1321 cacatggtgc gcccctgcaa acttcttttt cagtttccgg ccgggccctt ctcttgtccg
     1381 gccagcagca gctctccggc tcggaagata tctcccgctc ccacggtcaa aatcaggtcc
     1441 cccggctggg ccagctgggc cagccgttcc gccacctgct ccacaccggg gcagaactcc
     1501 gcgccgggga tctgttctgc caggtcccgg gaggagatgc ccagctcgtt ctgctcccgc
     1561 gccgcgaaga tctccgccag gagcacctgg tccggcagct tcagcacctc cacaaaatcg
     1621 gaaaacagct cgtgggtcct ggaataggta tggggctgaa aagcgcagat cacccgccca
     1681 tatcccaggc tctgggccat ggtcagcagg gcccgcagct cctcagggtg gtgggcgtag
     1741 tcgtcataga cctgcgcccc ccggtatgtc cccttgtgct caaagcggcg gcccgctccc
     1801 cggaaggcgc ccagcccctc ccggatgccc cgcaccggca cccgcagggc cagcgcggcg
     1861 gcggcggcgg ccagggcgtt gcgcacgttg tgctcccccg gcacctggag ctccacccgg
     1921 gtcaccttct ccccccggaa gacgatgtca aagctgggga agccgttcac ccactccagc
     1981 ccggcggcgt ggacgtcccc ctcctccagg ccgaaggtga tcagcggacg ctccagcccg
     2041 gccagggtct ccatggtgtt ctggtcgtcc cggtcggcca ccactacacc ggtgtctgcc
     2101 ggcaccagct cagcgaagcg gcggaaggag tgctccacgt cctccaggtc cttgaagaaa
     2161 tccaggtggt ccgcatccac attcaggatc accgccacgg tggggaagaa ggagaggaag
     2221 gagttgcagt actcacagga ctccaggatg atggtctccc ccttgcccac ccggtagccg
     2281 gagccgatgg cgggcagggt gccgccgatc atcacagtgg ggtccagccc tgcggccaga
     2341 aagatgtggg tgcacataga ggtggtggtg gtctttccgt gggtccccgc cacacacagg
     2401 gcgttgtggt agtgccgcat gatggctccc cacgcctggg cccgctcgaa gacggggatg
     2461 cccagccggc gggcggcggc gatctccggg ttgtcatcat ggacggcggc ggtgcggatg
     2521 acgcagtccg ccccctccac gctctccggc aggtggccga tggtcaccgg gacccccagc
     2581 gcccgaaggt gctccaccgc cccactcgcg tgggcgt
//
LOCUS       DBMDSJ010000249         2605 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9448_length_2605_cov_1.398562, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000249 DBMDSJ010000000
VERSION     DBMDSJ010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9448_length_2605_cov_1.398562"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /gene="hisS"
                     /locus_tag="ACZPAQ_06540"
     CDS             <1..1079
                     /gene="hisS"
                     /locus_tag="ACZPAQ_06540"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR0903681.1"
                     /translation="QLTFPFRRFQISKVYRGERAQRGRFREFYQADIDVIGDGALDIS
                     NEAEIPSIIYRAFTALGLKRFQIRVNNRKILNGFYAMLGLSQQSGEIMRTVDKLDKIG
                     PDKVAELLVQSGLSPEQAREILSFTSITGTNEEVLTALEGYRGRNEVFDLGLDELNAV
                     TKYLAAFGVPETHFAVDLTIARGLDYYTGTVYETTMLDHPEIGSICSGGRYDNLAEYY
                     TNKQLPGVGISIGLTRLFFVLEDQGYLNDALNTAPADALILPMTEDLAPAISLATQLR
                     QAGIRTQIQAERKKFKQKLSYADKLRIPYAVILGEDEIREGLAAVKDLATGEQVKLAP
                     GQAAAHILAGLDRLNQGTPILDRV"
     gene            1489..1689
                     /locus_tag="ACZPAQ_06545"
     CDS             1489..1689
                     /locus_tag="ACZPAQ_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903682.1"
                     /translation="MKIDYFQEGCSSTGDCQTTLLWDGTTLWVNWLGGHYLGYRPTDP
                     AGFEAAIQRLALEYGGTAIVTS"
     gene            1881..2360
                     /locus_tag="ACZPAQ_06550"
     CDS             1881..2360
                     /locus_tag="ACZPAQ_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903683.1"
                     /translation="MKKKLSFRLVVLVLLAVGVLHYWAAPTFDHAFQCNGIGPPAKPG
                     GSFQVLREGTYPGLSDAPVTFDEGRTYRELLAALRGQRYLRLPALLGAPDGGTAFYTA
                     SVCTPECMAYWDGTFLWLASNSSHWRRFLPLPPGRLGEKLEPIFMDEPEENIYYPSS"
     gene            2412..>2605
                     /locus_tag="ACZPAQ_06555"
     CDS             2412..>2605
                     /locus_tag="ACZPAQ_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903684.1"
                     /translation="MKKRALLSLALILALLAALYLWYTRPRSFLAITGTSENAVCSAA
                     LLLLEPWVDDGQAGQDTWEL"
BASE COUNT          492 a          850 c          733 g          530 t
ORIGIN      
        1 gccagctcac cttccccttc cgtcgcttcc agatcagcaa ggtctaccgg ggagagcggg
       61 cccagcgggg ccgcttccgg gagttctatc aggcggacat cgacgtcatc ggagacggcg
      121 ccctggacat cagcaacgag gcggagatcc cctccatcat ctatcgggct ttcaccgccc
      181 tggggctgaa gcgcttccag atccgggtga acaaccggaa gatcctcaac ggcttttacg
      241 ccatgctggg tctttcccag cagtccggcg agatcatgcg cacggtggac aagctggaca
      301 agatcggccc ggacaaggtg gccgagctgc tggtgcagtc cggcctctct ccggagcagg
      361 cccgggagat cctcagcttt acctccatca ctggcaccaa cgaggaggtc ctgaccgccc
      421 tggagggcta ccggggccgg aacgaggtct ttgacctggg cctcgatgag ctgaacgcgg
      481 tgacaaaata tctggctgcc ttcggcgtgc ctgagactca cttcgccgtg gacctgacca
      541 tcgcccgggg actggactac tacaccggca ccgtctatga gaccaccatg ctggaccatc
      601 cggagatcgg ctccatctgt tccggcggcc gttatgacaa cttagcggaa tattacacga
      661 ataaacaact ccctggagtg ggcatttcca tcggtttgac ccgcctgttc ttcgttctgg
      721 aggaccaggg ctatctcaac gacgccctga acaccgcccc cgccgacgcc cttatcctcc
      781 ccatgaccga ggacctggcc cctgccatct ccctggccac ccagctgcgc caggcaggca
      841 tccgcaccca gatccaggcg gagcggaaga agttcaagca gaagctctcc tatgcggaca
      901 agctccgcat cccctacgcc gtcatcctgg gagaggacga gatccgggag ggcctcgccg
      961 ccgtcaagga cctggccacc ggggagcagg tgaagcttgc tcccggccag gctgccgccc
     1021 acatcctggc cgggctggac aggctgaacc aggggacgcc gatcctggac agggtgtaag
     1081 tccgatttcc catccacgct ttggctgtgc aggagccgat atccccaccg ggccgtccca
     1141 cgccgtatag atggaattcc cgggccagct gtctctgata tagaaagaga aaagctcatg
     1201 aagaaaaatg ccaaacgctt tttactgctt ttaatcgttt gtcttttggt gtggacggta
     1261 tttccccggc cctttgcctg gatcattcct cagcggatgg taaatgtctc aggagaaaga
     1321 ccggaaatct gcgccgagac agaattcacc gcttctctca gctttggcat tgaccaagaa
     1381 gtcttggatg ccttttatga tctccatagt gagatggaag gcgtccggtt cctggccgat
     1441 cccctctccc tcctgccttt cctccaccag ccccgggagc cggtcaaggt gaaaatcgat
     1501 tacttccagg agggctgttc ttccactggc gactgccaga ccaccctgct gtgggatggt
     1561 acgaccctgt gggtgaactg gctgggcggc cactacctgg gttaccggcc cactgatccc
     1621 gccggatttg aggcagccat ccaacggctg gccctggaat acggcggaac tgcaatcgtc
     1681 acctcgtagg gacggcacat ctggtccgcc tgtccttccc agaataataa gcctctaaaa
     1741 atttcacaac acgcaaagcg gctttccctt tgaccgcgcg gctactatga ccatgtcatc
     1801 cgcaaccaac aggaccttga cgagaccgcc gggtacatcc ttggcaatcc cgcccggcgg
     1861 ctggagcggg agggatcctt gtgaagaaaa aattgagctt tcgtctggtc gttttggtgc
     1921 tcctggccgt cggggtgctg cactattggg cggcccccac cttcgaccat gccttccagt
     1981 gcaacggcat aggtccgccc gcaaaacctg ggggttcttt tcaggtgctg cgcgagggca
     2041 cctatcccgg cctcagtgac gctcctgtca cctttgatga ggggcggact taccgtgagc
     2101 tgctggccgc cctccggggg cagaggtatc tccgcctccc cgccctgctg ggggcccccg
     2161 acggcgggac cgccttctat accgcatccg tctgtacccc cgagtgcatg gcctactggg
     2221 acggcacctt tctctggctg gcctcgaact cctctcattg gcgccgcttt ctcccccttc
     2281 cccccggccg cctgggggag aagctggagc ccatttttat ggacgagccg gaggaaaaca
     2341 tttactaccc ttcctcttag gcgcaggtcc tttgacccgc cctgccggta aaaagcaagg
     2401 aggtgcgccc catgaaaaaa agagccctgc tctccctggc cttgatttta gcccttctgg
     2461 ccgctctata cctgtggtac acccggcccc ggagcttttt ggcgatcact gggacctcag
     2521 agaacgccgt ctgctccgcc gccctgcttc tgttagagcc ctgggtggat gacggccaag
     2581 caggacagga tacctgggag cttca
//
LOCUS       DBMDSJ010000250         2603 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9460_length_2603_cov_1.554756, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000250 DBMDSJ010000000
VERSION     DBMDSJ010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9460_length_2603_cov_1.554756"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="ACZPAQ_06560"
     CDS             <1..422
                     /locus_tag="ACZPAQ_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903685.1"
                     /translation="MALALLRRQGEDPLRAAVALRGSRAERDLVRAAEELCPRVRDLC
                     LSVQTGGGELERYLHRQYGVALRPDWDGVQAAIRFDPRAEEAGQAVLSLFGPEADLAG
                     LVVSVPGLERQKEGQWLPLLEILWETGRLERTDLEFT"
     gene            545..1021
                     /gene="greA"
                     /locus_tag="ACZPAQ_06565"
     CDS             545..1021
                     /gene="greA"
                     /locus_tag="ACZPAQ_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR0903686.1"
                     /translation="MAKEYKLSPQRLKELQDEMNYLKTVREKEVAELIKEARSFGDLS
                     ENSEYDEAKNEQGKLYSRMAEIEEILSNYVVIEEEPSDGSSVRLGSTVTVLDEEFDET
                     MVYKIVGSQEADPMNGAISEDSPFGKALLGKSEGDQVVVDAPAGRIEYKLLKVEQR"
     gene            1058..2548
                     /gene="lysS"
                     /locus_tag="ACZPAQ_06570"
                     /pseudo
     CDS             1058..2548
                     /gene="lysS"
                     /locus_tag="ACZPAQ_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
BASE COUNT          535 a          771 c          852 g          445 t
ORIGIN      
        1 ctatggctct ggcgctgctg cgccgccaag gggaggaccc gctgcgggcg gcggtggccc
       61 tgcgggggag ccgggcggag cgggatctgg tccgggcggc ggaggagctg tgccccaggg
      121 tgcgggattt gtgcctctcc gtacagacgg gcggagggga gctggaacgc tacctccacc
      181 gacagtacgg tgtggccctg cggccggact gggacggcgt ccaggcggcc atccgcttcg
      241 atccccgggc ggaggaggcg gggcaggcgg tcctctccct gtttgggccg gaagcggatc
      301 tggcggggct ggtggtgtcg gtccccggct tagagcggca gaaggagggc cagtggctgc
      361 ccctgctgga gatcctgtgg gagacagggc gtttggagcg cactgaccta gaatttactt
      421 gacagacgga gccgaaaaca ctataatagc atatactgca tccatatacc cattttcata
      481 ttctggaacg atcatccgcc ccggggccgc cccggagagc ggtctgaaag gtgtgcaacg
      541 agtcatggca aaggaatata aactcagccc ccagcgcctg aaagagcttc aggacgagat
      601 gaattatctg aagacagttc gggagaagga agtggccgag ctgatcaagg aggcccgctc
      661 tttcggagac ctgtctgaga acagcgagta cgatgaggcc aagaacgagc agggcaagct
      721 ttactcccgc atggctgaga tcgaggagat cctgtccaac tatgtggtca tcgaggagga
      781 gccctctgac ggcagctctg tccgcctggg ctccacggtc accgtgctgg acgaggaatt
      841 cgacgagact atggtgtaca agatcgtggg ctctcaggag gccgacccca tgaacggggc
      901 catctcggag gactccccct tcggcaaggc cctgctgggc aagagcgagg gagaccaagt
      961 ggtggtggac gcccccgccg gacgcatcga gtacaagctg ctgaaggtgg agcagcgctg
     1021 agcgccccgc catccacaga gataggagag agacaacatg gctgaagaga agaaccagga
     1081 gcagaacctg tcccagctgc tccagatccg ccgggacaag ctgaaggagc tccagggggg
     1141 ggggcgtgac cccttccaga tcaccaaata tgaggtcacc cacgacagcg ccgccatcaa
     1201 ggcggatttt gacgccatgg agggcagcca agtctcgctg gccgggcggc tcatgtccaa
     1261 gcggggcatg ggcaaggtgt ccttctgcga cctgcaggac aagtcgggcc gcatccagct
     1321 ctatgcccgc cgggacgagc tggatgggga gtagtacgac cgcttcaaga agctggatat
     1381 cggcgacatc gtgggagtga agggcatcgt gttccgcacc cagcgggggg agatgtccat
     1441 ccgggtggag gcggtgaccc tgctgtccaa gtcccttctg cccctgccgg agaaatttca
     1501 cggcctgacc aacaccgagc tgcgctaccg ccagcgatat gtggacctga tcgtcaaccc
     1561 ggaggtgaag cgcaacttca tcatccgctc ccagttcatc aagcatgtgc gggacttcct
     1621 ggacggccgg ggctatatgg aggtggagac ccccgtcctg aacaccatct ccggcggcgc
     1681 cactgcccgt cccttcatca cccaccacaa caccctggac atcgacatgt tcatgcgcat
     1741 cgccaccgag ctgcccctga agcgtctcat tgtgggcggt ctggaccggg tgtatgagat
     1801 cggccgcatt ttccgcaatg agggcatgga cccgaagcac aatccggagt tcaccaccgt
     1861 ggagctctac caggcctacg ccgatttcaa cgacatgatg gatctgttcg aggacctgct
     1921 ctcctccgcc gcccagaaga tcctgggcac ctatcaggtg gagtggcagg gggagcagat
     1981 cgacctgact cccggatggc cccgcatgcc tatgcatgag gcggtgaagc agtactgcgg
     2041 catcgacttc atggccatca acagtgacga ggaggcggtg gccgccgcca agtccatcgg
     2101 cgtggagctg cccgagaccg cggacaagac ctggggcaac gccctgtatg agtgctttga
     2161 tcagaaggtg gaggagaagc tgatccagcc caccttcatc accatgcatc cggtggatgt
     2221 gtcccctctg gccaagcggt cccccaagga cccccggctc accgagcggt tcgagctgtt
     2281 catctgccac agtgagatgg gcaacgcctt ctccgagctc aacgacccca tcgaccagcg
     2341 gggccgcttc cagaagcagg tggagctgcg ggataaggga gatgacgaag ccggcatgat
     2401 ggacgaggac ttccttaccg ccctggagta cggtatgcct cccacgggcg gtctgggcat
     2461 cggcatcgac cgctgtgtca tgctgctcac caacagcgat tccatccggg aggtcatttt
     2521 gttcccgacg atgaagcccc tcgattgaga aacccttgca gcgcaagggg ttgcgggctt
     2581 tttcaacaca aggaaggccg gtt
//
LOCUS       DBMDSJ010000251         2600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9478_length_2600_cov_2.106042, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000251 DBMDSJ010000000
VERSION     DBMDSJ010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9478_length_2600_cov_2.106042"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(30..284)
                     /locus_tag="ACZPAQ_06575"
     CDS             complement(30..284)
                     /locus_tag="ACZPAQ_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903687.1"
                     /translation="MGLHVLPGYGQPLLVLLPAFLPARLSRARPQSLPAHLPRAWACS
                     LPAYLPWAWPCSLPAYLPRAHPARRDRPHRPHRPTGYPWT"
     gene            508..2010
                     /gene="glpK"
                     /locus_tag="ACZPAQ_06580"
     CDS             508..2010
                     /gene="glpK"
                     /locus_tag="ACZPAQ_06580"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR0903688.1"
                     /translation="MSDRPYVLALDQGTTSSRCILFDRQQNIVGLAQREFGQHYPKPG
                     WVEHDPMEIYSSQYSVLTEVLAQSGVDPAQVAAIGITNQRETTIVWDRETGRPVYNAI
                     VWQCRRTAALCEELKADDELTSYIREHTGLLPDAYFSATKLKWVLDHVEGARERARRG
                     ELLFGTVDSWLIWKLTGGRVHVTDYTNASRTMLFDIQRLCWDEHICRTLDIPMEMLPQ
                     VRDSSAVYGTVNLQGVEVPIAGIAGDQQAALFGQACFSPGEAKNTYGTGCFLLMNTGR
                     ELCRSRNGLLTTIAVGLNGSVQYALEGSVFVGGAVIQWVRDELRFFSESRDAEYYAQK
                     VPDTGGVYLVPAFTGLGAPYWDMYARGCLVGLTRGTTREHIIRAAQESIAYQSWDLVR
                     AMEQDTGLPLAELRVDGGASRDRFLMQFQADILDKPVRRPMIRETTALGAAYLAGLAV
                     GVWDSLDEIRAQWTLDRLYQPQMEEPVRRSLLAGWHKAVGRSLNWAEPET"
     gene            complement(2295..>2600)
                     /locus_tag="ACZPAQ_06585"
     CDS             complement(2295..>2600)
                     /locus_tag="ACZPAQ_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903689.1"
                     /translation="ALVQPEDGGPVSLTKDGAVTGGLRLLGGGGDRALEHDILWSSYR
                     YGTGAYSRSGVVCQEGERLVRYFVDCGDSGICELWLDRRRVLPVEEEQLLDRFTGMA"
BASE COUNT          474 a          818 c          854 g          454 t
ORIGIN      
        1 cctgagggcc ggtagggcca gtggcaccgt taggtccagg gataccctgt gggccggtgg
       61 ggccggtggg gccggtcccg acgggcggga tgggcccggg gcagataggc gggcagggag
      121 cagggccagg cccagggcag ataggcgggc agggagcagg cccaggcccg gggcagatgg
      181 gcgggcaggg actggggccg ggcccgggac agacgggctg gcaggaaggc gggcaggagc
      241 acgagcagcg gctgcccgta ccctgggagc acatggagtc ccatcgtgtc agacttctgt
      301 ctgaaggaag ataacggttc atttgggtct ccttttcttc attcaagatt gcgtggcttt
      361 gccacagccc attctatgcg actgccgttc ttccggtgcg cctccgcgcc cggccgtcct
      421 ttcccgcagc cgccccttta caatccagaa catttctgac ataatggagg caaaccacca
      481 ccattcacca ggaaaggccg tgttgttatg tctgaccgcc cctatgttct cgccctggat
      541 caaggcacca ccagcagccg atgcatcctg ttcgaccggc agcagaatat tgtcggcctg
      601 gcccagcggg aatttggaca gcactaccca aagcccggct gggtggagca tgatcccatg
      661 gagatctatt ccagccaata cagtgtcctc accgaggtgc tggcccagag cggggtggac
      721 cccgcccagg tggcggctat cgggatcacc aaccaacggg agaccaccat cgtttgggac
      781 cgggagacag gcaggccggt gtacaacgcc atcgtctggc agtgccgccg caccgcggcc
      841 ctgtgcgagg agctgaaggc ggatgacgag ctcacctcct acatccggga acacaccggc
      901 ctgctgccgg atgcctattt ttccgccacc aaactgaagt gggtcctgga ccacgtggag
      961 ggggcaagag agcgggcccg tcggggggag ctgctctttg gaacggtgga ctcctggctc
     1021 atctggaagc tcaccggcgg ccgggtccac gtcaccgact ataccaacgc cagccgtacc
     1081 atgctcttcg acatccagcg cctgtgctgg gatgagcaca tctgccggac gctggacatc
     1141 cccatggaga tgctgcctca ggtgagggac tccagcgcgg tgtatggcac tgtgaacctc
     1201 cagggagtgg aggtccctat cgccggcatc gccggtgacc agcaggcggc cctgttcggg
     1261 caggcctgct tctctccagg ggaggccaaa aacacctatg ggactggctg cttcctcctg
     1321 atgaacactg ggcgggagct gtgccggagc aggaacggtc tgctcaccac catcgctgtg
     1381 ggcctcaacg gttcggtgca gtatgcactg gagggctctg tcttcgtggg cggagcggtg
     1441 atccagtggg tacgggacga gctgcggttt ttcagcgagt cccgggatgc ggagtactac
     1501 gcccagaagg tgccggatac cggcggggtc tatctggtcc cggccttcac cggcctggga
     1561 gccccctatt gggatatgta tgccaggggc tgcctggtgg ggctcacccg gggcacgacc
     1621 cgggaacaca ttatccgagc ggcccaggag tccatcgcct accagagctg ggatctggtc
     1681 cgggccatgg agcaggacac cggcctgccc ctggccgaac tccgggtgga cggcggggcc
     1741 agccgggacc ggtttttgat gcagttccag gcggatatcc tggacaagcc ggtccgccgc
     1801 cccatgatcc gggagaccac cgctctgggg gctgcctatc tggcgggcct tgcggtgggg
     1861 gtgtgggaca gtctggatga gattcgcgcc cagtggaccc tggacagact gtatcagccc
     1921 cagatggagg agcccgtccg ccggtctctg ctggctggat ggcacaaagc cgtgggccgc
     1981 agcctgaatt gggcagagcc ggagacatga taaaaggtgg cgtccatccg cacactgcgg
     2041 atggacgcct cagcttgtca aaaagccctc ctgcaaggag ttccggcgcc cgcaggcacc
     2101 ggaatcaaat tggagcacgt catttccggg ggcgtgtacc tcggaaatga cacctctcac
     2161 gcaagatagg acgacttttc cgagaggaat caaggcctat cttgcgtgct tactttctcg
     2221 aaaaagcggc tctgccgctt tttcgacagt ctgtggcgtc catccgcaca ctgcggatgg
     2281 acgcctttcg actcttatgc catgcctgtg aaccggtcca ggagctgctc ctcctccaca
     2341 gggagcaccc gcctccggtc cagccacagc tcacagatcc cagagtcgcc gcagtccaca
     2401 aagtagcgga ccagccgctc cccttcctga cacaccactc cggagcggct gtatgctccg
     2461 gtcccgtagc ggtaggagga ccacagaatg tcgtgctcca gcgcccgatc cccgccgccc
     2521 cctagaagcc gcagcccgcc agtgactgcg ccgtccttgg tgagcgagac agggccgccg
     2581 tcctccggct ggaccaaggc
//
LOCUS       DBMDSJ010000252         2596 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9498_length_2596_cov_1.699399, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000252 DBMDSJ010000000
VERSION     DBMDSJ010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9498_length_2596_cov_1.699399"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..1228)
                     /locus_tag="ACZPAQ_06590"
     CDS             complement(68..1228)
                     /locus_tag="ACZPAQ_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR0903690.1"
                     /translation="MLIIHGTVHTMDGPAIDNGFVAIREGKIWKVGPMEECPADWKGE
                     TLDARGGHILPGFVDAHCHLGMFGDAMGFEGDDGNEATDPCTPHLRAIDGVNPMDRCF
                     RDARLAGVTTVLTGPGSANPISGQFVAMKTAGRWLDDMVIKAPAAMKLALGENPKLTY
                     HERHEGPITRMATAAVIRENLAKAVEYRDKLERAAADPDEEKPEFDAKLEALLPVVRR
                     ELPVHIHAHRADDIATGIRICKEFGLEYVIVHGTEGHLIANELAREGARVITGPCLGD
                     RSKPELANMSLETPVLLAMAGVKIAICTDHPETPVQYLPLCAAMAVKGGLPADAALAA
                     ITIWAAEIGGVAHRVGSLTPGKDGDVVVMSGHPLNWLSRVSAVVVDGRRVTE"
     gene            complement(1432..1875)
                     /locus_tag="ACZPAQ_06595"
     CDS             complement(1432..1875)
                     /locus_tag="ACZPAQ_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR0903691.1"
                     /translation="MEFGVGSIVDGKVTGITKFGAFVALPEGRSGLVHISEIAYSYVN
                     EVSDHLKEGQEVKVKVIGIDQNNRINLSIKKVTEPPPRPAGGGQGGRPRSGGGRPGGF
                     APRPAAPKEPTDFEDRLKQFMQSSDSKLSELRYIEKKGNSRRSRR"
     gene            complement(1931..2206)
                     /locus_tag="ACZPAQ_06600"
     CDS             complement(1931..2206)
                     /locus_tag="ACZPAQ_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR0903692.1"
                     /translation="MRFKRAGLLTKVVVMVLLIYMATALLDLHGQIQTIQVEREELNQ
                     QVAAQQLENQQLANAIENSDDPDMLESVARDKGYVKSGETLYIDVAN"
     gene            complement(2445..2594)
                     /gene="yabQ"
                     /locus_tag="ACZPAQ_06605"
                     /pseudo
     CDS             complement(2445..2594)
                     /gene="yabQ"
                     /locus_tag="ACZPAQ_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021118.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
BASE COUNT          459 a          857 c          731 g          549 t
ORIGIN      
        1 acaggcgtca aaaaattcta gacttttttt gaatttttct caagagtttc ccttttctaa
       61 cagaacatca ttccgtcacc cgccggccgt ccaccaccac ggcggagacc cggctgagcc
      121 agttgagggg gtggccgctc atcaccacca cgtcaccgtc cttccccggc gtcagggagc
      181 ccacccggtg agccactccg ccgatctccg ccgcccagat ggtgatggcc gccagggccg
      241 catccgccgg caggccgccc ttcactgcca tggcggcaca caggggcagg tactggaccg
      301 gggtctccgg gtggtcggtg cagatagcga tcttcacccc cgccatcgcc agcagcaccg
      361 gggtctccag gctcatgtta gccagctccg gtttggaacg gtcccccagg caggggccgg
      421 tgatgacccg ggccccctcc cgggccagct cgttggcgat gaggtggcct tccgtaccgt
      481 ggacgatgac atactccagc ccgaactcct tgcagatgcg gatgccggtg gcgatatcgt
      541 ccgcccggtg ggcgtggata tggacaggca gttctcggcg gaccacaggg agcagggcct
      601 ccagcttggc gtcaaactcc ggcttctcct cgtctgggtc ggcagccgcc cgctccagct
      661 tgtcccggta ctccactgcc ttggccagat tctcccggat gacggcagcg gtggccatcc
      721 gggtgatggg tccctcatgc cgctcgtggt aggtgagctt ggggttctct cccagcgcca
      781 gcttcatggc cgccggggcc ttgatgacca tgtcgtccag ccagcggccg gcggtcttca
      841 tggctacgaa ctggccgctg atggggttgg cgctgcccgg accggtcagg accgtggtca
      901 ctcccgccag ccgcgcgtcc cggaagcagc ggtccatagg gttcactccg tcgatggccc
      961 gcaggtgggg ggtgcagggg tcagtggcct cgttgccgtc gtccccctca aagcccatgg
     1021 cgtccccgaa catccccagg tggcagtggg cgtccacgaa gccaggcagg atgtgccccc
     1081 cccgggcatc cagcgtctcc cccttccagt cggcgggaca ctcctccatc ggtcccacct
     1141 tccagatctt tccctcccgt atggctacaa acccgttgtc gatggccggt ccgtccatgg
     1201 tgtgcaccgt tccgtgaatg atcagcatat attgctcctt tccggcaatt ctttagttga
     1261 catatttgta attatatggt atatttgtca ggcagctcaa ccaaactggt tttctctgcg
     1321 aatctgttcc tgcatttgct ggacgccctt ggatggagat ccatccgggg gcgtttttgt
     1381 atgccgggat aaaaaaggac cgcgcaggca gctgcgcggt cccggtgcct ctcaccggcg
     1441 ggagcggcgg gagtttccct ttttttctat gtagcgcagc tcagacagct tactgtcaga
     1501 ggactgcata aactgcttca gccggtcctc aaagtcagtg ggctccttgg gcgcggcagg
     1561 ccggggcgcg aagccgccag gccgccctcc cccggaacgg ggccgtccgc cctgtccgcc
     1621 gccggccggg cggggagggg gctctgtcac cttcttgatg gacaaattga tccggttgtt
     1681 ctgatcgatg ccgatgactt ttactttgac ttcctgcccc tccttgaggt gatccgacac
     1741 ctcgttcaca taggaataag cgatctcaga gatgtggacc aagccggatc tcccttccgg
     1801 cagggccaca aaggcaccga attttgtgat tcccgtaacc ttcccatcca caatggaccc
     1861 aacaccaaac tccataagcg actgatatat cctccttgta aatttccccc tgaggccgtt
     1921 tcctgggtgg tcagttggcc acgtcgatgt agagggtctc ccccgacttc acatagccct
     1981 tgtcccgggc gaccgactcc agcatgtcag gatcgtcgct gttttcgatg gcgttggcca
     2041 gctgctggtt ttccagctgc tgggcggcca cctgctgatt cagctcctcc cgctccacct
     2101 ggatggtctg gatctgccca tggagatcca gcagggccgt ggccatgtag atcagcagga
     2161 ccatcaccac gaccttggtc aaaagaccgg ctcgcttgaa ccgcatgcgc actccctccc
     2221 gtctcccgcc gctcccgtct ccgggcggcg gcatccagtt cctcagttat ctgatttatt
     2281 ctaaaccatt cctgcgcaaa atgaaagcta ttttttgcaa attttttagt ttttttctgc
     2341 aattttcccg ccgccgccag ggggagggta aggatgcgcc acaccagggc cgccgcatct
     2401 gccaaaaaat agccgatttt cagcatccag gtactgatga gccagaaata gagcaccccg
     2461 cccccaaaga gaaagaagat cccgtacagc cggatctccc cccgccatgc ctgctgagac
     2521 cagacgaaca gcgccactgt cacaatgatc cagaaggaca gatccagcac cgcccccagt
     2581 aggggcaggg ccaccc
//
LOCUS       DBMDSJ010000253         2590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9528_length_2590_cov_2.161912, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000253 DBMDSJ010000000
VERSION     DBMDSJ010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9528_length_2590_cov_2.161912"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="ACZPAQ_06610"
     CDS             <1..571
                     /locus_tag="ACZPAQ_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HIR0903693.1"
                     /translation="PIEREIYGNKAAAAAGISAGAFAQEVERFRKNRAWQARKKQARR
                     ELTPAAQLQPRERELRYENLRSARAEEGVVRLLLLEPDLFPQVEGLGPEQFSAPVLAK
                     IYALLCQRHREGRSTQLAALAGALSPEEMSHLVSVMDQPEALAHSAQALRDYIEIIET
                     EALKRGGAGASDPLLAAREKFRERKAYGG"
     gene            576..1922
                     /gene="rpoD"
                     /locus_tag="ACZPAQ_06615"
     CDS             576..1922
                     /gene="rpoD"
                     /locus_tag="ACZPAQ_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR0903694.1"
                     /translation="MSEKKTTSKKVHTLTLEDQTKLAQANVKKMEQGKIEVMKVVEGV
                     QGAEKLAELIERGKKNGKLSSSELMDVLENLDLESDQMDKIYDTLENLGIDTVGDDYL
                     PELADDIMPPLEELNEIEELPEEEMVDPNTLVDSFGIDDPVRMYLKEIGKVNLLTPDE
                     EIQLAQDMGAGDAAKEQLEELERARKNGEESSISEEDEKELRRLVKRGEAAKQRLAEA
                     NLRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAIT
                     RAIADQARTIRIPVHMVETINKVIRVSRQLLQELGHDPSPEEIAEEMSMPVDKVREIL
                     KIAQEPVSLETPIGEEEDSHLGDFIPDEDASEPSEAASFTLLKEQLVDVLGTLTPREE
                     KVLKLRFGIEDGRTRTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLN"
     gene            complement(2426..>2590)
                     /locus_tag="ACZPAQ_06620"
     CDS             complement(2426..>2590)
                     /locus_tag="ACZPAQ_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013777.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR0903695.1"
                     /translation="EVPPRVEYTLTAMGESFLPVLNHMLLWSETHLCPPDYVNPYQDG
                     GCSPLPGDDA"
BASE COUNT          535 a          791 c          881 g          383 t
ORIGIN      
        1 tcccatcgag cgggagatct acggcaacaa ggccgcggcc gccgcgggca tttcggccgg
       61 ggcctttgcc caggaggtgg agcggttccg caagaaccgg gcatggcagg cccgcaagaa
      121 gcaggcccgc cgggagctga cgccggcggc ccagctccag ccccgggagc gggagctgcg
      181 gtatgagaac ctccgctccg cccgggcgga ggagggggtg gtgaggctgc tgctgctgga
      241 gcccgacctg ttcccccagg tggaggggct ggggccggag cagttttccg ccccggtgct
      301 ggccaagatc tacgccctgc tgtgccagcg ccaccgggag gggcgctcca cccagttggc
      361 cgccctcgcc ggggcgctct ccccggagga gatgagccac ctggtgagcg tgatggatca
      421 gccggaggcc ctggcgcaca gcgctcaggc ccttcgggat tacatagaga tcattgagac
      481 cgaggccctc aagcggggcg gggcgggcgc aagcgacccc ctgctggcgg cacgggagaa
      541 gttcagagaa agaaaagcgt atggaggata accgcatgag tgagaagaag accaccagca
      601 agaaggtaca caccctgacc ctggaggatc agaccaagct ggcccaggcc aatgtgaaga
      661 aaatggagca gggcaagatc gaggtcatga aggtcgtcga gggcgtccag ggggcggaaa
      721 agctggcgga gctgatcgag cggggcaaga aaaacggcaa gctctcatcc agtgagctca
      781 tggatgtgct ggagaacctg gatctggaga gcgaccagat ggacaagatc tacgataccc
      841 tggagaacct gggcatcgac accgtgggcg atgactatct gcccgagctg gccgatgata
      901 tcatgccccc cctggaggag ctcaacgaga ttgaggagct ccccgaggag gagatggtgg
      961 atcccaatac gctggtggac tccttcggca tcgacgaccc tgtgcggatg tacctcaagg
     1021 agatcggcaa ggtgaatctg ctcacccccg acgaggagat ccagctggcc caggacatgg
     1081 gcgccggcga cgcggccaag gagcagctgg aggagctgga gcgggcccgc aagaacggcg
     1141 aggagagctc catctctgag gaggacgaga aggagctgcg ccgcctggtg aagcggggcg
     1201 aggccgccaa gcagcgcctg gccgaggcca acctccgtct ggtggtgtcc atcgccaagc
     1261 ggtacgtggg ccgcggcatg ctcttcctgg atctgatcca ggagggcaac ctgggcctca
     1321 tcaaggcggt ggagaagttc gactacacca agggctacaa gttctccacc tatgccacct
     1381 ggtggatccg ccaggccatc acccgggcca tcgccgacca ggcccgcacc atccgcatcc
     1441 ccgtccacat ggtggagacc atcaacaagg tcatccgcgt ctcccgccag ctcctccagg
     1501 agctgggcca cgacccctcc cccgaggaga tcgccgagga gatgagtatg cccgtggaca
     1561 aggtgcggga gattctgaaa attgcccagg agccggtgag cctggagacc cccatcggcg
     1621 aggaggagga ctcccacctg ggcgacttca tccccgacga ggacgcctcc gagccgtcgg
     1681 aggccgcctc cttcaccctg ctcaaggagc agctggtgga cgtgctgggt accctgaccc
     1741 ccagggagga gaaggtgctc aagctgcgct tcggcattga ggacggccgc acccgcaccc
     1801 tggaagaggt gggcaaggag ttcaacgtca cccgtgagcg catccgccag atcgaggcca
     1861 aggctctgag aaaactgcgc catcccagcc gttccaagaa gctgaaggac ttcctgaact
     1921 gattcttttt tgatgccgtc aggcattggg cgcagtttcg gggtccccgc gggagcccag
     1981 cgcaagcggg tcccgtgggg agaggaggag ccgcggagcg agggagcctt cgccgtccgg
     2041 cggaggcgag gaatgcgcag cccgcgacga cgacgcgcca ccccagccgc tccaagaagc
     2101 ggaaggactt tctgaactga gacagtacag cgacgagacg aggcccggaa ccatatggtt
     2161 ccgggcctct gatctgtcga agatctcccg caaaggaaag ccgcgcagag ttcctgcggc
     2221 agcagccgca ggaatgaaac gaaaatccgt catttccggg gacatgtgcc tcggaaatga
     2281 cacttctcat ggcggaaggg gcgactattt cgctggaaat cgagtccctt ccgccatgct
     2341 gccttactcg aaaaagtggc gctgccactt tttcgacacg ctggcgaggc ccggaaccgt
     2401 atggttccgg gcctcgctgc gtctcctatg catcgtctcc ggggagcggg ctgcaccccc
     2461 cgtcctggta ggggttgacg taatccggag ggcagaggtg cgtttcgctc cacagcagca
     2521 tgtggttcag cacaggcaga aagctctcgc ccatggccgt gagggtgtac tccacccggg
     2581 gcggaacctc
//
LOCUS       DBMDSJ010000254         2583 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9562_length_2583_cov_1.718775, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000254 DBMDSJ010000000
VERSION     DBMDSJ010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9562_length_2583_cov_1.718775"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="argB"
                     /locus_tag="ACZPAQ_06625"
     CDS             <1..1046
                     /gene="argB"
                     /locus_tag="ACZPAQ_06625"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR0903696.1"
                     /translation="CDYSIEPNRGEAIRQAVLGCREPSVLLITGKGAETRQKRGTEYI
                     DTPSDVDYVQSFLQEYDVRHGLDGMEKVRSLLSILPILKRDEGRTVVVKYGGSALGAE
                     AATDTTLQDVAALRMVGVRVVLVHGGGKHITALLDKLQVPTRFENGYRYTDQAVLETA
                     EMALSAQVNKAIVSRLSQLEVSAVGLSGKDGGLLTAVEKDPALGRVGSITRVEPRILQ
                     TLLDGDFLPVVSPIAAGEDGGGFNCNADDAARAVAAALGADKLIFLTDTAGVLIDSHN
                     SKTAVPHMDVKRAEELIDTGLIAGGMVPKVRGCIQAIRAGVGEVSILDGRVEHALLLE
                     MLNQRVQGTTITG"
     gene            complement(1208..1408)
                     /locus_tag="ACZPAQ_06630"
     CDS             complement(1208..1408)
                     /locus_tag="ACZPAQ_06630"
                     /inference="COORDINATES: protein motif:HMM:NF039477.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HIR0903697.1"
                     /translation="MTFQEAGARQVYGIDCYRRDRKDAPPVAQYRDVSIDRGEMDRLA
                     DRCNRGQLDPLQLEDVILDAIL"
     gene            complement(1416..1790)
                     /locus_tag="ACZPAQ_06635"
     CDS             complement(1416..1790)
                     /locus_tag="ACZPAQ_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903698.1"
                     /translation="MERTRPAGDAEVERTDRSGPVCAASVADGCGPEDGLILQTGGEL
                     MALKWRFSRLLYTWRRENELTQEEAAERCGVSPRHYLDMEAGRVNPKLSTVIHIASES
                     NISLDVLKSEVRGTHAVSLPPE"
     gene            complement(1806..2243)
                     /locus_tag="ACZPAQ_06640"
     CDS             complement(1806..2243)
                     /locus_tag="ACZPAQ_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903699.1"
                     /translation="MKKKISRGCAVLLACTMLAGLLMTTAFARASDYLAIYSVWATAK
                     SDGVVRIHADVDAVRTSDEVGVSYITVQESRDGGDTWTTLETFTKEDLNMYEENTDYY
                     YGTFDFEDTVIGRDYRALVGVYAERNGGGDLRSVYTNEVTAKR"
     gene            complement(2272..>2583)
                     /locus_tag="ACZPAQ_06645"
     CDS             complement(2272..>2583)
                     /locus_tag="ACZPAQ_06645"
                     /inference="COORDINATES: protein motif:HMM:NF025643.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HIR0903700.1"
                     /translation="ESLGVTTNVDPDFIRIAETYSYDEKKIKIMIRRYNSEEALDKVS
                     VEKEDEQQDVKYIRGGVEHSVAQNRSIYTASWTNGLNVVSIIGDMTEEELEQMIDSIY
                     T"
BASE COUNT          454 a          844 c          742 g          543 t
ORIGIN      
        1 gctgcgacta ctccatcgag cccaaccggg gggaggccat ccgccaggcg gtgctgggct
       61 gccgggagcc ctccgtcctc ctcatcaccg gcaaaggggc agagacccgc cagaagcggg
      121 gcaccgagta tatcgacacc ccctctgacg tggactatgt ccagtccttt cttcaggagt
      181 acgacgtgcg ccacggcctg gacggcatgg agaaggtgcg ctccctgctg tccattctgc
      241 ccatcctcaa gcgggacgag ggccggaccg ttgtggtgaa atacggcggc tccgccctgg
      301 gggcggaggc ggccaccgac accaccctcc aggacgtggc cgccctgcgc atggtgggcg
      361 tccgggtggt gctggtccac ggcggcggca agcacatcac cgccctgctg gacaagctcc
      421 aggtgcccac ccgtttcgag aacggctacc gctataccga ccaggcggtg ctggagacgg
      481 cggaaatggc cctctccgcc caggtaaaca aggccatcgt ctcccgtctg agccagctgg
      541 aggtgtctgc cgtaggcctg tccggcaagg acggcggcct gctcaccgct gtggagaagg
      601 acccggccct gggccgggtg gggtccatca cccgggtgga gccccgtatc cttcagaccc
      661 tgctggacgg ggacttcctg ccggtggtct cccccatcgc cgcgggggag gacggcggcg
      721 gcttcaactg caatgccgac gacgcggccc gggcggtggc ggcggctctg ggggcggaca
      781 agctgatctt cctcaccgac accgccgggg tgcttatcga cagccacaac agcaagactg
      841 ccgtccccca catggatgtg aagcgggcgg aggagctgat cgacaccggg ctcattgccg
      901 ggggcatggt gcccaaggtg cggggctgta tccaggccat ccgcgccggg gtgggcgagg
      961 tgtccatcct ggacggccgg gtggagcacg ccctgctgct ggagatgctc aaccagcggg
     1021 tccaggggac caccatcacc ggctgacccc cgttcaaaag aaaacggggc tgtcttttcc
     1081 gctttttcca caagctgaaa cggcgccccc gcctgtggcg ggggcgccgt ctatttcaat
     1141 tttcaaggtc cggctggcgg gtggaggtcc gccggccggg ccggttttca ttggctctgt
     1201 gctctcttta caggatcgcg tccaggatca cgtcttccag ctggaggggg tccagctggc
     1261 cccggttgca ccgatcggcc agccggtcca tctctccccg gtcaatggag acgtcccggt
     1321 actgcgctac cggcggcgcg tctttcctgt cccgccggta gcagtcgatc ccgtacacct
     1381 gccgtgcccc tgcctcctga aaggtcatct ggcactcatt cgggcggtag agatactgca
     1441 tgagtccctc tcacctcgct tttcaacaca tctaaactga tatttgattc gctggcgata
     1501 tggatcaccg tggacagctt ggggttgacc cggccggcct ccatgtccag atagtgccgg
     1561 ggagacaccc cgcaccgctc ggccgcttcc tcctgggtca gctcgttctc cctcctccag
     1621 gtgtacagga gcctggaaaa gcgccatttc agcgccatca gctccccgcc ggtctggagg
     1681 atcagcccgt cctccggccc gcacccgtct gccacagaag cagcacagac aggaccagaa
     1741 cggtctgtcc gctccacctc cgcgtcacca gcggggcgag tccgctccat atggtgtacc
     1801 atccgtcatc ttttggccgt cacctcgttg gtgtagacgg agcggaggtc cccgccgccg
     1861 ttccgctcgg cgtacacacc aaccagggca cggtagtccc gtccgatgac cgtatcctca
     1921 aaatcgaagg tcccgtaata atagtccgtg ttctcctcat acatattcaa atcttctttg
     1981 gtgaaggtct ccaaagtggt ccaggtgtct cctccgtccc tggactcctg cacggtgata
     2041 tagctgaccc ccacctcatc ggaggtacgg actgcgtcca catccgcgtg gattcggacc
     2101 acgccgtcgc tcttcgcggt ggcccacaca ctgtatatgg ccaaatagtc gctggcacgg
     2161 gcaaaggcgg tggtcatcag caatccggcc agcatggtac aggccagcag cacggcgcag
     2221 ccgcgggaaa tcttcttttt catcggttct ctttcctctc tgttctcttt tctaagtata
     2281 gatggagtcg atcatctgct ccagctcctc ctccgtcata tctcctataa tcgatactac
     2341 attaagtcca ttggtccatg acgcggtata aatcgatctg ttttgtgcaa cagaatgttc
     2401 cacaccacct cggatgtatt ttacatcttg ttgctcgtct tctttttcaa cagatacttt
     2461 atcaagcgct tcctctgaat tataccggcg aatcattatc ttaatttttt tctcatcata
     2521 actgtatgtc tctgcaattc taataaaatc cgggtctaca ttggtcgtaa ctcccagact
     2581 ttc
//
LOCUS       DBMDSJ010000255         2574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9594_length_2574_cov_1.403154, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000255 DBMDSJ010000000
VERSION     DBMDSJ010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9594_length_2574_cov_1.403154"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /gene="dapB"
                     /locus_tag="ACZPAQ_06650"
     CDS             <1..543
                     /gene="dapB"
                     /locus_tag="ACZPAQ_06650"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR0903701.1"
                     /translation="LDFARATGTPLVLATTGYTPEQVAQIGAAALEVPIFRSANMSLG
                     INVLLELVKKAASVLGDSYDIEIVERHHRRKVDAPSGTALMIADAAAGACGHETEYVF
                     ERHSVRHPRDKKEIGISAVRGGTIVGEHEIIFAGHDEVMEIRHTALSREIFAQGAVEA
                     AKFMAGVKAPGLYDMSSLIR"
     gene            646..1008
                     /locus_tag="ACZPAQ_06655"
     CDS             646..1008
                     /locus_tag="ACZPAQ_06655"
                     /inference="COORDINATES: protein motif:HMM:NF022158.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 family protein"
                     /protein_id="HIR0903702.1"
                     /translation="MAVAAAVLAALCISLIIFRLHNLQDSSRGEPVRAEEARVHAKEN
                     CHSRAGSSYYLYFHVPCRDTVVTCQVPPDIWYYLSKGDRGTLCHQGGRFISFTRDGEL
                     IAVDPVAERHQLWRIHGS"
     gene            1122..1490
                     /locus_tag="ACZPAQ_06660"
     CDS             1122..1490
                     /locus_tag="ACZPAQ_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HIR0903703.1"
                     /translation="MKFYICEHCGNIVTFLQSSGVPVMCCGQKMTELVPDSTDAAQEK
                     HVPVVTVEGSMVDVKVGSAPHPMIPEHYIQWVALETTEGCQIKYLEPGQAPEVKFALA
                     PGDRVKAAYAYCNLHGLWRA"
     gene            complement(1552..2349)
                     /locus_tag="ACZPAQ_06665"
     CDS             complement(1552..2349)
                     /locus_tag="ACZPAQ_06665"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR0903704.1"
                     /translation="MELKLSQEFLTENDCYQAGRKIVPKGIMVHSTGVAQPDPQVFIR
                     RWNKPGVEVCVHAFVARDEVIQTLPWNWRGWHAGSGKNGSANNTHISFECCEPAGHTY
                     QNGKMVGYDVEKNEAYFQDIYRNAVELSAMLCRQYRLDPMEPGVILDHAGGHALGIAS
                     NHSDVGQWWPLHGKTMDDFRRAVRDAMRGGEETVTQEQFDAMMADYLARRGREKPSDW
                     SENARRWAEEAGLVSGDSAGMRYRDFTTREETIQFLYRYQQVTEGKP"
BASE COUNT          453 a          844 c          746 g          531 t
ORIGIN      
        1 ctggacttcg cccgggccac cgggacccct ctggtgctgg ccaccaccgg ctacacccct
       61 gagcaggtgg cccaaatcgg cgccgctgcc ctggaggtgc ccattttccg ctccgccaac
      121 atgtccctgg ggatcaatgt actgctggag ctggtgaaaa aggcggcctc tgtgctgggg
      181 gacagctatg acatcgagat cgtggagcgc catcaccgcc ggaaggtgga cgctcccagc
      241 ggcactgccc tgatgatcgc cgacgcggcg gcgggggcct gcggccacga gacggagtac
      301 gtcttcgagc gccacagcgt ccgccatccc cgtgacaaga aggagatcgg catctccgcg
      361 gtccggggcg gcaccatcgt gggggagcac gagatcatct ttgccggcca cgacgaggtc
      421 atggagatcc gccacactgc cctctcccgg gagatcttcg cccagggcgc cgtggaggcg
      481 gccaagttta tggccggtgt gaaggcaccc ggcctgtacg atatgagcag cctcatccgg
      541 taagtcagaa aaaacagcct tccactccgg tggaaggttg ttttttgcgc ttttttctgt
      601 tatgctggtc ttgtacctca ggcctttgga ggaaggaggc aggatatggc tgtcgctgcg
      661 gcagtactgg ccgccctgtg catcagtttg atcatcttcc gtctgcacaa ccttcaggac
      721 tcctccagag gggagcctgt ccgggccgaa gaggcccggg tccacgccaa agagaactgc
      781 cactcccgcg ctggctcctc ctactacctc tatttccatg tcccctgccg ggacactgtg
      841 gtgacctgcc aggtcccgcc ggacatctgg tactatctct ccaaggggga ccggggcacc
      901 ctgtgccatc agggcgggcg gttcatctcc ttcacccggg acggggagct gatcgccgtg
      961 gacccggtgg cggagcggca tcagctgtgg cggatacacg gctcgtaaca ctaagattct
     1021 gttgctattt ccctgtattt tgggtataat agggctgagc tcttccggca catcccggcc
     1081 ggaaaatcac gggctcccca ttgcaaagga ggcagcatcc tatgaagttc tacatctgtg
     1141 agcactgcgg gaatatcgtc acctttctcc agtccagcgg cgtgcccgtc atgtgctgcg
     1201 ggcagaagat gaccgaactc gtccccgatt ccaccgacgc ggctcaggag aagcatgtcc
     1261 ctgtggtcac cgtagaaggc agcatggtgg atgtcaaggt gggctccgcc ccccatccca
     1321 tgatccccga gcactatatc cagtgggtgg ccctggagac caccgagggc tgtcaaatca
     1381 aatacctgga gccaggtcag gccccggagg tcaaatttgc cctcgccccc ggcgaccggg
     1441 tcaaggccgc ttacgcctat tgcaatcttc acgggctctg gagagcctga gcgacaccat
     1501 gaccagaagc cccctctgcc ggctcttccg gcggaggggg ctctttctcc gttatggctt
     1561 cccttccgtc acctgctggt agcggtaaag gaactggatg gtctcctccc gggtggtgaa
     1621 gtcccgatag cgcatcccag cgctgtcccc ggagaccagt ccggcctcct ccgcccagcg
     1681 gcgggcgttc tcagaccagt cagagggctt ctctcggcct ctgcgggcca gataatccgc
     1741 catcatggca tcaaactgtt cctgtgtcac cgtctcctct cctcctctca tcgcatccct
     1801 cacggcccgc ctgaagtcat ccatggtctt cccgtgcagc ggccaccact ggcccacatc
     1861 gctgtggttg ctggcgatcc ccagggcgtg gccgccggcg tggtccagga tcacgcccgg
     1921 ctccatgggg tccaggcggt actggcggca cagcatggcg gacagctcca cggcgttgcg
     1981 gtagatgtcc tgaaaatatg cctcgttctt ctccacatca tagcccacca tctttccgtt
     2041 ctgataggtg tgcccggctg gctcacagca ctcaaaggag atgtgggtat tgttggcgct
     2101 gccgtttttt cccgagcctg cgtgccagcc ccgccagttc cagggcaggg tctggatgac
     2161 ctcatcccgg gccacgaagg cgtggacgca cacctccacc ccaggcttgt tccagcggcg
     2221 gatgaacacc tgcgggtccg gctgcgccac gccggtggag tggaccatga tccccttggg
     2281 tacgatcttc cgcccggcct ggtaacagtc attctctgtc aggaactcct gactcagctt
     2341 cagttccatg gctccctcct ctgtccatac aaaggctgtt ccatcctatg ccgcctcctt
     2401 ttctattgac accctctccc aactcctcta aaatagaaag gtcaaaagag aaagaaaaag
     2461 ggggtccctt cctatgggag aactcaatcc ggtgcagaag cggggcaatg ccgccgccct
     2521 gctccccatc ctagtgttcc tggtcctcta tttgggctgc ggcctgctgt tcga
//
LOCUS       DBMDSJ010000256         2565 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9641_length_2565_cov_1.343344, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000256 DBMDSJ010000000
VERSION     DBMDSJ010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9641_length_2565_cov_1.343344"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="ACZPAQ_06670"
     CDS             <1..991
                     /locus_tag="ACZPAQ_06670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR0903705.1"
                     /translation="VLSKYRSELMGAAMLWVMLFHAADLTFPIPGLDLFRAAGFGGVD
                     IFILLSAMGLSLSLSRREQEYGAFLVRRARRILPAYFVVMVPYTLFLILRGQAPLSAL
                     VWNSTLLAYWVHTPGAFNWYVSGILLFYVLTPWCFRRLRRSRHRVLWTAGAGAAAVAV
                     CHVLIQDEYWHYLDVFYRFPIFFLGLLMGLYVWEDRRLGRRDLLFWTLWLAAGLCYLA
                     AALPRLEVPHLPMCHLFLFTTVPMCLAGCACFEHLPLGWLRRGLRLVGEHSLEIYLLN
                     VSLFSETALLRSWLPLGTGPYYFLAFFLNILLGILLHRGVEAGMRRIQARRAD"
     gene            1012..1965
                     /locus_tag="ACZPAQ_06675"
     CDS             1012..1965
                     /locus_tag="ACZPAQ_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025755.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HIR0903706.1"
                     /translation="MGKIKRLLDRFDEMGRYPIQELGYSRTWLMFSYLGAFLVHRCTI
                     ADYFLYHFYERNRRGRAEFVTLYDMRDIHRKNPQTAFADFEEKDRFLDRFQAYVHRDW
                     IGRVCRNSREEFDAFADRHPRCIIKPRTDSGGHGVELCTITPNNRDQVWERLTSQDMI
                     AEELLEQCPEMAALHPSSVNTVRALTIRGKLCSAVLRMGVGGGFVDNGCSGGIFAGLD
                     VDTGIVTTQGADLMEHRFLLHPTTGLTIPGFQVPQWEQVRRTVEEAVTLVPNAIIVGW
                     DVAVTAQGPVLIEGNAYPSVQIMQTAGMQGLKRLWNSALRG"
     gene            2124..>2565
                     /locus_tag="ACZPAQ_06680"
     CDS             2124..>2565
                     /locus_tag="ACZPAQ_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR0903707.1"
                     /translation="MYRSITLPNGAKLLTEFVPGARTAALGFFVGTGSRHERAAESGA
                     AHFIEHMSFKGTQRRDAGALARETDAIGGQINAYTTKELTCYYARCLDSHLHRAIDLL
                     SDMLFHSRFAQGDVELERGVILEEIGMYEDTPEDLAAERLAAAVY"
BASE COUNT          380 a          869 c          788 g          528 t
ORIGIN      
        1 tgtgttaagc aaatatcgct ctgagctgat gggggccgcc atgctctggg tgatgctgtt
       61 ccatgccgct gatctgacct tccccatccc cgggctggac ctgtttcggg cggcgggctt
      121 cggcggggtg gacatcttca tcctgctctc cgccatgggg ctgtccctct ccctgagccg
      181 ccgggagcag gaatacggtg cctttctggt gcgccgggcc cggcgcatcc tccccgccta
      241 ttttgtggtg atggttccct acaccctctt cctcattctt cggggacagg cccccctctc
      301 cgccctggtg tggaactcca ccctcctcgc ctactgggtc catacccctg gggccttcaa
      361 ctggtatgtg tccggcatcc tgctcttcta tgtcctcacc ccctggtgtt tccgccggct
      421 gcgccgctcc aggcaccggg tgctgtggac ggcaggggcc ggcgccgcgg cggtggctgt
      481 gtgccacgtg ctgatccagg acgagtactg gcactacctg gacgtctttt accgcttccc
      541 catctttttt ctgggactgc tgatggggct ctatgtgtgg gaggaccgcc ggctgggcag
      601 gcgtgatctg ctgttctgga ccctgtggct ggcggccggc ctgtgctacc tggccgcggc
      661 cctgccccgg ctggaggtcc cccacctccc catgtgccat ctgtttctgt tcaccaccgt
      721 ccccatgtgc ctggcgggct gcgcctgctt tgaacacctg cccctgggat ggctgcgccg
      781 gggactgcgg ctggtggggg agcacagtct ggagatctat ctgctcaacg tcagcctctt
      841 ctccgagact gccctgctgc gcagctggct ccctctgggg actggcccct actatttcct
      901 cgcctttttc ctgaatatcc ttttgggcat cctgctccac cggggggtgg aggccgggat
      961 gcgccgcatc caggcccgcc gggccgattg atccgcaaag gaggccgcac tatgggaaag
     1021 atcaaacgtc tgctggaccg cttcgacgag atgggccgct accccatcca ggagctgggc
     1081 tactcccgca catggctgat gttcagctat ctgggggcct ttctggtcca ccgctgcacc
     1141 atcgccgact acttcctcta ccacttctat gagcgcaacc gccggggccg ggcagaattc
     1201 gtcaccctgt acgacatgcg ggatatccac cgcaagaacc cccagaccgc cttcgccgac
     1261 tttgaggaga aggaccgctt tctggaccgg ttccaggcct atgtgcaccg ggactggatc
     1321 ggccgggtgt gccggaacag ccgggaggaa ttcgacgcct ttgcggaccg ccatccccgc
     1381 tgcatcatca agccccgcac tgactccggc ggccacggcg tggagctgtg caccatcact
     1441 ccaaacaacc gggatcaggt gtgggagcgg ctcacctccc aggatatgat cgccgaggag
     1501 ctgctggagc agtgcccgga gatggccgcc ctccacccca gctcggtgaa tacagtccgg
     1561 gccctcacca tccggggaaa gctttgctcc gccgtcctgc gcatgggggt gggcggcggc
     1621 tttgtggaca acggctgctc cggaggcatt ttcgccggcc tggacgtgga caccggcatc
     1681 gtcaccaccc agggggccga tctgatggag caccgcttcc tcctccaccc caccaccggc
     1741 ctgactatcc ccggcttcca ggtcccccag tgggaacagg tgcgccggac ggtggaggag
     1801 gcggtgaccc tggtccccaa cgccatcatc gtgggctggg acgtggccgt caccgcccag
     1861 ggtcctgtgc ttatcgaggg caatgcctat cccagcgtgc agatcatgca gacagccggg
     1921 atgcagggac tcaagcgcct gtggaacagc gccctgcggg gctgaccgcc ttctgcccgg
     1981 gcgcggaggc cgaattttcg gcccccgcgc ccttcttttc gtcttttttc ttgattttcc
     2041 gaccattctg ttatagactg tctaagactt gccaggatag gtggctgcct cccccgcagt
     2101 caatcttttc gaaggagaga tctatgtacc gttccattac cctgcccaac ggggcaaagc
     2161 tgctgaccga attcgtcccc ggagcccgca ccgcggccct ggggttcttt gtgggcaccg
     2221 gctcccgcca cgagcgcgcc gcggagagcg gcgcggccca ctttattgag cacatgtcct
     2281 tcaaggggac ccagcggagg gacgctggcg ctctggcccg ggagaccgac gccatcggcg
     2341 ggcagatcaa cgcctatacc actaaggagc tcacctgtta ctatgcccgg tgtctggaca
     2401 gccacctgca ccgggccatc gacctgctca gcgacatgct gttccactcc cgttttgctc
     2461 agggggacgt agagctggag cgtggggtca ttctggagga gatcgggatg tatgaggata
     2521 ccccggagga cctggccgcg gagcggctgg ccgccgcggt ctacc
//
LOCUS       DBMDSJ010000257         2561 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9652_length_2561_cov_1.597154, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000257 DBMDSJ010000000
VERSION     DBMDSJ010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9652_length_2561_cov_1.597154"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="ACZPAQ_06685"
     CDS             <1..574
                     /locus_tag="ACZPAQ_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR0903708.1"
                     /translation="KISSFKKEQDCRIAHVALLPERPAALPAALQAIREADLILMGPG
                     SLYTSVIPNLLVEGVVEAICRSEALKIYVCNIMTQEGETEGYTAADHVDALLSHGAPG
                     LVDLCLANSAPVRPGLVEKYREEDAAPILVDRERIRAMGLELEEYPVASEGGDYARHD
                     PDRLAAAVLDVYRRRAVRIFRGEQRYIIEE"
     gene            579..1499
                     /gene="whiA"
                     /locus_tag="ACZPAQ_06690"
     CDS             579..1499
                     /gene="whiA"
                     /locus_tag="ACZPAQ_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR0903709.1"
                     /translation="MSFAGETKGELCRTALNRKCCTQAEAYGVLLYCNTFSGREIRII
                     TENEAFAQRLPTLFRKAFRLSFDRLPDGEGKYSFGITDPAKLAAVSGTFGMDAGSLAN
                     HVNFAVLEEPCCRAAFLRGAFLAGGSVTDPRKAYHLELSTSHYNVSREVPALMREAGF
                     QPKEATRKANYIAYFKQSERIEDFLTAIGAPLAAMEIMNAKLEKNLRGSVNRRVNCDA
                     ANLDKAVEAAQVQLEAIRSLEQSGQLEGLPDKLRETARLRLEHPEDTLAELAALCDPP
                     ATKSALNHRLRKLVELGQARGEGAGPGKTI"
     gene            1517..2143
                     /locus_tag="ACZPAQ_06695"
     CDS             1517..2143
                     /locus_tag="ACZPAQ_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016763.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR0903710.1"
                     /translation="MTLICYLTAGAVNLFWLILGDRLPLPVQMAIPILTVLLAGTGVL
                     LRLRKLRGEQRRRYRTGALWVLLLYYLCILSVLLFFGGLFHLDRGYGGAVNLEPFHTI
                     RNYIIYYRRTGSFVSVTNLLGNVVILVPLGVLMPVMFRPMRHFWTFLPLAALVSCGVE
                     YIQWVTATGAADVDDSILNFIGAALGYLFTRLCQITAGMIAKRRSRGE"
     gene            2211..2561
                     /locus_tag="ACZPAQ_06700"
     CDS             2211..2561
                     /locus_tag="ACZPAQ_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HIR0903711.1"
                     /translation="MEQEKVFAMKLSKIYPLLTAKAERKGRTRQEVDEVICWLTGYDR
                     AGLERQLSLDIDYGTFFREAPRMNPSCGKITGTVCGVRVEEIEDPLMRKIRYLDKLID
                     ELAKGKQLEKVLRK"
BASE COUNT          466 a          753 c          909 g          433 t
ORIGIN      
        1 caagatcagt tcctttaaaa aagagcagga ctgccgcatc gcccatgtgg cactgctccc
       61 ggagcggccc gccgccctcc cggccgccct ccaggccatt cgggaggcgg acctgatcct
      121 gatgggaccg ggcagcctgt acaccagcgt catccccaac ctgctggtgg agggggtggt
      181 ggaggccatc tgccgctccg aggcgctgaa gatctatgtg tgcaacatca tgacccagga
      241 gggggagaca gaggggtaca ccgccgccga ccatgtggac gccctgctgt cccacggggc
      301 gccggggctg gtggacctgt gcctggccaa ctcggcgccg gtgcgtcccg gcctggtgga
      361 gaagtaccgg gaagaagacg ccgcccccat cctggtggac cgggagcgca tccgggccat
      421 ggggctggag ctggaggagt atccggtggc ctcggagggg ggcgactatg cccgccacga
      481 cccggaccgg ctggcggcgg cggtgctgga tgtgtaccgc cggcgggcgg tgcgcatctt
      541 ccggggcgaa cagcggtaca tcattgagga gtgagaccat gtcctttgca ggagagacca
      601 agggggagct gtgccggacg gcgctgaacc ggaaatgctg cacccaggcg gaggcatacg
      661 gcgttctgct ttactgcaac accttttccg ggcgggagat ccgcatcatc acggagaacg
      721 aggcatttgc ccagcggctc cctaccctgt tccgcaaggc gttccgcctc tcctttgacc
      781 gtctgcctga tggggagggg aagtacagct tcggcatcac ggaccctgcc aagctggcag
      841 cggtgagcgg gaccttcggg atggatgcgg gcagtctggc aaaccacgtg aacttcgccg
      901 tgctggagga gccctgctgc cgggctgcct tcctccgggg ggcgttcctg gcgggagggt
      961 cggtcaccga cccccggaag gcctatcacc tggagctgtc cacctcccac tacaacgtga
     1021 gccgggaggt gcccgccctg atgcgggagg cgggcttcca gcccaaggag gccacccgga
     1081 aggccaacta tatcgcctat ttcaagcaga gtgagcgcat cgaggacttc cttaccgcca
     1141 tcggcgcgcc cctggccgcc atggagatca tgaacgccaa gctggagaag aacctgcggg
     1201 gcagcgtgaa ccggcgggtg aactgtgacg cggccaacct ggacaaggcg gtggaggcgg
     1261 cccaggtcca gctggaggcg atccggagcc tggagcagtc cgggcagctg gagggcctgc
     1321 cggacaagct gcgggagacg gcccgactgc ggctggagca cccggaggac actctggcgg
     1381 agctggcggc cctgtgtgac ccgccggcga ccaagtcggc cctgaaccac cggctgcgta
     1441 agctggtgga gctgggacag gcccggggcg agggggcggg gcccggcaaa actatctgat
     1501 accacgggag agatctatga ccttgatttg ttatctgacc gccggagccg tcaacctgtt
     1561 ctggctgatc ctgggggaca ggctcccgct cccggtgcag atggcgatcc ccatcctgac
     1621 cgtgctgctg gcagggaccg gggtgctgct gcgtctgagg aagctgcggg gagagcagcg
     1681 gcggcgttac cgcacggggg ccctgtgggt cctgctgctc tactacctgt gcatcctgtc
     1741 ggtgctgctg tttttcggcg ggctgttcca cctggaccgg ggctatggcg gggctgtgaa
     1801 tctggagccc ttccacacca tccgaaacta tatcatctac taccggcgga cgggcagctt
     1861 tgtcagcgtg accaatctgc tgggcaatgt ggtgattttg gtgccgctgg gggtgctgat
     1921 gccggtgatg ttccggccta tgcgccactt ctggaccttt ctgcccctgg ccgcgctggt
     1981 gtcctgcggc gtggagtaca tccagtgggt gaccgccacc ggcgcggcag atgtggacga
     2041 ctccatcctg aactttatcg gcgcggccct gggatacctg tttacccggc tgtgtcagat
     2101 cacagcgggc atgatagcaa agcggaggag ccgcggggaa taaatggagg cctttggacg
     2161 atatgccatc tggcagggga gacccttgaa aaaggcggcg gagggatgat atggaacagg
     2221 aaaaagtatt cgccatgaag ctgtcgaaga tatatccact gctgacggcc aaagcggagc
     2281 ggaagggccg cacccggcag gaggtggatg aggtcatctg ctggctgacc ggatacgacc
     2341 gggcgggcct ggagcggcag ctcagcctgg acatagacta tgggaccttt ttccgggaag
     2401 cgccccggat gaaccccagc tgcgggaaga tcacagggac cgtctgcggc gtccgggtgg
     2461 aggagatcga ggacccactg atgcggaaga tccggtattt ggacaagctg atcgacgagc
     2521 tggcaaaggg aaagcagctg gagaaggtcc ttcggaaata g
//
LOCUS       DBMDSJ010000258         2557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9670_length_2557_cov_1.645358, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000258 DBMDSJ010000000
VERSION     DBMDSJ010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9670_length_2557_cov_1.645358"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="ACZPAQ_06705"
     CDS             <1..455
                     /locus_tag="ACZPAQ_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903712.1"
                     /translation="PFQDNAPVTVAVVQTAGGLDLVPISDVCCPPTGALSNFRMSNLA
                     YNSQPLDLLLADGRVVYTDVQFKETTYFKQIRPGTYQFYLAETNLLPMPSYLDIETMD
                     SAFIGVAPPFSALASLQLEAAPRSTYTLYVLSGGSGSNEIQTLVVTDR"
     gene            complement(562..1824)
                     /locus_tag="ACZPAQ_06710"
     CDS             complement(562..1824)
                     /locus_tag="ACZPAQ_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR0903713.1"
                     /translation="MAYRPLADEIRPQTLDEVVGQKHILGREGLLRRIIEGGDIPNLI
                     FYGPSGTGKTTVANIIARRTNRPLHRLNATTASISDIKEIIADIGTLMAPEGVLLYLD
                     EIQYFNKKQQQSLLEFMENGKITLIASTTENPFFYVFGAVLSRSTVFEFKPITAEDAL
                     PAIDRGVSILEGRLGGGLVLEEGVREHIAASCGGDIRKAMNALELLASAAKREQGKFL
                     VTLSDAQTAAQKSAMRYDREGDNHFDIASALMKSMRGSDPDAAVHYLARLLEAGDLIT
                     AIRRILCSASEDIGLAYPQAVPIVKACVDSALQLGLPEARLPLAQAAILLATAPKSNT
                     ACMAIDAAMADVKAGLAGDIPRELQNVHADSAGMEREQGYLYPHNFPGHWVRQQYLPN
                     ELKDRKYYQYGDNKTEQAARRYWEEIKK"
     gene            complement(1873..2337)
                     /locus_tag="ACZPAQ_06715"
     CDS             complement(1873..2337)
                     /locus_tag="ACZPAQ_06715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903714.1"
                     /translation="MSGKGSEARLTHGGDPRFRELVRAMDREYGERFGAAALKCRSCH
                     GLDDIRQACLLLRDGQVVACGAFRAMEEGTAELNWVYVRPEHRRRGCARQVVEVLELQ
                     ALFQGFGRMVMEIGQDIPEAISLYRKMGYREIETLTIFADNKDTLCMEKELL"
     gene            complement(2353..>2557)
                     /locus_tag="ACZPAQ_06720"
     CDS             complement(2353..>2557)
                     /locus_tag="ACZPAQ_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903715.1"
                     /translation="MGRDQGFAKNVFDLGCVAATVCLGLLLTGRIVGIGLGTLLAMVG
                     VGRVIALVNHWTKAGMSRLAGVA"
BASE COUNT          437 a          908 c          711 g          501 t
ORIGIN      
        1 tgcccttcca ggacaacgcc cctgtcaccg tagcagtggt ccagaccgcc ggcggcctgg
       61 acctggtccc catctccgat gtgtgctgcc cccccaccgg cgctctgtcc aacttccgga
      121 tgagcaacct ggcctacaac agccagcccc tggacctcct tctggccgac ggccgggtgg
      181 tctatactga cgtgcaattc aaggagacca cctatttcaa gcagatccgc cccgggacct
      241 accagttcta tctggcggag accaaccttc tccccatgcc cagctacctg gacatcgaaa
      301 ccatggactc cgccttcatc ggggtggcac cccccttctc tgccttggcc tccctccagc
      361 tggaggcggc gcccaggtcc acctacaccc tgtatgtcct cagcgggggc tccggctcca
      421 acgagatcca gaccctggtg gtgacagacc gctgactctc ccggcagaca aaggtcctgc
      481 ctttccagta aaggccccgc gggcggatgc tatccgcccg cggggccttt ggcctcaaca
      541 gcgcccgctg gaggcggtct cctatttctt gatctcctcc cagtagcgcc gggcggcctg
      601 ctctgtcttg ttgtccccgt actgataata ttttctgtct ttcagctcat tgggcaggta
      661 ctgctgccgc acccagtggc ccgggaagtt atgggggtag aggtagccct gctcccgctc
      721 catccccgcc gagtcggcat ggacattctg gagctcccgt gggatgtccc ccgccagtcc
      781 tgccttcacg tcggccatag ccgcgtcaat ggccatgcag gccgtgttgg acttgggagc
      841 ggttgccaga aggatggccg cctgggccag cggcagcctt gcctcaggca gccccagctg
      901 aagggcgctg tccacgcagg cctttacgat gggcaccgcc tggggatagg ccaggccgat
      961 gtcctcactg gcagagcaga ggatgcggcg gatggccgtg atcaggtccc ccgcctccag
     1021 cagccgggcc agataatgga ccgccgcatc cgggtctgaa cctcgcatag atttcatcag
     1081 ggcggaggca atatcaaagt gattgtcccc ctcccgatca taccgcatag cggacttctg
     1141 ggccgccgtc tgggcgtctg acaaagtgac aaggaatttt ccttgctccc ttttcgccgc
     1201 cgaggccagc agctccaggg cgttcatggc ctttcggatg tcaccgccgc aggaggcagc
     1261 aatgtgctcc cgcaccccct cctccagcac caatccgccc cccaggcgcc cctccaggat
     1321 ggacaccccc cggtcgatgg cgggcagggc gtcctccgcg gtgatgggct tgaactcgaa
     1381 cacggtggac cgggagagca ctgccccgaa cacgtagaaa aaggggttct ccgtggtgga
     1441 ggcaatgagg gtgatcttgc cgttctccat gaactccagc agggactgct gctgcttctt
     1501 gttgaaatac tggatctcat ccaggtaaag gagaactcct tccggggcca tcagggtccc
     1561 gatgtctgcg atgatctctt tgatgtcact gatggacgcg gtagtggcgt tcagccggtg
     1621 caggggccgg ttggtccgcc gggcgatgat gttggccacc gtggtctttc cggtacccga
     1681 gggaccgtaa aagatcaggt tggggatgtc acccccctcg atgatccggc ggagcaggcc
     1741 ctccctgcct aaaatatgct tctgccccac cacttcatcc agggtctggg gccgtatctc
     1801 atctgccaat ggccggtaag ccattccaac actccctttc cctccagggg cgcccgcccc
     1861 tggacccgcc gctcacagca gctccttttc catgcacagg gtgtccttgt tgtccgcaaa
     1921 gatggtgaga gtctcgatct cccggtatcc catttttctg taaagggata tcgcttccgg
     1981 gatatcctgg cctatctcca tcaccatgcg gccaaagccc tgaaacaggg cctggagctc
     2041 caacacctcc accacctgcc tggcacaccc ccggcggcgg tgttctggac ggacatagac
     2101 ccaattcagt tccgctgtgc cctcctccat cgcccggaag gcaccgcagg ccaccacctg
     2161 cccgtcccgc aggagcaggc aggcctgccg gatgtcatcc aggccgtggc agctccggca
     2221 cttcagggcc gctgccccaa accgctcccc atactcccgg tccatcgccc ggaccagctc
     2281 ccggaagcgc gggtccccgc cgtgggtcag ccgggcctct gatccttttc cgctcatttc
     2341 ttgtcctccc gtttatgcta cgccggccag ccggctcatg cccgctttgg tccaatggtt
     2401 taccagggcg atcacccgcc caacccccac catggccagc agagtgccca ggccgatccc
     2461 cacgatccgc ccggtcagca gcaggcccag gcacaccgtt gctgccacgc agcccaaatc
     2521 aaatacgttc ttggcaaagc cctgatcccg gcccatc
//
LOCUS       DBMDSJ010000259         2537 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9754_length_2537_cov_1.794335, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000259 DBMDSJ010000000
VERSION     DBMDSJ010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9754_length_2537_cov_1.794335"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            271..942
                     /gene="rpe"
                     /locus_tag="ACZPAQ_06725"
     CDS             271..942
                     /gene="rpe"
                     /locus_tag="ACZPAQ_06725"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR0903716.1"
                     /translation="MIKVSPSILSADFVNLERDIRRVSDADYLHVDVMDGAFVPNLTI
                     GVPVVQSIRRCTDMFLDVHLMIEKPVRYIESFAKAGADLLSIHLEADHPTRIAQALRV
                     MGECGVKKAVALRPITKAEAILPYLEELDLVLVMTVEPGFGGQAFMESQLDTIRQVRA
                     LIERYNPACELEVDGGISPKTAPLVVEAGANVLVAGSAVYGAEDIPAAIQALRVSGGV
                     RGERV"
     gene            929..1096
                     /locus_tag="ACZPAQ_06730"
     CDS             929..1096
                     /locus_tag="ACZPAQ_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HIR0903717.1"
                     /translation="MREYKILSADSLEQAEQVMNDMALAGWRTTSLTPYPAGFPAGVS
                     YLFAVVLERER"
     gene            1147..1749
                     /gene="recR"
                     /locus_tag="ACZPAQ_06735"
     CDS             1147..1749
                     /gene="recR"
                     /locus_tag="ACZPAQ_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR0903718.1"
                     /translation="MQYFPPALENLVEQFARLPGVGVKSAQRLAFHVLSLPEEDAAAF
                     AQAILSAKSAIHCCPVCQNLTEGDGPCSICASPKRDASVICVVADPKDVVAMERAREY
                     QGLYHVLHGVISPMNHVGPDDLHIKSLVERVAAGGVQEVIMATNPDTEGEATAMYLSR
                     LLRPFGVKITRLAYGIPVGSHLEYADDATLMRALEGRQEM"
     gene            2100..>2537
                     /locus_tag="ACZPAQ_06740"
     CDS             2100..>2537
                     /locus_tag="ACZPAQ_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0903719.1"
                     /translation="MVNQRMLGLGTARSVIRELFEYGKIRAAQVGAENVFDFSLGNPS
                     VPCPQEVNDTAVRILREQPEVIHCYTSAQGDQEARQRFADSLNRRFGTCYTADQFYIT
                     VGAAASLCCVFNGLTCPGDEFVVFAPYFPEYKVFIEGAGAKMVL"
BASE COUNT          410 a          857 c          778 g          492 t
ORIGIN      
        1 tccgccccgc cggcgcacag ccactgcccc cgccgggcag agcccggcgg ggggtatctg
       61 aaaagcggcg ggccatccgg atcggccggg gccgccgggc ggccgtggga tcagcctgga
      121 gcaggcctcg ggtccggggc tccggggagt gcttccgccc tcccgctctc cgctctgcat
      181 cctatgcgtc ggaccggccc tctgcccctg gcccagcccc tacagccctg atcaaaacct
      241 ctcatttttt cggaaagcgg ggtcaaaacc atgataaaag tttcaccttc catcctctcc
      301 gccgattttg tcaatctgga acgggatatc cggcgggttt ccgatgccga ctacctccat
      361 gtggacgtga tggacggcgc tttcgttccc aatctcacca tcggcgtccc ggtggtccag
      421 tccatccgcc ggtgcaccga catgttcctg gatgtccatc tgatgatcga aaagccggtg
      481 cggtacatcg aatcctttgc caaggctggg gcggacctgc tctctatcca cctggaggcc
      541 gaccacccca cccgcatcgc ccaggctctg agggtcatgg gggagtgcgg cgtaaaaaaa
      601 gcggtggccc tgcggcccat caccaaggcg gaggccatcc tcccctatct ggaggagctg
      661 gacctggtgc tggtgatgac ggtggagccc ggcttcggcg gccaggcctt catggagtcc
      721 cagctggaca ccatccgcca ggtgcgcgcc ctcatcgagc ggtacaaccc cgcctgcgaa
      781 ctggaggtgg acgggggcat ctcccccaag accgcccccc tggtggtgga ggcgggggcc
      841 aacgtgctgg tggccggctc cgctgtctac ggggcggagg acatccccgc cgccattcag
      901 gccctccgcg tgtcaggggg ggtgcgcggt gagagagtat aagatcctct ccgctgacag
      961 cctggagcag gcggagcagg tcatgaacga catggccctg gccgggtggc ggaccacctc
     1021 cctcacgccc tatcccgccg gctttccggc cggtgtcagc tatctgtttg ccgtcgtgtt
     1081 ggagcgggag cgctgatctc cccctgttca caccgtgtac tatttacgac tccggaggtc
     1141 tttcccatgc agtatttccc ccccgcactg gaaaatttgg tggagcagtt cgcccgcctg
     1201 cccggcgtcg gcgtcaaatc cgcccagcgg ctggcgttcc acgtcctctc cctcccagag
     1261 gaggacgccg ccgcctttgc ccaggccatt ctgtccgcca agtccgccat ccactgctgt
     1321 ccggtgtgtc agaatctgac ggagggggac ggcccctgct ccatctgcgc cagccccaag
     1381 cgggacgcct ccgtcatctg tgtggtggcc gacccgaagg acgtggtggc catggagcgg
     1441 gcccgggagt accaggggct ctaccacgtg ctccacgggg tgatctcccc catgaaccac
     1501 gtggggccgg atgacctgca catcaagtcc ctggtagagc gggtggccgc cggcggggtc
     1561 caggaggtca tcatggccac caatcccgac acggaggggg aggccaccgc catgtacctt
     1621 tcccggctgc tgcgcccctt tggggtgaag atcacccgtc tggcctacgg catcccggtg
     1681 ggcagccacc tggagtacgc cgacgacgcc accctgatgc gggccctgga gggccgtcag
     1741 gagatgtgag aatttgaacg ccgcactccc gtgggagtgc ggcgttttta cgcctgtttt
     1801 tctgggcatt ttcgacggcg gcgcatgggc accatttctt cgggacgctg actggatcgg
     1861 cttgatctcc ccccagattt ttaaaactta tcgcaatata ccaattttaa attactacat
     1921 gttttgactc ggcatctgat atggtcactt ttcaaggcgc ctcctgacgc cgccgctgcg
     1981 cgcggcagag cgggcccggc ccgcttctcc tcccttctgc gggaaaagat gcgctttacg
     2041 gggcttggcc ccggtgctat aataggctca aatcagacaa aggacaggag gccatcttca
     2101 tggtgaatca acgcatgttg gggctgggca ccgcccggtc cgtcatccgc gagctctttg
     2161 aatacggcaa gatacgtgcc gcccaggtgg gggcggagaa tgtcttcgat ttctccctgg
     2221 gcaaccccag cgtcccctgc ccccaggagg tcaacgacac cgccgtccgc atccttcggg
     2281 agcagccgga ggtcatccac tgttacacca gcgcccaggg tgaccaggag gcccgccagc
     2341 gcttcgccga ctccctcaac cgccgcttcg gcacctgcta taccgccgac cagttctaca
     2401 ttaccgtggg ggccgccgcc tccctgtgct gtgtgttcaa cggcctgacc tgccctgggg
     2461 acgagttcgt cgtctttgcc ccctacttcc cggagtataa ggtctttatc gagggcgctg
     2521 gggccaagat ggtgctc
//
LOCUS       DBMDSJ010000260         2522 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9806_length_2522_cov_1.661710, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000260 DBMDSJ010000000
VERSION     DBMDSJ010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9806_length_2522_cov_1.661710"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="ACZPAQ_06745"
     CDS             complement(<1..286)
                     /locus_tag="ACZPAQ_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR0903720.1"
                     /translation="MSDQFSRSRMLLGDGPQERLFHASVALFGVGGVGGFCAEALARA
                     GIGALDLYDDDTVSESNLNRQLIALHSTLGRPKAEVMAERVRDINPACRVR"
     gene            626..1678
                     /locus_tag="ACZPAQ_06750"
     CDS             626..1678
                     /locus_tag="ACZPAQ_06750"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR0903721.1"
                     /translation="MKIVVLAGGLSPERNVSLSSGAMVSEALREQGHQVALVDLFFGT
                     EGVEGPAEALYDAPVPQAFKRIGRQAPDLAQVRAARRDRSPSSIGPGVLELCAGADVV
                     YLALHGACGEDGRIQAALDLLGVPYTGSGCLASAIAMDKDLTKRLVAGQVTTPAWRSV
                     TVTEENLEELARQTRLPVVVKPVDSGSSIGVFIARDGQELRRALEESRKLGGRTVLEE
                     YIQGREIQVAVLEDRALPSIEIIPKEGFYDYENKYQPGAALEVCPAQIPPEWEQRLGE
                     AALAVFRTIGLSVYARADFIVTPDGTPYFLEINTLPGMTPTSLVPQEAAAVGIGYGAL
                     CQTIVEASLKARKGGA"
     gene            1683..>2522
                     /locus_tag="ACZPAQ_06755"
     CDS             1683..>2522
                     /locus_tag="ACZPAQ_06755"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR0903722.1"
                     /translation="MEAITLAQLLEAVHGTLLGDFRDENVIVQRVDTDSRTIHSGSLF
                     IPLVGERFDGHAYINAALEGGALGCLTARERESYRPDRFYIKVASTQRALRDLAAWYK
                     ARFPIPFIAVTGSVGKTTTKDMIAAVLSTRYRVLKTEGNYNNNIGLPLTLLRLDRSCE
                     ICVLEMGMDRPGEIDYLGEIVQPDVGVITNIGDAHIERLGSRENIFRAKCELLPHIRP
                     GGLVVLNGDDPMLASLRGRTPVPAVFCGREEGLEYRARRLEGDGDSRILCQMTTPRMD
                     RQVE"
BASE COUNT          440 a          807 c          892 g          383 t
ORIGIN      
        1 cccgcacccg gcaggcgggg ttgatgtccc ggacccgctc cgccatcacc tccgccttgg
       61 ggcggcccag ggtggagtgg agggcgatga gctgccggtt caggttgctc tcggagacgg
      121 tgtcgtcgtc gtacaggtcc agggcgccga tgccggcccg ggccagggcc tcagcgcaga
      181 agccccccac gccgcccacc ccgaagaggg ccacagaggc gtggaaaagc cgctcctggg
      241 ggccatcgcc caggagcatg cgggaacggg agaattgatc ggacacaggt cccacctcgt
      301 ctttccaaag gggtgccccg cccgccgggc ggggcgttct ttcttggcgc gtgcggtgac
      361 ggctgaggga atttcccggg gctctgcgga aaagatgcag atccggcctc tgtttttgtc
      421 accactcggg ccatcatatc ataagatttg ggtgagttca acggcaagga gggcgggaca
      481 gagcgcaaaa gcgggggaaa agggataaaa ttcccgtgga aggaggcaaa atgagaaaag
      541 gccattgagt attccaggga tatgtgctaa aatatttggc tggcatatta ttatttaaga
      601 cagacctgat gaggtgttga ataggatgaa gatcgtggta ttggcgggcg ggctcagccc
      661 ggagcgcaac gtctccctgt cctccggcgc tatggtgagc gaggccctgc gggagcaggg
      721 ccatcaggtg gcgctggtgg acctgttttt cgggacggag ggggtggagg gcccggcaga
      781 ggccctctac gacgccccgg tgccccaagc gttcaagcgg atcggccggc aggcccccga
      841 cctggcccag gtgcgcgccg cccgccggga caggagcccc tcgtccatcg gccccggggt
      901 gctggagctg tgcgccggcg cggacgtggt ctatctggcc cttcacggcg cctgcggcga
      961 ggacggccgc atccaggcgg ccctggatct gctgggagtg ccctacaccg gctccggctg
     1021 cctggccagc gccatcgcca tggataagga cctgaccaag cgcctggtgg cgggtcaggt
     1081 caccaccccg gcctggcgct ccgtcaccgt gacggaggag aacctggagg agcttgcccg
     1141 gcagacccgc ctgcctgtgg tggtcaagcc ggtggacagc ggctcctcca tcggggtctt
     1201 catcgcccgg gacggacagg agctgcgccg ggccctggaa gagagccgga agctgggggg
     1261 ccgcaccgtg ctggaggagt atatccaggg cagggagatc caggtggcgg tgctggagga
     1321 ccgggccctg ccctccatcg agatcatccc caaggagggc ttctacgact acgagaacaa
     1381 gtaccagccc ggcgcggccc tggaggtctg cccggcccag atccccccgg agtgggagca
     1441 gcggctgggg gaggcggcgc tggccgtctt tcggaccatc gggctgtccg tgtacgcccg
     1501 ggcggacttt atcgtgacgc cggacgggac cccctatttc ctggagatca acaccctgcc
     1561 cggcatgacc cccaccagtc tggtgcccca ggaggcggcc gcggtgggca tcggctacgg
     1621 cgccctgtgc cagaccatcg tggaggcgtc tctgaaagcc agaaaaggag gagcctgacc
     1681 agatggaagc catcactctg gcccagcttc tggaggccgt ccacggcacg ctgctgggcg
     1741 acttccggga tgagaacgtg atcgtccagc gggtggacac cgacagccgg accatccact
     1801 ccggctccct gttcatcccc ctggtgggag agcggttcga cgggcacgcc tacatcaacg
     1861 ccgccctgga ggggggcgct ctgggctgcc tcaccgcccg ggaacgggag agctaccgcc
     1921 ccgacagatt ctatatcaag gtggccagca cccagcgggc cctgcgggac ctggccgcat
     1981 ggtacaaggc ccgattcccc atccccttca tcgccgtgac gggcagcgtg ggcaagacca
     2041 ccaccaagga catgatcgcc gccgtgctga gcacccggta ccgggtgctc aagaccgagg
     2101 gcaattacaa caacaacatc ggcctgcccc tgaccctgct gcggctggac aggagctgtg
     2161 agatctgcgt gctggagatg gggatggacc gcccggggga gatcgactac ctgggggaga
     2221 tcgtccagcc ggacgtggga gtcatcacca acatcgggga cgcccacatc gagcgcctgg
     2281 gcagccggga gaacatcttc cgggccaagt gcgagctgct gccccacatc cggcccggcg
     2341 ggctggtggt gctcaacggg gatgatccca tgctggcatc cctccggggc cgcaccccgg
     2401 tgcccgccgt cttctgcggc cgggaggagg gcctggagta ccgggcccgc cggctggagg
     2461 gggacgggga cagccgcatc ctctgccaga tgaccacccc ccggatggac cggcaggtgg
     2521 ag
//
LOCUS       DBMDSJ010000261         2518 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9821_length_2518_cov_1.712867, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000261 DBMDSJ010000000
VERSION     DBMDSJ010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9821_length_2518_cov_1.712867"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="ACZPAQ_06760"
     CDS             <1..689
                     /locus_tag="ACZPAQ_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HflX GTPase family protein"
                     /protein_id="HIR0903723.1"
                     /translation="NEVPVVAIVGYTNAGKSTLLNRLTGAAIPANDRLFDTLDTTTRT
                     LEISDTCTVLISDTVGFIRKLPHHLVEAFKATLEELEYADLLLHVIDASNPEWRDQTA
                     VVEDLIRELGAEQTPRLDVFNKCDRYLGDIRPHGEDIVSISARTGEGVPELLAAIGRR
                     LDNGARRVIIHLPYDQGGLLDKLYQQAKVESVEYGETIDVAAVCTPKVIGQLGPLVEG
                     WRPRREPWED"
     gene            732..1238
                     /locus_tag="ACZPAQ_06765"
     CDS             732..1238
                     /locus_tag="ACZPAQ_06765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903724.1"
                     /translation="MLRPWRPEDAPSIARHADDPQVAANLRDVFPCPYQLSDAEEFIR
                     LCRAAEPEQAIFRAIVVDGQAVGSVALTRGTDVCRRSAELGYWLGRALWGRGIMTKAV
                     EEMCRTGFEAWDIVRIYAEPFAHNAGSRRVLEKAGFALEGVMRCGIWKNGRLRDYCMY
                     ALLRPETP"
     gene            complement(1199..1450)
                     /locus_tag="ACZPAQ_06770"
     CDS             complement(1199..1450)
                     /locus_tag="ACZPAQ_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903725.1"
                     /translation="MEHELDLLDHLNDTVTRITEDTLLLSLLLDYMDGLSCRPEQVSR
                     EISENLLQRLGDSLEQDAGALRRLWQSYGVSGRSRAYMQ"
     gene            1580..1774
                     /locus_tag="ACZPAQ_06775"
     CDS             1580..1774
                     /locus_tag="ACZPAQ_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903726.1"
                     /translation="MEDYRAILKRNREKQGLSQNKLAKRLGVTQTFISEIERGRKNPS
                     LEQFFRICKELQIKVFPDEP"
     gene            2213..>2518
                     /locus_tag="ACZPAQ_06780"
     CDS             2213..>2518
                     /locus_tag="ACZPAQ_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HIR0903727.1"
                     /translation="MTEYYIPTQAGEAELVEKRSRFIGHVWRVESEEEARARIEEMKK
                     RYYDARHNCWCYLLREGPVRYSDDGEPQGTAGQPMLNVFQREEVTNVCCVVTRYFGGI
                     "
BASE COUNT          486 a          764 c          840 g          428 t
ORIGIN      
        1 agaacgaggt accggtggtg gccattgtgg gctataccaa tgcgggcaaa tccaccctgc
       61 tcaaccggct caccggggcg gccatcccgg ccaatgaccg actgttcgac accctggaca
      121 ccaccacccg caccctggag atctccgaca cctgcacggt gctcatctcc gacacggtgg
      181 gctttatccg caagctgccc caccatctgg tggaggcctt caaggccacc ctggaggagc
      241 tggagtacgc cgacctgctg ctccatgtga tcgatgcctc caacccggag tggcgggacc
      301 agacggcggt ggtggaggac ctgatccggg agctgggggc cgaacagacc ccccgcctgg
      361 atgttttcaa caaatgcgac cggtatctgg gggacatccg gccccacggg gaggacatcg
      421 tctccatctc cgccaggacc ggcgaggggg tgcccgaact gctggccgcc atcggcaggc
      481 ggctggacaa cggcgcccgc cgggtgatca tccatctgcc ctacgaccag ggcgggctgc
      541 tggacaagct ctaccagcag gccaaggtgg agtcggtgga gtacggcgag accatcgatg
      601 tggcggcggt ctgcaccccc aaggtgatcg gtcagctggg cccgctggtg gagggctgga
      661 ggccacgtcg ggagccctgg gaagactgaa agccccatct cagaaaagaa agggaaagga
      721 ccatggacgt gatgctcaga ccctggcgcc cggaggacgc cccctctatc gcccggcatg
      781 ccgatgaccc ccaggtggcc gccaacctgc gggatgtgtt cccctgcccc taccaactgt
      841 cagacgcgga ggagttcatc cgcctgtgca gggcggcgga gccggagcag gccatattcc
      901 gggccatcgt ggtggatgga caggccgtgg gcagcgtggc cctcacccgg ggcacggatg
      961 tctgccgccg gtcggccgag ctgggctact ggctgggccg ggccctctgg ggccggggca
     1021 tcatgaccaa ggcggtggag gagatgtgcc ggacgggctt tgaggcgtgg gacatcgtgc
     1081 gcatctacgc ggagcccttc gcccacaacg ccggctcccg ccgagtgctg gagaaggcgg
     1141 gcttcgctct ggagggcgtc atgcgctgcg gcatatggaa aaacggccgg ctgcgggact
     1201 actgcatgta cgccctgctg cggccggaga ccccgtagga ctgccacagg cggcggaggg
     1261 cgccggcgtc ctgctccagg ctgtccccca gccgctggag gaggttttcg gagatttccc
     1321 gggaaacctg ctccgggcgg caggagagac cgtccatata gtccagcaga agggagagca
     1381 gcagcgtgtc ctctgtgatg cgggtgaccg tgtcgttcag atggtccagc agatcgagct
     1441 cgtgttccat agatgattac ctccttctga tatagagctt aaagctatta tagcgaacag
     1501 ctcttgctcg gtcaagagct aaaagctata attttcaaaa caatagctat gagctataat
     1561 agagaagaag gtgagaacga tggaagatta tcgggcaatt ttgaaaagga accgagagaa
     1621 gcagggatta agtcaaaaca agctggcaaa acggctgggc gtgacacaga catttatcag
     1681 tgaaatagag cggggaagaa aaaatccatc gttggaacag tttttccgga tctgcaagga
     1741 attgcagatc aaagtctttc ccgacgagcc ctaaggggcg ccgcggcccc ggtccctgcg
     1801 cagggacgca cactgtgcgc ccgccgtccg tgaggatgcg gcccgatgcg ggcatcgggc
     1861 cctgctgggt gcgtgcttct ccctgtcttt gtgtaggggg tggggcttgc ccccgcccgc
     1921 cggtcccagg tctgcgatgt gccgcaggag tgatttccag acctgcccgg gctattgaat
     1981 cgctctggca gaagaacgcc ccctcatccg tcacggcttc gccgtgccac cttccccccc
     2041 ggaggagggg aaggcttttc gggcggctgg aggccgcccc tacatgatat tttcggcctt
     2101 tgcgcagggg cgcgcaatgc gcgcccgccg gtttcccacc agccgcccct attgtgccaa
     2161 tctaggcgcg ccccgcgagt attccccaaa acgtgcaaag gcaggtgacc ccatgacaga
     2221 gtattacatc cccacccagg ccggtgaggc ggagctggtc gagaagcgct cccgcttcat
     2281 cggccatgtc tggcgtgtgg agagcgagga ggaggcccgt gcccgcatcg aggagatgaa
     2341 aaagcggtac tacgacgccc ggcacaactg ctggtgctac ctgctgcggg aggggccggt
     2401 gcgctactcg gacgacgggg agccccaggg gacggcgggc cagcccatgc tcaacgtgtt
     2461 ccagcgggag gaggtcacca acgtctgctg tgtggtcacc cggtattttg ggggcatc
//
LOCUS       DBMDSJ010000262         2510 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9854_length_2510_cov_1.706102, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000262 DBMDSJ010000000
VERSION     DBMDSJ010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9854_length_2510_cov_1.706102"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="ACZPAQ_06785"
     CDS             complement(<1..379)
                     /locus_tag="ACZPAQ_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903728.1"
                     /translation="MDRTSQAEETVRRYSDMVYRLAFARTGNTADAEDVYQEVFLRYL
                     RSDPQFTSEEHRKAWLLRVTINCAKKLHAAPWRRRTEPLSETLEAPSPDGEALWEELR
                     RLPDKYRTVLHLYYYEDMTTEEIA"
     gene            764..1471
                     /locus_tag="ACZPAQ_06790"
     CDS             764..1471
                     /locus_tag="ACZPAQ_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0903729.1"
                     /translation="MDTKETVLVVDDDREIVGAIALALEKEGYRVLRAYDGMEALDHA
                     LDPELRLILMDVMMPRLDGLSAVLRIRERRNLPIIVLSAKSEESDKVLGLSMGADDYV
                     TKPFSTQELVARVRSQLRRYTRLGDVNASARDRRIVSGRLSYDPDSRVLTADGDEVKL
                     TATELGILDLLMCNLGRVFPAEEIYRRVWGEEAYASENTVMVHIRRIREKIELNPREP
                     DYLKVVWGIGYKMEKHP"
     gene            1443..>1699
                     /locus_tag="ACZPAQ_06795"
     CDS             1443..>1699
                     /locus_tag="ACZPAQ_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903730.1"
                     /translation="MVIKWKNILNRGNKTDRAPAPAACPAGGRLRAAAGLTAFLLGIS
                     LLLAGGLGQYGWWSTELGARWARDSLEEDWQDTAAFRTIIC"
     assembly_gap    1700..1709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1710..>2510
                     /locus_tag="ACZPAQ_06800"
     CDS             <1710..>2510
                     /locus_tag="ACZPAQ_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903731.1"
                     /translation="NWLDACLTAGTWEYTSGGDYGKELLDGLEGDKNLLYAVARRGSV
                     LYTNTSDGGFLSSGQPPEGYRFALRCEDGMVSITQDGRELDVYGDGWYTEDDLWFVPG
                     YENFPMDADARNIQVYLAAASDPKPMVYGNYLGSGADICTSQLYDLVQNLAQYRRELL
                     TEWGMLGGGALLLALALVLRRDKARADRFLARGTGRIWFEVKVLLALLALLLLPWGEL
                     SSWSSETPVASYTAASSTASSGSAQWDTGAVALYSYSGDVVQSQAETHV"
BASE COUNT          444 a          765 c          846 g          445 t
ORIGIN      
        1 gggcgatctc ctccgtggtc atgtcctcgt agtagtagag gtggagcacc gtgcggtact
       61 tgtccggcag gcggcgcagc tcctcccaga gcgcctctcc atccggggag ggggcctcca
      121 gcgtctcact gaggggctcc gtccgccgcc gccagggcgc cgcatgaagc tttttggcgc
      181 agttgatagt gacccgcagc agccaggcct tccggtgctc ctccgaggtg aactgggggt
      241 cgctgcggag gtagcggaga aagacctcct ggtacacatc ctcagcgtct gcggtgttcc
      301 ccgtccgggc aaaggcaagg cgatagacca tgtcagagta gcggcggacc gtctcctccg
      361 cctggctcgt ccggtccatg tcatccccct cctttcacct caaacacccc acagcggggc
      421 aaaatgttgc agggatcaaa aaatttttgc gcggcccctg aaccggacct gcgcaggggc
      481 acacagtggg tgtctgcctc atagaagcgg gacgggagaa gaaagacccc tcatccgtca
      541 cggcttcacc gtgccacctc cccccagagg ggggaaggct ttgggggcgg tccgcacctt
      601 tcctcttttg tgatctcctc atttatggta tatatatatc aggaaaaatg gaagggccgg
      661 cggccgggcc ccatctgtaa gaaacttgta agacttcccc cagctttttt gtaagaaatc
      721 tctgctatcc tgttcccagg caaaccgaga gaggagctgc gctatggaca caaaggagac
      781 cgtcctggtg gtggatgacg accgggagat cgtgggggcc atcgccctgg ccctggaaaa
      841 ggagggctac cgggtgctgc gtgcctatga cggcatggag gccctggacc acgccctgga
      901 cccggagctg cggctgatcc tgatggatgt gatgatgccc cgcctggacg gcctctccgc
      961 cgtcctccgc atccgggaac ggcgcaacct gcccatcatt gtcctgtccg ccaagagcga
     1021 ggagagcgac aaggtgctgg gactgtccat gggggcggat gactatgtga ccaagccctt
     1081 ctccacccag gagctggtgg cccgggtgcg cagccagctg cggcggtaca cccggcttgg
     1141 ggatgtgaac gcctccgcca gagaccggcg catcgtcagc ggccggctgt cctacgaccc
     1201 ggacagccgg gtcctgacgg cggacgggga cgaggtgaag ctcaccgcaa cggagctggg
     1261 catcctggac ctgctgatgt gcaacttggg ccgggtcttt cctgcggagg agatctaccg
     1321 ccgggtctgg ggggaggagg cctacgcctc ggagaacaca gtgatggtgc acatccgccg
     1381 gatcagggag aagatcgagc tcaaccctcg ggagccggac tatttgaagg tggtgtgggg
     1441 aattggttat aaaatggaaa aacatcctta acagaggcaa caagacagac agggccccgg
     1501 caccggcggc gtgcccggcg gggggccgcc tgcgggcggc ggcggggctg acggctttcc
     1561 tgctggggat cagcctcctg ctggccggcg gattgggcca gtacggctgg tggtccacgg
     1621 agctgggcgc ccggtgggcc agggacagcc tggaggagga ctggcaggac accgccgcct
     1681 tccgaactat catctgcaan nnnnnnnnnc aactggctgg acgcctgtct caccgcgggg
     1741 acctgggagt acacctccgg aggcgactac gggaaggagc tcctggatgg cctggagggg
     1801 gacaagaacc tgctctacgc cgtggcgcgg cggggcagtg tcctgtacac caacacctcg
     1861 gacggcggct tcctctcctc cggccagccg ccggaggggt accgcttcgc gctgcgctgt
     1921 gaggacggaa tggtgtccat tacccaggac ggccgggaac tggatgtata tggggacggc
     1981 tggtacaccg aggacgacct gtggttcgtc ccaggctatg agaacttccc catggatgcg
     2041 gatgccagaa acatccaggt ctatctggcc gcggcgtcag atccaaagcc tatggtctac
     2101 gggaattatc tgggctccgg cgcggatatc tgcacctccc agctctatga cctggtccag
     2161 aacctggcgc agtaccgccg ggagctgctg acagagtggg ggatgctggg gggcggtgcg
     2221 ctgctgctgg ccctggctct ggtcctgcgc cgggacaagg ccagggcgga ccgcttcctg
     2281 gcccggggga ccggccggat ctggttcgag gtcaaggtgc tcctggccct tctggcgctg
     2341 ctcctcctcc cctgggggga gttgagcagt tggtcctccg aaacacccgt cgcgtcctat
     2401 acagcggcct cttccaccgc cagcagcggc tccgcacagt gggacacggg ggccgtcgcc
     2461 ctctattctt acagcgggga tgtggttcag tcccaggcag agacccatgt
//
LOCUS       DBMDSJ010000263         2491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9938_length_2491_cov_1.874895, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000263 DBMDSJ010000000
VERSION     DBMDSJ010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9938_length_2491_cov_1.874895"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="ACZPAQ_06805"
     CDS             complement(<1..982)
                     /locus_tag="ACZPAQ_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR0903732.1"
                     /translation="MNLSRKWLSEFVSVEADDKEFAESMTLSGSKVELTHDLGAEIQN
                     VVVGKVLSMEHHPDSDHMWVCQIDVGREEPVQIVTGAWNVHIDDLVPVAMHKSTLPGG
                     KKIEKGKLRGVVSNGMLCGLSELGLDTRDFPYAVITPAAILGDYKPLDKDKPSISADI
                     QPGDKIYGPVIAALVEGVEPAGEAGWTCRLFWAGQEALSVTVTTTCANLHNGDLVAYD
                     TKRGAVCTLDDLHAQQAEFPHCIPDGIFILHEDCKPGDDLKPVVGLDDHVVEFEITPN
                     RPDCLSIIGLAREASATFDAPLALHQPVVKGGGPGVLPELLDVETPAADLC"
     gene            complement(1138..2163)
                     /gene="pheS"
                     /locus_tag="ACZPAQ_06810"
     CDS             complement(1138..2163)
                     /gene="pheS"
                     /locus_tag="ACZPAQ_06810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR0903733.1"
                     /translation="MKEQLAKIRSEALAAFGQAQSAAELDSLRVQYLGKKGELTAVLK
                     MMGKLSAEERPAMGQMANEVRSALEAAIEAASGKLEAQALEQRLKDEAVDVTIPGREV
                     KLGHKHPMYLALDEIKDIFIGMGFTVLDGPEVELAEYNFDKLNAEEGHPSRDWSDTFY
                     FDEDSRVMLRSQTSPMQVRAMETMELPIRIIAPGRVYRKDEVDATHSPMFHQVEGMVI
                     DKGVTMADLKGTLNTVVEQLYGKGTKTRFRPHHFPFTEPSCEMDVQCHKCGGVGCPTC
                     KGEGWIELLGAGMVHPKVLKMAGIDPEVYSGWAFGMGLERTAMRRFKIADLRLIFEND
                     VRFLEQF"
BASE COUNT          462 a          781 c          742 g          506 t
ORIGIN      
        1 ggcacaggtc ggcggcgggg gtctccacat ccagcagctc cggcaacacg ccggggccgc
       61 cgcccttcac caccggctgg tggagggcca ggggtgcgtc aaaggtggcg gaggcctccc
      121 gggccaggcc gatgatggag aggcagtcgg ggcggttggg ggtgatctcg aactccacca
      181 cgtggtcgtc caggcccacc accggcttca ggtcgtcccc gggtttacag tcctcgtgaa
      241 ggatgaagat gccgtcgggg atgcagtggg ggaactccgc ctgctgggcg tggaggtcat
      301 ccagggtgca gacggcccct cgcttggtgt cataggccac caggtcgccg ttgtggagat
      361 tggcacaggt ggtggtgact gtcacggaca gggcctcctg gccggcccag aacaggcggc
      421 aggtccagcc ggcctctccg gcgggctcta ctccctccac cagggcagct atcaccggcc
      481 cataaatctt gtcccccggc tggatgtccg cggagataga gggcttgtcc ttatccaggg
      541 gcttgtagtc ccccaggatg gcggcaggag tgatgacggc gtaggggaag tcccgggtgt
      601 ccaggcccag ctcactcagg ccgcacagca tgccgttgga caccacgccc cgcagcttgc
      661 ccttttcgat cttcttgccc ccaggcaggg tggacttgtg catggccaca ggcaccaggt
      721 cgtctatgtg gacgttccag gccccggtga cgatctgcac cggctcctcc cggcccacgt
      781 cgatctggca cacccacatg tggtcgctgt ccgggtggtg ctccatggac agcaccttgc
      841 ccaccaccac gttctggatc tcggcaccca ggtcgtgggt cagctccacc ttggagccgg
      901 acagggtcat ggactccgca aactctttat catcggcctc aacgctcaca aactcgctga
      961 gccacttgcg agatagattc atagtataaa actccctttg tttgaattag gaatgaggag
     1021 ttaggaatta ggaattgtgg tgtccggttt tgtcggaata tttggaattg agctgcggaa
     1081 gaaaccgctt caatttctaa ctcctaattt ctaattccta attgattttt agagcaatca
     1141 gaattgctct aaaaatctaa cgtcgttctc gaaaatcaga cgcaggtcag cgatcttgaa
     1201 gcggcgcatg gcggtgcgct ccagccccat gccgaaggcc cagccggagt agacctccgg
     1261 gtcgatgccc gccattttca gcaccttggg gtggaccatt ccggcaccca gcagctcgat
     1321 ccagccctcc cccttgcagg tgggacagcc cacgccgccg cacttgtgac actgcacgtc
     1381 catctcgcag gaaggctcgg tgaaggggaa atggtgagga cggaagcggg tcttggtgcc
     1441 cttgccgtaa agctgctcca ccaccgtgtt cagagtgccc ttcagatcgg ccatggtgac
     1501 ccccttgtcg atgaccatgc cctccacctg gtggaacatg ggggagtggg tcgcatccac
     1561 ctcatcctta cggtacaccc ggccgggggc gatgatgcgg ataggcagtt ccatagtctc
     1621 catggcccgc acctgcatgg ggctggtctg ggagcggagc atcacccggc tgtcctcgtc
     1681 aaagtagaag gtgtccgacc agtcacggga ggggtgtccc tcctccgcat tgagcttgtc
     1741 gaagttgtac tcggccagct ccacctccgg gccgtccagc acggtgaagc ccatgccgat
     1801 gaagatgtcc ttgatctcat ccagggccag gtacatgggg tgcttgtgtc ccagcttcac
     1861 ctcgcggccg gggatggtca catccaccgc ctcgtccttc aggcgttgct ccagggcctg
     1921 ggcctccagc ttaccggagg cggcctcaat ggccgcctcc agagcggagc ggacctcgtt
     1981 ggccatctgg cccatggcgg ggcgctcctc ggcggacagc ttgcccatca ttttcagaac
     2041 ggcggtgagc tcacccttct tgcccagata ctggacgcgc aggctgtcca actcggcggc
     2101 ggactgggcc tgcccaaagg cggccagcgc ctccgaacgg atctttgcta actgttcttt
     2161 catctgtgtt cttcctttca gaggtaaatt gatcggacgg gggcggacag caaaaaagcc
     2221 ttccgcccca aacctgttgg gacgaaaggc aacccttccg cggtaccacc cgtattcgtg
     2281 tcagacacgc acttgaactt cccgataacg gcggaacagc cgtccccctc tcaggggccg
     2341 ctcccgggcg aaccaaacga cacgcttcag ggcggctttc agccggtgac cacccctctc
     2401 tgagaaacga aagcacgtta ttttcccgct cttcgcattt gtactaaagg tatatatacc
     2461 acaaatcttc ttgtattgca agtccccgga g
//
LOCUS       DBMDSJ010000264         2482 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_9986_length_2482_cov_1.532549, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000264 DBMDSJ010000000
VERSION     DBMDSJ010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9986_length_2482_cov_1.532549"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /gene="fusA"
                     /locus_tag="ACZPAQ_06815"
     CDS             <1..658
                     /gene="fusA"
                     /locus_tag="ACZPAQ_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494182.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR0903734.1"
                     /translation="VAYREKIRKTVSKQGRHKKQTGGAGQFGDVWVRFEPNPESDQMV
                     FAEEVFGGSVPKNYFPAVEKGLREACVHGPLAGYPVVNLKAVLYDGSYHPVDSSEIAF
                     KTAAQLAYKAAMPEANPVLMEPIGELKVTIPDSYMGDVIGDLNKRRGRIMGMNPTKDG
                     EQVVEAEVPMAEMSTYAIDLRAMTQSRGSFTFHFVRYEDCPPAAQQKAIEEAKALAEE
                     "
     gene            complement(1211..2278)
                     /locus_tag="ACZPAQ_06820"
     CDS             complement(1211..2278)
                     /locus_tag="ACZPAQ_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903735.1"
                     /translation="MKFHEILRQRRLALGLTQEQLAQRLGVSAPAVNKWERNNSYPDI
                     TMLPPLARLLEVDLNTLLSFQEELTEEEIGAFANVLGERAQQEGCEAAFRLARDKLRE
                     YPNSDLLAYTAAQILDGALVLWKRGENDPEREQAWKGEILALYRRCADSGDRRVRERA
                     ERMLISQYMAQGELEQAEEQLARLPQEDRERPMLEAMLRRKQKRSEEAWPILERELFR
                     QASDLQSTLMALMDLALEAGDKTCARQYSETAEQAGRVFQLTAYAVASAPLQLALAEQ
                     DGPEALAVLERLLRSLEEPWDLSASPLYRHLPTKEAAREVQQIMLQYLLEELERDPES
                     AFLRETPGYQEMLERVHRAEI"
BASE COUNT          426 a          851 c          700 g          505 t
ORIGIN      
        1 ggtggcctat cgtgagaaga tccgcaagac agtctccaaa cagggccgcc acaaaaagca
       61 gaccggcggt gccggccagt tcggcgatgt ctgggtccgc tttgagccca atccggagag
      121 cgaccagatg gtctttgccg aggaggtttt cggcggctcc gtgcccaaga actatttccc
      181 cgctgtggag aagggcctgc gtgaagcctg cgttcacgga cctctggcgg gctaccccgt
      241 ggtcaacctg aaggctgtcc tctatgatgg aagctaccac cccgtggact cctccgagat
      301 cgctttcaag accgcggccc agctggccta caaggccgcc atgcctgagg ccaaccccgt
      361 gctcatggag cccatcggcg agctgaaggt caccattcca gacagctaca tgggcgacgt
      421 catcggtgac ctgaacaagc gccgcggccg catcatgggc atgaatccca ctaaggacgg
      481 cgagcaggtg gtggaggccg aggtccccat ggccgagatg tctacctacg ccatcgacct
      541 gcgggccatg acccagtccc gcggctcctt caccttccac tttgtccgct atgaggactg
      601 ccctcccgcg gcccagcaga aggccattga ggaagccaag gccctggcgg aagaataaat
      661 catcccgggc acccgttccc cgcagggagc gggtgccttt tgcgggcagt ccgagtcagg
      721 cgggctgcgc ccgcccggcg cttttgcgcc gcaaattcgc agcgtagcgg agaattttca
      781 cggggcggat ccactccgcc ccgtgtcttc tctccaaggg tccccgccat ggaactccat
      841 ggcgggctgg actgccctcc cgcggcccag cagaaggcca ttgaggaagc caaggccctg
      901 gcggaagaat aaatcatccc aggcacccgt ttccccacag ggagcgggtg ccttttgcgg
      961 gcagttcccc ggtccggccc catacagcgc gccagcccgg tggtccctgg accaccgggc
     1021 tggctctgtg ctctgttctg tcgcagacgg acacggctcc accacgcttc ggccgccccg
     1081 ggggaagcag gggggaggta tccctgtccc gcccggcgcg ctgccgtgaa cctgtctcat
     1141 cttgtcccca tcatactcct ccccaatcca tttttcaaat cgatctttct tataaaataa
     1201 ttgattttat ctatatttct gcccggtgaa cccgctccag catctcctgg tatccggggg
     1261 tctcccggag gaaagcggat tccgggtccc gctccagttc ctccagcaga tattggagca
     1321 tgatctgctg gacttccctc gcagcctcct ttgtgggcaa gtggcggtag agaggggacg
     1381 cactcaggtc ccagggctcc tccaggctgc gcaggagccg ttccagcacc gccagcgcct
     1441 cggggccgtc ctgctccgcc agcgccagct ggagcggggc agacgccact gcgtaggcgg
     1501 tgagctggaa cacccgtccc gcctgctccg ccgtctcact gtattgccgc gcgcacgtct
     1561 tgtcccccgc ctccagcgcc aggtccatca aagccatgag ggtagactgg agatctgatg
     1621 cctgccggaa cagctcccgc tccagaatgg gccacgcctc ttccgaccgc ttctgtttcc
     1681 tgcggagcat ggcctccagc atgggccgtt cccggtcctc ctggggcaga cgggccagct
     1741 gttcctctgc ctgctccagc tctccctggg ccatgtactg ggagatgagc atccgctccg
     1801 cccgctcccg cacccgcctg tctccactgt ccgcgcaccg gcggtagagg gccaggatct
     1861 cccccttcca cgcctgctcc cgctccgggt cattctctcc ccgcttccag agcaccagcg
     1921 ccccgtccag tatctgggcc gccgtatagg ccagcaggtc gctgtttggg tactcccgca
     1981 gcttgtcccg ggccagacgg aaggcggcct cacagccctc ctgctgggct ctctccccca
     2041 gcacattggc aaatgctccg atctcctcct ctgtcagttc ctcctgaaag gagagcaggg
     2101 tgttcaggtc cacctcaagc agacgggcca gcgggggcag cattgtgata tccggatagg
     2161 agttgttccg ctcccacttg ttgacagccg gcgctgacac ccccagccgc tgggccagct
     2221 gttcctgggt cagccccagc gccaggcgcc gctgccgcag gatctcgtga aatttcatgt
     2281 tccgtccccc ttttccttgg tgtgcccatt gtatcagagg aggccttttc tccgcaaccg
     2341 aagggtattc acctttcctt ttaaaaaatt aaccgctgtt catatttttt cctgttttta
     2401 aaaaaattgt cctctgactg tggtcagagg acaaaattgt gctgaaatat gcacaatttt
     2461 ccgcttttca ttcccttgac ag
//
LOCUS       DBMDSJ010000265         2460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10077_length_2460_cov_1.274693, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000265 DBMDSJ010000000
VERSION     DBMDSJ010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10077_length_2460_cov_1.274693"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            119..865
                     /locus_tag="ACZPAQ_06825"
     CDS             119..865
                     /locus_tag="ACZPAQ_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903736.1"
                     /translation="MSHPNPIPPMTEEERAAFRSFLLRRYVLSILALVLGIAGAVLLL
                     RRGVDGPPLMEAVWVCLFVLSGGTAVLGGRAWGRYQTLLTEPEAMERTWQARRAGPQT
                     EADQSYRRDVSRSGRLGLLLLAAALLLLLLGIPLQAQLTGRAADVYGQVIFCLFLVGF
                     SMMAGSWHTLARPEELRSARVARDDERSQEIELRAVWKAARATQTALFLGLLAAGWLG
                     WSDVSLTLSIVLFFYFSALAVFRARLSKRM"
     gene            876..1808
                     /locus_tag="ACZPAQ_06830"
     CDS             876..1808
                     /locus_tag="ACZPAQ_06830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR0903737.1"
                     /translation="MEQIQRKTRRLELVLGPEDGGVKVDTLLRRRLHLSGTVIRRIKW
                     LPDGILVDGARVNTAFRPREGQTLSVRLSDPERRSGILPAPGPLDIVYEDGDLLILNK
                     APGVPVHPGPGHFNDTIGNFLLNYYNSEGIEGDFHPVHRLDRGTSGLMAVAKHPHAQE
                     VLKGQLHTPAFRRVYLAVCDGGPQPPSGVVDAPLGPREGSLVEQMVRPDGKDARTRYE
                     TLSRSGGRSLVRLELDTGRTHQIRVHMASLGCPLTGDFLYGYEDRSLISRPALHSAQL
                     SLRQPVTGQELSFTLPLPEDMARLLTHQKGEKEP"
     gene            1805..2428
                     /locus_tag="ACZPAQ_06835"
     CDS             1805..2428
                     /locus_tag="ACZPAQ_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903738.1"
                     /translation="MNVIRRWITQPNALLRAAALFGAGMALTLAAACLSWPLLPEGLL
                     KNANPLWNWAVSSRPLVGGLQIFGFNLIFACVLVGANLFARGPQGGPMIPCGYSCLLL
                     LFLLYGLMVGTWSFQMGGEAPSFAARLLRMLHISEASGMVEMSAFCCIASGTADISFV
                     QTLGGRTTAGRLRDIRLSRQDGLVLALGFLLLFAAAMIEAASILRLT"
BASE COUNT          331 a          858 c          810 g          461 t
ORIGIN      
        1 cgatgctggt gaaggtgttg ctgggctcca tgctggccta catcctgttc ttccttggcg
       61 cccgggccgc cctggcccgg cggcggtgag aactttatcc cggaaaggag gaagccctgt
      121 gtcccatcca aacccaatcc cacccatgac ggaggaggag cgcgccgcct tccggtcctt
      181 cctgctccgc cgctacgtcc tcagcatcct ggccctggtc ctgggcatcg ccggggcggt
      241 gctgctcctc cgccggggtg ttgacgggcc gcccctgatg gaggccgtct gggtctgcct
      301 gtttgtcctc tccggcggga ccgccgtgct ggggggcagg gcctggggcc ggtatcagac
      361 tctgctcaca gagccggagg ccatggagcg gacctggcag gcccgcagag ccggccccca
      421 gacggaggcc gaccagtcct accgccgtga tgtgagccgg tcgggccgtc tgggcctcct
      481 gctcctggcc gccgccctgc tgctcctgct cctgggcatt ccccttcagg cccaactcac
      541 cgggagggcg gcggatgtgt atggccaggt gatcttctgc ctgtttctgg tcggcttcag
      601 catgatggcg gggagctggc acaccctcgc ccggccggag gagctgcgct ccgcccgggt
      661 ggcccgcgac gacgagcgca gccaggagat cgagctccgg gccgtgtgga aggctgcccg
      721 ggctacccag accgccctgt tcctcggtct cctggcggcc ggatggctcg gctggtcgga
      781 tgtctccctg accctgtcca tcgtgctgtt tttctacttt tccgctctgg ccgtcttccg
      841 cgcccggctg tccaagcgga tgtgagggtg gcgggatgga acagatacag aggaagaccc
      901 gccgcctgga gctggtcctg gggccggagg acgggggcgt caaggtggac accctgctgc
      961 ggcggaggct gcacctgtcc ggcacggtga tccgccggat caagtggctc cccgacggca
     1021 tcctggtgga cggggcccgg gtgaataccg ccttccgccc ccgggaggga cagactctgt
     1081 ccgtccgcct gtccgacccg gagcggcgca gcggcatcct gcccgccccg ggtcccctgg
     1141 atatcgtgta cgaggacggg gacctgctga tcctgaacaa ggccccgggg gtgccggtcc
     1201 accccggccc cggacatttt aatgatacga taggtaattt tttgctgaat tattacaatt
     1261 cggagggaat tgaaggagat ttccaccccg tccaccggct ggaccggggg acctccgggc
     1321 tgatggcagt ggccaagcac ccccacgccc aggaggtgct gaaggggcag ctccacaccc
     1381 ccgccttccg ccgtgtctat ctggcggtgt gcgacggcgg gcctcagccc ccctccggtg
     1441 tggtagacgc cccgctgggg ccccgggagg gctccctggt ggagcagatg gtccgccccg
     1501 acggcaagga cgcccgcaca cggtatgaga ccctgtcccg ctccgggggg cgttccctgg
     1561 tccgtctgga gctggacacc gggcgcaccc accagatccg ggtccacatg gcctctctgg
     1621 gctgcccctt gacaggagat ttcctttacg ggtacgagga ccgttccctg atctcccgcc
     1681 ctgccctcca ctccgcccag ctgtccctcc ggcagcccgt cactgggcag gagctgtcct
     1741 ttaccctccc ccttccggag gatatggcgc gcctgctgac ccatcagaaa ggagagaagg
     1801 agccgtgaat gtgatccggc gctggatcac ccagcccaac gccctgctcc gggcggcagc
     1861 tttgttcggg gcaggcatgg ccctgacgct ggcggcggcc tgcctcagct ggcctctgct
     1921 cccagagggg ctcctgaaaa atgccaatcc cctgtggaac tgggcggtct cctcccgccc
     1981 ccttgtgggc gggctccaga tcttcgggtt caacctgatc tttgcctgcg tcctcgtggg
     2041 ggccaacctc ttcgccaggg gaccgcaggg gggtcccatg atcccctgcg gctactcctg
     2101 tctcctcctg ctgttcctgc tctatgggct gatggtgggc acctggtcct tccaaatggg
     2161 cggagaagcg ccctctttcg cggcccggct gctgcgcatg ctccatatca gcgaggcctc
     2221 cggcatggtg gagatgtccg ccttctgctg catcgcctcg ggaacggcgg acatctcctt
     2281 cgtccagacc ctggggggcc ggaccaccgc cggacggctg cgggatatcc gcctgtcccg
     2341 tcaggatggg ctggtccttg ctttgggctt cctgctcctg ttcgccgccg ccatgatcga
     2401 ggcggccagc atcctccgcc tgacctgaac acagggcccg ggatcaaaaa tgattccggg
//
LOCUS       DBMDSJ010000266         2457 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10095_length_2457_cov_1.507216, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000266 DBMDSJ010000000
VERSION     DBMDSJ010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10095_length_2457_cov_1.507216"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="ACZPAQ_06840"
     CDS             <1..184
                     /locus_tag="ACZPAQ_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024934.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903739.1"
                     /translation="SASGQDQVSCTQEEIASSISVSRVTVSRILNQFSRRGLVALGYR
                     SISLLDRRTLETLCTP"
     gene            complement(224..>2457)
                     /locus_tag="ACZPAQ_06845"
     CDS             complement(224..>2457)
                     /locus_tag="ACZPAQ_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019843.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HIR0903740.1"
                     /translation="SSRGQRLLARCLEQGLGLPQDQKAALEWLRTSALQGDASSQAEL
                     ARHYEFGVGTEISPKQAVQWYRRAAQAGDADGQCCLGWLMEHGKWVEQDADGAVQLYS
                     QAAEQGHSRAALLLGICCLNGTGMPRDPDRAWDLLVRAWELGAESAACELGQMCLKGN
                     GVPQNDQEAVRWWTLGAQKGDSTAQYDLGWCCEKGRGIGQDYAQAAYWYRRSADGGDS
                     DAMCNLGWLYEAGQGVEQSWEKAAEWYLRSAEAGSGRAMGNLAWCYDNGKGVEQDHAE
                     AARWFQRGAESGDVRSMYCLAWDYEHGEGVEASLPLAAHWYEQASEAGDAEASRCLGW
                     LYETGQGVDQDGKKAAALYRRAMEAGSIRAMGNLAWCYSEGIGVEQDQVQATELYRKG
                     AEAGNPRSMSSFGYRLLHGRGIGKDDKQAVIWLRRAAEQEQDNAMCNLGWCYERGHGV
                     PQDWKQAVEWYRRAAQAGNTVAMGNLGWCYEKGKGVEKDPEAAVSWYRQGAERGSVRS
                     MYELGRCCQGGIGVAEDLKQALIWFHKSAEVGDADSLGLLGWSYEYGIGTEQDYARAV
                     EYYRCAAEKGNASSMNNLAEMLANGRGGPRNFEQAMFWYQRAAEAGSVCALYNMGWHA
                     EQAGRIAEALDCYRQAQQSGDEAAFWALGRFYEIGIGVEQDPKQAFALYQAGAEKGNV
                     NCRCRLIRCYALGIGTPRDPDMARQLGRALLCEKLDPWDLEDYGCQPELEQLRQLMDT
                     LET"
BASE COUNT          416 a          925 c          620 g          496 t
ORIGIN      
        1 ctcagcatct gggcaggatc aggtgtcctg cacccaggag gagatcgcct cgtccatctc
       61 tgttagccgg gtgaccgtca gccgcatcct gaaccaattc tcccgcaggg ggctggtcgc
      121 tctgggatac cggagcattt ctcttttgga ccgcaggact ctggaaacgc tctgcacacc
      181 ctaaagctgg agcggcgcat ttctgatgcg ccgctccagc tttttaggtc tctaatgtat
      241 ccataagctg ccgaagctgc tccagctccg gctgacagcc atagtcctct aagtcccagg
      301 ggtccagctt ctcacagagc agcgcacgcc ccagctgccg ggccatgtcc ggatcacgcg
      361 gcgttccgat ccccagagca taacatcgaa tcaggcggca gcggcagttg acgtttccct
      421 tttccgcacc cgcctggtag agcgcaaacg cctgcttcgg gtcctgctct actccaattc
      481 cgatctcata aaaacgcccc agtgcccaaa aggcagcttc atccccggac tgctgggcct
      541 gtcggtagca gtccagcgcc tctgcgatgc ggcccgcctg ttctgcatgc cagcccatgt
      601 tatagagcgc acagacactc cctgcctccg ctgcacgctg ataccaaaac atagcctgtt
      661 cgaaatttcg gggccctccc cttccatttg ccagcatctc cgccaaattg ttcatgctgg
      721 aggcattccc cttctctgcc gcacaccggt aatattcgac tgccctggcg taatcctgtt
      781 ctgtcccgat gccgtattca taagaccagc ccaaaagccc caaggagtcg gcatccccca
      841 cttcggcgga cttgtgaaac cagatcaacg cctgcttcag gtcctctgca actccaatgc
      901 cgccctgaca gcaccggccc agttcataca tggatcggac ggaaccccgc tctgctccct
      961 gcctgtacca gcttaccgca gcttccgggt ctttttccac accttttccc ttttcatagc
     1021 accagcccag atttcccatc gcaacagtat ttccggcctg tgccgcccgc cgataccact
     1081 ccacggcctg cttccagtcc tggggaacac cgtgtccacg ctcatagcac caccccaggt
     1141 tgcacatggc gttgtcctgc tcttgttccg cagcccttcg cagccagatg actgcctgct
     1201 tgtcatcctt cccaatgccc ctgccatgga ggagccggta tccaaagctg ctcatagagc
     1261 ggggattacc cgcttccgcg cctttgcgat acagctctgt tgcctggacc tggtcctgct
     1321 ctaccccgat cccttcagaa tagcaccagg ccagatttcc catggcgcgg atgctgcccg
     1381 cctccattgc acggcggtac aaagctgcgg cctttttccc gtcctgatcc acgccctgcc
     1441 ctgtctcata cagccacccc aggcagcggc tggcttccgc gtctccggcc tcagatgcct
     1501 gttcatacca gtgcgcggca agagggaggc tggcctctac gccctcccca tgctcatagt
     1561 cccaggccag gcagtacatg gaacgtacgt ctccagactc ggctccacgc tggaaccacc
     1621 gggcggcttc ggcgtggtcc tgctccacgc cttttccgtt gtcatagcac caagccaggt
     1681 tgcccatggc ccggccgctc ccggcctcgg cgctccgcag ataccactcc gccgccttct
     1741 cccagctctg ctccacgccc tgacccgcct catagagcca gcccaggttg cacatagcat
     1801 cgctgtcccc tccgtccgca gagcggcggt accagtaggc ggcctgagca tagtcctgtc
     1861 cgatcccccg gcccttctca cagcaccatc ccagatcata ctgggccgtg gagtccccct
     1921 tctgagcccc cagggtccac cagcggaccg cctcctggtc attctgaggg accccattcc
     1981 ccttcaggca catctgtccc agctcgcagg ccgcggactc ggcccccagc tcccatgccc
     2041 ggaccagcag gtcccaggcc cggtctgggt cccggggcat cccggtccca ttgaggcagc
     2101 agatccccag aagcagagcg gcccggctgt gcccctgctc cgccgcctgc gaatagagct
     2161 ggaccgcgcc gtccgcgtcc tgctccaccc attttccgtg ttccatcagc cagcccagac
     2221 agcactgtcc gtccgcatcc cctgcctgag ccgcacggcg gtaccactgt acggcctgct
     2281 tgggggagat ctccgtcccg acgccaaact catagtgccg ggccagctcc gcctggctgg
     2341 aggcgtcccc ctgaagggcg gaggtccgca gccattccag ggcagccttt tggtcctggg
     2401 gcagtcccag cccctgttcc agacagcggg ccagcagcct ctgcccccgg gaggagc
//
LOCUS       DBMDSJ010000267         2452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10112_length_2452_cov_2.140791, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000267 DBMDSJ010000000
VERSION     DBMDSJ010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10112_length_2452_cov_2.140791"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            46..1167
                     /locus_tag="ACZPAQ_06850"
     CDS             46..1167
                     /locus_tag="ACZPAQ_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR0903741.1"
                     /translation="MISLARLFSLMKREAVLSAAAALALLSMLWMPPDGAYLSYVDWR
                     TMAQLFCLMAVMAGLRELGVFSRLGRQLLCRVRSARQLECVLVFLCFFTSMAITNDVA
                     LLTFVPFSLEVLTLAGREERAVPVVVFQTVAANLGSMATPIGNPQNLYLYAQSGQGLG
                     DFLALMLPYTLLSGLMLLLCLLPGRSAPLAGLAPGGAEPSLRRRRTGMYLLLFLASVS
                     SVAKLLPVWLLTAAVVVCVLAADRRVLRRVDWSLLLTFAAFFVFIGNMGRVPAFRSLL
                     EQAISGHELVTAVCASQVISNVPAALLLSGFTSEWDALIVGTNLGGLGTLIASMASLI
                     SYKFFVREHPQKRGAYLLFFTGANLLFLAVLLPAALLLG"
     gene            complement(1257..2198)
                     /locus_tag="ACZPAQ_06855"
     CDS             complement(1257..2198)
                     /locus_tag="ACZPAQ_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR0903742.1"
                     /translation="MAEYILNLLPVTEEEKAAFQEAAPRAVHVCAGRRTVTAEQLEQA
                     TILFGWPRPADLSRAPRLKWFQSMWAGTDEYLAPGVLPEGVVLTSSAGSNSQSVAEHA
                     LACLLALCRRLPQCRDQQRERLWTDPGKMKTLSGATVLVAGAGHIGSWFAGLCRGLGA
                     HTVGLKRDVSRSVEGFDRLAVMEELDGLLPQADVVALMLPHSPQTEGLMDARRLRLMK
                     EDAILLSMGRGSVLDQEALAAVMGEGRLWGAALDVTVPEPLPQDSPLWTIPNLIITPH
                     SAGGMRLEVTRANCVRQALDNLNRWQAGKPLENLVRR"
BASE COUNT          347 a          848 c          783 g          474 t
ORIGIN      
        1 accccccttt gacctgaccg gggtggcggg ggagggagga gcactgtgat atccctggcc
       61 cgtctgtttt ccctgatgaa gcgggaagcc gttctgtccg ccgccgcggc gctggctctg
      121 ctgtccatgc tgtggatgcc gcccgacggg gcctatctgt cctatgtgga ctggaggacc
      181 atggcccagc tgttctgcct gatggcggtg atggctgggc tgcgggagct gggggtcttc
      241 agccgcctgg gccggcagct gctctgccgg gtcaggagcg cccgccagct ggagtgtgtg
      301 ctggtctttc tgtgcttttt caccagcatg gccatcacca acgatgtggc cctgctcacc
      361 tttgtcccct tttccctgga ggtgctgaca ctggccggac gggaggagcg ggcggtgccg
      421 gtggtggtct ttcagactgt ggctgcaaac ctggggagca tggccacccc catcggcaat
      481 ccccagaacc tgtatcttta cgcccagtcc ggccagggac tgggggattt tctggccctg
      541 atgctgccct acacccttct gtccggcctg atgctgctgc tctgtcttct gccggggcgg
      601 agcgcccccc tggccggtct ggcccccggc ggggcggagc cctctctgcg ccggcgtcgg
      661 accgggatgt acctgcttct gttcctggcc tcggtctcct ctgtggccaa gctgctcccg
      721 gtgtggctcc tcaccgccgc cgtggtggtg tgtgtcctgg ctgccgaccg gcgggtgcta
      781 cgccgggtgg actggtccct gctgctcacc ttcgccgcat ttttcgtctt tattggaaac
      841 atgggccgcg tacccgcctt ccgctcgctg ctggagcagg ccatctccgg ccacgagctg
      901 gtcaccgccg tgtgcgccag ccaggtcatc agcaacgtgc ccgccgcctt gctcctctcc
      961 ggcttcacct cggagtggga tgcccttatc gtggggacca acctgggcgg gctggggacc
     1021 ctgatcgcct ccatggctag cctgatctcc tataagttct tcgtccggga gcacccccag
     1081 aagcggggag cctatcttct cttctttacc ggggccaacc tgctctttct ggccgtcctg
     1141 ctcccggcgg ccctgctgct gggctgaccg ggccgcgccc atgggaaggg actgactggt
     1201 ccacttttct aaaaacaggg aggcgcgctg attcatcagc gcgcctcaat ttgtttctat
     1261 ctccgcacca gattttccag gggtttccca gcctgccagc gattcaggtt gtccagcgcc
     1321 tgccggacgc agttggcccg ggtgacctcc aggcgcatcc cgcccgcgga gtggggggtg
     1381 atgatcaaat tggggatcgt ccacaggggg ctgtcctggg gcaggggctc tgggaccgtg
     1441 acatccaggg cggcgcccca caggcgtccc tctcccatga cggccgccag cgcctcctga
     1501 tccagcacgg agccccgccc catgctcagc aggatggcgt cctccttcat cagccgcagc
     1561 cgccgcgcgt ccatcagtcc ttccgtctgg ggggagtggg gcagcatcag ggccaccacg
     1621 tcggcctggg gcagcaggcc gtccagctcc tccatcaccg ccagccgatc aaagccctcc
     1681 accgaccggg agacatcccg cttcagcccc acagtatggg cacccagccc ccggcacagt
     1741 ccggcaaacc aggagccgat gtgtcccgcc cccgccacca ggacggtggc accggagagg
     1801 gtcttcatct ttcctgggtc cgtccagagc cgctcccgct gctgatcccg gcactggggg
     1861 aggcggcggc acagggccag caggcaggcc agggcgtgct ccgccaccga ctggctgttg
     1921 gagccggcgg aggaggtgag gaccacccct tccggcagga cgccgggggc cagatactcg
     1981 tcggtccccg cccacatgga ctggaaccac ttcaggcggg gggcgcggct caggtcggcg
     2041 ggccggggcc acccgaaaag gatggtggcc tgttccagct gctccgctgt cacagtgcgc
     2101 cgcccggcgc agacgtggac ggcccggggg gctgcctcct gaaaggcagc tttttcctcc
     2161 tccgtcaccg gcagcaaatt cagaatatac tcagccatgg gggagcctcc ttttttcaaa
     2221 agacagatga tattgattat accccagtcc ctcccctgat ggcaagagcg gcacgttccc
     2281 tcctccgacg gtacggccta tgctcacttc agagggatgc agaactcaca ccggtgcagg
     2341 gccacctttt cggcagagta gcgctccaaa acggggctgg cgcggtccac cggcagcccg
     2401 gcggtcaggc gctccagatc ttcccagaac cgggagacct cccgctccgt at
//
LOCUS       DBMDSJ010000268         2445 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10143_length_2445_cov_1.975256, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000268 DBMDSJ010000000
VERSION     DBMDSJ010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10143_length_2445_cov_1.975256"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(436..1602)
                     /locus_tag="ACZPAQ_06860"
     CDS             complement(436..1602)
                     /locus_tag="ACZPAQ_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR0903743.1"
                     /translation="MLDRLRSHSFSYQAPVLVCLLAILLLLVLPTGFEGALIYQEAER
                     CTARVLETDNSAIIDTGLVRSGEQRCQLEILDGRFQGRTVTGINMLNGSLEQDKLFSP
                     GDRAQVVVSHNGDTITNVTMSDHYRLGWEALMAAGFALFLILFAGRTGVRAIASFVLT
                     VLCLWKLLVPLYLKGWNPIWVGLAITLFLTILIIALVYGFDRRCWAAVSGSFLGILVT
                     CVLGILFTDLFQIHGAVMSYSESLLYSGYAHLSLTQIFMASIFIGASGAVMDLSVDIT
                     SAVHEVVEKRPDLSWQEAARSGLNVGRAAMGTMTTTLLFAYSGGYIALLMVFMAQGTP
                     VDHILNYKYVAAEIIHTVIGSFGLVTVAPFTALCAGLFLARRGGHPQPQKESDG"
     gene            complement(1737..>2445)
                     /locus_tag="ACZPAQ_06865"
     CDS             complement(1737..>2445)
                     /locus_tag="ACZPAQ_06865"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR0903744.1"
                     /translation="VAKGIEVLDNDTGFFMMCEGGKIDWACHANDAAASIHDTQALAD
                     AVQVAIDFAKEHADETLILVTGDHETGGLTIGFAGTDYDTYLDLLENQKISFAKYDSD
                     YVASYKANKTPFETVLADIEKLFGLKAQGEEGDKLVLTAYEMEQLRAAYEKSINGTAS
                     SQYDQAEYVLYGTYEPLSVTITHIINNKSGISFTSYSHTGLPVAVLAEGAGSEMFNGY
                     YDNTDIYNKLADLLNIA"
BASE COUNT          498 a          691 c          825 g          431 t
ORIGIN      
        1 ggcggcattg acgatggcgt ccacctgcaa tttggtgatg tcccctgtga cagcgcgcag
       61 ttccatggaa agccctcctg ttttctgaat tttgggatgc ttctagtgta ccacgcccag
      121 ccccggagca caaggaagag gcgcgtatta tgtaagtctc agcggcacca tcagggcgcg
      181 ggcattgact cccgacggcg cacaccgcat gccactcaga gcaggtcacg ttctgaacgt
      241 cacaaaagtg atcaaagctg gagatgcccg gagcggctgt gtcaccactt cacagcacag
      301 gggcccgcag gatgccccgg tgcaagtacc gcagaaacca gcatcttcag gccgcagagg
      361 agggactcag aagcccggca gcatcggcag gcattcagaa gggcactccc atggagagca
      421 gggggctccg gcagatcatc catctgactc cttctggggc tgaggatggc cgccgcgccg
      481 ggccaggaaa agtccggcgc acagggcggt gaagggggcc acggtgacca gaccgaagga
      541 gccgatgacg gtgtggatga tctccgccgc cacatacttg tagttcagga tgtggtcaac
      601 cggggtgccc tgggccatga agaccatgag cagggcaatg tagccgccgg agtaggcgaa
      661 gagcagggtg gtggtcatgg tgcccatggc ggcccggccc acgttgaggc cggagcgggc
      721 ggcctcctgc cagctcagat cgggccgctt ctccaccacc tcgtggacgg cggaggtgat
      781 gtccacgctc aggtccatca ccgcgccgga ggccccgatg aagatggagg ccatgaagat
      841 ctgggtcagg ctcaggtggg cgtagccgga gtagagcagg ctctcagagt aggacatcac
      901 ggccccgtgg atctggaaca ggtcggtaaa gaggatgccc agcacacagg tgaccaagat
      961 gccaagaaag gagccggaca ccgccgccca gcaccgccgg tcaaagccgt acaccagagc
     1021 gatgatcaaa atggtgagaa agagggtgat ggccagcccg acccagatgg ggttccagcc
     1081 cttgaggtag agggggacca ggagcttcca caggcagagc acagtcagca cgaaggaggc
     1141 gatggcccgt accccggtgc gcccagcgaa cagaatgagg aagagggcaa agccggcggc
     1201 catcagggcc tcccagccca ggcggtagtg gtcgctcatg gtgacattgg tgatggtgtc
     1261 cccgttgtgg ctcaccacca cctgggccct gtccccggga gagaagagct tgtcctgctc
     1321 cagagagccg ttgagcatgt tgatcccggt gaccgtcctt ccctgaaatc tgccgtccag
     1381 gatctcaagc tggcagcgct gctcaccaga gcggaccagg ccggtgtcga tgatggcgga
     1441 gttgtctgtc tccagcaccc gggcggtgca gcgctccgcc tcctggtaga tcagagcgcc
     1501 ctcaaagccg gtgggcagga ccagaaggag caggatggcc agcaggcata ccagcacagg
     1561 ggcttggtag gaaaaagaat gggatctgag gcggtcgagc atggatgaaa actccttttc
     1621 gcatgatttc gggggacagg aaaatgtctc actccagaac acaggggcgg cccgcccccg
     1681 gcaaatatgc cggaggcggg cccggaaggg gcgctggagg ggttgcctgg accagctcag
     1741 gcgatgttca acaggtcggc cagcttgttg tagatgtcgg tgttgtcata gtagccgttg
     1801 aacatctcgc tgcctgcgcc ctcggccagg actgccacgg gcaggccggt gtggctgtaa
     1861 gaggtgaagg agatgccgga cttgttgttg atgatgtggg tgatggtgac agagaggggc
     1921 tcgtaggtgc cgtagagcac atactccgct tggtcgtact ggctggaggc ggtgccgtta
     1981 atgctcttct cataggcggc ccgcagctgc tccatctcat aggcggtgag caccagcttg
     2041 tcgccctcct cgccctgggc cttcaggcca aagagcttct caatgtcggc cagcacggtc
     2101 tcaaaggggg tcttgttggc cttataggag gccacatagt cgctgtcgta cttggcgaag
     2161 gagatctttt ggttctccag cagatccaga taagtgtcgt agtcggttcc ggcgaagccg
     2221 atggtcaggc cgccggtctc atggtcgccg gtgaccagga tgagggtctc gtcagcatgc
     2281 tccttggcga agtcgatggc cacctgtacc gcatcggcca gggcctgggt atcgtggatg
     2341 gaggcggcgg cgtcgttggc gtggcaggcc cagtcgatct tgccgccctc gcacatcata
     2401 aagaagccgg tgtcgttgtc cagcacttcg atgcccttgg ctaca
//
LOCUS       DBMDSJ010000269         2430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10211_length_2430_cov_2.022757, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000269 DBMDSJ010000000
VERSION     DBMDSJ010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10211_length_2430_cov_2.022757"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /locus_tag="ACZPAQ_06870"
     CDS             complement(<1..1403)
                     /locus_tag="ACZPAQ_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903745.1"
                     /translation="MRFAKTVTALALACSLALAIPTFAAPAAEEQAGRQEDLELLYQT
                     IRDRHPGPFTNTPEQEFLARIAQIEARIDTEDSMSFGLDLQSLAALIGDSHTTTNLNS
                     ILSSGHMFPVSVKWMDGDWVLYQVDASHQGALGQQVTSVNGFSMDQVQEKMAPLISAD
                     NEIKLRRQLSQVFGHQEIMVYCGLLKPEEDLTLTLENGTSITLSPLSMQDQSGWPEIN
                     GLSQQRQGSAPTDYQKGTYYFSLPLDSRTYYIQYNQCMEDPELPMETFTAQVMADLEQ
                     GSYSVLLLDLRNNGGGSDGVIIPLLMELAPLVRNGELELWGLVGEATFSSASINALEI
                     REMGGGLAGEPTSGSVDHFGSTGSFTLPNTGVRVSMSTKFIDTGTLLECAAGLGVEPI
                     QPDVTVYQTLEDYLAGQDTLVDALCARTEPFQPQERPDAPLSRGRLTELLRQAAEQAG
                     LDTQAPMQSLSDLLPCA"
     gene            complement(1684..>2430)
                     /locus_tag="ACZPAQ_06875"
     CDS             complement(1684..>2430)
                     /locus_tag="ACZPAQ_06875"
                     /inference="COORDINATES: protein motif:HMM:NF025091.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR0903746.1"
                     /translation="MGGQAAAASLLGVPDLGLVCGCEQTKHAQDLASCVDLPFLIDAD
                     TGYGNELNVRRTMRDLEAAGICGAHFEDQVTPKRCGGMKGVQIETAQVFIRKIETALT
                     FRRDPNFLVIARSDAAEVEGEDEAIRRLNLAAEAGADMGFLFSSKGVFETLDSVKRAC
                     DQIKVPILYCLMEFCPEVCFTLEELRQAGVRAVIWPNGLLMRWCKAASDLIQQFKETG
                     DCKTFFDQLMPIDQCNQLLGIRDWNPSGMF"
BASE COUNT          531 a          687 c          739 g          473 t
ORIGIN      
        1 caggcacagg ggagcagatc agacagactc tgcatggggg cctgagtgtc caggccggcc
       61 tgttccgcag cctgccggag cagctcggtc agccggcccc ggctgagcgg ggcatcgggc
      121 cgctcctggg gctggaaggg ctctgtgcgg gcgcacaggg cgtccaccaa ggtatcctgg
      181 cccgccaggt agtcttccag agtctgatag acggtcacgt cgggctggat gggctccact
      241 cccagcccgg cggcgcactc cagaagggtg cctgtgtcaa taaatttggt ggacatggac
      301 acccggaccc ccgtgttggg gagggtgaag gagccggtgg agccgaagtg gtccacgctg
      361 ccgctggtgg gctcaccggc cagcccgccg cccatctccc ggatctccag ggcgttgatg
      421 ctggcggagg agaaggtggc ctcccccacc agcccccaca gctccagctc cccgttgcgg
      481 accagggggg ccagctccat cagcaggggg atgatgaccc cgtcggagcc gccgccgttg
      541 ttccgcagat ccaaaagaag gacggaatag ctcccctgct ccaggtcggc catgacctga
      601 gcagtgaagg tctccatggg cagctccggg tcctccatgc actggttgta ttggatatag
      661 taggtgcggc tgtccagggg cagagagaaa tagtaggtcc ccttctggta atcagtgggg
      721 gcgctgccct ggcgctgctg ggacaggcca ttgatctccg gccagccgga ctggtcctgc
      781 atggagagag gggagagggt gatagacgta ccgttttcca aggtgagggt cagatcctcc
      841 tcgggcttga gcagtccgca gtagaccatg atttcctgat ggccgaacac ctgggagagc
      901 tgccggcgca gcttgatctc gttgtctgcg gagatcagag gggccatctt ctcctgtacc
      961 tgatccatgg agaagccgtt gacggaggtc acctgctggc caagagcacc ctggtgggag
     1021 gcgtccacct ggtagagcac ccagtccccg tccatccact tcacagacac gggaaacata
     1081 tgtccgctgc tgagaataga gttaagattt gtggtggtgt gggaatcccc aatgagggct
     1141 gccagagact ggagatccag cccaaaactc atgctgtcct ctgtgtctat ccgggcctcg
     1201 atctgagcga tgcgggcaag gaactcctgc tccggcgtat tggtgaaggg gccggggtgg
     1261 cgatcccgga tggtctgata gagcagctcc aggtcttcct gcctgccagc ctgctcctcc
     1321 gccgcagggg cggcaaaagt gggaatggcc agggccagag aacaggccag agcgagagcg
     1381 gtaacggtct tggcaaagcg catggtgatt cctcccaatt cttgatggta ctatcctatc
     1441 aggggcgttt taaaggaaat aaaaaatttc cttaagatta tatgaaattt gggacagctt
     1501 caatccggga atccctctgt catggcccag gcggctggaa ctatcatacc tggttcgaat
     1561 ggaaggaaca agaaaaaaag ataatttgac aaccggagag tgcggcggta gaaaataagg
     1621 acgatcagaa aaaaacacca ggtcagcgcc aaatatggcg ctggcctggt gtctgaggag
     1681 aaatcaaaac ataccggacg ggttccagtc ccgaatcccc agcagttgat tgcactggtc
     1741 aataggcatg agctgatcaa aaaaggtttt gcagtcccct gtctccttaa actgctgaat
     1801 caggtcggag gcggctttgc accagcgcat cagaagacca tttggccata tgacggcccg
     1861 aacccctgcc tgacgcagct cttccagagt aaaacagacc tcagggcaaa attccatcag
     1921 gcagtacaga atgggaacct ttatttgatc gcaggcccgt ttgacagaat ccagagtctc
     1981 aaagaccccc tttgaggaga aaagaaaacc catgtctgct ccggcctctg ctgccagatt
     2041 gagacgccgg atagcctcat cctctccttc cacctcggcg gcatcggagc gggcaataac
     2101 taaaaagttg gggtctctcc gaaaggtcag agcggtctct atttttcgaa taaagacttg
     2161 ggcagtttca atttggacgc ccttcatgcc gccgcatcgc tttggcgtga cctggtcctc
     2221 gaaatgcgcg ccgcagattc cggcggcctc caggtcccgc atagtgcggc ggacattcag
     2281 ctcattccca tagcctgtgt ccgcgtcgat aagaaagggc agatccacac aggaggcaag
     2341 gtcctgggcg tgctttgtct gttcacatcc gcagacaaga cccagatccg gcacacccag
     2401 caggctggct gctgctgcct gaccgcccat
//
LOCUS       DBMDSJ010000270         2417 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10279_length_2417_cov_1.427461, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000270 DBMDSJ010000000
VERSION     DBMDSJ010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10279_length_2417_cov_1.427461"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="ACZPAQ_06880"
     CDS             <1..194
                     /locus_tag="ACZPAQ_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903747.1"
                     /translation="TMLAICCTSSQLTSIRALLPISRHGAASTRDFIFPSLAVRIVFV
                     NPFPVLCPPTALVKPGVLW"
     gene            384..1163
                     /locus_tag="ACZPAQ_06885"
     CDS             384..1163
                     /locus_tag="ACZPAQ_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903748.1"
                     /translation="MEFQNRLYELRKKAGLSQEGLADLLGVTRQAVQKWEAGSSRPDM
                     DNLAALARYFNVTLDYLVTGREPEPYAQEVPTTIINHNYYPRWHYEYKSQRTLFGLPL
                     VHVRLGDRGFGVAKGIFAVGNVAVGLFALGGISLGLFSLGGVSLGLLLALGGMSVGGI
                     AIGACAVGLAALGGGAIGLLSVGGGSFGAYAVGGGAVGTQIAIGGSASAPLAIGSQAA
                     SGALTFGPGADPAAVTAAIRQAAAAAPVWLQDLLISLALEV"
     gene            1248..1625
                     /locus_tag="ACZPAQ_06890"
     CDS             1248..1625
                     /locus_tag="ACZPAQ_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR0903749.1"
                     /translation="MIPTYEQMGDWLEEIAGEFPDAFFEELDGGIQLEEEALPDPDFP
                     PGEMYIMGEYCHDMLGRYIVLYYGSFAALLPEEDEEGWKDELFATVAHEFTHHMEETA
                     GLHALDDKDAEFLRQAREIYGDT"
     gene            1788..2291
                     /locus_tag="ACZPAQ_06895"
     CDS             1788..2291
                     /locus_tag="ACZPAQ_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0903750.1"
                     /translation="MAHRGEDLYEQHAQLVYRYLFSLCRDADLAEELCQETFCQSLRQ
                     LGSFRGDCTPQVWLCAIAKRLWYKELERRKRNVPLEEEHLAGQAAPDDPAQETEQREG
                     RLALYRAMQQLDPDTREVIHLRLAGDFSFRDIGDILDRSEVWARVRFYRGKEELARIM
                     GGDRDGT"
     gene            2281..>2417
                     /locus_tag="ACZPAQ_06900"
     CDS             2281..>2417
                     /locus_tag="ACZPAQ_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024881.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR0903751.1"
                     /translation="MEHKLDCCVVRDLLPAYLEGLTEEETTRQVEAHLEECPECRRHR
                     EA"
BASE COUNT          402 a          777 c          794 g          444 t
ORIGIN      
        1 gcaccatgct ggcgatctgc tgcaccagca gccagctcac ttccatccgc gctctcctcc
       61 cgatctcacg ccacggggcc gccagcaccc gggacttcat cttccccagt ctagcggtcc
      121 gcatcgtgtt tgtcaatcct ttccccgtgc tctgtccgcc caccgcgctt gtcaagcccg
      181 gtgtgctgtg gtaagataag ccccggcgga cgtctgtctc ttttcccgtt tttggaccaa
      241 aaagttgaaa aaattccgga tgcaaccgtt agttgtcaaa ttgtatgcct cttttggttg
      301 ccctgtctgc ctctttttgc taaggtcttc ttgccgccgg ggcgtgccgg ccggtcttat
      361 cacttgatcc aggaggagtc cctatggaat ttcaaaaccg tctctatgag ctgcggaaaa
      421 aagcagggct gagtcaggag gggctggccg atctgctggg ggtgacccgg caggcggtcc
      481 agaaatggga ggccggcagc agccggccgg acatggacaa cctggccgcc ctggcccgat
      541 atttcaatgt cacgctggac tatctggtca ccggccggga gccggagccc tatgcccagg
      601 aagtccccac caccattatc aaccacaact attaccctcg ctggcactat gagtataaga
      661 gccagcgcac cctgttcggc ctgcctctgg tccacgtccg cctgggggat cggggcttcg
      721 gcgtggccaa ggggatcttc gccgtgggca acgtggcggt tgggctcttc gccctgggcg
      781 gcatctccct gggactgttc agcctgggcg gggtctccct gggcctgctg ctggccctgg
      841 gcgggatgtc cgtgggcggc atcgccattg gggcctgtgc ggtgggactg gccgccctgg
      901 gcggcggggc catcggcctg ctgtccgtgg gaggcggctc cttcggtgcc tacgcagtgg
      961 gcggcggggc ggtgggcacc cagatcgcca tcggcggctc cgcctccgct cccctggcca
     1021 tcgggagcca ggccgcctcc ggcgctctca ctttcggccc gggggccgac cccgccgcgg
     1081 tgacggccgc catccgccag gccgccgctg cagcccctgt atggctccag gacctgctca
     1141 tctccctcgc cctggaagtc tgacccttgt ttttccccgg cgcccgtggt atcatggtcc
     1201 caaatctacc gggctgggca gcccctcccc ggggacagga gcggcctatg ataccgactt
     1261 acgagcagat gggggactgg ctggaggaga tcgccgggga attccccgac gccttctttg
     1321 aggagctgga cggcggcatc cagctggagg aggaggccct gcctgaccct gattttcccc
     1381 ccggtgagat gtacatcatg ggggaatact gccatgatat gctgggccgg tacatcgtcc
     1441 tatactacgg ctcctttgcc gccctgctcc cggaggagga cgaggagggg tggaaggatg
     1501 agctcttcgc cacggtggct cacgagttca ctcaccacat ggaggagacg gccggccttc
     1561 acgccctgga tgacaaagac gccgaatttc tccgtcaggc ccgggagatc tacggcgaca
     1621 catagcagac ggagggggcc ggcggacgcc ggccccctct ctgatatcta aaaggccgtc
     1681 ccccatcagg agcctgggcg cgtcccaaaa ttttccgctc agatcctgta acgaatgtcc
     1741 cgtccctggg accaatagac agaaggacac ggaaggaggc gaccgccatg gcccaccggg
     1801 gtgaagacct gtacgaacag cacgcccagc tggtataccg ctatctcttc tccctgtgcc
     1861 gggacgctga cctggcggag gagctgtgcc aggagacctt ctgccagtcc ctccgacagc
     1921 tgggctcctt ccgcggggac tgcacgcctc aggtatggct gtgcgccatc gccaagcggc
     1981 tgtggtacaa ggagctggag cggcggaagc ggaacgttcc cctggaggag gagcacctgg
     2041 ccggtcaggc cgcccccgac gatccagcgc aggagacgga gcagcgggag ggccggctgg
     2101 ccctgtaccg ggccatgcag cagctggacc cggacacccg ggaggtcatc catctgcggc
     2161 tggccggtga cttctccttc cgagacatcg gggacatcct ggaccggagc gaggtatggg
     2221 ccagggtccg gttctaccgg ggaaaagagg agctggctcg gatcatggga ggtgacaggg
     2281 atggaacata aactggactg ctgcgtggta cgggacctgc tgcccgccta cctggagggg
     2341 ctgacagagg aggagaccac ccggcaggtg gaggcccacc tggaggagtg tccggagtgc
     2401 cgtcggcacc gggaggc
//
LOCUS       DBMDSJ010000271         2406 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10324_length_2406_cov_2.123644, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000271 DBMDSJ010000000
VERSION     DBMDSJ010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_2406_cov_2.123644"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            173..1126
                     /gene="hprK"
                     /locus_tag="ACZPAQ_06905"
     CDS             173..1126
                     /gene="hprK"
                     /locus_tag="ACZPAQ_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR0903752.1"
                     /translation="MTETQNSVRLGEIIKEFHLELLHEGEDYQNIPLRTLDVNRPGLP
                     LSGFFEHFDTKRLLVMGLTETTYVSGMSEERRRESFDRLLSYPVPALIYTRGIDPFPE
                     CMEMAQKYGRTVLRTQEQTSAFMSSLIGSLYNHLAPRITRSGVMMEIYGEGVLFQGES
                     GVGKSEVAIELIKRGHRIIADDAVEIKETNHGTLVASAPELIRHYMELRGIGVIDVRR
                     LFGMGAIKMNQEIDMVIKLEPWDDTAVYDRLGLENTYTEILGVQLPALTIPVKPGRNL
                     ASIVEVAAMNNRNRKMGHNAALELTRRMDQHFARKMGVGDE"
     gene            1128..2075
                     /locus_tag="ACZPAQ_06910"
     CDS             1128..2075
                     /locus_tag="ACZPAQ_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012692.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR0903753.1"
                     /translation="MYIGLDIGGTNMVAGLTNGAGKIINKVSRPVNKAWDPAELCRQS
                     AQLAQQAAEVGGFAADQIKAVGLGLPGLVDNKTGYVIQTPNMPFDNTPIREMFQAEWD
                     VPVYLGNDANCAAIGEYWAGAAKDCDPAVVVTLGTGIGGGLVVGGKLYTGFAQSGLEV
                     GHMIIRPNGVLCGCGNRGCWEQYGSATALIRIAQEAMERDPGSVMWELCQGDRFRVDG
                     RIPFQAARLGDLSAKKVLHRYLEGLSIGLINLVNILQPEIICLGGGISNAEDDLLLDP
                     LRGLVLAGCFDKSKPPRLERASLGNDAGVVGAAMLYKML"
BASE COUNT          491 a          699 c          803 g          413 t
ORIGIN      
        1 ggccgcccct ggaaatttta cgggcacgct ctggcagttt tgacgaaaaa ggagagggca
       61 gcatctcctg cctgtgtaaa ataggacaag aaaaaacagc tgttctcttg cgtgccgggt
      121 ggaaatgagc tatactaatc cagaaccaga atataggaga ggggtgcacc ctgtgacaga
      181 gacccaaaac agcgtccgtc tgggcgagat catcaaggaa ttccacctgg agctcctcca
      241 cgagggagag gactaccaga atatcccgct gcggaccctg gacgtaaacc gccccggact
      301 tccgctgtcc ggcttttttg agcattttga caccaagcgc ctgctggtga tgggactgac
      361 cgagactacc tatgtcagcg gcatgagtga agagcggcgc cgggagagct ttgaccgact
      421 gctctcctac ccggtgcctg ccctgatcta tacccggggc atcgacccgt tcccagagtg
      481 tatggagatg gcccagaaat acgggcgcac cgtgctgcgc acccaggagc agacctcggc
      541 ttttatgagc tctctcatcg gcagtctcta caaccatctg gctccccgga tcacccggtc
      601 aggcgtgatg atggagatct acggcgaggg ggtgctcttc cagggagagt ccggcgtggg
      661 caagagcgag gtggccatcg agctgatcaa gcgcggccac cgcatcatcg cggacgacgc
      721 ggtggagatc aaggagacca accatggcac cctggtggct tccgccccgg agctgatccg
      781 ccactacatg gagctgcggg gcatcggggt catcgatgta cgccgcctgt tcggcatggg
      841 ggccatcaag atgaatcagg agatcgatat ggtcatcaag ctggagccct gggatgacac
      901 cgcagtctat gaccgcctgg gcctggagaa cacctacacc gagatcctgg gggtgcagct
      961 gcccgccctg accatcccgg tgaagccggg ccggaacctg gccagcatcg tggaggtggc
     1021 ggccatgaac aaccgcaacc gcaagatggg ccacaacgcg gcgctggagc tgaccagaag
     1081 aatggatcaa cattttgccc ggaagatggg tgtgggagat gaatgagatg tatatcgggc
     1141 ttgacatcgg cggcaccaac atggtggccg gactgaccaa tggagcggga aagatcatca
     1201 acaaggtgag ccgtccggtg aacaaggcgt gggacccggc agagctgtgc cgccagtccg
     1261 cccagctggc ccagcaggcg gcggaggtgg ggggcttcgc cgcagatcag atcaaggcag
     1321 tgggcctggg cctgcccggt ctggtggaca acaagacggg ctatgtgatc cagactccca
     1381 acatgccctt tgacaacact cccatccggg agatgttcca ggcggagtgg gacgtgcccg
     1441 tctatctggg caacgacgcc aactgcgccg ctatcggtga gtactgggcc ggcgcggcca
     1501 aggactgcga ccccgcagtg gtggtcaccc tgggcaccgg catcggaggc ggcctggtgg
     1561 tgggcggcaa gctgtacacc ggcttcgccc agtccgggct ggaggtgggc cacatgatca
     1621 tccggcccaa cggcgtgctg tgcggctgcg ggaaccgggg atgctgggag cagtacggct
     1681 ccgctactgc cctgatccgt attgcccagg aggccatgga gcgggacccg ggcagcgtga
     1741 tgtgggagct gtgccagggg gaccgcttcc gcgtggacgg acgtatcccc ttccaggctg
     1801 cccggctggg ggacctgtcg gcaaagaagg tgcttcaccg ctatcttgag gggctgtcca
     1861 tcggcctgat caacctggtg aacatcctcc agccggagat catctgcctg ggcggcggca
     1921 tcagcaacgc agaggacgac ctgctgctgg accccctgcg ggggctggtg ctggcgggct
     1981 gctttgacaa gtccaagccg ccgcgcctgg agagagcctc cctgggcaac gacgccggag
     2041 tggtaggggc tgccatgctg tacaagatgc tgtaagggcc cggctcccgg ccgggagagc
     2101 agcggtggaa caagaacagg gcggcctccg cagatgcgga ggccgcctcg gcttgtggaa
     2161 aaagctctcc tgtgaggaat tccgccaccc gcaggcgacg gaataaaagt ttgatttcgt
     2221 catttccaag ggcgtgtacc tcgggaatga cagtctctcc gcccattgcc tggctggctc
     2281 ctgagaacgt ccgggttcag aggacaaaat cctcgtcctt cagcctggag aaatccgcct
     2341 tagccgggcg gggagggacc cgtttcaatg ggtcgtactt gaaggatcgg cagctggaac
     2401 cggcag
//
LOCUS       DBMDSJ010000272         2397 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10364_length_2397_cov_2.284408, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000272 DBMDSJ010000000
VERSION     DBMDSJ010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10364_length_2397_cov_2.284408"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="ACZPAQ_06915"
     CDS             <1..217
                     /locus_tag="ACZPAQ_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR0903754.1"
                     /translation="VITGVEGAMLAPLFARCFRITDPVAEGVAIGASSHAVGTSKALE
                     IGPLQGAMSSIAICVCGIFTSVLALFF"
     gene            complement(314..>2397)
                     /locus_tag="ACZPAQ_06920"
     CDS             complement(314..>2397)
                     /locus_tag="ACZPAQ_06920"
                     /inference="COORDINATES: protein motif:HMM:NF021507.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR0903755.1"
                     /translation="GVGDVMVRDGQITDSTAWTYVEKGRDPRSALGVREDGTLVLYAV
                     DGRRSGYSGGLSQLDLAEEMLSQGCVWAVNLDGGGSTAISVWVPGQEGPSIVNIPSDG
                     KPRACATYLLLVSRDEGDGDPDRLVLKNDGLVVLTGTSVDLGETAVLDSGLNILRDDP
                     GEVEISSLDDLGRVEDNIYTAGSDAGTDTLELWSPDLDVEGTAQIHVVDHLTGLTVSR
                     ENSTDALTSLTLEPGDQVQLTAQGTYWSRAAMRDVGAISWTVEGSIGSITEDGLFTAE
                     GPGGASGTLTASAGGQSQTIMVSLENSHEDVPEGHWAYTAVDYCYANGLVGGISDTEF
                     GPDLHIRRGDFLLMLYGAAGKPAVTSPASFTDVSPSDYYYTAVSWAQANGLATGVGDG
                     TLAPASDVTREQAFTILNQALPLLGITCQPGALTVLDQFGDRASLSDWAAQHAATLVA
                     YQIVGGDTDGNLNPQAFLTRAEMAALLYKLSNYTPPADLDPGDLVQPEEDPQPGGTAA
                     PGQAEGPGQTADPDQPEDPGQTSQPEEPAVPVEGITLDTGELTLQAGSSYHFTPTLSP
                     EGAAGTVVWTSSVPTVAPVTSDGTITNLNTTGGQVSVTITASCGDACTSAVVLCDTAA
                     SAGTVRGAENGLNVRSGPSTSHSVVGYLREGGRVILLAPPEDGWLHVSYLTQSGQAAI
                     GYVSLDYIVLD"
BASE COUNT          391 a          740 c          875 g          391 t
ORIGIN      
        1 ggtcatcacg ggagtggaag gagccatgct ggcccctctg tttgccagat gcttccgcat
       61 caccgacccg gtggcggagg gagtagccat aggcgccagc agccacgccg tgggtacctc
      121 caaggccctg gaaatcggcc ccctccaggg ggccatgagt tccatcgcca tctgcgtgtg
      181 tgggatcttc acctcagtgc tggcgctgtt cttctgaaga aggcagcgga gctggacaca
      241 gagaggatag ataagaaagc acagaaggcc gccggcatcg ccggcggcct tctgctgttt
      301 atttgccttg agatcagtcc agtacgatat agtccaggga gacatagccg atagcggcct
      361 ggccggactg ggtgaggtag gagacgtgga gccagccgtc ttccggcgga gcgaggagga
      421 tgaccctgcc tccttcccgc agatagccca ccacagagtg ggaggtgctg gggccggaac
      481 ggacgttcag gccgttctcc gccccgcgga cggtgccggc ggaggcggcg gtgtcgcaga
      541 ggaccacagc gctggtgcag gcgtccccgc aggaggcggt aatggtgacg ctcacctgcc
      601 cgccggtggt gttcaggttg gtgatggtgc cgtcgctggt gactggcgcc acagtgggca
      661 cgctgctggt ccagacgacg gtgccggcgg ctccctcagg ggagagcgtg ggggtgaagt
      721 gatagctgga gccggcctgg agggtcagtt cgccggtgtc cagggtgatg ccctccacgg
      781 ggacagcggg ctcctccggc tggctggtct gtcccgggtc ctccggctgg tccggatcag
      841 cagtttggcc cgggccctct gcctggccgg gggcggcggt gccgcccggc tgagggtcct
      901 cctccggctg gaccaggtcg ccggggtcca gatcggcagg aggcgtatag ttggacagct
      961 tatacagcag agcggccatc tcagcgcggg tgaggaaagc ctgaggattc aggttgccgt
     1021 cggtgtcgcc gcccacgatc tgataggcca ccagggtggc agcgtgctgg gcagcccagt
     1081 cggagaggga ggcgcggtcg ccgaactgat ccagaacggt gagagcgccg ggctggcagg
     1141 tgatgcccag gagaggcagg gcctggttca gaatggtgaa agcctgctcc cgggtgacat
     1201 cagaggcggg agccagggtg ccatccccca cgccggtggc cagaccgttg gcctgggccc
     1261 aggacacagc ggtgtagtag tagtcggagg gggatacgtc agtaaaggag gcgggggagg
     1321 tgacggccgg cttacccgcc gcgccgtaga gcatcagcag aaagtcgccc cggcggatgt
     1381 gcaggtcagg gccgaactca gtgtctgaga tgccgcccac caggccgttg gcatagcagt
     1441 agtccacggc ggtgtaggcc cagtgcccct cgggcacgtc ctcgtgggag ttctccaggg
     1501 agaccatgat ggtctggctc tgcccgccgg cagaggcggt gagggttccg gaggcgcccc
     1561 cggggccctc agcggtgaac agtccgtcct cggtgatgga gccgatgctg ccctccaccg
     1621 tccaggagat ggctcccacg tcccgcatgg cagcccggga ccagtaggta ccctgggcag
     1681 tgagctggac ctggtctcca ggctccaggg tgagggaggt gagggcgtcg gtgctgttct
     1741 cccgggagac ggtgaggccg gtgagatggt ccactacgtg gatctgggcg gtgccctcca
     1801 catccagatc cggggaccag agctccaggg tatcggtgcc cgcgtcactg ccggcggtgt
     1861 agatgttgtc ctccacccgg cccaggtcgt ccagggagga gatctccacc tcgcctgggt
     1921 catcccgaag gatgttcaga ccgctgtcca gcacggccgt ctcccccagg tccacagagg
     1981 tgccggtgag gaccaccagc ccgtcatttt tcagcaccag ccggtccgga tcgccgtcac
     2041 cctcatcccg ggagaccagg agcaggtagg tggcgcaggc ccggggcttg ccatcggagg
     2101 ggatgttgac gatggagggg ccctcttggc cggggaccca gacactgatg gcggtggagc
     2161 cgccgccgtc caggttgacc gcccagacgc agccctggga gagcatctcc tccgccagat
     2221 ccagctggct cagtccgccg gagtagccgc tcctgcggcc gtccacagcg tacaggacca
     2281 gggtgccgtc ctcccgcacg cccagggcgg agcgggggtc gcggcccttt tccacatagg
     2341 tccaggcagt ggagtcagtg atctgtccgt cccggaccat cacatcgccc acgccgc
//
LOCUS       DBMDSJ010000273         2396 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10366_length_2396_cov_1.823965, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000273 DBMDSJ010000000
VERSION     DBMDSJ010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10366_length_2396_cov_1.823965"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="ACZPAQ_06925"
     CDS             complement(<1..460)
                     /locus_tag="ACZPAQ_06925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0903756.1"
                     /translation="MNRFLSLALAALLLAPCVSAADLPASAAPSGPETAAASAVLMEK
                     ESGAVLYEKDARQKLEPASVTKIMTLLLIMEALDSGRIARDDMVTVSAYAAGMGGSQV
                     YLKEGEQMSVSELLKCITVVSGNDAAVAMAEYLSGSEAAFVAEMNLRAREL"
     gene            658..975
                     /locus_tag="ACZPAQ_06930"
     CDS             658..975
                     /locus_tag="ACZPAQ_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HIR0903757.1"
                     /translation="MSKAEEKRQREQLIRQVRRLTRARVNDAVKLAYLSGEALEAIDG
                     LDLTAMTEFKRAGNGAVEIKFTDRLKALEKLMELIRENRETEAEELLRTLGGTAEEPE
                     WER"
     gene            963..2219
                     /locus_tag="ACZPAQ_06935"
     CDS             963..2219
                     /locus_tag="ACZPAQ_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HIR0903758.1"
                     /translation="MGALRFSPKQRQVLTWWREGSPHWEKSAIICDGAVRSGKTLCLG
                     LSFFLWAMARFQGRRFALCGRSMEGVRRNLTGEVLPLLRGMGFQCREQLTRGEVTVSL
                     GRRRNIFCLFGGGDEGAAARIQGVTLAGALLDEAVLMPRSFVEQCCARCSVQGARLWF
                     SCNPEGPGHWFYQEWVQKAGERNALYLHFTMEDNPGLSPQVRERYRTMFQGTFYRRFV
                     LGEWTAAAGLVYDFFSREMVCEPPEGRAAEWYISCDYGTVNPTSMGLWGRWGEVWYRM
                     EEFYYDSRREGRQKTDSEYADALEALADGRPIREVVADPSAASFLECLRRRGWQVRRA
                     ENEVLSGIRTTAELLRTGRLVICPGCGDAIREFGLYRWDTSAGGRDQVCKEHDHAMDD
                     IRYFAVTVAAKERGGSWAGSVERRIF"
BASE COUNT          427 a          653 c          932 g          384 t
ORIGIN      
        1 ccagctcacg ggcccggagg ttcatctcgg caacaaaggc ggcctcgctg ccggaaagat
       61 actcggccat ggccacagcg gcgtcattgc cggagaccac agtgatgcac ttgagcagct
      121 cggagacgga catctgctcc ccctccttca ggtagacctg ggaaccgccc atgccggcgg
      181 cataagcgga gacggtcacc atgtcgtccc gggcaatgcg gccgctgtcc agggcttcca
      241 tgatgagaag cagggtcatg atcttggtga cgctggcggg ctccagcttc tggcgggcgt
      301 ctttttcgta gaggacagcg ccgctctcct tctccatcag caccgcagag gcggcggctg
      361 tctccggccc ggagggggcg gcggaggcgg ggaggtcagc agcagataca cagggcgcca
      421 gcagcagggc ggccagcgcc aaagaaagga aacggttcat ggcgggtccc tccaaatcca
      481 aatgtttctg tgaaaaggat gtggaggggc gaggacttct atacgccgca gcggaccggt
      541 atttttttct gcgcaaatcg ttgcatgatg caaaaatttt tcaaaaattt ttggcctgcg
      601 tggacgggtc tgtacaactt tttccgaagt cggaccaggg gtgaggaggg tgatggggtg
      661 agcaaggcgg aagaaaagcg gcagcgagag cagctgatcc ggcaggtgcg acgactgact
      721 cgggcgagag tgaatgacgc cgtcaagctg gcctatctgt ccggggaggc gctggaggcc
      781 atcgacgggc tggacctgac ggctatgacg gagttcaagc gggcggggaa cggagcggtg
      841 gagatcaagt tcaccgaccg gctcaaggcc ctggaaaagc tgatggagct gatccgggag
      901 aaccgggaga cggaagcgga ggagctgctg cggaccctgg gcgggacggc ggaggagccg
      961 gaatgggagc gctgaggttc tcccccaaac agcggcaggt gctgacctgg tggcgagagg
     1021 ggtctcccca ctgggagaag agcgccatca tctgcgacgg cgcggtgcgc agcggtaaga
     1081 ccctgtgctt aggcctgtcc ttcttcctgt gggccatggc ccgcttccag gggcggagat
     1141 tcgccctgtg cggccggagc atggaggggg tgcggcggaa cctgacggga gaggtgctgc
     1201 ccctgttgag agggatggga ttccagtgcc gggagcagct gacccggggg gaggtgacgg
     1261 tgtccctggg gcggcggcgg aacatcttct gcctgttcgg cggaggggac gagggggcgg
     1321 cggcacgcat ccagggggtg accctggcgg gggcgctgct ggacgaggct gttctgatgc
     1381 cccgctcctt tgtggagcag tgctgcgccc ggtgctcggt ccagggggcc aggctgtggt
     1441 tctcctgcaa cccggagggg ccgggacact ggttctatca ggagtgggtc cagaaggccg
     1501 gggagcgcaa cgccctttac ctccacttca ccatggagga caacccgggc ctgtctcccc
     1561 aggtgcggga gcgataccgg accatgttcc aggggacctt ctaccgccgg ttcgtgctgg
     1621 gggaatggac cgcggcggca gggctggtat atgacttctt ctcccgggag atggtgtgcg
     1681 agccgccgga ggggcgggcg gcggagtggt atatctcctg cgactacggt acggtgaacc
     1741 ccacctctat gggactttgg gggcggtggg gggaggtgtg gtaccggatg gaggagttct
     1801 attacgactc ccgccgggag gggcggcaga agacggacag cgaatacgcc gacgcgctgg
     1861 aggcactggc ggacgggcgg cccatcaggg aggtggtggc agacccctcc gccgccagct
     1921 ttctggagtg cctgcggcgg cggggctggc aggtgcggcg ggcggagaac gaggtgctct
     1981 ccggcatccg caccacggcg gagctgctgc ggacgggccg gctggtcatc tgccccgggt
     2041 gcggagacgc catccgggag ttcgggctgt accgctggga caccagcgcc ggagggcggg
     2101 accaggtgtg caaggaacat gaccacgcaa tggacgacat ccggtatttt gccgtcacgg
     2161 tggcggcaaa ggagcgcggc ggcagctggg ccgggagcgt ggaacggagg atattttgac
     2221 cggcgggggc cggaagaggc aaaggaggct tttgcgatga agcggcggga acgaaacaag
     2281 gggcaggccc gggcggaggc ggtgcagatc cgggagagcg gacggcatcc cttcggcgtg
     2341 ctggaccggt atgtgcccct gggggcgggg gagctccagc tctaccgggc catccg
//
LOCUS       DBMDSJ010000274         2391 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10381_length_2391_cov_2.055895, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000274 DBMDSJ010000000
VERSION     DBMDSJ010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_2391_cov_2.055895"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="ACZPAQ_06940"
     CDS             <1..557
                     /locus_tag="ACZPAQ_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR0903759.1"
                     /translation="QTGALIVHYRAHFIRRLQESAPAIHRDFSGGKEELSLRYETVST
                     VTDPLAGAKTILPQLLEHQQAHRQAELDSRQCLSGPHKDDLAVEIDGNPAKTYGSQGQ
                     TRTAALSLKLAQREIFFQETGEWPVLLLDDVLSELDHRRQAFVLNRIQGGQVFISCCE
                     EEKLEGLENGKSFHIQGGRLGSGA"
     gene            607..948
                     /locus_tag="ACZPAQ_06945"
     CDS             607..948
                     /locus_tag="ACZPAQ_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903760.1"
                     /translation="MFHVLLVAPLLGLLAAYGIVVLIVLSVPFCLGGFFQLVLTIAAG
                     DRWAVWVPAGLGVLGLALSFLFLLGAIPWGGILLYWALYFLWIWLIWLVVAQIKKSVG
                     RWREKQERARR"
     gene            1039..1299
                     /gene="remB"
                     /locus_tag="ACZPAQ_06950"
     CDS             1039..1299
                     /gene="remB"
                     /locus_tag="ACZPAQ_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix regulator RemB"
                     /protein_id="HIR0903761.1"
                     /translation="MYLHLGQSVAVPHREILGIFDLDNASWAYKTREFLERAEQEGRV
                     ISVCDDLPRSFVLVGEEKGTATVYISQLSSAALLRRAESNLL"
     gene            1453..>2391
                     /locus_tag="ACZPAQ_06955"
     CDS             1453..>2391
                     /locus_tag="ACZPAQ_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0903762.1"
                     /translation="MAEELELNSTQVEHEYGASEIQVLEGLEAVRKRPGMYIGSTSES
                     GLHHLVYEIVDNSIDEALAGYCTDITVTINPGNTITVTDNGRGIPVDIQPQTGRPALE
                     VVFSVLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVQVHKNGQIYEMKFSRGKI
                     TQEMKVVGKTDHTGTTVTFKPDPEMFETLEYNYDTLHTRMREEAFLNAGLRIQTVDLR
                     PGREQEDDMCYEGGIREFVTFINRSKDPLHPDVIYMSGNREDSMAEVALQYNDSYNEV
                     IVSFANNVHTPEGGMHEEGFKRALTNVLNAYGKKIGV"
BASE COUNT          481 a          673 c          763 g          474 t
ORIGIN      
        1 cccagactgg ggcgctgatc gttcactacc gggctcactt catcaggcgc cttcaggaga
       61 gcgcccctgc catccaccgg gatttttccg ggggaaagga ggagctctcc ctgcggtatg
      121 agaccgtctc caccgtcaca gaccccctgg ccggagccaa aaccatcctg ccccagctgc
      181 tggagcacca gcaggcccat cggcaggcgg agctggacag ccgtcagtgt ctgtccggcc
      241 cccataagga cgacttggca gtggagatcg acgggaaccc ggccaagacc tacggctccc
      301 agggtcagac ccgcacggcg gccctctccc tgaagctggc tcagcgggaa atcttttttc
      361 aggagaccgg cgagtggccg gtgctgctgc tggatgacgt gctctccgag ctggaccacc
      421 ggcggcaggc cttcgtcctc aaccgcatcc agggcgggca ggtgtttatt tcctgctgtg
      481 aggaggaaaa attagaggga ctggagaatg gaaagagttt ccatatccag ggcgggcggc
      541 tgggatcagg cgcatgaccc cggtctagat gcggacagaa cagcaccata gaaaagaggg
      601 agagctatgt tccacgtcct gttggtcgct cctttgctgg ggctgctggc ggcctatggc
      661 attgtcgtcc tgatcgtact gagcgtccct ttttgtctgg gcggcttttt tcagctggtg
      721 ctcactatcg cggcgggaga ccgttgggcg gtctgggtgc ctgcgggcct gggagtgctt
      781 ggactggccc tgtcctttct cttcttgctt ggggcgattc catggggcgg tatcctgctc
      841 tactgggccc tgtattttct gtggatctgg cttatctggc tggtcgtggc gcagattaaa
      901 aagtcagttg gcagatggag agagaagcag gagagggccc gccgctgacc cggataaacg
      961 gggtttcctt tgacaggcgc ggtcttttca gacagtgccg tacatgtttt tggagaagga
     1021 agaaagtgag aggcgcttat gtatctgcac ctgggccagt cggtggcagt gccacatcgg
     1081 gagattttgg gcatctttga cctggataac gccagctggg cctataagac ccgggaattc
     1141 ctagagcggg cggaacagga gggccgagtc atctcggtgt gcgatgacct gccccgctcc
     1201 tttgtcctgg taggggagga gaaggggacg gccacagtct acatctctca gctgtcctcc
     1261 gccgcactgc tgcggcgggc ggagagcaat ttgctttgaa tcatgcagaa tgatggaatg
     1321 cagaatgcag agtgaagaag cccggtcctc cgggcggaac aagtggatat cggaacggca
     1381 gggaccagcg gagagagcct catctctccc gcttctgtat tctgccttct gcatttcgat
     1441 atggaggtta ttatggcaga agaattggaa ctgaactcca cccaggtgga gcacgagtac
     1501 ggcgcctcgg agatccaggt gctggaggga ctggaagcgg tgcgcaagcg gccgggcatg
     1561 tatatcggct ccacctcgga gtcaggtctg caccacctgg tctatgaaat cgtggacaac
     1621 tccattgacg aggccctggc gggctactgc accgacatca cggtgaccat caatcccggg
     1681 aacaccatca ctgtcactga caacggccgc ggcatccctg tggatatcca gccccaaacc
     1741 gggcgtcccg cgctggaggt ggtgttctct gtcctccatg ccggcggcaa gttcggcggt
     1801 gggggctaca aggtgtccgg cggcctccat ggcgtgggcg ccagcgtggt caacgccctg
     1861 tctgagtggc tggaggtcca ggtacacaag aacggccaga tctatgagat gaagttttcc
     1921 cggggaaaga tcacccagga gatgaaggtg gtgggaaaga ccgaccatac gggcacgacc
     1981 gtcaccttca agccagaccc ggagatgttc gagactctgg agtacaacta tgacactctc
     2041 cacacccgga tgagggagga ggccttcctc aatgctggcc tgcgcatcca gaccgtggac
     2101 ctgcgccccg gccgggagca ggaggacgat atgtgctacg aggggggcat ccgggaattc
     2161 gtcaccttca tcaaccgaag caaggacccg ctgcaccctg acgtgatcta tatgtccggc
     2221 aaccgggagg actccatggc tgaggtggcc ctccagtaca atgacagcta taacgaggtc
     2281 atcgtctcct tcgccaacaa cgtccatact ccggagggcg gcatgcatga ggagggcttc
     2341 aaacgtgccc tcaccaacgt gctcaacgcc tacggcaaga agatcggcgt g
//
LOCUS       DBMDSJ010000275         2387 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10407_length_2387_cov_1.726597, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000275 DBMDSJ010000000
VERSION     DBMDSJ010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10407_length_2387_cov_1.726597"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /gene="nadE"
                     /locus_tag="ACZPAQ_06960"
     CDS             <1..884
                     /gene="nadE"
                     /locus_tag="ACZPAQ_06960"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR0903763.1"
                     /translation="AVVGLSGGLDSTLAILITAVAMKLLDRPASDIIAVTMPCFGTTD
                     RTRDNAVELAERLGATLKRIDIGSAVKVHFKDIGQSMEDHSVTFENGQARERTQVLMD
                     IANQNGGLVIGTGDLSELALGWATYNGDHMSMYGVNAGIPKTLVRHLVAFVSDDKGSE
                     DPRLSAVLDDILDTPVSPELLPAIEGKISQKTEDLVGPYELHDFFLYYAIRWGFPPRK
                     VFRLAEHALGRTYDRATILKWLNSFYRRFFTQQFKRSCLPDGPKVGSVTLSPRGDWRM
                     PSDAVAALWLEELEGLE"
     gene            986..1594
                     /locus_tag="ACZPAQ_06965"
     CDS             986..1594
                     /locus_tag="ACZPAQ_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR0903764.1"
                     /translation="MNLYLDLFLTFAKVGVCTFGGGYAMLPILQREVVEKKGWVTEEE
                     VTDYFAIGQCTPGIIAVNTSVFIGYRQRGVPGGIAAALGVVFPSFVVITIIAAFLTKF
                     ATYPVVQHALAGINAAVVALIAVSVLKLGKTNLRGRPAVCIFLGVLVLGELSGLLAVD
                     PATPLGAVLDLLSNPAFLVVAAGATGWLLGRVKLPSQGGEDR"
     gene            1591..2157
                     /locus_tag="ACZPAQ_06970"
     CDS             1591..2157
                     /locus_tag="ACZPAQ_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR0903765.1"
                     /translation="MILLRLFYEFFKTGLFSVGGGMATIPFLQHMGETTGWFTNQDLT
                     TMIAVSESTPGPLGVNMATYVGYQTAGVPGALVATLGVITPGAILILIIAGFLQKFRS
                     NRSVEAVFAGLRPASTALIAAAGLSVAYSVFTASDALSPNSFPAIQWTAVVLTLAMFA
                     AMKWTPLKKLHPIAFIAAGAAAGILLQL"
     gene            complement(2202..>2387)
                     /locus_tag="ACZPAQ_06975"
     CDS             complement(2202..>2387)
                     /locus_tag="ACZPAQ_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903766.1"
                     /translation="SLTSGETFTLASGGQTEDGSYVYAALEAPAPGCDLFLISADKAR
                     TLSDLNYESLSANEEAE"
BASE COUNT          423 a          706 c          775 g          483 t
ORIGIN      
        1 cggcggtggt gggcctgtcc ggcgggctgg actccaccct ggccattctg atcaccgcag
       61 tggctatgaa gctgctggac cgcccggcca gcgatatcat cgcggtgacc atgccctgct
      121 tcggaaccac cgaccgtacc cgggataacg ccgtggagct ggccgagcgc ctgggggcca
      181 ccctgaagcg catcgacatc ggcagcgcgg taaaggtcca cttcaaggac atcggccagt
      241 ccatggagga ccactcggtg accttcgaga acggccaggc ccgggagcgg acccaggtgc
      301 tcatggacat cgccaatcag aacggcgggc tggtcatcgg caccggcgac ctgtccgagc
      361 tggccttggg ctgggccacc tacaacgggg atcacatgtc catgtatggg gtcaacgcag
      421 gcatcccaaa gactctggtg cgccatctgg tggccttcgt ctccgacgac aagggcagcg
      481 aggacccacg cctgtccgcc gtactggacg acatcctgga caccccagtc tccccggagc
      541 tgctgcccgc catcgagggg aagatcagtc aaaagacgga ggacctggtg ggcccctatg
      601 agctccacga cttcttcctg tactacgcca tccgctgggg cttccctccc agaaaggtgt
      661 tccgtctggc ggagcacgcc ttgggccgga cctacgaccg ggccaccatc ctgaagtggc
      721 tgaacagctt ctaccgccgg ttcttcactc agcagtttaa acgttcctgc ctgccagacg
      781 gccccaaggt gggctctgtc accctgtccc cccggggcga ctggcggatg cccagcgatg
      841 cggtggcggc gctgtggctg gaggaattgg agggcctgga gtaagagctc tcccgatccg
      901 ccggccgccg cggccggggc accgcggggg ccgggccggg cgggccgcac tttgctgaaa
      961 aatttatgga gaggacgttg atctgttgaa cctttatttg gacctgtttc tgacctttgc
     1021 caaggtggga gtatgtacct tcggcggtgg ctacgccatg ctgcccatcc tccagcggga
     1081 ggtggtggag aaaaagggct gggtcacaga ggaggaagtc actgactact ttgccatcgg
     1141 acagtgcact ccgggcatca tcgccgtcaa cacatcggtt tttatcggct accgtcagcg
     1201 gggggtccca ggaggaattg ccgccgccct gggagtggtg tttcccagct ttgtggtcat
     1261 caccatcata gccgcattcc tcacaaaatt tgcgacctat ccggtggttc agcacgccct
     1321 ggcgggcatc aatgccgcag tggtggcact gattgcagtc agcgtgctca agctgggaaa
     1381 gacaaatttg agggggagac ccgcagtatg catctttttg ggagttctgg tgctgggaga
     1441 gctgtccggg ctgctggctg tggacccggc caccccgctg ggagcggtgc tggatctcct
     1501 ctccaatcct gcattccttg tggtggcagc cggagcgaca ggctggctgc tgggccgggt
     1561 gaagctccca tcccaaggag gtgaggacag atgatcctgc tgcgcctctt ttatgagttt
     1621 tttaagactg gtctgttctc tgtgggcggg ggtatggcca cgattccctt tctgcagcat
     1681 atgggggaga ccaccggctg gttcaccaat caggatctga ctactatgat cgcggtttcc
     1741 gagtccacac cgggcccgct gggggtgaac atggccacct atgttggcta ccagaccgct
     1801 ggagtccccg gggcgcttgt ggcaacgctg ggggtgatca cgccgggggc catactgatc
     1861 ctgataattg cgggattttt gcagaagttt cggagcaacc ggagcgtgga ggcggtgttt
     1921 gccgggctgc gtcccgcctc cactgcgctg atcgcggcag cgggcctgag tgtggcgtat
     1981 tcagttttta cggcatcaga cgccctctca ccgaacagct ttccggccat tcagtggaca
     2041 gcggtggtgc tgacgctggc gatgttcgcg gcaatgaagt ggacgcccct gaaaaaactt
     2101 caccctatcg cctttattgc ggcaggagcg gcagccggca ttttacttca gctctaaacg
     2161 aaaaaagtgt cctcttccca tggcgggaag agggcacttt cttattccgc ctcttcattg
     2221 gcggacaggg actcatagtt cagatccgac agggtccggg ccttatcggc tgagatgagg
     2281 aagaggtcgc agccgggggc gggtgcctcc agggcggcgt agacgtagct gccatcctcg
     2341 gtctggccgc cgctggccag ggtgaaggtc tcgccggagg tcaggga
//
LOCUS       DBMDSJ010000276         2384 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10426_length_2384_cov_1.163382, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000276 DBMDSJ010000000
VERSION     DBMDSJ010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10426_length_2384_cov_1.163382"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="ACZPAQ_06980"
     CDS             complement(<1..423)
                     /locus_tag="ACZPAQ_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR0903767.1"
                     /translation="MNKYRVHEVAKDFKLNSKDIAEIMTKYSQTPKNHMQVLEERELA
                     VIFDYLTQHNQVENIESIFADVYHEPKPAQAKPAQPQQSASKPAAKGESRPQSAAPQA
                     DKSGEQPRAQGGKPEQAQARPATRVPEKKVVDTRKGGAV"
     gene            complement(445..1092)
                     /locus_tag="ACZPAQ_06985"
     CDS             complement(445..1092)
                     /locus_tag="ACZPAQ_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR0903768.1"
                     /translation="MPHDPILHLLGLAKKAGRLEIGEEPVGAAARAHQARVLLLAADA
                     APNTVRRAAHFGEAGGALWLQMPFTKAELGEQLGRSSCAMLAVTDFGFAAALAEKLAA
                     RDEERYGPAAQQLRAKADRALMRQREKRQHEKNMRQGKHKPWAAPPSRPSKREEAPPT
                     TSRRERGKGGSGRRPGRPETGTGRAPRTFPGKPGPGGRKLTAKPRSGGRNPAGKP"
     gene            complement(1082..1360)
                     /gene="rnpM"
                     /locus_tag="ACZPAQ_06990"
                     /gene_synonym="ylxR"
     CDS             complement(1082..1360)
                     /gene="rnpM"
                     /locus_tag="ACZPAQ_06990"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR0903769.1"
                     /translation="MPKKLPMRQCVGCREMRPKKELIRVVKSPEGAISLDFRGKLPGR
                     GAYLCPDSVCLARARKSKALERAFAQAIPPEVYERLEEQMKEVPGDAP"
     gene            complement(1455..>2384)
                     /gene="nusA"
                     /locus_tag="ACZPAQ_06995"
     CDS             complement(1455..>2384)
                     /gene="nusA"
                     /locus_tag="ACZPAQ_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR0903770.1"
                     /translation="SLEEARLSLPRVELGDTIRIEVKPKNFGRIAAQTARQVIIQGIR
                     EAEQGMIYDEFTSKEHEILTGIITRIDPRSGAVALRIGSGSESTEAYLSVNEQVRGET
                     YQEGSRLRVYVVEVRRATKGPQVLISRTHPGLVKRLFELEVPEIYDGTVEVKSIAREA
                     GSRTKLAVWSADPNVDPIGACVGPRGQRVNTIVEELKGEKMDIIKYSDDPAEYIAAAL
                     SPADVISVEPLPDGKSCRVVVPDDQLSLAIGKEGQNARLAAKLTGYKIDIKPASAPLE
                     PIEAPEEELFVEEAEAPAEDSAGTGETAPAEED"
BASE COUNT          314 a          813 c          768 g          489 t
ORIGIN      
        1 gacggctccg cccttgcggg tgtccaccac cttcttctcc ggcacgcggg tggcggggcg
       61 ggcctgggcc tgctccggct tgcccccctg agcgcggggc tgctccccgc tcttgtcggc
      121 ctggggcgca gcgctctgtg gcctgctctc ccccttggca gccggcttgg aggcgctctg
      181 ctgtggctgg gctggcttcg cctgggccgg cttgggctca tggtagacgt ccgcaaaaat
      241 ggactctatg ttctccacct ggttgtgctg ggtcagatag tcgaaaataa cggccagctc
      301 ccgctcctcc aggacctgca tgtggttctt gggggtctgg ctgtatttgg tcatgatctc
      361 cgcgatgtcc ttggaattca gcttgaagtc cttggccacc tcgtggacac ggtatttgtt
      421 catcatatag ggccacctcg tttcttaggg tttcccggca gggttcctgc cgccggatct
      481 gggcttcgca gtcagcttcc gcccgcccgg ccccggtttc ccggggaatg ttcggggcgc
      541 tctccccgtc ccggtctccg gcctgcccgg ccgtctgcct gagccgcctt tgccccgctc
      601 ccgcctggac gttgtcggcg gggcctcttc cctcttgctg gggcgagagg gcggggccgc
      661 ccagggcttg tgctttccct gtctcatatt tttctcgtgc tgacgttttt ctctctggcg
      721 catcagggcg cggtccgcct tagccctcag ctgctgagct gcggggccgt accgctcctc
      781 gtcccgagcg gccagcttct ccgccagggc ggcggcgaac ccgaagtcgg tcaccgcgag
      841 catggcgcag gagctgcgtc ccagctgctc ccccagctcc gccttggtga agggcatttg
      901 gagccacagg gcgcccccgg cctctccgaa gtgggcggcc cgccgcaccg tattgggggc
      961 ggcgtccgcc gccagcagga gcacgcgggc ctggtgggcc cgggcggccg cgcccaccgg
     1021 ttcctctccg atctccagcc gtccggcctt tttggccaaa cccaggagat ggaggatggg
     1081 gtcatggggc atcgccgggc acctccttca tctgctcctc caggcgctca tagacctctg
     1141 gagggatcgc ctgggcgaaa gcccgctcca gagccttgga ctttcttgcc cgggccagac
     1201 acacgctgtc cgggcacaga tacgccccgc ggccgggcag ctttccccgg aaatccaggg
     1261 agatggcccc ttccggggac ttgaccaccc ggatcagttc ttttttcggt ctcatttccc
     1321 ggcagccgac acattggcgc atagggagct tcttgggcac ggtccgcacc acccttcgac
     1381 gaattaggaa ttaggcgtaa ggaatgatgg ggagggggat gatccccagc tccccggtcc
     1441 cggttcttgt acggttagtc ttcctctgcg ggagcggtct ccccggtccc ggcagagtcc
     1501 tccgcaggcg cctcggcctc ctcgacgaac agttcctcct cgggagcctc gatgggctcc
     1561 aggggggccg aggccggctt gatatcgatc ttgtagcccg tcagcttggc tgccaggcgg
     1621 gcgttctggc cctccttacc gatggccagg gagagctgat catccggaac caccacccgg
     1681 cagctcttgc cgtcaggcag gggctccacg ctgatgacgt cggcaggaga gagggcggcg
     1741 gcgatgtact cggcggggtc gtcagagtac ttgatgatgt ccatcttttc gcccttcagc
     1801 tcttccacga tggtgttcac ccgctgtccc ctggggccga cacaggcacc gatggggtcc
     1861 acattggggt cagcggacca gacggccagc ttggtgcggg agcccgcctc ccgggcgatg
     1921 gacttgacct ccaccgtccc gtcatagatc tcaggcacct ccagctcaaa caggcgcttg
     1981 accagcccgg ggtgggtgcg ggagatgagc acctgaggac ccttggtggc ccggcgcacc
     2041 tccaccacat agaccctcag gcggctgccc tcctgatagg tctcgccccg cacctgctcg
     2101 ttgacgctga ggtaggcctc cgtgctctcg gagccgctgc cgatgcgcag ggccactgca
     2161 ccgctgcggg gatcaatccg ggtgatgatg ccagtgagga tctcgtgctc cttggaggtg
     2221 aactcgtcat agatcatccc ctgctccgcc tcccggattc cctgaatgat gacctggcgg
     2281 gcagtctggg cggcgatgcg gccgaagttc ttgggcttga cctcaatgcg gatggtgtcc
     2341 cccagctcca ctcggggcag ggacaggcgg gcctcctcca gact
//
LOCUS       DBMDSJ010000277         2379 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10452_length_2379_cov_1.776119, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000277 DBMDSJ010000000
VERSION     DBMDSJ010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10452_length_2379_cov_1.776119"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="ACZPAQ_07000"
     CDS             <1..1407
                     /locus_tag="ACZPAQ_07000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR0903771.1"
                     /translation="SRVKNGASLETAGLEEEIWQAYCARLEERGMLDFDDLLIRALAL
                     DTGGRTCFRHLLVDEFQDINETQYRLVRAWSASGSLFVIGDPDQSIYGFRGADGKCFQ
                     RLRAERPELREIRLVENYRSTPEVLEAAAPVIEKNPGGCRRLRPNRPSGPAVRMVRSP
                     DAFSEGVFLAKEIGRMTGGVDMLEAQALGHERTVRAFSDIAVLCRTHRQLELVEKCLR
                     HDDIPCVVSGREDFLEDRQVRGVLAFFRSLQSPADPAALETALGLLWDCPADLAGRAR
                     RLWTQGADLAALEEAVRGYGHLELWLDRVKEWEPAVEKEKPWKLVEGWEERYGTSPAL
                     EKLRNTAVFYPAFRSLWEALTLGEEADVRRAAGRGWESGAVRLMTLHASKGLEFPAVF
                     VAGLTEGTLPLEMPGRPADLEEERRLLYVGMTRAREELILSAGGGPSPFLDSLPQGVA
                     QEQAGRRQERRLEQLSLF"
     gene            1613..>2379
                     /locus_tag="ACZPAQ_07005"
     CDS             1613..>2379
                     /locus_tag="ACZPAQ_07005"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR0903772.1"
                     /translation="MKWNRLLAGAVCASLLSVPVWAVEGGEAPAAGAGQTSSTACGVT
                     LDGVCLDLSEAPAAPYQENGQVMLPLWKVAQALGWTVTWLPEENGTRIENEDQVMNLT
                     YGLDRYACASKSSIGATSPEHYGAAPVVVAGRTYVPASMFQLLSCTVTMEGGTVALRT
                     EGAPPAEDEPPSGGAVEYQTAAEAEQAVGFPLKAPGELGAPEKIQVISGAVAQLNWPE
                     GISYRVARGSQEVSGSYTAYGWERSQSAGEQMVTIKG"
BASE COUNT          417 a          688 c          898 g          376 t
ORIGIN      
        1 tcccgggtga aaaacggcgc gtctctggag acggctggac tggaagagga gatctggcag
       61 gcctactgtg cccggctgga ggagcggggg atgctggact ttgatgacct gctcatccgg
      121 gcgctggcgc tggacactgg gggccggacc tgcttccggc acctgctggt agacgagttc
      181 caggacatca acgagaccca gtaccggctg gtccgggcct ggagcgccag cgggtcgctc
      241 tttgtcatcg gagacccgga ccagtctatc tacggtttcc ggggggcgga cgggaagtgt
      301 ttccagcgcc tgcgggcgga acggccggag ctgcgggaga tccggctggt ggagaactac
      361 cgctccacgc cggaggtgct ggaggctgcc gcccccgtga tcgagaaaaa tccgggcggt
      421 tgccgccgcc tgcgccccaa ccggccctcc ggcccggcgg tacggatggt gaggagcccg
      481 gatgccttct ctgagggggt gtttctcgcc aaggagatcg gccgtatgac cggcggggtg
      541 gacatgctgg aggcccaggc cctgggccac gagcggacag tgcgggcttt ctctgacatc
      601 gccgtcctct gccgcaccca tcggcagctg gagctggtgg agaagtgcct gcgtcacgac
      661 gacatcccct gtgtggtcag cgggcgggag gactttctgg aggaccgcca ggtgcgggga
      721 gtactggctt tcttccgctc cctccaaagc ccggcggacc ctgcagccct ggagaccgcg
      781 ctgggccttc tgtgggactg tcccgccgac ctggccggaa gggcccgccg cctctggaca
      841 cagggggcag acttggcggc cttggaggag gcggtccgag gctatggcca tctagagctt
      901 tggctggacc gggtgaagga gtgggagcca gcggtggaga aggagaagcc atggaaactg
      961 gtagagggct gggaggagcg gtacgggacc tcgccggcct tggagaagct gcggaatacg
     1021 gcggtgttct acccagcctt ccgctccctc tgggaggccc tcactctggg ggaggaggcg
     1081 gatgtgcgcc gggctgcggg ccggggctgg gagtccgggg ctgtccggct gatgactctc
     1141 cacgcctcca agggactgga gtttcctgcc gtctttgtgg cgggactgac agaaggcacg
     1201 cttccccttg agatgcccgg ccgtccggcg gatctggagg aggagcgccg gctgctctat
     1261 gtggggatga cccgggcccg ggaggagctg atcctcagcg ccgggggcgg accctctccc
     1321 ttcctggaca gcctgccgca gggtgtggcc caagagcagg cgggccggag gcaggagcgg
     1381 agactagagc agctgagcct cttctgagac ggggagcccc caggtccggc agaatcagta
     1441 cactggaaaa gagagacatc tgcggctgcg cccttgacag tgggtgtgtg tcccattagt
     1501 atggaggagc ggggacgaaa aaataaacgg tccgggatgg aacaaaatcc cgtgtcgggt
     1561 cgtctataca aatagaagga acactttgga taccaggaaa ggaaacgcga ctatgaaatg
     1621 gaatcggctg ttggccggcg cggtgtgcgc ctccctgctg agcgtacccg tctgggctgt
     1681 ggagggcggg gaggctccgg ccgccggggc gggccagaca tcttccacgg cctgcggggt
     1741 gacgctggac ggagtttgcc tggacctgag cgaggcgccc gccgcgccct accaggagaa
     1801 cggacaggtg atgctcccgc tgtggaaggt ggcccaggcc ctgggatgga ctgtgacctg
     1861 gctgccggaa gagaacggga cccgcatcga aaatgaggat caggtcatga acctgaccta
     1921 cggattggac cgctatgcct gcgccagcaa gtcctccatc ggggctacct cgccggagca
     1981 ctatggagcg gcgccggtgg tggtggcggg acgtacctac gtccccgcct ccatgttcca
     2041 gctgctctcc tgcaccgtga cgatggaggg ggggaccgtg gccctgcgga cagagggcgc
     2101 accacccgcg gaggacgaac cgccctccgg cggggcagtg gagtatcaga ccgcagccga
     2161 ggcggagcag gcggtggggt tcccattgaa agctcccggg gagctgggag cccctgaaaa
     2221 gatccaggtg atcagcggag cggtggccca gctgaactgg ccggagggca tcagctatcg
     2281 ggtggcccgg gggagccagg aggtcagcgg cagctacact gcctacggct gggaacggag
     2341 ccagagtgcg ggagaacaga tggtgaccat caaggggaa
//
LOCUS       DBMDSJ010000278         2367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10522_length_2367_cov_1.386143, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000278 DBMDSJ010000000
VERSION     DBMDSJ010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10522_length_2367_cov_1.386143"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="ACZPAQ_07010"
     CDS             complement(<1..142)
                     /locus_tag="ACZPAQ_07010"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR0903773.1"
                     /translation="MERNYTTQMDAARRGIVTPELETVARKEHMSVEELMPLVAAGKV
                     AIP"
     gene            complement(208..1041)
                     /gene="thiD"
                     /locus_tag="ACZPAQ_07015"
     CDS             complement(208..1041)
                     /gene="thiD"
                     /locus_tag="ACZPAQ_07015"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR0903774.1"
                     /translation="MKTALTIAGSDSSGGAGIQADIKTMTANGVYAMSAVTALTAQNT
                     TGVYGILESTPEFLASQLDCIFTDIFPDAVKTGMVSSTGLIQVIADKLKQYQARNIVV
                     DPVMIATSGSRLISEEAVEALKSQLLPLAAVLTPNIPEAEVLSGLTISGPEDMERAAR
                     EIGERYGCAVLCKGGHDLNDANDLLWQDGSCKWFCGRRIHNPNTHGTGCTLSSAIASN
                     LAKGCDLETAVERAKIYLSGALSSMLDLGAGSGPLDHLFSIPELDLDRIRSLQSPAGG
                     R"
     gene            complement(1330..1989)
                     /locus_tag="ACZPAQ_07020"
     CDS             complement(1330..1989)
                     /locus_tag="ACZPAQ_07020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0903775.1"
                     /translation="MRIEGAIFDLDGTLTDSMYIWNEAPKALVRKFGGKPPEDLARDI
                     REMGRREASEYMVDTFCLPCTPEQVMEGVNSLVTGEYRDRVPMKTGADTLLERLGALG
                     VPCGIATASEAFQARDAMVRLGLWKHFLFAFSSLQYGPKTGPDLYRAAARSLGSAPER
                     TVVFEDALHAARSAKRAGFLVAGVYDPSAEEDQEALRCLCDWYLPRLDSPDFLAALER
                     G"
     gene            complement(1986..>2367)
                     /locus_tag="ACZPAQ_07025"
     CDS             complement(1986..>2367)
                     /locus_tag="ACZPAQ_07025"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR0903776.1"
                     /translation="VGQSDMEAGRVRALLGEDKLIGVSARTVEQALAAQAAGADYLGV
                     GAMFPTGTKQDTRPVSYDTLKAICAAVDIPVVAIGGIGAGNVAELAGSGIAGVAVVSA
                     LYAQPDVEAATRSLRAQVERVVGP"
BASE COUNT          363 a          840 c          724 g          440 t
ORIGIN      
        1 cagggatggc caccttgccg gccgcgacga ggggcatcag ctcctccacg gacatgtgct
       61 cctttcgggc gacagtctcc agctccgggg tcacgatccc ccggcgggcg gcgtccatct
      121 gtgtggtata attgcgttcc ataaagatct tctcctttat ctattgattc ctatttcatg
      181 gatctcggtc atcaggcgga ccgcccctca ccttcctccg gcgggggact gaagggaccg
      241 gatgcggtcc aggtccagct ccgggatgga aaagaggtgg tccagggggc cgctgcccgc
      301 ccccaggtca agcatggagg acagcgcccc cgagagatag atcttcgccc gctccaccgc
      361 cgtctccagg tcgcagccct tcgccagatt ggaggcgatg gcactggaga gcgtacagcc
      421 tgtcccgtgg gtgttggggt tatggatccg gcgtccgcag aaccatttgc agctcccatc
      481 ctgccacagc aggtcgttgg cgtcgttcag gtcgtgcccg cccttgcaca gcacagcgca
      541 gccgtaccgc tccccgatct cccgggcagc ccgctccatg tcctccggac cggagatggt
      601 gaggcccgac aggacctccg cctctgggat gttgggggtg aggacggcgg ccagggggag
      661 cagctggctc ttcagcgcct ccaccgcttc ctccgaaatc aggcgggacc cgctggtagc
      721 gatcatcacc gggtccacca cgatgttccg ggcctggtac tgcttcagct tgtccgcgat
      781 gacctggatc agcccggtag aggacaccat ccctgtcttg acggcatcgg ggaagatgtc
      841 ggtaaaaatg cagtccagct gactggccag gaattccggg gtggactcca ggatgccgta
      901 gacgccggtg gtattctggg ccgtcagggc ggtgaccgca ctcatggcgt agacgccgtt
      961 ggcagtcatg gtcttgatgt cggcctggat cccggcgcct ccgctggaat cgctcccagc
     1021 gatcgtcaat gctgttttca tcagaaaact cctttctccg cattgaagtt ttgttccgcg
     1081 acaccaggtg gtgcccccga ggtgccgcgt cgtcggagca aagtacgctc tgttcgggac
     1141 agcctgacgg ccatccctca ctgcgctccc ttcctcctct ccgggccgca cccgctccgc
     1201 tgggctgcgc cccggctggg cggccttacg gctgccccca tttttgctgt gttcgggacg
     1261 gtctgacggc catccctcac tgtgctcctc tgcccctcct ctccgggccg cacccgctcc
     1321 attcccggct cagccccgct ccaatgctgc caggaaatcc ggggagtcca gccggggaag
     1381 ataccagtcg cagaggcacc gcagtgcctc ctgatcctcc tcggcggagg ggtcatacac
     1441 ccccgccacc agaaagcccg cccgtttggc gctgcgtgcg gcatggaggg catcctcaaa
     1501 gaccaccgtg cgctccggcg cgctgcccag gctgcgggca gcagcccgat agaggtccgg
     1561 tccggtctta ggcccgtact gaagggaact gaaggcgaac aggaagtgct tccacagccc
     1621 cagacggacc atggcgtccc gtgcctgaaa ggcctcgctg gcggtggcga tgccgcaggg
     1681 gacccccagg gctcccagcc gctccagcag tgtgtccgcc cccgtcttca tggggaccct
     1741 gtcccggtat tcccctgtca ccagggagtt caccccttcc atcacctgtt cgggcgtaca
     1801 ggggaggcaa aatgtatcca ccatgtactc cgaggcttcc cgccgcccca tctcccggat
     1861 gtcccgggcc aggtcctccg gcggctttcc tccaaacttc cggaccagag ccttgggcgc
     1921 ctcgttccag atgtacatgg agtcggtgag ggtcccgtcc agatcgaaga tggccccctc
     1981 gatcctcatg ggcccaccac ccgctccacc tgggcccgga gggagcgggt ggccgcttcc
     2041 acgtcgggct gggcgtacag ggctgagacc accgccaccc cggcgatgcc gctccccgcc
     2101 agctccgcca cattgccggc tccgatgccc ccgatggcca ccacagggat atccacggcg
     2161 gcgcagatgg ccttcagggt gtcatagctc actggtcggg tgtcctgctt ggtcccggtg
     2221 gggaacatgg ctcctacccc caggtagtcg gcccccgccg cctgggcggc cagggcctgc
     2281 tccaccgtgc gtgcggacac gccgatgagt ttgtcttccc ccagcagggc ccggactcgc
     2341 cccgcctcca tatcgctctg gcccacg
//
LOCUS       DBMDSJ010000279         2360 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10559_length_2360_cov_2.065073, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000279 DBMDSJ010000000
VERSION     DBMDSJ010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10559_length_2360_cov_2.065073"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="ACZPAQ_07030"
     CDS             <1..885
                     /locus_tag="ACZPAQ_07030"
                     /inference="COORDINATES: protein motif:HMM:NF015509.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR0903777.1"
                     /translation="YGVGKLKLITGEQSRVLSCMCIYLAVPCSLMTSFNTAMEADKLT
                     GLLLALGAAVLLHLLYLALNGLMNRSGHGLAPEEQASVIYNNAGNLILPMVMAILGQE
                     YVIYTSVYILVQNILMWTHGQKLMGGAQQFDWKKIVTTPAIAAILAGLALFITGLRLP
                     GPLVTAMEGLGSCMAPLSMLVIGILFSELDLKRVLCQGRIYWVAGLRLLVYPLLSMAV
                     LLALRVLWPHSDGDNVLLVSLLCAIGPAASAITQMAQLYHNPNSGYVSSINVLTTLLC
                     AVTMPVMVLLFQLGLDLL"
     gene            complement(975..1634)
                     /locus_tag="ACZPAQ_07035"
     CDS             complement(975..1634)
                     /locus_tag="ACZPAQ_07035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0903778.1"
                     /translation="MKIEGVVFDVDGVLFDTERLTQQTWLAVSREMGWPQVGEAYLEF
                     VGQNRADIFQKMEDLFGAEFPKDAFMAACSARSQARMETEGVPMKPGVGEVLELLRSR
                     GVPTALATSTGRPRTERRMEMTGLGPCFSAIITGDQVAHSKPDPEIYLMACRALGTTP
                     ERTVAVEDSRNGILSASRAGMRVAMVPDLLPPTPELEPLLWGRFDSLLQLRDRLAKEL
                     D"
     gene            complement(1640..>2360)
                     /gene="purD"
                     /locus_tag="ACZPAQ_07040"
     CDS             complement(1640..>2360)
                     /gene="purD"
                     /locus_tag="ACZPAQ_07040"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR0903779.1"
                     /translation="PTVVIEECMVGPEVTVLAFCDGKHLVPMLSSQDHKRAYDGNQGP
                     NTGGMGAFCPSPKYTPEIAERCMKEIFLPTVAAMKAEGRPFKGVIYFGLMLTQDGPKV
                     VEYNARFGDPETQPLMSMLDTDLMDIFQACVDGTLDQMDIRWKPGASCCIVLASGGYP
                     VKYQSGYPISGLEAAGKLATVFHAGTKLGEDGTVLTAGGRVLGVTAVGGDLEEAIEKA
                     YAACKPISFQDMHFRTDIGKV"
BASE COUNT          397 a          745 c          768 g          450 t
ORIGIN      
        1 tatggtgtgg gaaaactgaa gctgatcaca ggggagcaga gccgggtgct ctcctgcatg
       61 tgtatctatc tggcggtgcc gtgcagcctg atgacctcct tcaacacggc catggaggcg
      121 gacaagctga ccggcctgct gctggccctg ggggcggcag tgctgctcca cctgctctac
      181 ctggcgctca atggcttgat gaaccggagc gggcacggac ttgccccgga ggagcaggcg
      241 tccgtgatct acaacaatgc aggcaacctg atcctgccca tggtaatggc cattctgggg
      301 caggagtatg tgatctacac cagcgtctat atcctggtgc agaacatcct gatgtggacc
      361 catggccaga agctgatggg gggtgcccag cagtttgatt ggaaaaagat cgtgaccaca
      421 cccgccatcg ccgccatcct ggcgggactg gcgctgttca tcaccgggct gcgcctgccg
      481 gggcctctgg tcaccgccat ggaggggctg ggcagctgca tggcacccct gagcatgctg
      541 gtgatcggga tcttgttttc tgagctggac ctgaagcggg tgctctgtca ggggcgcatc
      601 tactgggtgg cggggctgcg tctgctggtc taccccctgc tctccatggc ggtgctcctg
      661 gccctgcggg tgctgtggcc ccacagcgac ggggacaacg tgctgctggt cagccttctg
      721 tgcgccattg ggccggcggc ctccgccatc actcagatgg cccagctcta ccacaacccc
      781 aacagtgggt atgtctcttc catcaatgtg ctcaccaccc tgctgtgcgc ggtgaccatg
      841 ccggtaatgg tactgctgtt tcagctggga ctggacctgc tgtgagcaga gggccggggc
      901 ttttccggag acctgagatc agaaaaaagc gcctggacct ccttatctca gaggtccagg
      961 cgtatctttt tggttcagtc cagctccttt gccagacggt cccgcagctg gagcagggag
     1021 tcgaagcgcc cccagaggag gggctccagc tcaggagtgg ggggcagcag gtcggggacc
     1081 atggccaccc gcatccccgc ccgggaggcg gagaggatgc cgtttcggga gtcctccacc
     1141 gccacagtgc gctccggggt ggtccccagg gccctgcagg ccataaggta gatctccggg
     1201 tcaggcttgc tgtgcgccac ctgatccccg gtgatgatgg cggaaaaaca gggacccagg
     1261 ccggtcatct ccatccgccg ctctgtccgg gggcggccgg tggaggtggc cagggcggtg
     1321 gggacgcccc ggctccgcag cagctccagc acctccccca cgccgggctt catagggacg
     1381 ccctccgtct ccatccgggc ctgggagcgg gcggaacagg ccgccataaa tgcgtccttg
     1441 ggaaactccg ccccaaagag atcttccatt ttctgaaaaa tgtcggcacg gttctggccc
     1501 acaaactcca gataggcttc tcccacctgg ggccagccca tttcccggct gacggccagc
     1561 caggtctgct gggtgagacg ctcggtgtcg aacagcaccc cgtctacatc aaagacgacg
     1621 ccttctatct tcaagccggt cataccttcc caatgtccgt gcggaagtgc atgtcctgga
     1681 aggagatggg tttgcaggcg gcgtaggcct tttcgatggc ttcctccagg tctccgccca
     1741 cggcagtcac tcccagcacc cggccgccgg cggtgaggac ggtcccgtcc tcacccagct
     1801 tggtgccggc gtggaacacc gtggccagct ttccggctgc ctccagaccg gagatggggt
     1861 agccgctctg gtacttcacc gggtagccgc ctgaggccag cacgatacag caggaggcgc
     1921 cgggcttcca gcggatgtcc atctggtcca gggtgccgtc cacacaggct tggaaaatgt
     1981 ccatcaggtc ggtgtccagc atggacatga ggggctgggt ctccgggtcg ccgaagcggg
     2041 cgttgtactc caccaccttg gggccgtcct gagtgagcat caggccgaag tagatgaccc
     2101 ccttgaaggg cctgccctcc gccttcatgg cggccactgt gggcaggaag atctccttca
     2161 tgcagcgctc ggcgatctcc ggggtgtact tgggggaggg gcagaaggcg cccatgccgc
     2221 cggtgttggg accctgattg ccgtcgtagg cccgcttgtg gtcctggctg gacagcatgg
     2281 ggaccaggtg cttcccgtcg cagaaggcca gcacagtcac ctcgggaccc accatgcact
     2341 cctcgatgac cacggtgggt
//
LOCUS       DBMDSJ010000280         2346 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10614_length_2346_cov_1.582183, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000280 DBMDSJ010000000
VERSION     DBMDSJ010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_2346_cov_1.582183"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="ACZPAQ_07045"
     CDS             <1..514
                     /locus_tag="ACZPAQ_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HIR0903780.1"
                     /translation="ELNVQGGALDGVDTWLAELRRESTLAEAESWGLERIIALLARRP
                     VADTPTGMRKALAALMRIGGDSFTLEAINATISGCGVHAHVEEKEIPGEVVVSFPDIP
                     GIPKGFKEIREIIEDILPAHLGIEYVFWYITWEELERKISCWAEIEERQLTWEQLETL
                     VDETEEWAGT"
     gene            complement(542..934)
                     /locus_tag="ACZPAQ_07050"
     CDS             complement(542..934)
                     /locus_tag="ACZPAQ_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR0903781.1"
                     /translation="MDYVEKARALRADPAVHYNCCQAVLVTFADRLGLTPEQANALGA
                     HFGSGMRHGSTCGAVSGALMVLGLLGCDEKQSTALLRDFRTRHGALDCADLLRASKER
                     GELRKDHCDSLVLEMSRFLEELLREQST"
     gene            complement(976..1419)
                     /locus_tag="ACZPAQ_07055"
     CDS             complement(976..1419)
                     /locus_tag="ACZPAQ_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR0903782.1"
                     /translation="MLTFRDMTLDDRDTVLPMVDAFYRSNAVDHPVPREIVERSFLAA
                     AGNEPLLRGTLIFEEGRLAGYLYVTLCYSAEVGGTCVFIEEVYLLPEFRGRGLGRQVL
                     EWIFTQYPDSRRFRLEVTQMNQGAAHLYEKCGFQYLRYDQMVLDR"
     gene            1538..2011
                     /locus_tag="ACZPAQ_07060"
     CDS             1538..2011
                     /locus_tag="ACZPAQ_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR0903783.1"
                     /translation="MTEQEFALRAQDQRGRLYRTAFLYLGGEHAAVDAVDEAVYRAFR
                     DRRKLRQPEFFETWLTRILINVCKDELRRRRRECRVCVPPEPEAEHMDALSLREAVRA
                     LPEELRSVIVLRYFTGLTLEDTVPLEIPRGTVSSRQRRALELLRLDLTDGQEVGS"
     gene            2008..>2346
                     /locus_tag="ACZPAQ_07065"
     CDS             2008..>2346
                     /locus_tag="ACZPAQ_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HIR0903784.1"
                     /translation="MNRREEYWVLTEQLRREPPELESAVRRGMDRARRRRRRPAELLA
                     GLAGVCAAFVVMVNASPAFAISCAKVPVLRELAAAVAFSPSLRAAVEHDYVQYIGRTE
                     GADRISVTAEY"
BASE COUNT          437 a          709 c          807 g          393 t
ORIGIN      
        1 ggagctgaat gtccagggcg gggcgctgga cggggtggac acctggctgg cggagctgcg
       61 gcgggagagc acgctggcgg aggcggagag ctgggggctg gagcggatca tcgccctgct
      121 ggcccggcgg ccggtagcgg acacgccgac ggggatgcgg aaagccctgg ctgcgctgat
      181 gcggatcggc ggggacagct tcaccctgga ggccatcaac gccaccatct ccgggtgcgg
      241 cgtccatgcc catgtggagg agaaggagat cccgggagag gtggtggtct ccttccctga
      301 cattccaggc atcccaaagg gatttaagga gatccgggag atcattgagg atatcctgcc
      361 agcccacctg gggatcgaat atgtcttctg gtatattaca tgggaggaac tggagagaaa
      421 gatctcctgc tgggcggaga tcgaagagcg ccagctgacc tgggagcagc tggagacact
      481 ggtggatgaa acagaggagt gggcggggac ctgacagtcc ccgcccactc ctctgtctgg
      541 gtcaggtgct ctgctccctg agcagttcct ccagaaatcg agacatctcc aggaccaagc
      601 tgtcgcagtg gtccttgcgc agctctcccc gttccttgga agcccggagc aggtcggcgc
      661 agtccagcgc gccgtgacgt gtccggaagt ctcggagcag tgcggtggac tgcttctcat
      721 cgcagcccag caggcccagc accatcagag cgccgctcac cgcgccgcag gtggagccgt
      781 gacgcatccc ggacccaaag tgggccccca gagcattggc ctgttccggc gtgagtccca
      841 gccggtcggc aaaggtcacc aggaccgcct gacaacagtt gtagtggacc gcagggtctg
      901 cccggagtgc ccgggccttt tcaacatagt ccatttcaag ttttcctccc acaataaatc
      961 acgatggcct gctggtcacc ggtccagcac catctggtcg tagcgcaggt actggaagcc
     1021 gcacttctca tacagatggg cggccccctg gttcatctgg gtgacctcca gacggaagcg
     1081 gcggctgtcc gggtactggg taaagatcca ctccaggacc tgccggccta ggccgcggcc
     1141 acggaactcc ggcaggagat agacctcctc aatgaagacg caggtcccgc ccacctccgc
     1201 ggagtaacac agggtgacat acagatagcc cgccagccgg ccttcctcaa agatcagggt
     1261 gccccggagc aggggctcat tcccggcagc agccagaaag gaccgctcca cgatctcccg
     1321 ggggaccggg tggtctaccg cgttggaccg gtagaaggca tccaccatgg gcaggacggt
     1381 gtcccggtcg tccagtgtca tatcgcgaaa ggtcagcata gcgatcctcc tttgtttcat
     1441 tgtctgctat tataatatca cagaaaaatt ttttcaaccg gaggtgggca aaacatggac
     1501 ctccggcgtc taatggtcaa aaagggaggg atggagcatg acggaacagg aattcgccct
     1561 ccgggcccag gaccagcggg gtcggctcta ccgcacagcc ttcctctacc taggcgggga
     1621 gcacgctgcg gtggacgcgg tggacgaggc ggtgtaccgc gccttccggg accgccggaa
     1681 gctgcggcag cccgagttct tcgagacctg gctgacccgg atcctcatca atgtgtgcaa
     1741 ggacgagctg cgccgccgcc gcagagagtg ccgggtctgc gtcccgccgg aaccggaggc
     1801 ggagcacatg gatgccctct ccctgcggga ggcggtgcgg gcgctgccgg aagagctgcg
     1861 atccgtgatc gtcctgcggt actttaccgg cctgacgctg gaggataccg tccccctgga
     1921 gatccccagg gggacggtat cctcccgcca gcggcgggcc ctggagcttc tcaggctgga
     1981 cctgacagac ggacaggagg tggggtcatg aaccggagag aggaatactg ggtgctgacg
     2041 gaacaactgc gccgggagcc cccggaactg gagagcgcgg tccggcgagg gatggaccgc
     2101 gccaggcgga ggcgccggcg gccagcggag ctgctggccg gactggctgg tgtgtgcgcc
     2161 gctttcgtgg tgatggtgaa cgcctccccg gcctttgcca tctcctgcgc caaggtgccg
     2221 gtcctgcggg agctggccgc ggcggtggcc ttctctccca gcctgcgggc ggcggtggag
     2281 cacgactatg tgcagtatat cggccggaca gagggggcgg acaggatctc cgttactgcg
     2341 gagtat
//
LOCUS       DBMDSJ010000281         2341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10645_length_2341_cov_1.556250, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000281 DBMDSJ010000000
VERSION     DBMDSJ010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10645_length_2341_cov_1.556250"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(261..1175)
                     /locus_tag="ACZPAQ_07070"
     CDS             complement(261..1175)
                     /locus_tag="ACZPAQ_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR0903785.1"
                     /translation="MGSEAMSPHVMKKLAKPMLFAAAIIWGTSFFVMKNALDVIPVFY
                     LLAIRFTAGAVLLAAVCWKKWAGFTWDYLWRGAVIGAFLFLAYSVQTFGLDRTTPSKN
                     AFLTAVYCVIVPFLYWAAARRRPDRYNILAALLCVTGVGLVSLDGDLSVNAGDVLTLI
                     CAFFYAAHIVAVAKVSPEKDIYLLTVFQFAFAALYAWAGGALFENFPAQAFLAPAVCL
                     PMAYLCVMATTVALLFQNVGQVWSDPASASVILSLESVFGVISSVLFYGDLVTLPMAV
                     GFACIFVAVVCSETKFSFLTAGRQKERR"
     gene            1334..1984
                     /locus_tag="ACZPAQ_07075"
     CDS             1334..1984
                     /locus_tag="ACZPAQ_07075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0903786.1"
                     /translation="MYFFDLDGTLLDSNGIWLDIDIEFLGRRGISPVPEDYTDYVTHH
                     SAPDAARYTRERFSLPETPEEIMEGWMSMARSAYAHTLPLKPGVPAFLDACRRRGTRM
                     AVLTSCMPPLCRAALEHHRLLDCFEAVYTTAELGIEKRDPALYCLAAERSGVPPQSCL
                     LFEDSPSYCAAARQAGFTVVGVFDPLFAPRWGEMEALCHHRLERFADASPTLWQIP"
BASE COUNT          454 a          763 c          659 g          465 t
ORIGIN      
        1 gcccactcat ccacggtctc gcctgtaaat accgacgccg cggagactgg cgcggccgtc
       61 aaagcggccg ccagggcccc gctcagcagt gcggacaacc attttctttt catcaggtcc
      121 acttccttct tcttgtcgcg cgccccgccg gggcggcccg ttcccttctc cctcttgtcc
      181 tcgatttgat tctacacgct ttttttcaaa agcgcaatcc tcttttctcg tctgaaaatg
      241 ttggaagccc tttccgcccg tcagcggcgt tctttctgcc tcccggcggt caggaaagag
      301 aacttcgtct cagagcacac caccgccaca aagatgcagg caaagcccac cgccatgggg
      361 agagtgacca gatccccgta gaagaggacc gagctgatga cgccgaacac cgattccagg
      421 gagaggatca ccgacgcgga ggccgggtcc gaccacacct gccccacgtt ttggaagagc
      481 agggccacag tggtggccat aacgcacagg taggccatag gcaggcagac cgccggggcc
      541 aggaacgcct gagcggggaa gttctcgaac agcgctcccc ctgcccaggc atacagcgcc
      601 gcaaaggcaa actggaatac ggtgagcagg tagatgtcct tctccgggga cactttggcc
      661 accgccacga tgtgggcagc gtaaaagaaa gcgcagatca gggtgagcac gtcccctgcg
      721 ttgacagaca ggtccccgtc cagagagacc agtcccactc cggtgacaca gagcagggcg
      781 gccaggatgt tgtaccggtc cgggcgccgc cgggcggctg cccagtagag aaaggggacg
      841 atcacgcagt acaccgccgt caaaaatgca tttttggagg gtgtggtccg gtccagtcca
      901 aaagtctgga ccgaataggc caggaacagg aaagccccta tcaccgcccc acgccacaga
      961 tagtcccagg taaagcccgc ccacttcttc cagcagaccg ccgccagcag gaccgcgccc
     1021 gcagtaaagc ggattgccag caggtagaac accgggatca cgtccagggc gttcttcatt
     1081 acaaagaagg aggttcccca gataatggct gccgcgaaca gcatgggctt ggccagtttt
     1141 ttcatcacat gcggagacat ggcttcactt cccatcttga actagtctcc cccggcgtgg
     1201 tatgatagga aagaagcatg ccgggccggt ctgtcaaaaa cggagctatt ataccagaaa
     1261 accgtcccgg ccgcaacccg ggggctggcc ccgccccagg caggaggccc ccatcctgca
     1321 cggaggtccc gccatgtact tttttgatct ggacggcacc ctgctggact ccaacgggat
     1381 ctggctggac atcgacattg aatttctggg ccggcggggc atctcgccgg tgcctgagga
     1441 ctacaccgac tacgtcaccc accacagcgc tcccgacgcg gcccggtaca ccagggagcg
     1501 cttctccctc ccggagacgc cggaggagat catggagggc tggatgtcca tggcccgctc
     1561 cgcctatgct cacactctgc ccctgaagcc cggcgtgccc gcctttctgg acgcctgccg
     1621 ccgccggggg acacggatgg cggtgctcac ctcctgcatg cctcccctgt gccgcgccgc
     1681 cctggagcac caccgccttc tggactgctt tgaggccgtc tacaccaccg ctgagctggg
     1741 aatagaaaag cgggacccgg ctctctactg tctggcggcg gaacggtcgg gcgtccctcc
     1801 ccagtcctgc ctcctcttcg aagattctcc cagctactgt gctgcggcca gacaggctgg
     1861 cttcactgtg gtaggggtct ttgaccccct ttttgccccc cgctgggggg agatggaggc
     1921 cctgtgccac catcgcctgg agcgatttgc ggatgcctct cccaccctct ggcagattcc
     1981 ataattttgc tctttcctgc ccctgaaaat gtgttaatgt tttgttaaga tgtttcttga
     2041 cttttgcccg attccgcact atatatatag ttcggaatcg ggcactttct gttttagaca
     2101 aagaaagacc cttatttttg tgaaaatgcc cgaggccgct ccctctttct gatcacatct
     2161 gctccggtct ttcggagcct gaggaggccc ctctcatgat ccaaagaaat ttctggcgca
     2221 atgtgctccc ctccgtcatc gcctttgcct tctccggcgt ctactccatc gtggacggga
     2281 tgttcgtggg ccagggcgtg ggcgatgccg ggctggccgc catcaatgtg gcctggccgg
     2341 t
//
LOCUS       DBMDSJ010000282         2324 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10737_length_2324_cov_1.527665, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000282 DBMDSJ010000000
VERSION     DBMDSJ010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10737_length_2324_cov_1.527665"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="ACZPAQ_07080"
     CDS             <1..716
                     /locus_tag="ACZPAQ_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR0903787.1"
                     /translation="RGALFGRNSDFLTELEEHNANCLYRFSDGGYSFVGNTTSFLQME
                     DGVNQFGLAAGLTSVPPNPPRPGLNAGLTLRLLLESCRDVPQALALLRQLPVSSCHTL
                     VLADRAGRIALAECAPERTQIHCPGETDAFVCSVNSFHLSAMAPFRRQTDDDWRAEER
                     YLTLTRSLAAWGPELDLQAGQDLLSGRMGFLCQYDRREGRDTVWSVLYDTASGTLLRA
                     EGNPGRLPFQPDSRLSFRS"
     gene            864..1175
                     /locus_tag="ACZPAQ_07085"
     CDS             864..1175
                     /locus_tag="ACZPAQ_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039453.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6442 family protein"
                     /protein_id="HIR0903788.1"
                     /translation="MMDKEDILKRSRREKDEGLTYAQDRGRQYGVVGFLVLFLVIMIY
                     NMVRGLDNSLPLTLFWAYLSCEAAGRYFARKEKSVLATAVLAGLASLFSLVSYILETL
                     P"
     gene            1172..1303
                     /locus_tag="ACZPAQ_07090"
     CDS             1172..1303
                     /locus_tag="ACZPAQ_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903789.1"
                     /translation="MSPARPLGPARLVLTFVLLSALFLLALLAGMLRLLVLALVPAQ"
     gene            1325..1639
                     /locus_tag="ACZPAQ_07095"
     CDS             1325..1639
                     /locus_tag="ACZPAQ_07095"
                     /inference="COORDINATES: protein motif:HMM:NF039453.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6442 family protein"
                     /protein_id="HIR0903790.1"
                     /translation="MNKEEILAKSRKENVLQDEMERTVRIEGESFSLILVFLVGVVIV
                     IFNRLHGLPANSVLAMFWASCVGNRLYRLTKRRNTSDVVTLLISLAFLAWNLVRYFTQ
                     GW"
     gene            1644..1850
                     /locus_tag="ACZPAQ_07100"
     CDS             1644..1850
                     /locus_tag="ACZPAQ_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0903791.1"
                     /translation="MDEQLVLKNRLKVARAERGLSQGDLAKLVGVSRQTISSIETGQF
                     NPTAKLALVLCIALDKKFEELFYF"
     gene            complement(2044..>2324)
                     /locus_tag="ACZPAQ_07105"
     CDS             complement(2044..>2324)
                     /locus_tag="ACZPAQ_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903792.1"
                     /translation="CTGREIGPLIDANQELVRRRAGEIRDLITEPMDFSQICRAVCTR
                     FSLLTRRPRRALYYERNIRLFVEYLMDQGELGMETRQGTAFYLPSKKI"
BASE COUNT          416 a          724 c          674 g          510 t
ORIGIN      
        1 ggagaggggc cctgttcggc cggaacagcg actttctcac ggagctggaa gagcacaacg
       61 ccaactgcct ataccgtttt tccgacggcg gctattcttt tgtgggcaac accacctcct
      121 tcctccagat ggaggacggc gtcaatcagt tcggactggc ggcgggactc acctcggtgc
      181 cccccaaccc accccgcccc gggctgaatg ctggtctgac cctgcggctc ctgctggaat
      241 cctgccggga tgttccccag gccctggcgc tgctccggca gctccccgtc tcctcctgcc
      301 acacgctggt gctggccgac cgggctgggc gtatcgccct ggcggagtgt gccccggagc
      361 gcacccagat ccattgtccc ggggagacgg acgccttcgt ctgctccgtc aattccttcc
      421 acctctccgc catggccccc ttccgccggc agacggacga cgactggcgg gcggaagagc
      481 gctatctgac cctcacccgc tccctggccg cctggggccc ggagctcgac ctccaagctg
      541 gacaggacct gctctccggc aggatgggct tcctgtgcca gtatgaccgc agggagggcc
      601 gggacaccgt ctggtccgtg ctctatgaca cggcctccgg caccctcctg cgtgcggagg
      661 gcaaccccgg ccgcctcccc tttcagccag actccaggct ctcgtttcgt tcctgacctc
      721 atacggggag cggccctctg ccgttccccg tcatcttatt acgcttgtca tcttgcaaag
      781 tattattatt tttttgcaaa gtttacttga caaatttggg gtgacgtgat atgcttgtct
      841 caccaaacga aaggagcgct gctatgatgg acaaggaaga cattctgaag cggagccgcc
      901 gcgagaagga cgagggcctg acctatgccc aggaccgggg ccggcagtac ggcgtggtgg
      961 gatttctggt gctgtttctt gtcatcatga tctacaacat ggtccggggg ctggacaact
     1021 ccctgcccct gaccctgttt tgggcctatc tctcctgcga ggccgcaggc cgctactttg
     1081 cccggaagga aaagagcgtg ctggccaccg cagtgctggc cggactggcc tccctcttct
     1141 ccctggtctc ctacatcctg gagaccctgc cgtgagcccg gccagaccgc tggggccggc
     1201 caggctggta ctgacgtttg tgctgctgtc cgcccttttc ctgctggccc tgctggcggg
     1261 aatgctgcgg ctgctggtac tggcgctggt gcccgcccaa tgagaaaaga aaggagcatg
     1321 atccatgaac aaggaagaga tcctggcgaa aagccgcaaa gaaaacgtcc ttcaggacga
     1381 gatggagcgc actgtccgca tcgaggggga gtccttcagc ctgatcctgg tctttctggt
     1441 gggggtagtg atcgtgatct tcaaccggct ccacggcctg cctgccaaca gtgtgctggc
     1501 catgttctgg gcctcctgtg tgggcaaccg cctctaccgt ctcaccaagc ggaggaacac
     1561 ctccgacgtg gtgacgctgc tgatctctct tgcttttttg gcctggaatc tggtaagata
     1621 cttcacacag ggatggtgag gacatggatg aacagctggt cttgaaaaat cgcttaaagg
     1681 tggcccgggc ggagcgcggt ctgtcccagg gagacctggc caagctggtg ggcgtgtccc
     1741 gccagaccat cagctccatt gagaccggcc agttcaaccc caccgccaag ctggccctgg
     1801 tgctgtgcat cgcgctggac aaaaaatttg aggagctttt ctatttctga aataaatttt
     1861 tcgaattttt ggtttaattt ttctgtaatt tctcgatatt aaaccttgtt attatctacc
     1921 cgcaaaaatt atataatatt gaatatacgc cgaccagctg gcagcaatat gctcgttaac
     1981 ggtccgcaca gaatttaccg ggccccggag atcaaatatc tccggggccc ggttatactg
     2041 tcctcatatt ttctttgatg gcaggtagaa ggccgtcccc tggcgggtct ccatgcccag
     2101 ctccccctgg tccatcagat actccacgaa caggcggatg ttccgctcgt agtacagcgc
     2161 ccggcggggc cgccgggtga gcagggaaaa ccgggtgcat accgcccggc agatctggct
     2221 gaagtccatg ggctctgtga tcaggtcccg gatctctccg gcccggcggc gcaccagctc
     2281 ctggttggcg tcgatgaggg ggccgatctc tcttccggtg caca
//
LOCUS       DBMDSJ010000283         2309 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10815_length_2309_cov_1.544837, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000283 DBMDSJ010000000
VERSION     DBMDSJ010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10815_length_2309_cov_1.544837"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            248..1102
                     /locus_tag="ACZPAQ_07110"
     CDS             248..1102
                     /locus_tag="ACZPAQ_07110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903793.1"
                     /translation="MEDRAVRGIQTVIRYIESRLGGRLELETVAEAVHYSKYHLHRMF
                     TETVGMTLHDYVQRRRLTEAAKLLAFSRRPISQIALQCGYESQQSFSQAFKSMYKAPP
                     AAYRAAGNFYPLQLPFTLRMERGREAFRKEEIRLAEERDIPAWMELMRLVIDGYPAMD
                     EGAYLEQLRESIRAGHALVLGEGDTLAGALAFSTGPGRIEFLGVHPQYRRRGLQRLFL
                     DVLTETCLPDQEISTTTYRERDRADTGHRALLLELGFAQRELLTEFGYPTQRLVLLPR
                     GEEGRDDR"
     gene            1092..2306
                     /locus_tag="ACZPAQ_07115"
     CDS             1092..2306
                     /locus_tag="ACZPAQ_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0903794.1"
                     /translation="MTDNRQPSGWRKRALLFLTSQCITLFGSTLVQMALVWYAAMETA
                     SGGWVAAFSVCSYLPQFLVSFLGGVWADRYNRKALIIGADVLIACATLAMIPALPHIP
                     SGPALLGGLLVMSLLRSLGAGIQAPAVNAAIPQLVPEDQLMRYNGLNAAMQSLVNFAA
                     PAAAGAVFAVSDLRTTLMIDVVTAVVGTGLLSCLALPGQRGTRERSPLLADIRAGVSC
                     AFSHRLAGRLLVLYGLVTFFCVPGGYLAGLFVRRTFGETYWYLTAVELAGFAGMIAGG
                     VLMSTWGGFRRRGRTLTAGLLAFGVLAAGMGLSRHFVPYLGLMALYGTALTVVQTAIT
                     TMLQEHTEADMQGRVFGLLSTMYAGCLPLGMAIFGPLADVVSLRWIMAGAGGAILAAA
                     AAVGTDHRMRAV"
BASE COUNT          398 a          666 c          779 g          466 t
ORIGIN      
        1 tgttccagtc cataagtttc catggggcaa tcctcctatt ttcaacaaaa tttcttggaa
       61 aatttcgtga aaatattata ctccattttc cgaagaatgg gaagggcaag tttttcagga
      121 gctcatcagg gccaaaacaa gtcaggctgc acccttacat tttggaggaa aattttaaaa
      181 ccgcaagaat gaggaagcca ttttccatga aatctgttat cataccatcc agagaggagg
      241 ggcttcaatg gaagacagag cggtccgtgg gatacagacc gtcatcaggt atatcgagag
      301 ccgcctgggc ggcaggctgg aactggagac ggtggcggag gcggtccact actccaaata
      361 ccacctgcac cgcatgttca cagagacggt gggaatgacg cttcatgact atgtgcagcg
      421 ccgccggctc actgaggcgg ccaagctgct ggctttttcc cgacggccca tcagccagat
      481 cgctctgcag tgcggatacg agagccagca gtccttctcc caggcgttta agagcatgta
      541 caaggcgcct cctgcggctt accgggcggc tgggaacttc tatcccctcc agctgccctt
      601 taccctccgt atggagaggg gcagggaagc gttcagaaag gaggagatcc ggctggcaga
      661 agagagagat atccctgcct ggatggagct gatgcgtctg gtgatcgacg gctatcccgc
      721 catggacgag ggggcgtatc tggagcagct gcgggagagc attcgcgccg gacacgccct
      781 ggtcctgggg gagggtgaca ccttggcggg ggctctggcc ttctccacgg ggccgggacg
      841 catcgagttt ctgggggtgc acccccagta ccggaggcgg gggctccagc ggctgtttct
      901 ggacgtactg acggagacct gtctgcccga tcaggagatc agcaccacca cctaccggga
      961 acgggacagg gcggacacgg ggcaccgggc cctgctgctg gaactgggct ttgcccagcg
     1021 tgagctgttg acggagttcg gctaccccac ccagcgcctg gtgctccttc cccggggaga
     1081 ggaggggcgg gatgacagat aacagacagc cctccggatg gcgaaaaagg gctctgctct
     1141 ttctgaccag ccagtgcatc accctgttcg gctccaccct ggtgcagatg gccctggtct
     1201 ggtacgcggc tatggagacc gccagcggcg ggtgggtggc cgccttctct gtctgctcct
     1261 atctgccaca gtttctggtc tcctttttag gcggtgtttg ggcggaccgg tacaacagaa
     1321 aggcactcat catcggagcg gatgtcctga ttgcctgcgc caccttggct atgatccctg
     1381 cgcttcccca catcccatcg ggcccggccc tgctgggcgg cctgctggtc atgtcgctcc
     1441 tccgttccct gggggccggc atccaggccc cggcggtgaa cgcggccatt ccccagctgg
     1501 tcccggagga ccagctcatg cggtacaacg gcctcaatgc cgccatgcag tcgctggtga
     1561 atttcgccgc cccggcggcg gcaggcgcgg tattcgcggt gagtgatctg cgcaccaccc
     1621 tgatgatcga tgtggtgacg gcggtcgtgg ggacgggtct gctgtcctgc ctggcgctgc
     1681 ccggacagag agggactagg gagcggtctc ccctcctggc tgatatccgt gccggggtga
     1741 gctgtgcttt ctcccacagg ctggcgggga ggctgctggt actatacggg ctggtcacct
     1801 ttttctgcgt gccagggggg tatctggcgg gcctctttgt ccggcggacc tttggggaga
     1861 cctattggta tcttacagcg gtggagctgg ccggctttgc ggggatgata gcaggaggag
     1921 tgctgatgag cacctggggc ggcttccggc gcagggggag gaccctgacg gcgggcctgc
     1981 tggcttttgg cgtcctggcg gcagggatgg ggctgtcccg gcactttgtc ccctatctgg
     2041 ggctgatggc gctgtacggg acagcgctga cggtggtgca gacggcgatc accaccatgc
     2101 ttcaggagca cacggaggcg gacatgcagg gcagggtgtt cggactgctc agcaccatgt
     2161 acgccgggtg cctgcctttg ggcatggcca tctttggccc cctggccgat gtggtctctc
     2221 tgcggtggat catggcaggg gcggggggcg ccatcctcgc ggccgcggct gcggtgggca
     2281 ccgaccaccg gatgcgggcg gtgtgaacc
//
LOCUS       DBMDSJ010000284         2307 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10823_length_2307_cov_1.730734, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000284 DBMDSJ010000000
VERSION     DBMDSJ010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10823_length_2307_cov_1.730734"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="ACZPAQ_07120"
     CDS             <1..895
                     /locus_tag="ACZPAQ_07120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0903795.1"
                     /translation="PHPVYDLPPEEPRGVVQIVHGVGEHIGRYETAARFLTRQGFLVC
                     GEDHLGHGNTVQDGRYGAFAPQNGWKLVVADIARLRRIQGEKHPGVPYFLLGHSMGSF
                     LTRTYLIDYPGTVDGAILSGTGQEPAPLVAFGKLLAGLECRRLGYDGVSPLVDRLSLG
                     AYNRRFRPNRTSADWLSRDEEQVDAYLADPLCSHKSSVSMFRDMMGGLQYIARRENLA
                     RMDPDTPVYFFSGDQDPVGGMGKGVRKVYAMFQAAGCRDVTLKLYPGGRHEMFNELDR
                     QQVFDDLLAWLEAHMPREGRA"
     gene            complement(936..>2307)
                     /locus_tag="ACZPAQ_07125"
     CDS             complement(936..>2307)
                     /locus_tag="ACZPAQ_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903796.1"
                     /translation="LAALSCAFTLADTLLLAGSQERGGQLSYCPAALFGLFFLLHGTY
                     HKRCALRLSCRTAAAAAEPYLVTLDEGKWNGKDTYAKWSGTPAGFGSQIQMDDGAQRI
                     YRVFCPVLLLACIVFSLLASFGIGEGEHLMWCLSATFTAAAGFGGALAYGRSFHKVAR
                     RVSQSGGALAGWPGAAGSRRGNRVLITDLDLFPPGFVELNGIKVFGDFFVERVVGYTA
                     TLIRDSGCGLEKLFHDLLRTQGAIFRRADSLCCYEGGGLSANIRGDQVLVGSAAFMNL
                     MEVPLPQGLNVKNAVFCAIDGELAGIFALNYTLPDTVFPSLTSLLRERVGPVLATRDF
                     NLIPAMLQQRFKLAADRMDFPPVERRRELSDPEQDHTGVLTAVLCREGLLPFAESVVG
                     ARRLRRAVRASAVLTCAGSTLGVLLAYYLTSVDAYASLSPLNLLFYLLMWLLPVWFLS
                     GWVHRY"
BASE COUNT          422 a          743 c          829 g          313 t
ORIGIN      
        1 cccccacccc gtgtacgatc tgcccccgga ggagcctcgg ggcgtggtgc agatcgtaca
       61 cggggtgggg gagcacatcg gccgctatga gacagcggcc cgattcctga cccgacaggg
      121 ctttctggtg tgcggcgagg accacctggg acacgggaac acggtccagg acggccgcta
      181 cggcgccttc gccccccaga acgggtggaa gctggtagtg gccgacatcg cccgactgcg
      241 ccgcatccag ggggagaagc atccgggcgt cccctacttc ctcctgggcc actccatggg
      301 ctctttcctc acccggacct acctcatcga ctatccgggg acggtggacg gagccatcct
      361 gtcggggacc gggcaggagc ccgcccccct ggtggctttc ggaaagctgc tggccggact
      421 ggagtgccgc cggctggggt atgacggggt gagcccgctg gtggaccgcc tctccctggg
      481 ggcctataac cgcaggttcc ggcccaaccg gaccagcgcg gactggctct cccgggatga
      541 ggagcaggtg gacgcctatc tggccgaccc gctgtgcagc cacaagtcca gcgtgtccat
      601 gttccgggac atgatgggcg gtctccagta cattgcccgc cgggagaacc tggcgcggat
      661 ggacccggac acgccggtct atttcttctc tggggaccag gacccggtgg gcggcatggg
      721 aaaaggggtg cgcaaagtgt acgccatgtt ccaggcggcg ggctgccggg acgtgaccct
      781 gaagctgtat ccaggcggcc gacacgagat gtttaacgag ctggaccggc agcaggtctt
      841 tgacgacctg ctggcctggc tggaggccca catgccccgg gagggacggg cgtaaagaga
      901 ccgggcgcgc cgcgcagagc ggcgcgcccg gcttttcaat accggtggac ccagccgctg
      961 aggaaccaca caggcagcag ccacatcagc agataaaaca gcaggttcag gggggagagg
     1021 gaggcgtagg cgtccacaga agtcaggtag taggccagca gaacccccag ggtggagccg
     1081 gcgcaggtga gcaccgcgga ggcccggacg gcccggcgca ggcggcgggc ccccaccacc
     1141 gactcggcga aggggagcag cccctctcga cagagcacgg cggtgagcac gccggtgtgg
     1201 tcctgctccg ggtcggacag ctcccgccgg cgttccaccg gaggaaagtc catccggtct
     1261 gccgccagct tgaaccgctg ctggagcatg gccgggatga gattgaagtc ccgggtggcc
     1321 agcaccggcc caacccgctc ccgaagcagg gaggtgaggg agggaaacac ggtgtcgggc
     1381 agggtgtaat tcagggcgaa gatgccggcc agctccccgt cgatggcaca gaagaccgcg
     1441 tttttcacgt tcagcccctg gggcaggggc acctccatca gattcatgaa ggcggcggaa
     1501 cccaccagca cctggtcccc ccggatgttg gcggagaggc ctccgccctc atagcagcac
     1561 aggctgtccg ccctgcggaa gatggccccc tgggtgcgga gcaggtcgtg gaagagcttt
     1621 tccagcccac agcccgagtc ccggatcagg gtggcggtgt agcccaccac ccgttccacg
     1681 aagaagtccc caaagacctt gatgccgttg agctccacaa agccgggggg gaacaggtcc
     1741 agatcggtga tgaggacccg gttgccccgc cgggacccgg ccgcgccggg ccagccggcc
     1801 aaggcaccgc cgctctggga gacccggcgg gccaccttgt ggaaggagcg gccataggcc
     1861 agtgccccgc cgaagccggc ggcggcagtg aaggtggcgg agagacacca catcaggtgc
     1921 tccccctccc cgatgccgaa ggaggccagc agggaaaaga ctatgcaggc cagcagcagg
     1981 acgggacaga acacccggta gatgcgctgg gcgccgtcgt ccatctggat ctggctgcca
     2041 aagccggcgg gggtgccgga ccacttggcg taggtgtcct tcccgttcca cttgccctcg
     2101 tccagagtga ccagataggg ctccgcggcg gccgctgcgg tgcggcagga caggcgaagg
     2161 gcgcaccgct tgtgataggt gccgtggagc aggaagaaca gaccgaacag ggcggcggga
     2221 cagtaggaga gctgcccgcc ccgttcttgg ctgccggcca gcagcaaggt atccgccagg
     2281 gtgaaggcgc aggagagggc ggccagg
//
LOCUS       DBMDSJ010000285         2294 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10915_length_2294_cov_2.606019, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000285 DBMDSJ010000000
VERSION     DBMDSJ010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10915_length_2294_cov_2.606019"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="ACZPAQ_07130"
     CDS             complement(<1..548)
                     /locus_tag="ACZPAQ_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR0903797.1"
                     /translation="MELWYALVDLLPFDWAQPGSMYFMKNALLAVLVISPLFGVLSTM
                     VVHSRMSFFSDALGHSAFTGMAIGAICGFNEPTWAAVLFAVVFALLFNLVRRRSALAS
                     DTVIGVFSSTAVALGIFLSTLGGRSFTKFNSLLIGDILSVEPEKIALLAGILLVVLVL
                     WGLSFNQLMLSAVHPALADSRGV"
     gene            complement(548..1351)
                     /locus_tag="ACZPAQ_07135"
     CDS             complement(548..1351)
                     /locus_tag="ACZPAQ_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR0903798.1"
                     /translation="MRRLKHGKMSGGCGDSCCLKLEGLNVRIQGEEILEDVSFHLHCG
                     EITALIGPNGAGKSTLFRSILGQLPYSGSITFAPAGGPAIRLADGTVMGGTRPLIGYV
                     PQSPSFDRGDPVSVLDFFTAATARWPVCLPIPGKYRERCRRSLERVHGGDLLDKPMGG
                     LSGGQLQRVLLALALEPVPHILVLDEPLSGVDIEGEHQLLEMLDELRTEYDLSILLST
                     HDFATLGQFADKVILLNKRVLRMGPPEEVTSSPEFYETFHLRMGKGGEG"
     gene            complement(1348..>2294)
                     /locus_tag="ACZPAQ_07140"
     CDS             complement(1348..>2294)
                     /locus_tag="ACZPAQ_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR0903799.1"
                     /translation="LVTLLSLLLPGCAPAGTGGTDGEEARLTVLASTYPVYLAARSVA
                     EGVEGVSVLRLETGAVSCLHDYTLTVGDMKKLEGADVVALNGAGMEEFMEDALASSSA
                     PVIDCSEGVDLLEGEGHVHQDGEEDDDHGHFDPHYWMDPENMEQMVYNIRDGLSQADP
                     EHAGEYEENAAEQAGLLHTWDDALQDMIQEAEENAGVEISGLITFHDGFRYFAEAFDL
                     PLLASIEEEEGSEASAKEINEIAGLVKEYSLPVIFTEVNGSDATAQAIARETGCAVAQ
                     LSMCMDGPEEGTLSDYYTVLADNVTAVVNGFAGEEVIR"
BASE COUNT          415 a          799 c          729 g          351 t
ORIGIN      
        1 accccccggc tgtccgccag ggcggggtgg acggcagaga gcatcagctg gttgaaggac
       61 agcccccaca ggaccagcac caccagcagg atccccgcca gcagggcaat cttctccggc
      121 tccaccgaga ggatgtcgcc gataagcagg ctgttgaact tggtaaagga gcgccccccc
      181 agggtggaga ggaagatgcc cagggccacc gcggtggagg agaacacccc gatcaccgtg
      241 tcgctggcca gggcggagcg gcggcgcacc agattgaaca gcagggcaaa caccaccgcg
      301 aagagtaccg ccgcccaggt gggctcgttg aagccgcaga tggccccgat ggccatgccg
      361 gtaaaggcgg agtgccccaa ggcatcggag aaaaaggaca tccggctgtg gaccaccatg
      421 gtggacaaca caccaaacag gggcgagatg accagcaccg ccagcagggc gtttttcata
      481 aagtacatgg atccgggctg ggcccagtca aagggcagca gatccaccag cgcgtaccag
      541 agctccatca gccctcgccc cccttcccca tccgcagatg gaaggtctca taaaactccg
      601 gagaggaggt gacctcctcc ggcggcccca tgcggagcac ccgcttgttc agcaggatca
      661 ccttgtcggc gaactgcccc agggtggcaa agtcgtgggt ggacagcagg atggacaggt
      721 cgtactccgt ccgcagctcg tccagcatct ccagcagctg gtgctccccc tcgatgtcca
      781 cgccggacag gggctcgtcc aggaccagga tgtggggcac cggctccagg gccagggcca
      841 gcagcacccg ctggagctgc cccccggaca gcccccccat gggcttgtcc agcaggtcgc
      901 cgccgtggac ccgctccagg ctgcggcggc agcgctcccg gtacttcccg gggatgggca
      961 gacataccgg ccaccgggcg gtggcggcgg tgaaaaagtc cagcaccgac accggatccc
     1021 cacggtcgaa gctgggggac tggggcacat agccgatgag gggccgggtc ccccccatga
     1081 ccgtcccgtc ggccagacgg atggcgggcc cccccgccgg ggcaaaggtg atgctcccgg
     1141 agtagggcag ctggcccagg atgctgcgga acagggtgga cttgcccgcc ccgttggggc
     1201 cgatgagggc ggtgatctcc ccgcagtgga ggtggaagga cacgtcctcc aggatctcct
     1261 ccccctggat gcggacgttc agcccctcca gcttcagaca gcaggagtcc ccgcacccgc
     1321 cggacatctt tccgtgcttg agtcttctca tcgaatcact tcttccccgg caaacccgtt
     1381 taccacggcg gtcacattgt ccgccagcac ggtgtagtag tcggacagcg tcccctcctc
     1441 cgggccgtcc atgcacatgg acagctgggc cacggcgcac cccgtctccc gggcgatggc
     1501 ctgggcggtg gcgtcggagc cgttgacctc ggtgaagatc accggcaggc tgtattcctt
     1561 tacaagccct gcgatctcat tgatctcctt ggcggaggcc tcactcccct cctcctcctc
     1621 aatggaggcc agcaggggca ggtcaaaggc ctcagcaaaa tagcggaatc cgtcgtggaa
     1681 ggtgatcagg ccgctgatct ccaccccggc gttctcctcc gcctcctgaa tcatatcctg
     1741 gagagcgtcg tcccaggtgt ggagcagacc ggcctgctcc gcggcgttct cctcatactc
     1801 ccccgcgtgc tccgggtccg cctgggagag gccgtctctg atgttgtaca ccatctgctc
     1861 catattctcc gggtccatcc agtagtgggg gtcgaagtgg ccgtggtcat catcctcctc
     1921 cccgtcctgg tggacatggc cctcgccctc caacagatcg actccctcgg agcagtcgat
     1981 gaccggggcg ctggaggagg ccagggcgtc ctccataaat tcctccatgc cggccccgtt
     2041 cagggccacc acgtcggccc cctcaagctt cttcatgtcc cccacggtga gggtgtagtc
     2101 gtggaggcag gacaccgccc ccgtctccag ccggaggacg gagacgccct ccaccccctc
     2161 cgccaccgac cgggcggcca ggtagaccgg ataggtggag gccagcaccg tcaggcgggc
     2221 ctcctccccg tccgttccgc cggtcccggc gggggcgcag cccggcagca gcagggacag
     2281 gagggtcaca agag
//
LOCUS       DBMDSJ010000286         2292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_10925_length_2292_cov_2.189411, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000286 DBMDSJ010000000
VERSION     DBMDSJ010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10925_length_2292_cov_2.189411"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="ACZPAQ_07145"
     CDS             complement(<1..924)
                     /locus_tag="ACZPAQ_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR0903800.1"
                     /translation="MFGDFLYLLRRSGLKISLTEWMALMEALDKGLEGASLTGFYYLC
                     RALLVKTEADFDRFDRAFLEYFKDVPFEQEVSQELLDWLDRPDALMGRYDEQQAELNE
                     LLSNTEIEEMFRERMNEQREQHNCGNYWVGTHGMSVFGNSGMSPNGIRVGGEGQGKRA
                     FRVAGERRFRDFRGDNTLDTRQFQVALRHLRQFSGLVDLPATEFDVDNTIKDTANNAG
                     SLKVRYKKPRQNTVKVLLLMDSGGSMEYYSRLCSALFQAVSKSGHFKDLKVYYFHNCV
                     YSRLYTEPTLRPSNTVPTDWVLSNLSSEYKVI"
     gene            complement(929..1741)
                     /locus_tag="ACZPAQ_07150"
     CDS             complement(929..1741)
                     /locus_tag="ACZPAQ_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0903801.1"
                     /translation="MSETFQGSQDYVASEELMRAVNIAMVLQKPLLIKGEPGTGKTVL
                     AEAIAKSLGKELIIWNIKSTTKAQDGLYVYDVVQRLYDSQFGGEGVDDIEKYVKLGKL
                     GEAFTADDQVILLIDEIDKADLEFPNDLLWELDRMEFHIPETGRTVTAKHRPIVIITS
                     NAEKELPDAFLRRCVFHYIEFPDRELMAEIVNVHFQDLDQHLLDQVLEAFYRIRSLPQ
                     IKKKPSTSEIIDWIQALIHGGYDPDRVVAEVPYLGVLLKKNEDIDALRKSWR"
     gene            2024..>2292
                     /locus_tag="ACZPAQ_07155"
     CDS             2024..>2292
                     /locus_tag="ACZPAQ_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyltransferase"
                     /protein_id="HIR0903802.1"
                     /translation="MLHTQLHGTHWQIGFQLGAGLARDGHFILDAVPFPITPARLEFA
                     RTCLPLCRRWFPAALEELRGLAQGQGCAPDELAGVLFSMYAMPPGP"
BASE COUNT          413 a          716 c          671 g          492 t
ORIGIN      
        1 gatgaccttg tactcgctgg acaggttgct cagcacccag tcggtgggca ccgtgttgct
       61 gggacggagg gtgggctcgg tgtacaggcg ggagtagacg cagttgtgga aatagtacac
      121 cttcagatcc ttgaaatggc ccgacttgct caccgcctgg aacagagcgg agcacaggcg
      181 gctgtaatac tccatggacc cgccggagtc catgagcagc agcaccttca ctgtgttctg
      241 ccggggcttt ttatagcgca ccttcaggga gccggcgttg ttggcggtgt ccttgatggt
      301 gttgtccaca tcgaactcag tggcaggcag gtccaccagg ccggagaact gccgcagatg
      361 gcgcagggcc acctggaact ggcgggtgtc cagggtgttg tccccccgga aatcccggaa
      421 gcggcgctcg cccgccaccc ggaaggcccg cttgccctgg ccctcgcccc ccacccggat
      481 gccgttgggg ctcatgcccg agttgccgaa gacggacatg ccgtgggtgc ctacccagta
      541 gttgccgcag ttgtgctgct cccgctgctc gttcatccgc tctcggaaca tctcttcgat
      601 ctctgtgttg gacagcagct cattgagctc ggcctgctgc tcgtcatacc ggcccatcag
      661 agcatcgggg cggtccagcc agtccagcag ctcctgggac acctcctgct cgaagggcac
      721 gtccttgaag tactccagga aagcccggtc aaagcggtcg aagtcggcct ccgtcttcac
      781 cagcagtgcc cggcacaggt agtaaaagcc agtgaggctg gccccctcca gccccttgtc
      841 cagggcttcc atcagagcca tccactcggt gagggagatc ttcaggccgc tgcggcgcag
      901 cagatagaga aaatcaccga acataccatc accgccagct cttgcgcagg gcgtcgatgt
      961 cctcgttctt cttcagcagc acgcccagat agggcacctc ggccacgacg cggtcggggt
     1021 catagccccc gtggatcagg gcctgtatcc agtcgatgat ctcgctggtg gagggctttt
     1081 tcttgatctg gggcaggctg cggatgcggt agaaggcctc cagcacctgg tccagcagat
     1141 gctggtccag atcttggaag tgcacgttga cgatctctgc catcagctcc cggtctggga
     1201 actcgatgta gtggaatacg cagcggcgca ggaaggcgtc gggcagctcc ttctccgcat
     1261 tggaggtgat aatgacgatg gggcggtgct tggcggtgac ggtgcgtccc gtctccggga
     1321 tgtggaactc catccggtcc agctcccaca gcaggtcgtt ggggaactcc agatcggcct
     1381 tgtcgatctc gtcgatgagc aggatcacct ggtcgtcggc ggtgaatgcc tcccccagct
     1441 tgcccagctt gacatacttc tcgatgtcgt ccacgccctc gccgccgaat tggctgtcat
     1501 acagccgctg gaccacgtcg tagacataca gaccgtcctg ggccttggtg gtggacttga
     1561 tattccagat gatcagctct ttgcccaggg atttggcgat ggcctcagcc agcaccgtct
     1621 ttcccgtgcc cggctcccct ttgatcagca ggggcttctg gagcaccata gcgatattca
     1681 ccgcccgcat cagttcctcg gaagcgacat agtcctggct tccctgaaat gtctcactca
     1741 tagcagtatc ctttcccggc ccgggggcca tttcatcatt tgacatagat atttggaatt
     1801 ttatcgtttt ttccccgcaa atgcaagccc ttcccgtcca aaatccaaaa tttcaaagtc
     1861 ttttgcagga gcggggccgg gccagccctc caaaaacggc ctggaataaa aaaatttaaa
     1921 aaataaatcg gccttgctcc aatattttaa ttgtgctacc gttcttttga gaggcggcgc
     1981 cgcgctcttt actctgagat ctgaacagaa caggagcgtt tttatgcttc acacacaact
     2041 gcacggcaca cactggcaga tcggcttcca gctgggagcc ggtctggccc gggacggcca
     2101 ttttatcctg gacgctgttc cctttcccat caccccggcg cggctggagt ttgcccggac
     2161 ctgcctgccc ctctgccgcc ggtggttccc cgccgccctg gaggagctgc gggggctggc
     2221 ccaggggcag gggtgtgccc cggacgaact ggccggggtc ctcttctcca tgtacgccat
     2281 gccgcccggc cc
//
LOCUS       DBMDSJ010000287         2274 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11024_length_2274_cov_1.803497, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000287 DBMDSJ010000000
VERSION     DBMDSJ010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11024_length_2274_cov_1.803497"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="ACZPAQ_07160"
     CDS             <1..184
                     /locus_tag="ACZPAQ_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR0903803.1"
                     /translation="YVHGGCSPIGMKKQFPTVFHETAEILDTIMVSAGKIGFQVELSP
                     ADLIELVGGTTADVTV"
     gene            256..729
                     /locus_tag="ACZPAQ_07165"
     CDS             256..729
                     /locus_tag="ACZPAQ_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025569.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HIR0903804.1"
                     /translation="MEQQEKPPKFLYLPEVKNDHFIIGGADDRPDASVPGSEPGPGPE
                     EPTGIYPCPCCGYLTFPAPKEEAIACICPVCFWENDVFDPGEDDPSDENRGLTLRQGR
                     ENYRRWGAVRPDLVVHARPPRPEERPKEMPASRLTAPPGTGTAPGRGKAPGPGPA"
     gene            complement(803..934)
                     /locus_tag="ACZPAQ_07170"
     CDS             complement(803..934)
                     /locus_tag="ACZPAQ_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903805.1"
                     /translation="MYDTLSLLYELVIQKFPYEPRSPTEDGLLERLEQAQEKVRSCS"
     gene            1018..1245
                     /locus_tag="ACZPAQ_07175"
     CDS             1018..1245
                     /locus_tag="ACZPAQ_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903806.1"
                     /translation="MEKIMLPLGRRLKELRKERKLKQKEMARFLECTDRNYQKMEYGE
                     INVPGLTLIKLADFFQVSLDYLVGRSDDREQ"
     gene            1423..>2274
                     /locus_tag="ACZPAQ_07180"
     CDS             1423..>2274
                     /locus_tag="ACZPAQ_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR0903807.1"
                     /translation="MHSHIFVKGARVNNLKNIDVDIPRDKLVVLTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARMFLGQMEKPDVDYIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLWARVGTPHCPICGKEIKQQTIDQIIDQVMALPEATRIQIMAPVIRGKK
                     GEHAKIFEDARKSGYVRCRVDGIAYDLSEEIKLEKNKKHDIEIVVDRLVIREDIARRL
                     TDSVETAASLAGGLVIVNVVGEDRDILFSQNYACEDCGVSIEELTPLMFSFNNPFGAC
                     PTCTGLGS"
BASE COUNT          512 a          666 c          702 g          394 t
ORIGIN      
        1 ctatgtccac ggggggtgct cccccatcgg catgaagaag cagttcccca ccgtcttcca
       61 tgagacggcg gagatcctgg acaccatcat ggtctccgcc ggaaagatcg gcttccaggt
      121 ggagctgtcc ccagcggacc tgatcgagct ggtgggcggg accacggcgg acgtgacagt
      181 gtgaggaaaa gaaactgata tacccagatg ttctgcaatc ggccggctgt gaccgctttg
      241 tgaggagggg ataagatgga gcagcaggaa aagccgccca agtttctcta tctcccagag
      301 gtcaagaatg accatttcat catcggcggg gcggatgacc gccccgatgc gtccgtaccg
      361 ggatcagagc cggggccggg acctgaggag cccacgggta tctacccctg cccgtgctgc
      421 gggtatctga cctttcccgc ccccaaggag gaggccatag cctgcatctg tccggtgtgc
      481 ttctgggaaa acgacgtgtt cgacccgggg gaggacgacc caagcgatga gaaccgcggc
      541 ctgaccctcc gccagggccg ggaaaactac cggcgctggg gcgcggtccg ccccgacctg
      601 gttgtccacg cccgaccgcc caggccggag gagcggccga aagaaatgcc ggcctcccgg
      661 cttacggctc ctcctggaac tgggacagct cccgggagag ggaaagcccc aggtccaggc
      721 ccagcgtgaa ggcgtggcag gcctggaagg aggctaagat ctccatgctg tcagtgagat
      781 ggaagagcag atcctggtcg gcctaggaac aggagcggac cttctcctgg gcctgctcca
      841 gccgctccag aagcccgtcc tctgtggggg agcggggctc ataggggaat ttttggatga
      901 ccagctcata gagcagggaa agtgtgtcat acatagagta ccacccgaaa gtaataattt
      961 aattatgact aaattatagg cgaatttaga attggtgtca agagggagat tggcaggatg
     1021 gaaaagatca tgctgcccct ggggcggcgg ctgaaagaac tgcggaaaga aagaaaactc
     1081 aaacaaaagg aaatggcccg gtttttggaa tgtacagacc gaaattatca gaaaatggag
     1141 tacggagaga ttaacgttcc cggactgaca ctgatcaaac tggcggactt ctttcaggtc
     1201 agcctggact acctggtggg ccgcagcgac gaccgggagc agtaaaaacg atccccgccc
     1261 cccccccgcc gggcgggaag agcagggaaa agccgggggg ctgatcttgg cgggcgtgtg
     1321 cctgatcctc tgtagaatga acattcaagc catttcccag tcaaacctcg accccagtgt
     1381 catctgcctg cgcatacagg cgttaccaaa ggagaaacca aaatgcacag tcatattttt
     1441 gtcaagggcg cccgggtgaa caacctgaaa aacattgatg tggacatccc gcgggacaag
     1501 ctggtggtcc tcaccgggct gtccggttcg ggaaaatcct ccctggcctt cgacaccatc
     1561 tatgccgagg gacagcggcg gtatgtggag tccctcagct cctacgcccg catgttcctg
     1621 ggccagatgg agaagcccga cgtggactat atcgacgggc tctcacccgc catctctatc
     1681 gaccagaaga ccaccagcaa gaacccccgc tccactgtgg gcaccgtgac ggagatctat
     1741 gactacctcc gtctgctgtg ggcccgggtg ggcacccccc actgccccat ctgcggcaag
     1801 gagatcaaac agcagaccat cgaccagatc atcgatcagg tgatggcgct gcccgaggcc
     1861 acccgcatcc agatcatggc cccggtgatc cggggcaaga agggggagca cgccaagatc
     1921 ttcgaggacg cccgcaagtc gggctacgtc cgctgccggg tggatggcat cgcctatgat
     1981 ctcagcgagg agattaagct ggaaaagaac aagaagcacg acattgagat cgtggtggac
     2041 cgtctggtga tccgggagga catcgcccgc cgtctcacag attcggtgga gaccgccgcc
     2101 tccctggcgg gggggctggt gatcgtcaac gtggtggggg aggaccggga tatcctgttt
     2161 tcccagaact acgcctgcga ggactgcggc gtctccatcg aggagctcac ccccctcatg
     2221 ttctccttca acaacccctt cggcgcctgc cccacctgca ccggcctggg cagc
//
LOCUS       DBMDSJ010000288         2270 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11044_length_2270_cov_1.556017, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000288 DBMDSJ010000000
VERSION     DBMDSJ010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11044_length_2270_cov_1.556017"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="ACZPAQ_07185"
     CDS             <1..978
                     /locus_tag="ACZPAQ_07185"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR0903808.1"
                     /translation="LSRVERPNGLVIEKTAVPMGVIAIIYESRPNVTSDAAALALKAG
                     SACVLRCGKEAHRSAAAIVSALCRGLSRAGLPQEALCLVEDTTRASAHELMTASGYVD
                     LLIPRGGAGLIRACVDNATVPVIETGTGICHVYVDRAADLDMALDILENAKCSRPSVC
                     NAAEVCLVHREAAPVFLPRMRERLTDARAARGERPVELRLDGTAAGIIPGTPAGPEDF
                     DTEFLDYILAVRVVDGTEEAIRHIAAHSTGHSEAIVTEDGEAARRFLAAVDSAAVYWN
                     ASTRFTDGGEFGLGCEMGISTQKLHARGPLGLAELCSFKFVIRGGGQIR"
     gene            1174..1683
                     /locus_tag="ACZPAQ_07190"
     CDS             1174..1683
                     /locus_tag="ACZPAQ_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0903809.1"
                     /translation="MHKHLLEFCRLTGELLESEPVRMMGRWQHHGRITTLDHSLFVAY
                     LSFRAARWLRLDERAAARGGLLHDLYLYDPRDKSAHPGSQCFDHPRAAARNAEQVTKL
                     SDKERNIILSHMWPLGGALPRSLEALLVDLVDTFCAGLELSHIYHPGRLREKLGVVPL
                     AGSPQADAA"
     gene            2014..>2270
                     /locus_tag="ACZPAQ_07195"
     CDS             2014..>2270
                     /locus_tag="ACZPAQ_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HIR0903810.1"
                     /translation="MIAHGKDIKVFTGNSNPKLAQNICQELGIPLGDSEVGAFSDGEN
                     FASIYETVRGSDVFVVQSTCSFRKDDKSGSVNDALMELLIM"
BASE COUNT          403 a          738 c          737 g          392 t
ORIGIN      
        1 ctcagccggg tggagcggcc caacgggctg gtgatcgaga agaccgccgt cccaatgggg
       61 gtcatcgcca tcatctatga gagccggccc aatgtcacca gcgacgccgc agccttggcc
      121 ctgaaggcgg gctccgcctg tgtgctccgc tgcgggaagg aggcccaccg ctccgccgcg
      181 gccatcgtct ccgccctgtg ccggggcctc tcccgggccg gactgcccca ggaggccctg
      241 tgtctggtgg aggacaccac ccgggcctcc gcacacgagc tgatgaccgc cagcggatac
      301 gtggatctgc tcatcccccg ggggggcgcg gggctgatcc gggcctgtgt ggacaatgcc
      361 accgtccccg tcatcgagac gggcaccggc atctgccacg tctatgtgga ccgggcggcg
      421 gacctggaca tggcgctgga catcctggag aacgccaagt gcagccggcc cagcgtgtgc
      481 aacgccgccg aggtgtgcct agtccaccgg gaggcggccc cggtctttct gccccggatg
      541 cgggagcggc tgacggatgc cagggcagcc cggggggagc ggccggtgga gctgcgcctg
      601 gacgggacgg cggccgggat catccccggc acgcccgcgg gtccggagga cttcgacact
      661 gagtttttgg actatatcct ggctgtccgg gtggtggacg ggacggagga ggccatccgc
      721 cacatcgcag cccactccac cggccactcg gaggccatcg tgacagagga cggggaggcg
      781 gcccggcgct tcctggccgc ggtggactca gcggcggtgt actggaacgc ctccacccgc
      841 ttcaccgacg ggggggagtt tggcctgggc tgcgagatgg gcatctccac ccagaagctc
      901 cacgcccggg gccccctggg gctggccgag ctgtgcagct tcaagttcgt gatccggggc
      961 gggggacaga tccgctgagg cgccccctct cttttccagc tgacaaatgc gcggtctccg
     1021 ggtatgatgg gagcagagcg acactctcat cagagccgtg agccagatat cgcggggacg
     1081 gcccagaacc gcccgccgcg gcatcccatt tccgcccccc ttttcccaac tgggtgaaag
     1141 ggaggaaaat acccatgaaa ggactgatat ccgatgcaca agcatttgct ggagttctgc
     1201 cgcctgacgg gagagctcct ggaaagcgag ccggtgcgga tgatgggccg ctggcagcac
     1261 cacggccgga tcaccaccct ggaccactcc ctgttcgtgg cctacctgtc cttccgggca
     1321 gcccggtggc tgcgtctgga tgaacgggcc gccgcccgcg ggggcctgct ccacgacctg
     1381 tacctctatg acccccggga caagtccgcc caccccggca gccagtgctt cgaccacccc
     1441 agggccgcag cccggaatgc cgagcaggtg accaagctct ccgataagga gcgcaacatt
     1501 atcctgtccc acatgtggcc cctgggcggg gcactgcccc ggtccctgga ggccctgctg
     1561 gtggacctgg tggatacctt ctgcgcgggg ctggagctct cccacatcta tcacccaggc
     1621 cgcctgcggg agaagctggg cgtggtcccc ctggcgggtt ccccccaggc cgacgcggcc
     1681 tgatctgctc cgcacctcct catgccccgg cagcgtctgc tgccggggca tttttagaca
     1741 tttttaggaa aagagaggca ggaataaaaa ttcccggaaa attttcgaaa atttctcgaa
     1801 aaattctctt gaacccagga aactttcttg ctattatgct ataatggttg ggggtatccg
     1861 cataacgtgc cggatctgtg cagtttgatg gagaagcccc acgcatatcc gtgctgatcg
     1921 gcactgccgt cacaatagag gaacagagag caagcagatt tggtgaccga cccggccagg
     1981 taccggcggt ccgcgtaagg aaggaagttg tttatgatcg cacacgggaa ggatatcaag
     2041 gtattcaccg gaaactccaa ccccaagctg gcccagaaca tctgtcagga gctgggcatc
     2101 cctctgggcg actccgaggt gggagccttc tccgacgggg agaatttcgc ctctatctat
     2161 gagaccgtcc gcggctccga cgtctttgtg gtgcagtcca cctgctcctt ccgcaaggac
     2221 gacaagagcg gttccgtcaa cgatgcgctg atggagctgc tcattatgat
//
LOCUS       DBMDSJ010000289         2262 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11098_length_2262_cov_2.027765, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000289 DBMDSJ010000000
VERSION     DBMDSJ010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11098_length_2262_cov_2.027765"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(124..486)
                     /locus_tag="ACZPAQ_07200"
     CDS             complement(124..486)
                     /locus_tag="ACZPAQ_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903811.1"
                     /translation="MNKVSFEEIGAVAATFFAQKEVKAGQVVKMTGNGQVGPCSAGDK
                     FCGVALSNENGCAGVQVKGFCPVAVTGSIDLGWNVLAADGAGGVQTATAGAEESPSGV
                     ECLVVSTDTAGKRAVICL"
     gene            complement(529..1455)
                     /locus_tag="ACZPAQ_07205"
     CDS             complement(529..1455)
                     /locus_tag="ACZPAQ_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903812.1"
                     /translation="MKVEKEALGGQRVEPTEEELALVNRQSRKELTAAEVYLFAVRLC
                     DNEVDRDGEQFPQATLEQLAELFVGKSGIFDHRWSAGGQTARIYRTEVVREEGIRTAL
                     GEPACYLKGYAYMVRTQGNQDLIAEIEGGIKREVSVGCAVKRAECSICGSDMGRCAHE
                     KGKRYDGRLCCARLLEATDAFEFSFVAVPAQPRAGVMKRAGGSPSLKQLALGEPAAAA
                     ELEDLERAAELGRRYLTGLRNEVVRLGLLAERQVGAETLRHIADRLEEKELLELREDY
                     QRRAAERYPIAPQLSYGNGDGMPERPDGAFLI"
     gene            complement(1452..>2262)
                     /locus_tag="ACZPAQ_07210"
     CDS             complement(1452..>2262)
                     /locus_tag="ACZPAQ_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HIR0903813.1"
                     /translation="GVGELVLDCQGREIAALLCAGPEQVEIREGDSPLDFQLCARGPG
                     GTCEPLPWQELLLFTPFQPETEHPYGVSLLRSMPFLTDILLKIYQATGMNWERMGNVR
                     FAVICRPGEGEGAYAQERCRQIAGEWSAAMQAGKQGAVRDFVAVGDLDIKVIGADNQV
                     LDSQVPVRQILEQLVARTGIPPFLLGLSWSSTERMSAQQADLMTSEITALRRTLEPAV
                     ERICEMWLRLHGWGGDVTVDWADINLQDFVEEARAKLYLAQAEAIREEERT"
BASE COUNT          349 a          898 c          609 g          406 t
ORIGIN      
        1 ctgctccgag gggtcccggc tctccagcac ctgagtgaag gaccgccccg cctcatgata
       61 catccccttc tccagcttca gattgtcgta gcaataggcc atctcagctc tctcctctct
      121 gtctcacagg cagatcacgg cgcgcttgcc cgccgtgtcc gtactgacca ccagacactc
      181 caccccagat ggggactctt cggccccggc ggtcgccgtc tggacaccgc cggccccatc
      241 cgccgccagc acgttccacc ccaaatcgat ggagcccgtc accgccaccg ggcagaatcc
      301 cttcacctgt acgccggcgc acccgttctc attggacagg gccacgccgc agaacttgtc
      361 cccggccgag cagggcccca cctggccgtt gcccgtcatc ttcaccacct gtcctgcctt
      421 gacctccttc tgggcgaaaa aggtggccgc cactgcgccg atctcctcaa aggaaacctt
      481 gttcattccc gatccctccc gatttatttt gtaaagacgg ccccgccgtc agatcaaaaa
      541 agccccgtcc ggccgctccg gcatcccgtc cccgttaccg taggacagct gcggggcgat
      601 ggggtagcgc tccgccgccc gccgctgata atcctcccgc aactccagca gctccttttc
      661 ctccagccgg tccgcgatgt gccggagggt ctccgcccct acctggcgct ccgccagcag
      721 gcccagccgg accacctcgt tccgcagtcc cgtcagatag cggcgcccca gctcggccgc
      781 ccgctccaga tcttccagct cggctgcggc ggcgggctct cccagcgcca gctgcttcag
      841 cgaagggctc cctccggccc gcttcatcac tccagccctg ggctgggcgg gcactgcgac
      901 aaaggagaac tcgaaagcgt ctgtggcctc cagcaaacgc gcgcagcaca gcctgccgtc
      961 gtaccgcttt cccttctcgt gggcgcacct gcccatgtcc gacccgcaga tggagcactc
     1021 tgcccgcttt accgcgcagc ccacgctgac ctcccgcttg ataccccctt cgatctccgc
     1081 gatcaggtcc tggtttccct gggtccgcac catgtaggcg taccccttca gatagcaggc
     1141 gggctctccc agcgccgtgc gtatcccctc ctcccggacc acttccgtcc ggtagatgcg
     1201 ggccgtctga ccgccggcgc tccacctgtg gtcaaagatc ccgctcttgc ccacaaacag
     1261 ctccgccagc tgctccaggg tggcctgtgg aaactgctcc ccgtcccggt ccacctcgtt
     1321 gtcgcacagc cgcaccgcga acagatagac ctccgccgct gtcagctcct tccggctctg
     1381 ccggttgacg agggccagct cctcctctgt gggctccacc ctctggcccc caagggcctc
     1441 cttttctacc ttcatgtccg ttcctcctct ctgatggctt ccgcctgggc cagatacagc
     1501 ttcgctctgg cctcctccac gaagtcctgc aagttgatgt ctgcccagtc caccgtcaca
     1561 tcgccgcccc agccgtggag ccgcagccac atctcacaga tgcgctccac cgccggctcc
     1621 agggtccggc gcagggcggt gatctcgctg gtcatcagat cggcctgctg ggcgctcatc
     1681 cgctccgtgc tggaccagga cagccccagc aggaagggag ggataccggt gcgggcaacg
     1741 agctgttcca ggatctgacg caccggcacc tggctgtcca gcacctgatt gtccgccccg
     1801 atgaccttga tgtccagatc ccccaccgcc acaaagtccc gcacggcccc ctgcttgccc
     1861 gcctgcatgg cggcggacca ctccccggcg atttgccggc accgctcctg ggcataggct
     1921 ccctctccct ctccggggcg gcagatgacc gcgaagcgca cgttgcccat tcgctcccag
     1981 ttcatccccg tggcctggta gatcttcagc aggatgtcgg tgaggaaggg catggaccgc
     2041 agcagagaca ccccgtaggg gtgctctgtc tccggctgaa aaggggtgaa cagcagcagc
     2101 tcctgccagg gcaggggctc acaggttccg cccgggcccc gggcacacag ctgaaagtcc
     2161 aggggcgagt ctccctcccg gatctccacc tgctccggtc cggcgcacag cagagcggcg
     2221 atctcccggc cctggcagtc cagcaccagc tcgcccaccc cc
//
LOCUS       DBMDSJ010000290         2247 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11188_length_2247_cov_1.595993, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000290 DBMDSJ010000000
VERSION     DBMDSJ010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11188_length_2247_cov_1.595993"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            74..520
                     /gene="dtd"
                     /locus_tag="ACZPAQ_07215"
     CDS             74..520
                     /gene="dtd"
                     /locus_tag="ACZPAQ_07215"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR0903814.1"
                     /translation="MTRVSSASVSIGGTVEGAIEQGFLVLLGVGPGDTEAVCDKLAEK
                     ICNLRVFEDENGRMNLSLEQVGGALLVVSQFTLYADTSSRRPGFTGAARPDLAVPLYE
                     RFMEQCRRRGFQVEHGTFGADMQVASVNDGPVTILFDTDKNGAGRA"
     gene            586..1200
                     /locus_tag="ACZPAQ_07220"
     CDS             586..1200
                     /locus_tag="ACZPAQ_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0903815.1"
                     /translation="MKVKLLRVGPIGTNCYILEDDQTNLAAVIDPGDEPELIQEALEK
                     EGVEVRYLLLTHGHYDHTTAVPALHRVYPQADIYIHQADANGAGSTLFPLAGEVDDLK
                     LYDEGDVIRLGDHEIQVLHTPGHSPGSVTLKVEDVLFTGDTLFAGSCGRTDLRGGSYE
                     QIMQSLKRLGELKGDFHVCPGHEATSTLERERRSNPFLMEAMRS"
     gene            1244..>2247
                     /locus_tag="ACZPAQ_07225"
     CDS             1244..>2247
                     /locus_tag="ACZPAQ_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR0903816.1"
                     /translation="MKLYLDLDNYDYQPERYKYAVEQMMLTLFPEERPEYPPREGRTL
                     KGETNAARFTLRRSPVWTTVRAQVYRPQGMGQAAVRVGSGELDRPPEQVYHTLQHALK
                     MAFYQAGTALLGGEPLWGALTGVRPVKLPTRAMLAGATEAQACRELEREYHVSPRRAR
                     LAVECARASLAEDRQLEEGQVSLYIGIPFCPTRCAYCSFVSADVGRALKLVDPYVEAL
                     LEEVRRTGQVLEQAGLRVRTLYVGGGTPTTLSASQLDRLLAAAEAHLPLAGCREMTVE
                     AGRPDTITREKLEVLREHGIERISINPQTMSDQVLQAIGRRHTARDIREAFCTAREVG
                     "
BASE COUNT          388 a          711 c          794 g          354 t
ORIGIN      
        1 tggtctccct gatcgtcatg gcagtgtgac gatcatcaaa aaacgatcga ggtgaaatgc
       61 gacgagagca gtggtgaccc gggtctcctc cgcctccgtc tccatcggcg ggacggtgga
      121 gggagccatt gagcagggct ttctggtcct gctgggggtg ggccccggag atacggaggc
      181 cgtgtgtgac aagctggcgg agaagatctg caacctgcgg gtctttgagg atgagaacgg
      241 caggatgaac ctgagcctgg agcaggtggg aggtgccctg ctggtggtgt cccagttcac
      301 cctgtatgcc gatacctcca gccgaagacc tggattcacc ggggcggcca gacccgacct
      361 ggcggtcccc ctgtacgagc gattcatgga gcagtgccgc cggcggggct tccaggtgga
      421 gcacgggacc ttcggcgcgg acatgcaggt ggcgtcggtg aacgacggac cagtgaccat
      481 tctgttcgat acggacaaaa acggagcggg aagggcgtaa agcgcggcgc ggtccgcgct
      541 ttggctgtcc ccgccggact ttgacccaaa agaggtgttc taacgatgaa agtaaagctg
      601 ctgcgggtgg ggcccatcgg caccaactgc tatattctgg aggacgacca gacgaacctg
      661 gccgccgtca tcgatcccgg cgacgagccg gagctgatcc aggaggcgct ggagaaggag
      721 ggggtggagg tccggtatct cctccttacc cacggccact atgaccacac cacggcggtg
      781 ccagccctcc accgggtgta tccccaggcg gatatctata tccaccaggc tgacgccaac
      841 ggggcgggca gtaccttgtt ccccctggcc ggcgaggtgg acgatctgaa gctctacgac
      901 gagggggatg tgatccgcct gggggaccac gagatccagg tactccacac ccccggccat
      961 tcccccggct ccgtcaccct gaaggtggag gacgtgcttt tcactgggga caccctgttc
     1021 gccggctcct gcggccgcac cgacctgcgg gggggcagct atgagcagat catgcagtcc
     1081 ctgaagcggc tgggggagct gaagggggac ttccacgtct gtcccggcca cgaggccacc
     1141 tccaccctgg agcgggagcg gaggagcaac cccttcctga tggaggccat gcggagctga
     1201 gggggcgcct gcccgaagat ttttcccgtg aggaaggtgg actatgaagc tgtatctgga
     1261 tctggacaac tatgactacc agccggagcg ctataaatac gcggtggagc agatgatgct
     1321 caccctcttc ccggaggagc ggccggaata tcccccccgg gagggccgta cactgaaggg
     1381 ggagaccaac gccgcccgct tcaccctgcg ccgcagcccc gtgtggacca ccgtccgcgc
     1441 ccaggtgtac cggccccagg ggatggggca ggctgcggtc cgtgtgggga gcggggagct
     1501 ggaccgtccg ccggagcagg tgtaccacac cctccagcac gccctgaaga tggcatttta
     1561 ccaggcgggc accgccctgc tggggggcga gccactctgg ggcgccctca ccggcgtcag
     1621 gccggtgaag ctgcccaccc gggccatgct ggcgggggcc acggaggccc aggcctgccg
     1681 ggagctggag cgggagtacc acgtctcccc ccgccgggcc cggctggcgg tggagtgcgc
     1741 ccgggccagt ctggcggagg accggcagct ggaagaggga caggtgtccc tgtatatcgg
     1801 catccccttc tgtcccaccc ggtgcgccta ctgctccttt gtctccgccg acgtgggtcg
     1861 ggccctgaag ctggtggacc cctacgtgga ggcgctgctg gaggaggtcc gccgcacggg
     1921 acaggtgctg gaacaggccg ggctgcgggt ccgcaccctg tatgtggggg gcgggactcc
     1981 caccaccctc tccgcatccc agctggaccg cctgctggcg gcggcggagg cccatcttcc
     2041 cctggcgggc tgccgggaga tgacggtgga ggcggggcgg ccggacacca ttacccggga
     2101 aaagctggag gtgctgcggg aacacgggat cgagcgcatc tccatcaatc cccagaccat
     2161 gtcggaccag gtgctccagg ccatcggccg ccgccacacc gcccgggaca tccgggaggc
     2221 gttctgcacc gcccgggagg tgggctt
//
LOCUS       DBMDSJ010000291         2242 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11219_length_2242_cov_1.702475, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000291 DBMDSJ010000000
VERSION     DBMDSJ010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11219_length_2242_cov_1.702475"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="ACZPAQ_07230"
     CDS             <1..231
                     /locus_tag="ACZPAQ_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR0903817.1"
                     /translation="GDLPLELQNPLLDLLDAPRRVGLCASASHILTPRKSVTAILGAA
                     DGPIQPTVRSCLGCPARESCQYRKSGGHCGIS"
     gene            218..>2242
                     /locus_tag="ACZPAQ_07235"
     CDS             218..>2242
                     /locus_tag="ACZPAQ_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR0903818.1"
                     /translation="MEFRELLNTPGPVLLDGGMGTMLQARGLSMGEWPELVALEHPDW
                     LEDIHRAYVEAGAQILCANTFGANREKLGRTGRTVEEVVPPSVEIARRAARGRALVAL
                     DVGPTGQLLEPTGTLRFEEAVDIFAQTVRAGVQAGADLILIETMTDLQEARAALLAAK
                     ENSSLPVIVTMTYEERGRTFLGCDPASAALTLEGMGADAIGVNCSLGPREMPPLVREL
                     TRWSTLPIAIKPNAGLPDPGGAGYDITAEEFAGSMAELTGLGVKLYGGCCGTTPEYIA
                     LLREKLAGCTIVQVPRQVPAAVCSGTRTVPIDRVRVIGERINPTGKKLMKEALRRGDV
                     DYMLNQALAQTEAGADILDVNVGLPEIDEAEMMVRTVKALQGVTDAPLQLDSTDPRVL
                     EGALRVYCGKAIVNSVNGDEETLNAILPLVKKYGAAVVGLTLDHTGIPKTVDARLSVA
                     RRILDRALSYGIRREDVYIDCLTLTVSAEQEAAAQTLEGLRRVKGELGLKTVLGVSNI
                     SFGLPARPLINQNFLTMAMAAGLDLPILNPNVDAMMAAVRCYHLLMNIDTDAREFIAA
                     YGNATVSTSITSAAGMTAAPAAGEKRGLEEIVEAGLKGEAGEATRALLESRAPLDVVD
                     NVLIPALDRVGAAFEAGKVFLPQLIQSAGAAQAAFEVIRERLSGQPGQGED"
BASE COUNT          366 a          749 c          797 g          330 t
ORIGIN      
        1 ggggacctgc ccctggagct ccagaacccc ctgctggacc tgctggacgc cccacggcgg
       61 gtggggctgt gcgccagcgc cagccacatc ctcacccccc gcaagtcggt gaccgctatc
      121 ttgggggcgg ccgacggacc catccaacca actgtgcgca gctgtctggg ctgtcccgcc
      181 cgggagagct gccagtatcg aaagtcaggt ggacactgtg gaatttcgtg aattgctgaa
      241 tacgccgggc cccgtcctgc tggacggcgg catgggcacc atgctgcagg ccaggggcct
      301 gtccatgggg gagtggcccg agctggtcgc cctggaacac ccggactggc tggaggacat
      361 ccaccgggcc tatgtggagg cgggggccca gatcctgtgc gccaacacct tcggcgccaa
      421 ccgggagaag ctcggccgca ccggccgcac cgtggaggag gtggtccctc cctcagtgga
      481 gatcgcccgc cgggcggcca gggggagggc cttagtggcc ctggacgtgg ggcccaccgg
      541 ccagctgctg gagcccactg gcaccctccg ctttgaggag gcggtggata tctttgccca
      601 gacggtgcgc gccggtgtcc aggccggggc ggacctgatt ctgatcgaga ccatgaccga
      661 cctccaggag gcccgggccg ccctgctggc cgctaaggag aacagcagcc tgcccgtcat
      721 cgtcaccatg acctatgagg agcggggccg caccttcctg ggctgcgacc cggcctctgc
      781 ggccctgacc ctggagggga tgggggcaga tgccatcgga gtgaactgct ccctgggccc
      841 ccgggagatg cccccgctgg tgcgggagct gacccggtgg tccaccctgc ctatcgccat
      901 caagcccaat gcgggcctgc ccgacccagg gggcgcgggg tatgacatta cggcggagga
      961 gtttgccggg agcatggcag agctgaccgg gctgggcgtt aagctgtacg gcggctgctg
     1021 cggcactacg ccggagtata tcgcactgct gcgggagaaa ctggcgggct gcaccatcgt
     1081 ccaggtgccc cggcaggtgc ccgcggcggt gtgcagcggc acccgcaccg tgcccatcga
     1141 ccgggtgcgg gtcatcgggg agcggatcaa tcccaccggc aaaaagctga tgaaagaggc
     1201 cctgcgccgg ggggatgtgg actacatgct gaaccaggcc ctggcccaga ccgaggcggg
     1261 ggccgacatc ctggatgtga acgtgggtct gcccgagatc gacgaggcgg agatgatggt
     1321 gcgcacggtg aaggccctcc agggggtcac cgacgccccc ctccagttgg actccaccga
     1381 cccccgggtg ctggagggag ccctgcgggt ctactgcggc aaggccattg tcaactcggt
     1441 gaacggggac gaggagaccc tgaacgccat cctccccctg gtgaagaagt acggcgctgc
     1501 ggtggtgggc ttgacccttg accacaccgg catcccaaag acggtagacg cccggctctc
     1561 ggtggcccgc cgtatcctgg accgggccct gtcctatggc atccggcggg aggatgtgta
     1621 catcgactgc ctgaccctca ccgtctccgc tgagcaggaa gccgccgccc agaccctgga
     1681 gggcctgcgg cgggtgaagg gagagctggg cctgaagacg gtgctggggg tgtccaacat
     1741 ctccttcgga ctgcccgccc ggcccctgat caaccagaat ttccttacca tggccatggc
     1801 cgccggcctg gacctgccca tcctcaaccc caatgtggac gctatgatgg cggcagtgcg
     1861 gtgctaccac ctgctgatga acatcgacac cgacgcccgg gaattcatcg ccgcttacgg
     1921 gaacgccacc gtctccacct ctatcaccag cgcggccggg atgaccgccg cccccgcagc
     1981 gggggaaaag cggggcctgg aggagatcgt ggaggccggc ctgaaggggg aggcggggga
     2041 ggccacccgg gccctgctgg agagtcgggc ccccctggac gtggtggaca acgtcctcat
     2101 ccccgccctg gaccgagtag gggcggcctt cgaggcggga aaggtgttcc tgccccagct
     2161 gatccagtct gcaggggcgg cccaggccgc cttcgaggtc atccgggagc gcctgtccgg
     2221 ccagccgggc cagggggagg ac
//
LOCUS       DBMDSJ010000292         2226 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11295_length_2226_cov_1.729412, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000292 DBMDSJ010000000
VERSION     DBMDSJ010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11295_length_2226_cov_1.729412"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="ACZPAQ_07240"
     CDS             <1..1242
                     /locus_tag="ACZPAQ_07240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR0903819.1"
                     /translation="IIGGTRFFDRAEVKDMIAYLAVLNNPEDDLRLTRIINNPPRGIG
                     AKTVETAQEIARREGVSLFAVIDNARMYPELDRAAAKLAQFSGMMGELAQQAQTMPLP
                     DFYEELIARTGYAVMLESKNTVEDRTRLENVRELLTSINGYLENAGEEPSLAGFLDEI
                     ALYTDLDSHDPDQDCVVMMTMHSAKGLEFPVVFVVGAEEGIFPGIRAIGETEEMEEER
                     RLCYVAMTRAKEKLYLTCANQRMLFGRTSSNRPSRFVDEIPTEHLERSGRTFLDPGVG
                     DWGGIPSRTSGYGGYQRGEQSYGGYAARGSEAPQRPAPPSYGGFTRAMGGRTGGVYPA
                     APAAGRPQRPLGCRPAAAAVPAALPTFQKGDMVRHKAFGQGMILSVQKMGGDALVEIA
                     FDNVGTKRLMLKSAAAHMEKI"
     gene            1255..1743
                     /locus_tag="ACZPAQ_07245"
     CDS             1255..1743
                     /locus_tag="ACZPAQ_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903820.1"
                     /translation="MDRNKRTARTLTAALCLALLAGCGAETAGESLDADEMHPGWSTV
                     TTDPSAYGITYQELEDLSGMPLDQLTAYCLGADGVYGENGFDQLYHRFLEAPDTVLSY
                     LALIDSREERELLCTHLASADVSWYGGTEAFGAILDRLAGESGTSGELAELIQTEWKS
                     LQ"
     gene            complement(1819..>2226)
                     /locus_tag="ACZPAQ_07250"
     CDS             complement(1819..>2226)
                     /locus_tag="ACZPAQ_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903821.1"
                     /translation="ALLCGIALFLGRSAIPHLFGASNAATRLFQVILPLFLLYLPFMS
                     VVRILTSYFCATEQITRADLLIYGQPVVLVALSLILPRTGLGLSGVWLSVPLTQILLA
                     GAGVLLLHTAHAGTKPIPAAAPQQAVPSVSLSV"
BASE COUNT          417 a          681 c          807 g          321 t
ORIGIN      
        1 atcatcgggg gcacccgctt cttcgaccgg gcggaggtca aggacatgat cgcctatctg
       61 gcggtgctga acaacccgga ggacgacctg cgcctcacta ggatcatcaa caaccctccc
      121 cgtggcatcg gggccaagac ggtggagacc gcccaggaga tcgcccggcg ggagggggtg
      181 tccctctttg ccgtcatcga caacgcgcgg atgtaccccg aactggaccg ggcggcggct
      241 aagctggccc agttctccgg aatgatgggg gagctggccc agcaggccca gaccatgccc
      301 ctgcccgact tctatgagga gctcatcgcc cgcaccggtt acgccgtgat gctggagagc
      361 aagaacaccg tggaggaccg gacccgtctg gaaaacgtgc gggagctgct cacctccatc
      421 aacggctatc tggagaatgc gggggaggag ccctccctgg cgggcttcct ggatgagatc
      481 gccctgtaca ccgacctgga cagccacgac ccagatcagg actgcgtggt gatgatgacc
      541 atgcactcgg ccaaggggct ggagttcccg gtggtctttg tggtgggggc cgaggagggc
      601 atcttccccg gtatccgggc catcggagag acggaggaga tggaggagga gcgccgcctg
      661 tgctacgtgg ccatgacccg tgccaaggag aagctgtacc tcacctgcgc caatcagcgc
      721 atgctgttcg gccgcaccag ctccaaccgt cccagccgct ttgtggacga gatccccacg
      781 gagcatctgg agcgctccgg ccgcaccttc ctggacccgg gcgtggggga ctgggggggc
      841 atccccagcc gcacctccgg gtacggggga taccagcggg gggagcagag ctacggcggc
      901 tacgcggccc gggggagcga ggcgccccag cggcccgctc cgccttccta cggcggtttc
      961 acccgggcca tgggggggcg caccggcggc gtctatcccg ccgcgcccgc ggccggccgt
     1021 ccccagcgcc ccctgggctg ccgcccggct gccgccgcag taccagcggc gctgcccacc
     1081 ttccagaagg gggacatggt gcgccacaag gcattcggac aggggatgat cctgtccgtg
     1141 cagaagatgg ggggcgatgc cctggtggag atcgcctttg acaacgtggg caccaagcgg
     1201 ctgatgctca aatccgctgc cgcccacatg gagaagatat gaggaggagc ggctgtggac
     1261 cgaaataaaa ggacggccag gacgctgacg gcggcgctct gcctggccct tctggctgga
     1321 tgcggcgcgg aaacggccgg ggagagcctg gatgccgatg agatgcaccc gggatggagc
     1381 accgtgacca ccgatccctc cgcctacggc atcacctatc aggagctgga ggatctgagc
     1441 ggcatgcccc tggaccagct gacggcctac tgcctggggg cggacggggt ctacggggag
     1501 aacggctttg accagctgta ccaccggttc ctggaggccc cggataccgt gctgtcctac
     1561 ctagctttga tcgacagccg ggaggagcgg gagctgttgt gcacacactt ggcttccgct
     1621 gatgtgtcct ggtacggcgg gaccgaggcg tttggagcga ttttggaccg cctggccgga
     1681 gagagcggga ccagtgggga gctggcggag ctgatccaga cagagtggaa atctttgcaa
     1741 taaacagaaa tagctgcgcg ccctcccgaa aggagggcgc gcagccgttt tttggataag
     1801 ggcccgtggg catccatgtc acacagacag agagacggag gggacggcct gctggggcgc
     1861 ggcggcgggg atgggcttcg tcccggcgtg agcggtgtgc agcagcagca ccccggcgcc
     1921 ggccagcagg atctgggtca gggggacgga cagccagacc ccggacagcc ccagcccagt
     1981 gcggggcagg atcagggaga gggccaccag cacaacgggc tggccgtaga tcagcaggtc
     2041 ggcccgggtg atctgctcgg tggcgcagaa gtaggaggtc aggatgcgca ctaccgacat
     2101 gaacgggaga tagagcagga acaggggcag gatcacctgg aacagccggg tagcggcatt
     2161 ggatgcccca aacaggtggg ggatggcgga ccgccccaga aagagggcga tgccgcagag
     2221 cagggc
//
LOCUS       DBMDSJ010000293         2224 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11306_length_2224_cov_1.280735, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000293 DBMDSJ010000000
VERSION     DBMDSJ010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11306_length_2224_cov_1.280735"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="ACZPAQ_07255"
     CDS             complement(<1..449)
                     /locus_tag="ACZPAQ_07255"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR0903822.1"
                     /translation="MVKAIVGANWGDEGKGKITDMLAEQSDVVIRFQGGANAGHTIIN
                     DYGRFALHILPSGTFYPHVTNIIGNGVALDIQKLVDELGSITSQGVPAPNLIVSERAQ
                     ILMPYHRLQDSYEEERLGGKAFGSTKSGIAPFYSDKYAKIGIQVCDL"
     gene            811..>2224
                     /locus_tag="ACZPAQ_07260"
     CDS             811..>2224
                     /locus_tag="ACZPAQ_07260"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR0903823.1"
                     /translation="MRAMTRRALALVLTLAMALSLCLAAQAAGPQVTVTLDGTALELE
                     VPAYIDGQDRTQVPASIGPQLGLTVQTGDGSVTFTKGEASLTFQDGAKEAGGVTMDTA
                     ADLSGEVGYVLLTYLAQFFGFQVAWNGVTRTAAVTSTAAEEETAVTKLPQLVYSELES
                     LMAQQAAQYQTKDNVVPMLWQGVLEMDIQVGDAARTAKLYVPQGTPQGAMFVVMNVPE
                     GLNAVSFMETSGWMDKADEEGFCLFVLEPAQGTPWGTLEEEQAYINAGISAAKAGKWL
                     QPGPSMYLVGYGAMGTCLQKYAMENPLNIAGAVFLDASLIDAGYMASNGDVSFDTDTK
                     TYGVTRKEVPVPVKIVESTMTEQAKAVADYWQAAANDKNAVERFAPEGGEILAAAVVT
                     EEGNYDYALPATTDMIWDFMGQFYRYGGGVLSNAISWKVDYEEMGVEFRSFTDSQGID
                     RQYLVYIPEAYRGSGEQLPVV"
BASE COUNT          445 a          644 c          735 g          400 t
ORIGIN      
        1 aacaggtcgc agacctggat gccgatcttg gcgtatttgt ccgagtagaa gggggcgatg
       61 ccgctcttgg tggagccaaa ggccttgccg cccaggcgct cctcctcgta gctgtcctgg
      121 agacggtggt agggcatcag gatctgggcc cgctccgaga cgatcaggtt gggggcgggg
      181 acgccctggc tggtgatgga gcccagctcg tccaccagct tctggatatc cagtgccacg
      241 ccgttgccga tgatgttggt cacatgggga tagaaggtgc cggaggggag gatgtgcagg
      301 gcgaaccggc catagtcgtt gatgatggta tggcccgcgt tggccccgcc ctgaaagcgg
      361 atgaccacgt cggactgctc tgccagcatg tccgtgatct tgcccttgcc ctcgtcgccc
      421 cagttggcgc ctacgatcgc ttttaccata tctgttacct ccgtggaccg ggattcgcaa
      481 ccgcacagcg gatttgcctt atccggccaa catgacagca ttcttattat acttcctcta
      541 aaagagggat gcaagggaaa aagctgtttt tcccatgtat ttttccgaac aacggcagaa
      601 atggagccca tgagtctgcg gagacgatag ggcgggagaa cattgacaga atggtaaaaa
      661 aacattagaa taaaaaagcg ccttttttgg tcggaatgga caaagctctg cggtgagccg
      721 gcgcggagta gaagaaattt tccccggaac aggcgctcag acaccccgga ctgcacatgc
      781 ggtccgggag aaatagggag gtagcagagt atgagagcaa tgacaagacg cgcgctggcg
      841 ctggtgctga ccctggctat ggcgctgtcc ctgtgtctgg cggcccaggc cgccggcccc
      901 caggtgacgg tgaccctgga tgggacggcg ctggagctgg aggtccccgc ctatatcgac
      961 gggcaggacc ggacccaggt ccccgccagc atcggccccc agctggggct gacggtccag
     1021 actggtgacg ggagcgtcac cttcaccaaa ggagaggcca gcctgacctt ccaggacggg
     1081 gccaaggagg cgggcggcgt gaccatggac accgccgccg acctgtccgg cgaggtgggc
     1141 tatgtgctcc tgacctatct ggcccagttc ttcggcttcc aggtggcctg gaacggcgtc
     1201 acccgcactg cggcggtcac tagcacggcc gcggaggagg agaccgctgt gaccaagctg
     1261 ccccagctgg tgtattcaga gctggagagc ctgatggccc agcaggcagc ccagtatcag
     1321 accaaggaca acgtggtgcc catgctgtgg cagggcgtgc tggaaatgga catccaggtg
     1381 ggcgatgccg cccgcaccgc caagctctac gtgccccagg gcacgcccca gggcgccatg
     1441 ttcgtggtga tgaacgtccc tgagggcctg aacgccgtga gcttcatgga gaccagcggc
     1501 tggatggaca aggcggacga ggagggtttt tgcctgttcg tgctggaacc cgcccagggt
     1561 actccctggg gaactctgga ggaggagcag gcatacatca atgccggcat cagcgccgcc
     1621 aaggcgggca agtggctcca gcccggtccc agcatgtacc tggtgggcta cggtgccatg
     1681 ggtacctgcc tgcagaagta cgccatggag aatcccctga atatcgccgg agcggtgttc
     1741 ctggatgcca gcctcattga cgccggctac atggcgtcca acggggatgt gtcctttgat
     1801 accgatacca agacctacgg cgtcacccgc aaggaggtgc cggtgcccgt caagatcgtg
     1861 gaaagcacca tgacggagca ggcaaaggct gtggcggact actggcaggc cgcagccaac
     1921 gacaagaacg ccgtggagcg ctttgctcct gaaggcggcg agatcctggc ggccgccgtg
     1981 gtcaccgaag aggggaacta tgactacgcc ctgcctgcca ccaccgacat gatctgggac
     2041 ttcatgggcc agttctaccg ctacggcggc ggtgtgctgt ccaacgctat ctcctggaag
     2101 gtggactatg aggagatggg agtggagttc cgaagcttca ccgactccca gggcatcgac
     2161 cggcagtatc tggtctatat ccctgaggct taccgcggca gcggcgagca gctgcccgtg
     2221 gtca
//
LOCUS       DBMDSJ010000294         2221 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11319_length_2221_cov_1.512736, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000294 DBMDSJ010000000
VERSION     DBMDSJ010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11319_length_2221_cov_1.512736"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="ACZPAQ_07265"
     CDS             <1..369
                     /locus_tag="ACZPAQ_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR0903824.1"
                     /translation="PAVAGGESSFWPAFAAEAKRKLPSAGPYLVNPEKTVGTWKNGVL
                     TLWVDSAFTKAMLNKPNITEVLAQAAGQRFGGQARVAFQIGKPSPEDLGTGQSPEAGQ
                     RDALDALLEFGEQFDNIIIK"
     gene            402..746
                     /locus_tag="ACZPAQ_07270"
     CDS             402..746
                     /locus_tag="ACZPAQ_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR0903825.1"
                     /translation="MAKGFGGRGGMGGMGMNANMLKQAQKMQQDMMRMQQELQEKEYE
                     AAAGGGVVTATVTGKRELKALEIDPEAVDPDDVEMLQDMIVAAVNEALRAAENDAAST
                     MQKLTGGLGLPF"
     gene            868..>2221
                     /locus_tag="ACZPAQ_07275"
     CDS             868..>2221
                     /locus_tag="ACZPAQ_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0903826.1"
                     /translation="MKRESPEEQRKQMLEAPIPGLIGRLAVPTIISMLVTSIYNMADT
                     YFVSHIGAGDGATSASGAVSIVFSLMAIIQAVGFTVGMGAGSITSRLLGKGWKERADQ
                     YASSAVILGLVLGLLLMSVCFVWIDELVWLLGATPTIYPYALDYARYIIFGAPVMILA
                     YILNNLLRWQGKANRSVIGLGFGGLLNIVLDPILIFTFDMGISGAAIATLFSQCVSLA
                     ILASFFVRGQSDIRVSPRLIAREAKVYGDILKSGLPSFLRQGMSSVASVSLNLNAGLY
                     GDPAVAAMGIVTKVFTFIMSVVIGFGQGFQPVVGFNYGAGRLDRVRESVKFSMKSCTL
                     VLTAAAVIGFLFAPQVIHFFREDTEVIAIGARAFRFQCVSLPLGAVLVFANMLFQSLG
                     CSWRASFLAVCRQGLFFIPLIFLLPRLFDLTGLEMTQMVADFITFLCSGGMLLHFFRT
                     E"
BASE COUNT          406 a          635 c          757 g          423 t
ORIGIN      
        1 ccggccgtcg ctgggggcga gagctccttc tggcccgcct tcgcggcgga ggccaagcga
       61 aagctgccct ccgcggggcc ctatctggtg aacccggaaa agacggtggg gacctggaaa
      121 aacggggtgc tgaccctgtg ggtggacagc gcattcacca aggcgatgct caacaagccc
      181 aacatcacgg aggtgctggc acaggccgct gggcagcggt tcggcgggca ggccagagta
      241 gccttccaaa tcgggaagcc ctctccggag gacctgggca cggggcagtc cccggaagct
      301 ggacagcggg acgccctgga cgccctgctg gagtttgggg aacagtttga caatatcatc
      361 atcaagtgat atggttatat cggaataagg aggggatgga tatggcaaag ggctttggag
      421 gccgcggcgg catgggcggc atgggaatga acgccaacat gctcaagcag gcccagaaga
      481 tgcagcagga tatgatgcgc atgcagcagg agctccagga gaaggagtac gaggccgccg
      541 ccggcggcgg ggtggtgact gccactgtga ccggcaagcg ggagctgaag gcgctggaga
      601 tcgatcctga ggcggtggac ccggatgacg tggagatgct ccaggacatg atcgtggccg
      661 ccgtcaacga ggcgctgcgg gctgcggaaa acgatgcggc ttccaccatg cagaaactga
      721 ccggcgggct cggactgccg ttttaagagg aacgacgccc catccccccg gaagagaccg
      781 gagggatggg gcgctttttt accgctggcg ggcggcggag agaggaaggc agggagagaa
      841 gccgggaaag gcggaaagga gaaaaaagtg aagcgggaaa gtccggaaga acagcggaaa
      901 cagatgctgg aggcccccat cccgggactg atcggcaggc tggcggtgcc caccatcata
      961 agcatgctgg tgacctccat ctacaacatg gcggacacct attttgtctc ccacatcggc
     1021 gcgggggatg gagccaccag cgcctccgga gcggtgagca tcgtcttttc cctcatggcg
     1081 atcatccagg cggtgggctt caccgtgggg atgggggcgg ggagcatcac ctcccggctg
     1141 ctggggaagg gatggaaaga gcgggcggac cagtacgcat ccagcgccgt cattctggga
     1201 ctggtgctgg ggctgctgct catgtccgtc tgcttcgtct ggatcgatga gctggtctgg
     1261 ctgctggggg ccacccccac catctatccc tacgccctag actacgccag gtatatcatc
     1321 ttcggtgccc cggtgatgat cctggcctat atcctcaaca atttgctgcg gtggcaggga
     1381 aaggccaacc ggtcggtgat cggcctggga ttcggcggac tgctgaacat cgtgttggac
     1441 cccatcctga tcttcacctt tgacatgggc atctccgggg cggccatcgc caccctgttc
     1501 agccagtgcg tcagcctggc tatcctggcc tccttctttg tgcgagggca gagcgacatc
     1561 cgggtcagcc cccggctcat cgctcgagag gccaaggtgt atggagacat cctgaaaagc
     1621 ggtctgccat ccttcctgcg gcagggcatg tccagtgtgg cctccgtgtc tctgaacctc
     1681 aatgcggggc tctacggcga cccggcggtg gccgccatgg ggatcgtgac caaggtgttt
     1741 accttcatca tgtccgtcgt catcggcttc ggccaggggt ttcagcctgt agtaggcttc
     1801 aactacggtg cggggcggct ggaccgggtg cgggagagcg tgaagttctc catgaagtcc
     1861 tgcaccttgg tgctgactgc ggcggccgtc attgggtttc ttttcgcacc ccaggtgatc
     1921 cacttcttcc gggaggacac ggaggtgatc gccatcgggg cccgggcttt ccggttccag
     1981 tgtgtcagcc ttcccctggg ggcggtgctg gtctttgcca acatgctctt ccagtcgctg
     2041 gggtgctcct ggagggcctc cttcctggcc gtatgccggc aggggctgtt ctttatccct
     2101 ctgatcttcc tgctgcccag gctgtttgat ctcaccgggc tggagatgac ccagatggta
     2161 gcggacttca tcaccttcct gtgcagcgga gggatgctgc tccacttctt ccgtacggag
     2221 c
//
LOCUS       DBMDSJ010000295         2220 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11327_length_2220_cov_1.109014, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000295 DBMDSJ010000000
VERSION     DBMDSJ010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11327_length_2220_cov_1.109014"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(2..1285)
                     /gene="yfmH"
                     /locus_tag="ACZPAQ_07280"
     CDS             complement(2..1285)
                     /gene="yfmH"
                     /locus_tag="ACZPAQ_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P 5-aminopentanol modification-associated
                     protein YfmH"
                     /protein_id="HIR0903827.1"
                     /translation="MEKLEFSRVGERMYHEKLANGLNVFVFPKPEFQKGYAFFATNYG
                     GMDMKFCLDGQWHQSPAGVAHFLEHKMFDTKEGNALQDLAANGASPNAFTSSAITGYY
                     FESTEKFEENLRILLSFVSVPWFTQESVDKEQGIIGQEIGMIEDDPDWKVFMNLMAAL
                     YQHHPIRVSVAGSVESISHITADTLYACHKAFYDPANMVLCVAGPVEAEHICQVAREI
                     LPRQAGPIAAKDYGEKEADRVAQSMVEERMEVSTPIFQLGYKGDAPQRGESGLRQELV
                     AELALEGLLGNSTPLYSKLYREGLINQNFSYGYESYPGCSFLCAGGESKDPEAVRMAV
                     AEEAGRIGREGIDGKLWERLKKGVYGNKVRGLNSFENLCVGQARSFFAGVNFLQFADL
                     FSSITKEEAEDLIGRWVTEERAALSVVRPREGGQA"
     gene            complement(1289..>2220)
                     /locus_tag="ACZPAQ_07285"
     CDS             complement(1289..>2220)
                     /locus_tag="ACZPAQ_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR0903828.1"
                     /translation="LLSPYTQDGCFCSEYTDQEKNNLTDRIRSQINDKRAYATYRLTQ
                     LMCGEEAFGVDKLGDEESVAAVTPDSLWRRYQELLKGAAIEVYYCGSAQPERAEGALR
                     TALAALPISGERLDPDCEVRITAGPQPQVVEEAMDVSQGKLALGFRTGGVTCWEEDYP
                     ALTMFNAVFGGTTLSKLFMNVREKLSLCYYASSMLEKMKGLVLVSSGIEFDKYQTAKD
                     EILSQLEAIRRGEIQDWELEGARRTLIGGHLATLDDQGRQEEFWLGQAAAGLDTTIPE
                     LTAQFEEVTREQVSAVARKLELDTVYFLKSKEG"
BASE COUNT          409 a          740 c          642 g          429 t
ORIGIN      
        1 atcaagcctg ccccccctct ctgggacgga ccacagacag ggccgcgcgc tcctccgtga
       61 cccagcggcc gatcaggtcc tccgcctcct ccttggtgat ggagctgaac aagtccgcaa
      121 attggaggaa gttcaccccg gcaaagaagg atcgagcctg acccacacac aggttttcga
      181 aggaattgag gccccggacc ttgttgccgt agacgccctt cttcaaccgc tcccaaagct
      241 tgccgtcaat gccctcccgg ccgatgcgac cggcctcctc agccaccgcc attcggaccg
      301 cctccgggtc cttggactcc cccccggcgc acaggaagga acagccagga taggactcat
      361 acccgtagga gaaattctga ttgatgagcc cctcccggta cagcttggaa tacagcggag
      421 tggagttgcc caacaggccc tccagggcca gctcggccac cagctcctga cgcagaccac
      481 tctcgcccct ctggggggcg tcgcccttgt agcccagttg gaagatggga gtggacacct
      541 ccatccgctc ctccaccatg gattgggcca cacggtcggc ctccttctcc ccatagtcct
      601 tggcagcaat tgggcccgcc tgcctgggca ggatctcccg ggccacctgg cagatatgct
      661 ccgcctccac agggcccgcc acgcacagca ccatgttggc cggatcgtag aaggccttgt
      721 ggcaggcgta cagggtgtcg gcggtgatgt gggagatgga ctccacactg cccgccacgc
      781 tcacccggat ggggtgatgc tggtacaggg cggccatcag gttcataaag accttccagt
      841 ccgggtcgtc ctcgatcatg ccgatctcct ggccgatgat gccttgttcc ttgtctacgc
      901 tctcctgagt aaaccagggg acagaaacaa aggagaggag gatgcgcagg ttctcctcaa
      961 atttctccgt ggactcgaag tagtagcccg tgatggcgga gctggtaaag gcgttggggg
     1021 aggccccgtt ggccgccagg tcctggaggg cgttgccctc cttggtatcg aacatcttgt
     1081 gctcaaggaa gtgggccaca ccggccgggc tctgatgcca ctggccgtcc agacagaatt
     1141 tcatgtccat gccgccgtag ttggtggcaa agaaggcata ccccttctga aattccggct
     1201 ttgggaatac gaacacattg agtccgttgg ccagcttttc gtggtacatc cgctccccta
     1261 cccgggaaaa ttccagcttt tccatggctc aaccctcctt gctcttcagg aaatatacag
     1321 tgtccagctc cagcttccgg gccacggcgg acacctgctc ccgtgtgacc tcctcaaact
     1381 gggcggtcag ctccgggatg gtggtatcca ggccagcggc ggcctggccc agccagaact
     1441 cctcctgccg gccctggtcg tccaacgtgg ccaagtggcc gccgatcagg gtgcgcctgg
     1501 ctccctccag ctcccagtcc tggatctcac cccggcggat ggcctccagt tgggacagga
     1561 tctcgtcctt tgccgtctgg tatttgtcaa actcgatacc agaggatacc aggaccagcc
     1621 ccttcatctt ctccagcata gagctggcgt agtagcacag ggacagcttc tcccggacgt
     1681 tcataaagag cttggacaga gtagtcccgc caaagacggc attgaacatg gtcagggccg
     1741 ggtagtcctc ctcccagcag gtaacgcctc cggtgcggaa gcccagggcc agcttgccct
     1801 ggctcacgtc catggcctcc tccaccacct ggggctgggg tccggccgtg atgcgtacct
     1861 cgcaatccgg gtccaggcgc tctccgctta tcggaagggc agccagagcg gtacgcaggg
     1921 ccccctccgc ccgctcgggc tgggcggagc cgcagtagta cacctcaatg gccgccccct
     1981 tcagcagctc ctgatagcgc cgccaaaggg agtcgggggt gacggccgct acgctctcct
     2041 catcccccag cttgtccacc ccaaaggcct cctctccgca cataagctga gtaaggcggt
     2101 aagtggcata ggcccgcttg tcattgatct ggctccggat ccggtcggtc aggttgttct
     2161 tctcctggtc cgtatactcg gagcagaagc agccgtcctg ggtgtaggga gagagcagca
//
LOCUS       DBMDSJ010000296         2216 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11357_length_2216_cov_1.651537, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000296 DBMDSJ010000000
VERSION     DBMDSJ010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11357_length_2216_cov_1.651537"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="ACZPAQ_07290"
     CDS             complement(<1..1639)
                     /locus_tag="ACZPAQ_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR0903829.1"
                     /translation="MAQKKPSYGNESISALKGADRVRKRPGVIFGSDGLEGCEHAVFE
                     IMSNAIDEAREGHGDLIIVTRFEDKSIQVEDFGRGCPVDWNEKEQRYNWELVFCELYA
                     GGKYGGDGDNYEYSLGLNGLGSCATQYASEYFDATIYRDGSKYTLHFEKGENIGGLHK
                     EPTDRGRKTGSTFRWKPDLEVFTDIDIPVEYYLDVMKRQAVVNSGVTFRFRNQTGGKF
                     ETTDFRYDNGILDYVTELAGENPLTQPVFWQTERKGRDREDKPEYKVKLSVSFCFSNR
                     VSIVEHYHNSSWLEHGGSPEKAMKSAFVSAIDARLKQLGKYQKSEGKISFNDIQDCLV
                     LVSNNFSTQTSYENQTKKAINNKFVQEAMTDFLRAQLEVYFIENPFDADKICEQVLIN
                     KRSRETAEKTRLNIKKKLSGSMDISNRVQKFVDCRTKDVSRRELYIVEGDSAMGAVKL
                     SRDAEYQGIMPVRGKILNCLKADYAKIFKNEIITDLLKVMGCGVEVQDKHVKDLASFD
                     LDSLRWNKVVICTDADVDGFQIRTLILTMLYRLTPTLI"
     gene            1855..>2216
                     /locus_tag="ACZPAQ_07295"
     CDS             1855..>2216
                     /locus_tag="ACZPAQ_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903830.1"
                     /translation="MENFWVAEESEGIGEVVRRQLGERDRRTAAGGSPWLLVVSPRAA
                     ERRRQLPTGCRTVLLPGGAGGLLSGLDPAAAVSYGTGPRDTLTLSSREGRQLWCAIQR
                     ELVTVEGRVVERQELVLP"
BASE COUNT          400 a          618 c          753 g          445 t
ORIGIN      
        1 ggatcagggt gggggttaga cggtagagca tggtgaggat cagggtgcgg atctggaagc
       61 cgtccacgtc ggcatcggtg cagatgacca ccttgttcca gcgcaggctg tccaggtcaa
      121 aggaggccag gtccttcacg tgcttgtcct ggacctccac tccgcagccc atgaccttga
      181 gcaggtcggt gatgatctcg tttttgaaga tcttggcgta atccgccttg aggcagttga
      241 ggatcttgcc ccggacgggc atgatgccct ggtactccgc gtcccggctg agcttcaccg
      301 cccccatggc ggagtctccc tccacgatgt agagctcccg gcgggacacg tccttggtgc
      361 ggcagtccac gaacttctgc acccggttgg agatgtccat ggagccggac agcttctttt
      421 tgatgttcag gcgggtcttt tccgcggtct cccggctacg cttgttgatg agcacctgct
      481 cgcagatctt gtctgcgtcg aaggggttct cgatgaaata gacctccagc tgggcgcgga
      541 ggaaatcggt catagcctcc tggacgaatt tgttgttgat ggccttttta gtctggttct
      601 cgtagctggt ctgggtggag aagttgttgg agaccagcac caggcagtcc tggatgtcgt
      661 tgaagctgat cttgccctcc gacttctggt acttgcccag ctgcttgagc cgggcgtcga
      721 tggcggagac gaaggcggat ttcatggcct tctccgggct gcccccgtgc tccagccagg
      781 aggagttgtg gtagtgctcc acgatggaca cccggttgga gaagcagaag gagaccgaca
      841 gcttcacctt gtactccggc ttgtcctccc ggtcccggcc cttccgctcg gtctgccaaa
      901 acaccggctg ggtcagggga ttttccccag ccagttcagt gacatagtcc aggatgccgt
      961 tgtcatagcg gaagtcggtg gtctcgaact tcccaccggt ctggttgcgg aagcggaagg
     1021 tgacgccgga gttgaccaca gcctggcgct tcatcacgtc caggtagtac tccacgggga
     1081 tgtcgatgtc ggtaaagacc tccaggtcgg gcttccagcg gaaggtggag cccgtctttc
     1141 tgccccggtc agtgggctct ttgtggagcc cgccgatatt ctcccccttc tcaaagtgga
     1201 gggtgtattt ggagccgtcc cggtagatgg ttgcgtcgaa atactcgctg gcgtactggg
     1261 tggcgcagga gcccaggccg ttgaggccca gggaatactc atagttgtcg ccgtccccgc
     1321 cgtacttgcc gccggcgtac agctcacaga acaccagctc ccagttgtag cgctgctcct
     1381 tttcgttcca gtccaccgga cagccccggc cgaagtcctc cacctggatg gacttgtcct
     1441 cgaaccgggt gacgatgatg agatcgccgt ggccctcccg ggcctcgtcg atggcgttgg
     1501 acatgatctc aaacacggca tgctcgcagc cctccagccc gtcggagccg aagatgacgc
     1561 cggggcgctt gcggacccgg tcggcccctt ttagggcgga gatggattcg ttgccgtagc
     1621 tgggtttctt ctgagccata gtaataaaga tccctcacag tacaatgcgg cgcgggaccg
     1681 cgcaaaataa caattataat aataccccat ttggacggag aaagtcaaga aaagggcgga
     1741 gcggcctggg acagaaaaaa gaggctggga gacagacaaa aagggaaaga atgggtgggg
     1801 cgggaataaa aagaggggaa atgccccaga ctgaggagga aaggggcggg atctgtggag
     1861 aatttctggg tagccgagga gagcgagggc atcggggagg tggtgcgccg gcagctgggg
     1921 gagcgggacc ggcgcacggc agcgggagga tcaccctggc tgctggtggt ctccccccgg
     1981 gcagcggagc ggaggaggca gctgcccact gggtgccgga cggtgcttct gcccggcgga
     2041 gcgggggggc tgctctccgg gctggacccc gcggcggcgg taagctacgg caccgggccc
     2101 agggacaccc tgaccctgtc cagccgggag gggcggcagc tgtggtgcgc catccagcgg
     2161 gagctggtga cggtggaggg acgggtggtg gagcggcagg agctggtgct cccctt
//
LOCUS       DBMDSJ010000297         2216 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11358_length_2216_cov_1.635934, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000297 DBMDSJ010000000
VERSION     DBMDSJ010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11358_length_2216_cov_1.635934"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="ACZPAQ_07300"
     CDS             <1..515
                     /locus_tag="ACZPAQ_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0903831.1"
                     /translation="AGLTYRMGYSYGGTDTLSKILKQKVFKAAELKNLLLCLDAVIML
                     IMLSAFSLDTVAYAFVGQIIYVNSMNYVIFNMGPRLYEIQIVCEDPRAIERFIIEEIG
                     KSATISKVIGGYSQADKIQLDCVCNSREYVQLREFIRDSGIDCFIKVFPLTHVFGRSK
                     DFHRLSDQNL"
     gene            649..927
                     /locus_tag="ACZPAQ_07305"
     CDS             649..927
                     /locus_tag="ACZPAQ_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903832.1"
                     /translation="MEQELFLPTLMHWQYGNNWCGSLKRASFWITPKDEQLTAQLWTG
                     PAIRENAEILYTAQFPISDEGLEQLRTWLLEHAAAVNAANPESFRYAW"
     gene            978..1874
                     /locus_tag="ACZPAQ_07310"
     CDS             978..1874
                     /locus_tag="ACZPAQ_07310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0903833.1"
                     /translation="MNLNQLYYLQAIAQARGLTRAADALYVTQSNLSHSMAALEEELG
                     IPLLYKNGRDTLLTPYGQEFLAYAERAIQEIEEGKRVAQSRCSPTRGQVRLGFISAVS
                     ATLIPWCVSHFYQNPDNQGITFTFDEKPTRRIAADFTHHALDIGFGTRFDDSSFEFCP
                     VVPEELVAVVPAGHPLAQQEQVTLEQLSRERLVIYNQASPTRNLVLSMFRQQDLQPDV
                     AFEVATDHMLASFVSQGLGVGIMPRMFGLRLYSVRPLYIADTETHRMLYMFRPKDRRV
                     LPAVQRFWDFVLEVCAQYQSDE"
     gene            complement(1939..>2216)
                     /locus_tag="ACZPAQ_07315"
     CDS             complement(1939..>2216)
                     /locus_tag="ACZPAQ_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR0903834.1"
                     /translation="SQILVEAWSPLGRQRLADDPLLAELAGKYGVSQAQICLRFALDC
                     GVVPLPKSADAGRMRQNLALDFALEAEDLERLRTMPQTGWSGLHPDK"
BASE COUNT          412 a          752 c          633 g          419 t
ORIGIN      
        1 gcgcaggcct gacctaccgc atggggtact cctacggcgg aacggacacc ctgagcaaga
       61 tcctcaagca gaaggtcttt aaggccgcgg agctgaaaaa cctgttgctc tgcctggacg
      121 ccgtcattat gctgatcatg ctctcagcct tcagcctgga cacggtggcc tacgcctttg
      181 taggacagat catctacgtg aacagcatga actacgtcat cttcaacatg ggtccccgcc
      241 tctatgagat ccagatcgtc tgtgaggacc cccgggccat cgagcgtttt atcatcgagg
      301 agatcggcaa gagcgccacc atctccaagg tcatcggggg ctactcccag gcggacaaga
      361 tccagctgga ctgtgtctgc aactcacggg agtatgtcca attgcgggag ttcatccggg
      421 acagcgggat cgactgcttc atcaaggtct tccccctcac ccacgtcttc ggccgcagca
      481 aggatttcca ccgcctcagc gaccagaatc tgtgacaaaa agcgggacac atatgtgtcc
      541 cgctttttct ccccattgcc caagaggcgg ttttcatttt ccctgttctg tggtacaatg
      601 ctcctgcgcc cctctggggc aggacttttg actttccgga gagagaccat ggaacaggaa
      661 ctttttttgc ccaccctgat gcactggcag tacggcaaca actggtgcgg cagcctgaag
      721 cgggccagct tctggatcac ccctaaagat gaacagctca ccgcccagct gtggaccgga
      781 ccggccatcc gggaaaacgc ggagatcctg tacaccgcac agttccccat cagcgacgag
      841 gggctggagc agctccgcac ctggctgctg gagcacgccg ctgctgtcaa cgccgccaac
      901 ccggagtcct tccgctacgc gtggtagccc ccggcgccgc tcttttccat ttcgatgaaa
      961 caggaggccg cggccccatg aatctgaatc aactctacta tctccaggcc atcgcccagg
     1021 ccaggggcct cacccgggcc gctgacgccc tctatgtcac tcagtccaac ctgagccact
     1081 ccatggccgc actggaggag gagctgggca tcccccttct ctacaaaaac gggcgggata
     1141 ccctgctcac tccgtacggg caggagttcc tcgcctacgc cgagcgggcc atccaggaga
     1201 tcgaggaggg gaagcgggtg gcccagtccc ggtgcagccc cacccggggc caggtacggc
     1261 tgggcttcat cagcgctgtc agcgccactc tgatcccctg gtgcgtcagt cacttctacc
     1321 agaacccgga caaccagggt attactttta cctttgacga gaaacccacc cggcgcatcg
     1381 ccgccgattt cacccaccac gccctggaca tcggcttcgg cacccggttc gacgacagct
     1441 catttgagtt ctgccccgtg gtgcccgagg agctggtggc cgtggtcccc gccggacatc
     1501 ccctggccca gcaggagcag gtgacgctgg agcagttgag ccgggagcgg ctggtgatct
     1561 acaaccaggc cagccccacc cgcaatctgg tgctgtccat gtttcgtcag caggatctcc
     1621 agccagatgt cgctttcgag gtggccaccg accacatgtt agccagcttt gtctcccagg
     1681 gactaggggt gggcatcatg ccccgcatgt tcggcctccg gctctactct gtccgtcccc
     1741 tctacatcgc ggacactgag acccaccgca tgctctatat gttccgcccc aaggaccgca
     1801 gggtcctgcc cgcggtccag cggttctggg actttgtgct ggaggtctgc gcccagtacc
     1861 agtccgacga atagatacgg aaaggcccgt cctccccgca tgcgcggggg aggacgggct
     1921 ctcttctgtc ccgccggctc acttgtccgg gtgcaggccg gaccagcctg tctggggcat
     1981 ggtccgcagc cgctccaagt cctccgcctc cagggcaaag tccagcgcca ggttctggcg
     2041 catccgtcca gcgtcggccg acttgggcag gggaaccacg ccgcagtcca gggcgaagcg
     2101 caggcagatc tgggcctggg agaccccgta tttcccggcc agctccgcca gcaggggatc
     2161 atccgccagc ctctggcggc ccagggggct ccatgcctcc accaggatct ggctgg
//
LOCUS       DBMDSJ010000298         2202 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11431_length_2202_cov_1.631128, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000298 DBMDSJ010000000
VERSION     DBMDSJ010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11431_length_2202_cov_1.631128"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="ACZPAQ_07320"
     CDS             <1..823
                     /locus_tag="ACZPAQ_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR0903835.1"
                     /translation="DLQGRIDILKVHAKQIRLAEDVDFEPIAKTAAGASGAELANIVN
                     EAALRAVRAGRPYATQEDLQESVEVVIAGYQKKSRVLSDQEKKIVAYHETGHALVAAL
                     QTHSAPVQKITIIPRTSGALGYTLQVDDGDHFLMSKDELLNKIATYTGGRAAEELVFH
                     SITTGASNDIEQATKLARSMISRFGMSDEFGFVAFETVSNQYLGGDAALACSPETQAR
                     IDRKVTEMVDTQYQKAMDLLKTNEEKLHQLAQYLYEHETITGREFMEILEGQREV"
     regulatory      914..1020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1326..1844
                     /gene="thiW"
                     /locus_tag="ACZPAQ_07325"
     CDS             1326..1844
                     /gene="thiW"
                     /locus_tag="ACZPAQ_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HIR0903836.1"
                     /translation="MNQSNTKKLAIAGILCAVAVVGSLFTFPVLASKCAPIQHMVNVL
                     CAVLLGPWYGVGVAFGASLIRNLLGLGTIMAFPGSMCGALLCGLVFWKTRNLPATLVG
                     EVFGTGIIGGLAAYPVAILFTSADAATVAFYAYIVPFLISTVAGAILAGILVAALGRT
                     GALSSMRTALER"
     gene            1950..>2202
                     /locus_tag="ACZPAQ_07330"
     CDS             1950..>2202
                     /locus_tag="ACZPAQ_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR0903837.1"
                     /translation="MDITPEQVRAHRLRAHHLDRRYPASRILEAAGACGLQNSPPGGW
                     ETALFQRLEGCSLPLLRDALYRDRALLQAWSIRGVPLVFP"
BASE COUNT          382 a          740 c          693 g          387 t
ORIGIN      
        1 cgaccttcag ggccgcatcg atatcctgaa ggtccacgcc aagcagatcc gtctggcgga
       61 ggacgtggac ttcgagccca ttgccaagac cgctgccggg gcctccggcg cagagctggc
      121 caacatcgtc aacgaggccg ctctgagggc ggtgcgggca ggccgcccct acgccactca
      181 ggaggactta caggagagcg tggaggtggt catcgcagga taccagaaga agagccgggt
      241 gctctccgac caggagaaga agatcgtggc ctaccacgag acgggccacg ccctggtagc
      301 cgccctccag acccactccg ccccggtgca gaagatcacc atcatccccc gcacctccgg
      361 tgcgctgggc tatactctcc aggtggacga cggtgaccat ttcctcatgt ctaaggatga
      421 gctgctgaac aagatcgcca cctacaccgg cggccgggcc gccgaagagc tggtcttcca
      481 ctccatcacc accggggcct ccaacgacat cgagcaggcc accaagctgg cccggtccat
      541 gatctcccgc ttcggtatga gcgacgagtt cggcttcgtg gcctttgaga cggtgagcaa
      601 ccagtacctg ggcggggatg ctgccctggc ctgctccccg gagacccagg cccgcatcga
      661 ccggaaggtg acggagatgg tggacaccca gtaccagaag gccatggacc tgctgaaaac
      721 caacgaggaa aagctccacc agctggccca gtacctctat gagcacgaga ccatcaccgg
      781 gcgggagttc atggagatcc tagagggaca gcgggaggtc tgacagacgg ccttctcctt
      841 ccctccaggg tccgccgcgc cagggtccga aaatttttct tgacaaaccg ggcctccggg
      901 cccataatac agttgcaaaa gacaggggag cctgcggcag cgggctgaga ggaagctgag
      961 cgcttcgacc cttcacctga tgtgggtcat gccaccgtag ggagtcatgc tggccgtgac
     1021 cagacggggt gcccaccggg caccccgttt tttgatcctt tccgaaggga gcgtgaggag
     1081 acacatcggc gccccggcct ggccgggagc gggcagaggg ggagcgccct gtgcccgggc
     1141 cgcatacagc agcgatggga agccgtgttc cggcgtgaga gggggccagt aagccccgac
     1201 cgaacccagg gaggaccctg tctgtccttc cgggacccga tttgtggccc ctcggggccg
     1261 gaaaaagggg agacgctgtc tgcttttgtg tgtttcccca tccattttga aaggagcact
     1321 ttcccatgaa tcagagcaac accaaaaagc tggccattgc cggcattctg tgcgccgtgg
     1381 cggtggtggg cagcctgttc actttccccg tcctggccag caagtgcgcc cccatccagc
     1441 atatggtcaa cgtcctgtgc gctgtcctgc tgggcccctg gtacggagtc ggggtggctt
     1501 tcggcgccag cctgatccga aacctgctgg ggcttggcac gatcatggcc ttcccaggca
     1561 gcatgtgcgg cgccctgctg tgcggactgg tgttctggaa gacacggaac ctccccgcca
     1621 ccctggtggg ggaggtcttc ggcaccggca tcatcggcgg cctggccgcc tatcccgtgg
     1681 ccattctgtt taccagcgca gacgccgcca cggtggcttt ctacgcctac atcgtcccct
     1741 tcctcatctc cactgtggcc ggtgctattc tggccggcat cctggttgcc gccctggggc
     1801 ggaccggggc cctgtcctcc atgcggaccg ccctggagcg ctgaatcccc acttcttttc
     1861 ccggcccgca gagcgggccg ggttttttgc ttttttgcct cctccggggt ataatgggac
     1921 caacgacgag acaagggagg tcttttccca tggacatcac cccggaacag gtccgcgccc
     1981 accggctgcg ggcccaccat ctggaccgcc ggtatcccgc cagccggata ctggaggctg
     2041 cgggggcctg cgggctccag aactccccgc ccggcgggtg ggagactgcc ctcttccagc
     2101 gcctggaggg ctgctccctc cccctcctcc gagacgccct gtaccgggac cgggccctgc
     2161 tccaggcctg gagcatccgg ggcgtgcccc tggtctttcc cg
//
LOCUS       DBMDSJ010000299         2197 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11459_length_2197_cov_1.687500, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000299 DBMDSJ010000000
VERSION     DBMDSJ010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11459_length_2197_cov_1.687500"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="ACZPAQ_07335"
     CDS             <1..798
                     /locus_tag="ACZPAQ_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR0903838.1"
                     /translation="LTQEERGCICPARHSFDRAAAGYVHLLPPNRMHSKAPGDDREMV
                     AARAAFLERGYYAPLRQALEELVLEASAHLEGPVLLDSGCGEGYYTAGMFRALERAGH
                     RPRAAGIDISKYALRRAAKRLPEGEFAVASAYRLPLADASADLLTNVFSPLSAEEFAR
                     VLRPGGTFLYVVPSARHLWEMKQVLYSQPYENPVKETPYTGFSYQRIVPVRYEITLDC
                     PEDIRALFQMTPYCWKTPREGVEALSALKRLTTQVGFDLHVFRRSPA"
     gene            complement(859..1911)
                     /locus_tag="ACZPAQ_07340"
     CDS             complement(859..1911)
                     /locus_tag="ACZPAQ_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding metallopeptidase"
                     /protein_id="HIR0903839.1"
                     /translation="MRRGKDRLIGAALCLLLLFGAGCSPAPGADQETLDRELDVQLGL
                     LAEVITQEERSAAMDLWMRLRPVRQARLDRGEYRRGDREQELLEELNGLYEAYTIRYL
                     GGDDLGWGYTDPEEHVLARYRIGGAGELTLMPNSLDLTHGPWTREDLEEMWESMQAVL
                     PKDAFRDFRSYVPFTDGEGETVAYVLPADPGGSQWEICLDPADMGDRDYFLETVLHEY
                     CHYLTLNHRQADYRGEPTVETYCEAGMVSREGSYLDDFCQRFWTGYPDDRLADLDSYN
                     FFLRHEEDFVSSYASTDPSEDISESFAFFVLWDVPESEAVWAEKLRFFLDYPELTEFR
                     RQVRENLGLEQPEQAA"
     gene            complement(1908..>2197)
                     /locus_tag="ACZPAQ_07345"
     CDS             complement(1908..>2197)
                     /locus_tag="ACZPAQ_07345"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR0903840.1"
                     /translation="QGAALWPEGIYAVGNAPTALIRMAQLIRAGAMSPALILAVPVGF
                     VNVVESKEEILALARERGIPAIAALGRKGGSTVAAALCNALLYQSGGREKL"
BASE COUNT          335 a          822 c          675 g          365 t
ORIGIN      
        1 ctgacgcagg aggagagggg ctgcatctgt cccgcccgcc acagctttga tcgggcggcg
       61 gcgggttacg tccacctcct gccccccaac cggatgcact ccaaggcgcc gggggacgac
      121 cgggagatgg tggccgcccg ggccgctttt ctggagcgcg gctactacgc cccgctgcgc
      181 caggccctgg aggagctggt cctggaggct tccgcccacc tggagggccc cgtcctgctg
      241 gacagcggct gtggggaggg ctactacaca gcggggatgt tccgggcgct ggagcgggcc
      301 ggacaccggc cccgggcagc ggggatcgat atctccaaat acgccctgcg ccgggccgcc
      361 aagcggcttc cggaggggga attcgcagtg gcctccgcct accgcctgcc cctggcggac
      421 gcctcggcgg acctgctgac caatgtgttc tcccccctgt ccgcggagga gttcgccagg
      481 gtcctgcggc cgggcggcac cttcctctat gtggtcccct ccgcccggca cctctgggag
      541 atgaagcagg tcctctattc ccagccctac gaaaaccccg tcaaggaaac cccctataca
      601 ggcttttcct atcagcgcat cgtcccggtg cggtatgaga tcactctgga ctgcccggag
      661 gacatccggg ccctgttcca gatgaccccc tactgctgga agactccccg ggagggggtg
      721 gaggccctgt ccgctctgaa gcggctcacc acccaggtgg gctttgacct ccacgtgttc
      781 cgccgcagcc ccgcctgatc tctcccgccg ccccataaaa tatgcccgga ggcacgtgtg
      841 cctccgggca tcctgcattt atgccgcctg ctccggctgc tccagcccca ggttctcccg
      901 cacctggcgg cggaactccg tcagctccgg gtagtccagg aaaaaccgca gcttctccgc
      961 ccacaccgcc tccgactccg gcacgtccca cagcacgaag aaggcgaagc tctcgctgat
     1021 gtcctccgac gggtcggtgg aggcgtagct gctgacgaaa tcctcctcat gccgcaggaa
     1081 gaagttatag ctgtccagat ccgccagccg gtcatccggg tagccggtcc agaaccgctg
     1141 gcagaagtcg tccagatagg acccctcccg gctgaccatt ccggcctcgc agtaggtctc
     1201 cactgtgggc tccccccgat agtccgcctg acggtggttc agggtgaggt agtggcagta
     1261 ctcgtggagc acggtctcca gaaaatagtc ccggtccccc atgtccgccg ggtccaggca
     1321 gatctcccac tggctccccc cggggtcggc gggcagcaca taggccaccg tctccccctc
     1381 gccgtccgta aagggcacat agctccgaaa gtcccgaaag gcgtccttgg ggaggacggc
     1441 ctgcatcgac tcccacatct cctccaggtc ctccctggtc cagggcccgt gggtcaggtc
     1501 cagggagttg ggcatgaggg tgagctcccc cgccccgccg atccggtagc gggccaggac
     1561 gtgttcctcc gggtccgtgt agccccagcc caggtcgtct ccccccagat accggatggt
     1621 gtatgcctca tagagcccgt tcagttcctc cagcagctcc tgctcccggt ccccccgccg
     1681 atactcgccc cggtccagcc gggcctggcg caccggtcgg agccgcatcc acaggtccat
     1741 ggccgcgctc cgctcctcct gggtgatgac ctccgccagc aggcccagct gcacgtccag
     1801 ctcccggtcc aacgtctcct ggtccgctcc cggggccggg gaacaccccg ccccaaagag
     1861 caggagcagg cagagggcgg ccccgatgag ccggtccttt cctcgtctca taatttctcc
     1921 cgtcctcccg actgatacag cagggcgttg cacagggcgg cggccaccgt gctccccccc
     1981 ttccggccca gagccgcgat ggcgggtatc ccccgctccc gggccagggc caggatctcc
     2041 tccttgctct ccaccacatt gacgaatccc accggcaccg ccaggatcag ggccggggac
     2101 atcgctccgg cacggatcag ctgggccatg cggatcagcg cggtgggggc gttgcccacg
     2161 gcatagatcc cctcgggcca gagggccgcc ccctggt
//
LOCUS       DBMDSJ010000300         2190 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11501_length_2190_cov_1.881762, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000300 DBMDSJ010000000
VERSION     DBMDSJ010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11501_length_2190_cov_1.881762"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="dnaJ"
                     /locus_tag="ACZPAQ_07350"
     CDS             <1..1020
                     /gene="dnaJ"
                     /locus_tag="ACZPAQ_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR0903841.1"
                     /translation="RFKEVNEAYEILSNKEKRARYDQFGHAGVDPNYGAGGGGFTGDF
                     GDIDLGDLFGSFFGGFGGGGFGSSAGRRNGPQKGESLRASLTITFEEAAFGCEKEINL
                     NRTEECDACHGSGCESGTTAEICPDCRGTGTVRIQRGAGGFAFTSTAPCSKCRGTGKI
                     IHSPCKACGGAGSVRKQKRIAVTIPAGIDDGQAVSLRGQGNAGKNGGPAGDLIVGVHI
                     QPHPQFQRDGTTVLYEQPISFYQAAMGAELEIPTIDGKVKYNLPAGTQTGTTFRLRGK
                     GIPELRGRGRGDQYVTVRVAVPTGLNGEQKEALRAFAAAMGEIPAQEEEEGLKGFFDK
                     RKRKK"
     gene            1322..1990
                     /locus_tag="ACZPAQ_07355"
     CDS             1322..1990
                     /locus_tag="ACZPAQ_07355"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR0903842.1"
                     /translation="MTNDASLCYVVGAMPLDPGFILTPVPGDLVIEADKGYETLSRLG
                     VHPGLVVGDFDSLGSVPDHPQVLRLPRVKDETDTGFALRQGLKRGYRRFVLLGCLGGR
                     LDHTVANLQLLSWLSAQGAQGVLLGEGEAAAVVTEGTLSFPASMEGFVSVFCAGGTAR
                     GVTLEGLKYPLDHAELTSDFPLGVSNEFVGLPARISVEEGSLLVAWEDRGTLDDLLPT
                     LWSD"
BASE COUNT          388 a          686 c          717 g          399 t
ORIGIN      
        1 aggttcaaag aggtcaacga ggcctacgag atcctgtcca acaaggagaa gcgggcccgg
       61 tatgaccagt ttggccacgc cggggtggac cccaactacg gcgctggcgg gggcggcttc
      121 actggagact ttggagacat tgacctgggc gacctgttcg gctccttctt cggcggcttc
      181 ggcggagggg gcttcgggtc ctccgctggc cggcgcaatg gcccccagaa gggagagagc
      241 ctgcgggcca gcctcaccat cacctttgag gaggccgcct tcggctgcga gaaggagatc
      301 aacctgaacc gcaccgagga gtgtgacgcc tgccacggct ccggctgtga gtcagggacc
      361 acagcggaga tctgccctga ctgccggggc accgggaccg tccgcatcca gcggggggcg
      421 ggcggctttg ccttcaccag tacggcgccc tgctccaagt gccgggggac cggcaagatc
      481 atccacagcc cctgcaaggc ctgcgggggg gcgggcagcg tgaggaagca gaagcgcatc
      541 gctgtgacca tccccgccgg catcgatgac ggccaggctg tctctctgcg cggacagggg
      601 aatgcaggca agaacggcgg tcctgccggc gatttgatcg taggcgtcca tatccagccc
      661 catccccagt tccagcggga cgggaccacg gtgctgtatg agcagcccat ctccttctac
      721 caggcggcca tgggggctga gctggagatc cccaccattg acggaaaggt aaagtacaac
      781 ctgcccgccg gcactcagac cggcaccacc ttccggctga gaggaaaggg catcccggag
      841 cttcggggcc gcgggcgggg cgatcagtac gtcaccgtcc gggtggcggt gcctactggg
      901 ttgaatggag agcagaagga ggccctgagg gctttcgccg ccgctatggg ggagatccct
      961 gcacaggagg aggaagaggg gctcaagggc ttctttgaca agcggaaacg gaaaaaataa
     1021 gttcggatct caatcggcga gtccggggcg gccgtacagg gccgccccgg acccctgttg
     1081 ccgggagctt gcggggcgca cgatgtgcgc cccgtatttt ttgacccagc cttgttcttc
     1141 ctctgcgggg cgcatgtcct tctgtttcct tttctccgcc ccttctgtat atgtttttct
     1201 ccacaagggg aaattttatg ttataataga aaaaacgtga tctcattcag cttgccagct
     1261 ccatccctgc ctgggggccg gcacccatct cagctctgct gtccacggaa gggagaccca
     1321 tatgacaaac gatgcatcgc tctgctatgt ggtgggggcc atgcccctgg accccggttt
     1381 catcctcacg cctgtccctg gcgacttggt cattgaggcc gacaagggct atgagaccct
     1441 gtcccggctg ggcgtccacc ccggccttgt agtaggggat ttcgactcgc tgggctccgt
     1501 cccagaccat ccccaggtcc tccgcctccc cagggtcaag gacgagacgg acaccggctt
     1561 cgccctgcgc caggggctga aacggggata ccggcgcttt gtgctgctgg gctgtctggg
     1621 cgggcgtctg gaccacacgg tggccaatct tcagctgctc tcctggctct ccgcccaagg
     1681 ggcccagggc gttctgctgg gagaaggcga ggcggcagcc gtggtcactg aagggacgct
     1741 ctccttcccc gcctccatgg agggcttcgt ctccgtgttc tgcgccggcg gtactgcccg
     1801 gggcgtcacc ctggagggtc tgaaataccc cctggaccac gcggagctca ccagcgactt
     1861 ccccctgggg gtcagcaatg agttcgtggg cctgcccgcc cggatctccg tggaggaggg
     1921 ctcccttttg gtggcctggg aggaccgggg gaccctggat gacctgcttc ccaccctgtg
     1981 gagcgactga aaaggagcaa tttgcaatga aatactattt tgttataagg gaggagctcc
     2041 cccatgcctg accagagcag cgagaaggac ctgaggctgg ccgtgctcat cgatgcggac
     2101 aacgcctccc gcaccgccat gaaggatgtg atggctgagg tggccgtcta cggcaccccc
     2161 accatcaagc gcatctacgg cgactggacc
//
LOCUS       DBMDSJ010000301         2187 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11520_length_2187_cov_1.887824, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000301 DBMDSJ010000000
VERSION     DBMDSJ010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11520_length_2187_cov_1.887824"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1855)
                     /locus_tag="ACZPAQ_07360"
     CDS             complement(<1..1855)
                     /locus_tag="ACZPAQ_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 aminopeptidase family protein"
                     /protein_id="HIR0903843.1"
                     /translation="MKALHLFCLELGRLFQSRLTWLVILLTVLSPLAGLVLYKPTAAD
                     TMLSMYLADPALAGGVVGSILFGLLTVYELDRTERSRVGMLMDAAVSPLTMALVRLPA
                     LAAAAVLGLGLTMLVWLPISRELIGSVFGGTDYLLSYLLFMGLALWLAILAAAAAYQF
                     TRRSDLSLVLFAAFAALSLTVWAGKWQLCWLNPCVWALSDDFSNFRLYRSVAYMRLTW
                     LAALAGVWTLSWLCIRQYGKGLLGSLARSARRVCRPLIALLLLACSGAAYAAQPFFDN
                     SNPDLTASRLFELEYAEGVTCSRRTAQVFPDTAAGTVEGRATYQFQNTSGQGWTVCFG
                     VDPGYDITSARANGAEISPVRTGYEESNMALWEIALPADGAVELVLEYGGFPRDWNIA
                     ETTQGSPEISGAYLCLENQNLMPYLLNVLPEGQGYPTTVELTVPGSMTVIPFGISEAE
                     VVGTHGNGTATWRYEDDSAGGIVYAGDYIRQDIQAGGATIQLYYGRKHQAVMEQAGAA
                     DAVRAVVDYCTEHYGPLSFSAGGSLKLIQSRVAGGGYAAGGASLLDEMDFTAANLRDG
                     GKGAVPGEVMIHELVHQWWGLGNMFDSEDPADPWSAEGLTVYTTYRIVKELY"
     gene            complement(1852..>2187)
                     /locus_tag="ACZPAQ_07365"
     CDS             complement(1852..>2187)
                     /locus_tag="ACZPAQ_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0903844.1"
                     /translation="SQTAQDRLVLLSTHIIEDVQSVCGQLVVIDQGRVLFTGTPEALI
                     QSAAGHVGVFWEREAAQEAGLHITARVNTSQGIRCRAVADVLPRFAQPAEPTLEDAYL
                     YLISREAVQ"
BASE COUNT          417 a          715 c          735 g          320 t
ORIGIN      
        1 cgtagagctc cttgacgatg cggtaggtgg tgtacaccgt cagcccctcg gcggaccagg
       61 gatctgcggg gtcctcggag tcaaacatgt tgcccaggcc ccaccactgg tggaccagct
      121 cgtggatcat cacctcgccg gggacggccc ccttgccgcc gtcgcggaga ttggcggcag
      181 tgaagtccat ctcgtccagc aggctggcgc ccccggcggc gtagcccccg ccggccaccc
      241 ggctctggat cagcttcaga gagccgccag cgctgaagga cagggggccg tagtgctctg
      301 tgcagtagtc caccacggcc cgcaccgcat ccgccgcccc ggcctgctcc atcaccgcct
      361 gatgcttccg gccatagtag agctggatgg tggcgccgcc ggcctggata tcctgtcgga
      421 tgtagtcccc ggcgtagacg atgccgccag cgctgtcatc ctcatagcgc caggtggcgg
      481 tgccgttgcc gtgggtcccc accacctctg cctcgctgat accgaagggg atgacggtca
      541 tggagccggg cacggtgagc tccacggtgg tgggatagcc ctgcccctct ggcagaacat
      601 tgagcagata gggcatcagg ttctgattct ccaggcacag ataggctccg ctgatctcgg
      661 gggagccctg ggtggtctcc gcaatgttcc agtccctggg gaagccgccg tactccagca
      721 ccagctccac cgcaccgtcc gccgggaggg cgatctccca cagagccatg ttggactcct
      781 cgtagccggt gcggacgggg gagatctccg ccccattggc acgggcagaa gtaatgtcgt
      841 accccgggtc cacaccgaag cagaccgtcc acccctggcc ggaggtgttc tggaactgat
      901 aggtggctcg gccctccacc gtgccagcgg cggtgtctgg gaatacctgg gcggtgcgcc
      961 tggaacaggt gactccctcg gcatactcca gttcgaagag ccgggaggcg gtgaggtccg
     1021 gattgctgtt gtcaaagaag ggctgagctg cataggccgc accggaacag gccaggagca
     1081 gcagggcgat caacggccga cagacccgcc gggcgctccg ggccagggaa cccagaagcc
     1141 ccttcccata ctgccggata cacagccagg agagggtcca aacccccgcc agggctgcca
     1201 gccaggtcag gcgcatatag gccaccgacc ggtagagccg gaagttggag aagtcatcgg
     1261 acagggccca tacgcaggga ttgagccagc acagctgcca cttcccggcc cacaccgtca
     1321 gactcagggc ggcgaaggcg gcaaagagga ccagggacag gtcagagcgc cgggtgaact
     1381 gataggcggc ggccgctgcc agaatggcca gccacagggc cagccccatg aacagcagat
     1441 aggagagcag gtagtccgtg ccgccaaaga cggagccgat gagctcccgg ctgatgggga
     1501 gccaaaccag catggtgagg cccagaccca ggacagcggc agcggccagg gcgggcaggc
     1561 gcaccagggc catggtcagg ggggagaccg cagcgtccat cagcatgccc acccggctgc
     1621 gctccgtgcg gtccagctcg tacactgtca gcaggccgaa caggatgctc cccaccacgc
     1681 ccccggccag ggcgggatca gccagataca tggacagcat ggtgtcggcg gcggtgggct
     1741 tgtacagcac cagcccagcc agtggggaga gcacagtcag taaaatgacc agccaggtca
     1801 ggcggctttg gaatagacgt cccagctcca gacaaaacag atgcagagct ttcattggac
     1861 cgcctcccgg gagatgaggt agaggtaggc gtcctccagg gtgggttctg cgggctgagc
     1921 gaagcggggg aggacatcgg ccaccgcccg gcaacggatg ccctggctgg tgttcacccg
     1981 ggccgtaatg tgcagccccg cctcctgggc cgcctcccgt tcccagaaca cccccacgtg
     2041 tccggcggcg gactggatca gggcttccgg agtgccggtg aacaggaccc tgccctggtc
     2101 tataacgacc agctggccgc agaccgactg cacatcctca atgatatggg tggacagcag
     2161 caccagccgg tcttgggcgg tctggga
//
LOCUS       DBMDSJ010000302         2181 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11555_length_2181_cov_1.859135, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000302 DBMDSJ010000000
VERSION     DBMDSJ010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11555_length_2181_cov_1.859135"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            28..753
                     /locus_tag="ACZPAQ_07370"
     CDS             28..753
                     /locus_tag="ACZPAQ_07370"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR0903845.1"
                     /translation="MEQEQRTLQKWVDESRRIVFFGGAGVSTESGIPDFRSVDGLYHQ
                     KYDYPPEEILSRSFFDARPEEFYRFYREKMLCLDAQPNPAHRKLAELEAAGKLTSVVT
                     QNIDGLHQRAGSRRVWELHGSVHRNHCMRCGRAYPVEFVRDSGGVPRCACGGIVKPDV
                     VLYEEQLDGHTLQGALSDIQGADMLIIGGTSLAVYPAASLVNYYRGHRLVLINKSPTP
                     YDAYADLVIVGPIGEILGGVAVV"
     gene            763..2019
                     /locus_tag="ACZPAQ_07375"
     CDS             763..2019
                     /locus_tag="ACZPAQ_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024086.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR0903846.1"
                     /translation="MKNGRLFELLYLLVERRALTAGELAERFEVSERTIYRDVDALSA
                     AGVPVYTQRGQGGGIRLMDQFVLDRCLLSPREQDEVLFALRALLSTGGLEGAEALERL
                     SALFRREAEGWVEVDFTDWGSGEQERENFRIVREGILGRRLLDFTYYASSGTRTRRRV
                     EPGKLVFKSGCWYLQAFCLARRDWRIFRLARMEGLCLEREGFPPRRPPEQLEVPSPEQ
                     RQNGTLTLRFAPSAAWRVRDYFAPGQIRAGEDGCLLVECDFPQDGWLLGFLLSFGDQV
                     EVLAPASWREVLGRTGKKIAALYKTGHSVSGIQAYPEGTEEGSTPPEQKEEPDMEERI
                     FCQCCGMPLDAPEDMGSNADGSPNRDYCRWCYQRGAFTAPEATVEDMIAFDLKFNEEN
                     GHPFGPQEEAERLLRAWLPTLKRWRK"
BASE COUNT          374 a          639 c          789 g          379 t
ORIGIN      
        1 gacagcggcc aggaaaggag catggatatg gaacaggagc agaggaccct gcagaaatgg
       61 gtcgatgaga gcagacgcat cgtctttttc ggcggggcgg gcgtgagcac ggagtcgggc
      121 atccccgact tccgcagcgt ggacgggctg tatcaccaga agtatgacta tcccccggag
      181 gagatcctca gccgcagctt ttttgatgcc cggccggagg aattttaccg cttttaccgg
      241 gagaagatgc tctgcctgga cgcccagccc aacccggccc accggaagct ggcggagctg
      301 gaggcggcgg ggaagctgac cagtgtggtg acccagaaca tcgacggact gcaccagcgg
      361 gcgggctccc ggcgggtgtg ggagctccac ggaagcgtcc accgcaacca ctgcatgagg
      421 tgcggcaggg cctatcccgt ggagttcgtg cgggacagcg gaggtgtgcc ccgatgtgcc
      481 tgcgggggca tcgtcaagcc cgatgtggtg ctctatgagg agcagctgga cgggcatacc
      541 ctgcaggggg ctctttcgga catccagggg gcggatatgc tcatcatcgg cggcacttca
      601 ctggcggtgt accccgccgc cagcctggtg aattactacc gggggcaccg cctggtgctc
      661 atcaacaagt cccccacccc ctacgacgcc tacgccgacc tggtgatcgt cggccccatc
      721 ggggagatcc tgggcggcgt agcggtggtg tgagggcgcg ggatgaaaaa cggccgcctg
      781 tttgagctgc tctatctgct ggtggagcgc cgcgccctca ccgccgggga gctggcggag
      841 cgcttcgagg tgtcggagcg gaccatctac cgggacgtgg acgccctttc cgccgccggt
      901 gtgccggtct atacccagcg gggacagggg ggcggcatcc gcctgatgga tcagttcgtg
      961 ctggaccgct gcctcctctc ccccagggag caggatgagg tgctctttgc cctgcgggcc
     1021 ctgctgtcca ccggcgggct ggagggggcg gaggccctgg aacggctgtc cgccctgttc
     1081 cggcgggagg cggagggctg ggtggaggtg gacttcaccg actggggcag cggggagcag
     1141 gagagggaga atttccgcat cgtgagggaa ggcatcctgg ggcgccgcct gctggatttc
     1201 acctactacg cctcctccgg cacccgcacc cgccgcaggg tggagccggg caagctggtg
     1261 ttcaaatccg gctgctggta tctccaggca ttctgtcttg ccaggaggga ctggaggatt
     1321 tttcgacttg cgcggatgga ggggctgtgt ctggaacggg agggctttcc tcccagacgg
     1381 ccgccggagc agctggaggt tccttccccg gaacagcggc agaatgggac cctgaccctc
     1441 cgttttgccc cctccgcggc ctggcgggtg cgggactatt ttgctccggg ccagatccgg
     1501 gcgggagagg acggctgcct gctggtggag tgtgactttc ctcaggacgg gtggctgctg
     1561 ggctttcttc tctctttcgg agaccaggtg gaggtgctcg cccctgcctc ttggcgggag
     1621 gtcctgggcc ggactgggaa aaagatcgcg gccctgtata aaactggaca ttccgtgtca
     1681 ggtatccagg cgtatcctga gggcacagag gaggggagca cccctcccga acaaaaggag
     1741 gaacctgata tggaagagcg gatcttttgc cagtgctgcg gcatgcccct ggatgccccc
     1801 gaggacatgg ggagcaacgc cgacggaagc cccaaccggg attactgccg ctggtgctat
     1861 cagaggggcg cattcaccgc gccggaggcc acagtggagg acatgatcgc ctttgacctg
     1921 aaattcaatg aggagaacgg acatcccttc ggcccccagg aggaggcgga gaggctcctg
     1981 cgggcctggc tccccacctt gaagcggtgg cggaagtagg aaatggcaat tggggcaggg
     2041 cctgcggctg gccggctttt ctccgtgcag ggtgtcgggc gcatgatatg cgcccctgcg
     2101 aaatagacgg agccctacgc atcccccaag ggggaaggcg tgggggcgtc cttctgcggg
     2161 gccggatcaa caggatggac a
//
LOCUS       DBMDSJ010000303         2160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11685_length_2160_cov_1.126275, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000303 DBMDSJ010000000
VERSION     DBMDSJ010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11685_length_2160_cov_1.126275"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2160
                     /locus_tag="ACZPAQ_07380"
     CDS             <1..>2160
                     /locus_tag="ACZPAQ_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0903847.1"
                     /translation="LERAADSIEKAGRLLKSGIGKIIDGLEQLRNFVRIENETGSREA
                     LEKIEEGVGETAQAAEELGRAMDLAAQSGVVEDVAAQSMEELGGEMGQISDALTQVGG
                     GAETVRLNISFDLEDIREGLRQIRSGVKNVVDSSGAAMKAVDSLGDALDELKAVGQEL
                     SDGLDALADAVSSFEQAGETGTEVFAGLEDLFGYLAGVDPIQIAHPGEEVSAASDALY
                     DTMERLNDQMGLLNDTAAASVGDLTGDIRAISAQVGVVTDTLIDAIREIENYDLEDSI
                     SDTSEENIDQVTSGRLLSCSNTAAISGDLNVGGVAGSMAVEYELDPEDDLISNSAPIY
                     RRAYELKAILQRCTNTGAVVGRRDHVGSVCGRMDLGLILECEAYGSAESENGDYVGGV
                     AGYAVGTIRESFAKCALSGKRYVGGIVGAAGTRDSGGSVTGCCSFVLIPEYEEYAGAI
                     SGTDQGLFQNNRFVSDDLAGLNRVSAAGRAEPISYEQLLQMEDIPTPMKRLELRFWAD
                     GRVIQTVPFDYGASFDDSILPEIPHQEGCFAVWDREDLTDLRFDTDVTAVYTAYRSAL
                     SSAQLRDDGRPVLFVEGEFDQGDSLEARLLETREDYPRQLQTLADRRRVLERWKVTIP
                     ADGQSIHTVRYLSPNGKTERTEFYAVSEDRWTRLDTETAGSYLLVRLEGDTPEIAVVT
                     RTPIWWVWPIAAALVAGLVFCAVGWAKHRRLLQRAVA"
BASE COUNT          431 a          555 c          768 g          406 t
ORIGIN      
        1 actggagcgg gcggcggaca gcatcgagaa ggccggccgg ctgctgaaaa gcggcatagg
       61 aaagatcatc gacgggttgg aacagctgcg gaacttcgtc cgcattgaga atgagactgg
      121 cagccgggag gccctggaaa agattgagga gggggttgga gagaccgccc aggcggcaga
      181 ggagctggga agagccatgg atctggcggc ccagtccggc gtggtggaag acgtggccgc
      241 ccagagcatg gaggaactgg ggggagagat ggggcagatt tctgatgccc tgacccaggt
      301 gggcggcggc gcggaaaccg tgcgtctgaa catctctttc gatttggagg atatccggga
      361 gggcctgcgg cagatccgga gtggtgtgaa aaacgtggtg gactcctccg gcgcggccat
      421 gaaggcggtg gacagcctgg gggatgccct ggatgagttg aaggccgtgg ggcaggaact
      481 gagtgacggc ctggacgccc tggcggatgc cgtgagctcg tttgaacagg cgggagaaac
      541 tgggacagaa gtgtttgctg ggctggagga tctgttcggc tacttggctg gggtggaccc
      601 tatccagatc gcccaccctg gggaggaggt cagcgccgcc tctgacgccc tctatgacac
      661 gatggaacgg ctgaacgacc agatgggcct cctgaatgac acggctgccg cctcggtagg
      721 cgacttgacc ggcgacatcc gggccattag tgcccaggtc ggggtggtga cagatactct
      781 gattgacgcc atccgggaga tcgaaaatta cgacctggaa gacagtatct cggatacctc
      841 agaggagaat atcgaccagg tgaccagcgg caggctcctg tcctgctcca acacggcggc
      901 catctccggg gatctcaatg taggcggagt ggctggctcc atggcagtgg agtatgagct
      961 ggaccctgag gatgacctga tttccaacag cgcgcctatc taccggagag cctatgagct
     1021 gaaggccatc ctccagcggt gtaccaacac cggagccgta gttggccggc gggaccatgt
     1081 aggcagtgtc tgcggccgga tggatttggg gcttatcctg gagtgtgagg cctacggcag
     1141 cgcggagagc gagaacggag actatgtggg cggtgtcgcc ggctatgcgg tggggacgat
     1201 acgggagagt tttgccaaat gcgccctgtc cggaaagcgt tatgtgggcg gtatcgtggg
     1261 ggccgccgga accagggact ccgggggatc agtcaccggg tgctgttcct tcgtccttat
     1321 cccagagtat gaggagtatg ccggtgcgat atccggcaca gatcagggcc tattccagaa
     1381 taaccgattt gtgtcagacg atttggctgg cctaaaccgc gtcagtgccg ccggccgagc
     1441 ggaacctatt tcctacgaac aactgctcca gatggaggat atccccaccc ctatgaagcg
     1501 gctggagctg cgcttctggg cggatggccg agtgatccag acggtcccct ttgactatgg
     1561 cgcttccttt gacgattcga ttttgccgga gatcccccac caggagggat gctttgcggt
     1621 gtgggaccgg gaggatctga cggatctgcg gtttgacaca gatgtgaccg cggtctacac
     1681 cgcataccgc tccgcgctgt cctctgccca actgcgggac gacggccggc cggtcctctt
     1741 tgtagagggg gagttcgacc agggggacag tctggaagcc aggctgctgg aaacgaggga
     1801 ggattatcct agacagctcc agaccctggc ggaccgtcgt agggtcttag agcggtggaa
     1861 ggtgaccata ccggcggatg gacagtctat tcatacggta cgttacctat ctcccaacgg
     1921 aaagacggag cggacggagt tctatgctgt ttccgaggat agatggaccc gtctggatac
     1981 ggaaacggct ggcagctatc tgctggtccg gctggagggg gatacccctg agattgcggt
     2041 agtgacccgg actccaatat ggtgggtctg gccgattgcg gcggccctgg tggccggact
     2101 cgtgttttgt gcggtgggct gggcgaaaca ccgcaggctg ctccaaaggg ctgtagcaca
//
LOCUS       DBMDSJ010000304         2156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11714_length_2156_cov_1.620438, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000304 DBMDSJ010000000
VERSION     DBMDSJ010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11714_length_2156_cov_1.620438"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="ACZPAQ_07385"
     CDS             <1..552
                     /locus_tag="ACZPAQ_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0903848.1"
                     /translation="SDLCAKRDWLLLIDEVQTGVGRTGSLFAFQQYGIQPDAVSFAKG
                     IAGGLPFGGVMAGEKCRNVFTTGTHGTTFGGNPISAAAACYVLDRMDDAMFQSVQEKG
                     AYLRSRIEALDLPCLGATRGMGLMIGIDVKGERTNKELAAKLIQSGLLVLTAGPGLRL
                     LPPLVITQEEMDKGLAIMKAALS"
     gene            618..1541
                     /gene="argF"
                     /locus_tag="ACZPAQ_07390"
     CDS             618..1541
                     /gene="argF"
                     /locus_tag="ACZPAQ_07390"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR0903849.1"
                     /translation="MQKDLLKLLDLSKEDIIKILNTADQLKYSQKHGLVHDYLRGKTL
                     AMIFEKNSTRTRVSFETGMYQLGGHALFLSGKESQIGRGEPIEDTARVLARYCDGIMI
                     RTFHQEEVESLASHSHIPVINGLTDFCHPCQVLADLMTVREHKATLEGLKLCYIGDGN
                     NMANSLIVGGLKCGMEVSVACPAGYDPDPQVLAFAASQPDFKFSLVRTPQEAAAGADV
                     VFTDVWASMGQEEERAVRAAAFAGYQVNDRLMTLTNPGCMVQHCLPAHRGEEITAEVF
                     EAHAQEIFDEAENRLHAQKAVMYLLMCPDAK"
     gene            complement(1828..>2156)
                     /locus_tag="ACZPAQ_07395"
     CDS             complement(1828..>2156)
                     /locus_tag="ACZPAQ_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HIR0903850.1"
                     /translation="DIMELCLFLLERKQTASQIGVGTLCSQLSDSPKTMEQRVRRAVE
                     RGLGHIASLGVEDYGNEFFTQYAAVLFPFPEVRAEMAHLRGKGGRGKVNLKKFLDGML
                     ILTEEY"
BASE COUNT          403 a          728 c          640 g          385 t
ORIGIN      
        1 tccgacctgt gcgccaagcg ggactggctg ctcctcatcg acgaggtgca gaccggcgtg
       61 ggccgcaccg gctctctctt tgcctttcag cagtacggca tccagcctga cgcagtctcc
      121 ttcgccaaag gcatcgccgg cggcctgccc ttcggcggcg tgatggcggg agagaagtgc
      181 cgcaatgtct tcaccaccgg cacccacggc accaccttcg ggggcaaccc catctccgcg
      241 gccgcagcct gctacgtgct ggaccggatg gacgacgcca tgttccagtc cgtccaggag
      301 aagggggcat atctccgcag ccgcattgag gccctggacc tgccatgcct gggcgccacc
      361 cggggcatgg gcctgatgat cggcatcgac gtgaaggggg agcgcaccaa caaggagctg
      421 gccgccaagc tgatccagag cggcctgctg gtcctcaccg ccggccccgg cctgcgcctg
      481 ctcccccccc tggtcatcac ccaggaggag atggacaagg gcctggccat catgaaagca
      541 gcgctttctt aacttcagcg cggagggcag gcccgctccc tctgtcatcc tcactctgaa
      601 catcgggggt ttttatcatg cagaaagact tactcaagct gctggatctg tccaaggagg
      661 acatcatcaa gatcctcaac accgctgacc agctgaaata cagccagaag cacggtctgg
      721 tccacgacta tctccggggc aagactctgg ccatgatttt tgagaagaac tccacccgga
      781 ccagggtctc ctttgagact ggaatgtacc agctgggcgg acacgctctg ttcctgtccg
      841 gcaaggagag ccagatcggc cggggcgagc ccatcgagga caccgcccgg gtcctggccc
      901 gctactgcga cggcatcatg atccgcacct tccatcaaga ggaggtggaa tccctggcca
      961 gtcactccca catccccgtc atcaacggcc tgaccgactt ctgtcacccc tgccaggtgc
     1021 tggccgacct gatgaccgtc cgggagcaca aggccaccct ggagggattg aagctgtgct
     1081 acatcgggga tggcaacaac atggccaact ccctcatcgt gggcggcctg aagtgcggca
     1141 tggaggtatc ggtggcctgc cccgccggct acgaccctga cccccaggtg ctcgcatttg
     1201 ccgccagcca gccggacttc aaattctccc tggtccgcac cccccaggag gcggccgccg
     1261 gggccgacgt ggtctttact gacgtgtggg cctccatggg ccaggaggag gagcgtgccg
     1321 tccgggccgc cgcctttgcc ggctatcagg tcaacgaccg ccttatgacc ctcaccaacc
     1381 cggggtgtat ggtacagcac tgcctgcccg cccaccgtgg ggaggagatc acggcggagg
     1441 tctttgaggc ccacgcccag gagatctttg acgaggcgga aaaccgcctc cacgcccaga
     1501 aggccgtcat gtacctgctg atgtgccccg acgccaaata aatcagacag agggccgccc
     1561 cgggacaacg ttccggggcg gcccgactgt cgaaaaagcg gcagagccgc tttttcgaga
     1621 aagcaagcac gcaagctggg tcccgatccc ttgcaggaaa gtgggcccat cttgtgtaag
     1681 aggtgtcatt tccgaggtac acgcccccgg aaatgacgtg atttaatttg attccgtcgc
     1741 ctgcgggcaa cggaactcct tgcaggaggg cttttttgac aagctggccg ccccgggacg
     1801 ttgtcccagg gcggccagct gtgcgcttca gtattcctct gtcaggatca gcatgccgtc
     1861 caggaatttt ttcaggttca ccttgccgcg tccccctttt ccccgcagat gggccatctc
     1921 agcccgcacc tcgggaaagg ggaagagcac cgcggcatac tgggtgaaga actcgttccc
     1981 atagtcctcc acccccaggc tggcgatatg gcccagcccc cgctccaccg cccgccggac
     2041 ccgctgctcc atggtcttgg ggctgtcact gagctggctg cacagggtgc ccactccgat
     2101 ctgagaggcc gtctgctttc gctccagcag gaagaggcac agctccatga tgtccc
//
LOCUS       DBMDSJ010000305         2145 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11787_length_2145_cov_1.796477, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000305 DBMDSJ010000000
VERSION     DBMDSJ010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11787_length_2145_cov_1.796477"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="ACZPAQ_07400"
     CDS             <1..202
                     /locus_tag="ACZPAQ_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903851.1"
                     /translation="VTGSVQDWTLTCSVHGEGEEGPVYTYTGGGADSSTVTVDTGDEV
                     SGVVSAEPKESGLTAASVTPAG"
     gene            complement(298..1653)
                     /locus_tag="ACZPAQ_07405"
     CDS             complement(298..1653)
                     /locus_tag="ACZPAQ_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-binding di-iron domain-containing
                     protein"
                     /protein_id="HIR0903852.1"
                     /translation="MHCVRNVTEDLIWIGGNDRQSPRFESDHPVPRGMSYNSYLLLDE
                     KTVLFDTVDRAVQHQFFENLEHALQGRRLDYVFVHHMEPDHAATLGDLLVRYPGTTIV
                     CNGKSMNLLRQFHWSAPKGAMLVKEGDTLCTGRHTFTFYSAPMVHWPEVMVSYDAADH
                     ILFSADAFGTFGALNGVLFADEVDFDRDWLDEARRYYTNIVGKYGPQVLALLKKVEGL
                     DVRMVCPLHGPIWRRDLGYLMDKYKKWASYQPEVRGVLIACASIYGGTETAAGILACR
                     LAERGIPVELYDVSVTHCSYVLSDAFKYSHIVFASATYNNGIFTPMEELLRDIAHHAL
                     QDRTVALIQNGSWAPASGKLMAQILGEMKNMELLEQTVTLKSALAPGQDQELEALADA
                     LAASVRGEQEAPEQAVADAPEAKGFICKICGFIYESDTLPEDFRCPICGRPASDFEPV
                     T"
     gene            complement(1847..>2145)
                     /locus_tag="ACZPAQ_07410"
     CDS             complement(1847..>2145)
                     /locus_tag="ACZPAQ_07410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF036850.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR0903853.1"
                     /translation="DILINCTSLGMTGTNSQFRDLSFLDELPGHAAVFDLIYSPAETE
                     LLRQARLRGLKAANGLGMLIYQAVFALEYFAGVRINAKEMEQHVIEACGLGGEA"
BASE COUNT          380 a          694 c          662 g          409 t
ORIGIN      
        1 cgtcactggc agtgtgcagg actggaccct cacctgctcg gtccacggcg agggcgagga
       61 gggaccggtc tacacctata ccggtggggg agcggattcc tccaccgtca cggtggacac
      121 gggagacgag gtctccggtg tggtctcggc ggagcccaag gagtctgggc tcaccgccgc
      181 ctcggtcacc ccggccggct gagctctgaa cgccagcggc gtttccccgg aggtatcccc
      241 agaggagcag cgggtccccc ggcgcggcga tgccgcccgg gggacccgct cattctctca
      301 ggtgaccggc tcaaaatcag aggccggacg gccgcagatg gggcaccgga agtcctccgg
      361 cagggtgtcc gactcgtaga tgaagccgca gatcttgcag ataaagccct tggcctcggg
      421 ggcgtccgcc accgcctgct ccggcgcttc ctgctccccc ctcacggagg cggccagggc
      481 atcggccagc gcctccagct cctggtcctg ccctggagcc agggcggact tcaacgtcac
      541 ggtctgctcc agcagctcca tgttcttcat ctcccccagt atctgggcca tcagctttcc
      601 gctggctgga gcccaggagc cgttctggat cagagccacc gtccgatcct ggagggcgtg
      661 atgggcgatg tcccgcagca gctcctccat gggggtgaaa atgccgttgt tataggtggc
      721 ggaggcaaag acgatatggc tgtacttaaa ggcgtcagac agcacgtagg agcagtgggt
      781 caccgacacg tcatacagct ccaccgggat gccccgctcc gccaggcggc aggccaggat
      841 accggccgcc gtctccgtcc ccccgtagat ggaagcgcag gcaatgagca ctccccgcac
      901 ttccggctgg taggaggccc actttttata tttgtccatc agatagccca ggtccctgcg
      961 ccagatcgga ccgtgcaggg ggcaaaccat ccggacgtcc agcccctcca cctttttcag
     1021 cagggccagc acctgagggc cgtacttgcc cacgatgttg gtgtagtagc ggcgggcctc
     1081 gtccagccag tcccggtcaa aatctacctc atcggcaaac agcacgccgt tgagggcgcc
     1141 gaaggtgccg aaagcatcgg cggagaaaag gatgtggtcc gcagcgtcat agctcaccat
     1201 tacctccggc cagtggacca tgggggcgga gtaaaaggtg aaggtgtgcc gccccgtgca
     1261 cagggtgtcc ccctccttca ccagcatggc ccccttgggg gcgctccagt ggaactggcg
     1321 gagcaggttc atcgacttcc cattacagac gatggtagtc ccggggtagc ggaccagcag
     1381 gtcgcccagg gtagcggcgt ggtcgggctc catgtggtgg acgaatacat agtccagcct
     1441 ccggccctgg agggcgtgct ccaggttctc gaagaactgg tgctggaccg cccggtccac
     1501 cgtgtcgaag agcaccgtct tctcatccag cagcaggtag gagttgtagg acatccccct
     1561 ggggaccggg tggtcacttt caaagcgggg gctctgccgg tcgttgccgc cgatccagat
     1621 cagatcctct gtcacgttcc gcacacaatg catatcataa accttctttc gtatggaaaa
     1681 tccttctgct cttttatcct accacatagg ttatcccggc gcaaccggcc tcattatgat
     1741 tttctcgttt tattttgtct gtgttcccgt aactggctgg aaggaccgac gccccaaaga
     1801 gaacggtccc cgcggaaaag ggggttcttt tccgcgggga ccgtttttat gcctctccgc
     1861 ccaggccgca ggcctcgatc acgtgctgtt ccatctcctt ggcgttgatt cttacgccgg
     1921 cgaaatactc cagggcgaag accgcctgat agatcagcat ccccaggccg ttggccgctt
     1981 tcagcccccg cagccgggcc tgccgcagca gctcggtctc ggccgggctg taaatgaggt
     2041 cgaacaccgc cgcgtgcccg ggcagttcgt ccaggaagct caggtcccgg aactggctgt
     2101 tcgtccctgt catgcccaga ctggtgcagt tgatgaggat gtcgc
//
LOCUS       DBMDSJ010000306         2143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11795_length_2143_cov_2.121450, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000306 DBMDSJ010000000
VERSION     DBMDSJ010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11795_length_2143_cov_2.121450"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="ACZPAQ_07415"
     CDS             <1..524
                     /locus_tag="ACZPAQ_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903854.1"
                     /translation="SAERMLGSGYESPIYTCIIRWGDDPAVVGEEYATIYGSYHCMTT
                     PLMTHVLTNASSSYPELYASPSCQAVSSMLNTYYKNFLWSGNPNGGELPQWDAWTSLD
                     GTTQLIVDANDDGAWAEMSTDHISYAEIIERMEADHSIPDDQKTEMIRTVLNGRWFSG
                     EMDAYYGNEDLWA"
     gene            complement(582..1043)
                     /locus_tag="ACZPAQ_07420"
     CDS             complement(582..1043)
                     /locus_tag="ACZPAQ_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903855.1"
                     /translation="MITLAQRIEALRTERGLSRPALSQALGFPKNAAEKFETGRQTPT
                     REQQEKMAAFFGVSLFYLRGESNDRTRQETWMDGGFQDEEEPAPPPRRAARPVPQSGP
                     AGQDQGTVFDAFLHSKAFQDMIRAAVQEALRSPEGQDILTQAVRKELARQR"
     gene            complement(1121..2002)
                     /locus_tag="ACZPAQ_07425"
     CDS             complement(1121..2002)
                     /locus_tag="ACZPAQ_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0903856.1"
                     /translation="MELYTLRYVLAVADSGNFSLAAQACHVGQPALSQQIAKLEKELG
                     VALFARNPRGATLTEAGKDFVLRAREILQRAQALQAEMSFYAGLRKGSLNLGIITSLQ
                     CINFGGMLSAFCGSYPDISVNIVQEGTHRLLELLLDRKVDLAFLNRPLSQLASSLEFV
                     KLGEDHYSLAVPRVHPLAGREDVSLRELRGEHFIFHQTGQVAAELCLTACRRAGFEPD
                     IVCRSSSPTTSLYMVQGGLGVAFLPSEDFRSHTLSGVREIKLEERIVKEVGVAWRRDA
                     ASPLVDAAVQFAREWVK"
BASE COUNT          420 a          642 c          708 g          373 t
ORIGIN      
        1 agtctgccga gcggatgctg ggcagcggct atgagtcccc aatctacacc tgtatcatcc
       61 gctggggcga tgatccggcg gtggtaggag aggagtatgc tacaatctat ggatcttatc
      121 attgcatgac cactccgctg atgacccatg tgctaaccaa tgcctcttcc agctatccgg
      181 agctctatgc ctcccccagc tgtcaggccg tctcctccat gctcaacact tattataaga
      241 atttcctgtg gagcggcaat ccaaatggtg gggaactgcc ccagtgggat gcctggacca
      301 gtctggacgg caccactcag ctgatcgtgg acgccaatga cgatggggct tgggccgaga
      361 tgtccaccga ccacatctcc tatgccgaga tcattgagag gatggaggcg gaccactcca
      421 ttcctgatga ccagaagacc gagatgatcc ggactgtcct gaatggtcgc tggttcagcg
      481 gcgaaatgga tgcatattac ggaaatgagg acctctgggc ctgaactgcc gtacaaaatg
      541 tcccgcgagc tctgctcgcg ggacattttt gtgtccggcg ctcaccgctg ccgggccagc
      601 tccttgcgga cggcctgggt gaggatatcc tgcccctcag gggagcgcag ggcctcctgt
      661 accgcggcgc ggatcatatc ctgaaacgcc ttgctgtgga ggaaggcgtc aaacaccgtg
      721 ccctggtcct ggccggcggg gccgctctgg gggacgggcc gggcggcgcg gcggggcggg
      781 ggagcgggct cctcctcatc ctggaagccg ccgtccatcc aggtctcctg acgggtgcgg
      841 tcgttgctct ccccccggag atagaagagg gagaccccga aaaaggcggc catcttctcc
      901 tgctgctccc gggtgggggt ctggcggccg gtctcgaact tttccgccgc atttttggga
      961 aagcccaggg cctgggagag ggcgggacgg ctgagacccc gttcggtgcg cagggcttcg
     1021 atgcgctgtg ccaatgtgat catagaaaag acctcctcag tggaatggct ctattataca
     1081 acaagaggga cagggggaaa agaggaaaag aagggggcgg tcatttgacc cactcccggg
     1141 cgaactggac cgcggcgtcc accagggggg aggccgcgtc ccggcgccag gccaccccca
     1201 cctccttgac gatccgctcc tccagtttga tctcccggac gccggagagg gtatgggagc
     1261 ggaagtcctc cgagggcaga aaggccaccc caagcccgcc ctggaccatg tagaggctgg
     1321 tggtgggact gctggagcgg cagacgatgt ccggctcaaa gcccgcccgg cggcaggcgg
     1381 tgaggcacag ctccgcggcc acctggccgg tctggtggaa gatgaagtgc tccccccgca
     1441 gctcccgcag gctgacgtcc tcacgaccgg ccagggggtg gacacggggg accgccaagg
     1501 agtaatggtc ctcccccagc ttgacgaact ccaggctgga ggccagctgg gacagggggc
     1561 ggttgagaaa ggccaagtcc accttccggt ccagcagcag ctccagcagc cggtgggtcc
     1621 cctcctgaac gatgtttacc gagatgtcgg ggtaactgcc gcagaaggcg gagagcatgc
     1681 cgccgaagtt gatgcactgg aggctggtga tgatgcccag gttgagggag cccttgcgca
     1741 gcccggcata gaaggacatc tccgcctgga gggcctgggc gcgctgaagg atctccctgg
     1801 cccggagaac aaaatccttg cccgcctccg tcagggtggc accccgggga ttgcgggcga
     1861 agagggccac ccccagctcc ttctccagct tggcgatctg ctgggacagg gcgggctgtc
     1921 ccacgtggca ggcctgggcc gccagggaaa aattccccga atcggccacg gccagcacat
     1981 agcgaagcgt gtaaagttcc atatggtttc atgcctccca gcgatgggtc ggccgccagt
     2041 ccccgcggcg ggcggagcgg gcagcaaaag cgttttttca gaccaacaaa atagagagca
     2101 tctgttttgt gcaaaatgtc aaatcaaaag gaaaagcggg ggc
//
LOCUS       DBMDSJ010000307         2143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11798_length_2143_cov_1.372674, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000307 DBMDSJ010000000
VERSION     DBMDSJ010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11798_length_2143_cov_1.372674"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="ACZPAQ_07430"
     CDS             <1..268
                     /locus_tag="ACZPAQ_07430"
                     /inference="COORDINATES: protein motif:HMM:NF022300.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HIR0903857.1"
                     /translation="ELSSVEDTKATLDIGGAYVRTYIPIGNSAITSIPLWELPGVLSG
                     KVTMKVTSDRLKVGDRVAMLPTADGQSYYVLTKVVRPGDIVPSD"
     gene            246..680
                     /locus_tag="ACZPAQ_07435"
     CDS             246..680
                     /locus_tag="ACZPAQ_07435"
                     /inference="COORDINATES: protein motif:HMM:NF022387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HIR0903858.1"
                     /translation="MTLFPLIEAPAEEAGQSSAVLPLYREIAWDFEQDRPIWKNGAPQ
                     WVTGAQAVATWVWNTLHYVRASLDLFSWSWGNELQALTGRPFSQAVKESEAARYIRDC
                     LVVNPYITDVQQVSVGFSGSTLSIRCAVQTIYGEVSVDVPEI"
     gene            664..1749
                     /locus_tag="ACZPAQ_07440"
     CDS             664..1749
                     /locus_tag="ACZPAQ_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR0903859.1"
                     /translation="MSLKFDELSQEVTPESIKAEMVQRLKEKGLDVDTREGSYTDLLF
                     SEAAYQIYKGLSYHPTLLAAAVPSEESGPYLDKFGGMFGLSRTPAATAHVLLTFSGED
                     GADIPQGTVAVSNGGLRFTTIQKAVISEGTAQATATAEQPGEEYNVAAGTVTRLATNL
                     YGVTGVTNKAPGEGGADQESDASFYNRVHTFLAEPVASGNANHYKQWARSVAGVGSAA
                     VIPLWDGNGTVKVIVASEDKEPLDETIVEQTAQYIESQRPIGASVTVVSAQALAIDVE
                     AACTLEGGVLPSAVQEELSQQITEMFHGMEMGASTPVRYNRIMAMLLSCSGVVDCSSM
                     TVNDGTVNIPVTTEQVPVLGTVTVTAG"
     gene            1763..>2143
                     /locus_tag="ACZPAQ_07445"
     CDS             1763..>2143
                     /locus_tag="ACZPAQ_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021583.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HIR0903860.1"
                     /translation="MELNRLERLPNRLRASPYTVGVAEPMTELALEYREATIDLADQF
                     FVPTATWGLKEWERLVGLSPDSGQTLEQRRSEVIAKLCSTGTTTAEMIRAMAEALTGY
                     SAGVTENFGEYSFSLRFYGEQAGFI"
BASE COUNT          403 a          649 c          733 g          358 t
ORIGIN      
        1 cgagctgtcc tcagtggagg acacaaaggc cacgctggac atcggcgggg cttatgtgcg
       61 gacctatata ccgataggaa acagtgctat aacgtccatc cccctgtggg agctgccggg
      121 cgttctctcc ggcaaggtga cgatgaaggt gaccagtgac cggctgaagg tgggagacag
      181 ggtggccatg ctgcccaccg ccgacggcca atcctactat gtgttgacaa aggtggtgag
      241 acccggtgac attgttccct ctgattgagg cccctgcgga ggaggccggc cagtcctcgg
      301 cggtcctgcc gctgtaccgg gagatcgcct gggactttga gcaggaccgg cccatctgga
      361 agaacggcgc cccccagtgg gtcaccgggg cccaggcggt ggccacgtgg gtgtggaaca
      421 ccctgcacta cgtccgggcg tccctggacc tgttcagctg gagttggggc aatgagctcc
      481 aggcgctgac cggccgcccc ttctcccagg cggtgaagga gtcggaggcg gcccgctata
      541 tccgggactg cctggtggtc aacccttata tcacagatgt gcagcaggtg tcggtgggct
      601 tttccggctc caccctgtcc atccgctgtg cggtacaaac catctatggg gaggtgagtg
      661 tggatgtccc tgaaatttga tgaactgtct caggaggtta cgcctgagag tatcaaggcg
      721 gaaatggtcc agcggctgaa ggaaaaaggg ctggatgtgg acacccggga ggggtcctac
      781 accgatctgc tgttttctga ggccgcctac cagatctaca aggggctgag ctaccacccg
      841 actctgctgg ccgcggcagt gcccagcgag gagtccggcc catacctgga caagttcggc
      901 gggatgttcg gcctgagccg gacgccggcg gccacagccc atgtgctgct gaccttcagc
      961 ggcgaagatg gggcggatat cccccagggg acggtggccg tgtccaacgg cgggctgcgc
     1021 ttcaccacca ttcaaaaggc ggtgatctct gagggcacgg cccaggctac cgccacggcg
     1081 gaacagcccg gagaggagta taacgtagcc gctggaacag tgacccggct ggccaccaat
     1141 ctgtacggcg tcaccggcgt caccaacaag gcgccgggcg agggcggagc cgatcaggag
     1201 agcgacgcct ccttttacaa ccgggtccat accttcctgg ccgagcctgt ggcctcgggc
     1261 aacgccaacc actacaagca gtgggcccgg tcggtggccg gcgtaggcag cgccgccgtg
     1321 atcccgctgt gggacggcaa tgggacagtg aaggtgatcg tggccagcga ggacaaggag
     1381 ccgctggacg aaaccattgt ggagcagacg gcccagtaca ttgagtctca gcgccccatc
     1441 ggagcctcgg tgactgtggt atccgcccag gccctggcca tcgatgtgga ggccgcctgc
     1501 acgcttgagg ggggcgtact gccctccgcg gtccaggagg agctgtccca gcagatcaca
     1561 gagatgttcc acgggatgga gatgggcgcc agtacgccgg tacgctacaa ccggatcatg
     1621 gccatgctgc tgtcctgcag cggggtggtg gactgctctt ccatgacggt caacgacggg
     1681 acggtgaaca tccccgtgac cacggagcag gtcccggtcc tggggaccgt gaccgtcacc
     1741 gccggctgag gggaggcgcg tcatggaact gaatcgactg gagcgcctgc caaaccgtct
     1801 gcgggccagc ccctacaccg tgggggtggc ggagcccatg acggagctgg cgctggagta
     1861 ccgggaggcc accatcgacc tggccgacca gttctttgta ccaacggcca cctggggcct
     1921 gaaagagtgg gagcggctgg tgggtctctc tccggacagt gggcagacgc tggagcagcg
     1981 ccgcagcgag gtgatcgcaa agctctgctc caccggcacc accaccgcag agatgatccg
     2041 ggccatggcg gaggcactga ccggctacag cgccggcgtg accgaaaatt tcggggaata
     2101 ctccttctcc ctgcgcttct acggagagca ggccggcttt atc
//
LOCUS       DBMDSJ010000308         2137 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11836_length_2137_cov_1.294204, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000308 DBMDSJ010000000
VERSION     DBMDSJ010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11836_length_2137_cov_1.294204"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="ACZPAQ_07450"
     CDS             complement(<1..588)
                     /locus_tag="ACZPAQ_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0903861.1"
                     /translation="MAKSPNQKLKLLYLYQILLQRTDEEHPITVPQLIGELDLRGIRA
                     ERKSVYDDLEALRLFGLDVQSRKGRSPGWFVGRREFELPELKLLVDVVQSSRFITRKK
                     SDALIRKLEGLASSHQARQLQRQVYVSGRVKAMNESIYYNVDKLHTALSARKSITFRY
                     FDYDMHRNKVFRREGRRYAVSPYGLIWNSENYYLVA"
     gene            complement(656..988)
                     /locus_tag="ACZPAQ_07455"
     CDS             complement(656..988)
                     /locus_tag="ACZPAQ_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR0903862.1"
                     /translation="MNIQIFGRSKCFDTKKAERWFKERRVRFQSVDVVKYGMSRGELS
                     SVKNAVGLEALADPKHPDYPLFQHLAYDQDKLDRLWEDQTMLRTPIVRNGRQATVGYC
                     PEVWAAWE"
     gene            1248..>2137
                     /locus_tag="ACZPAQ_07460"
     CDS             1248..>2137
                     /locus_tag="ACZPAQ_07460"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR0903863.1"
                     /translation="MKAVIMAGGEGTRLRPLSLGMPKPMVPLLGRPVMEHIIGLLKRH
                     GIDEIAVTLCYMPEAVQSYFGDGQKWGVRLHYFIEEAPLGTAGSVRACAPFLEGEDFL
                     VISGDSVCDLDLTAAMEFHQAKRAQATLVLFRHPAPLEYGLVLTDEEQRVVRFVEKPS
                     WGQVVTDLVNTGIYLLSPQVLEQIPPDRPWDFGKDVFPALLESGGGLYGVPGGGYWCD
                     MGDCGAYLDCAADALSGKVKLDMGLPQQSPGVWSTQPIPEGVTVIPPCWLGPGAAVEP
                     GALIGPHTVLEQNSWVGRRSL"
BASE COUNT          361 a          710 c          655 g          411 t
ORIGIN      
        1 agccaccaga tagtaattct cactgttcca gatcaggccg tagggagaga cggcgtaacg
       61 ccgcccctcc cgccgaaaga ccttgttccg gtgcatgtcg tagtcaaagt agcggaaggt
      121 gatgctcttc cgtgccgaga gggccgtgtg gagcttgtct acattataat atatgctctc
      181 gttcatggcc ttgacccggc cgctgacgta cacctgccgc tggagctggc gggcctggtg
      241 gctgctcgcc agcccctcca gcttgcggat cagggcgtcg ctcttcttcc gggtgatgaa
      301 gcgggaggac tgcaccacgt ccaccagcag cttcagctcc ggcagctcaa actcccgccg
      361 gcccacgaac cagcccgggg accggccctt ccggctctgc acgtccaggc cgaacagccg
      421 cagggcctcc agatcgtcgt agacgctctt ccgctccgcc cggatgcccc gcaggtccag
      481 ttccccgatc agctggggga cggtgatggg gtgctcctcg tcggtgcgct ggagcaggat
      541 ctgatacaga tacagcagtt tcagcttttg attgggactc ttcgccatgg gtcatcctcc
      601 tcattccggt aatgcactca gcataccgga acctgtgtcc catataacgg actattcact
      661 cccaggcggc ccacacctcg ggacagtagc ccaccgtggc ctgcctgccg tttcgcacga
      721 tgggggtgcg cagcatggtc tggtcctccc acagccggtc cagcttgtcc tggtcatagg
      781 ccagatgctg gaaaagagga tagtctgggt gcttggggtc ggccagggcc tccagcccca
      841 ccgcgttctt cacgctggac agctcccccc ggctcatgcc gtacttcacc acatccacag
      901 actgaaatct cacccggcgc tccttgaacc agcgctccgc ctttttggta tcaaagcatt
      961 tggacctgcc aaaaatctgt atgttcatta cgatatcctc cggaagcgcg ggccgcctcg
     1021 tcccctgccc ccgccgggca gggcggcctg catggtccct gtgtccttcc tcaggctgtt
     1081 aaattatgta tatctaagta taacatattc cctcccaggg cacaagtcaa aaccgctccg
     1141 gaaccggttt atttccccgg cggctggtca tactaggaga tgctacaaat acagggccgt
     1201 tcccgctccg ccccggcgga gcccggccgg aagggtggaa acacatcgtg aaagctgtca
     1261 ttatggcggg cggagagggg acccgcctgc gccccctgtc cctgggcatg ccgaagccca
     1321 tggtccctct gctgggccgg ccggtgatgg aacacatcat cggcctactc aagcgccacg
     1381 ggatcgacga gatcgccgtc accctgtgct acatgccgga ggcagtgcag tcctatttcg
     1441 gggacggaca gaagtggggg gtccgcctgc actacttcat cgaggaggcc ccgctgggca
     1501 ccgccggcag cgtgcgggcc tgcgccccct tcctggaggg agaggacttc ctagtcatca
     1561 gcggggacag cgtctgcgac ctggatctca ctgccgccat ggagttccat caggccaagc
     1621 gggcccaggc caccctggtc ctcttccgtc atcccgcgcc cctggagtac gggctggtcc
     1681 tcaccgacga ggagcagcgg gtggtccgct ttgtggagaa gccctcctgg gggcaggtgg
     1741 tgaccgatct ggtcaacacc ggcatctatc tgctctcccc ccaggtgctg gagcagatcc
     1801 ctccggacag gccctgggat tttggaaagg atgtgttccc agccctgctg gagtccggcg
     1861 gcggcctgta cggcgtcccc ggcggcggct actggtgcga catgggggac tgcggggcct
     1921 atctggactg tgcggcggac gccctctctg ggaaggtgaa gctggatatg ggcctgcctc
     1981 agcagtcccc cggcgtctgg agcacccagc ccatcccaga gggtgtcacc gtgatcccgc
     2041 cctgctggct gggcccgggg gcagcggtgg agccgggcgc cctgatcggc ccccataccg
     2101 tgctggagca gaacagctgg gtgggacgcc gcagcct
//
LOCUS       DBMDSJ010000309         2135 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11850_length_2135_cov_1.239430, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000309 DBMDSJ010000000
VERSION     DBMDSJ010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11850_length_2135_cov_1.239430"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="ACZPAQ_07465"
     CDS             complement(<1..264)
                     /locus_tag="ACZPAQ_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR0903864.1"
                     /translation="MKRWTMCLLLGLLLLLGSCSTAAEKGGYQLYFASGLEDGSAILS
                     EPYQGEADPGPRELMEALLSGPTEEELNSPFPSGVSLRSWGLEE"
     gene            complement(261..>782)
                     /locus_tag="ACZPAQ_07470"
     CDS             complement(261..>782)
                     /locus_tag="ACZPAQ_07470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903865.1"
                     /translation="ALTRLDSLPAAETFPVDVSAVTRRALSMLAPVADAAGVALGSNL
                     KDKCVVQGTEDDLYQICFNLLENGIKYNRPGGRVMADVYRDGDQVLLEVSDTGVGIPE
                     EDMPKVFNRFYRVDKARSRAAGGTGLGLSIVRDTVRRHGGWVTVRARQPEGSVFTVGF
                     PAVPEKRKEGDRG"
     assembly_gap    783..792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<793..1783)
                     /locus_tag="ACZPAQ_07475"
     CDS             complement(<793..1783)
                     /locus_tag="ACZPAQ_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR0903866.1"
                     /translation="MREEKKKKRGRVPFFSSIQMKYAMSYLAIVSVVLILLNTYPVLA
                     SQDLLFGSKRDSLKSQAAVMASALMELELLTSDQVERVMTMLDSSGLTRVVVTDPAGL
                     ILYDSTQPREKRGETAETAPVQEEPEEPQYRYALYQEVVLALGGNDVTCSEFSGQVFR
                     STAAVPIVYRNMTIGCVYICEEDAVQGALLLNLQKNLRSISLVVAAVTLVMSLFFAQM
                     LSSRFTALLSAIRIVGEGEYGHRLQPSGRDELALLAEEFNQLTDRLQTTEEVRRRFVS
                     DASHELKTPLASIRLLADSILQNETMDQVTLRDFVSDIGTEADRLTRITEHLLA"
     gene            complement(1819..>2135)
                     /locus_tag="ACZPAQ_07480"
     CDS             complement(1819..>2135)
                     /locus_tag="ACZPAQ_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024076.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HIR0903867.1"
                     /translation="VEYSGCSLAPGWNVKYRARGRALCTIYPDTGTFTCMISIGQKEA
                     PAMELVLPGLTAYVREVYQNSRPSRMGRWLMIRVSGPEILEDVKTLLRIRMPVRGAGS
                     KG"
BASE COUNT          371 a          738 c          627 g          389 t
ORIGIN      
        1 ctcctccagc ccccagctcc gcagggacac cccgctgggg aaggggctgt tcagctcctc
       61 ctccgtggga ccggagagca gagcctccat taattcccga gggcccgggt ccgcctcccc
      121 ctggtagggc tcgctgagga tggcgctgcc gtcctccaga ccggaggcga aatagagctg
      181 gtatccgccc ttctccgcgg cagtgctgca ggaccccagc aggagcagca gccccaggag
      241 caggcacatc gtccaccgct tcatccccgg tctccctcct tccgtttttc cggcacagcg
      301 ggaaagccca ccgtgaacac gctgccctcc ggctggcggg cccggacggt gacccatccc
      361 ccgtggcggc gcaccgtgtc ccggacaatg gacagcccca gcccggtgcc gccggcggcc
      421 cgggagcggg ccttgtccac ccggtagaac cggttgaaca ccttgggcat gtcctcctcc
      481 gggatgccca cccccgtgtc cgatacctcc agaaggacct ggtccccgtc ccggtacacg
      541 tccgccatca cccggccgcc tggccggttg tacttgatgc cgttctccag caggttgaag
      601 cagatctggt ataggtcgtc ctccgtcccc tggaccacac atttatcctt caaattggat
      661 cccagggcca ccccagccgc gtccgccacc ggcgccagca tggacagcgc ccgccgggtg
      721 accgccgaca cgtccaccgg gaaggtctct gccgccggca ggctgtccag ccgggtcagg
      781 gcnnnnnnnn nngggccagc aggtgctcgg tgatccgggt cagccggtct gcctctgtcc
      841 cgatgtcgga aacgaagtcc cgcagggtca cttggtccat ggtctcgttc tggagaatgg
      901 agtctgccag cagccggatg gaggccagag gggtcttcag ctcgtgggag gcgtcggata
      961 cgaagcgccg gcgcacctcc tccgtggtct ggagacggtc agtgagctgg ttaaactcct
     1021 ccgccagcag ggccagctcg tcccggcccg agggctggag ccggtgccca tactccccct
     1081 cccccacaat gcggatggcg ctgagcaggg cggtaaagcg ggaggagagc atttgggcga
     1141 aaaacaggct catcaccagg gtcaccgccg caaccaccag ggagatgctc cgcaaatttt
     1201 tttgtaagtt caacagcagc gcgccctgga ccgcgtcctc ctcacagata tagacgcagc
     1261 cgatggtcat attgcggtac acgatgggga ccgccgccgt ggaacggaac acctggccgc
     1321 tgaactcgga gcaggtcacg tcattccccc caagggccag cacaacctcc tgatagaggg
     1381 catagcggta ctggggctct tccggctcct cctggaccgg ggcggtctcc gctgtctccc
     1441 cacgcttctc cctgggctgg gtactgtcgt acaggatcag ccctgcgggg tcggtgacca
     1501 ctacccgggt caggccgctg ctgtccagca tggtcatgac ccgctccacc tggtccgagg
     1561 tgagcagctc cagctccatc agggcagagg ccatcaccgc agcctggctc ttcagcgaat
     1621 cccgcttgga gccaaacagc aggtcctggg aggccagcac cgggtaggtg ttcagcagaa
     1681 tcagcaccac tgacacgatg gccaggtagc tcatggcgta tttcatctgg atggaggaaa
     1741 aaaacggaac tctgcccctc tttttcttct tttcctcccg cacagcgctc cctccctttt
     1801 cccgcaagcc ggatattttc agcctttgct tcccgctccc cggaccggca tccggatccg
     1861 cagcaatgtt ttgacatcct ccaggatctc cggtcccgat acccggatca tcagccagcg
     1921 ccccattcgg ctgggtctgg aattctgata gacctcccgc acataggcgg tcagaccagg
     1981 gagcaccagt tccattgccg gggcttcttt ttgtccgatg gagatcatac aggtaaaggt
     2041 gccggtatcc ggatagatgg tacacagcgc tcgtcctctg gctcgatatt ttacattcca
     2101 gccgggggcc aggctgcacc cgctgtactc cacag
//
LOCUS       DBMDSJ010000310         2130 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11881_length_2130_cov_1.810744, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000310 DBMDSJ010000000
VERSION     DBMDSJ010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11881_length_2130_cov_1.810744"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="ACZPAQ_07485"
     CDS             <1..1160
                     /locus_tag="ACZPAQ_07485"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR0903868.1"
                     /translation="TGYLLLLTCVRCIHWFNPLVWNMVRTARRDMELCCDDDLLAGRD
                     QGARRAYGRAILDQMTAGKGGRTGLTTGFTGDRGGIFLRFRAIMDTTPKRRGRLLLAS
                     ASILILLAGGLVSCAPAAAESSGGALELSGADAGPGEMLPLEERFESYNRERGRSLPV
                     SRGEWAGEVRYRFADQEQGVEVLVGLSPGKGDPFPAEVLFQRRSFGFDMPVLPLAQGG
                     VEGSASLSLADLTGDGVPELVYIWGGGGTGAWEDNCRVFDLSTGEEYPILLDPELMLS
                     QVRAERLERREDHLIYQVTGPGGQSGEAALWCPESDQEPEGGPALGQISLIRMNETGD
                     ALEWTASLTLEGPPVLYGNSLGELTAVLEFSPSLRAFTAAPPWGMVLYPLE"
     gene            1269..>2130
                     /locus_tag="ACZPAQ_07490"
     CDS             1269..>2130
                     /locus_tag="ACZPAQ_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR0903869.1"
                     /translation="MRDGFIKVAAGTPKIRVADCRYNAEQIFTMMREADKQGVKVLCL
                     PELCLTGYTCGDLFLQDTLLCGAEEGLQTILEATRNLDMVTVLGLPVRCKWDNKLYNC
                     AAVIQSGEILGLVPKTYLPNYGEFYEQRWFASGAGVETSVDLCGETVDMDAAGLFACE
                     KVPNLVLGVEICEDLWATEPPSARLARGGATVILNLSASNELVGKSGYRRNLVVGQSG
                     RLVCGYVYADAGEGESTTDLVFTGHNMIAENGALLAERRFATGLTISEIDVDRLAYER
                     RRMTTFNGQRQ"
BASE COUNT          368 a          608 c          772 g          382 t
ORIGIN      
        1 atacaggcta tctgctcctg ctgacctgtg tccggtgcat ccattggttt aacccactgg
       61 tgtggaacat ggtccggacg gcccggcggg acatggagct gtgctgtgac gatgatctgc
      121 tggcgggccg ggaccagggg gcccgccggg cctacggccg ggcgatcctg gaccagatga
      181 ccgccggaaa aggggggagg accggtctga ctaccggctt taccggggac cggggaggga
      241 tctttctccg gtttcgggcc atcatggaca ccacgccaaa gaggagggga cgcctgctgc
      301 tggcctctgc ctccatcctg atcctcctgg ccgggggact ggtaagctgt gcgcccgccg
      361 cggcagagag cagcggaggc gcgttggaac tgtcgggagc ggacgcggga cccggggaga
      421 tgctgccctt ggaagagcgg tttgagtcct acaaccggga aagaggacgc tccctccctg
      481 tttcccgggg ggagtgggct ggagaggtcc gctaccgctt tgccgatcag gagcaggggg
      541 tggaggtgct ggtcggactc agcccgggga agggggaccc ctttcctgcg gaggtcctgt
      601 tccagaggcg gagctttggc ttcgacatgc cagtccttcc gctggcccag ggcggcgtgg
      661 aggggagtgc ctctttgtcc ctggccgacc tgacgggaga cggcgtcccg gagctggtgt
      721 acatctgggg cggcggcggg accggcgcct gggaggacaa ctgccgggta ttcgacctgt
      781 ctaccgggga ggagtacccc atcctcctgg acccggagct gatgctctcc caggtgcggg
      841 cggaacggct ggaacggcgg gaagatcatc tcatttatca ggtcaccggc cccggcggtc
      901 agtcggggga agcagccctg tggtgccccg agtctgacca ggagccggaa gggggaccag
      961 ccctggggca gatcagcctg atccggatga acgaaacggg ggatgcgctg gagtggaccg
     1021 cctccctgac gctggagggc ccgccggtcc tgtacgggaa cagcctgggt gagctgacgg
     1081 cggtgctgga gttctccccc tccctgcggg cgttcacagc ggcaccgccc tgggggatgg
     1141 tgctctaccc cctggaataa cacaaaattt ttaaggggag ggccctggag gggggccctc
     1201 cccttgtatt tgcccccaaa ttacggtatg atatcccttg atgcaaagac cacaaaggag
     1261 ggttttttat gcgggacgga tttatcaaag tggcagcggg caccccgaag atccgggtgg
     1321 cggactgccg ctataacgcg gaacagatct ttaccatgat gcgggaggcg gacaagcagg
     1381 gcgtcaaggt cctctgcctg ccggagctgt gcctcaccgg ctatacctgt ggggacctgt
     1441 ttttgcagga caccctgctg tgtggggcgg aggaggggct tcagaccatc ctggaggcca
     1501 ctcgaaactt ggatatggtg accgtgctgg gcctgccggt gcggtgcaaa tgggacaaca
     1561 agctgtataa ctgcgccgca gtcatccaga gcggcgagat cctgggactg gtgcccaaga
     1621 cctatctgcc caattacggg gagttttatg aacagcggtg gtttgcctcc ggagcgggtg
     1681 tggagaccag tgtggacctg tgcggggaga ccgtggacat ggacgccgcg gggctctttg
     1741 cctgtgagaa agtgcccaac ctggtcctgg gcgtggagat ctgtgaggac ctgtgggcca
     1801 ccgagccccc ctccgcccgg ctggcccggg gcggggctac ggtgatcctg aacctgtcgg
     1861 cctccaacga gctggtgggc aagtctggat accgccgcaa tctggtggtg gggcagtcag
     1921 ggcgcctggt gtgcggatat gtgtacgccg atgctgggga aggagagtcc accaccgacc
     1981 tggtgttcac cggccacaac atgattgccg agaacggtgc cctgctggcg gagcggcgct
     2041 ttgccacggg gctcaccatc agcgagatcg atgtggaccg gctggcctat gaacgccgcc
     2101 ggatgaccac cttcaacggc cagagacagg
//
LOCUS       DBMDSJ010000311         2124 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11934_length_2124_cov_1.818586, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000311 DBMDSJ010000000
VERSION     DBMDSJ010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11934_length_2124_cov_1.818586"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="ACZPAQ_07495"
     CDS             <1..266
                     /locus_tag="ACZPAQ_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR0903870.1"
                     /translation="ELARAHRCTLAVLKERSPSCGRGQIYDGSFTGRLTEGSGVTARL
                     LEEHGVQVYGESEAGTLAERGEAREEGQRASRPLPSKYPGKND"
     gene            686..>2124
                     /locus_tag="ACZPAQ_07500"
     CDS             686..>2124
                     /locus_tag="ACZPAQ_07500"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0903871.1"
                     /translation="MKEEHTLDQNKAPIYEALQTFRQMRVVPFDVPGHKRGRGNPELT
                     RFLGEQCVSIDVNSMKPLDNLCHPISVIREAEELAADAFGAAHAFLMVGGTTSSVQSM
                     VLSTCKRGDKIIMPRNVHRSVINALVLCGAIPVYVNPEVDPRLGISLGMKREQVARAI
                     RENPDAVAVLVNNPTYYGICSDLRAIVEMAHEAGMYCLADEAHGTHFYFGDHMPVSAM
                     EAGADMASVSMHKSGGSLTQSSLLLIGPGIHEGHVRKIINLTQTTSGSYLLMSSLDIS
                     RRNLALRGREVFRQVCAMAEYAREEINAVGGYYAFGRELINGDSIFDFDPTKLSVHTR
                     DVGLAGIEVYDLLRDEYDIQIEFGDIGNILAYLSIGDRPQELERLVSALAEIRRRYQT
                     DGSGLMNQEYIAPEVVTSPQEAFYADKRSVRLEDSVGQVCSEFVMCYPPGIPILAPGE
                     RITAEILDYIRYAKEKGCSMTGPEDPEIE"
BASE COUNT          425 a          646 c          670 g          383 t
ORIGIN      
        1 tggagctggc ccgggcccat aggtgcaccc tggcggtgct gaaggagagg agcccctcct
       61 gcggcagggg gcagatctat gacggcagct tcaccggcag gctgactgag ggctccggtg
      121 tgaccgcccg cctgctggag gaacacgggg tccaagtgta cggagagtca gaggccggca
      181 cattggcgga gcgcggggag gcgcgggagg aggggcaaag ggcgtcccgc cctttgccct
      241 ccaaatatcc cgggaaaaac gattgacata tcggaagttc tatgctatat tttgtagcaa
      301 tctgactgga gtttatctgg ccaagaatac tgggaccact cctccgggag tcaaggccat
      361 acggacagcc gggtccattg ccgatgggcg ggtcaccgcc cttattgatt gagttaaaag
      421 ctcaaatttt ataagttggt tacttcataa ccagatgcgt gtttgcgcag acttgtgaaa
      481 cggtcaataa cagagtggta aaagtatctt gttgctatat agactctcag taccagacat
      541 atctcccgcc cccagctgtt atttgcaggc gtttgtccgc catcgcggcc ggacagggga
      601 cttggagctt atcgtgtaca aattgatcca aacacaagct gttcaaatgc agcttgtgtt
      661 tttttgtgct tcgaggtgtt ggcagatgaa agaagaacat accctggacc agaacaaggc
      721 cccgatctac gaggcgctcc agaccttccg gcagatgcgg gtcgttccct tcgatgtgcc
      781 cgggcacaag cggggccggg gcaacccgga gctgacccgc ttcctggggg agcagtgtgt
      841 cagcatcgac gtgaacagca tgaaacccct ggacaacctg tgccacccca tctctgtcat
      901 ccgggaggcg gaggagctgg ccgccgacgc cttcggcgcg gcccacgcct ttctgatggt
      961 ggggggcacc accagctcgg tgcagagcat ggtgctgtcc acctgcaagc ggggggacaa
     1021 gatcatcatg ccccggaatg tccaccgcag cgtcatcaac gccctggtgc tttgcggtgc
     1081 catccctgtc tatgtcaacc cggaggtgga cccgcgcctg ggcatctccc tgggcatgaa
     1141 gcgggagcag gtggcccgtg ctatccggga gaacccagac gctgtggcgg tgctggtgaa
     1201 caaccccacc tactacggca tttgcagcga cctgcgggcc atcgtggaaa tggcccacga
     1261 ggcgggcatg tactgcttgg cggacgaggc ccacggcacc cacttctact tcggggacca
     1321 catgccggtg tcagcgatgg aagccggggc ggacatggcc tccgtgtcca tgcacaagag
     1381 cggcgggagc ctgacccagt ccagcctcct gctcatcggc cccggcatcc acgaggggca
     1441 tgtgcggaag atcatcaacc tgactcagac cacctcgggc agctatctgc tgatgtccag
     1501 cctggatatc agccgccgca acctggccct ccgcggccgg gaggtctttc gccaggtgtg
     1561 cgccatggcg gagtacgccc gggaggagat caacgccgtg ggcgggtact acgccttcgg
     1621 ccgggagctc atcaacggcg actccatctt cgacttcgac cccaccaagc tcagcgtcca
     1681 cacccgggac gtgggcctgg ccggcatcga ggtctacgac ctgctccggg acgagtacga
     1741 catccagatc gagttcgggg atatcggcaa catcctggcc tatctgtcca tcggggaccg
     1801 gccccaggag ctggagcggc tggtcagcgc cctggcggag atccgccgcc gctatcaaac
     1861 cgacggcagc ggcctgatga accaggaata catcgccccc gaggtggtca ccagccccca
     1921 ggaggccttc tacgcggaca agcgcagcgt ccgcctggag gacagtgtgg gacaggtgtg
     1981 cagcgaattc gttatgtgct atccccccgg catccccatc ctggcccctg gggagcggat
     2041 cacggcggag atcctggact acatccgcta cgccaaggag aagggctgct ccatgaccgg
     2101 acccgaggac ccggagatcg agga
//
LOCUS       DBMDSJ010000312         2122 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11940_length_2122_cov_1.563088, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000312 DBMDSJ010000000
VERSION     DBMDSJ010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11940_length_2122_cov_1.563088"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="ACZPAQ_07505"
     CDS             <1..885
                     /locus_tag="ACZPAQ_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0903872.1"
                     /translation="ELVSGWLVSRSETASAETERYTVDCTVEVVPSQEALAPFVSLRH
                     RTDSVPVLLPDDGVVLTEKLASLLGVSAGDTFTLDGDSRVQVRVADVTEHYIMHYVYM
                     TDAYYQTLYGQPPEENQVLAAYPAEGAEALDAELVGLDGVTTLSQVAATREVFTSSLE
                     SVDYAVVLIIVCAAALAFVVLYNLTNINITERMRELATLKVLGFYDGELSAYIYRENV
                     ILTVFGVALGMGMGKLLHQWLVLTVEIDLLMFGRTLALSSYLYAGVLTAVFSLVVNLA
                     AHRKLKKLDMVESLKTVE"
     gene            complement(1175..>2122)
                     /locus_tag="ACZPAQ_07510"
     CDS             complement(1175..>2122)
                     /locus_tag="ACZPAQ_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR0903873.1"
                     /translation="VPLVFPAGESGVFLSPLLAREGECPWIYTRGITAALDFVGMSFD
                     EAHARVREAARCLDGTTVRSKEALDQLLADRVGEQLPPEQRALWDSPSMYGRPDRQTV
                     GGAAVSFLLRPCSFASLVVFGRREENSPTFTSFRRWLGREPSPCPQGEAELVRRFLHC
                     YGPAAPADLQAWLGSSPQQAKRLWSAAAEEMEPVRVLGRTRWALIRDRDSLTAPPEQP
                     GRLILLGPHDPYLDLRDRALVLEDPARQRQVWRTVSNPGAVLKDGRVAGIWTGKAVQG
                     RLEFSLSLWEPLSPGARRALEHEAGAYAAFRGLELSLLP"
BASE COUNT          366 a          676 c          760 g          320 t
ORIGIN      
        1 gagctggtga gcggatggct ggtgagccgc tcggagacgg cctccgccga gacggagcgg
       61 tacacggtgg actgcacggt ggaagtggtg cccagccagg aggcgctggc ccccttcgtc
      121 tccctgcgcc accggacgga cagcgtgccg gtgctcctgc ctgacgacgg agtggtcctg
      181 acggagaagc tggcctccct gctgggggtg tcggcggggg acaccttcac tctggacggg
      241 gacagccggg tccaggtacg ggtggcggat gtcacggagc actatatcat gcactatgtc
      301 tacatgaccg acgcctacta ccagaccctg tatggccagc cgccggagga gaaccaggtg
      361 ctggcggcct accccgctga aggggcggag gccctggacg cggagctggt ggggctggac
      421 ggcgtcacca ccctgtccca ggtggcggcc acccgggagg tattcacctc cagtctggag
      481 agcgtggact acgccgtggt cctcatcatc gtgtgcgccg cggccctggc cttcgtggtg
      541 ctctacaacc tgaccaacat caacatcacc gagcggatgc gggagctggc caccctgaag
      601 gtgctgggct tctacgacgg cgagctgtcc gcctatatct accgggagaa cgtgatcctc
      661 acggtgttcg gcgtggccct ggggatgggg atggggaagc tgctccacca gtggctggtg
      721 ctgacagtgg agatcgacct gctcatgttc gggcggacac tggccctgtc cagctatctc
      781 tacgccgggg tactcaccgc cgtcttctcc ctggtggtca atctggcggc ccaccggaag
      841 ctgaaaaagc tggatatggt agagtcgctg aagacagtgg aatgaacagc agcagcgccg
      901 cccgatgggc ggcgctgaga gggtcgaaaa agtggcaagg ccactttttc gagaaagatg
      961 cagtctgccg cgcgcagggt ttgtccgccg caggcggaca aaccccacac ttgcagcctg
     1021 agaggtgttt tccccgacgc acatgtcgcc ggggaaaaca gatcagaact ggattcgcgc
     1081 ctgcgggcgc gaactttgcg aggcttgttc gtaaactgat tagcaccgcc cgtagggcac
     1141 agctgctcag cttgcggatc aggcggcccc gcgattaggg caggagactc agctccaggc
     1201 cccggaaggc ggcgtaggcc cccgcctcat gctccagagc ccgacgcgct ccaggagaga
     1261 ggggctccca cagggacagg gagaactcta gtcgtccctg taccgctttt ccggtccaga
     1321 tgccggccac gcgcccatcc ttcagaacgg cgccggggtt ggagactgtc cgccagacct
     1381 ggcgctggcg ggcggggtcc tccaggacca gggctcggtc ccgcaggtcc agataggggt
     1441 cgtggggccc cagcaggatc aggcggccgg gctgctccgg cggagcggtg aggctgtccc
     1501 ggtcccggat cagggcccag cgggtccggc ccaacacccg gaccggttcc atctcctctg
     1561 cggcggcgga ccagagccgc ttcgcctgtt ggggagagct gcccagccag gcctggagat
     1621 cggcgggggc ggcggggccg tagcagtgga gaaatctccg caccagctcc gcctccccct
     1681 gagggcaggg ggagggctcc cggcccagcc agcggcggaa ggaggtaaag gtgggactgt
     1741 tctcctcccg gcggccgaac accaccagag aggcaaagga gcaggggcgg aggagaaagg
     1801 agacagcggc gcctcccacg gtctgcctgt cagggcggcc gtacatggag ggggagtccc
     1861 acagggcccg ctgttccggc gggagctgct ccccgacccg gtcagccagc agctggtcca
     1921 gggcctcctt gctgcggacg gtggtcccgt ccaggcagcg ggcggcctcc cgcacccggg
     1981 cgtgggcctc gtcgaaggac atgccgacaa agtccagggc cgcggtgatc ccccgggtgt
     2041 agatccaggg acactccccc tcccgggcca gaagggggga gagaaagacg ccgctctccc
     2101 cggcgggaaa gaccaggggc ac
//
LOCUS       DBMDSJ010000313         2120 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_11957_length_2120_cov_1.571570, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000313 DBMDSJ010000000
VERSION     DBMDSJ010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11957_length_2120_cov_1.571570"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="ACZPAQ_07515"
     CDS             <1..793
                     /locus_tag="ACZPAQ_07515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR0903874.1"
                     /translation="ELYTKSTAGELALAADMAGCALIGRPLVEGTGSLANFRVQAKNL
                     GTDLMGAYLAAAQELVQRLDTFTFPQKERPELLVLHASSHHTSNTMALWAGVRERLGE
                     VCSVQEIGLRNGTLDDCSGCPYTMCIHFGEKGECFYGGVMSREVYPAVRRADGVVMLC
                     PNYNDALSANLTAFINRLTALFRQTRFYDKALFALVVSGYSGSDLVARQLISAMNMNK
                     SFYLPGRFALLETANDPGEAMGLPGVRDRLDRFADHMLEVLARRA"
     gene            complement(903..1661)
                     /locus_tag="ACZPAQ_07520"
     CDS             complement(903..1661)
                     /locus_tag="ACZPAQ_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR0903875.1"
                     /translation="MKRTVFQKLGDFVLGKGFYIVLFLCVATIGISGYYLVRTVSSTP
                     GEAEPVTGSPTVVLPDSSQNAAPPHTTPSVTTPTTPTVTEPEDSGSQEASLPQTVEEE
                     PAETVQPVEEDPVPAVFTWPVKGEILSGFSLEVLAYDETMGDWRTHSGVDIAASVGTN
                     VLAISAGTVQGVYDDDLMGTCVVVDHGDGLTSTYCNLSAKPTVSEGDTVSTGTVLGVV
                     GETAIAESSRPSHLHLEMAQDGKAVDPVSYLPQR"
     gene            complement(1795..>2120)
                     /locus_tag="ACZPAQ_07525"
     CDS             complement(1795..>2120)
                     /locus_tag="ACZPAQ_07525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0903876.1"
                     /translation="LDVMGNQNSLALGLFSEDVLIGISLGRLRHWYNGIEYCIDDLCI
                     ASEHQGQGFGSLWIQLIRNYACQHGFRNISLQTKRNAPAYHFYQKTGFEEQEDRVCFS
                     LDCLP"
BASE COUNT          402 a          641 c          673 g          404 t
ORIGIN      
        1 agagctgtac accaaatcca ccgccggaga gctggcccta gccgcagata tggcgggatg
       61 cgccctcatc gggcggccgc tggtggaggg gacgggctcc ctggccaact tccgcgtcca
      121 ggccaaaaac ctgggcaccg acctgatggg ggcctatctg gcggcggcac aggagctggt
      181 ccagcgtctg gacaccttca ctttccccca aaaggagcgg cctgagctgc tggtcctcca
      241 cgcctccagc caccacacat ccaacaccat ggccctgtgg gcaggggtgc gggagcggct
      301 gggggaggtg tgcagtgtcc aggagatcgg cctgcgcaac gggacactgg acgactgctc
      361 cggctgtccc tacaccatgt gtatccactt cggggagaag ggggagtgct tctatggcgg
      421 agtcatgtcc cgggaggtct atcccgcggt gcgccgggcc gacggggtgg tcatgctgtg
      481 ccccaactac aacgacgcac tctccgctaa tctcactgcc ttcatcaacc gcctcaccgc
      541 cctgttccgc cagacccgct tctatgacaa ggccctgttc gccctggtgg tctctggcta
      601 ttcgggcagc gacttggtgg cccggcagct gatctcggcc atgaacatga acaagtcctt
      661 ctatctgcca ggacgctttg ccctgctgga gacagccaat gacccagggg aggccatggg
      721 gctgcccgga gtccgggatc ggttggaccg gttcgcggac catatgctgg aagtcctggc
      781 ccggcgggct tgaagagggc ccttctccct cggtgaaagg catggcgggg cgggggagaa
      841 gattattccg cagacagcaa agggctccac acccatcaag ggcgtggagc tcttttgatc
      901 cttcagcgct ggggcaggta gctcacaggg tccaccgcct tgccgtcctg ggccatctcc
      961 agatggagat gggaggggcg gctgctctcg gcgatggcgg tctcacccac cacgcccagc
     1021 acggtgccgg tgctgacggt gtcgccctcc gaaacagttg gcttggcgct gaggttgcag
     1081 taggtgctgg tcaggccgtc cccgtggtcc actaccacgc aggtgcccat caggtcgtca
     1141 tcgtagactc cctggaccgt gcccgcgctg atggccagca cgttggtgcc cacgctggcg
     1201 gcgatgtcca cgccgctgtg ggtgcgccag tcgcccatgg tctcatcata ggccagcacc
     1261 tccaggctga agccggagag gatctccccc tttaccggcc aggtgaagac agcgggaaca
     1321 gggtcctcct ccaccggctg gaccgtctcg gccggctcct cctccacggt ctgggggagg
     1381 gaagcctcct ggctcccgct gtcctctggc tcggtcacag taggcgtggt gggtgtagtg
     1441 acagagggag tggtatgggg gggagccgca ttctggctgc tgtcgggaag gaccacggtg
     1501 gggctgccgg tgaccggttc cgcctctccg ggggtgctgc tgacggtgcg aacaaggtaa
     1561 tagcccgaaa ttccaatggt ggcgacacag aggaaaagga ctatgtagaa gccctttccc
     1621 agtacgaagt cgcccagttt ctgaaatacg gttcgtttca tggaaccacc tccgaagcct
     1681 attgtggaca ggcccgggga tggttataca ttccagagaa aaaattttat ggcacttctg
     1741 ccctctgagc aaaaatcgac cgcgcgatga gcgcggcggg ggactgccct cagatcaggg
     1801 aaggcagtcc agagaaaagc agacccggtc ctcctgctct tcaaagccgg ttttttgata
     1861 aaagtggtaa gcgggcgcgt ttcttttggt ctggagcgag atgttccgaa agccgtgctg
     1921 acaggcataa tttcggatca gctggatcca cagggacccg aacccttgtc cctgatgttc
     1981 tgaggcaatg cacaggtcgt caatgcagta ttcaatgcca ttgtaccagt gccgcagacg
     2041 gcccagagag atcccaatca ggacatcttc cgaaaaaagt cccagggcca aagaattctg
     2101 attgcccatc acgtccagaa
//
LOCUS       DBMDSJ010000314         2104 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12049_length_2104_cov_1.915627, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000314 DBMDSJ010000000
VERSION     DBMDSJ010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12049_length_2104_cov_1.915627"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="ACZPAQ_07530"
     CDS             complement(<1..299)
                     /locus_tag="ACZPAQ_07530"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR0903877.1"
                     /translation="MDLIVAVDQNWAIGKDGEQLLYLSQDLKRFKALTLGHAVILGRR
                     TLSTFPGGRPLKGRRNLILSTTPGFAPEGGEVYPSLDALLAAAPADAFVIGGESRS"
     gene            complement(315..1145)
                     /gene="thyA"
                     /locus_tag="ACZPAQ_07535"
     CDS             complement(315..1145)
                     /gene="thyA"
                     /locus_tag="ACZPAQ_07535"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR0903878.1"
                     /translation="MSYADQVFIQNCQDILSRGVWDTDREVRPRWEDGTPAHTIKLFG
                     VVNRYDLRREFPILTVRRQFLKSAVDELLWIWQKKSNNVHDLNSRIWDAWADESGSIG
                     KAYGYQLGVKHHYPQGDMDQVDKVLWDLKHDPGSRRILTSLYNHHDLSEMALYPCAYS
                     MTFNVSGNTLNGILNQRSQDMLTANGWNVMQYAVLLHMVAQVSGLEAGELLHVIADAH
                     IYDRHVDAVREIITRQPYDAPRFSLDPAVTDFYAFTPDSVRLEGYQAHPVPGTIPVAV
                     "
     gene            complement(1289..1537)
                     /gene="spoIIID"
                     /locus_tag="ACZPAQ_07540"
     CDS             complement(1289..1537)
                     /gene="spoIIID"
                     /locus_tag="ACZPAQ_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HIR0903879.1"
                     /translation="MKGNIEERACQLALYIIENRTTVRAAAQKFGISKSTVHKDLSER
                     LPAFNQPLYLQVKGVLEENKAERHIRGGIATRRKYKGA"
     gene            complement(1662..>2104)
                     /locus_tag="ACZPAQ_07545"
     CDS             complement(1662..>2104)
                     /locus_tag="ACZPAQ_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR0903880.1"
                     /translation="IIPDEVVLEGTIRTLEDGLRQYAARRIGEVAQAAAAVFGASADC
                     TITWGAPPVLNDPAMAELAAGAAAEVVGPEHVVTRIPAPNMGGEDFAFYLEQVPGAFL
                     FLSSSDPAKGTDLPHHSPRFDVDESVLWKGAALYVSLAQTFLNG"
BASE COUNT          384 a          617 c          709 g          394 t
ORIGIN      
        1 gatctggact ccccgccgat gacaaaggca tccgccggag cggcggcgag gagggcgtcc
       61 agggagggat agacctcacc gccctccggg gcgaagcccg gcgtggtgga caggatcagg
      121 ttgcgccgtc ccttcagggg gcgtccgccg ggaaaggtag agagggtcct gcggcccaga
      181 atgaccgcgt ggcccagggt cagggccttg aagcgcttca ggtcctggga gagatagaga
      241 agctgctctc cgtccttccc gatggcccag ttctggtcca ctgctacaat aagatccata
      301 ctgtgcgcct cctcttagac cgccaccggg atcgttcccg gcacagggtg ggcctgatac
      361 ccctccaggc gcacgctgtc cggggtgaag gcgtagaaat cggtgacagc ggggtccagg
      421 gagaatcggg gggcgtcata gggctggcga gtgatgatct cccgcaccgc gtccacgtgg
      481 cggtcgtaga tatgggcgtc ggcgatgacg tggagcagtt cgccggcctc cagcccggag
      541 acctgggcga ccatgtggag cagaacggcg tactgcatca cgttccagcc gttggcggtg
      601 agcatgtcct ggctgcgctg gttcaaaatg ccgttcaggg tgttcccgct gacgttgaag
      661 gtcatggaat aggcacaggg gtagagggcc atctcgctca ggtcgtggtg gttgtagagg
      721 ctggtgagga tgcggcggga gccggggtcg tgcttcaggt cccacagcac cttgtccacc
      781 tggtccatgt ccccctgggg gtagtggtgt ttcaccccga gctggtagcc gtaggccttg
      841 ccgatggagc cggactcgtc cgcccaggcg tcccagatgc ggctgttcag gtcatggacg
      901 ttgttggact tcttctgcca gatccacaga agctcgtcca ccgcggactt caaaaactgc
      961 ctgcggacgg tgagaatggg gaattctctg cgcaggtcat accggttgac caccccaaag
     1021 agcttgatgg tatgggcggg ggtcccgtcc tcccagcggg gacggacctc ccggtcggtg
     1081 tcccacactc cccgggaaag aatgtcctga cagttttgaa taaagacctg atcggcgtag
     1141 ctcatggcgg cccctcctga tattggtttg aaggtatcat atcagaagcg gggggaaaaa
     1201 gcaataaggc ggggacgcgg catcctgccg cgtccccgcc gcctgttccg gaagccggac
     1261 gccaggagga aagcgcccct cccccggctc aggctccttt gtatttccgt cgggtggcga
     1321 tgccgccccg gatatgccgt tccgctttgt tctcctccag cacgcccttg acctggagat
     1381 agagaggctg gttgaaggcg ggaaggcgct cggacaggtc cttatggacc gtggacttgc
     1441 tgatcccgaa cttctgtgcc gccgcgcgga ccgtggtccg gttctctatg atgtaaaggg
     1501 ccagctgaca ggcccgttcc tcgatattcc ctttcacaca gcaacaactc ccccggattt
     1561 tgcatctgct gtacatgtat atgcggggga gaggatttta tgaggccgga aatgcctcaa
     1621 ctggaagaat ttcccgatga aggcccagtc gtggagagga ttcagccgtt gagaaaggtc
     1681 tgagcaaggg agacgtagag ggcggcccct ttccagagca ctgactcgtc cacgtcgaac
     1741 cggggactat gatggggaag gtccgtcccc ttggccgggt cggaggaact gagaaagaga
     1801 aaagccccgg ggacctgctc cagatagaag gcgaaatcct cacctcccat gttgggggct
     1861 gggatgcggg tgacgacgtg ctccgggccc acgacttcgg cggcggcccc ggcggccagc
     1921 tccgccatag cgggatcgtt gaggacaggg ggagcgcccc aggtgatggt gcagtccgca
     1981 ctggcgccga atacggcagc cgctgcctgg gccacctcac cgatgcgccg ggcggcgtac
     2041 tgccgcagac cgtcctccag agtgcggatg gtcccctcca gcactacctc gtccgggatg
     2101 atgt
//
LOCUS       DBMDSJ010000315         2103 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12056_length_2103_cov_1.806194, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000315 DBMDSJ010000000
VERSION     DBMDSJ010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12056_length_2103_cov_1.806194"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="ACZPAQ_07550"
     CDS             <1..641
                     /locus_tag="ACZPAQ_07550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0903881.1"
                     /translation="TSAHDIALMSRELILNHPSIREYTTIWMDSIRDGAFGLTNTNRL
                     VRFYQGATGLKTGSTDSALYCVSATAERDGMELIAVVMKSPTSAQRFEDAKALLDYGF
                     ANYTLARVYPDVPLAPVEVLLGTTAQVQPQLERECSLLVRKGEEGLISTQIQLAQDLE
                     APVERGQKLGQMVVTVDGEVRDTIPIVASQQVDRLTIPGIFGKMFQRLLMAR"
     gene            823..>2103
                     /gene="glgB"
                     /locus_tag="ACZPAQ_07555"
     CDS             823..>2103
                     /gene="glgB"
                     /locus_tag="ACZPAQ_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR0903882.1"
                     /translation="MAKTKKNAPVYAPEHELRAFSDGHSTHAWECFGAHAGEQDGVPG
                     YYFRVWAPNAQQVCVFGYFNDWDPNANPLTPVQGGIWEGFIPGLQQYDAYKYAVHTKD
                     GRLTGKADPFAFHAETRPGTASKLYDLSGYQWGDRAWLDYRAKNAPYRRPMNIYECHL
                     GSWRRTGEGEFLSYRDLAAYLVPYVKELGFTHVELLPVTEHPLDASWGYQCTGYFAAT
                     SRFGTPHDLMYFVDQMHQAGIGVIMDWVPAHFPRDEFGLYHFDGTPTYEYADPRKGEH
                     ADWGTNVFDYGRNEVRSFLYSSAMFWLDEFHFDGLRVDAVSSMLYLDYARKPGEWIPN
                     IHGGKENLEAIEFLQRLNEMVFGAHPDVLMIAEESTAWPKVTYPVSDGGLGFNFKWNM
                     GWMNDICHYIKMDPYFRQFNHRDITFSLVYAFSEN"
BASE COUNT          396 a          690 c          578 g          439 t
ORIGIN      
        1 tcacctccgc tcacgacatc gccctcatgt cccgggagct gatcctcaac catcccagca
       61 tccgggagta caccaccatc tggatggact ctatccggga cggtgccttc ggtctcacaa
      121 acaccaaccg tctggtccgc ttctatcagg gagccaccgg cctcaagacc ggttccaccg
      181 attccgccct gtactgcgtc tccgccaccg ccgagcggga cggcatggaa ctcatcgccg
      241 tggtgatgaa gtctcccacc tccgctcagc gctttgaaga cgccaaagcc cttctggatt
      301 acggcttcgc caactatacg ctcgcccggg tctatcccga tgtcccactg gcccccgtgg
      361 aggtgctgct gggcaccacc gcccaggtcc agcctcagtt ggagcgggag tgttccctcc
      421 tggtccggaa gggggaggag ggcctgatct ccacccagat ccagctcgcc caggacctgg
      481 aggccccggt ggagcggggg cagaaactgg ggcagatggt ggtcactgtg gacggcgagg
      541 tccgggacac catccccatc gtcgcctccc agcaggtgga ccgcctcacc attcccggca
      601 tctttggaaa gatgtttcag cggctgctga tggcccgctg acgccctgtt cggagctccc
      661 cggccgccgg ggggctccga ataaattcca ttttaccggc ttttcacttg acgcgtcctc
      721 tcttctgttg tataattttt ataaatatag aggagaattt ttggtttttg ttcgtgcttt
      781 tcgaccattt gtgacacaaa agagtgattt gaggtggttt cattggccaa aacgaagaaa
      841 aatgccccgg tttatgcccc ggaacatgaa ctgagagcgt tcagtgatgg acacagcacc
      901 catgcctggg agtgcttcgg cgcccatgcc ggcgagcagg acggtgtccc cggctactat
      961 ttccgagtct gggcccccaa cgcccagcag gtgtgtgtct ttggttattt caacgactgg
     1021 gaccccaacg ccaaccctct gacccctgtt cagggcggca tctgggaggg gttcatcccc
     1081 ggccttcagc agtatgacgc gtataaatat gcagtacata ccaaggatgg ccgcctgacc
     1141 ggcaaggctg acccctttgc cttccacgca gagactcgcc caggcaccgc ctccaaactt
     1201 tacgacctct ccggttacca gtggggggac cgggcctggc tggactatcg ggccaaaaac
     1261 gccccctacc gccgccctat gaacatctat gaatgtcacc tgggctcctg gcgccgcacg
     1321 ggggaagggg agttcctcag ctaccgggat ctggccgcct atctggtccc ctatgtgaag
     1381 gagctgggct tcacccatgt ggagctcctc cctgtcaccg aacatcccct ggatgcctcc
     1441 tggggttacc agtgtaccgg ctactttgcc gccaccagcc gcttcggcac cccccacgac
     1501 ctgatgtatt tcgtggacca gatgcaccag gctggcatcg gcgtcatcat ggactgggtc
     1561 cccgcccact tcccccggga tgagtttggc ctctatcact ttgacggcac ccccacctac
     1621 gagtatgccg acccccggaa gggagagcac gccgactggg gcaccaacgt ctttgactac
     1681 ggacgcaacg aggtgcgctc cttcctctac tcctccgcca tgttttggct ggatgagttc
     1741 cattttgacg gcctgcgggt ggatgcggtc tcctccatgc tctacctgga ctatgcccgc
     1801 aagcccggag agtggatacc caacatccac ggcggtaagg agaacctgga ggccatcgag
     1861 ttcctccagc gcctcaacga gatggtcttc ggtgcccatc ccgacgtgct gatgatcgcg
     1921 gaggagtcca ccgcctggcc caaggtcacc tatccggtga gcgacggggg cctgggcttc
     1981 aacttcaagt ggaacatggg ctggatgaac gacatctgcc actacatcaa aatggaccca
     2041 tacttccggc agttcaacca ccgggacatc accttctccc tggtgtatgc cttctcggag
     2101 aac
//
LOCUS       DBMDSJ010000316         2098 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12080_length_2098_cov_1.370055, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000316 DBMDSJ010000000
VERSION     DBMDSJ010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12080_length_2098_cov_1.370055"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            56..652
                     /locus_tag="ACZPAQ_07560"
     CDS             56..652
                     /locus_tag="ACZPAQ_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR0903883.1"
                     /translation="MKPAAPAAPAAAPAPRAPFAGPVYYAAVPVPQTPGYVSRAIQPF
                     AASYQPSYLKMGDREDCTGNFTITIDGKPYQVSVAKADTPTAAPVAAAPVAAAAPAAA
                     APVAAPTPAPAAAPAAAPAPTAVAAGETPVNSPMPGNIFKVECKPGQAVKAGDVLVVL
                     EAMKMEIEVSAPVDGTVKSVSATVGTAVNTDDLLVVLG"
     gene            866..1723
                     /gene="rfbA"
                     /locus_tag="ACZPAQ_07565"
     CDS             866..1723
                     /gene="rfbA"
                     /locus_tag="ACZPAQ_07565"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR0903884.1"
                     /translation="MKGIVLAAGKGTRLYPMTKPVCKPLLPVYDKPLIYYPIAILMQA
                     GISEILVIVPPNETATFTALLGDGSQYGLKISYAEQPVPRGIADALLIGQEFVGDDRV
                     CLVLGDNIFYAPTLGATLKKAAASPAAATVFGYYVEDPRPFGVVEFDKNGKAISIEEK
                     PRYPKSNYIVPGLYFYDQQVMEIARALKPSARGELEITDVNLEYLSRGQLQVIPLEKN
                     FTWLDAGTADSLLAAAQTIKEIQDTTGRYVGCLEELGFYEGWISEEQVHAIGDELEMT
                     LYGKYLKCL"
     gene            complement(1730..1942)
                     /locus_tag="ACZPAQ_07570"
     CDS             complement(1730..1942)
                     /locus_tag="ACZPAQ_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903885.1"
                     /translation="MDLTENRRAVDAFTYGAGTSGYPPQELLAALLTLDGASLTSGEV
                     EELRRTCLAQGGMQESPGGTSPAGAR"
BASE COUNT          409 a          664 c          639 g          386 t
ORIGIN      
        1 ggccttcttt gaggaccgtg ccacagggtt caagaagaaa aaggaggctg cccctgtgaa
       61 acctgctgcg cccgccgccc ctgctgcggc tcccgctccc cgcgcgccct ttgccggccc
      121 cgtctactat gccgcggtgc ccgtgcccca gactcccggc tatgtgagcc gtgccatcca
      181 gccctttgcc gcgtcctacc agccctctta tctgaagatg ggagaccggg aggactgcac
      241 cggaaacttc accatcacca tcgatggcaa gccctatcag gtgtcggtgg ccaaggctga
      301 tacccccacc gccgctcctg tggccgccgc ccccgtggct gccgccgctc ctgcggccgc
      361 cgctcccgtg gcagcgccca ccccggcacc ggccgctgct cctgccgctg cccctgctcc
      421 caccgctgtt gccgccggcg agacgccggt aaacagtccc atgcccggca atatttttaa
      481 ggtggagtgc aagcccggcc aggcagtgaa ggcgggcgat gtactggtgg tgctggaggc
      541 catgaagatg gagatcgagg tctctgcccc ggtggacggc accgtcaagt ctgtatccgc
      601 cacagtgggc accgccgtca ataccgatga cctgctggta gtcctgggct gacaacagaa
      661 attcagagga taagcaacga ctccccgcca gccagttccg gctggcgggg agcctttctg
      721 accgcccggg cagggggaag agaagcactg cggccctctc acacactttt aaggaagtct
      781 taatggaaat caccttgaaa aggcagaggg aacatggtaa actgaatcat ctgaacaggc
      841 cggatggaag aggaggagtg accgcatgaa agggatcgta ttggccgcag ggaaaggcac
      901 ccgtctgtat cccatgacca agccggtttg caagccgctg cttcccgtgt acgacaagcc
      961 cctgatctac taccccatcg cgattctgat gcaggctggg atctcagaga tcctggtgat
     1021 cgtcccccct aacgagaccg ccaccttcac cgctctgctg ggggacggga gccagtacgg
     1081 tctgaagatc tcctacgcgg agcagccggt gccccggggg atcgcggacg ccctgctcat
     1141 tgggcaggag ttcgtggggg acgaccgggt ctgtctggtg ctgggggaca acattttcta
     1201 cgcccccacc ttgggagcca ccctgaaaaa ggctgcggcc agtcccgcgg ccgccacagt
     1261 gttcggctac tacgtggagg acccccgccc ctttggcgtg gtggagttcg acaagaatgg
     1321 aaaggccatt tccattgaag agaagccccg ctaccccaag tccaactata tcgtgcccgg
     1381 tctctacttc tatgatcagc aggtgatgga gatcgcccga gccctgaagc cctccgcccg
     1441 gggagagctg gagatcaccg atgtgaatct ggagtatctc agccgagggc agctccaggt
     1501 gataccgctg gaaaaaaatt ttacctggct ggacgccggc acggcggaca gtctgctggc
     1561 ggcggcccag acaatcaagg agatccagga caccaccggc cgctatgtag gctgcctgga
     1621 ggagctgggt ttctatgagg gctggatctc ggaggagcag gtccacgcca tcggggacga
     1681 gctggagatg accctctacg gcaagtatct gaaatgcctg tgaacgcctc tatcgggccc
     1741 cggcgggaga tgtcccgccg gggctttcct gcatgccgcc ctgggccaga caggtgcggc
     1801 gcagctcctc cacctctccg gacgtgaggg aggcgccatc cagcgtcagc agggcggcga
     1861 ggagctcctg aggtggatac cccgatgtac ccgctccgta tgtgaaggca tccactgccc
     1921 gcctgttctc tgtgagatcc atgtctaccc ctccttcctt ttccaaatgg cggggctgcg
     1981 cttcaagtgt ccccacataa aatgataggg gattttgtcg aaacatacaa taccaacaaa
     2041 gtggattttg gagtatcata gtgaccggag agggcgggtg gctgtcctga tccgcctg
//
LOCUS       DBMDSJ010000317         2096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12100_length_2096_cov_1.221554, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000317 DBMDSJ010000000
VERSION     DBMDSJ010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12100_length_2096_cov_1.221554"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="ACZPAQ_07575"
     CDS             complement(<1..769)
                     /locus_tag="ACZPAQ_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012267.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR0903886.1"
                     /translation="MLQGKVYGVYFSPTGGTRAYVTALAGLLSCEAEEIDLTAREERA
                     RDHVFGPEDLVILGAPVYAGRLPQLEGGIFERLRGDGTPAVFNVSYGNRAFEDALLEE
                     QDICQAHGFHGIAAGAWIAPHTFSGRIGSGRPDGTDLEKVREFAAGVRALLERPDWRS
                     CRLTVPGDRPYRPARRVEVYPAGDESCTRCMACVSRCPAGAIPPESPRSTDTGRCISC
                     LACVKNCPAGARAVAGPAFPKMVEGLEGRLLSPRREPE"
     gene            complement(980..1555)
                     /locus_tag="ACZPAQ_07580"
     CDS             complement(980..1555)
                     /locus_tag="ACZPAQ_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR0903887.1"
                     /translation="MSHEPMTAGAVQKKSKIYGLSMTAVMAAVTCVLAPMSIPIGPVP
                     ISFTNLAIYLSLYLLGWKRGTVSYLVYVLIGMVGVPVFSGFTGGMGRLLGPTGGYIVG
                     FIPMALIAGAVIDRFRNRGIQLAGMIAGTAVCYAFGTAWYCFQAGSSVGAALGLCVFP
                     FIPGDLVKMLIAMALGPMIRSRLEKAGLYRG"
     gene            complement(1610..>2096)
                     /locus_tag="ACZPAQ_07585"
     CDS             complement(1610..>2096)
                     /locus_tag="ACZPAQ_07585"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR0903888.1"
                     /translation="EHCHANLETSRRYFPQMCTTHTYEDKVTNIQRARAAGLEVCSGG
                     ILGMGESWEDRLDLAVSLAELEISSIPLNLLRPIPGTPYEGMEPISNEDVLRTVAFFR
                     YINPTAWIRMAAGRGQFLDGGAELFRSGANAAITGDMLTTTGTSMAGDRAMLLGLGYQ
                     V"
BASE COUNT          343 a          756 c          635 g          362 t
ORIGIN      
        1 gctccggttc ccggcggggg gacagcagcc gcccctccag cccctccacc atcttgggga
       61 aagccggacc ggccaccgcc cgcgcccccg ccgggcagtt cttcacacag gccagacagg
      121 agatgcagcg gcccgtgtca gtgctccggg gggattccgg agggatggct cccgccgggc
      181 accgggacac acacgccatg catcgggtgc agctctcgtc ccccgcgggg tacacctcca
      241 cccgcctggc cggccggtag ggccggtccc ccggcaccgt gagccggcag ctcctccagt
      301 ccggtcgctc cagcagcgcc cggacccccg cggcaaattc ccgcaccttt tccaggtccg
      361 tcccatccgg ccgtccggat ccgatgcggc cggagaaggt gtgaggggcg atccaggccc
      421 ccgccgcgat cccgtggaat ccatgggcct gacagatgtc ctgctcctcc agcagggcgt
      481 cctcaaaagc tctgttgccg tagctcacat taaagaccgc cggggtcccg tccccccgca
      541 ggcgttcaaa gatcccgccc tccagctggg gcagccgccc cgcatagacc ggcgccccca
      601 ggatcaccag atcctccggg ccaaagacat ggtcccgcgc ccgctcctcc cgcgccgtca
      661 gatcgatctc ctccgcctcg caggagagca acccggccag cgccgtaaca taagctctcg
      721 tcccccctgt tgggctgaaa tacaccccat atacctttcc ctgcagcata ggtctgcccg
      781 cctttctctc caaaatttgt tttcctgctg acactgtacc tctctttgtc tggcctgtca
      841 acctccggca cagggaggcg gcggccgctc tcttagaaga gagcggccgc cgctgtctgc
      901 cctgtccgga tgatttagtt ttatgcgggg cctgccgccg tcctctgggc ctccccgccg
      961 gacggctgag agaagctggt catccccgat acagtcccgc tttctccagc cgggagcgga
     1021 tcatggggcc cagcgccatg gcgatcagca ttttgaccag gtcgccgggg atgaagggga
     1081 acacgcacag ccccagggca gcccccacgg aggaaccggc ctggaagcag taccaggcgg
     1141 tgccgaaggc atagcacact gcagtgcccg cgatcatgcc ggccagctgg atgccccggt
     1201 tccggaagcg gtcaatgacc gcgccggcga tcagtgccat ggggatgaag cccacgatat
     1261 agcccccggt ggggcccagc agcctgccca tgccgccggt gaagccggag aacaccggca
     1321 cgcccaccat gccgatcagc acatagacca gatagctcac tgtcccccgc ttccacccca
     1381 gcagatagag agacaggtag atggccagat ttgtgaagga gatgggcacc ggtccgatgg
     1441 ggatggacat gggcgccagg acacaggtca ccgccgccat caccgcggtc atggacaggc
     1501 cgtagatctt gctctttttc tggaccgccc cggcggtcat gggttcgtgg ctcatagttc
     1561 tgatttcctt tccttatcgt ttggggcgtt tctcctgccc cgctgtcgct catacctggt
     1621 agcccagtcc cagcagcatg gcccggtccc ccgccatgct ggtcccggtg gtggtgagca
     1681 tgtccccggt gatggccgcg ttggccccag agcggaacag ctccgctccg ccgtccagga
     1741 actgcccccg gcccgccgcc atcctgatcc atgcggtggg gttgatgtac cggaaaaaag
     1801 ccacggtgcg cagcacgtcc tcgttggaaa tgggctccat cccctcatag ggcgtgcccg
     1861 ggatgggccg caggaggttc agcgggatgg agctgatctc cagctctgcc aggctgaccg
     1921 ccaggtccag gcggtcctcc cagctctctc ccatgcccag tatgccgccg gagcacacct
     1981 ccagccccgc cgcccgggcc cgttggatgt tggtcacctt gtcctcatag gtgtgtgtgg
     2041 tgcacatctg tgggaagtat cgccgggagg tctccagatt ggcgtggcag tgctcc
//
LOCUS       DBMDSJ010000318         2083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12183_length_2083_cov_1.527245, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000318 DBMDSJ010000000
VERSION     DBMDSJ010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12183_length_2083_cov_1.527245"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="ACZPAQ_07590"
     CDS             <1..786
                     /locus_tag="ACZPAQ_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR0903889.1"
                     /translation="RQYVVDAFADRIFEGNPAAVCVMERWPEEDLMLKITRENNLSET
                     AFVVKEGEEYRLRWFTPGGEIDLCGHATLAAAHIITRFVEPGVERVGFQTLSGLLTVR
                     AEGELLSMDFPAYQLKRLEVTEEITQALGARPSEVWRARDLLCVFDRAEDVLALKPDM
                     DKLLKVKGALMQVTAPGMEGYDCVSRSFAPKMGVPEDPVCGSGHCHIIPYWAQKLGKT
                     RFVARQASPRGGTLYCRMEGDRVILAGRAVLYARSELMLEDRP"
     gene            783..1268
                     /locus_tag="ACZPAQ_07595"
     CDS             783..1268
                     /locus_tag="ACZPAQ_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024426.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HIR0903890.1"
                     /translation="MKQRIVPLLAAALALTVMLAGCSSQEEAEPLPQGMDTEAVLTAG
                     EAVLDQLLEGDYEAVYESFREDIRADLTAQSVQDLVEPVFQEAGDYQELKSAQVTGST
                     EGEEHGIAQLLCIFSQEKVSIRVAFDPDMELIGLSAGLQKSSWSLSNLVNNFTGLFRG
                     D"
     gene            1328..>2083
                     /locus_tag="ACZPAQ_07600"
     CDS             1328..>2083
                     /locus_tag="ACZPAQ_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR0903891.1"
                     /translation="MREPKPFGLNELREMFLSFFESKGHLRLPSFSLIPQNDASLLLI
                     NSGMAPMKPWFTGEQEPPRHRVCTCQKCIRTGDIENIGKTARHGTYFEMLGNFSFGDY
                     FKREAIAWSWEFLTSPEWVGLDPDRLYPSVFEGNETTPADDEAMAIWRDEIGIPEDRI
                     FKFGKADNFWEHGSGPCGPCSEIYYDRGPAYGCGKPGCTVGCDCDRYIEVWNIVFSQF
                     DNDGEGHYTELKQKNIDTGMGLERLACVCQGVDS"
BASE COUNT          384 a          633 c          714 g          352 t
ORIGIN      
        1 agacaatatg tggtggacgc cttcgccgac aggatatttg agggcaaccc ggccgcggtc
       61 tgcgtgatgg agcggtggcc ggaggaggat ctgatgctga agatcacccg ggagaacaac
      121 ctgtctgaga cggcctttgt tgtgaaagag ggggaggagt accggctgcg ctggttcacc
      181 cccggcgggg agatcgacct atgcggccac gccaccctgg ctgcggccca tatcatcacc
      241 agattcgtgg agccgggagt ggagagggtc ggcttccaga ccctcagcgg actgctgacc
      301 gtgcgggcgg agggagagct gctgagcatg gatttccccg cctaccagct gaagcggctg
      361 gaggtgacgg aggagatcac ccaggccctg ggggcgcggc cgtcggaggt ctggcgggcc
      421 cgggacctgc tgtgcgtctt tgaccgggcg gaggacgtgc tggcgctgaa gccggacatg
      481 gacaagctgc tgaaggtgaa gggggccctg atgcaggtca cggcgccggg gatggagggg
      541 tacgactgcg tctcccgctc gtttgcccca aagatggggg tgccggagga ccctgtgtgc
      601 ggctccggac actgccatat catcccctac tgggcgcaaa agctgggcaa gacccggttc
      661 gtggcccggc aggcatcccc cagagggggg accctgtact gccggatgga aggggaccgg
      721 gtcattctgg cgggccgggc ggtgctctat gcccggtctg agctgatgct ggaggacaga
      781 ccatgaaaca gaggatcgtg ccgctgctgg cggcggccct ggccctgacc gtgatgctgg
      841 ccggctgctc ctcccaggag gaggcggagc ccctgcccca gggcatggac acagaggccg
      901 tcctgaccgc cggggaggct gtcctggacc agctcctgga gggcgactac gaggcggtat
      961 atgagtcctt ccgggaggat atccgggcag acctgaccgc ccagagcgtc caggacctgg
     1021 tggagccggt gttccaggag gcgggggact atcaggagct caagtccgcc caggtcactg
     1081 gcagcaccga gggggaggag cacggcatcg cccagctcct gtgcatcttc tcccaggaga
     1141 aggtgtccat ccgggtggcc tttgacccgg acatggagct gatcggcctg tccgccggac
     1201 ttcagaagtc cagctggagc ctgtccaatc tggtgaacaa tttcaccggc ctgttccggg
     1261 gcgactgatg atatggcccg gttatttgta acacaaaaac atgaaaaaat attggaggta
     1321 gaccaccatg cgcgagccca agccctttgg cctgaatgaa ctgagggaga tgttcctgtc
     1381 tttttttgag agcaagggac atctccgcct gcccagcttc tccctgatcc cccagaacga
     1441 cgcctccctg ctgctcatca acagcggcat ggcgcccatg aagccctggt tcaccgggga
     1501 gcaggagccc ccccgccacc gcgtctgcac ctgtcagaag tgcatccgca ccggcgacat
     1561 cgagaacatc ggcaagaccg cccgccacgg cacctatttc gagatgctgg gcaacttctc
     1621 cttcggcgac tacttcaagc gcgaggccat cgcctggtcc tgggagttcc tgaccagccc
     1681 ggagtgggtg ggcctggacc cggaccggct gtatccctcc gtctttgagg gcaacgagac
     1741 cacccccgcc gacgacgagg ccatggccat ctggcgggac gagatcggca ttccggagga
     1801 ccgcatcttc aagttcggca aggcggacaa cttctgggag cacggctccg gcccctgcgg
     1861 cccctgctcc gagatctact acgaccgggg cccggcctat ggctgcggca agccggggtg
     1921 cactgtgggc tgcgactgcg accgctatat cgaggtgtgg aacatcgtct tctcccagtt
     1981 cgacaacgac ggggagggcc actacactga gctgaagcag aagaacatcg acaccggcat
     2041 gggactggag cgcctggcct gcgtgtgcca gggggtggac tcc
//
LOCUS       DBMDSJ010000319         2068 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12273_length_2068_cov_1.749873, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000319 DBMDSJ010000000
VERSION     DBMDSJ010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12273_length_2068_cov_1.749873"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="ACZPAQ_07605"
     CDS             <1..415
                     /locus_tag="ACZPAQ_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR0903892.1"
                     /translation="CALGMLFIQSDYAALVGVIVGTTALIPVMGAYIGAVLPAFLLLM
                     VDPWRALAFLVFLGILQQIEGNVIYPRVVGTSIGLPGIWVLAAVTVGGGLFGLLGVLL
                     SVPAASVLYTLLKRDVHRRLEEKMPAPEQAEEGTE"
     gene            681..2066
                     /locus_tag="ACZPAQ_07610"
     CDS             681..2066
                     /locus_tag="ACZPAQ_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903893.1"
                     /translation="MRRHIGLALLTAALSVLLTGCLFRSTEDLYALPERFPGYEDLTG
                     KRSEIQAGLEMEYGTTVETAVIYSGDNTSVIQFQDMDGDGSQETAVISFRIPGAERSL
                     RVYFLTTQGDTERYEISAVVEGDGTGINAIDYVELSGSGKKEVVVSWRTIEGVNQLGV
                     YSLDELESNETVPVASQLLMTGYSGYSLLDIDRDTRTELAVIRLDPAGVDSTVEVYGW
                     QNGMLDRLGTARLSAGITALASNGVRINYLTGVIPALYITSTLTDGGQATDIVAMRDG
                     SLVNLTMNQETGVSVETIRGYGDIRPMDVNSDTILEMPRPHLLPTYGENLSSDFWLID
                     WSQYDVGGRAAPVFTTYHNMSDQWYLIIPEEWVDQITISRSDSSGIRAVIFSLWNGQK
                     KAPTPFLAIYKLTGINSSSQAAKEGRFILAEDGDAIYAAEFFESGWNCGLDEAGVRDS
                     FRLILSNWAGD"
BASE COUNT          389 a          611 c          694 g          374 t
ORIGIN      
        1 gtgcgccctg gggatgctgt tcatccagtc cgactacgcc gccttggtgg gggtgatcgt
       61 gggcaccacg gccctgatcc cggtgatggg ggcttatatc ggggcggttc tgcccgcctt
      121 tctgctgctg atggtggacc catggcgtgc attggcgttt ctggtattcc tgggcatcct
      181 ccagcagatc gagggcaatg tgatctatcc cagagtggtg ggcacctcca tcggcctgcc
      241 cggtatctgg gtgctggctg cggtgacggt gggcggcggc ctgttcggcc tgctgggcgt
      301 gctgctcagc gtgccggcgg cgtcggtgct gtacaccctg ttgaagcggg atgtgcaccg
      361 ccggctggag gagaaaatgc ccgccccgga gcaggcagaa gagggaacag aataggcaca
      421 ggggccggcg ggatctccgc cggccccttc tgacttgcgg ctgcccggcc tccggggtgg
      481 tcctcccctc caggcccggt gccgggatgc ggtccgatgg gacatgggtc cccctgagcg
      541 agaaatcgag ccacagggac gcaggccgaa aagttctttc agattttgaa aaaagaatac
      601 ccaagcgcaa agatttatgg tatgatgaca aaagggtgat tttccagctg gcagagccgg
      661 ccgagaggag ggaacggcac atgagaagac atatcgggct ggcgctgctg acggcggcgc
      721 tgtctgtcct gctgaccggc tgcctgttcc gctccacaga ggatctgtac gcccttccgg
      781 agcgctttcc aggctatgag gacctgaccg gcaagcgcag tgagatccag gccggcctgg
      841 agatggagta tgggaccacg gtggagactg cggtcatcta ctccggcgac aacacctctg
      901 tgatccagtt tcaggatatg gacggcgacg gctcccagga gactgcggtc atctccttcc
      961 gcatcccggg ggcggagcgc tccctgcggg tctatttcct caccacccag ggagacaccg
     1021 agcgctatga gatcagcgcg gtggtggagg gggacggcac cggaatcaac gccatcgatt
     1081 atgtggagct cagcggcagc ggcaaaaagg aagtggtggt cagctggcgc accatcgagg
     1141 gcgtcaacca gctgggcgtc tactccctgg acgagctgga gagcaatgag accgttccgg
     1201 tggcctccca gctcctgatg accggataca gcggatacag cctgctggac atcgaccggg
     1261 atacccggac ggagctggcg gtgatccgcc tggacccggc gggcgtggac agcacggtgg
     1321 aggtctacgg ctggcagaat gggatgctgg atcggttggg gaccgcccgg ctctccgccg
     1381 gcatcacggc cctggcctcc aacggcgtgc ggatcaacta cctcaccggg gtgatccccg
     1441 ccctctatat caccagcacc ctgacggacg gaggacaggc cactgacatc gtggccatgc
     1501 gggacggctc gctggtcaac ctgaccatga accaggagac aggcgtgagc gtggagacca
     1561 tccggggcta tggtgatatc cgccctatgg atgtgaacag cgacaccatc ctggagatgc
     1621 cccgccccca cctgctgccc acctacgggg agaacttgtc cagcgacttc tggctcatcg
     1681 actggagcca gtacgacgtg ggggggaggg cggccccagt ctttaccact tatcacaata
     1741 tgtccgatca gtggtatctg atcatcccag aggagtgggt ggaccagatc accatctccc
     1801 gaagtgacag cagcggcata cgtgcggtca tcttctccct ctggaacggg cagaagaagg
     1861 ctcccacccc gtttttggcc atctacaagc tgaccggcat caacagcagc agtcaggccg
     1921 ccaaggaggg gcgctttatt ctggcggagg acggcgacgc catctacgcg gcagaatttt
     1981 ttgagagcgg ctggaactgc ggattggatg aggccggcgt gcgggacagc ttccgcctga
     2041 tcctgagcaa ctgggccgga gactgacc
//
LOCUS       DBMDSJ010000320         2065 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12290_length_2065_cov_1.781568, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000320 DBMDSJ010000000
VERSION     DBMDSJ010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12290_length_2065_cov_1.781568"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="ACZPAQ_07615"
     CDS             <1..646
                     /locus_tag="ACZPAQ_07615"
                     /inference="COORDINATES: protein motif:HMM:NF024136.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HIR0903894.1"
                     /translation="GLSTQMVRDSEGHFAEDLDDCFRRGVSVYEELEQKLPCLAMPSV
                     PCKPMFFSRLQSMMGFTGVYFPFTGEANVNVDAPACLVPATIAHEMSHQRMVFSELEA
                     NFVGIAAAVSCGDPVFQYSGWLMGLIQLCNALYAVSPDLWYQIAAASFTPELSTDWED
                     NNAYWRALESPVEEAAAQTFDTFLKSNGQDLGIQSYGACVDLLVTWFGDEAGAF"
     gene            781..1725
                     /locus_tag="ACZPAQ_07620"
     CDS             781..1725
                     /locus_tag="ACZPAQ_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR0903895.1"
                     /translation="MKRPILSMLLSVLLLLPGCTQAPSGSSQEEGLTVLATTYPVYLF
                     ARTVAQGVEGVTVERLNTGSVSCLHDYTLSVDDMKKIEGADVIAMNGAGLEDFMDDAL
                     AASSAAVIDCSQGVALLENADHIHEEGDGGHDHGHWDPHYWMDPGNAAVMVRNLQEGF
                     ALADPDHGDAYARNGEEAAGQLPSFAGDCTQALEGAEGAALITFHDGFRYFAQALGLP
                     LLASIEEEEGSEASAMEINEITQLVKEHQLPVIFTEVNGSDATANAISRETGCQVAQL
                     TMLMDGPEEGDLSTYLEGVRSNVNTIVSFFGGEELDQL"
     gene            1722..>2065
                     /locus_tag="ACZPAQ_07625"
     CDS             1722..>2065
                     /locus_tag="ACZPAQ_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0903896.1"
                     /translation="MRKIKHGRLAGGCSDACCLKLEGLSVHIQGEDILEDVSFHLHCG
                     EIAALIGPNGAGKSSLFRSILGQMPYSGTITFSPAGGPAVRLSDGANAPLAARGTRPL
                     IGYVPQSPSFDRG"
BASE COUNT          353 a          706 c          631 g          375 t
ORIGIN      
        1 aggcctctcc acccagatgg tccgagacag cgagggccac ttcgctgagg atctggacga
       61 ctgtttccgc cggggagtct ccgtctatga ggagctggag cagaagctcc cctgtctggc
      121 tatgccctcc gtgccctgca agcccatgtt cttctcccgg ctgcagagca tgatgggctt
      181 caccggcgtc tacttccctt tcaccggcga ggccaacgtc aacgtggacg ctcccgcctg
      241 cctggtgccg gccaccatcg cccacgagat gtcccaccag cggatggtct tctccgagct
      301 ggaggccaat tttgtgggca tcgccgcggc ggtgtcctgc ggcgatccgg tgttccagta
      361 ctccggatgg ctgatgggcc tgatccagct gtgcaacgcc ctctacgccg tctccccgga
      421 tctgtggtat cagatcgcgg cggcctcctt tacaccggag ctgtccaccg attgggagga
      481 caacaacgcc tattggcgtg ctctggagtc ccccgtggag gaggccgctg cccagacctt
      541 tgacaccttt ctgaagagca acggccaaga tctgggcatc cagtcctacg gtgcctgcgt
      601 ggacctgctg gtgacctggt tcggcgacga agccggcgcc ttctgagccc tgacgggcgg
      661 agtgttcttc tcccttctgc cggggcaaaa agagactttc cccgctatcc gtttacaaat
      721 ccccgccatc gcgctataat aatatgattc gatccactct gatgaaattt gaggtatccc
      781 atgaaacgac ccattttatc catgctcctg tccgtcctgc tcctgcttcc cggctgcacc
      841 caggccccct cgggctcctc ccaggaggag gggctgaccg tcctggccac cacctatccg
      901 gtctatctct tcgcccggac cgttgcccag ggcgtggagg gcgtcaccgt ggagcggctg
      961 aacaccggct ctgtgagctg cctccacgac tataccctgt cggtggacga catgaaaaag
     1021 atcgagggtg cggatgtcat cgccatgaac ggtgccgggc tggaggactt tatggatgac
     1081 gccctggccg cctccagcgc ggcggtcatc gactgctctc agggggtggc gctgcttgaa
     1141 aacgcggacc acatccacga ggagggagac ggtggccacg accacggcca ctgggacccc
     1201 cactactgga tggacccagg aaacgccgcc gtcatggtgc gcaatctcca ggagggcttc
     1261 gccctggcgg acccggacca cggagacgcc tacgcccgca atggggagga ggccgccggc
     1321 cagctgccca gctttgccgg cgactgcacc caggccctgg agggggcaga aggcgcggcc
     1381 ctcatcacct tccacgacgg cttccgctac tttgcccagg cactgggcct gcccctgctg
     1441 gcctccatcg aggaggagga gggcagcgag gccagcgcca tggagatcaa tgagatcacc
     1501 cagctggtga aggagcacca gctgcccgtc atcttcaccg aggtcaacgg ctccgacgcc
     1561 accgccaacg ccatctcccg ggaaaccggc tgtcaggtgg cccagctcac catgctcatg
     1621 gacggcccgg aggaggggga cctgtcaacc tatctggaag gcgtgcggag caacgtgaac
     1681 accatcgtat ccttcttcgg gggagaggag ttggatcagc tgtgagaaag atcaagcacg
     1741 gcaggctggc cgggggctgc tccgacgcct gctgcctgaa gctggagggc ctgtcggtcc
     1801 acatccaagg ggaggacatc ctggaggatg tgtccttcca cctccactgc ggggagatcg
     1861 ccgccctcat cggccccaac ggggcgggca agtcctccct gttccgcagc atcctggggc
     1921 agatgccata ctccggcacc atcaccttct cccctgccgg cgggcctgcc gtccggctgt
     1981 ccgacggggc caacgctccc ctcgccgccc gggggacccg ccccctcatc ggctatgtgc
     2041 cccagtcccc cagctttgac cgggg
//
LOCUS       DBMDSJ010000321         2063 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12304_length_2063_cov_1.781346, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000321 DBMDSJ010000000
VERSION     DBMDSJ010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12304_length_2063_cov_1.781346"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(176..2023)
                     /locus_tag="ACZPAQ_07630"
     CDS             complement(176..2023)
                     /locus_tag="ACZPAQ_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR0903897.1"
                     /translation="MTKKQKKMLSRILISAVLFLLLYTVPAQGVVRLLLYLIPYAVIG
                     WDVLWRAVKNIRNGQVFDENFLMSIATIGAFGCGEYPEAVAVMLFYQVGELFQSVAVG
                     RSRQSIAALMDIRPDYANMERDGQLVQVDPDEVAVGDTIVIKAGERVPLDGIVTQGNS
                     ALDTAALTGESLPRDVAAGDEVISGCVNLSGLLHVRVTRPFGESTVAKILDLVENSSE
                     KKAKAEHFITKFARYYTPAVVFAALALAVIPSLLLEGGWGVWVPRALNFLVVSCPCAL
                     VISIPLSFFGGIGGASKHGILVKGSNYLEALARAGIVVFDKTGTLTRGTFEVTVIHPQ
                     QLSEQELLELAALAEHFSTHPISLSIQAACRPAPDPARVTDVKEIAGRGVQAVVDGRT
                     VLAGNQKLMDQFQISFEDRCRHMGTIIHVAVDGEYAGHIVISDQPKPEAAQALSDLKA
                     AGVRRTVMLTGDAQSAAEGVARELGLDEFHAELLPGDKVDQVERLLAQKQPKENLVFV
                     GDGINDAPVLSRADIGVAMGALGSDAAIEAADIVLMDDDLSKLAQAVRIARKTLSIVR
                     QNIVFALAVKGLVLVLSALGYVGMWWAVFADVGVSVLAILNASRMLRAK"
BASE COUNT          388 a          639 c          680 g          356 t
ORIGIN      
        1 aatctgttcg tcagcgactt gatcgccctg aggaccgtgc tgaacgccca gtacgacgat
       61 tttttccggg ggctggtgcc catcgaccgg tacaaggatg agacgcagac gtgagaaaac
      121 caggtgccgc cccggggggc ggcacctggt tttttagtct taaaagggac ctttgtcatt
      181 ttgcccgcag catccggctg gcgttgagga tggccaggac cgagacgccc acgtcggcga
      241 agacggccca ccacatgccc acatagccca gagcggacag gaccagcacc agccccttca
      301 ccgccagggc gaagacgatg ttctgacgga cgatggacag ggtcttgcgg gcgatgcgca
      361 ccgcctgggc cagcttggag aggtcatcgt ccataagcac gatgtccgcc gcctcgatgg
      421 ccgcatcaga gcccagcgct cccatagcca caccgatgtc cgcccgggag aggacggggg
      481 catcgttgat gccgtccccc acaaaaacaa gattttcctt gggctgcttc tgggccagca
      541 ggcgctccac ctggtccact ttgtctccgg gcagcagctc ggcgtggaac tcatccagac
      601 ccagctcccg ggccacgccc tcggcggcgc tctgggcgtc gccggtgagc atgaccgtgc
      661 ggcggactcc ggcggccttc aggtcagaaa gggcctgggc ggcctccggc ttgggttggt
      721 cggagatgac gatgtgaccg gcgtattccc catccaccgc cacgtggatg atggtgccca
      781 tgtggcggca gcggtcctca aaggagatct ggaactggtc catcagtttc tgattgccgg
      841 ccagcacggt cctgccatcc accacggcct ggacgccccg gccggcgatc tccttgacgt
      901 cggtgacccg ggccgggtct ggggcgggcc ggcaggcggc ctggatggac agagagatgg
      961 gatgggtgct gaaatgctcc gccagggcgg ccagctccaa cagttcctgt tcgctgagct
     1021 gctgggggtg gatcaccgtg acctcgaagg tgccccgggt gagggtgccg gtcttgtcaa
     1081 agaccacgat gcctgcccga gccagggcct ccaggtagtt gctgcccttc accagaatgc
     1141 catgcttgga ggcaccgccg atgccgccga agaagctgag ggggatggag atcaccaagg
     1201 cgcaggggca ggacaccacc aggaagttca gcgctctggg gacccacacg ccccagcctc
     1261 cctcaagcag cagggagggg ataacggcca gggccagggc ggcaaaaacc acggcggggg
     1321 tgtagtaccg ggcgaattta gtgataaagt gttccgcctt ggccttcttt tcgctggaat
     1381 tctccaccaa gtccaggatc ttggccacgg tggattcccc aaagggcctg gtgacccgga
     1441 catggagcag gccggacagg ttgacacagc cgctgatcac ctcgtccccg gcggctacgt
     1501 cccggggaag ggattccccg gtgagggcgg cggtatccag cgcgctgttt ccctgggtga
     1561 ctatgccatc cagggggacc cgctcgccgg ccttgatgac gatggtgtct cccaccgcca
     1621 cctcatccgg gtccacctgg accagctggc cgtcccgttc catattggca tagtcaggac
     1681 ggatgtccat cagggcggcg atggactgcc ggctgcgccc cacagccacg ctctggaaga
     1741 gctcgcccac ctgatagaag agcatgacgg ccacggcctc ggggtactcc ccgcagccga
     1801 aagcgccgat ggtggcgatg gacatgagaa agttttcgtc aaagacctgg ccgttgcgga
     1861 tgttcttcac cgccctccac agtacatccc agccgataac agcatagggg atgaggtaga
     1921 gcaggagacg gaccaccccc tgggcgggga cggtgtagag gagcaaaaag aggacggcgc
     1981 tgattaggat gcgggagagc atttttttct gcttttttgt catggcggtt ccctccagag
     2041 gcggggacag aggggaagct ccc
//
LOCUS       DBMDSJ010000322         2062 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12310_length_2062_cov_2.047425, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000322 DBMDSJ010000000
VERSION     DBMDSJ010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12310_length_2062_cov_2.047425"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="ACZPAQ_07635"
     CDS             <1..262
                     /locus_tag="ACZPAQ_07635"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529858.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR0903898.1"
                     /translation="VRLYDNLFTDPDPDAGDKNFLDCLNPNSLEVLEGCKLEASLADA
                     QAPAHYQFLRLGYFCVDSKDSKPGALVFNRAVSLKDSFKPGK"
     gene            complement(343..1365)
                     /locus_tag="ACZPAQ_07640"
     CDS             complement(343..1365)
                     /locus_tag="ACZPAQ_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903899.1"
                     /translation="MERMEEYQRMREEEAGRPVPAELACLETRVRARAAERPRRRRGP
                     VLAAVCLCLVLGTGMAWGMGGFAALDRLLGENAGQLIPFYQEVGETVRLEDGMVEIQG
                     VTFTSHSCTVVYRLTAPNGEEAAETPPFYWKLIQGEAGPKESPFSSFVEPSRNGQDLG
                     GNLGWEEVLEGEKRGSTYQVFTVSVEEDMSQVEDPKLLLYFSRAVDGVPSADDVPGGT
                     LLTIPAEEVLEERVVRVPGDGEIRQVRVSPALISAQTAVPAEGNPVNLEVLREAPEIR
                     LLYRDGTAWELEQEHVFQKEGRPDYCANAGEDGLLILNWFLREAPPDLEDLEAVEVNG
                     IRCPVE"
     gene            complement(1368..1832)
                     /locus_tag="ACZPAQ_07645"
     CDS             complement(1368..1832)
                     /locus_tag="ACZPAQ_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR0903900.1"
                     /translation="MNDQEYWQGVEQCRERLWRIARMWLSDDSAAMDVVDEAVFRGWR
                     ACGRLRHPEYFTTWLTRILLNECSREKKRQDREQAVDQLPEQAWEDPDHLPLRAAVQA
                     LPEQLRAVILLRYFFDCTLRETAELLRMPRSTVHTREKKALKLLKLELSEEV"
BASE COUNT          334 a          765 c          559 g          404 t
ORIGIN      
        1 ggtacgcctg tacgacaacc tgttcaccga ccccgacccg gacgccgggg ataagaactt
       61 cctggactgc ctgaacccca actccctgga ggtgctggag ggctgcaaac tggaggcctc
      121 tctggccgat gcccaggccc ccgcccacta ccagttcctc cggctgggtt acttctgcgt
      181 ggacagcaaa gattccaagc ccggcgctct ggtgttcaac cgggcggtct ccctgaagga
      241 cagcttcaag cctggcaagt gagccgggcg cctgtccacc ctgaaacgat aaaacaccgc
      301 gccgcgtaca gctttcctgt acgcggcgcg gcatttctgc gcctactcga cgggacaccg
      361 gatcccgttc acctccaccg cctccagatc ctccagatcc gggggggctt cccgcaggaa
      421 ccagttcagg atcagcagac cgtcctctcc cgcgttggca cagtagtcgg gccgtccttc
      481 tttctgaaag acatgctcct gctccagctc ccaggccgtc ccatcccggt agagcagccg
      541 gatctccggg gcctcccgga gcacttcgag gttgacggga tttccctcgg caggcacagc
      601 cgtctgggcg gagatcagcg ccgggctgac tctcacctgc cggatctccc cgtcccccgg
      661 tacccggacc acccgctcct ccagcacctc ctccgccggg atagtcagca gagtgccgcc
      721 gggcacgtca tcggcagagg ggaccccgtc caccgccctg gaaaagtaga gaaggagctt
      781 ggggtcttcc acctgactca tgtcctcctc cacgctgacg gtaaagacct gataggtgct
      841 cccccgcttc tctccctcca gcacttcctc ccagccgagg tttcctccca gatcctgccc
      901 attccggctg ggctccacaa aggaggagaa gggggactcc ttcgggcccg cctctccctg
      961 gatcagcttc cagtagaagg gaggcgtttc cgccgcctct tctccgtttg gggcggtcag
     1021 ccggtagacc acggtacagc tgtggctggt aaaggtgacg ccctggatct ccaccatccc
     1081 gtcctccagg cggaccgtct ctcccacctc ctgatagaag gggatcaact gtcccgcgtt
     1141 ttcccccagc agccggtcca gcgcggcgaa accgcccatg ccccaggcca tgccggtccc
     1201 caggaccaga cacaggcata cagccgccag gaccggtcct cttctcctcc tcggccgctc
     1261 cgccgcccgg gccctgaccc tggtctccag gcaggccagc tccgccggga cgggccggcc
     1321 ggcctcctct tcccgcattc tctggtattc ctccatgcgc tccatcttca gacctcctca
     1381 ctcaattcca gcttcaacag cttcagcgcc tttttctccc gtgtgtggac cgtgctcctc
     1441 ggcatccgca gcagctctgc cgtctcccgc aaagtacagt cgaagaaata gcgcagaagg
     1501 atcactgctc tcagctgctc cggcagtgcc tggaccgccg ccctcagagg caggtggtcc
     1561 gggtcctccc aggcctgctc cggcagctgg tccaccgcct gttcccggtc ctgccgtttt
     1621 ttctcccggc tgcactcgtt gagcaggatg cgggtgagcc aggtggtgaa atattccggg
     1681 tggcgcagtc tcccgcaggc cctccagccc cggaacaccg cctcgtccac cacgtccatg
     1741 gccgcgctgt catcggacag ccacatccgc gcgatgcgcc acagccgttc ccggcactgt
     1801 tccaccccct gccagtactc ctggtcgttc acgctgctcc cccctttctg cctattagac
     1861 cctcgggagc ccccctttgt ccaggggagt acaaaatttt ttaaaaaagt cccggcctga
     1921 cgcatttgcg ccaggccggg actttttatg cacacttttt tctgttggtt cagccaacaa
     1981 caaaggtcag ggtgtacgtc gccttggtgc tcccgtcggg tccggtgacc tcgatggtgg
     2041 tcttgccgga ggcggacacg ct
//
LOCUS       DBMDSJ010000323         2059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12330_length_2059_cov_1.960674, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000323 DBMDSJ010000000
VERSION     DBMDSJ010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12330_length_2059_cov_1.960674"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="ACZPAQ_07650"
     CDS             complement(<1..232)
                     /locus_tag="ACZPAQ_07650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0903901.1"
                     /translation="MTDIIVAGGGPAGLTAALYAVRAGRSVLVLEEAAPGGQINFSPL
                     VENYPGLPGVSGLQLSEALTRQAEEQGVELDYA"
     gene            complement(360..>2059)
                     /locus_tag="ACZPAQ_07655"
     CDS             complement(360..>2059)
                     /locus_tag="ACZPAQ_07655"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HIR0903902.1"
                     /translation="FSSFDTYLAPFVKVDHLTQKEVKQCIQSFVYGVNTPSRWGTQAP
                     FSNITLDWTVPKDLENLPAIVGGREMDFTYGDCKTEMDMVNKAFIEIMIEGDANGRGF
                     QYPIPTYSITRDFDWSETENNKLLFEMTAKYGTPYFSNYINSDMEPSDVRSMCCRLRL
                     DLRELRKKSGGFFGSGESTGSVGVVTINLPRIAYLAKDESDFYQRLDKLMDIAARSLK
                     TKRTVITKLLEAGLYPYTKRYLGTFDNHFSTIGLIGMNEVGLNAGWLRADLTHKETQQ
                     FTKDVLNHMRERLSDYQEQYGDLYNLEATPAESTTYRFAKHDKEEFPDIITANENGTP
                     YYTNSSHLPVGYTEDIFSALDVQDELQTLYTSGTVFHAFLGEKLPDWKAAAELVRKIA
                     ENYKLPYYTMSPTYSVCADHGYLSGEQHTCPICGKPAEVYSRITGYYRPVQNWNDGKT
                     QEFKDRKVYDLTASHLRKAGRAGSQVTFSQEGAPQASGGESLLFTTRTCPNCRQAEAL
                     LQKAGVPYQKVVAEESPDLTTRYGVRQAPTLVVQGESITGLGPIKRFAEEHRAREVG"
BASE COUNT          337 a          610 c          708 g          404 t
ORIGIN      
        1 gggcatagtc cagctccacc ccctgctcct ccgcctggcg ggtgagggcc tcagagagct
       61 ggaggccgct gacgccgggc aggccggggt agttctccac caggggggag aagttgatct
      121 gtccgccggg agcggcctcc tccaggacca gcacggagcg gccggcccgg acggcgtaca
      181 gggcggcagt gaggccggcg gggccgcccc ccgccacgat gatatcagtc ataagggtcc
      241 tctctccttt gaaaaagcgg ctccaggaga cccggagccg ctctcttttc ctgatgtccc
      301 gatccgtctc agtgcccccg ccgccgcaag gggggaggga acttgggatc aaggcgcggt
      361 cagcctactt cccgggcccg gtgctcctcc gcgaaccgct tgatggggcc cagtccggtg
      421 atgctctccc cctggaccac cagggtgggg gcctgacgca ccccgtaccg ggtggtcagg
      481 tcgggagact cctctgccac caccttctga tagggcactc ccgccttctg gagcagggcc
      541 tccgcctgac ggcagttggg gcaggtacgg gtggtgaaca gcagggactc cccgccggac
      601 gcctgggggg cgccctcctg ggagaaggtc acctggctgc cggcccggcc cgccttccgc
      661 aggtgggagg cggtgaggtc atataccttc cggtccttga actcctgggt cttgccgtca
      721 ttccagttct gcaccggccg gtagtaaccg gtgatgcggc tgtacacctc cgcaggcttg
      781 ccgcagatgg ggcaggtgtg ctgctccccg gacagatagc cgtggtcggc gcacacggag
      841 taggtggggg acatggtata gtagggcagc ttatagttct ctgcgatctt ccgcaccagc
      901 tccgccgccg ccttccagtc aggcagcttc tcccccagga aggcgtggaa cacagtgccg
      961 gaggtgtaga gggtctgaag ctcgtcctgc acgtccagag cggagaagat gtcctcggtg
     1021 tagcctacgg gcaggtggga ggagttggtg tagtagggag ttccgttctc gttggcggtg
     1081 atgatgtcag gaaactcctc cttgtcgtgc ttggcaaagc ggtaggtggt ggactcggcg
     1141 ggggtggcct ccaggttgta gaggtcgccg tactgctcct gatagtcgct cagccgctcc
     1201 cgcatgtgat tgagcacgtc tttggtgaac tgctgggtct ccttgtgggt caggtcggcc
     1261 cggagccacc cggcgttcag tcccacctcg ttcataccga tgaggccgat ggtggagaag
     1321 tggttgtcaa aggtccccag atagcgcttg gtataggggt agagccccgc ctccagcagc
     1381 ttggtgatga cggtgcgctt ggtcttgagg gaccgggcgg cgatgtccat cagcttgtcc
     1441 agccgctggt agaagtcgga ctcgtccttg gccagatagg cgatgcgggg caggttgatg
     1501 gtgaccaccc ccacgctccc agtggactcg ccggagccga agaagccgcc ggactttttc
     1561 cgcagctccc gcaggtccag gcgcagacgg cagcacatgg agcgcacgtc ggagggctcc
     1621 atgtcggagt tgatgtagtt ggagaagtag ggggtgccgt acttggcggt catctcaaac
     1681 aggagcttgt tgttctccgt ctcagaccag tcgaagtccc gggtgatgga gtaggtgggg
     1741 atggggtact ggaagccccg tccgttggcg tcgccctcga tcatgatctc gatgaacgcc
     1801 ttgttcacca tgtccatctc ggtcttgcag tccccgtagg tgaagtccat ctccctgccc
     1861 cccacgatgg cgggcaggtt ctccagatcc ttgggcacgg tccagtccag ggtgatgttg
     1921 gagaagggag cctgggtgcc ccagcggctg ggggtgttca ccccgtagac gaaggactgg
     1981 atgcactgct tgacctcttt ctgagtcaag tgatccacct tgacaaaggg agccagatag
     2041 gtgtcaaagg aggagaacg
//
LOCUS       DBMDSJ010000324         2059 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12333_length_2059_cov_1.527068, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000324 DBMDSJ010000000
VERSION     DBMDSJ010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12333_length_2059_cov_1.527068"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(183..578)
                     /locus_tag="ACZPAQ_07660"
     CDS             complement(183..578)
                     /locus_tag="ACZPAQ_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HIR0903903.1"
                     /translation="MIGYGGFGMANAMFTIVPIFIGVVFVLILAGIAVQAVRGAAQWK
                     RNNDAPVLTVEAEVAAKRMDVRTYRHGGTEHSRRHASSRTYYYATFQVESGDRMELEV
                     PDMEYGLLVEGDRGRLTFQGTRFQGFRRT"
     gene            complement(575..1252)
                     /gene="nth"
                     /locus_tag="ACZPAQ_07665"
     CDS             complement(575..1252)
                     /gene="nth"
                     /locus_tag="ACZPAQ_07665"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR0903904.1"
                     /translation="MKTIEEIWSIVNGLKELYPDGVCSLDYRKDYELLFSVRLAAQCT
                     DERVNKVTPALYARFPTLEALAEADIAEVERYIHSTGFFRAKAKDIVLASQMLLRDYG
                     GRVPDTMEELLKLPGVGRKTANLILGDVYHTPGVVVADTHCIRISGLLGLTDGTKDPG
                     KVEQQLRAILPPEESNDFCHRLVLHGRAVCIARRPQCQSCVLRPWCDHFAKSGQTARG
                     TEGREPA"
     assembly_gap    1551..1560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1561..>2059
                     /gene="glyA"
                     /locus_tag="ACZPAQ_07670"
     CDS             <1561..>2059
                     /gene="glyA"
                     /locus_tag="ACZPAQ_07670"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980929.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR0903905.1"
                     /translation="ADPEVGRAIQAEYDRQQQNIELIASENVVSSAVLAAAGTVLTNK
                     YAEGYPGKRYYGGCQCVDVVEDIAIQRARQLFGAGFANVQPHSGAQANYAVYQALCQY
                     GDTVLGMSLDNGGHLTHGSPVNFSGKQYKMVAYGVDPVTHRIDYDQVRDLARRHRPKM
                     IIAGAS"
BASE COUNT          383 a          719 c          554 g          393 t
ORIGIN      
        1 ccgaaattca gctcaaagat ctgggacaca cagtaggcca ctgagatgat gggagatacc
       61 cccagccccg tctttgtgtt cagggtgatc cccgccgcca ggaccaccat tcccagaaca
      121 taaaagatcc agcgattcac tttttccact ggtccgcttc tcccctcatt cggtctctcc
      181 actcatgtgc ggcggaaccc ctgaaagcgg gtgccctgga aggtcagccg cccccggtcc
      241 ccctccacca gaaggccgta ctccatgtcg ggcacttcca gctccatccg gtccccgctc
      301 tccacctgaa aagtggcgta gtaataggtc cggctggagg catgtctcct gctgtgctca
      361 gtccccccgt ggcggtaggt gcgcacgtcc atgcgcttgg ccgccacctc cgcctccact
      421 gtcagcaccg gggcgtcgtt gttccgcttc cactgggcgg ccccacggac cgcttggact
      481 gcaatgcccg ccaggatcag cacgaagaca accccgataa aaatggggac aatggtgaac
      541 atggcgttgg ccatgccgaa tccgccgtat ccgatcatgc tggctctctc ccttctgtcc
      601 cccgggctgt ctgcccgctc tttgcgaagt ggtcacacca gggccgcagg acgcagctct
      661 ggcactgggg acggcgtgcg atgcacacag cacgcccgtg gaggaccagc cggtggcaga
      721 agtcgttgga ctcctccggc ggcaggatgg cccgcagctg ctgctccacc tttcccgggt
      781 ccttggtgcc gtcggtcagg cccagaaggc cggaaatgcg gatgcagtga gtatccgcca
      841 ccaccacccc cggggtgtgg tacacgtccc ccaggatcag gttggccgtc ttccggccca
      901 ccccgggcag cttcagcagc tcctccatgg tgtccggcac ccgccctccg tagtcccgca
      961 gaagcatctg ggaggccagc acgatgtcct tcgccttggc ccggaaaaag ccggtggagt
     1021 ggatgtacct ctccacctcc gcgatgtccg cctccgccag cgcctccagg gtagggaagc
     1081 gggcatagag ggccggtgtc accttgttca cccgctcatc ggtgcactga gccgccagac
     1141 gcaccgagaa cagcagctca tagtcctttc tgtaatccag agagcagacc ccatccgggt
     1201 agagctcctt cagcccattg acaatgctcc agatctcttc tatggttttc atcctgtctc
     1261 acacctttcg gaccgctccg ccctttaggc gaggcctgtc ctgtcatatc cggggcctgc
     1321 gcccctcact cccgctccca gtcagagggg ccaaaggtca ggacggcctc tcccctgctg
     1381 gtctttacat cggggcttct tcccccatat cttacaccag caccccggac cttgtaaagc
     1441 aagctcttcc tttcttccct attttcattg taatttccgc cccatctgct ataataggga
     1501 agacggcccc ggccccgccg ggcatcaact gacaaaggag tgttcccatt nnnnnnnnnn
     1561 ccgccgaccc cgaggtcggc agggcaattc aggcggaata tgaccgccag cagcagaaca
     1621 tcgaactcat cgcctcggag aacgtggtca gctccgccgt gctggccgct gccggtacgg
     1681 tgctcaccaa caaatacgcg gagggctacc ccggaaaacg gtattacggc ggctgccagt
     1741 gcgtagatgt agtggaggac atcgccatcc agcgggccag gcagctcttc ggcgccggct
     1801 ttgccaatgt acagccccat tccggcgcac aggcaaacta cgctgtctac caagccctct
     1861 gccagtatgg cgacaccgtc ttgggcatga gcctggacaa tggcggccac ctgacccacg
     1921 gctcccccgt caacttctcc gggaagcagt acaaaatggt ggcctacggc gtagaccccg
     1981 taacccaccg catcgactat gaccaggttc gagatctggc cagacgtcac cgccccaaaa
     2041 tgatcatcgc cggtgcctc
//
LOCUS       DBMDSJ010000325         2057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12352_length_2057_cov_1.338446, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000325 DBMDSJ010000000
VERSION     DBMDSJ010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_2057_cov_1.338446"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="ACZPAQ_07675"
     CDS             complement(<1..556)
                     /locus_tag="ACZPAQ_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013793.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR0903906.1"
                     /translation="MRTFAIVNRKGGVGKTTTAVNLAYVLATSCHLRVLLVDADGQAN
                     ATQILLPRGEYDGLRALMRGMAICYDELVVHTDVEGLDVLPASEDLWALDLEAAGGEG
                     GRSYRTLRDMREAVDEDGAYDVMVIDCPPNLSAACVSAILASDAVIIPVLSDACSATG
                     VADLMEQIDSLRYIRPEIRVAGVLV"
     gene            complement(575..1894)
                     /locus_tag="ACZPAQ_07680"
     CDS             complement(575..1894)
                     /locus_tag="ACZPAQ_07680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR0903907.1"
                     /translation="MADYLLAQSSVLGSMLISPQIVGDVMTNLQPEDFTEPMGQEVFL
                     AIRALHLAGEKIDPVCVLNQMGGPDPGRRKYLLALMRETPTAQNYREYMDIVREQSRL
                     REIQRAGLALAGTDITLETAREPVSRLYELMMDRRGVECVDMEQGMLDFYAELERTPR
                     YLPWGMDFLDEGLTAEGGDFVVLGGYPSDGKTALALSMAYAQAETLRVGFFSLETKTS
                     KLFSRICSMVAQVSSQRIKRRELTEEDYFQLERKVDEVKKRRLSLIRASSMTVEDIRA
                     YTLAHRFDVIYVDYLTLIRAPGKTEFDQATYISKALHQMAQDLNITVVALSQLSRPEG
                     GKPKPPTLASLRSSGQIEQDADVVMFIFREEPGLLRSRRILRVAKNKEGETGQIPLVF
                     RGETQTFRPDSVGIMLPRTKEEPQYKQMEFGALEDPGEDTPDEFTKN"
BASE COUNT          330 a          737 c          619 g          371 t
ORIGIN      
        1 tgaccagcac cccggccacc cggatctccg gccggatata ccgcaggctg tcgatctgct
       61 ccatcaggtc ggccacgccg gtggcagagc aggcgtcgga cagcacgggg atgatgacgg
      121 cgtcgctggc caggatggcg gacacgcagg cggcggacag gttgggcggg cagtcgatga
      181 ccatcacgtc ataggctccg tcctcgtcca ccgcctcccg catgtcccgc agggtccggt
      241 agctccggcc accctcgccg ccggcggcct ccaggtccag ggcccacaga tcctccgacg
      301 ccgggaggac atccagcccc tccacgtcgg tatgtaccac cagctcgtca tagcagatag
      361 ccatgccccg catcagggcc ctcagcccgt cgtactcccc ccgtgggagc aggatctggg
      421 tggcgttggc ctgcccgtcc gcatccacca gcagcacccg caggtggcag ctggtagcca
      481 gcacataggc cagattcaca gcggtggtcg tcttccccac cccgcccttt cggttgacga
      541 ttgcaaatgt tctcatggtg ataactcctt tgtgtcaatt ttttgtgaac tcgtcggggg
      601 tatcctcccc tgggtcctcc agcgctccaa actccatctg cttgtactgc ggctcctcct
      661 tggtccgcgg gagcatgatc cccacggagt ctggccggaa ggtctgggtc tcgccacgaa
      721 acaccagcgg gatctgtcct gtctcgccct ccttgttttt ggccacccgg aggatacgcc
      781 ggctccgtaa cagccccggc tcctcccgga aaatgaacat gaccacgtcc gcgtcctgct
      841 cgatctgtcc ggaggagcgc agggaggcca gggtgggcgg ctttggcttc ccgccctccg
      901 gccgggacag ctgggacagg gccaccaccg tgatgttgag gtcctgggcc atctggtgca
      961 gggccttgga gatgtaagtc gcctggtcaa actcggtctt tcccggcgcc cggatcaggg
     1021 tcaggtagtc cacatagatc acgtcgaacc ggtgggcgag ggtataggca cggatgtcct
     1081 ccaccgtcat gctggacgcc cggatcaggg agagcctgcg ctttttgacc tcgtccacct
     1141 tgcgctccag ctggaagtag tcctcctctg tcagctcccg gcgcttgatg cgctggctgc
     1201 tgacctgggc caccatggag cagatgcggc tgaacagctt ggaggtcttg gtctccaggg
     1261 agaagaaccc cacccgcagt gtctccgcct gggcgtaggc catggacagc gccagcgcgg
     1321 tcttgccgtc cgacgggtag ccgcccagca ccacaaagtc tccgccctcc gccgtcagcc
     1381 cctcgtccag gaagtccatc ccccagggta gataccgggg cgtccgctcc agctctgcgt
     1441 agaagtccag catcccctgc tccatgtcca cgcactccac gccccgccgg tccatcatca
     1501 gctcatagag ccgcgacacc ggttcccggg ccgtctccag ggtgatatcc gtgccagcca
     1561 gggccagccc cgcccgctgg atctcccgga gccgggactg ctcccggaca atgtccatgt
     1621 actcccggta gttctgcgcc gtcggggtct cccgcatcaa ggccagcagg tacttccggc
     1681 gtcccgggtc cggcccgccc atctggttga gcacacacac cgggtcgatc ttctcccccg
     1741 ccaggtgcag ggcccggatg gccaggaaca cctcctgccc catgggctcg gtgaagtcct
     1801 ccggctggag gttggtcatc acgtcgccca cgatctgcgg ggagatcagc atggacccca
     1861 gcacgctgga ctgggccagc agatagtccg ccatcagtcc cacccccatc tctggggcgg
     1921 catggactgc tccgccgggc tcccgcccct gcgcagaggg aagactgatt tccagctgtt
     1981 ggccacagac tggcggatca gcagcagctt gtcctcccgg cggccctccg agagcctgtc
     2041 cagctcaccc aggagca
//
LOCUS       DBMDSJ010000326         2051 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12394_length_2051_cov_2.272821, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000326 DBMDSJ010000000
VERSION     DBMDSJ010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12394_length_2051_cov_2.272821"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="ACZPAQ_07685"
     CDS             complement(<1..260)
                     /locus_tag="ACZPAQ_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903908.1"
                     /translation="MEEHDNKKRTAVWLTPGVIRRMDSWLEEDNCKTRSEFIEKALRF
                     YMGYLATEDTSEYLSRALADTLRGTLADNENRLRTLLFKLCV"
     gene            complement(582..1397)
                     /locus_tag="ACZPAQ_07690"
     CDS             complement(582..1397)
                     /locus_tag="ACZPAQ_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903909.1"
                     /translation="MSVPVLTHRRDLRKGDLVFPEEILPIGHMLDVQLDCVGKEVQQE
                     VFGPELDPSPLCDWLNLHTAYDFALQDVVDHIEITLCRNFGEEEHPFTYPLNRAERDV
                     LRGEMEACCQLRTGRTLAQHYNHLLVQEEVEPPELTAGTRRIPVDKVSFADELSECGG
                     LLQFYMPVAFAPDAVFGTHVATAENDGWLNVYAAYDLNTGKPCSALDVILVCGDSSEF
                     AFRYPLAKQEQAALLEKMDAYCREQTGMALADFRARYLVEEPQPRQAPSLAQL"
     gene            complement(1394..1882)
                     /locus_tag="ACZPAQ_07695"
     CDS             complement(1394..1882)
                     /locus_tag="ACZPAQ_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903910.1"
                     /translation="MVWNRVPEEAGRGLMLWCDKEFLDVKVRSAVFTAEARGGRLWGV
                     AECQVVGQLSPEELTSLKDYLTGQAADGVGEGLEQQAIRVDGGELYVHLWQSEGWSIQ
                     TEGERFGQTQIQEPHDDVVPTEAQHNRFGGERSSGEMSEPCPQGEAKGMERGMTMGGM
                     SL"
BASE COUNT          378 a          717 c          555 g          401 t
ORIGIN      
        1 tccacgcaga gcttgaacag caaagtgcgc agccggtttt cattgtctgc caatgtaccc
       61 cgcagggtgt ccgccagggc gcgggagagg tactccgagg tatcctccgt ggccaggtag
      121 cccatgtaga accgcagggc cttttcaata aattcactgc gcgttttgca gttgtcctcc
      181 tccagccagc tgtccatccg gcggatcaca ccgggtgtca gccagacggc ggtgcgcttt
      241 ttgttgtcgt gttcctccat gttcttcacc tccgcttttt gcctgcgaca cagcggaacc
      301 cgctggcgca gtaagtgttt atctgaaccc gaccacccca aacggccaga atctcccatt
      361 ccgaccacct agcctgaaat ccgggaaagc cccacactgc gggactttgt gggcgggagg
      421 ccggcgcttg cgaccacctc cctttatcct cgtactaaaa tcgaacgccc caaaccgggg
      481 cgaaactggc cccaacgggg cagttttctg gctgggcggg gccctggccc gccctccgcc
      541 tgaacacgtc acacagggcg cacacggggc cgacggcatg ctcacagctg ggccagactg
      601 ggagcctggc ggggctgcgg ttcctccacc aggtaccggg cccggaaatc cgccaacgcc
      661 ataccggtct gctcccggca gtaggcgtcc atcttttcca gcagcgcggc ctgctcctgc
      721 ttggccagcg gataccggaa ggcgaactcg ctgctgtccc cgcacaccag gataacatcc
      781 agtgcagagc acggcttgcc ggtgttcagg tcataggcgg cgtacacgtt gagccagccg
      841 tcgttttctg cggtcgccac gtgggtgcca aagactgcgt ccggggcaaa ggccaccggc
      901 atatagaact ggagcagccc accgcactct gagagctcat ccgcaaaact caccttgtcc
      961 acggggatgc ggcgcgtccc agccgtgagc tccggcggct ccacctcctc ctgcaccagc
     1021 agatggttgt aatgctgtgc tagtgtgcga ccggtacgga gctggcagca ggcctccatc
     1081 tccccgcgca ggacgtcccg ctccgccctg ttgagcggat acgtgaatgg gtgctcctcc
     1141 tctccaaaat tccggcagag ggttatttct atatggtcca ccacatcctg aagcgcaaaa
     1201 tcataggcag tatggaggtt cagccagtca cacagtggac tgggatccaa ctccgggcca
     1261 aacacctcct gctgaacttc tttgccgaca cagtcgagtt gaacatccag catgtggcca
     1321 atgggcagga tctcctccgg aaacacaaga tcccctttcc tcagatccct gcggtgggtc
     1381 agcacgggaa cactcacaag ctcatccctc ccatcgtcat tccgcgctcc atacccttcg
     1441 cctcgccctg tgggcaaggc tcactcattt cgccggaact cctctcccca ccgaacctgt
     1501 tgtgctgggc ttcggtgggg accacgtcat cgtgcggttc ctggatctgt gtctgcccga
     1561 accgctcccc ctctgtctga atgctccagc cctcagattg ccacaggtgg acatagagct
     1621 ccccgccgtc cacacggatt gcctgctgct ccaggccctc gccaacgccg tccgcagcct
     1681 ggccggtgag atagtccttc aggcttgtca gctcctccgg agagagctgg ccgacgacct
     1741 ggcactccgc cacgccccac agcctgccgc ctctggcctc tgccgtaaac acggcggagc
     1801 gcaccttgac gtccaggaac tccttgtcgc accagagcat gaggccccgc cccgcctcct
     1861 ccggcacccg gttccacacc atgaacccag gatcctgtcc tcataatcca gaagcgttct
     1921 gccgtcccat tcctcacccg tctcatccca atctccccac ttattccggg catagaagct
     1981 ggcggtgagg ggcatgtaca gcttgagctg ggtggactca ggcgggtgga cggagaacat
     2041 ctcctcccca a
//
LOCUS       DBMDSJ010000327         2026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12563_length_2026_cov_1.322597, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000327 DBMDSJ010000000
VERSION     DBMDSJ010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12563_length_2026_cov_1.322597"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="ACZPAQ_07700"
     CDS             complement(<1..642)
                     /locus_tag="ACZPAQ_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR0903911.1"
                     /translation="MSDADKILAEMLEAVPDTYQKTVGFPTWDWLAAASIPIAQTEAE
                     LEEARRQLDPANLTGEALEAYIFPRTGQRRTAATCAVGNLTVTGNGTIPAGALFESGG
                     GVQFEATETVVIAGTGTVSIRCVTPGAAGDLPAESITMMPVQIAGIVSVTNEEPTAEG
                     YEAETDAAYYQRFLLRVQTPPTSGNQYHYLEWAMEVSGVGGVQVYPLDQGANTV"
     gene            complement(635..1069)
                     /locus_tag="ACZPAQ_07705"
     CDS             complement(635..1069)
                     /locus_tag="ACZPAQ_07705"
                     /inference="COORDINATES: protein motif:HMM:NF022387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HIR0903912.1"
                     /translation="MAMFFPMIPEAIPAQAAAEIGRVPAFDMEARRFLLVDGKLVERT
                     GQEAVRQWFELALRQQPDRVPIYRTSGEKRYGIDRGVLETKLPSGYAEAELERSVRET
                     ASFCPAVRAVRDLAISRQGRACLVEFTAVLHTGETVEVSVDV"
     gene            complement(1080..1361)
                     /locus_tag="ACZPAQ_07710"
     CDS             complement(1080..1361)
                     /locus_tag="ACZPAQ_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903913.1"
                     /translation="MTWDYELARRIRAGQERREAPAGLEGEVVRTDPLTVSLYGGEVM
                     APPAPLKVVEHAPGYTVVQHVIRKLSWNVGDRVVCCWMGKSVVILGRLT"
     gene            complement(1367..>2026)
                     /locus_tag="ACZPAQ_07715"
     CDS             complement(1367..>2026)
                     /locus_tag="ACZPAQ_07715"
                     /inference="COORDINATES: protein motif:HMM:NF048266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ/YqbQ family protein"
                     /protein_id="HIR0903914.1"
                     /translation="PVGEIPRLPTRLSEVWTGDAPETILQDILKICSAETGKQYKRRV
                     KGGALYVTELPDTPMTAYHKPADNLAAFDITLAKGTVSGSDSMGELRNSVVLVGGGQT
                     EVLGRGYNQASIDEYGLVQTVERLSGDENSAQARQRIRTLLDQNDRLTIERQVEEIWG
                     ADEVESGVLLRFQSNRFGVTGDQRVVGVTHRYGPTHLMSLTVQDPAQGRAADAKDTVE
                     V"
BASE COUNT          286 a          790 c          579 g          371 t
ORIGIN      
        1 caccgtgttg gccccctggt ccagggggta cacctggacg ccgcccaccc cggacacctc
       61 catggcccac tccaggtagt ggtattggtt cccgctggtg ggcggggtct gcacccggag
      121 caggaaccgc tggtagtagg cggcatccgt ctccgcctcg tatccctccg ccgtgggctc
      181 ctcgttggtc accgacacga tgccggcaat ctgcaccggc atcatggtga tgctctccgc
      241 cggcagatca ccggccgccc ccggcgtcac gcaccggatg gacacggtcc ctgtccccgc
      301 gatgaccacc gtctccgtgg cctcgaactg gaccccgccg ccgctctcaa acagcgcccc
      361 ggccgggatg gtcccatttc ccgtgaccgt caagttgccc acggcgcagg tggccgccgt
      421 ccgccgctgt cctgtcctgg ggaagatgta cgcctccagg gcctctccag tcaggttggc
      481 cgggtccagc tgacgccggg cctcctccag ctccgcctct gtctgagcaa tggggatgga
      541 ggccgccgcc agccagtccc aggtggggaa gcccaccgtc ttctggtagg tatccggcac
      601 cgcctccagc atctctgcca gtatcttgtc tgcgtcagac atccacgctc acctccactg
      661 tctctcccgt atgcaatacc gccgtaaatt ccaccaggca ggcccttccc tgccgggaga
      721 tggcgagatc ccggactgcc cgcaccgctg ggcagaagct ggcggtctcc cgcacgctgc
      781 gctccagctc cgcctccgcg tagccggagg gcagctttgt ctccagcacc ccccggtcga
      841 tgccgtaccg cttctccccg ctggtgcggt agatgggcac ccggtccggc tgctgccgca
      901 gggccagctc gaaccactgc cgcactgcct cctgtcccgt gcgctccacc agctttccat
      961 ccaccagcag gaaccggcgc gcctccatat caaaggcggg cacacgcccg atctccgccg
     1021 ccgcctgggc tggaatggcc tccgggatca tcggaaagaa cattgccatc tctctcacct
     1081 cacgtcaatc ggcccaatat gactacgctt ttccccatcc agcagcacac tacccggtcc
     1141 cccacgttcc aggacagctt cctgatgacg tgttggacga ccgtgtaccc cggggcgtgt
     1201 tccaccacct tcaggggggc cggcggtgcc atcacctctc cgccgtacag ggagacggtc
     1261 aggggatcgg tgcgcaccac ctctccctcc agccccgcgg gggcctccct gcgctcctgt
     1321 cccgcccgga tgcgccgggc cagctcataa tcccacgtca tgtcccttac acctccacag
     1381 tgtccttggc gtccgccgcc cggccctgag ccgggtcctg gacggtcagg ctcataaggt
     1441 gggtgggacc gtaccggtgg gtcacgccca ccacccgctg gtcccctgtg acgccgaatc
     1501 ggttgctctg gaagcgaagc agcaccccgc tctccacctc gtccgccccc cagatctcct
     1561 ccacctgccg ctcaatggtc agccggtcgt tctgatccag cagggtgcgg atacgctggc
     1621 gggcctgggc gctgttctcg tccccgctca ggcgctccac cgtctggacc aggccatact
     1681 cgtctatgct ggcctggttg tacccccggc ccagcacctc cgtctggccg ccccccacca
     1741 ggaccacgct gttccgcagc tcccccatgc tgtccgatcc gctcacggtc cccttggcca
     1801 gcgtgatgtc gaaggcggcc aggttgtccg ccggcttgtg gtaggccgtc atgggggtgt
     1861 cgggcagctc cgtcacatac agcgccccgc ccttgacccg ccgcttatac tgctttccgg
     1921 tctccgcgga gcatattttc aggatgtcct gcaaaatggt ctccggtgca tcccccgtcc
     1981 acacctcgga cagccgggtg gggagcctgg ggatctcccc caccgg
//
LOCUS       DBMDSJ010000328         2025 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12575_length_2025_cov_1.211538, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000328 DBMDSJ010000000
VERSION     DBMDSJ010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12575_length_2025_cov_1.211538"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="ACZPAQ_07720"
     CDS             complement(<1..317)
                     /locus_tag="ACZPAQ_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 C-terminal domain-containing protein"
                     /protein_id="HIR0903915.1"
                     /translation="MVEIISVRFQSGGKQYYFNPDGRQFQIGDGVIVETSRGTEYGEC
                     VQANSMIDEIELTAPLRPVIRVATEEDRQTVEANREKEKKAFSICQQKIAEHGLDMKL
                     VKA"
     gene            complement(401..1360)
                     /locus_tag="ACZPAQ_07725"
     CDS             complement(401..1360)
                     /locus_tag="ACZPAQ_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024575.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR0903916.1"
                     /translation="MFPQVRKVMEFSALAGNDRLKEQLSQQESGRGLAHAYIISGPTG
                     SGRHTLARLLAAAMVCTSDSGSRPCGKCSPCKKITAGIHPDFEIISGPEEGKPITVDQ
                     IRALRSDAYIRPNEGNRKIYLLEGADQMNASAQNAMLKLLEEGPGYAVFLLLAENGGG
                     LLQTVRSRCEELVLSPVSVVQCQSWLKDRFPQKSLEEIQTAALDCQGVLGRAVDRLSG
                     TDEKTRTREEQADRLAQAVEEGTELQLFEAAMVLDRMDREELNRLLDSLEAALGARIG
                     RSGDRRRSLRGVELVHQLRRASQLNANSGQMAGWLCAGMFIKE"
     gene            complement(1367..>1493)
                     /locus_tag="ACZPAQ_07730"
     CDS             complement(1367..>1493)
                     /locus_tag="ACZPAQ_07730"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015656.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Orn/Lys/Arg family decarboxylase"
                     /protein_id="HIR0903917.1"
                     /translation="IAPYPPGVPVVAPGEVISKRSLAYLKQIGYNIKSEVRIAAL"
     assembly_gap    1494..1503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1504..>2025)
                     /locus_tag="ACZPAQ_07735"
     CDS             complement(<1504..>2025)
                     /locus_tag="ACZPAQ_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015656.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0903918.1"
                     /translation="GALLFTNGMDPDRVRQLASVFGTSSPSYPIMASMDTAREWMVKW
                     GHHDYRRTALRVAQLREQFPSLREKRLSLDPTRFVLSVQNGPAFTRALEKRGVYPEME
                     DGGHVVFICTPFDNSFSLSALERRLEELREQMGPCPPIPAPPLPEQAMSPRQALFAPS
                     RVWPLEDCEGEVA"
BASE COUNT          382 a          657 c          543 g          433 t
ORIGIN      
        1 tccgccttga ccagcttcat atccaggcca tgctcggcaa tcttctgctg gcagatggaa
       61 aaggctttct tctccttctc tcggttggcc tctactgtct gccgatcctc ctccgtggcc
      121 acccggatca ccgggcgcag gggggcggtg agctcgatct catcgatcat agagtttgct
      181 tgtacacact caccgtactc cgtacccctg gaggtctcca cgatgacccc gtcccctatc
      241 tggaactgcc gtccatcggg gttgaaataa tattgctttc cgccgctttg aaagcggacg
      301 ctgatgatct ctaccatagg ggattctcct tcttctctcc agagtggata cgcggggccg
      361 gcggctgtgc cgccttttcc ccgctccgct cccggagttc tcactccttt atgaacatcc
      421 cggcacataa ccagccagcc atctgaccag aattggcgtt gagctgtgaa gccctccgca
      481 gctgatggac cagctctact ccccggaggg atctacggcg gtcgccgctc ctaccaattc
      541 gggcacctag agccgcctcc agactgtcca gcagtcggtt cagctcctcc cggtccatcc
      601 tatccaaaac catggccgcc tcaaagagct gcagctccgt accttcctcc acggcctggg
      661 ccagccggtc cgcctgctcc tctctggtcc tggttttctc gtcggtccca gacagccggt
      721 ccaccgcccg gcccagaaca ccctggcaat ccagggcggc tgtctggatc tcctccaagc
      781 ttttttgggg aaagcggtcc ttcagccagc tctggcactg gaccacagaa acaggggaca
      841 gcaccagttc ctcgcaccgg gagcgcactg tctgaagcag tccccccccg ttctccgcca
      901 acagcagaaa gacggcatat ccagggccct cctccaacag cttcaacatg gcattctggg
      961 cggaggcatt catctggtct gccccttcca gcagatagat tttccgattc ccctcgttag
     1021 gacgtatata tgcgtcgctt cggagggctc ggatctgatc cacggtgata ggtttgccct
     1081 cctccgggcc ggaaataatc tcaaaatctg gatggattcc agcggttatc tttttgcatg
     1141 gggagcattt tccacagggc ctgctgccgc tatcggacgt acataccata gcggcggcca
     1201 gcagccgggc caaagtatgc cggccggagc cggttggacc tgaaataatg taggcgtggg
     1261 ccaggccgcg gccgctctcc tgctgggaga gctgctcctt caacctgtcg ttgccagcca
     1321 aggccgaaaa ttccataacc ttcctcactt gtgggaacat tctccatcag agggcggcaa
     1381 tccgcacctc tgattttata ttatatccta tttgtttcaa ataggccagc gatctttttg
     1441 agattacctc gccgggagcc actactggta ctcccggagg gtaaggcgca atcnnnnnnn
     1501 nnncggccac ctccccctca cagtcctcca gcggccagac ccggctgggg gcaaagagag
     1561 cctgccgggg agacatggcc tgctccggca gaggcggagc tggaatgggc ggacagggtc
     1621 ccatctgctc ccgcagctcc tccagccgcc tctccagcgc actcagggaa aagctgttat
     1681 caaagggggt acagataaag accacatggc ccccgtcctc catctctgga taaacgccgc
     1741 gcttctccag cgcgcgggta aaagcgggac cgttctggac gctgagaaca aagcgggtgg
     1801 gatccagtga cagtcttttt tctcttaggg aggggaattg ctcccgcagc tgtgccaccc
     1861 ggagagcggt ccggcggtag tcgtgatgtc cccatttgac catccactct cgggccgtgt
     1921 ccatgctggc cataatggga taggagggac tggaggtgcc aaagaccgag gccaactgcc
     1981 gcacccggtc cgggtccatc ccattggtaa agagcagggc ccctt
//
LOCUS       DBMDSJ010000329         2023 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12588_length_2023_cov_1.739334, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000329 DBMDSJ010000000
VERSION     DBMDSJ010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12588_length_2023_cov_1.739334"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(80..1168)
                     /locus_tag="ACZPAQ_07740"
     CDS             complement(80..1168)
                     /locus_tag="ACZPAQ_07740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0903919.1"
                     /translation="MKQVVCLSTEPWSPTPGRTQHLITRLKDAQVLYFCPGGGLLDQR
                     WRQPGRKVRPNVTVYTMPPALPVDERHDRLFRLSRQRQIRFLADKLARHRFRRPLLWT
                     TSPVHIHALDALEYDGLVYDCDQVWDELPDRWEGSLAGAADVVFAASPGLADRLSPCR
                     GNIALLPNGVNYPLFAAAGSAGRRGELDHLPGPLLGWAGTIHADLDLTPILYAAQARP
                     AWTFLLLGRREENPLLRRLSRLSNVMLAGPCPLVEVPDYLGRCQVLLDLLRDSQPYND
                     VVPPRLYEYLSTGKPIVSMLWPEQVELFPDVVYGAHSPEEFLHLCQQALAEDPGWVDQ
                     RRRDYGAAAAWSRRADEVKRILVTAGLL"
     gene            1375..1893
                     /locus_tag="ACZPAQ_07745"
     CDS             1375..1893
                     /locus_tag="ACZPAQ_07745"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR0903920.1"
                     /translation="MLWIQENLRGLLDPIVEGYTTLGNTGLMWIVLSLAMLCWKPTRK
                     AGVAALAAMVLGLLCTNVVLKHVVARPRPWLDVAGLVPLIQEPDPNSFPSGHTCAAFA
                     AASAWWRALPRRWMRWTGLVLAVCMGLSRLYVGVHYPSDVIAGALVGLFCGWAAWKLL
                     QLWEERRGSRLR"
BASE COUNT          356 a          594 c          746 g          327 t
ORIGIN      
        1 gggcagggca tttttgtcag cttccacaag gagcgggaga aaaaatgaca gggaagtcgt
       61 caggacggcg ggggcgggct cacagcaggc ccgccgtgac caggatgcgc ttgacctcgt
      121 cggcccgccg tgaccaggcg gcggccgcac cgtagtcccg gcggcgctga tccacccagc
      181 ccgggtcctc cgccagcgcc tgctggcaca gatggaggaa ctcctccggg gagtgggcgc
      241 cgtagaccac atcggggaag agctccacct gttccggcca cagcatggat acgatgggct
      301 tgccggtgga gagatactca tagagccggg gcgggaccac gtcgttatag ggctggctgt
      361 cccgcagcag gtccagcaga acctggcagc ggcccaaata gtcggggacc tccaccagcg
      421 ggcaggggcc ggccagcatc acattcgaca ggcgggagag acggcgcagc agagggtttt
      481 cttcccggcg ccccagcagc aggaaggtcc aggcgggccg ggcctgggcg gcatagagga
      541 tgggggtcag atccaggtcg gcatggatgg tgcctgccca gcccagcagg ggcccgggca
      601 ggtggtccag ctccccccga cgcccggcgg agcccgcggc ggcgaagaga gggtaattca
      661 ccccgttggg aagcagcgcg atgttgccgc ggcacgggga gaggcggtcg gccagccctg
      721 gggaagcggc aaagaccacg tccgccgcac cagccagact gccctcccac cggtcgggca
      781 gctcgtccca cacctggtcg cagtcgtaga ccagaccgtc gtactccagc gcatccaggg
      841 cgtggatatg gacaggggag gtggtccaca gaaggggacg gcggaagcgg tgccgggcca
      901 gcttgtcggc gagaaagcgg atctggcgct gccgggagag ccggaacagc cggtcgtggc
      961 gctcatccac agggagggcg gggggcatgg tatagacggt gacgttgggg cggaccttcc
     1021 tgcccggctg gcgccagcgt tgatccaaca gtccgccgcc ggggcagaag tagaggacct
     1081 gggcgtcctt gagccgggtg atcaggtgct gggtccggcc gggggtgggg ctccagggct
     1141 cggtggagag acagacgact tgcttcaaaa gggaggacct cccgtgttct catgatctaa
     1201 caaaaacggc ttgcctcggg cccgggagca gggtataata tccctgccgg ccggtcgggc
     1261 tttgaccggt catatctcac tttattatag tcccggagcc ccggcgggtc aagggaagga
     1321 aaggcaggaa gtttttccat gttatccgtg cttcagacat tggatggagg ggccttgctc
     1381 tggatccagg agaacctgcg gggactgttg gaccccatcg tggagggata tactacactg
     1441 ggcaacaccg gcctgatgtg gatcgtgctc tctctagcca tgctgtgctg gaagcctacc
     1501 cggaaagcgg gggtggcggc cctggcggcc atggtgctgg ggctgctgtg taccaacgtg
     1561 gtcctcaagc atgtggtggc ccgcccccgc ccctggctgg atgtggcggg gctggtgccg
     1621 ctgatccagg agccggaccc caactccttt ccctcagggc atacctgtgc tgcctttgcg
     1681 gcagcatccg cctggtggcg ggcgctgccc aggagatgga tgaggtggac cgggctggtg
     1741 ctggcggtgt gcatgggcct gtcccgcctg tatgtggggg tccactaccc cagcgatgtg
     1801 atcgcagggg cactggtagg cctgttctgc ggctgggcgg cctggaagct gctgcagctg
     1861 tgggaggagc ggcggggatc taggctccgc tgaattgcca tagaggagcg cagaagcgcc
     1921 gcccggcatc taaacagatg ccggacggcg cttttctctt gggagttcat ccccgctttc
     1981 gcagcaggaa ggtgaccaga aagatgccgg ccagatacag ggt
//
LOCUS       DBMDSJ010000330         2018 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12632_length_2018_cov_1.540428, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000330 DBMDSJ010000000
VERSION     DBMDSJ010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12632_length_2018_cov_1.540428"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="ACZPAQ_07750"
     CDS             <1..262
                     /locus_tag="ACZPAQ_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903921.1"
                     /translation="FGDSLTQGGDVHRLYVTLGQFSIIRLERDTQLLMPVYDYCMPEK
                     ECDCGSDCSQEDPCEVFRQVKFPVDEFFPPNSSCSRNCSRSC"
     gene            371..847
                     /locus_tag="ACZPAQ_07755"
     CDS             371..847
                     /locus_tag="ACZPAQ_07755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR0903922.1"
                     /translation="MRRSDRARDRSFAFDVIDRCGHGVAALSTGGEGPYCIPLSLVRV
                     GDDLFFHCALEGRKLDLLRRDPRVCVTFVAADQPAYLPESNMYTTFFQSAVVTGTAFE
                     VTDPGQKTAALRALCEKMTPGHMEGFEEAVRRSLPSTAVWGIHMEEVTGKEKARKR"
     gene            complement(907..>2018)
                     /gene="cls"
                     /locus_tag="ACZPAQ_07760"
     CDS             complement(907..>2018)
                     /gene="cls"
                     /locus_tag="ACZPAQ_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR0903923.1"
                     /translation="PAYTNTRTEYFPVGDLAFVRMLEELEKAERYIFLEYFILQEGEC
                     WNAILDILERKAAAGVEVRLIYDDFGCMFTLPRTYNDQMAARGIQCRVFNRLVPVLTL
                     RMNNRDHRKLCIIDGKVGFTGGMNLADEYINRRVRFGHWKDSAILLRGEAVWSMTVMF
                     LSMWDYCSGWEEDFDLFRPESMPRLPGSGWVQPYTDTPLDHEKVGQAVYLNMISRARD
                     YIYITTPYLIVDVATNTALCNAAKAGVDVRIITPHIPDKRSVFEVTKAHYPPLLEAGV
                     KIYEYTPGFIHAKNFVVDGRYATVGTVNLDYRSLFLHFEDGVWLCDAPCIADIKADFE
                     KTLEQCEAISLRRARHLNILVQLYRSVLRIFAPLM"
BASE COUNT          372 a          666 c          621 g          359 t
ORIGIN      
        1 cttcggggac tccctcaccc agggcggcga cgtacaccgc ctgtacgtca ccctgggcca
       61 gttctccatc atccgcctgg agcgggacac ccagctgctc atgccggtct acgactactg
      121 tatgccggag aaggagtgcg actgcggcag cgactgcagc caggaggacc cctgcgaggt
      181 cttccggcag gtcaagttcc ccgtggacga attcttcccg cccaacagca gctgcagccg
      241 caactgctcc cgcagctgct gagcccccca aaacctcccc tcctgccgga ggggaggttt
      301 ttcttgcagt ccctcccaaa ttgtgctatg gtagctccag tctatttgat gcagaaaaga
      361 ggagatcctg atgagaagat cagacagagc ccgggaccgg agctttgcct ttgacgtgat
      421 cgaccgctgc gggcacggcg tcgccgccct ctccaccggc ggagagggcc cctactgcat
      481 ccccctctct ctggtgcggg tgggggacga cctgtttttc cactgcgccc tggagggccg
      541 caagctggac ctgcttcgcc gggaccccag ggtgtgcgtc acctttgtgg ccgccgacca
      601 gcccgcctac ctcccggaga gcaacatgta caccaccttc ttccagagcg ccgtggtcac
      661 cggcaccgcc tttgaggtca cagaccccgg ccagaagacc gctgccctgc gggccctgtg
      721 tgaaaagatg acccccggac atatggaggg ctttgaggag gccgtccgcc gcagcctgcc
      781 gtccaccgcc gtctggggca tccatatgga ggaggtcaca ggcaaggaga aggcacggaa
      841 gcggtaaaga tcccggaagc ctcaaaagcc tcggcgcccc gatgattcgg agcgccgagg
      901 cttttctcac atcagtgggg cgaagatgcg caggacgctg cgatagagct ggaccaggat
      961 gttcaggtgc ctggcccgac gcaggctgat ggcctcgcac tgctccaggg tcttctcaaa
     1021 gtccgccttg atgtccgcga tgcagggggc atcacacagc cagacaccgt cctcaaagtg
     1081 gaggaagagg ctgcggtagt ccaggttcac ggtgcccacc gtggcatacc gcccgtccac
     1141 cacaaagttt ttggcgtgga tgaagcccgg cgtgtactcg tagatcttca cccccgcctc
     1201 cagcagaggc ggatagtggg ccttggtcac ctcgaacacg gagcgcttgt ccgggatgtg
     1261 gggggtgatg atgcgcacat ccacccccgc tttggcggcg ttgcacaggg ccgtgttggt
     1321 ggccacgtcc acgatcagat agggcgtggt gatatagata tagtcccggg cccgggagat
     1381 catgttcagg tacaccgcct gccccacttt ctcgtggtcc aggggcgtat ccgtgtaggg
     1441 ctggacccag ccggagccgg ggagccgggg catggactcg ggccggaaca ggtcgaagtc
     1501 ctcctcccag ccggagcagt agtcccacat ggacaggaac atgacggtca tggaccacac
     1561 cgcctccccc cgcaggagga tggcgctgtc cttccagtgg ccgaaccgca cccggcggtt
     1621 gatatactcg tccgccaggt tcatcccccc ggtgaagccc accttcccat cgatgatgca
     1681 cagcttccgg tggtcccggt tgttcatccg cagggtcagc accgggacca gccggttgaa
     1741 cacccggcac tggatgcccc gggccgccat ctggtcatta taagtgcggg gcagggtgaa
     1801 catgcagccg aagtcgtcat agatcagccg cacctccacc ccggcggcgg ccttccgctc
     1861 caggatgtcc aggatggcgt tccagcactc cccctcctgg aggatgaaat actccaggaa
     1921 gatgtaacgc tccgccttct ccagctcctc cagcatccgc acgaaagcca ggtcgcccac
     1981 cgggaaatat tcggtccggg tgttggtgta ggcggggc
//
LOCUS       DBMDSJ010000331         2013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12674_length_2013_cov_1.246862, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000331 DBMDSJ010000000
VERSION     DBMDSJ010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12674_length_2013_cov_1.246862"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="ACZPAQ_07765"
     CDS             complement(<1..457)
                     /locus_tag="ACZPAQ_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR0903924.1"
                     /translation="MERGSGHNDKLDLWKCVAIYSVIFIHILLPGQIGVAVNCLARFA
                     VPLFFLSAGFFSWRSPPRVLARRTVRTAGLLLFACLALLGLGCAMAKHGGASMGAYLL
                     GRFTWANAASLVLWQLFPLPYSWPLWFLAALLMIYLMWWGLTALLLGRLP"
     gene            810..1352
                     /locus_tag="ACZPAQ_07770"
     CDS             810..1352
                     /locus_tag="ACZPAQ_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR0903925.1"
                     /translation="MRKFTTRDLTLAAVLAAVYAALTITLPVPQYGAVQIRFAEALTV
                     LPFLFPAATPGLFVGCIIANLFSPFVLDVVFGSMATLLACLWTSRMTSRWLAPLPPVL
                     CNAVIVGAEIAWAETGITPAFWTAFGFNAVTVGLGELIACYALGSLLLSAMPQVPYFR
                     GMIPAERLERLSSRAGRERA"
     gene            1482..>2013
                     /locus_tag="ACZPAQ_07775"
     CDS             1482..>2013
                     /locus_tag="ACZPAQ_07775"
                     /inference="COORDINATES: protein motif:HMM:NF020075.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR0903926.1"
                     /translation="MRFQGRDAARGRRMRKDLGRTAGAGVALALVLFLMPALLVRQGE
                     FSQELLPQGTLPLHRTPSSVSAASQDRGRVVRLSRSDGTVEELTMEEYLWGVVAAEMP
                     ASFEEEALKAQACAARTYTVTLQEASSPRHPDAQICDDSGCCQAYLDRAEAEARWGAN
                     AELYSGKIQRAVEETDG"
BASE COUNT          369 a          603 c          682 g          359 t
ORIGIN      
        1 ggggcaggcg gcccaggagt agcgcggtga ggccccacca catcaggtag atcatcagca
       61 gcgcggccag aaaccacagc ggccaggagt agggcagggg aaagagctgc cacagtacca
      121 gcgatgctgc gttggcccag gtaaagcgcc ccagaaggta ggcacccatg gaggcaccgc
      181 cgtgtttggc catggcgcac ccaagaccca gcagggcgag gcaggcgaag agcagcaggc
      241 cggcggtgcg gaccgtccgg cgggccagca cacggggcgg actgcgccag ctgaagaagc
      301 cggcggagag gaaaaacagc gggaccgcaa accgggccag gcagttcacc gccaccccga
      361 tctgtcccgg aaggaggata tggataaaga tgacgctgta aatggcgaca catttccaca
      421 ggtccagctt gtcgttgtgt ccgctcccgc gctccatgcg cctcacctcc tctggcctgt
      481 aacgtaacat tatatcacag ccgcccggga aagaaaagcc gagtctgtcc cgatcttcct
      541 ccctctctcg ttgagttgtt gtccacaact tcaactttaa ccgatgaggc attgtcctcc
      601 atccttttta ttcatctgtc cagcatgtat tgtactccta cagtaggcga acgaccgggg
      661 gcaaaatttt tctttacagg ggaagcgggc tgtggtatga tgccggggac gagttcccac
      721 atctcgtcca agcacggtcg tgagacgcac cgtgttcgtt cgggaggccc tgcctctcgt
      781 tctctaaaaa agaaaatgga ggttttttta tgcgcaaatt taccacccga gacctgaccc
      841 tggccgctgt cctggccgct gtctacgcag cgctcaccat cactctgccc gtgccacagt
      901 acggagcggt ccagatccgg ttcgccgagg ccctcaccgt gctcccgttc ctgttccccg
      961 ccgccacgcc ggggctgttt gtggggtgca tcatcgccaa cctgttttcc cccttcgtgc
     1021 tggatgtggt gttcgggtcc atggccaccc tgctggcctg cctgtggacc agccggatga
     1081 ccagccgatg gctggcaccc ctgccccccg tgctgtgcaa cgcagtgatc gtgggggcgg
     1141 agatcgcctg ggcggagacc ggcatcactc ccgcgttttg gacggcattc ggcttcaacg
     1201 ccgtgacggt ggggctgggt gagctgatcg cctgctatgc tctgggctcc ctgctgctgt
     1261 ccgcaatgcc tcaggtgccc tatttcaggg ggatgatccc ggccgagcgg ctggagcggc
     1321 tgtcttcccg tgcggggcgg gagcgggcct gaagatagaa cgagaaagag atcaccggtc
     1381 gggagagccg tccggtgatc tctttttgcg catgtttggg gtgggacatg cataatctgg
     1441 agggtgtgag agcaatcagg ggaaaggcgg gagggcggga catgagattc caggggcgag
     1501 acgccgccag ggggcggagg atgcggaagg atctgggccg gacggccgga gcgggggtgg
     1561 ccctggctct ggtcctgttc ctgatgcccg ccctgctggt gcggcagggg gaattttccc
     1621 aggagcttct gccccaggga acactgcccc tccatcggac gccgtccagc gtctcggccg
     1681 ccagccagga ccgggggcgg gtagtgcggc tgagccgatc ggacgggaca gtggaggagc
     1741 tgaccatgga ggagtacctg tggggggtgg tggcagcgga gatgcccgct tcctttgagg
     1801 aggaggccct gaaggcacag gcctgcgccg cccgtaccta tacggtcacc cttcaggagg
     1861 catccagccc ccggcacccg gacgcccaga tctgcgatga cagcggctgc tgccaggcgt
     1921 atctggaccg ggcggaggcg gaggcccgct ggggggccaa tgcggaactg tacagcggaa
     1981 agatccagcg ggccgtggag gagaccgatg gac
//
LOCUS       DBMDSJ010000332         2009 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12709_length_2009_cov_1.569182, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000332 DBMDSJ010000000
VERSION     DBMDSJ010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12709_length_2009_cov_1.569182"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="ACZPAQ_07780"
     CDS             <1..394
                     /locus_tag="ACZPAQ_07780"
                     /inference="COORDINATES: protein motif:HMM:NF027796.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HIR0903927.1"
                     /translation="GSVGEMAAQADHTVFGAVMFVMSICILAGIIINIFAVEKIQTGM
                     STLKKKRGDWGTIVNSCFFLGIVAAFTPLIFASGTVYALTFLISMGVAALCALCMRRF
                     HLKWLGSFIMSISLIVSMAASVLLEQIF"
     gene            413..1126
                     /locus_tag="ACZPAQ_07785"
     CDS             413..1126
                     /locus_tag="ACZPAQ_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903928.1"
                     /translation="MKQSESYDRSIHRTGRLGSILAIAWMLGIPAVMCTVYHMFPSIP
                     DLLLFGSGLLALYVPTAISEVITYVPMLGSASYITFITGNISNLKLPCALNALEMADV
                     EQNTDAGDAVATVAVAVSSIVTTVIIVLGVLLLVPLQPILESEPVKIAGSYMLPALYG
                     CLFLSQMTSKAGGTVIKGKLKAAVLPGVLVFLIHVFVTPVNRGIAILACIPLCILCAW
                     VLYRMGQIRVVHLPQGGDR"
     gene            1148..>2009
                     /locus_tag="ACZPAQ_07790"
     CDS             1148..>2009
                     /locus_tag="ACZPAQ_07790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR0903929.1"
                     /translation="MNYLQSAQALYEQMVRDRRHLHQHPEVGMDLPETCSYVMARLTE
                     MGYQPRRLGGGVTATVTGKPDGKVFLLRPDMDALPMREESGLPFASQRSCAHTCGHDM
                     HTAMLLGAAQLLRDRAAELNGTVKFMFQPGEEVLSGARSMIEAGILEDPHVDAAMGAH
                     MIPMIPAGLGGYGTGVVSASSDHLVIRVEGRGGHGAHPQSTVDPINIGVHIHLALQEL
                     LSREADPAELVVLTFGKFQGGDAANIIPSSAVLEGTLRTFSEPLRTHLLERIDTICTC
                     TAEAFRGRAQV"
BASE COUNT          346 a          678 c          601 g          384 t
ORIGIN      
        1 cggctccgta ggcgagatgg ccgcccaggc cgaccacacc gtcttcggcg cggtcatgtt
       61 cgtaatgagc atctgcatcc tggccggcat catcatcaac atcttcgcgg tagaaaagat
      121 ccagactggg atgagcaccc tgaagaaaaa gcggggcgac tggggcacca tcgtcaactc
      181 ctgcttcttc ctggggatcg tggccgcctt cacgccgctg atcttcgcct ccggaacagt
      241 ttacgccctg accttcctca tcagcatggg agtggcggcc ctgtgcgctt tgtgcatgag
      301 gcgctttcat ctcaagtggc tggggagctt catcatgtcc atctctctga tcgtcagcat
      361 ggccgcgtcc gtcctgctgg aacagatatt ttaaaaggag gctcgttctc acatgaagca
      421 gtctgaaagc tacgaccgct ccatccaccg cacgggccgg ctgggcagca tcctggccat
      481 cgcctggatg ctgggcatcc ctgcggtgat gtgcaccgtc tatcacatgt tcccttccat
      541 cccggacctg ctgctgttcg gcagcggcct gctggccctg tatgtcccca ccgccatctc
      601 agaggtcatc acctacgtcc ccatgctggg ctccgcctct tacatcacct tcatcaccgg
      661 gaacatctcc aacctgaagc tcccctgcgc cctgaacgcc ctggagatgg cggatgtgga
      721 gcagaatacc gacgccggag acgcggtggc cacggtggcg gtggccgtct cctctatcgt
      781 aaccaccgtt atcatcgtac tgggcgtcct cctcctggtc cctctccagc ccattctgga
      841 gagcgagccg gtgaagatcg ccggaagcta tatgctcccg gcgctttatg ggtgcctgtt
      901 cctcagccag atgaccagca aggcgggcgg cactgtcatc aagggcaagc tcaaggccgc
      961 cgtcctcccc ggcgtgctgg tctttctgat ccacgtgttc gtcacaccag tcaaccgcgg
     1021 catcgccatc ctggcctgca tccccctgtg catcctgtgc gcctgggtgc tgtaccggat
     1081 ggggcagatc cgggtggtcc atcttcccca gggtggggac cggtgaccgg aaaggagagt
     1141 acatcgtatg aattatcttc agagcgctca ggcgctgtat gaacagatgg tccgggaccg
     1201 ccggcacctc caccagcatc ccgaggtggg gatggacctg cccgagacct gttcctatgt
     1261 catggcacgg ctgaccgaga tgggctatca gccccggcgg ctgggagggg gcgtcaccgc
     1321 caccgtgacc ggaaagcccg acgggaaggt gttcctgctc cggcccgata tggacgctct
     1381 gcccatgcgt gaggagagcg ggctcccctt cgcctctcag cgcagctgtg cccacacctg
     1441 cggccacgac atgcacaccg ccatgctgct gggggcggcc cagctgctcc gggaccgggc
     1501 agcagagctc aacggcactg tcaagttcat gttccagccc ggcgaggagg tcctgtcagg
     1561 ggccagatcc atgattgagg ccggcatcct ggaagatccc catgtggatg cggccatggg
     1621 tgcccacatg atccccatga ttccagcggg cctgggcggc tacggcaccg gcgtggtctc
     1681 cgcctccagt gaccacctgg tcatccgggt ggagggccgc ggaggccacg gcgcccatcc
     1741 ccagtccacc gtggacccta tcaacatcgg cgtccacatc catctggcgc tccaggagct
     1801 gctgagccgg gaggcggacc ccgctgagct ggtggtcctc accttcggga agttccaggg
     1861 gggagacgcg gccaacatta tcccctcctc tgcggtgctg gagggcaccc tgcgcacctt
     1921 cagcgagccg ctgcgcaccc acctgctgga acggatcgac accatctgca cctgcacagc
     1981 ggaggccttc cggggcagag cccaagtca
//
LOCUS       DBMDSJ010000333         2003 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12757_length_2003_cov_1.460042, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000333 DBMDSJ010000000
VERSION     DBMDSJ010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12757_length_2003_cov_1.460042"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="ACZPAQ_07795"
     CDS             <1..494
                     /locus_tag="ACZPAQ_07795"
                     /EC_number="5.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR0903930.1"
                     /translation="LLEECGCTVIAIPCNTSHYFVDRIQQEIGIPILHMIRETARTLV
                     AQGKRRPAILATDGTIRTGLYQKECAAFGLEAAPPEPAVQRLVMSIIYEEIKRGERGS
                     REKFAQIDRALASMGCDCAILGCTELSVFRSYHSLPPFYVDAMEVLAELAVVRCGKQL
                     RTI"
     gene            608..>2003
                     /locus_tag="ACZPAQ_07800"
     CDS             608..>2003
                     /locus_tag="ACZPAQ_07800"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR0903931.1"
                     /translation="MDLTPHTLGEYCALLEPQLAAPLPEGLDLSQSVALVSCDSQEVV
                     PGTLFLCKGAHFKEAYLSQAAQRGAVAYVSQTPYPAVDLPCILVSDMRQVMAPLADRY
                     YGHPSGLLSVIGITGTKGKSSTAYYLKYILDEYLAEQGGPESGIISSIDTYDGSERFE
                     SHLTTPEPLDLQRHFAHAVAAGIPYLTMEVSSQALKYHRTLCTRFAAACFLNIGLDHI
                     SPIEHPDFEDYFSSKLRIFAQAEVGCVNLDCEHAGRVLAAAQAGSRQVITFSQKDPEA
                     DIYASQVRKRGNDILFRVRSRRFSREFRLTMPGLFNVENALAAIAVCEGLNIPERAVY
                     VGLMKARVPGRMEVYTNADSHIVAIVDYAHNRMSFETLFRSVQTEYPGRRVVTVFGCP
                     GKKALDRRRDLGEISGRCSDLVVLTEEDSGEEDTLSICREIASYVEAQGCDYSIEPNR
                     GEAIRQAVLGCREPS"
BASE COUNT          325 a          728 c          611 g          339 t
ORIGIN      
        1 gcctgctgga ggagtgcggc tgcaccgtca tcgccatccc ctgcaacacc tcccactact
       61 tcgtggaccg catccagcag gagatcggca tccccatcct ccacatgatc cgggagaccg
      121 cccggacctt ggtggcccag gggaagcgcc gccccgccat ccttgccacc gacggcacca
      181 tccgcaccgg cctgtatcag aaggagtgcg ccgccttcgg cctggaggcc gcccctccgg
      241 agcccgctgt ccagcgtctg gtcatgtcca tcatctacga ggagatcaaa cggggggagc
      301 ggggcagccg ggagaaattt gcccagatcg accgcgctct ggcctccatg ggctgtgact
      361 gcgccattct gggctgcacc gagctgtccg tcttccgcag ctaccacagc ctgcccccct
      421 tctacgtgga cgccatggag gtgctggccg agctggccgt cgtccgctgc ggcaaacagc
      481 tgcgcaccat ctaaggaaac atctccgggg cggtgcctgc ccacgcccgc cgttggaacg
      541 tactccgcaa attgaaaatg ggggtggcgc ccccatccac acatgacaac accaggaggt
      601 ttgctctatg gatctcacgc cccacaccct gggcgaatac tgcgccctgc tggagcccca
      661 gctggccgct cctctgcccg aggggctgga cctgtcccag tccgtcgccc tggtctcctg
      721 cgacagccag gaggtggtcc ccggcactct ctttctgtgc aagggcgccc acttcaagga
      781 ggcctatctg tcccaggccg cccagcgggg cgcggtggcc tatgtgagcc agacccccta
      841 ccctgccgtg gatctgccct gcatcctggt gtccgacatg cgccaggtga tggcccccct
      901 ggccgaccgg tactacggcc atccctccgg gctcctgtcg gtcatcggca tcaccggcac
      961 caagggcaag tcctccaccg cctattatct gaagtatatc ctggatgagt atctggcgga
     1021 gcagggcggg ccggagagcg gcatcatctc ctccatcgac acctacgacg gctcggagcg
     1081 cttcgagtcc cacctcacca cgccggagcc cctggatctc cagcgtcact ttgcccacgc
     1141 ggtggcggcc ggcatcccct acctcaccat ggaggtgtcc agccaggcgc tgaagtacca
     1201 ccgcaccctg tgcacccgct tcgccgccgc ctgcttcctg aacatcggcc tggaccacat
     1261 ctcccccatc gagcaccccg actttgagga ctacttctcc tccaagctgc ggatcttcgc
     1321 ccaggcggag gtggggtgcg tcaatctgga ctgcgaacac gccggccggg tgctggccgc
     1381 cgcccaggcg ggcagccgcc aggtcatcac cttctcccag aaggacccgg aggcggacat
     1441 ctatgcctcc caggtacgca agcggggcaa cgacatcctg ttccgggtgc gttcccgccg
     1501 cttttcccgg gagttccggc tgaccatgcc cggcctgttc aacgtggaga acgccctggc
     1561 cgccatcgcg gtgtgcgagg ggctgaacat cccggagcgg gccgtctatg tggggctgat
     1621 gaaggcccgg gtccccggcc gcatggaggt gtacaccaat gcggacagcc acatcgtggc
     1681 catcgtggac tacgcccaca accgcatgtc cttcgagacc ctgttccgct ccgttcaaac
     1741 ggagtacccc ggccgccggg tggtcaccgt gttcggctgt ccggggaaaa aggccctgga
     1801 ccgccggcgg gacctggggg agatctccgg tcgctgctcc gacctggtgg tgctgacaga
     1861 ggaggactcc ggggaggagg acaccctctc catctgccgg gaaatcgcct cctatgtgga
     1921 ggcccagggc tgcgactact ccatcgagcc caaccggggg gaggccatcc gccaggcggt
     1981 gctgggctgc cgggagccct ccg
//
LOCUS       DBMDSJ010000334         1997 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12786_length_1997_cov_1.593354, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000334 DBMDSJ010000000
VERSION     DBMDSJ010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12786_length_1997_cov_1.593354"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(149..412)
                     /gene="rpsT"
                     /locus_tag="ACZPAQ_07805"
     CDS             complement(149..412)
                     /gene="rpsT"
                     /locus_tag="ACZPAQ_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR0903932.1"
                     /translation="MPNIKSAKKRVLVNQAKAMQNKAAKSALKTDIKKFEAAVAEGNR
                     SEADVAYKVAVKAVDKAAAKGLLHKNNAANKKSSLTLKLNKLA"
     gene            611..1501
                     /gene="gpr"
                     /locus_tag="ACZPAQ_07810"
     CDS             611..1501
                     /gene="gpr"
                     /locus_tag="ACZPAQ_07810"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HIR0903933.1"
                     /translation="MRNRRTDLALEARELWQESAEERTQLEGVEAREEMREGIPVTRV
                     SILDRRGEEALGKPVGTYVTLTLDGLARREENAFGRAARAVAAELGELLGFLHPEAPV
                     LVVGLGNRAITPDAVGPKVHEYTLVTRHLVRQVPEHFGDLRPVASLSAGVLGTTGMES
                     GELVRAVSERLRPACVIAVDALASRSLKRVCRTVQLADTGIAPGSGVGNHRAALDRAS
                     LGVPVIAVGVPTVVDGATLAADLLGTEELPPLGEGRDLMVTPKDIDSQVADLSKVIGY
                     GIDLALQPSLTLEDLDLLLS"
BASE COUNT          385 a          548 c          674 g          390 t
ORIGIN      
        1 atttctggtc cataagggat tcttcatatc gtgatatcac ctcctgtttt ttagtataca
       61 ggaagaatga aagaaagaaa ataagggaaa attgagaaaa gccgcctgca aagcagacgg
      121 cttttatggt gcaaacacag ggcaggactc aggccagctt gttcagcttc agagtcaggc
      181 tgctcttctt gttcgcggca ttgttcttgt gcagcagacc cttggcagca gccttgtcga
      241 ccgccttgac agccaccttg taagcgacat cggcctcgct gcggttgcct tccgccaccg
      301 cggcttcaaa cttcttgatg tcggtcttca gcgcggactt agcggccttg ttctgcatgg
      361 ccttggcctg attgaccaga acacgctttt tggcagactt aatattcggc aaaccaaaca
      421 cctcctccga tcgaatcgta aaatggtgca cagaaactct gtgcagatat gtattgtacc
      481 attggatctt caaaaaatca actgtttttt taaatttttc ccgctttttt ggatagaatc
      541 cctggcgccg caaaaaatag ggcagaagcg gcagctattc cactatatct tgcggaggga
      601 ggagcgggac atgcggaaca gaaggacgga cctggctctg gaggccaggg agctgtggca
      661 ggagtcggcg gaggagcgta cccagctgga gggggtggag gcccgggagg agatgaggga
      721 gggcatcccc gtgacccggg tcagcatcct ggacaggcgg ggggaggagg ccctggggaa
      781 gcctgtgggg acttatgtga ccctgaccct ggacgggctg gccagacggg aagagaatgc
      841 tttcggccgg gcggcccggg cggtagcggc agagctgggg gagctgctgg gcttccttca
      901 tccagaggcc ccggtgctgg tggtggggct gggcaaccga gccatcaccc cggacgctgt
      961 gggccccaag gtccacgagt acaccctggt gacccggcac ctggtacggc aggtgccgga
     1021 gcacttcggg gacctgagac cagtggcatc cctgtctgcc ggcgtgctgg ggaccaccgg
     1081 tatggagagc ggggaactgg tccgggcagt gtcggagcgg ctgcggcccg cctgcgtgat
     1141 cgcggtggac gcgctggcct cccggtccct aaaacgggtg tgccgcacgg tgcagctggc
     1201 ggataccggc atcgccccgg gctctggggt gggcaaccac cgggcggcgc tggaccgggc
     1261 ttccctgggg gtgccggtga ttgcggtggg ggtgcccacc gtggtggacg gggccaccct
     1321 ggccgccgat ctgctgggta cggaggagct gcctcccctg ggggagggga gggacttgat
     1381 ggtcacgccc aaggatatcg acagccaggt ggcggacctg tccaaggtga tcgggtacgg
     1441 cattgacctg gctttacagc cgagcctcac cctggaagac ctggatctgc tgttgagctg
     1501 aggggggagc cgctgccaaa tgggaagaaa gggaggtccc gacagagggg cggctcccag
     1561 agccgccctt caggccttcc ccccaccggg gggaaggtgg cgcggcgtat gccgcgacgg
     1621 atgaggggtc tttcttttcc cgtctcagtc atgatttgtc agagggattc caccctgcgg
     1681 ggcggaatcc cttttgtggc atcctgagac ggaggcgggc ctgtactcct tatttctaat
     1741 ggtttcttcc cgaaacgccc ctgccgtgga ccttccccac ataggaccaa aacaccccca
     1801 gccaggaccg gaacagagcc ggtcctggct gggggctttt taagaagaaa tttccccggg
     1861 gatgaccaca gtctcccggt agtactgggc ctggaggaac tgctggtaga accgggcctc
     1921 ctccgggacg agagcatctg tgaggatgcc gccgctcagg aagagccccc gagtatggat
     1981 caagggggag atggcct
//
LOCUS       DBMDSJ010000335         1997 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12792_length_1997_cov_0.973101, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000335 DBMDSJ010000000
VERSION     DBMDSJ010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12792_length_1997_cov_0.973101"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(125..988)
                     /locus_tag="ACZPAQ_07815"
     CDS             complement(125..988)
                     /locus_tag="ACZPAQ_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR0903934.1"
                     /translation="MSAKFGPAGNSDSFSKAHRSSVEAPEWIAGLGLDCYEYQCGKGV
                     HVGEETARRVGERARQAGISLSLHAPYFINLANPDRSALEKTIGYISAACLVASQMGA
                     GRVVIHSGALMKRSRGEALDIARRSLREVIAACDDQGFGSIALCPETMGKINQLGDLD
                     EVLELCTLDKRLIPCIDWGHLYARSLGADDGQEAFSRMLDRVEEVLGTERAAQFHSHF
                     SHIQFTPSGGEKCHRTFADNDGYGPDWAPLAAQVARRGWSPTFICESAGTQAEDALTM
                     KRIYEQYAANP"
     gene            complement(985..1491)
                     /locus_tag="ACZPAQ_07820"
     CDS             complement(985..1491)
                     /locus_tag="ACZPAQ_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR0903935.1"
                     /translation="MPFSPIPRGVYPSVTHISPSELAAKGVRLVLADLDNTLVPYHVT
                     EPSPEVAAWAAELAEQGIQLFLLSNSRKPGRAQKFAEQLGIPYQGHSGKPKKGGYLRA
                     MERMGYRPEETVMVGDQIFTDTLGANNAGVIPLLVEPIRLAGNPGRYLRYAAETPFRM
                     LGRRRPFL"
     gene            1629..>1997
                     /locus_tag="ACZPAQ_07825"
     CDS             1629..>1997
                     /locus_tag="ACZPAQ_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903936.1"
                     /translation="MTRDTIIITTPPQTLRSVQGWDVTPGHLAYRVGRGPHLFRAGGG
                     TVQPRGGIMVVDDQGFDGLGDPGPLCQEVVRECSARGFTGAVLDFDAKLPPLERMAAT
                     LEEGFARRGWTLYVPESYGAR"
BASE COUNT          346 a          622 c          642 g          387 t
ORIGIN      
        1 aaaaagctgc agaataccgg tgtcccggcc ctccagccac atagaaaacc agcggcggac
       61 agcctgctcc cgtcctgcca tatccatacc gtttctcctt tcggcgcagc tccatgagag
      121 cctctcatgg gtttgcggca tactgctcgt agattcgttt catagtcaga gcatcctccg
      181 cctgagtccc cgcgctctcg cagataaaag tggggctcca gccccgtcgg gccacctgag
      241 cggccagggg cgcccagtcg gggccgtagc cgtcattgtc ggcaaaggta cggtggcact
      301 tctcgccccc gctgggagta aactggatgt gagagaagtg gctgtggaac tgggcagccc
      361 gctctgtccc cagtacctcc tccacccgat ccagcatccg ggaaaatgcc tcctgtccgt
      421 cgtctgcccc cagggagcgg gcgtacagat gtccccagtc gatgcagggg atcagccgct
      481 tatccagggt acacagctcc agcacctcgt ccagatctcc cagttggttg atctttccca
      541 tcgtttcggg gcacagggcg atagagccga agccttggtc atcacaggcg gcgatcacct
      601 cccgcaggga tcggcgtgcg atgtccagcg cttctccccg gctgcgcttc atcagcgccc
      661 cggagtggat gaccacccgg ccggccccca tttggctggc caccagacag gcggcagaga
      721 tatagccgat ggtcttttct agggccgacc ggtcgggatt ggccaggttg atgaagtagg
      781 gggcatggag ggacagggag atgccagcct gacgggcccg ctcccccacc ctgcgggcgg
      841 tttcctctcc cacatggacc cctttgccgc actggtattc atagcagtcc agccccaggc
      901 cggcgatcca ttccggggcc tctaccgagg agcggtgtgc cttggaaaag ctgtcggaat
      961 tgcccgccgg gccaaatttg gcgctcatag gaagggcctc cttctgccca gcatccggaa
     1021 aggggtttca gcggcgtagc ggaggtatcg gcccggattg cccgccagcc gaataggctc
     1081 caccagcagg gggatgaccc cggcgttgtt ggccccaagg gtatcggtaa agatctggtc
     1141 ccccaccatc accgtttcct ccggccggta gcccatccgc tccatagccc ggagatagcc
     1201 cccttttttc ggtttgcctg agtgcccctg ataggggatg cccagctgtt cggcaaactt
     1261 ctgcgcccgg ccgggctttc ggctgttgga cagcaggaac agttggatcc cctgctccgc
     1321 cagctctgcg gcccaggcgg ccacctcggg ggaaggctct gtcacatggt aggggacaag
     1381 ggtgttgtcc aggtctgcca gtaccagccg gacccctttg gcggccagct cgctggggct
     1441 gatgtgggtg accgagggat ataccccccg gggaatgggt gaaaagggca cgggtaggtc
     1501 ctccttatgt ctcgttctat gctttggccc gctggcatag agtggaggga gcgggcctcc
     1561 atttggtctg accacaatca tcggttaagt atatcacgat acaatattct gggcaagggg
     1621 gagccgtcat gacgagggac accattatca ttaccacgcc gcctcagact ctgcggtcag
     1681 tacaggggtg ggatgtgacc ccaggtcacc tggcctatcg ggtggggagg gggccgcatt
     1741 tgttccgggc gggcggcggc acagtccagc ccaggggcgg gattatggtg gtggacgatc
     1801 agggcttcga cggtctgggc gaccccggcc cgctgtgcca ggaggtggtc cgggagtgtt
     1861 ccgcccgtgg cttcacgggg gcggtactgg actttgatgc caagctgccc ccgctagagc
     1921 ggatggccgc cacactggag gagggatttg cccgccgggg atggaccctc tatgtgccgg
     1981 aaagttacgg agccagg
//
LOCUS       DBMDSJ010000336         1983 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12899_length_1983_cov_1.197662, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000336 DBMDSJ010000000
VERSION     DBMDSJ010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12899_length_1983_cov_1.197662"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="ACZPAQ_07830"
     CDS             complement(<1..421)
                     /locus_tag="ACZPAQ_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903937.1"
                     /translation="MESFHCFSSEPFSPDAAQSRASSGQTPVIPLPDPGEGGPVAPDI
                     GGTPVIPLPNPGEGGPVAPGGGIGMVPGWPRWSSIRFLNAARSYPPFRIFIGSTLAVR
                     SLEFTAVSSYRRMASGYRSVTVSGQDGYIYLQKTLPDT"
     gene            complement(643..768)
                     /locus_tag="ACZPAQ_07835"
     CDS             complement(643..768)
                     /locus_tag="ACZPAQ_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903938.1"
                     /translation="MPKNSQQDQKQARQKKNSKGAQAQGTQCRDRDQRQSSDPAM"
     gene            complement(844..>1983)
                     /locus_tag="ACZPAQ_07840"
     CDS             complement(844..>1983)
                     /locus_tag="ACZPAQ_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014124.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HIR0903939.1"
                     /translation="LFHALHQALRYEDGLIPLIFFDEFDSELGGVSRGWLKYFLAPMQ
                     DGEYTLDGSPLPIPGAVFVFAGATASSFSEFLPQAPEDVHRFQAIKGPDFVSRLKGIL
                     DIKGPNPTCVTDKKHIIRRAMLLRSLILKNVKGICHPDTGRVDISRGLLCALLRVSEY
                     RHGARSIEFILGMSRLAGASRYTPSCLPLAAQLDIHVDVQDFMRKLSFEQMMGEAVEQ
                     YAFTAHEKYRQKHLADAQARGATPKELSLVRQEPEMADWSDLDEFYKEGHRSQIRYLG
                     ERLESFNMSIGLRPVLPGAADTIMDLYGPVLEQLSELEHERWMKDKRADGWRYGKRDS
                     ELKLHPDMRPYQELDESTKEFIRMSVRNVPDYLKEIGYELYRKSF"
BASE COUNT          378 a          571 c          682 g          352 t
ORIGIN      
        1 ccgtgtcggg cagggtcttt tgcaggtaga tgtagccgtc ctggcccgac acggtgacgg
       61 accggtatcc ggaagccatc cggcggtaag aggagactgc ggtgaactcc agagagcgaa
      121 ccgccagcgt gctgccgatg aagatgcgga aggggggata ggagcgggcc gcgttgagaa
      181 agcggatgct gctccaacgg ggccagccgg ggaccatgcc gatgccgcct cctggggcca
      241 cgggaccgcc ctctcccgga tttgggagag ggatcaccgg agtacccccg atgtcgggcg
      301 ccacaggacc gccctctccg gggtcaggca gggggatcac aggggtctgg ccgctggagg
      361 cacgggactg ggcggcgtcg ggggagaagg gttcggaaga gaagcagtgg aaggattcca
      421 tatgaagagc cgaacaagct cggctttcct cctttcggtt tgggctgccc tatcctattc
      481 cggggacggg agaggggtga caggcgtgac cgcacccttt tttcgggaaa ggggagggga
      541 agctggccga aaggagcaca ggccggacct ggagggaaag gataccagga gaggcgctga
      601 aaagcggaaa ccggcggctg cctatgggca gccgccggtt tttcacatcg cggggtcgga
      661 ggactggcgc tgatcccggt cccggcactg cgtgccctgg gcctgggcgc ccttgctgtt
      721 cttcttctgc cgggcctgct tttggtcctg ctggctgttc ttaggcataa aatctcacct
      781 cagcagtagt attgcctggg aaaagagatc cataccagaa aaacagccga actgcaaaaa
      841 aactcagaag gacttccggt acagctcgta gccgatctct ttcaggtagt cggggacgtt
      901 gcggacggac atccggatga actccttggt ggactcgtcc agctcctgat agggccgcat
      961 gtctgggtgg agtttcagct cgctgtcccg cttgccgtag cgccagccgt cagcccgctt
     1021 gtccttcatc cagcgctcgt gctccagctc agagagctgt tccaacactg ggccgtagag
     1081 atccatgatg gtgtccgcgg ccccgggaag gacggggcgc aggccgatgc tcatgttgaa
     1141 agactccagc cgctccccca gatagcggat ctgggagcgg tggccctcct tgtagaactc
     1201 gtccaggtcg ctccagtccg ccatctccgg ttcctgccgc accagggaga gctccttggg
     1261 ggtggcaccc cgggcctggg cgtcggccag gtgcttctgg cggtacttct cgtgggcagt
     1321 gaaggcgtac tgttccaccg cctcccccat catctgctca aaggacagct tgcgcataaa
     1381 gtcctggaca tccacatgga tgtccagctg ggcagccagg ggaaggcagg agggggtgta
     1441 ccggcttgcg ccagccagcc ggctcatacc caggataaat tcaatggacc gggccccatg
     1501 gcggtactcc gacaccctca gcagagcgca cagcaggccc cgggagatgt ccacccgtcc
     1561 ggtatccggg tggcagatac ccttcacgtt tttcaggatc aggctgcgca gcagcatagc
     1621 acggcggatg atgtgctttt tgtcggtaac gcaggtgggg ttggggccct tgatgtccag
     1681 gatgcctttg aggcggctga cgaaatcggg gcccttgatg gcctggaagc ggtgcacgtc
     1741 ctccggggcc tggggaagaa attccgagaa ggaggaggcg gtggcacccg caaagacaaa
     1801 gaccgccccc gggatgggca gggggctgcc gtccagggtg tattccccgt cctgcatggg
     1861 ggccaggaaa tacttcagcc agccccggga gaccccgccc agctcggagt caaactcgtc
     1921 gaagaagatc agggggatca gcccgtcttc atagcgcaga gcctgatgga gggcgtggaa
     1981 gag
//
LOCUS       DBMDSJ010000337         1977 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12941_length_1977_cov_1.524520, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000337 DBMDSJ010000000
VERSION     DBMDSJ010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12941_length_1977_cov_1.524520"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="ACZPAQ_07845"
     CDS             complement(<1..191)
                     /locus_tag="ACZPAQ_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903940.1"
                     /translation="MSMSEKKKKRLLTGIIVLTVLVMGLITWFVGKPLIRFIENPDGF
                     RAWVDGHGIWGRVLYVGMV"
     gene            complement(260..>1977)
                     /locus_tag="ACZPAQ_07850"
     CDS             complement(260..>1977)
                     /locus_tag="ACZPAQ_07850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0903941.1"
                     /translation="HWQLYPQYYMGQSNYRYEIRDGAGNLLKSTYNGEGCLARLESVQ
                     TVSEILVDSPPYGDRLEETESSGIYWKAVDLTYTVTGYVLQDLAYDDQFSQYVSLVGN
                     MYQYRFALVVLVCVCLAALLCLVVYLLSAAGHRPDRQGIALNPFDKLPGDLYLAAVIG
                     GVTLLAMLFRRSFYWEGWITLVTGIACLLAAGILVTAFLMSLATRVKYGQGYWWRRSV
                     IGFCLVLLLRACRFVVRGARSLFGMLPTIWKWLLIGGLAWIFTLACLNPYTTGMGALA
                     TACLWAFLIVYPAWSFGQLRKAARRMAEGKFQEKVPLDHLHGSFRQIAQDLNALGQGA
                     SLAVERQLRSERMKTELITNVSHDIKTPLTSIVNYVDLLQKPHTPQEGEQYLEVLARQ
                     SQRLKKLTEDLVEMSKASSGNIPVNLAPVNVVELVNQALAEYEEKLKKAQLPVVFTAR
                     QSEILTMADGRLFWRVLDNLLGNCVKYAQPGTRVYVDVVKYEKKVMISMKNVSRAELN
                     LPAEELMERFVRGDCARNTEGSGLGLNIAKTLMELQKGKLNLVIDGDLFKAVLLFDNL
                     EPGQS"
BASE COUNT          389 a          654 c          575 g          359 t
ORIGIN      
        1 atcaccatgc ccacatacag aacccgtccc cagattccgt ggccgtccac ccaggcccgg
       61 aagccgtcgg ggttctcgat gaaccggatc aggggcttgc ccacaaacca ggtgatgagc
      121 cccatcacca aaaccgtcag cacaatgatg cccgtcagca ggcgtttttt ctttttttcc
      181 gacatagaca taagccagaa ctcacgattc ctttctccca atccccggga attgcagcgg
      241 cggatcccct ccggggcgct cagctctgcc cgggctccag gttgtcgaag aggagaaccg
      301 ccttaaacag gtccccgtcg atgaccaggt tcagctttcc cttttgcagc tccatcagcg
      361 tcttggcgat gttgaggccc aagccgctgc cctcggtgtt ccgggcgcag tcgccccgga
      421 cgaaccgctc catgagctcc tccgccggca gattcagctc cgcccgggag acgttcttca
      481 tggaaatcat aacctttttt tcgtatttta caacatctac atacactctt gtccccggct
      541 gggcgtactt gacgcagttg cccaggagat tgtccagcac ccgccagaag agcctgccgt
      601 cggccatggt gagaatctcc gactgccggg cggtgaagac caccggcagc tgcgcttttt
      661 tgagcttctc ctcatactcc gccagggcct gattcaccag ctccaccacg ttcaccgggg
      721 ccagattcac cggaatgttg ccggaggagg ccttggacat ctccaccaaa tcctcggtga
      781 gcttcttcag ccgctgggac tgccgggcca gcacctccag gtattgctcc ccctcctggg
      841 gggtatgggg cttttgcagc aggtccacgt agttcacaat ggaggtcagg ggcgttttga
      901 tgtcgtggga gacgttggtg atgagctcgg tcttcatccg ctccgaccgc agctgccgct
      961 ccacggccag gctggccccc tggcccaggg cgtttaaatc ctgggcaatc tggcggaagg
     1021 agccgtggag atggtccagg ggcaccttct cctgaaattt cccctcggcc atccgccggg
     1081 cggccttgcg gagctggccg aaggaccagg cgggatagac gatgagaaac gcccacaggc
     1141 acgccgtcgc cagggccccc atccccgtgg tgtagggatt caggcaggcc agggtgaaga
     1201 tccaggccaa gccgccgatc agcagccact tccagatggt gggcagcatg ccgaacagag
     1261 agcgggcgcc ccggaccaca aaccggcagg cccgcagcag cagcaccagg caaaagccga
     1321 tgaccgagcg ccgccaccag tagccctgcc cgtatttcac ccgggtggcc agggacatga
     1381 ggaaggccgt caccagaatc cccgccgcca gcaggcaggc gatgccggtc accagggtga
     1441 tccagccctc ccagtagaag gaccggcgga acagcatcgc cagcagcgtc acgccgccga
     1501 tcaccgcagc caggtacaaa tccccgggga gcttgtcaaa gggattgagg gcgatgccct
     1561 gccggtccgg ccggtgtccg gcggcgctga gcagatacac caccaggcac agcagggccg
     1621 ccagacagac gcacaccaaa accaccagag caaagcggta ctggtacatg ttgcccacca
     1681 ggctcacata ttgggaaaac tggtcgtcat aggccagatc ctgcagcacg tagccggtga
     1741 cggtataggt caggtccacg gctttccaat atatgccgct ggactccgtc tcctccagcc
     1801 ggtcgccgta gggcgggctg tccaccagga tttcgcttac ggtctggacg gactccagcc
     1861 gggccaggca cccttcgccg ttgtaggtgc tcttcaagag gttgcccgcc ccgtcccgga
     1921 tttcatagcg gtaattgctc tgtcccatgt aatactgggg gtacagctgc cagtggc
//
LOCUS       DBMDSJ010000338         1974 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12965_length_1974_cov_1.450080, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000338 DBMDSJ010000000
VERSION     DBMDSJ010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12965_length_1974_cov_1.450080"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="ACZPAQ_07855"
     CDS             complement(<1..555)
                     /locus_tag="ACZPAQ_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HIR0903942.1"
                     /translation="MDAILQTLARELERSPEHVQNVVTLIDEGATIPFIARYRKELHG
                     SMDDQLLRRLADRLQYLRNLEARKGEVAAAIENQGKLTQELSDAIAAAATLAEVEDLY
                     RPYKQKRRTRATIAREKGLEPLVRLLSAQGPAVDPRQAALDFLDPDKGVETAEDALQG
                     ASDILAEEISDDAYIRRELRQLYLR"
     gene            complement(628..1125)
                     /locus_tag="ACZPAQ_07860"
     CDS             complement(628..1125)
                     /locus_tag="ACZPAQ_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903943.1"
                     /translation="MKKFKPTKFDVMVLGLTALFLLVTGLYFVVCNGAFCPLCQDYFI
                     SNGETYIWDTRGGYLEPLSVYRHCHSDGVSMLDIRTGMHSECSSTARTGIVRFADDPG
                     PHPLYCLRHRPDQSLGRFQMLDQNQDYTVVWGLREGEAVQAEDCFLTLTWDGEWDQWE
                     IHITW"
     gene            1804..>1974
                     /locus_tag="ACZPAQ_07865"
     CDS             1804..>1974
                     /locus_tag="ACZPAQ_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903944.1"
                     /translation="MNSAYHRLYRTLGINIAYYRNMKDMSQEQLGDRLEIDQSHVSKI
                     ERASIGISMDMLC"
BASE COUNT          380 a          583 c          622 g          389 t
ORIGIN      
        1 gcgcagatac agctgccgca gctccctgcg gatgtaggcg tcgtcggaga tctcctcggc
       61 caggatgtca ctggccccct ggagggcgtc ctccgccgtc tccaccccct tgtccgggtc
      121 cagaaagtcc agggccgcct gacgggggtc caccgccggg ccctgagcgg agagcagccg
      181 gaccaggggt tccagcccct tctcccgggc gatggtggcc cgggtgcgcc gcttctgctt
      241 ataggggcgg tacaggtcct ccacctccgc cagagtggcg gccgcagcga tggcgtcgga
      301 gagctcctgg gtgagcttgc cctggttctc gatagcggcg gcgacctccc ccttccgggc
      361 ctccaggttc cgcaggtact gaaggcggtc cgccagccgg cgcagcagct ggtcgtccat
      421 ggagccgtgg agctccttgc ggtagcgggc gataaaaggg atggtggcac cctcgtcgat
      481 gagggtcacc acattttgta catgctccgg agagcgttcc agctcccggg cgagcgtttg
      541 aaggatggcg tccatgcaaa agtctccttg gttcagaaat ccctcccagt gtaaccgaat
      601 tggaagaaaa agtccagggg gaaagggtca ccaggtgata tggatctccc actggtccca
      661 ctccccgtcc caggtgagag tgagaaaaca gtcctccgcc tgaacggcct ccccttcccg
      721 gagcccccag acgactgtgt agtcctggtt ctggtccagc atctggaagc ggcccagaga
      781 ctggtccggc ctgtggcgga ggcagtagag aggatggggg cccgggtcat ccgcgaagcg
      841 gacgatgccc gtccgggcgg tggaggagca ctcgctgtgc atgccggtcc tgatgtccag
      901 catggagacg ccgtcggagt gacagtggcg gtagacggac aggggctcca gatagccgcc
      961 cctggtgtcc cagatgtagg tctcgccgtt gctgatgaag tagtcctggc acagggggca
     1021 gaaggcaccg ttgcagacga cgaagtacag gccggtgacc agaaggaaaa gcgcagtcag
     1081 ccccaggacc ataacgtcga atttggtggg cttgaatttt ttcatccgga ttcctccctc
     1141 aaagacaaca gttttctgta aagcacgccc tcctgcagag cccggtgccc ctgtcgggcg
     1201 gcgtgccgtt ttccccaaag ccttccccct ctggggggaa ggtgtcacgg cgcatgccgt
     1261 gacggatgag gggtcttcct tcccgtgtcc cagccgtggg atggcgaaag gggttccgcc
     1321 ctgcggggcg ggtccctttc ccagcgatgg gaaaggaacc aaaggatcgc cgggggacac
     1381 tcccggcggg accacggctc cacgtgcgtt ccgcctgggt cccacagtcc gctttccccc
     1441 ggccccccag gcacgtgagc caccgagcgg agttgtctga aacctgaccg gcgcggcaat
     1501 gctttcgcac tgaccagcgc cttgcgccgc tgccgctgat gccctgcgtc atttgggtcc
     1561 atgcgctgtg ccagctttgc gcctagtgtt tccggcctgg caggggttgc ggccccctgt
     1621 tcgcgttgct gccgcgagtg cgttcaatgg gagcggccca acgataatct tgcacatttg
     1681 ctcaagatac atgatataag agggagggct gtccaggaag atattcacaa tgcatatata
     1741 tgcagtttga atatctggta aaaaatctca gtcaatttat aatgatatgc agaggtttag
     1801 cttatgaact ctgcatatca ccgcctatac agaacattag gcatcaatat tgcctattat
     1861 cgaaatatga aagatatgag tcaggaacag ctcggagaca ggttggagat cgaccagagc
     1921 catgtcagca aaatcgagag ggcttccatc gggatatcca tggatatgct gtgt
//
LOCUS       DBMDSJ010000339         1969 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12992_length_1969_cov_1.374732, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000339 DBMDSJ010000000
VERSION     DBMDSJ010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12992_length_1969_cov_1.374732"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(155..355)
                     /locus_tag="ACZPAQ_07870"
     CDS             complement(155..355)
                     /locus_tag="ACZPAQ_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0903945.1"
                     /translation="MSLRTKIRELRLRDRLSQEELAQRVGVRRETIGHLENGHYNPSL
                     KLAMDIAHVFGCTVEDIFFFED"
     gene            complement(352..705)
                     /locus_tag="ACZPAQ_07875"
     CDS             complement(352..705)
                     /locus_tag="ACZPAQ_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903946.1"
                     /translation="MRHPFNYLSLLSLPALAALIYFPTGDADFLGFLGFLVFLRYLWV
                     NPDELFLLTLRRAATSAFLAECVLLAPLLLLFYLLEPGSSPMPQALGLSFALSLIFFC
                     LFHTWLEWKESRDCA"
     gene            873..1778
                     /locus_tag="ACZPAQ_07880"
     CDS             873..1778
                     /locus_tag="ACZPAQ_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HIR0903947.1"
                     /translation="MTQRESGVEQAARVLPLRLRSGVMSLPEDRRSQIEELRLRVGLP
                     MAAVLPEGERALAGPPVTQQELEQLLELASRASVHAVLDQLSRGYLTVQGGHRVGLCG
                     TVALEDGAVRTIRKLSSAAVRVARQFPGAAEEVLPSLLEGGRLESALILAPPGLGKTT
                     LLRDLVRAVSAGEGAPPRRVGVADERGEIAALFDGVPQLDVGPRTDVMEGCPKAWGLM
                     TLLRGMNPQVLAVDEITAPEDVAALVSAAGCGVTLLATAHGAGREDLSRRKLYRGLLE
                     EGVFRRLVRIRREAGRRIYEVEVLE"
     gene            1775..>1969
                     /locus_tag="ACZPAQ_07885"
     CDS             1775..>1969
                     /locus_tag="ACZPAQ_07885"
                     /inference="COORDINATES: protein motif:HMM:NF021090.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HIR0903948.1"
                     /translation="MIRMLGAVCLAAGPVWLGMSAAAELAHRERALGALCAGLELMER
                     ELALRLTPLPQLMEELAHRTE"
BASE COUNT          356 a          548 c          772 g          293 t
ORIGIN      
        1 aacttcgcca cacccggaaa gccgcggtcc gaagtgaact caccggacag cttttgtata
       61 ttaccacacc tgtcaaaata atgcaagccc tttttgaaaa aaacttgaaa aatttttggc
      121 gggcgtctcc gggggcggga gagggccgat tgggtcagtc ctcaaagaag aagatatcct
      181 ccacagtgca cccaaagaca tgggcgatgt ccatggccag cttcagggag gggttgtagt
      241 gtccattttc caggtggccg atggtctccc gccgcacgcc cacccgctgg gccagctcct
      301 cctggctgag ccggtcccgg aggcggagct cccggatctt ggtgcgcagg ctcatgcgca
      361 gtccctgctt tccttccact ccagccaggt gtggaacagg cagaagaaga tcagggagag
      421 ggcgaagctg aggcccaggg cctggggcat gggactggag ccgggctcca ggaggtaaaa
      481 gaggagcagc agcggagcca ggagcacgca ctcagccagg aaggcgctgg tggccgcccg
      541 gcgcagggtg agcaggaaga gctcgtccgg gttgacccac aggtagcgca gaaagaccaa
      601 aaagccgaga aagccgagaa aatccgcgtc cccggtgggg aaatagatca gggcggccag
      661 ggcggggagt gagagcagag acagatagtt gaacgggtgc cgcatagaga ggctcctttc
      721 tgagatgaga gatatttctc acatgttgtg agtgaaatat atcacatccc tggagagatg
      781 tcaagagaga tgtgcgaaaa atatcacaca aaaaggagga ataggcgggg gcctgtcctc
      841 ataggatgag tcaaacggag gtggacaggc tgatgacgca gagagaatcc ggcgtggagc
      901 aggcggcgcg ggtcctgccc ctgcgcctgc ggagcggggt gatgtccctg ccggaggacc
      961 ggcggagcca gatcgaggag ctgcggctgc gggtggggct ccctatggcg gcagtgctgc
     1021 cggaggggga gagggcactg gccgggcccc ccgtgaccca gcaggagctg gagcagctgc
     1081 tggaactggc cagccgggcc tctgtccacg ccgtgctgga ccagctcagc cgggggtacc
     1141 tgaccgtcca ggggggacac cgggtggggc tgtgcggcac ggtggccctg gaggacgggg
     1201 cagtgcggac catccggaag ctgtcctccg ctgcggtgcg ggtggcgcgg cagttccccg
     1261 gggcggcgga ggaggtgctg ccgtcgctgt tagagggcgg gcggctggag agcgccctga
     1321 tcctggcccc tccggggctg ggcaagacca ccctcctgcg ggatctggtg cgggccgtct
     1381 ccgccgggga gggggcgcct ccccggcggg tgggggtggc cgacgagcgg ggggagatcg
     1441 cggcgctgtt cgacggggtc ccccagctgg acgtgggccc ccgcaccgat gtgatggagg
     1501 ggtgtcccaa ggcgtggggg ctgatgaccc tgctccgggg gatgaacccc caggtgctgg
     1561 cggtggacga gatcaccgcg ccggaggacg tggcggcgct ggtgtccgcg gcgggctgcg
     1621 gcgtgacact gctggccacc gcccacgggg cgggacggga ggacctgtcc cggcggaagc
     1681 tgtaccgggg gctgctggag gagggagtct tccggcggct ggtccgcatc cgcagggagg
     1741 cgggccggag gatctatgaa gtggaggtgc tggaatgatc cggatgcttg gcgcggtctg
     1801 cctggcggcg ggcccggtct ggctgggaat gagcgctgcg gcggagctgg cccaccggga
     1861 gcgggccctg ggggccctgt gcgccggact ggagctgatg gagcgggagc tggccctgcg
     1921 gctgaccccg ctgccccagc tgatggagga gctggctcac cggacggag
//
LOCUS       DBMDSJ010000340         1968 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_12999_length_1968_cov_1.720407, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000340 DBMDSJ010000000
VERSION     DBMDSJ010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12999_length_1968_cov_1.720407"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="ACZPAQ_07890"
     CDS             <1..976
                     /locus_tag="ACZPAQ_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HIR0903949.1"
                     /translation="ITCALFGALKAGDVLLSAVGAPYDTLMGAIGTADKGHGSLRDYG
                     VEYRQVELKDDCPDLEGLARAAADPRVKAVLIQRSKGYSTRASLSVAEIGEMCRIVKE
                     VNPGTAVLVDNCYGEFVETQEPTHVGADLVVGSLIKNPGGGLAPTGGYIAGRRDLVEG
                     AAMRLTAPGIGGECGCTLGQNRLLYQGLFLAPHTVAQAVKTAAFAAGVMELLGYETEP
                     ASSAVRHDIIQMIHMREPEALKKFCKGIQFGAPVDSYVTPEPWDMPGYDCQVIMAAGA
                     FVQGASIELSADAPMRPPYTVYLQGGLTFESGRLGVLLAVQELLGGKD"
     gene            1026..1736
                     /gene="yunB"
                     /locus_tag="ACZPAQ_07895"
     CDS             1026..1736
                     /gene="yunB"
                     /locus_tag="ACZPAQ_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HIR0903950.1"
                     /translation="MDMRCRLRAVRWRLDPPLRGRAPRRGPGGGRGRGWLLTALVALA
                     LAAGIVGGLQHQLRPLVEAAARAQAANALTAVIDRAIAADLAERDVDYGDFVTIQRDA
                     SGAITAMTTDMAALNQLRAQLVAQILSALEGVDASEIRIPLGSLVDSELVWARGPELR
                     VRALRVGTVSAEFRSDFSQAGVNQTLHRIELEVAVPVTLILPGGQTELTIETGLRVAE
                     TVIVGAVPDTYLTLNGAA"
     gene            1758..>1968
                     /locus_tag="ACZPAQ_07900"
     CDS             1758..>1968
                     /locus_tag="ACZPAQ_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase LigA-related protein"
                     /protein_id="HIR0903951.1"
                     /translation="MDEKQELECLRRELVQANYEYYVQDAPTMTDYDYDHKLRRLEEL
                     EGAHPEWITPDSPTQRVGGKALDAFQ"
BASE COUNT          308 a          649 c          730 g          281 t
ORIGIN      
        1 catcacctgc gccctcttcg gggcgctgaa ggcgggggat gtgctcctgt ccgcggtggg
       61 ggccccctat gacaccctga tgggggccat cggcacggcg gacaagggcc acggctccct
      121 gcgggactac ggggtggaat accggcaggt agagctgaag gatgactgcc cggacctgga
      181 ggggctggcc cgggcggcgg cggacccccg ggtgaaggcg gtgctgatcc agcgatccaa
      241 ggggtactcc acccgtgcct ccctgtcggt ggcggagatc ggggagatgt gccgcatcgt
      301 aaaggaggtc aacccgggca ccgccgtcct ggtggacaac tgctacgggg agttcgtgga
      361 gacccaagag cccacccatg tgggggcgga cctggtggtg ggctccctca tcaagaaccc
      421 cggcggcgga ctggccccca cggggggata tatcgccggc cgccgggacc tggtggaggg
      481 ggcggccatg cggctgaccg caccgggcat cggcggggag tgcggctgca ccctggggca
      541 gaaccgcctg ctctaccagg ggctcttcct ggccccccac acggtggccc aggcggtgaa
      601 gaccgccgcc ttcgccgccg gcgtgatgga gctgctgggc tatgagaccg agcccgcctc
      661 ctccgccgtc cgccacgaca tcatccagat gatccatatg cgggagccgg aggcgctgaa
      721 aaagttctgc aagggcatcc agttcggcgc gccggtggac tcctatgtga cgccggagcc
      781 ctgggacatg ccgggctacg actgccaggt catcatggcc gcgggggcct tcgtccaggg
      841 ggcctccatc gagctgtccg ccgacgcccc catgcgcccc ccctacaccg tctatctcca
      901 gggcggcctc accttcgagt cggggcggct gggcgtgctg ctggcggtgc aggagctgct
      961 gggcgggaaa gattgacata ggccaagcgc cctgatgaat atcctgaccc atagggggtg
     1021 aggatatgga tatgcgatgc cgcctgcggg ccgtccgctg gcggctggac ccgcccctcc
     1081 ggggacgggc gccccggcgg ggaccggggg gaggacgggg gcgggggtgg ctactcaccg
     1141 ccctggtggc cctggccctg gccgccggga tcgtgggggg gctccagcat cagctgcgcc
     1201 ccctggtgga ggcggcggcc cgggcccagg cagccaacgc cctcaccgcc gtcatcgacc
     1261 gggccatcgc cgccgacctg gcggagcggg acgtggacta cggggacttc gtcaccatcc
     1321 agcgggacgc ctccggggcc atcaccgcca tgaccaccga tatggcggcc ctgaaccagc
     1381 tgcgggctca gctggtggcc cagatcctct ccgcactgga gggggtggac gcttccgaga
     1441 tccgcatccc cctgggcagc ctggtggaca gcgaactggt gtgggcccgg ggcccggagc
     1501 tgcgggtgcg ggccctgcgg gtggggacgg tgtcggcgga attccgcagc gacttctccc
     1561 aggcgggggt gaaccagacc ctgcaccgca tcgagctgga ggtggccgtc cccgtgacgc
     1621 tgatcctgcc cggcgggcag acggagctga ccatagagac cggcctgcgg gtggcggaga
     1681 ccgtcattgt tggggcggtg cccgacacat atctgaccct gaacggggcc gcgtgataca
     1741 gaggagagat gcctttcatg gatgaaaaac aggagctgga gtgcctgcgc cgggagctgg
     1801 tgcaggccaa ctatgagtat tatgtccagg acgcccccac catgacggac tacgactacg
     1861 accacaagct gcgccggctg gaagagctgg agggagccca cccggagtgg atcacccccg
     1921 actcccccac ccagcgggtg ggggggaagg ccctggacgc cttccagc
//
LOCUS       DBMDSJ010000341         1957 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13083_length_1957_cov_2.025862, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000341 DBMDSJ010000000
VERSION     DBMDSJ010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13083_length_1957_cov_2.025862"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="ACZPAQ_07905"
     CDS             complement(<1..563)
                     /locus_tag="ACZPAQ_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR0903952.1"
                     /translation="MMKHRTFALFLALGLSLSLLSGCGPKESGSTSQPGGSTSSSQGS
                     PDASQPDVSLPDASAPDTSAPGESAPEAPQPPVDEPEAVAALALDKSDFSLFKAGSSY
                     QLKATVSGVDAPKVTWTSSDETVAAVSQDGTVTAVARGTATITAAVEGTELRASCTVR
                     CKLPEDQPVSGGGNSSSQGSGNGGSSS"
     gene            complement(680..1207)
                     /locus_tag="ACZPAQ_07910"
     CDS             complement(680..1207)
                     /locus_tag="ACZPAQ_07910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR0903953.1"
                     /translation="MNEMLKLMHDRRSTRAYRPEPLQEAVIQQILEAGRFAPSGMNKQ
                     LNHFYVITDPNYLADLTALVSRKMENYRDKDFRYGAPVLVLVTNQKESTTCVQDASCA
                     MENMMLAAFSLGVGSCWINQLYRTREDREVQEKLAEIGVTPEEMPVAALALGWPAVDF
                     PGPREKTGNLVSWVR"
     gene            1415..1768
                     /locus_tag="ACZPAQ_07915"
     CDS             1415..1768
                     /locus_tag="ACZPAQ_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR0903954.1"
                     /translation="MEFVDHCEITQVHQGAVDQVRQVLPPDEVLSDLADLFKIFGDST
                     RIKILYALLRSELCVCDIADLLGLTQSAISHQLRVLRSSKLVKFRRDGKTLYYSLADE
                     HVCRIISQGMEHVEE"
     gene            1786..1947
                     /locus_tag="ACZPAQ_07920"
     CDS             1786..1947
                     /locus_tag="ACZPAQ_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903955.1"
                     /translation="MRITFAYALAEEGDRRAVAEETAVPACCAFLEGAVPPSNRVPSR
                     PAVSRWTRW"
BASE COUNT          334 a          614 c          601 g          408 t
ORIGIN      
        1 tgggaggagc tgcctccatt cccgctcccc tgggaggagc tgttcccgcc gccggaaacg
       61 ggctgatcct ccggcagctt gcagcggacg gtgcaggagg cacgcagctc cgtcccctcc
      121 acggcggcgg tgatggtggc tgtgccccgg gccactgcgg tgacggtgcc gtcctgggac
      181 acggccgcca ccgtctcatc gctggaggtc caggtcacct tgggggcatc caccccggac
      241 actgtggcct tcagctgata gctgctcccg gccttgaaca gggaaaagtc ggatttatcc
      301 agcgccagcg ccgctaccgc ctccggctcg tccaccggag gctggggggc ctccggtgcg
      361 ctctcccccg gtgcgctcgt gtccggggcg ctggcatccg gcagggaaac gtcgggctgg
      421 gaggcgtcgg ggctcccctg gctggaggag gtgctgccgc caggctggga ggtgctgccg
      481 ctctccttgg ggccgcagcc agagagcagg gagagggaaa ggccaagggc caaaaacagg
      541 gcaaaggtgc gatgcttcat catttcagat caaactcctt tttaggggga tcgatccgcc
      601 tgggatcgtc ttaccctaca ttggacgacg gtcctgccca aaaagttccc cctgatctga
      661 aaaatttttg ttccccgcct cagcggaccc aagacaccag gttcccggtc ttctcccggg
      721 ggccggggaa gtccacggcg ggccagccca gggccagtgc ggccaccggc atctcctccg
      781 gcgtcacgcc gatctccgcc agcttctcct ggacctcccg gtcctcccgg gtgcggtaga
      841 gctggttgat ccagcaggag cctactccta aggagaaggc ggccagcatc atgttctcca
      901 tggcacagga ggcgtcctgg acgcaggtgg tactctcctt ctggttggtc accagaacca
      961 gcaccggggc tccatagcgg aagtccttgt cccggtagtt ctccatcttg cggctgacca
     1021 gagcggtgag gtctgccaaa taatttggat ccgtgatcac ataaaaatgg ttcagctgct
     1081 tgttcatccc gctgggcgcg aaacggccgg cctccaggat ctgttggatc accgcctcct
     1141 gcagcggctc gggacggtag gcccgggtgc tgcgtcggtc gtgcatcagc ttcaacatct
     1201 cattcatgga acatgctcct tttctccgtc attctgtccc cggcgtccgt ttttggccct
     1261 ttttcggggg cagccctcat tatatcagcc ttttttccaa atctccaccg gtatttggaa
     1321 aatttaaaaa ttcctcttga caaatgggct cccttgtcat agtataatat atgaacaagc
     1381 gctcatatgt ttaatcgtta ggaaggagac cgctatggaa ttcgtcgatc actgtgagat
     1441 cacccaggtg caccagggcg cggtggacca ggtccgtcag gtcctgccgc cggatgaggt
     1501 gctcagcgac ttggccgacc tgttcaagat cttcggggat tccacccgga tcaagatcct
     1561 ctacgccctg ctccggtccg agctgtgcgt gtgcgacatc gctgatctgc tgggactgac
     1621 ccagtccgcc atctcccacc agctgcgggt cctgcggagc agcaagctgg tcaaattccg
     1681 ccgggatggc aagaccctct attattccct ggccgacgag cacgtctgcc gcatcatcag
     1741 ccaggggatg gaacacgtgg aagagtgagc ggtggctttc tttcattgcg gatcacgttt
     1801 gcctatgccc tggccgaaga gggggaccgc cgggcggtgg cggaggagac cgccgtgcca
     1861 gcctgctgcg cctttctgga gggggcggtg cctccctcca accgtgttcc gtcccgtcca
     1921 gctgtatccc ggtggactcg gtggtaatga gaccatg
//
LOCUS       DBMDSJ010000342         1956 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13098_length_1956_cov_1.316981, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000342 DBMDSJ010000000
VERSION     DBMDSJ010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13098_length_1956_cov_1.316981"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            484..1833
                     /gene="mgtE"
                     /locus_tag="ACZPAQ_07925"
     CDS             484..1833
                     /gene="mgtE"
                     /locus_tag="ACZPAQ_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR0903956.1"
                     /translation="MQNKKCLERCLELIRDKRYRELSAILAEQNPVDLADLLEGLPQE
                     QGALAFRTLPKEQAAEVFSNLPPDTQQMIVVSLPDRELAGILEELAMDDTVDLLEELP
                     AGVVKRVLKNARPETRRLINQFLKYPEQSVGSIMTAEFIDLKGTMTAAEAIRRIRRLG
                     GDCESIYTCYVTDGHRVLDGVVTLRELLLAPDNAPVRTLMEPNVITVRTTDDQELAVR
                     ALMRYDFLSLPVVDQEDRLVGVVTVDDVMDVMEEEATEDFQRMAAMAPSEKSYLKTGV
                     FALAKNRILWLLVLMISGMITGGILGRYEAAFSVMPLLVTFIPMLTDTGGNAGSQSST
                     LVIRGMAVGDIALSDFPKVLWKELRVSLLVGAVLSLVNLVRLLLSYPGNELVCVTVTL
                     SLFATVVLAKTIGGALPMLAKLVRADPAIMAAPLITTIVDALSLVLYFTIASRLLHL"
BASE COUNT          320 a          662 c          591 g          383 t
ORIGIN      
        1 ccccgcctct gcacggggtg tgccgcagct tccacgcccc catgcagggc ccgatatccc
       61 catcaggccg cacacatcgg ccatcagcac aggtgtatgg gaccgccctt ccgttttttt
      121 cccgttttct cttgcaatcc tctgtctgct gtgttatcct acgggtggag tatgattttc
      181 atctcccatc tgatctaaaa aggaggaaag gtaagtggac gcttacggcc gaagtctcaa
      241 atcggacagc gacagttctc tcccgggccc ccttgccttt tctccggcat agggagctct
      301 gcacagcgcc ccctgccgcc tggcttccgc acaggcggag gtctgttatg cgccccgccg
      361 gggcccttcc gctgtgctat gcgcccgctg agaatgtcgc cgcacaggtc tttcccgtct
      421 ccggggggcc ggtgcggctt tgtctgtccc attggaggct tcctgacaaa aaggaggttg
      481 ccaatgcaga acaagaaatg tttggagcgc tgcctggagc tgatccggga caagcgctac
      541 cgtgagctct ctgccattct ggcggagcag aacccggtgg atctggccga tcttctggag
      601 ggcctgcccc aggaacaggg cgcgctggcc ttccgcacgc tgcccaagga gcaggcggcg
      661 gaggtctttt ccaatctccc ccctgacacc cagcagatga tcgtcgtctc cctccccgac
      721 cgggagctgg ccggcatcct ggaggagctg gctatggacg acacggtgga tctgctggag
      781 gagctgcccg ccggcgtggt caagcgggtg ctgaaaaacg cccggcccga gacccgcagg
      841 ctcatcaacc agttcctcaa atatccggag cagtcggtgg gcagcatcat gaccgcggaa
      901 ttcatcgacc tgaaagggac catgaccgcg gccgaggcca tccgccggat ccgccgcctg
      961 gggggagact gcgaatccat ctacacctgc tatgtcaccg acggccaccg ggtgctggac
     1021 ggtgtagtca ccctgcggga gctgctgctg gcccccgaca acgcccccgt ccggacgctg
     1081 atggagccca acgtgatcac cgtccgcacc accgacgacc aggaactggc cgtccgggcc
     1141 ctgatgcggt acgactttct ctccctgccg gtggtggacc aggaggaccg gctggtcggg
     1201 gtggtcacgg tggacgacgt gatggatgtg atggaggagg aggccactga ggactttcag
     1261 cgcatggctg ctatggcccc ctcggaaaag tcctatctca agaccggcgt gttcgccctg
     1321 gccaaaaacc gcatcctgtg gctgctggtg ctgatgatct ccggcatgat caccggcggc
     1381 atcctgggtc gctatgaggc cgccttctcg gtgatgcccc tgctggtcac ctttatcccc
     1441 atgctcaccg acaccggcgg gaacgccggc agccagagca gcaccctggt catccgcggc
     1501 atggcggtgg gggacatcgc cctgtccgac ttccccaagg tgctctggaa ggagctgagg
     1561 gtcagcctgc tggtgggcgc ggtcctctcc ctggtcaacc tggtccgcct gctcctctcc
     1621 tacccgggaa acgagctggt gtgcgtcaca gtcaccctgt ccctgttcgc tacggtggtc
     1681 ctggccaaga ccatcggcgg agcgctgccc atgctggcca agctggtgcg ggccgacccc
     1741 gccatcatgg ccgcccctct gatcaccacc atcgtggacg ccctttccct ggtcctctac
     1801 ttcaccattg ccagccggct tctccacctg tgaggataca ccgccggaaa agctgggggg
     1861 cacggcttgc gccgtgcccc cctttccctg ctctcccgcc gctcagaatc ggttgagggg
     1921 gacagagccg tcgtccgtcc ccttttcgat gaatct
//
LOCUS       DBMDSJ010000343         1955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13104_length_1955_cov_1.505933, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000343 DBMDSJ010000000
VERSION     DBMDSJ010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13104_length_1955_cov_1.505933"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="ACZPAQ_07930"
     CDS             <1..184
                     /locus_tag="ACZPAQ_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR0903957.1"
                     /translation="FVHSFSAQDTDPAQVIAVTDYGPAVTAAVSSGNFYGTQFHPEKS
                     GEVGLTILRNFAGLNC"
     gene            224..946
                     /gene="hisA"
                     /locus_tag="ACZPAQ_07935"
     CDS             224..946
                     /gene="hisA"
                     /locus_tag="ACZPAQ_07935"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR0903958.1"
                     /translation="MYILPAIDLKDGKVVRLYKGDFNKVHQVAQDPLETARAFSEAGA
                     RWIHMVDLDGARGGTRINGGIVAEVTARSGLQVELGGGIRCMEDLEAVFQAGVGRAVI
                     GSAAVSDPDFVRAAAERYGERVAVGIDTQDGRVRTAGWVEDSGLDYLEFAKFMMEVGV
                     KNIIFTDIETDGALSGPSFARLEALKQVYTGNIVASGGVSSNEDIRRLARMGLYGAIV
                     GKAYYAGTVDLAQAVRDAGRQD"
     gene            956..1711
                     /gene="hisF"
                     /locus_tag="ACZPAQ_07940"
     CDS             956..1711
                     /gene="hisF"
                     /locus_tag="ACZPAQ_07940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR0903959.1"
                     /translation="MLAKRIIPCLDVRDGRVVKGVNFVNIRDAGDPVELARFYSDQGA
                     DEIVFLDITATSDGRATVADVVERTAEQVFVPLTVGGGIRTLEDFRQLLRAGADKISV
                     NSAAVKDPGLISRAAERFGSQCVVLAIDARRRPEGSYEVVVAGGRTPTGLDAVEWARR
                     GEALGAGEILLTSMDADGTKAGFDLEMTRAVTQAVSIPVIASGGCGSLEHFAQVFAET
                     DCDAALAASLFHFGELTVPQVKEYLRKRKIPVR"
     gene            1726..1920
                     /locus_tag="ACZPAQ_07945"
     CDS             1726..1920
                     /locus_tag="ACZPAQ_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903960.1"
                     /translation="MLDDLLELVLDVGGELLFSRKKGEQGKPEGQPSVPDGGRRQQKT
                     AFQPGKDPWEYQVKKPPWEK"
BASE COUNT          355 a          542 c          716 g          342 t
ORIGIN      
        1 cttcgtccac tccttcagcg cccaagacac cgatcctgcc caggtgatcg ctgtcaccga
       61 ttacgggccg gcagtgacag cggcggtgtc cagcgggaac ttctacggga cccagttcca
      121 cccggagaag agcggcgagg tggggctgac catcctgcgc aatttcgcag ggctgaactg
      181 ctgaggaccg gcaggatctg aacagaaagg gagcgacaga gccatgtata ttctgcccgc
      241 cattgactta aaagacggaa aagtagttcg cctttacaag ggggatttca acaaggtcca
      301 ccaggtggcc caggacccac tggagaccgc ccgtgccttt tccgaggccg gtgcccgctg
      361 gatccatatg gtggacctgg acggagccag gggaggcacc cgaatcaacg gcgggatcgt
      421 ggcggaggtc accgcccgct ccggtctcca ggtggagctg gggggcggca tccggtgcat
      481 ggaggacttg gaggcggtgt tccaggccgg ggtggggcgg gccgttattg gctcggctgc
      541 ggtgagcgac cctgattttg tccgcgctgc ggcggagcgc tatggggagc gcgtcgcggt
      601 ggggatcgac acccaggacg gcagagtcag gaccgcgggc tgggtggagg actctggatt
      661 ggactacttg gagtttgcaa agtttatgat ggaagttgga gtaaagaata ttatctttac
      721 agatattgag acggacggcg ccctctccgg tccctccttt gcccggctgg aggccctgaa
      781 acaggtgtac accgggaata ttgtggcctc cggcggcgta tcgtccaatg aggatatccg
      841 ccgtctggcc cgcatggggc tgtacggggc catcgtggga aaggcgtact acgcggggac
      901 ggtggatctg gcccaggcag tgcgggacgc cggccgtcag gattgaggtg atcgtatgct
      961 ggcaaagcgc atcatcccct gtctggacgt ccgggacggg cgtgtggtca agggagtcaa
     1021 ctttgtgaat atccgggacg ccggggaccc ggtggagctg gcccggttct actcggacca
     1081 gggggcggac gagatcgtct ttctggacat caccgctacc agcgacggcc gggccacagt
     1141 ggcggacgtg gtggagcgga ctgcggagca ggtgttcgtc cccctgaccg tgggcggcgg
     1201 catccgcacc ctggaggact tccggcagct cctccgggcg ggtgcggaca agatctccgt
     1261 gaattccgcc gcggtgaagg acccggggct gatctcccgg gcggcggagc gattcggctc
     1321 ccagtgcgtg gtgctggcca tcgatgcccg gcggcggccg gagggcagct atgaggtggt
     1381 ggtggccgga gggcgcaccc ccaccggcct ggacgccgta gagtgggccc ggaggggcga
     1441 ggcgctggga gcgggggaga tcctgctcac ctccatggac gcagacggca ccaaggcggg
     1501 ctttgatctg gagatgaccc gggccgtgac ccaggcggtc tccatccctg tcatcgcctc
     1561 cggaggttgc ggcagcctgg agcactttgc ccaggtcttc gcagagacag actgtgatgc
     1621 ggctctggcc gcctccctgt tccacttcgg ggagctgaca gtgccccagg tgaaggagta
     1681 tctgcggaag cggaagatcc cggtgcgctg agaggagcgg atgccatgct ggacgatctg
     1741 ctggagctgg tgctggacgt gggcggagag ctgctgttct cccgaaagaa gggggagcag
     1801 ggcaagccag agggccagcc gtctgtcccg gacggcggcc ggaggcagca gaagaccgct
     1861 tttcagcctg gaaaggaccc gtgggagtac caggtaaaaa aaccaccctg ggagaaatga
     1921 ggatagatat gtttgatttg gaccagctgt ttcaa
//
LOCUS       DBMDSJ010000344         1953 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13123_length_1953_cov_1.460043, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000344 DBMDSJ010000000
VERSION     DBMDSJ010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13123_length_1953_cov_1.460043"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            260..>1953
                     /gene="recJ"
                     /locus_tag="ACZPAQ_07950"
     CDS             260..>1953
                     /gene="recJ"
                     /locus_tag="ACZPAQ_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR0903961.1"
                     /translation="MKYEKWTVAPPDPAALAALEEAGISPLLAAVLSARGVRVPQEAR
                     ALLEGGETALEDPLALKDMAQAAGRVRLALERGETIAVYGDYDVDGITATCLLTDFLR
                     RKGGAVIPYIPDRLEEGYGLNREAVTALKEQGASLIVTVDCGITALEETAWARSLGID
                     VVITDHHECKSGLPAAQAVVDPRRSDCPSACKGLAGVGVALKLAMAINGPEGDGDVLA
                     EYCDLAAVGTVADVMPMTGENRTIVRLGLEELSHPRRLGLALLIREAGLEDKAITATS
                     IGYTLAPRINASGRMGRAQMAVELLLTRDRERGEQLAQELCALNRERQTIEGEIFRQC
                     VDRLDRAPQKGAVLLADPEWHQGVVGIVASRLTERYGCPAFMICLDHGMGKGSCRSWG
                     GVNLFERLAECSDLLEGFGGHALAAGFTVREENIPLLSRRLREGLERALGAGASPAAL
                     EADAEVEARELTVEAVADLDRLEPCGTGNPRPVLVLRGALIQSMSQVGRGRHLKLRLE
                     SKGTPLDAIFFSADGAELGLAPGGRVDVAFYPQINEFRGTRSVQLQVADLHPAPTRA"
BASE COUNT          297 a          606 c          734 g          316 t
ORIGIN      
        1 ctcttgaaga tctgggtgat atttaatcat agcacagatc ggagcgggaa gcaactgccg
       61 tctgcggcac gccgggcacc aaatcttttc ggggaccgct tttggcgcgt ttgatggctg
      121 catgcgcccc ttcgttccgc tcccgcggca cagacaaagg gggctgggtc ggaatttctc
      181 ttgaaggatc ttaaatagat gtgttataat actattctga gcaactatag ccgaagggcg
      241 ggaaaggagc gggtggcctg tgaagtatga aaaatggacg gtggcccctc ctgatccggc
      301 tgccctggct gccctggagg aggcgggaat ctcacctctg ctggcggcgg tgctgtccgc
      361 ccggggcgta cgtgtgccgc aggaggcccg ggccctgctg gagggtgggg agacggccct
      421 ggaggacccg ctggctctga aggatatggc acaggcggcc ggccgggtca ggctggctct
      481 ggagcgggga gagaccatcg cagtctacgg ggactacgat gtggatggca tcaccgccac
      541 ctgcctgctg accgatttcc tccgacggaa gggcggggca gtcatcccct atatcccaga
      601 ccggctggag gagggctacg gcctgaaccg ggaggcggtg acggccctga aggagcaggg
      661 cgcctccctg atcgtcacgg tggactgcgg cattactgcc ctggaggaga ccgcctgggc
      721 ccggtccctg gggatcgatg tggtcatcac ggaccaccac gagtgcaaga gcggcctgcc
      781 cgccgcccag gcggtggtgg acccccggcg cagcgactgc cccagcgcct gcaagggcct
      841 ggccggcgtg ggggtggccc tgaagctggc catggccatc aacggaccag aaggggacgg
      901 agacgtgctg gcggagtact gtgacctggc cgccgtgggg acggtggccg acgtgatgcc
      961 tatgaccggg gagaaccgga ccatcgtccg gctggggctg gaggagctct cccacccccg
     1021 ccggctgggc ctggccctgc tgatccggga ggcggggctg gaggacaagg ccatcaccgc
     1081 cacctccatc ggctacaccc tggcgccccg gatcaacgcc tccggccgga tgggccgggc
     1141 ccagatggcg gtggagctcc tcctcaccag ggaccgggag cggggggagc agctggccca
     1201 ggagctgtgc gccctgaacc gggagcggca gaccattgag ggcgagattt tccggcagtg
     1261 tgtggatcgg ctggaccggg ccccccagaa gggggcggtg ctgctggccg atccggagtg
     1321 gcaccagggc gtggtgggca tcgtggcctc ccgcctgacg gagcggtacg gctgccctgc
     1381 gtttatgatc tgcctggacc acgggatggg gaagggctct tgccgctcct ggggcggcgt
     1441 gaacctgttt gagcggctgg cagagtgcag tgacctgctg gagggcttcg gcggccacgc
     1501 cctggccgcc ggcttcaccg tgcgggagga gaacatcccg cttctctccc gccgcctgcg
     1561 ggagggtttg gagcgggccc tgggggccgg ggcatcccca gccgccctgg aggccgacgc
     1621 agaggtggag gcccgggagc tcactgtgga ggcggtggcc gatctggacc ggctggagcc
     1681 ctgcggtact ggcaaccccc ggcccgtcct ggtgctgcgg ggggccctga tccagtccat
     1741 gtcccaggtg gggcggggcc gccacctgaa gctgcggctg gagtccaagg ggaccccgct
     1801 ggacgccatt ttcttttccg ccgacggggc cgagctgggg ctggcccccg gcggccgggt
     1861 ggatgtggcc ttttatcccc agatcaatga gtttcgaggc acccgctcgg tgcagctcca
     1921 ggtggcggac ctgcatccgg cccccacccg ggc
//
LOCUS       DBMDSJ010000345         1951 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13147_length_1951_cov_1.334595, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000345 DBMDSJ010000000
VERSION     DBMDSJ010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13147_length_1951_cov_1.334595"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="ACZPAQ_07955"
     CDS             <1..208
                     /locus_tag="ACZPAQ_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR0903962.1"
                     /translation="YALHPALRDFYRLRIFLEATWPVRRARLLDRGGPELLARFQGEW
                     VPLEDRYFQACRVRDCCQLVLSGQ"
     gene            320..610
                     /locus_tag="ACZPAQ_07960"
     CDS             320..610
                     /locus_tag="ACZPAQ_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903963.1"
                     /translation="MYRVIVIVLAFMLGVFIFFGADSAKTRGQKKWVNVIGTLLVWIL
                     AVLGAGVLVLCCLAAWDYWKTGDAGTAVSLGITAVILAVAIYVLLRSYFKKT"
     gene            complement(804..>1300)
                     /locus_tag="ACZPAQ_07965"
     CDS             complement(804..>1300)
                     /locus_tag="ACZPAQ_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR0903964.1"
                     /translation="SEGVSGMKSRAFDTFMEDLCQSQSVPLRACYEKSFCLSADVTAA
                     YDPNFAEVYEKRNSAFVNYGMGLCKYTGARGKSGASDASAELVAYVRRVLDGAGVVWQ
                     MAELGKADQGGGGTVAVFMAERNIDTLDAGVPVLSMHAPFETVSKLDCYMTYKGMKAI
                     YEAV"
     assembly_gap    1301..1310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1311..>1951)
                     /locus_tag="ACZPAQ_07970"
     CDS             complement(<1311..>1951)
                     /locus_tag="ACZPAQ_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding metallopeptidase family protein"
                     /protein_id="HIR0903965.1"
                     /translation="EGAALEAYCTGYKQFLDAGKTERECVDRAIAQAEAAGFRAYVRG
                     MELKPGDRIYQVNRDKAIMLAVMGQKGLEHGVNIGAAHIDSPRLDLKQNPLYESDELG
                     FFKTHYYGGIRKYQWVTIPLELHGVVALKNGEVVRVSVGNGPDDPMFTIDDLLPHLAT
                     DQVKKPLGEAIPAESLNILVGSRPLRDDEGSDRVKLSVLELLNRKYGIVEEDF"
BASE COUNT          304 a          654 c          610 g          373 t
ORIGIN      
        1 ctacgccctc caccccgccc tgcgggactt ctaccgcctc cggatctttc tggaggccac
       61 ctggccggtg cggcgcgccc gccttctgga ccggggcggg ccggagctgc tggcccgctt
      121 ccagggggag tgggtcccgc tggaggaccg gtatttccag gcctgccggg tccgggactg
      181 ctgccagctg gtgctgagcg gtcaatgaca cgcgccccat ccccctgcag ctccgccttg
      241 caaagcgcgt caaggtatcg tatcatcaat agacatcaaa cagcttctgt tttctgcggt
      301 cggaaagagg aggtcagtga tgtacagggt catcgtgatc gtgctggcat ttatgctggg
      361 agttttcatt ttcttcggag ccgactccgc gaaaacccgc ggtcagaaaa agtgggtcaa
      421 cgtgatcggc accctgctcg tctggatatt ggcggtcctt ggggcaggtg ttctggtcct
      481 gtgctgtctg gcggcatggg actactggaa gaccggcgat gcaggaacgg ccgtctcctt
      541 agggatcacc gccgtgatcc tcgctgtcgc gatctatgtc ctgctgcggt cttatttcaa
      601 aaaaacatag ccgggaaaac agccccgtca ccgccccgca ggtcccgatc tgccggtgtg
      661 tccctgcccc gtgcggggcg ggcgcggcag gggtgccctt cccccggggc cgcaaagcga
      721 acttgggcgt tatggagcag aattggaaaa ggtgcagccg aaagatcggc tgcacctttt
      781 ccggtatcac ggggcatatg ggctcagact gcctcgtaga tggccttcat gcccttatag
      841 gtcatatagc agtccagctt ggacaccgtc tcaaaggggg catgcatgga cagcacgggc
      901 acgccggcgt ccagggtgtc gatgttccgc tccgccatga agacggccac cgtgccgccg
      961 ccgccctgat cggccttgcc cagctcggcc atctgccaca ccacgccggc gccgtccagc
     1021 acccggcgga cataggccac cagctcggcg gaggcgtcgg aggccccgga cttgccccgg
     1081 gccccggtgt acttgcacag gcccatgccg tagttgacaa aggcgctgtt gcgcttctcg
     1141 tacacctccg cgaagttggg gtcataggcc gccgtcacgt cggcggacag gcagaaggac
     1201 ttctcatagc aggcccgcag gggcacgctc tggctctggc acagatcctc catgaaggtg
     1261 tcgaaggccc tggacttcat gccggacacc ccctcggagc nnnnnnnnnn ggaagtcctc
     1321 ctccacgatg ccgtacttcc ggttgagcag ctccagcact gacagcttca cccggtcgct
     1381 gccctcgtcg tcccgcaggg ggcggctgcc caccaggatg ttcaggctct ccgcggggat
     1441 ggcctccccc aggggcttct tcacctggtc ggtggccagg tggggcagca ggtcgtcgat
     1501 ggtgaacatg gggtcgtccg gcccgttgcc caccgagacc cgcaccacct ctccgttctt
     1561 cagcgccacc acgccgtgga gctccagggg aatggtcacc cactggtact ttcggatgcc
     1621 gccgtagtag tgggtcttga agaagcccag ctcgtcgctc tcgtacaggg ggttctgctt
     1681 caggtccagc cggggggagt cgatgtgggc cgccccgatg ttgaccccgt gctccaggcc
     1741 tttctggccc atcaccgcca gcatgatggc cttgtcccgg ttcacctggt agatccggtc
     1801 cccgggcttc agctccatcc cccggacata ggcccggaag ccggcggcct ccgcctgggc
     1861 gatggcccgg tccacgcact cccgctcggt cttgcccgcg tccaggaact gcttgtagcc
     1921 ggtgcagtac gcctccagcg ccgcgccctc c
//
LOCUS       DBMDSJ010000346         1930 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13316_length_1930_cov_1.944779, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000346 DBMDSJ010000000
VERSION     DBMDSJ010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13316_length_1930_cov_1.944779"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /gene="ppk1"
                     /locus_tag="ACZPAQ_07975"
     CDS             complement(<1..1479)
                     /gene="ppk1"
                     /locus_tag="ACZPAQ_07975"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR0903966.1"
                     /translation="MQNRELSWLTFNERVLEEARDPSNPLAERLKFVSIFTSNLDEFF
                     MIRVGSLYDMAVMGDDQVDSRTGLLPSQQLEAIYRRAVLLMEQRDQVYAQLQTQLQAY
                     GVCEVSPGQLAGKDKDFLKTYFKTQLLPILSPQIVDINHPFPHLQNKSVYVVARLHGK
                     DRSLFGIVPVPPSAPEVVYLPGEGLRYVRTEQLLLDNLKRVFDPYGVAEKNCVCVTRN
                     ADIAPQDEGFDVDDDFRCQMQKLLHKRKRMAVVRLECAAPLSEALSAFLCKRCHVAPA
                     QIFLSRAPMRMGFVHPMLKKLPEPLHSALLYPPFSPQTPPKPKRETYLHLVQRRDLLL
                     SYPFESMEPFLRLIREAASDPSVLSIQITIYRLAQKARLVEYLCAAAENGKEVTAVIE
                     LRARFDEQNNIDWSERLEDAGCRVIYGFSDYKIHSKVCLITLKGKSGIQYVTQIGTGN
                     YNEKTSEQYTDLSLITADQGIGLDAAEFFKNMAVGNLEGSYHN"
     gene            complement(1503..>1930)
                     /locus_tag="ACZPAQ_07980"
     CDS             complement(1503..>1930)
                     /locus_tag="ACZPAQ_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR0903967.1"
                     /translation="RCVETLLPSPKEQAKIRKQVKKALDSAPLPDFPCPVICGVGGTI
                     RGAGKLCNYLYKLPAGNRTITLNMVDGLLERFSAPAKADLQILLKIVPERVHTLLPGL
                     VILSTLAHRCRGDAIEVSSYGVREGFLLTEVLKRRSVTV"
BASE COUNT          350 a          531 c          682 g          367 t
ORIGIN      
        1 gttgtggtag ctgccctcca gattgcccac agccatgttt ttgaaaaatt ccgctgcatc
       61 caggccgatg ccctgatccg cggtgatgag ggagaggtct gtgtactgct ctgaggtctt
      121 ctcgttgtag ttgccggtgc cgatctgggt gacgtactgg atgccggatt tgcctttcag
      181 ggtgatgaga cacaccttag agtggatctt atagtcgctg aacccgtaga tcacccggca
      241 gcccgcgtcc tccaggcgct cggaccagtc gatgttgttc tgctcatcaa agcgggcccg
      301 cagctcaatg acggcagtga cctccttacc gttttccgcc gccgcacaca gatactccac
      361 gagccgggcc ttctgggcca ggcggtagat ggtgatctgg atggagagga ccgacgggtc
      421 ggaggcggcc tcccggatca ggcggaggaa gggctccatg ctttcaaaag ggtagctgag
      481 aagcagatcc cggcgctgga ccaggtggag ataggtctcc cgcttgggct tggggggcgt
      541 ctggggggag aagggcgggt agagcagcgc actgtggagg ggttccggca gctttttcag
      601 catggggtgg acaaacccca tgcgcatggg agcccgggag aggaagatct gggcgggggc
      661 gacgtggcag cgcttgcaga gaaaggcgga cagggcctcg ctgagggggg cggcgcactc
      721 cagccggacc acggccatcc gtttgcgctt gtgaagcagc ttctgcattt ggcagcggaa
      781 gtcatcgtcc acatcaaagc cctcgtcctg gggggcgata tcggcgttgc gggtgacgca
      841 gacacagttt ttctccgcca cgccgtaggg gtcaaagacc cgcttcaggt tatccagaag
      901 cagctgttcg gtgcgcacat agcgcagtcc ctccccgggg agatagacca cctcgggggc
      961 ggagggaggc acagggacga tgccgaagag gctgcggtcc ttcccatgga ggcgggccac
     1021 cacatagacg ctcttgttct ggaggtgagg gaaggggtgg ttgatgtcca cgatctgagg
     1081 ggagaggatg gggagcagct gggtcttgaa gtaggtcttg agaaagtcct tgtccttccc
     1141 ggcgagctgg ccgggggaga cttcgcagac cccataggcc tggagctggg tctggagctg
     1201 ggcgtagacc tggtcccgct gctccatcag aaggactgcc cggcggtaga tggcctccag
     1261 ctgctgggag gggagcaggc cagtgcggct gtccacctgg tcgtccccca tgacagccat
     1321 gtcatagagg ctgcccaccc ggatcataaa gaattcgtcc aggttgctgg tgaagatgga
     1381 gacaaatttc agccgctcgg ccaggggatt ggagggatcg cgggcctctt ccagcacccg
     1441 ctcattgaac gtgagccagg agagctcgcg gttctgcatg taaggttggg gttcccgggc
     1501 catcagacag tcacgctcct tcgtttcaat acctcggtga gcaggaagcc ctcccggaca
     1561 ccgtagctgc tgacctcaat ggcgtcgccc cggcaccgat gggccagggt ggagaggatg
     1621 accagcccgg ggagcagggt gtggacccgc tcggggacga tcttcagcag gatctggagg
     1681 tccgccttag cgggagcgga gaagcgctcc aggaggccgt ccaccatgtt cagggtgatg
     1741 gtgcggttgc cggcgggcag cttgtagagg tagttgcaca gcttcccggc cccgcggatg
     1801 gtgccgccca ccccacagat caccggacag ggaaagtcgg gcaggggggc gctgtccagc
     1861 gcctttttca cctgcttgcg gatcttggcc tgttctttgg gggaggggag cagggtctcc
     1921 acacagcggt
//
LOCUS       DBMDSJ010000347         1927 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13350_length_1927_cov_1.341731, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000347 DBMDSJ010000000
VERSION     DBMDSJ010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13350_length_1927_cov_1.341731"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(344..1420)
                     /locus_tag="ACZPAQ_07985"
     CDS             complement(344..1420)
                     /locus_tag="ACZPAQ_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024600.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0903968.1"
                     /translation="MKPHIVTEDMRLRARDGQELEGKLYLPRDGVVSRLVVQVNGSGP
                     QTCNTFRDRGDGTTYSFCGFFGEELAGRGLGFFCYSTRGCTDGDQGPLYCRVDRAVYR
                     SYTPQTCVADLEDWTALLQAHPRCAGAELLLLGWSEGTMIAPAVAAGGAVPLAGLLLA
                     GYANGTMEEALDWQQRGLGLYTMLCQYFDADGDGSISREEFETGPGAVREALEATFSG
                     LDLDGDGRITPADLEAVYAGSRAEIFRAIEAGDDEWLWEHYPVPLTTNWFRGHRRFPP
                     NRETLPRLDLPIHILQGRWDANTPAEDALAIQETFRRLGKDNLAVHIYPDAGHALGFE
                     QYLATGELPPALTDLFELCQGLGR"
     gene            complement(1568..>1927)
                     /locus_tag="ACZPAQ_07990"
     CDS             complement(1568..>1927)
                     /locus_tag="ACZPAQ_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009270939.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR0903969.1"
                     /translation="TIVLATVKGDIHDIGKNIVKVLLENYGYTVVDLGKDVDPAAVAE
                     AVRKHRAPLVGLSALMTTTLKSMADTIALLHLEGLPCKIMVGGAVLTPEYASQIQADF
                     YARDAKESVDIAKRVIG"
BASE COUNT          302 a          691 c          606 g          328 t
ORIGIN      
        1 cctcccccgg cggcagtgtc cggtacagcg ccagacaggc caccgccagg gcgacgaccg
       61 ccagcaccag ggccagcttc cccacccagc tcccgctccg gcggggcggc tccacccggg
      121 acgggcgccg ctcctcctcc cgctcctcca atgggatcaa ggtctctgcc ataatgaact
      181 cctttccggc tccgcacgcg gattcgactt ttttctgttc cgattatagc agacgggaca
      241 ggagggcaca aggggctttc cctttcagcc gcgggctcct cctccgggcc ccggagtccg
      301 gaaaaaagcg ggagccggag aggcccgctc tccggctcct gttctatcgt cccagcccct
      361 ggcacagctc aaagagatcg gtgagggcgg ggggcagttc cccggtggcc agatactgct
      421 caaagcccag ggcgtgtcct gcgtcagggt agatgtggac ggcgaggttg tccttcccca
      481 gcctccggaa ggtctcctgg atggccaggg cgtcctccgc aggggtgttg gcgtcccacc
      541 tcccctggag gatatggatg ggcaggtcca gacggggcag ggtctcccgg ttggggggaa
      601 acctccggtg gccccggaac cagttggtgg tgagggggac tgggtagtgt tcccaaagcc
      661 actcgtcgtc ccccgcctcg atggcccgga agatctccgc ccggctcccg gcatacaccg
      721 cctccaggtc cgccggggtg atccggccgt ccccgtccag gtccagcccg gagaaggtcg
      781 cctccagggc ctcccgcacc gctcccggcc ccgtctcaaa ctcctcccgg gagatgctcc
      841 catctccgtc ggcgtcaaaa tactggcaga gcatggtgta cagccccagc ccccgctgct
      901 gccagtccag ggcctcctcc atggtcccgt tggcgtagcc cgccagcagc agtccggcca
      961 ggggtaccgc tccccctgca gccacagccg gagcgatcat ggtcccctcg ctccacccca
     1021 gcagcaggag ctccgcccca gcacagcggg ggtgggcctg aaggagggcg gtccagtcct
     1081 ccaggtcggc cacacaggtc tgaggggtgt aggagcggta gaccgcccgg tccactcggc
     1141 agtagagtgg accctgatcc ccatcggtgc acccgcgggt gctgtaacag aagaagccca
     1201 gtccccgacc ggcaagctcc tccccaaaga agccgcagaa gctgtacgtg gtcccgtctc
     1261 cccggtcccg gaaggtgttg caggtctggg ggccggagcc gttgacctgg accaccagcc
     1321 gggagacgac gccatcccgg ggcagataca gctttccctc cagctcctgc ccgtcccggg
     1381 cccggagcct catgtcctct gtgacaatgt gtggtttcat ggccttcgcc tcctctctgt
     1441 ccggaggata gcagatccct ggctcacacg caaggggatg ggagctgtca ggcctttttc
     1501 cgccggtaag cggcacagaa aggccgggag gacggcttga cagcccctcc cggccccatt
     1561 gcggtcgtta cccgatgacc cgcttggcga tgtccacgct ctccttggca tcccgggcgt
     1621 agaagtccgc ctggatctgg gacgcatact ccggggtgag caccgccccc cccaccatga
     1681 tcttacaggg caggccctcc agatggagca gggcgatggt gtccgccatg ctcttcaggg
     1741 tggtggtcat cagggcggac agccccacca gaggggcccg gtgcttccgc accgcctccg
     1801 ccacggcggc tgggtccacg tccttcccca ggtccaccac ggtgtagccg tagttttcca
     1861 gcagcacctt gacgatgttc ttgccgatgt cgtggatgtc ccctttcacg gtggccagca
     1921 cgatggt
//
LOCUS       DBMDSJ010000348         1926 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13361_length_1926_cov_1.319452, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000348 DBMDSJ010000000
VERSION     DBMDSJ010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13361_length_1926_cov_1.319452"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            216..>1926
                     /gene="iorA"
                     /locus_tag="ACZPAQ_07995"
     CDS             216..>1926
                     /gene="iorA"
                     /locus_tag="ACZPAQ_07995"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR0903970.1"
                     /translation="MKQLMLGNAAVARGLYEAGCCVISSYPGTPSTEITEEAAKFDDI
                     YCEWAPNEKVAMEVAFGASLAGKRSFCAMKHVGLNVAADPLFTVAYTGVNAGMVIAVA
                     DDPGMHSSQNEQDSRHYAIAAKLPMLEPADSAEALAFTRRAYEISEEFDTPVILKMCT
                     RVAHSQSIVETSPRTEGPERPYEKNIAKYVMMPGNAIRRHPVVEDRMRRLAQYAETCD
                     LNRVEMGDTKLGIITSSTSYQYVKEVFGEEASVLKLGMVHPMPEGLIRDFAAKVDRLV
                     VVEELDPIIENYCRQLGLTVSGKEALPLEGEFSQNLVAAKLGGAVHTGTALEDAIPPR
                     PPVMCAGCPHRGLFYTLAKNKCTVLGDIGCYTLGAVAPLNAMEMTLCMGASISGIHGF
                     NKALGEKSEGKTVAVIGDSTFMHSGMTGLANIAYNQSNSTVIILDNSITGMTGHQQNP
                     TTGYNIKGEPAGKIDLEQLCRAMGFERVRVVDPYDLKATDQAVKEELAAREPSVIISR
                     RPCALLKYVKHQAPLAVDTDKCVGCKSCMRIGCPAISMKEGKAHVDNTLCVGCGVCQQ
                     LCPV"
BASE COUNT          382 a          613 c          623 g          308 t
ORIGIN      
        1 ggtctacctg gacatcctgg agaaaacggg ggccagcagt gccaccatca gccgggtccg
       61 tcgggccatg ctggacaacg gcgccggcgg tgtgatgcag gacgtcatta tccgggaggg
      121 cctgggagag cccaaagcgg atcctgagcc gtgacggtgc gggaagccgt ccggctttgc
      181 ggggaagaac aatacagaaa gcagggaaca gattcatgaa acaactgatg ttgggcaacg
      241 ccgccgttgc ccggggactt tacgaggcgg gctgctgcgt gatctccagc taccccggca
      301 cccccagcac cgagatcacc gaggaggccg ccaaattcga cgatatttac tgtgagtggg
      361 cccccaatga gaaggtagcc atggaggtgg ctttcggtgc cagcctggca ggaaagcggt
      421 ccttctgcgc catgaagcac gtgggcctca acgtggccgc tgacccgctg ttcaccgtgg
      481 cctataccgg cgtcaatgcg ggcatggtca tcgcggtggc cgacgacccg gggatgcact
      541 cctcccagaa cgagcaggac tcccggcact acgccatcgc cgccaagctg cccatgctgg
      601 aacccgccga ctccgccgag gccctggcct tcaccaggcg ggcctatgag atctccgagg
      661 agttcgacac tccggtgatc ctgaagatgt gcacccgggt ggcccactcc cagtccatcg
      721 tggagaccag cccccggacc gaggggccgg agcgtcccta tgagaagaac atcgccaagt
      781 atgtaatgat gccgggcaat gccatccgcc gccacccggt ggtggaggac cggatgcgcc
      841 gcctggccca gtacgccgag acctgcgatc tgaaccgggt tgagatgggg gacaccaagc
      901 tgggcatcat cacctccagc acctcctacc agtatgtgaa ggaggtcttt ggggaggaag
      961 cctctgtcct caagctgggg atggtccacc ctatgcccga gggactgatc cgggacttcg
     1021 ccgccaaggt ggaccgactg gtggtggtgg aggagctgga ccccatcatc gaaaactact
     1081 gccgtcagct gggcctgacc gtttccggca aggaggccct gcccctggag ggggagttct
     1141 cccagaacct ggtggccgcc aagctgggcg gcgcggtaca taccggcacc gccctggagg
     1201 acgccatccc gccccgcccg ccggtgatgt gcgccggctg tcctcaccgc ggactgttct
     1261 acaccctggc aaaaaacaag tgtaccgtgc tgggcgacat cggctgttac accctggggg
     1321 cggtggcccc tctgaacgcc atggagatga ccctctgcat gggcgcctcc atcagcggca
     1381 tccacggctt caacaaggca ttgggggaga aaagcgaggg gaagacggtg gccgtcatcg
     1441 gagactccac cttcatgcac tctggcatga ccggcctggc caacatcgcc tataaccagt
     1501 ccaactccac ggtcatcatc ctggacaact ccatcaccgg catgaccggc caccagcaga
     1561 accccaccac cggctacaac atcaaggggg agccggcggg aaagatcgat ttggagcagc
     1621 tgtgccgggc catgggcttt gaacgggtcc gggtggtgga cccctatgac ctgaaggcca
     1681 ccgaccaggc ggtgaaggag gaactggccg cccgggagcc ctccgtcatc atcagccgcc
     1741 ggccctgcgc tctgctgaag tatgtaaagc accaggcccc tctggcggtg gatacggaca
     1801 agtgcgtggg ctgcaagagc tgcatgcgca tcggctgccc cgctatctcc atgaaggagg
     1861 gcaaggccca tgtggacaac accctgtgcg tgggctgcgg cgtgtgccag cagctgtgtc
     1921 cggtgg
//
LOCUS       DBMDSJ010000349         1913 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13482_length_1913_cov_1.577263, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000349 DBMDSJ010000000
VERSION     DBMDSJ010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13482_length_1913_cov_1.577263"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="ACZPAQ_08000"
     CDS             <1..741
                     /locus_tag="ACZPAQ_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903971.1"
                     /translation="EGYNERRNQGMSAEIEDYGGLLGEVMQNVNRIAGENAAYMENLK
                     EQYKREALSAVLLGEATSYIFSSEDQAQLQAMRKEFSEASTQYEATGSQEAALKMEAL
                     TTTAEAMAQAAYEASDQYQMMQDAEADSLAALRDNTSALEAATNAYSLSQERTKGTAA
                     RRNYWGPGAATGLERVPYNNFPAVLHEGERVLTAREARNYARGGGSGGIQITMNGTVI
                     REEADIDRVAQRLLNKILSAKTAGVYGE"
     gene            738..1388
                     /locus_tag="ACZPAQ_08005"
     CDS             738..1388
                     /locus_tag="ACZPAQ_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR0903972.1"
                     /translation="MSLNFSFLADGEELMLPVPPLPFGWGVAQNVREVTVNGTGTVYL
                     PGDPAAFSGPLEALLPAREYPFNAPGAVLDPYYYIQKFSKWVTGKKIVRYVVPGVVNE
                     RVVIEEISYEERDGTGDVYAKIYLKASPPLEAVTTESSAGTDSGGRSEPETDQGLQTY
                     TVVTGDCLSVICRRFYGNGTAKYYNALAAYNGIKNPHLIHPGTVLTIPPKAQLGVS"
     gene            1390..>1913
                     /locus_tag="ACZPAQ_08010"
     CDS             1390..>1913
                     /locus_tag="ACZPAQ_08010"
                     /inference="COORDINATES: protein motif:HMM:NF048266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ/YqbQ family protein"
                     /protein_id="HIR0903973.1"
                     /translation="MILKLTHGKTTVENAWGLVGSVTWSGDKQRAARTLTFELAASQE
                     DPNLPAVECPPGTIASLWGDDGAPLFQGQVVRRSLSDAEASIPVTVHDRGMFLANNDG
                     TEKIRDETPETAVRRICQKYGIPVGSLASTGVKVRRKFTKTSLWNIVVTLYTLASRQT
                     GKQYMVRFEWDKLVV"
BASE COUNT          394 a          556 c          661 g          302 t
ORIGIN      
        1 gagggctaca acgagagacg gaaccagggg atgagcgccg aaatcgagga ttatggcgga
       61 ctgctgggcg aggtgatgca gaatgtgaac cgcatcgccg gcgagaacgc cgcctacatg
      121 gaaaacctga aggagcagta caagcgggag gccctgtccg ccgtcctgct gggcgaggcg
      181 acctcttaca tcttctccag cgaagatcag gcccagctgc aggccatgcg gaaggagttc
      241 agcgaggcca gcacccaata tgaagccaca gggagccagg aggccgcgct gaagatggag
      301 gctttgacca ccacggcgga ggccatggcc caggcggctt atgaggccag cgaccaatac
      361 cagatgatgc aggacgcgga ggctgactcc ctggcggcgc tgcgggataa tacctctgcc
      421 ctggaggccg ccaccaatgc ctacagcttg agccaggagc ggaccaaggg cacggccgca
      481 cgacggaact actgggggcc tggagcggcc acaggcttgg agcgggtgcc ctacaacaac
      541 ttccctgctg tgcttcacga gggagagcgg gtgctgacgg cccgtgaggc ccggaactat
      601 gccagaggcg gcggttccgg cggcatccag atcaccatga acggcaccgt gatccgggag
      661 gaggccgaca tcgaccgggt agcccagcgc cttttgaata agatcctgtc ggcaaagacg
      721 gcgggggtgt atggagaatg agcctgaatt tttccttttt ggcggacggc gaggagctga
      781 tgctccctgt gcctcccctg ccctttggct ggggcgtggc ccagaacgtc cgtgaggtga
      841 cggtaaacgg caccgggacc gtctacctgc ccggtgaccc ggcggccttc tccggcccgc
      901 tggaggccct gctgccggcc cgggaatatc ctttcaacgc cccgggagcg gtgctggacc
      961 cctattatta catccagaag ttttccaagt gggtgaccgg caagaagatt gtccggtatg
     1021 tggtgcccgg cgtggtcaat gagcgggtgg tcattgaaga gatctcctat gaggaacggg
     1081 acggaaccgg agacgtctac gcgaagatct acctgaaggc atccccgccc ctggaggctg
     1141 tgaccaccga gagcagcgcc gggacggaca gcggcggccg gagtgagccg gagacggacc
     1201 aggggctaca gacctacacg gtggtgacgg gggactgtct gtcggtgatc tgccggcgct
     1261 tttacggcaa tggcaccgcc aaatattaca atgccctggc cgcctacaac ggcatcaaga
     1321 atccgcacct gatccatccg gggaccgtgc tgaccatccc cccgaaggcg cagctggggg
     1381 tgagctgaca tgatcctgaa gctgacccac gggaagacca cagtggagaa cgcctggggg
     1441 ctggtgggga gtgtgacctg gagcggagac aagcagcggg cagccaggac gctgaccttt
     1501 gagctggccg ccagccagga ggaccccaac ctgccggcgg tagagtgtcc gccggggacc
     1561 attgccagcc tgtggggaga tgacggggcc ccgctgttcc agggccaggt ggtgcgccgg
     1621 tccctgtctg acgcggaggc gtccatccca gtgaccgtcc atgaccgtgg gatgttcctg
     1681 gccaacaatg acggtactga gaagatccgc gacgagaccc cggagacggc ggtgcggcgg
     1741 atctgccaga agtacggcat cccggtgggc agtttggcct ccaccggagt gaaggtccgg
     1801 aggaaattta caaaaacctc gctttggaac atcgtggtga cactctacac cctggcctcc
     1861 cggcagacgg gcaagcagta catggtccgg tttgagtggg acaagctggt ggt
//
LOCUS       DBMDSJ010000350         1906 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13542_length_1906_cov_1.136842, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000350 DBMDSJ010000000
VERSION     DBMDSJ010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13542_length_1906_cov_1.136842"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="ACZPAQ_08015"
     CDS             complement(<1..395)
                     /locus_tag="ACZPAQ_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR0903974.1"
                     /translation="MRQRQTGQADKLGIYIHIPFCRSKCDYCDFYSLAGREDRMDAYQ
                     KALLAHIRETAPLAQGIPVDTVYFGGGTPSWYGARRIQELLSALSKLFQVEKDAEITV
                     EANPDSVDLRALRRLRKAGVNRLSLGMQSG"
     gene            768..971
                     /locus_tag="ACZPAQ_08020"
     CDS             768..971
                     /locus_tag="ACZPAQ_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0903975.1"
                     /translation="MKNRIAQLRKERRITQEELADAVEVTRQTIISLENGRYNASLLL
                     AHKIAKYFGSSIEEIFLFEEEET"
     gene            968..1381
                     /locus_tag="ACZPAQ_08025"
     CDS             968..1381
                     /locus_tag="ACZPAQ_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903976.1"
                     /translation="MKKLKPILLAAAALGGLALMAAAALLDLPGGITGLMCGMGGALL
                     GLGGSSVIMGAVERHMSPQERKELQRSETDERNIAIREKAAQSSWYWTLYLLWVPFVI
                     SLVREETLWILLSSAVTVLHCVFYMVNMGRWAKKL"
     gene            1394..1855
                     /locus_tag="ACZPAQ_08030"
     CDS             1394..1855
                     /locus_tag="ACZPAQ_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903977.1"
                     /translation="MLIKTTGRGPDYGKNLIQRRWAFLALLAVGATGLVCYFFLVPGS
                     GISGHARGFYLGGASGITGAALANLLRNEWMLRHPERWKKARIQETDERSQRLTAQAA
                     QLAGVATFFLQALAAFVLVAVDPMLVKVLLGSMLAYILFFLGARAALARRR"
BASE COUNT          354 a          588 c          615 g          349 t
ORIGIN      
        1 ccggactgca tccctaggga caggcggttg acccccgcct tccgcaggcg gcgcagggca
       61 cgcaggtcca cgctgtcagg gtttgcctcc accgtgatct ctgcgtcctt ctccacctga
      121 aaaagcttgc tcagggcgga gagcagctcc tggatgcgcc gggccccgta ccagctgggg
      181 gtgcccccgc cgaaatagac ggtgtctacc gggatgccct gggccagggg cgcagtctcc
      241 cggatgtggg ccagcagcgc cttctgatag gcgtccatcc ggtcctcccg ccccgccagg
      301 gagtagaagt cgcagtagtc gcacttgctc cggcagaagg ggatatggat gtaaatgccc
      361 agcttgtccg cctgtccggt ctgtctctgt ctcatggggc cgccctcctt atgatctcaa
      421 aggtattata gcggagctga gcccagttgc aaggggcagg cagaaatttt gtggacccgg
      481 acagggcagg cggcggaagg gagggcactt tttcatcaaa tatgccccgc cgccggggct
      541 ggcatgaaac ggcggtcccg ccctgcgggg cgggaccctt ttgggcgcat cctgcggatg
      601 gaggcgggcg gggcaagctc ttccctcctc cgccccagaa gtgctccaag atccggtccc
      661 cagaaaaaag aagggcaaaa tagtaaagcc tgcttgacac aaaatgtaaa gcgtgctata
      721 ctctagatgt aaagcaagct aaacattttt gcgtgaggag gaccgccgtg aaaaaccgca
      781 tcgctcagct ccgaaaggag cggcgcatca cccaggagga gctggccgac gctgtggagg
      841 tcacccggca gaccatcatc tccctggaaa acgggcgcta caacgcctcg ctgctgctgg
      901 cccacaagat cgccaaatat ttcgggagct ccattgagga gatatttctg ttcgaggagg
      961 aagagacatg aagaagctga aaccgatcct gctcgccgcc gccgcgctgg gcgggctggc
     1021 cctgatggcg gcggccgccc tgctggatct gcccggcggg atcaccggcc tgatgtgcgg
     1081 catgggcgga gccctgctgg gcctgggggg ctccagcgtg atcatgggcg ccgtggagcg
     1141 gcacatgtcc ccccaggagc ggaaggagct ccagcgcagc gagaccgacg agcgcaacat
     1201 cgccatccgg gagaaggccg ctcagtccag ctggtactgg accctgtacc tgctgtgggt
     1261 cccctttgtg atctctctgg tgcgggagga gacgctttgg atccttctct cctccgccgt
     1321 aacggtcctc cactgcgtct tttatatggt caatatgggc cgctgggcca aaaagctgta
     1381 aggaggaaaa atcatgctca taaaaacgac cggacggggc cctgactatg gaaaaaatct
     1441 gatccagcgc cgctgggcat tcctcgccct cctggcggtg ggcgcgacgg gcctggtgtg
     1501 ctatttcttc ctggtgcccg gcagcggcat cagcggccac gcccgaggct tttatctggg
     1561 gggcgccagc ggcatcaccg gcgccgcact ggccaacctg ctgcgcaacg agtggatgct
     1621 ccgccacccg gagcggtgga aaaaggccag gattcaggag accgacgagc gcagccagcg
     1681 tctcaccgcc caggcggccc agctggcggg ggtggcgaca ttttttctcc aggccctggc
     1741 cgcctttgtc ctggtggcag tggacccgat gctggtgaag gtgttgctgg gctccatgct
     1801 ggcctacatc ctgttcttcc ttggcgcccg ggccgccctg gcccggcggc ggtgagaact
     1861 ttatcctgga aaggaggaag ccctgtgtcc tgtccaaacc caattc
//
LOCUS       DBMDSJ010000351         1901 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13581_length_1901_cov_2.041111, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000351 DBMDSJ010000000
VERSION     DBMDSJ010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13581_length_1901_cov_2.041111"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="ACZPAQ_08035"
     CDS             <1..657
                     /locus_tag="ACZPAQ_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR0903978.1"
                     /translation="IVFTSLAPLFSKGEDDPGGSQADPGASTSQTGPDASSTPSGSQG
                     DISQPEPEPPVTVDVTSLTLDKSDFTLQAGESATIQAAVVPADAEITWSSSDESAATV
                     SPEGVVSNVNTGSSQVKVTITATAGDKTAECVVFCRAGGGAGSSSSGTTGTVTGAGSG
                     LFVRSGPGSGNEAIATILEGNTVTILEDMGNGWYRISFIGSGGRATEGYVSKDYIKVS
                     "
     gene            781..1155
                     /locus_tag="ACZPAQ_08040"
     CDS             781..1155
                     /locus_tag="ACZPAQ_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR0903979.1"
                     /translation="MKQISRLPDAELEVMQALWAVPEYPARTSDLAARLDRDWKAPTL
                     LKLLSRLEERGFVRGEKEGRSNGYTPLVNRPDYLAAESRSFLERVHGGSLSSLMAALY
                     PEKELSREDIAELERILEKGGK"
     gene            1157..>1901
                     /locus_tag="ACZPAQ_08045"
     CDS             1157..>1901
                     /locus_tag="ACZPAQ_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903980.1"
                     /translation="MLEQLTALLAASLGGGGAAILVLLTLGPLLTRKVSPHWRYWAWA
                     VVAVSLMAYPILGPVLGALFPAPIQLDVPQMVAHGAYDRYNAYHLDNQRIVEAGAGGA
                     GGTNTYMGGEPMWYSHHVHYTDRQGREVSIRDNDYLRTVTVEGVTSYTVHWTGVIYGA
                     YRGVAVLFLLWMAAGYGLGRRRLLRWSTPAGEEDLAALERARQETGCRKKASLYRCPK
                     AGSPLLLGFAHPVILLPETLQEGGREAALA"
BASE COUNT          346 a          591 c          644 g          320 t
ORIGIN      
        1 attgtcttca cctccctggc tcccctgttc agcaagggag aggatgatcc cggcgggagc
       61 caggcagacc ccggcgcctc caccagccag accggtcctg atgcctccag cacccccagc
      121 ggctcccagg gagacatctc ccagcctgag cccgagcccc cggtgacggt ggatgtgacc
      181 tccctgacgc tggataagag cgacttcacc cttcaggccg gggagtccgc caccatccag
      241 gccgccgtgg tccccgccga cgcagagatc acctggagca gcagcgacga gtctgcggcc
      301 accgtcagcc cggagggcgt ggtgtccaac gtgaacaccg gctcctccca ggtgaaggtg
      361 accatcaccg ccaccgccgg ggacaagacg gcggaatgtg tcgtcttctg ccgcgccggc
      421 ggcggggccg ggtcgtcctc ctccggtacc accggaacag tgaccggcgc aggcagcggc
      481 ctgttcgtcc gctccggacc gggcagcggc aatgaggcca tcgccaccat tctggaggga
      541 aacaccgtta ccattctgga ggacatgggc aacggctggt accggatcag ctttatcgga
      601 agcggcggca gagccacaga gggctatgtg tccaaagatt atatcaaggt ctcctgaact
      661 gctgtgttcc gggccgtccc ccaggggacg gcccggaatt tttgcagttt tcggcttgac
      721 aaaataatta gacagttgta aaatctaaaa agacaaacgt ctaataaagg gggagatgac
      781 atgaagcaga tctcacgtct gcccgacgcg gagctggagg tgatgcaggc cctgtgggcc
      841 gtcccagagt atccggcccg caccagcgat cttgcggctc ggctggaccg ggactggaaa
      901 gcgcccaccc tgctcaagct cctgtcccgg ctggaggagc ggggctttgt ccggggggag
      961 aaggaggggc ggtccaacgg ctacacccct ctggtgaacc ggccggacta tctggctgcg
     1021 gagagccgat ccttcctgga gcgggtccac ggcggttccc tgtccagcct gatggctgcc
     1081 ctgtacccag agaaggagct gagccgggag gacatcgccg agctggaacg catcctggag
     1141 aaagggggaa aatgaggtgc tggagcaact gacggccctg ctggcggcca gcctgggggg
     1201 tggtggggca gccatcctgg tgctgctgac cctgggcccc ctgctgacgc ggaaggtctc
     1261 accgcactgg cggtattggg cctgggccgt ggtggctgtc agcctgatgg cctatcccat
     1321 tctcggcccc gtattggggg ccctctttcc cgctcccatt cagctggatg tcccccagat
     1381 ggtggctcat ggtgcctatg accggtacaa cgcctatcat ttggataacc agcggatcgt
     1441 ggaggccgga gcagggggag caggggggac aaatacctac atgggcggag aaccaatgtg
     1501 gtacagtcat cacgtccact atacagaccg gcagggcagg gaggtgagca tccgggacaa
     1561 cgactacctc cgcaccgtca ctgtggaggg ggtgacctct tacaccgtcc actggacggg
     1621 ggtgatctat ggagcatatc ggggggtggc cgtcctcttt ctcctgtgga tggctgcggg
     1681 ctatggactg ggccgccggc gactgctgcg gtggagcacc ccggcggggg aggaggatct
     1741 ggccgcgctg gagcgggcca ggcaggagac gggctgccgg aaaaaggcgt ccctgtaccg
     1801 ctgccccaag gcaggctccc ccctgctgct gggctttgcc cacccggtga tccttctgcc
     1861 tgagactctg caggaggggg gacgggaggc cgccctggcc c
//
LOCUS       DBMDSJ010000352         1896 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13626_length_1896_cov_1.262396, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000352 DBMDSJ010000000
VERSION     DBMDSJ010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13626_length_1896_cov_1.262396"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(110..838)
                     /locus_tag="ACZPAQ_08050"
     CDS             complement(110..838)
                     /locus_tag="ACZPAQ_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR0903981.1"
                     /translation="MNGTPTAPELLEAARQGDNRACEQVLEENAGLIWSIVRRYYGRG
                     VEPDDLYQLGCLGFLKAVRGFDPSFGTQFSTYAVPKIAGEIRRFLRDDGTVKVSRGMK
                     ERGAAVWGARRRLVAEMGREPTLSELAAETGLTPEEIAAAETASDAVASLQAETGEGG
                     LTLEGMLGTGGMEEDVVERLTLRSAMEELPEREQQVLLLRYYKNLTQIQCARVLGVSQ
                     VQISRLERRAVDRLRKILCEEEAF"
     gene            complement(835..1275)
                     /gene="spoIIAB"
                     /locus_tag="ACZPAQ_08055"
     CDS             complement(835..1275)
                     /gene="spoIIAB"
                     /locus_tag="ACZPAQ_08055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR0903982.1"
                     /translation="MKVENYAALEFPSRSANEGFARAAAACFAAQLDPTLEEINDIKT
                     AVSEAVTNAIVHAYPDRLGKVALKLRLKEGGILEILVRDWGVGIADVEQARTPLFTTG
                     SEERSGMGFTIMESFMDVVKVRSTPGKGTQVTMRKRISPRIGGR"
     gene            complement(1272..1619)
                     /locus_tag="ACZPAQ_08060"
     CDS             complement(1272..1619)
                     /locus_tag="ACZPAQ_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981684.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HIR0903983.1"
                     /translation="MTVDCVSEGRTLTAAVRGELDHHRAKEVMTELDEQIDMALPRRL
                     TLDLSGLSFTDSSGIAVLIRVSRRMEQIKGELIVRGVPPQPWKVFQAAGLHKLIRFEQ
                     EEPAAAGEEDVHK"
BASE COUNT          314 a          689 c          501 g          392 t
ORIGIN      
        1 catctctggt acgggcccgg agtatttacg gctgcgaaaa agtcctccag ggactttttc
       61 cgcagcctgc atcctattat ttttctgccc cgtctggggc agagaagtct cagaacgctt
      121 cttcctcgca caggatcttc cgcagccggt ccaccgcccg ccgctccagc cgtgagatct
      181 ggacttggga gacccccagg actctggcac actggatctg cgtgaggttt ttgtaatagc
      241 gcagcagcag cacttgctgt tcccgctccg gcagctcctc catggcggaa cggagggtca
      301 gccgctccac cacgtcctcc tccatcccgc cggtgcccag catcccctcc agggtcaatc
      361 cgccctctcc tgtctccgcc tggagggatg ccactgcgtc agaggcggtc tccgccgctg
      421 cgatctcctc tggcgtcagg ccggtctccg ccgccagctc tgacagggtg ggctcccgtc
      481 ccatctcggc caccagccgg cgtcgggcgc cccacactgc ggccccccgc tccttcatgc
      541 cccggctcac cttcacagtg ccgtcatccc ggagaaaacg ccggatctcc cccgcgatct
      601 tgggcaccgc ataggtggag aactgggtcc cgaaggatgg gtcaaagccc cgcactgcct
      661 tcaaaaagcc cagacagccc agctggtaca ggtcgtccgg ctccacgccc cgtccgtaat
      721 accgccgcac aatgctccag atcaggccgg cgttttcctc caacacctgc tcacaggccc
      781 ggttgtcccc ctgtctggcc gcctccagca gctctggagc cgttggcgtg ccattcatcg
      841 tccgcctatg cggggggaga tccgcttccg catggtcacc tgggtcccct tccccggggt
      901 agagcgcacc tttaccacgt ccatgaagct ctccatgatg gtaaagccca tcccggagcg
      961 ctcctcgctc ccggtggtaa acagcggggt ccgggcctgc tccacgtccg cgatgcctac
     1021 cccccagtcc cgcaccaaga tctccaggat gccgccctcc ttcagacgca gcttcagggc
     1081 caccttgccc agccggtccg ggtaggcgtg gacgatggcg ttggtcactg cctcgctcac
     1141 ggcggttttg atgtcgttga tctcctccag cgtggggtcc agctgggcgg caaagcaggc
     1201 cgccgccgcc cgggcaaacc cctcgttggc tgaccggctg ggaaattcca gcgcggcgta
     1261 attttccacc ttcatttatg tacgtcctcc tcccccgccg cggcgggctc ctcctgttca
     1321 aatcggatca gcttgtgcag gccggccgcc tgaaacacct tccacggctg cggaggcacg
     1381 ccccgcacta tcagctcgcc cttgatctgc tccatccgcc ggctgacccg gatcagtact
     1441 gcgatgcccg agctgtccgt aaagctcagg cctgacaggt ccagagtcag ccgccggggc
     1501 agagccatgt cgatctgttc atccagctct gtcatcacct ctttggcccg gtggtgatcc
     1561 agctcccccc gcaccgccgc tgtcagggtc cgcccctcgg acacgcaatc tacggtcatt
     1621 cagacttcct ccttggcccg gtcctctctg ccgggtgata tgaggagatt ataggacaag
     1681 ccccctgcaa aaccgggcat aagctgtcga ggctgggaag aaaatccatg tttgtccttg
     1741 acctcacccc tcccatctga taccatacaa gaaacaatca cgtttcacat agacgggttt
     1801 tataccatca gctatgagga gcctcctctt tccttctatt ttgaattttg agagcccctg
     1861 ttctgcttcc cgcagagcag gggctctcct gcataa
//
LOCUS       DBMDSJ010000353         1894 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13636_length_1894_cov_1.515895, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000353 DBMDSJ010000000
VERSION     DBMDSJ010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13636_length_1894_cov_1.515895"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="ACZPAQ_08065"
     CDS             complement(<1..250)
                     /locus_tag="ACZPAQ_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR0903984.1"
                     /translation="MCAALSPLAGGGGRVLAALDGPCASGKTTLAGQAARQLGCPLVH
                     MDDFFLPPARKTPARLAQPGGNVDWERVRDQVLAPLSRG"
     gene            complement(265..798)
                     /locus_tag="ACZPAQ_08070"
     CDS             complement(265..798)
                     /locus_tag="ACZPAQ_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0903985.1"
                     /translation="MSSARNKRLVVSALMLALALVLPYFTGGIPAIGNLLLPMHLPVL
                     LCGFLCGGGWGAGVGLAAPLLRSLLAGRPEFFPTAFSMAFELAAYGLISGLLYRRLPK
                     NAAGVYASLAGAMVGGRMVWGAVRALITLASPTPFGLAIFWTEAVLNAIPGILLQLVL
                     IPALVLSLQRARLSPVQ"
     gene            1200..>1894
                     /locus_tag="ACZPAQ_08075"
     CDS             1200..>1894
                     /locus_tag="ACZPAQ_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024829.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR0903986.1"
                     /translation="MPEITTPQTTETAPAPAQTTPRPPVKPNGGRKKKKMVKNLIILA
                     VVLALLAAGGFALYRFLFSTGGEEGEIFSQPAFLGSIQSKVSGSGTAKAKETAAITLN
                     AGGTVQEVLIAPGQTVTAGQPLYTIFSQAAEDAVKTAQEKVENLYKDLSDLQEDAANL
                     TIRAPFAGKLQDVKEFQIDQDVSKGTVVATLVNDKQLKLSLYFSYAYEDQISVGQSVD
                     VSIPAVMRTFTGTV"
BASE COUNT          399 a          602 c          618 g          275 t
ORIGIN      
        1 ctccccggga caggggggcc aggacctggt cccgcacccg ctcccagtcc acatttcccc
       61 ccggctgggc caggcgggcg ggggtcttgc gcgccggggg aaggaagaag tcgtccatgt
      121 ggaccagggg gcagcccaac tgccgggccg cctggccggc cagggtggtc tttcccgagg
      181 cgcaggggcc gtccagggcg gccagcaccc tcccgccgcc cccggccaga ggggacaggg
      241 ccgcgcacat ctgttccagg gccgtcattg gactggggag aggcgggcgc gctggaggga
      301 gaggaccaga gcggggatca gcaccagctg gagcaggatg ccggggatgg cgttgaggac
      361 ggcctcggtc cagaagatgg ccaggccgaa gggggtgggg gaggccaggg tgatgagggc
      421 ccgcaccgcc ccccagacca ttcgcccgcc caccatggcc cctgccagag aggcatagac
      481 gccggcggcg tttttgggca ggcggcggta gagcagcccg gagatcaggc cgtaggcggc
      541 cagctcaaag gccatggaga aggcggtggg aaagaattcg gggcggcccg ccagcagaga
      601 gcggagcagg ggggcggcca gtcccacccc ggccccccac ccgccgccgc acagaaagcc
      661 gcacagcagc accggcaggt gcatgggcag cagcagattg ccgatggcgg ggatgccgcc
      721 ggtaaaatag ggcaggacaa gggccagtgc cagcatcagg gcggagacca ccagccgttt
      781 gtttcgggca gaagacatcg gggacatacc tcctggagcc gcagaccggg ctgcggcggg
      841 atgaaaattc ctgccccaga ggcagggaac tggctttatt gtagcggaag gaagaaaatt
      901 cgtcaacaat ctcctgccag ctctgtccgg aagaaaagcg ccctcgcatc aactccagcc
      961 catcggccag aaaaattttt aaagtctgga acattttgtg cccgttcttc gtctagataa
     1021 gcagagatac tttgcccggg gcacatttct gcagaagagg acgtttctta agataatctt
     1081 aaaaaattgt aattcttatg tagttgccac ccggcggccc tgggcatata atggacatgc
     1141 tggacaggct cgcacctgac cttgtgcctg cccggcaaaa aacgtaggag gaacttgaaa
     1201 tgccagagat caccacacct cagaccaccg agaccgcgcc cgcacccgca cagacgactc
     1261 cgcggcctcc ggtcaagccc aacggcggcc ggaaaaagaa aaagatggtc aaaaacctga
     1321 tcattctggc cgttgtgctc gccctgctgg cggccggcgg ctttgcactc taccgattcc
     1381 tgttcagcac cggcggcgag gagggggaga tcttctccca gcccgccttc ctcggctcca
     1441 tccagagcaa ggtgtccggc agcggcaccg ccaaggccaa ggagaccgcc gccatcaccc
     1501 tcaacgccgg cggcaccgtg caggaggtgc tcatcgctcc cggccaaacc gtcacggcgg
     1561 gccagcccct ctacaccatc ttcagccagg ccgctgagga cgcggtgaag accgcccagg
     1621 agaaggtgga gaacctctac aaggacctgt ctgacctcca ggaggatgcc gccaacctga
     1681 cgatccgggc cccctttgcg ggtaagctcc aggacgtgaa ggagttccag atcgaccagg
     1741 acgtgtccaa gggcaccgtg gtggccaccc tggtcaacga caagcagctg aagctgtccc
     1801 tctacttcag ctacgcctat gaggaccaga tctctgtggg ccagagcgtg gacgtgtcca
     1861 tccccgcggt gatgcgcacc ttcaccggaa cagt
//
LOCUS       DBMDSJ010000354         1893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13645_length_1893_cov_2.112165, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000354 DBMDSJ010000000
VERSION     DBMDSJ010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13645_length_1893_cov_2.112165"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1893)
                     /gene="recD2"
                     /locus_tag="ACZPAQ_08080"
     CDS             complement(<1..>1893)
                     /gene="recD2"
                     /locus_tag="ACZPAQ_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR0903987.1"
                     /translation="YLSVQGRWVRHATYGAQFKAEIVERRLPQSLKEVFHYLASGAVK
                     GVGKSTARRLVEEFGEDALRVMEEEPEKLASIKGITPKRARQISESFRQQMGMRRLME
                     FLGEHQLPLTLGTALYRAYGDVALEVLRSNPYLIVGEEFGVEFSQADQLALSLGVGGE
                     DPQRLEAGLLFELAHNLNNGHSFLPRRKLIEATAILLDVSGGLLEPCLEALAGRGEVV
                     CEAVAGEDGVYLPALYDAECYVAWRVREMSRSELLPPDDLDKLIARIQRDQGIRYAPQ
                     QREAVELAACRQVMLLTGGPGTGKTTSLRGVLALFETLGLETALAAPTGRAAKRLGEL
                     CGTEASTIHRLLETGFDPHSGRLVFSHGEDDPLKADAVIVDETSMVDVPLMAALLAAL
                     RNDCRLVLVGDPDQLPSVGPGNLFGDLIRSGTVPTVRLTQIFRQAAQSAIVRNAHLVN
                     AGELPDLRKNDNDFFFLSRRDPQSAAETIVDLCRRRLPERMGIPADQIQVLSPTRRRG
                     TGTSALNQALQAALNPPSEEKGERRFGDTLFREGDRVMQVKNNYDILWREDGGAASGM
                     GVFNGDIGRIQSIDPRGECITVDFEGRIVEYSPDMLGELEPAYAVTVHKAQGSEYRAV
                     ILAALDG"
BASE COUNT          294 a          698 c          595 g          306 t
ORIGIN      
        1 cccatccagg gcagccagga tcaccgcccg gtattcgctg ccctgggcct tgtggaccgt
       61 gacggcatag gcaggctcca gctctcccag catgtccggg gaatactcta cgatccgccc
      121 ctcaaagtcc acggtgatgc actccccccg cgggtcgatg gactggatgc ggccgatgtc
      181 tccgttgaac acccccatcc cggaggccgc gcccccgtcc tccctccaga ggatgtcgta
      241 gttgtttttc acctgcatca cccggtcccc ttcccggaag agggtgtcgc cgaagcgccg
      301 ctcccccttc tcctccgagg gcggattcag cgccgcctgg agggcctggt tcagggcgga
      361 ggtgcccgtc ccccgcctcc tggtggggga gagcacctgg atctggtccg ccgggatccc
      421 catccgttcc ggcaggcgcc ggcggcacag gtccacgatg gtctccgccg cgctctgagg
      481 gtcccggcgg gagaggaaaa agaagtcgtt atcgttcttc cgcaggtcgg gcagctcccc
      541 cgcgttcacc aggtgtgcgt tgcggacgat ggcgctctgg gccgcctgcc ggaagatctg
      601 ggtgagccgc acggtgggca cagtcccgct gcggatcagg tccccgaaca gattccccgg
      661 ccccacagag ggcagctggt ccgggtctcc caccagcacc agccggcagt cattgcgcag
      721 ggcggccagc agcgccgcca tcagaggcac atccaccatg gaggtctcgt ccacgatgac
      781 ggcgtccgcc ttcagagggt cgtcctcccc gtggctgaag accagacgcc cgctgtgggg
      841 gtcaaagccg gtctccagca ggcggtggat ggtggatgcc tccgtaccgc acagctcccc
      901 cagccgcttg gccgcccggc cagtgggggc cgccagggcg gtctccagcc ccagggtctc
      961 gaacagggcc agcacccccc gcagggaggt ggtcttaccg gtacccggcc ccccggtgag
     1021 gagcatgacc tgccggcagg ctgccagctc caccgcctcc cgctgctggg gtgcgtaacg
     1081 gattccctgg tcccgctgga ttcgggcgat cagcttgtcc aggtcgtccg gcgggagcag
     1141 ctcggaccgg ctcatctccc tgacgcgcca ggccacatag cactccgcat cgtaaagggc
     1201 gggcagatat accccatcct cccccgcaac tgcctcgcag accacctcgc cccggccggc
     1261 cagggcctcc aggcagggct ccagcaggcc gccggacacg tcgagcagga tggcggtggc
     1321 ctctatcagc ttgcgccggg gcaagaagct gtgtccattg ttcaggttgt gggccagttc
     1381 aaagagcagc cccgcctcca ggcgctgggg gtcctccccc cccaccccca gagacagggc
     1441 cagctgatcc gcctgggaaa actctacacc gaactcctcc cccacgatca gataggggtt
     1501 ggagcgcagc acctccagtg ccacatcccc ataggcccgg tacagggcgg tccccagggt
     1561 cagcgggagt tggtgttccc ccaaaaactc catcagcctc cgcatcccca tctgctggcg
     1621 gaaggactcg ctgatctgcc gggcccgctt gggggtgatc cccttgatgg aggccagctt
     1681 ctccggctcc tcctccatca cccggagggc gtcctctccg aactcctcca ccaagcggcg
     1741 ggcagtggat ttgcccaccc ccttcactgc gccggaggcc aggtagtgga agacctcctt
     1801 caggctctgg ggcaggcggc gctccacgat ctccgcctta aactgggcgc cgtaggtggc
     1861 gtggcgcacc cagcgcccct gcaccgacag gta
//
LOCUS       DBMDSJ010000355         1890 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13665_length_1890_cov_2.261040, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000355 DBMDSJ010000000
VERSION     DBMDSJ010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13665_length_1890_cov_2.261040"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1890
                     /locus_tag="ACZPAQ_08085"
     CDS             <1..>1890
                     /locus_tag="ACZPAQ_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019450.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HIR0903988.1"
                     /translation="LTAPAYVEGNGRTQVQVTIGPQLGLTYQQKEGEVTFVKGEASLT
                     FRDGEKQAGSMTMDTAADLSGSVGYVPLVYLARAFGFQLIWNSGTRTAALTTPVEGFQ
                     ARDLAEIEAPAHDYSESNQLPLTGYFKASLTGEFAGREVRYYIPDTAVIRPYYHFIAV
                     PDGVNADQFLVDSGWKQVADETGQCLFVLLPDQTTYMWGDVEQEKDYIAAAKAYHSGN
                     GAYFTTFGEFYLVGYGAGAAPLEAWAAENPHLIISQAYLNGTTCGLENLRAAGEESYG
                     WKHMTDGDIRDRDGDGINERVDENGNQKILYQMADRLDQILNPDKGSEAGLLTKGDMP
                     LPTWLVNAESDDSLTYWKQVNRADSADASVTALGSVPVSGQVYGQKEDTWATEYAGRI
                     SKVVDLETAADTVSYDFTRALRDEMTDYTRYDNSISYGNGLTYRLDYTDIQVERFSSR
                     DKRAVGTVSGPDLNGNEVEAEITIQALTLDDGYVTDVLLYVPETAGTKDIPVVVVWHG
                     GSQTGHLFMDSTAWWQTAAAEGFALALPTRTNHNQGTSSDYDLPIFDKVVQYLKNDGR
                     FDMGRIYTTGQSMGSVATIALAENRASQLAAVISSNAGGPSRLSGGDAIPAGLVVGEG
                     NYADRG"
BASE COUNT          371 a          586 c          626 g          307 t
ORIGIN      
        1 ctgaccgctc ctgcctatgt ggaagggaac ggcaggaccc aggtgcaggt gaccatcggc
       61 ccccagctgg ggctgaccta ccagcagaag gagggtgagg tcacctttgt aaagggggag
      121 gccagcctga ccttccggga tggggagaag caggccggca gcatgaccat ggacaccgcc
      181 gccgatctgt ccggcagcgt aggctatgtg cccctggtct acctggcccg ggctttcggc
      241 ttccagctga tttggaacag cgggacccgg acagcggccc tgaccacgcc ggtggagggc
      301 ttccaggccc gggacctggc ggagatcgag gccccggccc acgattacag cgagagcaac
      361 cagctgcctc tgaccgggta cttcaaggca tccctcaccg gtgagtttgc cggccgtgag
      421 gtgcgctact acatccccga cacagcggtg atccgcccct attaccactt tatcgctgtg
      481 cccgacgggg tgaacgcgga ccagttcctg gtggacagcg gctggaagca ggtggcggat
      541 gagaccggcc agtgtctgtt cgtcctgctg cctgatcaga ccacctatat gtggggagat
      601 gtggagcagg agaaggacta tattgccgcc gccaaggcat atcactccgg caatggcgcc
      661 tatttcacca ccttcggtga gttctatctg gtgggctatg gagccggagc agcccccctg
      721 gaggcctggg ctgcggagaa cccccatctg atcatcagcc aggcctatct caacgggacg
      781 acctgtggcc tggagaacct ccgggcggcc ggagaggagt cctacggatg gaagcacatg
      841 accgacgggg atatccggga ccgggacggc gacggcatca acgagcgggt ggatgagaac
      901 ggcaaccaga agatcctgta tcagatggcg gaccggctgg atcagatcct gaacccggac
      961 aaggggagcg aagcaggcct tctgaccaag ggtgatatgc cccttcccac ctggctggtc
     1021 aacgcggagt ccgacgacag cctgacctac tggaagcagg tcaaccgggc ggacagcgca
     1081 gacgccagcg tgacagcact gggaagcgtt ccggtgagcg ggcaggtata cggccagaag
     1141 gaggacacct gggccaccga gtatgcgggc cggatctcca aggtggtgga tctggagacc
     1201 gccgcagaca cggtctctta tgacttcacc cgtgctctgc gggacgagat gaccgactac
     1261 acccggtatg acaactccat ctcctacggc aacggcctga cctaccggct ggattatacc
     1321 gacatccagg tggagcgctt ctcctcccgg gacaagcggg cggtggggac cgtcagcggc
     1381 ccggatctga acgggaacga ggtggaggct gagatcacca tccaggccct gaccctggac
     1441 gacggctatg tgacagacgt gctgctctat gtgccggaga cagcggggac gaaggacatc
     1501 cccgtggtgg tggtatggca cggcggctct cagaccgggc acctgttcat ggattccact
     1561 gcctggtggc agaccgccgc ggcggagggc tttgccctgg cgctgcctac ccgtaccaac
     1621 cacaaccagg gtacatcctc cgactatgac ctgcccatct tcgacaaggt ggtccagtat
     1681 ttgaagaacg acggccgctt cgatatgggc cggatctata ccaccggcca gtccatgggc
     1741 tctgtggcca ccatcgcgct ggcggagaac cgggcctccc agctggccgc ggtgatctcc
     1801 agcaatgccg gcgggcccag ccgtctctcc ggcggcgacg ccatccccgc cggactggtg
     1861 gtgggcgaag gcaactacgc cgaccggggc
//
LOCUS       DBMDSJ010000356         1889 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13677_length_1889_cov_1.763982, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000356 DBMDSJ010000000
VERSION     DBMDSJ010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13677_length_1889_cov_1.763982"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(329..1657)
                     /locus_tag="ACZPAQ_08090"
     CDS             complement(329..1657)
                     /locus_tag="ACZPAQ_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR0903989.1"
                     /translation="MELDRSKFVTPEVAAERAVRSGDWVDYGFGGGYPELMDRALAAR
                     KGRLTDVKIRGGLVIRPRIEVVECDPEQISFHYYSWHLGDYERKLQARGLVQFLPMIL
                     RSLPCLYRDRHIRCDTAFVPVSLPDDQGYCGLGISNYAWRTIFQSARTVVFEINERLP
                     RLQGVDGSHRVHLSEADYIVEGEHEPLPLRTYRDPSPTDLRIAQLVAQEIPDGAVLSL
                     GVGSVPFAVASMLADSDIKDLGCHTGTISDAFLRLFQKGKLTNSRKEVDRGYATWNLA
                     MGSQELYDWLDREPGLFRPSDVDYVHSPERIGQMSNVISINGGVELDLMGQENAESAG
                     ARQLSGIGGQLDFLEGAFRSKGGKGFICLNSTHQKKDGTLKSNIVPCIPAGSVVSAPR
                     TMIQYVATEYGVVNLTGLSLLERAQAMASIAHPSFREELLRYAGEHFGTR"
BASE COUNT          382 a          545 c          591 g          371 t
ORIGIN      
        1 cttgtcagat gccctatcct gtctgcacga gatgattgag gaacggaaga gcggggccgc
       61 cccttccccg taggggaccc atcaaagctg gtgcggcccg gaggatactt tcggttcttg
      121 cataagggag ggggcccctc ccgcctccat cttatagggg gagttgtcac ggcggatgca
      181 gtgacggatg agaggacacc agggcagtca aaaaagctgt gaggaatttt cctcacagct
      241 ttttttataa atcggtactc tgaagctttt ctgaagagaa cagtgaacta aaggggagga
      301 gcgatagact gcgggaaagg caaggagcct atctggtccc aaaatgttcg ccagcgtagc
      361 ggaggagctc ctctcggaag cttgggtggg cgatggaggc catagcctgg gcccgctcca
      421 gcagggacag gccggtgagg ttgacgaccc cgtactccgt cgccacatac tggatcatgg
      481 tacggggggc ggagaccacg ctgcccgccg ggatgcaggg gacgatgttg gacttcagtg
      541 tgccgtcctt cttttggtgt gtggaattga ggcagatgaa gccctttccg cccttagacc
      601 ggaaggcacc ctccaggaaa tccagctgcc cgccgatgcc ggagagctgc cgggcgccgg
      661 cgctctctgc gttttcctgg cccataaggt ccagttccac accgccgttg atgctgatca
      721 cattgctcat ctgcccgatg cgttccgggg agtggacata gtccacatcg ctggggcgga
      781 acagtccggg ctcccggtcc agccagtcat agagctcctg ggagcccatg gccaggttcc
      841 aggttgcata tccccggtcc acctccttgc ggctgttggt cagctttccc ttctggaaca
      901 ggcgcagaaa agcgtcgctg atggttccgg tgtgacagcc cagatccttg atgtcggagt
      961 cggccagcat acttgctacc gcaaagggga cgcttcccac ccccagggag aggaccgctc
     1021 cgtccggaat ctcctgggcc accagctggg cgatcctgag atcagtgggg ctggggtccc
     1081 ggtaggtgcg caggggcagg ggttcatgct caccctccac gatataatcc gcctccgaga
     1141 gatggacgcg gtggctgccg tccacccctt gcagccgggg caggcgctcg ttgatctcaa
     1201 agaccaccgt gcgggcggac tggaagatgg tacgccaggc gtagttggag atgcccaggc
     1261 cgcagtagcc ctggtcatcc ggcagagaga cggggacaaa tgccgtgtcg cagcggatgt
     1321 gccggtcccg gtagaggcag ggcagggagc gcaggatcat gggcaggaac tggaccaggc
     1381 cacgggcctg cagcttccgc tcgtaatccc ccaggtgcca gctgtaatag tggaaggaga
     1441 tctgctccgg gtcgcactcc accacctcga tcctggggcg gatgaccagt ccgccgcgga
     1501 tcttcacgtc tgtcagccgg cctttccggg cggccagcgc cctgtccatc aactccggat
     1561 atccgccgcc gaagccgtag tctacccagt caccggagcg gaccgccctc tccgcggcca
     1621 cctccggtgt cacaaatttg ctcctgtcaa gctccatact gaccttgtcc tccgtttctg
     1681 tctctgtgtg gtgcggcccg atagagatcc gccggtgatt tgctatgtcc catatagtac
     1741 acagaaatgc gggctctgta aatagaaatg agcataagaa atatctccgg aaacagaaag
     1801 cgctctggag agaggaggcc cgctcaggaa tacaaaatgg gatcgggaga aatttttaaa
     1861 aaggggttga caaatttgca ccaatgtat
//
LOCUS       DBMDSJ010000357         1884 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13731_length_1884_cov_1.799776, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000357 DBMDSJ010000000
VERSION     DBMDSJ010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_1884_cov_1.799776"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="ACZPAQ_08095"
     CDS             <1..1033
                     /locus_tag="ACZPAQ_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0903990.1"
                     /translation="LSTPNGALPVLLDIPIALGDGDRPLGTGPYVLTEEATGETVLTA
                     RSGWWQGRSLPFQTIHLASVGQADDLISSFDAGDITLLDADLTGTNSLGYSGSYEAWD
                     YSTTNLIYLGFNTQRGPFRSAQLRRALSRGIDRRSIAEISFAHHAVPTALPIHPDSPL
                     YHQLLSDSADYSPDDLVKGLEGQQLGSDGLVFLVNSENSSKVAAAQRIVDQLSGAGVS
                     ISLERLSWTEYLAALERGDFDLYLAEVRLTADFDLTQLVGTRGGLNYGGWSDPATDGL
                     LSAFRAAGAELRSSAAASLCSHLIQNAPIVPICFKNGSVLTQWGRLSGLDPVRDNVFH
                     GLENWVLDP"
     gene            1179..>1884
                     /locus_tag="ACZPAQ_08100"
     CDS             1179..>1884
                     /locus_tag="ACZPAQ_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982806.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HIR0903991.1"
                     /translation="MSVLRCYSQKKTGFDVEAQGLLGALRDQLGIRGLESVVILNRYD
                     ADQIDPAIYEQAKGIVFSEPQVDAVYDETFPVPEYPHRILAVEALPGQFDQRADSAAQ
                     CIQLMAGVDRPLIAYAKVYLLGGRLTEEELDKIRRYLINPVESREASMDKPATLVRTH
                     EAPDHVAVIQGFTTLDEPGLAGLLSQLGLAMDLDDLKFLQAYFRDEEKRDPTITEVRV
                     VDTYWSDHCRHTTFSTH"
BASE COUNT          330 a          659 c          567 g          328 t
ORIGIN      
        1 tctttccacc cccaacgggg cgctgccggt tctgctggac atccccattg ccctgggaga
       61 cggggaccgc cccctgggga ccggccccta tgtgctgacg gaggaggcaa caggggagac
      121 cgtcctcact gcccgctccg gctggtggca gggcaggtcc ctcccattcc agaccatcca
      181 tttagcctcg gtggggcagg cggacgacct gatctcctcc tttgacgccg gggacatcac
      241 cctgctggat gccgacctca ctggcaccaa cagcctgggc tactccggca gttatgaggc
      301 atgggattac agcaccacca atctgatcta tctgggcttc aacacccagc ggggaccttt
      361 ccgctccgcc cagctgcggc gtgccctgtc ccggggcatc gaccggcggt ccattgcgga
      421 gatctccttt gcccaccacg ccgtccccac agcgctaccc atccaccctg acagcccgct
      481 gtaccaccaa ctgctgtcag actccgcgga ctactccccc gacgatctgg tgaagggtct
      541 ggaagggcag cagctgggca gcgacgggct ggtctttctg gtgaacagcg agaacagcag
      601 caaggtggcc gccgcccagc gcatcgtgga ccagctctca ggggccggag tgtccatctc
      661 tctggagcgc ctttcctgga ccgaatatct ggccgccctg gagcgggggg actttgatct
      721 gtatctggcg gaggtgcgcc tcaccgccga cttcgatctg actcagctgg tgggaacgag
      781 gggcggcctg aattacggcg gctggtccga cccggccacc gacggcctgc tatccgcctt
      841 ccgggccgcg ggcgcggagc tgcgctcctc tgccgccgcc tcactgtgct cccacctgat
      901 ccagaacgcc cccatcgtcc ccatctgctt caaaaacggc tccgtgctga cccaatgggg
      961 acggctaagc ggactggacc cagtgcggga caacgtgttc catgggctgg agaactgggt
     1021 cctggacccc tgaccgcccg ctccagcgtg ggaggtccac cgcctttccc atcctgaact
     1081 atccacaaag gtttcccttg cgcgcccccg ctcccccggg ggcgggacac acagacggat
     1141 cgaaacataa aaaataatat atatctggag gaatggacat gagcgtactg cgctgttatt
     1201 cacagaaaaa gaccggattc gatgtggagg cccaggggct gctgggtgct ctgcgggacc
     1261 agctgggcat ccgcggcctg gagtctgtgg tcattctgaa ccgctacgac gccgaccaga
     1321 tcgaccccgc gatctatgag caggccaagg gcatcgtctt ctccgagccc caggtggatg
     1381 cggtgtatga cgagactttc cccgtccccg agtatcccca ccgcatcctg gcggtggagg
     1441 ccctgccggg gcagttcgac cagcgggccg actccgccgc ccagtgcatc cagctcatgg
     1501 ccggggtgga ccgccccctg atcgcctacg ccaaggtgta cctgctgggc ggccggctca
     1561 ctgaggagga gctggacaag atccgccgct acctcatcaa cccggtagag agccgggagg
     1621 cctccatgga caagccggcc accctggtcc gcacccacga ggcccccgac catgtggccg
     1681 tgatccaggg ctttaccacc ctggacgagc ccggactggc cggcctgctc tcccagctgg
     1741 ggctggccat ggacctggac gacctgaagt tcctccaggc ctatttccgg gatgaggaga
     1801 agcgggaccc caccatcacg gaggtccgtg tggtggatac ctactggtct gaccactgcc
     1861 gccacaccac cttttccacc caca
//
LOCUS       DBMDSJ010000358         1880 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13771_length_1880_cov_1.878021, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000358 DBMDSJ010000000
VERSION     DBMDSJ010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13771_length_1880_cov_1.878021"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="ACZPAQ_08105"
     CDS             complement(<1..355)
                     /locus_tag="ACZPAQ_08105"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HIR0903992.1"
                     /translation="MAQINVILWDVDGTLLDFSAAERRALEDCFRSFQMGPCTPELLA
                     RYSQINRTYWRRLERGELTKPQVLLGRFEEFFAQEGLNCRDIPAFNQEYQLRLGDTVV
                     FRDDAGSLVARLKGRV"
     gene            complement(373..1539)
                     /gene="thiI"
                     /locus_tag="ACZPAQ_08110"
     CDS             complement(373..1539)
                     /gene="thiI"
                     /locus_tag="ACZPAQ_08110"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HIR0903993.1"
                     /translation="MNEIILLKLGEMVLKGLNRRSFEDKLQANLHRRLRPFGQFRVYT
                     RQSATYVEPIGEGCDVEGAYEALKHVFGIVGLSLARPCGKDKDAILACAKEFLDDQLR
                     AARTFKVETKRADKTFPMTSIQLSQYVGGELHEAYDNLEVDVHHPELTVYVEVRDYAA
                     YVHTQPQPGAGGLPVGINGRAVSLLSGGIDSPVASWMIAKRGVALDMVHFFSYPYTSL
                     EAKEKVLELARLLTPWTGRLTVHVVPFTAIQEELRRSCPEELFTVIMRRFMMRIAQEV
                     ARRCGAKALVTGECLGQVASQTMDAMLVTGAVVDLPILRPVVGMDKEEIVQIARKIGT
                     FETSILPYEDCCTVFTPRHPRLRPLPGEVEFAEEKLDIQALIQAAVDGIERVQV"
     gene            complement(1677..>1880)
                     /locus_tag="ACZPAQ_08115"
     CDS             complement(1677..>1880)
                     /locus_tag="ACZPAQ_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR0903994.1"
                     /translation="LDRFCALDSECQRLMQGAFDRMGLTARSYDRILRVARTIADLDS
                     STSIQLPHLAEALQYRPPEYLRR"
BASE COUNT          327 a          608 c          629 g          316 t
ORIGIN      
        1 gtacccggcc cttcagccgg gccaccagag aaccggcgtc atcccggaac accacagtgt
       61 cccccaggcg cagctggtac tcctggttga aagcggggat gtcccggcag ttcagcccct
      121 cctgggcgaa aaactcctca aagcggccca gcaggacctg gggcttggtc agctcccccc
      181 gctccagccg ccgccagtag gtccggttga tctgggagta gcgtgccagc agctccggcg
      241 tgcaggggcc catctgaaag gagcggaagc aatcctccag agcccgccgc tccgctgccg
      301 agaagtccag cagcgtcccg tccacgtccc agaggatcac gttgatctgt gccatatcgc
      361 gcctcctgtc cctcagacct ggacccgctc gatgccgtcc accgcggctt ggatcagcgc
      421 ctggatgtcc agcttctcct cggcgaactc cacctcgccg ggcaggggcc gcagacgagg
      481 atgacggggc gtgaagacgg tgcagcagtc ctcatagggg aggatggagg tctcgaaggt
      541 gccgatcttc cgggcgatct ggacgatctc ctccttgtcc atccccacca cagggcgcag
      601 gatgggcaga tcgaccaccg cgccggtgac cagcatggcg tccatggtct ggctggccac
      661 ctggcccagg cactcaccag tgaccagggc cttagccccg caccgccggg ccacctcctg
      721 ggcgatgcgc atcatgaagc ggcgcatgat gacggtgaac agctcctccg ggcaggagcg
      781 gcgcagctcc tcctggatgg cggtgaaggg caccacgtgg acggtgagcc gcccggtcca
      841 gggggtgagc aggcgggcca gctccagcac tttctccttg gcctcgagag aggtgtaggg
      901 ataggagaag aagtggacca tgtccagggc caccccccgc tttgcgatca tccaactggc
      961 cacaggggag tcgatgcccc cggagagcag agagacggcc cggccgttga tgcccacagg
     1021 caggccgccg gcgccgggct ggggctgggt gtggacgtaa gcggcgtagt ccctcacttc
     1081 cacgtagacg gtgagttcgg gatggtggac gtccacctcc aggttgtcat aggcctcatg
     1141 gagctccccg cccacatact ggctgagctg gatggaggtc atggggaagg tcttgtccgc
     1201 ccgcttggtc tccaccttaa aggtgcgggc ggcgcggagc tggtcgtcca gaaattcctt
     1261 ggcgcaggcc aggatggcgt ccttgtcctt cccgcagggg cgggccaggg acagacccac
     1321 gatgccgaac acgtgcttca gggcctcata ggccccctcc acatcgcagc cctcgcctat
     1381 gggctccacg taggtggccg actgccgggt gtacacccgg aactggccga agggacggag
     1441 acggcggtgg aggttggcct ggagcttgtc ctcgaagctg cggcggttga gccccttgag
     1501 gaccatctca cccagcttga gcagaatgat ttcgttcatt atgtgtaaga tcctttccat
     1561 cgattcgctt cacaggcatt atatcggaaa gcgccgggga tgtccagcgg gagcggagaa
     1621 agtctcggat ctgtccgggg atgtccctcc ggcgcgtgcc ctatgcaggg cgcattctac
     1681 cgccgcaggt actccggcgg acgatattgg agggcctcgg ccaggtgggg gagctggatg
     1741 gaggtgctgc tgtccaggtc agcgatggtc cgggccaccc gcaggatgcg gtcatagctg
     1801 cgggcggtga gccccatccg gtcaaaggcc ccctgcatca gccgctggca ctccgagtcc
     1861 agggcgcaga accggtccag
//
LOCUS       DBMDSJ010000359         1875 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13814_length_1875_cov_1.514656, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000359 DBMDSJ010000000
VERSION     DBMDSJ010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13814_length_1875_cov_1.514656"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="ACZPAQ_08120"
     CDS             complement(<1..349)
                     /locus_tag="ACZPAQ_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HIR0903995.1"
                     /translation="MARPVKRRRVCGLPPVGAFRPEGGPGGPVIEVTVDEYEAVRLID
                     LLGLTQEECAAQMNVARTTVQAIYDSAREKLARMLAEGRPLAVQGGTYDLCGQAEHCC
                     GRRCRRRGGGGGRC"
     gene            complement(941..>1875)
                     /locus_tag="ACZPAQ_08125"
     CDS             complement(941..>1875)
                     /locus_tag="ACZPAQ_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR0903996.1"
                     /translation="VLGFGVAQAGGMEAVMDNARSLAGYLSLVNIHDPATGGSAPYSL
                     LTICSLLAWGLGYFGMPHILLRFMAIEEEKKLALSRRVATTWVVISMGVAVFIGVVGN
                     GMTKAGAMEQLSDAETIIVQIANLISCYGVPAALLAGVILAGILAATMSTADSQLLAA
                     SSSVSQNLAVEFFHLKISGKKSVLVARSTMVVISLIAAFLARDPDSSVFRVVSFAWAG
                     FGAAFGPVVLFALFWKRSNKWGALAGMVTGGVMVFVWKYLVAPMGGALAIYELLPAFL
                     CACVAIVMVSLLTPAPEQSILEAFECFKSKQKQK"
BASE COUNT          368 a          640 c          536 g          331 t
ORIGIN      
        1 cgcaccggcc ccctcccccg ccccggcggc ggcagcgccg gccgcagcag tgctccgcct
       61 gaccgcacag gtcgtaagtg cccccctgga ctgccagggg cctgccctcc gccagcatgc
      121 gggccagctt ctcccgggca gagtcgtaga tggcctgcac cgtggtccgg gccacattca
      181 tctgggcagc acactcctcc tgagtcagcc ccagcaggtc gatgagccgc accgcctcat
      241 attcgtccac tgtcacctcg atcactgggc cgcctggccc tccttcaggc cggaacgccc
      301 ccacaggagg cagcccgcat acccttctcc gctttaccgg ccgcgccatg ggaccgcccc
      361 ctctctttcc cctttagtat agctccattt tcgtcatatg tcaataatat tttgactgaa
      421 gcttttgtac cgcggcatgt ttggggcaga ggggcacgtg tcatgactgg gcaagtaaga
      481 cggtctatgc attccaggcc aggcgctgca actcggcgag ccggtcctac agtccgcccc
      541 ccgcagggcc cagcgggtcc gacgctctct cttacaaggg cagtctccag ggctctcttc
      601 ccagacacat tccttcctgg gttcagctgc cggatgtcct tccccactcc agggcgatcc
      661 gctcttactt tttccaaaaa actcctctaa gacccgcgct gggacaaaaa gaaaaagggc
      721 caaaggccct tttttcctga gactgtcgaa aaagcggctt tgccactttt ttgagaaagc
      781 agcagcctgc cgcgcgcggg attggtccgc cgcaagcgga caaaccccac acttgcagcc
      841 tgagaagtgt tttccccgac gcacatgccg ccggcgaaaa cagatcacaa ctggattcgc
      901 gcctgcgggc gcgaactctg cgaggctttt tcctctatcg ttatttctgc ttttgcttgg
      961 acttaaagca ctcaaaagcc tctaggatgc tctgctccgg ggcgggggtc agcaggctga
     1021 ccatcacgat ggccacgcag gcgcacagaa aagcgggcag cagctcatag atggccagcg
     1081 cgccgcccat aggagccacc agatacttcc acacgaagac catcacgccg ccggtgacca
     1141 tccctgccag tgccccccac ttgttggacc gcttccaaaa cagggcaaag agcaccaccg
     1201 gcccgaaggc ggcgccgaag cccgcccagg cgaatgagac cacccggaag acagagctgt
     1261 ccgggtctcg ggccaggaat gctgcgatca gggagatgac caccatggtg ctccgggcca
     1321 ccagcacgct cttctttccg ctgatcttca ggtggaagaa ctctaccgcc aggttctggg
     1381 agacagagga ggaggcggcc agcagctggg agtcggcagt ggacatggtg gcggccagga
     1441 tacccgccaa gatcacgccg gccagcagag cggctggaac accgtagcag ctgatcaggt
     1501 tggcgatctg tacgatgatt gtctccgcgt cggacagctg ctccatggcc cccgcttttg
     1561 tcatgccgtt gcctaccaca ccgataaaca ccgccacgcc catggagatg actacccagg
     1621 tagtggccac ccgccgggag agggccagct ttttctcctc ttcaatcgcc atgaagcgca
     1681 gcaggatgtg gggcatgccg aagtagccca ggccccaggc cagcagggag cagatggtga
     1741 gcaggctgta cggggcagag cccccggtgg ccgggtcgtg gatgttgacc agggacaggt
     1801 agcccgccag agagcgggcg ttgtccatca ccgcctccat gccgccagcc tgcgccacgc
     1861 cgaagcccag cacca
//
LOCUS       DBMDSJ010000360         1873 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13831_length_1873_cov_1.920993, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000360 DBMDSJ010000000
VERSION     DBMDSJ010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13831_length_1873_cov_1.920993"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="ACZPAQ_08130"
     CDS             complement(<1..515)
                     /locus_tag="ACZPAQ_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0903997.1"
                     /translation="MKRKHMVRAAAAVLACAALTGAALALSTGDSLISLSYLNDTYIP
                     TVVAQGTKAADEKLIQAYQAASQKLDEVNQGYGVQQGSGGSYSADLRSRDMVRGDQLK
                     LPTGSVVQLAAGAGTVVHTGAVIDVTAGQTVASGAALVSGHRYLVGEDTSAVFTVDSG
                     LAKVGVQGSFE"
     gene            complement(512..1165)
                     /locus_tag="ACZPAQ_08135"
     CDS             complement(512..1165)
                     /locus_tag="ACZPAQ_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR0903998.1"
                     /translation="MKRNTKVSTAVIRRLPRYYRHLLELQGKGVIRISSSALGKSMGL
                     TASQIRQDLFCFGEFGQQGYGYKVDLLREEIGEILGISQGHTVVVLGAGNLGRALMEN
                     FKFASNGFQLLAAFDVGESVVGTTIGGVNVYHVDQLEEYLSAHKVSVGLLTLPQRAAQ
                     GVADRLVAGGVRGIWNFTNREISVSRPDVAVENVHFSDSLLTLSYLITQREEREEET"
     gene            1362..>1873
                     /locus_tag="ACZPAQ_08140"
     CDS             1362..>1873
                     /locus_tag="ACZPAQ_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013540.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0903999.1"
                     /translation="MDQKKTGALIRVLRLEKGLTQQGLAELLGVSNRAVSKWERGLGS
                     PDLSLLPALSLQLGVDLAGLLSGGLPEPDNTGGSMKHIRFFVCPQCGDLITATGEAAV
                     SCCGRRLEPLPVQKPDEAHSLQIHPVEDEWFITSGHPMEKGHCLSFLALVTGERATVM
                     KCWPEWDLQQR"
BASE COUNT          302 a          670 c          548 g          353 t
ORIGIN      
        1 ttttcaaagc tcccctgcac ccccaccttg gccaggccgg agtccacggt gaagacggcg
       61 gaggtgtcct cccccaccag atagcggtgt ccgctcacca gggcggctcc ggatgccacc
      121 gtctggcccg cagtcacgtc gatcaccgcc ccggtgtgga caacggtgcc ggcgcccgcc
      181 gccagctgta ccaccgaacc ggtgggcagc ttcagctgat ccccccggac catgtcccgg
      241 ctgcgcaggt cggcgctgta acttccgccg ctcccctgct gcaccccgta cccctggttc
      301 acctcgtcca gcttctggct ggccgcctga taggcctgga tcagcttttc gtccgccgcc
      361 ttggtcccct gggccaccac ggtggggata taggtgtcat tgagatagct cagggagatc
      421 aggctgtccc ctgtgctcag ggccagcgcc gccccggtca aggccgcgca ggccagcacc
      481 gccgccgccg cgcgcaccat atgctttcgt ttcatgtctc ctcctcacgc tcctcccgct
      541 gggtgatgag atagctcagc gtcagcaggc tgtcggaaaa atgtacgttt tccaccgcca
      601 catccggccg ggagacgctg atctcccggt tggtgaagtt ccagatcccc cggactcccc
      661 cggccaccag ccggtcggcc accccctggg ccgccctctg gggcagggtg agcaggccca
      721 cgctgacctt gtgggccgac agatactcct ccagctggtc cacatggtac acgttcactc
      781 cgccgatggt ggtgcccacc acgctctcgc ccacgtcgaa agcggccagg agctggaagc
      841 cgttgctggc gaacttgaag ttttccatca gggcccggcc caggttgcct gcccccagta
      901 ccactacggt gtgaccctgg ctgatcccca agatctcccc gatctcctcc cgcagcaggt
      961 ccaccttata tccatagccc tgctggccga actcaccgaa gcagaacagg tcctgacgga
     1021 tctgggatgc ggtcagcccc atggacttcc ccagggcgct ggaggagatc cggatcacgc
     1081 ccttcccctg aagctccagc agatgccggt aatatcgcgg cagccggcgt atcaccgccg
     1141 tggaaacctt tgtatttctc ttcatcgtgt gatgctccat cctccgtcgg cgcccggggc
     1201 gcctgtcttt cttttcagtc taccccatcc cggggctgtt gtcaatttct aaaatcaggg
     1261 gcacaggtcc cccgccgccc gttccctgcc ccggtcaacg gtccgttgat tgcttttttc
     1321 tccgtcttct ggcataatgc cggttagaag gagggagcac gatggatcaa aaaaagaccg
     1381 gtgcgctgat ccgggtcctg cggctggaaa aagggctgac ccagcagggg ctggccgagc
     1441 tgctgggagt cagcaaccgg gccgtctcca agtgggagcg cggactgggg tccccggacc
     1501 tctccctgct gcccgccctg tccctccagc tgggcgtgga tctggccgga ctgctctccg
     1561 gcggtctccc ggaacccgac aacacaggag gaagcatgaa acacattcga ttttttgtct
     1621 gcccccagtg cggcgatctc atcaccgcca ccggggaggc cgccgtctcc tgctgcggga
     1681 ggcggctgga gcccctgccc gtccagaagc cggacgaggc ccacagcctc cagatccatc
     1741 cggtggagga cgagtggttc atcacctccg gccaccccat ggagaaaggg cactgcctgt
     1801 cctttttggc cctggtcacc ggcgagcggg ccaccgtgat gaagtgctgg cccgagtggg
     1861 acctccagca gcg
//
LOCUS       DBMDSJ010000361         1872 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13841_length_1872_cov_1.854884, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000361 DBMDSJ010000000
VERSION     DBMDSJ010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13841_length_1872_cov_1.854884"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="ACZPAQ_08145"
     CDS             <1..567
                     /locus_tag="ACZPAQ_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904000.1"
                     /translation="YIGYDRLLVVLKRRDLYREECLWLGLALALLWLILAVEWRGGAD
                     RRELWFWVASLLVVTGPMLVVSPVNARMFFSSYLFLCLAASVLFRRARAEGLRPLTRL
                     NRAAAAAGVLLVAVYACNAAVWVHRLDTARAQADGGASQITLPLVPFPQFTANEMAGK
                     GDIVYLVYHQQPWDISFTFVPYEDWAEG"
     gene            624..1745
                     /locus_tag="ACZPAQ_08150"
     CDS             624..1745
                     /locus_tag="ACZPAQ_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904001.1"
                     /translation="MKRHTVPWRKLAVLIPAALLGAAGGYFFMWALDPYGMTVLLPSL
                     ACLILSFWLQAILHEGGHLVCGLLSGYRFLSFRVGSFTLLRQNGRLVLRRFYLPGTGG
                     QCLLEPPDGDEVPFRLYNLGGGLANLLSALAAALTAFLLPFPWPVFLLIFAAAGLFLG
                     AVNLIPLSMDGLANDGFNLHLLSRSPQARRTFALQLRCHALLQRGIPLRDQPARWFQL
                     PQDLEPQDPFTAAALSFLPSRLMDQGDLSGAEAAFRTLLDVPSGLLDIQRWDLTVDLL
                     SCSLLLGRSQEELAPLRTPALLHYCKATRKFTPSTSRMEFLWALLAQRDPEAALLSWR
                     QFQRCAPRWPYPATLDTDRRLMELGLQRAQGQGFPLPPL"
BASE COUNT          228 a          689 c          524 g          431 t
ORIGIN      
        1 tacatcggct atgaccgcct tctggtcgtg ctgaagcggc gggacctgta ccgggaggaa
       61 tgtctttggc tgggcctggc tctggccctg ctctggctga tcctggcggt cgagtggcgg
      121 ggcggcgcgg acaggcggga gctctggttc tgggtggcct ccctgctggt ggtcaccggc
      181 cccatgctgg tggtctcccc ggtgaacgcc cggatgttct tctccagcta cctgtttctg
      241 tgcctggccg cgtcagtgct cttccgacgt gcccgggcag agggcctgcg gcccctcacc
      301 cgcctgaatc gggcggctgc cgccgctggg gtcctgctgg tggcagtgta tgcctgcaac
      361 gctgccgtct gggtccatcg gctggacacc gcccgcgccc aggctgacgg aggcgccagc
      421 cagatcaccc tgcccctggt cccctttccc cagttcaccg ccaatgagat ggcaggcaag
      481 ggagacatcg tctatctggt ctaccaccag cagccttggg acatctcatt tacctttgtg
      541 ccctatgagg actgggcgga gggttgagct tttctctcct atttgtattt tttcgacatg
      601 tcacaacaga aaggactgat cgtttgaagc ggcacaccgt cccctggcgg aagctggctg
      661 tccttatccc tgccgccctt ctgggggccg cagggggcta cttcttcatg tgggctctgg
      721 acccctatgg aatgactgtc ctgctgccct ccctggcctg cctgatcctc tccttctggc
      781 tccaggccat cctgcacgag ggcggtcacc tagtctgcgg cctgctctcc ggctaccgct
      841 ttctctcctt ccgggtgggc tctttcaccc tgctccgcca gaatggccgc ctggtcctgc
      901 gccgcttcta cctccccggc actggcggcc agtgcctgct ggagccccca gacggggacg
      961 aggtcccctt ccggctttat aatcttgggg gcggcttggc caatctgctt tccgccctgg
     1021 cggcggccct gaccgccttt ctcctcccct ttccctggcc ggtcttcctg ctgatctttg
     1081 cagctgccgg cctgtttctg ggggccgtca acctcatccc tctctccatg gacgggttgg
     1141 ccaacgacgg cttcaacctg cacctgctct cccgcagccc ccaggcccgc cggaccttcg
     1201 ccctccagct gcggtgccac gccctcctcc agcggggcat ccccctccgg gaccagcccg
     1261 cccgctggtt ccagctcccc caggacctgg agccccagga cccctttacc gccgccgccc
     1321 tatccttcct ccccagccgg ctcatggacc agggggacct gtccggggcg gaagccgctt
     1381 tccgaaccct gctggacgtc ccctctggcc tgctggacat ccagcggtgg gacctgacgg
     1441 tggatctgct ctcctgctcc ctgctcctgg gccgctccca ggaggagctg gcccccctcc
     1501 gcacccctgc ccttctccat tactgcaagg ctacccggaa gttcaccccc tccaccagcc
     1561 gcatggagtt cctctgggct ctgctggccc agcgggaccc ggaggccgcc ctgctgtcct
     1621 ggcgtcagtt ccagcgctgt gcgccccgct ggccctatcc cgccaccctg gacacggacc
     1681 gccgcctgat ggagcttggc ctccagcgcg cccaggggca ggggtttccc ctgcctcccc
     1741 tctgaccacc ccaacttttt cggaatattt cttccatttt cgttctgttt atgtcttttt
     1801 ttgcaatatc ttgtgaacac tttatgaatt ttagcactct ctgcttgaca gtgctaattt
     1861 cagtgctata ct
//
LOCUS       DBMDSJ010000362         1872 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13843_length_1872_cov_1.649351, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000362 DBMDSJ010000000
VERSION     DBMDSJ010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13843_length_1872_cov_1.649351"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="scfB"
                     /locus_tag="ACZPAQ_08155"
     CDS             <1..1060
                     /gene="scfB"
                     /locus_tag="ACZPAQ_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR0904002.1"
                     /translation="GTGHRMTMDFDTARRAIDWVVEKSGKRRNIEVDFFGGEPLMALD
                     TVKRTVEYARSLEEKHDKCFRFTITTNGVLLDQDTIDYINREMSNVVLSLDGRREVND
                     HMRPTVNGKGSYDVIVPKFQQLVSQRGTKDYYVRGTFTRENLDFADDVEHLASLGFRH
                     VSVEPASGPLDDPFAIKEGDLPAVEAEYEKLARQLQGRKDVNFFHFNVDLAQGPCVIK
                     RLRGCGAGCEYVAITPDGDIYPCHQFVGREEYRMGNVRSGDFDMDISGKFAELNIYTR
                     EDCRNCWARFYCSGGCSASNLLVNGDIKKPNAVACEMERKRLECAIALRAIAAGMGET
                     ENTECNNTDCSLCGSCAE"
     gene            1349..>1872
                     /locus_tag="ACZPAQ_08160"
     CDS             1349..>1872
                     /locus_tag="ACZPAQ_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904003.1"
                     /translation="MGRQGKIMLDVPSGTLAAMAVLGMAFLLGGGAGCLLGGMVNGAG
                     GSALSDYLQTYLTLAQDGTEGAPGFWAVLWEQMRFPLAALLMGFTALGVIGLPVLFAV
                     RGFLFSFSVACFCRLFGGAGLIPALFLFGLPALLWAPALFVLGAQGMLGAYGLLRRVT
                     GDSRYPLSYNTAYGV"
BASE COUNT          356 a          508 c          628 g          380 t
ORIGIN      
        1 cggtaccgga caccgcatga ccatggactt tgataccgcc aggcgggcca tcgactgggt
       61 ggtggagaag tcgggcaagc ggcggaacat tgaggtggac tttttcggcg gggaaccgct
      121 gatggcgctg gacaccgtca agcggacggt agagtacgcc cgctccctgg aggaaaagca
      181 cgacaagtgt ttccgcttta cgatcacaac caacggtgtc ctgctggacc aggacaccat
      241 cgactacatc aaccgggaga tgtctaacgt ggtgctctcc ctggacggcc ggcgggaggt
      301 caacgaccac atgcgcccca ctgtcaacgg gaaggggagc tatgacgtga tcgtccccaa
      361 gttccagcag ctggtctctc agcgggggac caaggactac tatgtgcggg gcaccttcac
      421 ccgggagaac ctggactttg ccgacgacgt ggagcacctg gcttccctgg gcttccgcca
      481 tgtttcggtg gaaccggcca gcggaccgct ggacgacccc ttcgccatca aggagggaga
      541 cctgcccgca gtggaggcag aatacgagaa gctggcccgg cagctgcagg ggaggaagga
      601 cgtgaacttc ttccacttca atgtggatct ggctcagggg ccctgcgtca tcaagcggct
      661 gcggggctgc ggggcgggct gtgagtatgt ggccatcact cctgatgggg acatctatcc
      721 ctgtcaccag tttgtgggca gggaggagta ccggatgggc aatgtccgct ccggggactt
      781 cgatatggac atctccggga agttcgcgga gctgaacatc tacacccggg aggactgccg
      841 gaactgttgg gcccgcttct attgcagcgg cggctgctcc gcctccaacc tgcttgtcaa
      901 cggcgacata aaaaagccca atgcggtagc ctgcgaaatg gagcgtaagc ggctggagtg
      961 cgccatcgcc ctgcgtgcca ttgcggccgg gatgggagag acggagaata cagaatgtaa
     1021 taatacggac tgtagtctgt gcggctcctg cgcggaatag caggaaacac aacatctgcg
     1081 gtgcctgacg gtacaaaatc actatattta gaaagaaggc gggccagcag acactggccc
     1141 gccttctttg gtcaacaaag tttacataat catgttgaca taaagtattg acggaaatga
     1201 cgaattttca caaatccttc aaaacccctt gcggaaacgg gcgccgagcg gtatattaag
     1261 gataaattca gattcggtag ggggatccaa gggttccccg cttctatttt tttatcccgc
     1321 ctcataggct gacactgagg tgagcgccat ggggagacag ggaaagatca tgctggatgt
     1381 gccgtctggg acgttggccg cgatggcggt gctgggtatg gcgttcctgc tggggggcgg
     1441 cgcgggctgc ctgctgggcg gcatggtgaa tggtgcgggc ggcagcgccc tgtccgacta
     1501 tctccagacc tatctgacgc tggcccagga cgggactgag ggagccccgg gcttttgggc
     1561 ggtgctgtgg gagcagatgc gttttcccct ggcggccctg ctgatgggat tcacggcgtt
     1621 gggggtgatc ggactgccag tgctgtttgc cgtgcggggc tttttatttt ctttttccgt
     1681 ggcctgcttc tgccgcttgt tcggcggggc ggggctgatc ccggcgctgt tcttgttcgg
     1741 gctgccggcg ctgctgtggg cgccagccct cttcgtgctg ggggcccagg ggatgctggg
     1801 ggcctacggc cttctgcggc gggtgacggg ggacagccgc tatcccctca gctataacac
     1861 cgcctacggg gt
//
LOCUS       DBMDSJ010000363         1864 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13915_length_1864_cov_2.108905, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000363 DBMDSJ010000000
VERSION     DBMDSJ010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13915_length_1864_cov_2.108905"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="ACZPAQ_08165"
     CDS             complement(<1..665)
                     /locus_tag="ACZPAQ_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR0904004.1"
                     /translation="MKIEVKKSWLSALILLALAAAAIVYLAGQFLSMREVEPIYPGPG
                     VTEIKMLSDWYPGLEGTAGDTEVYVLEGEQEGDSMLVLGGTHPNEPSGLVAAVLLIEN
                     AQPEAGTLYVIPRTNNSAFTCTDPQEGAPMRFTIDTPNGERWFRFGSRATNPIHQWPD
                     PEIYVHASSGQRLSGSETRNINRSYPGRTDGTFTEKMSYGVTQLIEQEGIDITVDLHE
                     ASP"
     gene            complement(679..825)
                     /locus_tag="ACZPAQ_08170"
     CDS             complement(679..825)
                     /locus_tag="ACZPAQ_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904005.1"
                     /translation="MLLYVYLGLVLFLLVFVIKNMVREKNLMLQIDAALVVVPLVLRL
                     LLIK"
     gene            complement(842..>1864)
                     /locus_tag="ACZPAQ_08175"
     CDS             complement(842..>1864)
                     /locus_tag="ACZPAQ_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR0904006.1"
                     /translation="NAVIVSRFHKVPAVMLILLMLIIMFPGMITGSSTAAVLSAGAIV
                     APILLMMGIPAPQTGAILAVGAIMGMAAPPVNIPAMLIAGGVDMPYIGFEGPLAFMTF
                     PCAIFTVLFVGLRYCRHLDYDKIKEGLNLEVGKQYGLKLYIPILVVIVLMVGSRALPN
                     VFPSLGMPLIFFIGSVVGLFTGKKFNAIQAVSEAVETTVPVLGKLMGVGMFIQVMTLT
                     GVRGYIVVSCFGFGLIVLYAAVCLVMPAFGAVSSYGAASVLGVPFLLALLDTGDQIVI
                     AAALSFICSMGDLMPPTALAGNYAAQIVEQKYSRVLRHCAIPFIVCILYGLVTLLVSG
                     NLSFLT"
BASE COUNT          380 a          558 c          625 g          301 t
ORIGIN      
        1 tcgggagatg cctcgtggag atccaccgtg atgtcgatgc cctcctgttc aatgagctgg
       61 gtcacgccgt agctcatttt ctcggtgaaa gtgccatcgg tacgccccgg gtaggagcgg
      121 ttgatattcc gggtctcgga gccggacagc cgctgtccgg aggaggcgtg gacatagatc
      181 tccgggtcgg gccactggtg gatggggttg gtggcccggg agccgaagcg gaaccagcgc
      241 tccccgttgg gggtgtcgat ggtaaagcgc atgggggccc cctcctgggg gtcagtgcag
      301 gtgaaggcgc tgttgttggt ccgcgggatg acgtacaggg tgcccgcctc aggctgggcg
      361 ttctcaatga gcaggacggc ggccaccagg ccggaaggct cattggggtg ggtgcccccc
      421 agcaccagca tggagtcccc ctcctgctcc ccctccagca catagacctc cgtgtctccg
      481 gcggttccct ccagaccggg ataccagtcg gacagcatct tgatctctgt cactccgggg
      541 ccagggtaga tgggctccac ctcccgcatg gagaggaact ggccggccag atacacgatg
      601 gcggcggcgg ccagagccag caggatcagg gcggataacc agctcttttt cacttcgatc
      661 ttcactctgt ccaccccctc atttgatcag cagcagccgc agcaccagcg ggaccaccac
      721 cagcgcggcg tcgatctgca gcatcaggtt cttttcccgc accatattct tgataacgaa
      781 taccagcagg aagagcacca ggcccaaata aacatataat aacatgcttt cccctcctat
      841 ctcaagtcag gaacgaaagg ttgccggaca ccagcagggt gaccaggccg tacaggatgc
      901 agacgatgaa ggggatggcg cagtgccgca gcacccggga gtacttctgt tccacgatct
      961 gggccgcata gttgcccgcc agggccgtgg gcggcatcag gtcgcccatg gagcagatga
     1021 aggacagggc ggcggcaatg acgatctggt cgccggtgtc cagcagggcc agcaggaagg
     1081 ggacgcccag cacagaggcc gcgccgtagg aggacaccgc gccaaaggcg ggcatcacca
     1141 ggcagacggc ggcatacagg acgatgaggc cgaagccgaa gcagctgacc acgatgtagc
     1201 cccgcacgcc ggtgagggtc atcacctgga tgaacatgcc cacgcccatc agcttgccca
     1261 gcacggggac ggtggtctcc acagcctccg acactgcctg gatggcgttg aacttcttgc
     1321 cggtgaacag gcccaccacg ctgccgatga agaagatcag cggcattccc agggatggga
     1381 acacgttggg gagagcccgg ctgcccacca tgaggacgat gaccaccagg atggggatgt
     1441 agagcttgag cccgtactgt ttgcccacct ccaggttcag cccctctttg atcttgtcgt
     1501 aatccaggtg ccggcagtag cgcaggccca caaagaggac ggtgaagatg gcgcagggga
     1561 aggtcatgaa ggccaggggg ccctcaaagc cgatgtaggg catatccacg ccgccggcga
     1621 tcagcatggc ggggatgttc accggggggg cggccatgcc catgatggcg cccaccgcca
     1681 ggatggcgcc ggtctggggg gccgggatgc ccatcatcag caggatgggg gccacgatag
     1741 cgccggcgga aagcaccgcg gcggtggagg agcccgtgat catgcccggg aacatgatga
     1801 tgagcatcag cagaatcagc atgaccgccg ggaccttgtg gaagcgggag acgatgacgg
     1861 cgtt
//
LOCUS       DBMDSJ010000364         1860 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13948_length_1860_cov_1.713474, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000364 DBMDSJ010000000
VERSION     DBMDSJ010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_1860_cov_1.713474"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="ACZPAQ_08180"
     CDS             complement(<1..291)
                     /locus_tag="ACZPAQ_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0904007.1"
                     /translation="MLELQNISYGVEQDGDSKNILRDISLTIDERFVAITGPNGGGKS
                     TLAKVIAGILTPTQGRILLDGEDITGLDITQRAQKGISFAFQQPVRFKGLTVK"
     gene            complement(655..>1860)
                     /gene="thiC"
                     /locus_tag="ACZPAQ_08185"
     CDS             complement(655..>1860)
                     /gene="thiC"
                     /locus_tag="ACZPAQ_08185"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR0904008.1"
                     /translation="LMPLVAAGKVAIPANRLHKCLDPEGIGSMLRTKINVNLGVSRDC
                     KDYDVEMQKVLSAVNMGAEAIMDLSSHGNTQPFRQKLTAECPVMIGTVPVYDSVIHYQ
                     RDLATLSAKDFIDVVRLHAQDGVDFVTLHCGITRKTIDQIRKHKRKMNIVSRGGSLVF
                     AWMCMTGEENPFYEFYDEILDICREYDVTISLGDACRPGCLADATDVCQIEELVRLGE
                     LTKRAWEKDVQVMVEGPGHVPMDQIAANMKVQQTICMGAPFYVLGPLVTDIAPGYDHI
                     TAAIGGAIAAMSGAAFLCYVTPAEHLALPNLDDVKQGIIASRIAAHAADIAKGIRGAR
                     DIDDKMGDARRALDWEAQWACALDPETAKAIRDDRSPEHDDTCSMCGKFCAVRSMNKA
                     LSGEYIDIL"
BASE COUNT          314 a          577 c          573 g          396 t
ORIGIN      
        1 cttcaccgtc aggcccttaa agcggacagg ctgctggaag gcaaagctga tccccttctg
       61 ggcccgctgg gtgatgtcca gcccggtgat atcctccccg tccagcagga tgcgcccctg
      121 agtgggggtc aggatgcccg caatgacctt ggccagagtg gactttcctc cgccgttggg
      181 ccctgtgata gccacgaacc gctcgtcgat ggtcaggctg atgtcccgca ggatgttctt
      241 gctgtcgccg tcctgctcca cgccatagct gatgttttgt aactctaaca tgaggttcct
      301 cccgcgtaca ggcccggacc tgctccgacc gggctccgct ttctttctat cagtttgccc
      361 cgggactccg ctcctcaaac ggcgctcagc cccttctcgc caggatctgc ccctattcta
      421 gcacgttctg ctccaaatgt ctaactgtat tttttctgaa aacagaaatt ttcctattat
      481 tttaatgggt aaaacaacga agcggcccgt ctccggcatt tccccccgta tttgtcaaga
      541 ccgccctcgt gagagggcgg tcttcagcgc tgttcagctg ttggtccggg aatgggcgcc
      601 caccctgggg ggcgcctctc cctcctgctc ccttttctga tgggagacaa tccatcagag
      661 gatgtcgata tactccccgc tgagggcctt gttcatggag cgcacagcac agaacttgcc
      721 gcacatggag caggtgtcgt catgctcggg ggagcggtcg tcccggatgg ccttggcagt
      781 ctccgggtcc agggcacagg cccactgggc ctcccagtcc agggcccggc gggcgtcgcc
      841 catcttgtcg tcgatgtccc gggcgccccg gatgcccttt gcgatatcgg cggcgtgggc
      901 agcgatcctg ctggcaatga tgccctgctt cacatcgtcc aggttgggaa gcgccaggtg
      961 ctcggctggg gtgacatagc acaggaaagc agcgccgctc atggcggcga tggccccgcc
     1021 gatggcagcg gtgatgtggt catagccggg ggcgatgtcg gtgaccaggg ggcccagcac
     1081 atagaagggg gctcccatgc agatggtctg ctgcaccttc atgttggcgg cgatctggtc
     1141 catgggcaca tggccgggcc cctccaccat cacctgcacg tccttctccc aggcccgttt
     1201 ggtcagctcg cccaggcgga ccagttcctc gatctgacat acatcggtgg cgtcagccag
     1261 gcagccgggg cggcaggcgt cgcccaggga gatggtcaca tcgtactccc ggcagatatc
     1321 caggatctcg tcgtagaact catagaaggg attctcctcc ccggtcatgc acatccatgc
     1381 gaacacaagg gaaccgcccc gtgagacgat gttcatcttc cgcttgtgct tgcggatctg
     1441 atctatggtc ttgcgggtga tgccgcagtg gagggtgacg aagtccacgc cgtcctgggc
     1501 atggaggcgg accacatcga tgaagtcctt ggcactgagg gtggccaggt cccgctggta
     1561 gtggatgacg ctgtcataga cagggacggt gccgatcatc accgggcact cggcagtgag
     1621 cttctggcgg aagggctggg tgttgccatg gctggacagg tccatgatgg cctcggcccc
     1681 catattcacc gcagagagca ccttctgcat ctccacgtcg tagtccttgc agtcccggga
     1741 gactcccagg ttgacattga tcttggtgcg gagcatggag ccgatgccct ccgggtccag
     1801 gcatttgtgc aggcggttag cagggatggc caccttgccg gccgcgacga ggggcatcag
//
LOCUS       DBMDSJ010000365         1859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13956_length_1859_cov_1.669511, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000365 DBMDSJ010000000
VERSION     DBMDSJ010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13956_length_1859_cov_1.669511"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="ACZPAQ_08190"
     CDS             complement(<1..580)
                     /locus_tag="ACZPAQ_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR0904009.1"
                     /translation="MFEAVTSSPFFGLALTLGCWCLGVELQRRTGLLICNPVLVASLL
                     VIGALTVLRVPLECYNAGGGLVKLMLGPATAVLALNIYQQRQVLREHFLPVLAGCLAG
                     SAASMGSVLLLCRLFRAEEMLAASMLPKSVTTAIALGISESGGGIPGITAAAVVITGV
                     EGAMLAPLFARCFRITDPVAEGVAIGASGHAVR"
     gene            complement(573..941)
                     /locus_tag="ACZPAQ_08195"
     CDS             complement(573..941)
                     /locus_tag="ACZPAQ_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR0904010.1"
                     /translation="MHILTELSLIFALCLAGEGIAALLPVAFPASVISMLLLMALLLT
                     GVVKERRIQTVSRFLVTNMGLFFIPSLVGTMAYVEVLRAQALPFLAVTFLTTPVVYLA
                     AAWTVQLLMRRSRKEAGRHV"
     gene            complement(1029..>1859)
                     /locus_tag="ACZPAQ_08200"
     CDS             complement(1029..>1859)
                     /locus_tag="ACZPAQ_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme domain-containing
                     protein"
                     /protein_id="HIR0904011.1"
                     /translation="GGTFTLGESEEGFQVMDNPGDAKYGMTREQLTQAFRRLKELGAE
                     EFGIHAFLASNTLSNDYYPALARILFRVAVELKEETGCHITFINLSGGVGVPYRPEQP
                     ANDIAVIGEGVRRAYEEVLVPAGMGDVAICTELGRFLLAPYGCLVTRATHEKHIYKEY
                     IGVDACAANLMRPAMYGAYHHITVMGKEDAPCDHKYDVTGALCENNDKFAVDRMLPKI
                     DMGDLLVIHDTGAHGHAMGYQYNGRLRSAEVLLCQDGSTRLIRRAETPEDYFSTAVWE
                     "
BASE COUNT          353 a          617 c          587 g          302 t
ORIGIN      
        1 atctcacggc gtggccgctg gcgcctatgg ctactccctc cgccaccggg tcggtgatgc
       61 ggaagcatct ggcaaacaga ggggccagca tggctccttc cactcccgtg atgaccacgg
      121 cggccgcggt gatgccgggg atgcctcccc cgctctccga gatgcccagc gcgatggctg
      181 tggtgacact cttaggcagc atggaggcag caagcatctc ctcggcgcgg aacagccggc
      241 acagcagcag cacggagccc atgctggcgg cgctgcccgc cagacaccct gccaacacgg
      301 ggagaaagtg ttcccgcagc acctgccgct gttgatagat attcagggcc agcaccgcgg
      361 tggccgggcc cagcatcagc tttaccagcc cgccgccggc attgtagcac tccaggggga
      421 cgcgtaatac tgtcagcgcc ccgatcacca gcagggaggc caccagcact gggttacaga
      481 tcagcagtcc tgtccgtctc tggagctcca cccccaggca ccagcacccc agggtgaggg
      541 ctaacccgaa aaacggggag gaagtcaccg cttcaaacat gacgccctgc ctcctttctg
      601 ctccgccgca tcagcagctg gaccgtccag gcggccgcca gatagaccac cggcgtagtc
      661 agaaaggtaa cggccagaaa gggcagggcc tgggccctca ggacctctac atatgccatg
      721 gtccctacta aggaggggat gaaaaacagc cccatgttgg tcaccaggaa gcgggagact
      781 gtctggatcc gccgctcctt gaccacgccg gtcaagagca gggccatgag cagcagcatg
      841 ctgatgacgc tggcggggaa ggccaccgga agcagggcgg cgatcccctc ccccgccagg
      901 cacagggcaa agatcaggct gagttcagtc aggatatgca acaaaatcac ctcaacaaga
      961 agcactcaaa tacaggaccg ccgcggcacg cgcggcggtc ctgtctctcg atctgtggtc
     1021 tggggagctc actcccacac tgcggtggaa aagtagtcct ccggggtctc cgcccggcgg
     1081 atcaggcggg tggagccgtc ctggcacagc agcacctcgg cggagcgcag ccggccgtta
     1141 tactggtagc ccatggcgtg tccatgggcg ccagtgtcgt ggatgaccag cagatcgccc
     1201 atgtcgatct tgggcagcat ccggtccacc gcaaatttgt cgttgttctc gcacagggcc
     1261 ccggtaacgt cgtacttgtg gtcgcagggg gcgtcctcct tgcccatgac ggtgatgtgg
     1321 tggtaggccc catacatggc gggccgcatc aggttggctg cgcaggcgtc caccccgatg
     1381 tactccttgt agatgtgctt ctcgtgggtg gcccgggtga ccagacagcc gtagggtgcc
     1441 agcaggaagc ggcccagctc ggtgcagatg gccacatccc ccatcccggc ggggaccagc
     1501 acctcctcat aggcgcggcg caccccctcc ccgataacgg cgatgtcgtt ggcgggctgc
     1561 tccgggcggt agggcacccc tacgccgcca gacaggttga tgaaggtgat gtgacagcct
     1621 gtctcttcct tcagctccac agccacccgg aacagaatcc gggccagggc ggggtaatag
     1681 tcgttggaca gggtgttgga ggccaggaag gcgtggatgc cgaactcctc cgcccccagc
     1741 tccttcagcc ggcggaaagc ctgggtcagc tgctcccggg tcatgccgta cttggcgtcc
     1801 cccgggttgt ccatgacctg gaagccctcc tcagactccc ccagagtgaa ggtcccgcc
//
LOCUS       DBMDSJ010000366         1858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13960_length_1858_cov_1.925441, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000366 DBMDSJ010000000
VERSION     DBMDSJ010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13960_length_1858_cov_1.925441"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(24..848)
                     /locus_tag="ACZPAQ_08205"
     CDS             complement(24..848)
                     /locus_tag="ACZPAQ_08205"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR0904012.1"
                     /translation="MDFTFQQYEESAQAIRARLGGFAPKVAMILGSGLGYLGDQVEGA
                     VTVPYGEIPYFKSSTAPGHKGRLVFGTLEGQRVAVMQGRMHHYEGYSYEEVSYAVRVL
                     RLLGCDTLIVTNAAGCVHTGWQAGDLMLITDHIKLFSQSPLRGENLPEFGVRFPDASK
                     LYTPRLQELARQTAQEQGLTLREGVYFYCYGPQYETPAEVRAARILGGDAVGMSTAPE
                     VVVAGHCGMEVLGITLLSNMAAGILDQPLSEREVLDAAEAAKEKFSGLIRACLRRM"
     gene            complement(859..1251)
                     /locus_tag="ACZPAQ_08210"
     CDS             complement(859..1251)
                     /locus_tag="ACZPAQ_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR0904013.1"
                     /translation="MDDKVKELLDRIRDTASAAADTAADTARVAGRKAGQMVDVAKLN
                     VQLFDLNGEFSDILKKLGQVMYDTHRGQGGESADVAGLLERADEVSEKIREVKARISD
                     LRQSRTCPACGASCGKEDKYCRGCGAPL"
     gene            1601..>1858
                     /locus_tag="ACZPAQ_08215"
     CDS             1601..>1858
                     /locus_tag="ACZPAQ_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014477.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR0904014.1"
                     /translation="MHSVRFLFLPMVLSLLLSGCAQTRETSSTIFAMNTVMELTAYGD
                     SGQQVLDQATERIYQLEHLLSVTDENSDISAINHGEGAWVPV"
BASE COUNT          329 a          637 c          541 g          351 t
ORIGIN      
        1 gaacaggggc gcgccggtgc ggctcacatc cgccgcagac aggcccggat cagccccgag
       61 aacttctcct tcgccgcctc ggcggcgtcc agcacctccc gctcggagag gggctggtcc
      121 aggatgccgg cggccatgtt ggacagcagg gtgatcccca gcacctccat gccgcagtgt
      181 ccggccacga cgacctccgg cgcggtggac atgcccactg cgtcaccccc caggatgcgg
      241 gcggcccgca cctccgcagg ggtctcgtac tgcgggccgt agcagtagaa gtagaccccc
      301 tcccgcaggg tgaggccctg ctcctgagcg gtctgccggg ccagctcctg caggcggggc
      361 gtatagagct tggaggcgtc tggaaaacgc actccgaatt ccggcaggtt ctccccccgc
      421 aggggtgact gggagaacag cttgatgtgg tcagtaatga gcatcaggtc ccccgcctgc
      481 cagcccgtat ggacgcagcc ggcggcgttg gtgacgatga gggtgtcgca ccccagcagc
      541 cggagcaccc ggaccgcgta ggagacctcc tcataggagt agccctcata gtggtgcatg
      601 cggccctgca tcaccgccac ccgctggccc tccagagtgc cgaagaccag ccggcccttg
      661 tgccccgggg cggtggagga tttgaaataa gggatctcgc cgtaggggac cgtcaccgcc
      721 ccctctacct ggtcccccag gtaacccagg ccggagccca ggatcatagc cactttgggc
      781 gcgaagccgc ccagccgggc gcggatggcc tgggcgctct cttcatactg ttggaatgtg
      841 aagtccatct ccgtctcctt acagaggggc gccgcagccc cggcagtatt tgtcctcttt
      901 gccgcaggag gcgccgcagg cagggcaggt ccggctctgg cgcagatcgg agatgcgggc
      961 cttcacctct ctgatctttt ccgagacctc gtccgcccgc tccagaaggc cggccacgtc
     1021 ggcactctct ccgccctggc ccctgtgggt gtcgtacatc acctgtccca gctttttcag
     1081 aatgtccgag aactctccgt tcaggtcaaa gagctggacg ttcagcttgg ccacgtctac
     1141 catctggccg gccttccgtc cggccacccg ggcggtgtcc gccgcggtgt cggcggctgc
     1201 ggaggcggtg tctctgatgc ggtccagcag ttcttttacc ttgtcatcca tagatgttct
     1261 cctcctgaca ataaaatgat accctagttt acaccaaatc gaggaaaaaa gcaacgacaa
     1321 aggggccccg ccccttgaaa agtccagcct cccggcacgc tccgccccct gccccggaga
     1381 gaaaagacgc cctctcatcc cctgcgcatg cgcggggcac ctcccctcca cggaaaaggt
     1441 atttcaggtc cacccctcag catttccgtc caggcggccc ctgctcctcc gatctatggc
     1501 tctccatatc tttttcaatt tttcatgaca aagccccggc catatgttaa aataataatc
     1561 actgtgtatg cagaacatcc cgatctttgg aggtaacgct gtgcactctg tccgattctt
     1621 gttcctgccc atggtgctgt ccctgctgct gtccggctgc gcccagacca gggagacctc
     1681 ctctaccata tttgccatga acaccgtgat ggagctcacc gcctatggtg acagcggcca
     1741 gcaggtgctg gaccaggcta cggagaggat ctaccagctg gagcatctgc tctccgttac
     1801 cgacgagaac agcgacatct ccgccatcaa ccatggggaa ggggcctggg tcccggtc
//
LOCUS       DBMDSJ010000367         1855 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_13995_length_1855_cov_1.406499, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000367 DBMDSJ010000000
VERSION     DBMDSJ010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13995_length_1855_cov_1.406499"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="ACZPAQ_08220"
     CDS             complement(<1..1228)
                     /locus_tag="ACZPAQ_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR0904015.1"
                     /translation="MPVIVLPYIPSTITVHLGLPDQWAENVTVSFPDYVKNVASSEIY
                     PTWELSAIRANVLAIISFALNRVYTEFYPSRGYNFQITNTTAYDQKFIRGRSFFQSIS
                     AVVDEIFNDYIRRQGFVEPLSAKFCNGTTSTCDGLSQWGSQEMAQAGYNSMEILRHYY
                     GDDIELVVNAPIQDITQSYPGSPLRLGSVGEAVAVVQTSLNRISQNYPAIPKIQPVTG
                     IFGEDTEYSVRQFQRIFNLASDGVVGKATWYKLVYLYVGVTRLSELISEGQTFYKVQF
                     QYPGVLREGDTGTEVQVLQYMLAVLAEFDEVIPPLTVDGVFGPSTANAVQSFQRQTDL
                     LPDGVVGEATWDALYREFASLEAVLQQDVARFPFQASELIPSLVDLTLAAVSGHSETP
                     RFGQHPGTDLRLGQSDQ"
     gene            complement(1238..1633)
                     /locus_tag="ACZPAQ_08225"
     CDS             complement(1238..1633)
                     /locus_tag="ACZPAQ_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein"
                     /protein_id="HIR0904016.1"
                     /translation="MEEEFGTLQIRVYTSRAQLPIEDATVVVTQISPTGKYHLLSVQI
                     TDREGRVQPLTIPAPPRWESTGPTQDMVPFSVCQVWAEHPGYTALRVDGVQIFSGEET
                     DQDMELIPLAEGQHSLVDSSILNTSPQNL"
BASE COUNT          362 a          507 c          623 g          363 t
ORIGIN      
        1 gttgatccga ctgccccaaa cgcagatcgg tgcctggatg ctgcccgaag cggggcgtct
       61 cactgtgtcc gctgaccgcc gccagggtca ggtccaccag cgaggggatg agctcggagg
      121 cctgaaaggg gaagcgagcc acatcctgtt ggagcacggc ttccaggctg gcgaactcac
      181 ggtacagggc gtcccaggtg gcttccccca ctacgccgtc ggggaggagg tcagtctgcc
      241 tctgaaagga ctggaccgca ttggcggtgg aggggccgaa tacgccgtcc accgtcagcg
      301 gggggatgac ctcatcaaat tcagccagta cggccagcat gtactggagg acctgaacct
      361 cggtgccggt gtccccctct cggaggacgc cagggtactg gaactggacc ttatagaaag
      421 tctgcccttc gctgatcagc tcagagagac gggtgacgcc cacatagagg tagaccagct
      481 tataccaggt ggcctttccc accacgccgt ctgacgccag gttgaagatg cgctggaact
      541 gccgcacgga gtattcggtg tcttccccga agatgccggt gacgggctgg atcttgggga
      601 tggcggggta gttctgggag atgcggttca ggctggtctg gaccacggcc accgcctccc
      661 ccacggagcc cagccgcagg gggctgccgg gataggattg ggtgatgtcc tggatggggg
      721 cgttgaccac cagctcgatg tcgtccccgt aatagtggcg gaggatctcc atggagttat
      781 agcccgcctg ggccatctcc tggctgcccc actgggagag cccgtcacag gtggaggtgg
      841 tcccgttgca gaacttggcg gagaggggct ccacaaagcc ctggcggcgg atgtagtcgt
      901 tgaagatctc gtccaccacc gcgctgatgc tctggaagaa gctccttccc cgaatgaact
      961 tctggtcata ggcggtggtg ttggtgatct ggaagttgta gccccggctg gggtagaact
     1021 cggtatagac acggttcagg gcaaaggaga tgatggccag cacattggcc cggatggcgg
     1081 agagctccca ggtgggatag atctcactgg aggctacgtt tttgacgtag tcggggaaag
     1141 agacagtcac attttctgcc cactggtcgg gcaggcccag atgcaccgtg atggtgctgg
     1201 ggatgtaagg aaggacgatg accggcatag ggacacctca cagattctga ggggaggtgt
     1261 tcaggatgga gctgtcaaca agactgtgtt gcccctcggc caaggggatg agctccatgt
     1321 cctggtcggt ctcttcgccg gagaagatct ggacaccgtc cacccgcagg gcggtgtagc
     1381 ctgggtgttc cgcccagacc tggcagacgg agaaggggac catgtcctgg gtgggaccgg
     1441 tgctctccca gcgggggggc gcagggatgg tgaggggctg gacccggccc tcacggtcgg
     1501 tgatctggac agacaacagg tgatatttcc cggtcgggct gatctgggtc accacaacag
     1561 tggcgtcctc gatggggagc tgcgcccggg aggtatagac tctgatctgc agagtcccaa
     1621 actcttcctc catatggagc ctccttccgg cgtgatggtg cgccgtttac tggaccatcc
     1681 tatgccagga ccgcggagcg ggttcccctg tggaaaaaag atgcccgccg gggcaaaaac
     1741 gctcccggtc gggcatcttt tttccacaga aaccgcatct gcggtgggtt tccctttttt
     1801 tctacacaga cataccatat aatgtggata gatttttaaa aaaggagaaa tgggc
//
LOCUS       DBMDSJ010000368         1854 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14006_length_1854_cov_1.097547, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000368 DBMDSJ010000000
VERSION     DBMDSJ010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14006_length_1854_cov_1.097547"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            471..1034
                     /locus_tag="ACZPAQ_08230"
     CDS             471..1034
                     /locus_tag="ACZPAQ_08230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0904017.1"
                     /translation="MSRLHFEEARTEEQFAALSLIHALGWRTTYAGAIPADFMAREIT
                     DDRWVPTFRENLKTNRNQLLLLYDEDIPVCCATFGPARIDAGLQAGTVCKFNSRGYEG
                     WGEIISFYTHPDHKRKGYGTKLMEEVLRRLSGQGYLQVFLFVLRENENARRFYEKMGF
                     AWDGTHEDIPFPPDTICVDLRYTRSLP"
     gene            1073..>1854
                     /locus_tag="ACZPAQ_08235"
     CDS             1073..>1854
                     /locus_tag="ACZPAQ_08235"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0904018.1"
                     /translation="MRQSNDGMLGAMPVPRLVVTMSLPIMLSMLISAVYNLVDSVYVA
                     QYSDEAFLALSYAYPIQMLLVAFCSGIGVGFSAIFSRRLGEGDQEQAASAVCHGFFLY
                     GLCWLLFLLFALTGAAPFLRLSTEDPSVARQGAQYLLICCGLSIGTCMQFLTERILQA
                     MGRPAGFMIVQGSGAILNLILDPIFIFVLDLGVAGAAAATVIGQISGALIGFWLVYRL
                     RHQLPIRLRGFRPRGEMLAEMGRVAAPAVVMQSLSSFMSLGL"
BASE COUNT          301 a          619 c          495 g          439 t
ORIGIN      
        1 cctcagccac ggtccgccgg ctctccgccg cggccgcctc ccggcgctcc tggctctcct
       61 gctctgcgcc ggctaccttt tgaatggagt ccatttccat attctcacca cccgcttccc
      121 ccatctcaac gcagcggacc tgctgtgggg gtatctgctc caatactatg tgaatttgtc
      181 caaaaaagca aggggaattt tccttttttt cttatttccc tcccatttca ttcttctttc
      241 acctctcatt ttttccaaaa aggcggatat attgtgtgcc tttttcccgt tccacccctt
      301 tttcaggact ttttgtacaa tttccacctt ctgtccagtt tttctgcctt ccggggccgg
      361 cctccccttc ggcggcgggg gcccagcggc gctttcctcc ttgccaaccg ggaattggac
      421 ggatataatc gaagacagta gagcagaaca ttctgaaagg agcgctgtcc atgtcccgcc
      481 ttcattttga agaggcccgc acagaggagc agttcgccgc cctgtccctg atccacgccc
      541 tgggctggcg gaccacctac gccggcgcca tccccgccga cttcatggcc cgggagatca
      601 ccgatgaccg ctgggtcccc acattccggg aaaatctgaa aacaaatcgc aatcaactcc
      661 ttctattata cgatgaggat attcccgtct gctgtgccac cttcggcccc gcccggatcg
      721 atgcaggtct ccaggcaggt acggtgtgca agttcaactc ccgaggatat gagggctggg
      781 gagagatcat ctccttttat acccacccag accataagag gaagggctat ggcaccaagc
      841 tcatggagga agtcctccgc cgcctgagcg gccagggcta tcttcaggtc tttctctttg
      901 tcctgcggga gaatgaaaac gcccgccgct tctacgaaaa aatggggttt gcctgggacg
      961 ggacccacga ggacatcccc ttcccgccgg acaccatctg cgtggacctg cgctacaccc
     1021 gttccctgcc atgatgccgc cccatatcgg taaaacagga ggtgagagct ctgtgcgtca
     1081 gtccaatgac ggtatgctgg gcgccatgcc ggtgccccgt ttggtggtga ctatgtccct
     1141 gcccatcatg ctgtccatgc tcatcagcgc cgtctacaat ctggtggaca gcgtctatgt
     1201 ggcccagtac agcgacgagg cctttctggc cctctcttac gcctacccca tccagatgct
     1261 gctggtggcc ttctgctctg gcatcggggt gggcttcagc gccattttct cccgccggct
     1321 gggggaggga gaccaggaac aggctgcttc tgcggtctgt cacgggtttt tcctttacgg
     1381 actctgctgg ctcctcttcc ttcttttcgc cctgacaggg gccgccccct tcctgcgcct
     1441 ctccacagag gacccctccg tcgcccggca gggggcccag tacctgctga tctgctgcgg
     1501 actgtccatc ggcacctgca tgcagttttt gacggagcgc atccttcagg ccatgggccg
     1561 ccccgccggc ttcatgatcg tgcagggctc gggtgccatc ctcaacctga tcctggaccc
     1621 tatcttcatc ttcgtcctgg accttggagt tgcgggggcc gcggcagcca cggtcatcgg
     1681 tcagatctcc ggcgcgttga tcggcttctg gctggtgtac cgcctccggc accagctccc
     1741 catccgcctg cggggcttcc gcccccgggg ggaaatgctg gctgagatgg gccgcgtcgc
     1801 cgcccccgcc gtggtgatgc agtcgctgag cagcttcatg tccctggggc tgaa
//
LOCUS       DBMDSJ010000369         1851 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14033_length_1851_cov_1.190286, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000369 DBMDSJ010000000
VERSION     DBMDSJ010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14033_length_1851_cov_1.190286"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="ACZPAQ_08240"
     CDS             complement(<1..501)
                     /locus_tag="ACZPAQ_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0904019.1"
                     /translation="MHQILIVEDDPALSQGIRLALGQEGRQFVQAGTIGQAERELAER
                     TFDLLILDLNLPDGNGLDLLSRLRDRSALPVLILTANDLEMDQVTGLELGADDYVTKP
                     FSLAVLRARVNSLLRRSRPAPAALELPPFLFDFDAMVFARDGVPLELSKTEQRLLRLL
                     VEHRGQT"
     gene            1058..>1851
                     /locus_tag="ACZPAQ_08245"
     CDS             1058..>1851
                     /locus_tag="ACZPAQ_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR0904020.1"
                     /translation="MSAKQRGVLCVFAAAVLYSIGGLCIKVIPWNGMSSNGARTAVAL
                     VVIGLYLWAIRHKPRMNRWVLLGALAVCGTNILFSIANKMTTAANAIVLQFTAPIFVM
                     VFTAIFFRRRPGKLDLVTCAAVLGGILFFFVDSLSAGGMVGNVLALISGISYAGVFMM
                     NDMPEGDAISSVFWGDVFSAVVGLPFVAMETDFSATAVVSVLILGVFQVALAYILLTI
                     GLKTTPPVTASLVSGIEPVLNPILVAVFYHEMIGPTALVGAVIVVG"
BASE COUNT          315 a          585 c          622 g          329 t
ORIGIN      
        1 ggtctgtccc cggtgctcca ccagcaggcg cagcagccgc tgctccgtct tggacagctc
       61 caacggcacg ccgtccctgg caaagaccat ggcgtcaaag tcgaacagga acggggggag
      121 ctccagcgcg gcgggggcag gacggctgcg ccggagcagg ctgttcaccc gggcgcgcag
      181 caccgccagg gagaagggct tggtcacata gtcatccgcc cccagctcca gaccggtcac
      241 ctggtccatc tccagatcgt tggccgtcag gatcagcacc ggcagggcgg agcggtcccg
      301 cagccgggag agcaggtcca ggccgttccc gtcgggcagg ttcaggtcca ggatgagcag
      361 gtcaaaggtc cgctccgcca gctcccgctc cgcctggccg atggtgccgg cctggacgaa
      421 ctggcgccct tcctggccca gtgccaggcg gatgccctgg ctgagggcag ggtcgtcctc
      481 cacgatgagg atctgatgca tgagatatca ccacttatct gttgatccgc ctccccggac
      541 cccgccgggg ggcggtgcta ttgtaccatg ggccggtccg agtgacaagg gccggggcgg
      601 ccgcccggac ccagatctgt ccggaaatcc ccgcccgccc cttgacaaag aaaacggcgt
      661 gtgctatatt gcacacattg caatgaagag gaccagtagc ggagccgccg ctgcccagag
      721 aacccccgga cggtgagagg gggagagggg cgctccggga atacgcctcc gagcagccgc
      781 ccgaaccgga caagccgcag taggggacag gccgggccgc gcccgttacc gcgccggagt
      841 ccagcactcc ataaggtcct gcccgcgagg acaggacggt ccagaggtgg taccgcattt
      901 catgccctct gtccttcggg acagggggca tttttcagag agcccgatcc tctctgtgta
      961 ttcccgcgcc gcaggggcgg gaacagcggg ggagactccg caaaatgagt tcgggcggtc
     1021 tggaaatgtg gagagagaca gaaaaggggg cggtgtcgtg tccgcaaagc agagaggcgt
     1081 cctgtgcgtc tttgccgccg cggtgctgta cagcatcggg gggctctgca tcaaagtgat
     1141 cccgtggaac gggatgtcta gcaacggagc caggaccgcg gtggccctgg tggtcatcgg
     1201 gctgtacctg tgggccattc gccacaagcc caggatgaac cgctgggtcc tgctgggcgc
     1261 tttggcggtg tgtgggacca acatcctctt ctccatcgcc aacaagatga ccacggcggc
     1321 caacgccatc gtcctccagt tcaccgcccc cattttcgtg atggtgttca ccgccatctt
     1381 cttccggcgc aggccgggaa agctggatct ggtgacctgc gcggccgtgc tgggcggcat
     1441 cctgttcttc tttgtggaca gcctttccgc tggcgggatg gtggggaacg tgctggccct
     1501 catctccggg atctcctacg ccggtgtgtt catgatgaac gacatgccgg agggggacgc
     1561 catcagctcg gtcttctggg gagatgtatt cagcgcggtg gtgggactgc cctttgtggc
     1621 catggagacc gacttctccg ccacagcggt ggtcagcgtc ctgatcctgg gggtgttcca
     1681 ggtggcactg gcctatatcc tgctcaccat cggcctgaag accactcctc cggtgacggc
     1741 cagcctggtc tccggcatcg agccggtcct caaccccatc ctggtagccg tcttttacca
     1801 tgaaatgatc ggtcccactg cgctggtggg cgcggtgatc gtggtgggca g
//
LOCUS       DBMDSJ010000370         1840 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14129_length_1840_cov_1.796435, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000370 DBMDSJ010000000
VERSION     DBMDSJ010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14129_length_1840_cov_1.796435"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(281..619)
                     /locus_tag="ACZPAQ_08250"
     CDS             complement(281..619)
                     /locus_tag="ACZPAQ_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024388.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3870 domain-containing protein"
                     /protein_id="HIR0904021.1"
                     /translation="MLDFNPGEHICVVGDARPEKTNPIYLAHEKVILILVVERATGLI
                     VDADTNMVCELTRRFLRSVFVGHSLVDDLEEIRNSLLDNYLGDSKRVLYAAARSARMK
                     FLDSTSQARE"
     gene            990..1415
                     /locus_tag="ACZPAQ_08255"
     CDS             990..1415
                     /locus_tag="ACZPAQ_08255"
                     /inference="COORDINATES: protein motif:HMM:NF039403.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6291 domain-containing protein"
                     /protein_id="HIR0904022.1"
                     /translation="MENAKRGFVFYFDNYFVVEDLPPDQRGWLLSALCVYAERVWRDP
                     SVTMEEILDHYPQMSGQTRIACGFLGAAVLRDTQRWLSQRQARALRRQQGDRPGRPPA
                     PARERSWSREQDMELTRKLAARLREEAAMAEAGGAEGQD"
     gene            complement(1643..>1840)
                     /gene="rnpB"
                     /locus_tag="ACZPAQ_08260"
     ncRNA           complement(1643..>1840)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZPAQ_08260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          332 a          589 c          557 g          362 t
ORIGIN      
        1 tcctcgccgc cctccggggc gatgcgggcg atgcgtccct cccggatgca gatgtcagcg
       61 gcatagggct gggaacgctt cccgtccacc accgtgccgc cccgcaggat cagatcatac
      121 atagggatac ctccattctt tctgctgtct cgcttgtctg ttgggatgca aaaggacgca
      181 cctgcccggc ggggcgttcc gtcccgctgc ggttcagtgc gtcctgctac tggatggcag
      241 aaaggacaag tccacttccc gccgcatagc aaaatgctga ctattcacgc gcctgactgg
      301 tgctgtccag gaacttcatc cgggcggacc gggcggcggc gtacagcacc cgcttggagt
      361 cccccagata gttgtccaaa aggctgttcc ggatctcctc caggtcgtcc accaggctgt
      421 gccccacaaa gacggagcgg aggaagcgcc gggtcagctc gcagaccata ttggtgtctg
      481 cgtccacgat caggccggtg gcccgttcca ccaccaggat gaggatcacc ttctcgtggg
      541 ccaggtagat ggggttggtc ttctccgggc gggcgtcccc caccacgcag atatgctccc
      601 ccgggttgaa atccaacata aaaaacatcc ctaagtttgt tgctgttatc aaattaccac
      661 aaaagcgggg ccttgtcaac cgccccgctc caacatccca ggaaaatcac tgtgtcagcc
      721 acagtttttt cgttgtggct gaggcgcggt ttggatggaa agatgttctt gacagcgctc
      781 ccgtcccccc gcgggggaaa aacggtcctc tcccccctct ctcctccgcc gcccggcgcg
      841 gcaccgtgac catcgctctt cctatcacta taacagagat aacaatgaca ttttctattt
      901 ctctgactgt tactgttacg gggccctaat tgcacggaaa tgtgccactt tctcccccct
      961 tttccgttag gagtaaggag ggatgcggat tggagaacgc gaaacggggt tttgttttct
     1021 actttgacaa ctactttgtg gtggaggacc tccccccgga ccagcggggg tggctgctgt
     1081 ccgccctgtg cgtctacgcc gagcgggtgt ggcgggaccc gtcggtgacc atggaggaga
     1141 tcctggacca ctacccccag atgtccggcc agaccaggat cgcctgcggc tttctggggg
     1201 cggccgtcct gcgggacacc cagcggtggc tcagccagcg gcaggcccgg gccctgcgcc
     1261 gccagcaggg ggaccgtccg ggcaggcccc cggctcccgc ccgggagcgc tcctggagcc
     1321 gggagcagga catggagctg acccggaagc tggccgcccg cctgcgggag gaggcggcca
     1381 tggcggaggc agggggagca gagggacagg actgacagga ccgcatgggc gcgcccatgc
     1441 ggtctgttat cccgggcggt gaaggtgaac tggctgccgc cggcacaggt cctgtccctg
     1501 gcggccgcga tggcccgccg gcaggtgcct gctcttccgg cgccggtcct gtgatgccgg
     1561 agaacgcgga gatccactta aaaagagcat agccaacaca caaaaaaaga gtgaaggatc
     1621 tccctccact cttttttctg aagatgcaag taagcctgta agccgggttc tgtcttttaa
     1681 gacagccatc tatctcgacg caccgttgcc ggtgcgctcc agccgcctcc ctgggacggt
     1741 cgggccgacc tgtgtgtccc tccacggcgt tgctccggat agagtttaca gcgacggaca
     1801 gttcccagcc gtcgggtgag ctcttacctc acctttccac
//
LOCUS       DBMDSJ010000371         1837 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14176_length_1837_cov_1.360599, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000371 DBMDSJ010000000
VERSION     DBMDSJ010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14176_length_1837_cov_1.360599"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="ACZPAQ_08265"
     CDS             complement(<1..386)
                     /locus_tag="ACZPAQ_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HIR0904023.1"
                     /translation="MFGEMIGTISDFMYSYLLIIMLLAVGLYFTIRTRGVQLRLLAES
                     IRVVGEKSSSRESVSAFQALMVSTASRVGTGNIVGVANAIAVGGYGAVFWMWIIALLG
                     GASAFIESTLAQIYKVRDKNGGSYGGP"
     gene            complement(548..1255)
                     /gene="sigE"
                     /locus_tag="ACZPAQ_08270"
     CDS             complement(548..1255)
                     /gene="sigE"
                     /locus_tag="ACZPAQ_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR0904024.1"
                     /translation="MAVRERAARLLVRLGLYQPEKVMYIGGSDTLPSPLTREEEGELI
                     ARLGQGDPEARARLIEHNLRLVVYIARRFENTGINIEDLISIGTIGLIKAVGTYQPAK
                     SIKLATYASRCIENEILMHLRKTSSQKTELSLDEPLNTDWDGNELLLSDVLGTENDLV
                     MRPIEADVDRQLLRQALDRLEPRERHIISLRFGLDGAREQTQKEVADRLGISQSYISR
                     LEKRIIARLKKEIVRMM"
     gene            complement(1284..>1837)
                     /locus_tag="ACZPAQ_08275"
     CDS             complement(1284..>1837)
                     /locus_tag="ACZPAQ_08275"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR0904025.1"
                     /translation="GLLGGRGLSLGGGVLYSSMDLKIVLLSAAGCYAVITLIFRGAGR
                     HTAASRELLPARLSLRERQVELTALVDTGNTLTDPVSGRPVMVAEGETLSPLFPPGTG
                     PGPEDLRDPAGGLARLGEGPLRGRLRLLPYRAVGVDRGLLLAVRVDRARVGGEELGPL
                     LVAMSPTPVSDGGSYRALIGALT"
BASE COUNT          290 a          680 c          568 g          299 t
ORIGIN      
        1 gggcctccgt agctcccgcc gttcttgtcc cgcaccttgt agatctgggc cagggtgctc
       61 tcgataaagg cggacgcccc gcccagcagg gcgatgatcc acatccagaa caccgcgccg
      121 tagccgccga cggcgatggc gttggccact cccacgatgt tgcccgtccc cacccggctg
      181 gcggtggaca ccatcagggc ctggaacgcg gacaccgact ccctggagga gctcttctcc
      241 cccaccaccc ggatggactc cgccaaaagc cgcagctgca ccccccgggt gcggatggtg
      301 aagtacagcc ccaccgccag cagcatgatg atgagcagat agctgtacat gaaatcgctg
      361 atggtcccta tcatctctcc aaacatcgtc tctctccttc tttctctctg tccgggcaga
      421 acggcgcccc cgtcagtcat ccatggcggg ggcgctgctc tgcgcggagg agattatacc
      481 acaaatccgg gaagagttcc agacgccgcc ggacttttct cgcattttgt gcaaaaagcc
      541 gggggcgtca catcatgcgg acgatctcct tcttcagccg ggcgatgatc cgcttctcca
      601 gccgggagat ataggactgg gagatgccca gccggtccgc cacctccttc tgggtctgct
      661 ctctggcccc gtccagtccg aaccggaggg agatgatgtg ccgctcccgg ggctccagcc
      721 ggtccagggc ctggcggagc agctgccggt ccacgtccgc ctcgataggg cgcatcacca
      781 ggtcgttctc cgtccccagc acgtcggaca ggagcagctc gttgccgtcc cagtcggtgt
      841 tcagcggctc gtccagggac agctccgtct tctgggagga ggtcttgcgc aggtgcatca
      901 ggatctcgtt ttcgatgcag cggctggcgt aggtggccag cttgatgctc ttggccggct
      961 gataggtccc caccgccttg atcagcccga tggtcccgat ggagatcagg tcctcgatgt
     1021 tgatccccgt gttctcgaac cggcgggcga tgtacaccac cagacgcagg ttgtgctcga
     1081 tgaggcgggc ccgggcctcc gggtccccct gccccaggcg ggcgatcagt tccccctcct
     1141 cctcccgggt cagggggctg ggcagggtgt cgctcccacc gatgtacatc accttctccg
     1201 gctggtacag ccccagccgg accaggagcc gggccgcccg ctccctcacc gccatccata
     1261 acagtctcac agcagttctc ctttcaagtc agcgcgccga tgagcgcgcg gtagctccct
     1321 ccgtccgaga ccggtgtggg agacatggcc accagcaggg ggcccagctc ctccccgccc
     1381 accctggcac ggtccacccg caccgccagc agcaggcccc ggtccacccc tacggcccgg
     1441 tagggcagca ggcgcaggcg cccccggagg gggccctccc ccagccgggc cagccccccc
     1501 gccgggtccc gcaggtcctc cggcccgggc cctgtccccg gtgggaacag gggggaaagg
     1561 gtctccccct ccgccaccat caccggccgc ccggagaccg ggtcggtcag cgtgttcccc
     1621 gtgtccacca gggcagtgag ctccacctgc cgctcccgga gggagagacg ggcggggagc
     1681 agctcccggg aggcagcggt gtggcggccc gccccccgga agatcagggt gatgaccgca
     1741 tagcaccccg ctgccgagag caggacgatc ttcaggtcca tggaggagta gagcaccccg
     1801 ccccccaggc tcagcccccg cccccccagc agtccga
//
LOCUS       DBMDSJ010000372         1829 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14253_length_1829_cov_1.365162, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000372 DBMDSJ010000000
VERSION     DBMDSJ010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14253_length_1829_cov_1.365162"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="ACZPAQ_08280"
     CDS             complement(<1..1041)
                     /locus_tag="ACZPAQ_08280"
                     /inference="COORDINATES: protein motif:HMM:NF014051.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR0904026.1"
                     /translation="MTRQDQAPRRMAAAKPNTFFGGAAILAMGILVVKVIGLFYKIPL
                     VNIIGSEGSADFNNAYNIYSVLLTISTAGLPVAVSKMVSEANALGRQNQVHKVFRLSL
                     AAFLTLGVVSFLIMYFGSEQLAGMMHDSLAAAGIRALAPAVICVGCLSAFRGYAQGHG
                     NMTPTAVSQILEALCKLVIGLGLAYWLVRAGQPSHVAAAGAITGVTVGTILALAYMIF
                     NFVSTRMREEKDTQDAPDSARRILSTLMKIAIPITISSSMVGIVTVIDSALVQGQIQK
                     VLISDPDSWALYQQVVDFAPLEAARDAWQQAVSSGAAAEAVSQLYGAVELAAENISRS
                     LYGNYSGALTIYN"
     gene            complement(1075..>1829)
                     /locus_tag="ACZPAQ_08285"
     CDS             complement(1075..>1829)
                     /locus_tag="ACZPAQ_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006573268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR0904027.1"
                     /translation="ASVHGEYTSNPPLWRWMAEYAAEHRLGMHVHVSETQSEHQASLE
                     RWGKTPIQALDGCGVWDCGRSLAAHCVYTTEEDWALMAEKGISCVHNPWSNLKLGSGV
                     APIPAMMRAGVNVALGTDGMSSHNSADLFSDIKLAAVLHNGVERDPMAVNAWAALEMA
                     TVNGARALGRDTGVIAPGKTADLILVDLSAPNLIPCHDPVEDLVFSAHGSNVEMNMAR
                     GQVIYEKGSFLTLDLERVRWEVEHYALPLIFG"
BASE COUNT          320 a          588 c          598 g          323 t
ORIGIN      
        1 gttatagatg gtcagtgcgc cgctgtaatt cccatagagg ctacggctga tattttccgc
       61 cgccagctcc accgccccgt agagctggga gaccgcctcc gccgccgcgc cggagctcac
      121 ggcctgctgc caggcgtccc gtgccgcctc caggggggcg aagtccacca cctgctggta
      181 cagcgcccag ctgtccgggt cggagatgag caccttctgg atctgtccct ggaccagggc
      241 ggagtcgatg acggtgacga tgcccaccat ggaggagctg atggtgatgg ggatggcgat
      301 cttcatcagg gtggacagga tgcgccgggc ggagtccgga gcgtcctgag tgtccttctc
      361 ctcccgcatg cgggtggaga cgaagttgaa gatcatatag gccagggcca ggatggtgcc
      421 caccgtgacg ccggtaatgg cccccgccgc cgccacatgg ctgggctggc ctgcccgcac
      481 cagccagtag gcaagcccca gtccgatgac cagcttgcac agcgcttcta ggatctggga
      541 gaccgcggtg ggcgtcatat tcccgtggcc ctgggcatag ccccggaagg cggacaggca
      601 gcccacgcag atcaccgccg gcgccagcgc gcggatgccc gccgccgcaa gggagtcgtg
      661 catcatgccg gccagctgct ctgagccaaa atacatgatc aggaaggata ccacacccag
      721 ggtcaggaag gctgccaggg acaggcggaa caccttgtgc acctggttct gccgccccag
      781 ggcgttggcc tccgacacca tcttggacac cgccaccggc aggccggcgg tggagatggt
      841 cagcagcact gagtagatgt tgtaggcgtt gttgaaatcg gcgcttccct cgctgccgat
      901 gatgttgacc agcgggatct tatagaacag gccgatgacc tttaccacca agatccccat
      961 tgccaggatg gcggctcctc caaaaaatgt attcggtttg gccgctgcca ttcggcgggg
     1021 cgcttggtct tgtcgggtca tagtgtatcc tcctcaattt gtgagactgg gtcctcaccc
     1081 gaagatcagc gggagggcgt agtgctccac ttcccagcgg acccgctcca ggtccagggt
     1141 gagaaagctc cccttctcat atatgacctg tccccgggcc atattcatct ccacattaga
     1201 gccgtgggcg gaaaagacca ggtcctccac cgggtcgtga caggggatga gattgggggc
     1261 ggagaggtcc accaggatca ggtccgccgt ctttcccggg gcgatgaccc cggtatccct
     1321 gcccagggcc cgggcgccgt tgacggtggc catctccagg gcggcccagg cattcacggc
     1381 catggggtcc cgctccaccc cgttgtgcag gacggcggcc agcttgatat cggagaagag
     1441 atcggcactg ttgtgggagg acatgccatc ggtgcccagg gccacgttga ctcccgcccg
     1501 catcatggcc gggatagggg ccaccccgga gcccagcttc aggttggacc aggggttgtg
     1561 gacacaggag atccccttct ccgccatgag agcccagtcc tcctccgtgg tgtagacgca
     1621 gtgggccgcc agtgagcggc cgcagtccca caccccgcac ccgtccaggg cctggatggg
     1681 ggtcttgccc caccgctcca ggctggcctg gtgctccgac tgggtctcag agacgtggac
     1741 gtgcatcccc agtcggtgct ctgcggcgta ctcggccatc caccgccaca gagggggatt
     1801 ggaggtgtac tccccgtgga ccgaggcgt
//
LOCUS       DBMDSJ010000373         1821 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14328_length_1821_cov_2.376744, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000373 DBMDSJ010000000
VERSION     DBMDSJ010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14328_length_1821_cov_2.376744"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="ACZPAQ_08290"
     CDS             <1..228
                     /locus_tag="ACZPAQ_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904028.1"
                     /translation="WETEGVAGTRRLQAAMELARRAAGYRREPRADRAGPVSLRPGSD
                     EPLLREHDSARTVDRIFRRDARRYDGGFALF"
     gene            243..806
                     /locus_tag="ACZPAQ_08295"
     CDS             243..806
                     /locus_tag="ACZPAQ_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR0904029.1"
                     /translation="MKLAPMRYKDYVWPHNPRVYSIDYERKMAVNKVPFGTYHLQDLG
                     RTRRVMRGEGEFAGEGAYSQFGLLANTFYDPGPGLLIHPLWQTASAYFVELRLEQEPR
                     PDYVRYSFAFWEASGLYSGAVQEVTRTGEAASGGNGGVLSHTVEQGDTLWAISARYGV
                     GMTELLARNPQIRNPNLILPGEKVTVR"
     gene            803..1753
                     /locus_tag="ACZPAQ_08300"
     CDS             803..1753
                     /locus_tag="ACZPAQ_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904030.1"
                     /translation="MTAYVTTAAGRTFQLPVPLSWELNYTAGVPSDSFWVRCPWQASQ
                     GTDPAEWVRFSAAEAGETVFSGVVDECEVNQGTEGLRLEVSGRGMAALLLDNEALGQD
                     YGTATLEDILRDHVAPYGIELAGEASLPAVSPFSVPTGSSEWAVLYEFAQYCGGVCPR
                     FDRAGRLVLEGWTEVENLAISDATPVTSLTVRDRRYGVLSQILVRDRVRQTVEQVEAT
                     DFLRMGGRCRRVMTMPGRSSYQAMRYSGQFQLDRSAAELVRMEAVVPVLFYGKPGDLV
                     RIQRSDWDRNGSWRVVEASVQADEAGGRTRLELAVPDVVI"
BASE COUNT          322 a          513 c          700 g          286 t
ORIGIN      
        1 tgggagacag agggggtggc gggcacccga cggctccagg ccgccatgga actggcccgg
       61 cgggcagcgg ggtaccgccg ggagcccaga gcagaccggg cggggccggt ctcccttcgg
      121 ccaggatcgg atgagccgct tctccgggag catgactccg cacggacggt ggaccggatc
      181 tttcgccggg atgcccggcg gtacgacggg ggctttgccc tgttctgaga ggaggatctc
      241 cattgaagct ggcgcccatg cgctacaagg actatgtgtg gccccacaac ccaagggtgt
      301 actccatcga ctatgagcgg aagatggcgg tgaacaaggt gcccttcggc acctaccatc
      361 tccaggacct gggccggacc cgccgggtga tgcggggaga gggggaattt gccggagagg
      421 gggcctattc ccagttcggc ctgctggcca acacctttta tgacccaggg ccggggctgc
      481 tgatccaccc cctctggcag acggccagtg cctattttgt ggagctgcgg ctggagcagg
      541 agcccagacc ggactatgtg cgttactcct tcgctttctg ggaggccagc ggcctgtaca
      601 gcggagcggt gcaggaggtc acccggaccg gagaggcggc atccggcggg aacggcggag
      661 tgctgagcca cacggtggag cagggggaca cactgtgggc aatctcggcc cgatacgggg
      721 tggggatgac ggagctgctg gcccgaaatc cccagatccg caatccaaac ctgatcctgc
      781 cgggagagaa ggtgacagtc cggtgacagc ctatgtgacc acggcggcag gccggacctt
      841 tcagctcccc gttccgctgt cctgggagct gaactatact gccggggtgc ccagcgacag
      901 cttttgggtg cgctgtccct ggcaggcgag ccaggggacg gacccggcgg agtgggtccg
      961 attctccgca gcggaggcgg gcgagacggt gttttccggg gtggtggacg agtgcgaggt
     1021 gaaccagggg acggagggcc tgcggctgga agtatcaggc cgggggatgg ccgccctgct
     1081 gctggacaac gaggccctgg gacaggacta cgggaccgcc accctggaag acatcctgcg
     1141 agaccatgtg gccccctatg ggatcgaatt ggcgggagag gccagcctgc cagcggtgag
     1201 ccccttctcc gtgcccactg ggagcagcga gtgggcggtg ctctatgagt tcgcacaata
     1261 ctgcggcggt gtgtgtcccc gctttgaccg ggcagggcgg ctggtgctgg aggggtggac
     1321 cgaagtggaa aatctggcca taagcgatgc cacaccggtg accagcctga cggtccgaga
     1381 ccggcggtac ggggtgctct cccagatcct ggtgcgggac agggtgcgcc agacggtgga
     1441 gcaggtggag gccactgact ttctccggat gggcggcagg tgccgccggg tgatgaccat
     1501 gccggggcgg agcagctatc aggccatgcg gtacagcgga cagttccagc tggaccgctc
     1561 cgccgcggag ctggtccgga tggaggcggt ggtgccggtc ctgttctatg gaaagccggg
     1621 ggacctggtg cggatacaga ggtccgactg ggaccgaaat ggcagctggc gggtggtgga
     1681 ggcctcagta caggcggacg aggcaggggg acggacccgg ctggagctgg cggtccccga
     1741 cgttgtgata tgaggtgagc gaaatgtgga catcagaccg gagaaaaaca cggcaggccg
     1801 gtgagagctg ggtggacaga t
//
LOCUS       DBMDSJ010000374         1818 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14355_length_1818_cov_1.499126, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000374 DBMDSJ010000000
VERSION     DBMDSJ010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14355_length_1818_cov_1.499126"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="ACZPAQ_08305"
     CDS             complement(<1..996)
                     /locus_tag="ACZPAQ_08305"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR0904031.1"
                     /translation="MPITDLLERNHKLYGGETALVEINPEIQEKHRVTWKEYELIEPA
                     STVPYRREITWGVFDEKANRVANMLLSRGVKKGDKVAILLMNCLEWLPIYFGILKAGA
                     IAVPLNFRYTAQEIEYCVQLADVDVLLFGPEFIGRVEDVAPSLSRGRLLFFVGENCPS
                     FAEDYHTATANCSSEPPKVLIQDEDDAAIYYSSGTTGFPKAILLKHQALLQSARMEAV
                     HHETCHEDVFLCIPPLYHTGAKMHWFGSLFTGSRAVLLKGNSPKAIFDAVSNEGCTIV
                     WLLVPWCQDILASLDRGELKLEDYRLSQWRLMHIGAQPVPPSLIKHWMSYFPHHQY"
     gene            1681..>1818
                     /locus_tag="ACZPAQ_08310"
     CDS             1681..>1818
                     /locus_tag="ACZPAQ_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR0904032.1"
                     /translation="MALYETILKLKDLDECCRFFDDLCTPTELRSLEQRFDVAVYLQQ
                     GL"
BASE COUNT          361 a          504 c          602 g          351 t
ORIGIN      
        1 gtactggtga tgggggaaat agctcatcca gtgcttgatg agggaggggg gcaccggctg
       61 ggcgccgatg tgcatcagcc gccactggga caggcggtag tcctccagct tcagctcacc
      121 acggtccagg gaggccaaaa tgtcctggca ccagggcacc agcagccaga cgatggtgca
      181 accctcgttg gagacggcat cgaagatggc cttgggggag ttgcccttga gcaggacggc
      241 ccggctgccg gtgaacaggg agccgaacca gtgcatcttg gcgccggtgt ggtacagggg
      301 cgggatgcag aggaatacgt cctcgtgaca ggtctcgtgg tggacggcct ccatccgggc
      361 ggactggagc agggcctggt gcttgaggag gatggccttg gggaagccgg tggtgccgga
      421 ggagtagtag atggccgcgt catcctcatc ctggatgagc accttagggg gctcactgga
      481 gcagttggcg gtggcggtgt ggtagtcctc ggcaaaggag gggcagttct ctcccacaaa
      541 gaagagcagg cggccccggg acagggaggg cgccacgtcc tccacccggc cgatgaactc
      601 cgggccgaag aggagcacat ccacgtcggc cagctggacg cagtactcga tctcctgggc
      661 ggtgtagcgg aagttcaggg ggacggcgat ggctccggcc ttcaggatgc cgaaatagat
      721 gggcagccac tccaggcagt tcatcagcag gatagccacc ttgtccccct tcttcacccc
      781 gcgggagagg agcatgttgg ccacccggtt ggccttctcg tcgaagacgc cccaggtgat
      841 ctcccggcgg taggggacgg tggatgcggg ctcgatgagc tcatattcct tccacgtgac
      901 ccggtgcttt tcctggatct ccgggttgat ctccaccagg gcggtctcgc cgccgtagag
      961 cttgtgattg cgctccagca ggtcggtaat gggcatttgc ctcggtcctt tccaaaaagg
     1021 cggcggtgcc gcctaacttc attggtttta ctcgctaaag tatcatagcg aattttttcc
     1081 cgaatgtcaa gaggaaaggt cctttctttc ccaaacgcct cctgctggcg ggatctttca
     1141 gaggcagcgt ctttggagcg aaggagggga cccctgcctg gagacacaga aaatcccgct
     1201 tgacaaatgg gaagagaggt gtataatgca aaccaacaaa ggcgcagacg gagacagcgt
     1261 agagcgcgga gagagccagt cagagagttc ccggtaggtg cgaggggcct ggttccgtag
     1321 cttgaaattt cctctcccga gccgtccgag gaacgctccc tccgggggca agtagagcgg
     1381 aaccgggtcc tcccgttaca gaggctgggt attgctggat acccattgag gccgcactcg
     1441 cgagggggcg gtaaaaagag gtggtaccac ggggcacgcc ccgtcctctg gcggcagtcg
     1501 ccagaggttt ttttgtacct tttttcaaaa aacggtcccc gccagggcgg ttttctcttt
     1561 accttcacgc tttgatgtgc taaaatcact ttaaaccatt gaaccacggg cgtccctttg
     1621 cccgcggcgc gaaaaaggag tgacactatg cccaaagcgg aacatcagag gggccgcagc
     1681 atggccctgt acgagacaat cctgaagctg aaggacctgg acgagtgctg ccgttttttt
     1741 gacgacctgt gcacccccac cgagctgcgc tccctggagc agcggttcga cgtggcggtc
     1801 tacctccagc aggggttg
//
LOCUS       DBMDSJ010000375         1815 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14386_length_1815_cov_2.050758, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000375 DBMDSJ010000000
VERSION     DBMDSJ010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14386_length_1815_cov_2.050758"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /gene="purN"
                     /locus_tag="ACZPAQ_08315"
     CDS             <1..328
                     /gene="purN"
                     /locus_tag="ACZPAQ_08315"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR0904033.1"
                     /translation="PNAVINIHPSLIPSFCGVGCYGLHVHEKALEYGVKISGATVHFV
                     TEECDAGPIILQKASEVLPGDTPEVLQRRIMEQCEWKLLPQAVSLFCQDRLKVEGRAV
                     RILDCL"
     regulatory      350..430
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            489..710
                     /locus_tag="ACZPAQ_08320"
     CDS             489..710
                     /locus_tag="ACZPAQ_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904034.1"
                     /translation="MSIQTILASGFLLLGGIFLPYWAAAPGWLSYFIPLLSLIFVLLG
                     VIPLLLCLWRDFWSWYRRTHTFTQDQDPK"
     gene            729..965
                     /locus_tag="ACZPAQ_08325"
     CDS             729..965
                     /locus_tag="ACZPAQ_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904035.1"
                     /translation="MSAKTILASGFLLLGGALLLVVSSLDYSDFYSLFGLLFTLAGAV
                     PLLIHLGSRYLTWYHKSPPSSTNEDNKEDPHENS"
     gene            952..1692
                     /locus_tag="ACZPAQ_08330"
     CDS             952..1692
                     /locus_tag="ACZPAQ_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR0904036.1"
                     /translation="MKILDINELLQAHPYPGRGIVLGRSADGQKAVIIYFIMGRSENS
                     RNRIFEATGDGIRTRAYDESKMVDPSLIIYHPVRQVGEVTVVTNGDQTDTIRDSLLAG
                     KSYIDALRTRCYEPDGPNYTPRISGVVDQDGSFCLSILKSADGDPACSHRYFYEYDAP
                     LAGEGRFIHTYQENAEPLPSFEGEPRRVSVSAPDAETLASQVWASLNEDNKVSLFVQY
                     IHLATGERETVILNKHGEQAHAASKVPN"
BASE COUNT          363 a          599 c          497 g          356 t
ORIGIN      
        1 ccccaacgcc gtcatcaata tccacccctc cctcatcccc tccttctgcg gagtgggctg
       61 ctatggcctc cacgtccacg agaaggctct ggaatacggg gtaaagatct ccggtgccac
      121 cgtccacttt gtcacggagg agtgtgacgc cggccccatc atcctgcaaa aggcatctga
      181 ggtgctgccc ggcgacacgc cggaggtcct ccagcggcgc atcatggagc agtgcgagtg
      241 gaagctgctg cctcaggccg tctccctctt ctgccaggac cggctgaagg tggagggccg
      301 ggcggtccgc atcttggact gcctgtaata cgcctccaca caaagatcaa acacgcggtg
      361 taactgacgg aagtggaatg gaccacaggg agcatcgttg tggaccatgc cgaccgtctg
      421 ggcgcacccg ttttccgaaa cgcggtgcgc cctttttgcc gctgtatgag aaagaaagga
      481 gcaccctcat gtccatccag accatccttg cctccggctt tctgctcctg ggcgggatct
      541 tcctgcccta ttgggcggct gcgcccggct ggctgagcta cttcatccct ttgctcagcc
      601 tgatttttgt cctgctgggg gtgattccgc tgctgctctg cctgtggcgt gatttttggt
      661 cctggtatcg caggacccac acatttacac aagatcagga ccccaaatga gaaagaaagg
      721 agcatctcat gtccgccaag accattctcg cctccggctt tctgcttctg ggcggagccc
      781 tgcttctggt cgtcagcagc ctggattaca gtgatttcta cagtcttttt ggcctgctgt
      841 tcacgctggc aggagcggtc cccctgctca tccacctggg gtcccgctat ctcacatggt
      901 atcacaagtc tcccccatca tccacaaatg aggacaacaa ggaggatccc catgaaaatt
      961 cttgatataa atgaactcct gcaagctcac ccctaccccg gccgtggcat cgtcctgggc
     1021 cgctccgccg atggccagaa ggcggtcatc atctacttca tcatgggccg cagcgagaac
     1081 agccgcaacc gcatctttga ggccaccggg gacggtatcc gcacccgggc ctatgacgag
     1141 agcaagatgg tagacccctc ccttatcatc taccacccgg tgcgccaggt gggggaggtg
     1201 acggtggtca ccaacggaga ccagaccgac accatccggg acagcctgct ggcgggcaag
     1261 agctacatcg acgccctgcg cacccggtgc tatgagcccg atggacccaa ctataccccc
     1321 cgcatctccg gcgtggtgga ccaggacggc tccttctgcc tgtccatcct gaaatccgcc
     1381 gacggtgacc ccgcctgcag ccaccgctac ttctatgagt acgacgcccc cctggctggg
     1441 gagggccgct tcatccacac ctaccaggag aatgccgaac ctctcccttc ctttgagggg
     1501 gagccccgcc gggtgtctgt ctccgcccct gacgctgaga ccctggccag tcaggtgtgg
     1561 gccagcctga atgaggacaa caaggtgtcc ctgtttgtgc agtatatcca cctggccacc
     1621 ggggagcgtg agaccgtcat cctcaacaag cacggagagc aggcccacgc cgcctccaaa
     1681 gtcccaaact gagaaaaaga ggtgcagatc atgacagaac tggaactgaa atacggatgt
     1741 aaccccaacc agaagcccgc ccgcatcttc atggaggagg gcgatctgcc cctgaaggtg
     1801 ctcaacggcc gtccg
//
LOCUS       DBMDSJ010000376         1811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14423_length_1811_cov_1.460234, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000376 DBMDSJ010000000
VERSION     DBMDSJ010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14423_length_1811_cov_1.460234"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="ACZPAQ_08335"
     CDS             complement(<1..607)
                     /locus_tag="ACZPAQ_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR0904037.1"
                     /translation="MAEITPMMRQYLEMKERYPDSILFFRLGDFYEMFNEDAKLVSRE
                     LDLTLTTRDRNKPPEERTPMCGVPYHSCESYIARLIAKGYKVAICEQTEDPALAKGLV
                     DRDIIRIITLGTLIDSSMLEEGKNNYIGGVYADSRALGLCLCDISTGEFYVTSFPAGA
                     EAMDHLKNELGRFSPREAVLSDGAWSLEGLPQFLRERLDCRC"
     gene            complement(724..948)
                     /locus_tag="ACZPAQ_08340"
     CDS             complement(724..948)
                     /locus_tag="ACZPAQ_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0904038.1"
                     /translation="MATFSTRLQELKETKKLLQKDAAKALDVPLRTYQRYEHGQQEPQ
                     LSTLIRMADFYGVTLDYLAGRSDQGGPEES"
     gene            1030..1305
                     /locus_tag="ACZPAQ_08345"
     CDS             1030..1305
                     /locus_tag="ACZPAQ_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904039.1"
                     /translation="MTSFWDHLYQYAQEHLVVRYLQEEPTGYGRLSIQTDARRRGLED
                     LLRGPAARSFRRLWEEQQELHSLEEQALFRAGLSMGLALGGRVRDLE"
     gene            complement(1354..>1811)
                     /locus_tag="ACZPAQ_08350"
     CDS             complement(1354..>1811)
                     /locus_tag="ACZPAQ_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HIR0904040.1"
                     /translation="RRLKELIPDIVLTSDVIVGFPGETTEEFEETLSLLEQVRFDALF
                     TFIFSPRVGTPAAQMDDPMPHQQKQANFQRLVDLQSQISAEKHAAYVGKTLRCLIDGD
                     SGDARYPLAARTPGNRLVRLDGPAELIGQFRQVRITDANTWSLFGELAE"
BASE COUNT          312 a          589 c          580 g          330 t
ORIGIN      
        1 cacagcggca gtccagccgc tcccgcagga actggggcag gccctccagg ctccaggccc
       61 cgtcggagag caccgcctcc cggggagaga agcggcccag ctcgtttttc aggtggtcca
      121 tggcctccgc ccccgccgga aaggaggtga catagaactc gccggtagag atgtcgcaca
      181 ggcacagccc cagcgcccgg gagtcggcgt acaccccgcc gatgtagttg tttttcccct
      241 cctccagcat ggaggagtcg atgagggtgc cgagggtgat gatgcggatg atgtcccggt
      301 ccaccagccc cttggccagg gccgggtcct cggtctgctc gcagatggcc accttgtagc
      361 ccttggcgat gaggcgggcg atgtagctct cgcaggagtg gtagggcacg ccgcacatgg
      421 gggtgcgctc ctccgggggc ttgttccggt cccgggtggt gagcgtcagg tccagctccc
      481 gggagaccag cttggcgtcc tcgttgaaca tctcataaaa atcccccagc cggaagaaga
      541 ggatggagtc gggataccgc tccttcatct ccaggtattg gcgcatcatg ggggtgatct
      601 cagccatatc ggcaccgcct ttcggtaaaa taaaagtaaa aagtgcaaaa tcctgtggaa
      661 agtgtggaaa agtacaacga aacagacagg aggcgcacta accgccagcg cgcctcccgg
      721 gggtcagctc tcctccggcc ccccctggtc gctccgtccc gccaggtagt ccagggtgac
      781 cccgtaaaaa tccgccatcc ggatcagggt ggacagttgg ggttcctgct gaccgtgttc
      841 atacctctga taggtgcgga ggggcacatc cagcgccttt gccgcatctt tctgcaacag
      901 tttcttggtc tctttcagtt cctgaagccg cgttgaaaac gtcgccattt acacacctcg
      961 tattgacgcg ccatttttgg catgttataa tcaaaatacg ataacccgca gaagggagcg
     1021 ctattgttta tgacttcttt ttgggaccac ttatatcaat acgcccagga acatctggtc
     1081 gtccgctatc tccaggagga gcccaccggc tacggccggc tctccatcca gacggacgcc
     1141 cgccggcggg ggctggagga cctgctgcgg ggccccgccg cccgctcctt ccgccgcctc
     1201 tgggaggagc agcaggagct gcacagcctg gaggagcagg ccctcttccg ggccgggctg
     1261 tccatgggcc tggccctggg cggccgggtc cgggacttgg agtagccccg ccgcccgtcc
     1321 ctctgcgggg cccttggggc gggggctccc ccctcactcc gccagctccc cgaacaggga
     1381 ccaggtgttg gcgtcggtga tccgcacctg ccggaactgg ccgatcagct ccgccgggcc
     1441 gtccagccgc accagccggt tgcccggcgt gcgggcggcc aggggatacc gggcgtcccc
     1501 gctgtccccg tcgatcaggc agcgcagggt cttccccaca taggccgcgt gcttctccgc
     1561 ggagatctgg ctctggaggt ccaccaggcg ctggaagttg gcctgcttct gctggtgggg
     1621 catggggtcg tccatctggg cggcgggggt ccccacccgg ggggagaaga taaaggtgaa
     1681 cagggcgtcg aaccgcacct gctccagcag ggacagggtc tcctcaaact cctccgtagt
     1741 ctctccggga aagcccacga tcacgtcgga ggtgagcacg atgtccggga tgagctcctt
     1801 cagccgccgc a
//
LOCUS       DBMDSJ010000377         1811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14429_length_1811_cov_1.093567, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000377 DBMDSJ010000000
VERSION     DBMDSJ010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14429_length_1811_cov_1.093567"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="dnaG"
                     /locus_tag="ACZPAQ_08355"
     CDS             complement(<1..776)
                     /gene="dnaG"
                     /locus_tag="ACZPAQ_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR0904041.1"
                     /translation="MPIPERFLDELIARTDIVDLVGEYVRLTKKGRNYWGLCPFHSEK
                     TPSFSVSPDKQIFKCFGCGKGGGAINFIMELDSLPFRDAVGVLAKRVNLEVPDTGGFS
                     AGMKERREKLLTINKAAARVFHKWLYAPEGAAGLAYLQRRGLSRGTLTRFGLGFAPDR
                     WDGLLTALAEQGWDKADLLDAGLAVSNKDGRIYDRFRNRVMFPIIDIRGDVIGFGGRV
                     MDDSTPKYLNSPDTPVYNKSRNVFALNIAKKSRAGRVILT"
     gene            complement(843..1805)
                     /locus_tag="ACZPAQ_08360"
     CDS             complement(843..1805)
                     /locus_tag="ACZPAQ_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR0904042.1"
                     /translation="MSSRACLAEQTRGRARPEEPCGVRTCFQVDTDRIVYCKSFRRLK
                     HKTQVFLQPEGDHYRTRMTHTLEVSRIARTIARGLALNEDLTEAIALGHDLGHTPFGH
                     AGERLLNSIVPGGFAHFRQSLRVVERLEKEGAGLNLTWEVRNGIERHTGGEDAATLEG
                     QVVRLADKIAYINHDIEDALRGGVIYPIDIPLEVSQVLGFTHGERINALVVDAISASR
                     GQDRICQSPQVGEAMAVLKEFMFANVYTNPIAKGEESKAQDMLKMLFGYYQKNPDELP
                     ADFQAIRLEEGVDRAVCDYIAGMTDPFAVSEYTRLFIPMGWGVK"
BASE COUNT          277 a          627 c          566 g          341 t
ORIGIN      
        1 tccgtcagga tcacccgccc cgccctggac ttcttggcga tgttcagggc aaatacattg
       61 cggctcttgt tgtacaccgg cgtgtccggc gagttgaggt acttgggggt ggagtcatcc
      121 atcacccggc cgccgaagcc gatcacgtcc ccccggatgt cgatgatagg gaacatgacc
      181 cggtttcgga agcggtcata gatgcggccg tccttgttgc tcaccgccag cccggcgtcc
      241 agcaggtcgg ccttgtccca cccctgctcc gccagggccg tcagcagacc gtcccagcgg
      301 tcgggggcga agcccagtcc gaaccgggtc agggtccccc ggctcagccc gcgcctctga
      361 aggtaggcca gccccgccgc cccctccggg gcgtacagcc acttgtggaa cacccgggcc
      421 gccgccttgt tgatggtcag cagcttttcc cgccgctcct tcatgccggc gctgaagccg
      481 ccggtgtccg gcacctccag gttcacccgc ttggccagca ctcccaccgc gtcccggaag
      541 ggcaggctgt ccagctccat gatgaagttg atggccccgc cgcccttccc gcagccgaag
      601 cacttgaaga tctgcttgtc cggggagacg gaaaaggagg gggtcttttc tgagtggaag
      661 gggcacagtc cccagtagtt gcggcccttt ttggtcaggc gcacatactc gcccaccagg
      721 tccacgatgt cagtccgggc gatcagctcg tccaaaaacc gctccggtat gggcattttt
      781 ctcccccttc cgctattgat ggcccgaatc ccggctcttt atccctattt ggggaaaaag
      841 gcctacttca cgccccatcc catggggatg aacagccggg tgtactcgct caccgcgaag
      901 gggtcggtca tccctgcgat atagtcgcac accgcccggt ccaccccctc ctccaggcgg
      961 atcgcctgga agtcggcggg cagctcatcg gggtttttct ggtaataccc gaagagcatc
     1021 ttcagcatgt cctgggcctt gctctcctcc ccctttgcga tggggttggt gtagacattg
     1081 gcgaacatga actccttcag caccgccatg gcctccccca cctgggggga ctgacagatg
     1141 cggtcctgcc cccggctggc ggagatggcg tccaccacca gggcgttgat ccgctccccg
     1201 tgggtaaagc ccagcacctg ggacacctcc agcgggatgt cgatggggta gatcactccg
     1261 ccccgcaggg catcctcgat gtcgtggttg atgtaggcga tcttgtcggc cagacggacc
     1321 acctgcccct ccagggtggc ggcgtcctcc ccgccggtat ggcgctcgat gccgttgcgc
     1381 acttcccagg tcaggttcag tccggctccc tccttctcca gccgctccac cacccgcagg
     1441 gactgccgga agtgggcgaa gccgccgggc acgatggagt tgagcagccg ctctcccgcg
     1501 tggccgaagg gggtgtggcc caaatcgtgg cccagggcga tggcctcggt caggtcctcg
     1561 ttcagcgcca gcccccgggc gatggtccgg gcaatgcggc tgacctccag ggtgtgggtc
     1621 atccgggtac ggtagtggtc cccctccggc tggaggaaca cctgggtctt gtgcttcagc
     1681 cgccggaagg acttgcagta gacgatgcgg tctgtgtcca cctggaagca ggtgcgcacg
     1741 ccgcagggct cctccggccg cgcccggccc cgggtctgct ccgccaggca ggcgcgggag
     1801 gacagtgtct c
//
LOCUS       DBMDSJ010000378         1807 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14470_length_1807_cov_1.535756, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000378 DBMDSJ010000000
VERSION     DBMDSJ010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14470_length_1807_cov_1.535756"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="ACZPAQ_08365"
     CDS             <1..495
                     /locus_tag="ACZPAQ_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR0904043.1"
                     /translation="VREDFPADETVDLTALQPMAAPPGDAARVVPAGETDGLRVNQVF
                     IGGCGGGSLEALRETADLWRGRTVCPYVRVMVAPATSSVYVQALEEGLLDVFLDAGAL
                     VMNQGCSACWAQSQGRCDQAEVFVSTGSINCAGWAGRAHSGICLTTVRRAAQAALSGS
                     LYGS"
     gene            509..>1807
                     /locus_tag="ACZPAQ_08370"
     CDS             509..>1807
                     /locus_tag="ACZPAQ_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012550.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR0904044.1"
                     /translation="MEQTFCSRIWRLSDNIDTDIIIMSKYLAKPSLREMVPHLFEPLR
                     PELAARLRPGDAIVAGENFGCGSSREMAASVLKTAGIRCIIAKSFAKIFFRNAINNGL
                     LLIECRDLYDRCREGDTVKVEVGRQIICNGTAYPVPGMSTEVLDILRYGGLVSRMRAL
                     DKSLPAAPAPRDTLRDVHRPGHTLAEQLLIHNTGAQDLRPGDVVTVRVDEAVLHDIYT
                     PYIFQQFRDMGFYHVYDPDRISVMVDHLYPTCLDDDPRCFRYSRRFQDEYGVKRLYVA
                     DGISHQLALEERLAAPGKIIFGTDSHTTTYGAVGAFASGVGYTEMASIIGCGQLWVRV
                     PSAIKVVVNGTLPKGVFPKDIILRVLGDLTAAGAIYKSLEFTGSTIQQLSISGRAAIA
                     NMAVECGAKAALFAPDEKTAAFCGVDLADCDWLRFDGDARY"
BASE COUNT          298 a          591 c          587 g          331 t
ORIGIN      
        1 gtgcgggagg acttcccggc agatgagacg gtggatctga ctgcccttca gcccatggca
       61 gcgccgcccg gggacgccgc gcgggtcgtt cccgccggag aaaccgacgg actgcgggtg
      121 aatcaggtgt tcatcggcgg ctgcggcggc gggagcctgg aggccctgcg ggagacagcg
      181 gacctctggc ggggccggac cgtatgcccc tatgtgcggg tcatggtggc gcccgccact
      241 tcctctgtct atgtccaggc actagaggaa gggcttctgg atgtttttct ggatgccggg
      301 gcgttggtga tgaaccaggg ctgttccgcc tgctgggccc agagccaggg gcggtgcgat
      361 caggcggagg tgttcgtctc cactggctcc atcaactgtg ccggctgggc gggcagagcc
      421 cacagcggca tctgcctgac cacggtccgc cgggccgccc aggcggcgct gtccggcagt
      481 ctctatggtt cctgagagga ggtccgccat ggaacagacg ttttgcagcc gcatctggcg
      541 gctgagcgac aatatcgata ctgatatcat catcatgagc aagtatctgg ccaagccctc
      601 cctccgggag atggtccccc atctgttcga gcccctgcgg ccagagctgg ccgcccggct
      661 gcggcccggg gatgccatcg tggccgggga gaacttcggc tgcggctcct cccgggagat
      721 ggctgcctcc gtcctcaaaa ccgcgggcat ccgctgcatc atcgccaaga gctttgccaa
      781 gatctttttc cgcaatgcca tcaacaacgg cctgctgctg atcgagtgcc gggacctgta
      841 tgaccgctgc cgggaaggcg acactgtgaa ggtggaggta ggccggcaga tcatctgcaa
      901 cggcacggcc taccctgtcc ccggtatgag cacggaggta ctggatatcc tccgttacgg
      961 cggcctggtg tcccggatgc gggcgctgga caaatctctc ccggccgctc ccgctccacg
     1021 ggacacgctg cgggacgtcc accgtccagg ccataccctg gcggagcagc tcctcatcca
     1081 caacaccggc gcacaggacc tacgccccgg agacgtggtg acggtccggg tggacgaggc
     1141 ggtcctccat gatatctaca ccccttatat cttccagcag ttccgggaca tgggattcta
     1201 tcatgtgtat gacccggacc ggatctccgt catggtggac catctgtacc ccacctgtct
     1261 ggatgatgac ccccgatgct tccggtacag ccgccgcttc caggacgagt atggcgtgaa
     1321 gcgcctctat gtggcggacg gcatcagcca tcagctggcg ctggaggagc gtctggccgc
     1381 ccccggcaag atcatcttcg gcacggacag ccacaccacc acctacggtg ccgtgggcgc
     1441 cttcgcctcc ggcgtcggct atacggagat ggcctccatc atcggctgcg ggcagctgtg
     1501 ggtccgggtc ccctccgcca tcaaggtggt ggtgaacggg accctgccca aaggtgtctt
     1561 tcccaaggac atcatcctgc gggtactggg agacctgact gccgccggtg ccatctacaa
     1621 gtccctggaa ttcacaggct ctaccattca gcagctcagc atctccggcc gggccgccat
     1681 cgccaacatg gcagtggagt gcggggccaa ggcggccctg ttcgccccgg acgaaaagac
     1741 cgccgccttc tgcggcgtgg acctggcgga ctgtgactgg cttcgctttg acggggatgc
     1801 ccggtac
//
LOCUS       DBMDSJ010000379         1804 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14512_length_1804_cov_1.385790, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000379 DBMDSJ010000000
VERSION     DBMDSJ010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14512_length_1804_cov_1.385790"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="ACZPAQ_08375"
     CDS             <1..1040
                     /locus_tag="ACZPAQ_08375"
                     /inference="COORDINATES: protein motif:HMM:NF027890.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR0904045.1"
                     /translation="GSFEGRVEKINKVSYISPEGAVHFEAVVVFDNPGTLTAGMDASA
                     MLTAGDGTQIYPYQNGQTEFYETRTIEAKANGPVVGMGNLLDHANVEAGEALLYLGSS
                     TIDSDIRAKQSEIEEAQTALDEASKALADFNAVAPIDGTVTSCTLSEGAEVKSGDTVV
                     IISNTTTMLVTITVDDRNISFIKPGDYVDLDWNGTTYQGVVTAIDMGKAESGSGMTNY
                     PVTLTVENYDGSLMDGAWLQYSFVTSESSDCILVPTSAVKYVSDADGNRQAVVFVKRD
                     ARPDDVPELDLPQVEPGQQRQFPSEEDGFYPVIVTTGISDTENVEITSGVQEGDEVFV
                     NFMVTDYSSGY"
     gene            1054..1749
                     /locus_tag="ACZPAQ_08380"
     CDS             1054..1749
                     /locus_tag="ACZPAQ_08380"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0904046.1"
                     /translation="MLIEIKDLYKIYNEGKESEVRALDGVTLHIDRGEFVAIVGQSGS
                     GKSTLMNVLGCLDIPTYGEYHLNGTDVTSLSDKQLAHIRNREIGFIFQGYNLIQELDA
                     LENVTLPLIYQGVSVWDREDIAMEALEKVGMAERAHHRPSEMSGGQQQRVAIARAIAT
                     HPPIIMADEPTGALDSRTGRHVLEILRGLYREGTTILLITHDDGIAATARRVVRLSDG
                     KIIADHAQEVDWE"
BASE COUNT          368 a          579 c          557 g          300 t
ORIGIN      
        1 tgggctcctt tgagggacgg gtggagaaga tcaacaaggt cagctacata tccccggaag
       61 gggccgtcca ctttgaggcc gtagtggtct ttgacaaccc cggcaccctc accgccggga
      121 tggacgcctc cgccatgctc accgccggcg atggcaccca gatctacccc taccagaatg
      181 ggcagaccga gttctatgag acccgcacca tcgaggccaa ggccaacggc cccgtggtgg
      241 gcatgggcaa cctgctcgac cacgccaatg tggaggcggg agaggccctg ctctacctgg
      301 ggtcctccac cattgactct gacatccggg ccaagcagtc agagatcgag gaggcccaga
      361 ctgcgctgga tgaggcctcc aaggccctgg cggatttcaa cgctgtggcc ccgatcgacg
      421 ggaccgtcac ctcctgcacc ctctccgagg gggccgaggt gaagagcggc gacacggtgg
      481 tcatcatctc caacaccacc accatgctgg tcaccatcac ggtggacgac cggaacatct
      541 ccttcatcaa gcccggggac tacgtggatc tggactggaa cggcaccacc tatcagggcg
      601 tggtcaccgc catcgacatg ggaaaggctg agagcggcag cggcatgacc aactaccccg
      661 tcaccctgac ggtggagaac tacgacggct ccctgatgga cggggcctgg ctgcagtact
      721 cctttgttac cagcgagtcc agtgactgca tcctggtgcc caccagcgcg gtgaaatatg
      781 tctccgacgc ggatggaaac cgtcaggcgg tggtcttcgt caagcgggac gcccggccgg
      841 acgatgtgcc ggagctggac ctgccccagg tcgagcccgg ccagcagcgc cagttcccct
      901 cggaggagga cggcttctac cccgtcatcg tcaccaccgg catctcggac acagaaaacg
      961 tggagatcac ctccggcgtt caggaggggg acgaggtctt cgtcaacttc atggtcaccg
     1021 attacagctc cggctattga tcgaggtgct ctcatgctga tcgagatcaa agacctttac
     1081 aaaatttata acgagggcaa ggagagtgag gtccgggctc tggacggagt cacgctccac
     1141 atcgaccggg gggagttcgt ggccatcgtg ggccagtcgg gctcgggcaa gtccaccctg
     1201 atgaatgtgc tgggctgcct ggacatcccc acctatgggg agtaccacct gaacggcacc
     1261 gacgtcacct ccctgtccga caagcagctg gcccacatcc gcaacaggga gatcggcttc
     1321 atcttccagg ggtataacct gatccaggag ctggatgccc tggagaacgt caccctgccc
     1381 ctgatctatc agggggtgtc cgtgtgggac cgggaggaca tcgccatgga ggcgctggaa
     1441 aaggtgggca tggcggagcg tgcccaccac cggcccagtg agatgtccgg cggccagcag
     1501 cagcgggtgg ccatcgcccg ggccatcgcc acccatcccc ccatcatcat ggcggacgag
     1561 cccaccggcg ctctggactc caggaccggc cgacacgtgc tggagatcct ccggggcctc
     1621 taccgggagg gcaccaccat tcttctcatc acccatgacg atggcatcgc cgccaccgcc
     1681 cggcgggtgg tgcggctgtc cgatggaaag atcatcgcgg accacgcaca ggaggtggat
     1741 tgggaatgaa tctgtggcag gctttcaaaa tggccttcaa gtccatctcc atgaaaaaga
     1801 cccg
//
LOCUS       DBMDSJ010000380         1802 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14528_length_1802_cov_1.864198, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000380 DBMDSJ010000000
VERSION     DBMDSJ010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14528_length_1802_cov_1.864198"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            217..>1802
                     /locus_tag="ACZPAQ_08385"
     CDS             217..>1802
                     /locus_tag="ACZPAQ_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR0904047.1"
                     /translation="MLRSLHMKLVMLMVLLILSLMTVVGAFLINSVVAFYIQDFYSQM
                     STVFGDQEIVSDLRTAADGETDGAGALKTVLNAYAGQLGVDTRNRNFYILDGEGTSVL
                     ASSNGESGASGLSYTGNLMTAIESGQVGDESNAAADYMDVAIPIQRGGSDYIIYILDN
                     KATVSNLNNQLFLLIMEALLFGLVISVLLSFLLSKTMITPIQRLTEGAMRVAEGDFAH
                     KIEVASRDEIGVLTDTFNDMAGQLQDTIRQVENERNKLNTLFLHMTDGVVAFSRTGEV
                     IHFNPSAEEMLHRPIGTDTTFGELFGGLVSLERVLAVPDYLQEEQTVEERFLQLLMAP
                     FDRDRQGGVLVVIHDVTEQRKNEEMRREFVANVSHELRTPLTNIRSYAETLVDSAGDL
                     PPAMEKKFLGVILNESDRMTHIVQDLLTLSRFDSGRTDLKLTQFSFSAVLHDLYNAVY
                     MEAQRHSHALELKIEPELPEIVADRERVVQVMMNIVSNSIKYTPDGGRIVISAGRRGD
                     RVWMLVDDNGIGIPPEDRPR"
BASE COUNT          352 a          553 c          585 g          312 t
ORIGIN      
        1 gagtcctgcg ggaaactgac cccgccagtc ctaaattcgt agtgacaaag cgggggatgg
       61 ggcactgctt cggcggctga gccggagacc gcccagggtg ccgcagaggc catttcccag
      121 ggcgccgagg tgtggtgcct gtgaccggca ggcggatgga acagagcccg cccccctgag
      181 acagagggac ggagagagaa gagagaaggt gccccgatgc tgcgcagcct gcacatgaag
      241 ctggtgatgc tgatggtgct gctcatcctg tcgctgatga cggtggtggg ggctttcctc
      301 atcaactctg tggtggcctt ttacatccag gacttttaca gccagatgag cacagtgttc
      361 ggagaccagg agatcgtctc cgacctgcgc accgctgcgg atggggagac cgacggggca
      421 ggggcgctga agacggtgct gaatgcctat gccggccagc tgggcgtgga tacccgcaac
      481 cgcaatttct acatcctgga cggggagggg acctcagtgc tggccagctc caacggtgag
      541 agcggcgcct ccggcttgtc ctacaccggg aacctgatga cggctatcga gtccggccag
      601 gtgggagacg agagcaacgc ggccgccgat tacatggatg tggccatccc catccagcgg
      661 ggcggcagcg actatatcat ctatatcctg gacaacaagg ccaccgtgtc caacctgaac
      721 aaccagctct tcctgctgat catggaggcg ctgctgttcg gtctggtgat ctcagtgctg
      781 ctgtccttcc tcctctccaa gaccatgatc actcccatcc agcggctgac ggagggagcc
      841 atgcgggtgg cagaggggga cttcgcccat aagatcgagg tggcctcccg ggacgagatc
      901 ggcgtgctca ccgacacctt caacgatatg gcgggtcagc tccaggacac catccgccag
      961 gtggagaacg agcgcaacaa gctcaacacc ctctttctcc acatgacgga cggcgtggtg
     1021 gccttctccc ggactggaga ggtcatccac ttcaatccct ccgcggagga gatgctccac
     1081 cggcccatcg ggacggacac caccttcggg gagctgttcg gcggcctggt ctctctggag
     1141 cgggtgctgg cggtgcccga ctacctccag gaggagcaga cggttgagga gcgattcctc
     1201 cagctgctga tggctccctt cgaccgggac cggcaggggg gcgtactggt ggtcatccac
     1261 gacgtcaccg agcagcggaa gaacgaggag atgcgccggg agttcgtggc caacgtgtcc
     1321 cacgagctgc gcacccccct caccaacatc cgcagctatg ccgaaacgct ggtggacagc
     1381 gccggagacc tgcccccagc catggagaag aagttcctgg gagtcatcct caacgagagc
     1441 gaccgcatga cccacatcgt gcaggacctg ctcaccctgt cccggttcga ctcggggcgc
     1501 accgatttga agctgaccca gttctccttt tctgcggtgc tccatgacct gtataacgcg
     1561 gtctatatgg aggcccagcg ccacagccat gcccttgagt tgaagatcga gccggagctg
     1621 cccgagatcg tggccgaccg ggagcgagtg gtccaagtga tgatgaacat cgtgtccaac
     1681 tccatcaagt acacgccgga cggagggcgc atcgtcatct ccgccggacg gcggggggac
     1741 cgggtgtgga tgttggtgga tgacaacggc atcggcatcc cgccggagga ccggccccgc
     1801 at
//
LOCUS       DBMDSJ010000381         1796 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14589_length_1796_cov_1.392920, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000381 DBMDSJ010000000
VERSION     DBMDSJ010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14589_length_1796_cov_1.392920"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="ACZPAQ_08390"
     CDS             complement(<1..530)
                     /locus_tag="ACZPAQ_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme domain-containing
                     protein"
                     /protein_id="HIR0904048.1"
                     /translation="MKKPFVTLEQLQAITEQYPTPFHLYDEKGIRENARRLYQAFSWN
                     PGYREFFAVKATPNPQILKILREEGCGVDCSSLTELMMSDRCGFRGEEIMFSSNDTPA
                     EEFALASQLGATINLDDITHIDFLKDTIGSIPKRISCRYNPGGTFTLGESEEGFQVMD
                     NPGDAKYGMTREQLTQA"
     gene            902..>1796
                     /locus_tag="ACZPAQ_08395"
     CDS             902..>1796
                     /locus_tag="ACZPAQ_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR0904049.1"
                     /translation="MNPNEPIRDARKLGTPKMLVLGLQHMFAMFGATILVPILVQQYG
                     LPLDIQTTLFFAGIGTLFFHVCTKFRVPAFLGSSFAFLGGFSTMANLNTGIYADMSPQ
                     DKLAYTCGGIVVAGLLYLALALVIRLVGVKKVMRFLPPVVTGPIIVCIGLTLAPSAVS
                     NASTCWPLALIALATIIIFNIWGKGMFKIIPILMGVVVSYIAALIMNACGVTVLNAAG
                     EAIPLIDFTAVREASILGLPPFQMAKFDLTAILVMAPIALATMMEHIGDMSAISATVG
                     QNFIQEPGLHRTLVGDGIATSL"
BASE COUNT          305 a          569 c          517 g          405 t
ORIGIN      
        1 gcctgggtca gctgctcccg ggtcatgccg tacttggcgt cccccgggtt gtccatgacc
       61 tggaagccct cctcagactc ccccagagtg aaggtcccgc ctgggttgta tcggcaggag
      121 atccgcttgg gaatggatcc aatggtgtcc tttagaaagt cgatgtgggt gatgtcgtcc
      181 aggttgatgg tggcccccag ttgggaggcc agggcaaact cctccgccgg ggtgtcgttg
      241 gatgagaaca tgatctcctc tccccggaag ccgcagcggt cggacatcat cagctcggtg
      301 agggaggagc agtccacgcc gcatccctcc tcccgcagga tcttcaggat ctgggggttt
      361 ggggtggcct tgacggcgaa gaactcccgg tagccggggt tccaggagaa agcctggtac
      421 aggcggcggg cattctcccg gattcccttt tcgtcgtaca ggtggaatgg ggtgggatac
      481 tgctctgtga tggcctggag ctgctccagg gtgacaaagg gctttttcac gggcagacgc
      541 tcctttttca aatctgtcag gcggtcccgg gccggatagc ccgccgcccc aagaaaaaaa
      601 tcgtttttat tgtatcgtat cccccatacg ttgtcaatca acacaacctc caacctgttc
      661 ccccacccgg gccctccttt ggtgattccg gaaaaaaaga cgaaattcgg caataagact
      721 tgcgtgtctc ccgctcttct ggtatgattg ttcggaaatg gaagggaggg gaggtccatg
      781 accgtgctgt acgggtcgct gtatcgggtt tgggggcctc tcttctgttc cgcaggcggg
      841 caatcaccgg tgtgattgtc tttttttgtg tgtcagagaa agtgagaagg gagttttatc
      901 catgaatcca aacgaaccca tccgcgacgc ccggaagctg ggcaccccca agatgctggt
      961 gctgggcctc cagcacatgt tcgccatgtt cggcgctacc atcctggtcc ccatcctggt
     1021 ccagcagtac ggtctgcccc tggacatcca gaccactctg ttctttgccg gcatcggcac
     1081 cctgttcttc catgtgtgca ccaaattcag ggtgcctgcc tttctgggct cctcctttgc
     1141 cttcctgggc ggcttcagca ccatggccaa tctgaacacc ggcatctatg cggacatgtc
     1201 tccccaggat aagctggcct atacctgcgg cggcatcgtg gttgccggcc tgctctacct
     1261 ggcgctggcc ctggtcatcc gcctggtggg cgtgaagaag gtcatgcgct tcctgccccc
     1321 ggtggtcacc ggccccatca ttgtgtgcat tggcctgacc ctggccccct ccgcggtgag
     1381 caacgcctcc acctgctggc ctttggccct gatcgccctg gccaccatca tcatcttcaa
     1441 catctggggg aaggggatgt tcaagatcat ccccattctg atgggcgtgg tggtctccta
     1501 tatcgccgcc ctcatcatga acgcctgcgg cgtcactgtg ctgaacgccg caggagaggc
     1561 catccccctc atcgatttca ccgccgtgcg cgaggccagc atcctgggcc tgcccccctt
     1621 ccagatggcc aagtttgacc tcaccgctat cctggtgatg gcccccatcg ccctggccac
     1681 catgatggag cacatcggcg acatgtccgc catctccgcc accgtggggc agaacttcat
     1741 tcaggagccc ggcctgcacc gcaccctggt gggcgacggc atcgccacct ccctgg
//
LOCUS       DBMDSJ010000382         1786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14680_length_1786_cov_1.706231, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000382 DBMDSJ010000000
VERSION     DBMDSJ010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14680_length_1786_cov_1.706231"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="ACZPAQ_08400"
     CDS             complement(<1..1155)
                     /locus_tag="ACZPAQ_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR0904050.1"
                     /translation="MIDIAISGLVKEFEVGKKILDGLTFQVDSGERVGLLGKNGCGKT
                     TLLRILTGQLDWDEGEVVLPPDKRVGLISQIPVYPAGYTVEDVLDTAFRPLREMEEEM
                     EQLAARMERGEDPALLRRYDQLTAAFEAGGGYDTDTRKNKVCSGLQIGPGMREQLFDR
                     LSGGEKTRVNLGRLILEDTDILLLDEPTNHLDLKATEWLEEYLDKFKGTVLAVSHDRW
                     FLDRVVDRVIEIQEGKAEFYSGNYSFYVVEKERRYQEKLKQYEKEQAKIQQLEKAAEQ
                     LRIWAYSGNDKIFKRAQSMEKRIERMRTTDRPTRERKMEVRFGEREFRGDEVLTIKGL
                     SKSFGQRALFSGVDLEVVGGERIALLGDNGTGKSTLIKILMGEEGPDEGKI"
     gene            complement(1249..>1786)
                     /locus_tag="ACZPAQ_08405"
     CDS             complement(1249..>1786)
                     /locus_tag="ACZPAQ_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR0904051.1"
                     /translation="HMIYPALIAAAIGERFGLTQDQIVEGILRFVPTRMRMNILYRGD
                     GIVILDDTYNANPQSMRAAISVLSDRRSQWSIAVLGDMFELGPFAPALHTGVGECLGK
                     ARIDCLVAVGELAEHIAQGARNSGVPLVYHCKDKAEAKPVLDQLVRPGSTILVKASRG
                     MELEELTAYLLSITPEAE"
BASE COUNT          259 a          638 c          548 g          341 t
ORIGIN      
        1 gatcttcccc tcgtcgggcc cctcctcccc catcaggatc ttgatgaggg tggatttgcc
       61 ggtgccgttg tcccccagca gggcgatgcg ctcgcccccc accacctcca ggtccacccc
      121 ggagaacagg gcgcgctggc cgaaggattt ggacagcccc ttgatggtga gcacctcatc
      181 cccccggaac tcccgctccc cgaagcggac ctccatcttc cgctcccggg tgggcctgtc
      241 ggtggtccgc atccgctcga tgcgcttctc catggactgg gcccgcttga agatcttgtc
      301 gttgcccgaa taggcccaga tccgcagctg ctcggcggcc ttctccagct gctggatctt
      361 ggcctgctcc ttctcatact gcttcagctt ctcctggtag cggcgctcct tctccaccac
      421 atagaagctg taattgccgg agtagaactc tgccttcccc tcctggatct cgatgacccg
      481 gtccaccacc cggtccagga accagcggtc gtgggagacc gccagcacgg tgcccttgaa
      541 cttgtccaga tactcctcca gccactcggt ggccttcagg tccaggtggt tggttggctc
      601 gtccagcagc aggatgtcgg tgtcctccag gatcagccgg cccaggttca cccgggtctt
      661 ctccccgccg gagagccggt cgaagagctg ttcccgcatc cccgggccga tctgaaggcc
      721 gctgcacacc ttgttcttcc gggtgtcggt gtcatagccg cccccggcct cgaaggcggc
      781 ggtgagctgg tcgtaccggc gcagcagggc ggggtcctcc ccccgctcca tccgggcggc
      841 cagctgctcc atctcctcct ccatctcccg cagggggcgg aaggcggtgt ccaatacatc
      901 ctccaccgtg tagccggcgg ggtagaccgg gatctgggag atcaggccca cccgcttgtc
      961 cgggggcagg accacctccc cctcgtccca gtccagctgg cccgtgagga tgcgcagcag
     1021 ggtggtcttg ccgcagccgt tcttgcccag cagtcccacc cgctcgccgg agtccacctg
     1081 aaaggtgagc ccgtccagta tttttttgcc gacctcaaat tccttgacca ggccggaaat
     1141 ggcgatatcg atcataacgg tcctctgtct ctcctgtgtt ggcctctggg aagggccctt
     1201 ccgcaggcgg gccttccaaa cggtccgtcc gcgggagggc cctgggggtc actccgcctc
     1261 cggggtgatg gagagcaggt aagcggtcag ctcctccagc tccatcccgc gggacgcctt
     1321 cactaaaatg gtgctgcccg gccggaccag ctggtccagg acaggcttgg cctccgcctt
     1381 gtctttgcag tggtacacca ggggcacgcc ggagttccgg gccccctggg cgatgtgctc
     1441 cgccagctcc cccaccgcca ccaggcagtc gatgcgggcc ttgcccaggc actcccccac
     1501 ccccgtgtgc agggcggggg cgaagggccc cagctcgaac atgtccccca gcacggcgat
     1561 gctccactgg ctgcgccggt cggacagcac gctgatggcc gcccgcatgg actgggggtt
     1621 ggcgttgtag gtgtcgtcca ggatcacgat cccatccccc cggtagagga tattcatccg
     1681 catccgggtg gggacgaagc ggaggatgcc ctccacgatc tggtcctggg tgagcccgaa
     1741 ccgctctccg atggcggcgg cgatgagggc ggggtagatc atgtgc
//
LOCUS       DBMDSJ010000383         1782 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14725_length_1782_cov_1.978584, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000383 DBMDSJ010000000
VERSION     DBMDSJ010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14725_length_1782_cov_1.978584"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="ACZPAQ_08410"
     CDS             complement(<1..1008)
                     /locus_tag="ACZPAQ_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019310.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0904052.1"
                     /translation="MVSLRRPHYAWIICLGGALSLFAVMGMGINVFSVYQPYIIQLNG
                     FSNAQGSWITTTRSLFTLAAMLTVNQLCARIGLRLVMTLGVVLLALSCFCFGLADSFP
                     LYCAAGALTGLAYGYGGMVPLSLAVGHWFRDRRGLALGLAAAGSGVSTIFVPTLITGI
                     IQAHGMRAAFLLEGLAVLVLGLLVFLLVRDVPGDLGLEPYHLGGPETPAPPPRPAPAG
                     MTPALRLSLLLAVFLIGGPGGPGFSHLTVLYTTAGYDSASAALLISYLGLVICVSKIL
                     CGQIYDRFGSRAGNWYVHLVFLAGLLLCCLAHLGGMLPAFAAMTLFGLGLPISAVKAG
                     SI"
     gene            1107..>1782
                     /locus_tag="ACZPAQ_08415"
     CDS             1107..>1782
                     /locus_tag="ACZPAQ_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR0904053.1"
                     /translation="MATLKYWLWLTGRRGLGNAGALRVLDHFVTPERAFYADPEEYAL
                     VEDLTPAARRALLDKDTGGAERILEDCARLDLRIMTLQDADYPERLRQLPDPPLALYI
                     RGRTFAFDEEAAVCMVGAREATPYGIGSAGRLGLELARGGALVVSGMAQGIDTASVQG
                     ALKGGGPVVSVLGGGVDVPYPRENRWLYEDVAAAGALISEYPPGTEHRGEHFPVRNRI
                     LSGLCLG"
BASE COUNT          332 a          558 c          673 g          219 t
ORIGIN      
        1 gatgctcccc gctttcaccg ccgagatggg caggcccagg ccgaacaggg tcatggccgc
       61 gaaagcgggg agcatcccac ccaggtgggc cagacagcac aggagcagtc cggccagaaa
      121 gaccaaatgg acgtaccagt tgcccgcccg gctgccaaag cggtcataga tctggccgca
      181 gaggatcttg gatacgcaga tgaccagccc caggtaagag atgagcagag cggcggaggc
      241 gctgtcatag ccggcggtgg tgtagagcac cgtgaggtgg gagaaaccgg gcccgccggg
      301 gccgccgatg agaaagacag ccagtagcag ggacagccgg agggcgggag tcatgcccgc
      361 cggtgcgggg cggggcggcg gcgccggagt ctccggaccg cccaggtggt agggctccag
      421 gcccaggtcg ccggggacgt cccggaccag gagaaagacc agcagcccca gcaccagcac
      481 cgccagcccc tccagcagaa aagcggcgcg catgccgtgg gcctggatga tcccggtgat
      541 cagcgtgggg acaaagatgg tggacacgcc gctgcccgcg gcggccagcc ccagagccag
      601 gccccgccgg tcccggaacc agtggcccac cgccagggag agggggacca tgccgccgta
      661 gccgtaggcc agaccggtga gggcccccgc ggcacagtag agggggaaac tgtcggccag
      721 gccgaagcag aagcaggaca gggccagcag cactaccccc agggtcatca ccagacgcag
      781 tccgatccgg gcgcacagct gattcactgt cagcatggcg gccagggtga agaggctgcg
      841 cgtggtggtg atccaggagc cctgggcgtt ggaaaagccg ttgagctgga tgatgtaggg
      901 ctgatacacg gagaacacgt tgatgcccat ccccatgacc gcgaacaggg acagggcgcc
      961 gcccagacag atgatccagg cgtaatgggg ccgccgaagg gagaccatgg gaacagcacc
     1021 tccttattcc tatctcctac catacaggag gcggggacgt ttgtaaaggg ggattttacc
     1081 ggaggagcgg gacggaggga agaaaattgg cgacgctgaa atactggctg tggctcaccg
     1141 gccgcagggg gctggggaat gccggcgccc tgcgggtgct ggaccacttt gtcaccccgg
     1201 agcgggcctt ctacgccgac ccggaggagt acgccctggt ggaggacctg accccggcgg
     1261 cccggcgcgc cctgctggac aaggacaccg gcggggcgga gcgcatcctg gaggactgcg
     1321 cccgcctgga cctgcggatc atgaccctcc aggacgccga ctacccggag cggctgcggc
     1381 agctgcccga cccgcctctg gcgctgtaca tccggggccg gaccttcgcc ttcgacgagg
     1441 aggcggcggt gtgcatggtg ggggcacggg aggccacccc ctatgggatc ggctcggcgg
     1501 ggcggctcgg cctggagctg gcccggggcg gggccctggt ggtgtctggc atggcccagg
     1561 gcatcgatac ggccagcgtc cagggcgctt tgaagggcgg cggaccggtg gtgtcggtgc
     1621 tggggggcgg agtggatgta ccctaccccc gggagaaccg ctggctctat gaggacgtag
     1681 ccgccgccgg ggccctcatc tccgagtacc cgccggggac ggagcaccgg ggggagcact
     1741 tcccggtgcg caaccgcatc ctcagcggcc tgtgcctggg gg
//
LOCUS       DBMDSJ010000384         1767 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14882_length_1767_cov_1.075630, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000384 DBMDSJ010000000
VERSION     DBMDSJ010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14882_length_1767_cov_1.075630"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="dnaN"
                     /locus_tag="ACZPAQ_08420"
     CDS             <1..998
                     /gene="dnaN"
                     /locus_tag="ACZPAQ_08420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR0904054.1"
                     /translation="GEQLRLTGYNLETGIRAAVPAEIREKGSLVLSARLFGEIIRKMP
                     DDVVVFTSENYMVNIKCGLSEFNILGTDPEEFPELPSVEEQNGLTIGQPVLRSMIAQT
                     LFAVSDNESRPVHTGSLFEVDESGLTVVSVDGYRLALRHEPVDSSQGVLPFSFVVPGS
                     ALSEVEKICAGEDAVSVAQGARHIQFQAGDTVLVCRRLEGEFLAYKNAIPRNNTIKIE
                     GDAHTLLQSIERVSLIISDKLKSPLRCVFGDGMLSITTKTGIGDAADQCPITGDGQGL
                     EIGFNNKYLMDALKAAPADRLRLEFTSGVAPCVILPAEGEENFVYMVLPVRLKAN"
     gene            1012..1227
                     /locus_tag="ACZPAQ_08425"
     CDS             1012..1227
                     /locus_tag="ACZPAQ_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR0904055.1"
                     /translation="MQSETIQIHTEFIKLQDLLKFAGAVETGGDAKLIIQEGRVAVNG
                     EVCTMRGKKLRPGDRAVIDDETELVVQ"
     gene            1224..>1767
                     /locus_tag="ACZPAQ_08430"
     CDS             1224..>1767
                     /locus_tag="ACZPAQ_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR0904056.1"
                     /translation="MIVKSLELDFFRNYVHLEAVFDPRVNLIYGDNAQGKTNLLEAVA
                     YLSTARSHRARYDREMIMLGVDGAFVKGEVRSRARDFTLEARLNRGARRQLWSNGVRL
                     KTAGELSGILNTVLFCPEDLYLIREGGAARRRFLDSAICQLRPRYEQALSEYNRLYDH
                     KVRILRDWQENLSLLSTLDDF"
BASE COUNT          332 a          533 c          589 g          313 t
ORIGIN      
        1 ccggagaaca gctgaggctc accggctaca acctggagac cggcatccgg gccgctgtcc
       61 cggctgagat ccgggagaag ggctcattgg tcctctccgc ccggctgttt ggcgagatca
      121 tccggaagat gccggatgac gtggtggtat tcacctcgga gaactatatg gtcaacatca
      181 agtgcggcct gtcggaattc aacatcctgg ggacggaccc ggaagaattt cccgaactgc
      241 cctcggtgga ggagcagaac gggctgacca tcggccagcc ggtgctgcgc tctatgatcg
      301 cccagaccct gttcgcggtg agcgacaacg agagccgccc cgtccacacc ggctccctgt
      361 tcgaggtgga cgagagcggc ctgaccgtcg tgtctgtgga cggctaccgg ctcgcccttc
      421 ggcacgagcc tgtggacagc agccaggggg tgctcccctt ctccttcgtg gtgcctggct
      481 ccgccctcag cgaggtggaa aagatctgcg ccggggagga tgctgtctcc gtggcccagg
      541 gggcccggca catccagttc caggcggggg acacggtgct ggtctgccgc cggctggagg
      601 gggagttcct ggcctataag aatgccatcc cccggaacaa caccatcaag atcgaggggg
      661 atgcccacac cctgctccag tccatcgagc gcgtctccct gatcatcagt gacaaactga
      721 agagccccct gcgctgtgtc ttcggggatg ggatgctgtc catcaccacc aagacgggca
      781 tcggggatgc cgccgaccag tgccccatca cgggggacgg ccaggggctg gagatcggct
      841 tcaacaacaa gtacctgatg gacgcactga aggccgcccc agcagaccgc ctgcgcctgg
      901 agttcacctc cggcgtggcc ccctgtgtca tcctgcccgc cgagggggag gagaatttcg
      961 tctatatggt gctgccggtg cggctgaagg caaactgaga ggaagatttt gatgcagagc
     1021 gagaccattc agatccacac cgaattcatc aagctccagg acctgctgaa attcgccggt
     1081 gcggtggaga ccgggggaga cgccaagctc atcatccagg agggacgggt ggcggtcaac
     1141 ggtgaggtgt gcaccatgcg ggggaagaag ctgcgtccgg gagaccgggc ggtcattgac
     1201 gatgagacgg aactggttgt ccaatgatcg tcaagtcgct ggagctggac tttttccgta
     1261 actatgtcca tctggaggcc gtctttgatc cacgggtgaa cctgatctat ggagacaacg
     1321 cccagggcaa gaccaacctg ctggaagcgg tggcctatct gtccaccgct cggtcccacc
     1381 gggcccggta cgaccgggag atgatcatgc tgggagtgga tggggccttt gtcaagggag
     1441 aggtgcgctc ccgggcccgg gacttcactc tggaggcccg gctgaaccgg ggggcccggc
     1501 gccagctgtg gtccaacggg gtgcggctga agacggcggg ggagctgtcc ggcattttga
     1561 ataccgtgct tttctgcccg gaggacctgt atctcatccg ggagggaggg gcggcccggc
     1621 gccgctttct ggacagcgcc atctgccagc tgcggccccg gtatgagcag gcactcagcg
     1681 agtataaccg cctctatgac cacaaggtgc gcatactccg ggactggcag gagaacctgt
     1741 ccctgctgtc caccctggac gacttca
//
LOCUS       DBMDSJ010000385         1765 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14893_length_1765_cov_1.787260, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000385 DBMDSJ010000000
VERSION     DBMDSJ010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14893_length_1765_cov_1.787260"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="ACZPAQ_08435"
     CDS             <1..1068
                     /locus_tag="ACZPAQ_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024816.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0904057.1"
                     /translation="LEEQTQQFLRELSALRREGADPAVLGRLEVATALIQGRIALFQG
                     DSARGSDILGALSGGSIHPDTGVLIRACYLLASSALYRQATEQVERYTATGMRLLERT
                     RDPVQQAQFQRLRGGLFCLQGAYDKSRYYLLEAMDALRGQPRSTAVRLQLAAVYCDYG
                     RVCRQRLEYADACSYYKRALSLLGDLPWPGGVWVYVHYGRAAFALEDHPRARELFQHG
                     YDNAKVSGELWGRTAASAYTAYYQAMEGDYERAAVSLADAQSCQQRLNSPLEGVILHF
                     VSMHIRSRLDLEQRTGSPLAPLLTYSPESYARQGVRLLSSVPSVFEAEQLSQSLRNGI
                     ATQQRYRASELYSKNKHFMAE"
     gene            1372..>1765
                     /locus_tag="ACZPAQ_08440"
     CDS             1372..>1765
                     /locus_tag="ACZPAQ_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HIR0904058.1"
                     /translation="MSFETVENVLNINTDAIMTLAMASILLLIGYAIKNRVNFLAKYC
                     IPAPVIGGFIFMFITFAGHTTGTFTFNFTNTFQDPFMLAFFTTVGLGASFSLLKKGGI
                     LLIVYWLCAGVVSIFQNIIGIAVGTAVGL"
BASE COUNT          312 a          572 c          514 g          367 t
ORIGIN      
        1 ctggaggagc agactcagca gttcctgcgg gagctgtccg ccctgcgccg tgagggggcg
       61 gaccccgcag tcctgggccg tctggaggtg gccaccgctc tgatccaggg gcgcatcgcc
      121 ctgttccagg gggacagcgc ccggggcagc gatatcctgg gcgccctcag cggcggcagc
      181 atccaccccg acacgggggt gctcatccgg gcctgctacc tgctggcctc ctccgccctc
      241 taccgtcagg ccactgaaca ggtggagcgc tacaccgcca ccggcatgcg cctgctggag
      301 cgcacccggg acccggtcca gcaggcccag ttccagcggc ttcggggcgg cctgttctgt
      361 ctccagggcg cctatgacaa gagccgctac tatctgctgg aggccatgga cgctctgcgg
      421 ggacagcccc gctccaccgc ggtccggctc cagctggcgg ccgtctactg tgactacggc
      481 cgggtctgcc gccagcggct ggagtacgcc gacgcctgct cctactataa gcgggccctc
      541 tccctgctgg gagacctgcc ctggcccggc ggcgtctggg tctatgtcca ttacgggcgg
      601 gccgcttttg ccttggagga ccatcccagg gcccgtgagc tgtttcagca tgggtatgac
      661 aacgcgaaag tttccggcga gctgtggggc cggacagccg cctcagccta caccgcctat
      721 tatcaggcca tggaggggga ctacgagcgg gcggcggtca gcttggccga tgcccagtcc
      781 tgccagcagc ggctgaattc ccctctggag ggggtcatcc tccactttgt ctccatgcac
      841 atccgcagcc ggctggacct ggagcagcgg acgggctccc ccctcgcccc tctgctcacc
      901 tactccccgg agagctacgc ccggcagggg gtccgcctgc tttccagcgt ccccagcgta
      961 ttcgaggcgg agcagctctc ccagagcctg cgcaacggca tcgccaccca gcagcggtac
     1021 cgtgcctctg agctgtacag caaaaacaag cattttatgg cggaatgagc agtaaccgcc
     1081 cccacatgat cgccattttt atagatgcgc ctaaaattta tcggtgcgtc ttgacagatg
     1141 ctcttctatg gatatattat ggacacgtaa agacgcagcg gtccgtttgg actgcggcgt
     1201 cttttttcaa tttttagaat tagacgtttt ttagatgctc tttgactata ctggcgtcag
     1261 aaacgtctaa ttctgttccg gcgaaaggag gtcggctggc cggggcagtg tttcggtccg
     1321 taaggagagc ggtccgagga aaaaagagaa tcaaaatggg agggtaacat catgtcattt
     1381 gaaaccgtgg aaaacgtcct caacatcaac accgatgcaa tcatgaccct ggcaatggct
     1441 tccattcttc tgctgatcgg ttacgcgatc aaaaacaggg tcaacttcct ggccaagtac
     1501 tgtatccctg ctcccgtcat tggcggcttc atctttatgt tcatcacctt cgccggccac
     1561 accaccggta cttttacctt caacttcacc aacaccttcc aggacccctt catgctggcc
     1621 ttcttcacca ccgtgggtct gggcgccagc ttctccctgc tgaagaaggg cggcatcctg
     1681 ctgatcgtct actggctgtg cgccggtgtg gtctccatct tccagaacat catcggcatc
     1741 gccgtgggca ccgcggtagg tctgg
//
LOCUS       DBMDSJ010000386         1759 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_14952_length_1759_cov_1.651990, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000386 DBMDSJ010000000
VERSION     DBMDSJ010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14952_length_1759_cov_1.651990"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(439..>1759)
                     /gene="ilvD"
                     /locus_tag="ACZPAQ_08445"
     CDS             complement(439..>1759)
                     /gene="ilvD"
                     /locus_tag="ACZPAQ_08445"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR0904059.1"
                     /translation="PGCDKIVPGMLMVAMRLNIPAIVIPGGPMLPGKYKDMEAITLTD
                     MRELIGRTQTGRLTEDELMKIEEAALPGVGTCSMLGTANTMSCLAEALGMSLPGCGLA
                     HAVSAKKRRIAKQSGRRIVAMVKEDLTPRKIMTREALRNAIRVSMAMGASTNSTLHLP
                     AIAHEAGIHITLDDFDELSREIPYLCNIKPSGQYPLSVLEEQGGVPAVMKALERKLDL
                     EQMTVSGKTVGENLAGVELVENDVIFSLDHPKKPEGGLAILHGNLAPKGAVVKQAGVK
                     PSMHYFEGQARVFTSMEEASWAVSNDKIQKGDVLVIRYEGPKGGPGMREMHMTCSLIV
                     GRGMDEDCALVTDGRFSGSTRGPCIGHVSPEAAAGGPIAAVEEGDRIIIDIPNRTLTL
                     CVPEEEIQRRLERVRHIHKPATPALERYAALVTSADQGAVLQLPRER"
BASE COUNT          296 a          568 c          542 g          353 t
ORIGIN      
        1 tgtgcagaag gaagccgatt cctttcactt tggaacaaaa ttttttgtcc cggctgccga
       61 agctgcgggc aaagcgggac ggaagcagca gaagacctcc ctgcggaaca gcagctccag
      121 cagggaggtc ttgtctcgac tctcgtggtc attcttttgt atgcgggcct gcgcagggga
      181 gagggccgcc gtctcgttat ctcagggtgg gattgccccc ctgcccggtg ggaatgtcca
      241 gcaggatgtc cttgttcttt cccgcccgca gcacatagct gtctcccggg tggcccatgg
      301 gcccctcagc gcgcagtccc gtagccctac ctcgcaaacg gtgatgcgct caggcagctt
      361 cataccatat cacactccaa atcatatcag gcagaatatg ttcaaacacg gatgcttaca
      421 ccgaaacagc tgtagccgtc accgctcccg gggcagctgg agcaccgcgc cctgatcggc
      481 actggtgacc agggccgcgt agcgctccag cgccggggta gccggcttgt ggatgtgccg
      541 gacccgctcc aggcggcgct ggatctcctc ctccgggaca cagagggtca gggtgcggtt
      601 ggggatatcg atgatgattc tgtcaccctc ttccactgcg gcgatggggc cgccggcggc
      661 agcctccggg gacacgtggc cgatacaggg cccccgggtg gagccggaga agcgcccgtc
      721 ggtgaccagg gcacagtcct cgtccatccc acggcctaca ataagggagc atgtcatgtg
      781 catctcccgc atcccaggac cacccttggg cccctcgtac cggatgacca gcacgtcccc
      841 cttttggatc ttgtcgttgg acaccgccca gctggcctcc tccatggagg tgaagacccg
      901 ggcctgcccc tcaaagtagt gcatgctggg cttgaccccc gcctgtttca ccactgcgcc
      961 cttgggggcc agattgccgt gaagaatagc caggccgccc tctggcttct tggggtggtc
     1021 cagggagaag atgacatcgt tttccaccag ctcaaccccg gcgagatttt cgcccaccgt
     1081 ctttccactg accgtcatct gctccaggtc cagcttacgc tccagggcct tcatcaccgc
     1141 agggaccccg ccctgctcct ccagtacact gagggggtac tgcccggagg gcttgatatt
     1201 gcagagatag gggatctccc ggctcagctc atcaaagtca tccagggtga tgtggatgcc
     1261 cgcctcatgg gcgatggcgg gcagatgtaa ggtgctgttg gtggatgctc ccatggccat
     1321 gcttactctg atggcgttgc gcagggcctc ccgggtcatg atcttacggg gcgtcaggtc
     1381 ctccttcacc atggccacga tccgacggcc cgactgcttg gcgatgcgcc gttttttcgc
     1441 gctcaccgcg tgggccaggc cgcagccggg caggctcatg cccagggcct cggccagaca
     1501 gctcatggta ttggctgtgc ccagcatgga gcaggtgcca acgccgggca gggcggcctc
     1561 ctcgatcttc atcaactcgt cttcggtgag cctgccggtc tgggtgcggc caatcagctc
     1621 ccgcatgtcg gtgagagtga tcgcctccat gtccttgtac tttccaggga gcatgggccc
     1681 gcccgggatg acgatggcag ggatgttcag cctcatggcg accatcagca tgccggggac
     1741 gatcttgtcg cagccgggg
//
LOCUS       DBMDSJ010000387         1751 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15030_length_1751_cov_1.753333, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000387 DBMDSJ010000000
VERSION     DBMDSJ010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_1751_cov_1.753333"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="ACZPAQ_08450"
     CDS             <1..263
                     /locus_tag="ACZPAQ_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR0904060.1"
                     /translation="ALNMPIQGTAADIIKLAMLRVRDRLSTEGMEGRLVLQVHDELIV
                     ECPEEEQGRVCALVEEEMERVISLSVPLLAETSAGKSWADAH"
     gene            complement(336..>1751)
                     /locus_tag="ACZPAQ_08455"
     CDS             complement(336..>1751)
                     /locus_tag="ACZPAQ_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR0904061.1"
                     /translation="CFLGLARARPKKHRWLPLAAAACLVLAVTGGGVGYYLQSNTVAS
                     IISLDVNPGVELTVNRQEKVLSAAPTNEDAVDILDGMELKGTPLDVAMNAIVGSLVKN
                     GYVDELANSILITVEDEDTARGARLQQELTSQADAVLASAQVNGAILSQTFQHNDALQ
                     QKADEYGISAGKAALIQTIADSSSGLYTFEELVGLPINDLNLLYTSLFSFRPADPEDI
                     GVIGGSDGSSSVQTLPSSGMIQTSGQASDSAYIGVEAAQAAAFSHAGVSAAQATVLEI
                     DFDYEDGRMVYELEFYANGSEYEYDIDALTGAVVKSQQESRGSAPSGGAPSGADIGEQ
                     GAKEAALAHAGLTEGDVVSLSVKREYDDGRLEYEVKFWVDTTEYEYTVDGATGDIRKS
                     EREPHPSASSADIGQDQAVSIALAHAGLSQSQVYGLEAEREWEDDHLEYQVEFKAGGM
                     EYSYDIAASDGAILSFEREKD"
BASE COUNT          309 a          533 c          592 g          317 t
ORIGIN      
        1 tggccctgaa catgcctatt cagggaacgg cggccgacat catcaagctg gccatgctcc
       61 gggtgcggga ccggctgagt acggagggga tggagggccg gctggtcctc caggtccacg
      121 acgagctgat cgtggaatgc ccggaggagg aacaggggcg ggtgtgcgcc ctggtggagg
      181 aggagatgga gcgggtgatc tccctgtctg tccctctgct ggcggagact tcggcgggca
      241 agagctgggc ggatgcccac tgaggggaaa acagaaaaaa caggggagcc cggacggacc
      301 gttcaagtcc gtccgggctc cttgttttga aaagctcagt ccttctcacg ctcaaaggag
      361 aggatggcac cgtcagaggc ggcaatgtcg tagctgtact ccatgccgcc ggccttgaac
      421 tccacctggt actccagatg gtcgtcctcc cactcccgct ctgcctccag cccatagacc
      481 tgggactggg agagcccagc atgggccaga gcgatggata cggcctggtc ctggccgatg
      541 tcggcggagg aggcggaggg atgtggctcc ctctcgctct tgcggatgtc cccggtggcg
      601 ccatctaccg tgtactcata ctcggtggta tccacccaga acttgacctc atactccaga
      661 cggccgtcgt cgtactcccg cttgaccgac agggagacca catccccctc cgtcaacccg
      721 gcgtgggcca gggcggcctc cttcgctccc tgctcaccga tgtcggcgcc ggagggagcg
      781 ccgccggagg gggcggagcc ccggctctcc tgctgggatt tcaccacagc cccggtgagg
      841 gcgtcgatgt cgtactcata ctcgctgcca ttggcataga actccagctc atagaccatc
      901 cttccatcct catagtcgaa gtctatctcc agtacagtgg cctgggcggc ggagacgccg
      961 gcgtgagaga aggcggcagc ctgggcggct tccacgccga tgtaggcgct gtcgctggcc
     1021 tggccggagg tctggatcat gccgctgctg ggtagtgtct gaacggaaga ggagccgtcg
     1081 ctgccgccga tgacgccaat gtcctcgggg tcggcggggc ggaaggagaa gagggaggta
     1141 tagagcaggt tcaggtcgtt gatgggcaga cccaccagct cctcaaaggt gtaaaggccg
     1201 gaactgctgt ctgcgatggt ctggatcagg gccgccttgc cggcggagat gccgtactcg
     1261 tccgccttct gctggagggc atcattgtgc tggaaggtct gggagaggat ggcaccgttc
     1321 acctgggcac tggccaggac ggcgtccgcc tggctggtca gctcctgctg gagccgggcg
     1381 ccccgggcgg tgtcctcatc ttccaccgta atgaggatgg agttggccag ctcgtccaca
     1441 tagccgtttt tgaccaggga gcccacgatg gcgttcatgg ccacatccaa gggggtcccc
     1501 ttcagctcca tgccatccag gatatccacc gcatcctcgt tggtgggggc ggcggacagc
     1561 accttttcct gccggttcac cgtcagctcc acaccggggt tcacgtccag ggagatgatg
     1621 gaggctacag tgttggactg gaggtagtaa ccgacgccac ctcccgtcac agccagcaca
     1681 aggcaggcgg cagcggccag agggagccag cggtgcttct tgggtctggc ccgggccaga
     1741 cccaagaagc a
//
LOCUS       DBMDSJ010000388         1751 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15038_length_1751_cov_1.080606, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000388 DBMDSJ010000000
VERSION     DBMDSJ010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_1751_cov_1.080606"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(144..791)
                     /locus_tag="ACZPAQ_08460"
     CDS             complement(144..791)
                     /locus_tag="ACZPAQ_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904062.1"
                     /translation="MKLTAAGAALLAKVPAGSGAPVTRWQIGTGVLPAGTDLTARTAL
                     AEPLKDLPIARVTNSGSQSLVLGQFVNTGMDAFVWEELGLLAQDPDEGEILMGYGCAF
                     GAGEAIQAGEDQLREFVFGCQLIFSGETAVTAEIDRTLVFATLKDLENYYTKGETLSP
                     ETRVLLGDVNTPDEAFLALYGMAGSIVEMPPAVEPEDRRDGTYYLDRKVSYNRGV"
     gene            complement(793..1314)
                     /locus_tag="ACZPAQ_08465"
     CDS             complement(793..1314)
                     /locus_tag="ACZPAQ_08465"
                     /inference="COORDINATES: protein motif:HMM:NF021583.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HIR0904063.1"
                     /translation="MLNNLPVQYRRDPWLIVLAAALQVPMDRQGEVIRSLPSQISLDR
                     VTWNLPVEERVAGIVPPAGATVEQRRAALRAKWRSSGKATLGQIQAVAESWESQRVET
                     SFLAGVIGIDIINLRQGTVAEIREAVEKIKPAHLPLEITERQYVETPILVGALARQGD
                     SMILWEVDCRNDP"
     gene            complement(1304..>1751)
                     /locus_tag="ACZPAQ_08470"
     CDS             complement(1304..>1751)
                     /locus_tag="ACZPAQ_08470"
                     /inference="COORDINATES: protein motif:HMM:NF016736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR0904064.1"
                     /translation="VLVDQFGEPASEELVEQVQTHIDPDSKGIGEGEAPIGAYCYVSA
                     AVEQAVALSLTLVLAEGAEQETVEAAIRAAVSGYLKEQALEAYQPSLSPEKGYRVSYA
                     RIGSAILDTDGVEDYEGLTVNGGTANLTVPAKYAAVLGEVSVSYAE"
BASE COUNT          275 a          678 c          481 g          317 t
ORIGIN      
        1 agtattcata attcggatat ataccttcgg gtatgtttct atatccccgc cgaggctgac
       61 ataaagcgta gacccttggg tataacttac cctatatgac tgattaagag aatacttact
      121 ccaccgataa tacgtcgcca cacctacacc cccctgttat agctgacctt ccggtccagg
      181 tagtatgtcc cgtccctccg gtcctccggc tccaccgccg gcggcatctc cacgatggac
      241 cccgccatcc cgtacagcgc caggaacgcc tcgtcagggg tgtttacatc ccctaacagc
      301 acacgggtct ccgggctcag ggtctccccc ttcgtgtagt agttctccag gtccttcagg
      361 gtggcgaaca ccagcgtccg gtcgatctcc gccgtcacgg cggtctctcc ggagaagatc
      421 agctgacagc cgaagacgaa ctcccgcagc tggtcctccc ccgcctggat ggcctccccg
      481 gccccaaagg cgcagccgta gcccatcagg atctccccct cgtccgggtc ctgagccagc
      541 aggcccagct cctcccagac aaaggcgtcc atcccggtgt tgacgaactg ccccagcacc
      601 aggctctggc tgcccgagtt ggtcacccgg gcgatgggca gatccttcag cggctccgcc
      661 agcgcggtgc gcgccgtcag gtccgtcccc gccggcagca cccccgtccc gatctgccac
      721 cgggtcaccg gcgccccgct ccccgccggc accttggcca gcagcgccgc ccctgcggcg
      781 gtcaatttca cactagggat cattcctgca atccacctcc cacaagatca tgctgtcccc
      841 ctgccgggcc agcgccccca caaggatggg ggtctccaca tactgtctct ccgtgatctc
      901 cagcggcagg tgggcgggct tgatcttctc caccgcctcc cggatctccg ccactgtccc
      961 ctgccggaga ttgatgatgt cgatcccgat cacgccggcc aggaaggagg tctccacccg
     1021 ctggctctcc cagctctccg ccaccgcctg gatctggccc agcgtcgcct ttccgctgct
     1081 gcgccacttt gcccgcagcg ccgcccggcg ctgctccacg gtggccccgg ccggcgggac
     1141 gatccccgcc acccgctctt ccaccggcag gttccaggtc acccggtcca gggagatctg
     1201 gctgggcagg gagcggatga cctccccctg ccggtccatc ggcacctgaa gggcggccgc
     1261 cagtacgatg agccaggggt cccgccggta ctggacgggc agattattca gcataggaga
     1321 cagacacctc ccccagcacc gcggcgtatt tggccgggac ggtcagattc gccgtcccgc
     1381 cgttgacggt cagcccctcg tagtcctcca ccccgtccgt gtccaggatg gcggagccga
     1441 tgcgggcata gctcacccgg tatcccttct ccggggagag ggacggctgg tacgcctcca
     1501 gcgcctgctc tttcaggtac ccggacaccg ccgcccggat cgcggcctcc acggtctcct
     1561 gttccgctcc ctccgccagc accagggtca gggacagcgc caccgcctgc tccaccgccg
     1621 cggacacgta gcagtaagcg ccgatgggag cctccccctc tccgatgccc ttgctgtccg
     1681 ggtcgatgtg ggtctgtacc tgctctacca gctcctcgct ggcgggttcc ccgaactgat
     1741 ccaccagcac c
//
LOCUS       DBMDSJ010000389         1743 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15104_length_1743_cov_1.510353, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000389 DBMDSJ010000000
VERSION     DBMDSJ010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15104_length_1743_cov_1.510353"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="ACZPAQ_08475"
     CDS             <1..494
                     /locus_tag="ACZPAQ_08475"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HIR0904065.1"
                     /translation="DGFAIGGLAVGEPTQTMYDIIDAVEPHMPREKPRYLMGVGTPSN
                     IIEGVARGIDFFDCVMPARNARHGKLFTWEGTINIKNEKYKLDDRPIDPACTCPTCAA
                     FSRAYVRHLLAAGEMLAMRLAVMHNLHFYNELMARIRQALDEGRFEAFRAEYSEKLGR
                     PAP"
     gene            533..1729
                     /gene="dpaL"
                     /locus_tag="ACZPAQ_08480"
     CDS             533..1729
                     /gene="dpaL"
                     /locus_tag="ACZPAQ_08480"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HIR0904066.1"
                     /translation="MEHFASVLRPRGQRPGADLTLTSPGEAEKARRFHSGLPGYAPTP
                     LVSLDGLAHSLGVSKIWIKDESKRFGLNAFKALGGSYAMARCLSEKLGKPLEEMSFEE
                     LCAPAARRALGDITFVTATDGNHGRGVAWAARLLGQKAVVYMPKGSAAERLENIRAQG
                     AQAEITEYNYDDAVRLANRMAEEHGWILIQDTAWPGYEEIPAHIMQGYTTLAAETLQQ
                     LEEQGAERPTHLFLQAGVGSFAGAALGFFTSVWGEERPVTAIVEPDRADCLFRTAQAD
                     DGALHFVTGDMDSMMAGLCCGEPCTISWKIIDAYADAFLSCGDGYAARGMRLLARPEE
                     GDPAVVSGESGAVTAGVLDALMRGDAPSEVRQALGLNESSRVLLVSTEGDTDQENYQR
                     IINGED"
BASE COUNT          304 a          550 c          616 g          273 t
ORIGIN      
        1 gtgacggctt cgccatcggg gggctggcgg tgggggagcc cacacagacc atgtacgaca
       61 tcatcgacgc ggtggagccc catatgcccc gggagaagcc ccgctacctc atgggggtgg
      121 ggaccccctc caacatcatc gagggcgtgg cccgtggcat cgacttcttc gactgcgtca
      181 tgcccgcccg gaacgcccgc cacggcaagc tcttcacctg ggagggcacc atcaacatca
      241 agaatgagaa gtataagctg gatgaccgcc ccatcgaccc cgcctgcacc tgccccacct
      301 gtgccgcctt ctcccgggcc tatgtccgcc atctgctggc ggcgggggag atgctggcca
      361 tgcggctggc tgtcatgcac aacctgcatt tctataacga gctgatggcc cgcatccggc
      421 aggccctgga cgaggggcgc tttgaggcat tccgggcgga atacagcgaa aagctgggga
      481 gaccggcgcc ctgaggatac gccgggaatg agatcgaaca ggagtgaatc gcatggaaca
      541 ctttgcatct gtcctgcgcc cgcgggggca gcgtcccggg gccgacctga ccctgaccag
      601 tcccggggag gcggagaagg cccgccgctt ccacagcggc ctgccgggct atgcccctac
      661 tccgctggta tccctggacg gcctggccca cagtctgggc gtgtccaaga tctggatcaa
      721 ggatgagtcc aagcggttcg gcctcaacgc cttcaaggcg ctgggcggga gctatgccat
      781 ggcccggtgt ctgtcggaaa agctggggaa gcccttggag gagatgagct ttgaggagct
      841 gtgcgccccc gccgcccgcc gagcgctggg ggacatcacc ttcgtcactg ccaccgacgg
      901 aaaccacggc cgcggagtgg cctgggcggc ccggctgctg ggccagaagg cggtggtcta
      961 catgcccaag gggagcgcgg cggagcggct ggagaacatc cgcgcccagg gcgcccaggc
     1021 ggagatcacc gagtataact atgatgacgc ggtccgcctg gcgaaccgga tggcggagga
     1081 gcacggctgg atcttgatcc aggacacagc ctggccgggc tatgaggaga tccccgctca
     1141 tatcatgcag ggctatacca ctttggccgc cgagaccctc cagcagctgg aggagcaggg
     1201 ggcggagagg cccacccatc tgtttctcca ggcgggggtg ggcagcttcg ccggtgcggc
     1261 gctgggcttc tttacctccg tatgggggga ggagcggccg gtcaccgcca tcgtggagcc
     1321 agaccgggcc gactgcctgt tccgcaccgc ccaggccgac gacggggcgc tgcacttcgt
     1381 cactggggac atggacagca tgatggcggg tctgtgttgc ggagagccct gcaccatcag
     1441 ctggaagatc atcgatgcct acgcggatgc cttcctgtcc tgcggggacg gatacgccgc
     1501 ccggggaatg cgtctgctgg cccggccgga ggagggcgac ccggcggtgg tgtccgggga
     1561 gtccggcgca gtgacagccg gggtgctgga tgccctgatg cggggagacg ccccgtcaga
     1621 ggtgcggcag gcgctgggtc tgaacgagtc ctcccgggtg ctcctggtga gcaccgaggg
     1681 ggacaccgac caggaaaatt accagcggat catcaacggg gaggactgat ccccatgttg
     1741 gtc
//
LOCUS       DBMDSJ010000390         1742 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15112_length_1742_cov_1.779403, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000390 DBMDSJ010000000
VERSION     DBMDSJ010000390.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15112_length_1742_cov_1.779403"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(54..266)
                     /locus_tag="ACZPAQ_08485"
     CDS             complement(54..266)
                     /locus_tag="ACZPAQ_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904067.1"
                     /translation="MDLRNNTITVGELLDNRASRSVFQRRFGKFMNHPLVGASRSLTL
                     AQLAEMAAVWLPQRTIQETIQELRQL"
     gene            complement(591..1376)
                     /locus_tag="ACZPAQ_08490"
     CDS             complement(591..1376)
                     /locus_tag="ACZPAQ_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR0904068.1"
                     /translation="MPILEVHHIEKHFGATRVLEDISFDLEQGQALAIIGSSGSGKTT
                     LLRCLNFLETPDQGQIVVQGDTLFDADDPATQRESEVRKKRLHFGLVFQSFNLFPQYT
                     ALRNVTLAKELLSQERPDFKANRKQILEEIQAEGRELLAKMGLSDRADHYPSQLSGGQ
                     QQRVAIARALALHPDILCFDEPTSALDPELTGEVLKVIRSLAEQKTTMIIVTHEMAFA
                     RDVADQVIFMDGGVIVEQGPAHQVIEHPSQERTRQFLSRYAES"
     gene            complement(1389..>1742)
                     /locus_tag="ACZPAQ_08495"
     CDS             complement(1389..>1742)
                     /locus_tag="ACZPAQ_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR0904069.1"
                     /translation="IYRGGIQGVPAGQNEAGLVLGMTKRQIFFKVTLLQMVKRIVPPM
                     SNEIITLVKDTSLARAIALQEVIWAAESFMKGNQGISGLWWPLMFTAVYYLAFSAIVT
                     MVLGRLEKKLDYFRV"
BASE COUNT          339 a          513 c          541 g          349 t
ORIGIN      
        1 cagacgcaca gggggcgtgc ctgctgggca cgccccctgt gcgttatgac atgttacagc
       61 tgacgcagct cctgaatggt ctcctggatg gtcctctggg gcagccagac ggcagccatc
      121 tccgccagct gagccagggt cagggaccgg gaggccccca ccagcgggtg gttcatgaat
      181 ttcccaaacc ggcgctggaa cacagaccgg gaggcccggt tatccagcag ctcccccacc
      241 gtaatggtgt tgtttctcag atccaaggtc atttcacctc cgctccattc tatgcagggg
      301 aggggaaaag aatgtggagt caggacggtg agactgttga aaaagtagct ctgccgcttt
      361 ttcgagcagg agcttagaaa agtttaccca caattctttg cagaattgtg ggtaaagatt
      421 tcatgaaaag catcatttct aaggcacatg ccctgagaaa tgacgcaatc aaaccttact
      481 cggctgcctg cgggcgctgg aactccgagc aggagttttt ttccacaagc tgagtgggag
      541 cttccttata caagaagctc ccactggtga ggtttaaaat tgtggtgtgc tcaactctcc
      601 gcataccggg agaggaactg ccgggtgcgc tcctgagagg ggtgctcgat gacctgatgg
      661 gctgggccct gctccacaat gacgccgcca tccatgaaga tgacctgatc ggccacatcc
      721 cgggcgaagg ccatctcgtg ggtgacgatg atcatggtgg tcttctgctc cgccagggag
      781 cggatgacct tgagcacctc accggtgagc tccgggtcca gggcggaggt gggctcatca
      841 aagcaaagga tgtccgggtg gagggccagg gcccgggcga tggccacccg ctgctgctgg
      901 ccgccggaga gctgactggg gtagtggtcc gcgcggtcac tcagacccat cttggccagc
      961 agctcccggc cctcggcctg gatctcctcc aggatctgct tccggttggc tttgaagtcg
     1021 ggccgttcct ggctgaggag ctccttggcc agggtgacgt tccgcagggc ggtgtactgg
     1081 gggaacaggt tgaaggactg gaacaccagg ccgaaatgga gccgcttttt ccgcacctcg
     1141 ctctcccgct gggtggcggg atcgtcggcg tcgaacagag tgtccccctg gacgacgatc
     1201 tgcccctggt cgggggtctc cagaaagttc aggcaccgca gcagggtggt cttgccgctg
     1261 ccggaggagc cgatgatggc cagagcctgg ccctgctcca ggtcaaagct gatgtcctcc
     1321 agcacccggg tcgcgccaaa gtgcttttca atatgatgta cttctaagat cggcatgttc
     1381 gctccccctt atacccggaa gtagtccagc ttcttttcca gccggcccag caccatggtc
     1441 acgatggcgc tgaaggccag gtagtagacc gccgtgaaca tcagcggcca ccacaggccg
     1501 gagatcccct gattgccctt catgaaggac tcagccgccc agatgacctc ctgcagcgca
     1561 atggcacggg ccagggaggt gtccttgacc aaggtgatga tctcgttgga catggggggc
     1621 acgatgcgct tgaccatctg gagcagggtg accttgaaaa aaatctgccg cttggtcatg
     1681 cccagcacca ggccggcctc gttctgcccc gccggcaccc cctggatgcc gccccggtag
     1741 at
//
LOCUS       DBMDSJ010000391         1735 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15177_length_1735_cov_2.050184, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000391 DBMDSJ010000000
VERSION     DBMDSJ010000391.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15177_length_1735_cov_2.050184"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..1525)
                     /locus_tag="ACZPAQ_08500"
                     /pseudo
     CDS             complement(1..1525)
                     /locus_tag="ACZPAQ_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981018.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
BASE COUNT          284 a          564 c          556 g          331 t
ORIGIN      
        1 ccagccgggc ggccgcccgg tcgatgatct cctccggcag gcccaggcgc cgggagatag
       61 cgaaggcgtt ggatttgccc gggatgccca ttaccagccg ttaggtgggg gccagggtgt
      121 ccacattgaa ctcgcaggag gcgttctcca ccccgggggt ggtcatagcg tacaccttca
      181 gctcggcata gtgggtggtg gccgccacca gtgcccccag ctcccgggca ctctcaatga
      241 cggccgccgc cagggcggcg ccctccaccg ggtcggtgcc tgcccccagc tcgtcgaaca
      301 ggatcagggt ctgaccgtcc gcctccttca ggatgcccac gatgttggtc atatgggagg
      361 agaaggtgga cagggactgg gcaatggact gctcatcccc gatgtcagag agcacccggc
      421 ggaagacccg caccgtgctg tccgacttgg cagggatgtg cagcccgcac tgggccatca
      481 gtgtaatgag gcccagggtc ttgagggtca ccgtcttgcc gccggtgttg ggaccggtga
      541 tgaccaaggt gtcgaaccgg tctcccagct ccaggtcgtt ggccaccgcc ttctttttat
      601 ccagcagggg gtgccgcgct ccccgcagcc tcaggtaccg ttccgacagg ccgggctggc
      661 tggcctccat cttcagggag agctgtcccc gggcgaagat ggtgtccagc aggatcagca
      721 gggtatagtc ctccccgata tcctccttat gggcggcgca ctgggcggac agctccgcca
      781 ggatgcgttc aatctccttt ttctccctgg ccgccagctc acgcagctcg ttattggcct
      841 tcaccacccc catgggctca atgaagaagg tggagccgct ggaggacacg tcgtggacca
      901 ggccggggat ggcgttcttg tgctccgcct tcaccggtac cacgtaccgg tcggagcgga
      961 tggtgatgat ggactcctgg aggtatttgg actggttgga ggagatcagc cgctggagga
     1021 tgtcccgcac cctggcctcg gtggagcgca tgtgccgccg gatggaggcc agctcgctgc
     1081 tggctgagtc ggcgatctcc tcctcactga ggatggagtt ggtgatgctg tcctccagga
     1141 agcggtttgg ggtcagggaa cggaacaggt gggagatgac ggtcttctcc tcgctgtccc
     1201 cgtagtcccg cgccccccgg gcgcaccgca gcacagaggc gatgtccagc agctcccggg
     1261 tagacaggct cccccccatg tccgcccgct ggaggctggc cgacaccggc ttcaccccgg
     1321 agaagccggg gctcccccgc agaatgagca tctttaccgc ggcggaggtc tcctcctgcg
     1381 cccgggccac gtcgtccaga tcagtgaggg gacgcagtgc caggcagcgc tcctttccct
     1441 ccagggtggc cgcgcagtcg gcaagaagag ccagcacctg gggcagctcc agggtggcca
     1501 tagatttttc aaacagttca ctcatataca aaactcctgg atcgttggtc acattccatt
     1561 ttggcggttc aaggaccgcc gtacacgttt tattgtctca taaaagcctc cggttgacaa
     1621 gccccgggcc tcgctccctg tattttttgc ccactcccgc cggccgagct ccacatacgc
     1681 ccgccctgtc accatggcgg cagggcgggc gtatgctatt tgacccggtc ccagc
//
LOCUS       DBMDSJ010000392         1732 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15208_length_1732_cov_1.019007, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000392 DBMDSJ010000000
VERSION     DBMDSJ010000392.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15208_length_1732_cov_1.019007"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            193..1314
                     /locus_tag="ACZPAQ_08505"
                     /pseudo
     CDS             193..1314
                     /locus_tag="ACZPAQ_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013347.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
     gene            1323..>1732
                     /locus_tag="ACZPAQ_08510"
     CDS             1323..>1732
                     /locus_tag="ACZPAQ_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904070.1"
                     /translation="MPYHIKKFRGYRRELKRVLRVVEEYQPNWCPAEEDFREEWLDLG
                     ANRFFHPRGKDRKALMAALLRKTEELIAVRPADLPFCKIFLFVPGGDPALAEIILIYD
                     PDRFQTFWVRTDPADQLWIPQEGPSLLAELGLESS"
BASE COUNT          332 a          486 c          580 g          334 t
ORIGIN      
        1 cgccaggtgg gactggagcg gatagggagc gtgtccttct atccccctat ggcgctttga
       61 cagggataaa cgtgggacag ggcgaagatc gccctgtccc ttttttacac cggcggcttg
      121 gagaaaaaga atttgccata gaccgtcaga tacggtatac tggacccaca aaagagagga
      181 gaggtgttgc ctgtgggcga gctgaatttt gctgtcccca ccctgtttgg cctggagggg
      241 ctgtgcgctg acgagatccg gaggctgggt ctgcccagtg tccaggcgga aaatggccgg
      301 gtgctgtgta ccggaacaga gggggacctg ccccggctga atctgaatct gcgtaccggg
      361 gagcgggttc tgctagtgct ggggacattc ccggctggaa actttgacgc tctatttgaa
      421 gggacgcggg ccctgccctg ggagcggttc atccctcggg agggccagtt tccagtgaag
      481 ggccactccc tcaactccgc cctccactcg gtgcccgcct gccaatccat cgtgaaaaag
      541 tcggtggccg cccggctggg agaggcttac ggcttgaata ccctgccgga aacaggggcg
      601 ctgtaccaga ttcagttcgc gattatgaag gataccgcca ccctgatgct ggatacctcc
      661 ggcgctgggc tgcacaagag gggctaccgg gcccaggggg tgattgcccc cctgcgggaa
      721 acgctggcgg cggccatggt cctgctgtcc cggtacagag gccgggaccc cctgtgcgac
      781 cccttctgcg gcagcggcac catccccatc gaggctgcct tgattgcccg aaaccgggcg
      841 ccggggctga acaggtcctt ctccgcccaa aaatgggggt ggctggacaa gcggctctag
      901 atggaggcgg ctgaccaggc catggaccag gaatttgatg gggattatga gatttggggc
      961 ggcgacctgg accccgccgc cgtggagctg gcccggcaca atgctcaact ggcagaggtg
     1021 gacgacatcg tatcctttga ggcggccgat gcccggacct tccactgggg cggcctctat
     1081 ggccgggtgg tcaccaatcc gccttacgga gagcgcatca tggagcgccg ggaggcggag
     1141 gagttgtacc gggccttcgg ccgagcctgg agcaaatttc cagagggctg gaagctcttc
     1201 cttttgtcct cccacaccga gtttgagcgg actttcggcc ggcaggcgga caagaagcga
     1261 aaactctata acgggatgct gaagtgcgac ctgtttatgt acgggatgaa ataggagggg
     1321 tcatgcctta ccatataaaa aaatttcggg gatatcggcg ggagctgaaa agggttctgc
     1381 gggtggtaga ggagtaccag cccaactggt gtccggcgga agaggatttt cgagaggagt
     1441 ggctggacct gggagccaac cgtttttttc atcccagagg gaaggaccgg aaggcgctga
     1501 tggccgccct cctgcgaaag acggaggaac tgattgccgt caggccggcg gacctgccct
     1561 tctgtaaaat attcctcttt gtccctgggg gcgacccggc tctggcggag atcatcctca
     1621 tctacgaccc ggaccgcttc cagaccttct gggtgcgtac cgacccggcg gaccaactgt
     1681 ggattcccca ggaaggtccc tccctgctgg cggagcttgg gctggagagc tc
//
LOCUS       DBMDSJ010000393         1729 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15234_length_1729_cov_1.444717, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000393 DBMDSJ010000000
VERSION     DBMDSJ010000393.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15234_length_1729_cov_1.444717"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1729)
                     /locus_tag="ACZPAQ_08515"
     CDS             complement(<1..>1729)
                     /locus_tag="ACZPAQ_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR0904071.1"
                     /translation="AAGLRCDESAMTGESVPVEKAAGGPLPEDAPLGDWTNMVISGTL
                     VTAGRATALVTATGMDTQMGRIAGLLLEGETQPTPLQRKMGEISRSLSFLCLSVCAVM
                     FGVGLLQGKDMLGMFLTAVSLAVAAIPEGLPAIVTIVLALGVGRMADRGAIVKKLPAV
                     ETLGCASVICSDKTGTLTQNRMTVRELWVPAGGHRRDALLAGALCGDARLEWKAGAPV
                     SAGDPTEGALVVAAAREGLDRNQLSESLPRTGEIPFDSGRKLMTTVHEKEGGGRLVLV
                     KGAPDVLLERCVSGPRGPLSPADRRQILAANEDMARRALRVIGVARRELSLQPRKLES
                     GTGERDLTFLGLFGMADPPRREVKGAVERCHQAGIRPVMITGDHRATAMAVARELDIC
                     RPGDLALTGPELDFMPQEMLEEDIEKFAVFARVSPEHKMRIVRAWQKKGRVVAMTGDG
                     VNDAPALKAADIGCAMGLSGTDVAKGAAEMILTDDNFSTIVQAVEEGRGIYSNIRKAI
                     HYLLSCNIGEIFTIFVATLLNFGQMPLVPVQLLWLNLVTDSLPALALGVEPVEEGVMD
                     QPPRDPSAGL"
BASE COUNT          248 a          659 c          557 g          265 t
ORIGIN      
        1 acagcccggc ggaggggtcc cggggcggct ggtccatcac gccctcctcc accggctcca
       61 cgcccagggc cagggcgggc agggagtcag tcaccagatt cagccacagc agctgcaccg
      121 gcaccagggg catctgcccg aagttcaaga gggtggccac gaaaatggtg aagatttccc
      181 cgatgttgca ggagagcaga tagtggatgg ccttgcggat attggagtag atcccccgcc
      241 cctcctccac cgcctggacg atggtggaaa agttgtcgtc tgtgaggatc atctccgccg
      301 ctcccttggc cacgtcggtg ccggacagcc ccatagcgca gccgatgtcc gccgccttga
      361 gggctggggc gtcgttgacg ccgtcccccg tcatggccac cacccgcccc tttttctgcc
      421 aggcccggac gatgcgcatc ttgtgctccg gcgacacccg ggcaaagaca gcgaattttt
      481 caatgtcctc ctccagcatc tcctggggca tgaagtccag ctccgggccg gtgagggcca
      541 ggtcccctgg gcggcagatg tccagctccc gtgccaccgc catggccgtg gcacggtggt
      601 ccccggtgat catcaccggg cggatgcccg cctggtggca ccgctccacc gcccccttca
      661 cctcccgccg gggtgggtcg gccatgccga acagccccag gaaggtaagg tcccgctccc
      721 ccgtcccgct ctccagcttt ctgggctgca gggacagctc ccgccgggcc accccgatga
      781 cccgcagggc ccggcgggcc atgtcctcat tggcggcgag gatctgtctc cggtccgccg
      841 gcgacagtgg gcctcttggg ccggacacgc accgctccag cagcacgtcc ggtgccccct
      901 tcaccagcac caggcggccg cccccctcct tttcgtggac ggtggtcatc agcttgcgcc
      961 cggagtcgaa gggaatctcc ccggtgcggg gcaggctctc ggacagctgg ttccggtcca
     1021 gcccctcccg ggcggcggcc accaccagcg ccccctcggt ggggtccccc gcggataccg
     1081 gggcccccgc cttccactcc agccgggcgt cgccgcacag ggcccccgcc agcagggcgt
     1141 cccgccggtg gccccccgcc gggacccaca gctcccgcac cgtcatccgg ttctgggtca
     1201 gggtgcccgt cttgtccgag cagatgacgc tggcacagcc cagggtctct accgcgggca
     1261 gcttcttcac gatggccccc cggtccgcca tccgccccac ccccagggcc agcacgatgg
     1321 tgacgatggc gggcaggccc tccgggatgg cggccaccgc cagggagacg gcggtgagga
     1381 acatgcccag catgtccttc ccctggagca gccccacccc gaacatgacg gcgcatacgg
     1441 agaggcacag gaaggagagg gagcgtgaga tctcccccat cttccgctgg aggggggtgg
     1501 gctgggtctc tccctccagc agcaggccgg cgatgcggcc catctgtgtg tccatgccgg
     1561 tggcggtgac cagggcggtg gcccgccccg ccgtgaccag tgtgccggag atcaccatgt
     1621 tggtccagtc tcccaggggg gcgtcctccg gcagcggccc acccgccgcc ttctccacgg
     1681 gcaccgactc cccagtcatt gcgctctcgt cacagcgcag tcccgccgc
//
LOCUS       DBMDSJ010000394         1724 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15286_length_1724_cov_1.622921, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000394 DBMDSJ010000000
VERSION     DBMDSJ010000394.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15286_length_1724_cov_1.622921"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="ACZPAQ_08520"
     CDS             complement(<1..1030)
                     /locus_tag="ACZPAQ_08520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR0904072.1"
                     /translation="METLNFAIPTLFGLEALAADELRRLGLDQVRAENGRVLCSGRPG
                     DIPRMDLNLRTGERVLLVLGTFPAGDFDALFEGTRALPWEQFIPREGRFPVKGHCLNS
                     ALHSVPACQSIVKKAAAARLGDAYGLNTLPETGALFQIQFSIMKDTAVLMLDTSGPGL
                     YKRGYRAHGVDAPLRETLAAAMVLLSRYRGRDPLCDPFCGSGTIPIEAALIAKNRAPG
                     LNRSFSAQKWSWLPAQAWMDAADEAQDREFHGDYEIWGGDIDPAAVELARHNAQLAEV
                     DDIVRFEAADARTFHWGGMYGRIVTNPPYGERIMERREAEELYRAFGKAWTKFPETWK
                     LYLLSSHTE"
     gene            complement(1042..>1724)
                     /locus_tag="ACZPAQ_08525"
     CDS             complement(1042..>1724)
                     /locus_tag="ACZPAQ_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015000.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR0904073.1"
                     /translation="QVQEKSSSSSRKPFALEHSYENGFRLLELDFSWTCDGELVCVHD
                     WNAFYGRILDTDSPTLAQFEAVRDSTYGFTSLTLDHLIAWLREHPGVSIVTDIKEDCA
                     AGAALIAQRCPDLRSRFIIQIYHPEEYDQVADLGFEHIILTVYQMSWSEKQDTAALVR
                     FAAEHPLEGITFPAELADTPGYVEALLEAQVPLFVHTVNDPADQAALVRQGISGIYTD
                     LGAPAPQE"
BASE COUNT          304 a          530 c          602 g          288 t
ORIGIN      
        1 actccgtatg ggaggagagc agatacagct tccaggtctc cgggaacttg gtccaagcct
       61 ttccgaaggc gcggtacagc tcctctgcct cccggcgctc cataatgcgc tcgccgtagg
      121 gaggattggt gacgatccgg ccgtacatcc cgccccagtg gaaggtgcgg gcgtccgccg
      181 cttcaaacct tacaatgtcg tccacctcgg ccagctgggc gttgtgccgg gccagctcca
      241 cggcggcagg gtcgatatcg ccgccccaga tctcatagtc cccgtggaac tcccggtcct
      301 gggcctcatc ggcggcatcc atccaggcct gagcggggag ccagctccac ttctgggcgg
      361 agaaggagcg gttcaggccc ggagcccggt ttttggcgat gagggcggcc tcgatgggga
      421 tggtgcccga accgcagaag gggtcgcaca gcgggtcccg gccccggtag cgggagagga
      481 gcaccatggc ggcggccagg gtctcccgca ggggggcgtc caccccgtgg gcccggtagc
      541 cccgcttgta aaggcctggt cccgaggtgt ccagcatcag gacggcggtg tctttcatga
      601 tggagaactg gatctggaag agcgctccgg tctcagggag ggtattcagg ccgtaggcat
      661 cccccagacg ggcggcggcc gcctttttca caatggattg gcaggcaggc acggaatgga
      721 gggcggaatt aaggcagtgc cccttgacag ggaaccggcc ctcccggggg atgaactgct
      781 cccaggggag ggcacgggtc ccctcaaaca gggcgtcgaa atctccggca gggaaggtgc
      841 ccagcaccag cagcacccgc tccccggtgc gcagatttag atccatccgc gggatgtcgc
      901 cgggccggcc ggagcagagg acgcggccat tttccgcccg cacctggtcc agccccaggc
      961 ggcgcagctc atccgcagcc agggcctcca ggccgaagag ggtggggatc gcaaagttca
     1021 gagtttccat ggcagttctc cttattcctg gggggcgggc gccccgagat cagtgtagat
     1081 gcccgagatg ccctggcgga ccagggcggc ctgatccgcc gggtcattga ccgtgtggac
     1141 gaagaggggg acctgggcct ccaggagcgc ctccacatag cctggggtgt ccgccagctc
     1201 cgccgggaag gtgatcccct ccaggggatg ttcagcagca aagcggacca gggcggcggt
     1261 gtcctgcttt tcggaccagg acatctgata cacggtgagg atgatgtgct caaagcccag
     1321 atctgccacc tggtcatatt cttccgggtg gtagatctga atgatgaagc ggctgcgcag
     1381 atccggacag cgctgggcga tcagggcggc gcccgccgcg cagtcctcct tgatgtcagt
     1441 gacgatggag acgccggggt gttccctcag ccaggcgatc agatgatcca gggtcaggga
     1501 agtgaagccg taggtgctgt cccggacggc ctcgaactgg gccagggtgg gggagtccgt
     1561 gtccaggatg cggccataga aagcgttcca gtcgtggacg cagaccagct ccccgtcgca
     1621 ggtccaggag aagtccagct ccagcagccg gaagccgttt tcgtaggaat gttccagcgc
     1681 aaacggcttc cggctgctgg agctggactt ctcctggacc tgcg
//
LOCUS       DBMDSJ010000395         1719 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15337_length_1719_cov_1.169345, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000395 DBMDSJ010000000
VERSION     DBMDSJ010000395.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15337_length_1719_cov_1.169345"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..1442
                     /gene="mnmE"
                     /locus_tag="ACZPAQ_08530"
     CDS             60..1442
                     /gene="mnmE"
                     /locus_tag="ACZPAQ_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR0904074.1"
                     /translation="MERTTDTIAAVATPAGPGAIGILRLSGPEAVSAASAAFRAADGR
                     PLEAHPPRRLIYGDLLDREGAVIDKVLATYTRGPASYTGEDTAELHCHGAPMVLSLGL
                     ESLFSHGARQARPGEFTRRAFLNGRLDLVQAEAVGDLLEASSREGARHAAGQLSGALS
                     RRIGGIYSALVDVMAHFHAVLDYPDEDIDPFRESELEVVLSRQAAQLRALLATCRRGS
                     QILHGLRCAIVGRPNAGKSSLLNALLGYERAIVTEIPGTTRDTVEETVTVGGTLLRLI
                     DTAGLRDTPDRVEQMGVERSRAAMESAELILVLWDSSSPVTQEDGELLCQATSLAPTV
                     LVRSKSDLLSAPLPLLSLDPMPPVVELSARTGQGLEELEQAIRSALPRQTGEEAYGEL
                     LTNARQEEAASRALERLEGAQAGLSAGVTPDALLTDVEEALSALGELTGASVREDITD
                     RIFQRFCVGK"
     gene            complement(1490..>1719)
                     /gene="lepB"
                     /locus_tag="ACZPAQ_08535"
     CDS             complement(1490..>1719)
                     /gene="lepB"
                     /locus_tag="ACZPAQ_08535"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR0904075.1"
                     /translation="QVLDEPYILEEMLWPSSSHMQETHFEVPEDSIFVMGDNRNGSTD
                     SRHEWLGAIDEDYILGQAVAVLWPLGRIGLL"
BASE COUNT          249 a          625 c          573 g          272 t
ORIGIN      
        1 tttgcaggaa ccgctgcgtt tcctgtcgaa gcgcgccgcc aggaaggagt atcgttctga
       61 tggagagaac caccgacacc attgccgccg tggccacccc cgccgggccg ggggctatcg
      121 gcatcctgcg tctgtccggg ccggaggccg tctccgccgc gtccgccgcc ttccgggcag
      181 cggacggccg gccgctggag gcccatcccc cccgccgcct gatctacggc gacctgctgg
      241 accgggaggg ggcggtcatc gacaaggtgc tggccaccta tacccggggc ccggcctcct
      301 acaccggtga ggacaccgct gagctccact gccacggcgc ccccatggtg ctctctctgg
      361 ggctggagtc cctcttctcc cacggggccc gacaggccag accgggggag ttcacccgcc
      421 gtgcctttct taacggccgt ctggatctgg tgcaggcgga ggcggtgggc gatctgctgg
      481 aagcctccag ccgggagggg gcccgccacg ccgccgggca gctgtccggg gccctgtccc
      541 ggcggatcgg cggcatctac tccgccctgg tagacgtgat ggcccacttc cacgcggtgc
      601 tggactaccc ggacgaggac atcgacccct tccgggagag cgaactggag gtggtcctgt
      661 cccggcaggc cgctcagctc cgggcccttc tggccacctg ccgccggggc agccagatcc
      721 tccacggcct gcggtgcgcc atcgtgggcc gccccaacgc gggcaagtcc tccctgctca
      781 acgccctgct gggctacgaa cgggccattg tcactgagat ccccggcacc acccgggaca
      841 ccgtggagga gaccgtcacc gtagggggca ccctgctgcg gctgatcgac actgccggcc
      901 tgcgggacac cccggaccgg gtagagcaga tgggcgtgga gcgcagccgg gctgccatgg
      961 agtcggcgga gctgatcctg gtgctttggg acagctcctc ccctgtgaca caggaggacg
     1021 gggagcttct gtgtcaggcc acgtctctgg ctcccaccgt gctggtgcgg agcaagtctg
     1081 acctcctctc cgcccccctc cccctcctgt ccctggaccc catgccccct gtggtggagc
     1141 tgagcgccag gacaggacag ggcctggagg agctggaaca ggccatccgc tccgcactgc
     1201 cccggcagac cggggaggag gcctatgggg agctgctcac caacgcccgc caggaggagg
     1261 cggcctcccg ggcgctggag cggctggagg gcgcccaggc tggactgtcc gccggcgtca
     1321 ccccggacgc cctgcttacc gacgtggagg aggccctctc cgccctgggg gagctcaccg
     1381 gcgcctccgt gcgggaggac atcaccgacc ggatcttcca gcgtttctgc gttggaaaat
     1441 aagctgagcg cgcggacctt ccggtccgcg cgctcagctt atttccccgt tacagcaggc
     1501 cgatccgccc cagcggccac agcaccgcca ccgcctgtcc caggatatag tcctcatcaa
     1561 tggcgcccag ccactcgtgc cggctgtcgg tggagccgtt gcggttgtct cccattacga
     1621 aaatggagtc ctccggcacc tcgaagtggg tctcctgcat gtggctggag gagggccaca
     1681 gcatctcctc cagaatatag ggctcgtcca gcacctgtc
//
LOCUS       DBMDSJ010000396         1717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15364_length_1717_cov_1.278465, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000396 DBMDSJ010000000
VERSION     DBMDSJ010000396.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15364_length_1717_cov_1.278465"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="ACZPAQ_08540"
     CDS             complement(<1..300)
                     /locus_tag="ACZPAQ_08540"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HIR0904076.1"
                     /translation="MPEHYITAGGKRLRCGRTTGTCAAMAAAGAVRLLLTGRAPDLIS
                     LNTPRDWMVEAPLLDCVADGETAACSVEKDGGDDVDATHGLPIRATVRRRAEGGTA"
     gene            complement(334..1101)
                     /locus_tag="ACZPAQ_08545"
     CDS             complement(334..1101)
                     /locus_tag="ACZPAQ_08545"
                     /inference="COORDINATES: protein motif:HMM:NF017944.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR0904077.1"
                     /translation="MREAILAVSFGSTVDQARSRDLEGVEQALSAAAPELPLFRAYTS
                     GLVRARLGDRGIAVPDVAGALEQMAAEGIECALIVPTHLLPGAEYDKIRETAARWEGR
                     FPALRLSRPLLGTPEDLRELGGALMDLIPPAEGAQTLFMGHGTHRSADLVYPALQGIF
                     HLAGRDDLVVATVEGRPRVEEVLPLVTAPAVELVPLMLVAGDHALHDMAGEGPDSWKR
                     LLQSRGHTVSCRLTGLGALPQVQALYVRRLEELRAGS"
     gene            complement(1098..>1717)
                     /locus_tag="ACZPAQ_08550"
     CDS             complement(1098..>1717)
                     /locus_tag="ACZPAQ_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0904078.1"
                     /translation="GRAFLMGEDLLAMSGREMAQRLAVVVQEHESGFDFTVEEVVRMG
                     RHARKGLLEADNGQDRSLVDRVLRLTQLEEVREQSYTTLSGGEKQRVLIARALVQDTP
                     CLILDEPTNHLDIKYQLQLMELVRVLGRTVVAAIHDLNLAALYCQRLYVMKAGRVAAS
                     GTPEEVLTPELLREIYEVEAEVARDSRGCLRIFFQPIRNEGEESI"
BASE COUNT          237 a          666 c          549 g          265 t
ORIGIN      
        1 ggccgtcccc ccctccgccc tgcggcggac cgtggcccgg atgggcagcc cgtgggtggc
       61 gtccacgtca tctcccccgt ccttctccac ggagcaggcg gcggtctctc cgtctgcgac
      121 gcagtccagc aggggggcct ccaccatcca gtctctgggg gtgttcaggg agatcaggtc
      181 cggggcccgc cctgtgagga gcagccgcac cgcccccgcc gccgccatgg cggcgcaggt
      241 cccggtggtg cgtccgcagc gcaggcgctt cccgcccgca gtgatgtagt gctccggcat
      301 cggcagctcc tttcccgcgg catgtctctt ctctcaggac ccggcccgaa gctcctccag
      361 gcggcggaca tagagggcct gtacctgggg cagggccccc agtccggtga gccggcagga
      421 cacggtatgc ccccggctct ggaggagcct tttccagctg tccggcccct cccccgccat
      481 gtcgtggagg gcgtgatccc cggccaccag catcagcggc accagctcca cggcgggggc
      541 ggtgaccagc ggcagcacct cctccaccct gggccggccc tccaccgtgg ccaccaccag
      601 gtcgtcccgc ccggccagat ggaagatgcc ctggagggcc gggtagacca ggtcggcgga
      661 tctgtgggtc ccgtggccca tgaacagggt ctgggccccc tctgccgggg ggatcaggtc
      721 catcagcgcc ccgcccagct cccgcaggtc ctccggcgtc cccagcaggg ggcgggagag
      781 cctcagcgcg ggaaagcggc cctcccaccg ggcggcggtt tcccttattt tatcatattc
      841 cgcgcccggg agcaaatggg tgggcacgat cagggcgcac tctatcccct ccgccgccat
      901 ctgctccagg gcaccggcca catccgggac cgcgatcccc cggtccccca gccgcgcccg
      961 gaccagaccg ctggtgtagg cccggaagag gggcagctcc ggggcggcgg cggacagcgc
     1021 ctgctccacc ccctccaggt cccggctccg ggcctggtcc accgtgctgc cgaagctgac
     1081 ggccagaatg gcttctctca tatcgattcc tccccttcgt ttcggatggg ctgaaaaaag
     1141 atgcgcaggc agccccggct gtcccgcgcc acctccgcct ccacctcata gatctcccgg
     1201 aggagctccg gggtcagcac ctcctccggt gtgcccgagg cggccacgcg gccagccttc
     1261 atcacataga gccgctggca gtacagggcc gccagattca ggtcgtggat ggccgccacc
     1321 accgtccggc ccaggacccg caccagctcc atcagctgga gctgatattt gatgtccaga
     1381 tggttggtgg gctcgtccag gatcaggcag ggggtgtcct ggaccagggc ccgggcgatg
     1441 agtacccgct gcttctcccc gccggacagg gtggtgtagc tctgctcccg cacctcctcc
     1501 agctgggtga ggcggagcac ccggtccacc agcgaccggt cctgcccatt gtcggcctcc
     1561 agcagtccct tccgggcgtg ccgccccatg cggaccacct cctccaccgt aaagtcgaag
     1621 ccgctctcgt gctcctggac caccacggcc agccgctggg ccatctcccg gccgctcatg
     1681 gccagcaggt cctcccccat aaggaatgcc cgccccg
//
LOCUS       DBMDSJ010000397         1715 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15386_length_1715_cov_1.078686, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000397 DBMDSJ010000000
VERSION     DBMDSJ010000397.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15386_length_1715_cov_1.078686"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="accD"
                     /locus_tag="ACZPAQ_08555"
     CDS             complement(<1..768)
                     /gene="accD"
                     /locus_tag="ACZPAQ_08555"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR0904079.1"
                     /translation="MNNIFAQRRSRLLAMKALRERYVPNEKAMTCPKCSQISERQAVS
                     QNLFVCPLCGYHFPIGAYYRLSTILDSGSFRELFEKLPAGDPLSFPGYKSKLEESRRK
                     TGLNEAAVIATGTVDGRKCVVGVLDSRFLMGSMSAAVGEKITLGIEYATKNKLPLILF
                     AASGGARMQEGILSLMQMAKTSAALARFSQKGLLYISILTDPTTGGVTASFASLGDII
                     LAEPGALIGFAGPRVIQQTIGQTLPEGFQRAEYQEEHG"
     gene            complement(765..>1715)
                     /locus_tag="ACZPAQ_08560"
     CDS             complement(765..>1715)
                     /locus_tag="ACZPAQ_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0904080.1"
                     /translation="AERVGYPVLLKASAGGGGRGIRRCNSPEELPAAFQAAKAEARAC
                     FGDDQMYLEKLILRPRHIEFQILADCQGNVIHLGDRDCSVQRRNQKLIEEAPAWCLTP
                     ELRAEMGAAAVRAAQAVGYENAGTVEFLLDSDGTHFYFMEMNTRIQVEHGITELITGV
                     DLVRQQLRIASGLPLDVTQEEVCLTGHSIECRINAEDPGKNFQPCPGTVEFLHFPGGP
                     GIRVDSCLYNGCTLSPYYDSMAAKLLVHAPTRLEAIKRMRRCLEEFALEGFATNAELS
                     YQILYHPAFVRGSCTTAFLDENLAELLDFSRRLEESEGAQ"
BASE COUNT          335 a          526 c          542 g          312 t
ORIGIN      
        1 gccatgctcc tcctggtact ccgcccgttg gaagccctca ggcagagttt ggccaatggt
       61 ctgctggata acccggggac cggcaaagcc aatgagggct ccgggctccg ccagaatgat
      121 gtcccccagg gaggcaaagc tggcggtgac cccgccggtg gtggggtcgg tgaggatgga
      181 gatataaagc aggccctttt gtgaaaagcg agccagagcc gcgctagtct tggccatctg
      241 catcagagac aggatgccct cctgcatccg agcgccgccg ctggcggcaa agaggatgag
      301 gggcagcttg tttttagttg catactcgat gcccaaagtg atcttttctc ccacagcggc
      361 gctcatggag cccatcagaa accggctgtc caacaccccc accacgcact tgcggccatc
      421 cacagtgccg gtggcgatga cggcagcttc attgaggccg gttttgcgcc ggctctcctc
      481 cagcttggac ttgtagccgg ggaaggataa gggatcgccg gcgggcagct tttcaaacag
      541 ctcccgaaag gagccggagt ccaaaatggt gctgaggcgg taataggctc cgatggggaa
      601 gtggtagccg cacagaggac agacaaagag attctgggac acggcctgcc gctcgctgat
      661 ctgggaacat ttggggcagg tcatggcctt ctcattgggc acatagcgtt cccgcagggc
      721 tttcatggcc agcaggcggg accggcgctg ggcaaagatg ttgttcactg ggccccctca
      781 ctttcttcca gccggcggct gaagtccagc agctcggcca gattctcgtc taaaaacgct
      841 gtggtgcagc ttccccgaac aaaggccggg tggtacagga tctgatagga cagctccgcg
      901 ttggtggcaa agccctccag ggcaaactcc tccagacagc ggcgcatccg cttgattgcc
      961 tccagccggg tgggggcgtg gaccagcagc ttggccgcca tggagtcgta gtagggagac
     1021 agggtacatc cgttatacag gcaggagtcc accctgatgc ccggcccgcc aggaaagtgg
     1081 agaaactcca cagttccggg acagggctga aagtttttcc cagggtcctc agcgttaatg
     1141 cggcattcga tggaatgccc ggtgaggcac acctcctcct gggtcacatc caggggcagg
     1201 ccggaggcga ttcgcagctg ctgacgaacc aggtctaccc cggtgatcag ctcagtgatg
     1261 ccgtgctcca cctggatgcg ggtgttcatc tccataaaat aaaagtgagt cccgtccgag
     1321 tccagcagaa attccaccgt acccgcgttc tcatagccca ccgcctgggc ggcccggacg
     1381 gcggcggctc ccatctctgc ccgcagttca ggggtgaggc accaggcggg ggcctcctcg
     1441 atcagcttct ggttgcggcg ctgaaccgag cagtcccggt cccccaagtg gatcacattt
     1501 ccctgacagt cagccaggat ctggaactca atgtggcggg gccgcaggat cagcttttcc
     1561 agatacatct ggtcgtcgcc gaagcaggcc cgggcctccg ccttggcagc ctgaaaggcg
     1621 gcgggcagct cctccggaga gttgcatcgg cggatgcccc ggccgccccc gccagcggag
     1681 gccttcagca gtaccgggta gccaacccgc tccgc
//
LOCUS       DBMDSJ010000398         1714 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15402_length_1714_cov_1.066956, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000398 DBMDSJ010000000
VERSION     DBMDSJ010000398.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15402_length_1714_cov_1.066956"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="ACZPAQ_08565"
     CDS             complement(<1..1113)
                     /locus_tag="ACZPAQ_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit domain-containing
                     protein"
                     /protein_id="HIR0904081.1"
                     /translation="MIQYDKAAGQFVCDFVERLPTTDTGKPFRLYRWQRETLMEFYST
                     MELDSESERWLRRYQYLYLEIPKKNGKSELSAALGIYHLFGDGELNAEVYICAADKEN
                     AGIVFRAAVFMLETAPWTAKMIARGELKIIRSQKKIEYRRQVKAENGGLRWVSVGLMQ
                     VLSSESYSKHGYKPSCVIFDELHAQPDRKLWDVMTGAAGAAHTQPVWLVLTTAGDDPD
                     RGSIGWEIHEKAVAIRDARRLRTIEAEGGDPRQILSLRHTADEDLERAKTALLAKDES
                     NWLPVLYGLTAMFGDDPDDLDQVDIWDEALWYQCNPSLGEHLTLRALRLEAQAAKKSE
                     AAEKLFRWLRLNQWISTKAVSWIPLTLYDKTQWNRPE"
     gene            complement(1110..1619)
                     /locus_tag="ACZPAQ_08570"
     CDS             complement(1110..1619)
                     /locus_tag="ACZPAQ_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HIR0904082.1"
                     /translation="MAGKRQPTDIVKANGRQHLSQAEEDTRRDREIHVPSPEQAVPPR
                     WLGRKFHQEFREIGEILRTAGLYTELDRDVLGQFLVARERWVRADRLASDAIRKKDEK
                     LAREWAGVQGTYFKQCRQCAEAMGLSITSRCRLVVPEVMVNAARTEGEEDEFTRLLKQ
                     RQEAALAQA"
BASE COUNT          249 a          601 c          506 g          358 t
ORIGIN      
        1 ctccggccgg ttccattggg tcttgtcata cagggtcaga ggaatccagc tcaccgcctt
       61 ggtggagatc cattggttga gccgcagcca gcggaacagc ttttccgccg cttcgctctt
      121 tttggccgcc tgagcctcca gccgcagagc ccgcagggtc aggtgttcgc ccagggaggg
      181 gttgcactgg taccacagtg cctcgtccca gatgtccacc tgatccaggt cgtccgggtc
      241 gtccccgaac atggcggtca gtccgtacag cacgggaagc cagttgctct cgtctttggc
      301 cagcagagcg gtcttggccc gctccaggtc ctcgtcagcg gtatgccgca gggacaggat
      361 ctgcctgggg tccccgccct cggcctcgat ggtcctcagc cgccgggcgt cccggatggc
      421 cacggctttc tcatggatct cccagccgat gctgccccgg tccgggtcgt cgccggcggt
      481 ggtcagcacc agccacacag gctgggtgtg ggcggcgccg gccgcgccgg tcatcacgtc
      541 ccacagcttc cggtccggct gggcgtggag ctcgtcgaag attacgcagc tgggcttgta
      601 gccgtgcttg gagtaggact ccgaggagag cacctgcatc agccccacgc tgacccagcg
      661 caggccgccg ttctccgcct tcacctgccg ccggtattcg atctttttct gggaccgaat
      721 gatcttcagc tctccgcggg cgatcatctt ggccgtccag ggggcggtct ccagcatgaa
      781 taccgccgcc cggaacacaa tgccggcgtt ttccttgtcc gccgcgcaga tatagacctc
      841 cgcgttcagt tctccatccc caaacaggtg atagatcccc agagcggcgg acagttcgct
      901 cttgccgttc ttcttgggga tctccaggta gaggtactgg taccgcctca gccaccgctc
      961 cgactcgctg tccagttcca ttgtggaata gaactccatc agggtctccc gctgccatcg
     1021 gtagaggcgg aagggcttgc ccgtgtctgt ggtaggcagg cgctccacaa agtcacagac
     1081 aaactgcccg gcggccttgt cgtactggat catgcttggg ccagcgccgc ctcctgacgt
     1141 tgcttcagga gccgggtaaa ctcatcctcc tcgccctcgg tcctggctgc gttgaccatc
     1201 acctccggta ccaccaggcg gcatcgtgag gtgatggaca gtcccatagc ctcggcgcac
     1261 tggcggcact gcttgaagta ggtcccctgc acacctgccc attcccgggc cagcttctcg
     1321 tctttcttcc ggatggcgtc agaggccagc ctgtcggccc gtacccagcg ttccctggcc
     1381 accaggaact ggcccagcac atcccggtcc agctccgtat acagcccggc cgtccgcagg
     1441 atctcgccga tctcccggaa ctcctggtgg aatttcctgc ccagccaccg gggcggcacc
     1501 gcctgctccg gcgaggggac gtggatctca cggtcccggc gggtgtcctc ctccgcctgg
     1561 ctcagatgct ggcggccgtt ggccttcacg atgtcagtgg gctgccgctt tccagccatt
     1621 ttcctcactc ccttgtcggt gtccaacttg gacacattct ccgctggccg gagtctccct
     1681 ctgcctgccg ttcccctggg gtgtctctcc caaa
//
LOCUS       DBMDSJ010000399         1708 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15446_length_1708_cov_1.843186, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000399 DBMDSJ010000000
VERSION     DBMDSJ010000399.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15446_length_1708_cov_1.843186"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="ACZPAQ_08575"
     CDS             complement(<1..648)
                     /locus_tag="ACZPAQ_08575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR0904083.1"
                     /translation="MKNKMLRNGVEMPEVGFGTWKAGETDGFAVLSEAIRAGYRHIDT
                     ASAYHTEEAVGRAVAASGVDRSEFFITTKAWKDQLGYDRTLAAFDASCQALGMDYLDL
                     YLIHWPRPDAACEEWKELDRGSWRAMEELYRAGRVRAIGVSNFLPHHLENLLEVCTVP
                     PMVDQIEFHPGYVQWETVEFCRASQILVEAWSPLGRQRLADDPLLAELAGKYGVSQ"
     gene            complement(650..1312)
                     /locus_tag="ACZPAQ_08580"
     CDS             complement(650..1312)
                     /locus_tag="ACZPAQ_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR0904084.1"
                     /translation="MSWSREKQELRRKICQAARQIAQAGYVAANDGNLSARCSDGGVL
                     ITPSGVYKGDLEEDMLLEVDLEGRVLSGTGRPSSESPMHLALYRTRPEVGGVVHTHAP
                     YSVFSANLGEDLTEPITADWALLLGPVPALPWLPLGTEELAGAVARAAEGHSAALLAH
                     HGAVTWGRDVVQAWHRTQALEQYCKQRYLGLLLGRPIPVLEPEEVRELTVRRLAGEER
                     RN"
     gene            complement(1309..>1708)
                     /gene="mtnA"
                     /locus_tag="ACZPAQ_08585"
     CDS             complement(1309..>1708)
                     /gene="mtnA"
                     /locus_tag="ACZPAQ_08585"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312608.1"
                     /note="isomerizes methylthioribose-1-phosphate into
                     methylthioribulose-1-phosphate; involved in methionine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR0904085.1"
                     /translation="MVMKNGWVQACFVGCDRVAANGDTANKIGTSGLAVLARYYNVPF
                     YVLGPTSTIDLDCPTGADIPIEERDGEEIRSLWYREPMAPAEVRCWDPAFDVTDHSLI
                     SAIVTEGGICRPPFGESLRELVSREKGDAP"
BASE COUNT          272 a          635 c          513 g          288 t
ORIGIN      
        1 ctgggagacc ccgtatttcc cggccagctc cgccagcagg ggatcatccg ccagcctctg
       61 gcggcccagg gggctccatg cctccaccag gatctggctg gcccggcaga actccaccgt
      121 ctcccactgg acatagccgg gatgaaactc gatctggtcc accatggggg gcactgtgca
      181 cacctccagc aggttctcca ggtggtgagg caggaagttg ctcaccccga tggcccggac
      241 ccggccggcc cggtacagct cctccatggc ccgccagctc ccccggtcca gctccttcca
      301 ctcctcgcag gcggcgtccg gcctgggcca atggatcagg tacaggtcca aatagtccat
      361 ccccagcgcc tggcatgagg cgtcaaaggc ggccagggtc ctgtcgtagc ccagctggtc
      421 cttccacgcc ttagtggtga tgaagaactc cgaccggtcc accccggaag cggccaccgc
      481 cctgcccacc gcctcctctg tgtggtaggc ggaggcggtg tcaatatgcc ggtagccagc
      541 ccggatggcc tcagagagca ccgcgaagcc gtcggtctcc cctgccttcc aggtcccgaa
      601 gcccacctcg ggcatctcca caccattgcg cagcatcttg tttttcatat caatttctcc
      661 tttcttctcc cgccagacgg cggaccgtca gttccctgac ctcctccggc tccaggaccg
      721 ggataggccg tcccagcagc aggcccagat accgctgctt gcagtactgc tccagggcct
      781 gggtccggtg ccatgcctgg accacgtccc gtccccaggt caccgccccg tggtgggcca
      841 gcagggcggc gctgtgcccc tccgccgccc gggccaccgc ccccgccagc tcctctgtcc
      901 ccaggggcag ccagggcagg gcggggaccg gccccagcag cagggcccag tccgcagtga
      961 tgggctcggt caggtcctcc cccaggttgg ccgaaaacac ggaatatgga gcgtgggtgt
     1021 ggactacgcc tcccacctca ggccgggtac ggtagagagc cagatgcatc gggctctcgg
     1081 acgagggcct ccctgttccg gacagcaccc gcccctccag atccacttcc agcagcatgt
     1141 cctcctccag gtctccctta tagacccccg agggagtaat gagcaccccg ccatcggagc
     1201 accgggcaga caggttcccg tcgttggccg ccacatagcc cgcctgggct atctggcggg
     1261 cggcctggca gatcttccgc cgcagctcct gcttctctcg gctccagctc acggcgcatc
     1321 ccccttttcc cgggaaacca gctcccgcag gctctccccg aaggggggac ggcagatgcc
     1381 tccctccgtg acgatggcag agatcaggct gtggtcggtg acgtcgaagg cggggtccca
     1441 gcacctcacc tccgctggcg ccatgggctc ccggtaccag agggagcgga tctcttcccc
     1501 gtcccgctcc tcgatgggga tgtccgcccc ggtgggacag tccaagtcga tagtggaggt
     1561 agggcccagc acatagaagg ggacattgta gtaccgggcc aggaccgcca ggccgctggt
     1621 accgatcttg ttggcagtgt ccccgttggc cgccacccgg tcgcagccta cgaagcaggc
     1681 ctggacccag ccgttcttca tcaccatg
//
LOCUS       DBMDSJ010000400         1704 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15501_length_1704_cov_1.127261, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000400 DBMDSJ010000000
VERSION     DBMDSJ010000400.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15501_length_1704_cov_1.127261"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="ACZPAQ_08590"
     CDS             <1..256
                     /locus_tag="ACZPAQ_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR0904086.1"
                     /translation="EVWRLEENLYPFVELVRGVLSDQPLFVILNSYTTGLAPSVVTYI
                     LETLLSRKFGGRTDSQELGLPVTESGLVLPCGATGRWIRS"
     gene            366..1238
                     /locus_tag="ACZPAQ_08595"
     CDS             366..1238
                     /locus_tag="ACZPAQ_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR0904087.1"
                     /translation="MKDAIIRAEALRFSYTSAEGVAPVVLDGVDLSIQEGTFVAVLGH
                     NGSGKSTLAKHMNAILLPSGGKVYVDGIDTVDEDRLLDIRRTVGMVFQNPDNQIVANV
                     VEEDVAFAPENLGVPPAEIRERVDDALRAVGMYEFREYAPHLLSGGQKQRVAIAGVIA
                     MQPRCIVLDEPTAMLDPIGRSEVLRTIRDLNRKKGVTVVLITHHMDEAAQADRLVVMT
                     KGRVIADGPPRQVFQDVEGLRSVGLTVPDTTQLLWELRQEGLDVPLDALSDEECAQAL
                     CQLLNAASKKGSQP"
     gene            1235..1495
                     /locus_tag="ACZPAQ_08600"
     CDS             1235..1495
                     /locus_tag="ACZPAQ_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904088.1"
                     /translation="MNSTERSTRVFAGIAAFLVVFSAPRLLLEMFDDSFPTVLLGLTA
                     LAAAWAVVCLVSLRQRVKRLEMQARHLQAQLDDLAKQTGVLP"
BASE COUNT          305 a          549 c          539 g          311 t
ORIGIN      
        1 cgaggtgtgg cggctggagg agaacctcta cccctttgtg gagctggtgc ggggggtgct
       61 gtccgaccag cccctgtttg tgatcctcaa ctcctatacc accggtctgg cccccagcgt
      121 agtcacctat atcctggaga ccctgctctc ccggaaattc ggcggccgca ccgacagcca
      181 ggagctgggc ctccccgtca ccgagagcgg cctggtcctc ccctgcggcg ccaccgggcg
      241 ctggatcaga tcttgaccag ttaggagtta tgggctcggc cgttcactgc cgccctctcc
      301 agctcccgaa aaaaattctc aattgccaac ttctcattcc tgactcaaaa caaggggtgt
      361 tttgattgaa agacgcgatc atccgcgcgg aagctctccg cttctcctac acctccgccg
      421 agggcgtcgc ccccgttgtg ctggacgggg tggacctgtc catccaggag ggcacttttg
      481 tggctgttct gggccacaac ggcagcggca aatccaccct cgccaagcac atgaacgcca
      541 tcctcctgcc cagcggcggc aaagtgtatg tggatggcat cgacactgtg gatgaggacc
      601 gcctgctgga catccggcgc acggtgggca tggtgttcca gaacccggac aaccagatcg
      661 ttgccaatgt ggtggaggag gatgtagcct tcgcaccgga gaacctgggg gtcccccccg
      721 ccgagatccg ggagcgggtg gacgacgccc tgcgtgcggt ggggatgtat gagttccggg
      781 agtacgctcc ccatctcctc tccggcggtc agaagcagcg ggtggccatc gccggggtca
      841 tcgccatgca gccccggtgc attgtgctgg acgagcccac cgccatgctg gaccccatcg
      901 gccgctccga ggtgctgcgc accatccggg atctgaaccg gaagaagggg gtcaccgtgg
      961 tcctgatcac ccaccacatg gatgaggccg cccaggccga ccggctggtg gtcatgacca
     1021 agggcagggt cattgccgac ggccccccgc ggcaggtgtt ccaggacgtg gagggcctgc
     1081 gctccgtggg gctcactgtc cctgacacca ctcagctgct gtgggaactg cggcaggagg
     1141 ggctggacgt ccccctggac gccctcagcg acgaggagtg cgcccaggct ctctgtcagc
     1201 tgctgaacgc tgcatcaaag aaaggaagcc agccatgaac agtacagaga gaagcacccg
     1261 ggtctttgcg ggcatcgccg catttctggt ggtcttttct gcccccaggc tgctgctgga
     1321 gatgtttgat gacagctttc ccacggtact gctgggactg accgctctgg ccgccgcgtg
     1381 ggcggtcgtg tgtctggtct ccctccggca gcgggtcaag cggctggaga tgcaggcccg
     1441 ccatctccag gcccagctgg atgatctggc aaaacagacc ggcgtcctgc cgtaactgtg
     1501 atattttcgg gcgcgacccc gcggggcgtg caccacttca ctgaaaaagg atggaacaca
     1561 acggatggaa gcaattctgg agaccaaaaa gctctcccac atttacagcg ccggcacccc
     1621 cttcgagcgg ggggcgctgc tggaagtgga cttcaccgcc tacgccgggg aatacctggg
     1681 catcatcggc cacaccggct cggg
//
LOCUS       DBMDSJ010000401         1703 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15508_length_1703_cov_1.460674, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000401 DBMDSJ010000000
VERSION     DBMDSJ010000401.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15508_length_1703_cov_1.460674"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="ACZPAQ_08605"
     CDS             <1..494
                     /locus_tag="ACZPAQ_08605"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR0904089.1"
                     /translation="RDPRDYDARAGIMWAGSLSHNSLTGCGRGGRGRIGDWASHQLEH
                     ELSGMFDVTHGAGLAAVWGAWSRYVMDAAPERFARFARAVMGVDTPGSDEETALAGIR
                     AFEHFLRSIGMPTTVGQLGITLDEQAIEKLAWMCTFEGRRTIGSFKVLGLEEIKDIYR
                     AAR"
     gene            complement(606..>1703)
                     /locus_tag="ACZPAQ_08610"
     CDS             complement(606..>1703)
                     /locus_tag="ACZPAQ_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HIR0904090.1"
                     /translation="TFTMPYQGSTVEGRFFGNEAALSKVPAEWARELVRRLPELDRRG
                     LTFLSARAGLGTGGLALERFTIQMDRRLCLFYQRGEEQQMVRFAPDFALCEEDQPALA
                     QPEGGTFLAFVLLEQPEWDAEEFKRTLRDDWGIPCMTEEKDSGEGGSTLVFEAEGFLT
                     AVSLYPFPVPKGEAEQNAGRNYLWSEAEETTRRHRGQILVSTMARDGDVGQAARLQVK
                     LVCAACGQDGVLGIYANGTVYQPEFYLEAAQMMEDGSLPLLNLVWPGLYRREGGLCAY
                     TEGLRAFGKDELEVLDTRAEPEDLRGFVLDIASYVLEQDVTLRDGETIGFSEGQKLPI
                     TRSAGIWHDGMTLKIGYPSKEEREDENSGET"
BASE COUNT          258 a          624 c          525 g          296 t
ORIGIN      
        1 tccgggaccc ccgggactat gatgcccgtg ccggcatcat gtgggcgggg agcctgtccc
       61 ataacagcct caccggctgc ggccggggcg gccgcggccg catcggggac tgggccagcc
      121 accagctgga gcacgagctg agcggcatgt tcgacgtcac ccacggggcc ggtctggccg
      181 ccgtctgggg ggcctggtcc cggtatgtca tggacgcggc cccggagcgg ttcgcccgct
      241 tcgcccgggc ggtgatgggg gtggatactc ccggctccga cgaggagacc gcgctggccg
      301 gcatccgggc ctttgagcac ttcctccgct ccatcggcat gcccaccacc gtggggcagc
      361 tgggcatcac gctggacgag caggccatcg aaaagctggc ctggatgtgt acctttgagg
      421 gcaggcgcac cattggctcc tttaaggtgc tgggcctgga ggagatcaag gatatctacc
      481 gggcggcccg ataagctgtt catgaaaaac gccgtggcgt atcgtttccg atacgccacg
      541 gtgtttttat tgagtggatg aaatcagact tccccggcgg cttccagcgg tccgcaggtc
      601 atgtttcagg tctctccgct gttctcgtcc tccctctcct cctttgacgg atatccgatc
      661 ttcagcgtca tgccgtcgtg ccagatcccc gcgctgcggg tgatgggcag cttctgcccc
      721 tcgctgaagc cgatggtctc cccgtcccgg agggtgacat cctgctccag cacataggag
      781 gcgatgtcca gcacaaaccc ccgcaggtcc tcgggctccg cccgggtgtc cagcacctcc
      841 agctcgtcct tgccgaaggc gcgcagcccc tcggtgtagg cgcacaggcc tccctcccgg
      901 cggtacagcc ccggccacac caaattcagc aggggaaggc tcccgtcctc catcatctgg
      961 gccgcctcca gatagaactc cggctggtac acggtcccat tggcgtagat gcccagcacg
     1021 ccgtcctgtc cacaggcagc gcacaccagc ttcacctgga ggcgggcggc ctgccccaca
     1081 tctccgtccc gtgccatggt ggacaccagg atctgccccc ggtgccggcg tgtggtctcc
     1141 tccgcctcgc tccacaggta gttgcggccc gcattctgct ccgcctcccc tttgggcacg
     1201 gggaaggggt agaggctcac cgccgtcaga aagccctccg cctcaaagac cagggtgctt
     1261 cccccctccc cgctgtcctt ctcctccgtc atgcagggga tgccccagtc gtcccgcagg
     1321 gtccgcttaa attcctccgc gtcccactcc ggctgctcca gcagcacgaa ggccaggaag
     1381 gtccccccct caggctgcgc cagagcgggc tggtcctcct cgcacagggc aaagtcgggg
     1441 gcgaaccgga ccatctgctg ctcctccccc cgctggtaga acaggcagag ccgccggtcc
     1501 atctggatgg tgaaccgctc cagggccagt cccccagtcc ccagccctgc ccgggctgag
     1561 aggaaggtca gaccccggcg gtccagctcc ggcaggcgcc ggaccagctc ccgcgcccac
     1621 tcggccggca ccttggacag ggcggcctcg ttcccgaaga accgcccctc caccgtgctc
     1681 ccctgatagg gcatggtgaa cgt
//
LOCUS       DBMDSJ010000402         1689 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15638_length_1689_cov_1.755668, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000402 DBMDSJ010000000
VERSION     DBMDSJ010000402.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15638_length_1689_cov_1.755668"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="ACZPAQ_08615"
     CDS             <1..875
                     /locus_tag="ACZPAQ_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR0904091.1"
                     /translation="PDRPGGREMGGYLLLSYGTAWGIWLMCGMMPPGAVTAQLLNTVV
                     MWTPALAVWLMWLAGRRRPVLSFSLRPALRRNWKWYLAAWALSLAAAVLGALLYFGVY
                     PRDFDSTLPALAPMMEQAGISRGTAVSLLLGVLVYGPVINLFFAIGEEIGWRGFLYPA
                     LRQRMSTLPACLLVGAIWGLWHTPINLMGHNYGTSYPGFPWLGILGMCLFTTSAGTFL
                     AWLSERSGSLWPAALAHGTINAAAQVALLFRGAEGAVRQIWGPAVTGILAGMPMLVLS
                     AVLLVRKGSWRKSW"
     gene            complement(948..>1689)
                     /locus_tag="ACZPAQ_08620"
     CDS             complement(948..>1689)
                     /locus_tag="ACZPAQ_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR0904092.1"
                     /translation="LRAYQEAHYRPGNLVTALTGSFTPQAVEELRAILSELEPGHVPS
                     CKGARYRPAFTVRRKPTEQNHLILAFPAFSYLDPRRYQLLLLNSILGGGVSSRLFQEL
                     REKRGLCYTTYSYVADHADTGLLGVYTALSPELEGEALDAACALLRELAEHGPTQEEL
                     ERAREQAKASVLMGLESIQARMSHLGTSQLLYGSVLEQDDILDAYDAVTVPQLRQLAE
                     QIFDFSQLSLSAVGRVPPAEEYAQLLSP"
BASE COUNT          260 a          495 c          620 g          314 t
ORIGIN      
        1 tgcccgatcg gcccgggggc agggagatgg ggggatatct gctcctctcc tatgggacgg
       61 cgtgggggat ctggctgatg tgtggaatga tgcccccggg cgctgtcact gctcagctgc
      121 tcaacacggt ggtgatgtgg acgcctgcct tggcggtgtg gctgatgtgg ctggcgggga
      181 ggcgcagacc ggtgctgtct ttctccctgc ggcccgccct gagaaggaac tggaagtggt
      241 atctggctgc ctgggcccta tccctggctg ccgcagtcct gggtgcgctg ctctattttg
      301 gcgtatatcc ccgggatttt gacagcacgc ttccagcctt ggcgccaatg atggaacagg
      361 cggggatctc taggggcacg gcggtatccc tgctgctggg ggtactggtc tacggtccgg
      421 tgatcaatct gttctttgcc ataggagagg agatcggctg gcgggggttt ctctatccgg
      481 ccctgcggca gaggatgtcc accctccctg cctgcctgct ggtgggcgcg atttggggtt
      541 tgtggcacac accgatcaat cttatgggac acaactatgg caccagctat cccggcttcc
      601 catggctggg gattctgggg atgtgcctct ttaccacctc ggcaggcact ttcctggcct
      661 ggctgtcgga gaggagcggt tccctctggc cggcggcgct ggcccacggg acgatcaacg
      721 ccgccgcaca ggtcgctctg ctgttccggg gggcggaagg ggcagtgcgc cagatctggg
      781 gtcctgccgt caccggcata ctggcgggga tgcccatgct ggtcctgtcg gctgtccttc
      841 tggtgcggaa gggaagctgg cggaaaagct ggtaaaatcg taaaagcccg gggcggcact
      901 gatgtgccgc cccgggcttt ggtctgagac agaactggga gggagagtca gggggaaagc
      961 agctgggcgt actcctccgc cgggggcacc cggcccaccg cggacagaga gagctgggag
     1021 aagtcaaaga tctgctccgc cagctgccgc agctgaggga cggtgaccgc gtcataggca
     1081 tccaggatgt cgtcctgctc cagcacagag ccatagagca gctgggaggt gcccaggtgg
     1141 ctcatgcggg cctgaatgga ttccagcccc atgagcacgc tggccttggc ctgctcccgg
     1201 gcccgctcca gctcctcctg ggtggggccg tgctccgcca gctcccggag cagggcgcag
     1261 gcagcatcca gcgcctcccc ctccagctcg gggctgaggg cggtgtacac ccccagcagg
     1321 ccggtgtcgg cgtggtcggc tacataggag taagtggtgt agcacagccc ccgcttctcc
     1381 cgaagctcct gaaacagccg ggaggagacg ccgcccccca ggatggagtt gagcagcagc
     1441 agctggtaac ggcgggggtc cagatagctg aaggcgggga aggccaggat caggtggttc
     1501 tgctctgtgg gctttcgccg gacggtgaag gcggggcggt agcgggcccc cttgcagctg
     1561 gggacgtggc cgggctccag ctcagagagg atggcgcgca gctcctccac ggcctggggg
     1621 gtgaagctgc cggtgagggc ggtcaccaga ttgccggggc ggtagtgggc ctcctgatag
     1681 gcccgaagg
//
LOCUS       DBMDSJ010000403         1687 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15661_length_1687_cov_2.564943, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000403 DBMDSJ010000000
VERSION     DBMDSJ010000403.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15661_length_1687_cov_2.564943"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            175..1302
                     /locus_tag="ACZPAQ_08625"
     CDS             175..1302
                     /locus_tag="ACZPAQ_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR0904093.1"
                     /translation="MALATVVHPALAPSRRILAVSDIHGNRAFFRGLLEKAAFSSRDI
                     LVLVGDILEKGPDSLGLLRDILELSQDHTILPVCGNCDGLVLRFFEDDLWDQGFFRVY
                     LPAHPESILRQMAREAGFDRWDDLPRLRECLRRTFPWVWDWLRSLPTILENEHLVFVH
                     GGVPSLDRMEELDAWHCMKNDDYWGQGHSFAHTGKYVVVGHWPVTLYDPRIPSAAPIV
                     DRARHIISIDGGCVLKLDGQLNALIIPEESSPDFSWTAYDGLPTARALDPQAPSEDSL
                     NIRWGRNRVEVLERGPELSLCRHLETGRVLPILTSYLYDSPEGLRCEDSTDYLLPVVP
                     GDCLSIVARTGEMGTLVKKNGVTGWYRGRLNDETKRETKGT"
     gene            1307..>1687
                     /locus_tag="ACZPAQ_08630"
     CDS             1307..>1687
                     /locus_tag="ACZPAQ_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904094.1"
                     /translation="MLDFRPLQLEDLPRLRPYFAYSGSRLCDTTPGTVFIWRDMYRTE
                     YALWDNSLYFKVDYPGVGPTFTLPLGGGRLEQFRQIAEYCLQNKLPLRFVPVPKDELD
                     RMLHFFPGSTAQADREKVEHSVKLV"
BASE COUNT          298 a          571 c          469 g          349 t
ORIGIN      
        1 ccattccgtc cccctcctct ttccccttcc tgctaaaaac tccccgcaaa acgataggtt
       61 ttttctctcc cctttggctt gattctgtta cagcttctgt gttatgataa agtttagagc
      121 atcaggaccg ggcccattcc cgtccccaca cccgcccgga aaggagcacg cctcatggcc
      181 ctcgccactg ttgtccaccc cgccctggcc cccagcagac gcatcctggc ggtcagcgac
      241 atccacggca accgcgcttt tttccgcggt ctgctggaaa aagctgcttt ctcgtcccgt
      301 gatattctgg tgctggtagg ggacatccta gagaaagggc cggacagtct gggcctgctg
      361 cgggatatcc tggagttgtc ccaggaccac accattctcc ctgtctgcgg caactgtgat
      421 gggctggtcc tccgtttctt tgaagatgac ctgtgggacc aaggcttttt ccgggtctat
      481 cttcctgccc acccggagag tatcctgcgc cagatggcca gggaagccgg ctttgaccgc
      541 tgggacgacc tgccccgcct gcgggagtgc ctgcgccgga ccttcccctg ggtctgggac
      601 tggctgcgtt ccctgcccac catcctggag aacgaacacc tggtctttgt ccacggcggt
      661 gtcccctccc tggaccgcat ggaggagctg gacgcctggc actgcatgaa aaacgacgac
      721 tactggggcc agggccactc ctttgcccac accggcaagt atgtggtggt gggccactgg
      781 cccgtcaccc tgtatgaccc ccgcatcccc tccgctgccc ccatcgtcga ccgcgcacgg
      841 cacatcatct ccatcgacgg cggctgcgtc ctcaagctgg acggtcagct caacgccctt
      901 atcatcccgg aggaatcctc tccggacttc agctggaccg cctacgacgg gctgcccacg
      961 gcccgggccc tggacccaca ggcgccttcg gaggattccc tgaacatccg ctggggccgc
     1021 aaccgggtcg aggtgctgga gcggggaccg gagttatctc tgtgccgcca tctggagact
     1081 ggacgggtgc tccccatcct caccagctat ctttatgaca gcccggaggg gctccggtgc
     1141 gaggactcca ctgactacct tctgcccgtc gtgccggggg actgcctttc catcgtggcc
     1201 cgcaccggag agatgggcac cctggtaaag aaaaacggcg tcaccggctg gtaccggggc
     1261 cgcctgaatg acgagacaaa aagagaaaca aaaggaactt gatcgtatgc tggatttccg
     1321 acccctgcag ctggaggacc tgccccggct gcgcccctat tttgcttaca gcggcagccg
     1381 cctgtgtgac accacaccgg gcaccgtttt catctggcgg gacatgtacc gcaccgaata
     1441 cgctctgtgg gacaactctc tctattttaa ggtggactat cctggcgtag gtcccacctt
     1501 tacccttccc ttgggagggg gcaggctgga gcagttccgg cagatcgccg aatactgcct
     1561 gcagaacaag ctgcccctgc gttttgtccc cgtccccaag gacgagcttg accgaatgct
     1621 ccactttttc cctggaagca ccgcccaggc ggaccgggaa aaagtggagc attcggtcaa
     1681 gctcgtc
//
LOCUS       DBMDSJ010000404         1686 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15672_length_1686_cov_1.552050, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000404 DBMDSJ010000000
VERSION     DBMDSJ010000404.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15672_length_1686_cov_1.552050"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="ACZPAQ_08635"
     CDS             complement(<1..620)
                     /locus_tag="ACZPAQ_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR0904095.1"
                     /translation="MLYGCCVNLLPKTLDRIGLEYAGRLKRLGYDYIELPLNELAQLS
                     EQEFRDARTVLEELDLPCRSCNDFMPARFQITGPDVTSRAELTDYLRRALERAAWLGA
                     PYTVFGSPWSRSCPEHYSREAASGQIAEFLSLAGEEAAPYQVVIAIEHNNRGETNMLN
                     HFSDGVRMARTVDHPNVRVLCDYYHLRFEGDPPSVLLDGGSELLIHT"
     gene            749..>1686
                     /locus_tag="ACZPAQ_08640"
     CDS             749..>1686
                     /locus_tag="ACZPAQ_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR0904096.1"
                     /translation="MAVERDITWNIRHFQGALRCDRNFDVVYRVFRICGREACMFFVD
                     GFAKDEIMEKLLEFFYGIQDEDLLRDAHTFSKSCVPYCEVAISGEEDTIVGQILSGVW
                     AVFVDGMDQCVLIDQRTYPQRTTSEPDQDKSFRGSRDGFVETMVFNAALIRRRIRSPR
                     LCVEHLEVGETSRTDVAVCYFEGRADPELVNTVRERIAQARVDALTMNQESLAELLVR
                     KRWYNPFPKFKFTERPDAAAAQILEGDVVVLVDNSPAAIILPTTLFDVMEEANDYYFP
                     PITGSYLRLVRFFTTFLTTFLTPVWLLLIQEPERVPG"
BASE COUNT          302 a          480 c          575 g          329 t
ORIGIN      
        1 gtgtggatca acagctcgct ccctccgtcc agcaggacag agggggggtc cccctcgaac
       61 cgcaggtggt agtagtcgca caggacccgg acattggggt ggtccaccgt gcgggccatc
      121 cgtaccccgt cggagaagtg gttgagcatg ttggtctcgc cccggttgtt gtgctcgatg
      181 gcaatgacca cctgatatgg ggcggcctcc tcccccgcaa gggacaggaa ttcggcgatc
      241 tggccagagg ccgcctcccg ggagtagtgc tcggggcagc tgcgggacca gggactgccg
      301 aagaccgtat agggagcacc cagccaagct gcccgctcca gggcccgccg gaggtagtcg
      361 gtcagctccg ctctggaggt gacgtcgggg ccggtgatct gaaagcgggc gggcataaag
      421 tcgttgcagg agcggcaggg gaggtccagc tcctccagca ccgtccgggc atcccggaac
      481 tcctgctcgc tgagctgggc cagctcgttc aggggcagct caatgtagtc gtagcccagc
      541 cgcttcaggc gtccggcgta ttccaaaccg atccggtcca gtgttttggg gagcagattg
      601 acacagcagc cgtagagcat aggggggcct cctttgagtt cttttgttca tcctagcatg
      661 gggaaaaggg cgtgtcaagc atgcggcgga gggtatatcc cggcccgttc cgcggatact
      721 aatcaaaaaa tggatggagg agagggatat ggcggtggag cgggacatca cctggaacat
      781 caggcatttc caaggggctt tgcggtgcga ccggaatttc gatgtggtct accgggtgtt
      841 tcggatctgc gggcgggagg cctgcatgtt ttttgtagac ggctttgcca aggacgagat
      901 catggagaag ctgctggaat ttttctatgg gatacaggac gaggacctgc tccgggatgc
      961 ccatactttc tcaaagtcgt gtgtccccta ctgcgaggtg gccatcagcg gggaggagga
     1021 caccatcgtg gggcagatct tgtctggagt atgggcggtg ttcgtggatg gaatggacca
     1081 gtgcgtgctg atcgaccagc gcacctatcc ccagcggacc acctcggagc cggaccagga
     1141 caagtccttt cgggggagcc gggacggctt tgtggagact atggtcttca acgcggcgct
     1201 gatccgccgc cgcatccgct cgccccggct gtgcgtggag cacctggagg tgggggagac
     1261 ctccaggacc gacgtggcgg tgtgttattt tgagggccgg gcggacccgg agctggtcaa
     1321 cacggtgcgg gagcgcatcg cccaggccag ggtggacgct ttgaccatga atcaggagag
     1381 cctggcagag ctgctggtcc ggaagcggtg gtacaatccc ttccccaagt tcaagttcac
     1441 ggaacggccg gacgcggcgg cggcccagat cctggaaggg gatgtggtgg tgctggtgga
     1501 caattccccc gccgccatca tcctgcctac caccctcttc gacgtgatgg aggaggcgaa
     1561 tgactactac ttcccgccca tcaccggctc ctacctgcgg ctggtccggt tttttaccac
     1621 ctttctcact accttcctta ccccggtgtg gctcctgctg atccaggagc cggaacgggt
     1681 ccccgg
//
LOCUS       DBMDSJ010000405         1680 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15741_length_1680_cov_1.913870, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000405 DBMDSJ010000000
VERSION     DBMDSJ010000405.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15741_length_1680_cov_1.913870"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            625..1593
                     /locus_tag="ACZPAQ_08645"
     CDS             625..1593
                     /locus_tag="ACZPAQ_08645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0904097.1"
                     /translation="MTLKERGRRVRVTHPTPAQRAEAEERRLAPMMRALKAIHSASSP
                     ESMRPEDLKRQRAAQEVLGRLITPMIGMKWAPFELEGMNAAWVRPERSYDRNHAILYC
                     HGGGYTSGNLGYSRPLASKLSGVTGWDTLSFEYRLAPEHPFPAAVDDAMKAWDHLMRQ
                     GYGARDVTVAGDSAGGNLALVLCHRLKQEGRRLPARLILMSPWTDMTASGKSYTERAE
                     LDPTITLDYIRAVRGAYARDQELTSPMLSPLFGDFRGFPPTLIQAGSNELLLSDSVRL
                     RDRMQAAGVPCRLEVWTDMWHVFQMFPIKKAGQAMASVGRFLLEYT"
BASE COUNT          307 a          499 c          603 g          271 t
ORIGIN      
        1 ggaaccgggg catggttcgg tccactgccc gctggaggac ggcggggtcc accggctggg
       61 acaggacagc agaaaagcgg tagaccggcg agtacttctc ccgctggaga gcggagtaga
      121 ggacgccggc gttgtccagc cggtaccagt cagggggacc tttgggcgtc ttggacatgg
      181 cggtgcctcc tttcctttgt gatatcagtg tagcatctcc tgggggaaag tacaagggga
      241 ggggaaaaag tataaaattc aacgctccca ttcgctgcgg catgggcgtg ccttttctga
      301 ccttgcgcag ggagagctgc ttgaagaata tgccgtgggc gggaggcgga tcggtccttc
      361 ccctggcctc gtgggcctcc cgccacttcg gagagcctgg gctcttcggg ggcaggggag
      421 aggaatacgg cagttagatg aggtccatca taccgtatct ccacgcccgc cgcaaggcgt
      481 gcgcacctgc ccgcgcgatt ttgtcagaac gccgcagctt ttcccggcac cgcaaacaag
      541 gcggcgggga gaggccattg ccaaacgcgg ccggatgtgg taggcttgtc atgatccagg
      601 tcccagaaca ggaaggtgag aacgatgaca ctgaaagaga gaggaagacg ggtgcgggtg
      661 acccatccca cccccgccca gcgggcggag gcggaggagc gccgcctggc ccccatgatg
      721 agggccctga aggcaatcca ctcggccagc tcgcccgagt ccatgcgccc ggaggacctg
      781 aaacggcagc gggcggctca ggaggtgctg ggcaggctca tcacccccat gatcgggatg
      841 aagtgggccc cctttgagct ggaggggatg aacgcggcct gggtgcggcc tgagcggagc
      901 tatgaccgga accacgccat cctgtactgc cacggcgggg gctataccag cggaaatctg
      961 ggctactccc ggccgctggc ctctaagctg tccggcgtga cgggctggga caccctctcc
     1021 ttcgagtacc ggctggcccc ggagcacccc ttccctgcgg cggtggacga tgcgatgaag
     1081 gcgtgggacc atctgatgcg ccaaggctac ggggcccggg acgtgacggt ggcgggggac
     1141 tcggcgggag gaaacctggc gctggtgctg tgccaccggc tgaagcagga gggcaggcgg
     1201 ctgcctgccc ggctgatcct catgtcccca tggacggata tgacggccag tgggaagtcc
     1261 tatacagagc gggcggagct ggaccccacc atcaccctgg actatatccg ggcggtgcgt
     1321 ggggcctatg cccgggacca ggagctgacc tcccccatgc tgtccccgct gttcggggat
     1381 ttccggggct tcccgcccac actgatccag gcggggagca acgagctgct gctgtccgat
     1441 tccgtgcgcc tgcgggaccg gatgcaggcg gcgggggtcc catgccggct ggaggtctgg
     1501 accgacatgt ggcacgtgtt tcagatgttc cccatcaaaa aggcgggcca ggccatggcc
     1561 agcgtggggc gcttcctgct ggaatacaca tgaaaatggt ccctgtccgc aggagagcgg
     1621 gagggcacag cgggatcaag gagaaaagat cgagggccgc cccggtaggg gcggccctct
//
LOCUS       DBMDSJ010000406         1674 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15821_length_1674_cov_1.750795, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000406 DBMDSJ010000000
VERSION     DBMDSJ010000406.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_1674_cov_1.750795"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            357..1376
                     /gene="galE"
                     /locus_tag="ACZPAQ_08650"
     CDS             357..1376
                     /gene="galE"
                     /locus_tag="ACZPAQ_08650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR0904098.1"
                     /translation="MSILVSGGAGYIGSHTCVELIQAGYDIVVADNLVNSSREAIRRV
                     EQIVGRQVPFVEAELCDPEQVEALFQSHPDIRAVIHFAGLKAVGESVEKPLEYYTNNL
                     VNTLVLLNMMRKHGVKDFVFSSSATVYGNPGRVPFREDFPVGGTTNPYGTTKLFIEQI
                     LTDLCAADSSLNVALLRYFNPIGAHESGLIGEDPNGIPNNLVPYIAQVAVGKLEKLHV
                     FGGDYPTPDGTGVRDYIHVVDLARGHVSALKKLEERCGLFVCNLGTGKGYSVLEVLRA
                     YEKACGKTLPYVVDPRRPGDIAECYADPERAEREMGWKARYGIEEMCASSWKWQSMNP
                     DGYGR"
     gene            1391..>1674
                     /locus_tag="ACZPAQ_08655"
     CDS             1391..>1674
                     /locus_tag="ACZPAQ_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR0904099.1"
                     /translation="MEKPVLVVMAAGMGSRYGGLKQIDPVGANGQLIIDYSIYDARRA
                     GFETVVFVIKRELAEQFRAAIGDRLSRVIEVKYAYQELEDLPAGYGVPEGR"
BASE COUNT          364 a          425 c          560 g          325 t
ORIGIN      
        1 accgggcctt ttccagaaca tttcggccca ctatgcctgt acaacacttc tgacacagga
       61 ggaattggtt ataaaaaggg gaagatcagg ggaaaaatct ctgggatcac gcctaaaaag
      121 tttccctggt agacatttgg tagacacatg aaaacaggag gggcggcgag gccctgaaac
      181 tccaggcccc cgccgccctt ttttggtaga cttttggtag acatttttat ttttgagaga
      241 cgagaaagag acggaatcgg ttgcgtgatt tatgtttaga gattataatg gggccgtaac
      301 attgatcaga gaagctttcc aagggagagc ggatcatgac agaaaaggag cgatctatgt
      361 ccattttggt gagcggtggc gccggatata tcggcagcca cacctgtgtg gagctgatcc
      421 aggcgggata tgacatcgtg gtggcggaca atctggtgaa ttccagccgg gaagcgatcc
      481 ggcgggtgga gcagatcgtg ggaaggcaag tccccttcgt tgaggcggag ctgtgcgacc
      541 cagagcaggt ggaagcgctg ttccagagcc acccggatat ccgggcagtc atccatttcg
      601 cgggcctgaa agcggtgggg gagagtgtag agaagcccct ggagtactac accaacaatc
      661 tggtcaacac cctggtcctg ctcaatatga tgcggaagca cggcgtgaag gattttgtgt
      721 tctcctcctc tgctaccgtc tatgggaatc ccggccgggt acccttccgg gaggacttcc
      781 cggtgggggg gaccaccaac ccctatggga cgaccaagct tttcatcgag cagatcctca
      841 ccgacctgtg tgcggcggac agcagcctga acgtggccct gctgcggtat ttcaatccca
      901 tcggggccca cgagagcggg ctgatcgggg aggaccccaa cggcatcccc aacaatctgg
      961 tcccctatat cgcccaggtg gcagtaggga agctggagaa gctccatgtg ttcggcggcg
     1021 actatcccac gccggacggc accggggtgc gggactatat ccatgtggtg gacttggccc
     1081 ggggccatgt ctccgcgctg aagaagctgg aggagaggtg cggcctgttc gtctgcaatc
     1141 tggggaccgg gaagggttac tccgtgctgg aggtcctgcg cgcttatgag aaagcctgcg
     1201 gaaagaccct gccctatgta gtggatcccc ggaggccggg cgacattgcg gagtgctatg
     1261 cggacccgga gcgggcagag cgggagatgg ggtggaaggc gcgctacggc atcgaggaga
     1321 tgtgcgcctc ctcctggaag tggcagtcca tgaatccgga cggatacgga agataagagg
     1381 gggtggaagt atggaaaaac cggtgcttgt ggtcatggcg gccggaatgg ggagccgata
     1441 cggcggtctg aagcagattg atcccgtggg ggccaacggc cagctcatca tagattactc
     1501 catctacgac gccaggcgcg ccggctttga gacggtggta ttcgtcatca agcgggagct
     1561 tgcggagcaa ttccgggccg ccatcggcga ccggctctcc cgggtcattg aagtaaagta
     1621 cgcctatcag gagctggagg acctgcccgc cgggtacggg gtcccggagg ggcg
//
LOCUS       DBMDSJ010000407         1665 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15915_length_1665_cov_1.294757, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000407 DBMDSJ010000000
VERSION     DBMDSJ010000407.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15915_length_1665_cov_1.294757"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="ACZPAQ_08660"
     CDS             <1..408
                     /locus_tag="ACZPAQ_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS-related protein"
                     /protein_id="HIR0904100.1"
                     /translation="LFATHYHELTALEGEIPGVKNYNIAAKKRGNDLIFLRKIVRGGA
                     DQSYGIEVAKLAGVPDRVIRRARTILEELESGSGVLPAGGPALPADGEQVSLMDLGSQ
                     TVLKKLRMTDVNILSPIEALNLLAELKQDLQES"
     gene            429..947
                     /locus_tag="ACZPAQ_08665"
     CDS             429..947
                     /locus_tag="ACZPAQ_08665"
                     /inference="COORDINATES: protein motif:HMM:NF023580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HIR0904101.1"
                     /translation="MKEEVSGMGRPNSRAELCAWVLGLMGAFFLLFGGAVLLWDIPVG
                     GAGPDWAFLPIGAALLAAGGGCALACLARRRSQARLRREGTAVSGTVRSVRHHIFVTW
                     NTQSFVNVPGRNSPWSAVCGYTWGGRDYTVSTGLLWGEPQEGAEVLVWLDPDRPGRAF
                     VDPDSVRLDLMG"
     gene            1048..>1665
                     /gene="mutL"
                     /locus_tag="ACZPAQ_08670"
     CDS             1048..>1665
                     /gene="mutL"
                     /locus_tag="ACZPAQ_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR0904102.1"
                     /translation="MPEIQVLDPHVADLIAAGEVVERPASVAKELMENAIDAGARSVT
                     VEIAHGGLTFLRVTDDGCGIPAPQMKTAFLRHATSKLRTEYDLEAIGTLGFRGEALAA
                     ISAVSRIDVMSRTQDAPLGASLSLEGGVAGPVEQAGCPLGTTMVVRDLFYNTPARLKF
                     MKKDAAEGAAVFGVVQRVALSHPEVAVKFLRDGKQELATPGDGQLR"
BASE COUNT          254 a          532 c          627 g          252 t
ORIGIN      
        1 ctgttcgcca cccactacca cgagctcacc gccctggagg gggagatccc cggggtgaag
       61 aactacaaca tcgccgccaa gaagcggggg aacgacctga tcttcctgcg gaagatcgtc
      121 cgggggggcg cggaccagag ctacggcatc gaggtggcca agctggccgg ggtgcccgac
      181 cgggtcattc ggcgggcccg gaccatcctg gaggagctgg agtcggggag cggcgtcctc
      241 cccgcagggg gaccggccct tccggcggac ggggagcagg tctccctgat ggacctgggc
      301 agccagacgg tgctgaaaaa gctccggatg accgatgtga acatcctgtc ccccatcgag
      361 gccctcaacc tgctggcgga gctgaagcag gacctgcagg agagctgagg cggggcggag
      421 aggcccggat gaaagaggag gtgagcggca tggggcgtcc aaattctcgg gcggagctgt
      481 gcgcctgggt gctgggactg atgggggcgt ttttcctcct gttcggcggg gccgtcctgc
      541 tgtgggacat cccggtgggg ggcgcggggc ccgactgggc cttcctgccc ataggggccg
      601 ccctgctggc ggcggggggc gggtgcgccc tggcctgcct ggcccggcgg cggagccagg
      661 cgcggctgcg gcgggagggc acggcggtct ccggcaccgt ccgctcggtc cgccaccaca
      721 tcttcgtgac ctggaacacc cagtcctttg tcaatgtccc gggccggaac tcgccctgga
      781 gcgcggtgtg cgggtacacc tggggcggcc gggactatac cgtgtccacc ggcctgctgt
      841 ggggggagcc ccaggagggg gcggaggtcc tcgtttggct ggacccggac cggccggggc
      901 gggcctttgt ggacccggat tctgtccggc tggaccttat gggataaggg gcgggggagg
      961 cccggccccg tcccgggatc tgtattctga ctattatgac caaaaacggc ccgcaggagc
     1021 ggagctttga atttctggag gtgctctatg cctgagatcc aagtgttaga tccccacgtt
     1081 gccgacctga tcgccgccgg cgaggtggtg gagcggccgg ccagcgtggc caaggagctg
     1141 atggaaaacg ccatagacgc gggggcgaga tcggtgaccg tggagatcgc ccacggcggc
     1201 ctcactttcc tccgggtcac cgatgacggc tgcggcatcc cggcccccca gatgaagacc
     1261 gccttcctcc gccacgccac cagcaagctg cgcacggagt atgacctgga ggccatcggc
     1321 accctggggt tccgggggga ggcactggcc gccatttccg ccgtctcccg catcgacgtg
     1381 atgagccgca cccaggatgc cccgctgggg gcctccctgt ccctggaggg gggcgtggcc
     1441 gggccggtgg agcaggcggg ctgccccctg ggcaccacca tggtggtgcg ggacctgttc
     1501 tacaacaccc ccgcccggct gaagttcatg aaaaaggacg ccgccgaggg ggccgccgtc
     1561 ttcggcgtgg tccagcgggt ggccctgtcc cacccggagg tggcagtgaa attcctccgg
     1621 gacggaaagc aggagctggc cacccccggg gacggacagc tgcgc
//
LOCUS       DBMDSJ010000408         1663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15940_length_1663_cov_1.518566, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000408 DBMDSJ010000000
VERSION     DBMDSJ010000408.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15940_length_1663_cov_1.518566"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="ACZPAQ_08675"
     CDS             <1..488
                     /locus_tag="ACZPAQ_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR0904103.1"
                     /translation="LERLQVHLVLGYAGYYVAGWYLRHYTIGRVPEFLLYLLGILGLV
                     LTLGGDALLGGGRELWYSDTAPGVALTAAALCALFRYVLGISEERSRRRAVHALGSYV
                     FGAYLIHQLWMLLFRWLGLTVTALEPVFFVPLLALVLLALSLPMAWLLRLIPGAGKWL
                     T"
     gene            complement(565..1368)
                     /locus_tag="ACZPAQ_08680"
     CDS             complement(565..1368)
                     /locus_tag="ACZPAQ_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025056.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR0904104.1"
                     /translation="MDRDYIWEGEYGRRSHLIPVFQQLLREQDASSLQMLSLFVNNNP
                     YNLEFFIAVSYDRPKEEHLDRMFRLLEDAGLRQRKDLTALNIMRAMADRRFNSASLLD
                     EDSVPTIMGHLFFFPEIRVLEEKGYRMTPFGREQMVFLSHSSLDKPEIEEIIPLLNGM
                     DLPVWFDKYSIALGDSITDKVQEGIQQSSIVLFWITEHFLDSRWCKFEMRAFIKKLIE
                     EDVRVISVLDRGIQISRLPLFLQDIKCLMREERTIYQVAQQIASALRPS"
     gene            complement(1372..>1663)
                     /locus_tag="ACZPAQ_08685"
     CDS             complement(1372..>1663)
                     /locus_tag="ACZPAQ_08685"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR0904105.1"
                     /translation="TPGFAPEGGEVYPSLDALLAAAPADAFVIGGESVYRALLDRCGT
                     AYVTKILAQYPADRYFPDLDADPRWESVEESELLEENGVRFRYVTYRRRQEA"
BASE COUNT          298 a          482 c          551 g          332 t
ORIGIN      
        1 tgctggagag gctccaggtc catctggtgc tggggtatgc gggctattat gtggcggggt
       61 ggtatctgcg ccactatacc atcgggcggg tgccggagtt tctgctctac ctgctgggaa
      121 tcctggggct ggtcctgact ctgggtggcg acgcgctgct gggcggcggg cgggagctgt
      181 ggtacagcga caccgccccc ggggtggccc tgacggcggc ggcgctgtgc gctctgttcc
      241 gctatgtgct ggggatcagt gaggagcggt cccgccgccg ggcggtccat gccctgggca
      301 gctatgtctt cggcgcctat ctgatccacc agctgtggat gctgcttttc cggtggctgg
      361 ggctgacggt caccgccttg gagccggtgt tctttgttcc cctgctggcc ctggtgctgc
      421 tggccctgtc cctgcccatg gcctggctcc tgcggctgat cccaggggcc ggaaaatggc
      481 tgacctgaga cgacaaaagg ggagccggcg catggcgcgc cggccccctt ttcaaatgga
      541 gtggttctaa atttttcaag tcactcagct gggccggaga gcggaggcga tctgctgggc
      601 gacctgataa atggtccgtt cttcccgcat gagacacttg atgtcctgga gaaagagcgg
      661 caggcgggag atctggatgc cccggtccag aacggagatg acccggacgt cctcttcgat
      721 cagcttcttg atgaaggccc gcatctcaaa tttacaccag cgggaatcca gaaagtgctc
      781 tgtgatccag aagagcacga tgctggactg ctggatgccc tcctggacct tgtcggtgat
      841 ggaatcgcca agggcaatgc tgtatttgtc gaaccagacg ggcagatcca tcccattgag
      901 cagcgggatg atctcttcga tctccggctt atccagagag gagtggctga gaaagaccat
      961 ctgttcccgg ccaaagggag tcatacggta gcctttctcc tccaggaccc ggatctccgg
     1021 gaagaaaaag aggtgcccca taatggtggg aacactgtcc tcatccagca gagaggcaga
     1081 gttgaagcgc cggtccgcca tggcccgcat gatattcagc gcggtcaggt ccttccgctg
     1141 ccgcagtcca gcgtcctcca gaagacggaa catccggtcc agatgctcct ccttggggcg
     1201 gtcgtagctc actgcgatga aaaattccag attgtaaggg ttgttgttga cgaacaggga
     1261 gagcatctgg agcgaggagg cgtcctgttc ccgcagcagc tgctggaaca ccgggatcag
     1321 gtgggaacgg cggccgtact ccccttccca gatatagtca cggtccatgg gtcatgcctc
     1381 ctgcctgcgc cggtaggtca cataccggaa gcgtactccg ttctcctcca ggagttctga
     1441 ctcctccacc gactcccagc gagggtcggc atccaggtcc gggaagtacc ggtctgcggg
     1501 gtactgggcc aggatcttag tgacataggc ggtgccgcac cggtccaaaa gggcgcggta
     1561 gacggactcc ccgccgatga caaaggcatc cgccggagcg gcggcgagga gggcgtccag
     1621 ggagggatag acctcaccgc cctccggggc gaagcccggc gtg
//
LOCUS       DBMDSJ010000409         1662 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15960_length_1662_cov_1.030750, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000409 DBMDSJ010000000
VERSION     DBMDSJ010000409.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15960_length_1662_cov_1.030750"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="ACZPAQ_08690"
     CDS             complement(<1..394)
                     /locus_tag="ACZPAQ_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904106.1"
                     /translation="MSDKSERIFQSLSDISDQKVDEAARRPAGKRPAPRWRRWTALAA
                     CLCLVAGAAVWYLPRMGGSSSNNGLGGSGADGVTNFMSYAGPVFPLTLAEENGAVTAQ
                     REITLDFQPWVPRWISNEEMAAEEAAWGE"
     gene            complement(391..948)
                     /locus_tag="ACZPAQ_08695"
     CDS             complement(391..948)
                     /locus_tag="ACZPAQ_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0904107.1"
                     /translation="MEDTDIIQLYWARDEGAIAATDEKYGTLCRSLSHNILASREDAE
                     ECVNDTWHRAWNTMPPQRPASLRAYLVRLVRNLSIDRWRARKSQKRGEGLEALVLELE
                     DCVPAAPSAEEETEARELSRTIDRWLDGLPREDRVLFVRRYWYGQRLDELSGQLGWSP
                     NRVSQRLLRLRTGLKRYLEREGVVL"
     gene            1186..1479
                     /locus_tag="ACZPAQ_08700"
     CDS             1186..1479
                     /locus_tag="ACZPAQ_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR0904108.1"
                     /translation="MSKFLDKEASDTLYRAILTLKDEDECRRFLQDLCTVSELKAMEQ
                     RMEVAMLLDEGLIYSDILEKTGASSATISRVNRCLHYGAEGYQRVIPRLKERR"
     gene            1479..>1662
                     /locus_tag="ACZPAQ_08705"
     CDS             1479..>1662
                     /locus_tag="ACZPAQ_08705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0904109.1"
                     /translation="MNSYRFLAGCYDQLTYDVRYPAWADYIEKHFRRQPLPGRTVLDL
                     ACGTGSLTRELALRGYE"
BASE COUNT          309 a          548 c          475 g          330 t
ORIGIN      
        1 cctcccccca ggccgcttct tcggcggcca tctcctcatt agagatccac cggggcaccc
       61 agggttgaaa gtccagagtg atctcccgct gggcggtgac agccccgttt tcctccgcca
      121 aggtcagggg aaacaccggg cctgcatagg acatgaaatt ggtcacccca tccgctccgc
      181 tcccgcccag gccgttgttg gaggagcttc cccccattcg gggcagatac cacactgccg
      241 cccccgccac caggcacaga caggcggcca gggcggtcca ccgcctccaa cggggggcgg
      301 gccgctttcc ggccggtctc cgggccgcct catctacctt ctggtctgaa atgtcgctca
      361 gagattgaaa gatcctctca ctcttgtcac tcacagcact accccctctc gctccagata
      421 ccgcttcaga cccgtccgca ggcggagcag ccgctggctc acccggtttg gggaccagcc
      481 cagctgaccg gacagctcat ccagccgctg tccgtaccag taccggcgga caaacagaac
      541 tctgtcctct ctcggcagac cgtccagcca gcggtcaatg gtccgggaga gctcccgcgc
      601 ctctgtctcc tcctcggcgc tgggagcggc tggcacacag tcctccagtt ccagtaccag
      661 cgcctccagc ccttctcccc gcttctggct cttccgggcc cgccagcgat cgatggacaa
      721 attgcggacc aatcggacca gataggcccg cagggaggca ggccgctggg gcggcatggt
      781 attccaggcc cggtgccagg tgtcgttgac acactcctcc gcgtcctccc ggctggccag
      841 gatattatgg gacagagagc ggcacagcgt gccgtatttt tcgtcggtgg ccgcaatggc
      901 cccctcgtcc cgggcccaat acagctggat gatgtctgtg tcctccacgg ccgcacctcc
      961 ttccaaaatc ctattcatcc ttctagacgt aaaagaacgc gcgatatgac atcggattga
     1021 ttttttattt tatcatgggc cggtgaaaaa agctacgagg ggcggacctc gggtgccggt
     1081 cctctggctg tatttttttg tttactttca catggtagcc tgttaaaata tagaagtgtg
     1141 gagatatccc ccacccttgg aatcatcggg agaaaggagc ctgttatgtc aaaatttttg
     1201 gataaagagg ccagcgatac cctgtatcgc gccattttga ccctgaagga cgaggacgag
     1261 tgccgccgct ttctccagga cctgtgtacc gtgtccgagc tgaaggccat ggagcagcgg
     1321 atggaagtgg ccatgctgct ggatgagggc ctgatttaca gcgacattct ggaaaagact
     1381 ggggcctcca gcgccaccat cagccgggtg aaccggtgcc tccactacgg ggcggagggc
     1441 tatcagcggg tcatcccccg cctgaaggag cggcggtgat gaacagctac cgctttctgg
     1501 ccggctgcta tgaccagctg acctatgatg tccgctatcc cgcctgggcc gactatatcg
     1561 aaaagcactt ccgccgccag cccctgccag gcaggacggt gctggacctg gcctgcggca
     1621 cgggctccct cacccgggag ctggccttgc ggggctatga ga
//
LOCUS       DBMDSJ010000410         1661 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_15967_length_1661_cov_1.748718, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000410 DBMDSJ010000000
VERSION     DBMDSJ010000410.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_1661_cov_1.748718"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="ACZPAQ_08710"
     CDS             complement(<1..486)
                     /locus_tag="ACZPAQ_08710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR0904110.1"
                     /translation="MKITVLGSGGWGTALALLLLENGHEVTLWSYTEEESRVLRETRE
                     NPMLKGVPLPETMGLTTDMGAVKGCGAVVMATPSFAVRATAAKLKQLADPGTIVISVS
                     KGIEKDTSLRLSQVIEEELQGHCPVVVLSGPSHAEEVGRHVPTGVVAASEDLELARQV
                     QD"
     gene            complement(584..1264)
                     /locus_tag="ACZPAQ_08715"
     CDS             complement(584..1264)
                     /locus_tag="ACZPAQ_08715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR0904111.1"
                     /translation="MTWGDISPWIFYDMGLPLLLVAAAAYLLGCSNGAVIVSKYILRD
                     DVRTHGSGNAGLTNFYRTFGGPLTLVVILCDVLKAVAAVWAGILMARYWDGGFWSQEA
                     IVLVFAKYWAGLFCLLGHMFPCMFHFKGGKGILSGGTIAIMIDWRIALVVWGGFLILA
                     ILTRYVSLGSVWAGASFPVATWFVYQDWIITLLGLLLGGLVVYMHRGNIHRLLTGTEN
                     KFSLHKKQ"
     gene            complement(1277..>1661)
                     /locus_tag="ACZPAQ_08720"
     CDS             complement(1277..>1661)
                     /locus_tag="ACZPAQ_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR0904112.1"
                     /translation="MRDLSYMTYAPIVFISALTGQRVDRLFELINYVNDQAATRISTG
                     MLNSVLADATARVQPPTDKGRRLKIYYMTQVGIRPPHFVCFCNDARLFHFSYQRYLEN
                     QIRATFGLEGTPVRLTIRQKGEKEE"
BASE COUNT          311 a          538 c          529 g          283 t
ORIGIN      
        1 gtcctgcacc tgccgggcca gctccaggtc ctcgctggcc gccaccacgc cggttgggac
       61 atggcggccc acctcctcgg cgtgggaggg gccggagagc accaccacgg ggcagtggcc
      121 ctggagctcc tcctcaatga cctggctgag gcggagggag gtgtccttct cgatgccctt
      181 ggacacggag atcacgatgg tccccgggtc ggccagctgc ttcagcttgg cggcagtggc
      241 gcggaccgca aaggacgggg tggccatcac cacggcgccg cagcccttca ccgcccccat
      301 atcggtggtc aggcccattg tctcgggcag cggcaccccc ttcagcatgg ggttctcccg
      361 ggtctcccgc agcacccggg actcctcctc ggtgtaggac cacagggtca cctcatgtcc
      421 gttttccagc agcagcagcg ccagggccgt cccccagcct ccgctgccaa ggactgtgat
      481 tttcatggta atacctccca agatatgttg taggggcggc ctgtggccgc ccgttgttca
      541 attttcttgg ctgttcgggc ggccgcaggc cgcccctacg gggtcattgc ttcttgtgca
      601 gagaaaactt gttctctgtc ccggtcagca gacggtggat gttgccccgg tgcatgtaga
      661 ccaccaggcc gcccaagagc aggcccagca gggtgatgat ccagtcctga tagacgaacc
      721 aggtggccac cggaaagctg gctccggccc acaccgatcc cagggagacg tatcgggtga
      781 gaatggcaag gatcaggaat cccccccaca ccaccagggc aatgcgccag tcgatcatga
      841 tggcgatggt gccgccggac aggatgccct tgcccccctt gaagtggaac atgcagggga
      901 acatgtggcc cagcaggcaa aacaggcccg cccagtactt ggcaaagacc agaacgatgg
      961 cctcttggct ccagaagccg ccgtcccaat atcgggccat caggatgccc gcccacactg
     1021 cggccaccgc cttgaggaca tcgcacaaaa taaccaccag ggtcagcggc ccgccgaagg
     1081 tgcggtagaa gttggtcagc ccggcgttgc cgctaccgtg ggtgcgcaca tcgtcccgca
     1141 ggatgtactt ggagacgatg accgccccgt tggagcagcc cagcaggtag gccgctgccg
     1201 ccaccagaag caagggcaga cccatgtcat aaaagatcca gggggagatg tccccccaag
     1261 tcatgcccag cattgctcac tcctccttct cgcccttctg ccggatggtc agccgcaccg
     1321 gcgtgccctc caggccgaag gtggctcgga tctggttctc cagataccgc tgataggaga
     1381 agtggaacag ccgggcgtcg ttgcagaagc acacaaaatg aggggggcgg atgcccacct
     1441 gggtcatgta gtagatcttc aggcggcggc ccttgtccgt ggggggctgc acccgggcgg
     1501 tggcatcggc cagcaccgag ttgagcatcc cggtggagat gcgggtggct gcctggtcat
     1561 tcacatagtt gatcagctcg aagagccggt ccacccgctg gccggtgaga gcggagatga
     1621 acacgatggg ggcgtaggtc atatagctca ggtcccgcat c
//
LOCUS       DBMDSJ010000411         1655 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16027_length_1655_cov_1.822394, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000411 DBMDSJ010000000
VERSION     DBMDSJ010000411.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16027_length_1655_cov_1.822394"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="ACZPAQ_08725"
     CDS             complement(<1..298)
                     /locus_tag="ACZPAQ_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015572822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR0904113.1"
                     /translation="MDIQGLQKLTLLDWPGRVACTIFLGGCDFRCPFCHNRDLVTGPL
                     PAAMDSGELLAFLAKRKGLLDGVCVTGGEPLLRPGLEGLLEEIKALGFPIKLDTN"
     gene            complement(346..>1655)
                     /locus_tag="ACZPAQ_08730"
     CDS             complement(346..>1655)
                     /locus_tag="ACZPAQ_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0904114.1"
                     /translation="TRGSVPLCLIRFSFPLLLANLLQSFYSMADLLVVGRFVGETGLA
                     AVSSASTLCFLLNAVCMGFSAGGTVLTARARGAGDRLEEEEAAAALLLLALAGSALLT
                     AAALLCYRPLFTLMALPSQAMGDACGYMAILCWGTPFVFGYNAVCAILKGRGDSRGPL
                     RFVAAAAVVNLLLDLILAGPCGLGTAGTAWATVAAQGVSFLLSLAHLHRCVPLSLRRL
                     PLRPAALLSLLRLGLPSAVQMAVVNLSYLILTGLLNPFGVSVAAAAGIGLKVSTFAGM
                     PCWALGYGVTAMVGQNLGAGDPDRVRQTVRSGLLLSLGATALLTALVQLAAAPIIALF
                     EPENAAVTAEGVRYLHICCSVNGLIYAAMYVFDSFAIGSGASWVAMCNAMLDAALLRL
                     PLCWLLAFPLALGPAGIYLGQAFSPILPALAGLCYFRLRCRRS"
BASE COUNT          335 a          511 c          626 g          183 t
ORIGIN      
        1 cgttggtgtc cagcttgatg gggaagccca gggccttgat ctcctccaga agtccttcca
       61 gcccggggcg gagcaggggc tctccgccgg tgacgcagac cccgtccagc agccccttcc
      121 gcttggcgag gaaagccagc agctccccgc tgtccatggc ggcggggagg gggccggtca
      181 ccaggtcccg gttgtgacag aaggggcagc ggaagtcaca gccccccagg aaaatcgtgc
      241 aggccacccg gcccggccag tccagcagcg tcaatttttg cagtccctga atgtccatgg
      301 tcagcgctcc ttttagctct attgtagcgg cgggaggggg agccgtcaag accggcggca
      361 ccggagacgg aaatagcaca gccccgccag ggcgggcagg atgggggaga acgcctggcc
      421 caagtaaatg ccggcggggc ccagggcgag ggggaaggcc agcagccagc acaggggcag
      481 acggagcagg gccgcgtcca gcatggcatt gcacatagcc acccaggaag ccccagagcc
      541 gatggcgaag gagtcgaaga catacatggc agcgtagatc agtccgttga cggagcagca
      601 gatgtggaga tagcgcaccc cctctgccgt cacggccgcg ttctccggtt cgaagagggc
      661 gatgatgggg gcggcggcca gctggacgag ggcggtcaga agggcagtgg cccccagact
      721 gaggagaagg ccgctcctga cggtctgacg cacccggtcc ggatcgccgg cacccaggtt
      781 ctgccccacc atggcggtca ccccatagcc cagcgcccag cagggcatcc cggcgaaggt
      841 gctgaccttc aggccgatgc cggccgccgc cgccacggag acgccgaaag ggttgagcag
      901 gccggtgagg atcaggtagg agaggttgac caccgccatc tggacggcag aggggaggcc
      961 cagccggaga agggagagga gggcggcggg gcggaggggg agacgccgga gggagagggg
     1021 gacacagcgg tgcaggtggg ccagagagag cagaaaggag acgccttggg ccgccacggt
     1081 ggcccaggcg gtcccggcgg ttcccaggcc gcagggacct gccaggatca gatccaggag
     1141 gagattgacc acagcggcgg cggccacgaa ccggagggga ccgcgggagt cgccccggcc
     1201 cttcaggatg gcgcagacgg cgttgtatcc aaagacaaag ggggtgcccc agcagaggat
     1261 ggccatatag ccgcaggcgt cccccatagc ctgggaaggc agggccatca gggtgaacag
     1321 cgggcggtag cagagcaggg cggccgccgt cagcagggcg gagcctgcca gcgccagcag
     1381 gagcagtgcg gcggcggcct cctcctcttc caggcggtcc ccggcacccc gggcccgggc
     1441 ggtgaggacc gtcccaccgg cggaaaagcc catgcacacc gcgttgagga ggaagcacag
     1501 ggtggaggcg ctgctcacag cggccaggcc ggtctcaccc acgaagcggc ccaccaccag
     1561 cagatccgcc atgctgtaaa aggactggag cagattggcc agcaggagag ggaaggagaa
     1621 tcggatcagg cagaggggga cgctcccccg ggtca
//
LOCUS       DBMDSJ010000412         1654 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16041_length_1654_cov_1.889247, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000412 DBMDSJ010000000
VERSION     DBMDSJ010000412.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16041_length_1654_cov_1.889247"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="ACZPAQ_08735"
     CDS             complement(<1..324)
                     /locus_tag="ACZPAQ_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0904115.1"
                     /translation="MQIQIQDLSMTYPSGKQALQRLDLDLTSPSLVGLLGPNGAGKST
                     LMKLLVAALLPTQGSILVDGRPLDRSQRALKARLGYLPQSFGLYDELTVYQFLDYMAA
                     LKGLRN"
     gene            complement(472..>1654)
                     /locus_tag="ACZPAQ_08740"
     CDS             complement(472..>1654)
                     /locus_tag="ACZPAQ_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR0904116.1"
                     /translation="PLSQAQDTPSPDHMLGLTAEQRMEDYKYLWSVLADSYPCWGILD
                     REGVDVEGIYTSYRDMVAESDSDQTFYQAVYSTLFLLGNNGHLSIVEPADYFSLLPSA
                     RLYRRSGQTAWADILERKETREKYRSLLALDRAINSSSEEEEPALETPAPAAGGVIPL
                     LLPESGVGYVKIDSFPAEYEADRAILSDFYRQCGDCTDLIFDLTDNSGGSEAYWEELL
                     VAPHIDEPLSSEHLALVRHSRNNAPYLDQVFPAECLLPLNQLPSLPHLEPGDLDLATH
                     FVRVCHRVEPADQPSPFRGRIWVLVDESVYSASESFALFCQQTGFATLVGSATSGDGI
                     GTDPVFLQLPNSGILVQYTPVFGLNPDGSSNEELGTLPDLPSPPGEPPLITALRAISQ
                     A"
BASE COUNT          323 a          461 c          569 g          301 t
ORIGIN      
        1 gttcctcagc cccttcaggg cagccatgta gtccaggaac tgatagacag tgagctcgtc
       61 atacaggccg aaggactggg ggagatagcc cagacgggct ttgagcgccc gctgggatcg
      121 gtccagcggg cgcccgtcca ccaggatgga gccctgggtg ggcaggaggg cggccaccag
      181 cagcttcatc agggtggact tgcccgcccc gttggggccc agaagcccca ccaggctggg
      241 gctggtcagg tccaggtcca gtcgctggag ggcctgcttc ccgctgggat aggtcataga
      301 gagatcttgg atttggattt gcatgggtca acatcctttc gtcttgggtt ccatgtcagg
      361 ataccggtgc aatgtggaga gcgattgaag aaaagatgtg ttttgtatga agatgacgga
      421 gaggccaggg caatcatgtc cccgcccggg aggcggacgg ggacagatgg cttaggcctg
      481 agagatggcc cggagagcag tgataagagg aggctcgcct gggggagagg ggaggtctgg
      541 gagggtgccc agctcctcgt tgctgctccc gtcgggattg aggccgaata caggggtgta
      601 ctggaccaag ataccgctgt tgggcagctg gagaaagacc gggtctgtcc cgatgccgtc
      661 tccgctggtg gcactgccca ccagcgtggc aaaaccggtc tgttggcaga agagggcgaa
      721 ggattcggag gcggagtaga cggactcgtc taccaggacc cagatgcggc cccggaaggg
      781 gctgggctga tcagccggct ccaccctgtg gcagactcgg acaaagtggg tggctagatc
      841 caggtccccc ggctcaagat gaggcagact ggggagctgg ttcaggggga ggagacactc
      901 ggcgggaaag acctgatcca aataaggggc gttgttgcgg ctgtggcgga ccagggccag
      961 gtgctcgctg gacaggggct catcaatgtg gggggcgacc aacagctcct cccagtaagc
     1021 ctcgctcccg ccgctgttgt ccgtcagatc aaagatcagg tcggtgcagt ccccacactg
     1081 ccggtagaag tcagacagga tggcacggtc cgcctcgtac tcggcgggaa aactgtcgat
     1141 tttcacatag cccaccccac tctccggcaa caggaggggg atcacgccgc cagcagcggg
     1201 ggccggcgtc tccagggccg gctcctcttc ctcacttgag gagttaatgg cgcggtccag
     1261 ggccaggagg gagcggtact tttcgcgggt ctctttccgc tcgagaatgt cggcccaggc
     1321 cgtctgcccg ctccgccggt agagcctggc ggatgggagg agactgaagt agtcggcggg
     1381 ctccacaatg gacagatgcc cgttgttccc cagcaggaac agggtgctgt aaaccgcttg
     1441 ataaaaggtc tgatcgctgt cgctttcggc caccatgtcc cggtagctgg tgtagatccc
     1501 ttccacatcc accccttccc gatctaaaat cccccagcag ggatagctgt cggccagaac
     1561 agaccagaga tacttgtagt cctccatccg ctgctctgcg gtcagaccca gcatgtgatc
     1621 tggagaaggg gtgtcctggg cctgactgag cggc
//
LOCUS       DBMDSJ010000413         1644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16155_length_1644_cov_1.987038, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000413 DBMDSJ010000000
VERSION     DBMDSJ010000413.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16155_length_1644_cov_1.987038"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1644)
                     /locus_tag="ACZPAQ_08745"
     CDS             complement(<1..>1644)
                     /locus_tag="ACZPAQ_08745"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR0904117.1"
                     /translation="WGLAEPAPGPHQTTVLFTHDLHSHFLPLERADGGESGGYARLAS
                     ALEQQRALHPDALTLDGGDFSIGSLVQTLYTTRAAELRTMGALGYDATTAGNHEFDHT
                     GLGFAQMLTAAYTSGDPLPALLLANYAPSAGGPDQLDIQRAMSAYGVTDTLLLERGGV
                     TYGIFGLMGTDSDDCAPTSGFTLEDPVQAAQRCVDSLEEQGAQFIIALSHSGTDERER
                     KSEDQQLAREVEGIDLILSGHTHTTLTQPIVEGDTYIVSAGPYCENLGSITLEWSEDG
                     SKTLVDYQLIPIDQTLPEDQEIASLVEEWKAQVGVDYLARYHLTYDQVLARADFSLTR
                     PESGVQQGNPLGELVADAFLWAARELAAEDPGVPTVAVTADGVLRADLPAGDITVSQA
                     FDVLSMGVGEDGTSGFPLVTCYLTGKELRAAAEVDASVTPIMPAAQLYMSGMVYTFNP
                     HRMFFNRVTSCWLEEPSFATGLLEREMSRIEDDQLYRVVTGMYSAQMLGTVKEKSLGL
                     LSLEPKDENGVPITDFATRVLRDGNGSEIKEWYALAAPL"
BASE COUNT          279 a          565 c          533 g          267 t
ORIGIN      
        1 gaaggggtgc cgccagggca taccactcct tgatctcgct gccgttcccg tcccggagca
       61 cccgggtggc aaaatcggtg atgggcactc cgttctcgtc cttgggctcc agagacagca
      121 gccccaggga cttctccttc accgtgccca gcatctgggc ggagtacatc ccggtcacca
      181 cccggtagag ctggtcgtcc tcgatccggg acatctcacg ctccaacagg ccggtggcga
      241 aggagggctc ctccagccag caggaggtca cccggttgaa aaacatccgg tgggggttga
      301 aggtgtacac cattcccgac atgtacagct gggccgccgg catgataggg gtcaccgagg
      361 cgtccacctc tgccgccgcc cgcagctcct tcccggtgag gtagcaggtc accagtggaa
      421 aaccagaggt cccatcctcc cccactccca tggagagcac atcaaaggcc tgggagacgg
      481 tgatatcccc cgcaggcaga tcggcccgca gcacgccgtc ggcagtgact gccaccgtgg
      541 gcacccctgg gtcctccgct gccagctccc gcgccgccca gaggaaggcg tccgccacca
      601 gctcccccag gggatttccc tgctggacgc ccgactccgg ccgcgtcagg gagaagtccg
      661 cccgggccag cacctggtca taggtcaggt ggtagcgggc cagataatcc acccccacct
      721 gggccttcca ctcctccacc agggaggcga tctcctggtc ctccggcagg gtctggtcga
      781 tagggatcag ctggtagtcc accagggtct tgcttccgtc ctccgaccac tccagagtga
      841 tgctccccag attctcgcag tagggcccgg cggagacgat ataggtatcc ccctccacaa
      901 tgggctgggt gagggtggtg tgggtatggc cagagaggat caggtcgatc ccctccacct
      961 ccctggccag ctgctggtcc tccgacttcc gctcccgctc gtcggtcccg ctgtgggaca
     1021 gcgcgatgat aaactgcgca ccctgctcct ccaggctgtc cacgcagcgc tgggccgcct
     1081 gcaccgggtc ctccagcgtg aagcccgaag tgggggcgca gtcgtccgag tcggtgccca
     1141 tcaggccgaa aatgccgtag gtgaccccgc cccgctccag cagtagcgtg tcggtcacgc
     1201 cgtaggccga catggcccgc tggatgtcca gctggtcagg accaccggcc gatggggcgt
     1261 agttggccag gagcagggcc ggaagcggat ctccgctggt gtaggccgcc gtgagcatct
     1321 gggcaaagcc caggccagtg tggtcaaact catggttgcc cgcagtcgtg gcgtcataac
     1381 ccagagcgcc catggtccgc agctccgccg cccgggtggt gtagagggtc tggaccagag
     1441 agccgatgga gaagtctccc ccgtccaggg taagagcatc cgggtggagg gcccgctgct
     1501 gctccagtgc ggaggccagc cgggcatagc ctccggactc cccgccgtcc gccctctcca
     1561 agggcaggaa gtgggagtgg agatcatggg taaagagcac cgtggtctgg tggggcccag
     1621 gggccggctc cgccagaccc cagg
//
LOCUS       DBMDSJ010000414         1629 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16340_length_1629_cov_1.528141, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000414 DBMDSJ010000000
VERSION     DBMDSJ010000414.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16340_length_1629_cov_1.528141"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="ACZPAQ_08750"
     CDS             <1..791
                     /locus_tag="ACZPAQ_08750"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR0904118.1"
                     /translation="ADRSVALGDPDFVQVDVERLTSKAHAAERYALSSPETAGEYAPA
                     EGIEAKEYPGNTSHFAVMDRFGNAVSQTQTVRDWFGCGIVVDGCGFVLNNAMSDFSAK
                     PGALTSQGLTYGSANSIQGGKTPLSSMAPSMVFKDGRPYLAIGAAGGPRIITGTLQGI
                     VNAVDFGMLPEQLVRQPYLNCLTREQGLELEFGISEDTIRLLEQKGHRPVRVPVDQAM
                     STMLNSVMYVDGEYHAAGTQRVDGCGGALLSDGHMVLDGISQED"
     gene            complement(926..>1629)
                     /locus_tag="ACZPAQ_08755"
     CDS             complement(926..>1629)
                     /locus_tag="ACZPAQ_08755"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR0904119.1"
                     /translation="GMPMEQALVRHIYPDGQEIIEVGGAGILTYPAVGINSHGLAVTT
                     TGFWTEKDPTRIDRVEEADIFLNVRVLLSSCRTAAQAVEFCRTAPRMNGLNVIAADPT
                     EAYVIEMTSDEIYVEKDEGRGVLFRTNHVVSDQLSHFNPPEENYPSTHKRYDRIAQMV
                     EERYGSLRFQDLYRIMSDHTNEPNCICRHPHEGVPATTVSTTLCVVEDREVWTTLQNP
                     CLALPHVQIGEPSAQ"
BASE COUNT          313 a          459 c          566 g          291 t
ORIGIN      
        1 ttgcggaccg aagcgtggct ctgggcgatc ccgacttcgt ccaggtggac gtggagcggc
       61 tgaccagcaa ggcccacgcg gcggagcggt atgccctctc ctcaccggag acggcggggg
      121 aatacgctcc ggcggagggg atcgaggcca aggagtaccc aggaaacacc tctcactttg
      181 cagtgatgga ccgctttggg aacgcggtct cccagaccca gactgtacgg gactggttcg
      241 gctgcgggat cgtggtggac ggctgcggct ttgtgctcaa caacgccatg tccgacttca
      301 gcgccaagcc gggcgcactc accagccagg gactgaccta tgggagcgcc aacagcatcc
      361 agggggggaa gacgcctctg tcctccatgg cccccagcat ggtgttcaag gacggacggc
      421 cctatctggc catcggcgcg gcgggcggtc cccggatcat caccggaacc ctccagggga
      481 tcgtcaatgc ggtggacttt gggatgctgc cggagcagct ggtccgccag ccctacctca
      541 actgcctgac ccgggagcag gggctggagc tggagttcgg gatctcagag gacaccatcc
      601 ggcttctgga acagaagggg caccggccgg tgcgggtccc ggtggaccag gccatgagca
      661 ccatgctcaa cagcgtcatg tatgtggacg gggagtacca cgctgccggc acccagcggg
      721 tggacggctg cggcggagcc cttctctctg acgggcatat ggtgctggac gggatcagtc
      781 aggaggattg agcggcctgc ctgaagcatc aaaaagcgaa aagagaaaaa tggtcagcag
      841 aatttctgct gaccattttt tgatattggg aaccaataaa gagaagggac ggtgcgtacc
      901 ggggccagaa cctggcacag gggactcact gtgcagaggg ctctccgatc tggacatggg
      961 gcagggccag acagggattt tgcagcgtag tccacacctc gcggtcctct accacgcaga
     1021 gggtggtgga aacggtggtg gcggggacgc cctcgtgggg atgacggcag atacagttcg
     1081 gctcgttggt gtggtctgac ataatgcggt agagatcctg gaaccggagg gagccgtagc
     1141 gctcttccac catctgggca atgcggtcat agcgcttgtg agtggagggg tagttctcct
     1201 ccgggggatt gaaatggctg agctggtcac tgaccacgtg gttggtgcgg aagaggaccc
     1261 ccctgccctc atccttttcc acatagatct cgtcggaggt catctcgatg acataggcct
     1321 ctgtggggtc ggcggcaatg acgttcaggc cgttcatacg gggggcggtg cggcagaact
     1381 ccaccgcttg ggcggcggtc ctgcaggagg agaggaggac ccgcacattg aggaagatat
     1441 cggcctcctc cacccggtcg atgcgggtgg ggtccttctc tgtccagaag ccggtggtgg
     1501 tcacagccag accatggctg ttgatgccta cagcgggata ggtgaggata ccggccccgc
     1561 ccacctcgat gatctcctgg ccgtcggggt agatgtggcg gaccagagcc tgctccatgg
     1621 gcatgccca
//
LOCUS       DBMDSJ010000415         1625 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16384_length_1625_cov_2.148294, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000415 DBMDSJ010000000
VERSION     DBMDSJ010000415.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16384_length_1625_cov_2.148294"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="ACZPAQ_08760"
     CDS             complement(<1..828)
                     /locus_tag="ACZPAQ_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018028.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR0904120.1"
                     /translation="MTIKELERQTGLPRTTIRFYEQEGLIAPERRENNYRDYSEEDAA
                     TLEKIKLLRKLSLEVGTISRLKAGELTLSQALREQAAVLEADRGDLERYAQVCLEMSR
                     TEVDFGTLDPEPWLKELESWPLPPSRQVDLSRDSIAAAPYPWRRYFARGLDVGLCTCL
                     WMAVRYLVLEWYAPDQVLDGALRSLADMYGGWLFLFLLEPILLAAWGYTPGKWIMGLR
                     VRREDGRKLTWEEAGDRLVGVFFRGTGLGIPIYNIARLWDCYEKCAKDQVMSWDQGLS
                     "
     gene            complement(1004..1348)
                     /gene="rplQ"
                     /locus_tag="ACZPAQ_08765"
     CDS             complement(1004..1348)
                     /gene="rplQ"
                     /locus_tag="ACZPAQ_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR0904121.1"
                     /translation="MSLKHRKLGRTSDQRRAMLRAMVTYLLENGQIKTTITRAKEVAP
                     LAEKMITLAKQNDLAAYRQALGFITKEDVAKKLFQELGPKYAGRNGGYTRVVRIGPRR
                     GDAAEMAIIQLV"
     gene            complement(1502..>1625)
                     /locus_tag="ACZPAQ_08770"
     CDS             complement(1502..>1625)
                     /locus_tag="ACZPAQ_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIR0904122.1"
                     /translation="ISKTEEEMMKVRNLGRKSLEEVINKLAMMGLSLASDDSNG"
BASE COUNT          285 a          558 c          466 g          316 t
ORIGIN      
        1 gctcagcccc tggtcccaag acattacctg gtccttggca cacttctcat agcagtccca
       61 aaggcgggca atgttgtaga tagggatgcc cagccccgtc ccgcggaaga aaacgcccac
      121 cagccggtcg cccgcctcct cccatgtcag cttccggccg tcctcccgcc gtacccgcag
      181 ccccatgatc cacttgcccg gggtgtaacc ccaggcggcc agcaggatcg gctccaggag
      241 gaacaggaac agccagccgc cgtacatatc agccaggctt ctcagcgccc catccaggac
      301 ctggtcgggg gcataccatt ccagcaccag atagcgcacg gccatccaca gacaggtgca
      361 cagccctaca tccagccccc gggcaaagta tcttctccag gggtaggggg cggcggcaat
      421 ggagtcccga gacaggtcca cctgccggga gggcggcagg ggccagctct ccagctcctt
      481 gagccagggc tccgggtcca gcgtcccgaa gtccacctct gtccggctca tctccaggca
      541 cacctgggcg tagcgctcca gatccccccg gtctgcctcc agcacggcgg cctgctcccg
      601 cagggcctga gacagggtca gctctcccgc cttcagccgg ctgatggtcc ccacctccag
      661 gctcagcttc cgcagcagct tgatcttctc cagagtcgcc gcatcctcct ccgagtagtc
      721 acgatagttg ttctcccggc gctccggagc gatcaggccc tcctgttcat agaagcgaat
      781 agtggtccgg ggcaggccgg tctgccgctc cagctcctta atggtcatgg tcctccctcc
      841 tttgtcccca gtataaagta tgcaccaggt tgacagtcaa gaggaaattt cctttctcct
      901 ccttttttgc ccttccagca ccgcccccct ctccctgccc taaaagaaaa gagccccgcc
      961 tgctcggaaa gcaggcgggg actcactttg ggaaatcttg ctgttacacc agctggatga
     1021 tggccatctc agcggcgtcg ccgcggcggg gaccgatgcg gacaacgcgg gtgtagccgc
     1081 cgttgcggcc ggcatacttg gggcccagct cctggaacag cttcttggcc acatcctctt
     1141 tggtgatgaa gcccagagcc tggcggtaag cagccaggtc gttctgcttg gccagggtga
     1201 tcatcttctc ggccagagga gctacctcct tggcgcgggt gatggtggtc ttgatctggc
     1261 cgttctccag cagataggtc accatagcgc ggagcatggc ccggcgctgg tcactggtgc
     1321 ggcccagttt acggtgtttc agagacatat cagacactcc tatatttcgc gcggcgaaat
     1381 ttctgaattc gttcaggggc cggacggccc cggaggacac ccttgcgggg acagggagga
     1441 ccctgccagg cgaagcggaa cgttacatac ctccgccctg tcccgggagg cgggacgcct
     1501 ctcagccgtt gctgtcgtcg ctggccaggg acaggcccat catggccagc ttgttgatga
     1561 cctcttccag ggacttgcgg cccaggttgc gcaccttcat catctcttcc tcggtcttgg
     1621 agatc
//
LOCUS       DBMDSJ010000416         1612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16536_length_1612_cov_1.208471, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000416 DBMDSJ010000000
VERSION     DBMDSJ010000416.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16536_length_1612_cov_1.208471"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /gene="aspS"
                     /locus_tag="ACZPAQ_08775"
     CDS             <1..848
                     /gene="aspS"
                     /locus_tag="ACZPAQ_08775"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR0904123.1"
                     /translation="TEIHTPKIVHAGAEGGSNIFRLDYFGRKAFLAQSPQFYKQTMVG
                     SFQRVFEIGPVFRAEKHSTPRHLNEYTGLDLEMGFIDSFYDVMEVEAGFLKYCMELLA
                     REYSEDLKRLEAELPRVDRIPCVRFDEAKRLAAEKYGRPIRDPFDLEPEEEANIGRYF
                     QEEYGADFVFVTHYPSKKRPFYAMDDPEDPRCTLSFDLLFRGMEVTTGGQRVHDYQAQ
                     VAKMEARGMHPEEFRDFLTIHQHGMPPHGGLGIGLERLTMKLCGLENVREASLFPRDQ
                     SRLEP"
     gene            879..1166
                     /gene="gatC"
                     /locus_tag="ACZPAQ_08780"
     CDS             879..1166
                     /gene="gatC"
                     /locus_tag="ACZPAQ_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR0904124.1"
                     /translation="MKITTEMAAHIAALSRLKLSEEEAGRMAGELEQILSYMDVLDGL
                     DTSGAEPMSHIVPLKNVLREDETAPSMDRGELLKNAPATDGGTFLVPKAVE"
     assembly_gap    1279..1288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1289..>1612
                     /locus_tag="ACZPAQ_08785"
     CDS             <1289..>1612
                     /locus_tag="ACZPAQ_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR0904125.1"
                     /translation="EETAGDNAFTEITAERALERAAWAEKELAAGRLAGPLAGVPMAL
                     KDNICTRGVSTTCASRILAGFRPPYDATLVERLEGAGAVSLGKLNMDEFAMGSTSETS
                     FLGPVR"
BASE COUNT          299 a          497 c          565 g          241 t
ORIGIN      
        1 tcaccgagat ccacaccccc aagatcgtcc acgccggggc cgaggggggc agcaacatct
       61 tccggctgga ctacttcggg cgcaaggcat ttctcgccca gtccccccag ttctataagc
      121 agactatggt gggctccttc cagcgggtct ttgagatcgg ccccgtcttc cgggcggaga
      181 agcactccac cccccggcac ctgaatgagt acaccggcct ggacctggag atgggcttca
      241 tcgactcctt ctacgatgtg atggaggtgg aggccgggtt tttgaaatac tgcatggagc
      301 tgctggcgcg ggagtacagc gaggacctga agcggctgga ggcggagctg ccccgggtgg
      361 accgtatccc ctgcgtgcgg ttcgatgagg ccaagcggct ggccgcggag aagtatggcc
      421 gccctatccg ggaccccttt gacctggagc cggaggagga ggccaacatc ggacgctatt
      481 tccaggagga gtatggagcg gactttgtct ttgtcaccca ctaccccagc aagaagcgcc
      541 ccttctacgc catggacgac ccggaggacc cccgctgcac cctgtccttc gacctgctgt
      601 tccgggggat ggaggtgacc accggcggcc agcgggtcca cgactaccag gcccaggtgg
      661 ccaagatgga ggcccggggg atgcacccgg aggagttccg ggacttcctc accatccacc
      721 agcacggcat gccgccccac ggcggcctgg gcatcggcct ggagcggctg accatgaagc
      781 tgtgcggcct ggagaacgtg cgggaggcca gcctgttccc ccgggaccag agccgcctgg
      841 agccgtgagt ttgaaaacat gcagagaggt gaaacagaat gaagatcaca acggaaatgg
      901 cggcccatat cgccgccctc tcccggctga agctgtcgga ggaggaggcc gggcggatgg
      961 cgggggagct ggaacagatc ctctcctata tggatgtgct ggacggcctg gacaccagcg
     1021 gggcggagcc catgagccac atcgtccccc tgaagaacgt gctgcgggag gatgagacag
     1081 ccccctccat ggaccggggg gagctgctga agaacgcgcc ggccaccgac ggagggacct
     1141 tcctggtccc caaggccgtg gaataggagg gaaggacgat gcaggagatc atgaaactca
     1201 ccgcgctcca gctgggggcg gccatcaggc ggggcgaggt cagccccacg gaggcggtga
     1261 aggcggcgct ggagcgcann nnnnnnnntg aagagacggc gggggacaac gcctttactg
     1321 aaattacggc ggagcgggcc ctggagcggg ccgcctgggc ggaaaaggag ctggcggcgg
     1381 ggcggctggc gggccccctg gcgggggtcc ccatggccct caaggacaac atctgcacca
     1441 gaggggtgtc cactacctgc gcctcccgga tcctggccgg gttccgcccc ccttatgacg
     1501 ccaccctggt agagcggctg gagggggcgg gggccgtctc cctgggcaag ctgaacatgg
     1561 acgagttcgc catggggtcc accagcgaga cctctttcct ggggccggtg cg
//
LOCUS       DBMDSJ010000417         1605 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16617_length_1605_cov_1.724069, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000417 DBMDSJ010000000
VERSION     DBMDSJ010000417.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16617_length_1605_cov_1.724069"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            340..>1605
                     /locus_tag="ACZPAQ_08790"
     CDS             340..>1605
                     /locus_tag="ACZPAQ_08790"
                     /inference="COORDINATES: protein motif:HMM:NF039380.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR0904126.1"
                     /translation="MAAREDLKMRLTRLRGQTARLGRTGLSGPAVLRTAEVGIHLMLA
                     AVLAGAEVFGGFAPFGVAMVGASGTGLCGGAALLGAGFGYLTLLGFSDALRYLSAAIL
                     TFAVGFAFYDMKLFQRPWTMPGVAGLMNGCTGFIYLSQGGWRTVDVIYFLSEIALTVA
                     AAWCYRVLLLPIRRGSGEGVCSAARRTGALVLVCTTLISLSNLYLWKDVSFGRTLGAC
                     LVLAGAWQGGLAAGAVLGVTIGLSMDLAGSGVPLYAMAWGLAALAAGAFRGKKRVWAA
                     AAFAAADGAAVLWTWDRGLTLAILYEVFLGSVAFFMVPERQWKRLGGLLAPAPTAPPD
                     GRAQGYVQKKLEATAAAFRTLYESLRGSFRVPENDNDVAAVFDRAACRVCRTCSLRSS
                     CWERDYVTTFNALNDATAPMLERGRGVASD"
BASE COUNT          255 a          470 c          567 g          313 t
ORIGIN      
        1 cccagcggtc acaccagggt tccgccgaaa aggcatcccg gatcagtcct ttgacggcct
       61 gctcatgcgc catccccaat acttttaaca tcatttctgt tgacctccct ttctctgttt
      121 ccataaaaaa agccagactg aaacagcctg actgatggta ggtgctcacc caataggtct
      181 attgttataa tatcatattg cctggcaaaa agcaaccgga gggaaaaagg ccgccggagg
      241 aaatcgtctc ctgattccga ccatgttttt ccaaataatg gattataatg tggggcacaa
      301 cgttccggac aagccggaag ctacatagga ggagacgaga tggcggccag agaggatctg
      361 aaaatgcggc tgacgaggct gcgggggcag acggcccggc tggggcggac agggctgagc
      421 ggcccggcgg tgctgcggac ggcagaggtg ggcatccatc taatgctggc ggcggtgctg
      481 gcaggggcgg aggtgttcgg cggcttcgcc cccttcggcg tggccatggt gggggcatca
      541 ggcaccggac tgtgcggcgg ggcggccctg ctgggggcgg gattcggcta tctgaccctg
      601 ctgggcttct ctgatgcgct gcgctatctg tccgctgcca tcctgacctt cgcggtgggc
      661 tttgcatttt acgacatgaa gctcttccag aggccctgga ccatgcccgg ggtggcaggc
      721 ctgatgaacg gctgcaccgg cttcatctac ctgtcccagg ggggctggcg aacagtggac
      781 gtgatctact tccttagtga gatcgccctg acggtggccg cggcctggtg ctaccgggtg
      841 ctgctgctgc ctatacggcg ggggagcggg gagggggtat gctccgcagc ccggcggacc
      901 ggggccctgg tcctggtgtg taccaccttg atctccctct ccaacctgta tctatggaag
      961 gatgtctcct tcggccggac gctgggagcc tgtctggtgc tggcgggggc ctggcagggc
     1021 ggactggcgg caggagccgt acttggggtg accatcggct tgtccatgga tctggcgggc
     1081 tcgggcgtcc cactctacgc catggcctgg gggctggccg ctctggcagc aggggccttc
     1141 cggggcaaga aacgggtgtg ggcggcggcg gccttcgcgg cggcggacgg ggccgccgtc
     1201 ctgtggacct gggaccgggg gctgaccctg gccatcctgt atgaggtgtt tttgggttca
     1261 gtggcttttt tcatggtgcc ggagcgccag tggaagcgct tgggaggcct gctggcccca
     1321 gccccgacgg cgcccccgga tggcagggcc cagggctatg tgcagaaaaa gctggaggcc
     1381 accgccgccg ccttccgtac cttgtacgag tccctgcggg gctccttccg ggtgccggag
     1441 aacgacaacg atgtggcggc ggtctttgac cgggcggcct gtcgggtgtg ccggacctgc
     1501 tccctccggt ccagctgctg ggagcgggac tatgtgacca ccttcaacgc cctcaacgac
     1561 gccacggccc ccatgctgga gcgggggcgg ggagtggcca gcgac
//
LOCUS       DBMDSJ010000418         1604 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16630_length_1604_cov_1.793081, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000418 DBMDSJ010000000
VERSION     DBMDSJ010000418.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16630_length_1604_cov_1.793081"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="ACZPAQ_08795"
     CDS             <1..614
                     /locus_tag="ACZPAQ_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR0904127.1"
                     /translation="GLMDLKAVKVYARQLQEQIFPQLRVGLLYGKMKPREKEAVMSAF
                     ASGELQVLVSTTVIEVGVDVPNAALMIVENAERFGLSQLHQLRGRVGRGRHQSYCVLL
                     TSNRSPDTLARLRVFASTTDGFKISEEDLKLRGPGDFFGRRQHGLPTMRLADLSGDMR
                     LLSEAQQAARDLLSADPRLALGENRPVLERVRALFSDTPDIFN"
     gene            complement(658..>1604)
                     /locus_tag="ACZPAQ_08800"
     CDS             complement(658..>1604)
                     /locus_tag="ACZPAQ_08800"
                     /inference="COORDINATES: protein motif:HMM:NF037393.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5643 domain-containing protein"
                     /protein_id="HIR0904128.1"
                     /translation="WELQTPDGTPLEGYSSAQYSGTAGEAEELRHTEFHFSRGELPEE
                     FLAVCTVEPAGPAGEAENRAPAVDGAGEPAEGEAVALTFPIQLDRDKMAGPKTVVVDT
                     WVEADGQRLKVDQLEAYPTHTEIHLQEDPENTCWLAGAEFAFVDGNGKEYDTGDGYIT
                     ATGGEDSRSMLHYYRQSLYFLEGQEMTLSIRRLKWLDKEQEWADIDLAAGTACGLPEG
                     TELLQVCREGDRTILLFRQAEGGPDAPFTFLFRDPEGTEHTFDGVTTSGTAEEVDGRK
                     HVEYLYHIQNCAWDRIQIQLAHTETADHEDLNIPFVLP"
BASE COUNT          245 a          562 c          488 g          309 t
ORIGIN      
        1 ccggcctgat ggacctgaag gcggtgaagg tctacgcccg gcagctccag gagcagatct
       61 ttcctcagct gcgggtgggg ctgctctacg gtaagatgaa gccccgggag aaggaggccg
      121 tcatgtccgc cttcgccagt ggggagctcc aggtcttggt gtctaccacc gtcatcgaag
      181 tgggggtgga cgtgcccaac gccgccctta tgatcgtcga aaacgcagag cgcttcggcc
      241 tcagccagct ccaccagctc cggggcaggg tgggccgggg ccggcaccag tcctactgcg
      301 tgctcctcac ctccaaccgc agcccggaca ccttggcccg tctgcgggtc ttcgcctcca
      361 ccacagacgg cttcaagatc tctgaggagg acctgaagct acggggccct ggcgacttct
      421 tcggccgccg ccagcatggc ctgcccacca tgcggctggc cgatctctcc ggggatatgc
      481 gcctgctcag cgaggctcag caggcggcac gggacctgct ctccgccgac ccccgcctgg
      541 ccttggggga aaaccgccct gtgctggagc gagtacgggc actgttctcc gatacgccgg
      601 acatcttcaa ctaaaacagc agaccggggc cgcggctcaa gccgcggccc cggtctgtca
      661 cggcaggaca aaggggatgt tcaggtcttc atggtccgcc gtctctgtat gtgccagctg
      721 gatctggatg cgatcccagg cacagttttg gatgtgatac agatactcga catgcttcct
      781 cccgtccacc tcctccgctg tcccagaggt ggtcacgccg tcgaatgtgt gctccgtccc
      841 ctccgggtcc cgaaagagga aggtgaaggg ggcgtccggc cctccctccg cctgccggaa
      901 aagcaggatg gtgcggtccc cttcccggca gacctgaagc agctcagtcc cctccggaag
      961 tccgcaggcg gttccggcgg ccaggtcgat gtccgcccac tcctgctcct tgtccagcca
     1021 tttcagcctc cggatggaga gggtcatctc ctgtccctcc aggaaataca ggctctgccg
     1081 gtaatagtgg agcatggagc gggaatcctc cccgccggtg gcagtgatat acccgtcccc
     1141 tgtgtcgtat tcttttccat tcccgtctac aaaggcaaac tcggcccccg ccagccagca
     1201 ggtattttcc gggtcctcct ggagatggat ctccgtgtgg gtggggtagg cctccagctg
     1261 gtccaccttc agccgctggc cgtccgcctc cacccaggtg tccaccacca ccgtcttggg
     1321 accggccatc ttgtcccggt ccagctggat ggggaacgtg agggccacag cttccccctc
     1381 cgccggctct ccggccccgt ccactgctgg ggccctgttt tccgcttccc ctgctggtcc
     1441 ggccggttcc acggtgcaca cggccaggaa ctcctccggg agttcccccc ggctgaaatg
     1501 gaactccgtg tgccgcaatt cctccgcctc tccggccgtt ccgctgtact gggcggagct
     1561 gtatccttcc aggggggtgc cgtccggcgt ctggagctcc cagg
//
LOCUS       DBMDSJ010000419         1602 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16649_length_1602_cov_1.488341, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000419 DBMDSJ010000000
VERSION     DBMDSJ010000419.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16649_length_1602_cov_1.488341"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="ACZPAQ_08805"
     CDS             <1..612
                     /locus_tag="ACZPAQ_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0904129.1"
                     /translation="QLDHHPDPTKSRGQYLPLLELSVEEEPEDDRNTHYLGREYMYRG
                     RWDDCIRTLEHHLSMPTATWKDERAASMRYIALSWLRKGDRARARDWYLRAIAEAPHL
                     REPYMDLARMLYDMEEWDGVLYFTGCALSITIRPKTYICEADSWGSLPHDLRCQALFQ
                     TGRRALALDEARAALACAPSDPRLRGNVAVLEQLLGETERSAP"
     gene            complement(689..>1602)
                     /locus_tag="ACZPAQ_08810"
     CDS             complement(689..>1602)
                     /locus_tag="ACZPAQ_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0904130.1"
                     /translation="LVGLIPRTAGEIALLVVYILLGVDFVGSWAAVLQLHGALSEPTH
                     ISRFLRRLTHTLDTTVTRHIQRRMERAYPSLERERLSGKQAPKVKPERFAQGCGFYKL
                     AALFFIGSFLGDITETIWCWAVLGKLMSRSSVLYGPFSIVWGLGCVLLTALLYKYKDR
                     SDRYIFLAGTVLGGAYEYSCSVFTELVFGTVFWDYSHIPFNLGGRINLLFCFFWGIAA
                     VVWLKGLYPRLSAWIERIPIRWGKAGTWVLIVFMVCNIIISALALDRYTQRSAGDAPQ
                     TTLGLFLDRHYPDQRIERVYPNAKIVD"
BASE COUNT          331 a          496 c          488 g          287 t
ORIGIN      
        1 cagctggacc accaccccga ccccacaaag tcccggggac agtacttgcc tctgctggaa
       61 ctgtccgtgg aggaggagcc ggaggatgac cggaacaccc actatttggg ccgggagtac
      121 atgtaccggg gccgctggga cgactgcatc cgcaccctgg agcaccacct gtccatgccc
      181 accgccacct ggaaagatga gcgggcggcc tccatgcggt atatcgccct gtcctggctc
      241 cgaaagggag accgggcccg agcacgggac tggtatctcc gtgccattgc agaggccccc
      301 catctgcggg agccgtatat ggatctagcc aggatgctgt atgacatgga ggaatgggat
      361 ggagtgctct actttaccgg ctgtgccctg tccatcacta tccgcccaaa aacctatatc
      421 tgtgaggcgg actcctgggg cagcttgccc cacgaccttc gctgtcaggc cctttttcag
      481 actgggcgga gggccttggc gctggatgag gccagggctg ccctcgcctg tgctccatcg
      541 gacccgcggc tgcggggcaa tgtagcggtg ctggaacagc tgcttggaga gacggagagg
      601 tccgccccct aaggggcgga cctcagcccg cttccgggcg ctgttatcct gggacattct
      661 ccctctcagg gggcaggcgg ccgtcctctc agtccacgat cttggcgttg gggtagaccc
      721 gctcaatccg ctggtcgggg taatggcggt ccaggaagag ccccagagta gtctgggggg
      781 cgtccccggc gctgcgctgg gtgtatcggt ccagtgccag ggcagatatg atgatgttgc
      841 acaccataaa gacgataagc acccaggtgc ccgcctttcc ccagcggatg gggatccgct
      901 cgatccacgc tgagagtctg ggatagaggc ccttcagcca taccacggcc gcaattcccc
      961 agaaaaagca aaacagcaga ttgatccgtc ccccaaggtt gaagggaata tggctgtaat
     1021 cccagaagac ggtaccgaac accaactcag taaagacgga gcagctgtac tcgtatgcgc
     1081 cccccagcac tgtgccggcc aggaaaatat atcggtcgct gcggtccttg tatttgtaga
     1141 gcagcgcagt gagcaggaca cagcccagcc cccagacaat ggagaagggg ccgtagagca
     1201 cactgctgcg gctcatcagc ttccccaaca ctgcccagca ccaaatagtt tcggtgatat
     1261 cccccaggaa agaaccgatg aagaacagcg cggccaactt gtaaaagccg catccctggg
     1321 caaaccgctc cggcttgacc tttggagcct gctttccgct cagccgttcc cgctccaggc
     1381 tggggtatgc cctttccata cgtctctgga tgtgtcgggt cacggtggta tctagggtat
     1441 gggtaagccg gcgcaggaag cgggagatat gggtaggctc gctcagggcg ccgtggagct
     1501 ggagcacagc ggcccaggag ccaacaaagt ccactcccag cagaatatac accaccagca
     1561 gtgcgatctc tcccgccgtc ctggggatca ggcccaccaa tg
//
LOCUS       DBMDSJ010000420         1600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16672_length_1600_cov_2.146097, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000420 DBMDSJ010000000
VERSION     DBMDSJ010000420.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16672_length_1600_cov_2.146097"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="ACZPAQ_08815"
     CDS             complement(<1..643)
                     /locus_tag="ACZPAQ_08815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0051117 - ATPase binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP family protease"
                     /protein_id="HIR0904131.1"
                     /translation="MEENQQGEQQEMEQAIQPIVEMGSSTIRTGRGTVHVLTIVGQIE
                     GHQLLPPTSKSTKYEHVMPLLAMVEESEEVDGLLVLLNTVGGDIEAGLGIAELIASMS
                     KPTVSLVLGGGHSIGVPLAVSAKRSFIAPSAAMTIHPVRLNGLVIGVPQTFYYFERIQ
                     ERILQFVTANSSIKRETFTKLMLQTGELAADVGSVIYGEEAVELGLIDRIGGLS"
     gene            786..1358
                     /locus_tag="ACZPAQ_08820"
     CDS             786..1358
                     /locus_tag="ACZPAQ_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0904132.1"
                     /translation="MDNIRFGSFVAQLRKERGLTQKELAQRLHVTDKAVSKWETGRGF
                     PDLKLLEPLAQTLEVSLVELLQGERTSSPTLSVEEAGQVAARAMDQYQQTTVLRYLRL
                     FRWVCTAAAVFCFLFLLPYLVYGVSQLYFHWVIEPQMGVIGSAEGPTVIITSSAPLFP
                     AWFRPALLALLCAACGVLVFRLHCLERRLK"
     gene            1355..>1600
                     /locus_tag="ACZPAQ_08825"
     CDS             1355..>1600
                     /locus_tag="ACZPAQ_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904133.1"
                     /translation="MKRLFPLLLTLLLFPLPGCGLGNGIPRGLDWQALTVTDQKGGAL
                     LSAAPGWEGGKDVPRLSLSVQVEADSVVLTRPETGERW"
BASE COUNT          267 a          530 c          470 g          333 t
ORIGIN      
        1 ctgacaagcc gccgatccgg tcaatcagcc ccagctccac cgcctcttca ccgtagatca
       61 cgctgcccac gtcggcggcc agctctccgg tctggagcat cagctttgta aaggtctccc
      121 tttttatgga actgttggca gtcacaaatt gcaggatacg ctcctggatg cgctcaaaat
      181 aataaaaggt ctggggcacg ccgatcacca gcccattcag ccgcaccgga tggatagtca
      241 tggcggcgga tggggcgata aaggagcgct tggccgacac agccagcggc acgccgatgg
      301 agtgccctcc gcccagcacc agggacaccg tgggcttgga catgctggcg atcagctccg
      361 cgatgcccag ccctgcctcg atatccccgc ccaccgtgtt cagcaggacc agaagtccgt
      421 ccacctcctc gctctcctcc accatggcca gcagcggcat cacatgctca tacttcgtgc
      481 tcttgctggt gggaggcagc agctgatgtc cctcgatctg ccccacgatg gtgaggacat
      541 ggactgtacc gcggccggtc cgaatggtgg aggaccccat ctccacgatg ggctggatgg
      601 cctgctccat ctcctgctgc tccccctgct ggttctcctc cataatgcat tcctcctttt
      661 tcagaagtag cttgcgccga agtccggaat tttactcctg attttccctc attggccctg
      721 tctacttttc gtaggttgac ttttttccgc cccatgtcat aataaaatag aaaaggaggg
      781 gctgtatgga caacatacgc tttgggtcct ttgtggccca gctgagaaag gagcgggggc
      841 tgacccagaa ggagctggcc cagcggctcc acgtgacgga caaggccgtc tcaaaatggg
      901 agacaggacg gggatttccc gacctgaagc tgctggagcc cctggcccag accctggagg
      961 tctctctggt ggagctgctc cagggggagc ggacctcttc ccccactctg tccgtagagg
     1021 aggcgggaca ggtggcggcc cgcgctatgg accagtacca gcagaccacc gtcctgcgct
     1081 atctccggct gttccggtgg gtctgcaccg ccgcggcggt cttctgcttc ctctttctgc
     1141 tgccgtacct ggtttacggc gtctcccagc tctactttca ttgggtgatc gagccccaga
     1201 tgggggtcat cggcagcgcg gagggcccca ccgttatcat cacttcctcg gctcccctgt
     1261 tccccgcctg gttccggccg gcactgctgg ccctcctgtg cgccgcctgc ggcgtactgg
     1321 ttttccggct ccactgcctg gagaggaggc tgaaatgaag cgcctgttcc ctctcctgct
     1381 gacgctgctc ctgttccccc tgcccggctg tgggctgggg aatgggatcc cccgcggtct
     1441 ggactggcag gccctcaccg tcactgacca gaagggaggc gcgctgctct ccgccgcccc
     1501 aggctgggaa gggggaaagg acgttccccg tctctccctg tccgtccagg tggaggcaga
     1561 cagcgtggtc ctcacccggc cggagaccgg agagcgatgg
//
LOCUS       DBMDSJ010000421         1598 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16707_length_1598_cov_1.462926, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000421 DBMDSJ010000000
VERSION     DBMDSJ010000421.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16707_length_1598_cov_1.462926"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="ACZPAQ_08830"
     CDS             <1..964
                     /locus_tag="ACZPAQ_08830"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR0904134.1"
                     /translation="RLQDSYEEERLGGKAFGSTKSGIAPFYSDKYAKFGIQVCDLFHE
                     DRLRERLTAAVSLKNVLFEHLYHKPSLDVDELYQQLMELREQIRPYVGDAVTFVHDAL
                     RAGKTVLLEGQLGSMKDPDLGIFPLTTSSHTLAGFGCVGAAVPPTAVEDVICVTKAYS
                     SSVGSNTEPFVSEVLGEAGDELRRRGGDKGEFGATTGRPRRVGWFDTVATKYGCMVQG
                     ATQVALTCLDVLGYLDEIPVCTGYEIDGTVTDRFPTTPELMRARPVFTTLPGWKCDIR
                     GCTDYQALPQQAKAYVDFLESRIGYPITLVSTGPKRNEITVRQK"
     gene            1078..>1598
                     /locus_tag="ACZPAQ_08835"
     CDS             1078..>1598
                     /locus_tag="ACZPAQ_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR0904135.1"
                     /translation="MTKIDIISGFLGAGKTTLIKKLLSESFHGEKVVLIENEFGEIGI
                     DGGFLKDAGVQITEMNSGCICCSLVGDFGTALKEVLDTYSPDRIIIEPSGVGKLSDVI
                     AAVERVRDGGAPLELHSFVTVVDAVKCKIYMKNFGEFFNNQVEHASAILLSRTQNMDQ
                     AKLAADVAMLRPH"
BASE COUNT          285 a          540 c          483 g          290 t
ORIGIN      
        1 ccgtctccag gacagctacg aggaggagcg cctgggcggc aaggcctttg gctccaccaa
       61 gagcggcatc gcccccttct actcggacaa atacgccaag ttcggcatcc aggtctgcga
      121 cctgttccac gaggaccggc tgcgggagcg cctgaccgcc gccgtctccc tgaaaaatgt
      181 gctgttcgag cacctgtacc acaagccctc cctggacgtg gacgagctgt accagcagct
      241 gatggagctg cgggagcaga tccgccccta tgtgggggac gccgtcacct ttgttcacga
      301 cgccctgcgg gcgggcaaga ccgtcctgct ggagggccag ctgggctcta tgaaggaccc
      361 tgacctgggt atcttccccc tgaccacctc ctcccacacc ctggccggct tcggctgcgt
      421 gggggccgcc gtccccccca ccgctgtgga ggacgtgatc tgcgtcacca aggcgtactc
      481 ctcctctgtg ggctccaaca ccgagccctt cgtcagcgag gtgctgggcg aggcgggaga
      541 tgagctgcgc cgccgcggcg gtgacaaggg cgagttcggc gccaccaccg gccgtccccg
      601 ccgggtaggc tggttcgaca ctgtggccac caagtacggc tgcatggtcc agggggccac
      661 ccaggtggct ctgacctgcc tggacgtact gggctatctg gatgagatcc cggtgtgcac
      721 cggctatgag atcgacggga cagtcaccga ccgcttcccc accaccccgg agctgatgcg
      781 ggccaggccc gtgttcacca cgctccccgg ctggaagtgc gacatccggg gctgcaccga
      841 ctaccaggcc ctgcctcagc aggcaaaggc ctatgtggac ttcctggagt ccaggatcgg
      901 ctatcccatc accctggtct ccaccggacc caagcgcaac gagatcaccg tccggcagaa
      961 gtaatttccc ggactgctat gcgccggata ccaggctctg gtatccggcg ctcccccttc
     1021 cccctctgtc catccggcgg ccctgccgcc ttgtttgaag aaaggatctg tgtcaccatg
     1081 accaaaatcg acattatctc cgggtttttg ggtgccggca agaccacgct catcaagaag
     1141 ctcctctccg agtccttcca tggcgagaaa gtggtcctga tcgagaacga gttcggtgag
     1201 atcggcatcg acggcggctt tctgaaggat gccggcgtcc agatcacgga gatgaattcc
     1261 ggctgcatct gctgctccct ggtaggggat ttcggcaccg ccctcaagga ggtgctggat
     1321 acctattcgc ccgaccgcat catcatcgag ccctccggcg tgggcaagct gtccgatgtc
     1381 atcgccgccg tggagcgggt gcgggacggc ggtgcccccc tggaactgca cagctttgtc
     1441 accgtggtgg acgcggtgaa gtgcaagatc tatatgaaga actttggcga gttcttcaac
     1501 aaccaggtgg agcacgcctc cgctatcctc ctcagccgga cccagaacat ggaccaggcc
     1561 aagctggccg ccgacgtagc catgctgcgt ccccacaa
//
LOCUS       DBMDSJ010000422         1592 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16787_length_1592_cov_2.007378, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000422 DBMDSJ010000000
VERSION     DBMDSJ010000422.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16787_length_1592_cov_2.007378"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="ACZPAQ_08840"
     CDS             complement(<1..366)
                     /locus_tag="ACZPAQ_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HIR0904136.1"
                     /translation="MPLDALCLSGVVHELQNALSGAKIDKIYQPGRDEVVLALRAPAG
                     NVKLLLSANPSHPRAHLTQISRENPDKPPMFCMLLRKHLAGARLLELVQPPMERVVDL
                     RLEALDELGDRVERRLVLEA"
     gene            complement(401..808)
                     /locus_tag="ACZPAQ_08845"
     CDS             complement(401..808)
                     /locus_tag="ACZPAQ_08845"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HIR0904137.1"
                     /translation="MIDYFHLNMDAEQLLNLSSLGLAHLGDGVYELMVRSWLCLHGKA
                     TARNLHRATVGYVAAPAQALRAERILPLLTQEEADVFRRGRNTAPHSVPKGASRGEYQ
                     TATALEALFGWLYLQGRTDRLNQLFSTMMEGEL"
     gene            1010..1513
                     /locus_tag="ACZPAQ_08850"
     CDS             1010..1513
                     /locus_tag="ACZPAQ_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR0904138.1"
                     /translation="MHNELTQKDLQLMREELDYRRVQLRPKLLEAVKEARAFGDLSEN
                     FEYKAAKQEKNRNESRIRFLENMIKTAVVIADHSGADTVGLYDRVTVYLEEDDETEVY
                     QIVTTMRQDALHGLVSKESPMGKALLGRRVGDRPYVQVNEQYGYYVEVRSIEKGTDDG
                     SVPLNRF"
BASE COUNT          316 a          476 c          548 g          252 t
ORIGIN      
        1 ggcctccagc accaggcggc gctccacccg gtcccccagc tcgtccaggg cctccagccg
       61 caggtccacc acccgctcca tgggtggctg gaccagctcc agcaggcggg cgccggccag
      121 gtgcttgcgc agcagcatgc agaacatggg gggcttgtcc gggttctccc gggagatctg
      181 ggtgagatgg gcccgggggt ggctggggtt ggcggagagg agcagcttca cattgcctgc
      241 gggggcccgc agggccagga ccacctcgtc ccggcctggc tgatagatct tgtcgatttt
      301 tgccccggag agggcgttct gcagttcatg gaccactccg ctcaggcaca gcgcgtccaa
      361 aggcatggga tcacctcatt tatgtcacag gttgggcggg tcacagctcc ccctccatca
      421 tggtggagaa gagctggttg agccggtcgg tccggccctg gagatagagc cagccgaaca
      481 gggcctccag ggcggtggcg gtctgatact ccccacggct ggcccccttg gggacagagt
      541 ggggggcggt gtttcggccc cggcggaaga catccgcctc ctcctgggtg agcaggggga
      601 ggatgcgctc tgcccgcagg gcctgggccg gcgcggccac atagcccact gtggcccggt
      661 gcaggttgcg ggcggtggcc ttgccgtgga gacagagcca ggagcgcacc atcagctcat
      721 acaccccgtc ccccaggtgg gccagcccca ggctggacag attcagcagc tgttcagcgt
      781 ccatattcag gtggaagtag tcgatcatag ggtcccctcc gtctcaggct ttcaatctat
      841 catcatacca cactcccccc tataagacaa tgcctatatg ctgtaaagat cacatgcgca
      901 cacacgagag caaagagagc tggtgcaggc cgttgccaac cgggccgggg tctggtacaa
      961 tagggccaaa agaaaagggc cccgggacgg ggcggagagg agaagcgcca tgcacaacga
     1021 actgacgcag aaggacctcc agctgatgcg ggaggagctg gactaccgca gggtccagct
     1081 gcggcccaaa ctgctggaag cggtgaagga ggccagggct ttcggcgatc tgagcgagaa
     1141 ttttgagtac aaggcggcca agcaggagaa gaaccggaac gagagccgca tccgatttct
     1201 ggaaaacatg atcaagaccg ctgtggtcat cgcggaccat tccggggcgg acacggtggg
     1261 attgtacgac cgggtgacgg tctacctgga ggaggacgac gagacagagg tgtaccagat
     1321 cgtcaccacc atgcgccagg atgccctcca cggcctggtg agcaaggagt cccccatggg
     1381 aaaggccctg ctgggccgca gggtggggga ccggccctat gtccaggtca atgagcagta
     1441 cggctactat gtggaggtca gatccatcga aaaggggacg gacgacggct ccgtccccct
     1501 caaccgattc tgagcggcgg gagagcaggg aaaggggggc acggcgcaag ccgtgccccc
     1561 cagcttttcc ggcggtgtat cctcacaggt gg
//
LOCUS       DBMDSJ010000423         1590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16806_length_1590_cov_1.896575, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000423 DBMDSJ010000000
VERSION     DBMDSJ010000423.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16806_length_1590_cov_1.896575"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1590
                     /locus_tag="ACZPAQ_08855"
     CDS             <1..>1590
                     /locus_tag="ACZPAQ_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR0904139.1"
                     /translation="SDILAEEISDDADIRRELRQLYLRRGMLASRAAAKEPEDTVYRL
                     YYDFKVPVGRAQGHQVLAVNRGEREGVLKVTVELDPETAHVAVRRAVVPGAACMDFVR
                     AAADDAYDRLIQPSMEREIRAHLTEQADEGAIHNFALNLKPLLMQPPVKGRVTMGLDP
                     GYRMGCKVAVVDGTGKVLDTAVVYPTYGERQKQEAIAKLSHLVRKHGVEHIAIGNGTA
                     SRETEQMAVELIHRLGGGLSYMIVSEAGASVYSASKLAAEEFPEYDVNLRSAVSIARR
                     LQDPLAELVKIDPKAIGVGQYQHDMPQARLGEALDGVVEDCVNAVGVDVNTASPSLLQ
                     RVSGLTAATAKNLVKYREENGAFTTRRQVLKVPKLGPKAFEQCAGFLRVPESASVLDN
                     TGVHPESYAAAEALLAQCGYTLSDVRAGAMGALKEKAEAMGLEALARVCGVGVPTLAD
                     IIKELMKPGRDPRDELPAPILRTDVMEARDLKPGMELQGTVRNVIDFGAFVDIGVHQD
                     GLVHISKLPQRVKHPSQLLSVG"
BASE COUNT          268 a          521 c          577 g          224 t
ORIGIN      
        1 agtgacatcc tggccgagga gatctccgac gacgccgaca tccgcaggga gctgcggcag
       61 ctgtatctgc gccggggcat gctggcctcc cgggctgcgg ccaaggagcc ggaggacacg
      121 gtctaccgcc tgtactatga cttcaaggtc ccggtgggac gggcccaggg ccaccaggtg
      181 ctggccgtca accggggtga gcgggagggg gtgctcaagg tcacggtgga gctggacccg
      241 gagaccgccc acgtggcggt gcgccgggcc gtggtgcccg gcgcggcctg catggacttt
      301 gtccgggcgg cggcggacga cgcctatgac cgcctgatcc agccctccat ggagcgggag
      361 atccgcgccc acctcactga gcaggcggac gagggtgcca tccacaactt cgccctgaac
      421 ctgaagcccc tgctgatgca gcccccggtg aaggggcggg tcaccatggg gctggacccg
      481 ggctaccgca tgggctgcaa ggtggcggtg gtggacggca ccggcaaggt gctggacacg
      541 gcggtagtct accccaccta tggggagcgg cagaaacagg aggccatcgc caagctgtcc
      601 catctggtga gaaagcacgg cgtggagcac atcgccatcg gcaacggcac cgccagccgg
      661 gagaccgagc agatggcggt ggagctgatc cacagactgg ggggcggcct gagctatatg
      721 atcgtcagcg aggccggggc ctcggtctac tccgcctcca agctggccgc cgaggagttc
      781 cccgagtacg atgtgaacct gcgctccgcc gtgtccatcg cccggcggct ccaggaccct
      841 ctggctgagc tggtgaagat cgaccctaag gccatcggcg tgggccagta ccagcacgac
      901 atgccccagg cccggctggg cgaggccctg gacggggtgg tggaggactg cgtcaacgcg
      961 gtgggcgtgg atgtgaacac cgcctccccc tccctgctcc agcgggtgtc cggcctcacc
     1021 gccgccactg ccaaaaacct ggtgaaatac cgggaggaga acggggcctt caccacccgc
     1081 aggcaggtcc tgaaggtgcc caagctgggc cccaaggcct ttgagcagtg cgccggcttc
     1141 ctgcgggtgc cggagagcgc ctccgttctg gacaacaccg gtgtccatcc ggagagctac
     1201 gccgccgcgg aggcgctgct ggcccagtgc ggctacaccc tgtccgacgt cagggcaggg
     1261 gccatggggg ccctgaagga gaaggcggag gccatgggcc tggaggcgct ggcccgggtg
     1321 tgcggcgtgg gcgtccccac cctggcggac atcatcaagg agctgatgaa gcccggccgg
     1381 gacccccggg acgagctgcc cgcccccatc ctgcgcaccg atgtgatgga ggcccgggac
     1441 ctgaagcccg ggatggagct ccaggggacg gtgcgcaacg tcatcgactt cggggcattt
     1501 gtggacatcg gggtccatca ggatgggctg gtccacatca gcaagctgcc ccagcgggtg
     1561 aagcacccct cccagctgct ttccgtggga
//
LOCUS       DBMDSJ010000424         1581 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16910_length_1581_cov_1.805405, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000424 DBMDSJ010000000
VERSION     DBMDSJ010000424.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16910_length_1581_cov_1.805405"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1581)
                     /locus_tag="ACZPAQ_08860"
     CDS             complement(<1..>1581)
                     /locus_tag="ACZPAQ_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR0904140.1"
                     /translation="YPPLSLLKEGPGDIGGEALVELNANRQRLDDTIHSFGIDANIVN
                     VTRGPSVTRYELELDQGVRLNKLTNLADDIALALGASGVRIAPIPDQISMVGIEVPNK
                     LVSPVNIHSVIGSREFTDSPSKVSFAVGKDIGGHCIVGNIAKLPHLLIAGTTGSGKSV
                     CTNSLIISLLYKATPDEVRLIMVDPKMVELGIYNGIPHLLIPVVTDPKKAAGALQWAV
                     TEMMKRYRTFAEVGVRDLAAYNALAARTEGMTKMPQIVVVIDELADLMLVAAKEVEES
                     ICRVAQMGRASGMHLIIATQRPSADVITGLMKANIPSRIAFAVASSLESRIILDTTGA
                     EKLVGRGDMLYFPLGSGKPTRVQGCLISDQEVAAVVDFVKQNSGDASYDDQVMQEIEQ
                     HAAEKEKGAKGVGGSAPEEVGEEFDELIDAAAEVILETGQASVSMLQRRLKLGYARAA
                     RLVDQLEEKGIVGPFEGSKPRQLLITKEQWQEMKYRQGVPGGTPPESASGAAPGGAEE
                     DMPPFDVGEALDRAEEDTL"
BASE COUNT          229 a          534 c          530 g          288 t
ORIGIN      
        1 cagggtgtcc tcctccgctc ggtccagggc ctctcccaca tcgaaggggg gcatgtcctc
       61 ttccgctccg cctggagctg ccccggaagc gctctcggga ggggttcccc cggggacgcc
      121 ctggcggtac ttcatctcct gccactgctc cttggtgatg agcagctggc ggggctttga
      181 gccctcgaag ggccccacga tccccttctc ctccagctgg tccaccagcc gggccgcccg
      241 ggcatagccc agcttcaggc ggcgctggag catagagacc gacgcctgtc ccgtctccag
      301 gatcacctcg gctgccgcgt cgatgagctc gtcgaactcc tcccccacct cctcgggggc
      361 ggagccgccc acgcccttgg cgcccttctc cttctcggcg gcgtgctgct cgatctcctg
      421 catcacctgg tcatcgtagc tggcgtcgcc gctgttctgc ttgacaaagt ccaccactgc
      481 ggccacctcc tggtcggaga tgaggcagcc ctggacccgg gtgggcttgc cggagcccag
      541 ggggaagtag agcatgtcgc cccggccaac cagcttctcc gcgccggtgg tgtccaggat
      601 gatgcgggac tccaggctgg aggccaccgc gaaggcgatg cggctgggga tattggcctt
      661 catcaggccg gtgatcacgt cggcggaggg ccgctgggtg gcgatgatga ggtgcatgcc
      721 ggaggcgcgg cccatctggg ccacccggca gatggactcc tccacctcct tggccgccac
      781 cagcatcagg tcggccagct cgtcgatgac caccacgatc tggggcattt tggtcatgcc
      841 ctccgtcctg gcggccaggg cgttgtaggc ggccagatcc cgcaccccaa cctcggcaaa
      901 ggtgcggtag cgcttcatca tctcggtcac cgcccactgc agggcgcccg ccgccttctt
      961 ggggtcagtg accaccggga tcagcaggtg ggggatgccg ttgtagatgc ccagctccac
     1021 catcttgggg tccaccatga tcaggcgcac ctcgtcgggg gtggccttat agagcagtga
     1081 gatgatcagg gagttggtgc acaccgactt gcccgagccg gtggtgccgg caatgagcag
     1141 gtggggcagc ttggcgatgt tgcccacgat gcagtggccg ccgatgtcct tgcccacggc
     1201 gaaggacacc ttggaggggc tgtcggtgaa ctccctggag ccgatgacgc tgtggatgtt
     1261 caccggcgac accagcttgt tgggcacctc gatgcccacc atggagatct ggtccgggat
     1321 gggggcgatg cgcacgccgg aggcccccag ggccagggcg atgtcgtccg ccagattggt
     1381 cagcttgttc agccggacgc cctggtccag ctccagctcg taccgggtca ccgaggggcc
     1441 ccgggtcacg ttgacgatgt tggcgtcgat gccgaaggag tggatggtgt catccagccg
     1501 ctgccggttg gcgttgagct ccaccagggc ctccccgccg atgtcccccg gtccctcctt
     1561 cagcaaggag agggggggat a
//
LOCUS       DBMDSJ010000425         1580 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16924_length_1580_cov_1.440838, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000425 DBMDSJ010000000
VERSION     DBMDSJ010000425.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16924_length_1580_cov_1.440838"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="ACZPAQ_08865"
     CDS             complement(<1..817)
                     /locus_tag="ACZPAQ_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904141.1"
                     /translation="MKWRLCAAVAALSLLLSGCSSLLARSYTSVTPHSATPPAEGDSS
                     ILRVENYQELVNALIYLISLGEEEGTVRMYNYDQDVEQSLSNACLEVVQEDPLGAYSV
                     DFIRYDVTPIVSYYEAAVEITYRRTREQVSAIVAATGATAIRSQLKDLLSSFGTEAAL
                     RISYFEGDETYIQTLFREAYYASPDTALDLPEAQVYIYPQGEESGRQRIVEVLLTYHL
                     EQKELQRRRTALARRANEIVVSIWGTEGDEAIQTVSAAVLDAGHYDPEGGGSAY"
     gene            complement(849..1439)
                     /locus_tag="ACZPAQ_08870"
     CDS             complement(849..1439)
                     /locus_tag="ACZPAQ_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HIR0904142.1"
                     /translation="MADLEKLRANLEARGFRTTYFPTADEAAAYLDGQIDGKTVGIGG
                     SMTVKEMGLYPRLAAHNQVVWHWEGGALRDAVLTDVYITSANALAETGEIVNIDGNGN
                     RVSSTLYGHQKVYFLVGVNKVAPDFEAALWRARNVAAPKRAQSMGCRTPCAAKADRCY
                     DCKSPERICRGLAVLWARPNATEEMEVVLIGEALGM"
     gene            complement(1469..1576)
                     /locus_tag="ACZPAQ_08875"
     CDS             complement(1469..1576)
                     /locus_tag="ACZPAQ_08875"
                     /inference="COORDINATES: protein motif:HMM:NF021546.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR0904143.1"
                     /translation="MKRAVKETDPSAFLIVCDAHEVLGQGFHSYHQHDL"
BASE COUNT          270 a          499 c          522 g          289 t
ORIGIN      
        1 cataagcgct gccccccccc tccggatcgt agtgtccggc gtccagcaca gcggcagata
       61 cggtctggat ggcctcgtcc ccctcggtcc cccagatgga gaccacgatc tcattggcgc
      121 gccgggccag ggcggtccgg cggcgctgga gctctttctg ctccaggtgg taggtaagca
      181 ggacctccac gatgcgctgg cggccgctct cctccccttg gggatagata tagacctggg
      241 cctcgggcag atccagggcg gtgtccggac tggcatagta ggcctcccgg aagagggttt
      301 ggatgtaggt ctcgtccccc tcgaagtagc tgatgcgcag ggcggcctcg gtgccgaagg
      361 aggagagcag gtccttgagt tgggagcgga tggcggtggc accggtggcg gccacgatgg
      421 cggagacctg ctcccgggtg cggcggtagg tgatctccac agcggcctca tagtaggaga
      481 caataggggt cacatcatag cggatgaagt ccactgaata ggctcccaga gggtcctcct
      541 ggaccacctc cagacaggcg ttggacaggg actgctccac atcctggtcg tagttgtaca
      601 tccggacagt gccctcctcc tcacccaggg agatgaggta gatcagggca ttgaccagct
      661 cctggtagtt ctccacccga aggatggagg agtccccctc ggcagggggc gtggcgctgt
      721 ggggagtcac gctggtgtag ctgcgggcga gcagtgagga gcacccggac agcagcaggg
      781 acagcgcggc cacggccgcg cagagcctcc atttcatcag atccctcctc ccatgttcac
      841 gccggggctc acatccccag cgcctctccg atcagtacga cttccatctc ctctgtggca
      901 ttgggacgag cccaaagaac cgcaagcccc cggcagatgc gctctgggct cttgcagtca
      961 tagcaccggt ctgccttggc ggcgcagggg gtcctgcacc ccatggactg tgcccgcttg
     1021 ggggcggcca cgttccgggc ccgccacagt gccgcctcaa agtcgggggc gaccttgttg
     1081 acacccacca ggaagtagac cttctggtgg ccgtacaggg tggaggagac ccggttgccg
     1141 ttgccatcga tgttgacgat ctccccggtc tccgccagcg cgttggcgga ggtgatgtac
     1201 acatcggtga ggacggcgtc cctcagggcg cccccctccc agtgccagac cacctggttg
     1261 tgggcagcca gcctggggta gagccccatc tccttcaccg tcatggaccc gccgatgccc
     1321 accgtttttc catcgatctg gccgtccaga taggcggccg cctcatctgc ggtggggaag
     1381 taggtggtgc ggaagccgcg ggcctccagg ttggcgcgga gtttttccag atctgccatg
     1441 gtgatacctc cctgaaaaaa tgaatggatc acaggtcatg ctggtgatag ctgtggaagc
     1501 cctggcccag cacctcgtgg gcatcgcaca cgatgagaaa ggcggagggg tcggtctcct
     1561 ttaccgcccg cttcagggcc
//
LOCUS       DBMDSJ010000426         1577 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16958_length_1577_cov_1.754065, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000426 DBMDSJ010000000
VERSION     DBMDSJ010000426.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16958_length_1577_cov_1.754065"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="ACZPAQ_08880"
     CDS             complement(<1..1045)
                     /locus_tag="ACZPAQ_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR0904144.1"
                     /translation="MKEVKQPSKKPLVYYYLVALLILMVLNLTLFPAMLETPVQDVTY
                     DQFMSMTYEQNVGQVQIEGDEITFTDKTNEKVYRTTATNDPDRTQRLYENGAQFQEID
                     TQPGLLTSILVGWVLPLLPFLLIGWFINRRMKKSMGGDGMMFGMGGGSNAKMYVPSST
                     GIRFADVAGEDEAKELLSEIVDFLHDPEKYRQIGAKLPKGALLVGPPGTGKTLLAKAV
                     AGEANVPFFSIAGSEFVEMFVGRGAAKVRDLFKQANDKAPCIVFIDEIDTIGKKRDGQ
                     IGGNDEREQTLNQLLTEMDGFDGSKGVVVLAATNRPDSLDPALLRPGRFDRRIPVELP
                     DLQGRIDILKVHAK"
     gene            complement(1042..1527)
                     /locus_tag="ACZPAQ_08885"
     CDS             complement(1042..1527)
                     /locus_tag="ACZPAQ_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024854.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0904145.1"
                     /translation="MLSPLETALTGQPFRRLYCRMLDELAQEWSLSRTEAEVMFLLSC
                     DKTLDTARDICETQLLKKSAVSRAAEGLVRRGWLEALPDPRDRRIQHLRLLSPARPFS
                     EEVRRAGEAFFCLLLQDVTLEEQQQMEKIAQKIEHNVRTVLSQSDSIPHILMKKDGKP
                     L"
BASE COUNT          289 a          465 c          506 g          317 t
ORIGIN      
        1 gcttggcgtg gaccttcagg atatcgatgc ggccctgaag gtcgggcagc tccaccggga
       61 tgcgccggtc gaaccggccg gggcgcagca gggcggggtc cagactgtcc gggcggttgg
      121 tggcggccag caccaccacg cccttggagc cgtcaaagcc gtccatctcg gtgagcagct
      181 ggttcagggt ctgctcccgt tcgtcattgc cgccgatctg gccgtcccgc ttcttgccga
      241 tggtgtcgat ctcgtcgatg aacacgatgc agggggcctt gtcattggcc tgcttgaaca
      301 ggtcccgcac cttggcggca ccccggccca cgaacatctc cacgaactcg ctgccggcaa
      361 tggagaagaa gggcacgttg gcctcgccgg ctaccgcctt ggccagcagg gtcttgccgg
      421 tgcccggagg gcctaccagc agggcgcctt tgggcagctt ggcgccgatc tggcggtact
      481 tctccgggtc gtggaggaag tccacgatct cgctgagcag ttccttggcc tcgtcctcgc
      541 cggccacgtc ggcaaagcgg attccagtgg atgaggggac atacatcttg gcattagagc
      601 cgccgcccat gccgaacatc atgccgtcgc cgcccatgga ttttttcatc cgtctgttga
      661 tgaaccagcc gatgagcagg aaggggagca ggggcaggac ccagcccacc aggatggagg
      721 tgagcaggcc gggctgggtg tcgatctcct ggaactgggc cccattctca tagaggcgct
      781 gggtccggtc agggtcgttg gtggcggtgg tgcggtagac cttttcgttg gtcttgtcgg
      841 taaaggtgat ctcatccccc tcgatttgga cctggcccac attctgctca taggtcatgg
      901 acatgaactg atcgtaagtc acatcctgga ccggggtctc cagcatggcg gggaacagag
      961 tcaagttcag caccatcaga atgagcagag ccaccaggta gtaatagacc agaggctttt
     1021 tagagggctg cttcacttct ttcatagcgg tttcccatcc tttttcatca aaatgtgtgg
     1081 aatcgaatcg gactggctga gtacggtgcg cacattgtgc tctatcttct gagcgatctt
     1141 ctccatctgc tgctgttcct ccagggtgac atcctggagc agcagacaga agaacgcctc
     1201 tcctgcccga cggacctctt cgctgaaagg ccgggcaggg gacaggagtc ggagatgctg
     1261 gatccgccgg tcccgggggt cgggcagcgc ctccagccat ccccgacgca ccagaccctc
     1321 cgccgcccgg gagacagcgg attttttcag cagctgagtc tcgcagatgt cccgggcggt
     1381 gtccagggtt ttatcacagg agagcaggaa catcacctct gcctcggtgc gggagaggga
     1441 ccactcctga gccagctcgt ccagcatccg gcagtagagg cggcggaagg gctggccggt
     1501 cagggcggtc tccaaggggg agagcatagg cacacctccg atagttcaaa aagaaactgt
     1561 tctgatggga acagtat
//
LOCUS       DBMDSJ010000427         1574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_16997_length_1574_cov_1.391039, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000427 DBMDSJ010000000
VERSION     DBMDSJ010000427.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16997_length_1574_cov_1.391039"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="ACZPAQ_08890"
     CDS             complement(<1..197)
                     /locus_tag="ACZPAQ_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR0904146.1"
                     /translation="MSKTVIRRVEPRSPAAKAGVQAGETLVEINGKQILDVLDYKFYS
                     YDARLTLTLQAPDGASRTLRVR"
     gene            624..>1574
                     /locus_tag="ACZPAQ_08895"
     CDS             624..>1574
                     /locus_tag="ACZPAQ_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HIR0904147.1"
                     /translation="MLDDKLKALWTRISPPQKAAFFGCFLSGYLVHLYVFTNIIPNSD
                     GLSRIFDPQQMTVSGRWFLHFASYFNGFTQMPAVIGLFSVLFLALAAALAVSLLGIRS
                     RALSALSGALMAAFPAVGYTFLYLFTASAYCFAIFLAVLSVSLARRGRWHWLAGCLLL
                     ACSMGTYQAYVTVAIGLSLLVIFWETLDPSASFSATLRLGVRLMLFLAAGAVLYYGIL
                     LVFLQVKDLELLSYLGMDAASSGYPFSRLPGLLLDTYKQVAAFFFLPGSANTFTSGWM
                     AVLDLAALALGGGCFLLCMGQEGLWRARWRPLGGLAMLALL"
BASE COUNT          201 a          567 c          426 g          380 t
ORIGIN      
        1 cggacccgca gggtgcggct ggctccatcc ggggcctgga gggtaagcgt cagacgggcg
       61 tcataggaat agaacttgta gtccagcaca tccaggatct gcttcccgtt gatctccacc
      121 agggtctctc ccgcctggac gccggctttg gccgccgggc tccggggctc cacccggcgg
      181 atcacggtct tgctcacagc gctccctcct cctctcaaaa aggcgttctt tatattatat
      241 cgtatccctg cccctctttt caaggaaaaa tccgtttttt ctcggatctc gccggacatt
      301 ttttgagggc gccgccgggt atccggcgcc atcggccacc ggacacctta tctttttctt
      361 gattgtacca tacccgtccc cgccgggcaa cacgtttcca ctctattctg ctctctcgcc
      421 gggtatccgg ggcacaaaaa ggatacctct cccccgccct tagttccgcc gctccggcag
      481 aacgccctct ccccggcctc cggggaaaag caccctttcc gccccggagt ttacaggatg
      541 tgaaaattat gatatgatgt tctatgattt tcccttttct ttctcaaatc cgcggctccg
      601 gccgccctgg aggcacctgc gacatgctgg acgacaagct caaagccctg tggacccgga
      661 tctccccccc gcagaaggcg gcctttttcg gctgttttct gtcgggctat ctggtccacc
      721 tctacgtctt taccaacatc atccccaact ccgacggtct cagccgcatc ttcgaccccc
      781 agcagatgac cgtctccggc cggtggtttc tccacttcgc ctcctatttc aacggcttca
      841 cccagatgcc ggcggtcatc ggcctcttct ccgtgctctt cctcgccctg gccgccgccc
      901 tggccgtctc tctgctgggc atccgcagcc gtgccctctc cgccctgtcc ggggcgctga
      961 tggccgcctt tcccgccgtg ggctacacat ttctctatct gttcaccgcc tccgcctact
     1021 gcttcgccat ttttttggcg gtcctctccg tctccctggc cagaaggggc cggtggcact
     1081 ggctggcagg ctgtctgctc ctggcctgct ccatgggcac ctatcaggcc tatgtgacgg
     1141 tggccatcgg cctgtccctg ctggtcattt tctgggagac cctggacccg tccgcctcct
     1201 tctccgccac cctgcgcctg ggggtgcggc tgatgctgtt cctggctgcg ggggccgtcc
     1261 tctactacgg catcctactg gtctttcttc aggtgaagga cctggagctg ctgtcctatc
     1321 tggggatgga tgccgccagc tccggctatc ccttctcccg cctccccggg ctgctgctgg
     1381 acacctacaa gcaggtggcg gctttcttct tcctgcccgg ctccgccaac accttcacct
     1441 ccggctggat ggccgtgctg gatctggccg cgctggcgct gggcgggggc tgcttcctgc
     1501 tgtgcatggg gcaggagggg ctctggcggg cgcgctggcg ccccctgggc gggctggcca
     1561 tgctggccct gctc
//
LOCUS       DBMDSJ010000428         1571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17033_length_1571_cov_1.365986, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000428 DBMDSJ010000000
VERSION     DBMDSJ010000428.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17033_length_1571_cov_1.365986"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="ACZPAQ_08900"
     CDS             complement(<1..882)
                     /locus_tag="ACZPAQ_08900"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0904148.1"
                     /translation="MLKVLHGADFHLDSPFSGLKPDAAARRRGEQRELLARLTDLARE
                     READLVLLSGDLLDGDLVFRETVQALARALGEIPCPVFLAPGNHDWYGPQSPYAALEW
                     PGNVHLFTGPELGAVRLPELGCTVWGAAFCAPRQSASPLRGFHAPAEGVQLAVLHGEV
                     AAQGDYGPISEEDIAASGLDYLALGHVHQYSGLRRAGETFWAYPGCPEGRGFDELGEK
                     GVLWLEVEKGRTEAEFVPLCRRRYQILSVDLTGQADPAAAVLAALPPQTGEDIYRLLL
                     TGERGLEELDLGELEREL"
     gene            complement(930..>1571)
                     /locus_tag="ACZPAQ_08905"
     CDS             complement(930..>1571)
                     /locus_tag="ACZPAQ_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR0904149.1"
                     /translation="GITPRQARGILEAGADVITLGNHTWNRLQIADFLDSDGHILRPA
                     NYAGRVPGRGFGVFRGPAGVRIGVLNLMGRLELNSNLDSPFQAADRLLRGAPCDVVLV
                     DFHAEATSEKGAMAWYLDGRAQAVWGTHTHVPTADCQLLPKGTGFVTDLGMTGPYRSV
                     LGIRPEHSMNLFLGGLPRRYEEAEGPCKLEACRFTIDTEKKRCVEVRRADLWE"
BASE COUNT          229 a          595 c          524 g          223 t
ORIGIN      
        1 gagctcccgc tccagctccc ccagatccag ctcctccagt ccccgctccc cggtgaggag
       61 gaggcggtag atgtcctccc ccgtctgtgg gggcagcgcg gccagcacgg cggcggcggg
      121 gtccgcctgc cccgtcaggt ccacggacag gatctgatac ctccggcggc acagggggac
      181 gaattccgcc tctgtccgcc ccttctccac ctccagccag agcacgccct tttctcccag
      241 ctcgtcaaag ccacgcccct cggggcagcc ggggtaagcc cagaaagtct cccctgcccg
      301 gcgcaggccg ctgtactggt ggacatggcc cagggccagg tagtccagcc cgctggcggc
      361 gatgtcctcc tccgagatgg ggccgtagtc cccctgggcg gccacctccc cgtggaggac
      421 ggccagctgg accccctccg ccggggcatg aaagccccgc aggggggagg cgctctgccg
      481 gggggcgcag aaggccgctc cccacaccgt acagcccagc tccggcagcc gcaccgcccc
      541 cagctccggg ccggtgaaca ggtggacgtt ccccggccac tccagcgcgg cataggggga
      601 ctggggaccg taccagtcgt ggttgcccgg ggccaggaat acggggcagg ggatctctcc
      661 cagggcccgg gccagggcct ggacggtctc ccggaacacc aggtccccgt ccagcaggtc
      721 cccggagagg agcaccaggt cggcctcccg ctcccgggcc agatcggtca gccgggccag
      781 cagctcccgc tgctcgcccc gccgccgggc ggccgcatca ggcttcaggc cggaaaaggg
      841 gctgtccaga tggaagtcag ccccgtggag cactttcagc atggttccat tcctttcttg
      901 gtcgttcaaa atgggccggt ccgccggcat cactcccaga ggtccgcccg gcgcacctcc
      961 acgcaccgct tcttctccgt gtcgatggtg aagcggcagg cctccagctt gcaggggccc
     1021 tccgcctcct cataccggcg gggcagcccg cccaggaaca ggttcatgga gtgctccggc
     1081 cggatgccca gcaccgagcg gtaggggccc gtcatgccca ggtcggtgac gaaccccgtc
     1141 cccttgggca gcagctggca gtccgcggtg ggcacgtggg tgtgggtgcc ccacaccgcc
     1201 tgagcccggc catccagata ccaggccata gcccccttct cactggtggc ctccgcgtgg
     1261 aagtccacca gcaccacgtc gcagggggcg ccgcggagca gccggtccgc cgcctggaag
     1321 gggctgtcca agttggagtt gagctccagc cgccccatca ggttcagcac gccgatgcgc
     1381 acccccgccg gtccccggaa tacaccgaag ccccggcccg gcacccggcc ggcgtagttg
     1441 gccggccgga ggatatgacc gtcgctgtcc aggaagtccg cgatctgaag gcggttccag
     1501 gtgtggttgc ccagggtgat cacgtccgcc ccggcctcca ggatgccccg ggcctgccgg
     1561 ggggttatgc c
//
LOCUS       DBMDSJ010000429         1566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17099_length_1566_cov_1.505119, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000429 DBMDSJ010000000
VERSION     DBMDSJ010000429.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17099_length_1566_cov_1.505119"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="ACZPAQ_08910"
     CDS             complement(<1..851)
                     /locus_tag="ACZPAQ_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HIR0904150.1"
                     /translation="MEFDVLWAALHGVSAYAELLKTPVMEQSRALVGSLAQGRGTEAL
                     EAYTQLFHLLRREGYQGLGDWLWDGLRYVESPYGTLAERGDSDPALENAARREVETFL
                     LLARMDCDRYVGELSNLLPPVYAPVLAGLPRWRASAPFDFDGLTAFYQENGCGLFARY
                     RAFVWDSGELIPVADPDCPRPEELMGYELQRGQVVANTQAMLEGHLVNNVLLFGDGGT
                     GKSATVKSMLFRPGFGDLRLIEVQKEGLAQMPRLIRSLAGRRQKFILFIDDLAFDQDD
                     NTYSIMKT"
     gene            1088..>1566
                     /locus_tag="ACZPAQ_08915"
     CDS             1088..>1566
                     /locus_tag="ACZPAQ_08915"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR0904151.1"
                     /translation="MAGVTDMAFRTICREQGAALTCTEMVSAKALCYQDKKSIPLLQL
                     GDGEHPAAAQIFGSDPVCMEEAAAIARQVSGADFIDINMGCPVPKVANSGDGSGLMRT
                     PDKAVRVAEAVIRGAGCPVTVKFRLGWDKGSINCVEFARAMEEAGVAAVAVHGRTRA"
BASE COUNT          277 a          534 c          492 g          263 t
ORIGIN      
        1 gtcttcatga tggaataggt attgtcgtcc tggtcgaaag ccaggtcgtc gatgaagagg
       61 atgaacttct gccgccgtcc cgccagggag cggatcagcc ggggcatctg ggccagcccc
      121 tccttctgca cctcgatcag ccgcagatcc ccgaagccgg ggcggaacag catagacttt
      181 acagtggctg acttgccagt gccgccgtcg ccaaagagga gcacgttgtt caccaggtgc
      241 ccctccagca tggcctgggt gttggccacc acctgtcccc gctgcagctc gtagcccatc
      301 agctcctccg gccgggggca gtccgggtcg gccaccggga tcagctcccc gctgtcccag
      361 acaaaggccc ggtagcgggc gaacaggccg cagccattct cctggtaaaa cgccgtaagg
      421 ccgtcaaagt caaaaggtgc agacgcccgc caccggggca gcccagccag caccggagcg
      481 tacaccgggg gcagcagatt ggacagctcc cccacatacc ggtcgcagtc catccgggcc
      541 agaaggagga aggtctccac ctcccgccgg gctgcgttct ccagggcggg gtcgctgtcc
      601 cctcgctccg ccagcgttcc ataggggctc tccacatagc gcaggccgtc ccagagccag
      661 tcacccagtc cctggtatcc ctcccgccgc agcaggtgga agagctgcgt ataggcctcc
      721 agggcctccg ttccacgccc ctgggccaga ctgcccacca gggcccggct ctgctccatc
      781 actggcgtct tcagcagctc cgcataggca gatacgccat ggagggccgc ccaaagtaca
      841 tcaaattcca tcttatttct cccagttccc cctgcttcca caggtgatct cttttcattg
      901 taaagcggac agggcggcct tgtcaaccgg gtgggggtat gatacaataa ataaatctga
      961 ctcaggaaag gaagacagag gacgcggcgc gtgaaacggc ggcctgctcg ggccccgccc
     1021 cgctgtcctt gcccatatgc tgtccattgg aggcatcccc atcgaagcgc ccctggccct
     1081 gggccctatg gccggggtca ctgatatggc cttccgcacc atctgccggg agcagggcgc
     1141 cgccctgacc tgcacagaga tggtcagcgc caaggccttg tgttatcagg ataagaagtc
     1201 catccccctg ctccagctgg gggatggcga gcatcccgcc gccgcccaga tcttcggcag
     1261 cgacccggtc tgcatggagg aggcggccgc catcgcccgc caggtctccg gcgccgactt
     1321 catcgacatc aacatgggct gcccggtgcc caaggtggcc aacagcgggg acggctccgg
     1381 cctgatgcgt accccggaca aggcggttcg ggtggctgag gcggtcatcc ggggggccgg
     1441 ctgtccggtg acggtgaagt tccggctggg ttgggacaag ggttccatca actgtgtgga
     1501 gtttgcccgg gccatggagg aggccggcgt ggccgccgtg gccgtccacg ggcggacccg
     1561 ggccca
//
LOCUS       DBMDSJ010000430         1564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17128_length_1564_cov_1.190704, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000430 DBMDSJ010000000
VERSION     DBMDSJ010000430.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17128_length_1564_cov_1.190704"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>361
                     /locus_tag="ACZPAQ_08920"
     CDS             <1..>361
                     /locus_tag="ACZPAQ_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR0904152.1"
                     /translation="YFEDAAFVGHSRTDGFLLYSGIGTGTNLTSNGLSIFKLAEKKAI
                     TIDGTAYTLLEALELGEYGKVYLSLGVNELGYYNDEGFYQAYCDAIDAIRACQPDAVI
                     YIQGLIPLNESVVAASGG"
     assembly_gap    362..371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <372..613
                     /locus_tag="ACZPAQ_08925"
     CDS             <372..613
                     /locus_tag="ACZPAQ_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR0904153.1"
                     /translation="YLTNEHLRVYNGLMQKAAEEKQVVFLDLYSEFANENGELPEEAS
                     KDGVHLKSDYCKQWLEYLKVHTVDFETLYPEGTET"
     gene            610..1086
                     /locus_tag="ACZPAQ_08930"
     CDS             610..1086
                     /locus_tag="ACZPAQ_08930"
                     /inference="COORDINATES: protein motif:HMM:NF025628.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HIR0904154.1"
                     /translation="MRRFAGIAAVLFLLLLPLAACGGAEEKAGFDPEITLQALADAGA
                     FSEELEELDGDTAFTLYGLGNSGLEREDLTAAKVLRSAGATCEEGAVLVFTSGDQAQT
                     AVQALGDYVQKQIDSNRDYRPQELPKLESAVIQQRENTVLLLVAGDLEAAQGVLEQ"
     gene            1241..>1564
                     /locus_tag="ACZPAQ_08935"
     CDS             1241..>1564
                     /locus_tag="ACZPAQ_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0904155.1"
                     /translation="MRRLFFRWAAAALLAGALMIPAAEAAQLQIDDRIVEPSAAWTEE
                     GTPYVTLAALCQAADGYTLSWNGTAAALTAEDLELTATPGALYVEVNGRALYVEHGVQ
                     VRDGRI"
BASE COUNT          324 a          428 c          528 g          274 t
ORIGIN      
        1 tactttgagg acgccgcttt tgtggggcac tcccgcacgg acggcttcct gctttacagt
       61 gggatcggga ctgggaccaa cctgacctcc aacggcctgt ccatcttcaa gctggcggag
      121 aaaaaggcga tcacgattga tgggaccgcc tatacgctgc tggaggccct ggagctgggc
      181 gaatatggaa aggtgtacct gtccctgggc gtcaatgagc tgggctacta caacgacgag
      241 ggcttttacc aggcatactg tgacgccatc gatgctatcc gggcttgcca gcccgacgcg
      301 gtcatctaca tccagggcct gatccctctg aacgagtcgg tggtggcggc atctggcggc
      361 cnnnnnnnnn ngctatctga ccaacgagca cctccgggtg tacaacggcc tgatgcagaa
      421 agcggcagag gaaaagcagg tggtgtttct ggacctgtat tccgaatttg ccaatgagaa
      481 cggggaactg cccgaggagg ccagcaagga cggggtgcat ctgaagagcg actactgcaa
      541 gcagtggctg gagtatctga aagtccatac tgtggacttc gaaacacttt atccggaggg
      601 aactgagaca tgagacgatt tgctgggata gcggcggtac tgtttctgct tttgctgccg
      661 ctggccgcct gcggcggcgc ggaggagaag gctgggtttg acccggagat caccctccag
      721 gcgctggcgg acgctggagc cttctctgag gaactggagg agctggacgg cgacactgcc
      781 tttaccctct acggcttggg aaactccggg ctggagcggg aggatctgac cgccgccaag
      841 gtgctgcgct cagcgggcgc cacctgtgag gagggggctg tcctggtctt caccagcggg
      901 gaccaagcgc agacagccgt ccaggcgctg ggagactatg tgcagaagca gatagactcc
      961 aaccgggact accgtcctca ggagctgccc aagctggaga gcgccgtcat ccagcagcgg
     1021 gagaacactg tgctgctgct ggtggccggt gacctggagg cggctcaggg cgtgctggag
     1081 cagtaaaggg agatagactg gggggcgggc cgcagatgcg gcccgcccct tttatgcctg
     1141 actgcctgga agaaaacgga gcaccaaaac ttacagcttg cctttgcacc acataaaagt
     1201 tacaatttgg ttacaaaata gagcaaagga gcacgagagc atgagacgac tgtttttccg
     1261 atgggccgcg gccgccctgc tggcgggcgc tctgatgata ccggccgccg aagccgccca
     1321 actccagatc gatgacagga tagtggagcc ctccgccgca tggacggagg aggggacgcc
     1381 ctatgtgacg ctggccgccc tgtgccaggc ggcggacgga tacacgctga gctggaatgg
     1441 gacagcggcg gcgctgacag cggaggacct ggaactgacg gccacccccg gcgccctcta
     1501 cgtggaggtc aacggcagag ccctgtatgt ggagcacggg gtacaggtcc gggatgggag
     1561 gatc
//
LOCUS       DBMDSJ010000431         1553 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17280_length_1553_cov_1.995868, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000431 DBMDSJ010000000
VERSION     DBMDSJ010000431.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17280_length_1553_cov_1.995868"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="ACZPAQ_08940"
     CDS             complement(<1..533)
                     /locus_tag="ACZPAQ_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0904156.1"
                     /translation="MSQIGHELVVVGGGAAGMMAAITAARLGADVCLVERNPKLGRKL
                     YITGKGRCNVTNHCTPEEVLAATPRNGKFLYSAMNQTPPAEVERFFTELGVELKVERG
                     NRVFPVSDRSADIIDALFFELRRLSVPVIQGRVSALDVQDGAVCAVELRQDGEGQKKI
                     PCRGVVLATGGASYPATG"
     gene            complement(530..1393)
                     /locus_tag="ACZPAQ_08945"
     CDS             complement(530..1393)
                     /locus_tag="ACZPAQ_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR0904157.1"
                     /translation="MNRDILAVIQENMDSFSKGQKRIANYILESYDKAAFMTASKLGK
                     RVSVSESTVVRFAAELGYDGYPSMQKSLQKMIRNRLTSVQRIEVANDRIGDQDVLDMV
                     LQSDIDKIRQTLDEIDRDSFENATDAIVAARRIYVIGVRSAAAIATFLGFYFNLIFDN
                     VVMITANTPSEVFESLLRVGEEDVIIGVSFPRYSSRAVQAVSFARDRGATTIAITDSE
                     ASPLAQISQYTLKARSDMASFVDSLVAPLSLVNALLVEVSRKKNEDLSKTFQTLEKIW
                     EEYGVYEKVQE"
BASE COUNT          281 a          522 c          443 g          307 t
ORIGIN      
        1 ccggtggcgg gataggacgc cccgccggtg gccagcacca ccccgcggca ggggatcttc
       61 ttttgtcctt ccccgtcctg cctcagctcc acggcacaca ccgccccgtc ctgtacgtcc
      121 agtgcggaga cccggccctg gatgacgggc accgacaggc ggcgcagttc aaaaaacagg
      181 gcgtcaatga tgtcggcgga ccggtcggag acggggaaca cccggttgcc ccgctccacc
      241 ttcaattcca cgcccagctc tgtaaagaag cgctccacct ccgccggcgg cgtttgattc
      301 atggcgctgt aaaggaattt tccatttcgg ggtgttgcag ccagcacttc ctccggcgta
      361 cagtggttgg tgacgttgca ccgccccttc cccgtaatgt acagcttccg tcccagcttg
      421 gggttgcgct ccaccaggca gacgtccgcg cccaggcggg cggcggtgat ggccgccatc
      481 atacccgcgg ctccgccccc caccacaaca agttcatggc cgatctgact cactcctgca
      541 ccttctcata gaccccatac tcctcccaga tcttctccag cgtctggaag gtcttggaca
      601 ggtcctcgtt cttctttcgg ctcacctcta ccagcagggc gttcaccagg ctcagcgggg
      661 ccaccaggga atccacaaag gaggccatgt cgctgcgggc cttcagcgta tactgggaga
      721 tctgagccag gggggacgcc tcgctgtccg tgatagcgat ggtagtggcc ccccggtccc
      781 gggcaaagct caccgcctgg accgcccggc tggagtagcg gggaaagctg acgccgatga
      841 tgacgtcctc ctcccccacc cgcagcaggc tttcaaacac ctcgctgggg gtatttgcgg
      901 tgatcatgac cacattgtca aagatcagat tgaagtagaa gcccaggaac gtggcgatgg
      961 ccgccgcgga gcgcacgccg atcacataga tccggcgggc cgccacaatg gcgtcggtgg
     1021 cattttcaaa gctgtctcgg tcgatctcat ccagggtctg gcggatcttg tcgatatccg
     1081 actggagcac catatccagc acatcctggt cgccgatccg gtcgttggcc acctcgatgc
     1141 gctgcaccga agtcaggcgg ttccggatca ttttttgcag ggacttctgc atactggggt
     1201 atccgtcata gcccagctcc gccgcgaagc gcaccacagt ggactcgctg acgctgaccc
     1261 gcttgcccag cttgctggcc gtcataaagg ccgccttgtc ataggactcc agaatgtagt
     1321 tggcgatccg cttctgcccc ttggagaagc tgtccatatt ttcctggatc acagccagaa
     1381 tatcacgatt cattccgtct catcctcccg caggcccccg gacgcgcaaa acgcccgggc
     1441 gcacggcttt gccggcgtcc ggactgctcc gcccctccgg gagatcagtt tccttgttct
     1501 ccatgataac ggttccgggg gaaaaatgaa actgatctcc cggaggggcg gag
//
LOCUS       DBMDSJ010000432         1551 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17305_length_1551_cov_2.117241, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000432 DBMDSJ010000000
VERSION     DBMDSJ010000432.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17305_length_1551_cov_2.117241"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="ACZPAQ_08950"
     CDS             <1..677
                     /locus_tag="ACZPAQ_08950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0904158.1"
                     /translation="FRPEEGGFAVETDGGPLHCRALILAPGTRHRPLGLEGEEALTGQ
                     GVSYCAVCDGPFYQGRDTAIVGGGDTALQEALFLSGVCRRVTLIHRRLEFRGSPKLAE
                     RVRTRDNIRLLLGYTPQALLQAEGRLTGLRVRQASTGQTLELPLDGVFAAVGRVPGTA
                     PFRAQLELDDEGYFRSGEDCRTRLPGVFVAGDCRAKEVRQLATAVGDGAVAGLAAARF
                     VEDGQG"
     gene            complement(746..>1551)
                     /locus_tag="ACZPAQ_08955"
     CDS             complement(746..>1551)
                     /locus_tag="ACZPAQ_08955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0904159.1"
                     /translation="DPQLLNCVVVKVPTYEEDNYRLRVEEYEKRLTPKTKMVLLSSPN
                     NPTGRVFTREELTELSQFIIKHDLICVCDQAFEDTAFPGHEMVTMASLPGMWERTVTV
                     CSCSKGMALSGYRVGYIFADDVIMDTMYATAVNIQGAANTMVQMAMIPAYEDTSFIQD
                     YVAEQDFRRRYAYEKFNAVPGVSMPLMESGFYGWVNVSRLGDSSEITAYLAEEAKTLV
                     NDGKFYGDQGAGHLRIIYGVFADRQKSLDGIDRLCEALAKRAAQLGITE"
BASE COUNT          251 a          521 c          502 g          277 t
ORIGIN      
        1 gcttccgccc ggaggagggg ggctttgccg tggagaccga cggcggcccc ctccactgcc
       61 gcgccctgat cctcgcccct ggcacccggc accgccctct gggcctggag ggggaggagg
      121 ccctcaccgg acagggggtg tcctactgcg ccgtgtgtga cggccccttc tatcagggcc
      181 gggacaccgc catcgtgggg ggcggggaca ccgccctgca ggaggccctg ttcctctccg
      241 gcgtgtgccg ccgggtgacc ctgatccacc gccggctgga gttccggggc agtccaaagc
      301 tggcggagcg ggtacggacc cgggacaata tccgcctgct gctgggctat acaccgcagg
      361 ccctgctcca ggcggagggc aggctcaccg gtctgagggt ccgccaggcc tccaccggac
      421 agaccctgga gctccccctg gacggggtgt tcgccgccgt ggggcgggtc cccggcaccg
      481 cccccttccg ggcccagctg gagctggacg atgagggcta tttccgctcc ggtgaggact
      541 gccgcacccg cctccccggc gtctttgtgg ccggggactg ccgggccaag gaggtgcgcc
      601 agctggccac cgctgtgggc gacggcgcgg tggccggcct ggccgccgcc cggttcgtag
      661 aggacggaca aggctgaatc ttcaccctcc aaaacgcaga tctccctccc cgccggagac
      721 accggcaggg agggagatct ctttcttatt ctgtgatgcc cagctgggcg gcccgcttgg
      781 ccagagcctc gcacagacgg tcgatcccgt ccaggctctt ctgtcggtca gcgaacacac
      841 cgtagatgat gcgcaggtgg ccggctccct ggtctccgta aaattttccg tcgttgacca
      901 gcgtcttggc ctcctccgcc agataggcgg tgatctcgct ggagtctccc aagcggctga
      961 cattgaccca gccatagaag cccgactcca tcaggggcat ggatactccg ggcaccgcat
     1021 tgaatttctc ataggcatag cggcggcgga agtcctgctc cgccacatag tcctgaatga
     1081 aggatgtgtc ctcataggcg gggatcatgg ccatctggac catggtattg gccgcaccct
     1141 ggatattcac cgcagtggcg tacatggtgt ccatgatcac atcatccgcg aagatatagc
     1201 ccacccggta gccgctcagg gccatgccct tggagcagga acagactgtg accgtgcgct
     1261 cccacatgcc gggcagtgag gccatggtga ccatctcatg tcccggaaac gcggtatcct
     1321 caaaggcctg atcgcagaca cagatcaggt catgcttgat gatgaactgg gacagctctg
     1381 tcagctcctc ccgggtgaac acccggccgg tggggttgtt gggggaggag agcagcacca
     1441 tcttcgtctt gggcgtcagc cgcttctcat actcctccac ccgcaggcgg tagttgtcct
     1501 cctcataggt gggcaccttc accaccacgc agttgagcag ctgggggtcc a
//
LOCUS       DBMDSJ010000433         1542 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17430_length_1542_cov_2.027065, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000433 DBMDSJ010000000
VERSION     DBMDSJ010000433.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17430_length_1542_cov_2.027065"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="ACZPAQ_08960"
     CDS             complement(<1..746)
                     /locus_tag="ACZPAQ_08960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR0904160.1"
                     /translation="MTFQSLGLCAPILAALKEQGYEAPSPIQEQAIPPALAGRDVLGC
                     AQTGTGKTCAFASPILQRLSAARTPDHRLRALILTPTRELAIQIGESFAAYGAHLSLR
                     HTVIFGGVGQNPQVEALGRGVDILVATPGRLMDLYQQGFVRLEDLEIFVLDEADRMLD
                     MGFIHDVKKILKWLPAKKQTMLFSATMPPEIAALVNSLLHDPARVAVDPVSSPVETIQ
                     QSVYFVDKGNKTKLLALLIGQLDVKNALVFT"
     gene            complement(837..1346)
                     /locus_tag="ACZPAQ_08965"
     CDS             complement(837..1346)
                     /locus_tag="ACZPAQ_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR0904161.1"
                     /translation="MSNITLIGMPGSGKSTVGVLLAKALGRGFLDTDLVIQQQENALL
                     QDILDRRGVTYFLDAEERAVRSVRCDHCVIAPGGSVVCREGAINHLKSMGPVIYLRVP
                     LDELKSRIHNLDSRGIALEPGQTLEDILTLRAPLYEQYADGVVDALPGQELAQTVSHV
                     LALLEKGLP"
BASE COUNT          292 a          449 c          506 g          295 t
ORIGIN      
        1 gtaaatacca gggcattttt cacatccagc tgtccaatca gaagggccag cagtttggtc
       61 ttattgccct tatccacaaa atagacggac tgttggatgg tctccaccgg ggaggacacc
      121 ggatccaccg ccacccgggc cgggtcgtgc agcagggagt tgaccagagc ggcgatctct
      181 gggggcatgg tggcagagaa gagcatagtc tgctttttgg cgggcagcca cttcaggatc
      241 tttttcacgt cgtggataaa gcccatgtcc agcatccggt cggcctcatc cagaacgaag
      301 atctccagat cctccagccg gacgaagccc tgctgataca ggtccatcag acggcctggc
      361 gtggccacca gaatatccac accgcggccc agggcttcca cctgagggtt ctgccccact
      421 ccgccgaaaa tgacggtgtg gcgcagggat aggtgtgctc cgtaggcggc gaaactctcg
      481 ccgatctgga tggccagctc ccgggtgggt gtgaggatca gggcgcggag ccggtggtcc
      541 ggcgtccggg ccgcgctcag ccgctggagg atgggggagg caaaggcaca ggtctttcca
      601 gtgcctgtct gggcgcagcc cagtacgtcc cgtcccgcca gggcaggagg gatggcctgc
      661 tcctgaatgg gggagggggc ctcatagccc tgctccttta gagcagctaa aatgggggcg
      721 cacagcccca gggattgaaa tgtcatgttg ttcttccttc tttttgttcc ggcccgtggg
      781 ggatccaagg gcggcccgcg ttttccggcc tggagccgga gccccgcggg ctggggtcag
      841 gggaggccct tctccagaag ggccagcaca tgagagacag tctgagccag ctcctgtccc
      901 ggcagggcgt ccaccactcc gtcggcgtac tgctcgtaca ggggggcgcg gagggtgagg
      961 atgtcctcca gcgtctggcc gggctccagg gcgatccccc gggagtccag gttgtggatg
     1021 cgggacttca gctcatccag gggcacccgg agatagatca cgggccccat ggacttcaga
     1081 tgattgatcg cgccttcccg gcagaccacg ctgccgccgg gggcgatgac gcagtggtca
     1141 cagcggaccg agcgcaccgc gcgctcctcc gcgtccagaa aataggttac gccccggcgg
     1201 tccaggatgt cctggagcag ggcgttctcc tgctgctgga tgaccaagtc ggtgtcaaga
     1261 aagccgcggc ccagcgcctt ggccagcagg acgcccacag tgctctttcc ggagcctggc
     1321 atcccaatca gtgtgatgtt gctcacagtc ccattcctct catgctgctt tggtaacagc
     1381 cattagttta ccatagtttt tacaaaaaag aaagtaggaa aagcagtgga cagggatttc
     1441 tgggggggaa agctggctgc tgtcccggga gggcgggagg tcgctgcctc ggtagaaaat
     1501 tttattgcaa tcggcggcgg ttcacgctaa aatagacgtg at
//
LOCUS       DBMDSJ010000434         1537 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17502_length_1537_cov_1.198468, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000434 DBMDSJ010000000
VERSION     DBMDSJ010000434.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17502_length_1537_cov_1.198468"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="ACZPAQ_08970"
     CDS             complement(<1..386)
                     /locus_tag="ACZPAQ_08970"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008719705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR0904162.1"
                     /translation="MPEEKMHFKDYRSGMLTNGIERAAHRALLYSTGLDREDLGKPMI
                     AVVNSFTEMVPGHFHLRELADFVKQGIIEAGGIPREFSTIAICDGICQGHKGMSYPLP
                     SRELIADSAEYMIEAHQFDGMVMLPG"
     gene            510..1433
                     /locus_tag="ACZPAQ_08975"
     CDS             510..1433
                     /locus_tag="ACZPAQ_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015431.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0904163.1"
                     /translation="MNISLEYFLAVAEEESISRAAERVMVSQQDMSNHIRRLEKQYGL
                     LFERRPRFALTPAGEAVLATYRQVRLLEESLEDRLRDLREDTEGTIRLGMHIARARIL
                     VPPAAARFCREFPHVRLEVVHGDTAVLEDKLGQGTLHLFQGVDPEERPHFCFTPVGSE
                     TVYLVIAGALLDRCFPQGGAGDTIDETQLSRLPLIFSPTISKTQARITDFFRQHGLAT
                     AFQITAGDYDLQLRLAAQGLGACFCPAMHLRDVRHLLEGPEPILRRLYVPDLDVRNEL
                     KLVTNRRMYHPRYLDVFAGILEEEARRALLC"
BASE COUNT          280 a          457 c          498 g          302 t
ORIGIN      
        1 cagccgggga gcatcaccat gccgtcgaac tgatgggcct cgatcatgta ctcggcggag
       61 tcggcgatga gttcccggct gggcagggga tagctcatcc ctttgtgccc ctggcagatg
      121 ccgtcgcaga tggcgatggt gctgaactcc cgggggatgc cccccgcctc gatgatcccc
      181 tgcttaacga agtccgccag ctcccgcagg tgaaagtggc ctggtaccat ctcggtgaaa
      241 gagttgacca ctgcgatcat gggtttgccc aggtcctccc ggtccagacc ggtgctgtac
      301 agcagcgccc ggtgagccgc ccgctcaatc ccgttggtca gcatcccgct tcggtaatcc
      361 ttaaagtgca tcttctcctc tggcatgttc agcgctcctt tcctgtgcgg ctgttttcat
      421 ctagcagtgt accagcggat tgacaaaacc gcaaacacat tttatattta ttttataaac
      481 aaaaaagttg tatttaggga gggagagcgg tgaatatcag tctggaatac tttctggccg
      541 tggcggaaga ggagagcatc agccgggctg cggagcgggt catggtgagc cagcaggata
      601 tgagcaacca tatccggcgt ctggaaaagc agtacgggct cctgtttgag cggcggccca
      661 gatttgccct gaccccagca ggggaggccg tgctggccac ctaccggcag gtgcggctgc
      721 tggaggagag cctggaagac cggctccggg acctgaggga ggatacagag gggaccatcc
      781 gcctggggat gcacatcgcg cgggcgcgca tcctggtccc cccggcggcg gccagattct
      841 gccgggagtt cccccacgtg cgcctggagg tggtccacgg ggacacggcc gttctggaag
      901 acaagctggg gcaggggacg ctgcacctgt tccagggtgt agatccggag gagcgtcccc
      961 acttctgctt cacgccggtg ggctcggaga cagtctatct cgtgattgcc ggggccctgc
     1021 tggaccgctg cttccctcaa gggggcgcgg gggataccat tgacgagacg cagctatcgc
     1081 gcttaccgtt gatcttcagc ccgaccatca gcaagactca ggcccggatc acagactttt
     1141 tccggcagca cgggctcgcc accgcatttc aaatcacggc gggggactat gacctccagc
     1201 tccggctggc ggcccagggc ctgggggcct gcttctgccc ggctatgcac ctgcgggacg
     1261 tccggcacct tttggagggt ccggagccca ttcttcggcg gctgtatgtc ccggatctgg
     1321 acgtgcgtaa cgaactgaag ctggtgacca accggcggat gtatcacccc cgctatctgg
     1381 acgtctttgc cgggatattg gaggaggagg ccaggcgcgc cctattgtgc tgagcgtccc
     1441 attccaggcc ggacatgggc caatgggctc caaagccgcc ccctccgccc gaacagggcg
     1501 gagggggcgg ctttgggcgt tagaagtatg aatagta
//
LOCUS       DBMDSJ010000435         1536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17509_length_1536_cov_2.397909, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000435 DBMDSJ010000000
VERSION     DBMDSJ010000435.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17509_length_1536_cov_2.397909"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="ACZPAQ_08980"
     CDS             complement(<1..277)
                     /locus_tag="ACZPAQ_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HIR0904164.1"
                     /translation="MSTLTRTADRPGERADAFLARVVPDLTRSAAQRLLEAGAVRLAG
                     RPVRKNYRTAPGDVLEVDIPAPQPVDLVPQDIPLDIVYEDGDVIVVNK"
     gene            complement(252..740)
                     /gene="lspA"
                     /locus_tag="ACZPAQ_08985"
     CDS             complement(252..740)
                     /gene="lspA"
                     /locus_tag="ACZPAQ_08985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR0904165.1"
                     /translation="MPYAVLAAALVALDQLVKYLVSHNIPLGGHVPLLPHLVELTYFQ
                     NTGAAFSMLEEHTWLLTLISAVMSVVLGVAVWKKFFAHPFGRCALTLVLAGAVGNLID
                     RALQGYVVDMFNVLFMDFAIFNVADICVVVGGIAACVYYVFFYEKLEGKGTLHEHADA
                     DR"
     gene            1061..>1536
                     /locus_tag="ACZPAQ_08990"
     CDS             1061..>1536
                     /locus_tag="ACZPAQ_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR0904166.1"
                     /translation="MEINGEIVNDKVRYADLNLSPELMRAIDKKGYVQATPVQAGAIP
                     YFMEWKDVIAKAPTGTGKTFAFGIPMVEHADPQGTQVTGLVLAPTRELAIQIRDELRD
                     LCAFKEGVRTVCLYGGQPIDKQILQLKKAPQIVVATPGRLMDHVKRRTVRLDKVETV"
BASE COUNT          318 a          459 c          521 g          238 t
ORIGIN      
        1 gcttgttgac cacgatcacg tccccgtcct cgtacacgat gtccaggggg atatcctggg
       61 ggaccaggtc taccggctgg ggggcgggga tgtccacctc cagcacgtct ccgggggcgg
      121 tgcggtagtt tttccgcacc ggcctgcctg ccagccgaac cgcgccggcc tccagcagcc
      181 gctgggcggc ggaacgggtc aggtcaggca ccacccgggc caggaaggcg tccgcccgct
      241 cgccgggacg gtcagcggtc cgcgtcagcg tgctcatgga gggtcccctt tccttccagc
      301 ttctcataga aaaagacata atagacgcag gcggcgatcc cgcccaccac cacgcagata
      361 tcggccacat tgaagatggc gaagtccata aaaaggacgt tgaacatgtc cactacatag
      421 ccctggagcg cccggtcgat cagattgccc accgcgcctg ccaggaccag ggtgagggcg
      481 cagcggccga aggggtgggc aaagaacttc ttccacaccg ccacgcccag caccacggac
      541 atgacggcgg agatcagggt gagcagccag gtgtgctcct ccagcatgga gaaagcggcg
      601 ccggtatttt gaaagtaagt cagctccacc aggtggggga ggagggggac atggcccccc
      661 agggggatgt tgtggctgac cagatatttc accagctggt ccaaggcgac aagggcggcg
      721 gcgagcacag cgtagggcat ggtggtcctc ctttggacag ctcccgaaaa gcgggagttt
      781 ttgttttatt ttagcacctc gcggccggga gcacaacagc gaaatgggga aaatcggctg
      841 gaaagggccg caggggcggc gcttcgcttg cttcttgggg cagactatga taaaatgata
      901 caaattcccg cacaggagcg ggcttttggg gggagggcag acctccccag ctgatttgag
      961 gccgcgccgg ggaaagggcg tgtctggtcc gcggagcacc gcgggcggtg agagggggcc
     1021 ttggatcgga ccagacatgc ggaaaaggat gaacagagat atggaaatca acggcgagat
     1081 cgtcaacgac aaggtccggt acgcggacct gaacctgtcc ccggagctga tgcgggccat
     1141 cgacaaaaag ggctatgtcc aggcaacccc ggtccaggcg ggggccatcc cctacttcat
     1201 ggagtggaag gacgtgatcg cgaaggcccc caccggaacg ggcaagacct ttgccttcgg
     1261 catccctatg gtcgagcacg ccgatcccca ggggacccag gtcaccggcc tggtgctggc
     1321 ccccacccgg gagctggcca tccagatccg ggacgagctg cgggacctgt gcgcgttcaa
     1381 ggagggggtg cgcaccgtgt gtctctacgg cgggcagccc atcgacaaac agatcctcca
     1441 gctgaaaaag gccccccaga tcgtggtggc caccccaggg cgcctgatgg accatgtgaa
     1501 gcggcgcacc gtccggctgg acaaggtgga gacggt
//
LOCUS       DBMDSJ010000436         1519 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17758_length_1519_cov_2.229901, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000436 DBMDSJ010000000
VERSION     DBMDSJ010000436.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17758_length_1519_cov_2.229901"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            161..>1519
                     /locus_tag="ACZPAQ_08995"
     CDS             161..>1519
                     /locus_tag="ACZPAQ_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015100.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR0904167.1"
                     /translation="MADLRTSVPPQLAQATSTTSIMLDVIVALIPALGMSVYLFGPRV
                     LVLTLISVGACVLSEYLYRRLTHQSNTLRDCSACVTGLLLAMCLPAASPYWVPLLGGV
                     FAIVVVKQFYGGLGKNFMNPALAGRMLLSTFPFLMTTWVDALDYLPVWGEMDAVTSPT
                     PMAALHSGRLPELDMGQMLLGQHGGSIGEVSIFMLLLGGLYLIMRRVISPRIPAAFLG
                     TVAVLTFLFPQDGTSLEWMTAQLCSGGLVLGAFFMATDYTTSPVTPRGQVLYGVGCGL
                     LTVLLRYYGSYPDGVGWAILTMNCLVWLMDRAGMPRRFGVERFTAVREGLRAAGESLS
                     EIKFVNPGRPSSLSWGTMPGERYLDQLRPQAKAAGILLAVVMVTGLGIYSTYWLTSLD
                     TQRSELRAEEALLAQVMPGAEISTETPYRAPNALSITAGYRENELLGYCVEVQTHGFS
                     GPI"
BASE COUNT          217 a          518 c          480 g          304 t
ORIGIN      
        1 ctgaaggaaa acgcggcgga gcgcctgcgc tccctccatc cgcaggactg catggactgc
       61 ggatgctgca cctatatttg tccggcccat atccctctgg ccgacctggt gcgccaggcg
      121 gctggtcagg tgcgggaact ggagaaaggg ggggacctcc atggctgatc tccgcacctc
      181 tgtcccgcct cagctggcac aggccaccag cactacctcc atcatgctgg atgtgatcgt
      241 ggccctgatc cccgccctgg gcatgtccgt ctatctcttc ggtcccagag tgctggttct
      301 caccctgatc tcggtgggcg cctgcgtcct gtcggagtat ctctaccggc ggctgaccca
      361 ccagtccaac accctgcggg attgctctgc ctgtgtcacc ggcctgctgc tggccatgtg
      421 tctgccggcg gcctccccct actgggtccc cctgctgggg ggtgtctttg ccatcgtggt
      481 ggtgaaacag ttctacggcg ggcttggcaa aaacttcatg aacccggctc tggctgggcg
      541 gatgctgctg tccaccttcc ccttcttgat gaccacctgg gtggacgcgc tggactatct
      601 ccccgtttgg ggtgagatgg acgccgtcac ctcccccacc cccatggccg ccctgcacag
      661 cggcagactg cccgagctgg acatggggca gatgctgctg ggccagcacg gcggatccat
      721 cggggaggta tctatcttta tgctgctgct gggcggcctg tacctgatca tgcgcagggt
      781 catctcccca cgcatcccgg cggcctttct ggggaccgtg gccgtcctca ccttcctctt
      841 cccccaggat ggcacctccc tggagtggat gacggcccag ctgtgcagcg gcgggcttgt
      901 gcttggggct ttctttatgg cgaccgacta caccacctcc cctgtcaccc cccggggaca
      961 ggtactatac ggggtgggct gcggcctcct caccgtactg ctccggtact atggctccta
     1021 ccccgacggg gtgggctggg ctatcctgac catgaactgc ctggtgtggc tcatggaccg
     1081 ggcggggatg ccccgccggt tcggggtgga gcgcttcacc gccgtgcggg aggggctccg
     1141 cgccgcaggg gagagcctgt ctgagatcaa atttgtcaat ccgggccgcc cctcctccct
     1201 gtcctgggga accatgcccg gcgagcgcta tctggaccag ctccggcccc aggccaaggc
     1261 cgccggcatc ctgctggcgg tggtgatggt caccggtctg gggatctact ccacctactg
     1321 gctcaccagc ctggacaccc agcgctctga gctgcgggcg gaggaggccc tgctggccca
     1381 ggtgatgccg ggggctgaga tcagcactga gaccccttac cgcgccccaa atgccctgag
     1441 catcacggca gggtaccggg aaaatgagct gctggggtac tgtgtggagg tccagaccca
     1501 tggcttcagc ggccccatc
//
LOCUS       DBMDSJ010000437         1512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17858_length_1512_cov_1.989369, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000437 DBMDSJ010000000
VERSION     DBMDSJ010000437.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17858_length_1512_cov_1.989369"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="ACZPAQ_09000"
     CDS             complement(<1..1298)
                     /locus_tag="ACZPAQ_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0904168.1"
                     /translation="MGSIWSQTCSIGRRPPLRGHLKTQAAVVGAGMAGVLTAYALQQA
                     GLSVVVLEADRIAGGQTQNTTAKITSQHGLIYSRLIQSFGEETARRYAKANQDAVRAY
                     QDLIDVQGISCDWEERCAYLYGPDVTVLQEEAEAARRLGLPASFTREVSIPIPHDGAV
                     CFEGQAQFHPLKFLKVLADQLTIYEQSRVLTAEGDLLTTELGSVQAEHIVFACHFPFV
                     NFPGMYFARMHQERSYVLALQGAPPVDGMFLGVGRDTFSLRTYGELLLLGGGGHRTGE
                     NTEGGRYDLLRRKAREWFPQSQETAHWSAQDCIPPDHVPYIGPYSSSHPGWYVATGFQ
                     KWGMTSSMAAATLLCDMIQGRDNPYAGLFSPSRLDPAALPGILTEGGQAVKSMVKRFF
                     QIPAEAAKDIPAGHGGIVFLNGKKAGVYRDKSGALHPVDIR"
BASE COUNT          288 a          477 c          476 g          271 t
ORIGIN      
        1 cggatgtcca cggggtggag cgcccccgac ttgtcccggt agaccccggc ctttttcccg
       61 ttgaggaaca cgatcccgcc gtgtccggcc gggatatctt tggccgcctc cgctgggatc
      121 tggaagaatc gcttgaccat actcttgacg gcctgcccgc cctccgtcag gatgccgggc
      181 agcgccgcgg ggtccagacg gctgggagag aaaaggccgg cataggggtt gtccctgccc
      241 tggatcatat cacacaggag ggtggcggcc gccatggagg aggtcatacc ccacttctgg
      301 aacccagtgg ccacatacca gccgggatgg ctggaagagt aggggccgat atagggcaca
      361 tggtcaggcg gtatgcagtc ctgggcagac cagtgggcgg tctcctggct ctgggggaac
      421 cactcccggg ccttccgccg gagcaggtca taccggcccc cctcggtgtt ctcgccggtg
      481 cgatggcctc cgccgcccag caggagcaat tccccatagg tgcggagaga gaaggtgtcc
      541 cgtcccactc ccagaaacat gccgtccaca ggcggagcgc cctgcagggc cagcacatag
      601 gagcgctcct ggtgcatccg ggcgaaatac atccccggaa agttgacaaa gggaaaatgg
      661 caggcgaaaa cgatgtgctc cgcctggacc gagcccagct ctgtggtcag gaggtccccc
      721 tccgcggtga ggacccggct ctgctcgtag atggtcagct ggtccgccag cactttcagg
      781 aatttcaggg gatggaactg tgcctgccct tcaaagcaga ccgccccatc gtgggggatg
      841 gggatagaga cctccctggt gaacgaggcc ggcaggccca ggcgccgggc ggcctccgcc
      901 tcctcctgta agacagtgac gtcagggccg tagagatagg cacatcgctc ctcccagtcg
      961 caggaaatgc cttggacgtc gatcaggtcc tgataggccc ggaccgcatc ctggttagct
     1021 ttggcatacc gcctggcggt ctcctcccca aaggactgga tcagcctgct atagatcagg
     1081 ccgtgctggg aggtgatctt ggccgtggtg ttttgggtct gcccgccggc gatccggtcg
     1141 gcctccagga caacgactga aagcccggcc tgctggaggg cataggccgt cagcaccccg
     1201 gccatccccg cccctaccac cgctgcctga gttttcaaat gcccccggag gggcggcctg
     1261 cgcccaatag agcaggtctg agaccagata gatcccataa aagcctcctt tgatcctctg
     1321 cagtgaatgt ctgctttatc catgcccaac ggagcggctt ttatgtgaga gaatcactta
     1381 tagaaaaggc gaaaaacatc ggcaaggaga atgagctgcg gacagggctt cccgatgcat
     1441 cattgtattc gtccccttta tatgcagcgc acgatgcccc cagcgtgcct ccgccctgga
     1501 ggggcaggcc gc
//
LOCUS       DBMDSJ010000438         1512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Eubacteriales bacterium isolate MAG1311
            NODE_17873_length_1512_cov_0.990787, whole genome shotgun sequence.
ACCESSION   DBMDSJ010000438 DBMDSJ010000000
VERSION     DBMDSJ010000438.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508410
            Sequence Read Archive: ERR10114005
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Eubacteriales bacterium (human gut metagenome)
  ORGANISM  Eubacteriales bacterium
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.6878x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:32:42
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,803
            CDSs (total)                      :: 1,798
            Genes (coding)                    :: 1,788
            CDSs (with protein)               :: 1,788
            Genes (RNA)                       :: 5
            tRNAs                             :: 1
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 4 of 10
            Pseudo Genes (internal stop)      :: 5 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Eubacteriales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17873_length_1512_cov_0.990787"
                     /isolate="MAG1311"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:2831996"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="ACZPAQ_09005"
     CDS             complement(<1..503)
                     /locus_tag="ACZPAQ_09005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0904169.1"
                     /translation="MYDIIVVGGGPAGLTAALYAARAGKSVLVLEQETLGGQIAYAPL
                     VENYPGLPGMRGGDFADQLARQVREQGAQLRMEGVSAMRTRGEVIELTTDGGTEECLA
                     LVLATGVSHRKLGLAGEADLVGAGLSYCAVCDGAFYQGRPVAVVGGGDAALQEALFLS
                     QRCAQVTV"
     gene            complement(601..>1019)
                     /locus_tag="ACZPAQ_09010"
     CDS             complement(601..>1019)
                     /locus_tag="ACZPAQ_09010"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR0904170.1"
                     /translation="YSRITGYYRPVKNWNDGKSQEFKDRKVYDISRSSLRRAGIAGSQ
                     VTVEDHAARPVPAEELMLFATRSCPNCRQAEEQLQKAGIPYRKVLAEENPDLTTRYSV
                     RQAPTLVFEGQGGPAKVVGLGPIRQFIQENRPASVG"
     assembly_gap    1020..1029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1030..>1512)
                     /gene="nrdD"
                     /locus_tag="ACZPAQ_09015"
     CDS             complement(<1030..>1512)
                     /gene="nrdD"
                     /locus_tag="ACZPAQ_09015"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR0904171.1"
                     /translation="LTHKETQAFAKDVLNHMRERLSDYQEKYGDLYNLEATPAESTTY
                     RFAKHDKAQFPDIITANENGTPYYTNSSHLPVGYTEDIFSALDVQDELQTLYTSGTVF
                     HAFLGEKLPDWKAAAALVRKIAENYKLPYYTMSPTYSVCADHGYLAGEQHVCPHCGKE
                     A"
BASE COUNT          264 a          484 c          466 g          288 t
ORIGIN      
        1 accgtcactt gggcgcaccg ctgggacaga aagagggcct cctggagggc cgcgtcaccg
       61 ccgcccacca ccgccaccgg acgcccctgg taaaaggccc cgtcacacac cgcgcagtaa
      121 gacaggccag ctcccaccag gtcggcctcc ccggccagtc ccagcttccg gtggctcacc
      181 ccagtggcca gcaccagggc cagacactcc tccgttcctc cgtccgttgt cagctcaata
      241 acctctcctc tggtgcgcat ggcggacacg ccctccatcc gcagctgcgc cccctgctcc
      301 cggacctgcc gggccaattg gtcggcaaag tcgccgcccc tcatgccggg cagtcccgga
      361 tagttctcca ccaggggggc ataggcgatc tgaccgccca aagtctcctg ctccaggacc
      421 aggaccgact tgcccgcccg ggcagcatac agggcggcag tcagaccggc gggcccgccg
      481 cccacaacga taatatcata catatcaaag cacctcttcc tgtcaggtcg tggaaggaaa
      541 aacagccccg gaatccgggg ctgtttttat gatacacgag tttgtcctcc agcgcaaggg
      601 tcatcctaca ctggcgggcc gattctcctg gataaactgg cggatgggcc ctaggccaac
      661 caccttggcc gggccgccct gtccctcaaa gactagggtt ggggcctggc ggacgctgta
      721 tcgagtggtc aggtcgggat tttcctctgc cagcaccttc cggtagggga tgcccgcctt
      781 ctgtaactgt tcctccgcct gacggcagtt agggcaggac cgggtggcga agagcatcag
      841 ttcctccgcg ggaacaggcc gggccgcatg gtcctccacc gtcacttggc tgccggcgat
      901 gcccgcccgg cgcagagagg agcggctaat gtcgtacacc tttcggtcct tgaactcctg
      961 actcttgccg tcgttccagt tcttcaccgg gcggtagtag ccagtaatac ggctgtacan
     1021 nnnnnnnnng gcttccttgc cacagtgggg acagacgtgc tgctcccctg ccagatagcc
     1081 gtggtcggcg cagaccgagt aggtggggga catggtgtag tagggcagct tataattctc
     1141 cgcaatcttc cgcaccaggg cggcagccgc cttccagtcg ggcagcttct cccccaggaa
     1201 ggcgtggaac acagtacctg aggtatagag ggtctggagc tcgtcctgga catccagggc
     1261 ggagaagata tcctcggtgt agcccacggg cagatgggag gagttggtgt agtagggggt
     1321 cccgttctca ttggcggtga tgatgtcggg gaactgggcc ttgtcgtgct tggcgaagcg
     1381 gtaggtggtg gactcggcgg gggtggcctc caggttatag aggtcgccgt acttctcctg
     1441 atagtcgctt agacgctccc tcatatggtt gagcacgtcc ttggcaaagg cctgggtctc
     1501 cttgtgggtc ag
//
